LOC105217148 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105217148
Entrez Gene ID	105217148
Full Name	endoplasmic reticulum metallopeptidase 1
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011192011.2	XP_011190313.1	endoplasmic reticulum metallopeptidase 1
XM_011192012.2	XP_011190314.1	endoplasmic reticulum metallopeptidase 1
XM_029043443.1	XP_028899276.1	endoplasmic reticulum metallopeptidase 1
XM_029043444.1	XP_028899277.1	endoplasmic reticulum metallopeptidase 1
XM_029043445.1	XP_028899278.1	endoplasmic reticulum metallopeptidase 1
XM_011192011.1	XP_011190313.1	endoplasmic reticulum metallopeptidase 1
XM_011192012.1	XP_011190314.1	endoplasmic reticulum metallopeptidase 1

Pathways from BioSystems
Gene Ontology

The following LOC105217148 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105217148 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa204169	XM_029043445.1 Latest version!	Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa204169	XM_029043443.1 Latest version!	Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa204169	XM_011192011.2 Latest version!	Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa204169	XM_011192012.2 Latest version!	Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa204169	XM_029043444.1 Latest version!	Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBa204169	XM_011192011.1	Bactrocera cucurbitae endoplasmic reticulum metallopeptidase 1 (LOC105217148), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa204169	XM_011192012.1	Bactrocera cucurbitae endoplasmic reticulum metallopeptidase 1 (LOC105217148), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105217148 ( XM_011192011.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011190313.1 Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa204169
Clone ID Related Accession (Same CDS sequence)	XM_011192011.2 , XM_011192012.2 , XM_029043443.1 , XM_029043444.1 , XM_029043445.1 , XM_011192011.1 , XM_011192012.1
Accession Version	XM_011192011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	endoplasmic reticulum metallopeptidase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011192011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011190313.1
CDS	567..3155
Translation	MGFKSKYHIDVDFEVPKKLNWYYAPLFTAFWLILYLAVVLTQVVRLPTPLMLNDEAAHPDSYIAERAEQILVKLGRLGPKVVGSEANEVKAVELLVGEINKVKAQMSDYFELEIDVQVVTGSYVHWTMVNMYQGVQNVVAKLSAKNNTSENYVLINSHFDSVPDSPGAGDDNSMVTTMLEVLRVIAKSDGPLKHPIVFLFNGAEENPLQASHGFITQHKWAKNCKALINLDAAGSGGRELLFQSGPGNPWLMNYYRRVPHPFANTLGEELFQNDFIPSDTDFRIFRDYGGVPGLDMAYTYNGYVYHTEFDRFNILPKGSLQNTGDNVLHLARSIANAPEMENPTVNAAGHVIFYDFLGWFILFYSLNTSIIVNSVVCAIAVCAIGVSLFFMSARSGLGWLAIIRRYALAFAIQLLSLSVGAAIAVIIALFMDGVGRSMTWFSQTWLICGLYFCPIFFCMGFFPALFLERTKKDLLSLGFRIQLFMHSHCLLLILLTIFLTAFNVRSAFMFMLAVLFDIAALIINLITKWHRKAYWFAIAVMICQVLPFIYYTSTAHEIYFTMIPMMGRSGSASNPDIIIAAIAVFFGCLFAGFIMPLYMFFRKTRTIMLCFLGVTVVFIILAVTPVGFPYTPKVAVQRYSLLHAKRVLHNADGSERLNESGVYIYSQDRRTHMADDNIKSIGVKHKVSDICNDELYCGLPIYNHRWHKAKEHSFWIPTDEEPNIPGENPKLTLTAKTDGDTANTSRYSFTLTGPDHMTIFIGPKAPAKIVKWSFNDTMIRDNWEPPYFIYYSYGKDNSPLDFYIDLETTSVNANTPNLEIGIGGHYIHQTRIRTDGYEKFVQSFPNYAYVSDWPAFYESWLF

Target ORF information:

RefSeq Version	XM_011192011.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011192011.2

ORF Insert Sequence:

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217148 ( XM_011192012.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011190314.1 Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa204169
Clone ID Related Accession (Same CDS sequence)	XM_011192011.2 , XM_011192012.2 , XM_029043443.1 , XM_029043444.1 , XM_029043445.1 , XM_011192011.1 , XM_011192012.1
Accession Version	XM_011192012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	endoplasmic reticulum metallopeptidase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011192012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011190314.1
CDS	504..3092
Translation	MGFKSKYHIDVDFEVPKKLNWYYAPLFTAFWLILYLAVVLTQVVRLPTPLMLNDEAAHPDSYIAERAEQILVKLGRLGPKVVGSEANEVKAVELLVGEINKVKAQMSDYFELEIDVQVVTGSYVHWTMVNMYQGVQNVVAKLSAKNNTSENYVLINSHFDSVPDSPGAGDDNSMVTTMLEVLRVIAKSDGPLKHPIVFLFNGAEENPLQASHGFITQHKWAKNCKALINLDAAGSGGRELLFQSGPGNPWLMNYYRRVPHPFANTLGEELFQNDFIPSDTDFRIFRDYGGVPGLDMAYTYNGYVYHTEFDRFNILPKGSLQNTGDNVLHLARSIANAPEMENPTVNAAGHVIFYDFLGWFILFYSLNTSIIVNSVVCAIAVCAIGVSLFFMSARSGLGWLAIIRRYALAFAIQLLSLSVGAAIAVIIALFMDGVGRSMTWFSQTWLICGLYFCPIFFCMGFFPALFLERTKKDLLSLGFRIQLFMHSHCLLLILLTIFLTAFNVRSAFMFMLAVLFDIAALIINLITKWHRKAYWFAIAVMICQVLPFIYYTSTAHEIYFTMIPMMGRSGSASNPDIIIAAIAVFFGCLFAGFIMPLYMFFRKTRTIMLCFLGVTVVFIILAVTPVGFPYTPKVAVQRYSLLHAKRVLHNADGSERLNESGVYIYSQDRRTHMADDNIKSIGVKHKVSDICNDELYCGLPIYNHRWHKAKEHSFWIPTDEEPNIPGENPKLTLTAKTDGDTANTSRYSFTLTGPDHMTIFIGPKAPAKIVKWSFNDTMIRDNWEPPYFIYYSYGKDNSPLDFYIDLETTSVNANTPNLEIGIGGHYIHQTRIRTDGYEKFVQSFPNYAYVSDWPAFYESWLF

Target ORF information:

RefSeq Version	XM_011192012.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011192012.2

ORF Insert Sequence:

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217148 ( XM_029043443.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028899276.1 Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa204169
Clone ID Related Accession (Same CDS sequence)	XM_011192011.2 , XM_011192012.2 , XM_029043443.1 , XM_029043444.1 , XM_029043445.1 , XM_011192011.1 , XM_011192012.1
Accession Version	XM_029043443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	endoplasmic reticulum metallopeptidase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028899276.1
CDS	520..3108
Translation	MGFKSKYHIDVDFEVPKKLNWYYAPLFTAFWLILYLAVVLTQVVRLPTPLMLNDEAAHPDSYIAERAEQILVKLGRLGPKVVGSEANEVKAVELLVGEINKVKAQMSDYFELEIDVQVVTGSYVHWTMVNMYQGVQNVVAKLSAKNNTSENYVLINSHFDSVPDSPGAGDDNSMVTTMLEVLRVIAKSDGPLKHPIVFLFNGAEENPLQASHGFITQHKWAKNCKALINLDAAGSGGRELLFQSGPGNPWLMNYYRRVPHPFANTLGEELFQNDFIPSDTDFRIFRDYGGVPGLDMAYTYNGYVYHTEFDRFNILPKGSLQNTGDNVLHLARSIANAPEMENPTVNAAGHVIFYDFLGWFILFYSLNTSIIVNSVVCAIAVCAIGVSLFFMSARSGLGWLAIIRRYALAFAIQLLSLSVGAAIAVIIALFMDGVGRSMTWFSQTWLICGLYFCPIFFCMGFFPALFLERTKKDLLSLGFRIQLFMHSHCLLLILLTIFLTAFNVRSAFMFMLAVLFDIAALIINLITKWHRKAYWFAIAVMICQVLPFIYYTSTAHEIYFTMIPMMGRSGSASNPDIIIAAIAVFFGCLFAGFIMPLYMFFRKTRTIMLCFLGVTVVFIILAVTPVGFPYTPKVAVQRYSLLHAKRVLHNADGSERLNESGVYIYSQDRRTHMADDNIKSIGVKHKVSDICNDELYCGLPIYNHRWHKAKEHSFWIPTDEEPNIPGENPKLTLTAKTDGDTANTSRYSFTLTGPDHMTIFIGPKAPAKIVKWSFNDTMIRDNWEPPYFIYYSYGKDNSPLDFYIDLETTSVNANTPNLEIGIGGHYIHQTRIRTDGYEKFVQSFPNYAYVSDWPAFYESWLF

