kdm6b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	kdm6b
Entrez Gene ID	779446
Full Name	lysine (K)-specific demethylase 6B
Synonyms	jmjd3,xtjmjd3
General protein information	Preferred Names lysine (K)-specific demethylase 6B Names lysine-specific demethylase 6B jumonji domain containing 3 jumonji domain-containing 3 histone lysine demethylase kdm6b
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031898015.1	XP_031753875.1	lysine-specific demethylase 6B isoform X1
NM_001278756.1	NP_001265685.1	lysine-specific demethylase 6B
XM_031898018.1	XP_031753878.1	lysine-specific demethylase 6B isoform X2
XM_031898019.1	XP_031753879.1	lysine-specific demethylase 6B isoform X3
XM_031898020.1	XP_031753880.1	lysine-specific demethylase 6B isoform X3
XM_031898021.1	XP_031753881.1	lysine-specific demethylase 6B isoform X4
XM_031898016.1	XP_031753876.1	lysine-specific demethylase 6B isoform X1
XM_031898017.1	XP_031753877.1	lysine-specific demethylase 6B isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparative expression analysis of the H3K27 demethylases, JMJD3 and UTX, with the H3K27 methylase, EZH2, in Xenopus.
Kawaguchi A, Ochi H, Sudou N, Ogino H
The International journal of developmental biology56(4)295-300(2012)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that at the blastula stage, jmjd3, utx and ezh2 showed similar expression patterns in the animal cap and marginal zone that give rise to the ectoderm and mesoderm, respectively.
Title: Comparative expression analysis of the H3K27 demethylases, JMJD3 and UTX, with the H3K27 methylase, EZH2, in Xenopus.

The following kdm6b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kdm6b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa53612	XM_031898016.1 Latest version!	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa53612	XM_031898017.1 Latest version!	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa53612	XM_031898015.1 Latest version!	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa53613	XM_031898018.1 Latest version!	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa53614	XM_031898019.1 Latest version!	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa53614	XM_031898020.1 Latest version!	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa53615	XM_031898021.1 Latest version!	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa20376	NM_001278756.1 Latest version!	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kdm6b ( XM_031898015.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753875.1 Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa53612
Clone ID Related Accession (Same CDS sequence)	XM_031898015.1 , XM_031898016.1 , XM_031898017.1
Accession Version	XM_031898015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT 
GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG 
CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA 
CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT 
CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG 
CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC 
AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA 
ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA 
CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA 
GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG 
GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC 
CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC 
CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA 
GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA 
CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA 
GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA 
GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT 
CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG 
AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG 
CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG 
AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG 
GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC 
CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG 
GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA 
GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC 
GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA 
GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT 
ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC 
CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG 
ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG 
TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA 
GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG 
GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT 
ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC 
TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG 
ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT 
GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG 
AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT 
ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG 
GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT 
TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC 
GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC 
AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT 
GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG 
CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC 
CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG 
AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT 
ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT 
GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC 
ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG 
TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG 
AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG 
CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT 
ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC 
CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA 
CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT 
CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC 
TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG 
TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT 
ACCACAGAAA CCCCACCCCC CAGTAGCACA AACCCGGAGC ACAAGACCCC ACAGATTATC 
AAGTTCGGCA CCAATATTGA TCTGTCTGAT CCCAAAAGGT GGAAACCTCA GTTACAGGAG 
CTGCAGAAGT TGCCATCTTT CATGCGTGTT ACATCCAATG CCAACATGCT AAGCCACGTG 
GGCCATACTA TACTTGGCAT GAACACCGTT CAGCTCTACA TGAAGGTTCC GGGCAGCAGG 
ACCCCGGGTC ACCAGGAGAA TAACAACTTC TGCTCTGTGA ATATAAACAT CGGCCCCGGG 
GACTGCGAAT GGTTCGCTGT ACATGAAAAT TACTGGGAGA AAATCAGCGC CTTCTGTGAC 
AAACACAATC TGGACTACCT GACCGGATCT TGGTGGCCGG TTCTAGAAGA CCTGTACCGC 
GCCGACATCC CAGTTTATCG GTTTGTTCAG CGCCCGGGAG ACCTGGTCTG GATCAACGCC 
GGAACTGTGC ATTGGGTGCA GGCTACAGGC TGGTGCAACA ACATCGCGTG GAACGTGGGG 
CCCCTGACAC CCTATCAGTA CCAACTGGCG CTGGAGCGAT TTGAGTGGAA CGAAGTCAAG 
AACGTGAAAT CCATCGTCCC AATGATCCAC GTGTCCTGGA ACGTGGCCCG GACCGTCAAA 
ATCAGTGATC CAGACCTGTA TCGCATGATC AAGTACTGCC TGATGCAGTC TATGAAGCGC 
TGCGTTTTGC AGAGGGACAG CCTGATCCGC GGCGGGAAGA AAATCGCCTA CCAGAGCAGA 
GTGAAAGATG AACCGGCCTA TTACTGCAAC GAGTGTGATG TGGAGGTGTT TAACATCCTA 
TTCGTGACCA GCGAGAGCGG TGGGAAGAAC ACGTACCTGG TGCACTGTGA GAGCTGCTCC 
CGGAGCGCCA GTAGCGCAAG CAGCGCCAGC AGCAACGTCG TCATCTTGGA GCAGTTTAAG 
ACCGAGGAGC TAATGCACAT ATACGACAAC TTCCACCTGG CTCCACCCCC GAACATCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753875.1
CDS	542..5764
Translation	MYRSQDQFGSRFPRDSFISGVNTTWSPPHPRPWAPVSRCSSNMNQPQLPPYLPSGHVTNLGHPSKPYYPISSPPGPRIGKLERVHSCVQALLRDEQQPSLWEQLGQIYESEQEWEDAIHCYQTAARYHGGYELGARISRLQQAHMWNMHATPHHRPKSLPPLQQVWDLMQHEQKRHFGAKRCLPQLKRPVPPSDSSALQLSPHNPHGGEEQPMKRRRSRSPEQQRRGNPGLHSPPASGGSGSLYHQAPVKPSVWNPLTHVPWAERRERQEPRPPHAPSYPPYCPPRPLPPPPPLHQQPRPPSDLSEARGQRCAEAVPPAPPPLHVPYDQRRPRATPPTTPRTDLTTSSDRYQNSAGSPSRICPPLVEQLDPPQTICQPQLPAVSRPPPPLQQGWLPPPAKEEMKKGDPLEDILYGDGPTKVKQEGFYPSRDTQREGNVYNHQPQGITTSKARSEAYQPGSLDHVLQKMDESGLIAPKKEEEIKQEVRNHPLTPELEVGGKQSSHGLSRAEGSYSGGKGSPEGADETSFCPSLAPHHKDTSGSLPVPGSVALPAKPMPNLTRRPLSPNSAFSERTSVCGQEKEQPTGCNQLFEFPGGQLHEPFEEAAEFAKILPDGLDDIMKMLDESIEKEEEGVGNASGMDDSSSTTSSSPPAETMPASSLVSSNQTTVVKSPPPNAKTAEVRPSDTFPYHGPKKAMLEQEASKEAKTAQHRGGSVLKSLANVLEGQKYCYRGSSGAKSAGNPAYFTGAMVSKRNVDPYATQSTSHRAWDTKPGTHVGAAPTSEALKPGVIEKRPEVRTETDILEDIARACETLVEGAKGSAASRTGNERAVEKEPKKSEGIVDRKPDLQWGIEKRDIHWGANKLEGPVKQKVDFKWRSDTQKEVVEKKPEAPRATGQQDYVEKKHGADKPRVPVDRKPETLQKADVQEGVAEIKQETSYGTIRHEVAERLEAALERESKKSVKGAEKKCLPGPADSPSGTAREDPSKIHGANVKEAVKEATAKRLQGVNDRESPKKNTDSLAQGGVSKNVAAEVAKPNADSKDTKSSEEAPMPTMEAGDNGTKGWEAGKPIERLTISLPAPARSKEESRRRERKHRKSRRDCSKKSTDRRSLKDKERQVLGNLDLQSQCRDTSNSTLKQSSRKSETGRRGCEEAHHHQPTPPTPPTSAPPPTSHGTANSDLLKMRPFTEGPPKELKIRLIKVESGDRETFIASEVEERRLQASELTINNTAAEIVRATRSMVLKGKFKESYLLPSMSVKPPLGTEQNLPREKLNPPTPSIYLESKRDAFSPILLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKTLVDANGEHMVEVRTQVQQPSDENWDPSGTRQIWHCESSRSHTTIAKYAQYQASSFQESLQEDKESDDDSNEPDSTTETPPPSSTNPEHKTPQIIKFGTNIDLSDPKRWKPQLQELQKLPSFMRVTSNANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHENYWEKISAFCDKHNLDYLTGSWWPVLEDLYRADIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTPYQYQLALERFEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLYRMIKYCLMQSMKRCVLQRDSLIRGGKKIAYQSRVKDEPAYYCNECDVEVFNILFVTSESGGKNTYLVHCESCSRSASSASSASSNVVILEQFKTEELMHIYDNFHLAPPPNIR

Target ORF information:

RefSeq Version	XM_031898015.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898015.1

ORF Insert Sequence:

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT 
GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG 
CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA 
CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT 
CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG 
CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC 
AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA 
ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA 
CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA 
GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG 
GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC 
CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC 
CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA 
GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA 
CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA 
GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA 
GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT 
CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG 
AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG 
CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG 
AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG 
GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC 
CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG 
GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA 
GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC 
GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA 
GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT 
ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC 
CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG 
ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG 
TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA 
GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG 
GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT 
ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC 
TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG 
ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT 
GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG 
AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT 
ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG 
GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT 
TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC 
GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC 
AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT 
GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG 
CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC 
CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG 
AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT 
ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT 
GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC 
ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG 
TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG 
AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG 
CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT 
ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC 
CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA 
CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT 
CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC 
TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG 
TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT 
ACCACAGAAA CCCCACCCCC CAGTAGCACA AACCCGGAGC ACAAGACCCC ACAGATTATC 
AAGTTCGGCA CCAATATTGA TCTGTCTGAT CCCAAAAGGT GGAAACCTCA GTTACAGGAG 
CTGCAGAAGT TGCCATCTTT CATGCGTGTT ACATCCAATG CCAACATGCT AAGCCACGTG 
GGCCATACTA TACTTGGCAT GAACACCGTT CAGCTCTACA TGAAGGTTCC GGGCAGCAGG 
ACCCCGGGTC ACCAGGAGAA TAACAACTTC TGCTCTGTGA ATATAAACAT CGGCCCCGGG 
GACTGCGAAT GGTTCGCTGT ACATGAAAAT TACTGGGAGA AAATCAGCGC CTTCTGTGAC 
AAACACAATC TGGACTACCT GACCGGATCT TGGTGGCCGG TTCTAGAAGA CCTGTACCGC 
GCCGACATCC CAGTTTATCG GTTTGTTCAG CGCCCGGGAG ACCTGGTCTG GATCAACGCC 
GGAACTGTGC ATTGGGTGCA GGCTACAGGC TGGTGCAACA ACATCGCGTG GAACGTGGGG 
CCCCTGACAC CCTATCAGTA CCAACTGGCG CTGGAGCGAT TTGAGTGGAA CGAAGTCAAG 
AACGTGAAAT CCATCGTCCC AATGATCCAC GTGTCCTGGA ACGTGGCCCG GACCGTCAAA 
ATCAGTGATC CAGACCTGTA TCGCATGATC AAGTACTGCC TGATGCAGTC TATGAAGCGC 
TGCGTTTTGC AGAGGGACAG CCTGATCCGC GGCGGGAAGA AAATCGCCTA CCAGAGCAGA 
GTGAAAGATG AACCGGCCTA TTACTGCAAC GAGTGTGATG TGGAGGTGTT TAACATCCTA 
TTCGTGACCA GCGAGAGCGG TGGGAAGAAC ACGTACCTGG TGCACTGTGA GAGCTGCTCC 
CGGAGCGCCA GTAGCGCAAG CAGCGCCAGC AGCAACGTCG TCATCTTGGA GCAGTTTAAG 
ACCGAGGAGC TAATGCACAT ATACGACAAC TTCCACCTGG CTCCACCCCC GAACATCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6b ( XM_031898016.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753876.1 Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X2, mRNA.

