micall1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	micall1
Entrez Gene ID	779550
Full Name	MICAL like 1
Synonyms	mical-l1,mirab13
General protein information	Preferred Names MICAL like 1 Names MICAL-like protein 1 novel LIM domain containing protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031901085.1	XP_031756945.1	MICAL-like protein 1
XM_002934234.4	XP_002934280.2	MICAL-like protein 1 isoform X1
XM_018093657.1	XP_017949146.1	MICAL-like protein 1 isoform X2
XM_002934234.5	XP_002934280.2	MICAL-like protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MICALL1	XP_515124.3
Canis lupus familiaris (dog)	MICALL1	XP_005625951.1
Bos taurus (cattle)	MICALL1	NP_001179995.1
Danio rerio (zebrafish)	micall1	XP_001922175.2
Xenopus tropicalis (tropical clawed frog)	micall1	XP_004913938.1
Homo sapiens (human)	MICALL1	NP_203744.1
Rattus norvegicus (Norway rat)	Micall1	XP_002729878.2
Mus musculus (house mouse)	Micall1	NP_803412.1
Gallus gallus (chicken)	MICALL1	NP_001188377.1

Gene Ontology

The following micall1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the micall1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa09312	XM_031901085.1 Latest version!	Xenopus tropicalis MICAL like 1 (micall1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa09312	XM_002934234.5 Latest version!	Xenopus tropicalis MICAL like 1 (micall1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa44227	XM_018093657.1 Latest version!	Xenopus tropicalis MICAL like 1 (micall1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OXa09312	XM_002934234.4	Xenopus tropicalis MICAL like 1 (micall1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

micall1 ( XM_002934234.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934280.2 Xenopus tropicalis MICAL like 1 (micall1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa09312
Clone ID Related Accession (Same CDS sequence)	XM_031901085.1 , XM_002934234.4 , XM_002934234.5
Accession Version	XM_002934234.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MICAL-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934234.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCT CACGGAATCT ACAGCAGTGG TGCTCCCGGC AGTGTGAGGG TTACCCTGGC 
ATTCTGATCT ATGACCTCAG CTCATCTTTT CGGGATGGAC TGGCCTTCTG CGCTCTCATT 
CACCGCCATC GGCCTGATCT CATAGACTTT GATTCTCTTT CTAAAGAAAA TGTCTTCGAG 
AACAACCGCC TGGCTTTTGA AGTGGCTGAA CGGGAACTGG GTATTCCAGC TTTGCTTGAT 
CCAGAGGACA TGGTGTCCAT GAAAGTGCCT GATCGACTGA GCATCATGAC GTACTTATCC 
CAGTATTATA ACCACTTCCG GAATTCTAGT CCAGTGGGAA AGCCTCAGCT GAAAGAAACC 
TCTGAGTCCC CCAGCAAAGA GCCCAGTGGA GGTTCCTTAC CTCAGGGAAG CCCAAATGCC 
CAGACCTCTT TGATCCGTAG CTCAATAAGC AGCACCTGTG CTCTGTGCCA GCAGCATGTA 
CACCTAGTGC AGAGATACCT GGTAGAGGGG AAGCTTTATC ACCGCCAGTG CTTCAGATGT 
AAAGAATGCT CAAGTACCCT AGTTCCAGGA GCCTACCGCC CAGGCACTGA TGCCGGCACT 
CTGGTTTGCA CTCACCACTG CCACCGGCCA CCAACCAGCA GCCCTGAATC TGAAGAAGAT 
GGCACTAAGC AAAAAGCAGT TCAGACCCCT CCCAAACCAT TTGTGCCAGA AAAACCTGCC 
TCCTCCAATA AACCTCCAAC AGAATTGCAT CCTGATCCCT TTCCTAACAT GCGACCAGTA 
CCAGCTCCAA GAAGAACAAG TGATGTGGCC CCTCAGCCTC TGCCACGCTC CCGTGCAGCA 
GCAGAATCAA GCCCAGTGAG GCCTACATCT CTAATAAATG GCGAACAACA GTCTCCCGCT 
CCACCAATAC CCAAACCAAG AAGCAGACAG AGATCCTCAG AAAGGGAGGA TGAAGAAAAA 
CAGAAAAAAA ATCCTCCTTG GCTCTCTCTG GTTCCTGCGA CAGAGCCCAA AAGGAGAGCT 
GCTCCCCCAC CTCCACCACT GGCTCAAAAG CCGGAGTTAG GAGATGAGAA ATCTCAGAAG 
TCTTTAAATG ACACAGAAAA GCAAAGTCTC TCATCAAAGC CAACAGCCTA CAATCCATTT 
GAAGATGAAG AAGAGGAAGC GGAAACAAGT TCTCTAAAAC CAGGCCACCC ATGGTATGGA 
ATTACACCAA CCAGCAGCCC CAAGAGTCGC AAAAGGCTAG CACCTAAAGC TCCCAATGCT 
TCACCTCTGG CTCATCATTC TAACCTAGTG TCAAGACTGT CTCACTCGGA GCCTCCCTCG 
GGCAGCCCTT CCCCAGCCCT AAGTACAGAG AGCCTTCCAA CGGATGCTTC CACAAAGGGC 
CAGGATCCTG AGAGTGTTCC CAAAAGCTCA TCTGAACCAA CTATCCATTC TCCAAACAAG 
CCAGAACCTT CAGATAGAGA TACTACTACC TACCTCAGCA CTGGTGTCCC CAGTACTTCT 
ACCAACACCA GCCTTTCTTC CTCTAGCGAA TTAGCTAATA CTAATGAAGC ACAAGAAAGC 
CCATCCCCAG AAATGCCTCT TAGTCCAAGC CGACCTGCCC CTCAAGTACC TACCATCTCC 
ATTCCTAGCA CAGTGGCATC AGACAGCACA CCTCCAGAGA AAAAGCCTCT AGCAAAGCAA 
ATTTGCAAAC AGAATCCCTT TAATAGGAAA TCCTCTCCTG CTACATCTCC CATCAACAAA 
AGGAACCAGA AAGGTCCAAA ACCTTCCCGA CCCCCTGCTC CAGGACATGG GTTCCCCCTT 
ATAAAACGCA AGGTTCAGGC TGATGACTAC ATTCCGGAAG ATGAGATTCA GATGGAGATG 
GAGACAATTG AGCTGCGCCT GGATGAGCTA GAAAGGCGTG GGGTGGAGCT GGAACACAGA 
CTGCGCAAGT TGGAGAATGA GTCAGATGAA GATAGTCTGC TGGTGGACTG GTTCAAGCTG 
ATCCAAGAAA AACACACCCT GGTGCGCCGG GAGTCAGAGC TTGTGTACAC CACTAAACAA 
CAGAGCCTGG AACAGCGTCA GGCTGATGTG GAGTATGAAC TTCGCTGCTT ACTGAATAAG 
CCAGAAAAGG ACTGGCTGGA AGAGGATAAG GAACGGGAAC AAGTGCTAAT GCAGGAGCTG 
GTCACACTAA TTGAGCAGAG AAATGCTATT GTCAAGTGCT TGGATGAAGA CAGACAGAGG 
GAAAAAGAAG AGGACAAGAT CATTGAAGCT ATGATACTGA AGAAAGATTT TCATACAGAT 
CCGCAAAAAA AAAAGAAAGG AAAATTTAAA CCTATAAAAG TTCTTAAGCT TCTTTCTCCA 
AAGCAAGAGC CCAAAGTAAA AAGCCCTAAG GACAAAAACA AAGTGCAATC GGATGGGACC 
AAGAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934280.2
CDS	217..2625
Translation	MSASRNLQQWCSRQCEGYPGILIYDLSSSFRDGLAFCALIHRHRPDLIDFDSLSKENVFENNRLAFEVAERELGIPALLDPEDMVSMKVPDRLSIMTYLSQYYNHFRNSSPVGKPQLKETSESPSKEPSGGSLPQGSPNAQTSLIRSSISSTCALCQQHVHLVQRYLVEGKLYHRQCFRCKECSSTLVPGAYRPGTDAGTLVCTHHCHRPPTSSPESEEDGTKQKAVQTPPKPFVPEKPASSNKPPTELHPDPFPNMRPVPAPRRTSDVAPQPLPRSRAAAESSPVRPTSLINGEQQSPAPPIPKPRSRQRSSEREDEEKQKKNPPWLSLVPATEPKRRAAPPPPPLAQKPELGDEKSQKSLNDTEKQSLSSKPTAYNPFEDEEEEAETSSLKPGHPWYGITPTSSPKSRKRLAPKAPNASPLAHHSNLVSRLSHSEPPSGSPSPALSTESLPTDASTKGQDPESVPKSSSEPTIHSPNKPEPSDRDTTTYLSTGVPSTSTNTSLSSSSELANTNEAQESPSPEMPLSPSRPAPQVPTISIPSTVASDSTPPEKKPLAKQICKQNPFNRKSSPATSPINKRNQKGPKPSRPPAPGHGFPLIKRKVQADDYIPEDEIQMEMETIELRLDELERRGVELEHRLRKLENESDEDSLLVDWFKLIQEKHTLVRRESELVYTTKQQSLEQRQADVEYELRCLLNKPEKDWLEEDKEREQVLMQELVTLIEQRNAIVKCLDEDRQREKEEDKIIEAMILKKDFHTDPQKKKKGKFKPIKVLKLLSPKQEPKVKSPKDKNKVQSDGTKR

