rptor gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012971554.2	XP_012827008.1	regulatory-associated protein of mTOR isoform X2
XM_012971554.3	XP_012827008.1	regulatory-associated protein of mTOR isoform X2
XM_012971555.2	XP_012827009.1	regulatory-associated protein of mTOR isoform X3
XM_012971553.3	XP_012827007.1	regulatory-associated protein of mTOR isoform X1
XM_012971555.3	XP_012827009.1	regulatory-associated protein of mTOR isoform X3
XM_012971553.2	XP_012827007.1	regulatory-associated protein of mTOR isoform X1
XM_012971556.3	XP_012827010.1	regulatory-associated protein of mTOR isoform X4
XM_012971556.2	XP_012827010.1	regulatory-associated protein of mTOR isoform X4
XM_012971557.2	XP_012827011.1	regulatory-associated protein of mTOR isoform X5
NM_001130371.1	NP_001123843.1	regulatory-associated protein of mTOR

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa49975	XM_012971553.3 Latest version!	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa49976	XM_012971554.3 Latest version!	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa49977	XM_012971555.3 Latest version!	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa49978	XM_012971556.3 Latest version!	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa24060	NM_001130371.1 Latest version!	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa32468	XM_012971557.2 Latest version!	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa32464	XM_012971553.2 Latest version!	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32465	XM_012971554.2 Latest version!	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32466	XM_012971555.2 Latest version!	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32467	XM_012971556.2 Latest version!	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

rptor ( XM_012971553.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827007.1 Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa49975
Clone ID Related Accession (Same CDS sequence)	XM_012971553.3
Accession Version	XM_012971553.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulatory-associated protein of mTOR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971553.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTGGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG GAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACG CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTC TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTTTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TTGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGAGTAA GACTGCTGGG AGTGCCATTA CATTTTCTCC TGGGAATCTA 
AGTACAAGTA GTAGCGCAAG CAGTACGTTA GGAAGCCCAG ATAATGAAGA GTACATCTTG 
TCCTTTGAGA CCATTGATAA GATGAGGCGT GTCAGTTCTT ACTCCTCTCT CAATTCCCTC 
ATAGGTGTCT CGTTCAATAG TGTCTACACT CAAATTTGGA GAGTGCTTCT CCACCTTGCT 
GCTGACCCAT ACCCTGAAGT CTCGGACCTT GCAATGAAAG TGCTAAACAG CATTGCTCAT 
AAGGCTACAG TGAATGCTCG CCCACAGCGG ATCCTCGATG CCTCCTCACT CACGCAGTCT 
GCTCCCGCCA GTCCCACCAA CAAAGGGATG CACATTCACC AAGTGGGTGG TTCCCCTCCG 
ACTCCCAGTG CCAGCAGTTC CAGTCTCACC AACGATGTAA CGAAACCACC TGTTAGTAGG 
GATATTCCCT CTAATCGACC TGCCAATGCT GGGGGCAATG TTGGGGTGCA GTACACGCCT 
CATTCCCACC AGTTTCCTCG TACAAGGAAA ATGTTCGACA AAGGACCAGA CCAGCCTGGA 
GATGATGGAG ATGAAGCCAT TGTACACAAG AACTTCACTG TAGGATCTTT TCAGACAGGC 
TTTTGTGATT GGAGTGCCAA ATATTTTGCC CAGCCAGTCA TGAAGATGCC AGAGGAACAT 
GACATGGAGA GTCAGGTACG TAAAGAACGA GAGTGGCGAT TTCTACGAAA TACAAGGGTA 
CGGAAACAGG CCCAGCGCCT TATACAGAAA GGAATCTCAA GACTCGATGA CCAGATCTTT 
ATCAACCGGA ATCCAGGAGT TCCTTCTGTT GTGAAGTTCC ATCCTTTTAC CTCAAGCATT 
GCTGTAGCAG ACAAGGACAG CATTTGTTTC TGGGACTGGG AGAAAGGAGA GAAGCTGGAT 
TACTTTCACA ATGGTAACCC TCGTTATACT CGTATAACAG CAGTCGAATA TCTGAATGGC 
CAAGATTGCT CTTTGCTGCT TACTGCTACA GATGATGGAG CCATCCGCGT GTGGAAGAAC 
TTTGCTGACT TAGAGAAAAA TCCAGAGATG GTGACAGCTT GGCAGGGACT GTCCGATATG 
CTGCCGACCA CACGAGGCCT GGCTCGAAGA GTTTCCATTT ATCTTGACAG GAGTAAACAG 
GGGGGGGCTG GCATGGTGGT GGATTGGGAG CAAGAAACAG GTCTACTCAT CACATCTGGA 
GACGTGCGAA TTATCCGGCT ATGGGACACA GACAGAGAGA CAAAAGTGCA GGATATACCC 
ACTGGAGCAG ACAGTTGTGT GACCAGCCTG TCATGTGACT CTCATCGTTC CCTTATTGTG 
GCTGGACTCG GAGATGGTTC AGTGCGAGTG TTTGATCGCA GGATGGCACT GAATGAATGC 
CGTGTAATGA CGTACCGAGA GCACTCAGCC TGGGTAGTAA AGACTTCTCT CCAAAAGCAG 
TCCGATGGGA ATATTGTGAG CGTCAGTGTA AATGGAGATA TCCGTTACTT CGACCCTCGT 
ATGCCAGAAT GTTTAAACTC ACTGCAGATT GTGAAGGGTC TTACTGCGTT GGATATCCAC 
CCACAGGCTA ACCTGTTTGC ATGTGGTTCA ATGAACCAGT TCATCGCTAT TTACAATGGA 
AATGGAGAGC TGATCAGCAA CATAAAGTAC TACGATGGAT TTATGGGACA AAGAATAGGA 
GCCATTAGCT GCCTGGCCTT TCATCCTTAC TGGCCCCACC TGGCCGTTGG CAGTAATGAC 
TATTATATGT CTATTTACTC AGTGGAGAAG CGAGCGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827007.1
CDS	116..4177
Translation	MELHTPALGLVGEDEETDLSDWNLPSAFMKKRHCEKIEGCKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLAMGPQKALETIGANLQKQYETWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPSHPLAQMPLPPSMKNCIQLAACDAAELLPMNPDLPADLFTSCLTTPIKIALRWFCMQKSVRLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCSPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTSFILAVIVNNYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDAARWCGVRDSAHEKLYSLLSDPMPEVRSSAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPMVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPASTEGGSLTPIRDSPGTPRLRSVSSYGNIRAVTTPRNLNKSLQNLSLSEESKTAGSAITFSPGNLSTSSSASSTLGSPDNEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDLAMKVLNSIAHKATVNARPQRILDASSLTQSAPASPTNKGMHIHQVGGSPPTPSASSSSLTNDVTKPPVSRDIPSNRPANAGGNVGVQYTPHSHQFPRTRKMFDKGPDQPGDDGDEAIVHKNFTVGSFQTGFCDWSAKYFAQPVMKMPEEHDMESQVRKEREWRFLRNTRVRKQAQRLIQKGISRLDDQIFINRNPGVPSVVKFHPFTSSIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRITAVEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGLARRVSIYLDRSKQGGAGMVVDWEQETGLLITSGDVRIIRLWDTDRETKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSVRVFDRRMALNECRVMTYREHSAWVVKTSLQKQSDGNIVSVSVNGDIRYFDPRMPECLNSLQIVKGLTALDIHPQANLFACGSMNQFIAIYNGNGELISNIKYYDGFMGQRIGAISCLAFHPYWPHLAVGSNDYYMSIYSVEKRAR

Target ORF information:

RefSeq Version	XM_012971553.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971553.3

ORF Insert Sequence:

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTGGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG GAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACG CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTC TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTTTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TTGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGAGTAA GACTGCTGGG AGTGCCATTA CATTTTCTCC TGGGAATCTA 
AGTACAAGTA GTAGCGCAAG CAGTACGTTA GGAAGCCCAG ATAATGAAGA GTACATCTTG 
TCCTTTGAGA CCATTGATAA GATGAGGCGT GTCAGTTCTT ACTCCTCTCT CAATTCCCTC 
ATAGGTGTCT CGTTCAATAG TGTCTACACT CAAATTTGGA GAGTGCTTCT CCACCTTGCT 
GCTGACCCAT ACCCTGAAGT CTCGGACCTT GCAATGAAAG TGCTAAACAG CATTGCTCAT 
AAGGCTACAG TGAATGCTCG CCCACAGCGG ATCCTCGATG CCTCCTCACT CACGCAGTCT 
GCTCCCGCCA GTCCCACCAA CAAAGGGATG CACATTCACC AAGTGGGTGG TTCCCCTCCG 
ACTCCCAGTG CCAGCAGTTC CAGTCTCACC AACGATGTAA CGAAACCACC TGTTAGTAGG 
GATATTCCCT CTAATCGACC TGCCAATGCT GGGGGCAATG TTGGGGTGCA GTACACGCCT 
CATTCCCACC AGTTTCCTCG TACAAGGAAA ATGTTCGACA AAGGACCAGA CCAGCCTGGA 
GATGATGGAG ATGAAGCCAT TGTACACAAG AACTTCACTG TAGGATCTTT TCAGACAGGC 
TTTTGTGATT GGAGTGCCAA ATATTTTGCC CAGCCAGTCA TGAAGATGCC AGAGGAACAT 
GACATGGAGA GTCAGGTACG TAAAGAACGA GAGTGGCGAT TTCTACGAAA TACAAGGGTA 
CGGAAACAGG CCCAGCGCCT TATACAGAAA GGAATCTCAA GACTCGATGA CCAGATCTTT 
ATCAACCGGA ATCCAGGAGT TCCTTCTGTT GTGAAGTTCC ATCCTTTTAC CTCAAGCATT 
GCTGTAGCAG ACAAGGACAG CATTTGTTTC TGGGACTGGG AGAAAGGAGA GAAGCTGGAT 
TACTTTCACA ATGGTAACCC TCGTTATACT CGTATAACAG CAGTCGAATA TCTGAATGGC 
CAAGATTGCT CTTTGCTGCT TACTGCTACA GATGATGGAG CCATCCGCGT GTGGAAGAAC 
TTTGCTGACT TAGAGAAAAA TCCAGAGATG GTGACAGCTT GGCAGGGACT GTCCGATATG 
CTGCCGACCA CACGAGGCCT GGCTCGAAGA GTTTCCATTT ATCTTGACAG GAGTAAACAG 
GGGGGGGCTG GCATGGTGGT GGATTGGGAG CAAGAAACAG GTCTACTCAT CACATCTGGA 
GACGTGCGAA TTATCCGGCT ATGGGACACA GACAGAGAGA CAAAAGTGCA GGATATACCC 
ACTGGAGCAG ACAGTTGTGT GACCAGCCTG TCATGTGACT CTCATCGTTC CCTTATTGTG 
GCTGGACTCG GAGATGGTTC AGTGCGAGTG TTTGATCGCA GGATGGCACT GAATGAATGC 
CGTGTAATGA CGTACCGAGA GCACTCAGCC TGGGTAGTAA AGACTTCTCT CCAAAAGCAG 
TCCGATGGGA ATATTGTGAG CGTCAGTGTA AATGGAGATA TCCGTTACTT CGACCCTCGT 
ATGCCAGAAT GTTTAAACTC ACTGCAGATT GTGAAGGGTC TTACTGCGTT GGATATCCAC 
CCACAGGCTA ACCTGTTTGC ATGTGGTTCA ATGAACCAGT TCATCGCTAT TTACAATGGA 
AATGGAGAGC TGATCAGCAA CATAAAGTAC TACGATGGAT TTATGGGACA AAGAATAGGA 
GCCATTAGCT GCCTGGCCTT TCATCCTTAC TGGCCCCACC TGGCCGTTGG CAGTAATGAC 
TATTATATGT CTATTTACTC AGTGGAGAAG CGAGCGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rptor ( XM_012971554.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827008.1 Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa49976
Clone ID Related Accession (Same CDS sequence)	XM_012971554.3
Accession Version	XM_012971554.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulatory-associated protein of mTOR isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971554.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTGGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG GAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACG CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTC TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTTTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TTGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGACTGG GAGTGCCATT ACATTTTCTC CTGGGAATCT AAGTACAAGT 
AGTAGCGCAA GCAGTACGTT AGGAAGCCCA GATAATGAAG AGTACATCTT GTCCTTTGAG 
ACCATTGATA AGATGAGGCG TGTCAGTTCT TACTCCTCTC TCAATTCCCT CATAGGTGTC 
TCGTTCAATA GTGTCTACAC TCAAATTTGG AGAGTGCTTC TCCACCTTGC TGCTGACCCA 
TACCCTGAAG TCTCGGACCT TGCAATGAAA GTGCTAAACA GCATTGCTCA TAAGGCTACA 
GTGAATGCTC GCCCACAGCG GATCCTCGAT GCCTCCTCAC TCACGCAGTC TGCTCCCGCC 
AGTCCCACCA ACAAAGGGAT GCACATTCAC CAAGTGGGTG GTTCCCCTCC GACTCCCAGT 
GCCAGCAGTT CCAGTCTCAC CAACGATGTA ACGAAACCAC CTGTTAGTAG GGATATTCCC 
TCTAATCGAC CTGCCAATGC TGGGGGCAAT GTTGGGGTGC AGTACACGCC TCATTCCCAC 
CAGTTTCCTC GTACAAGGAA AATGTTCGAC AAAGGACCAG ACCAGCCTGG AGATGATGGA 
GATGAAGCCA TTGTACACAA GAACTTCACT GTAGGATCTT TTCAGACAGG CTTTTGTGAT 
TGGAGTGCCA AATATTTTGC CCAGCCAGTC ATGAAGATGC CAGAGGAACA TGACATGGAG 
AGTCAGGTAC GTAAAGAACG AGAGTGGCGA TTTCTACGAA ATACAAGGGT ACGGAAACAG 
GCCCAGCGCC TTATACAGAA AGGAATCTCA AGACTCGATG ACCAGATCTT TATCAACCGG 
AATCCAGGAG TTCCTTCTGT TGTGAAGTTC CATCCTTTTA CCTCAAGCAT TGCTGTAGCA 
GACAAGGACA GCATTTGTTT CTGGGACTGG GAGAAAGGAG AGAAGCTGGA TTACTTTCAC 
AATGGTAACC CTCGTTATAC TCGTATAACA GCAGTCGAAT ATCTGAATGG CCAAGATTGC 
TCTTTGCTGC TTACTGCTAC AGATGATGGA GCCATCCGCG TGTGGAAGAA CTTTGCTGAC 
TTAGAGAAAA ATCCAGAGAT GGTGACAGCT TGGCAGGGAC TGTCCGATAT GCTGCCGACC 
ACACGAGGCC TGGCTCGAAG AGTTTCCATT TATCTTGACA GGAGTAAACA GGGGGGGGCT 
GGCATGGTGG TGGATTGGGA GCAAGAAACA GGTCTACTCA TCACATCTGG AGACGTGCGA 
ATTATCCGGC TATGGGACAC AGACAGAGAG ACAAAAGTGC AGGATATACC CACTGGAGCA 
GACAGTTGTG TGACCAGCCT GTCATGTGAC TCTCATCGTT CCCTTATTGT GGCTGGACTC 
GGAGATGGTT CAGTGCGAGT GTTTGATCGC AGGATGGCAC TGAATGAATG CCGTGTAATG 
ACGTACCGAG AGCACTCAGC CTGGGTAGTA AAGACTTCTC TCCAAAAGCA GTCCGATGGG 
AATATTGTGA GCGTCAGTGT AAATGGAGAT ATCCGTTACT TCGACCCTCG TATGCCAGAA 
TGTTTAAACT CACTGCAGAT TGTGAAGGGT CTTACTGCGT TGGATATCCA CCCACAGGCT 
AACCTGTTTG CATGTGGTTC AATGAACCAG TTCATCGCTA TTTACAATGG AAATGGAGAG 
CTGATCAGCA ACATAAAGTA CTACGATGGA TTTATGGGAC AAAGAATAGG AGCCATTAGC 
TGCCTGGCCT TTCATCCTTA CTGGCCCCAC CTGGCCGTTG GCAGTAATGA CTATTATATG 
TCTATTTACT CAGTGGAGAA GCGAGCGAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827008.1
CDS	116..4168
Translation	MELHTPALGLVGEDEETDLSDWNLPSAFMKKRHCEKIEGCKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLAMGPQKALETIGANLQKQYETWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPSHPLAQMPLPPSMKNCIQLAACDAAELLPMNPDLPADLFTSCLTTPIKIALRWFCMQKSVRLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCSPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTSFILAVIVNNYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDAARWCGVRDSAHEKLYSLLSDPMPEVRSSAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPMVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPASTEGGSLTPIRDSPGTPRLRSVSSYGNIRAVTTPRNLNKSLQNLSLSEETGSAITFSPGNLSTSSSASSTLGSPDNEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDLAMKVLNSIAHKATVNARPQRILDASSLTQSAPASPTNKGMHIHQVGGSPPTPSASSSSLTNDVTKPPVSRDIPSNRPANAGGNVGVQYTPHSHQFPRTRKMFDKGPDQPGDDGDEAIVHKNFTVGSFQTGFCDWSAKYFAQPVMKMPEEHDMESQVRKEREWRFLRNTRVRKQAQRLIQKGISRLDDQIFINRNPGVPSVVKFHPFTSSIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRITAVEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGLARRVSIYLDRSKQGGAGMVVDWEQETGLLITSGDVRIIRLWDTDRETKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSVRVFDRRMALNECRVMTYREHSAWVVKTSLQKQSDGNIVSVSVNGDIRYFDPRMPECLNSLQIVKGLTALDIHPQANLFACGSMNQFIAIYNGNGELISNIKYYDGFMGQRIGAISCLAFHPYWPHLAVGSNDYYMSIYSVEKRAR

