cul1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cul1
Entrez Gene ID	734121
Full Name	cullin 1
Synonyms	cullin-1
General protein information	Preferred Names cullin 1 Names cullin-1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012964856.2	XP_012820310.1	cullin-1 isoform X1
XM_012964857.2	XP_012820311.1	cullin-1 isoform X1
XM_012964854.3	XP_012820308.1	cullin-1 isoform X1
XM_012964856.3	XP_012820310.1	cullin-1 isoform X1
NM_001045790.1	NP_001039255.1	cullin-1
XM_012964854.2	XP_012820308.1	cullin-1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cul1	NP_036172.1
Danio rerio (zebrafish)	cul1b	NP_998660.1
Neurospora crassa	NCU05204	XP_962203.2
Xenopus tropicalis (tropical clawed frog)	cul1	NP_001039255.1
	CUL1	XP_002803558.1
Canis lupus familiaris (dog)	CUL1	XP_848402.1
Bos taurus (cattle)	CUL1	NP_001180162.1
Schizosaccharomyces pombe (fission yeast)	cul1	NP_594259.1
Magnaporthe oryzae (rice blast fungus)	MGG_07145	XP_003715342.1
Rattus norvegicus (Norway rat)	Cul1	NP_001102097.1
Gallus gallus (chicken)	CUL1	XP_418878.3
Drosophila melanogaster (fruit fly)	lin19	NP_724622.1
Pan troglodytes (chimpanzee)	CUL1	NP_001233533.1
Caenorhabditis elegans (roundworm)	cul-1	NP_499309.1
Homo sapiens (human)	CUL1	NP_003583.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008007	XP_317457.4

Gene Ontology
Bibliography

Related articles in PubMed

A dominant-negative form of the E3 ubiquitin ligase Cullin-1 disrupts the correct allocation of cell fate in the neural crest lineage.
Voigt J, Papalopulu N
Development (Cambridge, England)133(3)559-68(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cul1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cul1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa25199	NM_001045790.1 Latest version!	Xenopus tropicalis cullin 1 (cul1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa28859	XM_012964854.2 Latest version!	Xenopus tropicalis cullin 1 (cul1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa28859	XM_012964857.2 Latest version!	Xenopus tropicalis cullin 1 (cul1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa28859	XM_012964856.2 Latest version!	Xenopus tropicalis cullin 1 (cul1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa25199	XM_012964856.3 Latest version!	Xenopus tropicalis cullin 1 (cul1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa25199	XM_012964854.3 Latest version!	Xenopus tropicalis cullin 1 (cul1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cul1 ( NM_001045790.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001039255.1 Xenopus tropicalis cullin 1 (cul1), mRNA.

Price & Availability↑

CloneID	OXa25199
Clone ID Related Accession (Same CDS sequence)	XM_012964854.3 , XM_012964856.3 , NM_001045790.1
Accession Version	NM_001045790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cullin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ466084.1. ##Evidence-Data-START## Transcript exon combination :: DQ466084.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGTCTTCAA ACAGAAGTCA GAATCCACAT GGACTGAAAC AGATTGGCTT GGACCAAATA 
TGGGATGACC TGCGGGCCGG GATCCAACAA GTTTATACAA GACAGAGCAT GGCAAAGTCC 
AGATATATGG AACTCTATAC ACATGTTTAT AACTACTGCA CCAGTGTACA CCAATCTAAC 
CAAGCACGGG GGGCTGGAGT GCCGCCATCA AAATCTAAGA AAGGACAGAC TCCTGGAGGT 
GCCCAGTTTG TTGGTTTGGA GCTCTACAAA AGACTAAAGG AGTTCTTAAA GAATTACTTG 
ACAAATCTCC TAAAGGATGG AGAAGATTTG ATGGATGAGA GCGTACTGAA GTTTTACACA 
CAGCAATGGG AAGATTACAG GTTTTCAAGT AAAGTGCTGA ATGGAATCTG CGCATACCTC 
AACCGGCACT GGGTTCGCCG TGAGTGTGAT GAAGGCCGCA AAGGAATATA TGAAATATAT 
TCTCTGGCCT TAGTGACATG GAGGGATTGT CTGTTTAAGC CTTTGAATAA GCAGGTAACA 
AATGCTGTGC TGAAACTGAT TGAAAAGGAA CGAAATGGTG AAACAATAAA CACCAGGCTT 
ATAAGTGGAG TGGTGCAATC TTATGTTGAG CTTGGCTTGA ATGAAGATGA TGCATTTGCC 
AAAGGCCCAA CACTGACTGT TTATAAAGAG TCCTTTGAGT CTCAGTTTTT GGCAGACACA 
GAACGGTTTT ATACAAGAGA GAGCACAGAG TTTTTACAGC AGAACCCTGT CACAGAATAT 
ATGAAAAAGG CAGAAGCTCG GCTTTTGGAG GAGCAGCGCA GAGTACAAGT ATACTTACAT 
GAGAGCACAC AGGATGAACT AGCACGGAAA TGTGAACAAG TCCTAATTGA AAAACATCTA 
GAGATTTTCC ACACTGAATT TCAGAATTTA CTAGATGCTG ATAAAAATGA AGACCTGGGC 
CGTATGTACA ATCTTGTTTC CCGAATCCAG GATGGTCTGG GAGAGCTGAA AAAGCTTTTA 
GAAACTCATA TTCACAATCA GGGACTTGCT GCCATAGAAA AATGTGGAGA AGCTGCACAA 
AATGATCCTA AAATGTATGT ACAGACTGTT TTGGATGTTC ACAAGAAATA CAATGCCTTA 
GTCATGTCAG CATTCAACAA TGATGCTGGA TTTGTGGCTG CACTAGATAA GGCTTGTGGT 
CGGTTTATAA ATAACAATGC TGTGACAAAA ATGGCTCAGT CTTCTAGCAA GTCACCAGAG 
TTGCTTGCCC GGTATTGTGA CTCTCTCTTG AAGAAGAGTT CCAAGAATCC AGAAGAAGCA 
GAGTTGGAGG ATACCCTTAA CCAAGTGATG GTGGTCTTTA AATACATAGA AGACAAGGAT 
GTGTTTCAGA AGTTCTATGC AAAAATGCTT GCAAAGCGAT TGGTTCATCA AAACAGTGCC 
AGTGATGATG CTGAAGCAAG TATGATATCA AAACTTAAGC AAGCCTGTGG GTTTGAGTAT 
ACATCAAAGC TTCAGAGAAT GTTTCAAGAT ATAGGTGTGA GCAAAGACCT TAATGAGCAG 
TTCAAAAAGC ACTTGACTAA TTCTGAGCCT TTAGACTTGG ACTTCAGCAT ACAGGTTCTC 
AGCTCTGGGT CATGGCCCTT CCAACAGTTC TGTACATTTG CTTTACCTTC AGAGTTGGAA 
CGCAGCTATC AAAGGTTTAC AGCTTTTTAT GCCAGCCGCC ACAGTGGAAG GAAGTTGACT 
TGGTTATACC AGTTGTCTAA AGGAGAACTG GTCACAAACT GCTTTAAAAA TAGATACACG 
TTGCAGGCAT CCACATTCCA GATGGCAATC CTGCTGCAGT ATAACACAGA AGATGCCTAC 
ACAGTGCAGC AACTGACAGA TAGCACACAG ATAAAAATGG ATATACTTGT GCAAGTTCTT 
CAAATTTTGC TGAAGTCTAA GTTATTGGTT TTAGAAGATG AAAATGCAAA TGTGGATGAT 
GTAGACTTGA AGCCAGATAT TTTAATAAAG CTCTACCTTG GTTATAAGAA TAAGAAGTTA 
AGAGTGAACA TAAATGTGCC CATGAAAACA GAACAGAAGC AGGAACAAGA AACAACACAC 
AAAAATATAG AGGAAGACCG AAAACTACTC ATTCAGGCAG CCATTGTGAG AATAATGAAG 
ATGAGGAAGG TTCTGAAACA CCAGCAGCTT CTTGGAGAAG TGTTGACTCA GCTGTCCTCC 
AGATTCAAAC CACGGGTCCC TGTTATCAAG AAATGCATTG ATATCCTAAT AGAAAAAGAG 
TATTTGGAGC GCGTGGATGG TGAAAAGGAC ACATACAGTT ACCTGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039255.1
CDS	167..2497
Translation	MSSNRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFKPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAAQNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQFCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILVQVLQILLKSKLLVLEDENANVDDVDLKPDILIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

Target ORF information:

RefSeq Version	NM_001045790.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cullin 1 (cul1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045790.1

ORF Insert Sequence:

ATGTCTTCAA ACAGAAGTCA GAATCCACAT GGACTGAAAC AGATTGGCTT GGACCAAATA 
TGGGATGACC TGCGGGCCGG GATCCAACAA GTTTATACAA GACAGAGCAT GGCAAAGTCC 
AGATATATGG AACTCTATAC ACATGTTTAT AACTACTGCA CCAGTGTACA CCAATCTAAC 
CAAGCACGGG GGGCTGGAGT GCCGCCATCA AAATCTAAGA AAGGACAGAC TCCTGGAGGT 
GCCCAGTTTG TTGGTTTGGA GCTCTACAAA AGACTAAAGG AGTTCTTAAA GAATTACTTG 
ACAAATCTCC TAAAGGATGG AGAAGATTTG ATGGATGAGA GCGTACTGAA GTTTTACACA 
CAGCAATGGG AAGATTACAG GTTTTCAAGT AAAGTGCTGA ATGGAATCTG CGCATACCTC 
AACCGGCACT GGGTTCGCCG TGAGTGTGAT GAAGGCCGCA AAGGAATATA TGAAATATAT 
TCTCTGGCCT TAGTGACATG GAGGGATTGT CTGTTTAAGC CTTTGAATAA GCAGGTAACA 
AATGCTGTGC TGAAACTGAT TGAAAAGGAA CGAAATGGTG AAACAATAAA CACCAGGCTT 
ATAAGTGGAG TGGTGCAATC TTATGTTGAG CTTGGCTTGA ATGAAGATGA TGCATTTGCC 
AAAGGCCCAA CACTGACTGT TTATAAAGAG TCCTTTGAGT CTCAGTTTTT GGCAGACACA 
GAACGGTTTT ATACAAGAGA GAGCACAGAG TTTTTACAGC AGAACCCTGT CACAGAATAT 
ATGAAAAAGG CAGAAGCTCG GCTTTTGGAG GAGCAGCGCA GAGTACAAGT ATACTTACAT 
GAGAGCACAC AGGATGAACT AGCACGGAAA TGTGAACAAG TCCTAATTGA AAAACATCTA 
GAGATTTTCC ACACTGAATT TCAGAATTTA CTAGATGCTG ATAAAAATGA AGACCTGGGC 
CGTATGTACA ATCTTGTTTC CCGAATCCAG GATGGTCTGG GAGAGCTGAA AAAGCTTTTA 
GAAACTCATA TTCACAATCA GGGACTTGCT GCCATAGAAA AATGTGGAGA AGCTGCACAA 
AATGATCCTA AAATGTATGT ACAGACTGTT TTGGATGTTC ACAAGAAATA CAATGCCTTA 
GTCATGTCAG CATTCAACAA TGATGCTGGA TTTGTGGCTG CACTAGATAA GGCTTGTGGT 
CGGTTTATAA ATAACAATGC TGTGACAAAA ATGGCTCAGT CTTCTAGCAA GTCACCAGAG 
TTGCTTGCCC GGTATTGTGA CTCTCTCTTG AAGAAGAGTT CCAAGAATCC AGAAGAAGCA 
GAGTTGGAGG ATACCCTTAA CCAAGTGATG GTGGTCTTTA AATACATAGA AGACAAGGAT 
GTGTTTCAGA AGTTCTATGC AAAAATGCTT GCAAAGCGAT TGGTTCATCA AAACAGTGCC 
AGTGATGATG CTGAAGCAAG TATGATATCA AAACTTAAGC AAGCCTGTGG GTTTGAGTAT 
ACATCAAAGC TTCAGAGAAT GTTTCAAGAT ATAGGTGTGA GCAAAGACCT TAATGAGCAG 
TTCAAAAAGC ACTTGACTAA TTCTGAGCCT TTAGACTTGG ACTTCAGCAT ACAGGTTCTC 
AGCTCTGGGT CATGGCCCTT CCAACAGTTC TGTACATTTG CTTTACCTTC AGAGTTGGAA 
CGCAGCTATC AAAGGTTTAC AGCTTTTTAT GCCAGCCGCC ACAGTGGAAG GAAGTTGACT 
TGGTTATACC AGTTGTCTAA AGGAGAACTG GTCACAAACT GCTTTAAAAA TAGATACACG 
TTGCAGGCAT CCACATTCCA GATGGCAATC CTGCTGCAGT ATAACACAGA AGATGCCTAC 
ACAGTGCAGC AACTGACAGA TAGCACACAG ATAAAAATGG ATATACTTGT GCAAGTTCTT 
CAAATTTTGC TGAAGTCTAA GTTATTGGTT TTAGAAGATG AAAATGCAAA TGTGGATGAT 
GTAGACTTGA AGCCAGATAT TTTAATAAAG CTCTACCTTG GTTATAAGAA TAAGAAGTTA 
AGAGTGAACA TAAATGTGCC CATGAAAACA GAACAGAAGC AGGAACAAGA AACAACACAC 
AAAAATATAG AGGAAGACCG AAAACTACTC ATTCAGGCAG CCATTGTGAG AATAATGAAG 
ATGAGGAAGG TTCTGAAACA CCAGCAGCTT CTTGGAGAAG TGTTGACTCA GCTGTCCTCC 
AGATTCAAAC CACGGGTCCC TGTTATCAAG AAATGCATTG ATATCCTAAT AGAAAAAGAG 
TATTTGGAGC GCGTGGATGG TGAAAAGGAC ACATACAGTT ACCTGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cul1 ( XM_012964856.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820310.1 Xenopus tropicalis cullin 1 (cul1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa28859
Clone ID Related Accession (Same CDS sequence)	XM_012964856.2 , XM_012964857.2 , XM_012964854.2
Accession Version	XM_012964856.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cullin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCAA ACAGAAGTCA GAATCCACAT GGACTGAAAC AGATTGGCTT GGACCAAATA 
TGGGATGACC TGCGGGCCGG GATCCAACAA GTTTATACAA GACAGAGCAT GGCAAAGTCC 
AGATATATGG AACTCTATAC ACATGTTTAT AACTACTGCA CCAGTGTACA CCAATCTAAC 
CAAGCACGGG GGGCTGGAGT GCCGCCATCA AAATCTAAGA AAGGACAGAC TCCTGGAGGT 
GCCCAGTTTG TTGGTTTGGA GCTCTACAAA AGACTAAAGG AGTTCTTAAA GAATTACTTG 
ACAAATCTCC TAAAGGATGG AGAAGATTTG ATGGATGAGA GCGTACTGAA GTTTTACACA 
CAGCAATGGG AAGATTACAG GTTTTCAAGT AAAGTGCTGA ATGGAATCTG TGCATACCTC 
AACCGGCACT GGGTTCGCCG TGAGTGTGAT GAAGGCCGCA AAGGAATATA TGAAATATAT 
TCTCTGGCCT TAGTGACATG GAGGGATTGT CTATTTAAGC CTTTGAATAA GCAGGTAACA 
AATGCTGTGC TGAAACTGAT TGAAAAGGAA CGAAATGGTG AAACAATAAA CACCAGGCTT 
ATAAGTGGAG TGGTGCAATC TTATGTTGAG CTTGGCTTGA ATGAAGATGA TGCATTTGCC 
AAAGGCCCAA CACTGACTGT TTATAAAGAG TCCTTTGAGT CTCAGTTTTT GGCAGACACA 
GAACGGTTTT ATACAAGAGA GAGCACAGAG TTTTTACAGC AGAACCCTGT CACAGAATAT 
ATGAAAAAGG CAGAAGCTCG GCTTTTGGAG GAGCAGCGCA GAGTACAAGT ATACTTACAT 
GAGAGCACAC AGGATGAACT AGCACGGAAA TGTGAACAAG TCCTAATTGA AAAACATCTA 
GAGATTTTCC ACACTGAATT TCAGAATTTA CTAGATGCTG ATAAAAATGA AGACCTGGGC 
CGTATGTACA ATCTTGTTTC CCGAATCCAG GATGGTCTGG GAGAGCTGAA AAAGCTTTTA 
GAAACTCATA TTCACAATCA GGGACTTGCT GCCATAGAAA AATGTGGAGA AGCTGCACAA 
AATGATCCTA AAATGTATGT ACAGACTGTT TTGGATGTTC ACAAGAAATA CAATGCCTTA 
GTCATGTCAG CATTCAACAA TGATGCTGGA TTTGTGGCTG CACTAGATAA GGCTTGTGGT 
CGGTTTATAA ATAACAATGC TGTGACAAAA ATGGCTCAGT CTTCTAGCAA GTCACCAGAG 
TTGCTTGCCC GGTATTGTGA CTCTCTCTTG AAGAAGAGTT CCAAGAATCC AGAAGAAGCA 
GAGTTGGAGG ATACCCTTAA CCAAGTGATG GTGGTCTTTA AATACATAGA AGACAAGGAT 
GTGTTTCAGA AGTTCTATGC AAAAATGCTT GCAAAGCGAT TGGTTCATCA AAACAGTGCC 
AGTGATGATG CTGAAGCAAG TATGATATCA AAACTTAAGC AAGCTTGTGG GTTTGAGTAT 
ACATCAAAGC TTCAGAGAAT GTTTCAAGAT ATAGGTGTGA GCAAAGACCT TAATGAGCAG 
TTCAAAAAGC ACTTGACTAA TTCTGAGCCT TTAGACTTGG ACTTCAGCAT ACAGGTTCTC 
AGCTCTGGGT CATGGCCCTT CCAACAGTTC TGTACATTTG CTTTACCTTC AGAGTTGGAA 
CGCAGCTATC AAAGGTTTAC AGCTTTTTAT GCCAGCCGCC ACAGTGGAAG GAAGTTGACT 
TGGTTATACC AGTTGTCTAA AGGAGAACTG GTCACAAACT GCTTTAAAAA TAGATACACG 
TTGCAGGCAT CCACATTCCA GATGGCAATC CTGCTGCAGT ATAACACAGA AGATGCCTAC 
ACAGTGCAGC AACTGACAGA TAGCACACAG ATAAAAATGG ATATACTTGT GCAAGTTCTT 
CAAATTTTGC TGAAGTCTAA GTTATTGGTT TTAGAAGATG AAAATGCAAA TGTGGATGAT 
GTAGACTTGA AGCCAGATAT TTTAATAAAG CTCTACCTTG GTTATAAGAA TAAGAAGTTA 
AGAGTGAACA TAAATGTGCC CATGAAAACA GAACAGAAGC AGGAACAAGA AACAACACAC 
AAAAATATAG AGGAAGACCG AAAACTACTC ATTCAGGCAG CCATTGTGAG AATAATGAAG 
ATGAGGAAGG TTCTGAAACA CCAGCAGCTT CTTGGAGAAG TGTTGACTCA GCTGTCCTCC 
AGATTCAAAC CACGGGTCCC TGTTATCAAG AAATGCATTG ATATCCTAAT AGAAAAAGAG 
TATTTGGAGC GCGTGGATGG TGAAAAGGAC ACATACAGTT ACCTGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820310.1
CDS	292..2622
Translation	MSSNRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFKPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAAQNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQFCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILVQVLQILLKSKLLVLEDENANVDDVDLKPDILIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

