LOC105214345 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105214345
Entrez Gene ID	105214345
Full Name	dorsal-ventral patterning protein tolloid
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011187715.1	XP_011186017.1	dorsal-ventral patterning protein tolloid
XM_011187715.2	XP_011186017.2	dorsal-ventral patterning protein tolloid

Pathways from BioSystems
Gene Ontology

The following LOC105214345 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105214345 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa200980	XM_011187715.1 Latest version!	Bactrocera cucurbitae dorsal-ventral patterning protein tolloid (LOC105214345), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBa256417	XM_011187715.2 Latest version!	Zeugodacus cucurbitae dorsal-ventral patterning protein tolloid (LOC105214345), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105214345 ( XM_011187715.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011186017.1 Bactrocera cucurbitae dorsal-ventral patterning protein tolloid (LOC105214345), mRNA.

Price & Availability↑

CloneID	OBa200980
Clone ID Related Accession (Same CDS sequence)	XM_011187715.1
Accession Version	XM_011187715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	dorsal-ventral patterning protein tolloid
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863728.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGAG ACGACACAAG CGGCATCCCA CGCCAGCGGC TACAGAGCTT GGCATTTCGA 
AGGCGTTTAT TTAAACAACT TAAAGGCAAA AGGGTACCGG TCCACAAAAA GAAGCACCAC 
AAGAGACGTA TTTTTCAGTC TCCCAATGAA GCGAAAAGCA GGCACAACAA GGTCAAATCA 
AGTGTCAATC ATAACCCTAA TCTAGACTCA TTCAACCACT TTAGAAACAC TCCATATTTA 
AGTTCAGTAC CTCGTTCACG GCGTGCAGTT ACCGCCAAAA AGGAACGCAT TTGGGATTAC 
GGTGTCATAC CGTATACCAT CGACGAGATA TTCAGCGGGG TACACAAGGC GCTCTTCATG 
AGAGCCATGC GCCATTGGGA GAATTCCACA TGTATTAAAT TTGTTGAACG CGATGCTAAG 
GTGCACCCTA ATTACATACA TTTTACTGTT AAAAATTGTG GTTGCTGCTC GTTTGTGGGC 
AAGCGCGGTT CCGGACGACA AGCCATTTCG ATTGGTCGCA ACTGTGAAAA ATTCGGAATT 
GTCGTGCATG AATTGGGACA TGTTATTGGG TTTTGGCACG AACATACGCG CACCGACCGA 
GATAAGCATA TATCTATAAA TAAGGAGAAT ATCATGAAGG GGCAGGAGTA TAATTTCGAT 
GTGCTTTCAC CGGAGGATGT TGACTCACTG GGTCTACCAT ATGATTATAA TTCAATCATG 
CATTACGCAA AGAATACTTT TGCCAAGAAT GTTTATTTGG AGACCATTCA GCCTATTGGA 
CTATCGAAGT CCCAACATAT AGAGATCGGC CAAAGATTAC GTCTAAGTAC GGGGGATATA 
ATTAAGGCGA ATCGATTATA TAAATGCAGC AGTTGCGGAC GTACATTCCA AGAGAATTCT 
GGAGAGATAA TCTCACCTCA TTACGAATAC TCAGCATATT CGGCGATACA AGACAGTAAT 