Target ORF information:

RefSeq Version	XM_012971554.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971554.3

ORF Insert Sequence:

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTGGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG GAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACG CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTC TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTTTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TTGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGACTGG GAGTGCCATT ACATTTTCTC CTGGGAATCT AAGTACAAGT 
AGTAGCGCAA GCAGTACGTT AGGAAGCCCA GATAATGAAG AGTACATCTT GTCCTTTGAG 
ACCATTGATA AGATGAGGCG TGTCAGTTCT TACTCCTCTC TCAATTCCCT CATAGGTGTC 
TCGTTCAATA GTGTCTACAC TCAAATTTGG AGAGTGCTTC TCCACCTTGC TGCTGACCCA 
TACCCTGAAG TCTCGGACCT TGCAATGAAA GTGCTAAACA GCATTGCTCA TAAGGCTACA 
GTGAATGCTC GCCCACAGCG GATCCTCGAT GCCTCCTCAC TCACGCAGTC TGCTCCCGCC 
AGTCCCACCA ACAAAGGGAT GCACATTCAC CAAGTGGGTG GTTCCCCTCC GACTCCCAGT 
GCCAGCAGTT CCAGTCTCAC CAACGATGTA ACGAAACCAC CTGTTAGTAG GGATATTCCC 
TCTAATCGAC CTGCCAATGC TGGGGGCAAT GTTGGGGTGC AGTACACGCC TCATTCCCAC 
CAGTTTCCTC GTACAAGGAA AATGTTCGAC AAAGGACCAG ACCAGCCTGG AGATGATGGA 
GATGAAGCCA TTGTACACAA GAACTTCACT GTAGGATCTT TTCAGACAGG CTTTTGTGAT 
TGGAGTGCCA AATATTTTGC CCAGCCAGTC ATGAAGATGC CAGAGGAACA TGACATGGAG 
AGTCAGGTAC GTAAAGAACG AGAGTGGCGA TTTCTACGAA ATACAAGGGT ACGGAAACAG 
GCCCAGCGCC TTATACAGAA AGGAATCTCA AGACTCGATG ACCAGATCTT TATCAACCGG 
AATCCAGGAG TTCCTTCTGT TGTGAAGTTC CATCCTTTTA CCTCAAGCAT TGCTGTAGCA 
GACAAGGACA GCATTTGTTT CTGGGACTGG GAGAAAGGAG AGAAGCTGGA TTACTTTCAC 
AATGGTAACC CTCGTTATAC TCGTATAACA GCAGTCGAAT ATCTGAATGG CCAAGATTGC 
TCTTTGCTGC TTACTGCTAC AGATGATGGA GCCATCCGCG TGTGGAAGAA CTTTGCTGAC 
TTAGAGAAAA ATCCAGAGAT GGTGACAGCT TGGCAGGGAC TGTCCGATAT GCTGCCGACC 
ACACGAGGCC TGGCTCGAAG AGTTTCCATT TATCTTGACA GGAGTAAACA GGGGGGGGCT 
GGCATGGTGG TGGATTGGGA GCAAGAAACA GGTCTACTCA TCACATCTGG AGACGTGCGA 
ATTATCCGGC TATGGGACAC AGACAGAGAG ACAAAAGTGC AGGATATACC CACTGGAGCA 
GACAGTTGTG TGACCAGCCT GTCATGTGAC TCTCATCGTT CCCTTATTGT GGCTGGACTC 
GGAGATGGTT CAGTGCGAGT GTTTGATCGC AGGATGGCAC TGAATGAATG CCGTGTAATG 
ACGTACCGAG AGCACTCAGC CTGGGTAGTA AAGACTTCTC TCCAAAAGCA GTCCGATGGG 
AATATTGTGA GCGTCAGTGT AAATGGAGAT ATCCGTTACT TCGACCCTCG TATGCCAGAA 
TGTTTAAACT CACTGCAGAT TGTGAAGGGT CTTACTGCGT TGGATATCCA CCCACAGGCT 
AACCTGTTTG CATGTGGTTC AATGAACCAG TTCATCGCTA TTTACAATGG AAATGGAGAG 
CTGATCAGCA ACATAAAGTA CTACGATGGA TTTATGGGAC AAAGAATAGG AGCCATTAGC 
TGCCTGGCCT TTCATCCTTA CTGGCCCCAC CTGGCCGTTG GCAGTAATGA CTATTATATG 
TCTATTTACT CAGTGGAGAA GCGAGCGAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rptor ( XM_012971555.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827009.1 Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X3, mRNA.

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CloneID	OXa49977
Clone ID Related Accession (Same CDS sequence)	XM_012971555.3
Accession Version	XM_012971555.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulatory-associated protein of mTOR isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971555.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTGGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG GAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACG CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTC TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTTTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TTGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGAGTAA GACTGCTGGG AGTGCCATTA CATTTTCTCC TGGGAATCTA 
AGTACAAGTA GTAGCGCAAG CAGTACGTTA GGAAGCCCAG ATAATGAAGA GTACATCTTG 
TCCTTTGAGA CCATTGATAA GATGAGGCGT GTCAGTTCTT ACTCCTCTCT CAATTCCCTC 
ATAGGTGTCT CGTTCAATAG TGTCTACACT CAAATTTGGA GAGTGCTTCT CCACCTTGCT 
GCTGACCCAT ACCCTGAAGT CTCGGACCTT GCAATGAAAG TGCTAAACAG CATTGCTCAT 
AAGGCTACAG TGAATGCTCG CCCACAGCGG ATCCTCGATG CCTCCTCACT CACGCAGTCT 
GCTCCCGCCA GTCCCACCAA CAAAGGGATG CACATTCACC AAGTGGGTGG TTCCCCTCCG 
ACTCCCAGTG CCAGCAGTTC CAGTCTCACC AACGATGTAA CGAAACCACC TGTTAGTAGG 
GATATTCCCT CTAATCGACC TGCCAATGCT GGGGGCAATG TTGGGGTGCA GTACACGCCT 
CATTCCCACC AGTTTCCTCG TACAAGGAAA ATGTTCGACA AAGGACCAGA CCAGCCTGGA 
GATGATGGAG ATGAAGCCAT TGTACACAAG AACTTCACTG TAGGATCTTT TCAGACAGGC 
TTTTGTGATT GGAGTGCCAA ATATTTTGCC CAGCCAGTCA TGAAGATGCC AGAGGAACAT 
GACATGGAGA GTCAGGTACG TAAAGAACGA GAGTGGCGAT TTCTACGAAA TACAAGGGTA 
CGGAAACAGG CCCAGCGCCT TATACAGAAA GGAATCTCAA GACTCGATGA CCAGATCTTT 
ATCAACCGGA ATCCAGGAGT TCCTTCTGTT GTGAAGTTCC ATCCTTTTAC CTCAAGCATT 
GCTGTAGCAG ACAAGGACAG CATTTGTTTC TGGGACTGGG AGAAAGGAGA GAAGCTGGAT 
TACTTTCACA ATGGTAACCC TCGTTATACT CGTATAACAG CAGTCGAATA TCTGAATGGC 
CAAGATTGCT CTTTGCTGCT TACTGCTACA GATGATGGAG CCATCCGCGT GTGGAAGAAC 
TTTGCTGACT TAGAGAAAAA TCCAGAGATG GTGACAGCTT GGCAGGGACT GTCCGATATG 
CTGCCGACCA CACGAGGGGC TGGCATGGTG GTGGATTGGG AGCAAGAAAC AGGTCTACTC 
ATCACATCTG GAGACGTGCG AATTATCCGG CTATGGGACA CAGACAGAGA GACAAAAGTG 
CAGGATATAC CCACTGGAGC AGACAGTTGT GTGACCAGCC TGTCATGTGA CTCTCATCGT 
TCCCTTATTG TGGCTGGACT CGGAGATGGT TCAGTGCGAG TGTTTGATCG CAGGATGGCA 
CTGAATGAAT GCCGTGTAAT GACGTACCGA GAGCACTCAG CCTGGGTAGT AAAGACTTCT 
CTCCAAAAGC AGTCCGATGG GAATATTGTG AGCGTCAGTG TAAATGGAGA TATCCGTTAC 
TTCGACCCTC GTATGCCAGA ATGTTTAAAC TCACTGCAGA TTGTGAAGGG TCTTACTGCG 
TTGGATATCC ACCCACAGGC TAACCTGTTT GCATGTGGTT CAATGAACCA GTTCATCGCT 
ATTTACAATG GAAATGGAGA GCTGATCAGC AACATAAAGT ACTACGATGG ATTTATGGGA 
CAAAGAATAG GAGCCATTAG CTGCCTGGCC TTTCATCCTT ACTGGCCCCA CCTGGCCGTT 
GGCAGTAATG ACTATTATAT GTCTATTTAC TCAGTGGAGA AGCGAGCGAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827009.1
CDS	116..4129
Translation	MELHTPALGLVGEDEETDLSDWNLPSAFMKKRHCEKIEGCKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLAMGPQKALETIGANLQKQYETWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPSHPLAQMPLPPSMKNCIQLAACDAAELLPMNPDLPADLFTSCLTTPIKIALRWFCMQKSVRLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCSPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTSFILAVIVNNYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDAARWCGVRDSAHEKLYSLLSDPMPEVRSSAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPMVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPASTEGGSLTPIRDSPGTPRLRSVSSYGNIRAVTTPRNLNKSLQNLSLSEESKTAGSAITFSPGNLSTSSSASSTLGSPDNEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDLAMKVLNSIAHKATVNARPQRILDASSLTQSAPASPTNKGMHIHQVGGSPPTPSASSSSLTNDVTKPPVSRDIPSNRPANAGGNVGVQYTPHSHQFPRTRKMFDKGPDQPGDDGDEAIVHKNFTVGSFQTGFCDWSAKYFAQPVMKMPEEHDMESQVRKEREWRFLRNTRVRKQAQRLIQKGISRLDDQIFINRNPGVPSVVKFHPFTSSIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRITAVEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLITSGDVRIIRLWDTDRETKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSVRVFDRRMALNECRVMTYREHSAWVVKTSLQKQSDGNIVSVSVNGDIRYFDPRMPECLNSLQIVKGLTALDIHPQANLFACGSMNQFIAIYNGNGELISNIKYYDGFMGQRIGAISCLAFHPYWPHLAVGSNDYYMSIYSVEKRAR

Target ORF information:

RefSeq Version	XM_012971555.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971555.3

ORF Insert Sequence:

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTGGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG GAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACG CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTC TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTTTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TTGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGAGTAA GACTGCTGGG AGTGCCATTA CATTTTCTCC TGGGAATCTA 
AGTACAAGTA GTAGCGCAAG CAGTACGTTA GGAAGCCCAG ATAATGAAGA GTACATCTTG 
TCCTTTGAGA CCATTGATAA GATGAGGCGT GTCAGTTCTT ACTCCTCTCT CAATTCCCTC 
ATAGGTGTCT CGTTCAATAG TGTCTACACT CAAATTTGGA GAGTGCTTCT CCACCTTGCT 
GCTGACCCAT ACCCTGAAGT CTCGGACCTT GCAATGAAAG TGCTAAACAG CATTGCTCAT 
AAGGCTACAG TGAATGCTCG CCCACAGCGG ATCCTCGATG CCTCCTCACT CACGCAGTCT 
GCTCCCGCCA GTCCCACCAA CAAAGGGATG CACATTCACC AAGTGGGTGG TTCCCCTCCG 
ACTCCCAGTG CCAGCAGTTC CAGTCTCACC AACGATGTAA CGAAACCACC TGTTAGTAGG 
GATATTCCCT CTAATCGACC TGCCAATGCT GGGGGCAATG TTGGGGTGCA GTACACGCCT 
CATTCCCACC AGTTTCCTCG TACAAGGAAA ATGTTCGACA AAGGACCAGA CCAGCCTGGA 
GATGATGGAG ATGAAGCCAT TGTACACAAG AACTTCACTG TAGGATCTTT TCAGACAGGC 
TTTTGTGATT GGAGTGCCAA ATATTTTGCC CAGCCAGTCA TGAAGATGCC AGAGGAACAT 
GACATGGAGA GTCAGGTACG TAAAGAACGA GAGTGGCGAT TTCTACGAAA TACAAGGGTA 
CGGAAACAGG CCCAGCGCCT TATACAGAAA GGAATCTCAA GACTCGATGA CCAGATCTTT 
ATCAACCGGA ATCCAGGAGT TCCTTCTGTT GTGAAGTTCC ATCCTTTTAC CTCAAGCATT 
GCTGTAGCAG ACAAGGACAG CATTTGTTTC TGGGACTGGG AGAAAGGAGA GAAGCTGGAT 
TACTTTCACA ATGGTAACCC TCGTTATACT CGTATAACAG CAGTCGAATA TCTGAATGGC 
CAAGATTGCT CTTTGCTGCT TACTGCTACA GATGATGGAG CCATCCGCGT GTGGAAGAAC 
TTTGCTGACT TAGAGAAAAA TCCAGAGATG GTGACAGCTT GGCAGGGACT GTCCGATATG 
CTGCCGACCA CACGAGGGGC TGGCATGGTG GTGGATTGGG AGCAAGAAAC AGGTCTACTC 
ATCACATCTG GAGACGTGCG AATTATCCGG CTATGGGACA CAGACAGAGA GACAAAAGTG 
CAGGATATAC CCACTGGAGC AGACAGTTGT GTGACCAGCC TGTCATGTGA CTCTCATCGT 
TCCCTTATTG TGGCTGGACT CGGAGATGGT TCAGTGCGAG TGTTTGATCG CAGGATGGCA 
CTGAATGAAT GCCGTGTAAT GACGTACCGA GAGCACTCAG CCTGGGTAGT AAAGACTTCT 
CTCCAAAAGC AGTCCGATGG GAATATTGTG AGCGTCAGTG TAAATGGAGA TATCCGTTAC 
TTCGACCCTC GTATGCCAGA ATGTTTAAAC TCACTGCAGA TTGTGAAGGG TCTTACTGCG 
TTGGATATCC ACCCACAGGC TAACCTGTTT GCATGTGGTT CAATGAACCA GTTCATCGCT 
ATTTACAATG GAAATGGAGA GCTGATCAGC AACATAAAGT ACTACGATGG ATTTATGGGA 
CAAAGAATAG GAGCCATTAG CTGCCTGGCC TTTCATCCTT ACTGGCCCCA CCTGGCCGTT 
GGCAGTAATG ACTATTATAT GTCTATTTAC TCAGTGGAGA AGCGAGCGAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rptor ( XM_012971556.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827010.1 Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X4, mRNA.