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CloneID	OXa53612
Clone ID Related Accession (Same CDS sequence)	XM_031898015.1 , XM_031898016.1 , XM_031898017.1
Accession Version	XM_031898016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT 
GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG 
CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA 
CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT 
CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG 
CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC 
AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA 
ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA 
CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA 
GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG 
GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC 
CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC 
CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA 
GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA 
CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA 
GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA 
GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT 
CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG 
AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG 
CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG 
AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG 
GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC 
CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG 
GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA 
GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC 
GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA 
GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT 
ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC 
CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG 
ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG 
TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA 
GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG 
GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT 
ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC 
TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG 
ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT 
GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG 
AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT 
ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG 
GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT 
TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC 
GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC 
AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT 
GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG 
CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC 
CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG 
AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT 
ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT 
GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC 
ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG 
TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG 
AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG 
CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT 
ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC 
CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA 
CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT 
CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC 
TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG 
TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT 
ACCACAGAAA CCCCACCCCC CAGTAGCACA AACCCGGAGC ACAAGACCCC ACAGATTATC 
AAGTTCGGCA CCAATATTGA TCTGTCTGAT CCCAAAAGGT GGAAACCTCA GTTACAGGAG 
CTGCAGAAGT TGCCATCTTT CATGCGTGTT ACATCCAATG CCAACATGCT AAGCCACGTG 
GGCCATACTA TACTTGGCAT GAACACCGTT CAGCTCTACA TGAAGGTTCC GGGCAGCAGG 
ACCCCGGGTC ACCAGGAGAA TAACAACTTC TGCTCTGTGA ATATAAACAT CGGCCCCGGG 
GACTGCGAAT GGTTCGCTGT ACATGAAAAT TACTGGGAGA AAATCAGCGC CTTCTGTGAC 
AAACACAATC TGGACTACCT GACCGGATCT TGGTGGCCGG TTCTAGAAGA CCTGTACCGC 
GCCGACATCC CAGTTTATCG GTTTGTTCAG CGCCCGGGAG ACCTGGTCTG GATCAACGCC 
GGAACTGTGC ATTGGGTGCA GGCTACAGGC TGGTGCAACA ACATCGCGTG GAACGTGGGG 
CCCCTGACAC CCTATCAGTA CCAACTGGCG CTGGAGCGAT TTGAGTGGAA CGAAGTCAAG 
AACGTGAAAT CCATCGTCCC AATGATCCAC GTGTCCTGGA ACGTGGCCCG GACCGTCAAA 
ATCAGTGATC CAGACCTGTA TCGCATGATC AAGTACTGCC TGATGCAGTC TATGAAGCGC 
TGCGTTTTGC AGAGGGACAG CCTGATCCGC GGCGGGAAGA AAATCGCCTA CCAGAGCAGA 
GTGAAAGATG AACCGGCCTA TTACTGCAAC GAGTGTGATG TGGAGGTGTT TAACATCCTA 
TTCGTGACCA GCGAGAGCGG TGGGAAGAAC ACGTACCTGG TGCACTGTGA GAGCTGCTCC 
CGGAGCGCCA GTAGCGCAAG CAGCGCCAGC AGCAACGTCG TCATCTTGGA GCAGTTTAAG 
ACCGAGGAGC TAATGCACAT ATACGACAAC TTCCACCTGG CTCCACCCCC GAACATCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753876.1
CDS	621..5843
Translation	MYRSQDQFGSRFPRDSFISGVNTTWSPPHPRPWAPVSRCSSNMNQPQLPPYLPSGHVTNLGHPSKPYYPISSPPGPRIGKLERVHSCVQALLRDEQQPSLWEQLGQIYESEQEWEDAIHCYQTAARYHGGYELGARISRLQQAHMWNMHATPHHRPKSLPPLQQVWDLMQHEQKRHFGAKRCLPQLKRPVPPSDSSALQLSPHNPHGGEEQPMKRRRSRSPEQQRRGNPGLHSPPASGGSGSLYHQAPVKPSVWNPLTHVPWAERRERQEPRPPHAPSYPPYCPPRPLPPPPPLHQQPRPPSDLSEARGQRCAEAVPPAPPPLHVPYDQRRPRATPPTTPRTDLTTSSDRYQNSAGSPSRICPPLVEQLDPPQTICQPQLPAVSRPPPPLQQGWLPPPAKEEMKKGDPLEDILYGDGPTKVKQEGFYPSRDTQREGNVYNHQPQGITTSKARSEAYQPGSLDHVLQKMDESGLIAPKKEEEIKQEVRNHPLTPELEVGGKQSSHGLSRAEGSYSGGKGSPEGADETSFCPSLAPHHKDTSGSLPVPGSVALPAKPMPNLTRRPLSPNSAFSERTSVCGQEKEQPTGCNQLFEFPGGQLHEPFEEAAEFAKILPDGLDDIMKMLDESIEKEEEGVGNASGMDDSSSTTSSSPPAETMPASSLVSSNQTTVVKSPPPNAKTAEVRPSDTFPYHGPKKAMLEQEASKEAKTAQHRGGSVLKSLANVLEGQKYCYRGSSGAKSAGNPAYFTGAMVSKRNVDPYATQSTSHRAWDTKPGTHVGAAPTSEALKPGVIEKRPEVRTETDILEDIARACETLVEGAKGSAASRTGNERAVEKEPKKSEGIVDRKPDLQWGIEKRDIHWGANKLEGPVKQKVDFKWRSDTQKEVVEKKPEAPRATGQQDYVEKKHGADKPRVPVDRKPETLQKADVQEGVAEIKQETSYGTIRHEVAERLEAALERESKKSVKGAEKKCLPGPADSPSGTAREDPSKIHGANVKEAVKEATAKRLQGVNDRESPKKNTDSLAQGGVSKNVAAEVAKPNADSKDTKSSEEAPMPTMEAGDNGTKGWEAGKPIERLTISLPAPARSKEESRRRERKHRKSRRDCSKKSTDRRSLKDKERQVLGNLDLQSQCRDTSNSTLKQSSRKSETGRRGCEEAHHHQPTPPTPPTSAPPPTSHGTANSDLLKMRPFTEGPPKELKIRLIKVESGDRETFIASEVEERRLQASELTINNTAAEIVRATRSMVLKGKFKESYLLPSMSVKPPLGTEQNLPREKLNPPTPSIYLESKRDAFSPILLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKTLVDANGEHMVEVRTQVQQPSDENWDPSGTRQIWHCESSRSHTTIAKYAQYQASSFQESLQEDKESDDDSNEPDSTTETPPPSSTNPEHKTPQIIKFGTNIDLSDPKRWKPQLQELQKLPSFMRVTSNANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHENYWEKISAFCDKHNLDYLTGSWWPVLEDLYRADIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTPYQYQLALERFEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLYRMIKYCLMQSMKRCVLQRDSLIRGGKKIAYQSRVKDEPAYYCNECDVEVFNILFVTSESGGKNTYLVHCESCSRSASSASSASSNVVILEQFKTEELMHIYDNFHLAPPPNIR

Target ORF information:

RefSeq Version	XM_031898016.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898016.1

ORF Insert Sequence:

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT 
GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG 
CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA 
CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT 
CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG 
CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC 
AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA 
ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA 
CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA 
GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG 
GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC 
CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC 
CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA 
GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA 
CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA 
GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA 
GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT 
CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG 
AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG 
CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG 
AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG 
GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC 
CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG 
GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA 
GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC 
GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA 
GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT 
ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC 
CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG 
ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG 
TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA 
GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG 
GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT 
ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC 
TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG 
ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT 
GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG 
AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT 
ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG 
GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT 
TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC 
GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC 
AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT 
GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG 
CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC 
CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG 
AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT 
ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT 
GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC 
ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG 
TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG 
AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG 
CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT 
ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC 
CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA 
CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT 
CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC 
TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG 
TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT 
ACCACAGAAA CCCCACCCCC CAGTAGCACA AACCCGGAGC ACAAGACCCC ACAGATTATC 
AAGTTCGGCA CCAATATTGA TCTGTCTGAT CCCAAAAGGT GGAAACCTCA GTTACAGGAG 
CTGCAGAAGT TGCCATCTTT CATGCGTGTT ACATCCAATG CCAACATGCT AAGCCACGTG 
GGCCATACTA TACTTGGCAT GAACACCGTT CAGCTCTACA TGAAGGTTCC GGGCAGCAGG 
ACCCCGGGTC ACCAGGAGAA TAACAACTTC TGCTCTGTGA ATATAAACAT CGGCCCCGGG 
GACTGCGAAT GGTTCGCTGT ACATGAAAAT TACTGGGAGA AAATCAGCGC CTTCTGTGAC 
AAACACAATC TGGACTACCT GACCGGATCT TGGTGGCCGG TTCTAGAAGA CCTGTACCGC 
GCCGACATCC CAGTTTATCG GTTTGTTCAG CGCCCGGGAG ACCTGGTCTG GATCAACGCC 
GGAACTGTGC ATTGGGTGCA GGCTACAGGC TGGTGCAACA ACATCGCGTG GAACGTGGGG 
CCCCTGACAC CCTATCAGTA CCAACTGGCG CTGGAGCGAT TTGAGTGGAA CGAAGTCAAG 
AACGTGAAAT CCATCGTCCC AATGATCCAC GTGTCCTGGA ACGTGGCCCG GACCGTCAAA 
ATCAGTGATC CAGACCTGTA TCGCATGATC AAGTACTGCC TGATGCAGTC TATGAAGCGC 
TGCGTTTTGC AGAGGGACAG CCTGATCCGC GGCGGGAAGA AAATCGCCTA CCAGAGCAGA 
GTGAAAGATG AACCGGCCTA TTACTGCAAC GAGTGTGATG TGGAGGTGTT TAACATCCTA 
TTCGTGACCA GCGAGAGCGG TGGGAAGAAC ACGTACCTGG TGCACTGTGA GAGCTGCTCC 
CGGAGCGCCA GTAGCGCAAG CAGCGCCAGC AGCAACGTCG TCATCTTGGA GCAGTTTAAG 
ACCGAGGAGC TAATGCACAT ATACGACAAC TTCCACCTGG CTCCACCCCC GAACATCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6b ( XM_031898017.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753877.1 Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa53612
Clone ID Related Accession (Same CDS sequence)	XM_031898015.1 , XM_031898016.1 , XM_031898017.1
Accession Version	XM_031898017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT 
GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG 
CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA 
CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT 
CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG 
CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC 
AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA 
ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA 
CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA 
GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG 
GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC 
CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC 
CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA 
GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA 
CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA 
GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA 
GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT 
CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG 
AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG 
CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG 
AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG 
GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC 
CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG 
GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA 
GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC 
GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA 
GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT 
ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC 
CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG 
ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG 
TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA 
GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG 
GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT 
ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC 
TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG 
ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT 
GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG 
AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT 
ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG 
GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT 
TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC 
GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC 
AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT 
GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG 
CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC 
CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG 
AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT 
ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT 
GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC 
ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG 
TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG 
AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG 
CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT 
ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC 
CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA 
CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT 
CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC 
TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG 
TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT 
ACCACAGAAA CCCCACCCCC CAGTAGCACA AACCCGGAGC ACAAGACCCC ACAGATTATC 
AAGTTCGGCA CCAATATTGA TCTGTCTGAT CCCAAAAGGT GGAAACCTCA GTTACAGGAG 
CTGCAGAAGT TGCCATCTTT CATGCGTGTT ACATCCAATG CCAACATGCT AAGCCACGTG 
GGCCATACTA TACTTGGCAT GAACACCGTT CAGCTCTACA TGAAGGTTCC GGGCAGCAGG 
ACCCCGGGTC ACCAGGAGAA TAACAACTTC TGCTCTGTGA ATATAAACAT CGGCCCCGGG 
GACTGCGAAT GGTTCGCTGT ACATGAAAAT TACTGGGAGA AAATCAGCGC CTTCTGTGAC 
AAACACAATC TGGACTACCT GACCGGATCT TGGTGGCCGG TTCTAGAAGA CCTGTACCGC 
GCCGACATCC CAGTTTATCG GTTTGTTCAG CGCCCGGGAG ACCTGGTCTG GATCAACGCC 
GGAACTGTGC ATTGGGTGCA GGCTACAGGC TGGTGCAACA ACATCGCGTG GAACGTGGGG 
CCCCTGACAC CCTATCAGTA CCAACTGGCG CTGGAGCGAT TTGAGTGGAA CGAAGTCAAG 
AACGTGAAAT CCATCGTCCC AATGATCCAC GTGTCCTGGA ACGTGGCCCG GACCGTCAAA 
ATCAGTGATC CAGACCTGTA TCGCATGATC AAGTACTGCC TGATGCAGTC TATGAAGCGC 
TGCGTTTTGC AGAGGGACAG CCTGATCCGC GGCGGGAAGA AAATCGCCTA CCAGAGCAGA 
GTGAAAGATG AACCGGCCTA TTACTGCAAC GAGTGTGATG TGGAGGTGTT TAACATCCTA 
TTCGTGACCA GCGAGAGCGG TGGGAAGAAC ACGTACCTGG TGCACTGTGA GAGCTGCTCC 
CGGAGCGCCA GTAGCGCAAG CAGCGCCAGC AGCAACGTCG TCATCTTGGA GCAGTTTAAG 
ACCGAGGAGC TAATGCACAT ATACGACAAC TTCCACCTGG CTCCACCCCC GAACATCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753877.1
CDS	904..6126
Translation	MYRSQDQFGSRFPRDSFISGVNTTWSPPHPRPWAPVSRCSSNMNQPQLPPYLPSGHVTNLGHPSKPYYPISSPPGPRIGKLERVHSCVQALLRDEQQPSLWEQLGQIYESEQEWEDAIHCYQTAARYHGGYELGARISRLQQAHMWNMHATPHHRPKSLPPLQQVWDLMQHEQKRHFGAKRCLPQLKRPVPPSDSSALQLSPHNPHGGEEQPMKRRRSRSPEQQRRGNPGLHSPPASGGSGSLYHQAPVKPSVWNPLTHVPWAERRERQEPRPPHAPSYPPYCPPRPLPPPPPLHQQPRPPSDLSEARGQRCAEAVPPAPPPLHVPYDQRRPRATPPTTPRTDLTTSSDRYQNSAGSPSRICPPLVEQLDPPQTICQPQLPAVSRPPPPLQQGWLPPPAKEEMKKGDPLEDILYGDGPTKVKQEGFYPSRDTQREGNVYNHQPQGITTSKARSEAYQPGSLDHVLQKMDESGLIAPKKEEEIKQEVRNHPLTPELEVGGKQSSHGLSRAEGSYSGGKGSPEGADETSFCPSLAPHHKDTSGSLPVPGSVALPAKPMPNLTRRPLSPNSAFSERTSVCGQEKEQPTGCNQLFEFPGGQLHEPFEEAAEFAKILPDGLDDIMKMLDESIEKEEEGVGNASGMDDSSSTTSSSPPAETMPASSLVSSNQTTVVKSPPPNAKTAEVRPSDTFPYHGPKKAMLEQEASKEAKTAQHRGGSVLKSLANVLEGQKYCYRGSSGAKSAGNPAYFTGAMVSKRNVDPYATQSTSHRAWDTKPGTHVGAAPTSEALKPGVIEKRPEVRTETDILEDIARACETLVEGAKGSAASRTGNERAVEKEPKKSEGIVDRKPDLQWGIEKRDIHWGANKLEGPVKQKVDFKWRSDTQKEVVEKKPEAPRATGQQDYVEKKHGADKPRVPVDRKPETLQKADVQEGVAEIKQETSYGTIRHEVAERLEAALERESKKSVKGAEKKCLPGPADSPSGTAREDPSKIHGANVKEAVKEATAKRLQGVNDRESPKKNTDSLAQGGVSKNVAAEVAKPNADSKDTKSSEEAPMPTMEAGDNGTKGWEAGKPIERLTISLPAPARSKEESRRRERKHRKSRRDCSKKSTDRRSLKDKERQVLGNLDLQSQCRDTSNSTLKQSSRKSETGRRGCEEAHHHQPTPPTPPTSAPPPTSHGTANSDLLKMRPFTEGPPKELKIRLIKVESGDRETFIASEVEERRLQASELTINNTAAEIVRATRSMVLKGKFKESYLLPSMSVKPPLGTEQNLPREKLNPPTPSIYLESKRDAFSPILLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKTLVDANGEHMVEVRTQVQQPSDENWDPSGTRQIWHCESSRSHTTIAKYAQYQASSFQESLQEDKESDDDSNEPDSTTETPPPSSTNPEHKTPQIIKFGTNIDLSDPKRWKPQLQELQKLPSFMRVTSNANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHENYWEKISAFCDKHNLDYLTGSWWPVLEDLYRADIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTPYQYQLALERFEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLYRMIKYCLMQSMKRCVLQRDSLIRGGKKIAYQSRVKDEPAYYCNECDVEVFNILFVTSESGGKNTYLVHCESCSRSASSASSASSNVVILEQFKTEELMHIYDNFHLAPPPNIR

Target ORF information:

RefSeq Version	XM_031898017.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898017.1

ORF Insert Sequence:

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT 
GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG 
CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA 
CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT 
CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG 
CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC 
AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA 
ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA 
CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA 
GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG 
GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC 
CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC 
CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA 
GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA 
CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA 
GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA 
GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT 
CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG 
AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG 
CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG 
AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG 
GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC 
CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG 
GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA 
GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC 
GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA 
GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT 
ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC 
CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG 
ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG 
TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA 
GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG 
GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT 
ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC 
TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG 
ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT 
GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG 
AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT 
ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG 
GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT 
TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC 
GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC 
AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT 
GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG 
CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC 
CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG 
AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT 
ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT 
GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC 
ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG 
TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG 
AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG 
CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT 
ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC 
CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA 
CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT 
CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC 
TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG 
TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT 
ACCACAGAAA CCCCACCCCC CAGTAGCACA AACCCGGAGC ACAAGACCCC ACAGATTATC 
AAGTTCGGCA CCAATATTGA TCTGTCTGAT CCCAAAAGGT GGAAACCTCA GTTACAGGAG 
CTGCAGAAGT TGCCATCTTT CATGCGTGTT ACATCCAATG CCAACATGCT AAGCCACGTG 
GGCCATACTA TACTTGGCAT GAACACCGTT CAGCTCTACA TGAAGGTTCC GGGCAGCAGG 
ACCCCGGGTC ACCAGGAGAA TAACAACTTC TGCTCTGTGA ATATAAACAT CGGCCCCGGG 
GACTGCGAAT GGTTCGCTGT ACATGAAAAT TACTGGGAGA AAATCAGCGC CTTCTGTGAC 
AAACACAATC TGGACTACCT GACCGGATCT TGGTGGCCGG TTCTAGAAGA CCTGTACCGC 
GCCGACATCC CAGTTTATCG GTTTGTTCAG CGCCCGGGAG ACCTGGTCTG GATCAACGCC 
GGAACTGTGC ATTGGGTGCA GGCTACAGGC TGGTGCAACA ACATCGCGTG GAACGTGGGG 
CCCCTGACAC CCTATCAGTA CCAACTGGCG CTGGAGCGAT TTGAGTGGAA CGAAGTCAAG 
AACGTGAAAT CCATCGTCCC AATGATCCAC GTGTCCTGGA ACGTGGCCCG GACCGTCAAA 
ATCAGTGATC CAGACCTGTA TCGCATGATC AAGTACTGCC TGATGCAGTC TATGAAGCGC 
TGCGTTTTGC AGAGGGACAG CCTGATCCGC GGCGGGAAGA AAATCGCCTA CCAGAGCAGA 
GTGAAAGATG AACCGGCCTA TTACTGCAAC GAGTGTGATG TGGAGGTGTT TAACATCCTA 
TTCGTGACCA GCGAGAGCGG TGGGAAGAAC ACGTACCTGG TGCACTGTGA GAGCTGCTCC 
CGGAGCGCCA GTAGCGCAAG CAGCGCCAGC AGCAACGTCG TCATCTTGGA GCAGTTTAAG 
ACCGAGGAGC TAATGCACAT ATACGACAAC TTCCACCTGG CTCCACCCCC GAACATCCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6b ( XM_031898018.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753878.1 Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X4, mRNA.

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CloneID	OXa53613
Clone ID Related Accession (Same CDS sequence)	XM_031898018.1
Accession Version	XM_031898018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGAAGA GACATTTCGG AGCTAAACGG 
TGTCTCCCGC AGCTGAAGCG CCCGGTGCCC CCCTCAGACT CTTCCGCACT CCAGCTGAGC 
CCACACAATC CCCATGGAGG GGAGGAGCAA CCGATGAAAC GGAGGAGGAG CCGGAGCCCC 
GAACAGCAAA GGAGGGGGAA CCCTGGCCTG CACTCTCCTC CAGCCTCAGG GGGTTCTGGG 
AGTTTGTACC ATCAGGCACC CGTCAAGCCC AGTGTTTGGA ATCCACTCAC CCATGTGCCC 
TGGGCTGAGC GCAGGGAGCG GCAGGAACCC AGACCCCCCC ACGCGCCGTC TTACCCACCA 
TATTGCCCTC CACGCCCTCT ACCGCCTCCA CCACCACTTC ACCAGCAGCC CCGCCCTCCA 
AGTGACCTTA GTGAGGCTCG GGGGCAGAGG TGTGCAGAGG CCGTCCCCCC AGCACCGCCC 
CCTCTCCACG TGCCTTACGA TCAGAGAAGA CCCCGCGCCA CCCCGCCGAC TACCCCCAGG 
ACCGATCTCA CCACTTCCTC TGATCGCTAC CAGAATTCCG CAGGCAGCCC GTCTAGAATC 
TGCCCTCCTC TTGTAGAGCA GCTGGACCCC CCACAAACTA TATGTCAACC CCAGCTACCA 
GCTGTCTCTC GACCTCCTCC TCCTCTGCAA CAGGGATGGC TGCCCCCCCC AGCCAAAGAG 
GAGATGAAGA AGGGAGACCC ACTAGAGGAC ATTCTGTATG GAGATGGACC TACAAAGGTT 
AAACAGGAAG GGTTTTACCC ATCCCGTGAC ACCCAAAGAG AGGGCAATGT CTACAACCAC 
CAACCTCAGG GCATTACCAC CAGCAAAGCC AGGTCTGAGG CCTACCAGCC TGGAAGCCTG 
GATCACGTAC TGCAGAAGAT GGATGAATCT GGGTTAATTG CCCCAAAGAA GGAGGAAGAG 
ATTAAACAGG AAGTTAGAAA TCATCCATTG ACCCCAGAAC TGGAGGTTGG AGGCAAACAA 
TCAAGCCATG GACTCTCTAG AGCTGAGGGC AGCTATTCTG GTGGGAAGGG GTCACCAGAA 
GGCGCGGATG AAACGTCTTT CTGCCCCTCT TTGGCCCCCC ACCACAAAGA TACCTCAGGG 
AGCCTCCCGG TTCCAGGCTC AGTTGCATTA CCAGCCAAGC CAATGCCTAA CTTGACTCGC 
AGGCCACTAA GTCCGAACTC GGCCTTCTCG GAGAGAACGT CGGTGTGCGG ACAGGAGAAG 
GAGCAACCCA CTGGATGCAA CCAGCTCTTT GAATTCCCCG GGGGGCAGTT GCACGAGCCC 
TTTGAGGAAG CCGCGGAGTT TGCAAAAATA TTACCGGACG GGCTTGACGA CATCATGAAA 
ATGCTTGACG AGTCGATAGA AAAGGAAGAG GAAGGGGTTG GGAACGCCAG TGGGATGGAC 
GATAGTTCCT CCACTACATC CAGCAGTCCA CCAGCAGAGA CCATGCCAGC ATCCAGCCTA 
GTGAGCTCAA ATCAGACCAC TGTTGTGAAA AGCCCACCGC CCAATGCCAA AACAGCGGAA 
GTCCGGCCAA GTGACACCTT CCCCTATCAT GGCCCTAAGA AAGCCATGCT GGAGCAAGAA 
GCAAGCAAGG AGGCAAAGAC TGCGCAGCAC AGGGGAGGCA GCGTTCTGAA ATCACTCGCA 
AATGTTCTAG AGGGGCAGAA GTATTGCTAC CGAGGCAGCT CTGGGGCGAA GTCCGCAGGA 
AACCCGGCAT ACTTCACTGG TGCTATGGTG AGCAAGCGTA ATGTTGACCC ATACGCTACG 
CAATCTACCT CACACCGGGC CTGGGACACC AAGCCAGGGA CGCATGTTGG GGCAGCCCCT 
ACCTCAGAGG CTCTTAAGCC AGGGGTGATT GAGAAAAGGC CAGAGGTACG GACAGAGACG 
GACATTCTGG AGGACATAGC GAGGGCTTGT GAGACTTTGG TTGAGGGTGC AAAGGGGTCG 
GCGGCAAGCA GGACGGGAAA CGAGAGAGCA GTTGAGAAAG AGCCCAAAAA GTCTGAAGGG 
ATTGTGGACA GGAAGCCAGA TTTACAGTGG GGCATAGAAA AACGTGACAT CCATTGGGGG 
GCAAATAAAC TAGAAGGACC AGTTAAGCAG AAAGTAGATT TCAAGTGGAG AAGCGATACC 
CAGAAAGAAG TGGTTGAGAA GAAGCCAGAG GCTCCCAGGG CTACTGGCCA ACAGGACTAT 
GTTGAGAAGA AACATGGGGC AGACAAACCC CGCGTCCCCG TTGACAGGAA GCCTGAGACT 
TTGCAGAAGG CAGATGTACA GGAAGGAGTT GCTGAAATAA AGCAGGAAAC ATCTTATGGC 
ACAATCAGGC ATGAAGTGGC AGAGAGGCTG GAGGCGGCTC TTGAGAGAGA ATCCAAGAAG 
TCGGTGAAGG GAGCAGAGAA GAAATGTCTC CCAGGTCCAG CTGACTCCCC CTCAGGTACA 
GCCCGGGAAG ATCCCTCTAA AATACACGGT GCTAATGTCA AGGAGGCTGT AAAGGAGGCG 
ACAGCTAAGC GGCTGCAAGG GGTAAATGAC AGAGAGTCAC CAAAAAAGAA CACCGATTCT 
TTGGCCCAGG GAGGGGTCAG TAAGAACGTG GCGGCTGAAG TGGCAAAACC GAATGCCGAT 
AGCAAGGACA CTAAGTCATC TGAGGAAGCA CCGATGCCAA CAATGGAGGC AGGAGACAAT 
GGCACAAAGG GATGGGAGGC TGGGAAACCC ATTGAGAGGT TGACTATATC TCTACCTGCC 
CCTGCTCGGA GCAAGGAGGA AAGCAGGAGG AGAGAGCGGA AGCACAGGAA GTCCAGGCGG 
GACTGTAGTA AGAAGTCAAC TGACCGCAGG TCTCTGAAGG ACAAGGAGAG GCAAGTCCTA 
GGCAACCTAG ATCTCCAGAG CCAGTGCCGG GACACCTCAA ATTCTACACT TAAGCAGAGT 
TCAAGGAAGT CCGAGACTGG CAGAAGAGGG TGCGAGGAAG CGCATCACCA CCAACCTACC 
CCACCTACTC CTCCAACTTC CGCACCCCCT CCAACGTCCC ACGGCACAGC CAACTCTGAC 
CTTCTAAAGA TGAGACCCTT CACCGAAGGG CCGCCTAAAG AGCTCAAGAT CCGCCTCATA 
AAGGTTGAAA GCGGAGATCG GGAGACTTTT ATAGCCTCCG AGGTGGAAGA GCGAAGGTTG 
CAGGCCAGTG AGCTGACCAT TAATAACACT GCTGCGGAGA TTGTAAGGGC CACCAGGAGT 
ATGGTGCTAA AAGGGAAGTT CAAAGAGTCC TACCTGCTGC CCTCGATGTC CGTGAAGCCG 
CCCTTGGGGA CGGAGCAGAA CCTTCCACGA GAGAAGCTGA ATCCCCCAAC TCCCAGTATA 
TATCTGGAGA GCAAACGAGA TGCCTTCTCC CCTATCCTGC TTCAGTTCTG CACCGACCCC 
AAGAATCCCA TCACTGTTAT CCGCGGTTTG GCCGGGTCCC TGCGTCTCAA CCTCGGACTC 
TTCTCCACCA AAACGCTCGT TGACGCCAAC GGGGAGCACA TGGTGGAGGT GCGCACTCAG 
GTACAACAGC CGTCAGATGA GAACTGGGAC CCGTCGGGCA CTCGCCAGAT CTGGCACTGC 
GAGAGCAGCC GTTCCCACAC CACCATCGCC AAATATGCAC AGTATCAGGC CTCCTCGTTC 
CAGGAGTCCC TGCAGGAGGA TAAGGAAAGT GATGACGACT CCAATGAACC AGATAGTACC 
ACAGAAACCC CACCCCCCAG TAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG 
TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG 
CAGAAGTTGC CATCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC 
CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC 
CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC 
TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA 
CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC 
GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA 
ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC 
CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC 
GTGAAATCCA TCGTCCCAAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC 
AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC 
GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG 
AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC 
GTGACCAGCG AGAGCGGTGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG 
AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTTAAGACC 
GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753878.1
CDS	542..5761
Translation	MYRSQDQFGSRFPRDSFISGVNTTWSPPHPRPWAPVSRCSSNMNQPQLPPYLPSGHVTNLGHPSKPYYPISSPPGPRIGKLERVHSCVQALLRDEQQPSLWEQLGQIYESEQEWEDAIHCYQTAARYHGGYELGARISRLQQAHMWNMHATPHHRPKSLPPLQQVWDLMQHEKRHFGAKRCLPQLKRPVPPSDSSALQLSPHNPHGGEEQPMKRRRSRSPEQQRRGNPGLHSPPASGGSGSLYHQAPVKPSVWNPLTHVPWAERRERQEPRPPHAPSYPPYCPPRPLPPPPPLHQQPRPPSDLSEARGQRCAEAVPPAPPPLHVPYDQRRPRATPPTTPRTDLTTSSDRYQNSAGSPSRICPPLVEQLDPPQTICQPQLPAVSRPPPPLQQGWLPPPAKEEMKKGDPLEDILYGDGPTKVKQEGFYPSRDTQREGNVYNHQPQGITTSKARSEAYQPGSLDHVLQKMDESGLIAPKKEEEIKQEVRNHPLTPELEVGGKQSSHGLSRAEGSYSGGKGSPEGADETSFCPSLAPHHKDTSGSLPVPGSVALPAKPMPNLTRRPLSPNSAFSERTSVCGQEKEQPTGCNQLFEFPGGQLHEPFEEAAEFAKILPDGLDDIMKMLDESIEKEEEGVGNASGMDDSSSTTSSSPPAETMPASSLVSSNQTTVVKSPPPNAKTAEVRPSDTFPYHGPKKAMLEQEASKEAKTAQHRGGSVLKSLANVLEGQKYCYRGSSGAKSAGNPAYFTGAMVSKRNVDPYATQSTSHRAWDTKPGTHVGAAPTSEALKPGVIEKRPEVRTETDILEDIARACETLVEGAKGSAASRTGNERAVEKEPKKSEGIVDRKPDLQWGIEKRDIHWGANKLEGPVKQKVDFKWRSDTQKEVVEKKPEAPRATGQQDYVEKKHGADKPRVPVDRKPETLQKADVQEGVAEIKQETSYGTIRHEVAERLEAALERESKKSVKGAEKKCLPGPADSPSGTAREDPSKIHGANVKEAVKEATAKRLQGVNDRESPKKNTDSLAQGGVSKNVAAEVAKPNADSKDTKSSEEAPMPTMEAGDNGTKGWEAGKPIERLTISLPAPARSKEESRRRERKHRKSRRDCSKKSTDRRSLKDKERQVLGNLDLQSQCRDTSNSTLKQSSRKSETGRRGCEEAHHHQPTPPTPPTSAPPPTSHGTANSDLLKMRPFTEGPPKELKIRLIKVESGDRETFIASEVEERRLQASELTINNTAAEIVRATRSMVLKGKFKESYLLPSMSVKPPLGTEQNLPREKLNPPTPSIYLESKRDAFSPILLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKTLVDANGEHMVEVRTQVQQPSDENWDPSGTRQIWHCESSRSHTTIAKYAQYQASSFQESLQEDKESDDDSNEPDSTTETPPPSSTNPEHKTPQIIKFGTNIDLSDPKRWKPQLQELQKLPSFMRVTSNANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHENYWEKISAFCDKHNLDYLTGSWWPVLEDLYRADIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTPYQYQLALERFEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLYRMIKYCLMQSMKRCVLQRDSLIRGGKKIAYQSRVKDEPAYYCNECDVEVFNILFVTSESGGKNTYLVHCESCSRSASSASSASSNVVILEQFKTEELMHIYDNFHLAPPPNIR

