AT4G25090 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT4G25090
Entrez Gene ID	828612
Full Name	Riboflavin synthase-like superfamily protein
Synonyms	F24A6.3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_118641.3	NP_194239.2	Riboflavin synthase-like superfamily protein
NM_001203904.1	NP_001190833.1	Riboflavin synthase-like superfamily protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DUOX1	XP_001163196.1
Arabidopsis thaliana (thale cress)	RBOHD	NP_199602.1
Arabidopsis thaliana (thale cress)	AT4G25090	NP_194239.2
Mus musculus (house mouse)	Duox1	NP_001092767.1
Danio rerio (zebrafish)	duox	XP_001919394.4
Arabidopsis thaliana (thale cress)	RBOHA	NP_196356.1
Oryza sativa (rice)	Os01g0360200	NP_001043020.1
Rattus norvegicus (Norway rat)	Duox1	NP_714961.1
Xenopus tropicalis (tropical clawed frog)	duox1	XP_002937936.2
	DUOX1	XP_001111370.1
Canis lupus familiaris (dog)	DUOX1	NP_001003122.1
Gallus gallus (chicken)	DUOX2	XP_425053.4
Homo sapiens (human)	DUOX1	NP_059130.2
Bos taurus (cattle)	DUOX1	NP_001192603.1
Arabidopsis thaliana (thale cress)	RHD2	NP_199919.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT4G25090 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT4G25090 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16222	NM_118641.3 Latest version!	Arabidopsis thaliana Riboflavin synthase-like superfamily protein (AT4G25090), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb32473	NM_001203904.1 Latest version!	Arabidopsis thaliana Riboflavin synthase-like superfamily protein (AT4G25090), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT4G25090 ( NM_118641.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194239.2 Arabidopsis thaliana Riboflavin synthase-like superfamily protein (AT4G25090), mRNA.

Price & Availability↑

CloneID	OAb16222
Clone ID Related Accession (Same CDS sequence)	NM_118641.3
Accession Version	NM_118641.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Riboflavin synthase-like superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118641.2.