Target ORF information:

RefSeq Version	XM_012964856.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cullin 1 (cul1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964856.2

ORF Insert Sequence:

ATGTCTTCAA ACAGAAGTCA GAATCCACAT GGACTGAAAC AGATTGGCTT GGACCAAATA 
TGGGATGACC TGCGGGCCGG GATCCAACAA GTTTATACAA GACAGAGCAT GGCAAAGTCC 
AGATATATGG AACTCTATAC ACATGTTTAT AACTACTGCA CCAGTGTACA CCAATCTAAC 
CAAGCACGGG GGGCTGGAGT GCCGCCATCA AAATCTAAGA AAGGACAGAC TCCTGGAGGT 
GCCCAGTTTG TTGGTTTGGA GCTCTACAAA AGACTAAAGG AGTTCTTAAA GAATTACTTG 
ACAAATCTCC TAAAGGATGG AGAAGATTTG ATGGATGAGA GCGTACTGAA GTTTTACACA 
CAGCAATGGG AAGATTACAG GTTTTCAAGT AAAGTGCTGA ATGGAATCTG TGCATACCTC 
AACCGGCACT GGGTTCGCCG TGAGTGTGAT GAAGGCCGCA AAGGAATATA TGAAATATAT 
TCTCTGGCCT TAGTGACATG GAGGGATTGT CTATTTAAGC CTTTGAATAA GCAGGTAACA 
AATGCTGTGC TGAAACTGAT TGAAAAGGAA CGAAATGGTG AAACAATAAA CACCAGGCTT 
ATAAGTGGAG TGGTGCAATC TTATGTTGAG CTTGGCTTGA ATGAAGATGA TGCATTTGCC 
AAAGGCCCAA CACTGACTGT TTATAAAGAG TCCTTTGAGT CTCAGTTTTT GGCAGACACA 
GAACGGTTTT ATACAAGAGA GAGCACAGAG TTTTTACAGC AGAACCCTGT CACAGAATAT 
ATGAAAAAGG CAGAAGCTCG GCTTTTGGAG GAGCAGCGCA GAGTACAAGT ATACTTACAT 
GAGAGCACAC AGGATGAACT AGCACGGAAA TGTGAACAAG TCCTAATTGA AAAACATCTA 
GAGATTTTCC ACACTGAATT TCAGAATTTA CTAGATGCTG ATAAAAATGA AGACCTGGGC 
CGTATGTACA ATCTTGTTTC CCGAATCCAG GATGGTCTGG GAGAGCTGAA AAAGCTTTTA 
GAAACTCATA TTCACAATCA GGGACTTGCT GCCATAGAAA AATGTGGAGA AGCTGCACAA 
AATGATCCTA AAATGTATGT ACAGACTGTT TTGGATGTTC ACAAGAAATA CAATGCCTTA 
GTCATGTCAG CATTCAACAA TGATGCTGGA TTTGTGGCTG CACTAGATAA GGCTTGTGGT 
CGGTTTATAA ATAACAATGC TGTGACAAAA ATGGCTCAGT CTTCTAGCAA GTCACCAGAG 
TTGCTTGCCC GGTATTGTGA CTCTCTCTTG AAGAAGAGTT CCAAGAATCC AGAAGAAGCA 
GAGTTGGAGG ATACCCTTAA CCAAGTGATG GTGGTCTTTA AATACATAGA AGACAAGGAT 
GTGTTTCAGA AGTTCTATGC AAAAATGCTT GCAAAGCGAT TGGTTCATCA AAACAGTGCC 
AGTGATGATG CTGAAGCAAG TATGATATCA AAACTTAAGC AAGCTTGTGG GTTTGAGTAT 
ACATCAAAGC TTCAGAGAAT GTTTCAAGAT ATAGGTGTGA GCAAAGACCT TAATGAGCAG 
TTCAAAAAGC ACTTGACTAA TTCTGAGCCT TTAGACTTGG ACTTCAGCAT ACAGGTTCTC 
AGCTCTGGGT CATGGCCCTT CCAACAGTTC TGTACATTTG CTTTACCTTC AGAGTTGGAA 
CGCAGCTATC AAAGGTTTAC AGCTTTTTAT GCCAGCCGCC ACAGTGGAAG GAAGTTGACT 
TGGTTATACC AGTTGTCTAA AGGAGAACTG GTCACAAACT GCTTTAAAAA TAGATACACG 
TTGCAGGCAT CCACATTCCA GATGGCAATC CTGCTGCAGT ATAACACAGA AGATGCCTAC 
ACAGTGCAGC AACTGACAGA TAGCACACAG ATAAAAATGG ATATACTTGT GCAAGTTCTT 
CAAATTTTGC TGAAGTCTAA GTTATTGGTT TTAGAAGATG AAAATGCAAA TGTGGATGAT 
GTAGACTTGA AGCCAGATAT TTTAATAAAG CTCTACCTTG GTTATAAGAA TAAGAAGTTA 
AGAGTGAACA TAAATGTGCC CATGAAAACA GAACAGAAGC AGGAACAAGA AACAACACAC 
AAAAATATAG AGGAAGACCG AAAACTACTC ATTCAGGCAG CCATTGTGAG AATAATGAAG 
ATGAGGAAGG TTCTGAAACA CCAGCAGCTT CTTGGAGAAG TGTTGACTCA GCTGTCCTCC 
AGATTCAAAC CACGGGTCCC TGTTATCAAG AAATGCATTG ATATCCTAAT AGAAAAAGAG 
TATTTGGAGC GCGTGGATGG TGAAAAGGAC ACATACAGTT ACCTGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cul1 ( XM_012964857.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820311.1 Xenopus tropicalis cullin 1 (cul1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa28859
Clone ID Related Accession (Same CDS sequence)	XM_012964856.2 , XM_012964857.2 , XM_012964854.2
Accession Version	XM_012964857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cullin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCAA ACAGAAGTCA GAATCCACAT GGACTGAAAC AGATTGGCTT GGACCAAATA 
TGGGATGACC TGCGGGCCGG GATCCAACAA GTTTATACAA GACAGAGCAT GGCAAAGTCC 
AGATATATGG AACTCTATAC ACATGTTTAT AACTACTGCA CCAGTGTACA CCAATCTAAC 
CAAGCACGGG GGGCTGGAGT GCCGCCATCA AAATCTAAGA AAGGACAGAC TCCTGGAGGT 
GCCCAGTTTG TTGGTTTGGA GCTCTACAAA AGACTAAAGG AGTTCTTAAA GAATTACTTG 
ACAAATCTCC TAAAGGATGG AGAAGATTTG ATGGATGAGA GCGTACTGAA GTTTTACACA 
CAGCAATGGG AAGATTACAG GTTTTCAAGT AAAGTGCTGA ATGGAATCTG TGCATACCTC 
AACCGGCACT GGGTTCGCCG TGAGTGTGAT GAAGGCCGCA AAGGAATATA TGAAATATAT 
TCTCTGGCCT TAGTGACATG GAGGGATTGT CTATTTAAGC CTTTGAATAA GCAGGTAACA 
AATGCTGTGC TGAAACTGAT TGAAAAGGAA CGAAATGGTG AAACAATAAA CACCAGGCTT 
ATAAGTGGAG TGGTGCAATC TTATGTTGAG CTTGGCTTGA ATGAAGATGA TGCATTTGCC 
AAAGGCCCAA CACTGACTGT TTATAAAGAG TCCTTTGAGT CTCAGTTTTT GGCAGACACA 
GAACGGTTTT ATACAAGAGA GAGCACAGAG TTTTTACAGC AGAACCCTGT CACAGAATAT 
ATGAAAAAGG CAGAAGCTCG GCTTTTGGAG GAGCAGCGCA GAGTACAAGT ATACTTACAT 
GAGAGCACAC AGGATGAACT AGCACGGAAA TGTGAACAAG TCCTAATTGA AAAACATCTA 
GAGATTTTCC ACACTGAATT TCAGAATTTA CTAGATGCTG ATAAAAATGA AGACCTGGGC 
CGTATGTACA ATCTTGTTTC CCGAATCCAG GATGGTCTGG GAGAGCTGAA AAAGCTTTTA 
GAAACTCATA TTCACAATCA GGGACTTGCT GCCATAGAAA AATGTGGAGA AGCTGCACAA 
AATGATCCTA AAATGTATGT ACAGACTGTT TTGGATGTTC ACAAGAAATA CAATGCCTTA 
GTCATGTCAG CATTCAACAA TGATGCTGGA TTTGTGGCTG CACTAGATAA GGCTTGTGGT 
CGGTTTATAA ATAACAATGC TGTGACAAAA ATGGCTCAGT CTTCTAGCAA GTCACCAGAG 
TTGCTTGCCC GGTATTGTGA CTCTCTCTTG AAGAAGAGTT CCAAGAATCC AGAAGAAGCA 
GAGTTGGAGG ATACCCTTAA CCAAGTGATG GTGGTCTTTA AATACATAGA AGACAAGGAT 
GTGTTTCAGA AGTTCTATGC AAAAATGCTT GCAAAGCGAT TGGTTCATCA AAACAGTGCC 
AGTGATGATG CTGAAGCAAG TATGATATCA AAACTTAAGC AAGCTTGTGG GTTTGAGTAT 
ACATCAAAGC TTCAGAGAAT GTTTCAAGAT ATAGGTGTGA GCAAAGACCT TAATGAGCAG 
TTCAAAAAGC ACTTGACTAA TTCTGAGCCT TTAGACTTGG ACTTCAGCAT ACAGGTTCTC 
AGCTCTGGGT CATGGCCCTT CCAACAGTTC TGTACATTTG CTTTACCTTC AGAGTTGGAA 
CGCAGCTATC AAAGGTTTAC AGCTTTTTAT GCCAGCCGCC ACAGTGGAAG GAAGTTGACT 
TGGTTATACC AGTTGTCTAA AGGAGAACTG GTCACAAACT GCTTTAAAAA TAGATACACG 
TTGCAGGCAT CCACATTCCA GATGGCAATC CTGCTGCAGT ATAACACAGA AGATGCCTAC 
ACAGTGCAGC AACTGACAGA TAGCACACAG ATAAAAATGG ATATACTTGT GCAAGTTCTT 
CAAATTTTGC TGAAGTCTAA GTTATTGGTT TTAGAAGATG AAAATGCAAA TGTGGATGAT 
GTAGACTTGA AGCCAGATAT TTTAATAAAG CTCTACCTTG GTTATAAGAA TAAGAAGTTA 
AGAGTGAACA TAAATGTGCC CATGAAAACA GAACAGAAGC AGGAACAAGA AACAACACAC 
AAAAATATAG AGGAAGACCG AAAACTACTC ATTCAGGCAG CCATTGTGAG AATAATGAAG 
ATGAGGAAGG TTCTGAAACA CCAGCAGCTT CTTGGAGAAG TGTTGACTCA GCTGTCCTCC 
AGATTCAAAC CACGGGTCCC TGTTATCAAG AAATGCATTG ATATCCTAAT AGAAAAAGAG 
TATTTGGAGC GCGTGGATGG TGAAAAGGAC ACATACAGTT ACCTGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820311.1
CDS	186..2516
Translation	MSSNRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFKPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAAQNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQFCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILVQVLQILLKSKLLVLEDENANVDDVDLKPDILIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

