NCU00621 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU00621
Entrez Gene ID	3881958
Full Name	TORC1 growth control complex subunit Kog1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_960668.2	XP_965761.2	TORC1 growth control complex subunit Kog1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rptor	NP_083174.2
Rattus norvegicus (Norway rat)	Rptor	NP_001127971.2
Homo sapiens (human)	RPTOR	NP_065812.1
Canis lupus familiaris (dog)	RPTOR	XP_850487.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010035	XP_001689178.1
Kluyveromyces lactis	KLLA0A09471g	XP_451414.1
Magnaporthe oryzae (rice blast fungus)	MGG_02874	XP_003720858.1
Gallus gallus (chicken)	RPTOR	XP_426232.2
Drosophila melanogaster (fruit fly)	raptor	NP_572294.2
Saccharomyces cerevisiae (baker's yeast)	KOG1	NP_012056.1
Schizosaccharomyces pombe (fission yeast)	mip1	NP_593370.1
	RPTOR	XP_001110471.1
Neurospora crassa	NCU00621	XP_965761.1
Arabidopsis thaliana (thale cress)	RAPTOR2	NP_195797.3
Pan troglodytes (chimpanzee)	RPTOR	XP_003953266.1
Bos taurus (cattle)	RPTOR	NP_001179059.1
Danio rerio (zebrafish)	rptor	XP_005157411.1
Eremothecium gossypii	AGOS_ADR331C	NP_984427.2
Arabidopsis thaliana (thale cress)	RAPTOR1	NP_566335.1
Oryza sativa (rice)	Os12g0110000	NP_001065961.1
Xenopus tropicalis (tropical clawed frog)	rptor	NP_001123843.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU00621 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU00621 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf13330	XM_960668.2 Latest version!	Neurospora crassa OR74A TORC1 growth control complex subunit Kog1 (NCU00621), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU00621 ( XM_960668.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_965761.2 Neurospora crassa OR74A TORC1 growth control complex subunit Kog1 (NCU00621), mRNA.

Price & Availability↑

CloneID	ONf13330
Clone ID Related Accession (Same CDS sequence)	XM_960668.2
