Duox1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Duox1
Entrez Gene ID	99439
Full Name	dual oxidase 1
Synonyms	9930101G15Rik,AW987690,Duox2,LNOX1,LNOX2,NOXEF1,NOXEF2,P138-TOX,THOX1,THOX2
General protein information	Preferred Names dual oxidase 1 Names dual oxidase 1 dual oxidase 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E5

mRNA and Protein(s)

mRNA	Protein	Name
XM_030252279.1	XP_030108139.1	dual oxidase 1 isoform X1
XM_006500487.3	XP_006500550.1	dual oxidase 1 isoform X1
NM_001099297.1	NP_001092767.1	dual oxidase 1 precursor
XM_006500487.2	XP_006500550.1	dual oxidase 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DUOX1	XP_001163196.1
Arabidopsis thaliana (thale cress)	RBOHD	NP_199602.1
Arabidopsis thaliana (thale cress)	AT4G25090	NP_194239.2
Mus musculus (house mouse)	Duox1	NP_001092767.1
Danio rerio (zebrafish)	duox	XP_001919394.4
Arabidopsis thaliana (thale cress)	RBOHA	NP_196356.1
Oryza sativa (rice)	Os01g0360200	NP_001043020.1
Rattus norvegicus (Norway rat)	Duox1	NP_714961.1
Xenopus tropicalis (tropical clawed frog)	duox1	XP_002937936.2
	DUOX1	XP_001111370.1
Canis lupus familiaris (dog)	DUOX1	NP_001003122.1
Gallus gallus (chicken)	DUOX2	XP_425053.4
Homo sapiens (human)	DUOX1	NP_059130.2
Bos taurus (cattle)	DUOX1	NP_001192603.1
Arabidopsis thaliana (thale cress)	RHD2	NP_199919.1

Gene Ontology
Bibliography

Related articles in PubMed

Autophagy regulates DUOX1 localization and superoxide production in airway epithelial cells during chronic IL-13 stimulation.
Dickinson JD, Sweeter JM, Warren KJ, Ahmad IM, De Deken X, Zimmerman MC, Brody SL
Redox biology14272-284(2018 Apr)

Cigarette Smoke Impairs A
Tian Z, Zhang H, Dixon J, Traphagen N, Wyatt TA, Kharbanda K, Simet Chadwick S, Kolliputi N, Allen-Gipson DS
Scientific reports744405(2017 Mar)

Overexpression of Interleukin-4 in the Thyroid of Transgenic Mice Upregulates the Expression of Duox1 and the Anion Transporter Pendrin.
Eskalli Z, Achouri Y, Hahn S, Many MC, Craps J, Refetoff S, Liao XH, Dumont JE, Van Sande J, Corvilain B, Miot F, De Deken X
Thyroid : official journal of the American Thyroid Association26(10)1499-1512(2016 Oct)

The NADPH Oxidases DUOX1 and NOX2 Play Distinct Roles in Redox Regulation of Epidermal Growth Factor Receptor Signaling.
Heppner DE, Hristova M, Dustin CM, Danyal K, Habibovic A, van der Vliet A
The Journal of biological chemistry291(44)23282-23293(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Redox-dependent regulation of EGFR activation in airway epithelial cells was found to strongly depend on activation of either DUOX1 or NOX2.
Title: The NADPH Oxidases DUOX1 and NOX2 Play Distinct Roles in Redox Regulation of Epidermal Growth Factor Receptor Signaling.

Our findings implicate epithelial DUOX1 as a pivotal mediator of IL-33-dependent activation of innate airway type 2 immune responses to common airborne allergens and indicate that enhanced DUOX1 expression and IL-33 secretion might present important contributing features of allergic asthma.
Title: Airway epithelial dual oxidase 1 mediates allergen-induced IL-33 secretion and activation of type 2 immune responses.

findings suggest that LPS-TLR-4-induced KC or MIP-2 cytokine induction and subsequent neutrophil recruitment in the airway does not require DUOX-derived hydrogen peroxide from airway epithelium
Title: DUOX-Mediated Signaling Is Not Required for LPS-Induced Neutrophilic Response in the Airways.

Studies demonstrate the importance of DUOX1 in epithelial redox signaling through reversible S-glutathionylation of a range of proteins, including proteins involved in cytoskeletal regulation and MAPK signaling pathways involved in cell migration.
Title: Identification of DUOX1-dependent redox signaling through protein S-glutathionylation in airway epithelial cells.

These findings suggest that DUOX-derived H2O2 has an important role in signaling neutrophils into allergic airways
Title: Dual oxidase regulates neutrophil recruitment in allergic airways.

The following Duox1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Duox1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu03260	XM_030252279.1 Latest version!	Mus musculus dual oxidase 1 (Duox1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu03260	XM_006500487.3 Latest version!	Mus musculus dual oxidase 1 (Duox1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu03260	NM_001099297.1 Latest version!	Mus musculus dual oxidase 1 (Duox1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu03260	XM_006500487.2	Mus musculus dual oxidase 1 (Duox1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Duox1 ( XM_030252279.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108139.1 Mus musculus dual oxidase 1 (Duox1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu03260
Clone ID Related Accession (Same CDS sequence)	XM_030252279.1 , XM_006500487.3 , NM_001099297.1 , XM_006500487.2
Accession Version	XM_030252279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	dual oxidase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC ACTTAGCTCT GGCCTGGATT CTCCTGGTTG GGACACTGGC TTCTTTGGGA 
GCTCAGAACT CCATTTCTTG GGAGGTACAG CGATTTGATG GATGGTATAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCTCGG CTGCAGCGCC TTGTTCCAGC CAGCTATGCA 
GATGGTGTCT ACCAGCCCCT GAAAGAACCC TACCTGCCTA ACCCCCGACA CCTTAGTAAC 
AGGGTCATGA GAGGTTCTGC AGGGCAGCCA TCTCTTCGGA ACCGTACAGT CCTGGGTGTC 
TTCTTTGGCT ACCACGTGCT CTCAGACCTG GTGAGTGTGG AAACACCAGG CTGCCCCGCA 
GAGTTCCTCA ACATTTACAT CCCGCATGGG GACCCGGTGT TCGACCCTGA CAAGCGCGGG 
AACGTGGTGC TGCCCTTTCA AAGGAGCCGC TGGGACCGCA ACACTGGACA GAGCCCCAGC 
AACCCCCGGG ACCAGAGCAA CCAGGTGACC GGCTGGCTAG ATGGCAGCGC CATCTATGGC 
TCCTCTCATT CCTGGAGTGA CACTCTGAGG AGCTTCTCTG GAGGACAGCT GGCTTCTGGG 
CCTGACCCTG CTTTCCCATC GGACTCCCAG AGCTCTCTGC TCATGTGGAT GGCGCCGGAC 
CCCTCCACAG GGCAGGGAGG GCCACGGGGG GTGTATGCCT TCGGGGCCCA GCGCGGGAAC 
AGGGAGCCCT TTCTGCAGGC TCTGGGCTTG TTGTGGTTTC GCTACCACAA CCTGTGTGCC 
AGGAAGCTGG CGCAGGAGCA CCCGCACTGG GGGGATGAGG AACTGTTCCA GCATGCTCGC 
AAGAGGGTCA TTGCCACCTA CCAGAACATT GCGATGTATG AGTGGCTGCC CAGCTTCCTG 
AAGCAAACTC CTCCGGAGTA TCCAGGATAC CGTCCATTTC TGGACCCCAG TATCTCCCCA 
GAGTTCGTTG TGGCCTCTGA GCAGTTCCTC TCTACCATGG TGCCCTCTGG GGTCTACATG 
AGGAATGCCA GTTGTCATTT CCAGGGGATT CCCAGTCATA ACTCAAGTGT CTCTGGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGTCGA GAGCACCCCA AGCTACAAAG GGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATTGCTG AGAGAGAGGA CCACGTGGTG 
GTTGAGGACA TGCAAGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGTAC AGACTACCTG 
GCCAGCTGCC TGCAGAGGGG CCGGGATCTA GGCCTGCCTT CTTACACCAA AGCCAGGGAG 
GCACTGGGCC TGTCTCCCAT TTCCCACTGG CAGGACATCA ACCCTGCACT CTCCAGGAGC 
AATGGCACTG TGCTGGAGGC CACAGCTGCC CTGTACAATC AGGACCTGTC TCGGCTGGAG 
CTACTCCCAG GGGGGCTTCT GGAGAGCCAT GGAGACCCTG GACCCCTTTT CAGCACTATT 
GTCCTTGACC AGTTTGTCAG GCTACGAGAT GGTGACCGTT ACTGGTTTGA AAACACCAGG 
AACGGATTGT TCTCTAAGGA AGAGATCGCA GAGATCCGAA ACACTTCCCT ACGGGACATT 
CTAGTGGCTG TCACCAATGT GGACCCCAGT GCCCTACAGC CCAATGTCTT CTTTTGGCTT 
GCAGGAGACC CCTGTCCACA GCCAAGTCAG CTCAGCGCCA AGGGCCTGCC AGCCTGTGCC 
CCCCTCTTCA TTCGGGACTA TTTCGAGGGG AGTGGATTTG GCTTCGGACT CACCATTGGG 
ACCCTTTGCT GTTTCCCCTT AGTCAGCCTC CTTAGTGCCT GGATTGTTGC CCGGCTTCGA 
AAGAGGAATT TCAAGAGGCT CCAGAGACAA GACCGCCAGA GCATCATGTC GGAGAAGCTT 
GTAGGGGGAG TGGAAGCTTT GGAGTGGCAG GGCCGCAATG AACCCTGCAG GCCAGTGCTT 
GTGCACTTAC AGCCGGGACA GATTCGAGTA GTGGATGGCA GGCTCACCGT GCTCCGGACC 
ATCCAGCTGC GACCCCCACA GCAGGTCAAC CTCATCCTGT CCAGCAACCG TGGACGCCGC 
ACCCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTTC TGTTGTTTAA CATGGAGGAG 
GAACGGCAGG CACTGGTGGA AAACGTCAGG GGAGCTCTGA AGGAAAATGG GCTGAGCTTC 
CAGGAGTGGG AACTGAGGGA GCAGGAGCTG ATGAGGGCAG CAGTGACCAG GCAACAACGG 
GGCCACCTCC TGGAGACCTT CTTCAGGCAC CTTTTCTCCC AGGTGTTAGA CATCAATCAG 
GCTGATGCAG GGACTCTGCC TCTGGATTCC TCGACCAAAG TGCGTGAGGC CCTGACCTGT 
GAGCTGAGCA GAGCTGAGTT TGCAGACTCC CTAGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TTTCTCTAGC CGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGGGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCCCCAGAGG AGAAGTCTCG TCTTATGTTC 
CGAATGTATG ACTTTGATGG GAACGGTCTC ATTTCCAAGG ATGAGTTCAT TAGGATGCTA 
AGGTCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTT 
GAGTCTATGT TCCGGGAGTC TGGCTTCCAG GACAAGGAGG AGCTGACCTG GGAGGACTTC 
CACTTCATGC TGCGGGACCA CGACAGTGAT CTCCGATTCA CACAGCTCTG TGTCAAAGGT 
GTGGAAGTGC CGGAAGTGAT CAAGAACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAG 
AAGATCTGCC CTTCGCCCAG AATGAGCGCC CACTGTGCCC GAAACAACAT GAAGACTGCG 
TCATCACCAC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCTCAAGA GATTCGACGG 
AGGTTTGGCA AAAAGGTAAC TTCATTCCAG CCCTTACTGT TCACTGAGGC ACACCGAGAG 
AAGTTTCAAC GCAGCCGCCG CCACCAAACG GTGCAGCAGT TCAAGCGCTT TATTGAGAAT 
TACAGGCGCC ACATTGGCTG TGTGGCGGTG TTTTACACCA TCACTGGGGC GCTCTTCCTG 
GAGAGAGCCT ACTACTATGC ATTTGCAGCA CACCACAGCG GCATCACCGA TACCACCCGT 
GTAGGCATCA TCCTGTCCCG GGGCACGGCG GCCAGCATCT CCTTTATGTT TTCCTACATC 
CTGCTCACCA TGTGTCGCAA CCTCATCACC TTCTTGCGGG AGACCTTCCT CAACCGCTAC 
ATTCCCTTCG ATGCTGCCGT GGACTTCCAT CGCCTCATCG CCTCCACGGC CATCATCCTC 
ACAGTCTTAC ACAGTGCTGG CCACGTAGTG AATGTGTACC TGTTCTCCAT CAGTCCACTC 
AGTGTCCTCT CTTGCCTCTT CCCTGGCCTC TTCCACGATG ATGGGTCGGA GTTCCCTCAG 
AAGTATTACT GGTGGTTCTT CCAGACTGTG CCAGGTCTCA CTGGGGTCCT CCTGCTCCTG 
GCCCTGGCCA TCATGTACGT CTTCGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCATCACCT CTACATTTTT CTCTACATTC TGCTCATCAT ACATGGCAGC 
TTTGCTCTGA TCCAGATGCC CCGGTTCCAC ATCTTCTTCC TGGTCCCTGC AATTATTTAT 
GTGGGGGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCT 
GAGCTGCTGC CATCAGGAGT GACCCACCTG CGGTTCCAGA GGCCTCAGGG CTTTGAGTAT 
AAGTCAGGGC AGTGGGTTCG GATTGCGTGC CTGGCTCTGG GAACCACCGA GTATCACCCT 
TTCACACTGA CTTCTGCACC CCACGAGGAC ACACTCAGCC TGCACATCCG GGCGGCTGGA 
CCCTGGACCA CTCGCCTCAG GGAAATCTAC TCACCCCCAA CGGGTGACAC CTGTGCCAGA 
TACCCAAAGC TGTACCTCGA TGGACCGTTT GGAGAGGGCC ACCAGGAGTG GCACAAGTTT 
GAGGTGTCAG TGCTGGTAGG AGGGGGCATT GGAGTCACTC CCTTTGCCTC CATCCTCAAA 
GACCTGGTTT TCAAGTCATC AGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACAA GGACTCAGAG GCAGTTTGAG TGGCTGGCTG ACATCATCCG GGAGGTGGAG 
GAGAATGACC GCCAGGACCT GGTGTCTGTG CACATCTATA TTACTCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACCAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTGCTGAAC 
AGGAGTCTGT TCACGGGCCT GCGCTCCATC ACCCACTTTG GTCGCCCTCC CTTTGAGCCC 
TTTTTCAACT CTCTGCAGGA AGTCCACCCC CAAGTCCGAA AGATTGGAGT ATTTAGCTGT 
GGTCCCCCTG GCATGACCAA GAATGTGGAG AAGGCTTGTC AGCTCATCAA CAGGCAGGAC 
AGGACTCATT TTTCTCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108139.1
CDS	729..5384
Translation	MGFHLALAWILLVGTLASLGAQNSISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLKEPYLPNPRHLSNRVMRGSAGQPSLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIYIPHGDPVFDPDKRGNVVLPFQRSRWDRNTGQSPSNPRDQSNQVTGWLDGSAIYGSSHSWSDTLRSFSGGQLASGPDPAFPSDSQSSLLMWMAPDPSTGQGGPRGVYAFGAQRGNREPFLQALGLLWFRYHNLCARKLAQEHPHWGDEELFQHARKRVIATYQNIAMYEWLPSFLKQTPPEYPGYRPFLDPSISPEFVVASEQFLSTMVPSGVYMRNASCHFQGIPSHNSSVSGALRVCNSYWSREHPKLQRAEDVDALLLGMASQIAEREDHVVVEDMQDFWPGPLKFSRTDYLASCLQRGRDLGLPSYTKAREALGLSPISHWQDINPALSRSNGTVLEATAALYNQDLSRLELLPGGLLESHGDPGPLFSTIVLDQFVRLRDGDRYWFENTRNGLFSKEEIAEIRNTSLRDILVAVTNVDPSALQPNVFFWLAGDPCPQPSQLSAKGLPACAPLFIRDYFEGSGFGFGLTIGTLCCFPLVSLLSAWIVARLRKRNFKRLQRQDRQSIMSEKLVGGVEALEWQGRNEPCRPVLVHLQPGQIRVVDGRLTVLRTIQLRPPQQVNLILSSNRGRRTLLLKIPKEYDLVLLFNMEEERQALVENVRGALKENGLSFQEWELREQELMRAAVTRQQRGHLLETFFRHLFSQVLDINQADAGTLPLDSSTKVREALTCELSRAEFADSLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHDSDLRFTQLCVKGVEVPEVIKNLCRRASYISQEKICPSPRMSAHCARNNMKTASSPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSRRHQTVQQFKRFIENYRRHIGCVAVFYTITGALFLERAYYYAFAAHHSGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYIPFDAAVDFHRLIASTAIILTVLHSAGHVVNVYLFSISPLSVLSCLFPGLFHDDGSEFPQKYYWWFFQTVPGLTGVLLLLALAIMYVFASHHFRRRSFRGFWLTHHLYIFLYILLIIHGSFALIQMPRFHIFFLVPAIIYVGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSPPTGDTCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDRQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF

Target ORF information:

RefSeq Version	XM_030252279.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dual oxidase 1 (Duox1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252279.1

ORF Insert Sequence:

ATGGGGTTCC ACTTAGCTCT GGCCTGGATT CTCCTGGTTG GGACACTGGC TTCTTTGGGA 
GCTCAGAACT CCATTTCTTG GGAGGTACAG CGATTTGATG GATGGTATAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCTCGG CTGCAGCGCC TTGTTCCAGC CAGCTATGCA 
GATGGTGTCT ACCAGCCCCT GAAAGAACCC TACCTGCCTA ACCCCCGACA CCTTAGTAAC 
AGGGTCATGA GAGGTTCTGC AGGGCAGCCA TCTCTTCGGA ACCGTACAGT CCTGGGTGTC 
TTCTTTGGCT ACCACGTGCT CTCAGACCTG GTGAGTGTGG AAACACCAGG CTGCCCCGCA 
GAGTTCCTCA ACATTTACAT CCCGCATGGG GACCCGGTGT TCGACCCTGA CAAGCGCGGG 
AACGTGGTGC TGCCCTTTCA AAGGAGCCGC TGGGACCGCA ACACTGGACA GAGCCCCAGC 
AACCCCCGGG ACCAGAGCAA CCAGGTGACC GGCTGGCTAG ATGGCAGCGC CATCTATGGC 
TCCTCTCATT CCTGGAGTGA CACTCTGAGG AGCTTCTCTG GAGGACAGCT GGCTTCTGGG 
CCTGACCCTG CTTTCCCATC GGACTCCCAG AGCTCTCTGC TCATGTGGAT GGCGCCGGAC 
CCCTCCACAG GGCAGGGAGG GCCACGGGGG GTGTATGCCT TCGGGGCCCA GCGCGGGAAC 
AGGGAGCCCT TTCTGCAGGC TCTGGGCTTG TTGTGGTTTC GCTACCACAA CCTGTGTGCC 
AGGAAGCTGG CGCAGGAGCA CCCGCACTGG GGGGATGAGG AACTGTTCCA GCATGCTCGC 
AAGAGGGTCA TTGCCACCTA CCAGAACATT GCGATGTATG AGTGGCTGCC CAGCTTCCTG 
AAGCAAACTC CTCCGGAGTA TCCAGGATAC CGTCCATTTC TGGACCCCAG TATCTCCCCA 
GAGTTCGTTG TGGCCTCTGA GCAGTTCCTC TCTACCATGG TGCCCTCTGG GGTCTACATG 
AGGAATGCCA GTTGTCATTT CCAGGGGATT CCCAGTCATA ACTCAAGTGT CTCTGGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGTCGA GAGCACCCCA AGCTACAAAG GGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATTGCTG AGAGAGAGGA CCACGTGGTG 
GTTGAGGACA TGCAAGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGTAC AGACTACCTG 
GCCAGCTGCC TGCAGAGGGG CCGGGATCTA GGCCTGCCTT CTTACACCAA AGCCAGGGAG 
GCACTGGGCC TGTCTCCCAT TTCCCACTGG CAGGACATCA ACCCTGCACT CTCCAGGAGC 
AATGGCACTG TGCTGGAGGC CACAGCTGCC CTGTACAATC AGGACCTGTC TCGGCTGGAG 
CTACTCCCAG GGGGGCTTCT GGAGAGCCAT GGAGACCCTG GACCCCTTTT CAGCACTATT 
GTCCTTGACC AGTTTGTCAG GCTACGAGAT GGTGACCGTT ACTGGTTTGA AAACACCAGG 
AACGGATTGT TCTCTAAGGA AGAGATCGCA GAGATCCGAA ACACTTCCCT ACGGGACATT 
CTAGTGGCTG TCACCAATGT GGACCCCAGT GCCCTACAGC CCAATGTCTT CTTTTGGCTT 
GCAGGAGACC CCTGTCCACA GCCAAGTCAG CTCAGCGCCA AGGGCCTGCC AGCCTGTGCC 
CCCCTCTTCA TTCGGGACTA TTTCGAGGGG AGTGGATTTG GCTTCGGACT CACCATTGGG 
ACCCTTTGCT GTTTCCCCTT AGTCAGCCTC CTTAGTGCCT GGATTGTTGC CCGGCTTCGA 
AAGAGGAATT TCAAGAGGCT CCAGAGACAA GACCGCCAGA GCATCATGTC GGAGAAGCTT 
GTAGGGGGAG TGGAAGCTTT GGAGTGGCAG GGCCGCAATG AACCCTGCAG GCCAGTGCTT 
GTGCACTTAC AGCCGGGACA GATTCGAGTA GTGGATGGCA GGCTCACCGT GCTCCGGACC 
ATCCAGCTGC GACCCCCACA GCAGGTCAAC CTCATCCTGT CCAGCAACCG TGGACGCCGC 
ACCCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTTC TGTTGTTTAA CATGGAGGAG 
GAACGGCAGG CACTGGTGGA AAACGTCAGG GGAGCTCTGA AGGAAAATGG GCTGAGCTTC 
CAGGAGTGGG AACTGAGGGA GCAGGAGCTG ATGAGGGCAG CAGTGACCAG GCAACAACGG 
GGCCACCTCC TGGAGACCTT CTTCAGGCAC CTTTTCTCCC AGGTGTTAGA CATCAATCAG 
GCTGATGCAG GGACTCTGCC TCTGGATTCC TCGACCAAAG TGCGTGAGGC CCTGACCTGT 
GAGCTGAGCA GAGCTGAGTT TGCAGACTCC CTAGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TTTCTCTAGC CGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGGGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCCCCAGAGG AGAAGTCTCG TCTTATGTTC 
CGAATGTATG ACTTTGATGG GAACGGTCTC ATTTCCAAGG ATGAGTTCAT TAGGATGCTA 
AGGTCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTT 
GAGTCTATGT TCCGGGAGTC TGGCTTCCAG GACAAGGAGG AGCTGACCTG GGAGGACTTC 
CACTTCATGC TGCGGGACCA CGACAGTGAT CTCCGATTCA CACAGCTCTG TGTCAAAGGT 
GTGGAAGTGC CGGAAGTGAT CAAGAACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAG 
AAGATCTGCC CTTCGCCCAG AATGAGCGCC CACTGTGCCC GAAACAACAT GAAGACTGCG 
TCATCACCAC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCTCAAGA GATTCGACGG 
AGGTTTGGCA AAAAGGTAAC TTCATTCCAG CCCTTACTGT TCACTGAGGC ACACCGAGAG 
AAGTTTCAAC GCAGCCGCCG CCACCAAACG GTGCAGCAGT TCAAGCGCTT TATTGAGAAT 
TACAGGCGCC ACATTGGCTG TGTGGCGGTG TTTTACACCA TCACTGGGGC GCTCTTCCTG 
GAGAGAGCCT ACTACTATGC ATTTGCAGCA CACCACAGCG GCATCACCGA TACCACCCGT 
GTAGGCATCA TCCTGTCCCG GGGCACGGCG GCCAGCATCT CCTTTATGTT TTCCTACATC 
CTGCTCACCA TGTGTCGCAA CCTCATCACC TTCTTGCGGG AGACCTTCCT CAACCGCTAC 
ATTCCCTTCG ATGCTGCCGT GGACTTCCAT CGCCTCATCG CCTCCACGGC CATCATCCTC 
ACAGTCTTAC ACAGTGCTGG CCACGTAGTG AATGTGTACC TGTTCTCCAT CAGTCCACTC 
AGTGTCCTCT CTTGCCTCTT CCCTGGCCTC TTCCACGATG ATGGGTCGGA GTTCCCTCAG 
AAGTATTACT GGTGGTTCTT CCAGACTGTG CCAGGTCTCA CTGGGGTCCT CCTGCTCCTG 
GCCCTGGCCA TCATGTACGT CTTCGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCATCACCT CTACATTTTT CTCTACATTC TGCTCATCAT ACATGGCAGC 
TTTGCTCTGA TCCAGATGCC CCGGTTCCAC ATCTTCTTCC TGGTCCCTGC AATTATTTAT 
GTGGGGGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCT 
GAGCTGCTGC CATCAGGAGT GACCCACCTG CGGTTCCAGA GGCCTCAGGG CTTTGAGTAT 
AAGTCAGGGC AGTGGGTTCG GATTGCGTGC CTGGCTCTGG GAACCACCGA GTATCACCCT 
TTCACACTGA CTTCTGCACC CCACGAGGAC ACACTCAGCC TGCACATCCG GGCGGCTGGA 
CCCTGGACCA CTCGCCTCAG GGAAATCTAC TCACCCCCAA CGGGTGACAC CTGTGCCAGA 
TACCCAAAGC TGTACCTCGA TGGACCGTTT GGAGAGGGCC ACCAGGAGTG GCACAAGTTT 
GAGGTGTCAG TGCTGGTAGG AGGGGGCATT GGAGTCACTC CCTTTGCCTC CATCCTCAAA 
GACCTGGTTT TCAAGTCATC AGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACAA GGACTCAGAG GCAGTTTGAG TGGCTGGCTG ACATCATCCG GGAGGTGGAG 
GAGAATGACC GCCAGGACCT GGTGTCTGTG CACATCTATA TTACTCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACCAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTGCTGAAC 
AGGAGTCTGT TCACGGGCCT GCGCTCCATC ACCCACTTTG GTCGCCCTCC CTTTGAGCCC 
TTTTTCAACT CTCTGCAGGA AGTCCACCCC CAAGTCCGAA AGATTGGAGT ATTTAGCTGT 
GGTCCCCCTG GCATGACCAA GAATGTGGAG AAGGCTTGTC AGCTCATCAA CAGGCAGGAC 
AGGACTCATT TTTCTCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Duox1 ( XM_006500487.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500550.1 Mus musculus dual oxidase 1 (Duox1), transcript variant X1, mRNA.

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CloneID	OMu03260
Clone ID Related Accession (Same CDS sequence)	XM_030252279.1 , XM_006500487.3 , NM_001099297.1 , XM_006500487.2
Accession Version	XM_006500487.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	dual oxidase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500487.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC ACTTAGCTCT GGCCTGGATT CTCCTGGTTG GGACACTGGC TTCTTTGGGA 
GCTCAGAACT CCATTTCTTG GGAGGTACAG CGATTTGATG GATGGTATAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCTCGG CTGCAGCGCC TTGTTCCAGC CAGCTATGCA 
GATGGTGTCT ACCAGCCCCT GAAAGAACCC TACCTGCCTA ACCCCCGACA CCTTAGTAAC 
AGGGTCATGA GAGGTTCTGC AGGGCAGCCA TCTCTTCGGA ACCGTACAGT CCTGGGTGTC 
TTCTTTGGCT ACCACGTGCT CTCAGACCTG GTGAGTGTGG AAACACCAGG CTGCCCCGCA 
GAGTTCCTCA ACATTTACAT CCCGCATGGG GACCCGGTGT TCGACCCTGA CAAGCGCGGG 
AACGTGGTGC TGCCCTTTCA AAGGAGCCGC TGGGACCGCA ACACTGGACA GAGCCCCAGC 
AACCCCCGGG ACCAGAGCAA CCAGGTGACC GGCTGGCTAG ATGGCAGCGC CATCTATGGC 
TCCTCTCATT CCTGGAGTGA CACTCTGAGG AGCTTCTCTG GAGGACAGCT GGCTTCTGGG 
CCTGACCCTG CTTTCCCATC GGACTCCCAG AGCTCTCTGC TCATGTGGAT GGCGCCGGAC 
CCCTCCACAG GGCAGGGAGG GCCACGGGGG GTGTATGCCT TCGGGGCCCA GCGCGGGAAC 
AGGGAGCCCT TTCTGCAGGC TCTGGGCTTG TTGTGGTTTC GCTACCACAA CCTGTGTGCC 
AGGAAGCTGG CGCAGGAGCA CCCGCACTGG GGGGATGAGG AACTGTTCCA GCATGCTCGC 
AAGAGGGTCA TTGCCACCTA CCAGAACATT GCGATGTATG AGTGGCTGCC CAGCTTCCTG 
AAGCAAACTC CTCCGGAGTA TCCAGGATAC CGTCCATTTC TGGACCCCAG TATCTCCCCA 
GAGTTCGTTG TGGCCTCTGA GCAGTTCCTC TCTACCATGG TGCCCTCTGG GGTCTACATG 
AGGAATGCCA GTTGTCATTT CCAGGGGATT CCCAGTCATA ACTCAAGTGT CTCTGGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGTCGA GAGCACCCCA AGCTACAAAG GGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATTGCTG AGAGAGAGGA CCACGTGGTG 
GTTGAGGACA TGCAAGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGTAC AGACTACCTG 
GCCAGCTGCC TGCAGAGGGG CCGGGATCTA GGCCTGCCTT CTTACACCAA AGCCAGGGAG 
GCACTGGGCC TGTCTCCCAT TTCCCACTGG CAGGACATCA ACCCTGCACT CTCCAGGAGC 
AATGGCACTG TGCTGGAGGC CACAGCTGCC CTGTACAATC AGGACCTGTC TCGGCTGGAG 
CTACTCCCAG GGGGGCTTCT GGAGAGCCAT GGAGACCCTG GACCCCTTTT CAGCACTATT 
GTCCTTGACC AGTTTGTCAG GCTACGAGAT GGTGACCGTT ACTGGTTTGA AAACACCAGG 
AACGGATTGT TCTCTAAGGA AGAGATCGCA GAGATCCGAA ACACTTCCCT ACGGGACATT 
CTAGTGGCTG TCACCAATGT GGACCCCAGT GCCCTACAGC CCAATGTCTT CTTTTGGCTT 
GCAGGAGACC CCTGTCCACA GCCAAGTCAG CTCAGCGCCA AGGGCCTGCC AGCCTGTGCC 
CCCCTCTTCA TTCGGGACTA TTTCGAGGGG AGTGGATTTG GCTTCGGACT CACCATTGGG 
ACCCTTTGCT GTTTCCCCTT AGTCAGCCTC CTTAGTGCCT GGATTGTTGC CCGGCTTCGA 
AAGAGGAATT TCAAGAGGCT CCAGAGACAA GACCGCCAGA GCATCATGTC GGAGAAGCTT 
GTAGGGGGAG TGGAAGCTTT GGAGTGGCAG GGCCGCAATG AACCCTGCAG GCCAGTGCTT 
GTGCACTTAC AGCCGGGACA GATTCGAGTA GTGGATGGCA GGCTCACCGT GCTCCGGACC 
ATCCAGCTGC GACCCCCACA GCAGGTCAAC CTCATCCTGT CCAGCAACCG TGGACGCCGC 
ACCCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTTC TGTTGTTTAA CATGGAGGAG 
GAACGGCAGG CACTGGTGGA AAACGTCAGG GGAGCTCTGA AGGAAAATGG GCTGAGCTTC 
CAGGAGTGGG AACTGAGGGA GCAGGAGCTG ATGAGGGCAG CAGTGACCAG GCAACAACGG 
GGCCACCTCC TGGAGACCTT CTTCAGGCAC CTTTTCTCCC AGGTGTTAGA CATCAATCAG 
GCTGATGCAG GGACTCTGCC TCTGGATTCC TCGACCAAAG TGCGTGAGGC CCTGACCTGT 
GAGCTGAGCA GAGCTGAGTT TGCAGACTCC CTAGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TTTCTCTAGC CGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGGGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCCCCAGAGG AGAAGTCTCG TCTTATGTTC 
CGAATGTATG ACTTTGATGG GAACGGTCTC ATTTCCAAGG ATGAGTTCAT TAGGATGCTA 
AGGTCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTT 
GAGTCTATGT TCCGGGAGTC TGGCTTCCAG GACAAGGAGG AGCTGACCTG GGAGGACTTC 
CACTTCATGC TGCGGGACCA CGACAGTGAT CTCCGATTCA CACAGCTCTG TGTCAAAGGT 
GTGGAAGTGC CGGAAGTGAT CAAGAACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAG 
AAGATCTGCC CTTCGCCCAG AATGAGCGCC CACTGTGCCC GAAACAACAT GAAGACTGCG 
TCATCACCAC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCTCAAGA GATTCGACGG 
AGGTTTGGCA AAAAGGTAAC TTCATTCCAG CCCTTACTGT TCACTGAGGC ACACCGAGAG 
AAGTTTCAAC GCAGCCGCCG CCACCAAACG GTGCAGCAGT TCAAGCGCTT TATTGAGAAT 
TACAGGCGCC ACATTGGCTG TGTGGCGGTG TTTTACACCA TCACTGGGGC GCTCTTCCTG 
GAGAGAGCCT ACTACTATGC ATTTGCAGCA CACCACAGCG GCATCACCGA TACCACCCGT 
GTAGGCATCA TCCTGTCCCG GGGCACGGCG GCCAGCATCT CCTTTATGTT TTCCTACATC 
CTGCTCACCA TGTGTCGCAA CCTCATCACC TTCTTGCGGG AGACCTTCCT CAACCGCTAC 
ATTCCCTTCG ATGCTGCCGT GGACTTCCAT CGCCTCATCG CCTCCACGGC CATCATCCTC 
ACAGTCTTAC ACAGTGCTGG CCACGTAGTG AATGTGTACC TGTTCTCCAT CAGTCCACTC 
AGTGTCCTCT CTTGCCTCTT CCCTGGCCTC TTCCACGATG ATGGGTCGGA GTTCCCTCAG 
AAGTATTACT GGTGGTTCTT CCAGACTGTG CCAGGTCTCA CTGGGGTCCT CCTGCTCCTG 
GCCCTGGCCA TCATGTACGT CTTCGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCATCACCT CTACATTTTT CTCTACATTC TGCTCATCAT ACATGGCAGC 
TTTGCTCTGA TCCAGATGCC CCGGTTCCAC ATCTTCTTCC TGGTCCCTGC AATTATTTAT 
GTGGGGGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCT 
GAGCTGCTGC CATCAGGAGT GACCCACCTG CGGTTCCAGA GGCCTCAGGG CTTTGAGTAT 
AAGTCAGGGC AGTGGGTTCG GATTGCGTGC CTGGCTCTGG GAACCACCGA GTATCACCCT 
TTCACACTGA CTTCTGCACC CCACGAGGAC ACACTCAGCC TGCACATCCG GGCGGCTGGA 
CCCTGGACCA CTCGCCTCAG GGAAATCTAC TCACCCCCAA CGGGTGACAC CTGTGCCAGA 
TACCCAAAGC TGTACCTCGA TGGACCGTTT GGAGAGGGCC ACCAGGAGTG GCACAAGTTT 
GAGGTGTCAG TGCTGGTAGG AGGGGGCATT GGAGTCACTC CCTTTGCCTC CATCCTCAAA 
GACCTGGTTT TCAAGTCATC AGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACAA GGACTCAGAG GCAGTTTGAG TGGCTGGCTG ACATCATCCG GGAGGTGGAG 
GAGAATGACC GCCAGGACCT GGTGTCTGTG CACATCTATA TTACTCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACCAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTGCTGAAC 
AGGAGTCTGT TCACGGGCCT GCGCTCCATC ACCCACTTTG GTCGCCCTCC CTTTGAGCCC 
TTTTTCAACT CTCTGCAGGA AGTCCACCCC CAAGTCCGAA AGATTGGAGT ATTTAGCTGT 
GGTCCCCCTG GCATGACCAA GAATGTGGAG AAGGCTTGTC AGCTCATCAA CAGGCAGGAC 
AGGACTCATT TTTCTCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500550.1
CDS	824..5479
Translation	MGFHLALAWILLVGTLASLGAQNSISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLKEPYLPNPRHLSNRVMRGSAGQPSLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIYIPHGDPVFDPDKRGNVVLPFQRSRWDRNTGQSPSNPRDQSNQVTGWLDGSAIYGSSHSWSDTLRSFSGGQLASGPDPAFPSDSQSSLLMWMAPDPSTGQGGPRGVYAFGAQRGNREPFLQALGLLWFRYHNLCARKLAQEHPHWGDEELFQHARKRVIATYQNIAMYEWLPSFLKQTPPEYPGYRPFLDPSISPEFVVASEQFLSTMVPSGVYMRNASCHFQGIPSHNSSVSGALRVCNSYWSREHPKLQRAEDVDALLLGMASQIAEREDHVVVEDMQDFWPGPLKFSRTDYLASCLQRGRDLGLPSYTKAREALGLSPISHWQDINPALSRSNGTVLEATAALYNQDLSRLELLPGGLLESHGDPGPLFSTIVLDQFVRLRDGDRYWFENTRNGLFSKEEIAEIRNTSLRDILVAVTNVDPSALQPNVFFWLAGDPCPQPSQLSAKGLPACAPLFIRDYFEGSGFGFGLTIGTLCCFPLVSLLSAWIVARLRKRNFKRLQRQDRQSIMSEKLVGGVEALEWQGRNEPCRPVLVHLQPGQIRVVDGRLTVLRTIQLRPPQQVNLILSSNRGRRTLLLKIPKEYDLVLLFNMEEERQALVENVRGALKENGLSFQEWELREQELMRAAVTRQQRGHLLETFFRHLFSQVLDINQADAGTLPLDSSTKVREALTCELSRAEFADSLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHDSDLRFTQLCVKGVEVPEVIKNLCRRASYISQEKICPSPRMSAHCARNNMKTASSPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSRRHQTVQQFKRFIENYRRHIGCVAVFYTITGALFLERAYYYAFAAHHSGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYIPFDAAVDFHRLIASTAIILTVLHSAGHVVNVYLFSISPLSVLSCLFPGLFHDDGSEFPQKYYWWFFQTVPGLTGVLLLLALAIMYVFASHHFRRRSFRGFWLTHHLYIFLYILLIIHGSFALIQMPRFHIFFLVPAIIYVGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSPPTGDTCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDRQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF

Target ORF information:

RefSeq Version	XM_006500487.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dual oxidase 1 (Duox1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500487.3

ORF Insert Sequence:

ATGGGGTTCC ACTTAGCTCT GGCCTGGATT CTCCTGGTTG GGACACTGGC TTCTTTGGGA 
GCTCAGAACT CCATTTCTTG GGAGGTACAG CGATTTGATG GATGGTATAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCTCGG CTGCAGCGCC TTGTTCCAGC CAGCTATGCA 
GATGGTGTCT ACCAGCCCCT GAAAGAACCC TACCTGCCTA ACCCCCGACA CCTTAGTAAC 
AGGGTCATGA GAGGTTCTGC AGGGCAGCCA TCTCTTCGGA ACCGTACAGT CCTGGGTGTC 
TTCTTTGGCT ACCACGTGCT CTCAGACCTG GTGAGTGTGG AAACACCAGG CTGCCCCGCA 
GAGTTCCTCA ACATTTACAT CCCGCATGGG GACCCGGTGT TCGACCCTGA CAAGCGCGGG 
AACGTGGTGC TGCCCTTTCA AAGGAGCCGC TGGGACCGCA ACACTGGACA GAGCCCCAGC 
AACCCCCGGG ACCAGAGCAA CCAGGTGACC GGCTGGCTAG ATGGCAGCGC CATCTATGGC 
TCCTCTCATT CCTGGAGTGA CACTCTGAGG AGCTTCTCTG GAGGACAGCT GGCTTCTGGG 
CCTGACCCTG CTTTCCCATC GGACTCCCAG AGCTCTCTGC TCATGTGGAT GGCGCCGGAC 
CCCTCCACAG GGCAGGGAGG GCCACGGGGG GTGTATGCCT TCGGGGCCCA GCGCGGGAAC 
AGGGAGCCCT TTCTGCAGGC TCTGGGCTTG TTGTGGTTTC GCTACCACAA CCTGTGTGCC 
AGGAAGCTGG CGCAGGAGCA CCCGCACTGG GGGGATGAGG AACTGTTCCA GCATGCTCGC 
AAGAGGGTCA TTGCCACCTA CCAGAACATT GCGATGTATG AGTGGCTGCC CAGCTTCCTG 
AAGCAAACTC CTCCGGAGTA TCCAGGATAC CGTCCATTTC TGGACCCCAG TATCTCCCCA 
GAGTTCGTTG TGGCCTCTGA GCAGTTCCTC TCTACCATGG TGCCCTCTGG GGTCTACATG 
AGGAATGCCA GTTGTCATTT CCAGGGGATT CCCAGTCATA ACTCAAGTGT CTCTGGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGTCGA GAGCACCCCA AGCTACAAAG GGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATTGCTG AGAGAGAGGA CCACGTGGTG 
GTTGAGGACA TGCAAGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGTAC AGACTACCTG 
GCCAGCTGCC TGCAGAGGGG CCGGGATCTA GGCCTGCCTT CTTACACCAA AGCCAGGGAG 
GCACTGGGCC TGTCTCCCAT TTCCCACTGG CAGGACATCA ACCCTGCACT CTCCAGGAGC 
AATGGCACTG TGCTGGAGGC CACAGCTGCC CTGTACAATC AGGACCTGTC TCGGCTGGAG 
CTACTCCCAG GGGGGCTTCT GGAGAGCCAT GGAGACCCTG GACCCCTTTT CAGCACTATT 
GTCCTTGACC AGTTTGTCAG GCTACGAGAT GGTGACCGTT ACTGGTTTGA AAACACCAGG 
AACGGATTGT TCTCTAAGGA AGAGATCGCA GAGATCCGAA ACACTTCCCT ACGGGACATT 
CTAGTGGCTG TCACCAATGT GGACCCCAGT GCCCTACAGC CCAATGTCTT CTTTTGGCTT 
GCAGGAGACC CCTGTCCACA GCCAAGTCAG CTCAGCGCCA AGGGCCTGCC AGCCTGTGCC 
CCCCTCTTCA TTCGGGACTA TTTCGAGGGG AGTGGATTTG GCTTCGGACT CACCATTGGG 
ACCCTTTGCT GTTTCCCCTT AGTCAGCCTC CTTAGTGCCT GGATTGTTGC CCGGCTTCGA 
AAGAGGAATT TCAAGAGGCT CCAGAGACAA GACCGCCAGA GCATCATGTC GGAGAAGCTT 
GTAGGGGGAG TGGAAGCTTT GGAGTGGCAG GGCCGCAATG AACCCTGCAG GCCAGTGCTT 
GTGCACTTAC AGCCGGGACA GATTCGAGTA GTGGATGGCA GGCTCACCGT GCTCCGGACC 
ATCCAGCTGC GACCCCCACA GCAGGTCAAC CTCATCCTGT CCAGCAACCG TGGACGCCGC 
ACCCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTTC TGTTGTTTAA CATGGAGGAG 
GAACGGCAGG CACTGGTGGA AAACGTCAGG GGAGCTCTGA AGGAAAATGG GCTGAGCTTC 
CAGGAGTGGG AACTGAGGGA GCAGGAGCTG ATGAGGGCAG CAGTGACCAG GCAACAACGG 
GGCCACCTCC TGGAGACCTT CTTCAGGCAC CTTTTCTCCC AGGTGTTAGA CATCAATCAG 
GCTGATGCAG GGACTCTGCC TCTGGATTCC TCGACCAAAG TGCGTGAGGC CCTGACCTGT 
GAGCTGAGCA GAGCTGAGTT TGCAGACTCC CTAGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TTTCTCTAGC CGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGGGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCCCCAGAGG AGAAGTCTCG TCTTATGTTC 
CGAATGTATG ACTTTGATGG GAACGGTCTC ATTTCCAAGG ATGAGTTCAT TAGGATGCTA 
AGGTCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTT 
GAGTCTATGT TCCGGGAGTC TGGCTTCCAG GACAAGGAGG AGCTGACCTG GGAGGACTTC 
CACTTCATGC TGCGGGACCA CGACAGTGAT CTCCGATTCA CACAGCTCTG TGTCAAAGGT 
GTGGAAGTGC CGGAAGTGAT CAAGAACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAG 
AAGATCTGCC CTTCGCCCAG AATGAGCGCC CACTGTGCCC GAAACAACAT GAAGACTGCG 
TCATCACCAC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCTCAAGA GATTCGACGG 
AGGTTTGGCA AAAAGGTAAC TTCATTCCAG CCCTTACTGT TCACTGAGGC ACACCGAGAG 
AAGTTTCAAC GCAGCCGCCG CCACCAAACG GTGCAGCAGT TCAAGCGCTT TATTGAGAAT 
TACAGGCGCC ACATTGGCTG TGTGGCGGTG TTTTACACCA TCACTGGGGC GCTCTTCCTG 
GAGAGAGCCT ACTACTATGC ATTTGCAGCA CACCACAGCG GCATCACCGA TACCACCCGT 
GTAGGCATCA TCCTGTCCCG GGGCACGGCG GCCAGCATCT CCTTTATGTT TTCCTACATC 
CTGCTCACCA TGTGTCGCAA CCTCATCACC TTCTTGCGGG AGACCTTCCT CAACCGCTAC 
ATTCCCTTCG ATGCTGCCGT GGACTTCCAT CGCCTCATCG CCTCCACGGC CATCATCCTC 
ACAGTCTTAC ACAGTGCTGG CCACGTAGTG AATGTGTACC TGTTCTCCAT CAGTCCACTC 
AGTGTCCTCT CTTGCCTCTT CCCTGGCCTC TTCCACGATG ATGGGTCGGA GTTCCCTCAG 
AAGTATTACT GGTGGTTCTT CCAGACTGTG CCAGGTCTCA CTGGGGTCCT CCTGCTCCTG 
GCCCTGGCCA TCATGTACGT CTTCGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCATCACCT CTACATTTTT CTCTACATTC TGCTCATCAT ACATGGCAGC 
TTTGCTCTGA TCCAGATGCC CCGGTTCCAC ATCTTCTTCC TGGTCCCTGC AATTATTTAT 
GTGGGGGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCT 
GAGCTGCTGC CATCAGGAGT GACCCACCTG CGGTTCCAGA GGCCTCAGGG CTTTGAGTAT 
AAGTCAGGGC AGTGGGTTCG GATTGCGTGC CTGGCTCTGG GAACCACCGA GTATCACCCT 
TTCACACTGA CTTCTGCACC CCACGAGGAC ACACTCAGCC TGCACATCCG GGCGGCTGGA 
CCCTGGACCA CTCGCCTCAG GGAAATCTAC TCACCCCCAA CGGGTGACAC CTGTGCCAGA 
TACCCAAAGC TGTACCTCGA TGGACCGTTT GGAGAGGGCC ACCAGGAGTG GCACAAGTTT 
GAGGTGTCAG TGCTGGTAGG AGGGGGCATT GGAGTCACTC CCTTTGCCTC CATCCTCAAA 
GACCTGGTTT TCAAGTCATC AGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACAA GGACTCAGAG GCAGTTTGAG TGGCTGGCTG ACATCATCCG GGAGGTGGAG 
GAGAATGACC GCCAGGACCT GGTGTCTGTG CACATCTATA TTACTCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACCAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTGCTGAAC 
AGGAGTCTGT TCACGGGCCT GCGCTCCATC ACCCACTTTG GTCGCCCTCC CTTTGAGCCC 
TTTTTCAACT CTCTGCAGGA AGTCCACCCC CAAGTCCGAA AGATTGGAGT ATTTAGCTGT 
GGTCCCCCTG GCATGACCAA GAATGTGGAG AAGGCTTGTC AGCTCATCAA CAGGCAGGAC 
AGGACTCATT TTTCTCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Duox1 ( NM_001099297.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001092767.1 Mus musculus dual oxidase 1 (Duox1), mRNA.

