HMA2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	HMA2
Entrez Gene ID	829134
Full Name	heavy metal atpase 2
Synonyms	ARABIDOPSIS HEAVY METAL ATPASE 2,ATHMA2,F6G3.140,F6G3_140,heavy metal atpase 2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_119157.4	NP_194740.1	heavy metal atpase 2
NM_001341993.1	NP_001320088.1	heavy metal atpase 2

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	HMA4	NP_179501.1
Arabidopsis thaliana (thale cress)	HMA3	NP_194741.2
Oryza sativa (rice)	Os07g0232900	NP_001059240.2
Arabidopsis thaliana (thale cress)	HMA2	NP_194740.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The molecular structures of zinc-binding- HMA4 and -HMA7 proteins have been determined and compared in order to provide insights into the structural basis for the differences in their metal binding properties.
Title: Metal binding affinities of Arabidopsis zinc and copper transporters: selectivities match the relative, but not the absolute, affinities of their amino-terminal domains.

The near-complete abolition of root-to-shoot Cd translocation resulting from the loss of function of HMA2 and HMA4 demonstrates they are the major mechanism for Cd translocation in Arabidopsis thaliana.
Title: HMA P-type ATPases are the major mechanism for root-to-shoot Cd translocation in Arabidopsis thaliana.

observations suggest that the N-terminal domain of HMA2 is essential for function in planta while the C-terminal domain, although not essential for function, may contain a signal important for the subcellular localization of the protein.
Title: Functional analysis of the heavy metal binding domains of the Zn/Cd-transporting ATPase, HMA2, in Arabidopsis thaliana.

plant Zn2+-ATPase N-terminal metal binding domains (N-MBDs) have folding & function similar to Cu+-ATPase N-MBDs. unique Zn2+ coordination via two thiols and a carboxyl group provides selective binding of the activating metals to these regulatory domains
Title: Novel Zn2+ coordination by the regulatory N-terminus metal binding domain of Arabidopsis thaliana Zn(2+)-ATPase HMA2.

The following HMA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16724	NM_119157.4 Latest version!	Arabidopsis thaliana heavy metal atpase 2 (HMA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAb16724	NM_001341993.1 Latest version!	Arabidopsis thaliana heavy metal atpase 2 (HMA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HMA2 ( NM_119157.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194740.1 Arabidopsis thaliana heavy metal atpase 2 (HMA2), mRNA.

Price & Availability↑

CloneID	OAb16724
Clone ID Related Accession (Same CDS sequence)	NM_119157.4 , NM_001341993.1
Accession Version	NM_119157.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	heavy metal atpase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_119157.3.