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CloneID	OXa49978
Clone ID Related Accession (Same CDS sequence)	XM_012971556.3
Accession Version	XM_012971556.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulatory-associated protein of mTOR isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971556.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTGGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG GAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACG CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTC TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTTTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TTGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGACTGG GAGTGCCATT ACATTTTCTC CTGGGAATCT AAGTACAAGT 
AGTAGCGCAA GCAGTACGTT AGGAAGCCCA GATAATGAAG AGTACATCTT GTCCTTTGAG 
ACCATTGATA AGATGAGGCG TGTCAGTTCT TACTCCTCTC TCAATTCCCT CATAGGTGTC 
TCGTTCAATA GTGTCTACAC TCAAATTTGG AGAGTGCTTC TCCACCTTGC TGCTGACCCA 
TACCCTGAAG TCTCGGACCT TGCAATGAAA GTGCTAAACA GCATTGCTCA TAAGGCTACA 
GTGAATGCTC GCCCACAGCG GATCCTCGAT GCCTCCTCAC TCACGCAGTC TGCTCCCGCC 
AGTCCCACCA ACAAAGGGAT GCACATTCAC CAAGTGGGTG GTTCCCCTCC GACTCCCAGT 
GCCAGCAGTT CCAGTCTCAC CAACGATGTA ACGAAACCAC CTGTTAGTAG GGATATTCCC 
TCTAATCGAC CTGCCAATGC TGGGGGCAAT GTTGGGGTGC AGTACACGCC TCATTCCCAC 
CAGTTTCCTC GTACAAGGAA AATGTTCGAC AAAGGACCAG ACCAGCCTGG AGATGATGGA 
GATGAAGCCA TTGTACACAA GAACTTCACT GTAGGATCTT TTCAGACAGG CTTTTGTGAT 
TGGAGTGCCA AATATTTTGC CCAGCCAGTC ATGAAGATGC CAGAGGAACA TGACATGGAG 
AGTCAGGTAC GTAAAGAACG AGAGTGGCGA TTTCTACGAA ATACAAGGGT ACGGAAACAG 
GCCCAGCGCC TTATACAGAA AGGAATCTCA AGACTCGATG ACCAGATCTT TATCAACCGG 
AATCCAGGAG TTCCTTCTGT TGTGAAGTTC CATCCTTTTA CCTCAAGCAT TGCTGTAGCA 
GACAAGGACA GCATTTGTTT CTGGGACTGG GAGAAAGGAG AGAAGCTGGA TTACTTTCAC 
AATGGTAACC CTCGTTATAC TCGTATAACA GCAGTCGAAT ATCTGAATGG CCAAGATTGC 
TCTTTGCTGC TTACTGCTAC AGATGATGGA GCCATCCGCG TGTGGAAGAA CTTTGCTGAC 
TTAGAGAAAA ATCCAGAGAT GGTGACAGCT TGGCAGGGAC TGTCCGATAT GCTGCCGACC 
ACACGAGGGG CTGGCATGGT GGTGGATTGG GAGCAAGAAA CAGGTCTACT CATCACATCT 
GGAGACGTGC GAATTATCCG GCTATGGGAC ACAGACAGAG AGACAAAAGT GCAGGATATA 
CCCACTGGAG CAGACAGTTG TGTGACCAGC CTGTCATGTG ACTCTCATCG TTCCCTTATT 
GTGGCTGGAC TCGGAGATGG TTCAGTGCGA GTGTTTGATC GCAGGATGGC ACTGAATGAA 
TGCCGTGTAA TGACGTACCG AGAGCACTCA GCCTGGGTAG TAAAGACTTC TCTCCAAAAG 
CAGTCCGATG GGAATATTGT GAGCGTCAGT GTAAATGGAG ATATCCGTTA CTTCGACCCT 
CGTATGCCAG AATGTTTAAA CTCACTGCAG ATTGTGAAGG GTCTTACTGC GTTGGATATC 
CACCCACAGG CTAACCTGTT TGCATGTGGT TCAATGAACC AGTTCATCGC TATTTACAAT 
GGAAATGGAG AGCTGATCAG CAACATAAAG TACTACGATG GATTTATGGG ACAAAGAATA 
GGAGCCATTA GCTGCCTGGC CTTTCATCCT TACTGGCCCC ACCTGGCCGT TGGCAGTAAT 
GACTATTATA TGTCTATTTA CTCAGTGGAG AAGCGAGCGA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827010.1
CDS	116..4120
Translation	MELHTPALGLVGEDEETDLSDWNLPSAFMKKRHCEKIEGCKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLAMGPQKALETIGANLQKQYETWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPSHPLAQMPLPPSMKNCIQLAACDAAELLPMNPDLPADLFTSCLTTPIKIALRWFCMQKSVRLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCSPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTSFILAVIVNNYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDAARWCGVRDSAHEKLYSLLSDPMPEVRSSAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPMVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPASTEGGSLTPIRDSPGTPRLRSVSSYGNIRAVTTPRNLNKSLQNLSLSEETGSAITFSPGNLSTSSSASSTLGSPDNEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDLAMKVLNSIAHKATVNARPQRILDASSLTQSAPASPTNKGMHIHQVGGSPPTPSASSSSLTNDVTKPPVSRDIPSNRPANAGGNVGVQYTPHSHQFPRTRKMFDKGPDQPGDDGDEAIVHKNFTVGSFQTGFCDWSAKYFAQPVMKMPEEHDMESQVRKEREWRFLRNTRVRKQAQRLIQKGISRLDDQIFINRNPGVPSVVKFHPFTSSIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRITAVEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLITSGDVRIIRLWDTDRETKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSVRVFDRRMALNECRVMTYREHSAWVVKTSLQKQSDGNIVSVSVNGDIRYFDPRMPECLNSLQIVKGLTALDIHPQANLFACGSMNQFIAIYNGNGELISNIKYYDGFMGQRIGAISCLAFHPYWPHLAVGSNDYYMSIYSVEKRAR

Target ORF information:

RefSeq Version	XM_012971556.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971556.3

ORF Insert Sequence:

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTGGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG GAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACG CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTC TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTTTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TTGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGACTGG GAGTGCCATT ACATTTTCTC CTGGGAATCT AAGTACAAGT 
AGTAGCGCAA GCAGTACGTT AGGAAGCCCA GATAATGAAG AGTACATCTT GTCCTTTGAG 
ACCATTGATA AGATGAGGCG TGTCAGTTCT TACTCCTCTC TCAATTCCCT CATAGGTGTC 
TCGTTCAATA GTGTCTACAC TCAAATTTGG AGAGTGCTTC TCCACCTTGC TGCTGACCCA 
TACCCTGAAG TCTCGGACCT TGCAATGAAA GTGCTAAACA GCATTGCTCA TAAGGCTACA 
GTGAATGCTC GCCCACAGCG GATCCTCGAT GCCTCCTCAC TCACGCAGTC TGCTCCCGCC 
AGTCCCACCA ACAAAGGGAT GCACATTCAC CAAGTGGGTG GTTCCCCTCC GACTCCCAGT 
GCCAGCAGTT CCAGTCTCAC CAACGATGTA ACGAAACCAC CTGTTAGTAG GGATATTCCC 
TCTAATCGAC CTGCCAATGC TGGGGGCAAT GTTGGGGTGC AGTACACGCC TCATTCCCAC 
CAGTTTCCTC GTACAAGGAA AATGTTCGAC AAAGGACCAG ACCAGCCTGG AGATGATGGA 
GATGAAGCCA TTGTACACAA GAACTTCACT GTAGGATCTT TTCAGACAGG CTTTTGTGAT 
TGGAGTGCCA AATATTTTGC CCAGCCAGTC ATGAAGATGC CAGAGGAACA TGACATGGAG 
AGTCAGGTAC GTAAAGAACG AGAGTGGCGA TTTCTACGAA ATACAAGGGT ACGGAAACAG 
GCCCAGCGCC TTATACAGAA AGGAATCTCA AGACTCGATG ACCAGATCTT TATCAACCGG 
AATCCAGGAG TTCCTTCTGT TGTGAAGTTC CATCCTTTTA CCTCAAGCAT TGCTGTAGCA 
GACAAGGACA GCATTTGTTT CTGGGACTGG GAGAAAGGAG AGAAGCTGGA TTACTTTCAC 
AATGGTAACC CTCGTTATAC TCGTATAACA GCAGTCGAAT ATCTGAATGG CCAAGATTGC 
TCTTTGCTGC TTACTGCTAC AGATGATGGA GCCATCCGCG TGTGGAAGAA CTTTGCTGAC 
TTAGAGAAAA ATCCAGAGAT GGTGACAGCT TGGCAGGGAC TGTCCGATAT GCTGCCGACC 
ACACGAGGGG CTGGCATGGT GGTGGATTGG GAGCAAGAAA CAGGTCTACT CATCACATCT 
GGAGACGTGC GAATTATCCG GCTATGGGAC ACAGACAGAG AGACAAAAGT GCAGGATATA 
CCCACTGGAG CAGACAGTTG TGTGACCAGC CTGTCATGTG ACTCTCATCG TTCCCTTATT 
GTGGCTGGAC TCGGAGATGG TTCAGTGCGA GTGTTTGATC GCAGGATGGC ACTGAATGAA 
TGCCGTGTAA TGACGTACCG AGAGCACTCA GCCTGGGTAG TAAAGACTTC TCTCCAAAAG 
CAGTCCGATG GGAATATTGT GAGCGTCAGT GTAAATGGAG ATATCCGTTA CTTCGACCCT 
CGTATGCCAG AATGTTTAAA CTCACTGCAG ATTGTGAAGG GTCTTACTGC GTTGGATATC 
CACCCACAGG CTAACCTGTT TGCATGTGGT TCAATGAACC AGTTCATCGC TATTTACAAT 
GGAAATGGAG AGCTGATCAG CAACATAAAG TACTACGATG GATTTATGGG ACAAAGAATA 
GGAGCCATTA GCTGCCTGGC CTTTCATCCT TACTGGCCCC ACCTGGCCGT TGGCAGTAAT 
GACTATTATA TGTCTATTTA CTCAGTGGAG AAGCGAGCGA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rptor ( NM_001130371.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001123843.1 Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), mRNA.

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CloneID	OXa24060
Clone ID Related Accession (Same CDS sequence)	NM_001130371.1
Accession Version	NM_001130371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulatory-associated protein of mTOR
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167708.1. ##Evidence-Data-START## Transcript exon combination :: BC167708.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGGCTC GGTACAAACA GAGTCTAGAT 
CCAACTGTGG ATGAGGTTAA AAAACTCTGC ACTTCGCTGA GAAGAAATGC CAAGGAGGAA 
AGGGTGCTGT TCCATTATAA TGGACATGGG GTTCCCCGAC CTACAGTCAA CGGGGAAATC 
TGGGTCTTTA ATAAGAATTA CACACAGTAT ATTCCTCTTT CTATATATGA CCTACAGACC 
TGGATGGGAA GCCCATCTAT CTTTGTATAT GACTGCTCAA ATGCCGGCCT CATTGTCAAG 
TCTTTCAAGC AATTTGCACT ACAGAGAGAG CAAGAGCTTG AGGTTGCTGC TATTAACCCA 
AGCCACCCAT TAGCACAGAT GCCATTGCCA CCATCAATGA AGAATTGCAT CCAGTTGGCA 
GCTTGTGATG CAGCTGAACT TTTGCCTATG AACCCCGACC TTCCTGCTGA CCTTTTCACG 
TCTTGCTTGA CCACACCTAT TAAGATTGCC CTGAGATGGT TTTGTATGCA GAAAAGTGTG 
CGATTGGTGC CTGGTGTTAC TCTGGATCTT ATAGAGAAAA TTCCTGGGCG ATTAAATGAT 
AGGAGGACAC CACTGGGAGA GCTGAACTGG ATCTTTACAG CAATCACAGA TACTATAGCA 
TGGAACGTTC TGCCTAGAGA CCTATTTCAG AAGCTCTTTA GACAGGACCT ATTAGTTGCC 
AGTCTCTTCC GGAACTTTCT TCTGGCCGAG AGGATCATGA GATCATACAA TTGCAGCCCA 
GTGAGCAGCC CTCGTTTGCC ACCCACCTAC ATGCATGCTA TGTGGCAGGC ATGGGATCTT 
GCTGTCGATA TATGTCTTTC TCAGCTACCC ACCATTATTG AGGAGGGAAC TGCTTTCCGG 
CACAGCCCTT TCTTTGCTGA GCAGCTGACT GCATTTCAGG TGTGGCTGAC TATGGGTGTT 
GAGAACAGAA ACCCCCCAGA ACAGTTGCCC ATTGTGCTGC AGGTTTTGCT GAGCCAGGTT 
CATAGACTCC GTGCCTTGGA CCTCTTGGGA AGATTCTTAG ATCTGGGTCC CTGGGCTGTC 
AGTCTGGCCC TCTCTGTTGG TATTTTCCCT TATGTGCTAA AGCTGCTCCA AAGCTCAGCT 
CGGGAACTCC GGCCACTGCT TGTCTTTATT TGGGCCAAGA TCCTTGCTGT TGATAGTTCG 
TGCCAAGCAG ACTTAGTAAA AGATAATGGC CATAAATATT TTCTTTCTGT TCTGGCGGAT 
CCATATATGC CGGCTGAGCA CCGTACAATG ACCTCCTTCA TTTTAGCAGT AATTGTTAAC 
AATTATAACA CTGGCCAGGA AGCCTGCCTC CAAGGAAACC TGATCGCTAT CTGCTTGGAG 
CAGCTAAATG ATCCACACCC TCTCCTACGC CAATGGGTTG CTATCTGCCT GGGACGCATC 
TGGCAGAATT TTGATGCTGC ACGTTGGTGT GGGGTACGTG ATAGCGCCCA TGAGAAGCTG 
TACAGCCTCT TGTCTGACCC CATGCCAGAG GTCCGTAGTT CTGCAGTTTT TGCCCTTGGC 
ACCTTCGTTG GGAATTCAGC TGAGAGAACA GATCACTCTA CAACAATAGA TCACAATGTT 
GCAATGATGT TAGCACAGCT TATCAATGAT GGGAGTCCTA TGGTGCGCAA GGAGCTTGTT 
GTAGCTCTTA GTCATCTGGT TGTGCAGTAT GAAAGTAACT TCTGTACAGT TGCCCTCCAG 
TTTATGGAGG AAGAGAAGAA TTACCCCCTG CCCTCGCCAG CTTCCACAGA GGGAGGCAGT 
TTGACACCAA TACGAGATAG CCCTGGAACG CCCCGCTTGA GATCTGTGAG CTCCTACGGT 
AATATCCGGG CAGTGACAAC TCCAAGAAAC TTGAACAAGT CTCTGCAGAA TCTAAGCCTG 
AGTGAAGAGA CTGGGAGTGC CATTACATTT TCTCCTGGGA ATCTAAGTAC AAGTAGTAGC 
GCAAGCAGTA CGTTAGGAAG CCCAGATAAT GAAGAGTACA TCTTGTCCTT TGAGACCATT 
GATAAGATGA GGCGTGTCAG TTCTTACTCC TCTCTCAATT CCCTCATAGG TGTCTCGTTC 
AATAGTGTCT ACACTCAGAT TTGGAGAGTG CTTCTCCACC TTGCTGCTGA CCCATACCCT 
GAAGTCTCGG ACCTTGCAAT GAAAGTGCTA AACAGCATTG CTCATAAGGC TACAGTGAAT 
GCTCGCCCAC AGCGGATCCT CGATGCCTCC TCACTCACGC AGTCTGCTCC AGCCAGTCCC 
ACCAACAAAG GGATGCACAT TCACCAAGTG GGTGGTTCCC CTCCGACTCC CAGTGCCAGC 
AGTTCCAGTC TCACCAACGA TGTAACGAAA CCACCTGTTA GTAGGGATAT TCCCTCTAAT 
CGACCTGCCA ATGCTGGGGG CAATGTTGGG GTGCAGTACA CGCCTCATTC CCACCAGTTT 
CCTCGTACAA GGAAAATGTT CGACAAAGGA CCAGACCAGC CTGGAGATGA TGGAGATGAA 
GCCATTGTAC ACAAGAACTT TACTGTAGGA TCTTTTCAGA CAGGCTTTTG TGATTGGAGT 
GCCAAATATT TTGCCCAGCC AGTCATGAAG ATGCCAGAGG AACATGACAT GGAGAGTCAG 
GTACGTAAAG AACGAGAGTG GCGATTTCTA CGAAATACAA GGGTACGGAA ACAGGCCCAG 
CGCCTTATAC AGAAAGGAAT CTCAAGACTC GATGACCAGA TCTTTATCAA CCGGAATCCA 
GGAGTTCCTT CTGTTGTGAA GTTCCATCCT TTTACCTCAA GCATTGCTGT AGCAGACAAG 
GACAGCATTT GTTTCTGGGA CTGGGAGAAA GGAGAGAAGC TGGATTACTT TCACAATGGT 
AACCCTCGTT ATACTCGTAT AACAGCAGTC GAATATCTGA ATGGCCAGGA TTGCTCTTTG 
CTGCTTACTG CTACAGATGA TGGAGCCATC CGCGTGTGGA AGAACTTTGC TGACTTAGAG 
AAAAATCCAG AGATGGTGAC AGCTTGGCAG GGACTGTCCG ATATGCTGCC GACCACACGA 
GGCCTGGCTC GAAGAGTTTC CATTTATCTT GACAGGAGTA AACAGGGGGG GGCTGGCATG 
GTGGTGGATT GGGAGCAAGA AACAGGTCTA CTCATCACAT CTGGAGACGT GCGAATTATC 
CGGCTATGGG ACACAGACAG AGAGACAAAA GTGCAGGATA TACCCACTGG AGCAGACAGT 
TGTGTGACCA GCCTGTCATG TGACTCTCAT CGTTCCCTTA TTGTGGCTGG ACTCGGAGAT 
GGTTCAGTGC GAGTGTTTGA TCGCAGGATG GCACTGAATG AATGCCGTGT AATGACGTAC 
CGAGAGCACT CAGCCTGGGT AGTAAAGACT TCTCTCCAAA AGCAGTCCGA TGGGAATATT 
GTGAGCGTCA GTGTAAATGG AGATATCCGT TACTTCGACC CTCGTATGCC AGAATGTTTA 
AACTCACTGC AGATTGTGAA GGGTCTTACT GCGTTGGATA TCCACCCACA GGCTAACCTG 
TTTGCATGTG GTTCAATGAA CCAGTTCATC GCTATTTACA ATGGAAATGG AGAGCTGATC 
AGCAACATAA AGTACTACGA TGGATTTATG GGACAAAGAA TAGGAGCCAT TAGCTGCCTG 
GCCTTTCATC CTTACTGGCC CCACCTGGCC GTTGGCAGTA ATGACTATTA TATGTCTATT 
TACTCAGTGG AGAAGCGAGC GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123843.1
CDS	89..3955
Translation	MELHTPALGLVGEDEETDLSDWNLPSAFMKKRHCEKIEGCKSLAQSWRMKDRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPSHPLAQMPLPPSMKNCIQLAACDAAELLPMNPDLPADLFTSCLTTPIKIALRWFCMQKSVRLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCSPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTSFILAVIVNNYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDAARWCGVRDSAHEKLYSLLSDPMPEVRSSAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPMVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPASTEGGSLTPIRDSPGTPRLRSVSSYGNIRAVTTPRNLNKSLQNLSLSEETGSAITFSPGNLSTSSSASSTLGSPDNEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDLAMKVLNSIAHKATVNARPQRILDASSLTQSAPASPTNKGMHIHQVGGSPPTPSASSSSLTNDVTKPPVSRDIPSNRPANAGGNVGVQYTPHSHQFPRTRKMFDKGPDQPGDDGDEAIVHKNFTVGSFQTGFCDWSAKYFAQPVMKMPEEHDMESQVRKEREWRFLRNTRVRKQAQRLIQKGISRLDDQIFINRNPGVPSVVKFHPFTSSIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRITAVEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGLARRVSIYLDRSKQGGAGMVVDWEQETGLLITSGDVRIIRLWDTDRETKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSVRVFDRRMALNECRVMTYREHSAWVVKTSLQKQSDGNIVSVSVNGDIRYFDPRMPECLNSLQIVKGLTALDIHPQANLFACGSMNQFIAIYNGNGELISNIKYYDGFMGQRIGAISCLAFHPYWPHLAVGSNDYYMSIYSVEKRAR

