chs-5 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	chs-5
Entrez Gene ID	3872480
Full Name	chitin synthase-5
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_951240.3	XP_956333.2	chitin synthase C

Pathways from BioSystems

Homologies

Neurospora crassa	NCU04352	XP_956333.2
Magnaporthe oryzae (rice blast fungus)	MGG_13013	XP_003712159.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chs-5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chs-5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf05414	XM_951240.3 Latest version!	Neurospora crassa OR74A chitin synthase C (chs-5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chs-5 ( XM_951240.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_956333.2 Neurospora crassa OR74A chitin synthase C (chs-5), mRNA.

Price & Availability↑

CloneID	ONf05414
Clone ID Related Accession (Same CDS sequence)	XM_951240.3
Accession Version	XM_951240.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	chitin synthase C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_951240.2.

ATGGCGCTCA ACCTTCCTCC CGTAGGGGGG ACGGGTGGAG GGGCCCATAC CCAACCTTCG 
TTACCTTCGC TGCCAACCCA TCTTCAGTCG GATACACACT TGACGGCTCA CCTGGCCAGT 
CGATTCCACG TCTCGCTACC GATTTCCCGA CTTTCATCCC ACGCACTCGT TTCCATCAAC 
ACATACACTA GCTCCACGAA AGGAGATGGC ACGCGAGAGT CTTCCGCCAT GGGCGGTGCC 
GAGGACATGG CCGAGAGGGC CTACATCCGT CTCGGTCATC GGTCAGAGAA CCAGGCCGTG 
CTCTTCTTGG GTGAATCGGG CTCGGGAAAG ACGACCATTC GTGCGCATGT CCTGACCGCA 
CTCCTCAACA AGTCTTCAAC TCCCCTTTCC AACAAGGTGT CCCTGGCCGC ATATGTCTTT 
GACACGCTCA CTACCACCAA GACAGCCACA ACGCCGACGG CCTCCAAGGC CGGTCTCTTT 
TTCGAGTTGC AATATGACAC AGCATCCACC ACCAGCCCAC AGCTCATCGG TGCCAAGTTG 
TTGGATCATC GTCTGGAAAG GAGTAGGATA GCGGCTGTGC CAACGGGAGA ACGCAACTTC 
CACGTGCTGT ACTACCTCCT GGCTGGTACA AGTCCCGCGG AAAAGGCCCA TTTGGGATTT 
GAGGAAGGTA CCACAGGGGG TAAGCGGTGG AAGTATCTGG GTCACCCCAC GCAGCTCAAG 
GTTGGCATCA ACGACGCCGA GGGATTTCAG CTTTTCAAGA CGGCCTTGAG GAAGCTCGAG 
TTTCCCAGGA CCGAGATTGC CGAGATCTGC CAGATTTTGG CCGCCATCCT CCATATTGGC 
CAGCTCGAAT TCGAGACAAC ATCGAGCACT TCCGCTACTG GCGATGACAG TGGTGGCTTC 
TCCCATGAGG GTGGCCAGAT GGTTACTGCC GTCAAGAACA AGGATGTTTT GGCCATTGTC 
GCTGCTTTCC TCGGTGTTGG CGTCCAGGAC CTCCAGACAA CGTTGGGTTA CAAGACCAAG 
ATGATCCACA AGGAAAGGGT CACGGTCATG TTGGATCCTG CGGGTGCTCG TGAGAACGCC 
GATGAGTTGG CGCGGACCTT GTACTCGCTA CTCGTCGCTT ACATTATCGA GAACATCAAC 
CAGAAGCTGT GCGCCGCTGA GGATGCCATC TCCAACACTG TTTCCATTAT CGATTTCCCC 
GGCTTCCAGC AGCAGTCTTC TACAGGCTCG ACCCTTGATC AGCTGCTCAA CAATGCTGCT 
GCCGAGTCCA TGTACAACTT GACTCTGCAG ACCTTCTTCG ACCGCAAGGC CGAGCTGCTC 
GAGCTGGAGG AGGTTACTGT CCCCGCGACG AGCTACTTTG ATAATTCGGA TGCCGTGAAG 
GGATTGCTGA AGCCTGGGAA TGGTCTCCTT AGCATCTTGG ATGACCAAAC GCGCCGTCAT 
CGCACTGATA TGCAACTTCT CGAGAGTCTG CGCAAGCGCT TTGAGGGCAA GAACCCCGCT 
ATCGAGGTGG GCTCGGCGAC TGCAAAGTTG CCTGGCAGCA ACTTCTTTAC CGAAAACACC 
GCCGCGACAT TCACGGTCAA GCACTTCGCT GGTGAGGTCG ACTACCCTAT CAAGGGCTTG 
GTGGAGGAGA ATGGTGAGGT TATTTCTGGT GACCTCATGA ACCTCATCAA CTCCAGCAAG 
AGCAACTTTG TCATCCGCCT CTTCGGCCAA GAGGCTCTTC ATACGGTTGT CCACCCGCAA 
GAGAAGACCA CGGTGATGCA AGCCTCAGTC AGCTCTAAGC CGATGCGCAC ACCCAGTGTT 
CTATCGAAGC GAGGACGCCC CGCTCCAGGT CGTCGCCTAC GGGGAGAGAA GGCAGACCCG 
GATATCGCGA TCGACGAGGT CAGCGAGTCG GGAGAGCCCA GCAGGAAGTC GAGCAAGGGA 
TCGGAACAAG GCGCCGCGGC TCAGTTCCTC TCCGCGCTAG AAAACGTCAA GACAGCCTTG 
ACTGCGCAAG GGACAAATTG CTATTTCGTC TTCTGTCTCA AGCCCAACGA CCGGCGTATT 
GCGAACCAGT TTGATAGCAA GTGCGTCAGG GCGCAGGTCC AGACGTTCGG GATTGCCGAG 
ATCAGCCAGC GCCTGCGCGC CGCTGATTTC AGTTTGTTCC TGCCGTTTGG TGAGTTCCTG 
GGCTTGGCAG ATGCCGACAC CATTTTGGTT GGAACTGAAC GCGAAAAGGT CGAGATGGTT 
GTCGAGGACA AGAGATGGCC AAGCAACGAG GTCGCCATCG GCGCTACTGG TGTCTTCTTG 
AGCGAGCGAT GCTGGATGGA ACTGGCACAG CTTGGAGAGA ACACTTCGAC CTCGGGCCGT 
TACGGGGGGT TGCCATCAGA TAATGGTTCT ACACCAGATC CGTTCAGCGA TCCAAAGGAG 
CGCCTGATGC CCTCGACGAG CTCTACACCG CAGATGTACA ACGACAAGAA GGCCGGCTAC 
TTTGGAAGCA ACGATGTGGA TGCACGATCA GAGGCCGGCA CTGCTATCGG CGCGGGCGAC 
ATGTTCAAGA CATTTGAGAC CCGTGAGCAG ATGGCGGAAA AGGCCAATGA AAAGGATATG 
GCCGAGGTTG AGGTGTACAA GGACAAGCCT AGCCGTAAGC GCTGGGTCTT CTTGACCTAT 
