LOC105219193 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105219193
Entrez Gene ID	105219193
Full Name	E3 ubiquitin-protein ligase Su(dx)
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011195168.1	XP_011193470.1	E3 ubiquitin-protein ligase Su(dx)
XM_011195168.2	XP_011193470.1	E3 ubiquitin-protein ligase Su(dx)

Pathways from BioSystems
Gene Ontology

The following LOC105219193 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105219193 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa206451	XM_011195168.2 Latest version!	Zeugodacus cucurbitae E3 ubiquitin-protein ligase Su(dx) (LOC105219193), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
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OBa206451	XM_011195168.1	Bactrocera cucurbitae E3 ubiquitin-protein ligase Su(dx) (LOC105219193), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105219193 ( XM_011195168.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011193470.1 Bactrocera cucurbitae E3 ubiquitin-protein ligase Su(dx) (LOC105219193), mRNA.

Price & Availability↑

CloneID	OBa206451
Clone ID Related Accession (Same CDS sequence)	XM_011195168.1 , XM_011195168.2
Accession Version	XM_011195168.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	E3 ubiquitin-protein ligase Su(dx)
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863929.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATG GAAATGTTAT GTCAGCAGCT TCCATAGGGC ATGCAGCTGC TGACTACTAT 
ACAGTAGCGC CAGCAACAGC GACAGTGTCA TCAACACCAA CACCAATACC AACAGCAATA 
GCAACAGCAA CACAAGCAGC CAGCATGGCT GGTAATGCCG GTGTAGGTGG CGTCATGCAG 
ACCAGTAGTG GCGTTACCGT TTCAACAGTG GGCAGCAGTA TTAACAATGG CAGTAGTAGC 
GGTGGCAATA TGGGTGGTAG CAATATTAAT TTGAATGCGC CCACAATGGC GACTGGGACA 
GCTGTTGCGG TGGCAGCGGG CACAACAACG ACTTGCGGCA GCATGGGCAA CAATCAAGGT 
TATCACCAGT TGAGTGTCAC GATTGAAGAA GCTTCACTGC GCAACAATGG GTTTCTAAAG 
CCTAATCCAT ATGTGGAATT AGTAATCGAT AATAAAAGTA AACGTAAAAC TGATGTGGTG 
AAGAATACTT ATCTACCGAA ATGGAATGAG GAATTCACAG TGCTGGTAAC CCCAAACTCC 
GTGTTACACA TCAAAGTGCT CGATCACTCA AGCTTTCGGA AAGACACGCT ACTCGGCGAG 
CGCATCATCA ATTTGGCGCA TGTTCTGCAA CAATTAAATG GACGTTTCGA GTATCTTGAG 
CTCACGATGG ATTTGCTGGC TACCTCCAAA TCAGATAATC GGCAAACGAA AATTGGCGAG 
CTCGTCGCAG TGCTAAACGG ACTCAAGCTG GACATGCCGA ACGGCGTGCA GCAGCAAAAC 
GGCAATGGTA TGCAAATGCT GCCGCCAACG ATCGGTGGCG ACGGCACGAT ATTCACGCCG 
GCGGCGGGCG TACGTGGACG CATACGCATG CGCAATGCGG AGAATGGCGC GCTTAACGCT 
CAAACAGCGG CGGGTGCGGG TGGTAGTAGC AATGTGGCGC ACTTGACGGT CGGTGGTGGT 
TCGCGCTCAC CCGTTGCGAA TGGCAATCTG GAGCGCTGCG GTAGTGGTGG CAATGTACCG 