ATGCAGAGAG TGAGCTTTGA AGTAAAGGAC ACAGAGGCGG AGAAGAGCAG CAGCGAGATA 
CTGAGCGGAT CTTTGCCGTC GACCTACCGA AATCCGGCGA TGGAAAACGT TGGAAACGCG 
GTGGATGATG GATCGAGCGT GAAGAACAAT CCGAAACTTG ATATGCAGAA ACAGAACGGT 
TTGGTGAAGT GGTTCAAGAA GTGCTTGACA ATGGTATCTG GAGAGAGTAA GGCACCTCGA 
TTGGACCGGT CGAAGTCAAC GGCCGGTCAG GCCTTGAAAG GGCTCAAGAT CATCAGTAAG 
ACCGACGGTA ACGCTGCCTG GACCGTCGTG GAGAAGCGGT ACTTGAAGAT CACGGCCAAT 
ACTGATGGAT TGCTGCTTCG GTCGAAATTC GGTGAATGCA TAGGGATGAA CTCAAAAGAA 
TTTGCCTTGG AATTGTTTGA CGCCTTAGCT AGAAAAAGTC ACTTGAAAGG GGATGTGATT 
ACTGAGACCG AGTTGAAGAA ATTTTGGGAA CAAATAAATG ATAAAAGCTT TGATTCAAGG 
CTTATCACGT TCTTTGACTT GATGGATAAA GACTCGGATG GTAGACTTAC GGAAGACGAA 
GTTAGAGAGA TCATCAAGCT TAGTTCTTCT GCCAATCATC TGTCATGCAT CCAAAACAAG 
GCAGATGAAT ATGCAGCAAT GATAATGGAA GAGCTTGACC CTGATCATAT GGGATACATC 
ATGATGGAGA GTCTAAAGAA ATTGCTTCTG CAAGCGGAAA CAAAATCTGT AAGCACAGAT 
ATTAATAGTG AAGAAAGAAA GGAGCTGAGC GACATGCTAA CTGAGAGTCT CAAGCCTACA 
CGTGACCCTA ACCATTTGAG GAGATGGTAT TGTCAACTAA GATTCTTTGT ATTGGATAGT 
TGGCAAAGAG TTTGGGTGAT AGCGTTATGG CTCACGATTA TGGCCATCCT CTTCGCGTAC 
AAGTATATTC AATACAAGAA TAGAGCAGTG TATGAAGTGT TGGGGCCTTG TGTGTGCTTG 
GCTAAAGGTG CAGCAGAGAC GCTGAAGCTA AACATGGCCC TAATTCTGTT ACCTGTTTGC 
AGAAATACCA TCACATGGCT TAGAAATAAG ACTAGGTTGG GTGTTTTTGT TCCTTTTGAT 
GACAACCTCA ATTTTCACAA GGTTATAGCG GTGGGAATTG CGATAGGAGT AGCCATACAC 
TCTGTGTCCC ACTTGGCGTG TGATTTTCCA CTGCTAATCG CGGCAACTCC AGCGGAATAC 
ATGCCTCTCG GAAAATTCTT CGGAGAGGAG CAACCAAAGA GATACTTGCA TTTTGTGAAG 
TCAACAGAAG GAATAACAGG GCTTGTAATG GTTTTCTTGA TGGTTATTGC TTTTACACTG 
GCTATGCCTT GGTTTAGACG GGGGAAGCTA GAGAAAAAGC TACCAGGGCC ACTAAAGAAA 
CTTGCGAGCT TCAATGCCTT CTGGTACACT CACCATTTGT TCGTTATAGT CTACATTCTC 
CTTGTCCTTC ACGGATACTA CATATATCTC AACAAGGAAT GGTACAAGAA AACGACGTGG 
ATGTACTTGG CAGTACCAGT AGCTCTTTAT GCATACGAGA GATTGATACG AGCATTCAGG 
TCCAGCATCA GGACAGTAAA AGTCCTTAAG ATGGCAGCTT ATCCTGGAAA AGTATTGACT 