Target ORF information:

RefSeq Version	NM_001130371.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130371.1

ORF Insert Sequence:

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGGCTC GGTACAAACA GAGTCTAGAT 
CCAACTGTGG ATGAGGTTAA AAAACTCTGC ACTTCGCTGA GAAGAAATGC CAAGGAGGAA 
AGGGTGCTGT TCCATTATAA TGGACATGGG GTTCCCCGAC CTACAGTCAA CGGGGAAATC 
TGGGTCTTTA ATAAGAATTA CACACAGTAT ATTCCTCTTT CTATATATGA CCTACAGACC 
TGGATGGGAA GCCCATCTAT CTTTGTATAT GACTGCTCAA ATGCCGGCCT CATTGTCAAG 
TCTTTCAAGC AATTTGCACT ACAGAGAGAG CAAGAGCTTG AGGTTGCTGC TATTAACCCA 
AGCCACCCAT TAGCACAGAT GCCATTGCCA CCATCAATGA AGAATTGCAT CCAGTTGGCA 
GCTTGTGATG CAGCTGAACT TTTGCCTATG AACCCCGACC TTCCTGCTGA CCTTTTCACG 
TCTTGCTTGA CCACACCTAT TAAGATTGCC CTGAGATGGT TTTGTATGCA GAAAAGTGTG 
CGATTGGTGC CTGGTGTTAC TCTGGATCTT ATAGAGAAAA TTCCTGGGCG ATTAAATGAT 
AGGAGGACAC CACTGGGAGA GCTGAACTGG ATCTTTACAG CAATCACAGA TACTATAGCA 
TGGAACGTTC TGCCTAGAGA CCTATTTCAG AAGCTCTTTA GACAGGACCT ATTAGTTGCC 
AGTCTCTTCC GGAACTTTCT TCTGGCCGAG AGGATCATGA GATCATACAA TTGCAGCCCA 
GTGAGCAGCC CTCGTTTGCC ACCCACCTAC ATGCATGCTA TGTGGCAGGC ATGGGATCTT 
GCTGTCGATA TATGTCTTTC TCAGCTACCC ACCATTATTG AGGAGGGAAC TGCTTTCCGG 
CACAGCCCTT TCTTTGCTGA GCAGCTGACT GCATTTCAGG TGTGGCTGAC TATGGGTGTT 
GAGAACAGAA ACCCCCCAGA ACAGTTGCCC ATTGTGCTGC AGGTTTTGCT GAGCCAGGTT 
CATAGACTCC GTGCCTTGGA CCTCTTGGGA AGATTCTTAG ATCTGGGTCC CTGGGCTGTC 
AGTCTGGCCC TCTCTGTTGG TATTTTCCCT TATGTGCTAA AGCTGCTCCA AAGCTCAGCT 
CGGGAACTCC GGCCACTGCT TGTCTTTATT TGGGCCAAGA TCCTTGCTGT TGATAGTTCG 
TGCCAAGCAG ACTTAGTAAA AGATAATGGC CATAAATATT TTCTTTCTGT TCTGGCGGAT 
CCATATATGC CGGCTGAGCA CCGTACAATG ACCTCCTTCA TTTTAGCAGT AATTGTTAAC 
AATTATAACA CTGGCCAGGA AGCCTGCCTC CAAGGAAACC TGATCGCTAT CTGCTTGGAG 
CAGCTAAATG ATCCACACCC TCTCCTACGC CAATGGGTTG CTATCTGCCT GGGACGCATC 
TGGCAGAATT TTGATGCTGC ACGTTGGTGT GGGGTACGTG ATAGCGCCCA TGAGAAGCTG 
TACAGCCTCT TGTCTGACCC CATGCCAGAG GTCCGTAGTT CTGCAGTTTT TGCCCTTGGC 
ACCTTCGTTG GGAATTCAGC TGAGAGAACA GATCACTCTA CAACAATAGA TCACAATGTT 
GCAATGATGT TAGCACAGCT TATCAATGAT GGGAGTCCTA TGGTGCGCAA GGAGCTTGTT 
GTAGCTCTTA GTCATCTGGT TGTGCAGTAT GAAAGTAACT TCTGTACAGT TGCCCTCCAG 
TTTATGGAGG AAGAGAAGAA TTACCCCCTG CCCTCGCCAG CTTCCACAGA GGGAGGCAGT 
TTGACACCAA TACGAGATAG CCCTGGAACG CCCCGCTTGA GATCTGTGAG CTCCTACGGT 
AATATCCGGG CAGTGACAAC TCCAAGAAAC TTGAACAAGT CTCTGCAGAA TCTAAGCCTG 
AGTGAAGAGA CTGGGAGTGC CATTACATTT TCTCCTGGGA ATCTAAGTAC AAGTAGTAGC 
GCAAGCAGTA CGTTAGGAAG CCCAGATAAT GAAGAGTACA TCTTGTCCTT TGAGACCATT 
GATAAGATGA GGCGTGTCAG TTCTTACTCC TCTCTCAATT CCCTCATAGG TGTCTCGTTC 
AATAGTGTCT ACACTCAGAT TTGGAGAGTG CTTCTCCACC TTGCTGCTGA CCCATACCCT 
GAAGTCTCGG ACCTTGCAAT GAAAGTGCTA AACAGCATTG CTCATAAGGC TACAGTGAAT 
GCTCGCCCAC AGCGGATCCT CGATGCCTCC TCACTCACGC AGTCTGCTCC AGCCAGTCCC 
ACCAACAAAG GGATGCACAT TCACCAAGTG GGTGGTTCCC CTCCGACTCC CAGTGCCAGC 
AGTTCCAGTC TCACCAACGA TGTAACGAAA CCACCTGTTA GTAGGGATAT TCCCTCTAAT 
CGACCTGCCA ATGCTGGGGG CAATGTTGGG GTGCAGTACA CGCCTCATTC CCACCAGTTT 
CCTCGTACAA GGAAAATGTT CGACAAAGGA CCAGACCAGC CTGGAGATGA TGGAGATGAA 
GCCATTGTAC ACAAGAACTT TACTGTAGGA TCTTTTCAGA CAGGCTTTTG TGATTGGAGT 
GCCAAATATT TTGCCCAGCC AGTCATGAAG ATGCCAGAGG AACATGACAT GGAGAGTCAG 
GTACGTAAAG AACGAGAGTG GCGATTTCTA CGAAATACAA GGGTACGGAA ACAGGCCCAG 
CGCCTTATAC AGAAAGGAAT CTCAAGACTC GATGACCAGA TCTTTATCAA CCGGAATCCA 
GGAGTTCCTT CTGTTGTGAA GTTCCATCCT TTTACCTCAA GCATTGCTGT AGCAGACAAG 
GACAGCATTT GTTTCTGGGA CTGGGAGAAA GGAGAGAAGC TGGATTACTT TCACAATGGT 
AACCCTCGTT ATACTCGTAT AACAGCAGTC GAATATCTGA ATGGCCAGGA TTGCTCTTTG 
CTGCTTACTG CTACAGATGA TGGAGCCATC CGCGTGTGGA AGAACTTTGC TGACTTAGAG 
AAAAATCCAG AGATGGTGAC AGCTTGGCAG GGACTGTCCG ATATGCTGCC GACCACACGA 
GGCCTGGCTC GAAGAGTTTC CATTTATCTT GACAGGAGTA AACAGGGGGG GGCTGGCATG 
GTGGTGGATT GGGAGCAAGA AACAGGTCTA CTCATCACAT CTGGAGACGT GCGAATTATC 
CGGCTATGGG ACACAGACAG AGAGACAAAA GTGCAGGATA TACCCACTGG AGCAGACAGT 
TGTGTGACCA GCCTGTCATG TGACTCTCAT CGTTCCCTTA TTGTGGCTGG ACTCGGAGAT 
GGTTCAGTGC GAGTGTTTGA TCGCAGGATG GCACTGAATG AATGCCGTGT AATGACGTAC 
CGAGAGCACT CAGCCTGGGT AGTAAAGACT TCTCTCCAAA AGCAGTCCGA TGGGAATATT 
GTGAGCGTCA GTGTAAATGG AGATATCCGT TACTTCGACC CTCGTATGCC AGAATGTTTA 
AACTCACTGC AGATTGTGAA GGGTCTTACT GCGTTGGATA TCCACCCACA GGCTAACCTG 
TTTGCATGTG GTTCAATGAA CCAGTTCATC GCTATTTACA ATGGAAATGG AGAGCTGATC 
AGCAACATAA AGTACTACGA TGGATTTATG GGACAAAGAA TAGGAGCCAT TAGCTGCCTG 
GCCTTTCATC CTTACTGGCC CCACCTGGCC GTTGGCAGTA ATGACTATTA TATGTCTATT 
TACTCAGTGG AGAAGCGAGC GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rptor ( XM_012971557.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827011.1 Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X5, mRNA.