ATGGCGTCGA AGAAGATGAC CAAGAGTTAC TTCGATGTTC TTGGAATTTG CTGTACATCG 
GAGGTTCCGT TGATCGAGAA TATTCTCAAT TCCATGGACG GTGTTAAAGA ATTCTCTGTT 
ATTGTTCCGT CAAGAACCGT CATCGTCGTC CACGACACTC TCATCCTCTC GCAGTTCCAA 
ATCGTTAAAG CACTGAACCA AGCACAGTTA GAAGCAAATG TGAGGGTAAC CGGAGAAACC 
AATTTCAAGA ATAAATGGCC AAGTCCCTTT GCGGTGGTTT CCGGCATACT TCTCCTCCTC 
TCCTTCTTCA AATACCTTTA CTCTCCCTTC CGTTGGCTCG CTGTCGCAGC CGTTGTCGCT 
GGCATTTATC CGATATTAGC CAAAGCTGTA GCATCTCTTG CAAGGTTTAG AATCGACATC 
AACATTCTTG TTGTTGTAAC CGTGGGAGCA ACAATTGGCA TGCAAGATTA CACAGAAGCT 
GCAGTAGTTG TCTTCTTGTT CACAATCGCT GAATGGCTCC AATCTAGAGC TAGCTATAAG 
GCAAGTGCAG TGATGCAGTC TTTGATGAGT TTAGCTCCAC AAAAGGCCGT GATTGCAGAG 
ACTGGAGAAG AAGTTGAAGT GGATGAACTC AAGACTAACA CAGTTATAGC TGTCAAAGCT 
GGTGAAACCA TACCAATTGA TGGAGTTGTT GTTGATGGAA ACTGTGAAGT TGATGAGAAA 
ACTTTGACCG GTGAAGCATT CCCCGTGCCT AAACTGAAAG ATTCGACGGT TTGGGCTGGA 
ACCATCAATC TAAATGGTTA CATAACTGTG AACACCACTG CTCTAGCTGA GGATTGCGTG 
GTTGCGAAGA TGGCAAAACT TGTAGAAGAA GCTCAGAACA GCAAAACCGA AACTCAGAGA 
TTTATAGATA AATGTTCCAA GTACTATACT CCAGCGATCA TCCTGATATC AATCTGTTTT 
GTGGCTATCC CATTTGCATT GAAGGTTCAC AACCTGAAAC ATTGGGTTCA CTTAGCACTA 
GTTGTGTTAG TAAGTGCTTG TCCTTGTGGA CTTATCCTCT CCACACCAGT AGCCACTTTC 
TGTGCACTCA CAAAGGCAGC CACATCAGGA CTTTTGATCA AAGGAGCTGA TTACCTTGAA 
ACCTTAGCCA AGATCAAGAT TGTTGCTTTT GATAAAACCG GGACTATCAC TAGAGGTGAG 
TTCATCGTCA TGGATTTCCA GTCACTTTCT GAAGATATAA GCCTGCAGAG TCTGCTTTAC 
TGGGTATCAA GCACTGAGAG CAAGTCAAGC CATCCAATGG CTGCTGCAGT TGTGGACTAT 
GCAAGATCTG TCTCTGTTGA ACCTAAGCCT GAAGCGGTCG AAGACTACCA GAACTTTCCA 
GGAGAAGGGA TTTATGGGAA GATTGATGGT AAAGAAGTCT ATATCGGTAA CAAAAGGATT 
GCTTCTAGAG CTGGGTGTTT ATCAGTTCCA GATATTGATG TTGATACTAA AGGAGGAAAG 
ACAATTGGAT ATGTATATGT TGGAGAAACA CTAGCTGGTG TTTTCAATCT CTCAGATGCT 
TGTAGATCTG GTGTAGCTCA AGCCATGAAA GAACTGAAAT CGTTGGGAAT TAAGATTGCA 
ATGCTTACCG GAGATAATCA TGCCGCTGCA ATGCATGCTC AAGAACAGCT AGGAAATGCT 
ATGGATATTG TTAGAGCAGA GCTTCTTCCA GAAGATAAAT CTGAAATCAT AAAACAGTTA 
AAGAGAGAAG AAGGGCCAAC GGCTATGGTA GGGGACGGGT TGAATGATGC ACCAGCTTTA 
GCTACAGCGG ATATTGGTAT CTCAATGGGT GTTTCCGGCT CTGCACTTGC AACAGAGACT 
GGTAATATAA TTCTGATGTC CAATGATATC AGAAGAATAC CGCAGGCGAT AAAGCTTGCA 
