GLT1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	GLT1
Entrez Gene ID	835427
Full Name	NADH-dependent glutamate synthase 1
Synonyms	MYN8.7,MYN8_7,NADH-GOGAT,NADH-dependent glutamate synthase 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001203600.1	NP_001190529.1	NADH-dependent glutamate synthase 1
NM_001203601.1	NP_001190530.1	NADH-dependent glutamate synthase 1
NM_124725.5	NP_200158.2	NADH-dependent glutamate synthase 1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG9674	NP_001246804.1
Schizosaccharomyces pombe (fission yeast)	glt1	NP_594133.1
Magnaporthe oryzae (rice blast fungus)	MGG_07187	XP_003715392.1
Neurospora crassa	NCU01744	XP_957167.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006360	XP_316385.4
Caenorhabditis elegans (roundworm)	W07E11.1	NP_509693.2
Kluyveromyces lactis	KLLA0E19625g	XP_454839.1
Eremothecium gossypii	AGOS_ADR290W	NP_984386.2
Arabidopsis thaliana (thale cress)	GLT1	NP_200158.2
Oryza sativa (rice)	Os01g0681900	NP_001043884.1
Saccharomyces cerevisiae (baker's yeast)	GLT1	NP_010110.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Chromatin immunoprecipitation analysis indicated that GTL1 interacts with a region of the SDD1 promoter containing GT3 box. EMSA was used to determine that the GT3 box is necessary for the interaction between GTL1 and the SDD1 promoter
Title: The Arabidopsis GTL1 transcription factor regulates water use efficiency and drought tolerance by modulating stomatal density via transrepression of SDD1.

The following GLT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb21327	NM_001203601.1 Latest version!	Arabidopsis thaliana NADH-dependent glutamate synthase 1 (GLT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAb21327	NM_124725.5 Latest version!	Arabidopsis thaliana NADH-dependent glutamate synthase 1 (GLT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAb21327	NM_001203600.1 Latest version!	Arabidopsis thaliana NADH-dependent glutamate synthase 1 (GLT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLT1 ( NM_001203600.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190529.1 Arabidopsis thaliana NADH-dependent glutamate synthase 1 (GLT1), mRNA.

Price & Availability↑

CloneID	OAb21327
Clone ID Related Accession (Same CDS sequence)	NM_001203600.1 , NM_001203601.1 , NM_124725.5
Accession Version	NM_001203600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NADH-dependent glutamate synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGTCGGCAG CTTCTTCTAG CTCCGTTCTT CACCTCCGAA CCAACCAACA ACTGTTATCA 
CTCCGATCTC TCAAAAATTC CACCTCCGTT GCTTCTCAAT TGGCCGTTAC GTCTGGTGTT 
AGCCGTAGAA GATCGTGTAC GGCGCGATGC TCTGTGAAGA AACCGGTGAT TCCTGAGAGT 
CCTTTCCTAG GGACTCGTGT GCGGAGATCC GGATCCGAGA CGCTTCAGTT CTGGAGATCA 
GATGGTCCTG GACGGTCTGC GAAGCTTCGA ACGGTGGTTA AATCGTCATT TTCCGCTGTT 
CCGGAGAAGC CTCTTGGTCT TTATGATCCT TCCTATGATA AAGACTCTTG TGGTGTTGGT 
TTTGTAGCAG AGTTGTCCGG CGAGACCACC CGCAAAACGG TGACTGATTC GTTGGAGATG 
TTGATACGGA TGACTCATAG AGGTGCTTGT GGTTGTGAGA GCAACACTGG TGACGGCGCT 
GGGATTCTTG TTGGTCTGCC TCATGATTTC TATGCAGAGG CTGCGACAGA GCTTGGCTTT 
GTACTTCCTT CTGCTGGAAA TTATGCTGTT GGTATGTTCT TCTTGCCAAC TGTTGAAAGC 
AGGAGAGAAG AAAGCAAGAA TGTTTTCACT AAGGTTGCTG AATCACTTGG CCACTCGGTT 
CTTGGATGGA GATTAGTGCC TACCGATAAT TCTGGACTGG GAAACTCCGC TTTGCAGACT 
GAACCGATTA TTGCGCAAGT TTTCCTTACT CCTACAACTA AATCTAAAGC TGACTTTGAG 
CAACAGATGT ATATCCTTAG AAGGGTTTCT ATGGTTGCTA TTCGAGCTGC GTTGAACCTT 
CAACATGGTG CGATGAAGGA CTTCTACATC TGTTCGTTGT CTTCTAGGAC TATCGTCTAC 
AAAGGACAAT TGAAGCCTGA TCAACTAAAG GACTATTACT ATGCGGATCT TGGGAGTGAA 
AGGTTTACGA GCTACATGGC CCTGGTTCAT TCTCGGTTCT CTACCAATAC ATTTCCCAGC 
TGGGATCGTG CTCAGCCGAT GCGTGTTTTG GGCCACAATG GGGAGATCAA TACACTCAGG 
GGAAACGTGA ACTGGATGAG GGCACGTGAG GGTCTGCTCA AGTGCAATGA ACTTGGATTA 
TCAAAGAAAG AGTTGAAGAA GCTTCTACCT ATTGTGGACG TTAGCTCTTC TGACTCAGGT 
GCTTTTGATG GTGTTCTTGA GCTTCTTGTC CGAGCTGGTA GAAGTCTTCC TGAAGCTGTT 
ATGATGATGA TCCCTGAAGC ATGGCAAAAT GACAAAAACA TTGATCCAAG CAGGAAAGAG 
TTTTATGAAT ACTTATCTGC TCTAATGGAG CCATGGGATG GACCTGCCCT CATATCATTC 
ACTGATGGTC GCTACTTGGG TGCCACATTG GACCGTAATG GACTTCGTCC TGGTAGGTTC 
TACATAACCC ACAGTGGAAG GGTTATCATG GCTAGTGAAG TTGGGGTAGT GGATGTACCA 
CCTGAAGATG TGATGAGGAA AGGAAGACTT AACCCGGGTA TGATGCTTCT TGTCGATTTT 
GAGAAGCATA TTGTTGTTGA TGACGATGCC TTGAAGCAGC AGTATTCACT GGCAAGGCCT 
TATGGTGAGT GGCTTAAAAG GCAAAAGATT GAACTTAAAG ACATCATTGA GTCAGTTCCG 
GAGGCTGAAC GGATTGCTCC TTCAATTTCT GGGGTTGTAC CGGCCTCTAA TGATGATGAC 
TCAATGGAGA GCATGGGCAT TCATGGTTTA TTGTCTCCAC TAAAAGCCTT TGGCTACACA 
GTTGAGGCCT TGGAGATGTT GCTGCTTCCG ATGGCTAAAG ATGGCTCTGA AGCACTTGGT 
TCTATGGGAA ATGATACCCC TCTAGCTGTA ATGTCAAACA GAGAAAAATT ATGCTTTGAG 
TACTTCAAGC AAATGTTTGC TCAAGTAACA AATCCTCCAA TTGATCCTAT TCGTGAGAAA 
ATTGTTACAT CAATGGAGTG CATGATCGGT CCAGAAGGCG ATCTAACCGA AACAACTGAA 
GAGCAATGTC ACCGTCTCTC CCTAAAAGGT CCTCTTTTGA AAATCGAAGA GATGGAAGCC 
ATCAAGAAAA TGAACTACAG AGGCTGGCGG ACTAAAGTTC TGGATATAAC TTATGCAAAA 
GAAAGAGGTA CAAAAGGGTT AGAGGAGACT TTGGACCGAA TTTGTGATGA AGCAAATGAA 
GCAATAAAGG AGGGCTACAC ATTACTGGTT TTGTCTGATC GAGCTTTCTC TGCAACACGT 
GTTGCTGTGA GCTCCCTCAT GGCTGTTGGT GCCGTTCATC ACCACCTGGT TAAGACACTT 
GCGCGCACCC AAGTTGGTTT GGTAGTGGAA TCTGCTGAAC CACGTGAAGT GCATCATTTC 
TGCACACTTG TCGGATTTGG AGCAGATGCT ATCTGCCCAT ATTTGGCTGT AGAGGCCGTT 
TATAGATTAC AGGTTGATGG CAAAATACCA CCCAAATCTA ATGGGGAATT CCATTCCAAA 
GAAGAGTTGG TCAAGAAGTA TTACAAGGCA AGTAACTATG GAATGATGAA GGTTCTTGCC 
AAAATGGGAA TTTCTACTCT AGCCTCATAC AAAGGTGCTC AGATATTTGA AGCTCTTGGG 
CTTTCATCTG AAGTAATTCA GAAATGTTTT GCCGGTACCC CAAGCCGAGT GGAAGGAGCA 
ACATTTGAGA TGCTTGCACG AGATGGACTT CAATTGCATG AGTTGGCCTT TCCAACCCGT 
GGCTATGCCC CAGGAAGTGC AGAAGCTTCA GCACTTACCA ACCCTGGAAA CTATCACTGG 
AGGAAAAATG GAGAGATTCA TCTCAACGAT CCTCTTGCAA TAGCCAAATT ACAAGAGGCA 
GCACGGACCA ACAGTGTGGC CGCCTACAAG GAATACTCAA AGCGTATCAA TGAATTGAAC 
AAGCAAAGTA ATTTGCGTGG GCTCATGAAG TTTAAAGACG CAGATGTGAA AATTCCACTA 
GATGAAGTCG AGCCTGCCAG TGAAATTGTT AAGCGGTTCT GCACTGGTGC CATGAGTTAT 
