lcf1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following lcf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lcf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100516 NM_001021624.2
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Schizosaccharomyces pombe long-chain-fatty-acid-CoA ligase Lcf1 (lcf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100516
    Clone ID Related Accession (Same CDS sequence) NM_001021624.2
    Accession Version NM_001021624.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product long-chain-fatty-acid-CoA ligase Lcf1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021624.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGGTAC AATCTAAGGC TATCTCGAAG CCGAAAGAAC ATGAGTCAGC AATCTATAGA 
    AATGCGAATT TTCCCGATCA TTTAGTAGAA ACATATAGCG ATGATGTTCA TACTCTATTC
    GACGTTTTTA GACATTCTGT CAAGCAATTT GGTAACAAGA AGGCTATGGG ATATCGCAAC
    TTGGTGAAAG AACATGTTGA GACAAAGATG GTCACGAAAG TGGTGGACGG TGAAAAAAAG
    GAGGTTCCTA AGTCCTGGTC TTATTTTGAG CTTTCAGATT ACAATTATCT TTCCTTTAAT
    GATATTTATG ACAAAGCATT GCGTTACGCC GGTGCTTTAC GTAAACTTGG TTTAAATAAG
    GGAGACAAAT TCGAACTGTA TGCTCCTACT AGCGCTTTTT GGCTTCTCAC TGCTGAGGCT
    TGTTTATCTC AATCAATGAC GATCGTAACG GCATACGATA CTTTGGGTGA AGAGGGTTTG
    CTCCATTCTT TGCGCGAATC CGGTGTCCGA GGAATGTATA CAGAAGGACA TCTCTTAAAA
    ACTTTGGTCA ATCCCTTGAA GGAAATTGAA TCACTTGAAG TAATCATCTA CCGCAACGAT
    GCCAAGGAGG AGGACATAAA GACAATTCAG GAGATTCGTC CTAACCTCAA ATTGATTAAA
    TTTGCTGATT TTGAGAAAAT GTCTCCTCCC GTTGAGCCTG ATCCACCTTC ACCTGAAGAG
    ATCTGCTGTA TTATGTACAC TTCTGGTAGT ACCGGTTTAC CTAAGGGTGT AATTTTGAGT
    CACAAGAACA TGGTTGCAAT TGTCACTGCT ATTGTCAAGC ACGTTCCTGA AGTTACCTCT
    AAAGATTATC TTTTGGCCTA CCTTCCATTG GCACACATTC TCGAATTTGC GTTTGAAAAC
    ATTTGTTTGG CTTGGGGTGG TACCATTGGC TATGCCAACG TCCGTACTTT GGTCGATACT
    AATTGCAGAA ACTGCAAGGG TGATATCAAT ACCTTCCGTC CCACTATCAT GGTTGGTGTT
    CCCGCTGTAT GGGAAATGGT ACGTAAGGGT ATCATGAGTA AATTAAACGC TGCCTCTGCT
    GTTAAGCGTT CAGTTTTTTG GACTGCTTAT TACACTAAAG CAAAGTTGAT GCGTCACAAT
    CTTCCCGGTT CTTGCGTATT GGATACCGCT GTATTCAATA AAATTAGATC TATGGGAACC
    GGTGGTCGTT TAAGATACAC TTTATCTGGT GGTTCTGCCT TATCTCCTGA CACCAAGCGC
    TTCCTTTCTA TCGTTCTCTG TCCTATGTTA ATCGGTTATG GTTTGACCGA AATTAGTGCA
    GCTGCTATGG TTCAGAACCC TGCTTGTTTC AATTTGGACG ATTCAGCTGG ATCTCTTTTG
    CCTTGTACTG AGATGAAGTT AGTTGACTGT GAAGAGGGCA ACTACAACTC TCACGGACAC
    CCTCCTCGTG GTGAAATCTG GCTTCGTGGT CCCTCTTTGA CTCGGGGATA TCTTAACCGT
    GATAAAGAAA ATAAAGAAAG CTTTACTCCA GATGGTTGGT TCCGTACTGG TGATGTTGGC
    GAATTGACTC CAGAAGGATT GCTTAGAATT ATTGATCGTA AGAAAAACCT CGTCAAAACT
    CAAAATGGCG AGTATATTGC ACTGGAGAAG CTTGAATCTC GCTATCGTAC GAGCAGTCTT
    GTTTCGAATA TCTGTGTATA TGCAGATCAG ACCAAGGTGA AGCCTTTGGC AATCATCGTT
    CCCAACGAGC CCGTCGTTCG TAAACTTGCC ACTGAACAAG CCGGTCTTAG TCCTGATGCC
    TCTTGGGAGG AAGTTTGTCA TAACAAGAAA GTTCGTCAAC TTGTTTATGA CGACCTTATT
    CGTATTGGTC GTAGTCATCA TTTCGCTAAT ATCGAACTTA TTCAAAACGT CGTCCTTGTT