Target ORF information:

RefSeq Version	XM_002934234.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MICAL like 1 (micall1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934234.4

ORF Insert Sequence:

ATGTCGGCCT CACGGAATCT ACAGCAGTGG TGCTCCCGGC AGTGTGAGGG TTACCCTGGC 
ATTCTGATCT ATGACCTCAG CTCATCTTTT CGGGATGGAC TGGCCTTCTG CGCTCTCATT 
CACCGCCATC GGCCTGATCT CATAGACTTT GATTCTCTTT CTAAAGAAAA TGTCTTCGAG 
AACAACCGCC TGGCTTTTGA AGTGGCTGAA CGGGAACTGG GTATTCCAGC TTTGCTTGAT 
CCAGAGGACA TGGTGTCCAT GAAAGTGCCT GATCGACTGA GCATCATGAC GTACTTATCC 
CAGTATTATA ACCACTTCCG GAATTCTAGT CCAGTGGGAA AGCCTCAGCT GAAAGAAACC 
TCTGAGTCCC CCAGCAAAGA GCCCAGTGGA GGTTCCTTAC CTCAGGGAAG CCCAAATGCC 
CAGACCTCTT TGATCCGTAG CTCAATAAGC AGCACCTGTG CTCTGTGCCA GCAGCATGTA 
CACCTAGTGC AGAGATACCT GGTAGAGGGG AAGCTTTATC ACCGCCAGTG CTTCAGATGT 
AAAGAATGCT CAAGTACCCT AGTTCCAGGA GCCTACCGCC CAGGCACTGA TGCCGGCACT 
CTGGTTTGCA CTCACCACTG CCACCGGCCA CCAACCAGCA GCCCTGAATC TGAAGAAGAT 
GGCACTAAGC AAAAAGCAGT TCAGACCCCT CCCAAACCAT TTGTGCCAGA AAAACCTGCC 
TCCTCCAATA AACCTCCAAC AGAATTGCAT CCTGATCCCT TTCCTAACAT GCGACCAGTA 
CCAGCTCCAA GAAGAACAAG TGATGTGGCC CCTCAGCCTC TGCCACGCTC CCGTGCAGCA 
GCAGAATCAA GCCCAGTGAG GCCTACATCT CTAATAAATG GCGAACAACA GTCTCCCGCT 
CCACCAATAC CCAAACCAAG AAGCAGACAG AGATCCTCAG AAAGGGAGGA TGAAGAAAAA 
CAGAAAAAAA ATCCTCCTTG GCTCTCTCTG GTTCCTGCGA CAGAGCCCAA AAGGAGAGCT 
GCTCCCCCAC CTCCACCACT GGCTCAAAAG CCGGAGTTAG GAGATGAGAA ATCTCAGAAG 
TCTTTAAATG ACACAGAAAA GCAAAGTCTC TCATCAAAGC CAACAGCCTA CAATCCATTT 
GAAGATGAAG AAGAGGAAGC GGAAACAAGT TCTCTAAAAC CAGGCCACCC ATGGTATGGA 
ATTACACCAA CCAGCAGCCC CAAGAGTCGC AAAAGGCTAG CACCTAAAGC TCCCAATGCT 
TCACCTCTGG CTCATCATTC TAACCTAGTG TCAAGACTGT CTCACTCGGA GCCTCCCTCG 
GGCAGCCCTT CCCCAGCCCT AAGTACAGAG AGCCTTCCAA CGGATGCTTC CACAAAGGGC 
CAGGATCCTG AGAGTGTTCC CAAAAGCTCA TCTGAACCAA CTATCCATTC TCCAAACAAG 
CCAGAACCTT CAGATAGAGA TACTACTACC TACCTCAGCA CTGGTGTCCC CAGTACTTCT 
ACCAACACCA GCCTTTCTTC CTCTAGCGAA TTAGCTAATA CTAATGAAGC ACAAGAAAGC 
CCATCCCCAG AAATGCCTCT TAGTCCAAGC CGACCTGCCC CTCAAGTACC TACCATCTCC 
ATTCCTAGCA CAGTGGCATC AGACAGCACA CCTCCAGAGA AAAAGCCTCT AGCAAAGCAA 
ATTTGCAAAC AGAATCCCTT TAATAGGAAA TCCTCTCCTG CTACATCTCC CATCAACAAA 
AGGAACCAGA AAGGTCCAAA ACCTTCCCGA CCCCCTGCTC CAGGACATGG GTTCCCCCTT 
ATAAAACGCA AGGTTCAGGC TGATGACTAC ATTCCGGAAG ATGAGATTCA GATGGAGATG 
GAGACAATTG AGCTGCGCCT GGATGAGCTA GAAAGGCGTG GGGTGGAGCT GGAACACAGA 
CTGCGCAAGT TGGAGAATGA GTCAGATGAA GATAGTCTGC TGGTGGACTG GTTCAAGCTG 
ATCCAAGAAA AACACACCCT GGTGCGCCGG GAGTCAGAGC TTGTGTACAC CACTAAACAA 
CAGAGCCTGG AACAGCGTCA GGCTGATGTG GAGTATGAAC TTCGCTGCTT ACTGAATAAG 
CCAGAAAAGG ACTGGCTGGA AGAGGATAAG GAACGGGAAC AAGTGCTAAT GCAGGAGCTG 
GTCACACTAA TTGAGCAGAG AAATGCTATT GTCAAGTGCT TGGATGAAGA CAGACAGAGG 
GAAAAAGAAG AGGACAAGAT CATTGAAGCT ATGATACTGA AGAAAGATTT TCATACAGAT 
CCGCAAAAAA AAAAGAAAGG AAAATTTAAA CCTATAAAAG TTCTTAAGCT TCTTTCTCCA 
AAGCAAGAGC CCAAAGTAAA AAGCCCTAAG GACAAAAACA AAGTGCAATC GGATGGGACC 
AAGAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