AAAAGAGCGA AAAGGAAAGT GGTGGAGAAT GTGGTCATAT CAATCACTAT GAAAGGAGCT 
ATTCTTGCAT TGGCATTTGC TGGTCATCCG CTGATTTGGG CAGCTGTTCT TGCAGATGTA 
GGAACTTGTC TGCTTGTGAT CCTTAATAGC ATGTTGTTGC TCAGTGATAA ACATAAAACT 
GGAAACAAAT GTTATAGGGA GTCTTCTTCT TCCTCGGTTT TAATCGCGGA GAAACTTGAA 
GGTGATGCTG CTGGGGACAT GGAAGCAGGC TTATTACCAA AGATCAGTGA TAAGCATTGC 
AAGCCGGGCT GTTGTGGAAC GAAGACTCAA GAGAAAGCGA TGAAACCAGC TAAAGCCAGT 
TCGGACCATA GTCACTCTGG TTGTTGTGAG ACGAAACAGA AAGACAATGT AACGGTTGTG 
AAAAAGAGCT GTTGTGCAGA ACCTGTTGAT TTAGGACATG GCCATGACTC AGGGTGTTGT 
GGTGACAAGA GTCAACAACC ACACCAACAC GAGGTGCAAG TGCAACAAAG CTGTCATAAC 
AAGCCATCTG GCCTGGACTC AGGGTGTTGT GGTGGCAAGA GTCAACAGCC ACACCAACAC 
GAGTTGCAAC AAAGCTGTCA TGACAAGCCA TCTGGCCTGG ACATTGGAAC TGGCCCAAAA 
CACGAAGGTT CTTCAACATT GGTCAATCTG GAGGGGGATG CGAAAGAGGA ACTGAAAGTC 
TTGGTCAATG GTTTTTGTTC AAGTCCTGCT GATCTCGCGA TAACTAGCTT GAAAGTGAAG 
AGCGATAGCC ATTGCAAGTC TAACTGTAGC AGCAGAGAGA GGTGCCACCA TGGCAGCAAC 
TGTTGTAGGA GCTACGCTAA AGAGAGTTGC AGTCACGATC ACCACCACAC AAGGGCTCAT 
GGCGTTGGAA CCTTGAAAGA GATTGTGATT GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194740.1
CDS	11..2866
Translation	MASKKMTKSYFDVLGICCTSEVPLIENILNSMDGVKEFSVIVPSRTVIVVHDTLILSQFQIVKALNQAQLEANVRVTGETNFKNKWPSPFAVVSGILLLLSFFKYLYSPFRWLAVAAVVAGIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEWLQSRASYKASAVMQSLMSLAPQKAVIAETGEEVEVDELKTNTVIAVKAGETIPIDGVVVDGNCEVDEKTLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCVVAKMAKLVEEAQNSKTETQRFIDKCSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVLVSACPCGLILSTPVATFCALTKAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIVMDFQSLSEDISLQSLLYWVSSTESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRIASRAGCLSVPDIDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKSLGIKIAMLTGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREEGPTAMVGDGLNDAPALATADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKGAILALAFAGHPLIWAAVLADVGTCLLVILNSMLLLSDKHKTGNKCYRESSSSSVLIAEKLEGDAAGDMEAGLLPKISDKHCKPGCCGTKTQEKAMKPAKASSDHSHSGCCETKQKDNVTVVKKSCCAEPVDLGHGHDSGCCGDKSQQPHQHEVQVQQSCHNKPSGLDSGCCGGKSQQPHQHELQQSCHDKPSGLDIGTGPKHEGSSTLVNLEGDAKEELKVLVNGFCSSPADLAITSLKVKSDSHCKSNCSSRERCHHGSNCCRSYAKESCSHDHHHTRAHGVGTLKEIVIE