Target ORF information:

RefSeq Version	XM_031898018.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898018.1

ORF Insert Sequence:

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGAAGA GACATTTCGG AGCTAAACGG 
TGTCTCCCGC AGCTGAAGCG CCCGGTGCCC CCCTCAGACT CTTCCGCACT CCAGCTGAGC 
CCACACAATC CCCATGGAGG GGAGGAGCAA CCGATGAAAC GGAGGAGGAG CCGGAGCCCC 
GAACAGCAAA GGAGGGGGAA CCCTGGCCTG CACTCTCCTC CAGCCTCAGG GGGTTCTGGG 
AGTTTGTACC ATCAGGCACC CGTCAAGCCC AGTGTTTGGA ATCCACTCAC CCATGTGCCC 
TGGGCTGAGC GCAGGGAGCG GCAGGAACCC AGACCCCCCC ACGCGCCGTC TTACCCACCA 
TATTGCCCTC CACGCCCTCT ACCGCCTCCA CCACCACTTC ACCAGCAGCC CCGCCCTCCA 
AGTGACCTTA GTGAGGCTCG GGGGCAGAGG TGTGCAGAGG CCGTCCCCCC AGCACCGCCC 
CCTCTCCACG TGCCTTACGA TCAGAGAAGA CCCCGCGCCA CCCCGCCGAC TACCCCCAGG 
ACCGATCTCA CCACTTCCTC TGATCGCTAC CAGAATTCCG CAGGCAGCCC GTCTAGAATC 
TGCCCTCCTC TTGTAGAGCA GCTGGACCCC CCACAAACTA TATGTCAACC CCAGCTACCA 
GCTGTCTCTC GACCTCCTCC TCCTCTGCAA CAGGGATGGC TGCCCCCCCC AGCCAAAGAG 
GAGATGAAGA AGGGAGACCC ACTAGAGGAC ATTCTGTATG GAGATGGACC TACAAAGGTT 
AAACAGGAAG GGTTTTACCC ATCCCGTGAC ACCCAAAGAG AGGGCAATGT CTACAACCAC 
CAACCTCAGG GCATTACCAC CAGCAAAGCC AGGTCTGAGG CCTACCAGCC TGGAAGCCTG 
GATCACGTAC TGCAGAAGAT GGATGAATCT GGGTTAATTG CCCCAAAGAA GGAGGAAGAG 
ATTAAACAGG AAGTTAGAAA TCATCCATTG ACCCCAGAAC TGGAGGTTGG AGGCAAACAA 
TCAAGCCATG GACTCTCTAG AGCTGAGGGC AGCTATTCTG GTGGGAAGGG GTCACCAGAA 
GGCGCGGATG AAACGTCTTT CTGCCCCTCT TTGGCCCCCC ACCACAAAGA TACCTCAGGG 
AGCCTCCCGG TTCCAGGCTC AGTTGCATTA CCAGCCAAGC CAATGCCTAA CTTGACTCGC 
AGGCCACTAA GTCCGAACTC GGCCTTCTCG GAGAGAACGT CGGTGTGCGG ACAGGAGAAG 
GAGCAACCCA CTGGATGCAA CCAGCTCTTT GAATTCCCCG GGGGGCAGTT GCACGAGCCC 
TTTGAGGAAG CCGCGGAGTT TGCAAAAATA TTACCGGACG GGCTTGACGA CATCATGAAA 
ATGCTTGACG AGTCGATAGA AAAGGAAGAG GAAGGGGTTG GGAACGCCAG TGGGATGGAC 
GATAGTTCCT CCACTACATC CAGCAGTCCA CCAGCAGAGA CCATGCCAGC ATCCAGCCTA 
GTGAGCTCAA ATCAGACCAC TGTTGTGAAA AGCCCACCGC CCAATGCCAA AACAGCGGAA 
GTCCGGCCAA GTGACACCTT CCCCTATCAT GGCCCTAAGA AAGCCATGCT GGAGCAAGAA 
GCAAGCAAGG AGGCAAAGAC TGCGCAGCAC AGGGGAGGCA GCGTTCTGAA ATCACTCGCA 
AATGTTCTAG AGGGGCAGAA GTATTGCTAC CGAGGCAGCT CTGGGGCGAA GTCCGCAGGA 
AACCCGGCAT ACTTCACTGG TGCTATGGTG AGCAAGCGTA ATGTTGACCC ATACGCTACG 
CAATCTACCT CACACCGGGC CTGGGACACC AAGCCAGGGA CGCATGTTGG GGCAGCCCCT 
ACCTCAGAGG CTCTTAAGCC AGGGGTGATT GAGAAAAGGC CAGAGGTACG GACAGAGACG 
GACATTCTGG AGGACATAGC GAGGGCTTGT GAGACTTTGG TTGAGGGTGC AAAGGGGTCG 
GCGGCAAGCA GGACGGGAAA CGAGAGAGCA GTTGAGAAAG AGCCCAAAAA GTCTGAAGGG 
ATTGTGGACA GGAAGCCAGA TTTACAGTGG GGCATAGAAA AACGTGACAT CCATTGGGGG 
GCAAATAAAC TAGAAGGACC AGTTAAGCAG AAAGTAGATT TCAAGTGGAG AAGCGATACC 
CAGAAAGAAG TGGTTGAGAA GAAGCCAGAG GCTCCCAGGG CTACTGGCCA ACAGGACTAT 
GTTGAGAAGA AACATGGGGC AGACAAACCC CGCGTCCCCG TTGACAGGAA GCCTGAGACT 
TTGCAGAAGG CAGATGTACA GGAAGGAGTT GCTGAAATAA AGCAGGAAAC ATCTTATGGC 
ACAATCAGGC ATGAAGTGGC AGAGAGGCTG GAGGCGGCTC TTGAGAGAGA ATCCAAGAAG 
TCGGTGAAGG GAGCAGAGAA GAAATGTCTC CCAGGTCCAG CTGACTCCCC CTCAGGTACA 
GCCCGGGAAG ATCCCTCTAA AATACACGGT GCTAATGTCA AGGAGGCTGT AAAGGAGGCG 
ACAGCTAAGC GGCTGCAAGG GGTAAATGAC AGAGAGTCAC CAAAAAAGAA CACCGATTCT 
TTGGCCCAGG GAGGGGTCAG TAAGAACGTG GCGGCTGAAG TGGCAAAACC GAATGCCGAT 
AGCAAGGACA CTAAGTCATC TGAGGAAGCA CCGATGCCAA CAATGGAGGC AGGAGACAAT 
GGCACAAAGG GATGGGAGGC TGGGAAACCC ATTGAGAGGT TGACTATATC TCTACCTGCC 
CCTGCTCGGA GCAAGGAGGA AAGCAGGAGG AGAGAGCGGA AGCACAGGAA GTCCAGGCGG 
GACTGTAGTA AGAAGTCAAC TGACCGCAGG TCTCTGAAGG ACAAGGAGAG GCAAGTCCTA 
GGCAACCTAG ATCTCCAGAG CCAGTGCCGG GACACCTCAA ATTCTACACT TAAGCAGAGT 
TCAAGGAAGT CCGAGACTGG CAGAAGAGGG TGCGAGGAAG CGCATCACCA CCAACCTACC 
CCACCTACTC CTCCAACTTC CGCACCCCCT CCAACGTCCC ACGGCACAGC CAACTCTGAC 
CTTCTAAAGA TGAGACCCTT CACCGAAGGG CCGCCTAAAG AGCTCAAGAT CCGCCTCATA 
AAGGTTGAAA GCGGAGATCG GGAGACTTTT ATAGCCTCCG AGGTGGAAGA GCGAAGGTTG 
CAGGCCAGTG AGCTGACCAT TAATAACACT GCTGCGGAGA TTGTAAGGGC CACCAGGAGT 
ATGGTGCTAA AAGGGAAGTT CAAAGAGTCC TACCTGCTGC CCTCGATGTC CGTGAAGCCG 
CCCTTGGGGA CGGAGCAGAA CCTTCCACGA GAGAAGCTGA ATCCCCCAAC TCCCAGTATA 
TATCTGGAGA GCAAACGAGA TGCCTTCTCC CCTATCCTGC TTCAGTTCTG CACCGACCCC 
AAGAATCCCA TCACTGTTAT CCGCGGTTTG GCCGGGTCCC TGCGTCTCAA CCTCGGACTC 
TTCTCCACCA AAACGCTCGT TGACGCCAAC GGGGAGCACA TGGTGGAGGT GCGCACTCAG 
GTACAACAGC CGTCAGATGA GAACTGGGAC CCGTCGGGCA CTCGCCAGAT CTGGCACTGC 
GAGAGCAGCC GTTCCCACAC CACCATCGCC AAATATGCAC AGTATCAGGC CTCCTCGTTC 
CAGGAGTCCC TGCAGGAGGA TAAGGAAAGT GATGACGACT CCAATGAACC AGATAGTACC 
ACAGAAACCC CACCCCCCAG TAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG 
TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG 
CAGAAGTTGC CATCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC 
CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC 
CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC 
TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA 
CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC 
GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA 
ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC 
CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC 
GTGAAATCCA TCGTCCCAAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC 
AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC 
GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG 
AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC 
GTGACCAGCG AGAGCGGTGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG 
AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTTAAGACC 
GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6b ( XM_031898019.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753879.1 Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X5, mRNA.