micall1 ( XM_031901085.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756945.1 Xenopus tropicalis MICAL like 1 (micall1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa09312
Clone ID Related Accession (Same CDS sequence)	XM_031901085.1 , XM_002934234.4 , XM_002934234.5
Accession Version	XM_031901085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MICAL-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCT CACGGAATCT ACAGCAGTGG TGCTCCCGGC AGTGTGAGGG TTACCCTGGC 
ATTCTGATCT ATGACCTCAG CTCATCTTTT CGGGATGGAC TGGCCTTCTG CGCTCTCATT 
CACCGCCATC GGCCTGATCT CATAGACTTT GATTCTCTTT CTAAAGAAAA TGTCTTCGAG 
AACAACCGCC TGGCTTTTGA AGTGGCTGAA CGGGAACTGG GTATTCCAGC TTTGCTTGAT 
CCAGAGGACA TGGTGTCCAT GAAAGTGCCT GATCGACTGA GCATCATGAC GTACTTATCC 
CAGTATTATA ACCACTTCCG GAATTCTAGT CCAGTGGGAA AGCCTCAGCT GAAAGAAACC 
TCTGAGTCCC CCAGCAAAGA GCCCAGTGGA GGTTCCTTAC CTCAGGGAAG CCCAAATGCC 
CAGACCTCTT TGATCCGTAG CTCAATAAGC AGCACCTGTG CTCTGTGCCA GCAGCATGTA 
CACCTAGTGC AGAGATACCT GGTAGAGGGG AAGCTTTATC ACCGCCAGTG CTTCAGATGT 
AAAGAATGCT CAAGTACCCT AGTTCCAGGA GCCTACCGCC CAGGCACTGA TGCCGGCACT 
CTGGTTTGCA CTCACCACTG CCACCGGCCA CCAACCAGCA GCCCTGAATC TGAAGAAGAT 
GGCACTAAGC AAAAAGCAGT TCAGACCCCT CCCAAACCAT TTGTGCCAGA AAAACCTGCC 
TCCTCCAATA AACCTCCAAC AGAATTGCAT CCTGATCCCT TTCCTAACAT GCGACCAGTA 
CCAGCTCCAA GAAGAACAAG TGATGTGGCC CCTCAGCCTC TGCCACGCTC CCGTGCAGCA 
GCAGAATCAA GCCCAGTGAG GCCTACATCT CTAATAAATG GCGAACAACA GTCTCCCGCT 
CCACCAATAC CCAAACCAAG AAGCAGACAG AGATCCTCAG AAAGGGAGGA TGAAGAAAAA 
CAGAAAAAAA ATCCTCCTTG GCTCTCTCTG GTTCCTGCGA CAGAGCCCAA AAGGAGAGCT 
GCTCCCCCAC CTCCACCACT GGCTCAAAAG CCGGAGTTAG GAGATGAGAA ATCTCAGAAG 
TCTTTAAATG ACACAGAAAA GCAAAGTCTC TCATCAAAGC CAACAGCCTA CAATCCATTT 
GAAGATGAAG AAGAGGAAGC GGAAACAAGT TCTCTAAAAC CAGGCCACCC ATGGTATGGA 
ATTACACCAA CCAGCAGCCC CAAGAGTCGC AAAAGGCTAG CACCTAAAGC TCCCAATGCT 
TCACCTCTGG CTCATCATTC TAACCTAGTG TCAAGACTGT CTCACTCGGA GCCTCCCTCG 
GGCAGCCCTT CCCCAGCCCT AAGTACAGAG AGCCTTCCAA CGGATGCTTC CACAAAGGGC 
CAGGATCCTG AGAGTGTTCC CAAAAGCTCA TCTGAACCAA CTATCCATTC TCCAAACAAG 
CCAGAACCTT CAGATAGAGA TACTACTACC TACCTCAGCA CTGGTGTCCC CAGTACTTCT 
ACCAACACCA GCCTTTCTTC CTCTAGCGAA TTAGCTAATA CTAATGAAGC ACAAGAAAGC 
CCATCCCCAG AAATGCCTCT TAGTCCAAGC CGACCTGCCC CTCAAGTACC TACCATCTCC 
ATTCCTAGCA CAGTGGCATC AGACAGCACA CCTCCAGAGA AAAAGCCTCT AGCAAAGCAA 
ATTTGCAAAC AGAATCCCTT TAATAGGAAA TCCTCTCCTG CTACATCTCC CATCAACAAA 
AGGAACCAGA AAGGTCCAAA ACCTTCCCGA CCCCCTGCTC CAGGACATGG GTTCCCCCTT 
ATAAAACGCA AGGTTCAGGC TGATGACTAC ATTCCGGAAG ATGAGATTCA GATGGAGATG 
GAGACAATTG AGCTGCGCCT GGATGAGCTA GAAAGGCGTG GGGTGGAGCT GGAACACAGA 
CTGCGCAAGT TGGAGAATGA GTCAGATGAA GATAGTCTGC TGGTGGACTG GTTCAAGCTG 
ATCCAAGAAA AACACACCCT GGTGCGCCGG GAGTCAGAGC TTGTGTACAC CACTAAACAA 
CAGAGCCTGG AACAGCGTCA GGCTGATGTG GAGTATGAAC TTCGCTGCTT ACTGAATAAG 
CCAGAAAAGG ACTGGCTGGA AGAGGATAAG GAACGGGAAC AAGTGCTAAT GCAGGAGCTG 
GTCACACTAA TTGAGCAGAG AAATGCTATT GTCAAGTGCT TGGATGAAGA CAGACAGAGG 
GAAAAAGAAG AGGACAAGAT CATTGAAGCT ATGATACTGA AGAAAGATTT TCATACAGAT 
CCGCAAAAAA AAAAGAAAGG AAAATTTAAA CCTATAAAAG TTCTTAAGCT TCTTTCTCCA 
AAGCAAGAGC CCAAAGTAAA AAGCCCTAAG GACAAAAACA AAGTGCAATC GGATGGGACC 
AAGAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756945.1
CDS	201..2609
Translation	MSASRNLQQWCSRQCEGYPGILIYDLSSSFRDGLAFCALIHRHRPDLIDFDSLSKENVFENNRLAFEVAERELGIPALLDPEDMVSMKVPDRLSIMTYLSQYYNHFRNSSPVGKPQLKETSESPSKEPSGGSLPQGSPNAQTSLIRSSISSTCALCQQHVHLVQRYLVEGKLYHRQCFRCKECSSTLVPGAYRPGTDAGTLVCTHHCHRPPTSSPESEEDGTKQKAVQTPPKPFVPEKPASSNKPPTELHPDPFPNMRPVPAPRRTSDVAPQPLPRSRAAAESSPVRPTSLINGEQQSPAPPIPKPRSRQRSSEREDEEKQKKNPPWLSLVPATEPKRRAAPPPPPLAQKPELGDEKSQKSLNDTEKQSLSSKPTAYNPFEDEEEEAETSSLKPGHPWYGITPTSSPKSRKRLAPKAPNASPLAHHSNLVSRLSHSEPPSGSPSPALSTESLPTDASTKGQDPESVPKSSSEPTIHSPNKPEPSDRDTTTYLSTGVPSTSTNTSLSSSSELANTNEAQESPSPEMPLSPSRPAPQVPTISIPSTVASDSTPPEKKPLAKQICKQNPFNRKSSPATSPINKRNQKGPKPSRPPAPGHGFPLIKRKVQADDYIPEDEIQMEMETIELRLDELERRGVELEHRLRKLENESDEDSLLVDWFKLIQEKHTLVRRESELVYTTKQQSLEQRQADVEYELRCLLNKPEKDWLEEDKEREQVLMQELVTLIEQRNAIVKCLDEDRQREKEEDKIIEAMILKKDFHTDPQKKKKGKFKPIKVLKLLSPKQEPKVKSPKDKNKVQSDGTKR