GTGATAGAAG ACAGCGGAAG CGGTGATTAC GAGGTCAGTG ATTTTGACAA ATCTCTAGAG 
AAATGCGAAT GGCGTATCAC AGCAACAAAT GGGGAACGTA TTATTTTGCA AATTCATCAA 
GTGCATCTTC TGCAGTCCAC TAACTGTGCT GTGGATTATT TAGAAATACG AGACGGTTAT 
TGGTACAAAT CTCCTGTTAT TAAGAGACTA TGCGGCAATG TCACGGAGGA AACGGTTAGG 
AGCAGCTCCA GTCGAATGCT CATCAGTTAT GTTAATCACC ACGCAGTCAG AGGATACCGT 
GGATTTTCGG CCAATTTCGA GGTTACATGT GGTGGTGACA TTTTTCTCAC CGAAAGTAGA 
CGTGTTGACT CACCCAATTA TCCATTGGAA TATCTGCCGG ATCGTGAATG CATTTGGCGC 
ATATCGGCAC CGGAGAACCG TCAAGTTGGG CTGAAATTTC AGTCATTCGA ATTGGAGAAT 
CATGATAACT GTGCTTACGA CTATGTGGAA ATACGCGATG GTAATGACAG TGACTCTCAA 
TTAATTGGAA TTTACTGTGG ATATATTCTA CCACCAAATA TCAAGTCTAA TTCAAATCAA 
ATGTTTGTAA AGTTTGTTTC CGATGGTTCG GTCCAGAAGG TTGGCTTTTC CGCGGTCTTC 
CTACAAGAGT TGGATGAATG CAAATTTACA AATCATGGCT GTCAGCACGA ATGCATCAAT 
ACTTTGGGGA GCTATCAGTG TGCTTGCTTT GCTGGCTATG AGCTGCAAGC CGACGGTCGC 
ACTTGCGAAG ATGCGTGCGG CGGCATTATC AATGCCTCTA TTCCCAATGG GACACTTCAT 
TCGCCGTCAT TTCCGGATGT GTACCCCATA TCGAAGGAGT GTGTTTGGGA GGTGGTCGCA 
CCGCCCAATC ATGCGGTCTT CCTTAATTTT ACGCACTTCG ATTTGGAAGG CACAAAGTAT 
CAATACACAG AATGTAATTA CGATTACCTA TTGGCGTACT CTAAGTTACG TGATAGTCGT 
CTAAAGAAAA TCGGTATTTA TTGTGGTCAA GAGCTACCAC CGGTATTGAG GTCCGAACAT 
AATGTACTGC GCTTGGAATT CTATTCAGAC AAAAATATTC AACGGACAGG CTTTTCAGCC 
AATATTGTAA TTGATTTCGA CGAATGCAGC GTGGACAATG GAGGCTGCCA ACATCATTGC 
CTCAATACAT TCGGATCATA TAAGTGCAGT TGCCGCAATG CATTCACTCT CCACGAGAAT 
CAGCACAATT GCACTGAGAC CAAATGCAAG TTTGAGATCA CTAGCCCCAA AGGAACCATT 
TACAGCCCCA ACTTCCCAAA TGAATATCCA CACAACACAT ACTGTTTTTG GCATTTCCGC 
ACCGTTCTGG GCCACCGAGT GCAGTTGACA TTTCATGAAT TCGAAATGGA AAACCATCAG 
GAATGTTTTT ACGATTATCT CGCCTTATAT GATGGCAAAT CTGAGAACAG CTCAACATTG 
GGCATTTACT GTGGAGGACA TGAGCCGTAT GCGGTAGTAG CATCCACAAA TGAAATGTTC 
ATGGTCCTGC GGACAGATGC ATCGCTGCAG CGTAAAGGAT TCCGAGCAAC ATACTCAACA 
GAATGCGGCG GCTACTTGCA AGCAACAAAT GAAACGCAAG TCTTTTATTC ACATCCACGC 
TATGGCAGTC GGGTATACAA TCGGCACATG TATTGTAATT GGCGTATACA AGCTGATTCT 
GAGAGTAGTG TTCAAATAAA ATTTTTGCAC TTTGAGGTCG AATATTCGGA TCGCTGTAAA 
TATGATGCGG TGGAAATAAG AGAAGAAATA TTTGAAGAGA AGTATATGGA CGTAAGAAGC 
AATCATGGAC GTTATTGTGG CAATAGAAAG CCACCTACAA TAACATCATA TACCGATACG 
TTACTGATGA GATTTCAAAC GGATGGCAGT ACCTCGTTGC GTGGTTTTGC TGTTTCTTTC 
GTAGCCGTAG AGCCGCCGGA AGAGGATCTT ATAGACGAGC TAGACGCTGT TACACCTTTT 
CCTGGTTACA TGCGAAGTGT GTACTATGCA TCGGATACAG GCAGCGATTT CGATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011186017.1
CDS	216..3275