TTGCAGATGT CAAAGCCTAC AAACTTCAAA TACATGAGCG GCCAATACAT GTTTGTAAAT 
TGTCCAGCAG TTTCACCGTT TGAGTGGCAT CCATTTTCAA TAACTTCTAC ACCACAAGAC 
GACTATCTGA GCGTTCATAT AAAAGCATTG GGAGATTGGA CAGAGGCTAT CCAAGGAGTT 
TTCTCAGAGG TGTCTAAGCC ACCTCCGGTC GGAGACATGT TGAATGGTGC AAATAGTCCC 
CGTTTCCCCA AAATTATGAT TGATGGTCCG TACGGCGCAC CAGCTCAAGA CTACAAGAAG 
TACGAGGTGG TCCTGCTGAT AGGTCTTGGC ATTGGAGCCA CCCCGATGAT CAGCATTATC 
AAGGACATTA TTAACAATAC GGAGACCAAG GAACAACTCA GCCAAATGGA GAAGGGATCG 
CCACAAGAGC AACAAGGTAA TAAAGAAACT TTCAAGACTC GAAGGGCTTA CTTCTACTGG 
GTAACAAAGG AGCAGGGCAC ATTTGATTGG TTCAAGAACA TCATGAACGA GATCGCAGAA 
CGGGACAAAA GTAAAGTCAT AGAACTACAC AATCATTGCA CTAGTGTCTA CGAAGAAGGA 
GACGTTCGTT CCGCGCTCAT ACGGATGCTT CAGTCTCTTA ATTATGCCAA GAATGGTTTA 
GACATTGTTG CTGGGACAAG GGTCATGTCC CATTTCGCCC GACCTAATTG GAAAAACGTC 
TACAAACAGA TAGCCATGGA TCACCCTGGC GCTAATGTTG GAGTGTTCTA TTGTGGAGCA 
CCGGTATTGA CGAAGGAGTT AAGGCAATTA GCTTTGGAAT TCACACACAA GACCAGTACT 
AGATTCTCTT TCCACAAAGA GAACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194239.2
CDS	207..2756
Translation	MQRVSFEVKDTEAEKSSSEILSGSLPSTYRNPAMENVGNAVDDGSSVKNNPKLDMQKQNGLVKWFKKCLTMVSGESKAPRLDRSKSTAGQALKGLKIISKTDGNAAWTVVEKRYLKITANTDGLLLRSKFGECIGMNSKEFALELFDALARKSHLKGDVITETELKKFWEQINDKSFDSRLITFFDLMDKDSDGRLTEDEVREIIKLSSSANHLSCIQNKADEYAAMIMEELDPDHMGYIMMESLKKLLLQAETKSVSTDINSEERKELSDMLTESLKPTRDPNHLRRWYCQLRFFVLDSWQRVWVIALWLTIMAILFAYKYIQYKNRAVYEVLGPCVCLAKGAAETLKLNMALILLPVCRNTITWLRNKTRLGVFVPFDDNLNFHKVIAVGIAIGVAIHSVSHLACDFPLLIAATPAEYMPLGKFFGEEQPKRYLHFVKSTEGITGLVMVFLMVIAFTLAMPWFRRGKLEKKLPGPLKKLASFNAFWYTHHLFVIVYILLVLHGYYIYLNKEWYKKTTWMYLAVPVALYAYERLIRAFRSSIRTVKVLKMAAYPGKVLTLQMSKPTNFKYMSGQYMFVNCPAVSPFEWHPFSITSTPQDDYLSVHIKALGDWTEAIQGVFSEVSKPPPVGDMLNGANSPRFPKIMIDGPYGAPAQDYKKYEVVLLIGLGIGATPMISIIKDIINNTETKEQLSQMEKGSPQEQQGNKETFKTRRAYFYWVTKEQGTFDWFKNIMNEIAERDKSKVIELHNHCTSVYEEGDVRSALIRMLQSLNYAKNGLDIVAGTRVMSHFARPNWKNVYKQIAMDHPGANVGVFYCGAPVLTKELRQLALEFTHKTSTRFSFHKENF

