LOC105217665 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105217665
Entrez Gene ID	105217665
Full Name	C-mannosyltransferase dpy-19 homolog
Synonyms	CG6659
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011192784.1	XP_011191086.1	C-mannosyltransferase dpy-19 homolog
XM_011192784.2	XP_011191086.1	C-mannosyltransferase dpy-19 homolog

Pathways from BioSystems
Gene Ontology

The following LOC105217665 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105217665 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa204720	XM_011192784.2 Latest version!	Zeugodacus cucurbitae C-mannosyltransferase dpy-19 homolog (LOC105217665), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OBa204720	XM_011192784.1	Bactrocera cucurbitae C-mannosyltransferase dpy-19 homolog (LOC105217665), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105217665 ( XM_011192784.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011191086.1 Bactrocera cucurbitae C-mannosyltransferase dpy-19 homolog (LOC105217665), mRNA.

Price & Availability↑

CloneID	OBa204720
Clone ID Related Accession (Same CDS sequence)	XM_011192784.1 , XM_011192784.2
Accession Version	XM_011192784.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	C-mannosyltransferase dpy-19 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863825.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAC AAAAATTGTT ACTTATTCTC TCGCAGTCAT TAATTGGCAC CGGCTTCTTC 
TTTTTGTATG TTCAACATGT GAGGTCAATA TTTGAGTCCC GCACCAATAT TGCATATTTA 
ACACAATTAG AACGGGAGTC TTTGCTGCGG CGGGAGGACT CGCTCTATTA TTCATTTTAT 
AAGACACTTA CAGAAGAAGC TGATTTTTGG AGTGGTTACG ATAAGCTACA AAATTTAACT 
GGCATTGAAT ATCCACAAAA TGTGAACGTA ATACAAAGGT TCCATGTTTT ACCCGAATTG 
ACAATAGGAT ATCTCTACCA TTTGCTAAGG CGATATGCTT TCACAGAAAG TTTTCCAATA 
TTTAGCTGTT GGCGCACCAA CGACGTGCGT CTGCCACTGG AGCATCGTCA TTGTGACGGC 
ATTGGACAAC CCATGCAGTT TTACTTAGAA TGTGTATGGC TTCTTGGTGG GGTCACTGTA 
CTAGTTATTT ATATATACGG GACTTTACTA AGTGAAAATA TATTTGGCGG CATTTACGCA 
GTGGTTTCTT ATGTTATGTT CCACAGTTTT GCGTCCAAGA TTTATGATTC CCCTATGGCA 
CGTGAAAATT TTGCCTTTCC AATTATTTTC ATGCAAATGT TTTACCTTTG CTTGTGCATC 
GACCGGATCA CGGAGCGCAA AGTCTATCAC ATGCGACTTT TTATAACTAT TAAATTGACC 