GGATCAATTT CTCTGGAGGC GCATACAACC CTTGCAATGG CCATGAACAA GCTTGGAGGA 
AAGTCGAATA CAGGTGAGGG AGGGGAACTG CCATCCCGTA TGGAGCCTCT TGCGGATGGT 
TCAAGGAACC CCAAAAGAAG TTCGATCAAA CAAATTGCTA GTGGAAGATT TGGAGTTTCT 
AGCTACTATC TTACAAATGC TGATGAGCTG CAGATAAAGA TGGCTCAGGG TGCCAAACCT 
GGGGAAGGTG GGGAGCTACC AGGCCACAAG GTCATAGGAG ACATTGCTAT AACCAGAAAT 
TCCACTGCTG GTGTAGGACT TATCAGTCCA CCTCCTCATC ATGATATCTA CTCTATCGAG 
GATCTTGCCC AGCTAATTCA CGATCTTAAG AATGCCAATC CTGGCGCACG TATTAGTGTT 
AAGCTTGTAT CTGAAGCTGG TGTTGGAGTT ATTGCTAGCG GTGTGGTGAA GGGGCATGCT 
GACCATGTTC TGATAGCAGG TCATGATGGA GGTACTGGTG CATCTCGCTG GACCGGTATT 
AAAAATGCTG GTCTTCCATG GGAACTGGGT CTAGCTGAGA CACACCAAAC TCTTGTTGCC 
AATGACCTTC GTGGCCGTAC TGTTCTTCAG ACAGATGGCC AGCTAAAAAC TGGCAGAGAC 
GTTGCAGTTG CTGCACTTCT TGGTGCAGAA GAATTTGGTT TTAGTACTGC ACCCCTTATC 
ACACTAGGGT GTATCATGAT GAGAAAGTGC CACAAGAACA CCTGCCCTGT GGGAATTGCT 
ACCCAAGATC CAGTTCTCCG TGAAAAATTT GCTGGGGAGC CTGAACATGT TATTAACTTC 
TTCTTTATGC TTGCTGAGGA AGTTAGGGAG ATCATGTCTG GACTTGGTTT TAGAACTGTA 
ACTGAGATGA TTGGTCGTGC GGATATGCTT GAACTTGATA GGGAAGTTGT CAAGAACAAC 
GATAAGCTGG AGAACATTGA CCTATCTCTT TTGCTCAGAC CTGCTGCTGA GATTCGTCCT 
GGGGCTGCTC AGTACTGTGT TCAGAAACAA GATCATGGTT TAGACATGGC TTTGGATCAA 
GAGCTGATTG CATTGTCTAA ATCTGCTCTG GAGAAATCTC TTCCGGTGTA CATTGAAACT 
CCTATCTGCA ACGTTAACCG TGCTGTTGGG ACAATGCTGA GCCATGAGGT AACCAAACGT 
TACCACTTGA CTGGGCTTCC AAAGGACACA ATTCATATCA AGTTCACAGG AAGTGCTGGG 
CAGAGTCTTG GAGCTTTCTT GTGTCCTGGA ATTATGCTAG AACTTGAAGG AGATAGCAAT 
GACTATGTTG GAAAAGGGCT CTCTGGAGGT AAAGTTGTTG TTTACCCTCC AAAAGGAAGC 
AGCTTTGATC CAAAAGAAAA TATCGTTATT GGTAATGTAG CACTTTACGG TGCCACTAGT 
GGTGAAGCAT ACTTCAATGG GATGGCTGCT GAAAGGTTCT CTGTTCGTAA TTCTGGTGCT 
AAAGCTGTTG TGGAAGGTCT TGGTGATCAT GGTTGTGAGT ACATGACTGG TGGTACTGTT 
GTGGTGCTTG GAAAGACTGG TAGAAACTTT GCTGCTGGTA TGAGTGGTGG TATTGCTTAT 
GTCCTTGATG TGGACGGAAA GTTTAACACC AGATGCAACC TTGAACTGGT TGATTTGGAT 
AAAGTTGAAG ATGAGGAGGA CAAAATGACG CTCAAAATGA TGATCCAGCA ACATCAAAGA 
CACACCAACA GCCAACTTGC TCAAGAAGTT CTTGCAGACT TTGAGAATTT GCTGCCCAAG 
TTTATCAAGG TTTTCCCAAG AGATTACAAA CGTGTTTTAT CAGCCATGAA ACACGAAGAG 
GTCTCCAAGC AAGCAATCGA GCGGGCTTCT GAGGAAGCTG ACGAGACTGA AGAGAAAGAA 
CTCGAGGAGA AAGATGCATT TGCAGAACTG AAGAACATGG CAGCTGCTTC GTCAAAAGAG 
GAGATGTCAG GAAACGGAGT GGCAGCTGAA GCTAGACCTT CTAAGGTAGA TAATGCTGTT 
AAAAACGGTG GTTTCATTGC TTATGAGCGT GAGGGAGTTA AGTACAGGGA TCCCAATGTT 
CGTCTTAATG ACTGGAACGA AGTCATGGAG GAATCAAAAC CTGGACCACT CCTTACAACT 
CAGTCAGCTC GTTGCATGGA TTGTGGAACT CCATTCTGCC ACCAGGAGAA CTCTGGGTGT 
CCTCTCGGTA ATAAGATCCC TGAATTCAAT GAACTTGTCT ACCAGAACAG ATGGCAAGAA 
GCCTTGAATC GTCTACTTGA GACAAACAAC TTTCCAGAAT TTACTGGGCG AGTATGCCCT 
GCACCATGTG AAGGTTCTTG TGTTCTTGGA ATAATTGAGA ACCCTGTTTC TATCAAAAGC 
ATTGAATGTG CTATTATCGA TAAAGCCTTT GAGGAAGGGT GGATGGTACC AAGGCCTCCT 
CTCAAGAGAA CAGGGAAAAA GGTTGCTATT ATCGGAAGTG GACCGGCTGG GTTAGCTGCG 
GCTGACCAGC TGAACAAAAT GGGCCATTTA GTAACCGTGT ATGAGCGCTC GGACAGAATT 
GGTGGGCTGA TGATGTATGG AGTACCAAAC ATGAAGACTG ACAAAATTGA CGTCGTTCAA 
CGGCGGGTTG ATCTTATGAC CAAAGAAGGT ATCAACTTTG TGGTCAATGC CAATATTGGA 
AAGGACCCAT CTTACTCCCT TGATGGACTT AAGGAAGAGA ATGATGCAAT CGTTCTTGCT 
GTTGGTTCCA CAAAACCAAG AGATCTTCCA GTGCCTGGTC GTGATCTATC TGGTGTTCAC 
TTTGCCATGG AGTTTCTTCA TGCAAACACC AAAAGTTTAC TTGACAGCAA TCATGAGGAT 
GGCAATTACA TTTCTGCAAA AGGGAAAAAG GTTGTTGTTA TTGGTGGAGG TGATACCGGC 
ACAGATTGCA TCGGAACATC TATCCGCCAT GGATGTACCA ACATTGTAAA CCTAGAACTT 
CTTCCTCAGC CACCCTCAAC AAGAGCTCCT GGAAACCCTT GGCCACAGTG GCCTCGTGTA 
TTCCGTATTG ACTACGGACA TCAAGAAGCT ACCACCAAAT TCGGAAAAGA CCCTAGGACC 
TATGAGGTCT TAACGAAGAG GTTCATAGGA GATGACAATG GAAACGTGAA GGGGCTTGAA 
CTTGTGCGTG TGAGTTGGGA GAAGGATGAA ACCGGGAGGT TCCAGTTCAA GGAGATCGAA 
GGCTCTGAGG AAATCATTGA AGCAGACCTC GTTTTCTTGG CCATGGGTTT CCTTGGACCT 
GAGCCAACAC TTGCTGAGAA GCTAGGTCTC GAATGTGACA ACAGGTCAAA CTTTAAGGCC 
GAGTATGGCC GGTTTTCCAC AACCGTGGAA GGTGTTTTCG CAGCAGGTGA TTGTCGGAGA 
GGCCAGTCTT TGGTTGTCTG GGCTATCTCA GAAGGACGTC AAGCTGCAGA TCAGGTAGAC 
AAATTCCTCA CCAAAACGGA TGATGACGAA GACGCCAAGC TACAACAAGA CCTTAACCAG 
ATGAAACACA ACACTATCAC AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190529.1
CDS	317..6943
Translation	MSAASSSSVLHLRTNQQLLSLRSLKNSTSVASQLAVTSGVSRRRSCTARCSVKKPVIPESPFLGTRVRRSGSETLQFWRSDGPGRSAKLRTVVKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTRKTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEAATELGFVLPSAGNYAVGMFFLPTVESRREESKNVFTKVAESLGHSVLGWRLVPTDNSGLGNSALQTEPIIAQVFLTPTTKSKADFEQQMYILRRVSMVAIRAALNLQHGAMKDFYICSLSSRTIVYKGQLKPDQLKDYYYADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKEFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSLARPYGEWLKRQKIELKDIIESVPEAERIAPSISGVVPASNDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGSEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLKIEEMEAIKKMNYRGWRTKVLDITYAKERGTKGLEETLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLMAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVYRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIQKCFAGTPSRVEGATFEMLARDGLQLHELAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQSNLRGLMKFKDADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLADGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTVTEMIGRADMLELDREVVKNNDKLENIDLSLLLRPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSLPVYIETPICNVNRAVGTMLSHEVTKRYHLTGLPKDTIHIKFTGSAGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGAKAVVEGLGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFNTRCNLELVDLDKVEDEEDKMTLKMMIQQHQRHTNSQLAQEVLADFENLLPKFIKVFPRDYKRVLSAMKHEEVSKQAIERASEEADETEEKELEEKDAFAELKNMAAASSKEEMSGNGVAAEARPSKVDNAVKNGGFIAYEREGVKYRDPNVRLNDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFEEGWMVPRPPLKRTGKKVAIIGSGPAGLAAADQLNKMGHLVTVYERSDRIGGLMMYGVPNMKTDKIDVVQRRVDLMTKEGINFVVNANIGKDPSYSLDGLKEENDAIVLAVGSTKPRDLPVPGRDLSGVHFAMEFLHANTKSLLDSNHEDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQEATTKFGKDPRTYEVLTKRFIGDDNGNVKGLELVRVSWEKDETGRFQFKEIEGSEEIIEADLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWAISEGRQAADQVDKFLTKTDDDEDAKLQQDLNQMKHNTITN