Translation	MKGDDTSGIPRQRLQSLAFRRRLFKQLKGKRVPVHKKKHHKRRIFQSPNEAKSRHNKVKSSVNHNPNLDSFNHFRNTPYLSSVPRSRRAVTAKKERIWDYGVIPYTIDEIFSGVHKALFMRAMRHWENSTCIKFVERDAKVHPNYIHFTVKNCGCCSFVGKRGSGRQAISIGRNCEKFGIVVHELGHVIGFWHEHTRTDRDKHISINKENIMKGQEYNFDVLSPEDVDSLGLPYDYNSIMHYAKNTFAKNVYLETIQPIGLSKSQHIEIGQRLRLSTGDIIKANRLYKCSSCGRTFQENSGEIISPHYEYSAYSAIQDSNVIEDSGSGDYEVSDFDKSLEKCEWRITATNGERIILQIHQVHLLQSTNCAVDYLEIRDGYWYKSPVIKRLCGNVTEETVRSSSSRMLISYVNHHAVRGYRGFSANFEVTCGGDIFLTESRRVDSPNYPLEYLPDRECIWRISAPENRQVGLKFQSFELENHDNCAYDYVEIRDGNDSDSQLIGIYCGYILPPNIKSNSNQMFVKFVSDGSVQKVGFSAVFLQELDECKFTNHGCQHECINTLGSYQCACFAGYELQADGRTCEDACGGIINASIPNGTLHSPSFPDVYPISKECVWEVVAPPNHAVFLNFTHFDLEGTKYQYTECNYDYLLAYSKLRDSRLKKIGIYCGQELPPVLRSEHNVLRLEFYSDKNIQRTGFSANIVIDFDECSVDNGGCQHHCLNTFGSYKCSCRNAFTLHENQHNCTETKCKFEITSPKGTIYSPNFPNEYPHNTYCFWHFRTVLGHRVQLTFHEFEMENHQECFYDYLALYDGKSENSSTLGIYCGGHEPYAVVASTNEMFMVLRTDASLQRKGFRATYSTECGGYLQATNETQVFYSHPRYGSRVYNRHMYCNWRIQADSESSVQIKFLHFEVEYSDRCKYDAVEIREEIFEEKYMDVRSNHGRYCGNRKPPTITSYTDTLLMRFQTDGSTSLRGFAVSFVAVEPPEEDLIDELDAVTPFPGYMRSVYYASDTGSDFDD

Target ORF information:

RefSeq Version	XM_011187715.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae dorsal-ventral patterning protein tolloid (LOC105214345), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011187715.1

ORF Insert Sequence:

ATGAAGGGAG ACGACACAAG CGGCATCCCA CGCCAGCGGC TACAGAGCTT GGCATTTCGA 
AGGCGTTTAT TTAAACAACT TAAAGGCAAA AGGGTACCGG TCCACAAAAA GAAGCACCAC 
AAGAGACGTA TTTTTCAGTC TCCCAATGAA GCGAAAAGCA GGCACAACAA GGTCAAATCA 
AGTGTCAATC ATAACCCTAA TCTAGACTCA TTCAACCACT TTAGAAACAC TCCATATTTA 
AGTTCAGTAC CTCGTTCACG GCGTGCAGTT ACCGCCAAAA AGGAACGCAT TTGGGATTAC 
GGTGTCATAC CGTATACCAT CGACGAGATA TTCAGCGGGG TACACAAGGC GCTCTTCATG 
AGAGCCATGC GCCATTGGGA GAATTCCACA TGTATTAAAT TTGTTGAACG CGATGCTAAG 
GTGCACCCTA ATTACATACA TTTTACTGTT AAAAATTGTG GTTGCTGCTC GTTTGTGGGC 
AAGCGCGGTT CCGGACGACA AGCCATTTCG ATTGGTCGCA ACTGTGAAAA ATTCGGAATT 
GTCGTGCATG AATTGGGACA TGTTATTGGG TTTTGGCACG AACATACGCG CACCGACCGA 
GATAAGCATA TATCTATAAA TAAGGAGAAT ATCATGAAGG GGCAGGAGTA TAATTTCGAT 
GTGCTTTCAC CGGAGGATGT TGACTCACTG GGTCTACCAT ATGATTATAA TTCAATCATG 
CATTACGCAA AGAATACTTT TGCCAAGAAT GTTTATTTGG AGACCATTCA GCCTATTGGA 
CTATCGAAGT CCCAACATAT AGAGATCGGC CAAAGATTAC GTCTAAGTAC GGGGGATATA 
ATTAAGGCGA ATCGATTATA TAAATGCAGC AGTTGCGGAC GTACATTCCA AGAGAATTCT 
GGAGAGATAA TCTCACCTCA TTACGAATAC TCAGCATATT CGGCGATACA AGACAGTAAT 
GTGATAGAAG ACAGCGGAAG CGGTGATTAC GAGGTCAGTG ATTTTGACAA ATCTCTAGAG 
AAATGCGAAT GGCGTATCAC AGCAACAAAT GGGGAACGTA TTATTTTGCA AATTCATCAA 