TGTTTGACTT GGTTCATTCC CGATTTCGCC ATTCGCATCC TCGGCAAGAT GCCGCGCAAG 
GACGTCCGCA TGGCTTGGAG AGAAAAAGTG GCCATCAACT TCATCATCTG GTTCTTCTGC 
TTGCTTGCCG CTTTCTTCAT TGTTGTTTTT CCCATGTTGA TCTGCCCCAA GCAGCACGTT 
TACAGTCCCG CGGAGCTGTC CAGCTACGAC GGCAAGGGCA AGAACCCTGC CTATGCGGCT 
ATTCGCGGTC AGGTTTTCGA CATTGGTGAC TTCGCGCCAC GCCACTATCC TCGCCTGGAT 
TCAAAGTCCT TCACACAGTA CGCTGGTCTT GACATTTCGG GCCTCTTCCC CGTCCAGGTT 
TCGGCTTTGT GCCGCGGTGT GAAAGGCACG ATTGCGGACA CGGTCACTCT CGACTACAAG 
TCAGACAACA TCACCAACGC GGCCGCTGTT ATCAGCAGCC AGGACAAGAA CGCGCATTTC 
CATGATTTCC GTGCCTTTAC CAATGACAGT CGTCCTGACT GGTTTGCGGA GCAGATGATC 
TTCCTCAAGG GCAATGGCTA CAAGGTTGGC AACGTTGGTT ACTCGCCGCC CTACGTCAAG 
AAGCTCGCTG GCAAGTCACA ATCGATTGCT ATCCTCGAAG GCCGTGTTTA TGACATGAGT 
CAGTACATCA AGGGCGGACG TCACCAACGC GCTGCTCCTG GGAAACCCGA ACCCACGGAT 
CCCAATGCCG TCAACTTCAT GGACCCACTT GTCGTTGATC TTTTCACCCA AAGGGCCGGC 
GAGGATGTCA GTAAGCTCTG GGAGGCTTTG AATCTCGACG CGAACATGAA GGCAGATATG 
AAGCTCTGTC TGGATAACCT CTTTTATGTT GGCGACGTCG ATACTCGTAA CTCGACCCGC 
TGTCAGTTCT CCACGTATCT CGTCTTGGCC ATCTCGATTC TTTTGGCAAC TGTCATCATC 
TTCAAGTTCT TCGCTGCCCT GCAATTCGGC CAGAAGAATA TGCCAGAGAA CCTCGATAAG 
TTCATCATGT GCATGGTTCC GGCTTATACT GAGGACGAAG ACTCACTGCG CCGTGCCATC 
GATTCCGCCG CCCGCATGCA GTACGATGAT AAGCGCAAGC TTCTTATTAT TGTCTGTGAC 
GGTATGATCA TTGGTCAGGG TAACGATAGG CCTACTCCTC GCATTGTACT TGATATTCTG 
GGCGTCGCGG AGACGGTTGA TCCTGAGCCT CTCAGCTTCG AGTCGCTCGG TGAAGGCCAG 
AAGCAGCACA ACATGGGCAA GGTCTACTCG GGTCTTTACG AGGTGCAGGG TCACATCGTT 
CCCTTCATGG TTATCGTCAA GGTTGGCAAG CCTTCTGAGG TCTCTCGTCC CGGTAACCGT 
GGCAAGCGTG ACTCTCAGAT GGTCATGATG CGCTTCCTGA ACCGCGTCCA CTACAACCTC 
CCGATGAGCC CGCTCGAGTT GGAAATGTAC CATCAGATCC GCAACATCAT TGGCGTGAAT 
CCGACCTTCT ACGAGTACAT GCTGCAAATC GATGCCGACA CCGTGGTGGC GCCCGACTCG 
GCGACGCGCT TCGTTGCTGC CTTCCTGGAG GATACCCGTC TTATCGCTGT CTGCGGCGAG 
ACTGCTCTGA CCAACGCCAA GGCCTCCTTC GTTACCATGA TCCAAGTTTA CGAGTACTAC 
ATCTCGCACA ATTTGTCCAA GGCCTTCGAG TCGCTCTTCG GTTCCGTCAC CTGTCTTCCC 
GGCTGTTTCT CCATGTACCG CATTCGCGCT GCCGACTCTG GCAAGCCACT GTTCGTCAGT 