Target ORF information:

RefSeq Version	NM_119157.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana heavy metal atpase 2 (HMA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119157.4

ORF Insert Sequence:

ATGGCGTCGA AGAAGATGAC CAAGAGTTAC TTCGATGTTC TTGGAATTTG CTGTACATCG 
GAGGTTCCGT TGATCGAGAA TATTCTCAAT TCCATGGACG GTGTTAAAGA ATTCTCTGTT 
ATTGTTCCGT CAAGAACCGT CATCGTCGTC CACGACACTC TCATCCTCTC GCAGTTCCAA 
ATCGTTAAAG CACTGAACCA AGCACAGTTA GAAGCAAATG TGAGGGTAAC CGGAGAAACC 
AATTTCAAGA ATAAATGGCC AAGTCCCTTT GCGGTGGTTT CCGGCATACT TCTCCTCCTC 
TCCTTCTTCA AATACCTTTA CTCTCCCTTC CGTTGGCTCG CTGTCGCAGC CGTTGTCGCT 
GGCATTTATC CGATATTAGC CAAAGCTGTA GCATCTCTTG CAAGGTTTAG AATCGACATC 
AACATTCTTG TTGTTGTAAC CGTGGGAGCA ACAATTGGCA TGCAAGATTA CACAGAAGCT 
GCAGTAGTTG TCTTCTTGTT CACAATCGCT GAATGGCTCC AATCTAGAGC TAGCTATAAG 
GCAAGTGCAG TGATGCAGTC TTTGATGAGT TTAGCTCCAC AAAAGGCCGT GATTGCAGAG 
ACTGGAGAAG AAGTTGAAGT GGATGAACTC AAGACTAACA CAGTTATAGC TGTCAAAGCT 
GGTGAAACCA TACCAATTGA TGGAGTTGTT GTTGATGGAA ACTGTGAAGT TGATGAGAAA 
ACTTTGACCG GTGAAGCATT CCCCGTGCCT AAACTGAAAG ATTCGACGGT TTGGGCTGGA 
ACCATCAATC TAAATGGTTA CATAACTGTG AACACCACTG CTCTAGCTGA GGATTGCGTG 
GTTGCGAAGA TGGCAAAACT TGTAGAAGAA GCTCAGAACA GCAAAACCGA AACTCAGAGA 
TTTATAGATA AATGTTCCAA GTACTATACT CCAGCGATCA TCCTGATATC AATCTGTTTT 
GTGGCTATCC CATTTGCATT GAAGGTTCAC AACCTGAAAC ATTGGGTTCA CTTAGCACTA 
GTTGTGTTAG TAAGTGCTTG TCCTTGTGGA CTTATCCTCT CCACACCAGT AGCCACTTTC 
TGTGCACTCA CAAAGGCAGC CACATCAGGA CTTTTGATCA AAGGAGCTGA TTACCTTGAA 
ACCTTAGCCA AGATCAAGAT TGTTGCTTTT GATAAAACCG GGACTATCAC TAGAGGTGAG 
TTCATCGTCA TGGATTTCCA GTCACTTTCT GAAGATATAA GCCTGCAGAG TCTGCTTTAC 
TGGGTATCAA GCACTGAGAG CAAGTCAAGC CATCCAATGG CTGCTGCAGT TGTGGACTAT 
GCAAGATCTG TCTCTGTTGA ACCTAAGCCT GAAGCGGTCG AAGACTACCA GAACTTTCCA 
GGAGAAGGGA TTTATGGGAA GATTGATGGT AAAGAAGTCT ATATCGGTAA CAAAAGGATT 