AGTGGAGCTG TGCGGCGTGC TGCGCCACCG CTGCCGCCAC CCGTTTGGGA ACAACAACAG 
CCAATGCCAC AAACGCAACA ACAGCAACAA CATGTTGCAC TACAACAACA GCAAATGCAG 
GTGCAACAGC AGCAACAACA ACAGCAATCA CAAATGCAAG TACAACGCAT GCCATCGATG 
AATGGTGGTG GCGGCGCAGT GGCGGTGAAC GCGGCCGGAG TGCAAGTGCG TCCCATGTCG 
CAAATGTATG CGAATGGCGG TGGTGGCGCT ACCAGTATGT CGCCGTTAGC GCTGCCGTCA 
CAACCACAAC AACCGCAAAC TCAACAGCAA CTGATGGATA GCGCACTTTT GAGCGCGGCG 
GGCGATCAGG GTGCGCTGTT GACGCAAGGC GCAATGCCCG TCTCCAATGT GGCCGATCAA 
CAGTTGCAGG CCGCTCAGGC GGACGATGAA CCGCTGCCAG CCGGTTGGGA GATACGCTTC 
GATCAATATG GTCGCCGTTA TTACGTCGAT CACAATACGC GCTCTACATA TTGGGAAAAA 
CCGACGCCGC TACCGCCCGG TTGGGAGATA CGCAAAGATC CACGCGGACG CGTCTACTAT 
GTGGATCACA ATACGCGCAC AACAACCTGG CAGCGACCGA ACAGCGAACG GCTCATGCAT 
TTCCAGCATT GGCAGGGACA GCGTGCGCAT GTGGTGGCGC AGGGTAATCA GCGTTTCCTC 
TACTCACAAC AGCAACAACA ACCAACAGCG GTTACAGCCA CTGTGACGCA AGACGATGAG 
GATGCGCTGG GTCCGCTGCC CGATGGCTGG GAGAAGAAGG TGCAATCAGA TAATCGCGTG 
TACTTTGTGA ATCACAAGAA CCGCACCACA CAGTGGGAGG ATCCGCGCAC GCAAGGTCAA 
GAGGTCAGTC TGATGAATGA GGGTCCATTA CCACCCGGCT GGGAGATACG CTACACCACA 
ACCGGTGAAC GCTTCTTTGT CGATCACAAT ACGCGTCGCA CGACATTCGA AGATCCGCGT 
CCGGGCGCGC CCAAAGGCGC TAAGGGTGTT TATGGTGTGC CGCGCGCTTA TGAGCGCTCC 
TTCCGCTGGA AGCTCTCACA ATTCCGTTAC CTGTGCCAGA GCAACGCATT GCCGTCGCAC 
ATTAAGATAA CGGTGACGCG TCAAACGCTC TTCGAAGATT CATACCACCA GATAATGCGT 
TTGCCCGCCT ACGAACTGCG TCGCCGCCTC TATATTATAT TCCGCGGCGA GGAGGGTCTC 
GACTATGGTG GCGTGTCGCG TGAATGGTTC TTTTTGCTCT CACACGAAGT GCTCAATCCC 
ATGTATTGTC TCTTCGAGTA TGCCAACAAG AATAACTACA GTCTACAAAT CAATCCGGCC 
TCGTATGTGA ATCCCGATCA CTTGCAATAT TTCAAATTTA TTGGTCGCTT CATTGCGATG 
GCGCTGTATC ATGGTCGCTT CATTTACTCC GGTTTTACGA TGCCTTTCTA CAAGCGTATG 
CTGAATAAGA AATTGACCAT AAAAGACATT GAGACCATCG ATCCGGAGTT TTATAATTCT 
TTGATTTGGG TGCGTGATAA TAATATTGAC GAGTGTGGCC TGGAGTTGTG GTTCAGCGTG 
GACTTTGAGG TGCTCGGCCA GATAATACAC CATGAATTGA AGGAGAATGG TGAAAAAGAA 
CGTGTCACCG AAGAGAATAA GGAGGAATAC ATAACCCTCA TGACGGAATG GCGCATGACG 
AGAGGTATCG AACAGCAAAC GAAAACGTTC CTGGAAGGCT TCAACGAAGT CGTGCCGCTA 
GAGTGGCTCA AATATTTCGA TGAACGCGAA CTGGAGCTCA TACTGTGCGG CATGCAGGAT 
GTGGATGTGG ACGATTGGCA GCGCAATACA ATCTATAGGC ATTACAATCG CAATTCCAAG 
CAGGTCATGT GGTTCTGGCA GTTCGTGCGC GATACGGATA ATGAGAAGCG CGCGCGTCTG 
CTGCAATTCG TCACCGGCAC GTGTCGTGTA CCAGTCGGTG GCTTCGCGGA ACTTATGGGC 
TCCAATGGAC CGCAACGTTT CTGCATTGAG AAGGTCGGTA AGGAGACGTG GCTGCCGCGT 
TCGCATACCT GCTTCAATCG GCTGGACTTG CCGCCCTACA AGAGCTACGA TCAGCTGGTG 
GAGAAGTTGA CATTTGCCAT TGAAGAGACT GAGGGCTTCT GCCAAGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011193470.1
CDS	902..4132