Target ORF information:

RefSeq Version	XM_029043443.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029043443.1

ORF Insert Sequence:

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217148 ( XM_029043444.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028899277.1 Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa204169
Clone ID Related Accession (Same CDS sequence)	XM_011192011.2 , XM_011192012.2 , XM_029043443.1 , XM_029043444.1 , XM_029043445.1 , XM_011192011.1 , XM_011192012.1
Accession Version	XM_029043444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	endoplasmic reticulum metallopeptidase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028899277.1
CDS	532..3120
Translation	MGFKSKYHIDVDFEVPKKLNWYYAPLFTAFWLILYLAVVLTQVVRLPTPLMLNDEAAHPDSYIAERAEQILVKLGRLGPKVVGSEANEVKAVELLVGEINKVKAQMSDYFELEIDVQVVTGSYVHWTMVNMYQGVQNVVAKLSAKNNTSENYVLINSHFDSVPDSPGAGDDNSMVTTMLEVLRVIAKSDGPLKHPIVFLFNGAEENPLQASHGFITQHKWAKNCKALINLDAAGSGGRELLFQSGPGNPWLMNYYRRVPHPFANTLGEELFQNDFIPSDTDFRIFRDYGGVPGLDMAYTYNGYVYHTEFDRFNILPKGSLQNTGDNVLHLARSIANAPEMENPTVNAAGHVIFYDFLGWFILFYSLNTSIIVNSVVCAIAVCAIGVSLFFMSARSGLGWLAIIRRYALAFAIQLLSLSVGAAIAVIIALFMDGVGRSMTWFSQTWLICGLYFCPIFFCMGFFPALFLERTKKDLLSLGFRIQLFMHSHCLLLILLTIFLTAFNVRSAFMFMLAVLFDIAALIINLITKWHRKAYWFAIAVMICQVLPFIYYTSTAHEIYFTMIPMMGRSGSASNPDIIIAAIAVFFGCLFAGFIMPLYMFFRKTRTIMLCFLGVTVVFIILAVTPVGFPYTPKVAVQRYSLLHAKRVLHNADGSERLNESGVYIYSQDRRTHMADDNIKSIGVKHKVSDICNDELYCGLPIYNHRWHKAKEHSFWIPTDEEPNIPGENPKLTLTAKTDGDTANTSRYSFTLTGPDHMTIFIGPKAPAKIVKWSFNDTMIRDNWEPPYFIYYSYGKDNSPLDFYIDLETTSVNANTPNLEIGIGGHYIHQTRIRTDGYEKFVQSFPNYAYVSDWPAFYESWLF

Target ORF information:

RefSeq Version	XM_029043444.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029043444.1

ORF Insert Sequence:

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217148 ( XM_029043445.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028899278.1 Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa204169
Clone ID Related Accession (Same CDS sequence)	XM_011192011.2 , XM_011192012.2 , XM_029043443.1 , XM_029043444.1 , XM_029043445.1 , XM_011192011.1 , XM_011192012.1
Accession Version	XM_029043445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	endoplasmic reticulum metallopeptidase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028899278.1
CDS	433..3021
Translation	MGFKSKYHIDVDFEVPKKLNWYYAPLFTAFWLILYLAVVLTQVVRLPTPLMLNDEAAHPDSYIAERAEQILVKLGRLGPKVVGSEANEVKAVELLVGEINKVKAQMSDYFELEIDVQVVTGSYVHWTMVNMYQGVQNVVAKLSAKNNTSENYVLINSHFDSVPDSPGAGDDNSMVTTMLEVLRVIAKSDGPLKHPIVFLFNGAEENPLQASHGFITQHKWAKNCKALINLDAAGSGGRELLFQSGPGNPWLMNYYRRVPHPFANTLGEELFQNDFIPSDTDFRIFRDYGGVPGLDMAYTYNGYVYHTEFDRFNILPKGSLQNTGDNVLHLARSIANAPEMENPTVNAAGHVIFYDFLGWFILFYSLNTSIIVNSVVCAIAVCAIGVSLFFMSARSGLGWLAIIRRYALAFAIQLLSLSVGAAIAVIIALFMDGVGRSMTWFSQTWLICGLYFCPIFFCMGFFPALFLERTKKDLLSLGFRIQLFMHSHCLLLILLTIFLTAFNVRSAFMFMLAVLFDIAALIINLITKWHRKAYWFAIAVMICQVLPFIYYTSTAHEIYFTMIPMMGRSGSASNPDIIIAAIAVFFGCLFAGFIMPLYMFFRKTRTIMLCFLGVTVVFIILAVTPVGFPYTPKVAVQRYSLLHAKRVLHNADGSERLNESGVYIYSQDRRTHMADDNIKSIGVKHKVSDICNDELYCGLPIYNHRWHKAKEHSFWIPTDEEPNIPGENPKLTLTAKTDGDTANTSRYSFTLTGPDHMTIFIGPKAPAKIVKWSFNDTMIRDNWEPPYFIYYSYGKDNSPLDFYIDLETTSVNANTPNLEIGIGGHYIHQTRIRTDGYEKFVQSFPNYAYVSDWPAFYESWLF