CGCGAGGTCG TTGAGGCTTA TTCCACCATT CGTGTCGACA CTCTGCACAT GAAGAACCTT 
CTTCACCTTG GTGAGGATCG TTACCTGACA ACACTGCTTC TTAAGTTCCA CTCGTCCTAT 
AAGACCAAGT ACATCTTCGC CGCCCACGCC TGGACTATTG CTCCTGACAC GTGGGAAGTT 
TTCCTGTCCC AACGCCGTCG TTGGATCAAC TCTACTGTGC ACAACTTGAT CGAACTCATC 
CCGTTGAACC AGCTTTGCGG TTTCTGCTGC TTCAGCATGA GATTCATCGT GTTTGTCGAC 
TTGCTGTCTA CGGTTGTTCA GCCCGTCACC ATTGCCTACA TTGTCTACTT GATCGTCTTG 
GTGGCGCGTA ATGCGACTGT CGTGCCCATG ACAGCCTTCC TTCTCCTTGG TGCGATTTAC 
GGTTTGCAAG CTATCATCTT CACTCTGCGT CGCAAGTGGG AAATGATTGG TTGGATGGTT 
CTCTACGTTA TCGCCGTTCC CGTCTTCAGT TTCGCCCTTC CTCTCTACTC CTTCTGGCAC 
ATGGACGACT TCAACTGGGG TAACACTCGT GTCGTTGCTG GCGAAGCGGG TCAGAAGATT 
GTCATCTCGG ACGAGGGCAA GTTCGACCCC GCCAGCATCC CCAAGAAGAA GTGGGAGGAG 
TACCAAGCTG AGCTCTGGGA AGCTCAGACC TCTCACGGTG GCAACAACGA CGATATCCGC 
TCCGAAATCT CGGGCTTCTC ATACGCCACC AAGAACCCCT ACAACGTTGC CATGTCCGAG 
TATGGCGGCG GGTACAACTA CGCTAGCCGC CCTGGTTCGA CCACCGGCTT CCCTCTCGGA 
GGTTACAGCG ATAAGACACA TCTGCCCGTT AACAACGGTA GTCGTATGTC TTTCGCCAAC 
TCCGACATGT TAATGGCCGG TAATCGCCAC TCCCAGTTCA GCGGGTCTCA GTTTATGGGC 
CGTACTCCCT CTCAGCAGGA GCTGGAGATG ACCAACATGG CGGGTATGCC GAGCGATGAC 
GCGCTGTTGG CGGAGATTCG CGAGATCTTG AGGACGGCGG ACTTGATGAC GGTTACCAAG 
AAGGGAATCA AGCAGGAACT TGAGAGGAGG TTTGGCGTCC CACTGGACTT GAAGAGGGCT 
TACATTAACA GTGCTACCGA AGCCCTCTTG TCAGGCCAGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_956333.2
CDS	535..6159
Translation	MALNLPPVGGTGGGAHTQPSLPSLPTHLQSDTHLTAHLASRFHVSLPISRLSSHALVSINTYTSSTKGDGTRESSAMGGAEDMAERAYIRLGHRSENQAVLFLGESGSGKTTIRAHVLTALLNKSSTPLSNKVSLAAYVFDTLTTTKTATTPTASKAGLFFELQYDTASTTSPQLIGAKLLDHRLERSRIAAVPTGERNFHVLYYLLAGTSPAEKAHLGFEEGTTGGKRWKYLGHPTQLKVGINDAEGFQLFKTALRKLEFPRTEIAEICQILAAILHIGQLEFETTSSTSATGDDSGGFSHEGGQMVTAVKNKDVLAIVAAFLGVGVQDLQTTLGYKTKMIHKERVTVMLDPAGARENADELARTLYSLLVAYIIENINQKLCAAEDAISNTVSIIDFPGFQQQSSTGSTLDQLLNNAAAESMYNLTLQTFFDRKAELLELEEVTVPATSYFDNSDAVKGLLKPGNGLLSILDDQTRRHRTDMQLLESLRKRFEGKNPAIEVGSATAKLPGSNFFTENTAATFTVKHFAGEVDYPIKGLVEENGEVISGDLMNLINSSKSNFVIRLFGQEALHTVVHPQEKTTVMQASVSSKPMRTPSVLSKRGRPAPGRRLRGEKADPDIAIDEVSESGEPSRKSSKGSEQGAAAQFLSALENVKTALTAQGTNCYFVFCLKPNDRRIANQFDSKCVRAQVQTFGIAEISQRLRAADFSLFLPFGEFLGLADADTILVGTEREKVEMVVEDKRWPSNEVAIGATGVFLSERCWMELAQLGENTSTSGRYGGLPSDNGSTPDPFSDPKERLMPSTSSTPQMYNDKKAGYFGSNDVDARSEAGTAIGAGDMFKTFETREQMAEKANEKDMAEVEVYKDKPSRKRWVFLTYCLTWFIPDFAIRILGKMPRKDVRMAWREKVAINFIIWFFCLLAAFFIVVFPMLICPKQHVYSPAELSSYDGKGKNPAYAAIRGQVFDIGDFAPRHYPRLDSKSFTQYAGLDISGLFPVQVSALCRGVKGTIADTVTLDYKSDNITNAAAVISSQDKNAHFHDFRAFTNDSRPDWFAEQMIFLKGNGYKVGNVGYSPPYVKKLAGKSQSIAILEGRVYDMSQYIKGGRHQRAAPGKPEPTDPNAVNFMDPLVVDLFTQRAGEDVSKLWEALNLDANMKADMKLCLDNLFYVGDVDTRNSTRCQFSTYLVLAISILLATVIIFKFFAALQFGQKNMPENLDKFIMCMVPAYTEDEDSLRRAIDSAARMQYDDKRKLLIIVCDGMIIGQGNDRPTPRIVLDILGVAETVDPEPLSFESLGEGQKQHNMGKVYSGLYEVQGHIVPFMVIVKVGKPSEVSRPGNRGKRDSQMVMMRFLNRVHYNLPMSPLELEMYHQIRNIIGVNPTFYEYMLQIDADTVVAPDSATRFVAAFLEDTRLIAVCGETALTNAKASFVTMIQVYEYYISHNLSKAFESLFGSVTCLPGCFSMYRIRAADSGKPLFVSREVVEAYSTIRVDTLHMKNLLHLGEDRYLTTLLLKFHSSYKTKYIFAAHAWTIAPDTWEVFLSQRRRWINSTVHNLIELIPLNQLCGFCCFSMRFIVFVDLLSTVVQPVTIAYIVYLIVLVARNATVVPMTAFLLLGAIYGLQAIIFTLRRKWEMIGWMVLYVIAVPVFSFALPLYSFWHMDDFNWGNTRVVAGEAGQKIVISDEGKFDPASIPKKKWEEYQAELWEAQTSHGGNNDDIRSEISGFSYATKNPYNVAMSEYGGGYNYASRPGSTTGFPLGGYSDKTHLPVNNGSRMSFANSDMLMAGNRHSQFSGSQFMGRTPSQQELEMTNMAGMPSDDALLAEIREILRTADLMTVTKKGIKQELERRFGVPLDLKRAYINSATEALLSGQL

Target ORF information:

RefSeq Version	XM_951240.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A chitin synthase C (chs-5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_951240.3

ORF Insert Sequence:

ATGGCGCTCA ACCTTCCTCC CGTAGGGGGG ACGGGTGGAG GGGCCCATAC CCAACCTTCG 
TTACCTTCGC TGCCAACCCA TCTTCAGTCG GATACACACT TGACGGCTCA CCTGGCCAGT 
CGATTCCACG TCTCGCTACC GATTTCCCGA CTTTCATCCC ACGCACTCGT TTCCATCAAC 
ACATACACTA GCTCCACGAA AGGAGATGGC ACGCGAGAGT CTTCCGCCAT GGGCGGTGCC 
GAGGACATGG CCGAGAGGGC CTACATCCGT CTCGGTCATC GGTCAGAGAA CCAGGCCGTG 
CTCTTCTTGG GTGAATCGGG CTCGGGAAAG ACGACCATTC GTGCGCATGT CCTGACCGCA 
CTCCTCAACA AGTCTTCAAC TCCCCTTTCC AACAAGGTGT CCCTGGCCGC ATATGTCTTT 