Translation	MADGNVMSAASIGHAAADYYTVAPATATVSSTPTPIPTAIATATQAASMAGNAGVGGVMQTSSGVTVSTVGSSINNGSSSGGNMGGSNINLNAPTMATGTAVAVAAGTTTTCGSMGNNQGYHQLSVTIEEASLRNNGFLKPNPYVELVIDNKSKRKTDVVKNTYLPKWNEEFTVLVTPNSVLHIKVLDHSSFRKDTLLGERIINLAHVLQQLNGRFEYLELTMDLLATSKSDNRQTKIGELVAVLNGLKLDMPNGVQQQNGNGMQMLPPTIGGDGTIFTPAAGVRGRIRMRNAENGALNAQTAAGAGGSSNVAHLTVGGGSRSPVANGNLERCGSGGNVPSGAVRRAAPPLPPPVWEQQQPMPQTQQQQQHVALQQQQMQVQQQQQQQQSQMQVQRMPSMNGGGGAVAVNAAGVQVRPMSQMYANGGGGATSMSPLALPSQPQQPQTQQQLMDSALLSAAGDQGALLTQGAMPVSNVADQQLQAAQADDEPLPAGWEIRFDQYGRRYYVDHNTRSTYWEKPTPLPPGWEIRKDPRGRVYYVDHNTRTTTWQRPNSERLMHFQHWQGQRAHVVAQGNQRFLYSQQQQQPTAVTATVTQDDEDALGPLPDGWEKKVQSDNRVYFVNHKNRTTQWEDPRTQGQEVSLMNEGPLPPGWEIRYTTTGERFFVDHNTRRTTFEDPRPGAPKGAKGVYGVPRAYERSFRWKLSQFRYLCQSNALPSHIKITVTRQTLFEDSYHQIMRLPAYELRRRLYIIFRGEEGLDYGGVSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLQYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTIKDIETIDPEFYNSLIWVRDNNIDECGLELWFSVDFEVLGQIIHHELKENGEKERVTEENKEEYITLMTEWRMTRGIEQQTKTFLEGFNEVVPLEWLKYFDERELELILCGMQDVDVDDWQRNTIYRHYNRNSKQVMWFWQFVRDTDNEKRARLLQFVTGTCRVPVGGFAELMGSNGPQRFCIEKVGKETWLPRSHTCFNRLDLPPYKSYDQLVEKLTFAIEETEGFCQE

Target ORF information:

RefSeq Version	XM_011195168.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae E3 ubiquitin-protein ligase Su(dx) (LOC105219193), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011195168.1

ORF Insert Sequence:

ATGGCCGATG GAAATGTTAT GTCAGCAGCT TCCATAGGGC ATGCAGCTGC TGACTACTAT 
ACAGTAGCGC CAGCAACAGC GACAGTGTCA TCAACACCAA CACCAATACC AACAGCAATA 
GCAACAGCAA CACAAGCAGC CAGCATGGCT GGTAATGCCG GTGTAGGTGG CGTCATGCAG 
ACCAGTAGTG GCGTTACCGT TTCAACAGTG GGCAGCAGTA TTAACAATGG CAGTAGTAGC 
GGTGGCAATA TGGGTGGTAG CAATATTAAT TTGAATGCGC CCACAATGGC GACTGGGACA 
GCTGTTGCGG TGGCAGCGGG CACAACAACG ACTTGCGGCA GCATGGGCAA CAATCAAGGT 
TATCACCAGT TGAGTGTCAC GATTGAAGAA GCTTCACTGC GCAACAATGG GTTTCTAAAG 
CCTAATCCAT ATGTGGAATT AGTAATCGAT AATAAAAGTA AACGTAAAAC TGATGTGGTG 
AAGAATACTT ATCTACCGAA ATGGAATGAG GAATTCACAG TGCTGGTAAC CCCAAACTCC 
GTGTTACACA TCAAAGTGCT CGATCACTCA AGCTTTCGGA AAGACACGCT ACTCGGCGAG 
CGCATCATCA ATTTGGCGCA TGTTCTGCAA CAATTAAATG GACGTTTCGA GTATCTTGAG 
CTCACGATGG ATTTGCTGGC TACCTCCAAA TCAGATAATC GGCAAACGAA AATTGGCGAG 
CTCGTCGCAG TGCTAAACGG ACTCAAGCTG GACATGCCGA ACGGCGTGCA GCAGCAAAAC 
GGCAATGGTA TGCAAATGCT GCCGCCAACG ATCGGTGGCG ACGGCACGAT ATTCACGCCG 
GCGGCGGGCG TACGTGGACG CATACGCATG CGCAATGCGG AGAATGGCGC GCTTAACGCT 
CAAACAGCGG CGGGTGCGGG TGGTAGTAGC AATGTGGCGC ACTTGACGGT CGGTGGTGGT 
TCGCGCTCAC CCGTTGCGAA TGGCAATCTG GAGCGCTGCG GTAGTGGTGG CAATGTACCG 
AGTGGAGCTG TGCGGCGTGC TGCGCCACCG CTGCCGCCAC CCGTTTGGGA ACAACAACAG 
CCAATGCCAC AAACGCAACA ACAGCAACAA CATGTTGCAC TACAACAACA GCAAATGCAG 