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CloneID	OXa53614
Clone ID Related Accession (Same CDS sequence)	XM_031898019.1 , XM_031898020.1
Accession Version	XM_031898019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT 
GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG 
CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA 
CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT 
CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG 
CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC 
AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA 
ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA 
CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA 
GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG 
GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC 
CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC 
CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA 
GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA 
CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA 
GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA 
GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT 
CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG 
AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG 
CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG 
AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG 
GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC 
CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG 
GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA 
GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC 
GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA 
GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT 
ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC 
CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG 
ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG 
TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA 
GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG 
GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT 
ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC 
TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG 
ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT 
GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG 
AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT 
ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG 
GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT 
TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC 
GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC 
AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT 
GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG 
CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC 
CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG 
AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT 
ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT 
GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC 
ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG 
TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG 
AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG 
CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT 
ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC 
CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA 
CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT 
CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC 
TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG 
TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT 
ACCACAGAAA CCCCACCCCC CAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG 
TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG 
CAGAAGTTGC CATCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC 
CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC 
CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC 
TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA 
CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC 
GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA 
ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC 
CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC 
GTGAAATCCA TCGTCCCAAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC 
AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC 
GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG 
AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC 
GTGACCAGCG AGAGCGGTGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG 
AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTTAAGACC 
GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753879.1
CDS	542..5761
Translation	MYRSQDQFGSRFPRDSFISGVNTTWSPPHPRPWAPVSRCSSNMNQPQLPPYLPSGHVTNLGHPSKPYYPISSPPGPRIGKLERVHSCVQALLRDEQQPSLWEQLGQIYESEQEWEDAIHCYQTAARYHGGYELGARISRLQQAHMWNMHATPHHRPKSLPPLQQVWDLMQHEQKRHFGAKRCLPQLKRPVPPSDSSALQLSPHNPHGGEEQPMKRRRSRSPEQQRRGNPGLHSPPASGGSGSLYHQAPVKPSVWNPLTHVPWAERRERQEPRPPHAPSYPPYCPPRPLPPPPPLHQQPRPPSDLSEARGQRCAEAVPPAPPPLHVPYDQRRPRATPPTTPRTDLTTSSDRYQNSAGSPSRICPPLVEQLDPPQTICQPQLPAVSRPPPPLQQGWLPPPAKEEMKKGDPLEDILYGDGPTKVKQEGFYPSRDTQREGNVYNHQPQGITTSKARSEAYQPGSLDHVLQKMDESGLIAPKKEEEIKQEVRNHPLTPELEVGGKQSSHGLSRAEGSYSGGKGSPEGADETSFCPSLAPHHKDTSGSLPVPGSVALPAKPMPNLTRRPLSPNSAFSERTSVCGQEKEQPTGCNQLFEFPGGQLHEPFEEAAEFAKILPDGLDDIMKMLDESIEKEEEGVGNASGMDDSSSTTSSSPPAETMPASSLVSSNQTTVVKSPPPNAKTAEVRPSDTFPYHGPKKAMLEQEASKEAKTAQHRGGSVLKSLANVLEGQKYCYRGSSGAKSAGNPAYFTGAMVSKRNVDPYATQSTSHRAWDTKPGTHVGAAPTSEALKPGVIEKRPEVRTETDILEDIARACETLVEGAKGSAASRTGNERAVEKEPKKSEGIVDRKPDLQWGIEKRDIHWGANKLEGPVKQKVDFKWRSDTQKEVVEKKPEAPRATGQQDYVEKKHGADKPRVPVDRKPETLQKADVQEGVAEIKQETSYGTIRHEVAERLEAALERESKKSVKGAEKKCLPGPADSPSGTAREDPSKIHGANVKEAVKEATAKRLQGVNDRESPKKNTDSLAQGGVSKNVAAEVAKPNADSKDTKSSEEAPMPTMEAGDNGTKGWEAGKPIERLTISLPAPARSKEESRRRERKHRKSRRDCSKKSTDRRSLKDKERQVLGNLDLQSQCRDTSNSTLKQSSRKSETGRRGCEEAHHHQPTPPTPPTSAPPPTSHGTANSDLLKMRPFTEGPPKELKIRLIKVESGDRETFIASEVEERRLQASELTINNTAAEIVRATRSMVLKGKFKESYLLPSMSVKPPLGTEQNLPREKLNPPTPSIYLESKRDAFSPILLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKTLVDANGEHMVEVRTQVQQPSDENWDPSGTRQIWHCESSRSHTTIAKYAQYQASSFQESLQEDKESDDDSNEPDSTTETPPPSTNPEHKTPQIIKFGTNIDLSDPKRWKPQLQELQKLPSFMRVTSNANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHENYWEKISAFCDKHNLDYLTGSWWPVLEDLYRADIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTPYQYQLALERFEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLYRMIKYCLMQSMKRCVLQRDSLIRGGKKIAYQSRVKDEPAYYCNECDVEVFNILFVTSESGGKNTYLVHCESCSRSASSASSASSNVVILEQFKTEELMHIYDNFHLAPPPNIR

Target ORF information:

RefSeq Version	XM_031898019.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898019.1

ORF Insert Sequence:

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT 
GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG 
CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA 
CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT 
CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG 
CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC 
AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA 
ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA 
CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA 
GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG 
GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC 
CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC 
CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA 
GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA 
CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA 
GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA 
GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT 
CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG 
AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG 
CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG 
AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG 
GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC 
CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG 
GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA 
GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC 
GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA 
GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT 
ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC 
CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG 
ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG 
TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA 
GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG 
GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT 
ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC 
TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG 
ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT 
GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG 
AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT 
ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG 
GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT 
TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC 
GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC 
AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT 
GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG 
CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC 
CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG 
AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT 
ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT 
GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC 
ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG 
TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG 
AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG 
CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT 
ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC 
CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA 
CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT 
CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC 
TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG 
TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT 
ACCACAGAAA CCCCACCCCC CAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG 
TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG 
CAGAAGTTGC CATCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC 
CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC 
CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC 
TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA 
CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC 
GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA 
ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC 
CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC 
GTGAAATCCA TCGTCCCAAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC 
AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC 
GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG 
AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC 
GTGACCAGCG AGAGCGGTGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG 
AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTTAAGACC 
GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6b ( XM_031898020.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753880.1 Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X6, mRNA.

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CloneID	OXa53614
Clone ID Related Accession (Same CDS sequence)	XM_031898019.1 , XM_031898020.1
Accession Version	XM_031898020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT 
GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG 
CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA 
CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT 
CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG 
CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC 
AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA 
ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA 
CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA 
GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG 
GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC 
CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC 
CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA 
GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA 
CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA 
GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA 
GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT 
CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG 
AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG 
CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG 
AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG 
GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC 
CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG 
GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA 
GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC 
GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA 
GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT 
ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC 
CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG 
ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG 
TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA 
GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG 
GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT 
ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC 
TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG 
ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT 
GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG 
AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT 
ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG 
GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT 
TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC 
GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC 
AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT 
GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG 
CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC 
CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG 
AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT 
ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT 
GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC 
ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG 
TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG 
AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG 
CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT 
ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC 
CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA 
CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT 
CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC 
TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG 
TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT 
ACCACAGAAA CCCCACCCCC CAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG 
TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG 
CAGAAGTTGC CATCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC 
CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC 
CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC 
TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA 
CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC 
GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA 
ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC 
CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC 
GTGAAATCCA TCGTCCCAAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC 
AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC 
GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG 
AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC 
GTGACCAGCG AGAGCGGTGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG 
AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTTAAGACC 
GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753880.1
CDS	621..5840
Translation	MYRSQDQFGSRFPRDSFISGVNTTWSPPHPRPWAPVSRCSSNMNQPQLPPYLPSGHVTNLGHPSKPYYPISSPPGPRIGKLERVHSCVQALLRDEQQPSLWEQLGQIYESEQEWEDAIHCYQTAARYHGGYELGARISRLQQAHMWNMHATPHHRPKSLPPLQQVWDLMQHEQKRHFGAKRCLPQLKRPVPPSDSSALQLSPHNPHGGEEQPMKRRRSRSPEQQRRGNPGLHSPPASGGSGSLYHQAPVKPSVWNPLTHVPWAERRERQEPRPPHAPSYPPYCPPRPLPPPPPLHQQPRPPSDLSEARGQRCAEAVPPAPPPLHVPYDQRRPRATPPTTPRTDLTTSSDRYQNSAGSPSRICPPLVEQLDPPQTICQPQLPAVSRPPPPLQQGWLPPPAKEEMKKGDPLEDILYGDGPTKVKQEGFYPSRDTQREGNVYNHQPQGITTSKARSEAYQPGSLDHVLQKMDESGLIAPKKEEEIKQEVRNHPLTPELEVGGKQSSHGLSRAEGSYSGGKGSPEGADETSFCPSLAPHHKDTSGSLPVPGSVALPAKPMPNLTRRPLSPNSAFSERTSVCGQEKEQPTGCNQLFEFPGGQLHEPFEEAAEFAKILPDGLDDIMKMLDESIEKEEEGVGNASGMDDSSSTTSSSPPAETMPASSLVSSNQTTVVKSPPPNAKTAEVRPSDTFPYHGPKKAMLEQEASKEAKTAQHRGGSVLKSLANVLEGQKYCYRGSSGAKSAGNPAYFTGAMVSKRNVDPYATQSTSHRAWDTKPGTHVGAAPTSEALKPGVIEKRPEVRTETDILEDIARACETLVEGAKGSAASRTGNERAVEKEPKKSEGIVDRKPDLQWGIEKRDIHWGANKLEGPVKQKVDFKWRSDTQKEVVEKKPEAPRATGQQDYVEKKHGADKPRVPVDRKPETLQKADVQEGVAEIKQETSYGTIRHEVAERLEAALERESKKSVKGAEKKCLPGPADSPSGTAREDPSKIHGANVKEAVKEATAKRLQGVNDRESPKKNTDSLAQGGVSKNVAAEVAKPNADSKDTKSSEEAPMPTMEAGDNGTKGWEAGKPIERLTISLPAPARSKEESRRRERKHRKSRRDCSKKSTDRRSLKDKERQVLGNLDLQSQCRDTSNSTLKQSSRKSETGRRGCEEAHHHQPTPPTPPTSAPPPTSHGTANSDLLKMRPFTEGPPKELKIRLIKVESGDRETFIASEVEERRLQASELTINNTAAEIVRATRSMVLKGKFKESYLLPSMSVKPPLGTEQNLPREKLNPPTPSIYLESKRDAFSPILLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKTLVDANGEHMVEVRTQVQQPSDENWDPSGTRQIWHCESSRSHTTIAKYAQYQASSFQESLQEDKESDDDSNEPDSTTETPPPSTNPEHKTPQIIKFGTNIDLSDPKRWKPQLQELQKLPSFMRVTSNANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHENYWEKISAFCDKHNLDYLTGSWWPVLEDLYRADIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTPYQYQLALERFEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLYRMIKYCLMQSMKRCVLQRDSLIRGGKKIAYQSRVKDEPAYYCNECDVEVFNILFVTSESGGKNTYLVHCESCSRSASSASSASSNVVILEQFKTEELMHIYDNFHLAPPPNIR

Target ORF information:

RefSeq Version	XM_031898020.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898020.1

ORF Insert Sequence:

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT 
GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG 
CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA 
CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT 
CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG 
CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC 
AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA 
ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA 
CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA 
GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG 
GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC 
CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC 
CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA 
GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA 
CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA 
GAAGGCGCGG ATGAAACGTC TTTCTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA 
GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT 
CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG 
AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG 
CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG 
AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGATG 
GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC 
CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCAC CGCCCAATGC CAAAACAGCG 
GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA 
GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC 
GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA 
GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT 
ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC 
CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG 
ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG 
TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA 
GGGATTGTGG ACAGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG 
GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT 
ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC 
TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGCGTCC CCGTTGACAG GAAGCCTGAG 
ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT 
GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG 
AAGTCGGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT 
ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG 
GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT 
TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC 
GATAGCAAGG ACACTAAGTC ATCTGAGGAA GCACCGATGC CAACAATGGA GGCAGGAGAC 
AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT 
GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG 
CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC 
CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG 
AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT 
ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT 
GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC 
ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG 
TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG 
AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG 
CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT 
ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC 
CCCAAGAATC CCATCACTGT TATCCGCGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA 
CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT 
CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC 
TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG 
TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT 
ACCACAGAAA CCCCACCCCC CAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG 
TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG 
CAGAAGTTGC CATCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC 
CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC 
CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC 
TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA 
CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC 
GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA 
ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC 
CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC 
GTGAAATCCA TCGTCCCAAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC 
AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC 
GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG 
AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC 
GTGACCAGCG AGAGCGGTGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG 
AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTTAAGACC 
GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6b ( XM_031898021.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753881.1 Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X7, mRNA.