Accession Version	XM_960668.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	TORC1 growth control complex subunit Kog1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_960668.1.

ATGACTGCTG TTTCTCTTCA CGCCTCCCAC CCCGGCTCCG CAGAATTTGG CCAGAGACAG 
CCCTCTGCTC CCACTCCCAC CTCTGGCCGT TACGCGAGGC CCGGCTTTCC GTCATCAACT 
GCTACATCAC CATCCCGCCA CACAATGAAC AACCCAGCTC GTCAAAGTTC AGTCACAATG 
TCACAAGTCC ATCCTCAGAG GCCAGAAGAC GGCAACCAGC GCGTCGCGAC AAACGGGACC 
TCTCATCCTG CTGCCCATAA CCAGCTGCCA CTTCGATCCA ACATACAGAG ACGCATGTCC 
GATTCCTCAC CAGCGCCCTT GTTTCGACGC CCCTCCAGCG CCCCCGACGA CAATAACCAA 
ACATCTCCTG GAACCACGTT GCATGGCGGG CAATCTGATG ACGACGACCA TATGCCTGTC 
GCCCGGCCGG CCAAGGCGCC ATTAACGCGG TCTAAGAGCG AATACGGACT GCGGTTGGAG 
AGGGAAAGCC CCATCGAACA AGTTGACGAA GAGTACCATG ATTGGGGTGC CAGGCATGGG 
TTCGAGGACC ATTATCAGTC TGAAGAAGTC ATTTCTCAGC TTGCAAATAG CTGGTACATG 
TACTTTACCG ATAAGCGACA TGAGACAACA GGGAAGCCAA AGACACCCGC TTTCGAGATC 
CAAGACTGGC GTATGCGCGA TCGCCTAAAA ACAGTTTCTG CTGCGATAGC AGTATGCTTG 
AACATAGGAG TGGAACCGCC AGATCAACTT CGCACCACCC CGGGTGCCCG GCTTGAAGCT 
TGGCAAGATC CGACGGTGCC TCCTGTTTCC AAGGCACTGG AGAATATTGG GAAAGCTTTG 
CAATCTCAAT ATGAGACCCT TGCTATTCGG ACGCGCTACA AGCAGTATTT AGACCCGTCC 
ATCGAAGAAA CCAAGCGGTT CAGTGTCTCC CTCCGAAGAA GTGCCAAGGA CGAGCGTGTG 
CTCTTCCATT ACAATGGGCA TGGAGTGCCC AAGCCAACAG CCTCGGGAGA GATCTGGGTT 
TTCAACAAGA ACTACACGCA GTATATCCCC GTCTCTCTCT ACGACCTTCA GCAATGGTTG 
CAGGCGCCCA CGATATATGT GTGGGATTGC TCCGAGGCTG GCAATATCCT CACCAATTAC 
CACCGTTTTG TTGAGAAGCA CGAGCAAGAA GAAGAGGAGG CGGCACAACG GGACCCAAAC 
CACGAAAAGA TTAACTTCCG TCCCTACATC CATCTGGCAG CCTGCAACGT CAAGGAGAAT 
CTGCCAACCA ACCCCGACCT GCCTGCTGAT CTCTTCACTT GCTGCTTGAC TACCCCTATA 
GAGATGGCAC TCTGGTTCTT CTGTCTGCAA AATCCGCTAA AAACCAAAAT ATCTCCTGAG 
CGAGCCAAGA AGCTGCCAGG GCGGCTTCAG GAGCGCCGGA CGCCTCTCGG AGAGCTCAAC 
TGGATTTTCA CTGCCATCAC AGATACCATT GCATGGACCA CGCTACCACG CCATCTCTTC 
CGCAAATTTT TTAGGCAAGA TTTGATGGTT GCCGCTCTTT TCCGAAACTT TCTCCTCGCA 
CAGCGTATCA TGCCTGTGTA CAACTGCCAT CCCCAGTCGT ATCCAGAGTT GCCAGATACA 
CGTCAGCATC CCCTTTGGGA GAGCTGGGAC TTGGCCGTCG ATCTGGCTCT TGCCCAGTTG 