GTGCAACAGC AGCAACAACA ACAGCAATCA CAAATGCAAG TACAACGCAT GCCATCGATG 
AATGGTGGTG GCGGCGCAGT GGCGGTGAAC GCGGCCGGAG TGCAAGTGCG TCCCATGTCG 
CAAATGTATG CGAATGGCGG TGGTGGCGCT ACCAGTATGT CGCCGTTAGC GCTGCCGTCA 
CAACCACAAC AACCGCAAAC TCAACAGCAA CTGATGGATA GCGCACTTTT GAGCGCGGCG 
GGCGATCAGG GTGCGCTGTT GACGCAAGGC GCAATGCCCG TCTCCAATGT GGCCGATCAA 
CAGTTGCAGG CCGCTCAGGC GGACGATGAA CCGCTGCCAG CCGGTTGGGA GATACGCTTC 
GATCAATATG GTCGCCGTTA TTACGTCGAT CACAATACGC GCTCTACATA TTGGGAAAAA 
CCGACGCCGC TACCGCCCGG TTGGGAGATA CGCAAAGATC CACGCGGACG CGTCTACTAT 
GTGGATCACA ATACGCGCAC AACAACCTGG CAGCGACCGA ACAGCGAACG GCTCATGCAT 
TTCCAGCATT GGCAGGGACA GCGTGCGCAT GTGGTGGCGC AGGGTAATCA GCGTTTCCTC 
TACTCACAAC AGCAACAACA ACCAACAGCG GTTACAGCCA CTGTGACGCA AGACGATGAG 
GATGCGCTGG GTCCGCTGCC CGATGGCTGG GAGAAGAAGG TGCAATCAGA TAATCGCGTG 
TACTTTGTGA ATCACAAGAA CCGCACCACA CAGTGGGAGG ATCCGCGCAC GCAAGGTCAA 
GAGGTCAGTC TGATGAATGA GGGTCCATTA CCACCCGGCT GGGAGATACG CTACACCACA 
ACCGGTGAAC GCTTCTTTGT CGATCACAAT ACGCGTCGCA CGACATTCGA AGATCCGCGT 
CCGGGCGCGC CCAAAGGCGC TAAGGGTGTT TATGGTGTGC CGCGCGCTTA TGAGCGCTCC 
TTCCGCTGGA AGCTCTCACA ATTCCGTTAC CTGTGCCAGA GCAACGCATT GCCGTCGCAC 
ATTAAGATAA CGGTGACGCG TCAAACGCTC TTCGAAGATT CATACCACCA GATAATGCGT 
TTGCCCGCCT ACGAACTGCG TCGCCGCCTC TATATTATAT TCCGCGGCGA GGAGGGTCTC 
GACTATGGTG GCGTGTCGCG TGAATGGTTC TTTTTGCTCT CACACGAAGT GCTCAATCCC 
ATGTATTGTC TCTTCGAGTA TGCCAACAAG AATAACTACA GTCTACAAAT CAATCCGGCC 
TCGTATGTGA ATCCCGATCA CTTGCAATAT TTCAAATTTA TTGGTCGCTT CATTGCGATG 
GCGCTGTATC ATGGTCGCTT CATTTACTCC GGTTTTACGA TGCCTTTCTA CAAGCGTATG 
CTGAATAAGA AATTGACCAT AAAAGACATT GAGACCATCG ATCCGGAGTT TTATAATTCT 
TTGATTTGGG TGCGTGATAA TAATATTGAC GAGTGTGGCC TGGAGTTGTG GTTCAGCGTG 
GACTTTGAGG TGCTCGGCCA GATAATACAC CATGAATTGA AGGAGAATGG TGAAAAAGAA 
CGTGTCACCG AAGAGAATAA GGAGGAATAC ATAACCCTCA TGACGGAATG GCGCATGACG 
AGAGGTATCG AACAGCAAAC GAAAACGTTC CTGGAAGGCT TCAACGAAGT CGTGCCGCTA 
GAGTGGCTCA AATATTTCGA TGAACGCGAA CTGGAGCTCA TACTGTGCGG CATGCAGGAT 
GTGGATGTGG ACGATTGGCA GCGCAATACA ATCTATAGGC ATTACAATCG CAATTCCAAG 
CAGGTCATGT GGTTCTGGCA GTTCGTGCGC GATACGGATA ATGAGAAGCG CGCGCGTCTG 
CTGCAATTCG TCACCGGCAC GTGTCGTGTA CCAGTCGGTG GCTTCGCGGA ACTTATGGGC 
TCCAATGGAC CGCAACGTTT CTGCATTGAG AAGGTCGGTA AGGAGACGTG GCTGCCGCGT 
TCGCATACCT GCTTCAATCG GCTGGACTTG CCGCCCTACA AGAGCTACGA TCAGCTGGTG 
GAGAAGTTGA CATTTGCCAT TGAAGAGACT GAGGGCTTCT GCCAAGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105219193 ( XM_011195168.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011193470.1 Zeugodacus cucurbitae E3 ubiquitin-protein ligase Su(dx) (LOC105219193), mRNA.