CTGCTGACAG CTATTGCTCT TCTCTGTTGG CAGTTTTCTT CTGTTATTTT CGCAACGCAA 
GTTCTTATTA TGATGGCTCC TTGGACGCCA AGCTCAATAT CTGAGGTGAT TTCTGGTAGT 
TTTACGATGG ATTATGCGCT CTCGCAACTT ATTGCCACAT CACTAGCGTA CTACTGTTCC 
TTCAGCAACA AGAAATACAT TTTCGCTTGG CATATCGGTC CAGCAATCGG ACTTCTCTTT 
CTCAGTATGG AGCGGCAAGT TAAACCTCAA TCGCCAAATA GTGGCATTTC GTTCAAAACC 
ATTTGGGCGG GCTTATTGGC AGCAGTTAGT GTACAGGGAA CTTTATATGA CATGTTGGAG 
AGAACAGGAT TTGCACGTTC AGTGGATAAC GGGTTCGCCT TGTATCGTGA TTTAATAGTG 
CAATGGTGCT TCCAGGCGAA AGTGAGTTTC ACCACCAGCA TTGCGGCATG CAATCCATCT 
TATCAAAATT TGAATATTGC CCATTTGTGG GAGCTTATAA AGACCCTAAT TGTGAAGCCA 
TATTGTATGT ACGGTGTAGT GATGTTGGCG AAGTTTTTTC GCAAGTGGCG CAAGGGTAGT 
GAAAAGAAAA CCCCCAGCAA AGATAATGTG GAACGGGCCA AAAAATATGT GCTCGAAGAT 
TTCTTGGAAG AGAATCACAT TTCAATGGTT GATATGTCAA ATAAAGAGAC TGAGAACGAT 
TTAAATGAAT GCCTCAAATT ACTGGAAGAA TGCGACTATG ACTACAATCG CTATAAACAA 
GAGAGAACAA AGTCGAAAAA CAACAAGGGC GTTGAACACG AAGAGTTCAT GAATAACATA 
AAATTACTAA AAGAACAAAT TAAAGAGGGT AACCAACGAA GAGATCACGA TAATCGGAAT 
ATGTCTAAAA GTAATCATGA TGAGAATGAA CAAGAGCAAC AAGAAGAAAG TACTAAAGGA 
GATGTAAAAC CGTCCACAAG TGAGGATAAA GCTACAATAA ACAGCAGTTC GATCCCAGAA 
GCGCCAAAGG CAGAAATAAC CGATTCAGTG AATAATACGA ATAGCGGCAC ATCCACGAAT 
GCTGGTGAAA TGCCGAGTAG TTTAAAAAGT GGCGATGCCA ATAAAAACAC ACGACGTTCA 
CGGTATAAAG GATCGGATTG TGCGGCTTCA AAAGCTGATT ATCACAGCGA TATTTTCAGC 
TTCCACTATG TCTACAGCTT TGTACAAATG GCCGTTTTTA CGGCTTTAGG GCTTAGTATT 
AAGAAATTAT TCTTCCTATG TTTCACTCAA GGTTGTGTCA TTGCGCCGAC AATATGCTCC 
AAATTTTGGT ACCGCAAACA ACGCAATATA TTTTGGGCAG TATCGTTGGC GGTTTTCTTA 
ACCAGCATGG TCAATCCCGG TTTAGTGAAT ATACGTGAAG AATACTTCCC TACACGCCAA 
GGGCACGCTG GCGATGATTT GGAAAGCATG TTGGAATGGA TCAAAATTAA TACGGAACGC 
GACGCTGTTT TTGCAGGTCC GGTAGACATC ATTGGCACGG TGCATTTAGT TACTAGACGA 
CCGATTGTGA ACCACGCCCA TTTGGAAATA CGTCACATCC ATGAACGTAC CGAACATGTG 
TACTCAGTGT TCAGCCGTCA ACAGTCTTCG GATATTTACA ATCAATACTC CCAACTTAAA 
GTGCAATATT TAATTATATC GCTCCAAGAC TGCAGCAATG ACATTCGTGA TGACTGTGAC 
TTGCTTTCGA TTTGGGATGA CCTGCAGCCG TCATATCGCA AATATCCGCA ATTTTGTTCG 
GAATTGGCAA AGAAGAATAT ACCTAGCTTT TTGAAAGTAT TCTCCAATGA TTATTATGGT 
ATTATTAAGA TGTTTTCGCA GAGTGTACAA ATCAATTTGA AGCTCAGTAA GATGCCCGAA 
AAAGTAATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011191086.1