Target ORF information:

RefSeq Version	NM_001203600.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NADH-dependent glutamate synthase 1 (GLT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203600.1

ORF Insert Sequence:

ATGTCGGCAG CTTCTTCTAG CTCCGTTCTT CACCTCCGAA CCAACCAACA ACTGTTATCA 
CTCCGATCTC TCAAAAATTC CACCTCCGTT GCTTCTCAAT TGGCCGTTAC GTCTGGTGTT 
AGCCGTAGAA GATCGTGTAC GGCGCGATGC TCTGTGAAGA AACCGGTGAT TCCTGAGAGT 
CCTTTCCTAG GGACTCGTGT GCGGAGATCC GGATCCGAGA CGCTTCAGTT CTGGAGATCA 
GATGGTCCTG GACGGTCTGC GAAGCTTCGA ACGGTGGTTA AATCGTCATT TTCCGCTGTT 
CCGGAGAAGC CTCTTGGTCT TTATGATCCT TCCTATGATA AAGACTCTTG TGGTGTTGGT 
TTTGTAGCAG AGTTGTCCGG CGAGACCACC CGCAAAACGG TGACTGATTC GTTGGAGATG 
TTGATACGGA TGACTCATAG AGGTGCTTGT GGTTGTGAGA GCAACACTGG TGACGGCGCT 
GGGATTCTTG TTGGTCTGCC TCATGATTTC TATGCAGAGG CTGCGACAGA GCTTGGCTTT 
GTACTTCCTT CTGCTGGAAA TTATGCTGTT GGTATGTTCT TCTTGCCAAC TGTTGAAAGC 
AGGAGAGAAG AAAGCAAGAA TGTTTTCACT AAGGTTGCTG AATCACTTGG CCACTCGGTT 
CTTGGATGGA GATTAGTGCC TACCGATAAT TCTGGACTGG GAAACTCCGC TTTGCAGACT 
GAACCGATTA TTGCGCAAGT TTTCCTTACT CCTACAACTA AATCTAAAGC TGACTTTGAG 
CAACAGATGT ATATCCTTAG AAGGGTTTCT ATGGTTGCTA TTCGAGCTGC GTTGAACCTT 
CAACATGGTG CGATGAAGGA CTTCTACATC TGTTCGTTGT CTTCTAGGAC TATCGTCTAC 
AAAGGACAAT TGAAGCCTGA TCAACTAAAG GACTATTACT ATGCGGATCT TGGGAGTGAA 
AGGTTTACGA GCTACATGGC CCTGGTTCAT TCTCGGTTCT CTACCAATAC ATTTCCCAGC 
TGGGATCGTG CTCAGCCGAT GCGTGTTTTG GGCCACAATG GGGAGATCAA TACACTCAGG 
GGAAACGTGA ACTGGATGAG GGCACGTGAG GGTCTGCTCA AGTGCAATGA ACTTGGATTA 
TCAAAGAAAG AGTTGAAGAA GCTTCTACCT ATTGTGGACG TTAGCTCTTC TGACTCAGGT 
GCTTTTGATG GTGTTCTTGA GCTTCTTGTC CGAGCTGGTA GAAGTCTTCC TGAAGCTGTT 
ATGATGATGA TCCCTGAAGC ATGGCAAAAT GACAAAAACA TTGATCCAAG CAGGAAAGAG 
TTTTATGAAT ACTTATCTGC TCTAATGGAG CCATGGGATG GACCTGCCCT CATATCATTC 
ACTGATGGTC GCTACTTGGG TGCCACATTG GACCGTAATG GACTTCGTCC TGGTAGGTTC 
TACATAACCC ACAGTGGAAG GGTTATCATG GCTAGTGAAG TTGGGGTAGT GGATGTACCA 
CCTGAAGATG TGATGAGGAA AGGAAGACTT AACCCGGGTA TGATGCTTCT TGTCGATTTT 
GAGAAGCATA TTGTTGTTGA TGACGATGCC TTGAAGCAGC AGTATTCACT GGCAAGGCCT 
TATGGTGAGT GGCTTAAAAG GCAAAAGATT GAACTTAAAG ACATCATTGA GTCAGTTCCG 
GAGGCTGAAC GGATTGCTCC TTCAATTTCT GGGGTTGTAC CGGCCTCTAA TGATGATGAC 
TCAATGGAGA GCATGGGCAT TCATGGTTTA TTGTCTCCAC TAAAAGCCTT TGGCTACACA 
GTTGAGGCCT TGGAGATGTT GCTGCTTCCG ATGGCTAAAG ATGGCTCTGA AGCACTTGGT 
TCTATGGGAA ATGATACCCC TCTAGCTGTA ATGTCAAACA GAGAAAAATT ATGCTTTGAG 
TACTTCAAGC AAATGTTTGC TCAAGTAACA AATCCTCCAA TTGATCCTAT TCGTGAGAAA 
ATTGTTACAT CAATGGAGTG CATGATCGGT CCAGAAGGCG ATCTAACCGA AACAACTGAA 
GAGCAATGTC ACCGTCTCTC CCTAAAAGGT CCTCTTTTGA AAATCGAAGA GATGGAAGCC 
ATCAAGAAAA TGAACTACAG AGGCTGGCGG ACTAAAGTTC TGGATATAAC TTATGCAAAA 
GAAAGAGGTA CAAAAGGGTT AGAGGAGACT TTGGACCGAA TTTGTGATGA AGCAAATGAA 
GCAATAAAGG AGGGCTACAC ATTACTGGTT TTGTCTGATC GAGCTTTCTC TGCAACACGT 
GTTGCTGTGA GCTCCCTCAT GGCTGTTGGT GCCGTTCATC ACCACCTGGT TAAGACACTT 
GCGCGCACCC AAGTTGGTTT GGTAGTGGAA TCTGCTGAAC CACGTGAAGT GCATCATTTC 
TGCACACTTG TCGGATTTGG AGCAGATGCT ATCTGCCCAT ATTTGGCTGT AGAGGCCGTT 
TATAGATTAC AGGTTGATGG CAAAATACCA CCCAAATCTA ATGGGGAATT CCATTCCAAA 
GAAGAGTTGG TCAAGAAGTA TTACAAGGCA AGTAACTATG GAATGATGAA GGTTCTTGCC 
AAAATGGGAA TTTCTACTCT AGCCTCATAC AAAGGTGCTC AGATATTTGA AGCTCTTGGG 
CTTTCATCTG AAGTAATTCA GAAATGTTTT GCCGGTACCC CAAGCCGAGT GGAAGGAGCA 
ACATTTGAGA TGCTTGCACG AGATGGACTT CAATTGCATG AGTTGGCCTT TCCAACCCGT 
GGCTATGCCC CAGGAAGTGC AGAAGCTTCA GCACTTACCA ACCCTGGAAA CTATCACTGG 
AGGAAAAATG GAGAGATTCA TCTCAACGAT CCTCTTGCAA TAGCCAAATT ACAAGAGGCA 
GCACGGACCA ACAGTGTGGC CGCCTACAAG GAATACTCAA AGCGTATCAA TGAATTGAAC 
AAGCAAAGTA ATTTGCGTGG GCTCATGAAG TTTAAAGACG CAGATGTGAA AATTCCACTA 
GATGAAGTCG AGCCTGCCAG TGAAATTGTT AAGCGGTTCT GCACTGGTGC CATGAGTTAT 
GGATCAATTT CTCTGGAGGC GCATACAACC CTTGCAATGG CCATGAACAA GCTTGGAGGA 
AAGTCGAATA CAGGTGAGGG AGGGGAACTG CCATCCCGTA TGGAGCCTCT TGCGGATGGT 
TCAAGGAACC CCAAAAGAAG TTCGATCAAA CAAATTGCTA GTGGAAGATT TGGAGTTTCT 
AGCTACTATC TTACAAATGC TGATGAGCTG CAGATAAAGA TGGCTCAGGG TGCCAAACCT 
GGGGAAGGTG GGGAGCTACC AGGCCACAAG GTCATAGGAG ACATTGCTAT AACCAGAAAT 
TCCACTGCTG GTGTAGGACT TATCAGTCCA CCTCCTCATC ATGATATCTA CTCTATCGAG 
GATCTTGCCC AGCTAATTCA CGATCTTAAG AATGCCAATC CTGGCGCACG TATTAGTGTT 
AAGCTTGTAT CTGAAGCTGG TGTTGGAGTT ATTGCTAGCG GTGTGGTGAA GGGGCATGCT 
GACCATGTTC TGATAGCAGG TCATGATGGA GGTACTGGTG CATCTCGCTG GACCGGTATT 
AAAAATGCTG GTCTTCCATG GGAACTGGGT CTAGCTGAGA CACACCAAAC TCTTGTTGCC 
AATGACCTTC GTGGCCGTAC TGTTCTTCAG ACAGATGGCC AGCTAAAAAC TGGCAGAGAC 
GTTGCAGTTG CTGCACTTCT TGGTGCAGAA GAATTTGGTT TTAGTACTGC ACCCCTTATC 
ACACTAGGGT GTATCATGAT GAGAAAGTGC CACAAGAACA CCTGCCCTGT GGGAATTGCT 
ACCCAAGATC CAGTTCTCCG TGAAAAATTT GCTGGGGAGC CTGAACATGT TATTAACTTC 
TTCTTTATGC TTGCTGAGGA AGTTAGGGAG ATCATGTCTG GACTTGGTTT TAGAACTGTA 
ACTGAGATGA TTGGTCGTGC GGATATGCTT GAACTTGATA GGGAAGTTGT CAAGAACAAC 
GATAAGCTGG AGAACATTGA CCTATCTCTT TTGCTCAGAC CTGCTGCTGA GATTCGTCCT 
GGGGCTGCTC AGTACTGTGT TCAGAAACAA GATCATGGTT TAGACATGGC TTTGGATCAA 
GAGCTGATTG CATTGTCTAA ATCTGCTCTG GAGAAATCTC TTCCGGTGTA CATTGAAACT 
CCTATCTGCA ACGTTAACCG TGCTGTTGGG ACAATGCTGA GCCATGAGGT AACCAAACGT 
TACCACTTGA CTGGGCTTCC AAAGGACACA ATTCATATCA AGTTCACAGG AAGTGCTGGG 
CAGAGTCTTG GAGCTTTCTT GTGTCCTGGA ATTATGCTAG AACTTGAAGG AGATAGCAAT 
GACTATGTTG GAAAAGGGCT CTCTGGAGGT AAAGTTGTTG TTTACCCTCC AAAAGGAAGC 
AGCTTTGATC CAAAAGAAAA TATCGTTATT GGTAATGTAG CACTTTACGG TGCCACTAGT 
GGTGAAGCAT ACTTCAATGG GATGGCTGCT GAAAGGTTCT CTGTTCGTAA TTCTGGTGCT 
AAAGCTGTTG TGGAAGGTCT TGGTGATCAT GGTTGTGAGT ACATGACTGG TGGTACTGTT 
GTGGTGCTTG GAAAGACTGG TAGAAACTTT GCTGCTGGTA TGAGTGGTGG TATTGCTTAT 
GTCCTTGATG TGGACGGAAA GTTTAACACC AGATGCAACC TTGAACTGGT TGATTTGGAT 
AAAGTTGAAG ATGAGGAGGA CAAAATGACG CTCAAAATGA TGATCCAGCA ACATCAAAGA 
CACACCAACA GCCAACTTGC TCAAGAAGTT CTTGCAGACT TTGAGAATTT GCTGCCCAAG 
TTTATCAAGG TTTTCCCAAG AGATTACAAA CGTGTTTTAT CAGCCATGAA ACACGAAGAG 
GTCTCCAAGC AAGCAATCGA GCGGGCTTCT GAGGAAGCTG ACGAGACTGA AGAGAAAGAA 
CTCGAGGAGA AAGATGCATT TGCAGAACTG AAGAACATGG CAGCTGCTTC GTCAAAAGAG 
GAGATGTCAG GAAACGGAGT GGCAGCTGAA GCTAGACCTT CTAAGGTAGA TAATGCTGTT 
AAAAACGGTG GTTTCATTGC TTATGAGCGT GAGGGAGTTA AGTACAGGGA TCCCAATGTT 
CGTCTTAATG ACTGGAACGA AGTCATGGAG GAATCAAAAC CTGGACCACT CCTTACAACT 
CAGTCAGCTC GTTGCATGGA TTGTGGAACT CCATTCTGCC ACCAGGAGAA CTCTGGGTGT 
CCTCTCGGTA ATAAGATCCC TGAATTCAAT GAACTTGTCT ACCAGAACAG ATGGCAAGAA 
GCCTTGAATC GTCTACTTGA GACAAACAAC TTTCCAGAAT TTACTGGGCG AGTATGCCCT 
GCACCATGTG AAGGTTCTTG TGTTCTTGGA ATAATTGAGA ACCCTGTTTC TATCAAAAGC 
ATTGAATGTG CTATTATCGA TAAAGCCTTT GAGGAAGGGT GGATGGTACC AAGGCCTCCT 
CTCAAGAGAA CAGGGAAAAA GGTTGCTATT ATCGGAAGTG GACCGGCTGG GTTAGCTGCG 
GCTGACCAGC TGAACAAAAT GGGCCATTTA GTAACCGTGT ATGAGCGCTC GGACAGAATT 
GGTGGGCTGA TGATGTATGG AGTACCAAAC ATGAAGACTG ACAAAATTGA CGTCGTTCAA 
CGGCGGGTTG ATCTTATGAC CAAAGAAGGT ATCAACTTTG TGGTCAATGC CAATATTGGA 
AAGGACCCAT CTTACTCCCT TGATGGACTT AAGGAAGAGA ATGATGCAAT CGTTCTTGCT 
GTTGGTTCCA CAAAACCAAG AGATCTTCCA GTGCCTGGTC GTGATCTATC TGGTGTTCAC 
TTTGCCATGG AGTTTCTTCA TGCAAACACC AAAAGTTTAC TTGACAGCAA TCATGAGGAT 
GGCAATTACA TTTCTGCAAA AGGGAAAAAG GTTGTTGTTA TTGGTGGAGG TGATACCGGC 
ACAGATTGCA TCGGAACATC TATCCGCCAT GGATGTACCA ACATTGTAAA CCTAGAACTT 
CTTCCTCAGC CACCCTCAAC AAGAGCTCCT GGAAACCCTT GGCCACAGTG GCCTCGTGTA 
TTCCGTATTG ACTACGGACA TCAAGAAGCT ACCACCAAAT TCGGAAAAGA CCCTAGGACC 
TATGAGGTCT TAACGAAGAG GTTCATAGGA GATGACAATG GAAACGTGAA GGGGCTTGAA 
CTTGTGCGTG TGAGTTGGGA GAAGGATGAA ACCGGGAGGT TCCAGTTCAA GGAGATCGAA 
GGCTCTGAGG AAATCATTGA AGCAGACCTC GTTTTCTTGG CCATGGGTTT CCTTGGACCT 
GAGCCAACAC TTGCTGAGAA GCTAGGTCTC GAATGTGACA ACAGGTCAAA CTTTAAGGCC 
GAGTATGGCC GGTTTTCCAC AACCGTGGAA GGTGTTTTCG CAGCAGGTGA TTGTCGGAGA 
GGCCAGTCTT TGGTTGTCTG GGCTATCTCA GAAGGACGTC AAGCTGCAGA TCAGGTAGAC 
AAATTCCTCA CCAAAACGGA TGATGACGAA GACGCCAAGC TACAACAAGA CCTTAACCAG 
ATGAAACACA ACACTATCAC AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLT1 ( NM_001203601.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190530.1 Arabidopsis thaliana NADH-dependent glutamate synthase 1 (GLT1), mRNA.