Target ORF information:

RefSeq Version	XM_031901085.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MICAL like 1 (micall1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901085.1

ORF Insert Sequence:

ATGTCGGCCT CACGGAATCT ACAGCAGTGG TGCTCCCGGC AGTGTGAGGG TTACCCTGGC 
ATTCTGATCT ATGACCTCAG CTCATCTTTT CGGGATGGAC TGGCCTTCTG CGCTCTCATT 
CACCGCCATC GGCCTGATCT CATAGACTTT GATTCTCTTT CTAAAGAAAA TGTCTTCGAG 
AACAACCGCC TGGCTTTTGA AGTGGCTGAA CGGGAACTGG GTATTCCAGC TTTGCTTGAT 
CCAGAGGACA TGGTGTCCAT GAAAGTGCCT GATCGACTGA GCATCATGAC GTACTTATCC 
CAGTATTATA ACCACTTCCG GAATTCTAGT CCAGTGGGAA AGCCTCAGCT GAAAGAAACC 
TCTGAGTCCC CCAGCAAAGA GCCCAGTGGA GGTTCCTTAC CTCAGGGAAG CCCAAATGCC 
CAGACCTCTT TGATCCGTAG CTCAATAAGC AGCACCTGTG CTCTGTGCCA GCAGCATGTA 
CACCTAGTGC AGAGATACCT GGTAGAGGGG AAGCTTTATC ACCGCCAGTG CTTCAGATGT 
AAAGAATGCT CAAGTACCCT AGTTCCAGGA GCCTACCGCC CAGGCACTGA TGCCGGCACT 
CTGGTTTGCA CTCACCACTG CCACCGGCCA CCAACCAGCA GCCCTGAATC TGAAGAAGAT 
GGCACTAAGC AAAAAGCAGT TCAGACCCCT CCCAAACCAT TTGTGCCAGA AAAACCTGCC 
TCCTCCAATA AACCTCCAAC AGAATTGCAT CCTGATCCCT TTCCTAACAT GCGACCAGTA 
CCAGCTCCAA GAAGAACAAG TGATGTGGCC CCTCAGCCTC TGCCACGCTC CCGTGCAGCA 
GCAGAATCAA GCCCAGTGAG GCCTACATCT CTAATAAATG GCGAACAACA GTCTCCCGCT 
CCACCAATAC CCAAACCAAG AAGCAGACAG AGATCCTCAG AAAGGGAGGA TGAAGAAAAA 
CAGAAAAAAA ATCCTCCTTG GCTCTCTCTG GTTCCTGCGA CAGAGCCCAA AAGGAGAGCT 
GCTCCCCCAC CTCCACCACT GGCTCAAAAG CCGGAGTTAG GAGATGAGAA ATCTCAGAAG 
TCTTTAAATG ACACAGAAAA GCAAAGTCTC TCATCAAAGC CAACAGCCTA CAATCCATTT 
GAAGATGAAG AAGAGGAAGC GGAAACAAGT TCTCTAAAAC CAGGCCACCC ATGGTATGGA 
ATTACACCAA CCAGCAGCCC CAAGAGTCGC AAAAGGCTAG CACCTAAAGC TCCCAATGCT 
TCACCTCTGG CTCATCATTC TAACCTAGTG TCAAGACTGT CTCACTCGGA GCCTCCCTCG 
GGCAGCCCTT CCCCAGCCCT AAGTACAGAG AGCCTTCCAA CGGATGCTTC CACAAAGGGC 
CAGGATCCTG AGAGTGTTCC CAAAAGCTCA TCTGAACCAA CTATCCATTC TCCAAACAAG 
CCAGAACCTT CAGATAGAGA TACTACTACC TACCTCAGCA CTGGTGTCCC CAGTACTTCT 
ACCAACACCA GCCTTTCTTC CTCTAGCGAA TTAGCTAATA CTAATGAAGC ACAAGAAAGC 
CCATCCCCAG AAATGCCTCT TAGTCCAAGC CGACCTGCCC CTCAAGTACC TACCATCTCC 
ATTCCTAGCA CAGTGGCATC AGACAGCACA CCTCCAGAGA AAAAGCCTCT AGCAAAGCAA 
ATTTGCAAAC AGAATCCCTT TAATAGGAAA TCCTCTCCTG CTACATCTCC CATCAACAAA 
AGGAACCAGA AAGGTCCAAA ACCTTCCCGA CCCCCTGCTC CAGGACATGG GTTCCCCCTT 
ATAAAACGCA AGGTTCAGGC TGATGACTAC ATTCCGGAAG ATGAGATTCA GATGGAGATG 
GAGACAATTG AGCTGCGCCT GGATGAGCTA GAAAGGCGTG GGGTGGAGCT GGAACACAGA 
CTGCGCAAGT TGGAGAATGA GTCAGATGAA GATAGTCTGC TGGTGGACTG GTTCAAGCTG 
ATCCAAGAAA AACACACCCT GGTGCGCCGG GAGTCAGAGC TTGTGTACAC CACTAAACAA 
CAGAGCCTGG AACAGCGTCA GGCTGATGTG GAGTATGAAC TTCGCTGCTT ACTGAATAAG 
CCAGAAAAGG ACTGGCTGGA AGAGGATAAG GAACGGGAAC AAGTGCTAAT GCAGGAGCTG 
GTCACACTAA TTGAGCAGAG AAATGCTATT GTCAAGTGCT TGGATGAAGA CAGACAGAGG 
GAAAAAGAAG AGGACAAGAT CATTGAAGCT ATGATACTGA AGAAAGATTT TCATACAGAT 
CCGCAAAAAA AAAAGAAAGG AAAATTTAAA CCTATAAAAG TTCTTAAGCT TCTTTCTCCA 
AAGCAAGAGC CCAAAGTAAA AAGCCCTAAG GACAAAAACA AAGTGCAATC GGATGGGACC 
AAGAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