GCTTCTAGAG CTGGGTGTTT ATCAGTTCCA GATATTGATG TTGATACTAA AGGAGGAAAG 
ACAATTGGAT ATGTATATGT TGGAGAAACA CTAGCTGGTG TTTTCAATCT CTCAGATGCT 
TGTAGATCTG GTGTAGCTCA AGCCATGAAA GAACTGAAAT CGTTGGGAAT TAAGATTGCA 
ATGCTTACCG GAGATAATCA TGCCGCTGCA ATGCATGCTC AAGAACAGCT AGGAAATGCT 
ATGGATATTG TTAGAGCAGA GCTTCTTCCA GAAGATAAAT CTGAAATCAT AAAACAGTTA 
AAGAGAGAAG AAGGGCCAAC GGCTATGGTA GGGGACGGGT TGAATGATGC ACCAGCTTTA 
GCTACAGCGG ATATTGGTAT CTCAATGGGT GTTTCCGGCT CTGCACTTGC AACAGAGACT 
GGTAATATAA TTCTGATGTC CAATGATATC AGAAGAATAC CGCAGGCGAT AAAGCTTGCA 
AAAAGAGCGA AAAGGAAAGT GGTGGAGAAT GTGGTCATAT CAATCACTAT GAAAGGAGCT 
ATTCTTGCAT TGGCATTTGC TGGTCATCCG CTGATTTGGG CAGCTGTTCT TGCAGATGTA 
GGAACTTGTC TGCTTGTGAT CCTTAATAGC ATGTTGTTGC TCAGTGATAA ACATAAAACT 
GGAAACAAAT GTTATAGGGA GTCTTCTTCT TCCTCGGTTT TAATCGCGGA GAAACTTGAA 
GGTGATGCTG CTGGGGACAT GGAAGCAGGC TTATTACCAA AGATCAGTGA TAAGCATTGC 
AAGCCGGGCT GTTGTGGAAC GAAGACTCAA GAGAAAGCGA TGAAACCAGC TAAAGCCAGT 
TCGGACCATA GTCACTCTGG TTGTTGTGAG ACGAAACAGA AAGACAATGT AACGGTTGTG 
AAAAAGAGCT GTTGTGCAGA ACCTGTTGAT TTAGGACATG GCCATGACTC AGGGTGTTGT 
GGTGACAAGA GTCAACAACC ACACCAACAC GAGGTGCAAG TGCAACAAAG CTGTCATAAC 
AAGCCATCTG GCCTGGACTC AGGGTGTTGT GGTGGCAAGA GTCAACAGCC ACACCAACAC 
GAGTTGCAAC AAAGCTGTCA TGACAAGCCA TCTGGCCTGG ACATTGGAAC TGGCCCAAAA 
CACGAAGGTT CTTCAACATT GGTCAATCTG GAGGGGGATG CGAAAGAGGA ACTGAAAGTC 
TTGGTCAATG GTTTTTGTTC AAGTCCTGCT GATCTCGCGA TAACTAGCTT GAAAGTGAAG 
AGCGATAGCC ATTGCAAGTC TAACTGTAGC AGCAGAGAGA GGTGCCACCA TGGCAGCAAC 
TGTTGTAGGA GCTACGCTAA AGAGAGTTGC AGTCACGATC ACCACCACAC AAGGGCTCAT 
GGCGTTGGAA CCTTGAAAGA GATTGTGATT GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMA2 ( NM_001341993.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001320088.1 Arabidopsis thaliana heavy metal atpase 2 (HMA2), mRNA.