Target ORF information:

RefSeq Version	XM_012964857.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cullin 1 (cul1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964857.2

ORF Insert Sequence:

ATGTCTTCAA ACAGAAGTCA GAATCCACAT GGACTGAAAC AGATTGGCTT GGACCAAATA 
TGGGATGACC TGCGGGCCGG GATCCAACAA GTTTATACAA GACAGAGCAT GGCAAAGTCC 
AGATATATGG AACTCTATAC ACATGTTTAT AACTACTGCA CCAGTGTACA CCAATCTAAC 
CAAGCACGGG GGGCTGGAGT GCCGCCATCA AAATCTAAGA AAGGACAGAC TCCTGGAGGT 
GCCCAGTTTG TTGGTTTGGA GCTCTACAAA AGACTAAAGG AGTTCTTAAA GAATTACTTG 
ACAAATCTCC TAAAGGATGG AGAAGATTTG ATGGATGAGA GCGTACTGAA GTTTTACACA 
CAGCAATGGG AAGATTACAG GTTTTCAAGT AAAGTGCTGA ATGGAATCTG TGCATACCTC 
AACCGGCACT GGGTTCGCCG TGAGTGTGAT GAAGGCCGCA AAGGAATATA TGAAATATAT 
TCTCTGGCCT TAGTGACATG GAGGGATTGT CTATTTAAGC CTTTGAATAA GCAGGTAACA 
AATGCTGTGC TGAAACTGAT TGAAAAGGAA CGAAATGGTG AAACAATAAA CACCAGGCTT 
ATAAGTGGAG TGGTGCAATC TTATGTTGAG CTTGGCTTGA ATGAAGATGA TGCATTTGCC 
AAAGGCCCAA CACTGACTGT TTATAAAGAG TCCTTTGAGT CTCAGTTTTT GGCAGACACA 
GAACGGTTTT ATACAAGAGA GAGCACAGAG TTTTTACAGC AGAACCCTGT CACAGAATAT 
ATGAAAAAGG CAGAAGCTCG GCTTTTGGAG GAGCAGCGCA GAGTACAAGT ATACTTACAT 
GAGAGCACAC AGGATGAACT AGCACGGAAA TGTGAACAAG TCCTAATTGA AAAACATCTA 
GAGATTTTCC ACACTGAATT TCAGAATTTA CTAGATGCTG ATAAAAATGA AGACCTGGGC 
CGTATGTACA ATCTTGTTTC CCGAATCCAG GATGGTCTGG GAGAGCTGAA AAAGCTTTTA 
GAAACTCATA TTCACAATCA GGGACTTGCT GCCATAGAAA AATGTGGAGA AGCTGCACAA 
AATGATCCTA AAATGTATGT ACAGACTGTT TTGGATGTTC ACAAGAAATA CAATGCCTTA 
GTCATGTCAG CATTCAACAA TGATGCTGGA TTTGTGGCTG CACTAGATAA GGCTTGTGGT 
CGGTTTATAA ATAACAATGC TGTGACAAAA ATGGCTCAGT CTTCTAGCAA GTCACCAGAG 
TTGCTTGCCC GGTATTGTGA CTCTCTCTTG AAGAAGAGTT CCAAGAATCC AGAAGAAGCA 
GAGTTGGAGG ATACCCTTAA CCAAGTGATG GTGGTCTTTA AATACATAGA AGACAAGGAT 
GTGTTTCAGA AGTTCTATGC AAAAATGCTT GCAAAGCGAT TGGTTCATCA AAACAGTGCC 
AGTGATGATG CTGAAGCAAG TATGATATCA AAACTTAAGC AAGCTTGTGG GTTTGAGTAT 
ACATCAAAGC TTCAGAGAAT GTTTCAAGAT ATAGGTGTGA GCAAAGACCT TAATGAGCAG 
TTCAAAAAGC ACTTGACTAA TTCTGAGCCT TTAGACTTGG ACTTCAGCAT ACAGGTTCTC 
AGCTCTGGGT CATGGCCCTT CCAACAGTTC TGTACATTTG CTTTACCTTC AGAGTTGGAA 
CGCAGCTATC AAAGGTTTAC AGCTTTTTAT GCCAGCCGCC ACAGTGGAAG GAAGTTGACT 
TGGTTATACC AGTTGTCTAA AGGAGAACTG GTCACAAACT GCTTTAAAAA TAGATACACG 
TTGCAGGCAT CCACATTCCA GATGGCAATC CTGCTGCAGT ATAACACAGA AGATGCCTAC 
ACAGTGCAGC AACTGACAGA TAGCACACAG ATAAAAATGG ATATACTTGT GCAAGTTCTT 
CAAATTTTGC TGAAGTCTAA GTTATTGGTT TTAGAAGATG AAAATGCAAA TGTGGATGAT 
GTAGACTTGA AGCCAGATAT TTTAATAAAG CTCTACCTTG GTTATAAGAA TAAGAAGTTA 
AGAGTGAACA TAAATGTGCC CATGAAAACA GAACAGAAGC AGGAACAAGA AACAACACAC 
AAAAATATAG AGGAAGACCG AAAACTACTC ATTCAGGCAG CCATTGTGAG AATAATGAAG 
ATGAGGAAGG TTCTGAAACA CCAGCAGCTT CTTGGAGAAG TGTTGACTCA GCTGTCCTCC 
AGATTCAAAC CACGGGTCCC TGTTATCAAG AAATGCATTG ATATCCTAAT AGAAAAAGAG 
TATTTGGAGC GCGTGGATGG TGAAAAGGAC ACATACAGTT ACCTGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cul1 ( XM_012964854.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820308.1 Xenopus tropicalis cullin 1 (cul1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa28859
Clone ID Related Accession (Same CDS sequence)	XM_012964856.2 , XM_012964857.2 , XM_012964854.2
Accession Version	XM_012964854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cullin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCAA ACAGAAGTCA GAATCCACAT GGACTGAAAC AGATTGGCTT GGACCAAATA 
TGGGATGACC TGCGGGCCGG GATCCAACAA GTTTATACAA GACAGAGCAT GGCAAAGTCC 
AGATATATGG AACTCTATAC ACATGTTTAT AACTACTGCA CCAGTGTACA CCAATCTAAC 
CAAGCACGGG GGGCTGGAGT GCCGCCATCA AAATCTAAGA AAGGACAGAC TCCTGGAGGT 
GCCCAGTTTG TTGGTTTGGA GCTCTACAAA AGACTAAAGG AGTTCTTAAA GAATTACTTG 
ACAAATCTCC TAAAGGATGG AGAAGATTTG ATGGATGAGA GCGTACTGAA GTTTTACACA 
CAGCAATGGG AAGATTACAG GTTTTCAAGT AAAGTGCTGA ATGGAATCTG TGCATACCTC 
AACCGGCACT GGGTTCGCCG TGAGTGTGAT GAAGGCCGCA AAGGAATATA TGAAATATAT 
TCTCTGGCCT TAGTGACATG GAGGGATTGT CTATTTAAGC CTTTGAATAA GCAGGTAACA 
AATGCTGTGC TGAAACTGAT TGAAAAGGAA CGAAATGGTG AAACAATAAA CACCAGGCTT 
ATAAGTGGAG TGGTGCAATC TTATGTTGAG CTTGGCTTGA ATGAAGATGA TGCATTTGCC 
AAAGGCCCAA CACTGACTGT TTATAAAGAG TCCTTTGAGT CTCAGTTTTT GGCAGACACA 
GAACGGTTTT ATACAAGAGA GAGCACAGAG TTTTTACAGC AGAACCCTGT CACAGAATAT 
ATGAAAAAGG CAGAAGCTCG GCTTTTGGAG GAGCAGCGCA GAGTACAAGT ATACTTACAT 
GAGAGCACAC AGGATGAACT AGCACGGAAA TGTGAACAAG TCCTAATTGA AAAACATCTA 
GAGATTTTCC ACACTGAATT TCAGAATTTA CTAGATGCTG ATAAAAATGA AGACCTGGGC 
CGTATGTACA ATCTTGTTTC CCGAATCCAG GATGGTCTGG GAGAGCTGAA AAAGCTTTTA 
GAAACTCATA TTCACAATCA GGGACTTGCT GCCATAGAAA AATGTGGAGA AGCTGCACAA 
AATGATCCTA AAATGTATGT ACAGACTGTT TTGGATGTTC ACAAGAAATA CAATGCCTTA 
GTCATGTCAG CATTCAACAA TGATGCTGGA TTTGTGGCTG CACTAGATAA GGCTTGTGGT 
CGGTTTATAA ATAACAATGC TGTGACAAAA ATGGCTCAGT CTTCTAGCAA GTCACCAGAG 
TTGCTTGCCC GGTATTGTGA CTCTCTCTTG AAGAAGAGTT CCAAGAATCC AGAAGAAGCA 
GAGTTGGAGG ATACCCTTAA CCAAGTGATG GTGGTCTTTA AATACATAGA AGACAAGGAT 
GTGTTTCAGA AGTTCTATGC AAAAATGCTT GCAAAGCGAT TGGTTCATCA AAACAGTGCC 
AGTGATGATG CTGAAGCAAG TATGATATCA AAACTTAAGC AAGCTTGTGG GTTTGAGTAT 
ACATCAAAGC TTCAGAGAAT GTTTCAAGAT ATAGGTGTGA GCAAAGACCT TAATGAGCAG 
TTCAAAAAGC ACTTGACTAA TTCTGAGCCT TTAGACTTGG ACTTCAGCAT ACAGGTTCTC 
AGCTCTGGGT CATGGCCCTT CCAACAGTTC TGTACATTTG CTTTACCTTC AGAGTTGGAA 
CGCAGCTATC AAAGGTTTAC AGCTTTTTAT GCCAGCCGCC ACAGTGGAAG GAAGTTGACT 
TGGTTATACC AGTTGTCTAA AGGAGAACTG GTCACAAACT GCTTTAAAAA TAGATACACG 
TTGCAGGCAT CCACATTCCA GATGGCAATC CTGCTGCAGT ATAACACAGA AGATGCCTAC 
ACAGTGCAGC AACTGACAGA TAGCACACAG ATAAAAATGG ATATACTTGT GCAAGTTCTT 
CAAATTTTGC TGAAGTCTAA GTTATTGGTT TTAGAAGATG AAAATGCAAA TGTGGATGAT 
GTAGACTTGA AGCCAGATAT TTTAATAAAG CTCTACCTTG GTTATAAGAA TAAGAAGTTA 
AGAGTGAACA TAAATGTGCC CATGAAAACA GAACAGAAGC AGGAACAAGA AACAACACAC 
AAAAATATAG AGGAAGACCG AAAACTACTC ATTCAGGCAG CCATTGTGAG AATAATGAAG 
ATGAGGAAGG TTCTGAAACA CCAGCAGCTT CTTGGAGAAG TGTTGACTCA GCTGTCCTCC 
AGATTCAAAC CACGGGTCCC TGTTATCAAG AAATGCATTG ATATCCTAAT AGAAAAAGAG 
TATTTGGAGC GCGTGGATGG TGAAAAGGAC ACATACAGTT ACCTGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820308.1
CDS	659..2989
Translation	MSSNRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFKPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAAQNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQFCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILVQVLQILLKSKLLVLEDENANVDDVDLKPDILIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

Target ORF information:

RefSeq Version	XM_012964854.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cullin 1 (cul1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964854.2

ORF Insert Sequence:

ATGTCTTCAA ACAGAAGTCA GAATCCACAT GGACTGAAAC AGATTGGCTT GGACCAAATA 
TGGGATGACC TGCGGGCCGG GATCCAACAA GTTTATACAA GACAGAGCAT GGCAAAGTCC 
AGATATATGG AACTCTATAC ACATGTTTAT AACTACTGCA CCAGTGTACA CCAATCTAAC 
CAAGCACGGG GGGCTGGAGT GCCGCCATCA AAATCTAAGA AAGGACAGAC TCCTGGAGGT 
GCCCAGTTTG TTGGTTTGGA GCTCTACAAA AGACTAAAGG AGTTCTTAAA GAATTACTTG 
ACAAATCTCC TAAAGGATGG AGAAGATTTG ATGGATGAGA GCGTACTGAA GTTTTACACA 
CAGCAATGGG AAGATTACAG GTTTTCAAGT AAAGTGCTGA ATGGAATCTG TGCATACCTC 
AACCGGCACT GGGTTCGCCG TGAGTGTGAT GAAGGCCGCA AAGGAATATA TGAAATATAT 
TCTCTGGCCT TAGTGACATG GAGGGATTGT CTATTTAAGC CTTTGAATAA GCAGGTAACA 
AATGCTGTGC TGAAACTGAT TGAAAAGGAA CGAAATGGTG AAACAATAAA CACCAGGCTT 
ATAAGTGGAG TGGTGCAATC TTATGTTGAG CTTGGCTTGA ATGAAGATGA TGCATTTGCC 
AAAGGCCCAA CACTGACTGT TTATAAAGAG TCCTTTGAGT CTCAGTTTTT GGCAGACACA 
GAACGGTTTT ATACAAGAGA GAGCACAGAG TTTTTACAGC AGAACCCTGT CACAGAATAT 
ATGAAAAAGG CAGAAGCTCG GCTTTTGGAG GAGCAGCGCA GAGTACAAGT ATACTTACAT 
GAGAGCACAC AGGATGAACT AGCACGGAAA TGTGAACAAG TCCTAATTGA AAAACATCTA 
GAGATTTTCC ACACTGAATT TCAGAATTTA CTAGATGCTG ATAAAAATGA AGACCTGGGC 
CGTATGTACA ATCTTGTTTC CCGAATCCAG GATGGTCTGG GAGAGCTGAA AAAGCTTTTA 
GAAACTCATA TTCACAATCA GGGACTTGCT GCCATAGAAA AATGTGGAGA AGCTGCACAA 
AATGATCCTA AAATGTATGT ACAGACTGTT TTGGATGTTC ACAAGAAATA CAATGCCTTA 
GTCATGTCAG CATTCAACAA TGATGCTGGA TTTGTGGCTG CACTAGATAA GGCTTGTGGT 
CGGTTTATAA ATAACAATGC TGTGACAAAA ATGGCTCAGT CTTCTAGCAA GTCACCAGAG 
TTGCTTGCCC GGTATTGTGA CTCTCTCTTG AAGAAGAGTT CCAAGAATCC AGAAGAAGCA 
GAGTTGGAGG ATACCCTTAA CCAAGTGATG GTGGTCTTTA AATACATAGA AGACAAGGAT 
GTGTTTCAGA AGTTCTATGC AAAAATGCTT GCAAAGCGAT TGGTTCATCA AAACAGTGCC 
AGTGATGATG CTGAAGCAAG TATGATATCA AAACTTAAGC AAGCTTGTGG GTTTGAGTAT 
ACATCAAAGC TTCAGAGAAT GTTTCAAGAT ATAGGTGTGA GCAAAGACCT TAATGAGCAG 
TTCAAAAAGC ACTTGACTAA TTCTGAGCCT TTAGACTTGG ACTTCAGCAT ACAGGTTCTC 
AGCTCTGGGT CATGGCCCTT CCAACAGTTC TGTACATTTG CTTTACCTTC AGAGTTGGAA 
CGCAGCTATC AAAGGTTTAC AGCTTTTTAT GCCAGCCGCC ACAGTGGAAG GAAGTTGACT 
TGGTTATACC AGTTGTCTAA AGGAGAACTG GTCACAAACT GCTTTAAAAA TAGATACACG 
TTGCAGGCAT CCACATTCCA GATGGCAATC CTGCTGCAGT ATAACACAGA AGATGCCTAC 
ACAGTGCAGC AACTGACAGA TAGCACACAG ATAAAAATGG ATATACTTGT GCAAGTTCTT 
CAAATTTTGC TGAAGTCTAA GTTATTGGTT TTAGAAGATG AAAATGCAAA TGTGGATGAT 
GTAGACTTGA AGCCAGATAT TTTAATAAAG CTCTACCTTG GTTATAAGAA TAAGAAGTTA 
AGAGTGAACA TAAATGTGCC CATGAAAACA GAACAGAAGC AGGAACAAGA AACAACACAC 
AAAAATATAG AGGAAGACCG AAAACTACTC ATTCAGGCAG CCATTGTGAG AATAATGAAG 
ATGAGGAAGG TTCTGAAACA CCAGCAGCTT CTTGGAGAAG TGTTGACTCA GCTGTCCTCC 
AGATTCAAAC CACGGGTCCC TGTTATCAAG AAATGCATTG ATATCCTAAT AGAAAAAGAG 
TATTTGGAGC GCGTGGATGG TGAAAAGGAC ACATACAGTT ACCTGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cul1 ( XM_012964854.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820308.1 Xenopus tropicalis cullin 1 (cul1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa25199
Clone ID Related Accession (Same CDS sequence)	XM_012964854.3 , XM_012964856.3 , NM_001045790.1
Accession Version	XM_012964854.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cullin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCAA ACAGAAGTCA GAATCCACAT GGACTGAAAC AGATTGGCTT GGACCAAATA 
TGGGATGACC TGCGGGCCGG GATCCAACAA GTTTATACAA GACAGAGCAT GGCAAAGTCC 
AGATATATGG AACTCTATAC ACATGTTTAT AACTACTGCA CCAGTGTACA CCAATCTAAC 
CAAGCACGGG GGGCTGGAGT GCCGCCATCA AAATCTAAGA AAGGACAGAC TCCTGGAGGT 
GCCCAGTTTG TTGGTTTGGA GCTCTACAAA AGACTAAAGG AGTTCTTAAA GAATTACTTG 
ACAAATCTCC TAAAGGATGG AGAAGATTTG ATGGATGAGA GCGTACTGAA GTTTTACACA 
CAGCAATGGG AAGATTACAG GTTTTCAAGT AAAGTGCTGA ATGGAATCTG CGCATACCTC 
AACCGGCACT GGGTTCGCCG TGAGTGTGAT GAAGGCCGCA AAGGAATATA TGAAATATAT 
TCTCTGGCCT TAGTGACATG GAGGGATTGT CTGTTTAAGC CTTTGAATAA GCAGGTAACA 
AATGCTGTGC TGAAACTGAT TGAAAAGGAA CGAAATGGTG AAACAATAAA CACCAGGCTT 
ATAAGTGGAG TGGTGCAATC TTATGTTGAG CTTGGCTTGA ATGAAGATGA TGCATTTGCC 
AAAGGCCCAA CACTGACTGT TTATAAAGAG TCCTTTGAGT CTCAGTTTTT GGCAGACACA 
GAACGGTTTT ATACAAGAGA GAGCACAGAG TTTTTACAGC AGAACCCTGT CACAGAATAT 
ATGAAAAAGG CAGAAGCTCG GCTTTTGGAG GAGCAGCGCA GAGTACAAGT ATACTTACAT 
GAGAGCACAC AGGATGAACT AGCACGGAAA TGTGAACAAG TCCTAATTGA AAAACATCTA 
GAGATTTTCC ACACTGAATT TCAGAATTTA CTAGATGCTG ATAAAAATGA AGACCTGGGC 
CGTATGTACA ATCTTGTTTC CCGAATCCAG GATGGTCTGG GAGAGCTGAA AAAGCTTTTA 
GAAACTCATA TTCACAATCA GGGACTTGCT GCCATAGAAA AATGTGGAGA AGCTGCACAA 
AATGATCCTA AAATGTATGT ACAGACTGTT TTGGATGTTC ACAAGAAATA CAATGCCTTA 
GTCATGTCAG CATTCAACAA TGATGCTGGA TTTGTGGCTG CACTAGATAA GGCTTGTGGT 
CGGTTTATAA ATAACAATGC TGTGACAAAA ATGGCTCAGT CTTCTAGCAA GTCACCAGAG 
TTGCTTGCCC GGTATTGTGA CTCTCTCTTG AAGAAGAGTT CCAAGAATCC AGAAGAAGCA 
GAGTTGGAGG ATACCCTTAA CCAAGTGATG GTGGTCTTTA AATACATAGA AGACAAGGAT 
GTGTTTCAGA AGTTCTATGC AAAAATGCTT GCAAAGCGAT TGGTTCATCA AAACAGTGCC 
AGTGATGATG CTGAAGCAAG TATGATATCA AAACTTAAGC AAGCCTGTGG GTTTGAGTAT 
ACATCAAAGC TTCAGAGAAT GTTTCAAGAT ATAGGTGTGA GCAAAGACCT TAATGAGCAG 
TTCAAAAAGC ACTTGACTAA TTCTGAGCCT TTAGACTTGG ACTTCAGCAT ACAGGTTCTC 
AGCTCTGGGT CATGGCCCTT CCAACAGTTC TGTACATTTG CTTTACCTTC AGAGTTGGAA 
CGCAGCTATC AAAGGTTTAC AGCTTTTTAT GCCAGCCGCC ACAGTGGAAG GAAGTTGACT 
TGGTTATACC AGTTGTCTAA AGGAGAACTG GTCACAAACT GCTTTAAAAA TAGATACACG 
TTGCAGGCAT CCACATTCCA GATGGCAATC CTGCTGCAGT ATAACACAGA AGATGCCTAC 
ACAGTGCAGC AACTGACAGA TAGCACACAG ATAAAAATGG ATATACTTGT GCAAGTTCTT 
CAAATTTTGC TGAAGTCTAA GTTATTGGTT TTAGAAGATG AAAATGCAAA TGTGGATGAT 
GTAGACTTGA AGCCAGATAT TTTAATAAAG CTCTACCTTG GTTATAAGAA TAAGAAGTTA 
AGAGTGAACA TAAATGTGCC CATGAAAACA GAACAGAAGC AGGAACAAGA AACAACACAC 
AAAAATATAG AGGAAGACCG AAAACTACTC ATTCAGGCAG CCATTGTGAG AATAATGAAG 
ATGAGGAAGG TTCTGAAACA CCAGCAGCTT CTTGGAGAAG TGTTGACTCA GCTGTCCTCC 
AGATTCAAAC CACGGGTCCC TGTTATCAAG AAATGCATTG ATATCCTAAT AGAAAAAGAG 
TATTTGGAGC GCGTGGATGG TGAAAAGGAC ACATACAGTT ACCTGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820308.1
CDS	659..2989
Translation	MSSNRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFKPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAAQNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQFCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILVQVLQILLKSKLLVLEDENANVDDVDLKPDILIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