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CloneID	OAb21327
Clone ID Related Accession (Same CDS sequence)	NM_001203600.1 , NM_001203601.1 , NM_124725.5
Accession Version	NM_001203601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NADH-dependent glutamate synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGTCGGCAG CTTCTTCTAG CTCCGTTCTT CACCTCCGAA CCAACCAACA ACTGTTATCA 
CTCCGATCTC TCAAAAATTC CACCTCCGTT GCTTCTCAAT TGGCCGTTAC GTCTGGTGTT 
AGCCGTAGAA GATCGTGTAC GGCGCGATGC TCTGTGAAGA AACCGGTGAT TCCTGAGAGT 
CCTTTCCTAG GGACTCGTGT GCGGAGATCC GGATCCGAGA CGCTTCAGTT CTGGAGATCA 
GATGGTCCTG GACGGTCTGC GAAGCTTCGA ACGGTGGTTA AATCGTCATT TTCCGCTGTT 
CCGGAGAAGC CTCTTGGTCT TTATGATCCT TCCTATGATA AAGACTCTTG TGGTGTTGGT 
TTTGTAGCAG AGTTGTCCGG CGAGACCACC CGCAAAACGG TGACTGATTC GTTGGAGATG 
TTGATACGGA TGACTCATAG AGGTGCTTGT GGTTGTGAGA GCAACACTGG TGACGGCGCT 
GGGATTCTTG TTGGTCTGCC TCATGATTTC TATGCAGAGG CTGCGACAGA GCTTGGCTTT 
GTACTTCCTT CTGCTGGAAA TTATGCTGTT GGTATGTTCT TCTTGCCAAC TGTTGAAAGC 
AGGAGAGAAG AAAGCAAGAA TGTTTTCACT AAGGTTGCTG AATCACTTGG CCACTCGGTT 
CTTGGATGGA GATTAGTGCC TACCGATAAT TCTGGACTGG GAAACTCCGC TTTGCAGACT 
GAACCGATTA TTGCGCAAGT TTTCCTTACT CCTACAACTA AATCTAAAGC TGACTTTGAG 
CAACAGATGT ATATCCTTAG AAGGGTTTCT ATGGTTGCTA TTCGAGCTGC GTTGAACCTT 
CAACATGGTG CGATGAAGGA CTTCTACATC TGTTCGTTGT CTTCTAGGAC TATCGTCTAC 
AAAGGACAAT TGAAGCCTGA TCAACTAAAG GACTATTACT ATGCGGATCT TGGGAGTGAA 
AGGTTTACGA GCTACATGGC CCTGGTTCAT TCTCGGTTCT CTACCAATAC ATTTCCCAGC 
TGGGATCGTG CTCAGCCGAT GCGTGTTTTG GGCCACAATG GGGAGATCAA TACACTCAGG 
GGAAACGTGA ACTGGATGAG GGCACGTGAG GGTCTGCTCA AGTGCAATGA ACTTGGATTA 
TCAAAGAAAG AGTTGAAGAA GCTTCTACCT ATTGTGGACG TTAGCTCTTC TGACTCAGGT 
GCTTTTGATG GTGTTCTTGA GCTTCTTGTC CGAGCTGGTA GAAGTCTTCC TGAAGCTGTT 
ATGATGATGA TCCCTGAAGC ATGGCAAAAT GACAAAAACA TTGATCCAAG CAGGAAAGAG 
TTTTATGAAT ACTTATCTGC TCTAATGGAG CCATGGGATG GACCTGCCCT CATATCATTC 
ACTGATGGTC GCTACTTGGG TGCCACATTG GACCGTAATG GACTTCGTCC TGGTAGGTTC 
TACATAACCC ACAGTGGAAG GGTTATCATG GCTAGTGAAG TTGGGGTAGT GGATGTACCA 
CCTGAAGATG TGATGAGGAA AGGAAGACTT AACCCGGGTA TGATGCTTCT TGTCGATTTT 
GAGAAGCATA TTGTTGTTGA TGACGATGCC TTGAAGCAGC AGTATTCACT GGCAAGGCCT 
TATGGTGAGT GGCTTAAAAG GCAAAAGATT GAACTTAAAG ACATCATTGA GTCAGTTCCG 
GAGGCTGAAC GGATTGCTCC TTCAATTTCT GGGGTTGTAC CGGCCTCTAA TGATGATGAC 
TCAATGGAGA GCATGGGCAT TCATGGTTTA TTGTCTCCAC TAAAAGCCTT TGGCTACACA 
GTTGAGGCCT TGGAGATGTT GCTGCTTCCG ATGGCTAAAG ATGGCTCTGA AGCACTTGGT 
TCTATGGGAA ATGATACCCC TCTAGCTGTA ATGTCAAACA GAGAAAAATT ATGCTTTGAG 
TACTTCAAGC AAATGTTTGC TCAAGTAACA AATCCTCCAA TTGATCCTAT TCGTGAGAAA 
ATTGTTACAT CAATGGAGTG CATGATCGGT CCAGAAGGCG ATCTAACCGA AACAACTGAA 
GAGCAATGTC ACCGTCTCTC CCTAAAAGGT CCTCTTTTGA AAATCGAAGA GATGGAAGCC 
ATCAAGAAAA TGAACTACAG AGGCTGGCGG ACTAAAGTTC TGGATATAAC TTATGCAAAA 
GAAAGAGGTA CAAAAGGGTT AGAGGAGACT TTGGACCGAA TTTGTGATGA AGCAAATGAA 
GCAATAAAGG AGGGCTACAC ATTACTGGTT TTGTCTGATC GAGCTTTCTC TGCAACACGT 
GTTGCTGTGA GCTCCCTCAT GGCTGTTGGT GCCGTTCATC ACCACCTGGT TAAGACACTT 
GCGCGCACCC AAGTTGGTTT GGTAGTGGAA TCTGCTGAAC CACGTGAAGT GCATCATTTC 
TGCACACTTG TCGGATTTGG AGCAGATGCT ATCTGCCCAT ATTTGGCTGT AGAGGCCGTT 
TATAGATTAC AGGTTGATGG CAAAATACCA CCCAAATCTA ATGGGGAATT CCATTCCAAA 
GAAGAGTTGG TCAAGAAGTA TTACAAGGCA AGTAACTATG GAATGATGAA GGTTCTTGCC 
AAAATGGGAA TTTCTACTCT AGCCTCATAC AAAGGTGCTC AGATATTTGA AGCTCTTGGG 
CTTTCATCTG AAGTAATTCA GAAATGTTTT GCCGGTACCC CAAGCCGAGT GGAAGGAGCA 
ACATTTGAGA TGCTTGCACG AGATGGACTT CAATTGCATG AGTTGGCCTT TCCAACCCGT 
GGCTATGCCC CAGGAAGTGC AGAAGCTTCA GCACTTACCA ACCCTGGAAA CTATCACTGG 
AGGAAAAATG GAGAGATTCA TCTCAACGAT CCTCTTGCAA TAGCCAAATT ACAAGAGGCA 
GCACGGACCA ACAGTGTGGC CGCCTACAAG GAATACTCAA AGCGTATCAA TGAATTGAAC 
AAGCAAAGTA ATTTGCGTGG GCTCATGAAG TTTAAAGACG CAGATGTGAA AATTCCACTA 
GATGAAGTCG AGCCTGCCAG TGAAATTGTT AAGCGGTTCT GCACTGGTGC CATGAGTTAT 
GGATCAATTT CTCTGGAGGC GCATACAACC CTTGCAATGG CCATGAACAA GCTTGGAGGA 
AAGTCGAATA CAGGTGAGGG AGGGGAACTG CCATCCCGTA TGGAGCCTCT TGCGGATGGT 
TCAAGGAACC CCAAAAGAAG TTCGATCAAA CAAATTGCTA GTGGAAGATT TGGAGTTTCT 
AGCTACTATC TTACAAATGC TGATGAGCTG CAGATAAAGA TGGCTCAGGG TGCCAAACCT 
GGGGAAGGTG GGGAGCTACC AGGCCACAAG GTCATAGGAG ACATTGCTAT AACCAGAAAT 
TCCACTGCTG GTGTAGGACT TATCAGTCCA CCTCCTCATC ATGATATCTA CTCTATCGAG 
GATCTTGCCC AGCTAATTCA CGATCTTAAG AATGCCAATC CTGGCGCACG TATTAGTGTT 
AAGCTTGTAT CTGAAGCTGG TGTTGGAGTT ATTGCTAGCG GTGTGGTGAA GGGGCATGCT 
GACCATGTTC TGATAGCAGG TCATGATGGA GGTACTGGTG CATCTCGCTG GACCGGTATT 
AAAAATGCTG GTCTTCCATG GGAACTGGGT CTAGCTGAGA CACACCAAAC TCTTGTTGCC 
AATGACCTTC GTGGCCGTAC TGTTCTTCAG ACAGATGGCC AGCTAAAAAC TGGCAGAGAC 
GTTGCAGTTG CTGCACTTCT TGGTGCAGAA GAATTTGGTT TTAGTACTGC ACCCCTTATC 
ACACTAGGGT GTATCATGAT GAGAAAGTGC CACAAGAACA CCTGCCCTGT GGGAATTGCT 
ACCCAAGATC CAGTTCTCCG TGAAAAATTT GCTGGGGAGC CTGAACATGT TATTAACTTC 
TTCTTTATGC TTGCTGAGGA AGTTAGGGAG ATCATGTCTG GACTTGGTTT TAGAACTGTA 
ACTGAGATGA TTGGTCGTGC GGATATGCTT GAACTTGATA GGGAAGTTGT CAAGAACAAC 
GATAAGCTGG AGAACATTGA CCTATCTCTT TTGCTCAGAC CTGCTGCTGA GATTCGTCCT 
GGGGCTGCTC AGTACTGTGT TCAGAAACAA GATCATGGTT TAGACATGGC TTTGGATCAA 
GAGCTGATTG CATTGTCTAA ATCTGCTCTG GAGAAATCTC TTCCGGTGTA CATTGAAACT 
CCTATCTGCA ACGTTAACCG TGCTGTTGGG ACAATGCTGA GCCATGAGGT AACCAAACGT 
TACCACTTGA CTGGGCTTCC AAAGGACACA ATTCATATCA AGTTCACAGG AAGTGCTGGG 
CAGAGTCTTG GAGCTTTCTT GTGTCCTGGA ATTATGCTAG AACTTGAAGG AGATAGCAAT 
GACTATGTTG GAAAAGGGCT CTCTGGAGGT AAAGTTGTTG TTTACCCTCC AAAAGGAAGC 
AGCTTTGATC CAAAAGAAAA TATCGTTATT GGTAATGTAG CACTTTACGG TGCCACTAGT 
GGTGAAGCAT ACTTCAATGG GATGGCTGCT GAAAGGTTCT CTGTTCGTAA TTCTGGTGCT 
AAAGCTGTTG TGGAAGGTCT TGGTGATCAT GGTTGTGAGT ACATGACTGG TGGTACTGTT 
GTGGTGCTTG GAAAGACTGG TAGAAACTTT GCTGCTGGTA TGAGTGGTGG TATTGCTTAT 
GTCCTTGATG TGGACGGAAA GTTTAACACC AGATGCAACC TTGAACTGGT TGATTTGGAT 
AAAGTTGAAG ATGAGGAGGA CAAAATGACG CTCAAAATGA TGATCCAGCA ACATCAAAGA 
CACACCAACA GCCAACTTGC TCAAGAAGTT CTTGCAGACT TTGAGAATTT GCTGCCCAAG 
TTTATCAAGG TTTTCCCAAG AGATTACAAA CGTGTTTTAT CAGCCATGAA ACACGAAGAG 
GTCTCCAAGC AAGCAATCGA GCGGGCTTCT GAGGAAGCTG ACGAGACTGA AGAGAAAGAA 
CTCGAGGAGA AAGATGCATT TGCAGAACTG AAGAACATGG CAGCTGCTTC GTCAAAAGAG 
GAGATGTCAG GAAACGGAGT GGCAGCTGAA GCTAGACCTT CTAAGGTAGA TAATGCTGTT 
AAAAACGGTG GTTTCATTGC TTATGAGCGT GAGGGAGTTA AGTACAGGGA TCCCAATGTT 
CGTCTTAATG ACTGGAACGA AGTCATGGAG GAATCAAAAC CTGGACCACT CCTTACAACT 
CAGTCAGCTC GTTGCATGGA TTGTGGAACT CCATTCTGCC ACCAGGAGAA CTCTGGGTGT 
CCTCTCGGTA ATAAGATCCC TGAATTCAAT GAACTTGTCT ACCAGAACAG ATGGCAAGAA 
GCCTTGAATC GTCTACTTGA GACAAACAAC TTTCCAGAAT TTACTGGGCG AGTATGCCCT 
GCACCATGTG AAGGTTCTTG TGTTCTTGGA ATAATTGAGA ACCCTGTTTC TATCAAAAGC 
ATTGAATGTG CTATTATCGA TAAAGCCTTT GAGGAAGGGT GGATGGTACC AAGGCCTCCT 
CTCAAGAGAA CAGGGAAAAA GGTTGCTATT ATCGGAAGTG GACCGGCTGG GTTAGCTGCG 
GCTGACCAGC TGAACAAAAT GGGCCATTTA GTAACCGTGT ATGAGCGCTC GGACAGAATT 
GGTGGGCTGA TGATGTATGG AGTACCAAAC ATGAAGACTG ACAAAATTGA CGTCGTTCAA 
CGGCGGGTTG ATCTTATGAC CAAAGAAGGT ATCAACTTTG TGGTCAATGC CAATATTGGA 
AAGGACCCAT CTTACTCCCT TGATGGACTT AAGGAAGAGA ATGATGCAAT CGTTCTTGCT 
GTTGGTTCCA CAAAACCAAG AGATCTTCCA GTGCCTGGTC GTGATCTATC TGGTGTTCAC 
TTTGCCATGG AGTTTCTTCA TGCAAACACC AAAAGTTTAC TTGACAGCAA TCATGAGGAT 
GGCAATTACA TTTCTGCAAA AGGGAAAAAG GTTGTTGTTA TTGGTGGAGG TGATACCGGC 
ACAGATTGCA TCGGAACATC TATCCGCCAT GGATGTACCA ACATTGTAAA CCTAGAACTT 
CTTCCTCAGC CACCCTCAAC AAGAGCTCCT GGAAACCCTT GGCCACAGTG GCCTCGTGTA 
TTCCGTATTG ACTACGGACA TCAAGAAGCT ACCACCAAAT TCGGAAAAGA CCCTAGGACC 
TATGAGGTCT TAACGAAGAG GTTCATAGGA GATGACAATG GAAACGTGAA GGGGCTTGAA 
CTTGTGCGTG TGAGTTGGGA GAAGGATGAA ACCGGGAGGT TCCAGTTCAA GGAGATCGAA 
GGCTCTGAGG AAATCATTGA AGCAGACCTC GTTTTCTTGG CCATGGGTTT CCTTGGACCT 
GAGCCAACAC TTGCTGAGAA GCTAGGTCTC GAATGTGACA ACAGGTCAAA CTTTAAGGCC 
GAGTATGGCC GGTTTTCCAC AACCGTGGAA GGTGTTTTCG CAGCAGGTGA TTGTCGGAGA 
GGCCAGTCTT TGGTTGTCTG GGCTATCTCA GAAGGACGTC AAGCTGCAGA TCAGGTAGAC 
AAATTCCTCA CCAAAACGGA TGATGACGAA GACGCCAAGC TACAACAAGA CCTTAACCAG 
ATGAAACACA ACACTATCAC AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190530.1
CDS	317..6943
Translation	MSAASSSSVLHLRTNQQLLSLRSLKNSTSVASQLAVTSGVSRRRSCTARCSVKKPVIPESPFLGTRVRRSGSETLQFWRSDGPGRSAKLRTVVKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTRKTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEAATELGFVLPSAGNYAVGMFFLPTVESRREESKNVFTKVAESLGHSVLGWRLVPTDNSGLGNSALQTEPIIAQVFLTPTTKSKADFEQQMYILRRVSMVAIRAALNLQHGAMKDFYICSLSSRTIVYKGQLKPDQLKDYYYADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKEFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSLARPYGEWLKRQKIELKDIIESVPEAERIAPSISGVVPASNDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGSEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLKIEEMEAIKKMNYRGWRTKVLDITYAKERGTKGLEETLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLMAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVYRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIQKCFAGTPSRVEGATFEMLARDGLQLHELAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQSNLRGLMKFKDADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLADGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTVTEMIGRADMLELDREVVKNNDKLENIDLSLLLRPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSLPVYIETPICNVNRAVGTMLSHEVTKRYHLTGLPKDTIHIKFTGSAGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGAKAVVEGLGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFNTRCNLELVDLDKVEDEEDKMTLKMMIQQHQRHTNSQLAQEVLADFENLLPKFIKVFPRDYKRVLSAMKHEEVSKQAIERASEEADETEEKELEEKDAFAELKNMAAASSKEEMSGNGVAAEARPSKVDNAVKNGGFIAYEREGVKYRDPNVRLNDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFEEGWMVPRPPLKRTGKKVAIIGSGPAGLAAADQLNKMGHLVTVYERSDRIGGLMMYGVPNMKTDKIDVVQRRVDLMTKEGINFVVNANIGKDPSYSLDGLKEENDAIVLAVGSTKPRDLPVPGRDLSGVHFAMEFLHANTKSLLDSNHEDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQEATTKFGKDPRTYEVLTKRFIGDDNGNVKGLELVRVSWEKDETGRFQFKEIEGSEEIIEADLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWAISEGRQAADQVDKFLTKTDDDEDAKLQQDLNQMKHNTITN