CCGGCCCTCG AGAGAAAGGA GAGTGAAGGG ATAGAGTATG AGTACATCAA CTCGTCATTC 
TTCGCGGAGC AGCTCACAGC ATTCGAGATC TACTTGACTC GAGGGGATGC TTTGTCCCAG 
AAGCCACCTG ACCAGCTTCC AGTGGTTCTG CAAGTGTTGC TAAGTCAGCA GCATCGAGTA 
CGAGCTCTGG TCCTACTAGC AAGGTTCTTG GACCTTGGCC CTTGGGCAGT ACAACTGGCG 
CTCAGCATTG GCATCTTCCC GTACGTCTTG AAGCTCCTCC AGTCGGCGGC ACAAGACCTA 
AAGCCAGTCA TGGTGTTCAT CTGGACTCGC GTTCTTGCGG TCGATATCTC ATGCCAGCAA 
GACCTCATCA AAGATAGCGG GTACAATTAC TTCGCGGCCA TAATGAAGCC GCAAGATACC 
CTCTCCATGG TTAGCGTCGG GTTCTTGGAT GAGCACAAGG CGATGTGTGC TTTCATCTTG 
GCTATGCTGT GCAAAGACTA TAAACCTGGA CAGCTTGTGT GTAACTCAAC TGACATTATG 
ACCTATTGCC TGTACCATAT TGCACGCCCA GAAGGCCCCC TCCTGAAGCA ATGGTCATGC 
CTCTGCATCA GCCAGCTCTG GAAGGACCTT CCTGAAGGTA AATGGAGGGG CATTCGGGAG 
AACGCCACTT CGAAACTCTC CCACCTTAGT AGGGACCCCA ACCCTGAAGT ACGGGCAGCT 
ATGGCGCATG CTATGACCAC GTTCCTAGGA ATCCCGGATC TCACGGACGA AGTTGCGAGG 
ATCGAGGAAA GCGTTGCCTG GACCATGCTT GAACTGGCTA CAGACGGCAG CCCGATTGTC 
CGAAAAGAGC TTCTAGTCTT CTTCTCTCAT TTCGTTCTGC GTTATGAGAG CAAGTTCCTG 
GTTGCCGCCT TCGAGCAACT ATCCGAGGAG AAGGATTATC TTGCTTCGCC TCCGCCAGAG 
GATGGCCAAG ATCACAGAAT GGGGTTACAC TATACTCGCC GAGAAAACCG GGAGAGAGAT 
GGCAGTATCA AACCATCAGC ATTTGGTGTA GCGCATGATT CGATCTTCGC TGCCTGCTGG 
AAACACATCA ACATCTTGAG CGTGGATCCA CATCCCGAAA CCCAAAGAGA TGCCACCATC 
ATCGTGGATT ATGTTCACAA TGCACTTCTT CAATCACCAC TTACAACGCA AACGCAAAAT 
CTCATGGACG AGATCCTCCG GCGCTCTAGA AAAGTGTCGA GGGGTGATAT GAGCCAGAGA 
AGCGCCGCTG CCGGCACTCA CGCTGCAGTC GCACAGCCAT TGCCTTCGCC AGGCCTTCTC 
AAGAGGACTG CAAGTTACCT GTTTGGCCCC CTCATGGGGA CCCTGGACCC TTCTTCAACC 
TCGATTCCGC CACTGTCCCC TGGGCTACAA AGAAGTGGAT CTCGAAGCAG CCGAAAAGGT 
CCGGGCCTGG ATTCTGCGCC ACCTGAGCAG AATGATCAGG CTACTTCGCC AGCAAACTAC 
CACGTTGCCA ACGAACCGTC ATGTGCTGGT TATAAAGAGC GCAGCATGAA AGACCTGCCT 
AGCCTACCAT TGGTAAGCAC GTTCTTGGAC TGGTCCATAG AGTATTTCCG CGAGCCGCAG 
ATGAAAGCAT CGGAGGCCGA AGAGCCCGGC AGCCACGAGT ACAACGAACG CCTCTGGCGC 
CGTTCACGCA ACGAGGCCGT TTTGAGAGAG ACTCAGCCCC AGAAGACGCA TGCGCGAACC 
CACAAGTGGA ATGACCAAAT CGGCGTCATT AACAATGGTT CACAACCGGC GAAACTCAGC 