micall1 ( XM_002934234.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934280.2 Xenopus tropicalis MICAL like 1 (micall1), transcript variant X1, mRNA.

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CloneID	OXa09312
Clone ID Related Accession (Same CDS sequence)	XM_031901085.1 , XM_002934234.4 , XM_002934234.5
Accession Version	XM_002934234.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MICAL-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934234.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCT CACGGAATCT ACAGCAGTGG TGCTCCCGGC AGTGTGAGGG TTACCCTGGC 
ATTCTGATCT ATGACCTCAG CTCATCTTTT CGGGATGGAC TGGCCTTCTG CGCTCTCATT 
CACCGCCATC GGCCTGATCT CATAGACTTT GATTCTCTTT CTAAAGAAAA TGTCTTCGAG 
AACAACCGCC TGGCTTTTGA AGTGGCTGAA CGGGAACTGG GTATTCCAGC TTTGCTTGAT 
CCAGAGGACA TGGTGTCCAT GAAAGTGCCT GATCGACTGA GCATCATGAC GTACTTATCC 
CAGTATTATA ACCACTTCCG GAATTCTAGT CCAGTGGGAA AGCCTCAGCT GAAAGAAACC 
TCTGAGTCCC CCAGCAAAGA GCCCAGTGGA GGTTCCTTAC CTCAGGGAAG CCCAAATGCC 
CAGACCTCTT TGATCCGTAG CTCAATAAGC AGCACCTGTG CTCTGTGCCA GCAGCATGTA 
CACCTAGTGC AGAGATACCT GGTAGAGGGG AAGCTTTATC ACCGCCAGTG CTTCAGATGT 
AAAGAATGCT CAAGTACCCT AGTTCCAGGA GCCTACCGCC CAGGCACTGA TGCCGGCACT 
CTGGTTTGCA CTCACCACTG CCACCGGCCA CCAACCAGCA GCCCTGAATC TGAAGAAGAT 
GGCACTAAGC AAAAAGCAGT TCAGACCCCT CCCAAACCAT TTGTGCCAGA AAAACCTGCC 
TCCTCCAATA AACCTCCAAC AGAATTGCAT CCTGATCCCT TTCCTAACAT GCGACCAGTA 
CCAGCTCCAA GAAGAACAAG TGATGTGGCC CCTCAGCCTC TGCCACGCTC CCGTGCAGCA 
GCAGAATCAA GCCCAGTGAG GCCTACATCT CTAATAAATG GCGAACAACA GTCTCCCGCT 
CCACCAATAC CCAAACCAAG AAGCAGACAG AGATCCTCAG AAAGGGAGGA TGAAGAAAAA 
CAGAAAAAAA ATCCTCCTTG GCTCTCTCTG GTTCCTGCGA CAGAGCCCAA AAGGAGAGCT 
GCTCCCCCAC CTCCACCACT GGCTCAAAAG CCGGAGTTAG GAGATGAGAA ATCTCAGAAG 
TCTTTAAATG ACACAGAAAA GCAAAGTCTC TCATCAAAGC CAACAGCCTA CAATCCATTT 
GAAGATGAAG AAGAGGAAGC GGAAACAAGT TCTCTAAAAC CAGGCCACCC ATGGTATGGA 
ATTACACCAA CCAGCAGCCC CAAGAGTCGC AAAAGGCTAG CACCTAAAGC TCCCAATGCT 
TCACCTCTGG CTCATCATTC TAACCTAGTG TCAAGACTGT CTCACTCGGA GCCTCCCTCG 
GGCAGCCCTT CCCCAGCCCT AAGTACAGAG AGCCTTCCAA CGGATGCTTC CACAAAGGGC 
CAGGATCCTG AGAGTGTTCC CAAAAGCTCA TCTGAACCAA CTATCCATTC TCCAAACAAG 
CCAGAACCTT CAGATAGAGA TACTACTACC TACCTCAGCA CTGGTGTCCC CAGTACTTCT 
ACCAACACCA GCCTTTCTTC CTCTAGCGAA TTAGCTAATA CTAATGAAGC ACAAGAAAGC 
CCATCCCCAG AAATGCCTCT TAGTCCAAGC CGACCTGCCC CTCAAGTACC TACCATCTCC 
ATTCCTAGCA CAGTGGCATC AGACAGCACA CCTCCAGAGA AAAAGCCTCT AGCAAAGCAA 
ATTTGCAAAC AGAATCCCTT TAATAGGAAA TCCTCTCCTG CTACATCTCC CATCAACAAA 
AGGAACCAGA AAGGTCCAAA ACCTTCCCGA CCCCCTGCTC CAGGACATGG GTTCCCCCTT 
ATAAAACGCA AGGTTCAGGC TGATGACTAC ATTCCGGAAG ATGAGATTCA GATGGAGATG 
GAGACAATTG AGCTGCGCCT GGATGAGCTA GAAAGGCGTG GGGTGGAGCT GGAACACAGA 
CTGCGCAAGT TGGAGAATGA GTCAGATGAA GATAGTCTGC TGGTGGACTG GTTCAAGCTG 
ATCCAAGAAA AACACACCCT GGTGCGCCGG GAGTCAGAGC TTGTGTACAC CACTAAACAA 
CAGAGCCTGG AACAGCGTCA GGCTGATGTG GAGTATGAAC TTCGCTGCTT ACTGAATAAG 
CCAGAAAAGG ACTGGCTGGA AGAGGATAAG GAACGGGAAC AAGTGCTAAT GCAGGAGCTG 
GTCACACTAA TTGAGCAGAG AAATGCTATT GTCAAGTGCT TGGATGAAGA CAGACAGAGG 
GAAAAAGAAG AGGACAAGAT CATTGAAGCT ATGATACTGA AGAAAGATTT TCATACAGAT 
CCGCAAAAAA AAAAGAAAGG AAAATTTAAA CCTATAAAAG TTCTTAAGCT TCTTTCTCCA 
AAGCAAGAGC CCAAAGTAAA AAGCCCTAAG GACAAAAACA AAGTGCAATC GGATGGGACC 
AAGAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934280.2
CDS	201..2609
Translation	MSASRNLQQWCSRQCEGYPGILIYDLSSSFRDGLAFCALIHRHRPDLIDFDSLSKENVFENNRLAFEVAERELGIPALLDPEDMVSMKVPDRLSIMTYLSQYYNHFRNSSPVGKPQLKETSESPSKEPSGGSLPQGSPNAQTSLIRSSISSTCALCQQHVHLVQRYLVEGKLYHRQCFRCKECSSTLVPGAYRPGTDAGTLVCTHHCHRPPTSSPESEEDGTKQKAVQTPPKPFVPEKPASSNKPPTELHPDPFPNMRPVPAPRRTSDVAPQPLPRSRAAAESSPVRPTSLINGEQQSPAPPIPKPRSRQRSSEREDEEKQKKNPPWLSLVPATEPKRRAAPPPPPLAQKPELGDEKSQKSLNDTEKQSLSSKPTAYNPFEDEEEEAETSSLKPGHPWYGITPTSSPKSRKRLAPKAPNASPLAHHSNLVSRLSHSEPPSGSPSPALSTESLPTDASTKGQDPESVPKSSSEPTIHSPNKPEPSDRDTTTYLSTGVPSTSTNTSLSSSSELANTNEAQESPSPEMPLSPSRPAPQVPTISIPSTVASDSTPPEKKPLAKQICKQNPFNRKSSPATSPINKRNQKGPKPSRPPAPGHGFPLIKRKVQADDYIPEDEIQMEMETIELRLDELERRGVELEHRLRKLENESDEDSLLVDWFKLIQEKHTLVRRESELVYTTKQQSLEQRQADVEYELRCLLNKPEKDWLEEDKEREQVLMQELVTLIEQRNAIVKCLDEDRQREKEEDKIIEAMILKKDFHTDPQKKKKGKFKPIKVLKLLSPKQEPKVKSPKDKNKVQSDGTKR