    CCCATTGAGT TTACACCCGA AAATGGATTG GTGACTGCAG CTCAGAAATT ACAGCGTCGC
    AAAATTTTGG ATCGTTTCAA GAAGGAAATT GACGCCGCTT ACGCCGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595726.1
    CDS148..2178
    Translation

    Target ORF information:

    RefSeq Version NM_001021624.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe long-chain-fatty-acid-CoA ligase Lcf1 (lcf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001021624.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGAAGGTAC AATCTAAGGC TATCTCGAAG CCGAAAGAAC ATGAGTCAGC AATCTATAGA 
    AATGCGAATT TTCCCGATCA TTTAGTAGAA ACATATAGCG ATGATGTTCA TACTCTATTC
    GACGTTTTTA GACATTCTGT CAAGCAATTT GGTAACAAGA AGGCTATGGG ATATCGCAAC
    TTGGTGAAAG AACATGTTGA GACAAAGATG GTCACGAAAG TGGTGGACGG TGAAAAAAAG
    GAGGTTCCTA AGTCCTGGTC TTATTTTGAG CTTTCAGATT ACAATTATCT TTCCTTTAAT
    GATATTTATG ACAAAGCATT GCGTTACGCC GGTGCTTTAC GTAAACTTGG TTTAAATAAG
    GGAGACAAAT TCGAACTGTA TGCTCCTACT AGCGCTTTTT GGCTTCTCAC TGCTGAGGCT
    TGTTTATCTC AATCAATGAC GATCGTAACG GCATACGATA CTTTGGGTGA AGAGGGTTTG
    CTCCATTCTT TGCGCGAATC CGGTGTCCGA GGAATGTATA CAGAAGGACA TCTCTTAAAA
    ACTTTGGTCA ATCCCTTGAA GGAAATTGAA TCACTTGAAG TAATCATCTA CCGCAACGAT
    GCCAAGGAGG AGGACATAAA GACAATTCAG GAGATTCGTC CTAACCTCAA ATTGATTAAA
    TTTGCTGATT TTGAGAAAAT GTCTCCTCCC GTTGAGCCTG ATCCACCTTC ACCTGAAGAG
    ATCTGCTGTA TTATGTACAC TTCTGGTAGT ACCGGTTTAC CTAAGGGTGT AATTTTGAGT
    CACAAGAACA TGGTTGCAAT TGTCACTGCT ATTGTCAAGC ACGTTCCTGA AGTTACCTCT
    AAAGATTATC TTTTGGCCTA CCTTCCATTG GCACACATTC TCGAATTTGC GTTTGAAAAC
    ATTTGTTTGG CTTGGGGTGG TACCATTGGC TATGCCAACG TCCGTACTTT GGTCGATACT
    AATTGCAGAA ACTGCAAGGG TGATATCAAT ACCTTCCGTC CCACTATCAT GGTTGGTGTT
    CCCGCTGTAT GGGAAATGGT ACGTAAGGGT ATCATGAGTA AATTAAACGC TGCCTCTGCT
    GTTAAGCGTT CAGTTTTTTG GACTGCTTAT TACACTAAAG CAAAGTTGAT GCGTCACAAT
    CTTCCCGGTT CTTGCGTATT GGATACCGCT GTATTCAATA AAATTAGATC TATGGGAACC
    GGTGGTCGTT TAAGATACAC TTTATCTGGT GGTTCTGCCT TATCTCCTGA CACCAAGCGC
    TTCCTTTCTA TCGTTCTCTG TCCTATGTTA ATCGGTTATG GTTTGACCGA AATTAGTGCA
    GCTGCTATGG TTCAGAACCC TGCTTGTTTC AATTTGGACG ATTCAGCTGG ATCTCTTTTG
    CCTTGTACTG AGATGAAGTT AGTTGACTGT GAAGAGGGCA ACTACAACTC TCACGGACAC
    CCTCCTCGTG GTGAAATCTG GCTTCGTGGT CCCTCTTTGA CTCGGGGATA TCTTAACCGT
    GATAAAGAAA ATAAAGAAAG CTTTACTCCA GATGGTTGGT TCCGTACTGG TGATGTTGGC
    GAATTGACTC CAGAAGGATT GCTTAGAATT ATTGATCGTA AGAAAAACCT CGTCAAAACT
    CAAAATGGCG AGTATATTGC ACTGGAGAAG CTTGAATCTC GCTATCGTAC GAGCAGTCTT
    GTTTCGAATA TCTGTGTATA TGCAGATCAG ACCAAGGTGA AGCCTTTGGC AATCATCGTT
    CCCAACGAGC CCGTCGTTCG TAAACTTGCC ACTGAACAAG CCGGTCTTAG TCCTGATGCC
    TCTTGGGAGG AAGTTTGTCA TAACAAGAAA GTTCGTCAAC TTGTTTATGA CGACCTTATT
    CGTATTGGTC GTAGTCATCA TTTCGCTAAT ATCGAACTTA TTCAAAACGT CGTCCTTGTT
    CCCATTGAGT TTACACCCGA AAATGGATTG GTGACTGCAG CTCAGAAATT ACAGCGTCGC
    AAAATTTTGG ATCGTTTCAA GAAGGAAATT GACGCCGCTT ACGCCGAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L