THE™ beta Actin Antibody, mAb, Mouse

THE™ beta Actin Antibody, mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig.It has not yet been tested in other species.

Cat. No.	A00702
Size	100 μg : Quote 200 μg : Quote Buy in bulk
Quantity
Price	¥34,524.00


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Overview

Specificity	THE™ beta Actin Antibody, mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig.It has not yet been tested in other species.
Host Species	Mouse
Immunogen	A synthetic peptide DDDIAALVVDNGSG coupled to KLH
Species Reactivity	Mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig
Conjugate	Unconjugated

Applications

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.1-1.0 µg/ml
Western Blot	0.1-1.0 µg/ml

Properties

Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.Note: 100 µg, 200µg (GenScript, A00702), lyophilized with PBS, pH 7.4, containing 0.02% sodium azide, without BSA
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions	The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A or G affinity column
Isotype	Mouse IgG2a,κ
Clonality	Monoclonal
Clone ID	2D1D10

Examples

Lane 1: 20 µg Hela cell lysate
Lane 2: 20 µg sp2/0 cell lysate
Lane 3: 20 µg goat muscle lysate
Lane 4: 20 µg rabbit muscle lysate
Lane 5: 20 µg chicken muscle lysate
Lane 6: 20 µg CHO cell lysate
Lane 7: 20 µg fish muscle lysate

Primary antibody: 1 µg/ml THE^TM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)

Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000)

Primary antibody:
A. 0.1 μg/ml THE^TM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)
B. 0.1 μg/ml THE^TM Anti-β-actin Monoclonal Antibody (Mouse) (Company A,clone 8226)
Secondary antibody:
Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, 1:10,000, A00160)
The signal was developed with LumiSensor^TM HRP Substrate Kit (GenScript, L00221V500)

Primary antibody:
A. 0.1 μg/ml THE^TM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)
B. 0.1 μg/ml THE^TM Anti-β-actin Monoclonal Antibody (Mouse) (Company A, clone 8226)
Secondary antibody:
Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, 1:10,000, A00160)
The signal was developed with LumiSensor^TM HRP Substrate Kit (GenScript, L00221V500)

Background

Target Background

β-actin is one of the six different actin isoforms that have been identified.The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties.As a consequence, it has been difficult to produce potent antisera against this protein.Therefore, the availability of monoclonal antibodies to ß-actin provides a useful and specific tool in the study of the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.

Synonyms

cytoplasmic 1 antibody;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

THE™ beta Actin Antibody, mAb, Mouse

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