Target ORF information:

RefSeq Version	XM_029043445.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae endoplasmic reticulum metallopeptidase 1 (Ermp1_2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029043445.1

ORF Insert Sequence:

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217148 ( XM_011192011.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011190313.1 Bactrocera cucurbitae endoplasmic reticulum metallopeptidase 1 (LOC105217148), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa204169
Clone ID Related Accession (Same CDS sequence)	XM_011192011.2 , XM_011192012.2 , XM_029043443.1 , XM_029043444.1 , XM_029043445.1 , XM_011192011.1 , XM_011192012.1
Accession Version	XM_011192011.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	endoplasmic reticulum metallopeptidase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011190313.1
CDS	790..3378
Translation	MGFKSKYHIDVDFEVPKKLNWYYAPLFTAFWLILYLAVVLTQVVRLPTPLMLNDEAAHPDSYIAERAEQILVKLGRLGPKVVGSEANEVKAVELLVGEINKVKAQMSDYFELEIDVQVVTGSYVHWTMVNMYQGVQNVVAKLSAKNNTSENYVLINSHFDSVPDSPGAGDDNSMVTTMLEVLRVIAKSDGPLKHPIVFLFNGAEENPLQASHGFITQHKWAKNCKALINLDAAGSGGRELLFQSGPGNPWLMNYYRRVPHPFANTLGEELFQNDFIPSDTDFRIFRDYGGVPGLDMAYTYNGYVYHTEFDRFNILPKGSLQNTGDNVLHLARSIANAPEMENPTVNAAGHVIFYDFLGWFILFYSLNTSIIVNSVVCAIAVCAIGVSLFFMSARSGLGWLAIIRRYALAFAIQLLSLSVGAAIAVIIALFMDGVGRSMTWFSQTWLICGLYFCPIFFCMGFFPALFLERTKKDLLSLGFRIQLFMHSHCLLLILLTIFLTAFNVRSAFMFMLAVLFDIAALIINLITKWHRKAYWFAIAVMICQVLPFIYYTSTAHEIYFTMIPMMGRSGSASNPDIIIAAIAVFFGCLFAGFIMPLYMFFRKTRTIMLCFLGVTVVFIILAVTPVGFPYTPKVAVQRYSLLHAKRVLHNADGSERLNESGVYIYSQDRRTHMADDNIKSIGVKHKVSDICNDELYCGLPIYNHRWHKAKEHSFWIPTDEEPNIPGENPKLTLTAKTDGDTANTSRYSFTLTGPDHMTIFIGPKAPAKIVKWSFNDTMIRDNWEPPYFIYYSYGKDNSPLDFYIDLETTSVNANTPNLEIGIGGHYIHQTRIRTDGYEKFVQSFPNYAYVSDWPAFYESWLF

Target ORF information:

RefSeq Version	XM_011192011.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae endoplasmic reticulum metallopeptidase 1 (LOC105217148), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011192011.1

ORF Insert Sequence:

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217148 ( XM_011192012.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011190314.1 Bactrocera cucurbitae endoplasmic reticulum metallopeptidase 1 (LOC105217148), transcript variant X2, mRNA.