Price & Availability↑

CloneID	OAb16724
Clone ID Related Accession (Same CDS sequence)	NM_119157.4 , NM_001341993.1
Accession Version	NM_001341993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	heavy metal atpase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGCGTCGA AGAAGATGAC CAAGAGTTAC TTCGATGTTC TTGGAATTTG CTGTACATCG 
GAGGTTCCGT TGATCGAGAA TATTCTCAAT TCCATGGACG GTGTTAAAGA ATTCTCTGTT 
ATTGTTCCGT CAAGAACCGT CATCGTCGTC CACGACACTC TCATCCTCTC GCAGTTCCAA 
ATCGTTAAAG CACTGAACCA AGCACAGTTA GAAGCAAATG TGAGGGTAAC CGGAGAAACC 
AATTTCAAGA ATAAATGGCC AAGTCCCTTT GCGGTGGTTT CCGGCATACT TCTCCTCCTC 
TCCTTCTTCA AATACCTTTA CTCTCCCTTC CGTTGGCTCG CTGTCGCAGC CGTTGTCGCT 
GGCATTTATC CGATATTAGC CAAAGCTGTA GCATCTCTTG CAAGGTTTAG AATCGACATC 
AACATTCTTG TTGTTGTAAC CGTGGGAGCA ACAATTGGCA TGCAAGATTA CACAGAAGCT 
GCAGTAGTTG TCTTCTTGTT CACAATCGCT GAATGGCTCC AATCTAGAGC TAGCTATAAG 
GCAAGTGCAG TGATGCAGTC TTTGATGAGT TTAGCTCCAC AAAAGGCCGT GATTGCAGAG 
ACTGGAGAAG AAGTTGAAGT GGATGAACTC AAGACTAACA CAGTTATAGC TGTCAAAGCT 
GGTGAAACCA TACCAATTGA TGGAGTTGTT GTTGATGGAA ACTGTGAAGT TGATGAGAAA 
ACTTTGACCG GTGAAGCATT CCCCGTGCCT AAACTGAAAG ATTCGACGGT TTGGGCTGGA 
ACCATCAATC TAAATGGTTA CATAACTGTG AACACCACTG CTCTAGCTGA GGATTGCGTG 
GTTGCGAAGA TGGCAAAACT TGTAGAAGAA GCTCAGAACA GCAAAACCGA AACTCAGAGA 
TTTATAGATA AATGTTCCAA GTACTATACT CCAGCGATCA TCCTGATATC AATCTGTTTT 
GTGGCTATCC CATTTGCATT GAAGGTTCAC AACCTGAAAC ATTGGGTTCA CTTAGCACTA 
GTTGTGTTAG TAAGTGCTTG TCCTTGTGGA CTTATCCTCT CCACACCAGT AGCCACTTTC 
TGTGCACTCA CAAAGGCAGC CACATCAGGA CTTTTGATCA AAGGAGCTGA TTACCTTGAA 
ACCTTAGCCA AGATCAAGAT TGTTGCTTTT GATAAAACCG GGACTATCAC TAGAGGTGAG 
TTCATCGTCA TGGATTTCCA GTCACTTTCT GAAGATATAA GCCTGCAGAG TCTGCTTTAC 
TGGGTATCAA GCACTGAGAG CAAGTCAAGC CATCCAATGG CTGCTGCAGT TGTGGACTAT 
GCAAGATCTG TCTCTGTTGA ACCTAAGCCT GAAGCGGTCG AAGACTACCA GAACTTTCCA 
GGAGAAGGGA TTTATGGGAA GATTGATGGT AAAGAAGTCT ATATCGGTAA CAAAAGGATT 
GCTTCTAGAG CTGGGTGTTT ATCAGTTCCA GATATTGATG TTGATACTAA AGGAGGAAAG 
ACAATTGGAT ATGTATATGT TGGAGAAACA CTAGCTGGTG TTTTCAATCT CTCAGATGCT 
TGTAGATCTG GTGTAGCTCA AGCCATGAAA GAACTGAAAT CGTTGGGAAT TAAGATTGCA 
ATGCTTACCG GAGATAATCA TGCCGCTGCA ATGCATGCTC AAGAACAGCT AGGAAATGCT 
ATGGATATTG TTAGAGCAGA GCTTCTTCCA GAAGATAAAT CTGAAATCAT AAAACAGTTA 
AAGAGAGAAG AAGGGCCAAC GGCTATGGTA GGGGACGGGT TGAATGATGC ACCAGCTTTA 