GTGCATCTTC TGCAGTCCAC TAACTGTGCT GTGGATTATT TAGAAATACG AGACGGTTAT 
TGGTACAAAT CTCCTGTTAT TAAGAGACTA TGCGGCAATG TCACGGAGGA AACGGTTAGG 
AGCAGCTCCA GTCGAATGCT CATCAGTTAT GTTAATCACC ACGCAGTCAG AGGATACCGT 
GGATTTTCGG CCAATTTCGA GGTTACATGT GGTGGTGACA TTTTTCTCAC CGAAAGTAGA 
CGTGTTGACT CACCCAATTA TCCATTGGAA TATCTGCCGG ATCGTGAATG CATTTGGCGC 
ATATCGGCAC CGGAGAACCG TCAAGTTGGG CTGAAATTTC AGTCATTCGA ATTGGAGAAT 
CATGATAACT GTGCTTACGA CTATGTGGAA ATACGCGATG GTAATGACAG TGACTCTCAA 
TTAATTGGAA TTTACTGTGG ATATATTCTA CCACCAAATA TCAAGTCTAA TTCAAATCAA 
ATGTTTGTAA AGTTTGTTTC CGATGGTTCG GTCCAGAAGG TTGGCTTTTC CGCGGTCTTC 
CTACAAGAGT TGGATGAATG CAAATTTACA AATCATGGCT GTCAGCACGA ATGCATCAAT 
ACTTTGGGGA GCTATCAGTG TGCTTGCTTT GCTGGCTATG AGCTGCAAGC CGACGGTCGC 
ACTTGCGAAG ATGCGTGCGG CGGCATTATC AATGCCTCTA TTCCCAATGG GACACTTCAT 
TCGCCGTCAT TTCCGGATGT GTACCCCATA TCGAAGGAGT GTGTTTGGGA GGTGGTCGCA 
CCGCCCAATC ATGCGGTCTT CCTTAATTTT ACGCACTTCG ATTTGGAAGG CACAAAGTAT 
CAATACACAG AATGTAATTA CGATTACCTA TTGGCGTACT CTAAGTTACG TGATAGTCGT 
CTAAAGAAAA TCGGTATTTA TTGTGGTCAA GAGCTACCAC CGGTATTGAG GTCCGAACAT 
AATGTACTGC GCTTGGAATT CTATTCAGAC AAAAATATTC AACGGACAGG CTTTTCAGCC 
AATATTGTAA TTGATTTCGA CGAATGCAGC GTGGACAATG GAGGCTGCCA ACATCATTGC 
CTCAATACAT TCGGATCATA TAAGTGCAGT TGCCGCAATG CATTCACTCT CCACGAGAAT 
CAGCACAATT GCACTGAGAC CAAATGCAAG TTTGAGATCA CTAGCCCCAA AGGAACCATT 
TACAGCCCCA ACTTCCCAAA TGAATATCCA CACAACACAT ACTGTTTTTG GCATTTCCGC 
ACCGTTCTGG GCCACCGAGT GCAGTTGACA TTTCATGAAT TCGAAATGGA AAACCATCAG 
GAATGTTTTT ACGATTATCT CGCCTTATAT GATGGCAAAT CTGAGAACAG CTCAACATTG 
GGCATTTACT GTGGAGGACA TGAGCCGTAT GCGGTAGTAG CATCCACAAA TGAAATGTTC 
ATGGTCCTGC GGACAGATGC ATCGCTGCAG CGTAAAGGAT TCCGAGCAAC ATACTCAACA 
GAATGCGGCG GCTACTTGCA AGCAACAAAT GAAACGCAAG TCTTTTATTC ACATCCACGC 
TATGGCAGTC GGGTATACAA TCGGCACATG TATTGTAATT GGCGTATACA AGCTGATTCT 
GAGAGTAGTG TTCAAATAAA ATTTTTGCAC TTTGAGGTCG AATATTCGGA TCGCTGTAAA 
TATGATGCGG TGGAAATAAG AGAAGAAATA TTTGAAGAGA AGTATATGGA CGTAAGAAGC 
AATCATGGAC GTTATTGTGG CAATAGAAAG CCACCTACAA TAACATCATA TACCGATACG 
TTACTGATGA GATTTCAAAC GGATGGCAGT ACCTCGTTGC GTGGTTTTGC TGTTTCTTTC 
GTAGCCGTAG AGCCGCCGGA AGAGGATCTT ATAGACGAGC TAGACGCTGT TACACCTTTT 
CCTGGTTACA TGCGAAGTGT GTACTATGCA TCGGATACAG GCAGCGATTT CGATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105214345 ( XM_011187715.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011186017.2 Zeugodacus cucurbitae dorsal-ventral patterning protein tolloid (LOC105214345), mRNA.