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CloneID	OBa204169
Clone ID Related Accession (Same CDS sequence)	XM_011192011.2 , XM_011192012.2 , XM_029043443.1 , XM_029043444.1 , XM_029043445.1 , XM_011192011.1 , XM_011192012.1
Accession Version	XM_011192012.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	endoplasmic reticulum metallopeptidase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011190314.1
CDS	674..3262
Translation	MGFKSKYHIDVDFEVPKKLNWYYAPLFTAFWLILYLAVVLTQVVRLPTPLMLNDEAAHPDSYIAERAEQILVKLGRLGPKVVGSEANEVKAVELLVGEINKVKAQMSDYFELEIDVQVVTGSYVHWTMVNMYQGVQNVVAKLSAKNNTSENYVLINSHFDSVPDSPGAGDDNSMVTTMLEVLRVIAKSDGPLKHPIVFLFNGAEENPLQASHGFITQHKWAKNCKALINLDAAGSGGRELLFQSGPGNPWLMNYYRRVPHPFANTLGEELFQNDFIPSDTDFRIFRDYGGVPGLDMAYTYNGYVYHTEFDRFNILPKGSLQNTGDNVLHLARSIANAPEMENPTVNAAGHVIFYDFLGWFILFYSLNTSIIVNSVVCAIAVCAIGVSLFFMSARSGLGWLAIIRRYALAFAIQLLSLSVGAAIAVIIALFMDGVGRSMTWFSQTWLICGLYFCPIFFCMGFFPALFLERTKKDLLSLGFRIQLFMHSHCLLLILLTIFLTAFNVRSAFMFMLAVLFDIAALIINLITKWHRKAYWFAIAVMICQVLPFIYYTSTAHEIYFTMIPMMGRSGSASNPDIIIAAIAVFFGCLFAGFIMPLYMFFRKTRTIMLCFLGVTVVFIILAVTPVGFPYTPKVAVQRYSLLHAKRVLHNADGSERLNESGVYIYSQDRRTHMADDNIKSIGVKHKVSDICNDELYCGLPIYNHRWHKAKEHSFWIPTDEEPNIPGENPKLTLTAKTDGDTANTSRYSFTLTGPDHMTIFIGPKAPAKIVKWSFNDTMIRDNWEPPYFIYYSYGKDNSPLDFYIDLETTSVNANTPNLEIGIGGHYIHQTRIRTDGYEKFVQSFPNYAYVSDWPAFYESWLF

Target ORF information:

RefSeq Version	XM_011192012.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae endoplasmic reticulum metallopeptidase 1 (LOC105217148), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011192012.1

ORF Insert Sequence:

ATGGGTTTCA AATCCAAATA CCACATCGAT GTCGATTTTG AGGTGCCGAA AAAACTCAAT 
TGGTATTATG CGCCACTTTT CACCGCTTTC TGGTTGATAC TCTACTTGGC CGTAGTGCTC 
ACACAAGTTG TACGTCTACC CACACCGCTT ATGCTGAATG ATGAGGCCGC ACATCCCGAC 
TCCTATATAG CCGAACGTGC TGAACAAATT CTAGTCAAGC TAGGCCGCCT CGGTCCTAAG 
GTCGTGGGCA GTGAAGCGAA TGAGGTAAAA GCCGTCGAGC TATTGGTCGG CGAAATCAAT 
AAGGTTAAGG CACAAATGAG CGATTACTTC GAACTCGAAA TCGATGTACA AGTCGTGACC 
GGTTCATATG TACATTGGAC CATGGTGAAT ATGTATCAGG GTGTACAAAA TGTGGTGGCC 
AAATTGAGCG CGAAAAACAA TACAAGCGAA AATTATGTAC TCATAAATTC GCACTTTGAC 
AGCGTGCCTG ATAGTCCGGG TGCTGGTGAT GATAATTCGA TGGTGACAAC AATGCTGGAG 
GTGTTGCGAG TTATCGCCAA ATCAGATGGA CCACTTAAAC ATCCAATTGT ATTTCTCTTC 
AACGGTGCTG AAGAGAATCC GCTGCAGGCA TCACACGGTT TTATAACACA ACATAAATGG 
GCCAAGAATT GCAAAGCCTT AATAAATTTG GATGCTGCTG GTTCAGGAGG CCGCGAATTG 
CTCTTCCAGT CCGGACCTGG TAATCCATGG CTCATGAACT ATTATCGTCG AGTGCCACAT 
CCTTTTGCCA ACACCTTGGG CGAGGAACTG TTTCAGAATG ACTTTATACC ATCCGACACA 
GATTTTCGTA TTTTCCGTGA TTATGGTGGT GTACCAGGTT TGGATATGGC TTATACCTAC 
AATGGTTATG TCTATCACAC GGAGTTTGAT CGCTTCAACA TCCTACCGAA GGGTTCACTA 
CAAAATACCG GCGATAATGT CTTACACTTG GCCAGATCTA TAGCTAACGC ACCGGAAATG 
GAAAATCCAA CAGTCAATGC TGCTGGACAT GTTATTTTCT ACGATTTCCT CGGTTGGTTC 
ATACTCTTCT ACTCGCTAAA CACCAGCATC ATCGTGAACA GTGTGGTCTG TGCGATAGCT 
GTTTGTGCCA TAGGTGTCTC GCTCTTCTTC ATGTCAGCAC GTTCGGGTCT TGGCTGGTTG 
GCGATTATAC GCCGCTATGC ACTCGCATTT GCCATACAAC TACTTTCACT CTCAGTGGGT 
GCAGCTATTG CCGTAATTAT TGCATTATTT ATGGATGGCG TTGGACGCTC TATGACGTGG 
TTTAGTCAAA CATGGTTAAT TTGTGGTCTC TACTTTTGTC CAATATTTTT CTGCATGGGC 
TTCTTTCCGG CATTGTTCTT GGAGCGTACA AAAAAGGATC TGTTGAGCTT GGGCTTCCGC 
ATTCAACTTT TCATGCATTC ACACTGTCTA TTGCTAATTT TGCTAACGAT CTTCCTTACC 
GCATTCAATG TGCGTTCGGC TTTCATGTTT ATGCTTGCTG TGCTATTTGA CATCGCAGCT 
CTGATCATTA ACCTGATTAC AAAATGGCAT CGTAAAGCTT ATTGGTTCGC CATTGCCGTG 
ATGATTTGCC AAGTCTTGCC GTTCATATAC TATACCTCAA CAGCACATGA AATTTACTTT 
ACGATGATAC CGATGATGGG ACGTTCAGGT TCAGCTTCTA ACCCGGATAT TATTATTGCT 
GCTATAGCGG TCTTCTTTGG ATGCCTATTC GCTGGGTTCA TAATGCCACT CTATATGTTC 
TTCCGCAAAA CGCGTACGAT CATGCTTTGT TTCTTGGGCG TCACAGTAGT CTTCATAATA 
CTAGCGGTCA CACCAGTTGG TTTTCCATAT ACACCGAAGG TCGCGGTGCA ACGTTATTCG 
CTACTCCATG CGAAACGTGT ACTACATAAT GCCGATGGCA GTGAACGCCT CAATGAATCG 
GGCGTTTATA TATATTCGCA GGACAGACGT ACACACATGG CGGATGACAA CATAAAAAGT 
ATTGGCGTAA AACATAAAGT CAGTGACATC TGTAATGATG AGCTCTACTG TGGCCTCCCC 
ATATACAATC ATCGTTGGCA CAAGGCGAAA GAACACAGCT TTTGGATACC CACCGATGAG 
GAGCCCAATA TACCTGGCGA AAACCCTAAA CTAACGCTGA CAGCCAAAAC TGATGGGGAC 
ACAGCCAATA CAAGCCGTTA TAGCTTTACG CTCACCGGTC CCGATCACAT GACAATATTT 
ATTGGCCCAA AAGCACCAGC TAAAATTGTT AAATGGTCTT TCAACGATAC AATGATACGT 
GATAATTGGG AACCACCCTA CTTCATCTAC TACTCATATG GCAAAGATAA CAGTCCATTA 
GATTTCTACA TTGATTTGGA GACTACAAGC GTTAACGCGA ACACACCAAA TTTGGAAATC 
GGCATAGGTG GCCATTATAT ACATCAGACA CGCATACGTA CCGATGGTTA TGAAAAGTTC 
GTGCAAAGCT TCCCGAATTA TGCTTATGTA TCAGACTGGC CGGCTTTCTA TGAAAGTTGG 
CTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC105217148 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム