MGG_06395 cDNA ORF clone, Pyricularia oryzae 70-15

The following MGG_06395 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MGG_06395 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMe07933 XM_003717128.1
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Pyricularia oryzae 70-15 intron-binding protein aquarius (MGG_06395), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMe07933
    Clone ID Related Accession (Same CDS sequence) XM_003717128.1
    Accession Version XM_003717128.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4335bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-28
    Organism Pyricularia oryzae 70-15
    Product intron-binding protein aquarius
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_017851).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCCAACCG CGAAGAGACA AAAGGGTGCG CGAAAGTCAG AAAACAACGA AGGTAGCTCG 
    ACTCACCCCC CATCGACTGC CACGAATCAA CGCGGCGGAG AGTCCGAATT CTCTCAGCTT
    GCCCATCAAC ACTGGCTCAA ACCATCCAAG CGGACCACCA AGGTCAAGAT CAAGAATGAT
    GTTCTCAAGA GTATGTGGGA CTCGTTGGAG CGGGATCAGT TCCCGCACAA GTCCTTATTA
    GAGCTGGAAA CTCTTCAGCT CCTCGAAAGT TATCTTTGGC CAGGATACAC AGAGAACTCT
    TCACACCACC ATGTCTTGCT CACGGTTCTT ATTGTGAATA CAAAGCGAAG GGAGCGACTC
    GATACCTGGG CTCTGTTCGA GGACAGACCA GTGGACTTCT CAAGTCTTTT CCGCCGTGTA
    CTGTCCATGA GTATCGACCA GACGCTGAGT ATAGCTACCA GAACTCAGAT TATGTGTTTT
    GTAATCCAGG GATTTCAATC TCTGGATTGT GGAATTGTCC GGAAAGAATG TGCTCCCCTT
    GTGTCAATAT CAATATGGCA CAATCTGGTG ACCGAGGAGA AGCGGGACCA TCTCCTAGAT
    CAGGATTTGC ACTTGCACAA GGCGTGGAGA GCTGCTTTAA GAAGGTACGA GGCCGCAGAC
    GATGCTACAA AGGCCAGACT TCGTTTCGAG AGGTCTTGGC TCTACACGCT TGTTTTGGAC
    TTCTATTCGC TCTTATATGG TGGCAACTCT ACCCCAGATG TCGTAATCTA CTGCGAGCGC
    TTCATGGAGC TTCTTATAGA TCTTCAGAGC CAACTACCAA CAAGGCGCTA TGTCAACACC
    CTGCTACAGG ATCTTCATAT TTCTACCGCC ATAACTATAT CTCCACTGTA CAACGACGAA
    GACCATGGTC TTTTCCGCGA TCTCCATGGA CTGTTTCAAC ACTTCACCTT CTTCATAATA
    AACGATTATA CGGGGGCGCA GCTGAGCTCA GCTGAAGCCT ATGGCCGCAA GTGTGCGGAG
    CTTGCAAAGC TACAGCGCAT AGCATTCCAG CATTTCAGAG AGAAGCTGTT GGTTCTAGCG
    CTCTCAAACT ATGGCTCTAT CGGAGACAGG GGAGAACTTG TAACCCTCTT GGAACCGTTG
    ACAGACGACG AGGTGTCTAG GCTGGCTTCT TTGCTGCTTC TGCGTACATC ATACCCGGAT
    GCAGCCAAAC TGCCGGTTGA CCGCAAATTC CTAATTGAGG TCCTACTCTC TGCATTCGAG
    AAGCGGCAAA CCTTCCGGGA TGTGATTCGT GACATGAACA TCATGCCCAC GGAACAGACA