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CloneID	OXa53615
Clone ID Related Accession (Same CDS sequence)	XM_031898021.1
Accession Version	XM_031898021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGG AACCCAGACC CCCCCACGCG CCGTCTTACC CACCATATTG CCCTCCACGC 
CCTCTACCGC CTCCACCACC ACTTCACCAG CAGCCCCGCC CTCCAAGTGA CCTTAGTGAG 
GCTCGGGGGC AGAGGTGTGC AGAGGCCGTC CCCCCAGCAC CGCCCCCTCT CCACGTGCCT 
TACGATCAGA GAAGACCCCG CGCCACCCCG CCGACTACCC CCAGGACCGA TCTCACCACT 
TCCTCTGATC GCTACCAGAA TTCCGCAGGC AGCCCGTCTA GAATCTGCCC TCCTCTTGTA 
GAGCAGCTGG ACCCCCCACA AACTATATGT CAACCCCAGC TACCAGCTGT CTCTCGACCT 
CCTCCTCCTC TGCAACAGGG ATGGCTGCCC CCCCCAGCCA AAGAGGAGAT GAAGAAGGGA 
GACCCACTAG AGGACATTCT GTATGGAGAT GGACCTACAA AGGTTAAACA GGAAGGGTTT 
TACCCATCCC GTGACACCCA AAGAGAGGGC AATGTCTACA ACCACCAACC TCAGGGCATT 
ACCACCAGCA AAGCCAGGTC TGAGGCCTAC CAGCCTGGAA GCCTGGATCA CGTACTGCAG 
AAGATGGATG AATCTGGGTT AATTGCCCCA AAGAAGGAGG AAGAGATTAA ACAGGAAGTT 
AGAAATCATC CATTGACCCC AGAACTGGAG GTTGGAGGCA AACAATCAAG CCATGGACTC 
TCTAGAGCTG AGGGCAGCTA TTCTGGTGGG AAGGGGTCAC CAGAAGGCGC GGATGAAACG 
TCTTTCTGCC CCTCTTTGGC CCCCCACCAC AAAGATACCT CAGGGAGCCT CCCGGTTCCA 
GGCTCAGTTG CATTACCAGC CAAGCCAATG CCTAACTTGA CTCGCAGGCC ACTAAGTCCG 
AACTCGGCCT TCTCGGAGAG AACGTCGGTG TGCGGACAGG AGAAGGAGCA ACCCACTGGA 
TGCAACCAGC TCTTTGAATT CCCCGGGGGG CAGTTGCACG AGCCCTTTGA GGAAGCCGCG 
GAGTTTGCAA AAATATTACC GGACGGGCTT GACGACATCA TGAAAATGCT TGACGAGTCG 
ATAGAAAAGG AAGAGGAAGG GGTTGGGAAC GCCAGTGGGA TGGACGATAG TTCCTCCACT 
ACATCCAGCA GTCCACCAGC AGAGACCATG CCAGCATCCA GCCTAGTGAG CTCAAATCAG 
ACCACTGTTG TGAAAAGCCC ACCGCCCAAT GCCAAAACAG CGGAAGTCCG GCCAAGTGAC 
ACCTTCCCCT ATCATGGCCC TAAGAAAGCC ATGCTGGAGC AAGAAGCAAG CAAGGAGGCA 
AAGACTGCGC AGCACAGGGG AGGCAGCGTT CTGAAATCAC TCGCAAATGT TCTAGAGGGG 
CAGAAGTATT GCTACCGAGG CAGCTCTGGG GCGAAGTCCG CAGGAAACCC GGCATACTTC 
ACTGGTGCTA TGGTGAGCAA GCGTAATGTT GACCCATACG CTACGCAATC TACCTCACAC 
CGGGCCTGGG ACACCAAGCC AGGGACGCAT GTTGGGGCAG CCCCTACCTC AGAGGCTCTT 
AAGCCAGGGG TGATTGAGAA AAGGCCAGAG GTACGGACAG AGACGGACAT TCTGGAGGAC 
ATAGCGAGGG CTTGTGAGAC TTTGGTTGAG GGTGCAAAGG GGTCGGCGGC AAGCAGGACG 
GGAAACGAGA GAGCAGTTGA GAAAGAGCCC AAAAAGTCTG AAGGGATTGT GGACAGGAAG 
CCAGATTTAC AGTGGGGCAT AGAAAAACGT GACATCCATT GGGGGGCAAA TAAACTAGAA 
GGACCAGTTA AGCAGAAAGT AGATTTCAAG TGGAGAAGCG ATACCCAGAA AGAAGTGGTT 
GAGAAGAAGC CAGAGGCTCC CAGGGCTACT GGCCAACAGG ACTATGTTGA GAAGAAACAT 
GGGGCAGACA AACCCCGCGT CCCCGTTGAC AGGAAGCCTG AGACTTTGCA GAAGGCAGAT 
GTACAGGAAG GAGTTGCTGA AATAAAGCAG GAAACATCTT ATGGCACAAT CAGGCATGAA 
GTGGCAGAGA GGCTGGAGGC GGCTCTTGAG AGAGAATCCA AGAAGTCGGT GAAGGGAGCA 
GAGAAGAAAT GTCTCCCAGG TCCAGCTGAC TCCCCCTCAG GTACAGCCCG GGAAGATCCC 
TCTAAAATAC ACGGTGCTAA TGTCAAGGAG GCTGTAAAGG AGGCGACAGC TAAGCGGCTG 
CAAGGGGTAA ATGACAGAGA GTCACCAAAA AAGAACACCG ATTCTTTGGC CCAGGGAGGG 
GTCAGTAAGA ACGTGGCGGC TGAAGTGGCA AAACCGAATG CCGATAGCAA GGACACTAAG 
TCATCTGAGG AAGCACCGAT GCCAACAATG GAGGCAGGAG ACAATGGCAC AAAGGGATGG 
GAGGCTGGGA AACCCATTGA GAGGTTGACT ATATCTCTAC CTGCCCCTGC TCGGAGCAAG 
GAGGAAAGCA GGAGGAGAGA GCGGAAGCAC AGGAAGTCCA GGCGGGACTG TAGTAAGAAG 
TCAACTGACC GCAGGTCTCT GAAGGACAAG GAGAGGCAAG TCCTAGGCAA CCTAGATCTC 
CAGAGCCAGT GCCGGGACAC CTCAAATTCT ACACTTAAGC AGAGTTCAAG GAAGTCCGAG 
ACTGGCAGAA GAGGGTGCGA GGAAGCGCAT CACCACCAAC CTACCCCACC TACTCCTCCA 
ACTTCCGCAC CCCCTCCAAC GTCCCACGGC ACAGCCAACT CTGACCTTCT AAAGATGAGA 
CCCTTCACCG AAGGGCCGCC TAAAGAGCTC AAGATCCGCC TCATAAAGGT TGAAAGCGGA 
GATCGGGAGA CTTTTATAGC CTCCGAGGTG GAAGAGCGAA GGTTGCAGGC CAGTGAGCTG 
ACCATTAATA ACACTGCTGC GGAGATTGTA AGGGCCACCA GGAGTATGGT GCTAAAAGGG 
AAGTTCAAAG AGTCCTACCT GCTGCCCTCG ATGTCCGTGA AGCCGCCCTT GGGGACGGAG 
CAGAACCTTC CACGAGAGAA GCTGAATCCC CCAACTCCCA GTATATATCT GGAGAGCAAA 
CGAGATGCCT TCTCCCCTAT CCTGCTTCAG TTCTGCACCG ACCCCAAGAA TCCCATCACT 
GTTATCCGCG GTTTGGCCGG GTCCCTGCGT CTCAACCTCG GACTCTTCTC CACCAAAACG 
CTCGTTGACG CCAACGGGGA GCACATGGTG GAGGTGCGCA CTCAGGTACA ACAGCCGTCA 
GATGAGAACT GGGACCCGTC GGGCACTCGC CAGATCTGGC ACTGCGAGAG CAGCCGTTCC 
CACACCACCA TCGCCAAATA TGCACAGTAT CAGGCCTCCT CGTTCCAGGA GTCCCTGCAG 
GAGGATAAGG AAAGTGATGA CGACTCCAAT GAACCAGATA GTACCACAGA AACCCCACCC 
CCCAGTAGCA CAAACCCGGA GCACAAGACC CCACAGATTA TCAAGTTCGG CACCAATATT 
GATCTGTCTG ATCCCAAAAG GTGGAAACCT CAGTTACAGG AGCTGCAGAA GTTGCCATCT 
TTCATGCGTG TTACATCCAA TGCCAACATG CTAAGCCACG TGGGCCATAC TATACTTGGC 
ATGAACACCG TTCAGCTCTA CATGAAGGTT CCGGGCAGCA GGACCCCGGG TCACCAGGAG 
AATAACAACT TCTGCTCTGT GAATATAAAC ATCGGCCCCG GGGACTGCGA ATGGTTCGCT 
GTACATGAAA ATTACTGGGA GAAAATCAGC GCCTTCTGTG ACAAACACAA TCTGGACTAC 
CTGACCGGAT CTTGGTGGCC GGTTCTAGAA GACCTGTACC GCGCCGACAT CCCAGTTTAT 
CGGTTTGTTC AGCGCCCGGG AGACCTGGTC TGGATCAACG CCGGAACTGT GCATTGGGTG 
CAGGCTACAG GCTGGTGCAA CAACATCGCG TGGAACGTGG GGCCCCTGAC ACCCTATCAG 
TACCAACTGG CGCTGGAGCG ATTTGAGTGG AACGAAGTCA AGAACGTGAA ATCCATCGTC 
CCAATGATCC ACGTGTCCTG GAACGTGGCC CGGACCGTCA AAATCAGTGA TCCAGACCTG 
TATCGCATGA TCAAGTACTG CCTGATGCAG TCTATGAAGC GCTGCGTTTT GCAGAGGGAC 
AGCCTGATCC GCGGCGGGAA GAAAATCGCC TACCAGAGCA GAGTGAAAGA TGAACCGGCC 
TATTACTGCA ACGAGTGTGA TGTGGAGGTG TTTAACATCC TATTCGTGAC CAGCGAGAGC 
GGTGGGAAGA ACACGTACCT GGTGCACTGT GAGAGCTGCT CCCGGAGCGC CAGTAGCGCA 
AGCAGCGCCA GCAGCAACGT CGTCATCTTG GAGCAGTTTA AGACCGAGGA GCTAATGCAC 
ATATACGACA ACTTCCACCT GGCTCCACCC CCGAACATCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753881.1
CDS	542..5626
Translation	MYRSQDQFGSRFPRDSFISGVNTTWSPPHPRPWAPVSRCSSNMNQPQLPPYLPSGHVTNLGHPSKPYYPISSPPGPRIGKLERVHSCVQALLRDEQQPSLWEQLGQIYESEQEWEDAIHCYQTAARYHGGYELGARISRLQQAHMWNMHATPHHRPKSLPPLQQVWDLMQHEQKRHFGAKRCLPQLKRPVPPSDSSALQLSPHNPHGGEEQPMKRRRSRSPEQEPRPPHAPSYPPYCPPRPLPPPPPLHQQPRPPSDLSEARGQRCAEAVPPAPPPLHVPYDQRRPRATPPTTPRTDLTTSSDRYQNSAGSPSRICPPLVEQLDPPQTICQPQLPAVSRPPPPLQQGWLPPPAKEEMKKGDPLEDILYGDGPTKVKQEGFYPSRDTQREGNVYNHQPQGITTSKARSEAYQPGSLDHVLQKMDESGLIAPKKEEEIKQEVRNHPLTPELEVGGKQSSHGLSRAEGSYSGGKGSPEGADETSFCPSLAPHHKDTSGSLPVPGSVALPAKPMPNLTRRPLSPNSAFSERTSVCGQEKEQPTGCNQLFEFPGGQLHEPFEEAAEFAKILPDGLDDIMKMLDESIEKEEEGVGNASGMDDSSSTTSSSPPAETMPASSLVSSNQTTVVKSPPPNAKTAEVRPSDTFPYHGPKKAMLEQEASKEAKTAQHRGGSVLKSLANVLEGQKYCYRGSSGAKSAGNPAYFTGAMVSKRNVDPYATQSTSHRAWDTKPGTHVGAAPTSEALKPGVIEKRPEVRTETDILEDIARACETLVEGAKGSAASRTGNERAVEKEPKKSEGIVDRKPDLQWGIEKRDIHWGANKLEGPVKQKVDFKWRSDTQKEVVEKKPEAPRATGQQDYVEKKHGADKPRVPVDRKPETLQKADVQEGVAEIKQETSYGTIRHEVAERLEAALERESKKSVKGAEKKCLPGPADSPSGTAREDPSKIHGANVKEAVKEATAKRLQGVNDRESPKKNTDSLAQGGVSKNVAAEVAKPNADSKDTKSSEEAPMPTMEAGDNGTKGWEAGKPIERLTISLPAPARSKEESRRRERKHRKSRRDCSKKSTDRRSLKDKERQVLGNLDLQSQCRDTSNSTLKQSSRKSETGRRGCEEAHHHQPTPPTPPTSAPPPTSHGTANSDLLKMRPFTEGPPKELKIRLIKVESGDRETFIASEVEERRLQASELTINNTAAEIVRATRSMVLKGKFKESYLLPSMSVKPPLGTEQNLPREKLNPPTPSIYLESKRDAFSPILLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKTLVDANGEHMVEVRTQVQQPSDENWDPSGTRQIWHCESSRSHTTIAKYAQYQASSFQESLQEDKESDDDSNEPDSTTETPPPSSTNPEHKTPQIIKFGTNIDLSDPKRWKPQLQELQKLPSFMRVTSNANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHENYWEKISAFCDKHNLDYLTGSWWPVLEDLYRADIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTPYQYQLALERFEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLYRMIKYCLMQSMKRCVLQRDSLIRGGKKIAYQSRVKDEPAYYCNECDVEVFNILFVTSESGGKNTYLVHCESCSRSASSASSASSNVVILEQFKTEELMHIYDNFHLAPPPNIR

Target ORF information:

RefSeq Version	XM_031898021.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031898021.1

ORF Insert Sequence:

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATC AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ATGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA GCGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGG AACCCAGACC CCCCCACGCG CCGTCTTACC CACCATATTG CCCTCCACGC 
CCTCTACCGC CTCCACCACC ACTTCACCAG CAGCCCCGCC CTCCAAGTGA CCTTAGTGAG 
GCTCGGGGGC AGAGGTGTGC AGAGGCCGTC CCCCCAGCAC CGCCCCCTCT CCACGTGCCT 
TACGATCAGA GAAGACCCCG CGCCACCCCG CCGACTACCC CCAGGACCGA TCTCACCACT 
TCCTCTGATC GCTACCAGAA TTCCGCAGGC AGCCCGTCTA GAATCTGCCC TCCTCTTGTA 
GAGCAGCTGG ACCCCCCACA AACTATATGT CAACCCCAGC TACCAGCTGT CTCTCGACCT 
CCTCCTCCTC TGCAACAGGG ATGGCTGCCC CCCCCAGCCA AAGAGGAGAT GAAGAAGGGA 
GACCCACTAG AGGACATTCT GTATGGAGAT GGACCTACAA AGGTTAAACA GGAAGGGTTT 
TACCCATCCC GTGACACCCA AAGAGAGGGC AATGTCTACA ACCACCAACC TCAGGGCATT 
ACCACCAGCA AAGCCAGGTC TGAGGCCTAC CAGCCTGGAA GCCTGGATCA CGTACTGCAG 
AAGATGGATG AATCTGGGTT AATTGCCCCA AAGAAGGAGG AAGAGATTAA ACAGGAAGTT 
AGAAATCATC CATTGACCCC AGAACTGGAG GTTGGAGGCA AACAATCAAG CCATGGACTC 
TCTAGAGCTG AGGGCAGCTA TTCTGGTGGG AAGGGGTCAC CAGAAGGCGC GGATGAAACG 
TCTTTCTGCC CCTCTTTGGC CCCCCACCAC AAAGATACCT CAGGGAGCCT CCCGGTTCCA 
GGCTCAGTTG CATTACCAGC CAAGCCAATG CCTAACTTGA CTCGCAGGCC ACTAAGTCCG 
AACTCGGCCT TCTCGGAGAG AACGTCGGTG TGCGGACAGG AGAAGGAGCA ACCCACTGGA 
TGCAACCAGC TCTTTGAATT CCCCGGGGGG CAGTTGCACG AGCCCTTTGA GGAAGCCGCG 
GAGTTTGCAA AAATATTACC GGACGGGCTT GACGACATCA TGAAAATGCT TGACGAGTCG 
ATAGAAAAGG AAGAGGAAGG GGTTGGGAAC GCCAGTGGGA TGGACGATAG TTCCTCCACT 
ACATCCAGCA GTCCACCAGC AGAGACCATG CCAGCATCCA GCCTAGTGAG CTCAAATCAG 
ACCACTGTTG TGAAAAGCCC ACCGCCCAAT GCCAAAACAG CGGAAGTCCG GCCAAGTGAC 
ACCTTCCCCT ATCATGGCCC TAAGAAAGCC ATGCTGGAGC AAGAAGCAAG CAAGGAGGCA 
AAGACTGCGC AGCACAGGGG AGGCAGCGTT CTGAAATCAC TCGCAAATGT TCTAGAGGGG 
CAGAAGTATT GCTACCGAGG CAGCTCTGGG GCGAAGTCCG CAGGAAACCC GGCATACTTC 
ACTGGTGCTA TGGTGAGCAA GCGTAATGTT GACCCATACG CTACGCAATC TACCTCACAC 
CGGGCCTGGG ACACCAAGCC AGGGACGCAT GTTGGGGCAG CCCCTACCTC AGAGGCTCTT 
AAGCCAGGGG TGATTGAGAA AAGGCCAGAG GTACGGACAG AGACGGACAT TCTGGAGGAC 
ATAGCGAGGG CTTGTGAGAC TTTGGTTGAG GGTGCAAAGG GGTCGGCGGC AAGCAGGACG 
GGAAACGAGA GAGCAGTTGA GAAAGAGCCC AAAAAGTCTG AAGGGATTGT GGACAGGAAG 
CCAGATTTAC AGTGGGGCAT AGAAAAACGT GACATCCATT GGGGGGCAAA TAAACTAGAA 
GGACCAGTTA AGCAGAAAGT AGATTTCAAG TGGAGAAGCG ATACCCAGAA AGAAGTGGTT 
GAGAAGAAGC CAGAGGCTCC CAGGGCTACT GGCCAACAGG ACTATGTTGA GAAGAAACAT 
GGGGCAGACA AACCCCGCGT CCCCGTTGAC AGGAAGCCTG AGACTTTGCA GAAGGCAGAT 
GTACAGGAAG GAGTTGCTGA AATAAAGCAG GAAACATCTT ATGGCACAAT CAGGCATGAA 
GTGGCAGAGA GGCTGGAGGC GGCTCTTGAG AGAGAATCCA AGAAGTCGGT GAAGGGAGCA 
GAGAAGAAAT GTCTCCCAGG TCCAGCTGAC TCCCCCTCAG GTACAGCCCG GGAAGATCCC 
TCTAAAATAC ACGGTGCTAA TGTCAAGGAG GCTGTAAAGG AGGCGACAGC TAAGCGGCTG 
CAAGGGGTAA ATGACAGAGA GTCACCAAAA AAGAACACCG ATTCTTTGGC CCAGGGAGGG 
GTCAGTAAGA ACGTGGCGGC TGAAGTGGCA AAACCGAATG CCGATAGCAA GGACACTAAG 
TCATCTGAGG AAGCACCGAT GCCAACAATG GAGGCAGGAG ACAATGGCAC AAAGGGATGG 
GAGGCTGGGA AACCCATTGA GAGGTTGACT ATATCTCTAC CTGCCCCTGC TCGGAGCAAG 
GAGGAAAGCA GGAGGAGAGA GCGGAAGCAC AGGAAGTCCA GGCGGGACTG TAGTAAGAAG 
TCAACTGACC GCAGGTCTCT GAAGGACAAG GAGAGGCAAG TCCTAGGCAA CCTAGATCTC 
CAGAGCCAGT GCCGGGACAC CTCAAATTCT ACACTTAAGC AGAGTTCAAG GAAGTCCGAG 
ACTGGCAGAA GAGGGTGCGA GGAAGCGCAT CACCACCAAC CTACCCCACC TACTCCTCCA 
ACTTCCGCAC CCCCTCCAAC GTCCCACGGC ACAGCCAACT CTGACCTTCT AAAGATGAGA 
CCCTTCACCG AAGGGCCGCC TAAAGAGCTC AAGATCCGCC TCATAAAGGT TGAAAGCGGA 
GATCGGGAGA CTTTTATAGC CTCCGAGGTG GAAGAGCGAA GGTTGCAGGC CAGTGAGCTG 
ACCATTAATA ACACTGCTGC GGAGATTGTA AGGGCCACCA GGAGTATGGT GCTAAAAGGG 
AAGTTCAAAG AGTCCTACCT GCTGCCCTCG ATGTCCGTGA AGCCGCCCTT GGGGACGGAG 
CAGAACCTTC CACGAGAGAA GCTGAATCCC CCAACTCCCA GTATATATCT GGAGAGCAAA 
CGAGATGCCT TCTCCCCTAT CCTGCTTCAG TTCTGCACCG ACCCCAAGAA TCCCATCACT 
GTTATCCGCG GTTTGGCCGG GTCCCTGCGT CTCAACCTCG GACTCTTCTC CACCAAAACG 
CTCGTTGACG CCAACGGGGA GCACATGGTG GAGGTGCGCA CTCAGGTACA ACAGCCGTCA 
GATGAGAACT GGGACCCGTC GGGCACTCGC CAGATCTGGC ACTGCGAGAG CAGCCGTTCC 
CACACCACCA TCGCCAAATA TGCACAGTAT CAGGCCTCCT CGTTCCAGGA GTCCCTGCAG 
GAGGATAAGG AAAGTGATGA CGACTCCAAT GAACCAGATA GTACCACAGA AACCCCACCC 
CCCAGTAGCA CAAACCCGGA GCACAAGACC CCACAGATTA TCAAGTTCGG CACCAATATT 
GATCTGTCTG ATCCCAAAAG GTGGAAACCT CAGTTACAGG AGCTGCAGAA GTTGCCATCT 
TTCATGCGTG TTACATCCAA TGCCAACATG CTAAGCCACG TGGGCCATAC TATACTTGGC 
ATGAACACCG TTCAGCTCTA CATGAAGGTT CCGGGCAGCA GGACCCCGGG TCACCAGGAG 
AATAACAACT TCTGCTCTGT GAATATAAAC ATCGGCCCCG GGGACTGCGA ATGGTTCGCT 
GTACATGAAA ATTACTGGGA GAAAATCAGC GCCTTCTGTG ACAAACACAA TCTGGACTAC 
CTGACCGGAT CTTGGTGGCC GGTTCTAGAA GACCTGTACC GCGCCGACAT CCCAGTTTAT 
CGGTTTGTTC AGCGCCCGGG AGACCTGGTC TGGATCAACG CCGGAACTGT GCATTGGGTG 
CAGGCTACAG GCTGGTGCAA CAACATCGCG TGGAACGTGG GGCCCCTGAC ACCCTATCAG 
TACCAACTGG CGCTGGAGCG ATTTGAGTGG AACGAAGTCA AGAACGTGAA ATCCATCGTC 
CCAATGATCC ACGTGTCCTG GAACGTGGCC CGGACCGTCA AAATCAGTGA TCCAGACCTG 
TATCGCATGA TCAAGTACTG CCTGATGCAG TCTATGAAGC GCTGCGTTTT GCAGAGGGAC 
AGCCTGATCC GCGGCGGGAA GAAAATCGCC TACCAGAGCA GAGTGAAAGA TGAACCGGCC 
TATTACTGCA ACGAGTGTGA TGTGGAGGTG TTTAACATCC TATTCGTGAC CAGCGAGAGC 
GGTGGGAAGA ACACGTACCT GGTGCACTGT GAGAGCTGCT CCCGGAGCGC CAGTAGCGCA 
AGCAGCGCCA GCAGCAACGT CGTCATCTTG GAGCAGTTTA AGACCGAGGA GCTAATGCAC 
ATATACGACA ACTTCCACCT GGCTCCACCC CCGAACATCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6b ( NM_001278756.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001265685.1 Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), mRNA.

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CloneID	OXa20376
Clone ID Related Accession (Same CDS sequence)	NM_001278756.1
Accession Version	NM_001278756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	lysine-specific demethylase 6B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JN107757.1. On or before Jul 15, 2013 this sequence version replaced XM_002943186.2, XM_004913200.1, XM_004913201.1, XM_004913202.1, XM_004913203.1, XM_004913204.1. ##Evidence-Data-START## Transcript exon combination :: JN107757.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00861997 [ECO:0000348] ##Evidence-Data-END##

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATA AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ACGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA ACGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT 
GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG 
CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA 
CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT 
CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG 
CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC 
AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA 
ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA 
CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA 
GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG 
GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC 
CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC 
CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA 
GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA 
CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA 
GAAGGCGCGG ATGAAACGTC TTTTTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA 
GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT 
CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG 
AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG 
CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG 
AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGACG 
GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC 
CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCCC CGCCCAATGC CAAAACAGCG 
GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA 
GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC 
GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA 
GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT 
ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC 
CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG 
ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG 
TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA 
GGGATTGTGG ACCGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG 
GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT 
ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC 
TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGTGTCC CCGTTGACAG GAAGCCTGAG 
ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT 
GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG 
AAGTCAGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT 
ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG 
GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT 
TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC 
GATAGCAAGG ACACTAAGTC ATCTGAGGAT GCACCGATGC CAACAATGGA GGCAGGAGAC 
AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT 
GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG 
CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC 
CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG 
AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT 
ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT 
GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC 
ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG 
TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG 
AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG 
CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT 
ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC 
CCCAAGAATC CCATCACTGT TATCCGTGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA 
CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT 
CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC 
TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG 
TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT 
ACCACAGAAA CCCCACCCCC CAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG 
TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG 
CAGAAGTTGC CGTCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC 
CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC 
CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC 
TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA 
CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC 
GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA 
ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC 
CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC 
GTGAAATCCA TTGTCCCGAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC 
AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC 
GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG 
AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC 
GTGACCAGCG AGAGCGGCGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG 
AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTCAAGACC 
GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265685.1
CDS	234..5453
Translation	MYRSQDQFGSRFPRDSFISGVNTTWSPPHPRPWAPVSRCSSNMNQPQLPPYLPSGHVTNLGHPSKPYYPISSPPGPRIGKLERVHSCVQALLRDEQQPSLWEQLGQIYESEQEWEDAIHCYQTAARYHGGYELGARISRLQQAHMWNMHATPHHRPKSLPPLQQVWDLMQHEQKRHFGAKRCLPQLKRPVPPSDSSALQLSPHNPHGGEEQPMKRRRSRSPEQQRRGNPGLHSPPASGGSGSLYHQAPVKPSVWNPLTHVPWAERRERQEPRPPHAPSYPPYCPPRPLPPPPPLHQQPRPPSDLSEARGQRCAEAVPPAPPPLHVPYDQRRPRATPPTTPRTDLTTSSDRYQNSAGSPSRICPPLVEQLDPPQTICQPQLPAVSRPPPPLQQGWLPPPAKEEMKKGDPLEDILYGDGPTKVKQEGFYPSRDTQREGNVYNHQPQGITTSKARSEAYQPGSLDHVLQKMDESGLIAPKKEEEIKQEVRNHPLTPELEVGGKQSSHGLSRAEGSYSGGKGSPEGADETSFCPSLAPHHKDTSGSLPVPGSVALPAKPMPNLTRRPLSPNSAFSERTSVCGQEKEQPTGCNQLFEFPGGQLHEPFEEAAEFAKILPDGLDDIMKMLDESIEKEEEGVGNASGTDDSSSTTSSSPPAETMPASSLVSSNQTTVVKSPPPNAKTAEVRPSDTFPYHGPKKAMLEQEASKEAKTAQHRGGSVLKSLANVLEGQKYCYRGSSGAKSAGNPAYFTGAMVSKRNVDPYATQSTSHRAWDTKPGTHVGAAPTSEALKPGVIEKRPEVRTETDILEDIARACETLVEGAKGSAASRTGNERAVEKEPKKSEGIVDRKPDLQWGIEKRDIHWGANKLEGPVKQKVDFKWRSDTQKEVVEKKPEAPRATGQQDYVEKKHGADKPRVPVDRKPETLQKADVQEGVAEIKQETSYGTIRHEVAERLEAALERESKKSVKGAEKKCLPGPADSPSGTAREDPSKIHGANVKEAVKEATAKRLQGVNDRESPKKNTDSLAQGGVSKNVAAEVAKPNADSKDTKSSEDAPMPTMEAGDNGTKGWEAGKPIERLTISLPAPARSKEESRRRERKHRKSRRDCSKKSTDRRSLKDKERQVLGNLDLQSQCRDTSNSTLKQSSRKSETGRRGCEEAHHHQPTPPTPPTSAPPPTSHGTANSDLLKMRPFTEGPPKELKIRLIKVESGDRETFIASEVEERRLQASELTINNTAAEIVRATRSMVLKGKFKESYLLPSMSVKPPLGTEQNLPREKLNPPTPSIYLESKRDAFSPILLQFCTDPKNPITVIRGLAGSLRLNLGLFSTKTLVDANGEHMVEVRTQVQQPSDENWDPSGTRQIWHCESSRSHTTIAKYAQYQASSFQESLQEDKESDDDSNEPDSTTETPPPSTNPEHKTPQIIKFGTNIDLSDPKRWKPQLQELQKLPSFMRVTSNANMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVHENYWEKISAFCDKHNLDYLTGSWWPVLEDLYRADIPVYRFVQRPGDLVWINAGTVHWVQATGWCNNIAWNVGPLTPYQYQLALERFEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLYRMIKYCLMQSMKRCVLQRDSLIRGGKKIAYQSRVKDEPAYYCNECDVEVFNILFVTSESGGKNTYLVHCESCSRSASSASSASSNVVILEQFKTEELMHIYDNFHLAPPPNIR