CDS	217..2868
Translation	MKEQKLLLILSQSLIGTGFFFLYVQHVRSIFESRTNIAYLTQLERESLLRREDSLYYSFYKTLTEEADFWSGYDKLQNLTGIEYPQNVNVIQRFHVLPELTIGYLYHLLRRYAFTESFPIFSCWRTNDVRLPLEHRHCDGIGQPMQFYLECVWLLGGVTVLVIYIYGTLLSENIFGGIYAVVSYVMFHSFASKIYDSPMARENFAFPIIFMQMFYLCLCIDRITERKVYHMRLFITIKLTLLTAIALLCWQFSSVIFATQVLIMMAPWTPSSISEVISGSFTMDYALSQLIATSLAYYCSFSNKKYIFAWHIGPAIGLLFLSMERQVKPQSPNSGISFKTIWAGLLAAVSVQGTLYDMLERTGFARSVDNGFALYRDLIVQWCFQAKVSFTTSIAACNPSYQNLNIAHLWELIKTLIVKPYCMYGVVMLAKFFRKWRKGSEKKTPSKDNVERAKKYVLEDFLEENHISMVDMSNKETENDLNECLKLLEECDYDYNRYKQERTKSKNNKGVEHEEFMNNIKLLKEQIKEGNQRRDHDNRNMSKSNHDENEQEQQEESTKGDVKPSTSEDKATINSSSIPEAPKAEITDSVNNTNSGTSTNAGEMPSSLKSGDANKNTRRSRYKGSDCAASKADYHSDIFSFHYVYSFVQMAVFTALGLSIKKLFFLCFTQGCVIAPTICSKFWYRKQRNIFWAVSLAVFLTSMVNPGLVNIREEYFPTRQGHAGDDLESMLEWIKINTERDAVFAGPVDIIGTVHLVTRRPIVNHAHLEIRHIHERTEHVYSVFSRQQSSDIYNQYSQLKVQYLIISLQDCSNDIRDDCDLLSIWDDLQPSYRKYPQFCSELAKKNIPSFLKVFSNDYYGIIKMFSQSVQINLKLSKMPEKVM

Target ORF information:

RefSeq Version	XM_011192784.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae C-mannosyltransferase dpy-19 homolog (LOC105217665), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011192784.1

ORF Insert Sequence:

ATGAAGGAAC AAAAATTGTT ACTTATTCTC TCGCAGTCAT TAATTGGCAC CGGCTTCTTC 
TTTTTGTATG TTCAACATGT GAGGTCAATA TTTGAGTCCC GCACCAATAT TGCATATTTA 
ACACAATTAG AACGGGAGTC TTTGCTGCGG CGGGAGGACT CGCTCTATTA TTCATTTTAT 
AAGACACTTA CAGAAGAAGC TGATTTTTGG AGTGGTTACG ATAAGCTACA AAATTTAACT 
GGCATTGAAT ATCCACAAAA TGTGAACGTA ATACAAAGGT TCCATGTTTT ACCCGAATTG 
ACAATAGGAT ATCTCTACCA TTTGCTAAGG CGATATGCTT TCACAGAAAG TTTTCCAATA 
TTTAGCTGTT GGCGCACCAA CGACGTGCGT CTGCCACTGG AGCATCGTCA TTGTGACGGC 
ATTGGACAAC CCATGCAGTT TTACTTAGAA TGTGTATGGC TTCTTGGTGG GGTCACTGTA 
CTAGTTATTT ATATATACGG GACTTTACTA AGTGAAAATA TATTTGGCGG CATTTACGCA 
GTGGTTTCTT ATGTTATGTT CCACAGTTTT GCGTCCAAGA TTTATGATTC CCCTATGGCA 
CGTGAAAATT TTGCCTTTCC AATTATTTTC ATGCAAATGT TTTACCTTTG CTTGTGCATC 
GACCGGATCA CGGAGCGCAA AGTCTATCAC ATGCGACTTT TTATAACTAT TAAATTGACC 
CTGCTGACAG CTATTGCTCT TCTCTGTTGG CAGTTTTCTT CTGTTATTTT CGCAACGCAA 
GTTCTTATTA TGATGGCTCC TTGGACGCCA AGCTCAATAT CTGAGGTGAT TTCTGGTAGT 
TTTACGATGG ATTATGCGCT CTCGCAACTT ATTGCCACAT CACTAGCGTA CTACTGTTCC 
TTCAGCAACA AGAAATACAT TTTCGCTTGG CATATCGGTC CAGCAATCGG ACTTCTCTTT 
CTCAGTATGG AGCGGCAAGT TAAACCTCAA TCGCCAAATA GTGGCATTTC GTTCAAAACC 
ATTTGGGCGG GCTTATTGGC AGCAGTTAGT GTACAGGGAA CTTTATATGA CATGTTGGAG 
AGAACAGGAT TTGCACGTTC AGTGGATAAC GGGTTCGCCT TGTATCGTGA TTTAATAGTG 
CAATGGTGCT TCCAGGCGAA AGTGAGTTTC ACCACCAGCA TTGCGGCATG CAATCCATCT 
TATCAAAATT TGAATATTGC CCATTTGTGG GAGCTTATAA AGACCCTAAT TGTGAAGCCA 
TATTGTATGT ACGGTGTAGT GATGTTGGCG AAGTTTTTTC GCAAGTGGCG CAAGGGTAGT 
GAAAAGAAAA CCCCCAGCAA AGATAATGTG GAACGGGCCA AAAAATATGT GCTCGAAGAT 
TTCTTGGAAG AGAATCACAT TTCAATGGTT GATATGTCAA ATAAAGAGAC TGAGAACGAT 
TTAAATGAAT GCCTCAAATT ACTGGAAGAA TGCGACTATG ACTACAATCG CTATAAACAA 
GAGAGAACAA AGTCGAAAAA CAACAAGGGC GTTGAACACG AAGAGTTCAT GAATAACATA 
AAATTACTAA AAGAACAAAT TAAAGAGGGT AACCAACGAA GAGATCACGA TAATCGGAAT 
ATGTCTAAAA GTAATCATGA TGAGAATGAA CAAGAGCAAC AAGAAGAAAG TACTAAAGGA 
GATGTAAAAC CGTCCACAAG TGAGGATAAA GCTACAATAA ACAGCAGTTC GATCCCAGAA 
GCGCCAAAGG CAGAAATAAC CGATTCAGTG AATAATACGA ATAGCGGCAC ATCCACGAAT 
GCTGGTGAAA TGCCGAGTAG TTTAAAAAGT GGCGATGCCA ATAAAAACAC ACGACGTTCA 
CGGTATAAAG GATCGGATTG TGCGGCTTCA AAAGCTGATT ATCACAGCGA TATTTTCAGC 
TTCCACTATG TCTACAGCTT TGTACAAATG GCCGTTTTTA CGGCTTTAGG GCTTAGTATT 
AAGAAATTAT TCTTCCTATG TTTCACTCAA GGTTGTGTCA TTGCGCCGAC AATATGCTCC 
AAATTTTGGT ACCGCAAACA ACGCAATATA TTTTGGGCAG TATCGTTGGC GGTTTTCTTA 
ACCAGCATGG TCAATCCCGG TTTAGTGAAT ATACGTGAAG AATACTTCCC TACACGCCAA 
GGGCACGCTG GCGATGATTT GGAAAGCATG TTGGAATGGA TCAAAATTAA TACGGAACGC 
GACGCTGTTT TTGCAGGTCC GGTAGACATC ATTGGCACGG TGCATTTAGT TACTAGACGA 
CCGATTGTGA ACCACGCCCA TTTGGAAATA CGTCACATCC ATGAACGTAC CGAACATGTG 
TACTCAGTGT TCAGCCGTCA ACAGTCTTCG GATATTTACA ATCAATACTC CCAACTTAAA 
GTGCAATATT TAATTATATC GCTCCAAGAC TGCAGCAATG ACATTCGTGA TGACTGTGAC 
TTGCTTTCGA TTTGGGATGA CCTGCAGCCG TCATATCGCA AATATCCGCA ATTTTGTTCG 
GAATTGGCAA AGAAGAATAT ACCTAGCTTT TTGAAAGTAT TCTCCAATGA TTATTATGGT 
ATTATTAAGA TGTTTTCGCA GAGTGTACAA ATCAATTTGA AGCTCAGTAA GATGCCCGAA 
AAAGTAATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217665 ( XM_011192784.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011191086.1 Zeugodacus cucurbitae C-mannosyltransferase dpy-19 homolog (LOC105217665), mRNA.