Price & Availability↑

CloneID	OMu03260
Clone ID Related Accession (Same CDS sequence)	XM_030252279.1 , XM_006500487.3 , NM_001099297.1 , XM_006500487.2
Accession Version	NM_001099297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Mus musculus(house mouse)
Product	dual oxidase 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL844566.8. On or before Jul 25, 2007 this sequence version replaced XM_001480692.1, XM_130483.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGGGGTTCC ACTTAGCTCT GGCCTGGATT CTCCTGGTTG GGACACTGGC TTCTTTGGGA 
GCTCAGAACT CCATTTCTTG GGAGGTACAG CGATTTGATG GATGGTATAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCTCGG CTGCAGCGCC TTGTTCCAGC CAGCTATGCA 
GATGGTGTCT ACCAGCCCCT GAAAGAACCC TACCTGCCTA ACCCCCGACA CCTTAGTAAC 
AGGGTCATGA GAGGTTCTGC AGGGCAGCCA TCTCTTCGGA ACCGTACAGT CCTGGGTGTC 
TTCTTTGGCT ACCACGTGCT CTCAGACCTG GTGAGTGTGG AAACACCAGG CTGCCCCGCA 
GAGTTCCTCA ACATTTACAT CCCGCATGGG GACCCGGTGT TCGACCCTGA CAAGCGCGGG 
AACGTGGTGC TGCCCTTTCA AAGGAGCCGC TGGGACCGCA ACACTGGACA GAGCCCCAGC 
AACCCCCGGG ACCAGAGCAA CCAGGTGACC GGCTGGCTAG ATGGCAGCGC CATCTATGGC 
TCCTCTCATT CCTGGAGTGA CACTCTGAGG AGCTTCTCTG GAGGACAGCT GGCTTCTGGG 
CCTGACCCTG CTTTCCCATC GGACTCCCAG AGCTCTCTGC TCATGTGGAT GGCGCCGGAC 
CCCTCCACAG GGCAGGGAGG GCCACGGGGG GTGTATGCCT TCGGGGCCCA GCGCGGGAAC 
AGGGAGCCCT TTCTGCAGGC TCTGGGCTTG TTGTGGTTTC GCTACCACAA CCTGTGTGCC 
AGGAAGCTGG CGCAGGAGCA CCCGCACTGG GGGGATGAGG AACTGTTCCA GCATGCTCGC 
AAGAGGGTCA TTGCCACCTA CCAGAACATT GCGATGTATG AGTGGCTGCC CAGCTTCCTG 
AAGCAAACTC CTCCGGAGTA TCCAGGATAC CGTCCATTTC TGGACCCCAG TATCTCCCCA 
GAGTTCGTTG TGGCCTCTGA GCAGTTCCTC TCTACCATGG TGCCCTCTGG GGTCTACATG 
AGGAATGCCA GTTGTCATTT CCAGGGGATT CCCAGTCATA ACTCAAGTGT CTCTGGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGTCGA GAGCACCCCA AGCTACAAAG GGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATTGCTG AGAGAGAGGA CCACGTGGTG 
GTTGAGGACA TGCAAGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGTAC AGACTACCTG 
GCCAGCTGCC TGCAGAGGGG CCGGGATCTA GGCCTGCCTT CTTACACCAA AGCCAGGGAG 
GCACTGGGCC TGTCTCCCAT TTCCCACTGG CAGGACATCA ACCCTGCACT CTCCAGGAGC 
AATGGCACTG TGCTGGAGGC CACAGCTGCC CTGTACAATC AGGACCTGTC TCGGCTGGAG 
CTACTCCCAG GGGGGCTTCT GGAGAGCCAT GGAGACCCTG GACCCCTTTT CAGCACTATT 
GTCCTTGACC AGTTTGTCAG GCTACGAGAT GGTGACCGTT ACTGGTTTGA AAACACCAGG 
AACGGATTGT TCTCTAAGGA AGAGATCGCA GAGATCCGAA ACACTTCCCT ACGGGACATT 
CTAGTGGCTG TCACCAATGT GGACCCCAGT GCCCTACAGC CCAATGTCTT CTTTTGGCTT 
GCAGGAGACC CCTGTCCACA GCCAAGTCAG CTCAGCGCCA AGGGCCTGCC AGCCTGTGCC 
CCCCTCTTCA TTCGGGACTA TTTCGAGGGG AGTGGATTTG GCTTCGGACT CACCATTGGG 
ACCCTTTGCT GTTTCCCCTT AGTCAGCCTC CTTAGTGCCT GGATTGTTGC CCGGCTTCGA 
AAGAGGAATT TCAAGAGGCT CCAGAGACAA GACCGCCAGA GCATCATGTC GGAGAAGCTT 
GTAGGGGGAG TGGAAGCTTT GGAGTGGCAG GGCCGCAATG AACCCTGCAG GCCAGTGCTT 
GTGCACTTAC AGCCGGGACA GATTCGAGTA GTGGATGGCA GGCTCACCGT GCTCCGGACC 
ATCCAGCTGC GACCCCCACA GCAGGTCAAC CTCATCCTGT CCAGCAACCG TGGACGCCGC 
ACCCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTTC TGTTGTTTAA CATGGAGGAG 
GAACGGCAGG CACTGGTGGA AAACGTCAGG GGAGCTCTGA AGGAAAATGG GCTGAGCTTC 
CAGGAGTGGG AACTGAGGGA GCAGGAGCTG ATGAGGGCAG CAGTGACCAG GCAACAACGG 
GGCCACCTCC TGGAGACCTT CTTCAGGCAC CTTTTCTCCC AGGTGTTAGA CATCAATCAG 
GCTGATGCAG GGACTCTGCC TCTGGATTCC TCGACCAAAG TGCGTGAGGC CCTGACCTGT 
GAGCTGAGCA GAGCTGAGTT TGCAGACTCC CTAGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TTTCTCTAGC CGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGGGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCCCCAGAGG AGAAGTCTCG TCTTATGTTC 
CGAATGTATG ACTTTGATGG GAACGGTCTC ATTTCCAAGG ATGAGTTCAT TAGGATGCTA 
AGGTCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTT 
GAGTCTATGT TCCGGGAGTC TGGCTTCCAG GACAAGGAGG AGCTGACCTG GGAGGACTTC 
CACTTCATGC TGCGGGACCA CGACAGTGAT CTCCGATTCA CACAGCTCTG TGTCAAAGGT 
GTGGAAGTGC CGGAAGTGAT CAAGAACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAG 
AAGATCTGCC CTTCGCCCAG AATGAGCGCC CACTGTGCCC GAAACAACAT GAAGACTGCG 
TCATCACCAC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCTCAAGA GATTCGACGG 
AGGTTTGGCA AAAAGGTAAC TTCATTCCAG CCCTTACTGT TCACTGAGGC ACACCGAGAG 
AAGTTTCAAC GCAGCCGCCG CCACCAAACG GTGCAGCAGT TCAAGCGCTT TATTGAGAAT 
TACAGGCGCC ACATTGGCTG TGTGGCGGTG TTTTACACCA TCACTGGGGC GCTCTTCCTG 
GAGAGAGCCT ACTACTATGC ATTTGCAGCA CACCACAGCG GCATCACCGA TACCACCCGT 
GTAGGCATCA TCCTGTCCCG GGGCACGGCG GCCAGCATCT CCTTTATGTT TTCCTACATC 
CTGCTCACCA TGTGTCGCAA CCTCATCACC TTCTTGCGGG AGACCTTCCT CAACCGCTAC 
ATTCCCTTCG ATGCTGCCGT GGACTTCCAT CGCCTCATCG CCTCCACGGC CATCATCCTC 
ACAGTCTTAC ACAGTGCTGG CCACGTAGTG AATGTGTACC TGTTCTCCAT CAGTCCACTC 
AGTGTCCTCT CTTGCCTCTT CCCTGGCCTC TTCCACGATG ATGGGTCGGA GTTCCCTCAG 
AAGTATTACT GGTGGTTCTT CCAGACTGTG CCAGGTCTCA CTGGGGTCCT CCTGCTCCTG 
GCCCTGGCCA TCATGTACGT CTTCGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCATCACCT CTACATTTTT CTCTACATTC TGCTCATCAT ACATGGCAGC 
TTTGCTCTGA TCCAGATGCC CCGGTTCCAC ATCTTCTTCC TGGTCCCTGC AATTATTTAT 
GTGGGGGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCT 
GAGCTGCTGC CATCAGGAGT GACCCACCTG CGGTTCCAGA GGCCTCAGGG CTTTGAGTAT 
AAGTCAGGGC AGTGGGTTCG GATTGCGTGC CTGGCTCTGG GAACCACCGA GTATCACCCT 
TTCACACTGA CTTCTGCACC CCACGAGGAC ACACTCAGCC TGCACATCCG GGCGGCTGGA 
CCCTGGACCA CTCGCCTCAG GGAAATCTAC TCACCCCCAA CGGGTGACAC CTGTGCCAGA 
TACCCAAAGC TGTACCTCGA TGGACCGTTT GGAGAGGGCC ACCAGGAGTG GCACAAGTTT 
GAGGTGTCAG TGCTGGTAGG AGGGGGCATT GGAGTCACTC CCTTTGCCTC CATCCTCAAA 
GACCTGGTTT TCAAGTCATC AGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACAA GGACTCAGAG GCAGTTTGAG TGGCTGGCTG ACATCATCCG GGAGGTGGAG 
GAGAATGACC GCCAGGACCT GGTGTCTGTG CACATCTATA TTACTCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACCAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTGCTGAAC 
AGGAGTCTGT TCACGGGCCT GCGCTCCATC ACCCACTTTG GTCGCCCTCC CTTTGAGCCC 
TTTTTCAACT CTCTGCAGGA AGTCCACCCC CAAGTCCGAA AGATTGGAGT ATTTAGCTGT 
GGTCCCCCTG GCATGACCAA GAATGTGGAG AAGGCTTGTC AGCTCATCAA CAGGCAGGAC 
AGGACTCATT TTTCTCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092767.1
CDS	32..4687
Translation	MGFHLALAWILLVGTLASLGAQNSISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLKEPYLPNPRHLSNRVMRGSAGQPSLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIYIPHGDPVFDPDKRGNVVLPFQRSRWDRNTGQSPSNPRDQSNQVTGWLDGSAIYGSSHSWSDTLRSFSGGQLASGPDPAFPSDSQSSLLMWMAPDPSTGQGGPRGVYAFGAQRGNREPFLQALGLLWFRYHNLCARKLAQEHPHWGDEELFQHARKRVIATYQNIAMYEWLPSFLKQTPPEYPGYRPFLDPSISPEFVVASEQFLSTMVPSGVYMRNASCHFQGIPSHNSSVSGALRVCNSYWSREHPKLQRAEDVDALLLGMASQIAEREDHVVVEDMQDFWPGPLKFSRTDYLASCLQRGRDLGLPSYTKAREALGLSPISHWQDINPALSRSNGTVLEATAALYNQDLSRLELLPGGLLESHGDPGPLFSTIVLDQFVRLRDGDRYWFENTRNGLFSKEEIAEIRNTSLRDILVAVTNVDPSALQPNVFFWLAGDPCPQPSQLSAKGLPACAPLFIRDYFEGSGFGFGLTIGTLCCFPLVSLLSAWIVARLRKRNFKRLQRQDRQSIMSEKLVGGVEALEWQGRNEPCRPVLVHLQPGQIRVVDGRLTVLRTIQLRPPQQVNLILSSNRGRRTLLLKIPKEYDLVLLFNMEEERQALVENVRGALKENGLSFQEWELREQELMRAAVTRQQRGHLLETFFRHLFSQVLDINQADAGTLPLDSSTKVREALTCELSRAEFADSLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHDSDLRFTQLCVKGVEVPEVIKNLCRRASYISQEKICPSPRMSAHCARNNMKTASSPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSRRHQTVQQFKRFIENYRRHIGCVAVFYTITGALFLERAYYYAFAAHHSGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYIPFDAAVDFHRLIASTAIILTVLHSAGHVVNVYLFSISPLSVLSCLFPGLFHDDGSEFPQKYYWWFFQTVPGLTGVLLLLALAIMYVFASHHFRRRSFRGFWLTHHLYIFLYILLIIHGSFALIQMPRFHIFFLVPAIIYVGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSPPTGDTCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDRQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF

Target ORF information:

RefSeq Version	NM_001099297.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus dual oxidase 1 (Duox1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099297.1

ORF Insert Sequence:

ATGGGGTTCC ACTTAGCTCT GGCCTGGATT CTCCTGGTTG GGACACTGGC TTCTTTGGGA 
GCTCAGAACT CCATTTCTTG GGAGGTACAG CGATTTGATG GATGGTATAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCTCGG CTGCAGCGCC TTGTTCCAGC CAGCTATGCA 
GATGGTGTCT ACCAGCCCCT GAAAGAACCC TACCTGCCTA ACCCCCGACA CCTTAGTAAC 
AGGGTCATGA GAGGTTCTGC AGGGCAGCCA TCTCTTCGGA ACCGTACAGT CCTGGGTGTC 
TTCTTTGGCT ACCACGTGCT CTCAGACCTG GTGAGTGTGG AAACACCAGG CTGCCCCGCA 
GAGTTCCTCA ACATTTACAT CCCGCATGGG GACCCGGTGT TCGACCCTGA CAAGCGCGGG 
AACGTGGTGC TGCCCTTTCA AAGGAGCCGC TGGGACCGCA ACACTGGACA GAGCCCCAGC 
AACCCCCGGG ACCAGAGCAA CCAGGTGACC GGCTGGCTAG ATGGCAGCGC CATCTATGGC 
TCCTCTCATT CCTGGAGTGA CACTCTGAGG AGCTTCTCTG GAGGACAGCT GGCTTCTGGG 
CCTGACCCTG CTTTCCCATC GGACTCCCAG AGCTCTCTGC TCATGTGGAT GGCGCCGGAC 
CCCTCCACAG GGCAGGGAGG GCCACGGGGG GTGTATGCCT TCGGGGCCCA GCGCGGGAAC 
AGGGAGCCCT TTCTGCAGGC TCTGGGCTTG TTGTGGTTTC GCTACCACAA CCTGTGTGCC 
AGGAAGCTGG CGCAGGAGCA CCCGCACTGG GGGGATGAGG AACTGTTCCA GCATGCTCGC 
AAGAGGGTCA TTGCCACCTA CCAGAACATT GCGATGTATG AGTGGCTGCC CAGCTTCCTG 
AAGCAAACTC CTCCGGAGTA TCCAGGATAC CGTCCATTTC TGGACCCCAG TATCTCCCCA 
GAGTTCGTTG TGGCCTCTGA GCAGTTCCTC TCTACCATGG TGCCCTCTGG GGTCTACATG 
AGGAATGCCA GTTGTCATTT CCAGGGGATT CCCAGTCATA ACTCAAGTGT CTCTGGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGTCGA GAGCACCCCA AGCTACAAAG GGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATTGCTG AGAGAGAGGA CCACGTGGTG 
GTTGAGGACA TGCAAGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGTAC AGACTACCTG 
GCCAGCTGCC TGCAGAGGGG CCGGGATCTA GGCCTGCCTT CTTACACCAA AGCCAGGGAG 
GCACTGGGCC TGTCTCCCAT TTCCCACTGG CAGGACATCA ACCCTGCACT CTCCAGGAGC 
AATGGCACTG TGCTGGAGGC CACAGCTGCC CTGTACAATC AGGACCTGTC TCGGCTGGAG 
CTACTCCCAG GGGGGCTTCT GGAGAGCCAT GGAGACCCTG GACCCCTTTT CAGCACTATT 
GTCCTTGACC AGTTTGTCAG GCTACGAGAT GGTGACCGTT ACTGGTTTGA AAACACCAGG 
AACGGATTGT TCTCTAAGGA AGAGATCGCA GAGATCCGAA ACACTTCCCT ACGGGACATT 
CTAGTGGCTG TCACCAATGT GGACCCCAGT GCCCTACAGC CCAATGTCTT CTTTTGGCTT 
GCAGGAGACC CCTGTCCACA GCCAAGTCAG CTCAGCGCCA AGGGCCTGCC AGCCTGTGCC 
CCCCTCTTCA TTCGGGACTA TTTCGAGGGG AGTGGATTTG GCTTCGGACT CACCATTGGG 
ACCCTTTGCT GTTTCCCCTT AGTCAGCCTC CTTAGTGCCT GGATTGTTGC CCGGCTTCGA 
AAGAGGAATT TCAAGAGGCT CCAGAGACAA GACCGCCAGA GCATCATGTC GGAGAAGCTT 
GTAGGGGGAG TGGAAGCTTT GGAGTGGCAG GGCCGCAATG AACCCTGCAG GCCAGTGCTT 
GTGCACTTAC AGCCGGGACA GATTCGAGTA GTGGATGGCA GGCTCACCGT GCTCCGGACC 
ATCCAGCTGC GACCCCCACA GCAGGTCAAC CTCATCCTGT CCAGCAACCG TGGACGCCGC 
ACCCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTTC TGTTGTTTAA CATGGAGGAG 
GAACGGCAGG CACTGGTGGA AAACGTCAGG GGAGCTCTGA AGGAAAATGG GCTGAGCTTC 
CAGGAGTGGG AACTGAGGGA GCAGGAGCTG ATGAGGGCAG CAGTGACCAG GCAACAACGG 
GGCCACCTCC TGGAGACCTT CTTCAGGCAC CTTTTCTCCC AGGTGTTAGA CATCAATCAG 
GCTGATGCAG GGACTCTGCC TCTGGATTCC TCGACCAAAG TGCGTGAGGC CCTGACCTGT 
GAGCTGAGCA GAGCTGAGTT TGCAGACTCC CTAGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TTTCTCTAGC CGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGGGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCCCCAGAGG AGAAGTCTCG TCTTATGTTC 
CGAATGTATG ACTTTGATGG GAACGGTCTC ATTTCCAAGG ATGAGTTCAT TAGGATGCTA 
AGGTCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTT 
GAGTCTATGT TCCGGGAGTC TGGCTTCCAG GACAAGGAGG AGCTGACCTG GGAGGACTTC 
CACTTCATGC TGCGGGACCA CGACAGTGAT CTCCGATTCA CACAGCTCTG TGTCAAAGGT 
GTGGAAGTGC CGGAAGTGAT CAAGAACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAG 
AAGATCTGCC CTTCGCCCAG AATGAGCGCC CACTGTGCCC GAAACAACAT GAAGACTGCG 
TCATCACCAC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCTCAAGA GATTCGACGG 
AGGTTTGGCA AAAAGGTAAC TTCATTCCAG CCCTTACTGT TCACTGAGGC ACACCGAGAG 
AAGTTTCAAC GCAGCCGCCG CCACCAAACG GTGCAGCAGT TCAAGCGCTT TATTGAGAAT 
TACAGGCGCC ACATTGGCTG TGTGGCGGTG TTTTACACCA TCACTGGGGC GCTCTTCCTG 
GAGAGAGCCT ACTACTATGC ATTTGCAGCA CACCACAGCG GCATCACCGA TACCACCCGT 
GTAGGCATCA TCCTGTCCCG GGGCACGGCG GCCAGCATCT CCTTTATGTT TTCCTACATC 
CTGCTCACCA TGTGTCGCAA CCTCATCACC TTCTTGCGGG AGACCTTCCT CAACCGCTAC 
ATTCCCTTCG ATGCTGCCGT GGACTTCCAT CGCCTCATCG CCTCCACGGC CATCATCCTC 
ACAGTCTTAC ACAGTGCTGG CCACGTAGTG AATGTGTACC TGTTCTCCAT CAGTCCACTC 
AGTGTCCTCT CTTGCCTCTT CCCTGGCCTC TTCCACGATG ATGGGTCGGA GTTCCCTCAG 
AAGTATTACT GGTGGTTCTT CCAGACTGTG CCAGGTCTCA CTGGGGTCCT CCTGCTCCTG 
GCCCTGGCCA TCATGTACGT CTTCGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCATCACCT CTACATTTTT CTCTACATTC TGCTCATCAT ACATGGCAGC 
TTTGCTCTGA TCCAGATGCC CCGGTTCCAC ATCTTCTTCC TGGTCCCTGC AATTATTTAT 
GTGGGGGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCT 
GAGCTGCTGC CATCAGGAGT GACCCACCTG CGGTTCCAGA GGCCTCAGGG CTTTGAGTAT 
AAGTCAGGGC AGTGGGTTCG GATTGCGTGC CTGGCTCTGG GAACCACCGA GTATCACCCT 
TTCACACTGA CTTCTGCACC CCACGAGGAC ACACTCAGCC TGCACATCCG GGCGGCTGGA 
CCCTGGACCA CTCGCCTCAG GGAAATCTAC TCACCCCCAA CGGGTGACAC CTGTGCCAGA 
TACCCAAAGC TGTACCTCGA TGGACCGTTT GGAGAGGGCC ACCAGGAGTG GCACAAGTTT 
GAGGTGTCAG TGCTGGTAGG AGGGGGCATT GGAGTCACTC CCTTTGCCTC CATCCTCAAA 
GACCTGGTTT TCAAGTCATC AGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACAA GGACTCAGAG GCAGTTTGAG TGGCTGGCTG ACATCATCCG GGAGGTGGAG 
GAGAATGACC GCCAGGACCT GGTGTCTGTG CACATCTATA TTACTCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACCAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTGCTGAAC 
AGGAGTCTGT TCACGGGCCT GCGCTCCATC ACCCACTTTG GTCGCCCTCC CTTTGAGCCC 
TTTTTCAACT CTCTGCAGGA AGTCCACCCC CAAGTCCGAA AGATTGGAGT ATTTAGCTGT 
GGTCCCCCTG GCATGACCAA GAATGTGGAG AAGGCTTGTC AGCTCATCAA CAGGCAGGAC 
AGGACTCATT TTTCTCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Duox1 ( XM_006500487.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006500550.1 Mus musculus dual oxidase 1 (Duox1), transcript variant X1, mRNA.