GACACGCTCA CTACCACCAA GACAGCCACA ACGCCGACGG CCTCCAAGGC CGGTCTCTTT 
TTCGAGTTGC AATATGACAC AGCATCCACC ACCAGCCCAC AGCTCATCGG TGCCAAGTTG 
TTGGATCATC GTCTGGAAAG GAGTAGGATA GCGGCTGTGC CAACGGGAGA ACGCAACTTC 
CACGTGCTGT ACTACCTCCT GGCTGGTACA AGTCCCGCGG AAAAGGCCCA TTTGGGATTT 
GAGGAAGGTA CCACAGGGGG TAAGCGGTGG AAGTATCTGG GTCACCCCAC GCAGCTCAAG 
GTTGGCATCA ACGACGCCGA GGGATTTCAG CTTTTCAAGA CGGCCTTGAG GAAGCTCGAG 
TTTCCCAGGA CCGAGATTGC CGAGATCTGC CAGATTTTGG CCGCCATCCT CCATATTGGC 
CAGCTCGAAT TCGAGACAAC ATCGAGCACT TCCGCTACTG GCGATGACAG TGGTGGCTTC 
TCCCATGAGG GTGGCCAGAT GGTTACTGCC GTCAAGAACA AGGATGTTTT GGCCATTGTC 
GCTGCTTTCC TCGGTGTTGG CGTCCAGGAC CTCCAGACAA CGTTGGGTTA CAAGACCAAG 
ATGATCCACA AGGAAAGGGT CACGGTCATG TTGGATCCTG CGGGTGCTCG TGAGAACGCC 
GATGAGTTGG CGCGGACCTT GTACTCGCTA CTCGTCGCTT ACATTATCGA GAACATCAAC 
CAGAAGCTGT GCGCCGCTGA GGATGCCATC TCCAACACTG TTTCCATTAT CGATTTCCCC 
GGCTTCCAGC AGCAGTCTTC TACAGGCTCG ACCCTTGATC AGCTGCTCAA CAATGCTGCT 
GCCGAGTCCA TGTACAACTT GACTCTGCAG ACCTTCTTCG ACCGCAAGGC CGAGCTGCTC 
GAGCTGGAGG AGGTTACTGT CCCCGCGACG AGCTACTTTG ATAATTCGGA TGCCGTGAAG 
GGATTGCTGA AGCCTGGGAA TGGTCTCCTT AGCATCTTGG ATGACCAAAC GCGCCGTCAT 
CGCACTGATA TGCAACTTCT CGAGAGTCTG CGCAAGCGCT TTGAGGGCAA GAACCCCGCT 
ATCGAGGTGG GCTCGGCGAC TGCAAAGTTG CCTGGCAGCA ACTTCTTTAC CGAAAACACC 
GCCGCGACAT TCACGGTCAA GCACTTCGCT GGTGAGGTCG ACTACCCTAT CAAGGGCTTG 
GTGGAGGAGA ATGGTGAGGT TATTTCTGGT GACCTCATGA ACCTCATCAA CTCCAGCAAG 
AGCAACTTTG TCATCCGCCT CTTCGGCCAA GAGGCTCTTC ATACGGTTGT CCACCCGCAA 
GAGAAGACCA CGGTGATGCA AGCCTCAGTC AGCTCTAAGC CGATGCGCAC ACCCAGTGTT 
CTATCGAAGC GAGGACGCCC CGCTCCAGGT CGTCGCCTAC GGGGAGAGAA GGCAGACCCG 
GATATCGCGA TCGACGAGGT CAGCGAGTCG GGAGAGCCCA GCAGGAAGTC GAGCAAGGGA 
TCGGAACAAG GCGCCGCGGC TCAGTTCCTC TCCGCGCTAG AAAACGTCAA GACAGCCTTG 
ACTGCGCAAG GGACAAATTG CTATTTCGTC TTCTGTCTCA AGCCCAACGA CCGGCGTATT 
GCGAACCAGT TTGATAGCAA GTGCGTCAGG GCGCAGGTCC AGACGTTCGG GATTGCCGAG 
ATCAGCCAGC GCCTGCGCGC CGCTGATTTC AGTTTGTTCC TGCCGTTTGG TGAGTTCCTG 
GGCTTGGCAG ATGCCGACAC CATTTTGGTT GGAACTGAAC GCGAAAAGGT CGAGATGGTT 