Target ORF information:

RefSeq Version	XM_002934234.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MICAL like 1 (micall1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934234.5

ORF Insert Sequence:

ATGTCGGCCT CACGGAATCT ACAGCAGTGG TGCTCCCGGC AGTGTGAGGG TTACCCTGGC 
ATTCTGATCT ATGACCTCAG CTCATCTTTT CGGGATGGAC TGGCCTTCTG CGCTCTCATT 
CACCGCCATC GGCCTGATCT CATAGACTTT GATTCTCTTT CTAAAGAAAA TGTCTTCGAG 
AACAACCGCC TGGCTTTTGA AGTGGCTGAA CGGGAACTGG GTATTCCAGC TTTGCTTGAT 
CCAGAGGACA TGGTGTCCAT GAAAGTGCCT GATCGACTGA GCATCATGAC GTACTTATCC 
CAGTATTATA ACCACTTCCG GAATTCTAGT CCAGTGGGAA AGCCTCAGCT GAAAGAAACC 
TCTGAGTCCC CCAGCAAAGA GCCCAGTGGA GGTTCCTTAC CTCAGGGAAG CCCAAATGCC 
CAGACCTCTT TGATCCGTAG CTCAATAAGC AGCACCTGTG CTCTGTGCCA GCAGCATGTA 
CACCTAGTGC AGAGATACCT GGTAGAGGGG AAGCTTTATC ACCGCCAGTG CTTCAGATGT 
AAAGAATGCT CAAGTACCCT AGTTCCAGGA GCCTACCGCC CAGGCACTGA TGCCGGCACT 
CTGGTTTGCA CTCACCACTG CCACCGGCCA CCAACCAGCA GCCCTGAATC TGAAGAAGAT 
GGCACTAAGC AAAAAGCAGT TCAGACCCCT CCCAAACCAT TTGTGCCAGA AAAACCTGCC 
TCCTCCAATA AACCTCCAAC AGAATTGCAT CCTGATCCCT TTCCTAACAT GCGACCAGTA 
CCAGCTCCAA GAAGAACAAG TGATGTGGCC CCTCAGCCTC TGCCACGCTC CCGTGCAGCA 
GCAGAATCAA GCCCAGTGAG GCCTACATCT CTAATAAATG GCGAACAACA GTCTCCCGCT 
CCACCAATAC CCAAACCAAG AAGCAGACAG AGATCCTCAG AAAGGGAGGA TGAAGAAAAA 
CAGAAAAAAA ATCCTCCTTG GCTCTCTCTG GTTCCTGCGA CAGAGCCCAA AAGGAGAGCT 
GCTCCCCCAC CTCCACCACT GGCTCAAAAG CCGGAGTTAG GAGATGAGAA ATCTCAGAAG 
TCTTTAAATG ACACAGAAAA GCAAAGTCTC TCATCAAAGC CAACAGCCTA CAATCCATTT 
GAAGATGAAG AAGAGGAAGC GGAAACAAGT TCTCTAAAAC CAGGCCACCC ATGGTATGGA 
ATTACACCAA CCAGCAGCCC CAAGAGTCGC AAAAGGCTAG CACCTAAAGC TCCCAATGCT 
TCACCTCTGG CTCATCATTC TAACCTAGTG TCAAGACTGT CTCACTCGGA GCCTCCCTCG 
GGCAGCCCTT CCCCAGCCCT AAGTACAGAG AGCCTTCCAA CGGATGCTTC CACAAAGGGC 
CAGGATCCTG AGAGTGTTCC CAAAAGCTCA TCTGAACCAA CTATCCATTC TCCAAACAAG 
CCAGAACCTT CAGATAGAGA TACTACTACC TACCTCAGCA CTGGTGTCCC CAGTACTTCT 
ACCAACACCA GCCTTTCTTC CTCTAGCGAA TTAGCTAATA CTAATGAAGC ACAAGAAAGC 
CCATCCCCAG AAATGCCTCT TAGTCCAAGC CGACCTGCCC CTCAAGTACC TACCATCTCC 
ATTCCTAGCA CAGTGGCATC AGACAGCACA CCTCCAGAGA AAAAGCCTCT AGCAAAGCAA 
ATTTGCAAAC AGAATCCCTT TAATAGGAAA TCCTCTCCTG CTACATCTCC CATCAACAAA 
AGGAACCAGA AAGGTCCAAA ACCTTCCCGA CCCCCTGCTC CAGGACATGG GTTCCCCCTT 
ATAAAACGCA AGGTTCAGGC TGATGACTAC ATTCCGGAAG ATGAGATTCA GATGGAGATG 
GAGACAATTG AGCTGCGCCT GGATGAGCTA GAAAGGCGTG GGGTGGAGCT GGAACACAGA 
CTGCGCAAGT TGGAGAATGA GTCAGATGAA GATAGTCTGC TGGTGGACTG GTTCAAGCTG 
ATCCAAGAAA AACACACCCT GGTGCGCCGG GAGTCAGAGC TTGTGTACAC CACTAAACAA 
CAGAGCCTGG AACAGCGTCA GGCTGATGTG GAGTATGAAC TTCGCTGCTT ACTGAATAAG 
CCAGAAAAGG ACTGGCTGGA AGAGGATAAG GAACGGGAAC AAGTGCTAAT GCAGGAGCTG 
GTCACACTAA TTGAGCAGAG AAATGCTATT GTCAAGTGCT TGGATGAAGA CAGACAGAGG 
GAAAAAGAAG AGGACAAGAT CATTGAAGCT ATGATACTGA AGAAAGATTT TCATACAGAT 
CCGCAAAAAA AAAAGAAAGG AAAATTTAAA CCTATAAAAG TTCTTAAGCT TCTTTCTCCA 
AAGCAAGAGC CCAAAGTAAA AAGCCCTAAG GACAAAAACA AAGTGCAATC GGATGGGACC 
AAGAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