TTTCATCAGT TTGAGAATTA CGTGGCCGTC GCAGATGACG GGAATACAGT GAACGTGTGG 
GATTGGAAAC AGGGTACCCG GAAGAGTCGT TTCTCCAACG GCAACCCAGA AGGCTCCAAG 
ATCTCAGACA TGAAGTTCAT CAACGAAGAC GATCAAGCGC TGTTGATGAC GGGGTCATCA 
GACGGTGTAA TCCGCGTTTA CCGGAATTAC GACAGCAATG AGAACATCGA GCTGGCATCA 
GCTTGGCGCG CTCTGACGCA CATGGTTCCG TCCAACGTCA ACTCGGGCAT GGTGTTCGAC 
TGGCAACAGG TCAAGGGTCA GGTGCTCGTG GCCGGGGATG AGAGGGTCAT TCGCATCTGG 
AATGCCGGTC ATGAAATGTG TACCCATGAA ATTCCAGCAC GATCCGGCTC CTGCGTTACT 
TCGCTCACGT CAGATCAGAT GACAGGCAAC ATCTTTGTCG CCGGATTTGG CGACGGGGCT 
ATCAGAGTCT TCGATACTCG CATGAAGCCG CAGGAAAGCA TGGTTCGGAA ATGGAAGGAC 
GAGAAGAGGC AGTGGGTACG ATCGGTGCAC ATGCAACGCG GTGGACAGAG AGAACTGTTG 
TCGGCCAGCC GCAACGGCAA GATCCGGTTG TGGGATATTC GTATGGACCA GCCGCTCAAA 
TCGTTCCAGA CCACCAAGGA CGTACTCAGG ACGGCAAGCA CCCATGAACA CTTGCCTGTC 
TTTGCTGTTG GAACTTCGGC GCATCTCGTC AAGGTCTTTG ACTTTGATGG CCATGAGCTG 
ACCCGGATCG AGCCCTACTC CAGCTTTCTC CAGGGCCACA AGGGCACGCC TATTTCTGCT 
ACTGCATTCC ATCCTCATCG GATGGTTTTG GGTTGCGCTG CTCGCGGGGA CTATCACGTC 
AACCTGTTTG CTTGTGGGAA CGAGAAGGTT GGCCCCTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_965761.2
CDS	719..5140
Translation	MTAVSLHASHPGSAEFGQRQPSAPTPTSGRYARPGFPSSTATSPSRHTMNNPARQSSVTMSQVHPQRPEDGNQRVATNGTSHPAAHNQLPLRSNIQRRMSDSSPAPLFRRPSSAPDDNNQTSPGTTLHGGQSDDDDHMPVARPAKAPLTRSKSEYGLRLERESPIEQVDEEYHDWGARHGFEDHYQSEEVISQLANSWYMYFTDKRHETTGKPKTPAFEIQDWRMRDRLKTVSAAIAVCLNIGVEPPDQLRTTPGARLEAWQDPTVPPVSKALENIGKALQSQYETLAIRTRYKQYLDPSIEETKRFSVSLRRSAKDERVLFHYNGHGVPKPTASGEIWVFNKNYTQYIPVSLYDLQQWLQAPTIYVWDCSEAGNILTNYHRFVEKHEQEEEEAAQRDPNHEKINFRPYIHLAACNVKENLPTNPDLPADLFTCCLTTPIEMALWFFCLQNPLKTKISPERAKKLPGRLQERRTPLGELNWIFTAITDTIAWTTLPRHLFRKFFRQDLMVAALFRNFLLAQRIMPVYNCHPQSYPELPDTRQHPLWESWDLAVDLALAQLPALERKESEGIEYEYINSSFFAEQLTAFEIYLTRGDALSQKPPDQLPVVLQVLLSQQHRVRALVLLARFLDLGPWAVQLALSIGIFPYVLKLLQSAAQDLKPVMVFIWTRVLAVDISCQQDLIKDSGYNYFAAIMKPQDTLSMVSVGFLDEHKAMCAFILAMLCKDYKPGQLVCNSTDIMTYCLYHIARPEGPLLKQWSCLCISQLWKDLPEGKWRGIRENATSKLSHLSRDPNPEVRAAMAHAMTTFLGIPDLTDEVARIEESVAWTMLELATDGSPIVRKELLVFFSHFVLRYESKFLVAAFEQLSEEKDYLASPPPEDGQDHRMGLHYTRRENRERDGSIKPSAFGVAHDSIFAACWKHINILSVDPHPETQRDATIIVDYVHNALLQSPLTTQTQNLMDEILRRSRKVSRGDMSQRSAAAGTHAAVAQPLPSPGLLKRTASYLFGPLMGTLDPSSTSIPPLSPGLQRSGSRSSRKGPGLDSAPPEQNDQATSPANYHVANEPSCAGYKERSMKDLPSLPLVSTFLDWSIEYFREPQMKASEAEEPGSHEYNERLWRRSRNEAVLRETQPQKTHARTHKWNDQIGVINNGSQPAKLSFHQFENYVAVADDGNTVNVWDWKQGTRKSRFSNGNPEGSKISDMKFINEDDQALLMTGSSDGVIRVYRNYDSNENIELASAWRALTHMVPSNVNSGMVFDWQQVKGQVLVAGDERVIRIWNAGHEMCTHEIPARSGSCVTSLTSDQMTGNIFVAGFGDGAIRVFDTRMKPQESMVRKWKDEKRQWVRSVHMQRGGQRELLSASRNGKIRLWDIRMDQPLKSFQTTKDVLRTASTHEHLPVFAVGTSAHLVKVFDFDGHELTRIEPYSSFLQGHKGTPISATAFHPHRMVLGCAARGDYHVNLFACGNEKVGPF

Target ORF information:

RefSeq Version	XM_960668.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A TORC1 growth control complex subunit Kog1 (NCU00621), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_960668.2

ORF Insert Sequence:

ATGACTGCTG TTTCTCTTCA CGCCTCCCAC CCCGGCTCCG CAGAATTTGG CCAGAGACAG 
CCCTCTGCTC CCACTCCCAC CTCTGGCCGT TACGCGAGGC CCGGCTTTCC GTCATCAACT 
GCTACATCAC CATCCCGCCA CACAATGAAC AACCCAGCTC GTCAAAGTTC AGTCACAATG 
TCACAAGTCC ATCCTCAGAG GCCAGAAGAC GGCAACCAGC GCGTCGCGAC AAACGGGACC 
TCTCATCCTG CTGCCCATAA CCAGCTGCCA CTTCGATCCA ACATACAGAG ACGCATGTCC 
GATTCCTCAC CAGCGCCCTT GTTTCGACGC CCCTCCAGCG CCCCCGACGA CAATAACCAA 
ACATCTCCTG GAACCACGTT GCATGGCGGG CAATCTGATG ACGACGACCA TATGCCTGTC 
GCCCGGCCGG CCAAGGCGCC ATTAACGCGG TCTAAGAGCG AATACGGACT GCGGTTGGAG 
AGGGAAAGCC CCATCGAACA AGTTGACGAA GAGTACCATG ATTGGGGTGC CAGGCATGGG 
TTCGAGGACC ATTATCAGTC TGAAGAAGTC ATTTCTCAGC TTGCAAATAG CTGGTACATG 
TACTTTACCG ATAAGCGACA TGAGACAACA GGGAAGCCAA AGACACCCGC TTTCGAGATC 
CAAGACTGGC GTATGCGCGA TCGCCTAAAA ACAGTTTCTG CTGCGATAGC AGTATGCTTG 
AACATAGGAG TGGAACCGCC AGATCAACTT CGCACCACCC CGGGTGCCCG GCTTGAAGCT 
TGGCAAGATC CGACGGTGCC TCCTGTTTCC AAGGCACTGG AGAATATTGG GAAAGCTTTG 
CAATCTCAAT ATGAGACCCT TGCTATTCGG ACGCGCTACA AGCAGTATTT AGACCCGTCC 
ATCGAAGAAA CCAAGCGGTT CAGTGTCTCC CTCCGAAGAA GTGCCAAGGA CGAGCGTGTG 
CTCTTCCATT ACAATGGGCA TGGAGTGCCC AAGCCAACAG CCTCGGGAGA GATCTGGGTT 