Price & Availability↑

CloneID	OBa256417
Clone ID Related Accession (Same CDS sequence)	XM_011187715.2
Accession Version	XM_011187715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	dorsal-ventral patterning protein tolloid
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863728.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011187715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGTTTTGT TGCATGGACG ACAGTATCTG TTATTGTTGT TTTTGTTACC GCTAACGGCA 
GTGTCTTGTA GCGCCAAGAA TACACATTCA AATCAGCGCA AACACAACCA TCCTCGGTAT 
TTGAGTCGTC AACAAAAGAG TGCACTTGCC CGCCAATTGC TCCTCGTCGA TGGGTTGCAG 
GTGGAATCGG AGAGTTATTT CGACGTAGTG GAACAACCAG AACAATTTGT GGAAATCGAT 
ACGCAAATCG CGCCAGAAAC GAAAGGCAAA CATGGCGCCA TTGAACCAAC TCATCTAAAG 
CATTTGCTTG ATATCGCCGC CGTTGAGTCG GAGATGTCAG CCGCCATTGC AGCCGGCGAC 
GAGGAGGCGA AGAACGTCTG GAGTCGCGCC AACGCAAATC GGCCGGCCTT CCTTTTCGAC 
AGCGGCGCTT TGTCGAAAAT ACAATCGAAT AATACAAAAA GTATAAACAC CGATGATGGA 
GGGAATTTTT TCTACGAACG AAACATTATT ACCGAAGAGC CGGGAGTATC GCTAGCAAAG 
CTTAAAGCAG ACCGCCAACA AAAGAACACA ATGAAGGGAG ACGACACAAG CGGCATCCCA 
CGCCAGCGGC TACAGAGCTT GGCATTTCGA AGGCGTTTAT TTAAACAACT TAAAGGCAAA 
AGGGTACCGG TCCACAAAAA GAAGCACCAC AAGAGACGTA TTTTTCAGTC TCCCAATGAA 
GCGAAAAGCA GGCACAACAA GGTCAAATCA AGTGTCAATC ATAACCCTAA TCTAGACTCA 
TTCAACCACT TTAGAAACAC TCCATATTTA AGTTCAGTAC CTCGTTCACG GCGTGCAGTT 
ACCGCCAAAA AGGAACGCAT TTGGGATTAC GGTGTCATAC CGTATACCAT CGACGAGATA 
TTCAGCGGGG TACACAAGGC GCTCTTCATG AGAGCCATGC GCCATTGGGA GAATTCCACA 
TGTATTAAAT TTGTTGAACG CGATGCTAAG GTGCACCCTA ATTACATACA TTTTACTGTT 
AAAAATTGTG GTTGCTGCTC GTTTGTGGGC AAGCGCGGTT CCGGACGACA AGCCATTTCG 
ATTGGTCGCA ACTGTGAAAA ATTCGGAATT GTCGTGCATG AATTGGGACA TGTTATTGGG 
TTTTGGCACG AACATACGCG CACCGACCGA GATAAGCATA TATCTATAAA TAAGGAGAAT 
ATCATGAAGG GGCAGGAGTA TAATTTCGAT GTGCTTTCAC CGGAGGATGT TGACTCACTG 
GGTCTACCAT ATGATTATAA TTCAATCATG CATTACGCAA AGAATACTTT TGCCAAGAAT 
GTTTATTTGG AGACCATTCA GCCTATTGGA CTATCGAAGT CCCAACATAT AGAGATCGGC 
CAAAGATTAC GTCTAAGTAC GGGGGATATA ATTAAGGCGA ATCGATTATA TAAATGCAGC 
AGTTGCGGAC GTACATTCCA AGAGAATTCT GGAGAGATAA TCTCACCTCA TTACGAATAC 
TCAGCATATT CGGCGATACA AGACAGTAAT GTGATAGAAG ACAGCGGAAG CGGTGATTAC 
GAGGTCAGTG ATTTTGACAA ATCTCTAGAG AAATGCGAAT GGCGTATCAC AGCAACAAAT 
GGGGAACGTA TTATTTTGCA AATTCATCAA GTGCATCTTC TGCAGTCCAC TAACTGTGCT 
GTGGATTATT TAGAAATACG AGACGGTTAT TGGTACAAAT CTCCTGTTAT TAAGAGACTA 
TGCGGCAATG TCACGGAGGA AACGGTTAGG AGCAGCTCCA GTCGAATGCT CATCAGTTAT 
GTTAATCACC ACGCAGTCAG AGGATACCGT GGATTTTCGG CCAATTTCGA GGTTACATGT 
GGTGGTGACA TTTTTCTCAC CGAAAGTAGA CGTGTTGACT CACCCAATTA TCCATTGGAA 