Price & Availability↑

CloneID	OBa206451
Clone ID Related Accession (Same CDS sequence)	XM_011195168.1 , XM_011195168.2
Accession Version	XM_011195168.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	E3 ubiquitin-protein ligase Su(dx)
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863929.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011195168.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGATG GAAATGTTAT GTCAGCAGCT TCCATAGGGC ATGCAGCTGC TGACTACTAT 
ACAGTAGCGC CAGCAACAGC GACAGTGTCA TCAACACCAA CACCAATACC AACAGCAATA 
GCAACAGCAA CACAAGCAGC CAGCATGGCT GGTAATGCCG GTGTAGGTGG CGTCATGCAG 
ACCAGTAGTG GCGTTACCGT TTCAACAGTG GGCAGCAGTA TTAACAATGG CAGTAGTAGC 
GGTGGCAATA TGGGTGGTAG CAATATTAAT TTGAATGCGC CCACAATGGC GACTGGGACA 
GCTGTTGCGG TGGCAGCGGG CACAACAACG ACTTGCGGCA GCATGGGCAA CAATCAAGGT 
TATCACCAGT TGAGTGTCAC GATTGAAGAA GCTTCACTGC GCAACAATGG GTTTCTAAAG 
CCTAATCCAT ATGTGGAATT AGTAATCGAT AATAAAAGTA AACGTAAAAC TGATGTGGTG 
AAGAATACTT ATCTACCGAA ATGGAATGAG GAATTCACAG TGCTGGTAAC CCCAAACTCC 
GTGTTACACA TCAAAGTGCT CGATCACTCA AGCTTTCGGA AAGACACGCT ACTCGGCGAG 
CGCATCATCA ATTTGGCGCA TGTTCTGCAA CAATTAAATG GACGTTTCGA GTATCTTGAG 
CTCACGATGG ATTTGCTGGC TACCTCCAAA TCAGATAATC GGCAAACGAA AATTGGCGAG 
CTCGTCGCAG TGCTAAACGG ACTCAAGCTG GACATGCCGA ACGGCGTGCA GCAGCAAAAC 
GGCAATGGTA TGCAAATGCT GCCGCCAACG ATCGGTGGCG ACGGCACGAT ATTCACGCCG 
GCGGCGGGCG TACGTGGACG CATACGCATG CGCAATGCGG AGAATGGCGC GCTTAACGCT 
CAAACAGCGG CGGGTGCGGG TGGTAGTAGC AATGTGGCGC ACTTGACGGT CGGTGGTGGT 
TCGCGCTCAC CCGTTGCGAA TGGCAATCTG GAGCGCTGCG GTAGTGGTGG CAATGTACCG 
AGTGGAGCTG TGCGGCGTGC TGCGCCACCG CTGCCGCCAC CCGTTTGGGA ACAACAACAG 
CCAATGCCAC AAACGCAACA ACAGCAACAA CATGTTGCAC TACAACAACA GCAAATGCAG 
GTGCAACAGC AGCAACAACA ACAGCAATCA CAAATGCAAG TACAACGCAT GCCATCGATG 
AATGGTGGTG GCGGCGCAGT GGCGGTGAAC GCGGCCGGAG TGCAAGTGCG TCCCATGTCG 
CAAATGTATG CGAATGGCGG TGGTGGCGCT ACCAGTATGT CGCCGTTAGC GCTGCCGTCA 
CAACCACAAC AACCGCAAAC TCAACAGCAA CTGATGGATA GCGCACTTTT GAGCGCGGCG 
GGCGATCAGG GTGCGCTGTT GACGCAAGGC GCAATGCCCG TCTCCAATGT GGCCGATCAA 
CAGTTGCAGG CCGCTCAGGC GGACGATGAA CCGCTGCCAG CCGGTTGGGA GATACGCTTC 
GATCAATATG GTCGCCGTTA TTACGTCGAT CACAATACGC GCTCTACATA TTGGGAAAAA 
CCGACGCCGC TACCGCCCGG TTGGGAGATA CGCAAAGATC CACGCGGACG CGTCTACTAT 
GTGGATCACA ATACGCGCAC AACAACCTGG CAGCGACCGA ACAGCGAACG GCTCATGCAT 