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CloneID	OXa32468
Clone ID Related Accession (Same CDS sequence)	XM_012971557.2
Accession Version	XM_012971557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulatory-associated protein of mTOR isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTAGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG AAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GGTTGCTGCT ATTAACCCAA GCCACCCATT AGCACAGATG 
CCATTGCCAC CATCAATGAA GAATTGCATC CAGTTGGCAG CTTGTGATGC AGCTGAACTT 
TTGCCTATGA ACCCCGACCT TCCTGCTGAC CTTTTCACTT CTTGCTTGAC CACACCTATT 
AAGATTGCCC TGAGATGGTT TTGTATGCAG AAAAGTGTGC GATTGGTGCC TGGTGTTACT 
CTGGATCTTA TAGAGAAAAT TCCTGGGCGA TTAAATGATA GGAGGACACC ACTGGGAGAG 
CTGAACTGGA TCTTTACAGC AATCACAGAT ACTATAGCAT GGAACGTTCT GCCTAGAGAC 
CTATTTCAGA AGCTCTTTAG ACAGGACCTA TTAGTTGCCA GTCTCTTCCG GAACTTTCTT 
CTGGCCGAGA GGATCATGAG ATCATACAAT TGCAGCCCAG TGAGCAGCCC TCGTTTGCCA 
CCCACCTACA TGCATGCTAT GTGGCAGGCA TGGGATCTTG CTGTCGATAT ATGTCTTTCT 
CAGCTACCCA CCATTATTGA GGAGGGAACT GCTTTCCGGC ACAGCCCTTT CTTTGCTGAG 
CAGCTGACTG CATTTCAGGT GTGGCTGACT ATGGGTGTTG AGAACAGAAA CCCCCCAGAA 
CAGTTGCCCA TTGTGCTGCA GGTTTTGCTG AGCCAGGTTC ATAGACTCCG TGCCTTGGAC 
CTTTTGGGAA GATTCTTGGA TCTGGGTCCC TGGGCTGTCA GTCTGGCCCT CTCTGTTGGT 
ATTTTCCCTT ATGTGCTAAA GCTGCTCCAA AGCTCAGCTC GGGAACTCCG GCCACTGCTT 
GTCTTTATTT GGGCCAAGAT CCTTGCTGTT GATAGTTCGT GCCAAGCAGA CTTAGTAAAA 
GATAATGGCC ATAAATATTT TCTTTCTGTT CTGGCGGATC CATATATGCC GGCTGAGCAC 
CGTACAATGA CCTCCTTCAT TTTAGCAGTA ATTGTTAACA ATTATAACAC TGGCCAGGAA 
GCCTGCCTCC AAGGAAACCT GATCGCTATC TGCTTGGAGC AGCTAAATGA TCCACACCCT 
CTCCTACGCC AATGGGTTGC TATCTGCCTG GGACGCATCT GGCAGAATTT TGATGCTGCA 
CGTTGGTGTG GGGTACGTGA TAGCGCCCAT GAGAAGCTGT ACAGCCTCTT GTCTGACCCC 
ATGCCAGAGG TCCGTAGTTC TGCAGTTTTT GCCCTTGGCA CCTTCGTTGG GAATTCAGCT 
GAGAGAACAG ATCACTCTAC AACAATAGAT CACAATGTTG CAATGATGTT AGCACAGCTT 
ATCAATGATG GGAGTCCTAT GGTGCGCAAG GAGCTTGTTG TAGCTCTTAG TCATCTGGTT 
GTGCAGTATG AAAGTAACTT CTGTACAGTT GCCCTCCAGT TTATGGAGGA AGAGAAGAAT 
TACCCCCTGC CCTCGCCAGC TTCCACAGAG GGAGGCAGTT TGACACCAAT ACGAGATAGC 
CCTGGAACGC CCCGCTTGAG ATCTGTGAGC TCCTACGGTA ATATCCGGGC AGTGACAACT 
CCAAGAAACT TGAACAAGTC TCTGCAGAAT CTAAGCCTGA GTGAAGAGAG TAAGACTGCT 
GGGAGTGCCA TTACATTTTC TCCTGGGAAT CTAAGTACAA GTAGTAGCGC AAGCAGTACG 
TTAGGAAGCC CAGATAATGA AGAGTACATC TTGTCCTTTG AGACCATTGA TAAGATGAGG 
CGTGTCAGTT CTTACTCCTC TCTCAATTCC CTCATAGGTG TCTCGTTCAA TAGTGTCTAC 
ACTCAGATTT GGAGAGTGCT TCTCCACCTT GCTGCTGACC CATACCCTGA AGTCTCGGAC 
CTTGCAATGA AAGTGCTAAA CAGCATTGCT CATAAGGCTA CAGTGAATGC TCGCCCACAG 
CGGATCCTCG ATGCCTCCTC ACTCACGCAG TCTGCTCCCG CCAGTCCCAC CAACAAAGGG 
ATGCACATTC ACCAAGTGGG TGGTTCCCCT CCGACTCCCA GTGCCAGCAG TTCCAGTCTC 
ACCAACGATG TAACGAAACC ACCTGTCAGT AGGGATATTC CCTCTAATCG ACCTGCCAAT 
GCTGGGGGCA ATGTTGGGGT GCAGTACACG CCTCATTCCC ACCAGTTTCC TCGTACAAGG 
AAAATGTTCG ACAAAGGACC AGACCAGCCT GGAGATGATG GAGATGAAGC CATTGTACAC 
AAGAACTTTA CTGTAGGATC TTTTCAGACA GGCTTTTGTG ATTGGAGTGC CAAATATTTT 
GCCCAGCCAG TCATGAAGAT GCCAGAGGAA CATGACATGG AGAGTCAGGT ACGTAAAGAA 
CGAGAGTGGC GATTTCTACG AAATACAAGG GTACGGAAAC AGGCCCAGCG CCTTATACAG 
AAAGGAATCT CAAGACTCGA TGACCAGATC TTTATCAACC GGAATCCAGG AGTTCCTTCT 
GTTGTGAAGT TCCATCCTTT TACCTCAAGC ATTGCTGTAG CAGACAAGGA CAGCATTTGT 
TTCTGGGACT GGGAGAAAGG AGAGAAGCTG GATTACTTTC ACAATGGTAA CCCTCGTTAT 
ACTCGTATAA CAGCAGTCGA ATATCTGAAT GGCCAAGATT GCTCTTTGCT GCTTACTGCT 
ACAGATGATG GAGCCATCCG CGTGTGGAAG AACTTTGCTG ACTTAGAGAA AAATCCAGAG 
ATGGTGACAG CTTGGCAGGG ACTGTCCGAT ATGCTGCCGA CCACACGAGG CCTGGCTCGA 
AGAGTTTCCA TTTATCTTGA CAGGAGTAAA CAGGGGGGGG CTGGCATGGT GGTGGATTGG 
GAGCAAGAAA CAGGTCTACT CATCACATCT GGAGACGTGC GAATTATCCG GCTATGGGAC 
ACAGACAGAG AGACAAAAGT GCAGGATATA CCCACTGGAG CAGACAGTTG TGTGACCAGC 
CTGTCATGTG ACTCTCATCG TTCCCTTATT GTGGCTGGAC TCGGAGATGG TTCAGTGCGA 
GTGTTTGATC GCAGGATGGC ACTGAATGAA TGCCGTGTAA TGACGTACCG AGAGCACTCA 
GCCTGGGTAG TAAAGACTTC TCTCCAAAAG CAGTCCGATG GGAATATTGT GAGCGTCAGT 
GTAAATGGAG ATATCCGTTA CTTCGACCCT CGTATGCCAG AATGTTTAAA CTCACTGCAG 
ATTGTGAAGG GTCTTACTGC GTTGGATATC CACCCACAGG CTAACCTGTT TGCATGTGGT 
TCAATGAACC AGTTCATCGC TATTTACAAT GGAAATGGAG AGCTGATCAG CAACATAAAG 
TACTACGATG GATTTATGGG ACAAAGAATA GGAGCCATTA GCTGCCTGGC CTTTCATCCT 
TACTGGCCCC ACCTGGCCGT TGGCAGTAAT GACTATTATA TGTCTATTTA CTCAGTGGAG 
AAGCGAGCGA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827011.1
CDS	123..4037
Translation	MELHTPALGLVGEDEETDLSDWNLPSAFMKKRHCEKIEGCKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLAMGPQKALETIGANLQKQYETWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKVAAINPSHPLAQMPLPPSMKNCIQLAACDAAELLPMNPDLPADLFTSCLTTPIKIALRWFCMQKSVRLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCSPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTSFILAVIVNNYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDAARWCGVRDSAHEKLYSLLSDPMPEVRSSAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPMVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPASTEGGSLTPIRDSPGTPRLRSVSSYGNIRAVTTPRNLNKSLQNLSLSEESKTAGSAITFSPGNLSTSSSASSTLGSPDNEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDLAMKVLNSIAHKATVNARPQRILDASSLTQSAPASPTNKGMHIHQVGGSPPTPSASSSSLTNDVTKPPVSRDIPSNRPANAGGNVGVQYTPHSHQFPRTRKMFDKGPDQPGDDGDEAIVHKNFTVGSFQTGFCDWSAKYFAQPVMKMPEEHDMESQVRKEREWRFLRNTRVRKQAQRLIQKGISRLDDQIFINRNPGVPSVVKFHPFTSSIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRITAVEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGLARRVSIYLDRSKQGGAGMVVDWEQETGLLITSGDVRIIRLWDTDRETKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSVRVFDRRMALNECRVMTYREHSAWVVKTSLQKQSDGNIVSVSVNGDIRYFDPRMPECLNSLQIVKGLTALDIHPQANLFACGSMNQFIAIYNGNGELISNIKYYDGFMGQRIGAISCLAFHPYWPHLAVGSNDYYMSIYSVEKRAR

Target ORF information:

RefSeq Version	XM_012971557.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971557.2

ORF Insert Sequence:

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTAGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG AAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GGTTGCTGCT ATTAACCCAA GCCACCCATT AGCACAGATG 
CCATTGCCAC CATCAATGAA GAATTGCATC CAGTTGGCAG CTTGTGATGC AGCTGAACTT 
TTGCCTATGA ACCCCGACCT TCCTGCTGAC CTTTTCACTT CTTGCTTGAC CACACCTATT 
AAGATTGCCC TGAGATGGTT TTGTATGCAG AAAAGTGTGC GATTGGTGCC TGGTGTTACT 
CTGGATCTTA TAGAGAAAAT TCCTGGGCGA TTAAATGATA GGAGGACACC ACTGGGAGAG 
CTGAACTGGA TCTTTACAGC AATCACAGAT ACTATAGCAT GGAACGTTCT GCCTAGAGAC 
CTATTTCAGA AGCTCTTTAG ACAGGACCTA TTAGTTGCCA GTCTCTTCCG GAACTTTCTT 
CTGGCCGAGA GGATCATGAG ATCATACAAT TGCAGCCCAG TGAGCAGCCC TCGTTTGCCA 
CCCACCTACA TGCATGCTAT GTGGCAGGCA TGGGATCTTG CTGTCGATAT ATGTCTTTCT 
CAGCTACCCA CCATTATTGA GGAGGGAACT GCTTTCCGGC ACAGCCCTTT CTTTGCTGAG 
CAGCTGACTG CATTTCAGGT GTGGCTGACT ATGGGTGTTG AGAACAGAAA CCCCCCAGAA 
CAGTTGCCCA TTGTGCTGCA GGTTTTGCTG AGCCAGGTTC ATAGACTCCG TGCCTTGGAC 
CTTTTGGGAA GATTCTTGGA TCTGGGTCCC TGGGCTGTCA GTCTGGCCCT CTCTGTTGGT 
ATTTTCCCTT ATGTGCTAAA GCTGCTCCAA AGCTCAGCTC GGGAACTCCG GCCACTGCTT 
GTCTTTATTT GGGCCAAGAT CCTTGCTGTT GATAGTTCGT GCCAAGCAGA CTTAGTAAAA 
GATAATGGCC ATAAATATTT TCTTTCTGTT CTGGCGGATC CATATATGCC GGCTGAGCAC 
CGTACAATGA CCTCCTTCAT TTTAGCAGTA ATTGTTAACA ATTATAACAC TGGCCAGGAA 
GCCTGCCTCC AAGGAAACCT GATCGCTATC TGCTTGGAGC AGCTAAATGA TCCACACCCT 
CTCCTACGCC AATGGGTTGC TATCTGCCTG GGACGCATCT GGCAGAATTT TGATGCTGCA 
CGTTGGTGTG GGGTACGTGA TAGCGCCCAT GAGAAGCTGT ACAGCCTCTT GTCTGACCCC 
ATGCCAGAGG TCCGTAGTTC TGCAGTTTTT GCCCTTGGCA CCTTCGTTGG GAATTCAGCT 
GAGAGAACAG ATCACTCTAC AACAATAGAT CACAATGTTG CAATGATGTT AGCACAGCTT 
ATCAATGATG GGAGTCCTAT GGTGCGCAAG GAGCTTGTTG TAGCTCTTAG TCATCTGGTT 
GTGCAGTATG AAAGTAACTT CTGTACAGTT GCCCTCCAGT TTATGGAGGA AGAGAAGAAT 
TACCCCCTGC CCTCGCCAGC TTCCACAGAG GGAGGCAGTT TGACACCAAT ACGAGATAGC 
CCTGGAACGC CCCGCTTGAG ATCTGTGAGC TCCTACGGTA ATATCCGGGC AGTGACAACT 
CCAAGAAACT TGAACAAGTC TCTGCAGAAT CTAAGCCTGA GTGAAGAGAG TAAGACTGCT 
GGGAGTGCCA TTACATTTTC TCCTGGGAAT CTAAGTACAA GTAGTAGCGC AAGCAGTACG 
TTAGGAAGCC CAGATAATGA AGAGTACATC TTGTCCTTTG AGACCATTGA TAAGATGAGG 
CGTGTCAGTT CTTACTCCTC TCTCAATTCC CTCATAGGTG TCTCGTTCAA TAGTGTCTAC 
ACTCAGATTT GGAGAGTGCT TCTCCACCTT GCTGCTGACC CATACCCTGA AGTCTCGGAC 
CTTGCAATGA AAGTGCTAAA CAGCATTGCT CATAAGGCTA CAGTGAATGC TCGCCCACAG 
CGGATCCTCG ATGCCTCCTC ACTCACGCAG TCTGCTCCCG CCAGTCCCAC CAACAAAGGG 
ATGCACATTC ACCAAGTGGG TGGTTCCCCT CCGACTCCCA GTGCCAGCAG TTCCAGTCTC 
ACCAACGATG TAACGAAACC ACCTGTCAGT AGGGATATTC CCTCTAATCG ACCTGCCAAT 
GCTGGGGGCA ATGTTGGGGT GCAGTACACG CCTCATTCCC ACCAGTTTCC TCGTACAAGG 
AAAATGTTCG ACAAAGGACC AGACCAGCCT GGAGATGATG GAGATGAAGC CATTGTACAC 
AAGAACTTTA CTGTAGGATC TTTTCAGACA GGCTTTTGTG ATTGGAGTGC CAAATATTTT 
GCCCAGCCAG TCATGAAGAT GCCAGAGGAA CATGACATGG AGAGTCAGGT ACGTAAAGAA 
CGAGAGTGGC GATTTCTACG AAATACAAGG GTACGGAAAC AGGCCCAGCG CCTTATACAG 
AAAGGAATCT CAAGACTCGA TGACCAGATC TTTATCAACC GGAATCCAGG AGTTCCTTCT 
GTTGTGAAGT TCCATCCTTT TACCTCAAGC ATTGCTGTAG CAGACAAGGA CAGCATTTGT 
TTCTGGGACT GGGAGAAAGG AGAGAAGCTG GATTACTTTC ACAATGGTAA CCCTCGTTAT 
ACTCGTATAA CAGCAGTCGA ATATCTGAAT GGCCAAGATT GCTCTTTGCT GCTTACTGCT 
ACAGATGATG GAGCCATCCG CGTGTGGAAG AACTTTGCTG ACTTAGAGAA AAATCCAGAG 
ATGGTGACAG CTTGGCAGGG ACTGTCCGAT ATGCTGCCGA CCACACGAGG CCTGGCTCGA 
AGAGTTTCCA TTTATCTTGA CAGGAGTAAA CAGGGGGGGG CTGGCATGGT GGTGGATTGG 
GAGCAAGAAA CAGGTCTACT CATCACATCT GGAGACGTGC GAATTATCCG GCTATGGGAC 
ACAGACAGAG AGACAAAAGT GCAGGATATA CCCACTGGAG CAGACAGTTG TGTGACCAGC 
CTGTCATGTG ACTCTCATCG TTCCCTTATT GTGGCTGGAC TCGGAGATGG TTCAGTGCGA 
GTGTTTGATC GCAGGATGGC ACTGAATGAA TGCCGTGTAA TGACGTACCG AGAGCACTCA 
GCCTGGGTAG TAAAGACTTC TCTCCAAAAG CAGTCCGATG GGAATATTGT GAGCGTCAGT 
GTAAATGGAG ATATCCGTTA CTTCGACCCT CGTATGCCAG AATGTTTAAA CTCACTGCAG 
ATTGTGAAGG GTCTTACTGC GTTGGATATC CACCCACAGG CTAACCTGTT TGCATGTGGT 
TCAATGAACC AGTTCATCGC TATTTACAAT GGAAATGGAG AGCTGATCAG CAACATAAAG 
TACTACGATG GATTTATGGG ACAAAGAATA GGAGCCATTA GCTGCCTGGC CTTTCATCCT 
TACTGGCCCC ACCTGGCCGT TGGCAGTAAT GACTATTATA TGTCTATTTA CTCAGTGGAG 
AAGCGAGCGA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rptor ( XM_012971553.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827007.1 Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X1, mRNA.