Target ORF information:

RefSeq Version	NM_118641.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Riboflavin synthase-like superfamily protein (AT4G25090), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118641.3

ORF Insert Sequence:

ATGCAGAGAG TGAGCTTTGA AGTAAAGGAC ACAGAGGCGG AGAAGAGCAG CAGCGAGATA 
CTGAGCGGAT CTTTGCCGTC GACCTACCGA AATCCGGCGA TGGAAAACGT TGGAAACGCG 
GTGGATGATG GATCGAGCGT GAAGAACAAT CCGAAACTTG ATATGCAGAA ACAGAACGGT 
TTGGTGAAGT GGTTCAAGAA GTGCTTGACA ATGGTATCTG GAGAGAGTAA GGCACCTCGA 
TTGGACCGGT CGAAGTCAAC GGCCGGTCAG GCCTTGAAAG GGCTCAAGAT CATCAGTAAG 
ACCGACGGTA ACGCTGCCTG GACCGTCGTG GAGAAGCGGT ACTTGAAGAT CACGGCCAAT 
ACTGATGGAT TGCTGCTTCG GTCGAAATTC GGTGAATGCA TAGGGATGAA CTCAAAAGAA 
TTTGCCTTGG AATTGTTTGA CGCCTTAGCT AGAAAAAGTC ACTTGAAAGG GGATGTGATT 
ACTGAGACCG AGTTGAAGAA ATTTTGGGAA CAAATAAATG ATAAAAGCTT TGATTCAAGG 
CTTATCACGT TCTTTGACTT GATGGATAAA GACTCGGATG GTAGACTTAC GGAAGACGAA 
GTTAGAGAGA TCATCAAGCT TAGTTCTTCT GCCAATCATC TGTCATGCAT CCAAAACAAG 
GCAGATGAAT ATGCAGCAAT GATAATGGAA GAGCTTGACC CTGATCATAT GGGATACATC 
ATGATGGAGA GTCTAAAGAA ATTGCTTCTG CAAGCGGAAA CAAAATCTGT AAGCACAGAT 
ATTAATAGTG AAGAAAGAAA GGAGCTGAGC GACATGCTAA CTGAGAGTCT CAAGCCTACA 
CGTGACCCTA ACCATTTGAG GAGATGGTAT TGTCAACTAA GATTCTTTGT ATTGGATAGT 
TGGCAAAGAG TTTGGGTGAT AGCGTTATGG CTCACGATTA TGGCCATCCT CTTCGCGTAC 
AAGTATATTC AATACAAGAA TAGAGCAGTG TATGAAGTGT TGGGGCCTTG TGTGTGCTTG 
GCTAAAGGTG CAGCAGAGAC GCTGAAGCTA AACATGGCCC TAATTCTGTT ACCTGTTTGC 
AGAAATACCA TCACATGGCT TAGAAATAAG ACTAGGTTGG GTGTTTTTGT TCCTTTTGAT 
GACAACCTCA ATTTTCACAA GGTTATAGCG GTGGGAATTG CGATAGGAGT AGCCATACAC 
TCTGTGTCCC ACTTGGCGTG TGATTTTCCA CTGCTAATCG CGGCAACTCC AGCGGAATAC 
ATGCCTCTCG GAAAATTCTT CGGAGAGGAG CAACCAAAGA GATACTTGCA TTTTGTGAAG 
TCAACAGAAG GAATAACAGG GCTTGTAATG GTTTTCTTGA TGGTTATTGC TTTTACACTG 
GCTATGCCTT GGTTTAGACG GGGGAAGCTA GAGAAAAAGC TACCAGGGCC ACTAAAGAAA 
CTTGCGAGCT TCAATGCCTT CTGGTACACT CACCATTTGT TCGTTATAGT CTACATTCTC 
CTTGTCCTTC ACGGATACTA CATATATCTC AACAAGGAAT GGTACAAGAA AACGACGTGG 
ATGTACTTGG CAGTACCAGT AGCTCTTTAT GCATACGAGA GATTGATACG AGCATTCAGG 
TCCAGCATCA GGACAGTAAA AGTCCTTAAG ATGGCAGCTT ATCCTGGAAA AGTATTGACT 
TTGCAGATGT CAAAGCCTAC AAACTTCAAA TACATGAGCG GCCAATACAT GTTTGTAAAT 
TGTCCAGCAG TTTCACCGTT TGAGTGGCAT CCATTTTCAA TAACTTCTAC ACCACAAGAC 
GACTATCTGA GCGTTCATAT AAAAGCATTG GGAGATTGGA CAGAGGCTAT CCAAGGAGTT 
TTCTCAGAGG TGTCTAAGCC ACCTCCGGTC GGAGACATGT TGAATGGTGC AAATAGTCCC 
CGTTTCCCCA AAATTATGAT TGATGGTCCG TACGGCGCAC CAGCTCAAGA CTACAAGAAG 
TACGAGGTGG TCCTGCTGAT AGGTCTTGGC ATTGGAGCCA CCCCGATGAT CAGCATTATC 
AAGGACATTA TTAACAATAC GGAGACCAAG GAACAACTCA GCCAAATGGA GAAGGGATCG 
CCACAAGAGC AACAAGGTAA TAAAGAAACT TTCAAGACTC GAAGGGCTTA CTTCTACTGG 
GTAACAAAGG AGCAGGGCAC ATTTGATTGG TTCAAGAACA TCATGAACGA GATCGCAGAA 
CGGGACAAAA GTAAAGTCAT AGAACTACAC AATCATTGCA CTAGTGTCTA CGAAGAAGGA 
GACGTTCGTT CCGCGCTCAT ACGGATGCTT CAGTCTCTTA ATTATGCCAA GAATGGTTTA 
GACATTGTTG CTGGGACAAG GGTCATGTCC CATTTCGCCC GACCTAATTG GAAAAACGTC 
TACAAACAGA TAGCCATGGA TCACCCTGGC GCTAATGTTG GAGTGTTCTA TTGTGGAGCA 
CCGGTATTGA CGAAGGAGTT AAGGCAATTA GCTTTGGAAT TCACACACAA GACCAGTACT 
AGATTCTCTT TCCACAAAGA GAACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT4G25090 ( NM_001203904.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001190833.1 Arabidopsis thaliana Riboflavin synthase-like superfamily protein (AT4G25090), mRNA.

Price & Availability↑

CloneID	OAb32473
Clone ID Related Accession (Same CDS sequence)	NM_001203904.1
Accession Version	NM_001203904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Riboflavin synthase-like superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGCAGAGAG TGAGCTTTGA AGTAAAGGAC ACAGAGGCGG AGAAGAGCAG CAGCGAGATA 
CTGAGCGGAT CTTTGCCGTC GACCTACCGA AATCCGGCGA TGGAAAACGT TGGAAACGCG 
GTGGATGATG GATCGAGCGT GAAGAACAAT CCGAAACTTG ATATGCAGAA ACAGAACGGT 
TTGGTGAAGT GGTTCAAGAA GTGCTTGACA ATGGTATCTG GAGAGAGTAA GGCACCTCGA 
TTGGACCGGT CGAAGTCAAC GGCCGGTCAG GCCTTGAAAG GGCTCAAGAT CATCAGTAAG 
ACCGACGGTA ACGCTGCCTG GACCGTCGTG GAGAAGCGGT ACTTGAAGAT CACGGCCAAT 
ACTGATGGAT TGCTGCTTCG GTCGAAATTC GGTGAATGCA TAGGGATGAA CTCAAAAGAA 
TTTGCCTTGG AATTGTTTGA CGCCTTAGCT AGAAAAAGTC ACTTGAAAGG GGATGTGATT 
ACTGAGACCG AGTTGAAGAA ATTTTGGGAA CAAATAAATG ATAAAAGCTT TGATTCAAGG 
CTTATCACGT TCTTTGACTT GATGGATAAA GACTCGGATG GTAGACTTAC GGAAGACGAA 
GTTAGAGAGA TCATCAAGCT TAGTTCTTCT GCCAATCATC TGTCATGCAT CCAAAACAAG 
GCAGATGAAT ATGCAGCAAT GATAATGGAA GAGCTTGACC CTGATCATAT GGGATACATC 
ATGGTAAACT TTCTTGATCA TGATATTAAT AGTGAAGAAA GAAAGGAGCT GAGCGACATG 
CTAACTGAGA GTCTCAAGCC TACACGTGAC CCTAACCATT TGAGGAGATG GTATTGTCAA 
CTAAGATTCT TTGTATTGGA TAGTTGGCAA AGAGTTTGGG TGATAGCGTT ATGGCTCACG 
ATTATGGCCA TCCTCTTCGC GTACAAGTAT ATTCAATACA AGAATAGAGC AGTGTATGAA 
GTGTTGGGGC CTTGTGTGTG CTTGGCTAAA GGTGCAGCAG AGACGCTGAA GCTAAACATG 
GCCCTAATTC TGTTACCTGT TTGCAGAAAT ACCATCACAT GGCTTAGAAA TAAGACTAGG 
TTGGGTGTTT TTGTTCCTTT TGATGACAAC CTCAATTTTC ACAAGGTTAT AGCGGTGGGA 
ATTGCGATAG GAGTAGCCAT ACACTCTGTG TCCCACTTGG CGTGTGATTT TCCACTGCTA 
ATCGCGGCAA CTCCAGCGGA ATACATGCCT CTCGGAAAAT TCTTCGGAGA GGAGCAACCA 
AAGAGATACT TGCATTTTGT GAAGTCAACA GAAGGAATAA CAGGGCTTGT AATGGTTTTC 
TTGATGGTTA TTGCTTTTAC ACTGGCTATG CCTTGGTTTA GACGGGGGAA GCTAGAGAAA 
AAGCTACCAG GGCCACTAAA GAAACTTGCG AGCTTCAATG CCTTCTGGTA CACTCACCAT 
TTGTTCGTTA TAGTCTACAT TCTCCTTGTC CTTCACGGAT ACTACATATA TCTCAACAAG 
GAATGGTACA AGAAAACGAC GTGGATGTAC TTGGCAGTAC CAGTAGCTCT TTATGCATAC 
GAGAGATTGA TACGAGCATT CAGGTCCAGC ATCAGGACAG TAAAAGTCCT TAAGATGGCA 
GCTTATCCTG GAAAAGTATT GACTTTGCAG ATGTCAAAGC CTACAAACTT CAAATACATG 
AGCGGCCAAT ACATGTTTGT AAATTGTCCA GCAGTTTCAC CGTTTGAGTG GCATCCATTT 
TCAATAACTT CTACACCACA AGACGACTAT CTGAGCGTTC ATATAAAAGC ATTGGGAGAT 
TGGACAGAGG CTATCCAAGG AGTTTTCTCA GAGGTGTCTA AGCCACCTCC GGTCGGAGAC 
ATGTTGAATG GTGCAAATAG TCCCCGTTTC CCCAAAATTA TGATTGATGG TCCGTACGGC 
GCACCAGCTC AAGACTACAA GAAGTACGAG GTGGTCCTGC TGATAGGTCT TGGCATTGGA 
GCCACCCCGA TGATCAGCAT TATCAAGGAC ATTATTAACA ATACGGAGAC CAAGGAACAA 
CTCAGCCAAA TGGAGAAGGG ATCGCCACAA GAGCAACAAG GTAATAAAGA AACTTTCAAG 
ACTCGAAGGG CTTACTTCTA CTGGGTAACA AAGGAGCAGG GCACATTTGA TTGGTTCAAG 
AACATCATGA ACGAGATCGC AGAACGGGAC AAAAGTAAAG TCATAGAACT ACACAATCAT 
TGCACTAGTG TCTACGAAGA AGGAGACGTT CGTTCCGCGC TCATACGGAT GCTTCAGTCT 
CTTAATTATG CCAAGAATGG TTTAGACATT GTTGCTGGGA CAAGGGTCAT GTCCCATTTC 
GCCCGACCTA ATTGGAAAAA CGTCTACAAA CAGATAGCCA TGGATCACCC TGGCGCTAAT 
GTTGGAGTGT TCTATTGTGG AGCACCGGTA TTGACGAAGG AGTTAAGGCA ATTAGCTTTG 
GAATTCACAC ACAAGACCAG TACTAGATTC TCTTTCCACA AAGAGAACTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001190833.1
CDS	103..2616
Translation	MQRVSFEVKDTEAEKSSSEILSGSLPSTYRNPAMENVGNAVDDGSSVKNNPKLDMQKQNGLVKWFKKCLTMVSGESKAPRLDRSKSTAGQALKGLKIISKTDGNAAWTVVEKRYLKITANTDGLLLRSKFGECIGMNSKEFALELFDALARKSHLKGDVITETELKKFWEQINDKSFDSRLITFFDLMDKDSDGRLTEDEVREIIKLSSSANHLSCIQNKADEYAAMIMEELDPDHMGYIMVNFLDHDINSEERKELSDMLTESLKPTRDPNHLRRWYCQLRFFVLDSWQRVWVIALWLTIMAILFAYKYIQYKNRAVYEVLGPCVCLAKGAAETLKLNMALILLPVCRNTITWLRNKTRLGVFVPFDDNLNFHKVIAVGIAIGVAIHSVSHLACDFPLLIAATPAEYMPLGKFFGEEQPKRYLHFVKSTEGITGLVMVFLMVIAFTLAMPWFRRGKLEKKLPGPLKKLASFNAFWYTHHLFVIVYILLVLHGYYIYLNKEWYKKTTWMYLAVPVALYAYERLIRAFRSSIRTVKVLKMAAYPGKVLTLQMSKPTNFKYMSGQYMFVNCPAVSPFEWHPFSITSTPQDDYLSVHIKALGDWTEAIQGVFSEVSKPPPVGDMLNGANSPRFPKIMIDGPYGAPAQDYKKYEVVLLIGLGIGATPMISIIKDIINNTETKEQLSQMEKGSPQEQQGNKETFKTRRAYFYWVTKEQGTFDWFKNIMNEIAERDKSKVIELHNHCTSVYEEGDVRSALIRMLQSLNYAKNGLDIVAGTRVMSHFARPNWKNVYKQIAMDHPGANVGVFYCGAPVLTKELRQLALEFTHKTSTRFSFHKENF