Price & Availability↑

CloneID	OBa204720
Clone ID Related Accession (Same CDS sequence)	XM_011192784.1 , XM_011192784.2
Accession Version	XM_011192784.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	C-mannosyltransferase dpy-19 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863825.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011192784.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAC AAAAATTGTT ACTTATTCTC TCGCAGTCAT TAATTGGCAC CGGCTTCTTC 
TTTTTGTATG TTCAACATGT GAGGTCAATA TTTGAGTCCC GCACCAATAT TGCATATTTA 
ACACAATTAG AACGGGAGTC TTTGCTGCGG CGGGAGGACT CGCTCTATTA TTCATTTTAT 
AAGACACTTA CAGAAGAAGC TGATTTTTGG AGTGGTTACG ATAAGCTACA AAATTTAACT 
GGCATTGAAT ATCCACAAAA TGTGAACGTA ATACAAAGGT TCCATGTTTT ACCCGAATTG 
ACAATAGGAT ATCTCTACCA TTTGCTAAGG CGATATGCTT TCACAGAAAG TTTTCCAATA 
TTTAGCTGTT GGCGCACCAA CGACGTGCGT CTGCCACTGG AGCATCGTCA TTGTGACGGC 
ATTGGACAAC CCATGCAGTT TTACTTAGAA TGTGTATGGC TTCTTGGTGG GGTCACTGTA 
CTAGTTATTT ATATATACGG GACTTTACTA AGTGAAAATA TATTTGGCGG CATTTACGCA 
GTGGTTTCTT ATGTTATGTT CCACAGTTTT GCGTCCAAGA TTTATGATTC CCCTATGGCA 
CGTGAAAATT TTGCCTTTCC AATTATTTTC ATGCAAATGT TTTACCTTTG CTTGTGCATC 
GACCGGATCA CGGAGCGCAA AGTCTATCAC ATGCGACTTT TTATAACTAT TAAATTGACC 
CTGCTGACAG CTATTGCTCT TCTCTGTTGG CAGTTTTCTT CTGTTATTTT CGCAACGCAA 
GTTCTTATTA TGATGGCTCC TTGGACGCCA AGCTCAATAT CTGAGGTGAT TTCTGGTAGT 
TTTACGATGG ATTATGCGCT CTCGCAACTT ATTGCCACAT CACTAGCGTA CTACTGTTCC 
TTCAGCAACA AGAAATACAT TTTCGCTTGG CATATCGGTC CAGCAATCGG ACTTCTCTTT 
CTCAGTATGG AGCGGCAAGT TAAACCTCAA TCGCCAAATA GTGGCATTTC GTTCAAAACC 
ATTTGGGCGG GCTTATTGGC AGCAGTTAGT GTACAGGGAA CTTTATATGA CATGTTGGAG 
AGAACAGGAT TTGCACGTTC AGTGGATAAC GGGTTCGCCT TGTATCGTGA TTTAATAGTG 
CAATGGTGCT TCCAGGCGAA AGTGAGTTTC ACCACCAGCA TTGCGGCATG CAATCCATCT 
TATCAAAATT TGAATATTGC CCATTTGTGG GAGCTTATAA AGACCCTAAT TGTGAAGCCA 
TATTGTATGT ACGGTGTAGT GATGTTGGCG AAGTTTTTTC GCAAGTGGCG CAAGGGTAGT 