Target ORF information:

RefSeq Version	NM_001203601.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NADH-dependent glutamate synthase 1 (GLT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203601.1

ORF Insert Sequence:

ATGTCGGCAG CTTCTTCTAG CTCCGTTCTT CACCTCCGAA CCAACCAACA ACTGTTATCA 
CTCCGATCTC TCAAAAATTC CACCTCCGTT GCTTCTCAAT TGGCCGTTAC GTCTGGTGTT 
AGCCGTAGAA GATCGTGTAC GGCGCGATGC TCTGTGAAGA AACCGGTGAT TCCTGAGAGT 
CCTTTCCTAG GGACTCGTGT GCGGAGATCC GGATCCGAGA CGCTTCAGTT CTGGAGATCA 
GATGGTCCTG GACGGTCTGC GAAGCTTCGA ACGGTGGTTA AATCGTCATT TTCCGCTGTT 
CCGGAGAAGC CTCTTGGTCT TTATGATCCT TCCTATGATA AAGACTCTTG TGGTGTTGGT 
TTTGTAGCAG AGTTGTCCGG CGAGACCACC CGCAAAACGG TGACTGATTC GTTGGAGATG 
TTGATACGGA TGACTCATAG AGGTGCTTGT GGTTGTGAGA GCAACACTGG TGACGGCGCT 
GGGATTCTTG TTGGTCTGCC TCATGATTTC TATGCAGAGG CTGCGACAGA GCTTGGCTTT 
GTACTTCCTT CTGCTGGAAA TTATGCTGTT GGTATGTTCT TCTTGCCAAC TGTTGAAAGC 
AGGAGAGAAG AAAGCAAGAA TGTTTTCACT AAGGTTGCTG AATCACTTGG CCACTCGGTT 
CTTGGATGGA GATTAGTGCC TACCGATAAT TCTGGACTGG GAAACTCCGC TTTGCAGACT 
GAACCGATTA TTGCGCAAGT TTTCCTTACT CCTACAACTA AATCTAAAGC TGACTTTGAG 
CAACAGATGT ATATCCTTAG AAGGGTTTCT ATGGTTGCTA TTCGAGCTGC GTTGAACCTT 
CAACATGGTG CGATGAAGGA CTTCTACATC TGTTCGTTGT CTTCTAGGAC TATCGTCTAC 
AAAGGACAAT TGAAGCCTGA TCAACTAAAG GACTATTACT ATGCGGATCT TGGGAGTGAA 
AGGTTTACGA GCTACATGGC CCTGGTTCAT TCTCGGTTCT CTACCAATAC ATTTCCCAGC 
TGGGATCGTG CTCAGCCGAT GCGTGTTTTG GGCCACAATG GGGAGATCAA TACACTCAGG 
GGAAACGTGA ACTGGATGAG GGCACGTGAG GGTCTGCTCA AGTGCAATGA ACTTGGATTA 
TCAAAGAAAG AGTTGAAGAA GCTTCTACCT ATTGTGGACG TTAGCTCTTC TGACTCAGGT 
GCTTTTGATG GTGTTCTTGA GCTTCTTGTC CGAGCTGGTA GAAGTCTTCC TGAAGCTGTT 
ATGATGATGA TCCCTGAAGC ATGGCAAAAT GACAAAAACA TTGATCCAAG CAGGAAAGAG 
TTTTATGAAT ACTTATCTGC TCTAATGGAG CCATGGGATG GACCTGCCCT CATATCATTC 
ACTGATGGTC GCTACTTGGG TGCCACATTG GACCGTAATG GACTTCGTCC TGGTAGGTTC 
TACATAACCC ACAGTGGAAG GGTTATCATG GCTAGTGAAG TTGGGGTAGT GGATGTACCA 
CCTGAAGATG TGATGAGGAA AGGAAGACTT AACCCGGGTA TGATGCTTCT TGTCGATTTT 
GAGAAGCATA TTGTTGTTGA TGACGATGCC TTGAAGCAGC AGTATTCACT GGCAAGGCCT 
TATGGTGAGT GGCTTAAAAG GCAAAAGATT GAACTTAAAG ACATCATTGA GTCAGTTCCG 
GAGGCTGAAC GGATTGCTCC TTCAATTTCT GGGGTTGTAC CGGCCTCTAA TGATGATGAC 
TCAATGGAGA GCATGGGCAT TCATGGTTTA TTGTCTCCAC TAAAAGCCTT TGGCTACACA 
GTTGAGGCCT TGGAGATGTT GCTGCTTCCG ATGGCTAAAG ATGGCTCTGA AGCACTTGGT 
TCTATGGGAA ATGATACCCC TCTAGCTGTA ATGTCAAACA GAGAAAAATT ATGCTTTGAG 
TACTTCAAGC AAATGTTTGC TCAAGTAACA AATCCTCCAA TTGATCCTAT TCGTGAGAAA 
ATTGTTACAT CAATGGAGTG CATGATCGGT CCAGAAGGCG ATCTAACCGA AACAACTGAA 
GAGCAATGTC ACCGTCTCTC CCTAAAAGGT CCTCTTTTGA AAATCGAAGA GATGGAAGCC 
ATCAAGAAAA TGAACTACAG AGGCTGGCGG ACTAAAGTTC TGGATATAAC TTATGCAAAA 
GAAAGAGGTA CAAAAGGGTT AGAGGAGACT TTGGACCGAA TTTGTGATGA AGCAAATGAA 
GCAATAAAGG AGGGCTACAC ATTACTGGTT TTGTCTGATC GAGCTTTCTC TGCAACACGT 
GTTGCTGTGA GCTCCCTCAT GGCTGTTGGT GCCGTTCATC ACCACCTGGT TAAGACACTT 
GCGCGCACCC AAGTTGGTTT GGTAGTGGAA TCTGCTGAAC CACGTGAAGT GCATCATTTC 
TGCACACTTG TCGGATTTGG AGCAGATGCT ATCTGCCCAT ATTTGGCTGT AGAGGCCGTT 
TATAGATTAC AGGTTGATGG CAAAATACCA CCCAAATCTA ATGGGGAATT CCATTCCAAA 
GAAGAGTTGG TCAAGAAGTA TTACAAGGCA AGTAACTATG GAATGATGAA GGTTCTTGCC 
AAAATGGGAA TTTCTACTCT AGCCTCATAC AAAGGTGCTC AGATATTTGA AGCTCTTGGG 
CTTTCATCTG AAGTAATTCA GAAATGTTTT GCCGGTACCC CAAGCCGAGT GGAAGGAGCA 
ACATTTGAGA TGCTTGCACG AGATGGACTT CAATTGCATG AGTTGGCCTT TCCAACCCGT 
GGCTATGCCC CAGGAAGTGC AGAAGCTTCA GCACTTACCA ACCCTGGAAA CTATCACTGG 
AGGAAAAATG GAGAGATTCA TCTCAACGAT CCTCTTGCAA TAGCCAAATT ACAAGAGGCA 
GCACGGACCA ACAGTGTGGC CGCCTACAAG GAATACTCAA AGCGTATCAA TGAATTGAAC 
AAGCAAAGTA ATTTGCGTGG GCTCATGAAG TTTAAAGACG CAGATGTGAA AATTCCACTA 
GATGAAGTCG AGCCTGCCAG TGAAATTGTT AAGCGGTTCT GCACTGGTGC CATGAGTTAT 
GGATCAATTT CTCTGGAGGC GCATACAACC CTTGCAATGG CCATGAACAA GCTTGGAGGA 
AAGTCGAATA CAGGTGAGGG AGGGGAACTG CCATCCCGTA TGGAGCCTCT TGCGGATGGT 
TCAAGGAACC CCAAAAGAAG TTCGATCAAA CAAATTGCTA GTGGAAGATT TGGAGTTTCT 
AGCTACTATC TTACAAATGC TGATGAGCTG CAGATAAAGA TGGCTCAGGG TGCCAAACCT 
GGGGAAGGTG GGGAGCTACC AGGCCACAAG GTCATAGGAG ACATTGCTAT AACCAGAAAT 
TCCACTGCTG GTGTAGGACT TATCAGTCCA CCTCCTCATC ATGATATCTA CTCTATCGAG 
GATCTTGCCC AGCTAATTCA CGATCTTAAG AATGCCAATC CTGGCGCACG TATTAGTGTT 
AAGCTTGTAT CTGAAGCTGG TGTTGGAGTT ATTGCTAGCG GTGTGGTGAA GGGGCATGCT 
GACCATGTTC TGATAGCAGG TCATGATGGA GGTACTGGTG CATCTCGCTG GACCGGTATT 
AAAAATGCTG GTCTTCCATG GGAACTGGGT CTAGCTGAGA CACACCAAAC TCTTGTTGCC 
AATGACCTTC GTGGCCGTAC TGTTCTTCAG ACAGATGGCC AGCTAAAAAC TGGCAGAGAC 
GTTGCAGTTG CTGCACTTCT TGGTGCAGAA GAATTTGGTT TTAGTACTGC ACCCCTTATC 
ACACTAGGGT GTATCATGAT GAGAAAGTGC CACAAGAACA CCTGCCCTGT GGGAATTGCT 
ACCCAAGATC CAGTTCTCCG TGAAAAATTT GCTGGGGAGC CTGAACATGT TATTAACTTC 
TTCTTTATGC TTGCTGAGGA AGTTAGGGAG ATCATGTCTG GACTTGGTTT TAGAACTGTA 
ACTGAGATGA TTGGTCGTGC GGATATGCTT GAACTTGATA GGGAAGTTGT CAAGAACAAC 
GATAAGCTGG AGAACATTGA CCTATCTCTT TTGCTCAGAC CTGCTGCTGA GATTCGTCCT 
GGGGCTGCTC AGTACTGTGT TCAGAAACAA GATCATGGTT TAGACATGGC TTTGGATCAA 
GAGCTGATTG CATTGTCTAA ATCTGCTCTG GAGAAATCTC TTCCGGTGTA CATTGAAACT 
CCTATCTGCA ACGTTAACCG TGCTGTTGGG ACAATGCTGA GCCATGAGGT AACCAAACGT 
TACCACTTGA CTGGGCTTCC AAAGGACACA ATTCATATCA AGTTCACAGG AAGTGCTGGG 
CAGAGTCTTG GAGCTTTCTT GTGTCCTGGA ATTATGCTAG AACTTGAAGG AGATAGCAAT 
GACTATGTTG GAAAAGGGCT CTCTGGAGGT AAAGTTGTTG TTTACCCTCC AAAAGGAAGC 
AGCTTTGATC CAAAAGAAAA TATCGTTATT GGTAATGTAG CACTTTACGG TGCCACTAGT 
GGTGAAGCAT ACTTCAATGG GATGGCTGCT GAAAGGTTCT CTGTTCGTAA TTCTGGTGCT 
AAAGCTGTTG TGGAAGGTCT TGGTGATCAT GGTTGTGAGT ACATGACTGG TGGTACTGTT 
GTGGTGCTTG GAAAGACTGG TAGAAACTTT GCTGCTGGTA TGAGTGGTGG TATTGCTTAT 
GTCCTTGATG TGGACGGAAA GTTTAACACC AGATGCAACC TTGAACTGGT TGATTTGGAT 
AAAGTTGAAG ATGAGGAGGA CAAAATGACG CTCAAAATGA TGATCCAGCA ACATCAAAGA 
CACACCAACA GCCAACTTGC TCAAGAAGTT CTTGCAGACT TTGAGAATTT GCTGCCCAAG 
TTTATCAAGG TTTTCCCAAG AGATTACAAA CGTGTTTTAT CAGCCATGAA ACACGAAGAG 
GTCTCCAAGC AAGCAATCGA GCGGGCTTCT GAGGAAGCTG ACGAGACTGA AGAGAAAGAA 
CTCGAGGAGA AAGATGCATT TGCAGAACTG AAGAACATGG CAGCTGCTTC GTCAAAAGAG 
GAGATGTCAG GAAACGGAGT GGCAGCTGAA GCTAGACCTT CTAAGGTAGA TAATGCTGTT 
AAAAACGGTG GTTTCATTGC TTATGAGCGT GAGGGAGTTA AGTACAGGGA TCCCAATGTT 
CGTCTTAATG ACTGGAACGA AGTCATGGAG GAATCAAAAC CTGGACCACT CCTTACAACT 
CAGTCAGCTC GTTGCATGGA TTGTGGAACT CCATTCTGCC ACCAGGAGAA CTCTGGGTGT 
CCTCTCGGTA ATAAGATCCC TGAATTCAAT GAACTTGTCT ACCAGAACAG ATGGCAAGAA 
GCCTTGAATC GTCTACTTGA GACAAACAAC TTTCCAGAAT TTACTGGGCG AGTATGCCCT 
GCACCATGTG AAGGTTCTTG TGTTCTTGGA ATAATTGAGA ACCCTGTTTC TATCAAAAGC 
ATTGAATGTG CTATTATCGA TAAAGCCTTT GAGGAAGGGT GGATGGTACC AAGGCCTCCT 
CTCAAGAGAA CAGGGAAAAA GGTTGCTATT ATCGGAAGTG GACCGGCTGG GTTAGCTGCG 
GCTGACCAGC TGAACAAAAT GGGCCATTTA GTAACCGTGT ATGAGCGCTC GGACAGAATT 
GGTGGGCTGA TGATGTATGG AGTACCAAAC ATGAAGACTG ACAAAATTGA CGTCGTTCAA 
CGGCGGGTTG ATCTTATGAC CAAAGAAGGT ATCAACTTTG TGGTCAATGC CAATATTGGA 
AAGGACCCAT CTTACTCCCT TGATGGACTT AAGGAAGAGA ATGATGCAAT CGTTCTTGCT 
GTTGGTTCCA CAAAACCAAG AGATCTTCCA GTGCCTGGTC GTGATCTATC TGGTGTTCAC 
TTTGCCATGG AGTTTCTTCA TGCAAACACC AAAAGTTTAC TTGACAGCAA TCATGAGGAT 
GGCAATTACA TTTCTGCAAA AGGGAAAAAG GTTGTTGTTA TTGGTGGAGG TGATACCGGC 
ACAGATTGCA TCGGAACATC TATCCGCCAT GGATGTACCA ACATTGTAAA CCTAGAACTT 
CTTCCTCAGC CACCCTCAAC AAGAGCTCCT GGAAACCCTT GGCCACAGTG GCCTCGTGTA 
TTCCGTATTG ACTACGGACA TCAAGAAGCT ACCACCAAAT TCGGAAAAGA CCCTAGGACC 
TATGAGGTCT TAACGAAGAG GTTCATAGGA GATGACAATG GAAACGTGAA GGGGCTTGAA 
CTTGTGCGTG TGAGTTGGGA GAAGGATGAA ACCGGGAGGT TCCAGTTCAA GGAGATCGAA 
GGCTCTGAGG AAATCATTGA AGCAGACCTC GTTTTCTTGG CCATGGGTTT CCTTGGACCT 
GAGCCAACAC TTGCTGAGAA GCTAGGTCTC GAATGTGACA ACAGGTCAAA CTTTAAGGCC 
GAGTATGGCC GGTTTTCCAC AACCGTGGAA GGTGTTTTCG CAGCAGGTGA TTGTCGGAGA 
GGCCAGTCTT TGGTTGTCTG GGCTATCTCA GAAGGACGTC AAGCTGCAGA TCAGGTAGAC 
AAATTCCTCA CCAAAACGGA TGATGACGAA GACGCCAAGC TACAACAAGA CCTTAACCAG 
ATGAAACACA ACACTATCAC AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLT1 ( NM_124725.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_200158.2 Arabidopsis thaliana NADH-dependent glutamate synthase 1 (GLT1), mRNA.