    CTGTTCGAAA GTAGCCTATT ACGCACCGAC AGTTACAATG GATCTCAACC GCTTGCTTTG
    CCAAAACTCA ATCTCCAATA TCTCTCGATC GGGGACTTCC TGTGGCGGGC GTTTGTTCTT
    TACAGGTGTG AGTCCTTCTA TGGTATTCGC AAGGATGTCG AGGCCGCCCT GCAAAGGTTG
    AAGCCGATCT CACCAAGACC TGGAGAAACT TCTTTCACAG GAAACTCCCG GATGGCTTTA
    CCAGTCAGCA AGCCAGCGGT TGTTGATGTG GTACCAGCTC AAGTTGGCGA CGACGCACCT
    TCATTAGTCA AGGCTGAGGT CACCATTGAC CTACGTCGCC TTACTGACGG TGTGAAAAGG
    GATTGGGACT CGTTAAGGCC AGATGATGTC GTATTCCTTT TGGCAGTCGA TGCATCAACT
    TCACAGGAGA ATGAGACAGG CGGACCACCT CTTGGTGAAG CTCAAAAGCA GGGATTGCTG
    GCAGTTCGTA CCGCCGAGGT CATGCAAATT GTCGATGACC GAGGTCGCGC CGTCAGAGAT
    GATGGCTTTG GGCGAGGAGC AATCCGCAGA CTGATCCTCA AACTCGACCC GAATATGTAC
    AAGGCTGACA TTGAAGGTCC GTCATCCGCC AAGTCAGACA TTTATCAACG ATTCAATGTC
    GTAGTTCGAC GAAGCGGGAG AGAAAACAAC TTCAAACCTG TCTTGGAATC TATCAGAAGC
    CTGGCTCTCA CCGATGTGCC CTTGGCTCCA TGGCTGCACG AGGTTTTCCT AGGCTACGGG
    GACCCTGCCG CGGCTACCTT CAAGCTTCTC CCCAACCGGA TCAAGACTCT GGATTACAGG
    GATACATTCC TCGATTGGCA GCATCTTGTT GCTAGCCTGC CTGGCAGGAT AGTCGAGCCT
    GGTTTTGACG TGGATGGTAG CTTTGGTCCT CCTTACGTCC TGACGACAGC GGACAAGCCC
    GAAGAAGAGG TTTCGACCAA ACAGTCAAAG AAGCGACGGA GAGATGCAGA GCCTGCCCTC
    ATATCGGAAG TAGAGACGCT CAAAGTCTCT ACCTACAAGA CGCCAAACAA CGGACCGTAC
    CCTACTGATA GACTGAAGAG GAACAACGTT CGTTTCACCC CTGCCCAGAT TGACGCCATC
    ATATCAGGCA GTCAGCCCGG CTTGACTGTC ATCGTTGGGC CACCTGGAAC TGGCAAGACC
    GATGTGGCTA CCCAGATCAT CAACAACATC TACCACAACT TTCCCGAGCA GCGGACACTG
    CTTATCGCCC ATAGCAACCA GGCACTAAAC CAGCTGTTCA CCAAGATTGT TGCTCTTGAC
    ATTGACGAGC GCCACTTGTT GCGCTTGGGA CATGGCGAAG AAGACCTTGA CGCTGGGGGC
    AACTTTGGCA AGGCTGGACG GGTGGAATCT TTCCTCAAAA ACCGGGACAG GTATCTGTTA
    GAGGTTACCA AGCTGGCGGT CAGTATTGGT GCTCCTGGGG CCCATGGTAA CTCAGCAGAG
    ACCGCGGGCC ACTTTAACTC TGTGTATGTT GAGCCGGCCT GGGCCAGGTT CTTGGCGTTC
    ACCGACGACC CCTCGCTTTC TGCTGCGGAG GTTGCGGCGG CATTTCCGTT CACCGATTTC
    TTCGCCGATG CACCCCAGCC TCTATTTCCC CCGGAAGGAG ACCGAGAAGC GGTTTCAGAC
    ATTATTAACG GATGTTACCG ACACATCTCA AGGATATTTG CAGAGCTGGC TGACATCCTA
    CCTTTTGAGA TACTTCGACG TGAGAAGGAT AAGGCAAATT ACCTCCTGAC TAGCGAGGCG
    CGCATCGTTG CAATGACATC TACCCACGCC GCAATAAGGC GAGGAGAGAT TGCCTCTCTT
    GGATTCAAGT ACGACAATGT CGTGGTGGAG GAGGCCGCAC AAATCACTGA AATCGAGAGT
    TTCATTCCTC TAGCCATGCA AAAGCCCAAA GCTGGCCACA ATGGACTTCA ACGCATAGTG
    CTGTGTGGTG ATCACTTGCA GAACTCGCCC GTGATTCAGA ATATGGCGTT CCGTCACTAC
    GCGAACCTGG AGCAATCGCT TTTCTCACGT CTTGTACGCC TCGGGGTTCC GACAATAAGC
    TTGGATCAGC AAGGACGTGC TCGGCCGTCG ATTGCCAAGC TGTACCAGTG GAGGTACAAC
    AATCTGGGGA ACCTGCCTCA TGTTGAGACC AGCGACGAAT TCTTGACCGC GAACGCTGGG
    TTCAAGTACG ATTACCAATT CATCAACGTT CCGGACTACA AAGGCAAGGG GGAGTCTGAG
    CCGTCACCTC ATTTCATCCA GAACTTAGGC GAGGCCGAGT ACGCAGTTGC AATCTACCAG
    TACATGAGGC TCCTTGGGTA CCCGGCGAAT AAAATCAGCA TTCTTACAAC ATATGCTGGT
    CAGCGTGCCC TGGTACGTGA TGTGTTGAAA CATCGTTGTG GGCGAGGTCA AATCTTTGGC
    CTGCCAAAGA TTGTCACCAC AGTTGATAAG TACCAGGGCG AGCAGAATGA CTATATTATT
    CTGTCACTGA CCCGCACAGC GCGGGTGGGT TACCTCAGAG ATGTCCGCCG TTTGACTGTC
    GCCCTCTCAC GAGCTCGACT TGGTCTTTAC GTTGTTGGGC GGCGACAAGT CTTTGAGTCT
    TGCCCAGAGC TGAGGCAGGC ATTTGATATC CTTCTGCAGC GTCCCGATAA GTTGAGCCTG
    GTTACAGGAG AGCTGTGGCC CTCGCAAAGG AGCCTTGCGG ATGAAACCGA CAAAGAAACC
    GTACCAGGAG AAGTAGTCAT GGAAGACGTG GTGCACATGG GTCAGTATGT GTACGAAATG
    ACAGTGACTA AAATGGGGCA GATTGGTACA GGAAAGGGGC TGCCGATGTT GGAAGAGGTG
    GAGGAGGAGG CTGCAGGTGC AGTGGAAGCT CTCGCGCCCC CTCCAGCAGC CTCGGAGCTA
    ATGGAGGTCA CAGAAGACAG GGAAGTGGAA GGGATAGATC CGGAGATAGA AGATGCAGGG
    GTGGCAGCAA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003717176.1
    CDS129..4463
    Translation

    Target ORF information:

    RefSeq Version XM_003717128.1
    Organism Pyricularia oryzae 70-15
    Definition Pyricularia oryzae 70-15 intron-binding protein aquarius (MGG_06395), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003717128.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCCAACCG CGAAGAGACA AAAGGGTGCG CGAAAGTCAG AAAACAACGA AGGTAGCTCG 
    ACTCACCCCC CATCGACTGC CACGAATCAA CGCGGCGGAG AGTCCGAATT CTCTCAGCTT
    GCCCATCAAC ACTGGCTCAA ACCATCCAAG CGGACCACCA AGGTCAAGAT CAAGAATGAT
    GTTCTCAAGA GTATGTGGGA CTCGTTGGAG CGGGATCAGT TCCCGCACAA GTCCTTATTA
    GAGCTGGAAA CTCTTCAGCT CCTCGAAAGT TATCTTTGGC CAGGATACAC AGAGAACTCT
    TCACACCACC ATGTCTTGCT CACGGTTCTT ATTGTGAATA CAAAGCGAAG GGAGCGACTC
    GATACCTGGG CTCTGTTCGA GGACAGACCA GTGGACTTCT CAAGTCTTTT CCGCCGTGTA
    CTGTCCATGA GTATCGACCA GACGCTGAGT ATAGCTACCA GAACTCAGAT TATGTGTTTT
    GTAATCCAGG GATTTCAATC TCTGGATTGT GGAATTGTCC GGAAAGAATG TGCTCCCCTT
    GTGTCAATAT CAATATGGCA CAATCTGGTG ACCGAGGAGA AGCGGGACCA TCTCCTAGAT
    CAGGATTTGC ACTTGCACAA GGCGTGGAGA GCTGCTTTAA GAAGGTACGA GGCCGCAGAC
    GATGCTACAA AGGCCAGACT TCGTTTCGAG AGGTCTTGGC TCTACACGCT TGTTTTGGAC
    TTCTATTCGC TCTTATATGG TGGCAACTCT ACCCCAGATG TCGTAATCTA CTGCGAGCGC
    TTCATGGAGC TTCTTATAGA TCTTCAGAGC CAACTACCAA CAAGGCGCTA TGTCAACACC
    CTGCTACAGG ATCTTCATAT TTCTACCGCC ATAACTATAT CTCCACTGTA CAACGACGAA
    GACCATGGTC TTTTCCGCGA TCTCCATGGA CTGTTTCAAC ACTTCACCTT CTTCATAATA
    AACGATTATA CGGGGGCGCA GCTGAGCTCA GCTGAAGCCT ATGGCCGCAA GTGTGCGGAG
    CTTGCAAAGC TACAGCGCAT AGCATTCCAG CATTTCAGAG AGAAGCTGTT GGTTCTAGCG
    CTCTCAAACT ATGGCTCTAT CGGAGACAGG GGAGAACTTG TAACCCTCTT GGAACCGTTG
    ACAGACGACG AGGTGTCTAG GCTGGCTTCT TTGCTGCTTC TGCGTACATC ATACCCGGAT
    GCAGCCAAAC TGCCGGTTGA CCGCAAATTC CTAATTGAGG TCCTACTCTC TGCATTCGAG
    AAGCGGCAAA CCTTCCGGGA TGTGATTCGT GACATGAACA TCATGCCCAC GGAACAGACA
    CTGTTCGAAA GTAGCCTATT ACGCACCGAC AGTTACAATG GATCTCAACC GCTTGCTTTG
    CCAAAACTCA ATCTCCAATA TCTCTCGATC GGGGACTTCC TGTGGCGGGC GTTTGTTCTT
    TACAGGTGTG AGTCCTTCTA TGGTATTCGC AAGGATGTCG AGGCCGCCCT GCAAAGGTTG
    AAGCCGATCT CACCAAGACC TGGAGAAACT TCTTTCACAG GAAACTCCCG GATGGCTTTA
    CCAGTCAGCA AGCCAGCGGT TGTTGATGTG GTACCAGCTC AAGTTGGCGA CGACGCACCT
    TCATTAGTCA AGGCTGAGGT CACCATTGAC CTACGTCGCC TTACTGACGG TGTGAAAAGG
    GATTGGGACT CGTTAAGGCC AGATGATGTC GTATTCCTTT TGGCAGTCGA TGCATCAACT
    TCACAGGAGA ATGAGACAGG CGGACCACCT CTTGGTGAAG CTCAAAAGCA GGGATTGCTG
    GCAGTTCGTA CCGCCGAGGT CATGCAAATT GTCGATGACC GAGGTCGCGC CGTCAGAGAT
    GATGGCTTTG GGCGAGGAGC AATCCGCAGA CTGATCCTCA AACTCGACCC GAATATGTAC
    AAGGCTGACA TTGAAGGTCC GTCATCCGCC AAGTCAGACA TTTATCAACG ATTCAATGTC
    GTAGTTCGAC GAAGCGGGAG AGAAAACAAC TTCAAACCTG TCTTGGAATC TATCAGAAGC
    CTGGCTCTCA CCGATGTGCC CTTGGCTCCA TGGCTGCACG AGGTTTTCCT AGGCTACGGG
    GACCCTGCCG CGGCTACCTT CAAGCTTCTC CCCAACCGGA TCAAGACTCT GGATTACAGG
    GATACATTCC TCGATTGGCA GCATCTTGTT GCTAGCCTGC CTGGCAGGAT AGTCGAGCCT
    GGTTTTGACG TGGATGGTAG CTTTGGTCCT CCTTACGTCC TGACGACAGC GGACAAGCCC
    GAAGAAGAGG TTTCGACCAA ACAGTCAAAG AAGCGACGGA GAGATGCAGA GCCTGCCCTC
    ATATCGGAAG TAGAGACGCT CAAAGTCTCT ACCTACAAGA CGCCAAACAA CGGACCGTAC
    CCTACTGATA GACTGAAGAG GAACAACGTT CGTTTCACCC CTGCCCAGAT TGACGCCATC
    ATATCAGGCA GTCAGCCCGG CTTGACTGTC ATCGTTGGGC CACCTGGAAC TGGCAAGACC
    GATGTGGCTA CCCAGATCAT CAACAACATC TACCACAACT TTCCCGAGCA GCGGACACTG
    CTTATCGCCC ATAGCAACCA GGCACTAAAC CAGCTGTTCA CCAAGATTGT TGCTCTTGAC
    ATTGACGAGC GCCACTTGTT GCGCTTGGGA CATGGCGAAG AAGACCTTGA CGCTGGGGGC
    AACTTTGGCA AGGCTGGACG GGTGGAATCT TTCCTCAAAA ACCGGGACAG GTATCTGTTA
    GAGGTTACCA AGCTGGCGGT CAGTATTGGT GCTCCTGGGG CCCATGGTAA CTCAGCAGAG
    ACCGCGGGCC ACTTTAACTC TGTGTATGTT GAGCCGGCCT GGGCCAGGTT CTTGGCGTTC
    ACCGACGACC CCTCGCTTTC TGCTGCGGAG GTTGCGGCGG CATTTCCGTT CACCGATTTC
    TTCGCCGATG CACCCCAGCC TCTATTTCCC CCGGAAGGAG ACCGAGAAGC GGTTTCAGAC
    ATTATTAACG GATGTTACCG ACACATCTCA AGGATATTTG CAGAGCTGGC TGACATCCTA
    CCTTTTGAGA TACTTCGACG TGAGAAGGAT AAGGCAAATT ACCTCCTGAC TAGCGAGGCG
    CGCATCGTTG CAATGACATC TACCCACGCC GCAATAAGGC GAGGAGAGAT TGCCTCTCTT
    GGATTCAAGT ACGACAATGT CGTGGTGGAG GAGGCCGCAC AAATCACTGA AATCGAGAGT
    TTCATTCCTC TAGCCATGCA AAAGCCCAAA GCTGGCCACA ATGGACTTCA ACGCATAGTG
    CTGTGTGGTG ATCACTTGCA GAACTCGCCC GTGATTCAGA ATATGGCGTT CCGTCACTAC
    GCGAACCTGG AGCAATCGCT TTTCTCACGT CTTGTACGCC TCGGGGTTCC GACAATAAGC
    TTGGATCAGC AAGGACGTGC TCGGCCGTCG ATTGCCAAGC TGTACCAGTG GAGGTACAAC
    AATCTGGGGA ACCTGCCTCA TGTTGAGACC AGCGACGAAT TCTTGACCGC GAACGCTGGG
    TTCAAGTACG ATTACCAATT CATCAACGTT CCGGACTACA AAGGCAAGGG GGAGTCTGAG
    CCGTCACCTC ATTTCATCCA GAACTTAGGC GAGGCCGAGT ACGCAGTTGC AATCTACCAG
    TACATGAGGC TCCTTGGGTA CCCGGCGAAT AAAATCAGCA TTCTTACAAC ATATGCTGGT
    CAGCGTGCCC TGGTACGTGA TGTGTTGAAA CATCGTTGTG GGCGAGGTCA AATCTTTGGC
    CTGCCAAAGA TTGTCACCAC AGTTGATAAG TACCAGGGCG AGCAGAATGA CTATATTATT
    CTGTCACTGA CCCGCACAGC GCGGGTGGGT TACCTCAGAG ATGTCCGCCG TTTGACTGTC
    GCCCTCTCAC GAGCTCGACT TGGTCTTTAC GTTGTTGGGC GGCGACAAGT CTTTGAGTCT
    TGCCCAGAGC TGAGGCAGGC ATTTGATATC CTTCTGCAGC GTCCCGATAA GTTGAGCCTG
    GTTACAGGAG AGCTGTGGCC CTCGCAAAGG AGCCTTGCGG ATGAAACCGA CAAAGAAACC
    GTACCAGGAG AAGTAGTCAT GGAAGACGTG GTGCACATGG GTCAGTATGT GTACGAAATG
    ACAGTGACTA AAATGGGGCA GATTGGTACA GGAAAGGGGC TGCCGATGTT GGAAGAGGTG
    GAGGAGGAGG CTGCAGGTGC AGTGGAAGCT CTCGCGCCCC CTCCAGCAGC CTCGGAGCTA
    ATGGAGGTCA CAGAAGACAG GGAAGTGGAA GGGATAGATC CGGAGATAGA AGATGCAGGG
    GTGGCAGCAA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the rice blast fungus Magnaporthe grisea.
    Nature434(7036)980-6(2005 Apr)
    Dean RA,Talbot NJ,Ebbole DJ,Farman ML,Mitchell TK,Orbach MJ,Thon M,Kulkarni R,Xu JR,Pan H,Read ND,Lee YH,Carbone I,Brown D,Oh YY,Donofrio N,Jeong JS,Soanes DM,Djonovic S,Kolomiets E,Rehmeyer C,Li W,Harding M,Kim S,Lebrun MH,Bohnert H,Coughlan S,Butler J,Calvo S,Ma LJ,Nicol R,Purcell S,Nusbaum C,Galagan JE,Birren BW