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CloneID	OMu03260
Clone ID Related Accession (Same CDS sequence)	XM_030252279.1 , XM_006500487.3 , NM_001099297.1 , XM_006500487.2
Accession Version	XM_006500487.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	dual oxidase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTCC ACTTAGCTCT GGCCTGGATT CTCCTGGTTG GGACACTGGC TTCTTTGGGA 
GCTCAGAACT CCATTTCTTG GGAGGTACAG CGATTTGATG GATGGTATAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCTCGG CTGCAGCGCC TTGTTCCAGC CAGCTATGCA 
GATGGTGTCT ACCAGCCCCT GAAAGAACCC TACCTGCCTA ACCCCCGACA CCTTAGTAAC 
AGGGTCATGA GAGGTTCTGC AGGGCAGCCA TCTCTTCGGA ACCGTACAGT CCTGGGTGTC 
TTCTTTGGCT ACCACGTGCT CTCAGACCTG GTGAGTGTGG AAACACCAGG CTGCCCCGCA 
GAGTTCCTCA ACATTTACAT CCCGCATGGG GACCCGGTGT TCGACCCTGA CAAGCGCGGG 
AACGTGGTGC TGCCCTTTCA AAGGAGCCGC TGGGACCGCA ACACTGGACA GAGCCCCAGC 
AACCCCCGGG ACCAGAGCAA CCAGGTGACC GGCTGGCTAG ATGGCAGCGC CATCTATGGC 
TCCTCTCATT CCTGGAGTGA CACTCTGAGG AGCTTCTCTG GAGGACAGCT GGCTTCTGGG 
CCTGACCCTG CTTTCCCATC GGACTCCCAG AGCTCTCTGC TCATGTGGAT GGCGCCGGAC 
CCCTCCACAG GGCAGGGAGG GCCACGGGGG GTGTATGCCT TCGGGGCCCA GCGCGGGAAC 
AGGGAGCCCT TTCTGCAGGC TCTGGGCTTG TTGTGGTTTC GCTACCACAA CCTGTGTGCC 
AGGAAGCTGG CGCAGGAGCA CCCGCACTGG GGGGATGAGG AACTGTTCCA GCATGCTCGC 
AAGAGGGTCA TTGCCACCTA CCAGAACATT GCGATGTATG AGTGGCTGCC CAGCTTCCTG 
AAGCAAACTC CTCCGGAGTA TCCAGGATAC CGTCCATTTC TGGACCCCAG TATCTCCCCA 
GAGTTCGTTG TGGCCTCTGA GCAGTTCCTC TCTACCATGG TGCCCTCTGG GGTCTACATG 
AGGAATGCCA GTTGTCATTT CCAGGGGATT CCCAGTCATA ACTCAAGTGT CTCTGGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGTCGA GAGCACCCCA AGCTACAAAG GGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATTGCTG AGAGAGAGGA CCACGTGGTG 
GTTGAGGACA TGCAAGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGTAC AGACTACCTG 
GCCAGCTGCC TGCAGAGGGG CCGGGATCTA GGCCTGCCTT CTTACACCAA AGCCAGGGAG 
GCACTGGGCC TGTCTCCCAT TTCCCACTGG CAGGACATCA ACCCTGCACT CTCCAGGAGC 
AATGGCACTG TGCTGGAGGC CACAGCTGCC CTGTACAATC AGGACCTGTC TCGGCTGGAG 
CTACTCCCAG GGGGGCTTCT GGAGAGCCAT GGAGACCCTG GACCCCTTTT CAGCACTATT 
GTCCTTGACC AGTTTGTCAG GCTACGAGAT GGTGACCGTT ACTGGTTTGA AAACACCAGG 
AACGGATTGT TCTCTAAGGA AGAGATCGCA GAGATCCGAA ACACTTCCCT ACGGGACATT 
CTAGTGGCTG TCACCAATGT GGACCCCAGT GCCCTACAGC CCAATGTCTT CTTTTGGCTT 
GCAGGAGACC CCTGTCCACA GCCAAGTCAG CTCAGCGCCA AGGGCCTGCC AGCCTGTGCC 
CCCCTCTTCA TTCGGGACTA TTTCGAGGGG AGTGGATTTG GCTTCGGACT CACCATTGGG 
ACCCTTTGCT GTTTCCCCTT AGTCAGCCTC CTTAGTGCCT GGATTGTTGC CCGGCTTCGA 
AAGAGGAATT TCAAGAGGCT CCAGAGACAA GACCGCCAGA GCATCATGTC GGAGAAGCTT 
GTAGGGGGAG TGGAAGCTTT GGAGTGGCAG GGCCGCAATG AACCCTGCAG GCCAGTGCTT 
GTGCACTTAC AGCCGGGACA GATTCGAGTA GTGGATGGCA GGCTCACCGT GCTCCGGACC 
ATCCAGCTGC GACCCCCACA GCAGGTCAAC CTCATCCTGT CCAGCAACCG TGGACGCCGC 
ACCCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTTC TGTTGTTTAA CATGGAGGAG 
GAACGGCAGG CACTGGTGGA AAACGTCAGG GGAGCTCTGA AGGAAAATGG GCTGAGCTTC 
CAGGAGTGGG AACTGAGGGA GCAGGAGCTG ATGAGGGCAG CAGTGACCAG GCAACAACGG 
GGCCACCTCC TGGAGACCTT CTTCAGGCAC CTTTTCTCCC AGGTGTTAGA CATCAATCAG 
GCTGATGCAG GGACTCTGCC TCTGGATTCC TCGACCAAAG TGCGTGAGGC CCTGACCTGT 
GAGCTGAGCA GAGCTGAGTT TGCAGACTCC CTAGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TTTCTCTAGC CGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGGGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCCCCAGAGG AGAAGTCTCG TCTTATGTTC 
CGAATGTATG ACTTTGATGG GAACGGTCTC ATTTCCAAGG ATGAGTTCAT TAGGATGCTA 
AGGTCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTT 
GAGTCTATGT TCCGGGAGTC TGGCTTCCAG GACAAGGAGG AGCTGACCTG GGAGGACTTC 
CACTTCATGC TGCGGGACCA CGACAGTGAT CTCCGATTCA CACAGCTCTG TGTCAAAGGT 
GTGGAAGTGC CGGAAGTGAT CAAGAACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAG 
AAGATCTGCC CTTCGCCCAG AATGAGCGCC CACTGTGCCC GAAACAACAT GAAGACTGCG 
TCATCACCAC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCTCAAGA GATTCGACGG 
AGGTTTGGCA AAAAGGTAAC TTCATTCCAG CCCTTACTGT TCACTGAGGC ACACCGAGAG 
AAGTTTCAAC GCAGCCGCCG CCACCAAACG GTGCAGCAGT TCAAGCGCTT TATTGAGAAT 
TACAGGCGCC ACATTGGCTG TGTGGCGGTG TTTTACACCA TCACTGGGGC GCTCTTCCTG 
GAGAGAGCCT ACTACTATGC ATTTGCAGCA CACCACAGCG GCATCACCGA TACCACCCGT 
GTAGGCATCA TCCTGTCCCG GGGCACGGCG GCCAGCATCT CCTTTATGTT TTCCTACATC 
CTGCTCACCA TGTGTCGCAA CCTCATCACC TTCTTGCGGG AGACCTTCCT CAACCGCTAC 
ATTCCCTTCG ATGCTGCCGT GGACTTCCAT CGCCTCATCG CCTCCACGGC CATCATCCTC 
ACAGTCTTAC ACAGTGCTGG CCACGTAGTG AATGTGTACC TGTTCTCCAT CAGTCCACTC 
AGTGTCCTCT CTTGCCTCTT CCCTGGCCTC TTCCACGATG ATGGGTCGGA GTTCCCTCAG 
AAGTATTACT GGTGGTTCTT CCAGACTGTG CCAGGTCTCA CTGGGGTCCT CCTGCTCCTG 
GCCCTGGCCA TCATGTACGT CTTCGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCATCACCT CTACATTTTT CTCTACATTC TGCTCATCAT ACATGGCAGC 
TTTGCTCTGA TCCAGATGCC CCGGTTCCAC ATCTTCTTCC TGGTCCCTGC AATTATTTAT 
GTGGGGGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCT 
GAGCTGCTGC CATCAGGAGT GACCCACCTG CGGTTCCAGA GGCCTCAGGG CTTTGAGTAT 
AAGTCAGGGC AGTGGGTTCG GATTGCGTGC CTGGCTCTGG GAACCACCGA GTATCACCCT 
TTCACACTGA CTTCTGCACC CCACGAGGAC ACACTCAGCC TGCACATCCG GGCGGCTGGA 
CCCTGGACCA CTCGCCTCAG GGAAATCTAC TCACCCCCAA CGGGTGACAC CTGTGCCAGA 
TACCCAAAGC TGTACCTCGA TGGACCGTTT GGAGAGGGCC ACCAGGAGTG GCACAAGTTT 
GAGGTGTCAG TGCTGGTAGG AGGGGGCATT GGAGTCACTC CCTTTGCCTC CATCCTCAAA 
GACCTGGTTT TCAAGTCATC AGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACAA GGACTCAGAG GCAGTTTGAG TGGCTGGCTG ACATCATCCG GGAGGTGGAG 
GAGAATGACC GCCAGGACCT GGTGTCTGTG CACATCTATA TTACTCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACCAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTGCTGAAC 
AGGAGTCTGT TCACGGGCCT GCGCTCCATC ACCCACTTTG GTCGCCCTCC CTTTGAGCCC 
TTTTTCAACT CTCTGCAGGA AGTCCACCCC CAAGTCCGAA AGATTGGAGT ATTTAGCTGT 
GGTCCCCCTG GCATGACCAA GAATGTGGAG AAGGCTTGTC AGCTCATCAA CAGGCAGGAC 
AGGACTCATT TTTCTCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006500550.1
CDS	138..4793
Translation	MGFHLALAWILLVGTLASLGAQNSISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLKEPYLPNPRHLSNRVMRGSAGQPSLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIYIPHGDPVFDPDKRGNVVLPFQRSRWDRNTGQSPSNPRDQSNQVTGWLDGSAIYGSSHSWSDTLRSFSGGQLASGPDPAFPSDSQSSLLMWMAPDPSTGQGGPRGVYAFGAQRGNREPFLQALGLLWFRYHNLCARKLAQEHPHWGDEELFQHARKRVIATYQNIAMYEWLPSFLKQTPPEYPGYRPFLDPSISPEFVVASEQFLSTMVPSGVYMRNASCHFQGIPSHNSSVSGALRVCNSYWSREHPKLQRAEDVDALLLGMASQIAEREDHVVVEDMQDFWPGPLKFSRTDYLASCLQRGRDLGLPSYTKAREALGLSPISHWQDINPALSRSNGTVLEATAALYNQDLSRLELLPGGLLESHGDPGPLFSTIVLDQFVRLRDGDRYWFENTRNGLFSKEEIAEIRNTSLRDILVAVTNVDPSALQPNVFFWLAGDPCPQPSQLSAKGLPACAPLFIRDYFEGSGFGFGLTIGTLCCFPLVSLLSAWIVARLRKRNFKRLQRQDRQSIMSEKLVGGVEALEWQGRNEPCRPVLVHLQPGQIRVVDGRLTVLRTIQLRPPQQVNLILSSNRGRRTLLLKIPKEYDLVLLFNMEEERQALVENVRGALKENGLSFQEWELREQELMRAAVTRQQRGHLLETFFRHLFSQVLDINQADAGTLPLDSSTKVREALTCELSRAEFADSLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHDSDLRFTQLCVKGVEVPEVIKNLCRRASYISQEKICPSPRMSAHCARNNMKTASSPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSRRHQTVQQFKRFIENYRRHIGCVAVFYTITGALFLERAYYYAFAAHHSGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYIPFDAAVDFHRLIASTAIILTVLHSAGHVVNVYLFSISPLSVLSCLFPGLFHDDGSEFPQKYYWWFFQTVPGLTGVLLLLALAIMYVFASHHFRRRSFRGFWLTHHLYIFLYILLIIHGSFALIQMPRFHIFFLVPAIIYVGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSPPTGDTCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDRQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF

Target ORF information:

RefSeq Version	XM_006500487.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dual oxidase 1 (Duox1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006500487.2

ORF Insert Sequence:

ATGGGGTTCC ACTTAGCTCT GGCCTGGATT CTCCTGGTTG GGACACTGGC TTCTTTGGGA 
GCTCAGAACT CCATTTCTTG GGAGGTACAG CGATTTGATG GATGGTATAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCTCGG CTGCAGCGCC TTGTTCCAGC CAGCTATGCA 
GATGGTGTCT ACCAGCCCCT GAAAGAACCC TACCTGCCTA ACCCCCGACA CCTTAGTAAC 
AGGGTCATGA GAGGTTCTGC AGGGCAGCCA TCTCTTCGGA ACCGTACAGT CCTGGGTGTC 
TTCTTTGGCT ACCACGTGCT CTCAGACCTG GTGAGTGTGG AAACACCAGG CTGCCCCGCA 
GAGTTCCTCA ACATTTACAT CCCGCATGGG GACCCGGTGT TCGACCCTGA CAAGCGCGGG 
AACGTGGTGC TGCCCTTTCA AAGGAGCCGC TGGGACCGCA ACACTGGACA GAGCCCCAGC 
AACCCCCGGG ACCAGAGCAA CCAGGTGACC GGCTGGCTAG ATGGCAGCGC CATCTATGGC 
TCCTCTCATT CCTGGAGTGA CACTCTGAGG AGCTTCTCTG GAGGACAGCT GGCTTCTGGG 
CCTGACCCTG CTTTCCCATC GGACTCCCAG AGCTCTCTGC TCATGTGGAT GGCGCCGGAC 
CCCTCCACAG GGCAGGGAGG GCCACGGGGG GTGTATGCCT TCGGGGCCCA GCGCGGGAAC 
AGGGAGCCCT TTCTGCAGGC TCTGGGCTTG TTGTGGTTTC GCTACCACAA CCTGTGTGCC 
AGGAAGCTGG CGCAGGAGCA CCCGCACTGG GGGGATGAGG AACTGTTCCA GCATGCTCGC 
AAGAGGGTCA TTGCCACCTA CCAGAACATT GCGATGTATG AGTGGCTGCC CAGCTTCCTG 
AAGCAAACTC CTCCGGAGTA TCCAGGATAC CGTCCATTTC TGGACCCCAG TATCTCCCCA 
GAGTTCGTTG TGGCCTCTGA GCAGTTCCTC TCTACCATGG TGCCCTCTGG GGTCTACATG 
AGGAATGCCA GTTGTCATTT CCAGGGGATT CCCAGTCATA ACTCAAGTGT CTCTGGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGTCGA GAGCACCCCA AGCTACAAAG GGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATTGCTG AGAGAGAGGA CCACGTGGTG 
GTTGAGGACA TGCAAGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGTAC AGACTACCTG 
GCCAGCTGCC TGCAGAGGGG CCGGGATCTA GGCCTGCCTT CTTACACCAA AGCCAGGGAG 
GCACTGGGCC TGTCTCCCAT TTCCCACTGG CAGGACATCA ACCCTGCACT CTCCAGGAGC 
AATGGCACTG TGCTGGAGGC CACAGCTGCC CTGTACAATC AGGACCTGTC TCGGCTGGAG 
CTACTCCCAG GGGGGCTTCT GGAGAGCCAT GGAGACCCTG GACCCCTTTT CAGCACTATT 
GTCCTTGACC AGTTTGTCAG GCTACGAGAT GGTGACCGTT ACTGGTTTGA AAACACCAGG 
AACGGATTGT TCTCTAAGGA AGAGATCGCA GAGATCCGAA ACACTTCCCT ACGGGACATT 
CTAGTGGCTG TCACCAATGT GGACCCCAGT GCCCTACAGC CCAATGTCTT CTTTTGGCTT 
GCAGGAGACC CCTGTCCACA GCCAAGTCAG CTCAGCGCCA AGGGCCTGCC AGCCTGTGCC 
CCCCTCTTCA TTCGGGACTA TTTCGAGGGG AGTGGATTTG GCTTCGGACT CACCATTGGG 
ACCCTTTGCT GTTTCCCCTT AGTCAGCCTC CTTAGTGCCT GGATTGTTGC CCGGCTTCGA 
AAGAGGAATT TCAAGAGGCT CCAGAGACAA GACCGCCAGA GCATCATGTC GGAGAAGCTT 
GTAGGGGGAG TGGAAGCTTT GGAGTGGCAG GGCCGCAATG AACCCTGCAG GCCAGTGCTT 
GTGCACTTAC AGCCGGGACA GATTCGAGTA GTGGATGGCA GGCTCACCGT GCTCCGGACC 
ATCCAGCTGC GACCCCCACA GCAGGTCAAC CTCATCCTGT CCAGCAACCG TGGACGCCGC 
ACCCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTTC TGTTGTTTAA CATGGAGGAG 
GAACGGCAGG CACTGGTGGA AAACGTCAGG GGAGCTCTGA AGGAAAATGG GCTGAGCTTC 
CAGGAGTGGG AACTGAGGGA GCAGGAGCTG ATGAGGGCAG CAGTGACCAG GCAACAACGG 
GGCCACCTCC TGGAGACCTT CTTCAGGCAC CTTTTCTCCC AGGTGTTAGA CATCAATCAG 
GCTGATGCAG GGACTCTGCC TCTGGATTCC TCGACCAAAG TGCGTGAGGC CCTGACCTGT 
GAGCTGAGCA GAGCTGAGTT TGCAGACTCC CTAGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TTTCTCTAGC CGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGGGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCCCCAGAGG AGAAGTCTCG TCTTATGTTC 
CGAATGTATG ACTTTGATGG GAACGGTCTC ATTTCCAAGG ATGAGTTCAT TAGGATGCTA 
AGGTCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTT 
GAGTCTATGT TCCGGGAGTC TGGCTTCCAG GACAAGGAGG AGCTGACCTG GGAGGACTTC 
CACTTCATGC TGCGGGACCA CGACAGTGAT CTCCGATTCA CACAGCTCTG TGTCAAAGGT 
GTGGAAGTGC CGGAAGTGAT CAAGAACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAG 
AAGATCTGCC CTTCGCCCAG AATGAGCGCC CACTGTGCCC GAAACAACAT GAAGACTGCG 
TCATCACCAC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCTCAAGA GATTCGACGG 
AGGTTTGGCA AAAAGGTAAC TTCATTCCAG CCCTTACTGT TCACTGAGGC ACACCGAGAG 
AAGTTTCAAC GCAGCCGCCG CCACCAAACG GTGCAGCAGT TCAAGCGCTT TATTGAGAAT 
TACAGGCGCC ACATTGGCTG TGTGGCGGTG TTTTACACCA TCACTGGGGC GCTCTTCCTG 
GAGAGAGCCT ACTACTATGC ATTTGCAGCA CACCACAGCG GCATCACCGA TACCACCCGT 
GTAGGCATCA TCCTGTCCCG GGGCACGGCG GCCAGCATCT CCTTTATGTT TTCCTACATC 
CTGCTCACCA TGTGTCGCAA CCTCATCACC TTCTTGCGGG AGACCTTCCT CAACCGCTAC 
ATTCCCTTCG ATGCTGCCGT GGACTTCCAT CGCCTCATCG CCTCCACGGC CATCATCCTC 
ACAGTCTTAC ACAGTGCTGG CCACGTAGTG AATGTGTACC TGTTCTCCAT CAGTCCACTC 
AGTGTCCTCT CTTGCCTCTT CCCTGGCCTC TTCCACGATG ATGGGTCGGA GTTCCCTCAG 
AAGTATTACT GGTGGTTCTT CCAGACTGTG CCAGGTCTCA CTGGGGTCCT CCTGCTCCTG 
GCCCTGGCCA TCATGTACGT CTTCGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCATCACCT CTACATTTTT CTCTACATTC TGCTCATCAT ACATGGCAGC 
TTTGCTCTGA TCCAGATGCC CCGGTTCCAC ATCTTCTTCC TGGTCCCTGC AATTATTTAT 
GTGGGGGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCT 
GAGCTGCTGC CATCAGGAGT GACCCACCTG CGGTTCCAGA GGCCTCAGGG CTTTGAGTAT 
AAGTCAGGGC AGTGGGTTCG GATTGCGTGC CTGGCTCTGG GAACCACCGA GTATCACCCT 
TTCACACTGA CTTCTGCACC CCACGAGGAC ACACTCAGCC TGCACATCCG GGCGGCTGGA 
CCCTGGACCA CTCGCCTCAG GGAAATCTAC TCACCCCCAA CGGGTGACAC CTGTGCCAGA 
TACCCAAAGC TGTACCTCGA TGGACCGTTT GGAGAGGGCC ACCAGGAGTG GCACAAGTTT 
GAGGTGTCAG TGCTGGTAGG AGGGGGCATT GGAGTCACTC CCTTTGCCTC CATCCTCAAA 
GACCTGGTTT TCAAGTCATC AGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACAA GGACTCAGAG GCAGTTTGAG TGGCTGGCTG ACATCATCCG GGAGGTGGAG 
GAGAATGACC GCCAGGACCT GGTGTCTGTG CACATCTATA TTACTCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACCAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTGCTGAAC 
AGGAGTCTGT TCACGGGCCT GCGCTCCATC ACCCACTTTG GTCGCCCTCC CTTTGAGCCC 
TTTTTCAACT CTCTGCAGGA AGTCCACCCC CAAGTCCGAA AGATTGGAGT ATTTAGCTGT 
GGTCCCCCTG GCATGACCAA GAATGTGGAG AAGGCTTGTC AGCTCATCAA CAGGCAGGAC 
AGGACTCATT TTTCTCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autophagy regulates DUOX1 localization and superoxide production in airway epithelial cells during chronic IL-13 stimulation.
Redox biology14272-284(2018 Apr)
Dickinson JD,Sweeter JM,Warren KJ,Ahmad IM,De Deken X,Zimmerman MC,Brody SL

Cigarette Smoke Impairs A
Scientific reports744405(2017 Mar)
Tian Z,Zhang H,Dixon J,Traphagen N,Wyatt TA,Kharbanda K,Simet Chadwick S,Kolliputi N,Allen-Gipson DS

Overexpression of Interleukin-4 in the Thyroid of Transgenic Mice Upregulates the Expression of Duox1 and the Anion Transporter Pendrin.
Thyroid : official journal of the American Thyroid Association26(10)1499-1512(2016 Oct)
Eskalli Z,Achouri Y,Hahn S,Many MC,Craps J,Refetoff S,Liao XH,Dumont JE,Van Sande J,Corvilain B,Miot F,De Deken X

The NADPH Oxidases DUOX1 and NOX2 Play Distinct Roles in Redox Regulation of Epidermal Growth Factor Receptor Signaling.
The Journal of biological chemistry291(44)23282-23293(2016 10)
Heppner DE,Hristova M,Dustin CM,Danyal K,Habibovic A,van der Vliet A

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Duox1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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電話

FAX

オンラインフォーム

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