GAAAAGAAAA CCCCCAGCAA AGATAATGTG GAACGGGCCA AAAAATATGT GCTCGAAGAT 
TTCTTGGAAG AGAATCACAT TTCAATGGTT GATATGTCAA ATAAAGAGAC TGAGAACGAT 
TTAAATGAAT GCCTCAAATT ACTGGAAGAA TGCGACTATG ACTACAATCG CTATAAACAA 
GAGAGAACAA AGTCGAAAAA CAACAAGGGC GTTGAACACG AAGAGTTCAT GAATAACATA 
AAATTACTAA AAGAACAAAT TAAAGAGGGT AACCAACGAA GAGATCACGA TAATCGGAAT 
ATGTCTAAAA GTAATCATGA TGAGAATGAA CAAGAGCAAC AAGAAGAAAG TACTAAAGGA 
GATGTAAAAC CGTCCACAAG TGAGGATAAA GCTACAATAA ACAGCAGTTC GATCCCAGAA 
GCGCCAAAGG CAGAAATAAC CGATTCAGTG AATAATACGA ATAGCGGCAC ATCCACGAAT 
GCTGGTGAAA TGCCGAGTAG TTTAAAAAGT GGCGATGCCA ATAAAAACAC ACGACGTTCA 
CGGTATAAAG GATCGGATTG TGCGGCTTCA AAAGCTGATT ATCACAGCGA TATTTTCAGC 
TTCCACTATG TCTACAGCTT TGTACAAATG GCCGTTTTTA CGGCTTTAGG GCTTAGTATT 
AAGAAATTAT TCTTCCTATG TTTCACTCAA GGTTGTGTCA TTGCGCCGAC AATATGCTCC 
AAATTTTGGT ACCGCAAACA ACGCAATATA TTTTGGGCAG TATCGTTGGC GGTTTTCTTA 
ACCAGCATGG TCAATCCCGG TTTAGTGAAT ATACGTGAAG AATACTTCCC TACACGCCAA 
GGGCACGCTG GCGATGATTT GGAAAGCATG TTGGAATGGA TCAAAATTAA TACGGAACGC 
GACGCTGTTT TTGCAGGTCC GGTAGACATC ATTGGCACGG TGCATTTAGT TACTAGACGA 
CCGATTGTGA ACCACGCCCA TTTGGAAATA CGTCACATCC ATGAACGTAC CGAACATGTG 
TACTCAGTGT TCAGCCGTCA ACAGTCTTCG GATATTTACA ATCAATACTC CCAACTTAAA 
GTGCAATATT TAATTATATC GCTCCAAGAC TGCAGCAATG ACATTCGTGA TGACTGTGAC 
TTGCTTTCGA TTTGGGATGA CCTGCAGCCG TCATATCGCA AATATCCGCA ATTTTGTTCG 
GAATTGGCAA AGAAGAATAT ACCTAGCTTT TTGAAAGTAT TCTCCAATGA TTATTATGGT 
ATTATTAAGA TGTTTTCGCA GAGTGTACAA ATCAATTTGA AGCTCAGTAA GATGCCCGAA 
AAAGTAATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011191086.1
CDS	214..2865
Translation	MKEQKLLLILSQSLIGTGFFFLYVQHVRSIFESRTNIAYLTQLERESLLRREDSLYYSFYKTLTEEADFWSGYDKLQNLTGIEYPQNVNVIQRFHVLPELTIGYLYHLLRRYAFTESFPIFSCWRTNDVRLPLEHRHCDGIGQPMQFYLECVWLLGGVTVLVIYIYGTLLSENIFGGIYAVVSYVMFHSFASKIYDSPMARENFAFPIIFMQMFYLCLCIDRITERKVYHMRLFITIKLTLLTAIALLCWQFSSVIFATQVLIMMAPWTPSSISEVISGSFTMDYALSQLIATSLAYYCSFSNKKYIFAWHIGPAIGLLFLSMERQVKPQSPNSGISFKTIWAGLLAAVSVQGTLYDMLERTGFARSVDNGFALYRDLIVQWCFQAKVSFTTSIAACNPSYQNLNIAHLWELIKTLIVKPYCMYGVVMLAKFFRKWRKGSEKKTPSKDNVERAKKYVLEDFLEENHISMVDMSNKETENDLNECLKLLEECDYDYNRYKQERTKSKNNKGVEHEEFMNNIKLLKEQIKEGNQRRDHDNRNMSKSNHDENEQEQQEESTKGDVKPSTSEDKATINSSSIPEAPKAEITDSVNNTNSGTSTNAGEMPSSLKSGDANKNTRRSRYKGSDCAASKADYHSDIFSFHYVYSFVQMAVFTALGLSIKKLFFLCFTQGCVIAPTICSKFWYRKQRNIFWAVSLAVFLTSMVNPGLVNIREEYFPTRQGHAGDDLESMLEWIKINTERDAVFAGPVDIIGTVHLVTRRPIVNHAHLEIRHIHERTEHVYSVFSRQQSSDIYNQYSQLKVQYLIISLQDCSNDIRDDCDLLSIWDDLQPSYRKYPQFCSELAKKNIPSFLKVFSNDYYGIIKMFSQSVQINLKLSKMPEKVM

Target ORF information:

RefSeq Version	XM_011192784.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae C-mannosyltransferase dpy-19 homolog (LOC105217665), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011192784.2

ORF Insert Sequence:

ATGAAGGAAC AAAAATTGTT ACTTATTCTC TCGCAGTCAT TAATTGGCAC CGGCTTCTTC 
TTTTTGTATG TTCAACATGT GAGGTCAATA TTTGAGTCCC GCACCAATAT TGCATATTTA 
ACACAATTAG AACGGGAGTC TTTGCTGCGG CGGGAGGACT CGCTCTATTA TTCATTTTAT 
AAGACACTTA CAGAAGAAGC TGATTTTTGG AGTGGTTACG ATAAGCTACA AAATTTAACT 
GGCATTGAAT ATCCACAAAA TGTGAACGTA ATACAAAGGT TCCATGTTTT ACCCGAATTG 
ACAATAGGAT ATCTCTACCA TTTGCTAAGG CGATATGCTT TCACAGAAAG TTTTCCAATA 
TTTAGCTGTT GGCGCACCAA CGACGTGCGT CTGCCACTGG AGCATCGTCA TTGTGACGGC 
ATTGGACAAC CCATGCAGTT TTACTTAGAA TGTGTATGGC TTCTTGGTGG GGTCACTGTA 
CTAGTTATTT ATATATACGG GACTTTACTA AGTGAAAATA TATTTGGCGG CATTTACGCA 
GTGGTTTCTT ATGTTATGTT CCACAGTTTT GCGTCCAAGA TTTATGATTC CCCTATGGCA 
CGTGAAAATT TTGCCTTTCC AATTATTTTC ATGCAAATGT TTTACCTTTG CTTGTGCATC 
GACCGGATCA CGGAGCGCAA AGTCTATCAC ATGCGACTTT TTATAACTAT TAAATTGACC 
CTGCTGACAG CTATTGCTCT TCTCTGTTGG CAGTTTTCTT CTGTTATTTT CGCAACGCAA 
GTTCTTATTA TGATGGCTCC TTGGACGCCA AGCTCAATAT CTGAGGTGAT TTCTGGTAGT 
TTTACGATGG ATTATGCGCT CTCGCAACTT ATTGCCACAT CACTAGCGTA CTACTGTTCC 
TTCAGCAACA AGAAATACAT TTTCGCTTGG CATATCGGTC CAGCAATCGG ACTTCTCTTT 
CTCAGTATGG AGCGGCAAGT TAAACCTCAA TCGCCAAATA GTGGCATTTC GTTCAAAACC 
ATTTGGGCGG GCTTATTGGC AGCAGTTAGT GTACAGGGAA CTTTATATGA CATGTTGGAG 
AGAACAGGAT TTGCACGTTC AGTGGATAAC GGGTTCGCCT TGTATCGTGA TTTAATAGTG 
CAATGGTGCT TCCAGGCGAA AGTGAGTTTC ACCACCAGCA TTGCGGCATG CAATCCATCT 
TATCAAAATT TGAATATTGC CCATTTGTGG GAGCTTATAA AGACCCTAAT TGTGAAGCCA 
TATTGTATGT ACGGTGTAGT GATGTTGGCG AAGTTTTTTC GCAAGTGGCG CAAGGGTAGT 
GAAAAGAAAA CCCCCAGCAA AGATAATGTG GAACGGGCCA AAAAATATGT GCTCGAAGAT 
TTCTTGGAAG AGAATCACAT TTCAATGGTT GATATGTCAA ATAAAGAGAC TGAGAACGAT 
TTAAATGAAT GCCTCAAATT ACTGGAAGAA TGCGACTATG ACTACAATCG CTATAAACAA 
GAGAGAACAA AGTCGAAAAA CAACAAGGGC GTTGAACACG AAGAGTTCAT GAATAACATA 
AAATTACTAA AAGAACAAAT TAAAGAGGGT AACCAACGAA GAGATCACGA TAATCGGAAT 
ATGTCTAAAA GTAATCATGA TGAGAATGAA CAAGAGCAAC AAGAAGAAAG TACTAAAGGA 
GATGTAAAAC CGTCCACAAG TGAGGATAAA GCTACAATAA ACAGCAGTTC GATCCCAGAA 
GCGCCAAAGG CAGAAATAAC CGATTCAGTG AATAATACGA ATAGCGGCAC ATCCACGAAT 
GCTGGTGAAA TGCCGAGTAG TTTAAAAAGT GGCGATGCCA ATAAAAACAC ACGACGTTCA 
CGGTATAAAG GATCGGATTG TGCGGCTTCA AAAGCTGATT ATCACAGCGA TATTTTCAGC 
TTCCACTATG TCTACAGCTT TGTACAAATG GCCGTTTTTA CGGCTTTAGG GCTTAGTATT 
AAGAAATTAT TCTTCCTATG TTTCACTCAA GGTTGTGTCA TTGCGCCGAC AATATGCTCC 
AAATTTTGGT ACCGCAAACA ACGCAATATA TTTTGGGCAG TATCGTTGGC GGTTTTCTTA 
ACCAGCATGG TCAATCCCGG TTTAGTGAAT ATACGTGAAG AATACTTCCC TACACGCCAA 
GGGCACGCTG GCGATGATTT GGAAAGCATG TTGGAATGGA TCAAAATTAA TACGGAACGC 
GACGCTGTTT TTGCAGGTCC GGTAGACATC ATTGGCACGG TGCATTTAGT TACTAGACGA 
CCGATTGTGA ACCACGCCCA TTTGGAAATA CGTCACATCC ATGAACGTAC CGAACATGTG 
TACTCAGTGT TCAGCCGTCA ACAGTCTTCG GATATTTACA ATCAATACTC CCAACTTAAA 
GTGCAATATT TAATTATATC GCTCCAAGAC TGCAGCAATG ACATTCGTGA TGACTGTGAC 
TTGCTTTCGA TTTGGGATGA CCTGCAGCCG TCATATCGCA AATATCCGCA ATTTTGTTCG 
GAATTGGCAA AGAAGAATAT ACCTAGCTTT TTGAAAGTAT TCTCCAATGA TTATTATGGT 
ATTATTAAGA TGTTTTCGCA GAGTGTACAA ATCAATTTGA AGCTCAGTAA GATGCCCGAA 
AAAGTAATGT AG