GCTACAGCGG ATATTGGTAT CTCAATGGGT GTTTCCGGCT CTGCACTTGC AACAGAGACT 
GGTAATATAA TTCTGATGTC CAATGATATC AGAAGAATAC CGCAGGCGAT AAAGCTTGCA 
AAAAGAGCGA AAAGGAAAGT GGTGGAGAAT GTGGTCATAT CAATCACTAT GAAAGGAGCT 
ATTCTTGCAT TGGCATTTGC TGGTCATCCG CTGATTTGGG CAGCTGTTCT TGCAGATGTA 
GGAACTTGTC TGCTTGTGAT CCTTAATAGC ATGTTGTTGC TCAGTGATAA ACATAAAACT 
GGAAACAAAT GTTATAGGGA GTCTTCTTCT TCCTCGGTTT TAATCGCGGA GAAACTTGAA 
GGTGATGCTG CTGGGGACAT GGAAGCAGGC TTATTACCAA AGATCAGTGA TAAGCATTGC 
AAGCCGGGCT GTTGTGGAAC GAAGACTCAA GAGAAAGCGA TGAAACCAGC TAAAGCCAGT 
TCGGACCATA GTCACTCTGG TTGTTGTGAG ACGAAACAGA AAGACAATGT AACGGTTGTG 
AAAAAGAGCT GTTGTGCAGA ACCTGTTGAT TTAGGACATG GCCATGACTC AGGGTGTTGT 
GGTGACAAGA GTCAACAACC ACACCAACAC GAGGTGCAAG TGCAACAAAG CTGTCATAAC 
AAGCCATCTG GCCTGGACTC AGGGTGTTGT GGTGGCAAGA GTCAACAGCC ACACCAACAC 
GAGTTGCAAC AAAGCTGTCA TGACAAGCCA TCTGGCCTGG ACATTGGAAC TGGCCCAAAA 
CACGAAGGTT CTTCAACATT GGTCAATCTG GAGGGGGATG CGAAAGAGGA ACTGAAAGTC 
TTGGTCAATG GTTTTTGTTC AAGTCCTGCT GATCTCGCGA TAACTAGCTT GAAAGTGAAG 
AGCGATAGCC ATTGCAAGTC TAACTGTAGC AGCAGAGAGA GGTGCCACCA TGGCAGCAAC 
TGTTGTAGGA GCTACGCTAA AGAGAGTTGC AGTCACGATC ACCACCACAC AAGGGCTCAT 
GGCGTTGGAA CCTTGAAAGA GATTGTGATT GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001320088.1
CDS	156..3011
Translation	MASKKMTKSYFDVLGICCTSEVPLIENILNSMDGVKEFSVIVPSRTVIVVHDTLILSQFQIVKALNQAQLEANVRVTGETNFKNKWPSPFAVVSGILLLLSFFKYLYSPFRWLAVAAVVAGIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEWLQSRASYKASAVMQSLMSLAPQKAVIAETGEEVEVDELKTNTVIAVKAGETIPIDGVVVDGNCEVDEKTLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCVVAKMAKLVEEAQNSKTETQRFIDKCSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVLVSACPCGLILSTPVATFCALTKAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIVMDFQSLSEDISLQSLLYWVSSTESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRIASRAGCLSVPDIDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKSLGIKIAMLTGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREEGPTAMVGDGLNDAPALATADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKGAILALAFAGHPLIWAAVLADVGTCLLVILNSMLLLSDKHKTGNKCYRESSSSSVLIAEKLEGDAAGDMEAGLLPKISDKHCKPGCCGTKTQEKAMKPAKASSDHSHSGCCETKQKDNVTVVKKSCCAEPVDLGHGHDSGCCGDKSQQPHQHEVQVQQSCHNKPSGLDSGCCGGKSQQPHQHELQQSCHDKPSGLDIGTGPKHEGSSTLVNLEGDAKEELKVLVNGFCSSPADLAITSLKVKSDSHCKSNCSSRERCHHGSNCCRSYAKESCSHDHHHTRAHGVGTLKEIVIE