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CloneID	OXa32464
Clone ID Related Accession (Same CDS sequence)	XM_012971553.2
Accession Version	XM_012971553.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulatory-associated protein of mTOR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTAGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG AAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACA CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTT TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTCTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TCGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGAGTAA GACTGCTGGG AGTGCCATTA CATTTTCTCC TGGGAATCTA 
AGTACAAGTA GTAGCGCAAG CAGTACGTTA GGAAGCCCAG ATAATGAAGA GTACATCTTG 
TCCTTTGAGA CCATTGATAA GATGAGGCGT GTCAGTTCTT ACTCCTCTCT CAATTCCCTC 
ATAGGTGTCT CGTTCAATAG TGTCTACACT CAGATTTGGA GAGTGCTTCT CCACCTTGCT 
GCTGACCCAT ACCCTGAAGT CTCGGACCTT GCAATGAAAG TGCTAAACAG CATTGCTCAT 
AAGGCTACAG TGAATGCTCG CCCACAGCGG ATCCTCGATG CCTCCTCACT CACGCAGTCT 
GCTCCCGCCA GTCCCACCAA CAAAGGGATG CACATTCACC AAGTGGGTGG TTCCCCTCCG 
ACTCCCAGTG CCAGCAGTTC CAGTCTCACC AACGATGTAA CGAAACCACC TGTCAGTAGG 
GATATTCCCT CTAATCGACC TGCCAATGCT GGGGGCAATG TTGGGGTGCA GTACACGCCT 
CATTCCCACC AGTTTCCTCG TACAAGGAAA ATGTTCGACA AAGGACCAGA CCAGCCTGGA 
GATGATGGAG ATGAAGCCAT TGTACACAAG AACTTTACTG TAGGATCTTT TCAGACAGGC 
TTTTGTGATT GGAGTGCCAA ATATTTTGCC CAGCCAGTCA TGAAGATGCC AGAGGAACAT 
GACATGGAGA GTCAGGTACG TAAAGAACGA GAGTGGCGAT TTCTACGAAA TACAAGGGTA 
CGGAAACAGG CCCAGCGCCT TATACAGAAA GGAATCTCAA GACTCGATGA CCAGATCTTT 
ATCAACCGGA ATCCAGGAGT TCCTTCTGTT GTGAAGTTCC ATCCTTTTAC CTCAAGCATT 
GCTGTAGCAG ACAAGGACAG CATTTGTTTC TGGGACTGGG AGAAAGGAGA GAAGCTGGAT 
TACTTTCACA ATGGTAACCC TCGTTATACT CGTATAACAG CAGTCGAATA TCTGAATGGC 
CAAGATTGCT CTTTGCTGCT TACTGCTACA GATGATGGAG CCATCCGCGT GTGGAAGAAC 
TTTGCTGACT TAGAGAAAAA TCCAGAGATG GTGACAGCTT GGCAGGGACT GTCCGATATG 
CTGCCGACCA CACGAGGCCT GGCTCGAAGA GTTTCCATTT ATCTTGACAG GAGTAAACAG 
GGGGGGGCTG GCATGGTGGT GGATTGGGAG CAAGAAACAG GTCTACTCAT CACATCTGGA 
GACGTGCGAA TTATCCGGCT ATGGGACACA GACAGAGAGA CAAAAGTGCA GGATATACCC 
ACTGGAGCAG ACAGTTGTGT GACCAGCCTG TCATGTGACT CTCATCGTTC CCTTATTGTG 
GCTGGACTCG GAGATGGTTC AGTGCGAGTG TTTGATCGCA GGATGGCACT GAATGAATGC 
CGTGTAATGA CGTACCGAGA GCACTCAGCC TGGGTAGTAA AGACTTCTCT CCAAAAGCAG 
TCCGATGGGA ATATTGTGAG CGTCAGTGTA AATGGAGATA TCCGTTACTT CGACCCTCGT 
ATGCCAGAAT GTTTAAACTC ACTGCAGATT GTGAAGGGTC TTACTGCGTT GGATATCCAC 
CCACAGGCTA ACCTGTTTGC ATGTGGTTCA ATGAACCAGT TCATCGCTAT TTACAATGGA 
AATGGAGAGC TGATCAGCAA CATAAAGTAC TACGATGGAT TTATGGGACA AAGAATAGGA 
GCCATTAGCT GCCTGGCCTT TCATCCTTAC TGGCCCCACC TGGCCGTTGG CAGTAATGAC 
TATTATATGT CTATTTACTC AGTGGAGAAG CGAGCGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827007.1
CDS	123..4184
Translation	MELHTPALGLVGEDEETDLSDWNLPSAFMKKRHCEKIEGCKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLAMGPQKALETIGANLQKQYETWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPSHPLAQMPLPPSMKNCIQLAACDAAELLPMNPDLPADLFTSCLTTPIKIALRWFCMQKSVRLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCSPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTSFILAVIVNNYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDAARWCGVRDSAHEKLYSLLSDPMPEVRSSAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPMVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPASTEGGSLTPIRDSPGTPRLRSVSSYGNIRAVTTPRNLNKSLQNLSLSEESKTAGSAITFSPGNLSTSSSASSTLGSPDNEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDLAMKVLNSIAHKATVNARPQRILDASSLTQSAPASPTNKGMHIHQVGGSPPTPSASSSSLTNDVTKPPVSRDIPSNRPANAGGNVGVQYTPHSHQFPRTRKMFDKGPDQPGDDGDEAIVHKNFTVGSFQTGFCDWSAKYFAQPVMKMPEEHDMESQVRKEREWRFLRNTRVRKQAQRLIQKGISRLDDQIFINRNPGVPSVVKFHPFTSSIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRITAVEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGLARRVSIYLDRSKQGGAGMVVDWEQETGLLITSGDVRIIRLWDTDRETKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSVRVFDRRMALNECRVMTYREHSAWVVKTSLQKQSDGNIVSVSVNGDIRYFDPRMPECLNSLQIVKGLTALDIHPQANLFACGSMNQFIAIYNGNGELISNIKYYDGFMGQRIGAISCLAFHPYWPHLAVGSNDYYMSIYSVEKRAR

Target ORF information:

RefSeq Version	XM_012971553.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971553.2

ORF Insert Sequence:

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTAGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG AAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACA CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTT TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTCTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TCGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGAGTAA GACTGCTGGG AGTGCCATTA CATTTTCTCC TGGGAATCTA 
AGTACAAGTA GTAGCGCAAG CAGTACGTTA GGAAGCCCAG ATAATGAAGA GTACATCTTG 
TCCTTTGAGA CCATTGATAA GATGAGGCGT GTCAGTTCTT ACTCCTCTCT CAATTCCCTC 
ATAGGTGTCT CGTTCAATAG TGTCTACACT CAGATTTGGA GAGTGCTTCT CCACCTTGCT 
GCTGACCCAT ACCCTGAAGT CTCGGACCTT GCAATGAAAG TGCTAAACAG CATTGCTCAT 
AAGGCTACAG TGAATGCTCG CCCACAGCGG ATCCTCGATG CCTCCTCACT CACGCAGTCT 
GCTCCCGCCA GTCCCACCAA CAAAGGGATG CACATTCACC AAGTGGGTGG TTCCCCTCCG 
ACTCCCAGTG CCAGCAGTTC CAGTCTCACC AACGATGTAA CGAAACCACC TGTCAGTAGG 
GATATTCCCT CTAATCGACC TGCCAATGCT GGGGGCAATG TTGGGGTGCA GTACACGCCT 
CATTCCCACC AGTTTCCTCG TACAAGGAAA ATGTTCGACA AAGGACCAGA CCAGCCTGGA 
GATGATGGAG ATGAAGCCAT TGTACACAAG AACTTTACTG TAGGATCTTT TCAGACAGGC 
TTTTGTGATT GGAGTGCCAA ATATTTTGCC CAGCCAGTCA TGAAGATGCC AGAGGAACAT 
GACATGGAGA GTCAGGTACG TAAAGAACGA GAGTGGCGAT TTCTACGAAA TACAAGGGTA 
CGGAAACAGG CCCAGCGCCT TATACAGAAA GGAATCTCAA GACTCGATGA CCAGATCTTT 
ATCAACCGGA ATCCAGGAGT TCCTTCTGTT GTGAAGTTCC ATCCTTTTAC CTCAAGCATT 
GCTGTAGCAG ACAAGGACAG CATTTGTTTC TGGGACTGGG AGAAAGGAGA GAAGCTGGAT 
TACTTTCACA ATGGTAACCC TCGTTATACT CGTATAACAG CAGTCGAATA TCTGAATGGC 
CAAGATTGCT CTTTGCTGCT TACTGCTACA GATGATGGAG CCATCCGCGT GTGGAAGAAC 
TTTGCTGACT TAGAGAAAAA TCCAGAGATG GTGACAGCTT GGCAGGGACT GTCCGATATG 
CTGCCGACCA CACGAGGCCT GGCTCGAAGA GTTTCCATTT ATCTTGACAG GAGTAAACAG 
GGGGGGGCTG GCATGGTGGT GGATTGGGAG CAAGAAACAG GTCTACTCAT CACATCTGGA 
GACGTGCGAA TTATCCGGCT ATGGGACACA GACAGAGAGA CAAAAGTGCA GGATATACCC 
ACTGGAGCAG ACAGTTGTGT GACCAGCCTG TCATGTGACT CTCATCGTTC CCTTATTGTG 
GCTGGACTCG GAGATGGTTC AGTGCGAGTG TTTGATCGCA GGATGGCACT GAATGAATGC 
CGTGTAATGA CGTACCGAGA GCACTCAGCC TGGGTAGTAA AGACTTCTCT CCAAAAGCAG 
TCCGATGGGA ATATTGTGAG CGTCAGTGTA AATGGAGATA TCCGTTACTT CGACCCTCGT 
ATGCCAGAAT GTTTAAACTC ACTGCAGATT GTGAAGGGTC TTACTGCGTT GGATATCCAC 
CCACAGGCTA ACCTGTTTGC ATGTGGTTCA ATGAACCAGT TCATCGCTAT TTACAATGGA 
AATGGAGAGC TGATCAGCAA CATAAAGTAC TACGATGGAT TTATGGGACA AAGAATAGGA 
GCCATTAGCT GCCTGGCCTT TCATCCTTAC TGGCCCCACC TGGCCGTTGG CAGTAATGAC 
TATTATATGT CTATTTACTC AGTGGAGAAG CGAGCGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rptor ( XM_012971554.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827008.1 Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa32465
Clone ID Related Accession (Same CDS sequence)	XM_012971554.2
Accession Version	XM_012971554.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulatory-associated protein of mTOR isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971554.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTAGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG AAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACA CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTT TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTCTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TCGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGACTGG GAGTGCCATT ACATTTTCTC CTGGGAATCT AAGTACAAGT 
AGTAGCGCAA GCAGTACGTT AGGAAGCCCA GATAATGAAG AGTACATCTT GTCCTTTGAG 
ACCATTGATA AGATGAGGCG TGTCAGTTCT TACTCCTCTC TCAATTCCCT CATAGGTGTC 
TCGTTCAATA GTGTCTACAC TCAGATTTGG AGAGTGCTTC TCCACCTTGC TGCTGACCCA 
TACCCTGAAG TCTCGGACCT TGCAATGAAA GTGCTAAACA GCATTGCTCA TAAGGCTACA 
GTGAATGCTC GCCCACAGCG GATCCTCGAT GCCTCCTCAC TCACGCAGTC TGCTCCCGCC 
AGTCCCACCA ACAAAGGGAT GCACATTCAC CAAGTGGGTG GTTCCCCTCC GACTCCCAGT 
GCCAGCAGTT CCAGTCTCAC CAACGATGTA ACGAAACCAC CTGTCAGTAG GGATATTCCC 
TCTAATCGAC CTGCCAATGC TGGGGGCAAT GTTGGGGTGC AGTACACGCC TCATTCCCAC 
CAGTTTCCTC GTACAAGGAA AATGTTCGAC AAAGGACCAG ACCAGCCTGG AGATGATGGA 
GATGAAGCCA TTGTACACAA GAACTTTACT GTAGGATCTT TTCAGACAGG CTTTTGTGAT 
TGGAGTGCCA AATATTTTGC CCAGCCAGTC ATGAAGATGC CAGAGGAACA TGACATGGAG 
AGTCAGGTAC GTAAAGAACG AGAGTGGCGA TTTCTACGAA ATACAAGGGT ACGGAAACAG 
GCCCAGCGCC TTATACAGAA AGGAATCTCA AGACTCGATG ACCAGATCTT TATCAACCGG 
AATCCAGGAG TTCCTTCTGT TGTGAAGTTC CATCCTTTTA CCTCAAGCAT TGCTGTAGCA 
GACAAGGACA GCATTTGTTT CTGGGACTGG GAGAAAGGAG AGAAGCTGGA TTACTTTCAC 
AATGGTAACC CTCGTTATAC TCGTATAACA GCAGTCGAAT ATCTGAATGG CCAAGATTGC 
TCTTTGCTGC TTACTGCTAC AGATGATGGA GCCATCCGCG TGTGGAAGAA CTTTGCTGAC 
TTAGAGAAAA ATCCAGAGAT GGTGACAGCT TGGCAGGGAC TGTCCGATAT GCTGCCGACC 
ACACGAGGCC TGGCTCGAAG AGTTTCCATT TATCTTGACA GGAGTAAACA GGGGGGGGCT 
GGCATGGTGG TGGATTGGGA GCAAGAAACA GGTCTACTCA TCACATCTGG AGACGTGCGA 
ATTATCCGGC TATGGGACAC AGACAGAGAG ACAAAAGTGC AGGATATACC CACTGGAGCA 
GACAGTTGTG TGACCAGCCT GTCATGTGAC TCTCATCGTT CCCTTATTGT GGCTGGACTC 
GGAGATGGTT CAGTGCGAGT GTTTGATCGC AGGATGGCAC TGAATGAATG CCGTGTAATG 
ACGTACCGAG AGCACTCAGC CTGGGTAGTA AAGACTTCTC TCCAAAAGCA GTCCGATGGG 
AATATTGTGA GCGTCAGTGT AAATGGAGAT ATCCGTTACT TCGACCCTCG TATGCCAGAA 
TGTTTAAACT CACTGCAGAT TGTGAAGGGT CTTACTGCGT TGGATATCCA CCCACAGGCT 
AACCTGTTTG CATGTGGTTC AATGAACCAG TTCATCGCTA TTTACAATGG AAATGGAGAG 
CTGATCAGCA ACATAAAGTA CTACGATGGA TTTATGGGAC AAAGAATAGG AGCCATTAGC 
TGCCTGGCCT TTCATCCTTA CTGGCCCCAC CTGGCCGTTG GCAGTAATGA CTATTATATG 
TCTATTTACT CAGTGGAGAA GCGAGCGAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827008.1
CDS	123..4175
Translation	MELHTPALGLVGEDEETDLSDWNLPSAFMKKRHCEKIEGCKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLAMGPQKALETIGANLQKQYETWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPSHPLAQMPLPPSMKNCIQLAACDAAELLPMNPDLPADLFTSCLTTPIKIALRWFCMQKSVRLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCSPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTSFILAVIVNNYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDAARWCGVRDSAHEKLYSLLSDPMPEVRSSAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPMVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPASTEGGSLTPIRDSPGTPRLRSVSSYGNIRAVTTPRNLNKSLQNLSLSEETGSAITFSPGNLSTSSSASSTLGSPDNEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDLAMKVLNSIAHKATVNARPQRILDASSLTQSAPASPTNKGMHIHQVGGSPPTPSASSSSLTNDVTKPPVSRDIPSNRPANAGGNVGVQYTPHSHQFPRTRKMFDKGPDQPGDDGDEAIVHKNFTVGSFQTGFCDWSAKYFAQPVMKMPEEHDMESQVRKEREWRFLRNTRVRKQAQRLIQKGISRLDDQIFINRNPGVPSVVKFHPFTSSIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRITAVEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGLARRVSIYLDRSKQGGAGMVVDWEQETGLLITSGDVRIIRLWDTDRETKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSVRVFDRRMALNECRVMTYREHSAWVVKTSLQKQSDGNIVSVSVNGDIRYFDPRMPECLNSLQIVKGLTALDIHPQANLFACGSMNQFIAIYNGNGELISNIKYYDGFMGQRIGAISCLAFHPYWPHLAVGSNDYYMSIYSVEKRAR