micall1 ( XM_018093657.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949146.1 Xenopus tropicalis MICAL like 1 (micall1), transcript variant X2, mRNA.

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CloneID	OXa44227
Clone ID Related Accession (Same CDS sequence)	XM_018093657.1
Accession Version	XM_018093657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MICAL-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCCT CACGGAATCT ACAGCAGTGG TGCTCCCGGC AGTGTGAGGG TTACCCTGGC 
ATTCTGATCT ATGACCTCAG CTCATCTTTT CGGGATGGAC TGGCCTTCTG CGCTCTCATT 
CACCGCCATC GGCCTGATCT CATAGACTTT GATTCTCTTT CTAAAGAAAA TGTCTTCGAG 
AACAACCGCC TGGCTTTTGA AGTGGCTGAA CGGGAACTGG GTATTCCAGC TTTGCTTGAT 
CCAGAGGACA TGGTGTCCAT GAAAGTGCCT GATCGACTGA GCATCATGAC GTACTTATCC 
CAGTATTATA ACCACTTCCG GAATTCTAGT CCAGTGGGAA AGCCTCAGCT GAAAGAAACC 
TCTGAGTCCC CCAGCAAAGA GCCCAGTGGA GGTTCCTTAC CTCAGGGAAG CCCAAATGCC 
CAGACCTCTT TGATCCGTAG CTCAATAAGC AGCACCTGTG CTCTGTGCCA GCAGCATGTA 
CACCTAGTGC AGAGATACCT GGTAGAGGGG AAGCTTTATC ACCGCCAGTG CTTCAGATGT 
AAAGAATGCT CAAGTACCCT AGTTCCAGGA GCCTACCGCC CAGGCACTGA TGCCGGCACT 
CTGGTTTGCA CTCACCACTG CCACCGGCCA CCAACCAGCA GCCCTGAATC TGAAGAAGAT 
GGCACTAAGC AAAAAGCAGT TCAGACCCCT CCCAAACCAT TTGTGCCAGA AAAACCTGCC 
TCCTCCAATA AACCTCCAAC AGAATTGCAT CCTGATCCCT TTCCTAACAT GCGACCAGTA 
CCAGCTCCAA GAAGAACAAG TGATGTGGCC CCTCAGCCTC TGCCACGCTC CCGTGCAGCA 
GCAGAATCAA GCCCAGTGAG GCCTACATCT CTAATAAATG GCGAACAACA GTCTCCCGCT 
CCACCAATAC CCAAACCAAG AAGCAGACAG AGATCCTCAG AAAGGGAGGA TGAAGAAAAA 
CAGAAAAAAA ATCCTCCTTG GCTCTCTCTG GTTCCTGCGA CAGAGCCCAA AAGGAGAGCT 
GCTCCCCCAC CTCCACCACT GGCTCAAAAG CCGGAGTTAG GAGATGAGAA ATCTCAGAAG 
TCTTTAAATG ACACAGAAAA GCAAAGTCTC TCATCAAAGC CAACAGCCTA CAATCCATTT 
GAAGATGAAG AAGAGGAAGC GGAAACAAGT TCTCTAAAAC CAGGCCACCC ATGGTATGGA 
ATTACACCAA CCAGCAGCCC CAAGAGTCGC AAAAGGCTAG CACCTAAAGC TCCCAATGCT 
TCACCTCTGG CTCATCATTC TAACCTAGTG TCAAGACTGT CTCACTCGGA GCCTCCCTCG 
GGCAGCCCTT CCCCAGCCCT AAGTACAGAG AGCCTTCCAA CGGATGCTTC CACAAAGGGC 
CAGGATCCTG AGAGTGTTCC CAAAAGCTCA TCTGAACCAA CTATCCATTC TCCAAACAAG 
CCAGAACCTT CAGATAGAGA TACTACTACC TACCTCAGCA CTGGTGTCCC CAGTACTTCT 
ACCAACACCA GCCTTTCTTC CTCTAGCGAA TTAGCTAATA CTAATGAAGC ACAAGAAAGC 
CCATCCCCAG AAATGCCTCT TAGTCCAAGC CGACCTGCCC CTCAAGTACC TACCATCTCC 
ATTCCTAGCA CAGTGGCATC AGACAGCACA CCTCCAGAGA AAAAGCCTCT AGCAAAGCAA 
ATTTGCAAAC AGAATCCCTT TAATAGGAAA TCCTCTCCTG CTACATCTCC CATCAACAAA 
AGGAACCAGA AAGGTCCAAA ACCTTCCCGA CCCCCTGCTC CAGGACATGG GTTCCCCCTT 
ATAAAACGCA AGGTTCAGGC TGATGACTAC ATTCCGGAAG ATGAGATTCA GATGGAGATG 
GAGACAATTG AGCTGCGCCT GGATGAGCTA GAAAGGCGTG GGGTGGAGCT GGAACACAGA 
CTGCGCAAGT TGGAGAATGC ACTAAACAAC AGAGCCTGGA ACAGCGTCAG GCTGATGTGG 
AGTATGAACT TCGCTGCTTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949146.1
CDS	217..2220
Translation	MSASRNLQQWCSRQCEGYPGILIYDLSSSFRDGLAFCALIHRHRPDLIDFDSLSKENVFENNRLAFEVAERELGIPALLDPEDMVSMKVPDRLSIMTYLSQYYNHFRNSSPVGKPQLKETSESPSKEPSGGSLPQGSPNAQTSLIRSSISSTCALCQQHVHLVQRYLVEGKLYHRQCFRCKECSSTLVPGAYRPGTDAGTLVCTHHCHRPPTSSPESEEDGTKQKAVQTPPKPFVPEKPASSNKPPTELHPDPFPNMRPVPAPRRTSDVAPQPLPRSRAAAESSPVRPTSLINGEQQSPAPPIPKPRSRQRSSEREDEEKQKKNPPWLSLVPATEPKRRAAPPPPPLAQKPELGDEKSQKSLNDTEKQSLSSKPTAYNPFEDEEEEAETSSLKPGHPWYGITPTSSPKSRKRLAPKAPNASPLAHHSNLVSRLSHSEPPSGSPSPALSTESLPTDASTKGQDPESVPKSSSEPTIHSPNKPEPSDRDTTTYLSTGVPSTSTNTSLSSSSELANTNEAQESPSPEMPLSPSRPAPQVPTISIPSTVASDSTPPEKKPLAKQICKQNPFNRKSSPATSPINKRNQKGPKPSRPPAPGHGFPLIKRKVQADDYIPEDEIQMEMETIELRLDELERRGVELEHRLRKLENALNNRAWNSVRLMWSMNFAAY