Target ORF information:

RefSeq Version	NM_001341993.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana heavy metal atpase 2 (HMA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341993.1

ORF Insert Sequence:

ATGGCGTCGA AGAAGATGAC CAAGAGTTAC TTCGATGTTC TTGGAATTTG CTGTACATCG 
GAGGTTCCGT TGATCGAGAA TATTCTCAAT TCCATGGACG GTGTTAAAGA ATTCTCTGTT 
ATTGTTCCGT CAAGAACCGT CATCGTCGTC CACGACACTC TCATCCTCTC GCAGTTCCAA 
ATCGTTAAAG CACTGAACCA AGCACAGTTA GAAGCAAATG TGAGGGTAAC CGGAGAAACC 
AATTTCAAGA ATAAATGGCC AAGTCCCTTT GCGGTGGTTT CCGGCATACT TCTCCTCCTC 
TCCTTCTTCA AATACCTTTA CTCTCCCTTC CGTTGGCTCG CTGTCGCAGC CGTTGTCGCT 
GGCATTTATC CGATATTAGC CAAAGCTGTA GCATCTCTTG CAAGGTTTAG AATCGACATC 
AACATTCTTG TTGTTGTAAC CGTGGGAGCA ACAATTGGCA TGCAAGATTA CACAGAAGCT 
GCAGTAGTTG TCTTCTTGTT CACAATCGCT GAATGGCTCC AATCTAGAGC TAGCTATAAG 
GCAAGTGCAG TGATGCAGTC TTTGATGAGT TTAGCTCCAC AAAAGGCCGT GATTGCAGAG 
ACTGGAGAAG AAGTTGAAGT GGATGAACTC AAGACTAACA CAGTTATAGC TGTCAAAGCT 
GGTGAAACCA TACCAATTGA TGGAGTTGTT GTTGATGGAA ACTGTGAAGT TGATGAGAAA 
ACTTTGACCG GTGAAGCATT CCCCGTGCCT AAACTGAAAG ATTCGACGGT TTGGGCTGGA 
ACCATCAATC TAAATGGTTA CATAACTGTG AACACCACTG CTCTAGCTGA GGATTGCGTG 
GTTGCGAAGA TGGCAAAACT TGTAGAAGAA GCTCAGAACA GCAAAACCGA AACTCAGAGA 
TTTATAGATA AATGTTCCAA GTACTATACT CCAGCGATCA TCCTGATATC AATCTGTTTT 
GTGGCTATCC CATTTGCATT GAAGGTTCAC AACCTGAAAC ATTGGGTTCA CTTAGCACTA 
GTTGTGTTAG TAAGTGCTTG TCCTTGTGGA CTTATCCTCT CCACACCAGT AGCCACTTTC 
TGTGCACTCA CAAAGGCAGC CACATCAGGA CTTTTGATCA AAGGAGCTGA TTACCTTGAA 
ACCTTAGCCA AGATCAAGAT TGTTGCTTTT GATAAAACCG GGACTATCAC TAGAGGTGAG 
TTCATCGTCA TGGATTTCCA GTCACTTTCT GAAGATATAA GCCTGCAGAG TCTGCTTTAC 
TGGGTATCAA GCACTGAGAG CAAGTCAAGC CATCCAATGG CTGCTGCAGT TGTGGACTAT 
GCAAGATCTG TCTCTGTTGA ACCTAAGCCT GAAGCGGTCG AAGACTACCA GAACTTTCCA 
GGAGAAGGGA TTTATGGGAA GATTGATGGT AAAGAAGTCT ATATCGGTAA CAAAAGGATT 
GCTTCTAGAG CTGGGTGTTT ATCAGTTCCA GATATTGATG TTGATACTAA AGGAGGAAAG 
ACAATTGGAT ATGTATATGT TGGAGAAACA CTAGCTGGTG TTTTCAATCT CTCAGATGCT 
TGTAGATCTG GTGTAGCTCA AGCCATGAAA GAACTGAAAT CGTTGGGAAT TAAGATTGCA 
ATGCTTACCG GAGATAATCA TGCCGCTGCA ATGCATGCTC AAGAACAGCT AGGAAATGCT 
ATGGATATTG TTAGAGCAGA GCTTCTTCCA GAAGATAAAT CTGAAATCAT AAAACAGTTA 
AAGAGAGAAG AAGGGCCAAC GGCTATGGTA GGGGACGGGT TGAATGATGC ACCAGCTTTA 
GCTACAGCGG ATATTGGTAT CTCAATGGGT GTTTCCGGCT CTGCACTTGC AACAGAGACT 
GGTAATATAA TTCTGATGTC CAATGATATC AGAAGAATAC CGCAGGCGAT AAAGCTTGCA 
AAAAGAGCGA AAAGGAAAGT GGTGGAGAAT GTGGTCATAT CAATCACTAT GAAAGGAGCT 
ATTCTTGCAT TGGCATTTGC TGGTCATCCG CTGATTTGGG CAGCTGTTCT TGCAGATGTA 
GGAACTTGTC TGCTTGTGAT CCTTAATAGC ATGTTGTTGC TCAGTGATAA ACATAAAACT 
GGAAACAAAT GTTATAGGGA GTCTTCTTCT TCCTCGGTTT TAATCGCGGA GAAACTTGAA 
GGTGATGCTG CTGGGGACAT GGAAGCAGGC TTATTACCAA AGATCAGTGA TAAGCATTGC 
AAGCCGGGCT GTTGTGGAAC GAAGACTCAA GAGAAAGCGA TGAAACCAGC TAAAGCCAGT 
TCGGACCATA GTCACTCTGG TTGTTGTGAG ACGAAACAGA AAGACAATGT AACGGTTGTG 
AAAAAGAGCT GTTGTGCAGA ACCTGTTGAT TTAGGACATG GCCATGACTC AGGGTGTTGT 
GGTGACAAGA GTCAACAACC ACACCAACAC GAGGTGCAAG TGCAACAAAG CTGTCATAAC 
AAGCCATCTG GCCTGGACTC AGGGTGTTGT GGTGGCAAGA GTCAACAGCC ACACCAACAC 
GAGTTGCAAC AAAGCTGTCA TGACAAGCCA TCTGGCCTGG ACATTGGAAC TGGCCCAAAA 
CACGAAGGTT CTTCAACATT GGTCAATCTG GAGGGGGATG CGAAAGAGGA ACTGAAAGTC 
TTGGTCAATG GTTTTTGTTC AAGTCCTGCT GATCTCGCGA TAACTAGCTT GAAAGTGAAG 
AGCGATAGCC ATTGCAAGTC TAACTGTAGC AGCAGAGAGA GGTGCCACCA TGGCAGCAAC 
TGTTGTAGGA GCTACGCTAA AGAGAGTTGC AGTCACGATC ACCACCACAC AAGGGCTCAT 
GGCGTTGGAA CCTTGAAAGA GATTGTGATT GAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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HMA2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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