TATCTGCCGG ATCGTGAATG CATTTGGCGC ATATCGGCAC CGGAGAACCG TCAAGTTGGG 
CTGAAATTTC AGTCATTCGA ATTGGAGAAT CATGATAACT GTGCTTACGA CTATGTGGAA 
ATACGCGATG GTAATGACAG TGACTCTCAA TTAATTGGAA TTTACTGTGG ATATATTCTA 
CCACCAAATA TCAAGTCTAA TTCAAATCAA ATGTTTGTAA AGTTTGTTTC CGATGGTTCG 
GTCCAGAAGG TTGGCTTTTC CGCGGTCTTC CTACAAGAGT TGGATGAATG CAAATTTACA 
AATCATGGCT GTCAGCACGA ATGCATCAAT ACTTTGGGGA GCTATCAGTG TGCTTGCTTT 
GCTGGCTATG AGCTGCAAGC CGACGGTCGC ACTTGCGAAG ATGCGTGCGG CGGCATTATC 
AATGCCTCTA TTCCCAATGG GACACTTCAT TCGCCGTCAT TTCCGGATGT GTACCCCATA 
TCGAAGGAGT GTGTTTGGGA GGTGGTCGCA CCGCCCAATC ATGCGGTCTT CCTTAATTTT 
ACGCACTTCG ATTTGGAAGG CACAAAGTAT CAATACACAG AATGTAATTA CGATTACCTA 
TTGGCGTACT CTAAGTTACG TGATAGTCGT CTAAAGAAAA TCGGTATTTA TTGTGGTCAA 
GAGCTACCAC CGGTATTGAG GTCCGAACAT AATGTACTGC GCTTGGAATT CTATTCAGAC 
AAAAATATTC AACGGACAGG CTTTTCAGCC AATATTGTAA TTGATTTCGA CGAATGCAGC 
GTGGACAATG GAGGCTGCCA ACATCATTGC CTCAATACAT TCGGATCATA TAAGTGCAGT 
TGCCGCAATG CATTCACTCT CCACGAGAAT CAGCACAATT GCACTGAGAC CAAATGCAAG 
TTTGAGATCA CTAGCCCCAA AGGAACCATT TACAGCCCCA ACTTCCCAAA TGAATATCCA 
CACAACACAT ACTGTTTTTG GCATTTCCGC ACCGTTCTGG GCCACCGAGT GCAGTTGACA 
TTTCATGAAT TCGAAATGGA AAACCATCAG GAATGTTTTT ACGATTATCT CGCCTTATAT 
GATGGCAAAT CTGAGAACAG CTCAACATTG GGCATTTACT GTGGAGGACA TGAGCCGTAT 
GCGGTAGTAG CATCCACAAA TGAAATGTTC ATGGTCCTGC GGACAGATGC ATCGCTGCAG 
CGTAAAGGAT TCCGAGCAAC ATACTCAACA GAATGCGGCG GCTACTTGCA AGCAACAAAT 
GAAACGCAAG TCTTTTATTC ACATCCACGC TATGGCAGTC GGGTATACAA TCGGCACATG 
TATTGTAATT GGCGTATACA AGCTGATTCT GAGAGTAGTG TTCAAATAAA ATTTTTGCAC 
TTTGAGGTCG AATATTCGGA TCGCTGTAAA TATGATGCGG TGGAAATAAG AGAAGAAATA 
TTTGAAGAGA AGTATATGGA CGTAAGAAGC AATCATGGAC GTTATTGTGG CAATAGAAAG 
CCACCTACAA TAACATCATA TACCGATACG TTACTGATGA GATTTCAAAC GGATGGCAGT 
ACCTCGTTGC GTGGTTTTGC TGTTTCTTTC GTAGCCGTAG AGCCGCCGGA AGAGGATCTT 
ATAGACGAGC TAGACGCTGT TACACCTTTT CCTGGTTACA TGCGAAGTGT GTACTATGCA 
TCGGATACAG GCAGCGATTT CGATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011186017.2
CDS	1..3630
Translation	MVLLHGRQYLLLLFLLPLTAVSCSAKNTHSNQRKHNHPRYLSRQQKSALARQLLLVDGLQVESESYFDVVEQPEQFVEIDTQIAPETKGKHGAIEPTHLKHLLDIAAVESEMSAAIAAGDEEAKNVWSRANANRPAFLFDSGALSKIQSNNTKSINTDDGGNFFYERNIITEEPGVSLAKLKADRQQKNTMKGDDTSGIPRQRLQSLAFRRRLFKQLKGKRVPVHKKKHHKRRIFQSPNEAKSRHNKVKSSVNHNPNLDSFNHFRNTPYLSSVPRSRRAVTAKKERIWDYGVIPYTIDEIFSGVHKALFMRAMRHWENSTCIKFVERDAKVHPNYIHFTVKNCGCCSFVGKRGSGRQAISIGRNCEKFGIVVHELGHVIGFWHEHTRTDRDKHISINKENIMKGQEYNFDVLSPEDVDSLGLPYDYNSIMHYAKNTFAKNVYLETIQPIGLSKSQHIEIGQRLRLSTGDIIKANRLYKCSSCGRTFQENSGEIISPHYEYSAYSAIQDSNVIEDSGSGDYEVSDFDKSLEKCEWRITATNGERIILQIHQVHLLQSTNCAVDYLEIRDGYWYKSPVIKRLCGNVTEETVRSSSSRMLISYVNHHAVRGYRGFSANFEVTCGGDIFLTESRRVDSPNYPLEYLPDRECIWRISAPENRQVGLKFQSFELENHDNCAYDYVEIRDGNDSDSQLIGIYCGYILPPNIKSNSNQMFVKFVSDGSVQKVGFSAVFLQELDECKFTNHGCQHECINTLGSYQCACFAGYELQADGRTCEDACGGIINASIPNGTLHSPSFPDVYPISKECVWEVVAPPNHAVFLNFTHFDLEGTKYQYTECNYDYLLAYSKLRDSRLKKIGIYCGQELPPVLRSEHNVLRLEFYSDKNIQRTGFSANIVIDFDECSVDNGGCQHHCLNTFGSYKCSCRNAFTLHENQHNCTETKCKFEITSPKGTIYSPNFPNEYPHNTYCFWHFRTVLGHRVQLTFHEFEMENHQECFYDYLALYDGKSENSSTLGIYCGGHEPYAVVASTNEMFMVLRTDASLQRKGFRATYSTECGGYLQATNETQVFYSHPRYGSRVYNRHMYCNWRIQADSESSVQIKFLHFEVEYSDRCKYDAVEIREEIFEEKYMDVRSNHGRYCGNRKPPTITSYTDTLLMRFQTDGSTSLRGFAVSFVAVEPPEEDLIDELDAVTPFPGYMRSVYYASDTGSDFDD

Target ORF information:

RefSeq Version	XM_011187715.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae dorsal-ventral patterning protein tolloid (LOC105214345), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011187715.2

ORF Insert Sequence:

ATGGTTTTGT TGCATGGACG ACAGTATCTG TTATTGTTGT TTTTGTTACC GCTAACGGCA 
GTGTCTTGTA GCGCCAAGAA TACACATTCA AATCAGCGCA AACACAACCA TCCTCGGTAT 
TTGAGTCGTC AACAAAAGAG TGCACTTGCC CGCCAATTGC TCCTCGTCGA TGGGTTGCAG 
GTGGAATCGG AGAGTTATTT CGACGTAGTG GAACAACCAG AACAATTTGT GGAAATCGAT 
ACGCAAATCG CGCCAGAAAC GAAAGGCAAA CATGGCGCCA TTGAACCAAC TCATCTAAAG 
CATTTGCTTG ATATCGCCGC CGTTGAGTCG GAGATGTCAG CCGCCATTGC AGCCGGCGAC 
GAGGAGGCGA AGAACGTCTG GAGTCGCGCC AACGCAAATC GGCCGGCCTT CCTTTTCGAC 
AGCGGCGCTT TGTCGAAAAT ACAATCGAAT AATACAAAAA GTATAAACAC CGATGATGGA 
GGGAATTTTT TCTACGAACG AAACATTATT ACCGAAGAGC CGGGAGTATC GCTAGCAAAG 
CTTAAAGCAG ACCGCCAACA AAAGAACACA ATGAAGGGAG ACGACACAAG CGGCATCCCA 
CGCCAGCGGC TACAGAGCTT GGCATTTCGA AGGCGTTTAT TTAAACAACT TAAAGGCAAA 
AGGGTACCGG TCCACAAAAA GAAGCACCAC AAGAGACGTA TTTTTCAGTC TCCCAATGAA 
GCGAAAAGCA GGCACAACAA GGTCAAATCA AGTGTCAATC ATAACCCTAA TCTAGACTCA 
TTCAACCACT TTAGAAACAC TCCATATTTA AGTTCAGTAC CTCGTTCACG GCGTGCAGTT 
ACCGCCAAAA AGGAACGCAT TTGGGATTAC GGTGTCATAC CGTATACCAT CGACGAGATA 
TTCAGCGGGG TACACAAGGC GCTCTTCATG AGAGCCATGC GCCATTGGGA GAATTCCACA 
TGTATTAAAT TTGTTGAACG CGATGCTAAG GTGCACCCTA ATTACATACA TTTTACTGTT 
AAAAATTGTG GTTGCTGCTC GTTTGTGGGC AAGCGCGGTT CCGGACGACA AGCCATTTCG 
ATTGGTCGCA ACTGTGAAAA ATTCGGAATT GTCGTGCATG AATTGGGACA TGTTATTGGG 
TTTTGGCACG AACATACGCG CACCGACCGA GATAAGCATA TATCTATAAA TAAGGAGAAT 
ATCATGAAGG GGCAGGAGTA TAATTTCGAT GTGCTTTCAC CGGAGGATGT TGACTCACTG 
GGTCTACCAT ATGATTATAA TTCAATCATG CATTACGCAA AGAATACTTT TGCCAAGAAT 
GTTTATTTGG AGACCATTCA GCCTATTGGA CTATCGAAGT CCCAACATAT AGAGATCGGC 