Target ORF information:

RefSeq Version	XM_012964854.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cullin 1 (cul1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964854.3

ORF Insert Sequence:

ATGTCTTCAA ACAGAAGTCA GAATCCACAT GGACTGAAAC AGATTGGCTT GGACCAAATA 
TGGGATGACC TGCGGGCCGG GATCCAACAA GTTTATACAA GACAGAGCAT GGCAAAGTCC 
AGATATATGG AACTCTATAC ACATGTTTAT AACTACTGCA CCAGTGTACA CCAATCTAAC 
CAAGCACGGG GGGCTGGAGT GCCGCCATCA AAATCTAAGA AAGGACAGAC TCCTGGAGGT 
GCCCAGTTTG TTGGTTTGGA GCTCTACAAA AGACTAAAGG AGTTCTTAAA GAATTACTTG 
ACAAATCTCC TAAAGGATGG AGAAGATTTG ATGGATGAGA GCGTACTGAA GTTTTACACA 
CAGCAATGGG AAGATTACAG GTTTTCAAGT AAAGTGCTGA ATGGAATCTG CGCATACCTC 
AACCGGCACT GGGTTCGCCG TGAGTGTGAT GAAGGCCGCA AAGGAATATA TGAAATATAT 
TCTCTGGCCT TAGTGACATG GAGGGATTGT CTGTTTAAGC CTTTGAATAA GCAGGTAACA 
AATGCTGTGC TGAAACTGAT TGAAAAGGAA CGAAATGGTG AAACAATAAA CACCAGGCTT 
ATAAGTGGAG TGGTGCAATC TTATGTTGAG CTTGGCTTGA ATGAAGATGA TGCATTTGCC 
AAAGGCCCAA CACTGACTGT TTATAAAGAG TCCTTTGAGT CTCAGTTTTT GGCAGACACA 
GAACGGTTTT ATACAAGAGA GAGCACAGAG TTTTTACAGC AGAACCCTGT CACAGAATAT 
ATGAAAAAGG CAGAAGCTCG GCTTTTGGAG GAGCAGCGCA GAGTACAAGT ATACTTACAT 
GAGAGCACAC AGGATGAACT AGCACGGAAA TGTGAACAAG TCCTAATTGA AAAACATCTA 
GAGATTTTCC ACACTGAATT TCAGAATTTA CTAGATGCTG ATAAAAATGA AGACCTGGGC 
CGTATGTACA ATCTTGTTTC CCGAATCCAG GATGGTCTGG GAGAGCTGAA AAAGCTTTTA 
GAAACTCATA TTCACAATCA GGGACTTGCT GCCATAGAAA AATGTGGAGA AGCTGCACAA 
AATGATCCTA AAATGTATGT ACAGACTGTT TTGGATGTTC ACAAGAAATA CAATGCCTTA 
GTCATGTCAG CATTCAACAA TGATGCTGGA TTTGTGGCTG CACTAGATAA GGCTTGTGGT 
CGGTTTATAA ATAACAATGC TGTGACAAAA ATGGCTCAGT CTTCTAGCAA GTCACCAGAG 
TTGCTTGCCC GGTATTGTGA CTCTCTCTTG AAGAAGAGTT CCAAGAATCC AGAAGAAGCA 
GAGTTGGAGG ATACCCTTAA CCAAGTGATG GTGGTCTTTA AATACATAGA AGACAAGGAT 
GTGTTTCAGA AGTTCTATGC AAAAATGCTT GCAAAGCGAT TGGTTCATCA AAACAGTGCC 
AGTGATGATG CTGAAGCAAG TATGATATCA AAACTTAAGC AAGCCTGTGG GTTTGAGTAT 
ACATCAAAGC TTCAGAGAAT GTTTCAAGAT ATAGGTGTGA GCAAAGACCT TAATGAGCAG 
TTCAAAAAGC ACTTGACTAA TTCTGAGCCT TTAGACTTGG ACTTCAGCAT ACAGGTTCTC 
AGCTCTGGGT CATGGCCCTT CCAACAGTTC TGTACATTTG CTTTACCTTC AGAGTTGGAA 
CGCAGCTATC AAAGGTTTAC AGCTTTTTAT GCCAGCCGCC ACAGTGGAAG GAAGTTGACT 
TGGTTATACC AGTTGTCTAA AGGAGAACTG GTCACAAACT GCTTTAAAAA TAGATACACG 
TTGCAGGCAT CCACATTCCA GATGGCAATC CTGCTGCAGT ATAACACAGA AGATGCCTAC 
ACAGTGCAGC AACTGACAGA TAGCACACAG ATAAAAATGG ATATACTTGT GCAAGTTCTT 
CAAATTTTGC TGAAGTCTAA GTTATTGGTT TTAGAAGATG AAAATGCAAA TGTGGATGAT 
GTAGACTTGA AGCCAGATAT TTTAATAAAG CTCTACCTTG GTTATAAGAA TAAGAAGTTA 
AGAGTGAACA TAAATGTGCC CATGAAAACA GAACAGAAGC AGGAACAAGA AACAACACAC 
AAAAATATAG AGGAAGACCG AAAACTACTC ATTCAGGCAG CCATTGTGAG AATAATGAAG 
ATGAGGAAGG TTCTGAAACA CCAGCAGCTT CTTGGAGAAG TGTTGACTCA GCTGTCCTCC 
AGATTCAAAC CACGGGTCCC TGTTATCAAG AAATGCATTG ATATCCTAAT AGAAAAAGAG 
TATTTGGAGC GCGTGGATGG TGAAAAGGAC ACATACAGTT ACCTGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cul1 ( XM_012964856.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820310.1 Xenopus tropicalis cullin 1 (cul1), transcript variant X2, mRNA.