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CloneID	OAb21327
Clone ID Related Accession (Same CDS sequence)	NM_001203600.1 , NM_001203601.1 , NM_124725.5
Accession Version	NM_124725.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NADH-dependent glutamate synthase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_124725.4.

ATGTCGGCAG CTTCTTCTAG CTCCGTTCTT CACCTCCGAA CCAACCAACA ACTGTTATCA 
CTCCGATCTC TCAAAAATTC CACCTCCGTT GCTTCTCAAT TGGCCGTTAC GTCTGGTGTT 
AGCCGTAGAA GATCGTGTAC GGCGCGATGC TCTGTGAAGA AACCGGTGAT TCCTGAGAGT 
CCTTTCCTAG GGACTCGTGT GCGGAGATCC GGATCCGAGA CGCTTCAGTT CTGGAGATCA 
GATGGTCCTG GACGGTCTGC GAAGCTTCGA ACGGTGGTTA AATCGTCATT TTCCGCTGTT 
CCGGAGAAGC CTCTTGGTCT TTATGATCCT TCCTATGATA AAGACTCTTG TGGTGTTGGT 
TTTGTAGCAG AGTTGTCCGG CGAGACCACC CGCAAAACGG TGACTGATTC GTTGGAGATG 
TTGATACGGA TGACTCATAG AGGTGCTTGT GGTTGTGAGA GCAACACTGG TGACGGCGCT 
GGGATTCTTG TTGGTCTGCC TCATGATTTC TATGCAGAGG CTGCGACAGA GCTTGGCTTT 
GTACTTCCTT CTGCTGGAAA TTATGCTGTT GGTATGTTCT TCTTGCCAAC TGTTGAAAGC 
AGGAGAGAAG AAAGCAAGAA TGTTTTCACT AAGGTTGCTG AATCACTTGG CCACTCGGTT 
CTTGGATGGA GATTAGTGCC TACCGATAAT TCTGGACTGG GAAACTCCGC TTTGCAGACT 
GAACCGATTA TTGCGCAAGT TTTCCTTACT CCTACAACTA AATCTAAAGC TGACTTTGAG 
CAACAGATGT ATATCCTTAG AAGGGTTTCT ATGGTTGCTA TTCGAGCTGC GTTGAACCTT 
CAACATGGTG CGATGAAGGA CTTCTACATC TGTTCGTTGT CTTCTAGGAC TATCGTCTAC 
AAAGGACAAT TGAAGCCTGA TCAACTAAAG GACTATTACT ATGCGGATCT TGGGAGTGAA 
AGGTTTACGA GCTACATGGC CCTGGTTCAT TCTCGGTTCT CTACCAATAC ATTTCCCAGC 
TGGGATCGTG CTCAGCCGAT GCGTGTTTTG GGCCACAATG GGGAGATCAA TACACTCAGG 
GGAAACGTGA ACTGGATGAG GGCACGTGAG GGTCTGCTCA AGTGCAATGA ACTTGGATTA 
TCAAAGAAAG AGTTGAAGAA GCTTCTACCT ATTGTGGACG TTAGCTCTTC TGACTCAGGT 
GCTTTTGATG GTGTTCTTGA GCTTCTTGTC CGAGCTGGTA GAAGTCTTCC TGAAGCTGTT 
ATGATGATGA TCCCTGAAGC ATGGCAAAAT GACAAAAACA TTGATCCAAG CAGGAAAGAG 
TTTTATGAAT ACTTATCTGC TCTAATGGAG CCATGGGATG GACCTGCCCT CATATCATTC 
ACTGATGGTC GCTACTTGGG TGCCACATTG GACCGTAATG GACTTCGTCC TGGTAGGTTC 
TACATAACCC ACAGTGGAAG GGTTATCATG GCTAGTGAAG TTGGGGTAGT GGATGTACCA 
CCTGAAGATG TGATGAGGAA AGGAAGACTT AACCCGGGTA TGATGCTTCT TGTCGATTTT 
GAGAAGCATA TTGTTGTTGA TGACGATGCC TTGAAGCAGC AGTATTCACT GGCAAGGCCT 
TATGGTGAGT GGCTTAAAAG GCAAAAGATT GAACTTAAAG ACATCATTGA GTCAGTTCCG 
GAGGCTGAAC GGATTGCTCC TTCAATTTCT GGGGTTGTAC CGGCCTCTAA TGATGATGAC 
TCAATGGAGA GCATGGGCAT TCATGGTTTA TTGTCTCCAC TAAAAGCCTT TGGCTACACA 
GTTGAGGCCT TGGAGATGTT GCTGCTTCCG ATGGCTAAAG ATGGCTCTGA AGCACTTGGT 
TCTATGGGAA ATGATACCCC TCTAGCTGTA ATGTCAAACA GAGAAAAATT ATGCTTTGAG 
TACTTCAAGC AAATGTTTGC TCAAGTAACA AATCCTCCAA TTGATCCTAT TCGTGAGAAA 
ATTGTTACAT CAATGGAGTG CATGATCGGT CCAGAAGGCG ATCTAACCGA AACAACTGAA 
GAGCAATGTC ACCGTCTCTC CCTAAAAGGT CCTCTTTTGA AAATCGAAGA GATGGAAGCC 
ATCAAGAAAA TGAACTACAG AGGCTGGCGG ACTAAAGTTC TGGATATAAC TTATGCAAAA 
GAAAGAGGTA CAAAAGGGTT AGAGGAGACT TTGGACCGAA TTTGTGATGA AGCAAATGAA 
GCAATAAAGG AGGGCTACAC ATTACTGGTT TTGTCTGATC GAGCTTTCTC TGCAACACGT 
GTTGCTGTGA GCTCCCTCAT GGCTGTTGGT GCCGTTCATC ACCACCTGGT TAAGACACTT 
GCGCGCACCC AAGTTGGTTT GGTAGTGGAA TCTGCTGAAC CACGTGAAGT GCATCATTTC 
TGCACACTTG TCGGATTTGG AGCAGATGCT ATCTGCCCAT ATTTGGCTGT AGAGGCCGTT 
TATAGATTAC AGGTTGATGG CAAAATACCA CCCAAATCTA ATGGGGAATT CCATTCCAAA 
GAAGAGTTGG TCAAGAAGTA TTACAAGGCA AGTAACTATG GAATGATGAA GGTTCTTGCC 
AAAATGGGAA TTTCTACTCT AGCCTCATAC AAAGGTGCTC AGATATTTGA AGCTCTTGGG 
CTTTCATCTG AAGTAATTCA GAAATGTTTT GCCGGTACCC CAAGCCGAGT GGAAGGAGCA 
ACATTTGAGA TGCTTGCACG AGATGGACTT CAATTGCATG AGTTGGCCTT TCCAACCCGT 
GGCTATGCCC CAGGAAGTGC AGAAGCTTCA GCACTTACCA ACCCTGGAAA CTATCACTGG 
AGGAAAAATG GAGAGATTCA TCTCAACGAT CCTCTTGCAA TAGCCAAATT ACAAGAGGCA 
GCACGGACCA ACAGTGTGGC CGCCTACAAG GAATACTCAA AGCGTATCAA TGAATTGAAC 
AAGCAAAGTA ATTTGCGTGG GCTCATGAAG TTTAAAGACG CAGATGTGAA AATTCCACTA 
GATGAAGTCG AGCCTGCCAG TGAAATTGTT AAGCGGTTCT GCACTGGTGC CATGAGTTAT 
GGATCAATTT CTCTGGAGGC GCATACAACC CTTGCAATGG CCATGAACAA GCTTGGAGGA 
AAGTCGAATA CAGGTGAGGG AGGGGAACTG CCATCCCGTA TGGAGCCTCT TGCGGATGGT 
TCAAGGAACC CCAAAAGAAG TTCGATCAAA CAAATTGCTA GTGGAAGATT TGGAGTTTCT 
AGCTACTATC TTACAAATGC TGATGAGCTG CAGATAAAGA TGGCTCAGGG TGCCAAACCT 
GGGGAAGGTG GGGAGCTACC AGGCCACAAG GTCATAGGAG ACATTGCTAT AACCAGAAAT 
TCCACTGCTG GTGTAGGACT TATCAGTCCA CCTCCTCATC ATGATATCTA CTCTATCGAG 
GATCTTGCCC AGCTAATTCA CGATCTTAAG AATGCCAATC CTGGCGCACG TATTAGTGTT 
AAGCTTGTAT CTGAAGCTGG TGTTGGAGTT ATTGCTAGCG GTGTGGTGAA GGGGCATGCT 
GACCATGTTC TGATAGCAGG TCATGATGGA GGTACTGGTG CATCTCGCTG GACCGGTATT 
AAAAATGCTG GTCTTCCATG GGAACTGGGT CTAGCTGAGA CACACCAAAC TCTTGTTGCC 
AATGACCTTC GTGGCCGTAC TGTTCTTCAG ACAGATGGCC AGCTAAAAAC TGGCAGAGAC 
GTTGCAGTTG CTGCACTTCT TGGTGCAGAA GAATTTGGTT TTAGTACTGC ACCCCTTATC 
ACACTAGGGT GTATCATGAT GAGAAAGTGC CACAAGAACA CCTGCCCTGT GGGAATTGCT 
ACCCAAGATC CAGTTCTCCG TGAAAAATTT GCTGGGGAGC CTGAACATGT TATTAACTTC 
TTCTTTATGC TTGCTGAGGA AGTTAGGGAG ATCATGTCTG GACTTGGTTT TAGAACTGTA 
ACTGAGATGA TTGGTCGTGC GGATATGCTT GAACTTGATA GGGAAGTTGT CAAGAACAAC 
GATAAGCTGG AGAACATTGA CCTATCTCTT TTGCTCAGAC CTGCTGCTGA GATTCGTCCT 
GGGGCTGCTC AGTACTGTGT TCAGAAACAA GATCATGGTT TAGACATGGC TTTGGATCAA 
GAGCTGATTG CATTGTCTAA ATCTGCTCTG GAGAAATCTC TTCCGGTGTA CATTGAAACT 