Target ORF information:

RefSeq Version	XM_012971554.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971554.2

ORF Insert Sequence:

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTAGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG AAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACA CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTT TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTCTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TCGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGACTGG GAGTGCCATT ACATTTTCTC CTGGGAATCT AAGTACAAGT 
AGTAGCGCAA GCAGTACGTT AGGAAGCCCA GATAATGAAG AGTACATCTT GTCCTTTGAG 
ACCATTGATA AGATGAGGCG TGTCAGTTCT TACTCCTCTC TCAATTCCCT CATAGGTGTC 
TCGTTCAATA GTGTCTACAC TCAGATTTGG AGAGTGCTTC TCCACCTTGC TGCTGACCCA 
TACCCTGAAG TCTCGGACCT TGCAATGAAA GTGCTAAACA GCATTGCTCA TAAGGCTACA 
GTGAATGCTC GCCCACAGCG GATCCTCGAT GCCTCCTCAC TCACGCAGTC TGCTCCCGCC 
AGTCCCACCA ACAAAGGGAT GCACATTCAC CAAGTGGGTG GTTCCCCTCC GACTCCCAGT 
GCCAGCAGTT CCAGTCTCAC CAACGATGTA ACGAAACCAC CTGTCAGTAG GGATATTCCC 
TCTAATCGAC CTGCCAATGC TGGGGGCAAT GTTGGGGTGC AGTACACGCC TCATTCCCAC 
CAGTTTCCTC GTACAAGGAA AATGTTCGAC AAAGGACCAG ACCAGCCTGG AGATGATGGA 
GATGAAGCCA TTGTACACAA GAACTTTACT GTAGGATCTT TTCAGACAGG CTTTTGTGAT 
TGGAGTGCCA AATATTTTGC CCAGCCAGTC ATGAAGATGC CAGAGGAACA TGACATGGAG 
AGTCAGGTAC GTAAAGAACG AGAGTGGCGA TTTCTACGAA ATACAAGGGT ACGGAAACAG 
GCCCAGCGCC TTATACAGAA AGGAATCTCA AGACTCGATG ACCAGATCTT TATCAACCGG 
AATCCAGGAG TTCCTTCTGT TGTGAAGTTC CATCCTTTTA CCTCAAGCAT TGCTGTAGCA 
GACAAGGACA GCATTTGTTT CTGGGACTGG GAGAAAGGAG AGAAGCTGGA TTACTTTCAC 
AATGGTAACC CTCGTTATAC TCGTATAACA GCAGTCGAAT ATCTGAATGG CCAAGATTGC 
TCTTTGCTGC TTACTGCTAC AGATGATGGA GCCATCCGCG TGTGGAAGAA CTTTGCTGAC 
TTAGAGAAAA ATCCAGAGAT GGTGACAGCT TGGCAGGGAC TGTCCGATAT GCTGCCGACC 
ACACGAGGCC TGGCTCGAAG AGTTTCCATT TATCTTGACA GGAGTAAACA GGGGGGGGCT 
GGCATGGTGG TGGATTGGGA GCAAGAAACA GGTCTACTCA TCACATCTGG AGACGTGCGA 
ATTATCCGGC TATGGGACAC AGACAGAGAG ACAAAAGTGC AGGATATACC CACTGGAGCA 
GACAGTTGTG TGACCAGCCT GTCATGTGAC TCTCATCGTT CCCTTATTGT GGCTGGACTC 
GGAGATGGTT CAGTGCGAGT GTTTGATCGC AGGATGGCAC TGAATGAATG CCGTGTAATG 
ACGTACCGAG AGCACTCAGC CTGGGTAGTA AAGACTTCTC TCCAAAAGCA GTCCGATGGG 
AATATTGTGA GCGTCAGTGT AAATGGAGAT ATCCGTTACT TCGACCCTCG TATGCCAGAA 
TGTTTAAACT CACTGCAGAT TGTGAAGGGT CTTACTGCGT TGGATATCCA CCCACAGGCT 
AACCTGTTTG CATGTGGTTC AATGAACCAG TTCATCGCTA TTTACAATGG AAATGGAGAG 
CTGATCAGCA ACATAAAGTA CTACGATGGA TTTATGGGAC AAAGAATAGG AGCCATTAGC 
TGCCTGGCCT TTCATCCTTA CTGGCCCCAC CTGGCCGTTG GCAGTAATGA CTATTATATG 
TCTATTTACT CAGTGGAGAA GCGAGCGAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rptor ( XM_012971555.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827009.1 Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X3, mRNA.

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CloneID	OXa32466
Clone ID Related Accession (Same CDS sequence)	XM_012971555.2
Accession Version	XM_012971555.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulatory-associated protein of mTOR isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971555.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTAGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG AAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACA CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTT TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTCTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TCGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGAGTAA GACTGCTGGG AGTGCCATTA CATTTTCTCC TGGGAATCTA 
AGTACAAGTA GTAGCGCAAG CAGTACGTTA GGAAGCCCAG ATAATGAAGA GTACATCTTG 
TCCTTTGAGA CCATTGATAA GATGAGGCGT GTCAGTTCTT ACTCCTCTCT CAATTCCCTC 
ATAGGTGTCT CGTTCAATAG TGTCTACACT CAGATTTGGA GAGTGCTTCT CCACCTTGCT 
GCTGACCCAT ACCCTGAAGT CTCGGACCTT GCAATGAAAG TGCTAAACAG CATTGCTCAT 
AAGGCTACAG TGAATGCTCG CCCACAGCGG ATCCTCGATG CCTCCTCACT CACGCAGTCT 
GCTCCCGCCA GTCCCACCAA CAAAGGGATG CACATTCACC AAGTGGGTGG TTCCCCTCCG 
ACTCCCAGTG CCAGCAGTTC CAGTCTCACC AACGATGTAA CGAAACCACC TGTCAGTAGG 
GATATTCCCT CTAATCGACC TGCCAATGCT GGGGGCAATG TTGGGGTGCA GTACACGCCT 
CATTCCCACC AGTTTCCTCG TACAAGGAAA ATGTTCGACA AAGGACCAGA CCAGCCTGGA 
GATGATGGAG ATGAAGCCAT TGTACACAAG AACTTTACTG TAGGATCTTT TCAGACAGGC 
TTTTGTGATT GGAGTGCCAA ATATTTTGCC CAGCCAGTCA TGAAGATGCC AGAGGAACAT 
GACATGGAGA GTCAGGTACG TAAAGAACGA GAGTGGCGAT TTCTACGAAA TACAAGGGTA 
CGGAAACAGG CCCAGCGCCT TATACAGAAA GGAATCTCAA GACTCGATGA CCAGATCTTT 
ATCAACCGGA ATCCAGGAGT TCCTTCTGTT GTGAAGTTCC ATCCTTTTAC CTCAAGCATT 
GCTGTAGCAG ACAAGGACAG CATTTGTTTC TGGGACTGGG AGAAAGGAGA GAAGCTGGAT 
TACTTTCACA ATGGTAACCC TCGTTATACT CGTATAACAG CAGTCGAATA TCTGAATGGC 
CAAGATTGCT CTTTGCTGCT TACTGCTACA GATGATGGAG CCATCCGCGT GTGGAAGAAC 
TTTGCTGACT TAGAGAAAAA TCCAGAGATG GTGACAGCTT GGCAGGGACT GTCCGATATG 
CTGCCGACCA CACGAGGGGC TGGCATGGTG GTGGATTGGG AGCAAGAAAC AGGTCTACTC 
ATCACATCTG GAGACGTGCG AATTATCCGG CTATGGGACA CAGACAGAGA GACAAAAGTG 
CAGGATATAC CCACTGGAGC AGACAGTTGT GTGACCAGCC TGTCATGTGA CTCTCATCGT 
TCCCTTATTG TGGCTGGACT CGGAGATGGT TCAGTGCGAG TGTTTGATCG CAGGATGGCA 
CTGAATGAAT GCCGTGTAAT GACGTACCGA GAGCACTCAG CCTGGGTAGT AAAGACTTCT 
CTCCAAAAGC AGTCCGATGG GAATATTGTG AGCGTCAGTG TAAATGGAGA TATCCGTTAC 
TTCGACCCTC GTATGCCAGA ATGTTTAAAC TCACTGCAGA TTGTGAAGGG TCTTACTGCG 
TTGGATATCC ACCCACAGGC TAACCTGTTT GCATGTGGTT CAATGAACCA GTTCATCGCT 
ATTTACAATG GAAATGGAGA GCTGATCAGC AACATAAAGT ACTACGATGG ATTTATGGGA 
CAAAGAATAG GAGCCATTAG CTGCCTGGCC TTTCATCCTT ACTGGCCCCA CCTGGCCGTT 
GGCAGTAATG ACTATTATAT GTCTATTTAC TCAGTGGAGA AGCGAGCGAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827009.1
CDS	123..4136
Translation	MELHTPALGLVGEDEETDLSDWNLPSAFMKKRHCEKIEGCKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLAMGPQKALETIGANLQKQYETWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPSHPLAQMPLPPSMKNCIQLAACDAAELLPMNPDLPADLFTSCLTTPIKIALRWFCMQKSVRLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCSPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTSFILAVIVNNYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDAARWCGVRDSAHEKLYSLLSDPMPEVRSSAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPMVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPASTEGGSLTPIRDSPGTPRLRSVSSYGNIRAVTTPRNLNKSLQNLSLSEESKTAGSAITFSPGNLSTSSSASSTLGSPDNEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDLAMKVLNSIAHKATVNARPQRILDASSLTQSAPASPTNKGMHIHQVGGSPPTPSASSSSLTNDVTKPPVSRDIPSNRPANAGGNVGVQYTPHSHQFPRTRKMFDKGPDQPGDDGDEAIVHKNFTVGSFQTGFCDWSAKYFAQPVMKMPEEHDMESQVRKEREWRFLRNTRVRKQAQRLIQKGISRLDDQIFINRNPGVPSVVKFHPFTSSIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRITAVEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLITSGDVRIIRLWDTDRETKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSVRVFDRRMALNECRVMTYREHSAWVVKTSLQKQSDGNIVSVSVNGDIRYFDPRMPECLNSLQIVKGLTALDIHPQANLFACGSMNQFIAIYNGNGELISNIKYYDGFMGQRIGAISCLAFHPYWPHLAVGSNDYYMSIYSVEKRAR

Target ORF information:

RefSeq Version	XM_012971555.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971555.2

ORF Insert Sequence:

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTAGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG AAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACA CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTT TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTCTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TCGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGAGTAA GACTGCTGGG AGTGCCATTA CATTTTCTCC TGGGAATCTA 
AGTACAAGTA GTAGCGCAAG CAGTACGTTA GGAAGCCCAG ATAATGAAGA GTACATCTTG 
TCCTTTGAGA CCATTGATAA GATGAGGCGT GTCAGTTCTT ACTCCTCTCT CAATTCCCTC 
ATAGGTGTCT CGTTCAATAG TGTCTACACT CAGATTTGGA GAGTGCTTCT CCACCTTGCT 
GCTGACCCAT ACCCTGAAGT CTCGGACCTT GCAATGAAAG TGCTAAACAG CATTGCTCAT 
AAGGCTACAG TGAATGCTCG CCCACAGCGG ATCCTCGATG CCTCCTCACT CACGCAGTCT 
GCTCCCGCCA GTCCCACCAA CAAAGGGATG CACATTCACC AAGTGGGTGG TTCCCCTCCG 
ACTCCCAGTG CCAGCAGTTC CAGTCTCACC AACGATGTAA CGAAACCACC TGTCAGTAGG 
GATATTCCCT CTAATCGACC TGCCAATGCT GGGGGCAATG TTGGGGTGCA GTACACGCCT 
CATTCCCACC AGTTTCCTCG TACAAGGAAA ATGTTCGACA AAGGACCAGA CCAGCCTGGA 
GATGATGGAG ATGAAGCCAT TGTACACAAG AACTTTACTG TAGGATCTTT TCAGACAGGC 
TTTTGTGATT GGAGTGCCAA ATATTTTGCC CAGCCAGTCA TGAAGATGCC AGAGGAACAT 
GACATGGAGA GTCAGGTACG TAAAGAACGA GAGTGGCGAT TTCTACGAAA TACAAGGGTA 
CGGAAACAGG CCCAGCGCCT TATACAGAAA GGAATCTCAA GACTCGATGA CCAGATCTTT 
ATCAACCGGA ATCCAGGAGT TCCTTCTGTT GTGAAGTTCC ATCCTTTTAC CTCAAGCATT 
GCTGTAGCAG ACAAGGACAG CATTTGTTTC TGGGACTGGG AGAAAGGAGA GAAGCTGGAT 
TACTTTCACA ATGGTAACCC TCGTTATACT CGTATAACAG CAGTCGAATA TCTGAATGGC 
CAAGATTGCT CTTTGCTGCT TACTGCTACA GATGATGGAG CCATCCGCGT GTGGAAGAAC 
TTTGCTGACT TAGAGAAAAA TCCAGAGATG GTGACAGCTT GGCAGGGACT GTCCGATATG 
CTGCCGACCA CACGAGGGGC TGGCATGGTG GTGGATTGGG AGCAAGAAAC AGGTCTACTC 
ATCACATCTG GAGACGTGCG AATTATCCGG CTATGGGACA CAGACAGAGA GACAAAAGTG 
CAGGATATAC CCACTGGAGC AGACAGTTGT GTGACCAGCC TGTCATGTGA CTCTCATCGT 
TCCCTTATTG TGGCTGGACT CGGAGATGGT TCAGTGCGAG TGTTTGATCG CAGGATGGCA 
CTGAATGAAT GCCGTGTAAT GACGTACCGA GAGCACTCAG CCTGGGTAGT AAAGACTTCT 
CTCCAAAAGC AGTCCGATGG GAATATTGTG AGCGTCAGTG TAAATGGAGA TATCCGTTAC 
TTCGACCCTC GTATGCCAGA ATGTTTAAAC TCACTGCAGA TTGTGAAGGG TCTTACTGCG 
TTGGATATCC ACCCACAGGC TAACCTGTTT GCATGTGGTT CAATGAACCA GTTCATCGCT 
ATTTACAATG GAAATGGAGA GCTGATCAGC AACATAAAGT ACTACGATGG ATTTATGGGA 
CAAAGAATAG GAGCCATTAG CTGCCTGGCC TTTCATCCTT ACTGGCCCCA CCTGGCCGTT 
GGCAGTAATG ACTATTATAT GTCTATTTAC TCAGTGGAGA AGCGAGCGAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rptor ( XM_012971556.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012827010.1 Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X4, mRNA.