GTCGAGGACA AGAGATGGCC AAGCAACGAG GTCGCCATCG GCGCTACTGG TGTCTTCTTG 
AGCGAGCGAT GCTGGATGGA ACTGGCACAG CTTGGAGAGA ACACTTCGAC CTCGGGCCGT 
TACGGGGGGT TGCCATCAGA TAATGGTTCT ACACCAGATC CGTTCAGCGA TCCAAAGGAG 
CGCCTGATGC CCTCGACGAG CTCTACACCG CAGATGTACA ACGACAAGAA GGCCGGCTAC 
TTTGGAAGCA ACGATGTGGA TGCACGATCA GAGGCCGGCA CTGCTATCGG CGCGGGCGAC 
ATGTTCAAGA CATTTGAGAC CCGTGAGCAG ATGGCGGAAA AGGCCAATGA AAAGGATATG 
GCCGAGGTTG AGGTGTACAA GGACAAGCCT AGCCGTAAGC GCTGGGTCTT CTTGACCTAT 
TGTTTGACTT GGTTCATTCC CGATTTCGCC ATTCGCATCC TCGGCAAGAT GCCGCGCAAG 
GACGTCCGCA TGGCTTGGAG AGAAAAAGTG GCCATCAACT TCATCATCTG GTTCTTCTGC 
TTGCTTGCCG CTTTCTTCAT TGTTGTTTTT CCCATGTTGA TCTGCCCCAA GCAGCACGTT 
TACAGTCCCG CGGAGCTGTC CAGCTACGAC GGCAAGGGCA AGAACCCTGC CTATGCGGCT 
ATTCGCGGTC AGGTTTTCGA CATTGGTGAC TTCGCGCCAC GCCACTATCC TCGCCTGGAT 
TCAAAGTCCT TCACACAGTA CGCTGGTCTT GACATTTCGG GCCTCTTCCC CGTCCAGGTT 
TCGGCTTTGT GCCGCGGTGT GAAAGGCACG ATTGCGGACA CGGTCACTCT CGACTACAAG 
TCAGACAACA TCACCAACGC GGCCGCTGTT ATCAGCAGCC AGGACAAGAA CGCGCATTTC 
CATGATTTCC GTGCCTTTAC CAATGACAGT CGTCCTGACT GGTTTGCGGA GCAGATGATC 
TTCCTCAAGG GCAATGGCTA CAAGGTTGGC AACGTTGGTT ACTCGCCGCC CTACGTCAAG 
AAGCTCGCTG GCAAGTCACA ATCGATTGCT ATCCTCGAAG GCCGTGTTTA TGACATGAGT 
CAGTACATCA AGGGCGGACG TCACCAACGC GCTGCTCCTG GGAAACCCGA ACCCACGGAT 
CCCAATGCCG TCAACTTCAT GGACCCACTT GTCGTTGATC TTTTCACCCA AAGGGCCGGC 
GAGGATGTCA GTAAGCTCTG GGAGGCTTTG AATCTCGACG CGAACATGAA GGCAGATATG 
AAGCTCTGTC TGGATAACCT CTTTTATGTT GGCGACGTCG ATACTCGTAA CTCGACCCGC 
TGTCAGTTCT CCACGTATCT CGTCTTGGCC ATCTCGATTC TTTTGGCAAC TGTCATCATC 
TTCAAGTTCT TCGCTGCCCT GCAATTCGGC CAGAAGAATA TGCCAGAGAA CCTCGATAAG 
TTCATCATGT GCATGGTTCC GGCTTATACT GAGGACGAAG ACTCACTGCG CCGTGCCATC 
GATTCCGCCG CCCGCATGCA GTACGATGAT AAGCGCAAGC TTCTTATTAT TGTCTGTGAC 
GGTATGATCA TTGGTCAGGG TAACGATAGG CCTACTCCTC GCATTGTACT TGATATTCTG 
GGCGTCGCGG AGACGGTTGA TCCTGAGCCT CTCAGCTTCG AGTCGCTCGG TGAAGGCCAG 
AAGCAGCACA ACATGGGCAA GGTCTACTCG GGTCTTTACG AGGTGCAGGG TCACATCGTT 
CCCTTCATGG TTATCGTCAA GGTTGGCAAG CCTTCTGAGG TCTCTCGTCC CGGTAACCGT 