TTCCAGCATT GGCAGGGACA GCGTGCGCAT GTGGTGGCGC AGGGTAATCA GCGTTTCCTC 
TACTCACAAC AGCAACAACA ACCAACAGCG GTTACAGCCA CTGTGACGCA AGACGATGAG 
GATGCGCTGG GTCCGCTGCC CGATGGCTGG GAGAAGAAGG TGCAATCAGA TAATCGCGTG 
TACTTTGTGA ATCACAAGAA CCGCACCACA CAGTGGGAGG ATCCGCGCAC GCAAGGTCAA 
GAGGTCAGTC TGATGAATGA GGGTCCATTA CCACCCGGCT GGGAGATACG CTACACCACA 
ACCGGTGAAC GCTTCTTTGT CGATCACAAT ACGCGTCGCA CGACATTCGA AGATCCGCGT 
CCGGGCGCGC CCAAAGGCGC TAAGGGTGTT TATGGTGTGC CGCGCGCTTA TGAGCGCTCC 
TTCCGCTGGA AGCTCTCACA ATTCCGTTAC CTGTGCCAGA GCAACGCATT GCCGTCGCAC 
ATTAAGATAA CGGTGACGCG TCAAACGCTC TTCGAAGATT CATACCACCA GATAATGCGT 
TTGCCCGCCT ACGAACTGCG TCGCCGCCTC TATATTATAT TCCGCGGCGA GGAGGGTCTC 
GACTATGGTG GCGTGTCGCG TGAATGGTTC TTTTTGCTCT CACACGAAGT GCTCAATCCC 
ATGTATTGTC TCTTCGAGTA TGCCAACAAG AATAACTACA GTCTACAAAT CAATCCGGCC 
TCGTATGTGA ATCCCGATCA CTTGCAATAT TTCAAATTTA TTGGTCGCTT CATTGCGATG 
GCGCTGTATC ATGGTCGCTT CATTTACTCC GGTTTTACGA TGCCTTTCTA CAAGCGTATG 
CTGAATAAGA AATTGACCAT AAAAGACATT GAGACCATCG ATCCGGAGTT TTATAATTCT 
TTGATTTGGG TGCGTGATAA TAATATTGAC GAGTGTGGCC TGGAGTTGTG GTTCAGCGTG 
GACTTTGAGG TGCTCGGCCA GATAATACAC CATGAATTGA AGGAGAATGG TGAAAAAGAA 
CGTGTCACCG AAGAGAATAA GGAGGAATAC ATAACCCTCA TGACGGAATG GCGCATGACG 
AGAGGTATCG AACAGCAAAC GAAAACGTTC CTGGAAGGCT TCAACGAAGT CGTGCCGCTA 
GAGTGGCTCA AATATTTCGA TGAACGCGAA CTGGAGCTCA TACTGTGCGG CATGCAGGAT 
GTGGATGTGG ACGATTGGCA GCGCAATACA ATCTATAGGC ATTACAATCG CAATTCCAAG 
CAGGTCATGT GGTTCTGGCA GTTCGTGCGC GATACGGATA ATGAGAAGCG CGCGCGTCTG 
CTGCAATTCG TCACCGGCAC GTGTCGTGTA CCAGTCGGTG GCTTCGCGGA ACTTATGGGC 
TCCAATGGAC CGCAACGTTT CTGCATTGAG AAGGTCGGTA AGGAGACGTG GCTGCCGCGT 
TCGCATACCT GCTTCAATCG GCTGGACTTG CCGCCCTACA AGAGCTACGA TCAGCTGGTG 
GAGAAGTTGA CATTTGCCAT TGAAGAGACT GAGGGCTTCT GCCAAGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011193470.1
CDS	931..4161
Translation	MADGNVMSAASIGHAAADYYTVAPATATVSSTPTPIPTAIATATQAASMAGNAGVGGVMQTSSGVTVSTVGSSINNGSSSGGNMGGSNINLNAPTMATGTAVAVAAGTTTTCGSMGNNQGYHQLSVTIEEASLRNNGFLKPNPYVELVIDNKSKRKTDVVKNTYLPKWNEEFTVLVTPNSVLHIKVLDHSSFRKDTLLGERIINLAHVLQQLNGRFEYLELTMDLLATSKSDNRQTKIGELVAVLNGLKLDMPNGVQQQNGNGMQMLPPTIGGDGTIFTPAAGVRGRIRMRNAENGALNAQTAAGAGGSSNVAHLTVGGGSRSPVANGNLERCGSGGNVPSGAVRRAAPPLPPPVWEQQQPMPQTQQQQQHVALQQQQMQVQQQQQQQQSQMQVQRMPSMNGGGGAVAVNAAGVQVRPMSQMYANGGGGATSMSPLALPSQPQQPQTQQQLMDSALLSAAGDQGALLTQGAMPVSNVADQQLQAAQADDEPLPAGWEIRFDQYGRRYYVDHNTRSTYWEKPTPLPPGWEIRKDPRGRVYYVDHNTRTTTWQRPNSERLMHFQHWQGQRAHVVAQGNQRFLYSQQQQQPTAVTATVTQDDEDALGPLPDGWEKKVQSDNRVYFVNHKNRTTQWEDPRTQGQEVSLMNEGPLPPGWEIRYTTTGERFFVDHNTRRTTFEDPRPGAPKGAKGVYGVPRAYERSFRWKLSQFRYLCQSNALPSHIKITVTRQTLFEDSYHQIMRLPAYELRRRLYIIFRGEEGLDYGGVSREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLQYFKFIGRFIAMALYHGRFIYSGFTMPFYKRMLNKKLTIKDIETIDPEFYNSLIWVRDNNIDECGLELWFSVDFEVLGQIIHHELKENGEKERVTEENKEEYITLMTEWRMTRGIEQQTKTFLEGFNEVVPLEWLKYFDERELELILCGMQDVDVDDWQRNTIYRHYNRNSKQVMWFWQFVRDTDNEKRARLLQFVTGTCRVPVGGFAELMGSNGPQRFCIEKVGKETWLPRSHTCFNRLDLPPYKSYDQLVEKLTFAIEETEGFCQE

Target ORF information:

RefSeq Version	XM_011195168.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae E3 ubiquitin-protein ligase Su(dx) (LOC105219193), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011195168.2

ORF Insert Sequence:

ATGGCCGATG GAAATGTTAT GTCAGCAGCT TCCATAGGGC ATGCAGCTGC TGACTACTAT 
ACAGTAGCGC CAGCAACAGC GACAGTGTCA TCAACACCAA CACCAATACC AACAGCAATA 
GCAACAGCAA CACAAGCAGC CAGCATGGCT GGTAATGCCG GTGTAGGTGG CGTCATGCAG 
ACCAGTAGTG GCGTTACCGT TTCAACAGTG GGCAGCAGTA TTAACAATGG CAGTAGTAGC 
GGTGGCAATA TGGGTGGTAG CAATATTAAT TTGAATGCGC CCACAATGGC GACTGGGACA 
GCTGTTGCGG TGGCAGCGGG CACAACAACG ACTTGCGGCA GCATGGGCAA CAATCAAGGT 
TATCACCAGT TGAGTGTCAC GATTGAAGAA GCTTCACTGC GCAACAATGG GTTTCTAAAG 
CCTAATCCAT ATGTGGAATT AGTAATCGAT AATAAAAGTA AACGTAAAAC TGATGTGGTG 
AAGAATACTT ATCTACCGAA ATGGAATGAG GAATTCACAG TGCTGGTAAC CCCAAACTCC 
GTGTTACACA TCAAAGTGCT CGATCACTCA AGCTTTCGGA AAGACACGCT ACTCGGCGAG 
CGCATCATCA ATTTGGCGCA TGTTCTGCAA CAATTAAATG GACGTTTCGA GTATCTTGAG 
CTCACGATGG ATTTGCTGGC TACCTCCAAA TCAGATAATC GGCAAACGAA AATTGGCGAG 
CTCGTCGCAG TGCTAAACGG ACTCAAGCTG GACATGCCGA ACGGCGTGCA GCAGCAAAAC 
GGCAATGGTA TGCAAATGCT GCCGCCAACG ATCGGTGGCG ACGGCACGAT ATTCACGCCG 
GCGGCGGGCG TACGTGGACG CATACGCATG CGCAATGCGG AGAATGGCGC GCTTAACGCT 
CAAACAGCGG CGGGTGCGGG TGGTAGTAGC AATGTGGCGC ACTTGACGGT CGGTGGTGGT 
TCGCGCTCAC CCGTTGCGAA TGGCAATCTG GAGCGCTGCG GTAGTGGTGG CAATGTACCG 
AGTGGAGCTG TGCGGCGTGC TGCGCCACCG CTGCCGCCAC CCGTTTGGGA ACAACAACAG 
CCAATGCCAC AAACGCAACA ACAGCAACAA CATGTTGCAC TACAACAACA GCAAATGCAG 
GTGCAACAGC AGCAACAACA ACAGCAATCA CAAATGCAAG TACAACGCAT GCCATCGATG 