TTCAACAAGA ACTACACGCA GTATATCCCC GTCTCTCTCT ACGACCTTCA GCAATGGTTG 
CAGGCGCCCA CGATATATGT GTGGGATTGC TCCGAGGCTG GCAATATCCT CACCAATTAC 
CACCGTTTTG TTGAGAAGCA CGAGCAAGAA GAAGAGGAGG CGGCACAACG GGACCCAAAC 
CACGAAAAGA TTAACTTCCG TCCCTACATC CATCTGGCAG CCTGCAACGT CAAGGAGAAT 
CTGCCAACCA ACCCCGACCT GCCTGCTGAT CTCTTCACTT GCTGCTTGAC TACCCCTATA 
GAGATGGCAC TCTGGTTCTT CTGTCTGCAA AATCCGCTAA AAACCAAAAT ATCTCCTGAG 
CGAGCCAAGA AGCTGCCAGG GCGGCTTCAG GAGCGCCGGA CGCCTCTCGG AGAGCTCAAC 
TGGATTTTCA CTGCCATCAC AGATACCATT GCATGGACCA CGCTACCACG CCATCTCTTC 
CGCAAATTTT TTAGGCAAGA TTTGATGGTT GCCGCTCTTT TCCGAAACTT TCTCCTCGCA 
CAGCGTATCA TGCCTGTGTA CAACTGCCAT CCCCAGTCGT ATCCAGAGTT GCCAGATACA 
CGTCAGCATC CCCTTTGGGA GAGCTGGGAC TTGGCCGTCG ATCTGGCTCT TGCCCAGTTG 
CCGGCCCTCG AGAGAAAGGA GAGTGAAGGG ATAGAGTATG AGTACATCAA CTCGTCATTC 
TTCGCGGAGC AGCTCACAGC ATTCGAGATC TACTTGACTC GAGGGGATGC TTTGTCCCAG 
AAGCCACCTG ACCAGCTTCC AGTGGTTCTG CAAGTGTTGC TAAGTCAGCA GCATCGAGTA 
CGAGCTCTGG TCCTACTAGC AAGGTTCTTG GACCTTGGCC CTTGGGCAGT ACAACTGGCG 
CTCAGCATTG GCATCTTCCC GTACGTCTTG AAGCTCCTCC AGTCGGCGGC ACAAGACCTA 
AAGCCAGTCA TGGTGTTCAT CTGGACTCGC GTTCTTGCGG TCGATATCTC ATGCCAGCAA 
GACCTCATCA AAGATAGCGG GTACAATTAC TTCGCGGCCA TAATGAAGCC GCAAGATACC 
CTCTCCATGG TTAGCGTCGG GTTCTTGGAT GAGCACAAGG CGATGTGTGC TTTCATCTTG 
GCTATGCTGT GCAAAGACTA TAAACCTGGA CAGCTTGTGT GTAACTCAAC TGACATTATG 
ACCTATTGCC TGTACCATAT TGCACGCCCA GAAGGCCCCC TCCTGAAGCA ATGGTCATGC 
CTCTGCATCA GCCAGCTCTG GAAGGACCTT CCTGAAGGTA AATGGAGGGG CATTCGGGAG 
AACGCCACTT CGAAACTCTC CCACCTTAGT AGGGACCCCA ACCCTGAAGT ACGGGCAGCT 
ATGGCGCATG CTATGACCAC GTTCCTAGGA ATCCCGGATC TCACGGACGA AGTTGCGAGG 
ATCGAGGAAA GCGTTGCCTG GACCATGCTT GAACTGGCTA CAGACGGCAG CCCGATTGTC 
CGAAAAGAGC TTCTAGTCTT CTTCTCTCAT TTCGTTCTGC GTTATGAGAG CAAGTTCCTG 
GTTGCCGCCT TCGAGCAACT ATCCGAGGAG AAGGATTATC TTGCTTCGCC TCCGCCAGAG 
GATGGCCAAG ATCACAGAAT GGGGTTACAC TATACTCGCC GAGAAAACCG GGAGAGAGAT 
GGCAGTATCA AACCATCAGC ATTTGGTGTA GCGCATGATT CGATCTTCGC TGCCTGCTGG 