GGCAAGCGTG ACTCTCAGAT GGTCATGATG CGCTTCCTGA ACCGCGTCCA CTACAACCTC 
CCGATGAGCC CGCTCGAGTT GGAAATGTAC CATCAGATCC GCAACATCAT TGGCGTGAAT 
CCGACCTTCT ACGAGTACAT GCTGCAAATC GATGCCGACA CCGTGGTGGC GCCCGACTCG 
GCGACGCGCT TCGTTGCTGC CTTCCTGGAG GATACCCGTC TTATCGCTGT CTGCGGCGAG 
ACTGCTCTGA CCAACGCCAA GGCCTCCTTC GTTACCATGA TCCAAGTTTA CGAGTACTAC 
ATCTCGCACA ATTTGTCCAA GGCCTTCGAG TCGCTCTTCG GTTCCGTCAC CTGTCTTCCC 
GGCTGTTTCT CCATGTACCG CATTCGCGCT GCCGACTCTG GCAAGCCACT GTTCGTCAGT 
CGCGAGGTCG TTGAGGCTTA TTCCACCATT CGTGTCGACA CTCTGCACAT GAAGAACCTT 
CTTCACCTTG GTGAGGATCG TTACCTGACA ACACTGCTTC TTAAGTTCCA CTCGTCCTAT 
AAGACCAAGT ACATCTTCGC CGCCCACGCC TGGACTATTG CTCCTGACAC GTGGGAAGTT 
TTCCTGTCCC AACGCCGTCG TTGGATCAAC TCTACTGTGC ACAACTTGAT CGAACTCATC 
CCGTTGAACC AGCTTTGCGG TTTCTGCTGC TTCAGCATGA GATTCATCGT GTTTGTCGAC 
TTGCTGTCTA CGGTTGTTCA GCCCGTCACC ATTGCCTACA TTGTCTACTT GATCGTCTTG 
GTGGCGCGTA ATGCGACTGT CGTGCCCATG ACAGCCTTCC TTCTCCTTGG TGCGATTTAC 
GGTTTGCAAG CTATCATCTT CACTCTGCGT CGCAAGTGGG AAATGATTGG TTGGATGGTT 
CTCTACGTTA TCGCCGTTCC CGTCTTCAGT TTCGCCCTTC CTCTCTACTC CTTCTGGCAC 
ATGGACGACT TCAACTGGGG TAACACTCGT GTCGTTGCTG GCGAAGCGGG TCAGAAGATT 
GTCATCTCGG ACGAGGGCAA GTTCGACCCC GCCAGCATCC CCAAGAAGAA GTGGGAGGAG 
TACCAAGCTG AGCTCTGGGA AGCTCAGACC TCTCACGGTG GCAACAACGA CGATATCCGC 
TCCGAAATCT CGGGCTTCTC ATACGCCACC AAGAACCCCT ACAACGTTGC CATGTCCGAG 
TATGGCGGCG GGTACAACTA CGCTAGCCGC CCTGGTTCGA CCACCGGCTT CCCTCTCGGA 
GGTTACAGCG ATAAGACACA TCTGCCCGTT AACAACGGTA GTCGTATGTC TTTCGCCAAC 
TCCGACATGT TAATGGCCGG TAATCGCCAC TCCCAGTTCA GCGGGTCTCA GTTTATGGGC 
CGTACTCCCT CTCAGCAGGA GCTGGAGATG ACCAACATGG CGGGTATGCC GAGCGATGAC 
GCGCTGTTGG CGGAGATTCG CGAGATCTTG AGGACGGCGG ACTTGATGAC GGTTACCAAG 
AAGGGAATCA AGCAGGAACT TGAGAGGAGG TTTGGCGTCC CACTGGACTT GAAGAGGGCT 
TACATTAACA GTGCTACCGA AGCCCTCTTG TCAGGCCAGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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chs-5 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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