コントロール抗体

製品の詳細

アイソタイプコントロール抗体

ローディングコントロール抗体

事例

• 1. 感度比較
  

  Primary Antibody:

  • 0.1 μg/ml THE™ β-Actin mAb(Mouse)(GenScript, A00702)
  • 0.1 μg/ml β-Actin mAb (Mouse) (Company X)

  Secondary Antibody:

  • Goat Anti-Mouse IgG (H&L) [HRP] pAb (GenScript, 1:10,000, A00160)
  • The signal was developed with Lumisensor HRP Substrate Kit (GenScript, L00221V500)
• 2. 免疫蛍光染色解析
  

  Primary Antibody:

  • 0.1 μg/ml THE™ β-Tubulin mAb (GenScript, A01410)

  Secondary Antibody:

  • Fluorescein conjugated affinity purified anti-Mouse IgG (H&L) (Goat) (Rockland, 1:1,000, 610-102-121)
• 3. 蛍光ウェスタンブロッティング
  

  Primary Antibody:

  • 0.1 μg/ml GAPDH Antibody, pAb, Goat (GenScript, A00191)

  Lane 1-8:

  • 5.0, 2.5,1.25, 0.62, 0.31, 0.16, 0.08 and 0.04 μg of Hela cell lysate
  • The signal was developed with One-Step Western Fluorescent Kit (GenScript, L00397)