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CloneID	OXa25199
Clone ID Related Accession (Same CDS sequence)	XM_012964854.3 , XM_012964856.3 , NM_001045790.1
Accession Version	XM_012964856.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	cullin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964856.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCAA ACAGAAGTCA GAATCCACAT GGACTGAAAC AGATTGGCTT GGACCAAATA 
TGGGATGACC TGCGGGCCGG GATCCAACAA GTTTATACAA GACAGAGCAT GGCAAAGTCC 
AGATATATGG AACTCTATAC ACATGTTTAT AACTACTGCA CCAGTGTACA CCAATCTAAC 
CAAGCACGGG GGGCTGGAGT GCCGCCATCA AAATCTAAGA AAGGACAGAC TCCTGGAGGT 
GCCCAGTTTG TTGGTTTGGA GCTCTACAAA AGACTAAAGG AGTTCTTAAA GAATTACTTG 
ACAAATCTCC TAAAGGATGG AGAAGATTTG ATGGATGAGA GCGTACTGAA GTTTTACACA 
CAGCAATGGG AAGATTACAG GTTTTCAAGT AAAGTGCTGA ATGGAATCTG CGCATACCTC 
AACCGGCACT GGGTTCGCCG TGAGTGTGAT GAAGGCCGCA AAGGAATATA TGAAATATAT 
TCTCTGGCCT TAGTGACATG GAGGGATTGT CTGTTTAAGC CTTTGAATAA GCAGGTAACA 
AATGCTGTGC TGAAACTGAT TGAAAAGGAA CGAAATGGTG AAACAATAAA CACCAGGCTT 
ATAAGTGGAG TGGTGCAATC TTATGTTGAG CTTGGCTTGA ATGAAGATGA TGCATTTGCC 
AAAGGCCCAA CACTGACTGT TTATAAAGAG TCCTTTGAGT CTCAGTTTTT GGCAGACACA 
GAACGGTTTT ATACAAGAGA GAGCACAGAG TTTTTACAGC AGAACCCTGT CACAGAATAT 
ATGAAAAAGG CAGAAGCTCG GCTTTTGGAG GAGCAGCGCA GAGTACAAGT ATACTTACAT 
GAGAGCACAC AGGATGAACT AGCACGGAAA TGTGAACAAG TCCTAATTGA AAAACATCTA 
GAGATTTTCC ACACTGAATT TCAGAATTTA CTAGATGCTG ATAAAAATGA AGACCTGGGC 
CGTATGTACA ATCTTGTTTC CCGAATCCAG GATGGTCTGG GAGAGCTGAA AAAGCTTTTA 
GAAACTCATA TTCACAATCA GGGACTTGCT GCCATAGAAA AATGTGGAGA AGCTGCACAA 
AATGATCCTA AAATGTATGT ACAGACTGTT TTGGATGTTC ACAAGAAATA CAATGCCTTA 
GTCATGTCAG CATTCAACAA TGATGCTGGA TTTGTGGCTG CACTAGATAA GGCTTGTGGT 
CGGTTTATAA ATAACAATGC TGTGACAAAA ATGGCTCAGT CTTCTAGCAA GTCACCAGAG 
TTGCTTGCCC GGTATTGTGA CTCTCTCTTG AAGAAGAGTT CCAAGAATCC AGAAGAAGCA 
GAGTTGGAGG ATACCCTTAA CCAAGTGATG GTGGTCTTTA AATACATAGA AGACAAGGAT 
GTGTTTCAGA AGTTCTATGC AAAAATGCTT GCAAAGCGAT TGGTTCATCA AAACAGTGCC 
AGTGATGATG CTGAAGCAAG TATGATATCA AAACTTAAGC AAGCCTGTGG GTTTGAGTAT 
ACATCAAAGC TTCAGAGAAT GTTTCAAGAT ATAGGTGTGA GCAAAGACCT TAATGAGCAG 
TTCAAAAAGC ACTTGACTAA TTCTGAGCCT TTAGACTTGG ACTTCAGCAT ACAGGTTCTC 
AGCTCTGGGT CATGGCCCTT CCAACAGTTC TGTACATTTG CTTTACCTTC AGAGTTGGAA 
CGCAGCTATC AAAGGTTTAC AGCTTTTTAT GCCAGCCGCC ACAGTGGAAG GAAGTTGACT 
TGGTTATACC AGTTGTCTAA AGGAGAACTG GTCACAAACT GCTTTAAAAA TAGATACACG 
TTGCAGGCAT CCACATTCCA GATGGCAATC CTGCTGCAGT ATAACACAGA AGATGCCTAC 
ACAGTGCAGC AACTGACAGA TAGCACACAG ATAAAAATGG ATATACTTGT GCAAGTTCTT 
CAAATTTTGC TGAAGTCTAA GTTATTGGTT TTAGAAGATG AAAATGCAAA TGTGGATGAT 
GTAGACTTGA AGCCAGATAT TTTAATAAAG CTCTACCTTG GTTATAAGAA TAAGAAGTTA 
AGAGTGAACA TAAATGTGCC CATGAAAACA GAACAGAAGC AGGAACAAGA AACAACACAC 
AAAAATATAG AGGAAGACCG AAAACTACTC ATTCAGGCAG CCATTGTGAG AATAATGAAG 
ATGAGGAAGG TTCTGAAACA CCAGCAGCTT CTTGGAGAAG TGTTGACTCA GCTGTCCTCC 
AGATTCAAAC CACGGGTCCC TGTTATCAAG AAATGCATTG ATATCCTAAT AGAAAAAGAG 
TATTTGGAGC GCGTGGATGG TGAAAAGGAC ACATACAGTT ACCTGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820310.1
CDS	280..2610
Translation	MSSNRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFKPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAAQNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQFCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILVQVLQILLKSKLLVLEDENANVDDVDLKPDILIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