CCTATCTGCA ACGTTAACCG TGCTGTTGGG ACAATGCTGA GCCATGAGGT AACCAAACGT 
TACCACTTGA CTGGGCTTCC AAAGGACACA ATTCATATCA AGTTCACAGG AAGTGCTGGG 
CAGAGTCTTG GAGCTTTCTT GTGTCCTGGA ATTATGCTAG AACTTGAAGG AGATAGCAAT 
GACTATGTTG GAAAAGGGCT CTCTGGAGGT AAAGTTGTTG TTTACCCTCC AAAAGGAAGC 
AGCTTTGATC CAAAAGAAAA TATCGTTATT GGTAATGTAG CACTTTACGG TGCCACTAGT 
GGTGAAGCAT ACTTCAATGG GATGGCTGCT GAAAGGTTCT CTGTTCGTAA TTCTGGTGCT 
AAAGCTGTTG TGGAAGGTCT TGGTGATCAT GGTTGTGAGT ACATGACTGG TGGTACTGTT 
GTGGTGCTTG GAAAGACTGG TAGAAACTTT GCTGCTGGTA TGAGTGGTGG TATTGCTTAT 
GTCCTTGATG TGGACGGAAA GTTTAACACC AGATGCAACC TTGAACTGGT TGATTTGGAT 
AAAGTTGAAG ATGAGGAGGA CAAAATGACG CTCAAAATGA TGATCCAGCA ACATCAAAGA 
CACACCAACA GCCAACTTGC TCAAGAAGTT CTTGCAGACT TTGAGAATTT GCTGCCCAAG 
TTTATCAAGG TTTTCCCAAG AGATTACAAA CGTGTTTTAT CAGCCATGAA ACACGAAGAG 
GTCTCCAAGC AAGCAATCGA GCGGGCTTCT GAGGAAGCTG ACGAGACTGA AGAGAAAGAA 
CTCGAGGAGA AAGATGCATT TGCAGAACTG AAGAACATGG CAGCTGCTTC GTCAAAAGAG 
GAGATGTCAG GAAACGGAGT GGCAGCTGAA GCTAGACCTT CTAAGGTAGA TAATGCTGTT 
AAAAACGGTG GTTTCATTGC TTATGAGCGT GAGGGAGTTA AGTACAGGGA TCCCAATGTT 
CGTCTTAATG ACTGGAACGA AGTCATGGAG GAATCAAAAC CTGGACCACT CCTTACAACT 
CAGTCAGCTC GTTGCATGGA TTGTGGAACT CCATTCTGCC ACCAGGAGAA CTCTGGGTGT 
CCTCTCGGTA ATAAGATCCC TGAATTCAAT GAACTTGTCT ACCAGAACAG ATGGCAAGAA 
GCCTTGAATC GTCTACTTGA GACAAACAAC TTTCCAGAAT TTACTGGGCG AGTATGCCCT 
GCACCATGTG AAGGTTCTTG TGTTCTTGGA ATAATTGAGA ACCCTGTTTC TATCAAAAGC 
ATTGAATGTG CTATTATCGA TAAAGCCTTT GAGGAAGGGT GGATGGTACC AAGGCCTCCT 
CTCAAGAGAA CAGGGAAAAA GGTTGCTATT ATCGGAAGTG GACCGGCTGG GTTAGCTGCG 
GCTGACCAGC TGAACAAAAT GGGCCATTTA GTAACCGTGT ATGAGCGCTC GGACAGAATT 
GGTGGGCTGA TGATGTATGG AGTACCAAAC ATGAAGACTG ACAAAATTGA CGTCGTTCAA 
CGGCGGGTTG ATCTTATGAC CAAAGAAGGT ATCAACTTTG TGGTCAATGC CAATATTGGA 
AAGGACCCAT CTTACTCCCT TGATGGACTT AAGGAAGAGA ATGATGCAAT CGTTCTTGCT 
GTTGGTTCCA CAAAACCAAG AGATCTTCCA GTGCCTGGTC GTGATCTATC TGGTGTTCAC 
TTTGCCATGG AGTTTCTTCA TGCAAACACC AAAAGTTTAC TTGACAGCAA TCATGAGGAT 
GGCAATTACA TTTCTGCAAA AGGGAAAAAG GTTGTTGTTA TTGGTGGAGG TGATACCGGC 
ACAGATTGCA TCGGAACATC TATCCGCCAT GGATGTACCA ACATTGTAAA CCTAGAACTT 
CTTCCTCAGC CACCCTCAAC AAGAGCTCCT GGAAACCCTT GGCCACAGTG GCCTCGTGTA 
TTCCGTATTG ACTACGGACA TCAAGAAGCT ACCACCAAAT TCGGAAAAGA CCCTAGGACC 
TATGAGGTCT TAACGAAGAG GTTCATAGGA GATGACAATG GAAACGTGAA GGGGCTTGAA 
CTTGTGCGTG TGAGTTGGGA GAAGGATGAA ACCGGGAGGT TCCAGTTCAA GGAGATCGAA 
GGCTCTGAGG AAATCATTGA AGCAGACCTC GTTTTCTTGG CCATGGGTTT CCTTGGACCT 
GAGCCAACAC TTGCTGAGAA GCTAGGTCTC GAATGTGACA ACAGGTCAAA CTTTAAGGCC 
GAGTATGGCC GGTTTTCCAC AACCGTGGAA GGTGTTTTCG CAGCAGGTGA TTGTCGGAGA 
GGCCAGTCTT TGGTTGTCTG GGCTATCTCA GAAGGACGTC AAGCTGCAGA TCAGGTAGAC 
AAATTCCTCA CCAAAACGGA TGATGACGAA GACGCCAAGC TACAACAAGA CCTTAACCAG 
ATGAAACACA ACACTATCAC AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_200158.2
CDS	332..6958
Translation	MSAASSSSVLHLRTNQQLLSLRSLKNSTSVASQLAVTSGVSRRRSCTARCSVKKPVIPESPFLGTRVRRSGSETLQFWRSDGPGRSAKLRTVVKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTRKTVTDSLEMLIRMTHRGACGCESNTGDGAGILVGLPHDFYAEAATELGFVLPSAGNYAVGMFFLPTVESRREESKNVFTKVAESLGHSVLGWRLVPTDNSGLGNSALQTEPIIAQVFLTPTTKSKADFEQQMYILRRVSMVAIRAALNLQHGAMKDFYICSLSSRTIVYKGQLKPDQLKDYYYADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLKCNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNIDPSRKEFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEVGVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSLARPYGEWLKRQKIELKDIIESVPEAERIAPSISGVVPASNDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKDGSEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLKIEEMEAIKKMNYRGWRTKVLDITYAKERGTKGLEETLDRICDEANEAIKEGYTLLVLSDRAFSATRVAVSSLMAVGAVHHHLVKTLARTQVGLVVESAEPREVHHFCTLVGFGADAICPYLAVEAVYRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVIQKCFAGTPSRVEGATFEMLARDGLQLHELAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSKRINELNKQSNLRGLMKFKDADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKLGGKSNTGEGGELPSRMEPLADGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTVTEMIGRADMLELDREVVKNNDKLENIDLSLLLRPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSLPVYIETPICNVNRAVGTMLSHEVTKRYHLTGLPKDTIHIKFTGSAGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGAKAVVEGLGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFNTRCNLELVDLDKVEDEEDKMTLKMMIQQHQRHTNSQLAQEVLADFENLLPKFIKVFPRDYKRVLSAMKHEEVSKQAIERASEEADETEEKELEEKDAFAELKNMAAASSKEEMSGNGVAAEARPSKVDNAVKNGGFIAYEREGVKYRDPNVRLNDWNEVMEESKPGPLLTTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFEEGWMVPRPPLKRTGKKVAIIGSGPAGLAAADQLNKMGHLVTVYERSDRIGGLMMYGVPNMKTDKIDVVQRRVDLMTKEGINFVVNANIGKDPSYSLDGLKEENDAIVLAVGSTKPRDLPVPGRDLSGVHFAMEFLHANTKSLLDSNHEDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQEATTKFGKDPRTYEVLTKRFIGDDNGNVKGLELVRVSWEKDETGRFQFKEIEGSEEIIEADLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWAISEGRQAADQVDKFLTKTDDDEDAKLQQDLNQMKHNTITN

Target ORF information:

RefSeq Version	NM_124725.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NADH-dependent glutamate synthase 1 (GLT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_124725.5

ORF Insert Sequence:

ATGTCGGCAG CTTCTTCTAG CTCCGTTCTT CACCTCCGAA CCAACCAACA ACTGTTATCA 
CTCCGATCTC TCAAAAATTC CACCTCCGTT GCTTCTCAAT TGGCCGTTAC GTCTGGTGTT 
AGCCGTAGAA GATCGTGTAC GGCGCGATGC TCTGTGAAGA AACCGGTGAT TCCTGAGAGT 
CCTTTCCTAG GGACTCGTGT GCGGAGATCC GGATCCGAGA CGCTTCAGTT CTGGAGATCA 
GATGGTCCTG GACGGTCTGC GAAGCTTCGA ACGGTGGTTA AATCGTCATT TTCCGCTGTT 
CCGGAGAAGC CTCTTGGTCT TTATGATCCT TCCTATGATA AAGACTCTTG TGGTGTTGGT 
TTTGTAGCAG AGTTGTCCGG CGAGACCACC CGCAAAACGG TGACTGATTC GTTGGAGATG 
TTGATACGGA TGACTCATAG AGGTGCTTGT GGTTGTGAGA GCAACACTGG TGACGGCGCT 
GGGATTCTTG TTGGTCTGCC TCATGATTTC TATGCAGAGG CTGCGACAGA GCTTGGCTTT 
GTACTTCCTT CTGCTGGAAA TTATGCTGTT GGTATGTTCT TCTTGCCAAC TGTTGAAAGC 
AGGAGAGAAG AAAGCAAGAA TGTTTTCACT AAGGTTGCTG AATCACTTGG CCACTCGGTT 
CTTGGATGGA GATTAGTGCC TACCGATAAT TCTGGACTGG GAAACTCCGC TTTGCAGACT 
GAACCGATTA TTGCGCAAGT TTTCCTTACT CCTACAACTA AATCTAAAGC TGACTTTGAG 
CAACAGATGT ATATCCTTAG AAGGGTTTCT ATGGTTGCTA TTCGAGCTGC GTTGAACCTT 
CAACATGGTG CGATGAAGGA CTTCTACATC TGTTCGTTGT CTTCTAGGAC TATCGTCTAC 
AAAGGACAAT TGAAGCCTGA TCAACTAAAG GACTATTACT ATGCGGATCT TGGGAGTGAA 
AGGTTTACGA GCTACATGGC CCTGGTTCAT TCTCGGTTCT CTACCAATAC ATTTCCCAGC 
TGGGATCGTG CTCAGCCGAT GCGTGTTTTG GGCCACAATG GGGAGATCAA TACACTCAGG 
GGAAACGTGA ACTGGATGAG GGCACGTGAG GGTCTGCTCA AGTGCAATGA ACTTGGATTA 
TCAAAGAAAG AGTTGAAGAA GCTTCTACCT ATTGTGGACG TTAGCTCTTC TGACTCAGGT 
GCTTTTGATG GTGTTCTTGA GCTTCTTGTC CGAGCTGGTA GAAGTCTTCC TGAAGCTGTT 
ATGATGATGA TCCCTGAAGC ATGGCAAAAT GACAAAAACA TTGATCCAAG CAGGAAAGAG 
TTTTATGAAT ACTTATCTGC TCTAATGGAG CCATGGGATG GACCTGCCCT CATATCATTC 
ACTGATGGTC GCTACTTGGG TGCCACATTG GACCGTAATG GACTTCGTCC TGGTAGGTTC 
TACATAACCC ACAGTGGAAG GGTTATCATG GCTAGTGAAG TTGGGGTAGT GGATGTACCA 
CCTGAAGATG TGATGAGGAA AGGAAGACTT AACCCGGGTA TGATGCTTCT TGTCGATTTT 
GAGAAGCATA TTGTTGTTGA TGACGATGCC TTGAAGCAGC AGTATTCACT GGCAAGGCCT 
TATGGTGAGT GGCTTAAAAG GCAAAAGATT GAACTTAAAG ACATCATTGA GTCAGTTCCG 
GAGGCTGAAC GGATTGCTCC TTCAATTTCT GGGGTTGTAC CGGCCTCTAA TGATGATGAC 
TCAATGGAGA GCATGGGCAT TCATGGTTTA TTGTCTCCAC TAAAAGCCTT TGGCTACACA 
GTTGAGGCCT TGGAGATGTT GCTGCTTCCG ATGGCTAAAG ATGGCTCTGA AGCACTTGGT 
TCTATGGGAA ATGATACCCC TCTAGCTGTA ATGTCAAACA GAGAAAAATT ATGCTTTGAG 
TACTTCAAGC AAATGTTTGC TCAAGTAACA AATCCTCCAA TTGATCCTAT TCGTGAGAAA 
ATTGTTACAT CAATGGAGTG CATGATCGGT CCAGAAGGCG ATCTAACCGA AACAACTGAA 
GAGCAATGTC ACCGTCTCTC CCTAAAAGGT CCTCTTTTGA AAATCGAAGA GATGGAAGCC 
ATCAAGAAAA TGAACTACAG AGGCTGGCGG ACTAAAGTTC TGGATATAAC TTATGCAAAA 
GAAAGAGGTA CAAAAGGGTT AGAGGAGACT TTGGACCGAA TTTGTGATGA AGCAAATGAA 
GCAATAAAGG AGGGCTACAC ATTACTGGTT TTGTCTGATC GAGCTTTCTC TGCAACACGT 
GTTGCTGTGA GCTCCCTCAT GGCTGTTGGT GCCGTTCATC ACCACCTGGT TAAGACACTT 
GCGCGCACCC AAGTTGGTTT GGTAGTGGAA TCTGCTGAAC CACGTGAAGT GCATCATTTC 
TGCACACTTG TCGGATTTGG AGCAGATGCT ATCTGCCCAT ATTTGGCTGT AGAGGCCGTT 
TATAGATTAC AGGTTGATGG CAAAATACCA CCCAAATCTA ATGGGGAATT CCATTCCAAA 
GAAGAGTTGG TCAAGAAGTA TTACAAGGCA AGTAACTATG GAATGATGAA GGTTCTTGCC 
AAAATGGGAA TTTCTACTCT AGCCTCATAC AAAGGTGCTC AGATATTTGA AGCTCTTGGG 
CTTTCATCTG AAGTAATTCA GAAATGTTTT GCCGGTACCC CAAGCCGAGT GGAAGGAGCA 
ACATTTGAGA TGCTTGCACG AGATGGACTT CAATTGCATG AGTTGGCCTT TCCAACCCGT 
GGCTATGCCC CAGGAAGTGC AGAAGCTTCA GCACTTACCA ACCCTGGAAA CTATCACTGG 
AGGAAAAATG GAGAGATTCA TCTCAACGAT CCTCTTGCAA TAGCCAAATT ACAAGAGGCA 
GCACGGACCA ACAGTGTGGC CGCCTACAAG GAATACTCAA AGCGTATCAA TGAATTGAAC 
AAGCAAAGTA ATTTGCGTGG GCTCATGAAG TTTAAAGACG CAGATGTGAA AATTCCACTA 
GATGAAGTCG AGCCTGCCAG TGAAATTGTT AAGCGGTTCT GCACTGGTGC CATGAGTTAT 
GGATCAATTT CTCTGGAGGC GCATACAACC CTTGCAATGG CCATGAACAA GCTTGGAGGA 
AAGTCGAATA CAGGTGAGGG AGGGGAACTG CCATCCCGTA TGGAGCCTCT TGCGGATGGT 
TCAAGGAACC CCAAAAGAAG TTCGATCAAA CAAATTGCTA GTGGAAGATT TGGAGTTTCT 
AGCTACTATC TTACAAATGC TGATGAGCTG CAGATAAAGA TGGCTCAGGG TGCCAAACCT 
GGGGAAGGTG GGGAGCTACC AGGCCACAAG GTCATAGGAG ACATTGCTAT AACCAGAAAT 
TCCACTGCTG GTGTAGGACT TATCAGTCCA CCTCCTCATC ATGATATCTA CTCTATCGAG 
GATCTTGCCC AGCTAATTCA CGATCTTAAG AATGCCAATC CTGGCGCACG TATTAGTGTT 
AAGCTTGTAT CTGAAGCTGG TGTTGGAGTT ATTGCTAGCG GTGTGGTGAA GGGGCATGCT 
GACCATGTTC TGATAGCAGG TCATGATGGA GGTACTGGTG CATCTCGCTG GACCGGTATT 
AAAAATGCTG GTCTTCCATG GGAACTGGGT CTAGCTGAGA CACACCAAAC TCTTGTTGCC 
AATGACCTTC GTGGCCGTAC TGTTCTTCAG ACAGATGGCC AGCTAAAAAC TGGCAGAGAC 
GTTGCAGTTG CTGCACTTCT TGGTGCAGAA GAATTTGGTT TTAGTACTGC ACCCCTTATC 
ACACTAGGGT GTATCATGAT GAGAAAGTGC CACAAGAACA CCTGCCCTGT GGGAATTGCT 
ACCCAAGATC CAGTTCTCCG TGAAAAATTT GCTGGGGAGC CTGAACATGT TATTAACTTC 
TTCTTTATGC TTGCTGAGGA AGTTAGGGAG ATCATGTCTG GACTTGGTTT TAGAACTGTA 
ACTGAGATGA TTGGTCGTGC GGATATGCTT GAACTTGATA GGGAAGTTGT CAAGAACAAC 
GATAAGCTGG AGAACATTGA CCTATCTCTT TTGCTCAGAC CTGCTGCTGA GATTCGTCCT 
GGGGCTGCTC AGTACTGTGT TCAGAAACAA GATCATGGTT TAGACATGGC TTTGGATCAA 
GAGCTGATTG CATTGTCTAA ATCTGCTCTG GAGAAATCTC TTCCGGTGTA CATTGAAACT 
CCTATCTGCA ACGTTAACCG TGCTGTTGGG ACAATGCTGA GCCATGAGGT AACCAAACGT 
TACCACTTGA CTGGGCTTCC AAAGGACACA ATTCATATCA AGTTCACAGG AAGTGCTGGG 
CAGAGTCTTG GAGCTTTCTT GTGTCCTGGA ATTATGCTAG AACTTGAAGG AGATAGCAAT 
GACTATGTTG GAAAAGGGCT CTCTGGAGGT AAAGTTGTTG TTTACCCTCC AAAAGGAAGC 
AGCTTTGATC CAAAAGAAAA TATCGTTATT GGTAATGTAG CACTTTACGG TGCCACTAGT 
GGTGAAGCAT ACTTCAATGG GATGGCTGCT GAAAGGTTCT CTGTTCGTAA TTCTGGTGCT 
AAAGCTGTTG TGGAAGGTCT TGGTGATCAT GGTTGTGAGT ACATGACTGG TGGTACTGTT 
GTGGTGCTTG GAAAGACTGG TAGAAACTTT GCTGCTGGTA TGAGTGGTGG TATTGCTTAT 
GTCCTTGATG TGGACGGAAA GTTTAACACC AGATGCAACC TTGAACTGGT TGATTTGGAT 
AAAGTTGAAG ATGAGGAGGA CAAAATGACG CTCAAAATGA TGATCCAGCA ACATCAAAGA 
CACACCAACA GCCAACTTGC TCAAGAAGTT CTTGCAGACT TTGAGAATTT GCTGCCCAAG 
TTTATCAAGG TTTTCCCAAG AGATTACAAA CGTGTTTTAT CAGCCATGAA ACACGAAGAG 
GTCTCCAAGC AAGCAATCGA GCGGGCTTCT GAGGAAGCTG ACGAGACTGA AGAGAAAGAA 
CTCGAGGAGA AAGATGCATT TGCAGAACTG AAGAACATGG CAGCTGCTTC GTCAAAAGAG 
GAGATGTCAG GAAACGGAGT GGCAGCTGAA GCTAGACCTT CTAAGGTAGA TAATGCTGTT 
AAAAACGGTG GTTTCATTGC TTATGAGCGT GAGGGAGTTA AGTACAGGGA TCCCAATGTT 
CGTCTTAATG ACTGGAACGA AGTCATGGAG GAATCAAAAC CTGGACCACT CCTTACAACT 
CAGTCAGCTC GTTGCATGGA TTGTGGAACT CCATTCTGCC ACCAGGAGAA CTCTGGGTGT 
CCTCTCGGTA ATAAGATCCC TGAATTCAAT GAACTTGTCT ACCAGAACAG ATGGCAAGAA 
GCCTTGAATC GTCTACTTGA GACAAACAAC TTTCCAGAAT TTACTGGGCG AGTATGCCCT 
GCACCATGTG AAGGTTCTTG TGTTCTTGGA ATAATTGAGA ACCCTGTTTC TATCAAAAGC 
ATTGAATGTG CTATTATCGA TAAAGCCTTT GAGGAAGGGT GGATGGTACC AAGGCCTCCT 
CTCAAGAGAA CAGGGAAAAA GGTTGCTATT ATCGGAAGTG GACCGGCTGG GTTAGCTGCG 
GCTGACCAGC TGAACAAAAT GGGCCATTTA GTAACCGTGT ATGAGCGCTC GGACAGAATT 
GGTGGGCTGA TGATGTATGG AGTACCAAAC ATGAAGACTG ACAAAATTGA CGTCGTTCAA 
CGGCGGGTTG ATCTTATGAC CAAAGAAGGT ATCAACTTTG TGGTCAATGC CAATATTGGA 
AAGGACCCAT CTTACTCCCT TGATGGACTT AAGGAAGAGA ATGATGCAAT CGTTCTTGCT 
GTTGGTTCCA CAAAACCAAG AGATCTTCCA GTGCCTGGTC GTGATCTATC TGGTGTTCAC 
TTTGCCATGG AGTTTCTTCA TGCAAACACC AAAAGTTTAC TTGACAGCAA TCATGAGGAT 
GGCAATTACA TTTCTGCAAA AGGGAAAAAG GTTGTTGTTA TTGGTGGAGG TGATACCGGC 
ACAGATTGCA TCGGAACATC TATCCGCCAT GGATGTACCA ACATTGTAAA CCTAGAACTT 
CTTCCTCAGC CACCCTCAAC AAGAGCTCCT GGAAACCCTT GGCCACAGTG GCCTCGTGTA 
TTCCGTATTG ACTACGGACA TCAAGAAGCT ACCACCAAAT TCGGAAAAGA CCCTAGGACC 
TATGAGGTCT TAACGAAGAG GTTCATAGGA GATGACAATG GAAACGTGAA GGGGCTTGAA 
CTTGTGCGTG TGAGTTGGGA GAAGGATGAA ACCGGGAGGT TCCAGTTCAA GGAGATCGAA 
GGCTCTGAGG AAATCATTGA AGCAGACCTC GTTTTCTTGG CCATGGGTTT CCTTGGACCT 
GAGCCAACAC TTGCTGAGAA GCTAGGTCTC GAATGTGACA ACAGGTCAAA CTTTAAGGCC 
GAGTATGGCC GGTTTTCCAC AACCGTGGAA GGTGTTTTCG CAGCAGGTGA TTGTCGGAGA 
GGCCAGTCTT TGGTTGTCTG GGCTATCTCA GAAGGACGTC AAGCTGCAGA TCAGGTAGAC 
AAATTCCTCA CCAAAACGGA TGATGACGAA GACGCCAAGC TACAACAAGA CCTTAACCAG 
ATGAAACACA ACACTATCAC AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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GLT1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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