CAAAGATTAC GTCTAAGTAC GGGGGATATA ATTAAGGCGA ATCGATTATA TAAATGCAGC 
AGTTGCGGAC GTACATTCCA AGAGAATTCT GGAGAGATAA TCTCACCTCA TTACGAATAC 
TCAGCATATT CGGCGATACA AGACAGTAAT GTGATAGAAG ACAGCGGAAG CGGTGATTAC 
GAGGTCAGTG ATTTTGACAA ATCTCTAGAG AAATGCGAAT GGCGTATCAC AGCAACAAAT 
GGGGAACGTA TTATTTTGCA AATTCATCAA GTGCATCTTC TGCAGTCCAC TAACTGTGCT 
GTGGATTATT TAGAAATACG AGACGGTTAT TGGTACAAAT CTCCTGTTAT TAAGAGACTA 
TGCGGCAATG TCACGGAGGA AACGGTTAGG AGCAGCTCCA GTCGAATGCT CATCAGTTAT 
GTTAATCACC ACGCAGTCAG AGGATACCGT GGATTTTCGG CCAATTTCGA GGTTACATGT 
GGTGGTGACA TTTTTCTCAC CGAAAGTAGA CGTGTTGACT CACCCAATTA TCCATTGGAA 
TATCTGCCGG ATCGTGAATG CATTTGGCGC ATATCGGCAC CGGAGAACCG TCAAGTTGGG 
CTGAAATTTC AGTCATTCGA ATTGGAGAAT CATGATAACT GTGCTTACGA CTATGTGGAA 
ATACGCGATG GTAATGACAG TGACTCTCAA TTAATTGGAA TTTACTGTGG ATATATTCTA 
CCACCAAATA TCAAGTCTAA TTCAAATCAA ATGTTTGTAA AGTTTGTTTC CGATGGTTCG 
GTCCAGAAGG TTGGCTTTTC CGCGGTCTTC CTACAAGAGT TGGATGAATG CAAATTTACA 
AATCATGGCT GTCAGCACGA ATGCATCAAT ACTTTGGGGA GCTATCAGTG TGCTTGCTTT 
GCTGGCTATG AGCTGCAAGC CGACGGTCGC ACTTGCGAAG ATGCGTGCGG CGGCATTATC 
AATGCCTCTA TTCCCAATGG GACACTTCAT TCGCCGTCAT TTCCGGATGT GTACCCCATA 
TCGAAGGAGT GTGTTTGGGA GGTGGTCGCA CCGCCCAATC ATGCGGTCTT CCTTAATTTT 
ACGCACTTCG ATTTGGAAGG CACAAAGTAT CAATACACAG AATGTAATTA CGATTACCTA 
TTGGCGTACT CTAAGTTACG TGATAGTCGT CTAAAGAAAA TCGGTATTTA TTGTGGTCAA 
GAGCTACCAC CGGTATTGAG GTCCGAACAT AATGTACTGC GCTTGGAATT CTATTCAGAC 
AAAAATATTC AACGGACAGG CTTTTCAGCC AATATTGTAA TTGATTTCGA CGAATGCAGC 
GTGGACAATG GAGGCTGCCA ACATCATTGC CTCAATACAT TCGGATCATA TAAGTGCAGT 
TGCCGCAATG CATTCACTCT CCACGAGAAT CAGCACAATT GCACTGAGAC CAAATGCAAG 
TTTGAGATCA CTAGCCCCAA AGGAACCATT TACAGCCCCA ACTTCCCAAA TGAATATCCA 
CACAACACAT ACTGTTTTTG GCATTTCCGC ACCGTTCTGG GCCACCGAGT GCAGTTGACA 
TTTCATGAAT TCGAAATGGA AAACCATCAG GAATGTTTTT ACGATTATCT CGCCTTATAT 
GATGGCAAAT CTGAGAACAG CTCAACATTG GGCATTTACT GTGGAGGACA TGAGCCGTAT 
GCGGTAGTAG CATCCACAAA TGAAATGTTC ATGGTCCTGC GGACAGATGC ATCGCTGCAG 
CGTAAAGGAT TCCGAGCAAC ATACTCAACA GAATGCGGCG GCTACTTGCA AGCAACAAAT 
GAAACGCAAG TCTTTTATTC ACATCCACGC TATGGCAGTC GGGTATACAA TCGGCACATG 
TATTGTAATT GGCGTATACA AGCTGATTCT GAGAGTAGTG TTCAAATAAA ATTTTTGCAC 
TTTGAGGTCG AATATTCGGA TCGCTGTAAA TATGATGCGG TGGAAATAAG AGAAGAAATA 
TTTGAAGAGA AGTATATGGA CGTAAGAAGC AATCATGGAC GTTATTGTGG CAATAGAAAG 
CCACCTACAA TAACATCATA TACCGATACG TTACTGATGA GATTTCAAAC GGATGGCAGT 
ACCTCGTTGC GTGGTTTTGC TGTTTCTTTC GTAGCCGTAG AGCCGCCGGA AGAGGATCTT 
ATAGACGAGC TAGACGCTGT TACACCTTTT CCTGGTTACA TGCGAAGTGT GTACTATGCA 
TCGGATACAG GCAGCGATTT CGATGATTAA