AATGGTGGTG GCGGCGCAGT GGCGGTGAAC GCGGCCGGAG TGCAAGTGCG TCCCATGTCG 
CAAATGTATG CGAATGGCGG TGGTGGCGCT ACCAGTATGT CGCCGTTAGC GCTGCCGTCA 
CAACCACAAC AACCGCAAAC TCAACAGCAA CTGATGGATA GCGCACTTTT GAGCGCGGCG 
GGCGATCAGG GTGCGCTGTT GACGCAAGGC GCAATGCCCG TCTCCAATGT GGCCGATCAA 
CAGTTGCAGG CCGCTCAGGC GGACGATGAA CCGCTGCCAG CCGGTTGGGA GATACGCTTC 
GATCAATATG GTCGCCGTTA TTACGTCGAT CACAATACGC GCTCTACATA TTGGGAAAAA 
CCGACGCCGC TACCGCCCGG TTGGGAGATA CGCAAAGATC CACGCGGACG CGTCTACTAT 
GTGGATCACA ATACGCGCAC AACAACCTGG CAGCGACCGA ACAGCGAACG GCTCATGCAT 
TTCCAGCATT GGCAGGGACA GCGTGCGCAT GTGGTGGCGC AGGGTAATCA GCGTTTCCTC 
TACTCACAAC AGCAACAACA ACCAACAGCG GTTACAGCCA CTGTGACGCA AGACGATGAG 
GATGCGCTGG GTCCGCTGCC CGATGGCTGG GAGAAGAAGG TGCAATCAGA TAATCGCGTG 
TACTTTGTGA ATCACAAGAA CCGCACCACA CAGTGGGAGG ATCCGCGCAC GCAAGGTCAA 
GAGGTCAGTC TGATGAATGA GGGTCCATTA CCACCCGGCT GGGAGATACG CTACACCACA 
ACCGGTGAAC GCTTCTTTGT CGATCACAAT ACGCGTCGCA CGACATTCGA AGATCCGCGT 
CCGGGCGCGC CCAAAGGCGC TAAGGGTGTT TATGGTGTGC CGCGCGCTTA TGAGCGCTCC 
TTCCGCTGGA AGCTCTCACA ATTCCGTTAC CTGTGCCAGA GCAACGCATT GCCGTCGCAC 
ATTAAGATAA CGGTGACGCG TCAAACGCTC TTCGAAGATT CATACCACCA GATAATGCGT 
TTGCCCGCCT ACGAACTGCG TCGCCGCCTC TATATTATAT TCCGCGGCGA GGAGGGTCTC 
GACTATGGTG GCGTGTCGCG TGAATGGTTC TTTTTGCTCT CACACGAAGT GCTCAATCCC 
ATGTATTGTC TCTTCGAGTA TGCCAACAAG AATAACTACA GTCTACAAAT CAATCCGGCC 
TCGTATGTGA ATCCCGATCA CTTGCAATAT TTCAAATTTA TTGGTCGCTT CATTGCGATG 
GCGCTGTATC ATGGTCGCTT CATTTACTCC GGTTTTACGA TGCCTTTCTA CAAGCGTATG 
CTGAATAAGA AATTGACCAT AAAAGACATT GAGACCATCG ATCCGGAGTT TTATAATTCT 
TTGATTTGGG TGCGTGATAA TAATATTGAC GAGTGTGGCC TGGAGTTGTG GTTCAGCGTG 
GACTTTGAGG TGCTCGGCCA GATAATACAC CATGAATTGA AGGAGAATGG TGAAAAAGAA 
CGTGTCACCG AAGAGAATAA GGAGGAATAC ATAACCCTCA TGACGGAATG GCGCATGACG 
AGAGGTATCG AACAGCAAAC GAAAACGTTC CTGGAAGGCT TCAACGAAGT CGTGCCGCTA 
GAGTGGCTCA AATATTTCGA TGAACGCGAA CTGGAGCTCA TACTGTGCGG CATGCAGGAT 
GTGGATGTGG ACGATTGGCA GCGCAATACA ATCTATAGGC ATTACAATCG CAATTCCAAG 
CAGGTCATGT GGTTCTGGCA GTTCGTGCGC GATACGGATA ATGAGAAGCG CGCGCGTCTG 
CTGCAATTCG TCACCGGCAC GTGTCGTGTA CCAGTCGGTG GCTTCGCGGA ACTTATGGGC 
TCCAATGGAC CGCAACGTTT CTGCATTGAG AAGGTCGGTA AGGAGACGTG GCTGCCGCGT 
TCGCATACCT GCTTCAATCG GCTGGACTTG CCGCCCTACA AGAGCTACGA TCAGCTGGTG 
GAGAAGTTGA CATTTGCCAT TGAAGAGACT GAGGGCTTCT GCCAAGAGTA G