AAACACATCA ACATCTTGAG CGTGGATCCA CATCCCGAAA CCCAAAGAGA TGCCACCATC 
ATCGTGGATT ATGTTCACAA TGCACTTCTT CAATCACCAC TTACAACGCA AACGCAAAAT 
CTCATGGACG AGATCCTCCG GCGCTCTAGA AAAGTGTCGA GGGGTGATAT GAGCCAGAGA 
AGCGCCGCTG CCGGCACTCA CGCTGCAGTC GCACAGCCAT TGCCTTCGCC AGGCCTTCTC 
AAGAGGACTG CAAGTTACCT GTTTGGCCCC CTCATGGGGA CCCTGGACCC TTCTTCAACC 
TCGATTCCGC CACTGTCCCC TGGGCTACAA AGAAGTGGAT CTCGAAGCAG CCGAAAAGGT 
CCGGGCCTGG ATTCTGCGCC ACCTGAGCAG AATGATCAGG CTACTTCGCC AGCAAACTAC 
CACGTTGCCA ACGAACCGTC ATGTGCTGGT TATAAAGAGC GCAGCATGAA AGACCTGCCT 
AGCCTACCAT TGGTAAGCAC GTTCTTGGAC TGGTCCATAG AGTATTTCCG CGAGCCGCAG 
ATGAAAGCAT CGGAGGCCGA AGAGCCCGGC AGCCACGAGT ACAACGAACG CCTCTGGCGC 
CGTTCACGCA ACGAGGCCGT TTTGAGAGAG ACTCAGCCCC AGAAGACGCA TGCGCGAACC 
CACAAGTGGA ATGACCAAAT CGGCGTCATT AACAATGGTT CACAACCGGC GAAACTCAGC 
TTTCATCAGT TTGAGAATTA CGTGGCCGTC GCAGATGACG GGAATACAGT GAACGTGTGG 
GATTGGAAAC AGGGTACCCG GAAGAGTCGT TTCTCCAACG GCAACCCAGA AGGCTCCAAG 
ATCTCAGACA TGAAGTTCAT CAACGAAGAC GATCAAGCGC TGTTGATGAC GGGGTCATCA 
GACGGTGTAA TCCGCGTTTA CCGGAATTAC GACAGCAATG AGAACATCGA GCTGGCATCA 
GCTTGGCGCG CTCTGACGCA CATGGTTCCG TCCAACGTCA ACTCGGGCAT GGTGTTCGAC 
TGGCAACAGG TCAAGGGTCA GGTGCTCGTG GCCGGGGATG AGAGGGTCAT TCGCATCTGG 
AATGCCGGTC ATGAAATGTG TACCCATGAA ATTCCAGCAC GATCCGGCTC CTGCGTTACT 
TCGCTCACGT CAGATCAGAT GACAGGCAAC ATCTTTGTCG CCGGATTTGG CGACGGGGCT 
ATCAGAGTCT TCGATACTCG CATGAAGCCG CAGGAAAGCA TGGTTCGGAA ATGGAAGGAC 
GAGAAGAGGC AGTGGGTACG ATCGGTGCAC ATGCAACGCG GTGGACAGAG AGAACTGTTG 
TCGGCCAGCC GCAACGGCAA GATCCGGTTG TGGGATATTC GTATGGACCA GCCGCTCAAA 
TCGTTCCAGA CCACCAAGGA CGTACTCAGG ACGGCAAGCA CCCATGAACA CTTGCCTGTC 
TTTGCTGTTG GAACTTCGGC GCATCTCGTC AAGGTCTTTG ACTTTGATGG CCATGAGCTG 
ACCCGGATCG AGCCCTACTC CAGCTTTCTC CAGGGCCACA AGGGCACGCC TATTTCTGCT 
ACTGCATTCC ATCCTCATCG GATGGTTTTG GGTTGCGCTG CTCGCGGGGA CTATCACGTC 
AACCTGTTTG CTTGTGGGAA CGAGAAGGTT GGCCCCTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU00621 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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