Target ORF information:

RefSeq Version	NM_001203904.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Riboflavin synthase-like superfamily protein (AT4G25090), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001203904.1

ORF Insert Sequence:

ATGCAGAGAG TGAGCTTTGA AGTAAAGGAC ACAGAGGCGG AGAAGAGCAG CAGCGAGATA 
CTGAGCGGAT CTTTGCCGTC GACCTACCGA AATCCGGCGA TGGAAAACGT TGGAAACGCG 
GTGGATGATG GATCGAGCGT GAAGAACAAT CCGAAACTTG ATATGCAGAA ACAGAACGGT 
TTGGTGAAGT GGTTCAAGAA GTGCTTGACA ATGGTATCTG GAGAGAGTAA GGCACCTCGA 
TTGGACCGGT CGAAGTCAAC GGCCGGTCAG GCCTTGAAAG GGCTCAAGAT CATCAGTAAG 
ACCGACGGTA ACGCTGCCTG GACCGTCGTG GAGAAGCGGT ACTTGAAGAT CACGGCCAAT 
ACTGATGGAT TGCTGCTTCG GTCGAAATTC GGTGAATGCA TAGGGATGAA CTCAAAAGAA 
TTTGCCTTGG AATTGTTTGA CGCCTTAGCT AGAAAAAGTC ACTTGAAAGG GGATGTGATT 
ACTGAGACCG AGTTGAAGAA ATTTTGGGAA CAAATAAATG ATAAAAGCTT TGATTCAAGG 
CTTATCACGT TCTTTGACTT GATGGATAAA GACTCGGATG GTAGACTTAC GGAAGACGAA 
GTTAGAGAGA TCATCAAGCT TAGTTCTTCT GCCAATCATC TGTCATGCAT CCAAAACAAG 
GCAGATGAAT ATGCAGCAAT GATAATGGAA GAGCTTGACC CTGATCATAT GGGATACATC 
ATGGTAAACT TTCTTGATCA TGATATTAAT AGTGAAGAAA GAAAGGAGCT GAGCGACATG 
CTAACTGAGA GTCTCAAGCC TACACGTGAC CCTAACCATT TGAGGAGATG GTATTGTCAA 
CTAAGATTCT TTGTATTGGA TAGTTGGCAA AGAGTTTGGG TGATAGCGTT ATGGCTCACG 
ATTATGGCCA TCCTCTTCGC GTACAAGTAT ATTCAATACA AGAATAGAGC AGTGTATGAA 
GTGTTGGGGC CTTGTGTGTG CTTGGCTAAA GGTGCAGCAG AGACGCTGAA GCTAAACATG 
GCCCTAATTC TGTTACCTGT TTGCAGAAAT ACCATCACAT GGCTTAGAAA TAAGACTAGG 
TTGGGTGTTT TTGTTCCTTT TGATGACAAC CTCAATTTTC ACAAGGTTAT AGCGGTGGGA 
ATTGCGATAG GAGTAGCCAT ACACTCTGTG TCCCACTTGG CGTGTGATTT TCCACTGCTA 
ATCGCGGCAA CTCCAGCGGA ATACATGCCT CTCGGAAAAT TCTTCGGAGA GGAGCAACCA 
AAGAGATACT TGCATTTTGT GAAGTCAACA GAAGGAATAA CAGGGCTTGT AATGGTTTTC 
TTGATGGTTA TTGCTTTTAC ACTGGCTATG CCTTGGTTTA GACGGGGGAA GCTAGAGAAA 
AAGCTACCAG GGCCACTAAA GAAACTTGCG AGCTTCAATG CCTTCTGGTA CACTCACCAT 
TTGTTCGTTA TAGTCTACAT TCTCCTTGTC CTTCACGGAT ACTACATATA TCTCAACAAG 
GAATGGTACA AGAAAACGAC GTGGATGTAC TTGGCAGTAC CAGTAGCTCT TTATGCATAC 
GAGAGATTGA TACGAGCATT CAGGTCCAGC ATCAGGACAG TAAAAGTCCT TAAGATGGCA 
GCTTATCCTG GAAAAGTATT GACTTTGCAG ATGTCAAAGC CTACAAACTT CAAATACATG 
AGCGGCCAAT ACATGTTTGT AAATTGTCCA GCAGTTTCAC CGTTTGAGTG GCATCCATTT 
TCAATAACTT CTACACCACA AGACGACTAT CTGAGCGTTC ATATAAAAGC ATTGGGAGAT 
TGGACAGAGG CTATCCAAGG AGTTTTCTCA GAGGTGTCTA AGCCACCTCC GGTCGGAGAC 
ATGTTGAATG GTGCAAATAG TCCCCGTTTC CCCAAAATTA TGATTGATGG TCCGTACGGC 
GCACCAGCTC AAGACTACAA GAAGTACGAG GTGGTCCTGC TGATAGGTCT TGGCATTGGA 
GCCACCCCGA TGATCAGCAT TATCAAGGAC ATTATTAACA ATACGGAGAC CAAGGAACAA 
CTCAGCCAAA TGGAGAAGGG ATCGCCACAA GAGCAACAAG GTAATAAAGA AACTTTCAAG 
ACTCGAAGGG CTTACTTCTA CTGGGTAACA AAGGAGCAGG GCACATTTGA TTGGTTCAAG 
AACATCATGA ACGAGATCGC AGAACGGGAC AAAAGTAAAG TCATAGAACT ACACAATCAT 
TGCACTAGTG TCTACGAAGA AGGAGACGTT CGTTCCGCGC TCATACGGAT GCTTCAGTCT 
CTTAATTATG CCAAGAATGG TTTAGACATT GTTGCTGGGA CAAGGGTCAT GTCCCATTTC 
GCCCGACCTA ATTGGAAAAA CGTCTACAAA CAGATAGCCA TGGATCACCC TGGCGCTAAT 
GTTGGAGTGT TCTATTGTGG AGCACCGGTA TTGACGAAGG AGTTAAGGCA ATTAGCTTTG 
GAATTCACAC ACAAGACCAG TACTAGATTC TCTTTCCACA AAGAGAACTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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AT4G25090 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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