Target ORF information:

RefSeq Version	XM_012964856.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis cullin 1 (cul1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964856.3

ORF Insert Sequence:

ATGTCTTCAA ACAGAAGTCA GAATCCACAT GGACTGAAAC AGATTGGCTT GGACCAAATA 
TGGGATGACC TGCGGGCCGG GATCCAACAA GTTTATACAA GACAGAGCAT GGCAAAGTCC 
AGATATATGG AACTCTATAC ACATGTTTAT AACTACTGCA CCAGTGTACA CCAATCTAAC 
CAAGCACGGG GGGCTGGAGT GCCGCCATCA AAATCTAAGA AAGGACAGAC TCCTGGAGGT 
GCCCAGTTTG TTGGTTTGGA GCTCTACAAA AGACTAAAGG AGTTCTTAAA GAATTACTTG 
ACAAATCTCC TAAAGGATGG AGAAGATTTG ATGGATGAGA GCGTACTGAA GTTTTACACA 
CAGCAATGGG AAGATTACAG GTTTTCAAGT AAAGTGCTGA ATGGAATCTG CGCATACCTC 
AACCGGCACT GGGTTCGCCG TGAGTGTGAT GAAGGCCGCA AAGGAATATA TGAAATATAT 
TCTCTGGCCT TAGTGACATG GAGGGATTGT CTGTTTAAGC CTTTGAATAA GCAGGTAACA 
AATGCTGTGC TGAAACTGAT TGAAAAGGAA CGAAATGGTG AAACAATAAA CACCAGGCTT 
ATAAGTGGAG TGGTGCAATC TTATGTTGAG CTTGGCTTGA ATGAAGATGA TGCATTTGCC 
AAAGGCCCAA CACTGACTGT TTATAAAGAG TCCTTTGAGT CTCAGTTTTT GGCAGACACA 
GAACGGTTTT ATACAAGAGA GAGCACAGAG TTTTTACAGC AGAACCCTGT CACAGAATAT 
ATGAAAAAGG CAGAAGCTCG GCTTTTGGAG GAGCAGCGCA GAGTACAAGT ATACTTACAT 
GAGAGCACAC AGGATGAACT AGCACGGAAA TGTGAACAAG TCCTAATTGA AAAACATCTA 
GAGATTTTCC ACACTGAATT TCAGAATTTA CTAGATGCTG ATAAAAATGA AGACCTGGGC 
CGTATGTACA ATCTTGTTTC CCGAATCCAG GATGGTCTGG GAGAGCTGAA AAAGCTTTTA 
GAAACTCATA TTCACAATCA GGGACTTGCT GCCATAGAAA AATGTGGAGA AGCTGCACAA 
AATGATCCTA AAATGTATGT ACAGACTGTT TTGGATGTTC ACAAGAAATA CAATGCCTTA 
GTCATGTCAG CATTCAACAA TGATGCTGGA TTTGTGGCTG CACTAGATAA GGCTTGTGGT 
CGGTTTATAA ATAACAATGC TGTGACAAAA ATGGCTCAGT CTTCTAGCAA GTCACCAGAG 
TTGCTTGCCC GGTATTGTGA CTCTCTCTTG AAGAAGAGTT CCAAGAATCC AGAAGAAGCA 
GAGTTGGAGG ATACCCTTAA CCAAGTGATG GTGGTCTTTA AATACATAGA AGACAAGGAT 
GTGTTTCAGA AGTTCTATGC AAAAATGCTT GCAAAGCGAT TGGTTCATCA AAACAGTGCC 
AGTGATGATG CTGAAGCAAG TATGATATCA AAACTTAAGC AAGCCTGTGG GTTTGAGTAT 
ACATCAAAGC TTCAGAGAAT GTTTCAAGAT ATAGGTGTGA GCAAAGACCT TAATGAGCAG 
TTCAAAAAGC ACTTGACTAA TTCTGAGCCT TTAGACTTGG ACTTCAGCAT ACAGGTTCTC 
AGCTCTGGGT CATGGCCCTT CCAACAGTTC TGTACATTTG CTTTACCTTC AGAGTTGGAA 
CGCAGCTATC AAAGGTTTAC AGCTTTTTAT GCCAGCCGCC ACAGTGGAAG GAAGTTGACT 
TGGTTATACC AGTTGTCTAA AGGAGAACTG GTCACAAACT GCTTTAAAAA TAGATACACG 
TTGCAGGCAT CCACATTCCA GATGGCAATC CTGCTGCAGT ATAACACAGA AGATGCCTAC 
ACAGTGCAGC AACTGACAGA TAGCACACAG ATAAAAATGG ATATACTTGT GCAAGTTCTT 
CAAATTTTGC TGAAGTCTAA GTTATTGGTT TTAGAAGATG AAAATGCAAA TGTGGATGAT 
GTAGACTTGA AGCCAGATAT TTTAATAAAG CTCTACCTTG GTTATAAGAA TAAGAAGTTA 
AGAGTGAACA TAAATGTGCC CATGAAAACA GAACAGAAGC AGGAACAAGA AACAACACAC 
AAAAATATAG AGGAAGACCG AAAACTACTC ATTCAGGCAG CCATTGTGAG AATAATGAAG 
ATGAGGAAGG TTCTGAAACA CCAGCAGCTT CTTGGAGAAG TGTTGACTCA GCTGTCCTCC 
AGATTCAAAC CACGGGTCCC TGTTATCAAG AAATGCATTG ATATCCTAAT AGAAAAAGAG 
TATTTGGAGC GCGTGGATGG TGAAAAGGAC ACATACAGTT ACCTGGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A dominant-negative form of the E3 ubiquitin ligase Cullin-1 disrupts the correct allocation of cell fate in the neural crest lineage.
Development (Cambridge, England)133(3)559-68(2006 Feb)
Voigt J,Papalopulu N

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cul1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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