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CloneID	OXa32467
Clone ID Related Accession (Same CDS sequence)	XM_012971556.2
Accession Version	XM_012971556.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulatory-associated protein of mTOR isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971556.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTAGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG AAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACA CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTT TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTCTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TCGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGACTGG GAGTGCCATT ACATTTTCTC CTGGGAATCT AAGTACAAGT 
AGTAGCGCAA GCAGTACGTT AGGAAGCCCA GATAATGAAG AGTACATCTT GTCCTTTGAG 
ACCATTGATA AGATGAGGCG TGTCAGTTCT TACTCCTCTC TCAATTCCCT CATAGGTGTC 
TCGTTCAATA GTGTCTACAC TCAGATTTGG AGAGTGCTTC TCCACCTTGC TGCTGACCCA 
TACCCTGAAG TCTCGGACCT TGCAATGAAA GTGCTAAACA GCATTGCTCA TAAGGCTACA 
GTGAATGCTC GCCCACAGCG GATCCTCGAT GCCTCCTCAC TCACGCAGTC TGCTCCCGCC 
AGTCCCACCA ACAAAGGGAT GCACATTCAC CAAGTGGGTG GTTCCCCTCC GACTCCCAGT 
GCCAGCAGTT CCAGTCTCAC CAACGATGTA ACGAAACCAC CTGTCAGTAG GGATATTCCC 
TCTAATCGAC CTGCCAATGC TGGGGGCAAT GTTGGGGTGC AGTACACGCC TCATTCCCAC 
CAGTTTCCTC GTACAAGGAA AATGTTCGAC AAAGGACCAG ACCAGCCTGG AGATGATGGA 
GATGAAGCCA TTGTACACAA GAACTTTACT GTAGGATCTT TTCAGACAGG CTTTTGTGAT 
TGGAGTGCCA AATATTTTGC CCAGCCAGTC ATGAAGATGC CAGAGGAACA TGACATGGAG 
AGTCAGGTAC GTAAAGAACG AGAGTGGCGA TTTCTACGAA ATACAAGGGT ACGGAAACAG 
GCCCAGCGCC TTATACAGAA AGGAATCTCA AGACTCGATG ACCAGATCTT TATCAACCGG 
AATCCAGGAG TTCCTTCTGT TGTGAAGTTC CATCCTTTTA CCTCAAGCAT TGCTGTAGCA 
GACAAGGACA GCATTTGTTT CTGGGACTGG GAGAAAGGAG AGAAGCTGGA TTACTTTCAC 
AATGGTAACC CTCGTTATAC TCGTATAACA GCAGTCGAAT ATCTGAATGG CCAAGATTGC 
TCTTTGCTGC TTACTGCTAC AGATGATGGA GCCATCCGCG TGTGGAAGAA CTTTGCTGAC 
TTAGAGAAAA ATCCAGAGAT GGTGACAGCT TGGCAGGGAC TGTCCGATAT GCTGCCGACC 
ACACGAGGGG CTGGCATGGT GGTGGATTGG GAGCAAGAAA CAGGTCTACT CATCACATCT 
GGAGACGTGC GAATTATCCG GCTATGGGAC ACAGACAGAG AGACAAAAGT GCAGGATATA 
CCCACTGGAG CAGACAGTTG TGTGACCAGC CTGTCATGTG ACTCTCATCG TTCCCTTATT 
GTGGCTGGAC TCGGAGATGG TTCAGTGCGA GTGTTTGATC GCAGGATGGC ACTGAATGAA 
TGCCGTGTAA TGACGTACCG AGAGCACTCA GCCTGGGTAG TAAAGACTTC TCTCCAAAAG 
CAGTCCGATG GGAATATTGT GAGCGTCAGT GTAAATGGAG ATATCCGTTA CTTCGACCCT 
CGTATGCCAG AATGTTTAAA CTCACTGCAG ATTGTGAAGG GTCTTACTGC GTTGGATATC 
CACCCACAGG CTAACCTGTT TGCATGTGGT TCAATGAACC AGTTCATCGC TATTTACAAT 
GGAAATGGAG AGCTGATCAG CAACATAAAG TACTACGATG GATTTATGGG ACAAAGAATA 
GGAGCCATTA GCTGCCTGGC CTTTCATCCT TACTGGCCCC ACCTGGCCGT TGGCAGTAAT 
GACTATTATA TGTCTATTTA CTCAGTGGAG AAGCGAGCGA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012827010.1
CDS	123..4127
Translation	MELHTPALGLVGEDEETDLSDWNLPSAFMKKRHCEKIEGCKSLAQSWRMKDRMKTVSVALVLCLNVGVDPPDVVKTTPCARLECWIDPLAMGPQKALETIGANLQKQYETWQPRARYKQSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEIWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPSHPLAQMPLPPSMKNCIQLAACDAAELLPMNPDLPADLFTSCLTTPIKIALRWFCMQKSVRLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSYNCSPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWLTMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTSFILAVIVNNYNTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDAARWCGVRDSAHEKLYSLLSDPMPEVRSSAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLINDGSPMVRKELVVALSHLVVQYESNFCTVALQFMEEEKNYPLPSPASTEGGSLTPIRDSPGTPRLRSVSSYGNIRAVTTPRNLNKSLQNLSLSEETGSAITFSPGNLSTSSSASSTLGSPDNEEYILSFETIDKMRRVSSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDLAMKVLNSIAHKATVNARPQRILDASSLTQSAPASPTNKGMHIHQVGGSPPTPSASSSSLTNDVTKPPVSRDIPSNRPANAGGNVGVQYTPHSHQFPRTRKMFDKGPDQPGDDGDEAIVHKNFTVGSFQTGFCDWSAKYFAQPVMKMPEEHDMESQVRKEREWRFLRNTRVRKQAQRLIQKGISRLDDQIFINRNPGVPSVVKFHPFTSSIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRITAVEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLITSGDVRIIRLWDTDRETKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSVRVFDRRMALNECRVMTYREHSAWVVKTSLQKQSDGNIVSVSVNGDIRYFDPRMPECLNSLQIVKGLTALDIHPQANLFACGSMNQFIAIYNGNGELISNIKYYDGFMGQRIGAISCLAFHPYWPHLAVGSNDYYMSIYSVEKRAR

Target ORF information:

RefSeq Version	XM_012971556.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis regulatory associated protein of MTOR, complex 1 (rptor), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971556.2

ORF Insert Sequence:

ATGGAGCTGC ACACCCCGGC CCTGGGACTA GTCGGGGAGG ATGAAGAAAC CGATTTGAGC 
GATTGGAATT TGCCGTCTGC GTTCATGAAG AAGCGGCATT GTGAGAAGAT CGAAGGCTGC 
AAGTCACTTG CACAGAGTTG GAGGATGAAA GATCGGATGA AGACAGTGAG TGTGGCTCTG 
GTGCTGTGCT TAAATGTAGG AGTGGATCCC CCAGATGTAG TTAAGACCAC GCCATGTGCT 
CGCTTGGAGT GCTGGATTGA TCCACTGGCT ATGGGTCCAC AGAAAGCCCT GGAAACCATT 
GGGGCAAACT TACAAAAGCA GTATGAAACC TGGCAACCAC GGGCTCGGTA CAAACAGAGT 
CTAGATCCAA CTGTGGATGA GGTTAAAAAA CTCTGCACTT CGCTGAGAAG AAATGCCAAG 
GAGGAAAGGG TGCTGTTCCA TTATAATGGA CATGGGGTTC CCCGACCTAC AGTCAACGGG 
GAAATCTGGG TCTTTAATAA GAATTACACA CAGTATATTC CTCTTTCTAT ATATGACCTA 
CAGACCTGGA TGGGAAGCCC ATCTATCTTT GTATATGACT GCTCAAATGC CGGCCTCATT 
GTCAAGTCTT TCAAGCAATT TGCACTACAG AGAGAGCAAG AGCTTGAGGT TGCTGCTATT 
AACCCAAGCC ACCCATTAGC ACAGATGCCA TTGCCACCAT CAATGAAGAA TTGCATCCAG 
TTGGCAGCTT GTGATGCAGC TGAACTTTTG CCTATGAACC CCGACCTTCC TGCTGACCTT 
TTCACTTCTT GCTTGACCAC ACCTATTAAG ATTGCCCTGA GATGGTTTTG TATGCAGAAA 
AGTGTGCGAT TGGTGCCTGG TGTTACTCTG GATCTTATAG AGAAAATTCC TGGGCGATTA 
AATGATAGGA GGACACCACT GGGAGAGCTG AACTGGATCT TTACAGCAAT CACAGATACT 
ATAGCATGGA ACGTTCTGCC TAGAGACCTA TTTCAGAAGC TCTTTAGACA GGACCTATTA 
GTTGCCAGTC TCTTCCGGAA CTTTCTTCTG GCCGAGAGGA TCATGAGATC ATACAATTGC 
AGCCCAGTGA GCAGCCCTCG TTTGCCACCC ACCTACATGC ATGCTATGTG GCAGGCATGG 
GATCTTGCTG TCGATATATG TCTTTCTCAG CTACCCACCA TTATTGAGGA GGGAACTGCT 
TTCCGGCACA GCCCTTTCTT TGCTGAGCAG CTGACTGCAT TTCAGGTGTG GCTGACTATG 
GGTGTTGAGA ACAGAAACCC CCCAGAACAG TTGCCCATTG TGCTGCAGGT TTTGCTGAGC 
CAGGTTCATA GACTCCGTGC CTTGGACCTT TTGGGAAGAT TCTTGGATCT GGGTCCCTGG 
GCTGTCAGTC TGGCCCTCTC TGTTGGTATT TTCCCTTATG TGCTAAAGCT GCTCCAAAGC 
TCAGCTCGGG AACTCCGGCC ACTGCTTGTC TTTATTTGGG CCAAGATCCT TGCTGTTGAT 
AGTTCGTGCC AAGCAGACTT AGTAAAAGAT AATGGCCATA AATATTTTCT TTCTGTTCTG 
GCGGATCCAT ATATGCCGGC TGAGCACCGT ACAATGACCT CCTTCATTTT AGCAGTAATT 
GTTAACAATT ATAACACTGG CCAGGAAGCC TGCCTCCAAG GAAACCTGAT CGCTATCTGC 
TTGGAGCAGC TAAATGATCC ACACCCTCTC CTACGCCAAT GGGTTGCTAT CTGCCTGGGA 
CGCATCTGGC AGAATTTTGA TGCTGCACGT TGGTGTGGGG TACGTGATAG CGCCCATGAG 
AAGCTGTACA GCCTCTTGTC TGACCCCATG CCAGAGGTCC GTAGTTCTGC AGTTTTTGCC 
CTTGGCACCT TCGTTGGGAA TTCAGCTGAG AGAACAGATC ACTCTACAAC AATAGATCAC 
AATGTTGCAA TGATGTTAGC ACAGCTTATC AATGATGGGA GTCCTATGGT GCGCAAGGAG 
CTTGTTGTAG CTCTTAGTCA TCTGGTTGTG CAGTATGAAA GTAACTTCTG TACAGTTGCC 
CTCCAGTTTA TGGAGGAAGA GAAGAATTAC CCCCTGCCCT CGCCAGCTTC CACAGAGGGA 
GGCAGTTTGA CACCAATACG AGATAGCCCT GGAACGCCCC GCTTGAGATC TGTGAGCTCC 
TACGGTAATA TCCGGGCAGT GACAACTCCA AGAAACTTGA ACAAGTCTCT GCAGAATCTA 
AGCCTGAGTG AAGAGACTGG GAGTGCCATT ACATTTTCTC CTGGGAATCT AAGTACAAGT 
AGTAGCGCAA GCAGTACGTT AGGAAGCCCA GATAATGAAG AGTACATCTT GTCCTTTGAG 
ACCATTGATA AGATGAGGCG TGTCAGTTCT TACTCCTCTC TCAATTCCCT CATAGGTGTC 
TCGTTCAATA GTGTCTACAC TCAGATTTGG AGAGTGCTTC TCCACCTTGC TGCTGACCCA 
TACCCTGAAG TCTCGGACCT TGCAATGAAA GTGCTAAACA GCATTGCTCA TAAGGCTACA 
GTGAATGCTC GCCCACAGCG GATCCTCGAT GCCTCCTCAC TCACGCAGTC TGCTCCCGCC 
AGTCCCACCA ACAAAGGGAT GCACATTCAC CAAGTGGGTG GTTCCCCTCC GACTCCCAGT 
GCCAGCAGTT CCAGTCTCAC CAACGATGTA ACGAAACCAC CTGTCAGTAG GGATATTCCC 
TCTAATCGAC CTGCCAATGC TGGGGGCAAT GTTGGGGTGC AGTACACGCC TCATTCCCAC 
CAGTTTCCTC GTACAAGGAA AATGTTCGAC AAAGGACCAG ACCAGCCTGG AGATGATGGA 
GATGAAGCCA TTGTACACAA GAACTTTACT GTAGGATCTT TTCAGACAGG CTTTTGTGAT 
TGGAGTGCCA AATATTTTGC CCAGCCAGTC ATGAAGATGC CAGAGGAACA TGACATGGAG 
AGTCAGGTAC GTAAAGAACG AGAGTGGCGA TTTCTACGAA ATACAAGGGT ACGGAAACAG 
GCCCAGCGCC TTATACAGAA AGGAATCTCA AGACTCGATG ACCAGATCTT TATCAACCGG 
AATCCAGGAG TTCCTTCTGT TGTGAAGTTC CATCCTTTTA CCTCAAGCAT TGCTGTAGCA 
GACAAGGACA GCATTTGTTT CTGGGACTGG GAGAAAGGAG AGAAGCTGGA TTACTTTCAC 
AATGGTAACC CTCGTTATAC TCGTATAACA GCAGTCGAAT ATCTGAATGG CCAAGATTGC 
TCTTTGCTGC TTACTGCTAC AGATGATGGA GCCATCCGCG TGTGGAAGAA CTTTGCTGAC 
TTAGAGAAAA ATCCAGAGAT GGTGACAGCT TGGCAGGGAC TGTCCGATAT GCTGCCGACC 
ACACGAGGGG CTGGCATGGT GGTGGATTGG GAGCAAGAAA CAGGTCTACT CATCACATCT 
GGAGACGTGC GAATTATCCG GCTATGGGAC ACAGACAGAG AGACAAAAGT GCAGGATATA 
CCCACTGGAG CAGACAGTTG TGTGACCAGC CTGTCATGTG ACTCTCATCG TTCCCTTATT 
GTGGCTGGAC TCGGAGATGG TTCAGTGCGA GTGTTTGATC GCAGGATGGC ACTGAATGAA 
TGCCGTGTAA TGACGTACCG AGAGCACTCA GCCTGGGTAG TAAAGACTTC TCTCCAAAAG 
CAGTCCGATG GGAATATTGT GAGCGTCAGT GTAAATGGAG ATATCCGTTA CTTCGACCCT 
CGTATGCCAG AATGTTTAAA CTCACTGCAG ATTGTGAAGG GTCTTACTGC GTTGGATATC 
CACCCACAGG CTAACCTGTT TGCATGTGGT TCAATGAACC AGTTCATCGC TATTTACAAT 
GGAAATGGAG AGCTGATCAG CAACATAAAG TACTACGATG GATTTATGGG ACAAAGAATA 
GGAGCCATTA GCTGCCTGGC CTTTCATCCT TACTGGCCCC ACCTGGCCGT TGGCAGTAAT 
GACTATTATA TGTCTATTTA CTCAGTGGAG AAGCGAGCGA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	rptor
Entrez Gene ID	100170605
Full Name	regulatory associated protein of MTOR, complex 1
Synonyms	raptor
General protein information	Preferred Names regulatory associated protein of MTOR, complex 1 Names regulatory-associated protein of mTOR
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Mus musculus (house mouse)	Rptor	NP_083174.2
Rattus norvegicus (Norway rat)	Rptor	NP_001127971.2
Homo sapiens (human)	RPTOR	NP_065812.1
Canis lupus familiaris (dog)	RPTOR	XP_850487.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010035	XP_001689178.1
Kluyveromyces lactis	KLLA0A09471g	XP_451414.1
Magnaporthe oryzae (rice blast fungus)	MGG_02874	XP_003720858.1
Gallus gallus (chicken)	RPTOR	XP_426232.2
Drosophila melanogaster (fruit fly)	raptor	NP_572294.2
Saccharomyces cerevisiae (baker's yeast)	KOG1	NP_012056.1
Schizosaccharomyces pombe (fission yeast)	mip1	NP_593370.1
	RPTOR	XP_001110471.1
Neurospora crassa	NCU00621	XP_965761.1
Arabidopsis thaliana (thale cress)	RAPTOR2	NP_195797.3
Pan troglodytes (chimpanzee)	RPTOR	XP_003953266.1
Bos taurus (cattle)	RPTOR	NP_001179059.1
Danio rerio (zebrafish)	rptor	XP_005157411.1
Eremothecium gossypii	AGOS_ADR331C	NP_984427.2
Arabidopsis thaliana (thale cress)	RAPTOR1	NP_566335.1
Oryza sativa (rice)	Os12g0110000	NP_001065961.1
Xenopus tropicalis (tropical clawed frog)	rptor	NP_001123843.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

rptor cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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