Target ORF information:

RefSeq Version	NM_001278756.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis lysine (K)-specific demethylase 6B (kdm6b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278756.1

ORF Insert Sequence:

ATGTATCGCT CACAGGATCA GTTTGGCTCT CGCTTTCCCC GAGATTCCTT TATCAGTGGA 
GTCAACACAA CATGGAGCCC GCCCCACCCC AGGCCATGGG CCCCCGTGAG CAGGTGTTCT 
TCAAACATGA ATCAGCCCCA GCTGCCCCCT TACCTGCCCT CTGGCCATGT GACTAACCTT 
GGGCATCCCA GCAAACCTTA TTACCCAATA AGCTCACCCC CCGGCCCCCG CATTGGGAAG 
TTGGAGCGGG TACACTCCTG CGTGCAAGCC CTGCTGCGGG ACGAGCAGCA GCCCTCCCTC 
TGGGAGCAGC TGGGTCAGAT CTACGAGTCG GAACAGGAAT GGGAGGACGC CATTCACTGC 
TATCAGACGG CTGCCCGCTA TCATGGTGGC TACGAGCTTG GCGCGCGCAT CTCCAGGCTG 
CAGCAGGCTC ACATGTGGAA CATGCACGCA ACCCCCCATC ATCGGCCCAA ATCTCTGCCC 
CCCCTGCAAC AAGTCTGGGA CCTTATGCAA CACGAGCAGA AGAGACATTT CGGAGCTAAA 
CGGTGTCTCC CGCAGCTGAA ACGCCCGGTG CCCCCCTCAG ACTCTTCCGC ACTCCAGCTG 
AGCCCACACA ATCCCCATGG AGGGGAGGAG CAACCGATGA AACGGAGGAG GAGCCGGAGC 
CCCGAACAGC AAAGGAGGGG GAACCCTGGC CTGCACTCTC CTCCAGCCTC AGGGGGTTCT 
GGGAGTTTGT ACCATCAGGC ACCCGTCAAG CCCAGTGTTT GGAATCCACT CACCCATGTG 
CCCTGGGCTG AGCGCAGGGA GCGGCAGGAA CCCAGACCCC CCCACGCGCC GTCTTACCCA 
CCATATTGCC CTCCACGCCC TCTACCGCCT CCACCACCAC TTCACCAGCA GCCCCGCCCT 
CCAAGTGACC TTAGTGAGGC TCGGGGGCAG AGGTGTGCAG AGGCCGTCCC CCCAGCACCG 
CCCCCTCTCC ACGTGCCTTA CGATCAGAGA AGACCCCGCG CCACCCCGCC GACTACCCCC 
AGGACCGATC TCACCACTTC CTCTGATCGC TACCAGAATT CCGCAGGCAG CCCGTCTAGA 
ATCTGCCCTC CTCTTGTAGA GCAGCTGGAC CCCCCACAAA CTATATGTCA ACCCCAGCTA 
CCAGCTGTCT CTCGACCTCC TCCTCCTCTG CAACAGGGAT GGCTGCCCCC CCCAGCCAAA 
GAGGAGATGA AGAAGGGAGA CCCACTAGAG GACATTCTGT ATGGAGATGG ACCTACAAAG 
GTTAAACAGG AAGGGTTTTA CCCATCCCGT GACACCCAAA GAGAGGGCAA TGTCTACAAC 
CACCAACCTC AGGGCATTAC CACCAGCAAA GCCAGGTCTG AGGCCTACCA GCCTGGAAGC 
CTGGATCACG TACTGCAGAA GATGGATGAA TCTGGGTTAA TTGCCCCAAA GAAGGAGGAA 
GAGATTAAAC AGGAAGTTAG AAATCATCCA TTGACCCCAG AACTGGAGGT TGGAGGCAAA 
CAATCAAGCC ATGGACTCTC TAGAGCTGAG GGCAGCTATT CTGGTGGGAA GGGGTCACCA 
GAAGGCGCGG ATGAAACGTC TTTTTGCCCC TCTTTGGCCC CCCACCACAA AGATACCTCA 
GGGAGCCTCC CGGTTCCAGG CTCAGTTGCA TTACCAGCCA AGCCAATGCC TAACTTGACT 
CGCAGGCCAC TAAGTCCGAA CTCGGCCTTC TCGGAGAGAA CGTCGGTGTG CGGACAGGAG 
AAGGAGCAAC CCACTGGATG CAACCAGCTC TTTGAATTCC CCGGGGGGCA GTTGCACGAG 
CCCTTTGAGG AAGCCGCGGA GTTTGCAAAA ATATTACCGG ACGGGCTTGA CGACATCATG 
AAAATGCTTG ACGAGTCGAT AGAAAAGGAA GAGGAAGGGG TTGGGAACGC CAGTGGGACG 
GACGATAGTT CCTCCACTAC ATCCAGCAGT CCACCAGCAG AGACCATGCC AGCATCCAGC 
CTAGTGAGCT CAAATCAGAC CACTGTTGTG AAAAGCCCCC CGCCCAATGC CAAAACAGCG 
GAAGTCCGGC CAAGTGACAC CTTCCCCTAT CATGGCCCTA AGAAAGCCAT GCTGGAGCAA 
GAAGCAAGCA AGGAGGCAAA GACTGCGCAG CACAGGGGAG GCAGCGTTCT GAAATCACTC 
GCAAATGTTC TAGAGGGGCA GAAGTATTGC TACCGAGGCA GCTCTGGGGC GAAGTCCGCA 
GGAAACCCGG CATACTTCAC TGGTGCTATG GTGAGCAAGC GTAATGTTGA CCCATACGCT 
ACGCAATCTA CCTCACACCG GGCCTGGGAC ACCAAGCCAG GGACGCATGT TGGGGCAGCC 
CCTACCTCAG AGGCTCTTAA GCCAGGGGTG ATTGAGAAAA GGCCAGAGGT ACGGACAGAG 
ACGGACATTC TGGAGGACAT AGCGAGGGCT TGTGAGACTT TGGTTGAGGG TGCAAAGGGG 
TCGGCGGCAA GCAGGACGGG AAACGAGAGA GCAGTTGAGA AAGAGCCCAA AAAGTCTGAA 
GGGATTGTGG ACCGGAAGCC AGATTTACAG TGGGGCATAG AAAAACGTGA CATCCATTGG 
GGGGCAAATA AACTAGAAGG ACCAGTTAAG CAGAAAGTAG ATTTCAAGTG GAGAAGCGAT 
ACCCAGAAAG AAGTGGTTGA GAAGAAGCCA GAGGCTCCCA GGGCTACTGG CCAACAGGAC 
TATGTTGAGA AGAAACATGG GGCAGACAAA CCCCGTGTCC CCGTTGACAG GAAGCCTGAG 
ACTTTGCAGA AGGCAGATGT ACAGGAAGGA GTTGCTGAAA TAAAGCAGGA AACATCTTAT 
GGCACAATCA GGCATGAAGT GGCAGAGAGG CTGGAGGCGG CTCTTGAGAG AGAATCCAAG 
AAGTCAGTGA AGGGAGCAGA GAAGAAATGT CTCCCAGGTC CAGCTGACTC CCCCTCAGGT 
ACAGCCCGGG AAGATCCCTC TAAAATACAC GGTGCTAATG TCAAGGAGGC TGTAAAGGAG 
GCGACAGCTA AGCGGCTGCA AGGGGTAAAT GACAGAGAGT CACCAAAAAA GAACACCGAT 
TCTTTGGCCC AGGGAGGGGT CAGTAAGAAC GTGGCGGCTG AAGTGGCAAA ACCGAATGCC 
GATAGCAAGG ACACTAAGTC ATCTGAGGAT GCACCGATGC CAACAATGGA GGCAGGAGAC 
AATGGCACAA AGGGATGGGA GGCTGGGAAA CCCATTGAGA GGTTGACTAT ATCTCTACCT 
GCCCCTGCTC GGAGCAAGGA GGAAAGCAGG AGGAGAGAGC GGAAGCACAG GAAGTCCAGG 
CGGGACTGTA GTAAGAAGTC AACTGACCGC AGGTCTCTGA AGGACAAGGA GAGGCAAGTC 
CTAGGCAACC TAGATCTCCA GAGCCAGTGC CGGGACACCT CAAATTCTAC ACTTAAGCAG 
AGTTCAAGGA AGTCCGAGAC TGGCAGAAGA GGGTGCGAGG AAGCGCATCA CCACCAACCT 
ACCCCACCTA CTCCTCCAAC TTCCGCACCC CCTCCAACGT CCCACGGCAC AGCCAACTCT 
GACCTTCTAA AGATGAGACC CTTCACCGAA GGGCCGCCTA AAGAGCTCAA GATCCGCCTC 
ATAAAGGTTG AAAGCGGAGA TCGGGAGACT TTTATAGCCT CCGAGGTGGA AGAGCGAAGG 
TTGCAGGCCA GTGAGCTGAC CATTAATAAC ACTGCTGCGG AGATTGTAAG GGCCACCAGG 
AGTATGGTGC TAAAAGGGAA GTTCAAAGAG TCCTACCTGC TGCCCTCGAT GTCCGTGAAG 
CCGCCCTTGG GGACGGAGCA GAACCTTCCA CGAGAGAAGC TGAATCCCCC AACTCCCAGT 
ATATATCTGG AGAGCAAACG AGATGCCTTC TCCCCTATCC TGCTTCAGTT CTGCACCGAC 
CCCAAGAATC CCATCACTGT TATCCGTGGT TTGGCCGGGT CCCTGCGTCT CAACCTCGGA 
CTCTTCTCCA CCAAAACGCT CGTTGACGCC AACGGGGAGC ACATGGTGGA GGTGCGCACT 
CAGGTACAAC AGCCGTCAGA TGAGAACTGG GACCCGTCGG GCACTCGCCA GATCTGGCAC 
TGCGAGAGCA GCCGTTCCCA CACCACCATC GCCAAATATG CACAGTATCA GGCCTCCTCG 
TTCCAGGAGT CCCTGCAGGA GGATAAGGAA AGTGATGACG ACTCCAATGA ACCAGATAGT 
ACCACAGAAA CCCCACCCCC CAGCACAAAC CCGGAGCACA AGACCCCACA GATTATCAAG 
TTCGGCACCA ATATTGATCT GTCTGATCCC AAAAGGTGGA AACCTCAGTT ACAGGAGCTG 
CAGAAGTTGC CGTCTTTCAT GCGTGTTACA TCCAATGCCA ACATGCTAAG CCACGTGGGC 
CATACTATAC TTGGCATGAA CACCGTTCAG CTCTACATGA AGGTTCCGGG CAGCAGGACC 
CCGGGTCACC AGGAGAATAA CAACTTCTGC TCTGTGAATA TAAACATCGG CCCCGGGGAC 
TGCGAATGGT TCGCTGTACA TGAAAATTAC TGGGAGAAAA TCAGCGCCTT CTGTGACAAA 
CACAATCTGG ACTACCTGAC CGGATCTTGG TGGCCGGTTC TAGAAGACCT GTACCGCGCC 
GACATCCCAG TTTATCGGTT TGTTCAGCGC CCGGGAGACC TGGTCTGGAT CAACGCCGGA 
ACTGTGCATT GGGTGCAGGC TACAGGCTGG TGCAACAACA TCGCGTGGAA CGTGGGGCCC 
CTGACACCCT ATCAGTACCA ACTGGCGCTG GAGCGATTTG AGTGGAACGA AGTCAAGAAC 
GTGAAATCCA TTGTCCCGAT GATCCACGTG TCCTGGAACG TGGCCCGGAC CGTCAAAATC 
AGTGATCCAG ACCTGTATCG CATGATCAAG TACTGCCTGA TGCAGTCTAT GAAGCGCTGC 
GTTTTGCAGA GGGACAGCCT GATCCGCGGC GGGAAGAAAA TCGCCTACCA GAGCAGAGTG 
AAAGATGAAC CGGCCTATTA CTGCAACGAG TGTGATGTGG AGGTGTTTAA CATCCTATTC 
GTGACCAGCG AGAGCGGCGG GAAGAACACG TACCTGGTGC ACTGTGAGAG CTGCTCCCGG 
AGCGCCAGTA GCGCAAGCAG CGCCAGCAGC AACGTCGTCA TCTTGGAGCA GTTCAAGACC 
GAGGAGCTAA TGCACATATA CGACAACTTC CACCTGGCTC CACCCCCGAA CATCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative expression analysis of the H3K27 demethylases, JMJD3 and UTX, with the H3K27 methylase, EZH2, in Xenopus.
The International journal of developmental biology56(4)295-300(2012)
Kawaguchi A,Ochi H,Sudou N,Ogino H

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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kdm6b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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