Target ORF information:

RefSeq Version	XM_018093657.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MICAL like 1 (micall1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093657.1

ORF Insert Sequence:

ATGTCGGCCT CACGGAATCT ACAGCAGTGG TGCTCCCGGC AGTGTGAGGG TTACCCTGGC 
ATTCTGATCT ATGACCTCAG CTCATCTTTT CGGGATGGAC TGGCCTTCTG CGCTCTCATT 
CACCGCCATC GGCCTGATCT CATAGACTTT GATTCTCTTT CTAAAGAAAA TGTCTTCGAG 
AACAACCGCC TGGCTTTTGA AGTGGCTGAA CGGGAACTGG GTATTCCAGC TTTGCTTGAT 
CCAGAGGACA TGGTGTCCAT GAAAGTGCCT GATCGACTGA GCATCATGAC GTACTTATCC 
CAGTATTATA ACCACTTCCG GAATTCTAGT CCAGTGGGAA AGCCTCAGCT GAAAGAAACC 
TCTGAGTCCC CCAGCAAAGA GCCCAGTGGA GGTTCCTTAC CTCAGGGAAG CCCAAATGCC 
CAGACCTCTT TGATCCGTAG CTCAATAAGC AGCACCTGTG CTCTGTGCCA GCAGCATGTA 
CACCTAGTGC AGAGATACCT GGTAGAGGGG AAGCTTTATC ACCGCCAGTG CTTCAGATGT 
AAAGAATGCT CAAGTACCCT AGTTCCAGGA GCCTACCGCC CAGGCACTGA TGCCGGCACT 
CTGGTTTGCA CTCACCACTG CCACCGGCCA CCAACCAGCA GCCCTGAATC TGAAGAAGAT 
GGCACTAAGC AAAAAGCAGT TCAGACCCCT CCCAAACCAT TTGTGCCAGA AAAACCTGCC 
TCCTCCAATA AACCTCCAAC AGAATTGCAT CCTGATCCCT TTCCTAACAT GCGACCAGTA 
CCAGCTCCAA GAAGAACAAG TGATGTGGCC CCTCAGCCTC TGCCACGCTC CCGTGCAGCA 
GCAGAATCAA GCCCAGTGAG GCCTACATCT CTAATAAATG GCGAACAACA GTCTCCCGCT 
CCACCAATAC CCAAACCAAG AAGCAGACAG AGATCCTCAG AAAGGGAGGA TGAAGAAAAA 
CAGAAAAAAA ATCCTCCTTG GCTCTCTCTG GTTCCTGCGA CAGAGCCCAA AAGGAGAGCT 
GCTCCCCCAC CTCCACCACT GGCTCAAAAG CCGGAGTTAG GAGATGAGAA ATCTCAGAAG 
TCTTTAAATG ACACAGAAAA GCAAAGTCTC TCATCAAAGC CAACAGCCTA CAATCCATTT 
GAAGATGAAG AAGAGGAAGC GGAAACAAGT TCTCTAAAAC CAGGCCACCC ATGGTATGGA 
ATTACACCAA CCAGCAGCCC CAAGAGTCGC AAAAGGCTAG CACCTAAAGC TCCCAATGCT 
TCACCTCTGG CTCATCATTC TAACCTAGTG TCAAGACTGT CTCACTCGGA GCCTCCCTCG 
GGCAGCCCTT CCCCAGCCCT AAGTACAGAG AGCCTTCCAA CGGATGCTTC CACAAAGGGC 
CAGGATCCTG AGAGTGTTCC CAAAAGCTCA TCTGAACCAA CTATCCATTC TCCAAACAAG 
CCAGAACCTT CAGATAGAGA TACTACTACC TACCTCAGCA CTGGTGTCCC CAGTACTTCT 
ACCAACACCA GCCTTTCTTC CTCTAGCGAA TTAGCTAATA CTAATGAAGC ACAAGAAAGC 
CCATCCCCAG AAATGCCTCT TAGTCCAAGC CGACCTGCCC CTCAAGTACC TACCATCTCC 
ATTCCTAGCA CAGTGGCATC AGACAGCACA CCTCCAGAGA AAAAGCCTCT AGCAAAGCAA 
ATTTGCAAAC AGAATCCCTT TAATAGGAAA TCCTCTCCTG CTACATCTCC CATCAACAAA 
AGGAACCAGA AAGGTCCAAA ACCTTCCCGA CCCCCTGCTC CAGGACATGG GTTCCCCCTT 
ATAAAACGCA AGGTTCAGGC TGATGACTAC ATTCCGGAAG ATGAGATTCA GATGGAGATG 
GAGACAATTG AGCTGCGCCT GGATGAGCTA GAAAGGCGTG GGGTGGAGCT GGAACACAGA 
CTGCGCAAGT TGGAGAATGC ACTAAACAAC AGAGCCTGGA ACAGCGTCAG GCTGATGTGG 
AGTATGAACT TCGCTGCTTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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micall1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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