chrd.1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	chrd.1
Entrez Gene ID	549011
Full Name	chordin, gene 1
Synonyms	X-chordin,chd,chrd
General protein information	Preferred Names chordin, gene 1 Names chordin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012962976.2	XP_012818430.1	chordin isoform X1
NM_001142657.1	NP_001136129.1	chordin precursor
XM_012962977.3	XP_012818431.1	chordin isoform X2
XM_012962977.2	XP_012818431.1	chordin isoform X2
XM_012962976.3	XP_012818430.1	chordin isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHRD	XP_005639840.1
Danio rerio (zebrafish)	chd	NP_571048.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000472	XP_310620.4
Bos taurus (cattle)	CHRD	XP_002684938.2
Drosophila melanogaster (fruit fly)	sog	NP_001259578.1
Homo sapiens (human)	CHRD	NP_003732.2
Mus musculus (house mouse)	Chrd	NP_001264970.1
Gallus gallus (chicken)	CHRD	NP_990311.1
Pan troglodytes (chimpanzee)	CHRD	XP_516917.3
	CHRD	XP_001097729.2
Rattus norvegicus (Norway rat)	Chrd	NP_476475.1
Xenopus tropicalis (tropical clawed frog)	chrd	NP_001136129.1

Gene Ontology
Bibliography

Related articles in PubMed

Twisted gastrulation is required for forebrain specification and cooperates with Chordin to inhibit BMP signaling during X. tropicalis gastrulation.
Wills A, Harland RM, Khokha MK
Developmental biology289(1)166-78(2006 Jan)

The novel Smad-interacting protein Smicl regulates Chordin expression in the Xenopus embryo.
Collart C, Verschueren K, Rana A, Smith JC, Huylebroeck D
Development (Cambridge, England)132(20)4575-86(2005 Oct)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tsg acts cooperatively with chordin as a BMP antagonist in the context of the frog gastrula, and it acts to establish dorsal structures and particularly forebrain tissue during development.
Title: Twisted gastrulation is required for forebrain specification and cooperates with Chordin to inhibit BMP signaling during X. tropicalis gastrulation.

complex containing Smicl and the newly induced Xlim1 induces expression of Chordin
Title: The novel Smad-interacting protein Smicl regulates Chordin expression in the Xenopus embryo.

The following chrd.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chrd.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa22831	NM_001142657.1 Latest version!	Xenopus tropicalis chordin, gene 1 (chrd.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa27624	XM_012962976.2 Latest version!	Xenopus tropicalis chordin (chrd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa27625	XM_012962977.2 Latest version!	Xenopus tropicalis chordin (chrd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa58014	XM_012962976.3 Latest version!	Xenopus tropicalis chordin, gene 1 (chrd.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa58015	XM_012962977.3 Latest version!	Xenopus tropicalis chordin, gene 1 (chrd.1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chrd.1 ( NM_001142657.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001136129.1 Xenopus tropicalis chordin, gene 1 (chrd.1), mRNA.

Price & Availability↑

CloneID	OXa22831
Clone ID Related Accession (Same CDS sequence)	NM_001142657.1
Accession Version	NM_001142657.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chordin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168603.1. ##Evidence-Data-START## Transcript exon combination :: BC168603.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGCTTTGTT CCCCCATCTT ACTTCTGTGG ACCCTGTGGA TTATGGCTGT GGATTGCTCT 
CGCCCAAAGG TTTTCCTGCC CATTCAGCCT GAACAGGAGC CCCTCCAATC TAAGACACCA 
GCAGGTTGTA CGTTTGGTGG AAAATTTTAC TCTCTGGAGG ATTCATGGCA TCCAGATCTT 
GGGGATCCAT TCGGAGTTAT GCACTGTGTA TTATGCTATT GTGAGCCGCA GCGAAGTCGC 
CGGGGAAAAC CATCTGGCAA AGTCAGCTGC AAAAACATCA AACATGATTG CCCCTCACCC 
TCCTGTTCTA ACCCTGTCCT GCTTTCTGGA CATTGCTGCA AGACTTGTCC CAAAGCTCCT 
CCTCCTCCTA TAAAGAAATC AGACTTTGTT TTTGATGGAT TCGAGTATTT TCATGGGAAG 
GATGATGATC TTTATAATGA TCGCTCTTAC CTTAGTTCTG ATGATGTCAC TGTGGAGGAG 
AATCGTTCAG AATATGTTGC ATTATTAACA GCACCCAGCC ACGTGTGGCC CCCAGTTACC 
AGTGGAGTAG CCAAGGCTCG ATTCAATCTG CAGCGCTCCA GTTTGCTCTT CTCAATCACC 
TATAAATGGA TAGACAGACT TTCCCGAATC CGCTTCTCCG ATCTTGAAGG TTCTGTGCTG 
TTTGAACACC CAGTGCACAG AATGGGATCC CCACGAGGTG ACACTATCTG TGGTATTTGG 
AGGTCTCTAA ATCGCTCAAC TCTACGTCTT CTCCGCATGG GTCACATTCT GGTATCTTTG 
GTGACCACAA CACTTTCAGA GCCTGAAATA TCAGGCAAGA TTGTCAAACA CAAAGCCCTG 
TTTTCAGAGT CCTTCAGTGC ACTTCTTACC CCAGAAGACT CTGATGTAAC AGGAGGTGGA 
GGACTCGCAA TGCTTACACT AAGTGACGTG GATGACAATC TGCACTTTAT CCTTATGCTC 
AGAGGTCTTC GTGGTGGAGA AAGAGATCAG ATTCAAATAC TTGTGCAAAT CTTGCATCAG 
AACCATGTGA TACGGGAGCT TTACGCTAAC ATCTCTGCAC AGGAACAAGA CTTTGCAGAG 
GTGTTGCCAG ACCTCTCAAG CCGTGAGATG CAGTGGCTGG CCCAAGGTCA GCTGGAGATT 
TCAGTGCAGA CAGAAGGGAG ACGTGGGCAA CGCATGTCAG GCATCATCAC AGTCAGAAAA 
TCATGTGACA CACTGCAGAG TGTGTTGTCT GGTGGTGACA CTTTAAATCC CACCAAAACT 
GGAGCTGTGG GATCTGCAAG CATCACACTG CATGAAAATG GAACCATGGA ATATCAGATT 
CAAATTGCTG GTACCACAAG TGCTGTGACA GCAGTGACAC TGGAGACAAA ACCTCGCAGA 
AAAACAAAGA GAAATATACT GCATGATATG ACCAAGGACT ACCAAGATGG AAGGGTCTGG 
GGATACTGGA TAGATGCTAA TGCACGAGAC CTACATATGC TGTTGCAAAG TGAGCTCTTC 
CTTAATGTAG CAACAAAGGA TTTCCAGGAA GGGGAACTTA GAGGTCAAAT AACCCCTCTG 
CTATACAGCG GCCTTTGGGC CAGATATGAG CAGCTCCCAG TTCCTCTAGC TGGTCAGTTT 
GTGTCACCAC CCATCAGGAC AGGCTCAGCA GGTCACGCAT GGGTTTCACT GGATGAGCAC 
TGCCATCTGC ATTATCAGAT TGTGGTGACT GGTCTGGGTA AGGCAGAGGA TGCTGCACTG 
AATGCTCATC TCCATGGTTT TGCTGAGCTT GGTGAAGTCG GTGAGAGTAC CCCTGGGCAC 
AAGAGGTTGT TAAAGGGCTT CTATGGGTCA GAGGCCCAGG GTAGTGTAAA AGACCTTGAT 
CTTGAACTTT TGGGACATCT GAGCCGGGGC ACAGCATTTA TCCAAGTGAG CACCAAACTG 
AACCCTCGTG GTGAAATTCG AGGACAAGTG CACATACCTA ACAACTGTGA ATCTGGAGGA 
GTCTCTCTAA CTCCCGAGGA GCCTGAGTAT GAATATGAAT TATATGAGGA GGGAAGGCAG 
CGAGACCCAG AAGATCTTCG GAAAGATCCT AGAGCATGCT TTTTTGAAGG TCAACTAAGA 
GCCCATGGTT CACGATGGGC TCCAGATTAT GACAGGAAAT GCTCTGTGTG CAGCTGTCAG 
AAACGTACCG TGATCTGTGA TCCTATTGTG TGCCCACCTC TGAACTGCTC CCAGCCTGTC 
CATTTGTCAG ATCAGTGTTG TCCTGTGTGC GAAGAAAAAA AAGAAATTAG AGAGGTAAAA 
AAAACCGAGA GAGCTCGCAC AGCAAATGAA GGCTGCTTCT TTGATGGAGA TCGCTCATGG 
AAGGCGGCTG GTACACGTTG GCATCCTTTT GTTCCTCCAT TTGGTCTAAT TAAATGTGCT 
ATTTGCACCT GCAAGGGTTC CACTGGAGAA GTGCATTGTG AGAAGGTGAC CTGTCCACGA 
CTTTCCTGTA CCAACCCAAT TCGTGCTAAT CCATCTGATT GCTGCAAACA GTGCCCAGAG 
GAGCGAAGTC CTTTGGAGCT GGCAGACAGT ATGCAGTCAG ATGGACCGCG ATCATGCAAA 
TTTGGGCGTC ACTGGTACCC TAATAATGAG CGCTGGCATC CAACTGTGAC ACCCTTTGGA 
GAGATGAAGT GTGTTACATG TGCTTGTGTG GAGGGAGTTA CACCGTGTCG GAGACAGGAG 
TGTACAGGAA ATACATGTGG TACTGGTTCA AAGCGGGACA GATGTTGCAC CAAGTGCAAA 
GATGCCAAGC AAGATGAAGA TGAAAAGGTG AAATCAGAGG AGACAAGGAC TCCGTGGAGT 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136129.1
CDS	286..3111
Translation	MLCSPILLLWTLWIMAVDCSRPKVFLPIQPEQEPLQSKTPAGCTFGGKFYSLEDSWHPDLGDPFGVMHCVLCYCEPQRSRRGKPSGKVSCKNIKHDCPSPSCSNPVLLSGHCCKTCPKAPPPPIKKSDFVFDGFEYFHGKDDDLYNDRSYLSSDDVTVEENRSEYVALLTAPSHVWPPVTSGVAKARFNLQRSSLLFSITYKWIDRLSRIRFSDLEGSVLFEHPVHRMGSPRGDTICGIWRSLNRSTLRLLRMGHILVSLVTTTLSEPEISGKIVKHKALFSESFSALLTPEDSDVTGGGGLAMLTLSDVDDNLHFILMLRGLRGGERDQIQILVQILHQNHVIRELYANISAQEQDFAEVLPDLSSREMQWLAQGQLEISVQTEGRRGQRMSGIITVRKSCDTLQSVLSGGDTLNPTKTGAVGSASITLHENGTMEYQIQIAGTTSAVTAVTLETKPRRKTKRNILHDMTKDYQDGRVWGYWIDANARDLHMLLQSELFLNVATKDFQEGELRGQITPLLYSGLWARYEQLPVPLAGQFVSPPIRTGSAGHAWVSLDEHCHLHYQIVVTGLGKAEDAALNAHLHGFAELGEVGESTPGHKRLLKGFYGSEAQGSVKDLDLELLGHLSRGTAFIQVSTKLNPRGEIRGQVHIPNNCESGGVSLTPEEPEYEYELYEEGRQRDPEDLRKDPRACFFEGQLRAHGSRWAPDYDRKCSVCSCQKRTVICDPIVCPPLNCSQPVHLSDQCCPVCEEKKEIREVKKTERARTANEGCFFDGDRSWKAAGTRWHPFVPPFGLIKCAICTCKGSTGEVHCEKVTCPRLSCTNPIRANPSDCCKQCPEERSPLELADSMQSDGPRSCKFGRHWYPNNERWHPTVTPFGEMKCVTCACVEGVTPCRRQECTGNTCGTGSKRDRCCTKCKDAKQDEDEKVKSEETRTPWSF

Target ORF information:

RefSeq Version	NM_001142657.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chordin, gene 1 (chrd.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142657.1

ORF Insert Sequence:

ATGCTTTGTT CCCCCATCTT ACTTCTGTGG ACCCTGTGGA TTATGGCTGT GGATTGCTCT 
CGCCCAAAGG TTTTCCTGCC CATTCAGCCT GAACAGGAGC CCCTCCAATC TAAGACACCA 
GCAGGTTGTA CGTTTGGTGG AAAATTTTAC TCTCTGGAGG ATTCATGGCA TCCAGATCTT 
GGGGATCCAT TCGGAGTTAT GCACTGTGTA TTATGCTATT GTGAGCCGCA GCGAAGTCGC 
CGGGGAAAAC CATCTGGCAA AGTCAGCTGC AAAAACATCA AACATGATTG CCCCTCACCC 
TCCTGTTCTA ACCCTGTCCT GCTTTCTGGA CATTGCTGCA AGACTTGTCC CAAAGCTCCT 
CCTCCTCCTA TAAAGAAATC AGACTTTGTT TTTGATGGAT TCGAGTATTT TCATGGGAAG 
GATGATGATC TTTATAATGA TCGCTCTTAC CTTAGTTCTG ATGATGTCAC TGTGGAGGAG 
AATCGTTCAG AATATGTTGC ATTATTAACA GCACCCAGCC ACGTGTGGCC CCCAGTTACC 
AGTGGAGTAG CCAAGGCTCG ATTCAATCTG CAGCGCTCCA GTTTGCTCTT CTCAATCACC 
TATAAATGGA TAGACAGACT TTCCCGAATC CGCTTCTCCG ATCTTGAAGG TTCTGTGCTG 
TTTGAACACC CAGTGCACAG AATGGGATCC CCACGAGGTG ACACTATCTG TGGTATTTGG 
AGGTCTCTAA ATCGCTCAAC TCTACGTCTT CTCCGCATGG GTCACATTCT GGTATCTTTG 
GTGACCACAA CACTTTCAGA GCCTGAAATA TCAGGCAAGA TTGTCAAACA CAAAGCCCTG 
TTTTCAGAGT CCTTCAGTGC ACTTCTTACC CCAGAAGACT CTGATGTAAC AGGAGGTGGA 
GGACTCGCAA TGCTTACACT AAGTGACGTG GATGACAATC TGCACTTTAT CCTTATGCTC 
AGAGGTCTTC GTGGTGGAGA AAGAGATCAG ATTCAAATAC TTGTGCAAAT CTTGCATCAG 
AACCATGTGA TACGGGAGCT TTACGCTAAC ATCTCTGCAC AGGAACAAGA CTTTGCAGAG 
GTGTTGCCAG ACCTCTCAAG CCGTGAGATG CAGTGGCTGG CCCAAGGTCA GCTGGAGATT 
TCAGTGCAGA CAGAAGGGAG ACGTGGGCAA CGCATGTCAG GCATCATCAC AGTCAGAAAA 
TCATGTGACA CACTGCAGAG TGTGTTGTCT GGTGGTGACA CTTTAAATCC CACCAAAACT 
GGAGCTGTGG GATCTGCAAG CATCACACTG CATGAAAATG GAACCATGGA ATATCAGATT 
CAAATTGCTG GTACCACAAG TGCTGTGACA GCAGTGACAC TGGAGACAAA ACCTCGCAGA 
AAAACAAAGA GAAATATACT GCATGATATG ACCAAGGACT ACCAAGATGG AAGGGTCTGG 
GGATACTGGA TAGATGCTAA TGCACGAGAC CTACATATGC TGTTGCAAAG TGAGCTCTTC 
CTTAATGTAG CAACAAAGGA TTTCCAGGAA GGGGAACTTA GAGGTCAAAT AACCCCTCTG 
CTATACAGCG GCCTTTGGGC CAGATATGAG CAGCTCCCAG TTCCTCTAGC TGGTCAGTTT 
GTGTCACCAC CCATCAGGAC AGGCTCAGCA GGTCACGCAT GGGTTTCACT GGATGAGCAC 
TGCCATCTGC ATTATCAGAT TGTGGTGACT GGTCTGGGTA AGGCAGAGGA TGCTGCACTG 
AATGCTCATC TCCATGGTTT TGCTGAGCTT GGTGAAGTCG GTGAGAGTAC CCCTGGGCAC 
AAGAGGTTGT TAAAGGGCTT CTATGGGTCA GAGGCCCAGG GTAGTGTAAA AGACCTTGAT 
CTTGAACTTT TGGGACATCT GAGCCGGGGC ACAGCATTTA TCCAAGTGAG CACCAAACTG 
AACCCTCGTG GTGAAATTCG AGGACAAGTG CACATACCTA ACAACTGTGA ATCTGGAGGA 
GTCTCTCTAA CTCCCGAGGA GCCTGAGTAT GAATATGAAT TATATGAGGA GGGAAGGCAG 
CGAGACCCAG AAGATCTTCG GAAAGATCCT AGAGCATGCT TTTTTGAAGG TCAACTAAGA 
GCCCATGGTT CACGATGGGC TCCAGATTAT GACAGGAAAT GCTCTGTGTG CAGCTGTCAG 
AAACGTACCG TGATCTGTGA TCCTATTGTG TGCCCACCTC TGAACTGCTC CCAGCCTGTC 
CATTTGTCAG ATCAGTGTTG TCCTGTGTGC GAAGAAAAAA AAGAAATTAG AGAGGTAAAA 
AAAACCGAGA GAGCTCGCAC AGCAAATGAA GGCTGCTTCT TTGATGGAGA TCGCTCATGG 
AAGGCGGCTG GTACACGTTG GCATCCTTTT GTTCCTCCAT TTGGTCTAAT TAAATGTGCT 
ATTTGCACCT GCAAGGGTTC CACTGGAGAA GTGCATTGTG AGAAGGTGAC CTGTCCACGA 
CTTTCCTGTA CCAACCCAAT TCGTGCTAAT CCATCTGATT GCTGCAAACA GTGCCCAGAG 
GAGCGAAGTC CTTTGGAGCT GGCAGACAGT ATGCAGTCAG ATGGACCGCG ATCATGCAAA 
TTTGGGCGTC ACTGGTACCC TAATAATGAG CGCTGGCATC CAACTGTGAC ACCCTTTGGA 
GAGATGAAGT GTGTTACATG TGCTTGTGTG GAGGGAGTTA CACCGTGTCG GAGACAGGAG 
TGTACAGGAA ATACATGTGG TACTGGTTCA AAGCGGGACA GATGTTGCAC CAAGTGCAAA 
GATGCCAAGC AAGATGAAGA TGAAAAGGTG AAATCAGAGG AGACAAGGAC TCCGTGGAGT 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chrd.1 ( XM_012962976.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818430.1 Xenopus tropicalis chordin (chrd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa27624
Clone ID Related Accession (Same CDS sequence)	XM_012962976.2
Accession Version	XM_012962976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chordin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962976.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGTT CCCCCATCTT ACTTCTGTGG ACCCTGTGGA TTATGGCTGT GGATTGCTCT 
CGCCCAAAGG TTTTCCTGCC CATTCAGCCT GAACAGGAGC CCCTCCAATC TAAGACACCA 
GCAGGTTGTA CGTTTGGTGG AAAATTTTAC TCTCTGGAGG ATTCATGGCA TCCAGATCTT 
GGGGATCCAT TCGGAGTTAT GCACTGTGTA TTATGCTATT GTGAGCCGCA GCGAAGTCGC 
CGGGGAAAAC CATCTGGCAA AGTCAGCTGC AAAAACATCA AACATGATTG CCCCTCACCC 
TCCTGTTCTA ACCCTGTCCT GCTTTCTGGA CATTGCTGCA AGACTTGTCC CAAAGCTCCT 
CCTCCTCCTA TAAAGAAATC AGACTTTGTT TTTGATGGAT TCGAGTATTT TCATGGGAAG 
GATGATGATC TTTATAATGA TCGCTCTTAC CTTAGTTCTG ATGATGTCAC TGTGGAGGAG 
AATCGTTCAG AATATGTTGC ATTATTAACA GCACCCAGCC ACGTGTGGCC CCCAGTTACC 
AGTGGAGTAG CCAAGGCTCG ATTCAATCTG CAGCGCTCCA GTTTGCTCTT CTCAATCACC 
TATAAATGGA TAGACAGACT TTCCCGAATC CGCTTCTCCG ATCTTGAAGG TTCTGTGCTG 
TTTGAACACC CAGTGCACAG AATGGGATCC CCACGAGGTG ACACTATCTG TGGTATTTGG 
AGGTCTCTAA ATCGCTCAAC TCTACGTCTT CTCCGCATGG GTCACATTCT GGTATCTTTG 
GTGACCACAA CACTTTCAGA GCCTGAAATA TCAGGCAAGA TTGTCAAACA CAAAGCCCTG 
TTTTCAGAGT CCTTCAGTGC ACTTCTTACC CCAGAAGACT CTGATGTAAC AGGAGGTGGA 
GGACTCGCAA TGCTTACACT AAGTGACGTG GATGACAATC TGCACTTTAT CCTTATGCTC 
AGAGGTCTTC GTGGTGGAGA AAGAGATCAG ATTCAAATAC TTGTGCAAAT CTTGCATCAG 
AACCATGTGA TACGGGAGCT TTACGCTAAC ATCTCTGCAC AGGAACAAGA CTTTGCAGAG 
GTGTTGCCAG ACCTCTCAAG CCGTGAGATG CAGTGGCTGG CCCAAGGTCA GCTGGAGATT 
TCAGTGCAGA CAGAAGGGAG ACGTGGGCAA CGCATGTCAG GCATCATCAC AGTCAGAAAA 
TCATGTGACA CACTGCAGAG TGTGTTGTCT GGTGGTGACG CTTTAAATCC CACCAAAACT 
GGAGCTGTGG GATCTGCAAG CATCACACTG CATGAAAATG GAACCATGGA ATATCAGATT 
CAAATTGCTG GTACCACAAG TGCTGTGACA GCAGTGACAC TGGAGACAAA ACCTCGCAGA 
AAAACAAAGA GAAATATACT GCATGATATG ACCAAGGACT ACCAAGATGG AAGGGTCTGG 
GGATACTGGA TAGATGCTAA TGCACGAGAC CTACATATGC TGTTGCAAAG TGAGCTCTTC 
CTTAATGTAG CAACAAAGGA TTTCCAGGAA GGGGAACTTA GAGGTCAAAT AACCCCTCTG 
CTATACAGCG GCCTTTGGGC CAGATATGAG CAGCTCCCAG TTCCTCTAGC TGGTCAGTTT 
GTGTCACCAC CCATCAGGAC AGGCTCAGCA GGTCACGCAT GGGTTTCACT GGATGAGCAC 
TGCCATCTGC ATTATCAGAT TGTGGTGACT GGTCTGGGTA AGGCAGAGGA TGCTGCACTG 
AATGCTCATC TCCATGGTTT TGCTGAGCTT GGTGAAGTCG GTGAGAGTAC CCCTGGGCAC 
AAGAGGTTGT TAAAGGGCTT CTATGGGTCA GAGGCCCAGG GTAGTGTAAA AGACCTTGAT 
CTTGAACTTT TGGGACATCT GAGCCGGGGC ACAGCATTTA TTCAAGTGAG CACCAAACTG 
AACCCTCGTG GTGAAATTCG AGGACAAGTG CACATACCTA ACAACTGTGA ATCTGGAGGA 
GTCTCTCTAA CTCCCGAGGA GCCTGAGTAT GAATATGAAT TATATGAGGA GGGAAGGCAG 
CGAGACCCAG AAGATCTTCG GAAAGATCCT AGAGCATGCT TTTTTGAAGG TCAACTAAGA 
GCCCATGGTT CACGATGGGC TCCAGATTAT GACAGGAAAT GCTCTGTGTG CAGCTGTCAG 
AAACGTACCG TGATCTGTGA TCCTATTGTG TGCCCACCTC TGAACTGCTC CCAGCCTGTC 
CATTTGTCAG ATCAGTGTTG TCCTGTGTGC GAAGAAAAAA AAGAAATTAG AGAGGTAAAA 
AAAACCGAGA GAGCTCGCAC AGCAAATGAA GGCTGCTTCT TTGATGGAGA TCGCTCATGG 
AAGGCGGCTG GTACACGTTG GCATCCTTTT GTTCCTCCAT TTGGTCTAAT TAAATGTGCT 
ATTTGCACCT GCAAGGGTTC CACTGGAGAA GTGCATTGTG AGAAGGTGAC CTGTCCACGA 
CTTTCCTGTA CCAACCCAAT TCGTGCTAAT CCATCTGATT GCTGCAAACA GTGCCCAGTA 
GAGGAGCGAA GTCCTTTGGA GCTGGCAGAC AGTATGCAGT CAGATGGACC GCGATCATGC 
AAATTTGGGC GTCACTGGTA CCCTAATAAT GAGCGCTGGC ATCCAACTGT GACACCCTTT 
GGAGAGATGA AGTGTGTTAC ATGTGCTTGT GTGGAGGGAG TTACACAGTG TCGGAGACAG 
GAGTGTACAG GAAATACATG TGGTACTGGT TCAAAGCGGG ACAGATGTTG CACCAAGTGC 
AAAGATGCCA AGCAAGATGA AGATGAAAAG GTGAAATCAG AGGAGACAAG GACTCCGTGG 
AGTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818430.1
CDS	312..3140
Translation	MLCSPILLLWTLWIMAVDCSRPKVFLPIQPEQEPLQSKTPAGCTFGGKFYSLEDSWHPDLGDPFGVMHCVLCYCEPQRSRRGKPSGKVSCKNIKHDCPSPSCSNPVLLSGHCCKTCPKAPPPPIKKSDFVFDGFEYFHGKDDDLYNDRSYLSSDDVTVEENRSEYVALLTAPSHVWPPVTSGVAKARFNLQRSSLLFSITYKWIDRLSRIRFSDLEGSVLFEHPVHRMGSPRGDTICGIWRSLNRSTLRLLRMGHILVSLVTTTLSEPEISGKIVKHKALFSESFSALLTPEDSDVTGGGGLAMLTLSDVDDNLHFILMLRGLRGGERDQIQILVQILHQNHVIRELYANISAQEQDFAEVLPDLSSREMQWLAQGQLEISVQTEGRRGQRMSGIITVRKSCDTLQSVLSGGDALNPTKTGAVGSASITLHENGTMEYQIQIAGTTSAVTAVTLETKPRRKTKRNILHDMTKDYQDGRVWGYWIDANARDLHMLLQSELFLNVATKDFQEGELRGQITPLLYSGLWARYEQLPVPLAGQFVSPPIRTGSAGHAWVSLDEHCHLHYQIVVTGLGKAEDAALNAHLHGFAELGEVGESTPGHKRLLKGFYGSEAQGSVKDLDLELLGHLSRGTAFIQVSTKLNPRGEIRGQVHIPNNCESGGVSLTPEEPEYEYELYEEGRQRDPEDLRKDPRACFFEGQLRAHGSRWAPDYDRKCSVCSCQKRTVICDPIVCPPLNCSQPVHLSDQCCPVCEEKKEIREVKKTERARTANEGCFFDGDRSWKAAGTRWHPFVPPFGLIKCAICTCKGSTGEVHCEKVTCPRLSCTNPIRANPSDCCKQCPVEERSPLELADSMQSDGPRSCKFGRHWYPNNERWHPTVTPFGEMKCVTCACVEGVTQCRRQECTGNTCGTGSKRDRCCTKCKDAKQDEDEKVKSEETRTPWSF

Target ORF information:

RefSeq Version	XM_012962976.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chordin (chrd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962976.2

ORF Insert Sequence:

ATGCTTTGTT CCCCCATCTT ACTTCTGTGG ACCCTGTGGA TTATGGCTGT GGATTGCTCT 
CGCCCAAAGG TTTTCCTGCC CATTCAGCCT GAACAGGAGC CCCTCCAATC TAAGACACCA 
GCAGGTTGTA CGTTTGGTGG AAAATTTTAC TCTCTGGAGG ATTCATGGCA TCCAGATCTT 
GGGGATCCAT TCGGAGTTAT GCACTGTGTA TTATGCTATT GTGAGCCGCA GCGAAGTCGC 
CGGGGAAAAC CATCTGGCAA AGTCAGCTGC AAAAACATCA AACATGATTG CCCCTCACCC 
TCCTGTTCTA ACCCTGTCCT GCTTTCTGGA CATTGCTGCA AGACTTGTCC CAAAGCTCCT 
CCTCCTCCTA TAAAGAAATC AGACTTTGTT TTTGATGGAT TCGAGTATTT TCATGGGAAG 
GATGATGATC TTTATAATGA TCGCTCTTAC CTTAGTTCTG ATGATGTCAC TGTGGAGGAG 
AATCGTTCAG AATATGTTGC ATTATTAACA GCACCCAGCC ACGTGTGGCC CCCAGTTACC 
AGTGGAGTAG CCAAGGCTCG ATTCAATCTG CAGCGCTCCA GTTTGCTCTT CTCAATCACC 
TATAAATGGA TAGACAGACT TTCCCGAATC CGCTTCTCCG ATCTTGAAGG TTCTGTGCTG 
TTTGAACACC CAGTGCACAG AATGGGATCC CCACGAGGTG ACACTATCTG TGGTATTTGG 
AGGTCTCTAA ATCGCTCAAC TCTACGTCTT CTCCGCATGG GTCACATTCT GGTATCTTTG 
GTGACCACAA CACTTTCAGA GCCTGAAATA TCAGGCAAGA TTGTCAAACA CAAAGCCCTG 
TTTTCAGAGT CCTTCAGTGC ACTTCTTACC CCAGAAGACT CTGATGTAAC AGGAGGTGGA 
GGACTCGCAA TGCTTACACT AAGTGACGTG GATGACAATC TGCACTTTAT CCTTATGCTC 
AGAGGTCTTC GTGGTGGAGA AAGAGATCAG ATTCAAATAC TTGTGCAAAT CTTGCATCAG 
AACCATGTGA TACGGGAGCT TTACGCTAAC ATCTCTGCAC AGGAACAAGA CTTTGCAGAG 
GTGTTGCCAG ACCTCTCAAG CCGTGAGATG CAGTGGCTGG CCCAAGGTCA GCTGGAGATT 
TCAGTGCAGA CAGAAGGGAG ACGTGGGCAA CGCATGTCAG GCATCATCAC AGTCAGAAAA 
TCATGTGACA CACTGCAGAG TGTGTTGTCT GGTGGTGACG CTTTAAATCC CACCAAAACT 
GGAGCTGTGG GATCTGCAAG CATCACACTG CATGAAAATG GAACCATGGA ATATCAGATT 
CAAATTGCTG GTACCACAAG TGCTGTGACA GCAGTGACAC TGGAGACAAA ACCTCGCAGA 
AAAACAAAGA GAAATATACT GCATGATATG ACCAAGGACT ACCAAGATGG AAGGGTCTGG 
GGATACTGGA TAGATGCTAA TGCACGAGAC CTACATATGC TGTTGCAAAG TGAGCTCTTC 
CTTAATGTAG CAACAAAGGA TTTCCAGGAA GGGGAACTTA GAGGTCAAAT AACCCCTCTG 
CTATACAGCG GCCTTTGGGC CAGATATGAG CAGCTCCCAG TTCCTCTAGC TGGTCAGTTT 
GTGTCACCAC CCATCAGGAC AGGCTCAGCA GGTCACGCAT GGGTTTCACT GGATGAGCAC 
TGCCATCTGC ATTATCAGAT TGTGGTGACT GGTCTGGGTA AGGCAGAGGA TGCTGCACTG 
AATGCTCATC TCCATGGTTT TGCTGAGCTT GGTGAAGTCG GTGAGAGTAC CCCTGGGCAC 
AAGAGGTTGT TAAAGGGCTT CTATGGGTCA GAGGCCCAGG GTAGTGTAAA AGACCTTGAT 
CTTGAACTTT TGGGACATCT GAGCCGGGGC ACAGCATTTA TTCAAGTGAG CACCAAACTG 
AACCCTCGTG GTGAAATTCG AGGACAAGTG CACATACCTA ACAACTGTGA ATCTGGAGGA 
GTCTCTCTAA CTCCCGAGGA GCCTGAGTAT GAATATGAAT TATATGAGGA GGGAAGGCAG 
CGAGACCCAG AAGATCTTCG GAAAGATCCT AGAGCATGCT TTTTTGAAGG TCAACTAAGA 
GCCCATGGTT CACGATGGGC TCCAGATTAT GACAGGAAAT GCTCTGTGTG CAGCTGTCAG 
AAACGTACCG TGATCTGTGA TCCTATTGTG TGCCCACCTC TGAACTGCTC CCAGCCTGTC 
CATTTGTCAG ATCAGTGTTG TCCTGTGTGC GAAGAAAAAA AAGAAATTAG AGAGGTAAAA 
AAAACCGAGA GAGCTCGCAC AGCAAATGAA GGCTGCTTCT TTGATGGAGA TCGCTCATGG 
AAGGCGGCTG GTACACGTTG GCATCCTTTT GTTCCTCCAT TTGGTCTAAT TAAATGTGCT 
ATTTGCACCT GCAAGGGTTC CACTGGAGAA GTGCATTGTG AGAAGGTGAC CTGTCCACGA 
CTTTCCTGTA CCAACCCAAT TCGTGCTAAT CCATCTGATT GCTGCAAACA GTGCCCAGTA 
GAGGAGCGAA GTCCTTTGGA GCTGGCAGAC AGTATGCAGT CAGATGGACC GCGATCATGC 
AAATTTGGGC GTCACTGGTA CCCTAATAAT GAGCGCTGGC ATCCAACTGT GACACCCTTT 
GGAGAGATGA AGTGTGTTAC ATGTGCTTGT GTGGAGGGAG TTACACAGTG TCGGAGACAG 
GAGTGTACAG GAAATACATG TGGTACTGGT TCAAAGCGGG ACAGATGTTG CACCAAGTGC 
AAAGATGCCA AGCAAGATGA AGATGAAAAG GTGAAATCAG AGGAGACAAG GACTCCGTGG 
AGTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chrd.1 ( XM_012962977.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818431.1 Xenopus tropicalis chordin (chrd), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa27625
Clone ID Related Accession (Same CDS sequence)	XM_012962977.2
Accession Version	XM_012962977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chordin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGTT CCCCCATCTT ACTTCTGTGG ACCCTGTGGA TTATGGCTGT GGATTGCTCT 
CGCCCAAAGG TTTTCCTGCC CATTCAGCCT GAACAGGAGC CCCTCCAATC TAAGACACCA 
GCAGGTTGTA CGTTTGGTGG AAAATTTTAC TCTCTGGAGG ATTCATGGCA TCCAGATCTT 
GGGGATCCAT TCGGAGTTAT GCACTGTGTA TTATGCTATT GTGAGCCGCG AAGTCGCCGG 
GGAAAACCAT CTGGCAAAGT CAGCTGCAAA AACATCAAAC ATGATTGCCC CTCACCCTCC 
TGTTCTAACC CTGTCCTGCT TTCTGGACAT TGCTGCAAGA CTTGTCCCAA AGCTCCTCCT 
CCTCCTATAA AGAAATCAGA CTTTGTTTTT GATGGATTCG AGTATTTTCA TGGGAAGGAT 
GATGATCTTT ATAATGATCG CTCTTACCTT AGTTCTGATG ATGTCACTGT GGAGGAGAAT 
CGTTCAGAAT ATGTTGCATT ATTAACAGCA CCCAGCCACG TGTGGCCCCC AGTTACCAGT 
GGAGTAGCCA AGGCTCGATT CAATCTGCAG CGCTCCAGTT TGCTCTTCTC AATCACCTAT 
AAATGGATAG ACAGACTTTC CCGAATCCGC TTCTCCGATC TTGAAGGTTC TGTGCTGTTT 
GAACACCCAG TGCACAGAAT GGGATCCCCA CGAGGTGACA CTATCTGTGG TATTTGGAGG 
TCTCTAAATC GCTCAACTCT ACGTCTTCTC CGCATGGGTC ACATTCTGGT ATCTTTGGTG 
ACCACAACAC TTTCAGAGCC TGAAATATCA GGCAAGATTG TCAAACACAA AGCCCTGTTT 
TCAGAGTCCT TCAGTGCACT TCTTACCCCA GAAGACTCTG ATGTAACAGG AGGTGGAGGA 
CTCGCAATGC TTACACTAAG TGACGTGGAT GACAATCTGC ACTTTATCCT TATGCTCAGA 
GGTCTTCGTG GTGGAGAAAG AGATCAGATT CAAATACTTG TGCAAATCTT GCATCAGAAC 
CATGTGATAC GGGAGCTTTA CGCTAACATC TCTGCACAGG AACAAGACTT TGCAGAGGTG 
TTGCCAGACC TCTCAAGCCG TGAGATGCAG TGGCTGGCCC AAGGTCAGCT GGAGATTTCA 
GTGCAGACAG AAGGGAGACG TGGGCAACGC ATGTCAGGCA TCATCACAGT CAGAAAATCA 
TGTGACACAC TGCAGAGTGT GTTGTCTGGT GGTGACGCTT TAAATCCCAC CAAAACTGGA 
GCTGTGGGAT CTGCAAGCAT CACACTGCAT GAAAATGGAA CCATGGAATA TCAGATTCAA 
ATTGCTGGTA CCACAAGTGC TGTGACAGCA GTGACACTGG AGACAAAACC TCGCAGAAAA 
ACAAAGAGAA ATATACTGCA TGATATGACC AAGGACTACC AAGATGGAAG GGTCTGGGGA 
TACTGGATAG ATGCTAATGC ACGAGACCTA CATATGCTGT TGCAAAGTGA GCTCTTCCTT 
AATGTAGCAA CAAAGGATTT CCAGGAAGGG GAACTTAGAG GTCAAATAAC CCCTCTGCTA 
TACAGCGGCC TTTGGGCCAG ATATGAGCAG CTCCCAGTTC CTCTAGCTGG TCAGTTTGTG 
TCACCACCCA TCAGGACAGG CTCAGCAGGT CACGCATGGG TTTCACTGGA TGAGCACTGC 
CATCTGCATT ATCAGATTGT GGTGACTGGT CTGGGTAAGG CAGAGGATGC TGCACTGAAT 
GCTCATCTCC ATGGTTTTGC TGAGCTTGGT GAAGTCGGTG AGAGTACCCC TGGGCACAAG 
AGGTTGTTAA AGGGCTTCTA TGGGTCAGAG GCCCAGGGTA GTGTAAAAGA CCTTGATCTT 
GAACTTTTGG GACATCTGAG CCGGGGCACA GCATTTATTC AAGTGAGCAC CAAACTGAAC 
CCTCGTGGTG AAATTCGAGG ACAAGTGCAC ATACCTAACA ACTGTGAATC TGGAGGAGTC 
TCTCTAACTC CCGAGGAGCC TGAGTATGAA TATGAATTAT ATGAGGAGGG AAGGCAGCGA 
GACCCAGAAG ATCTTCGGAA AGATCCTAGA GCATGCTTTT TTGAAGGTCA ACTAAGAGCC 
CATGGTTCAC GATGGGCTCC AGATTATGAC AGGAAATGCT CTGTGTGCAG CTGTCAGAAA 
CGTACCGTGA TCTGTGATCC TATTGTGTGC CCACCTCTGA ACTGCTCCCA GCCTGTCCAT 
TTGTCAGATC AGTGTTGTCC TGTGTGCGAA GAAAAAAAAG AAATTAGAGA GGTAAAAAAA 
ACCGAGAGAG CTCGCACAGC AAATGAAGGC TGCTTCTTTG ATGGAGATCG CTCATGGAAG 
GCGGCTGGTA CACGTTGGCA TCCTTTTGTT CCTCCATTTG GTCTAATTAA ATGTGCTATT 
TGCACCTGCA AGGGTTCCAC TGGAGAAGTG CATTGTGAGA AGGTGACCTG TCCACGACTT 
TCCTGTACCA ACCCAATTCG TGCTAATCCA TCTGATTGCT GCAAACAGTG CCCAGTAGAG 
GAGCGAAGTC CTTTGGAGCT GGCAGACAGT ATGCAGTCAG ATGGACCGCG ATCATGCAAA 
TTTGGGCGTC ACTGGTACCC TAATAATGAG CGCTGGCATC CAACTGTGAC ACCCTTTGGA 
GAGATGAAGT GTGTTACATG TGCTTGTGTG GAGGGAGTTA CACAGTGTCG GAGACAGGAG 
TGTACAGGAA ATACATGTGG TACTGGTTCA AAGCGGGACA GATGTTGCAC CAAGTGCAAA 
GATGCCAAGC AAGATGAAGA TGAAAAGGTG AAATCAGAGG AGACAAGGAC TCCGTGGAGT 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818431.1
CDS	312..3137
Translation	MLCSPILLLWTLWIMAVDCSRPKVFLPIQPEQEPLQSKTPAGCTFGGKFYSLEDSWHPDLGDPFGVMHCVLCYCEPRSRRGKPSGKVSCKNIKHDCPSPSCSNPVLLSGHCCKTCPKAPPPPIKKSDFVFDGFEYFHGKDDDLYNDRSYLSSDDVTVEENRSEYVALLTAPSHVWPPVTSGVAKARFNLQRSSLLFSITYKWIDRLSRIRFSDLEGSVLFEHPVHRMGSPRGDTICGIWRSLNRSTLRLLRMGHILVSLVTTTLSEPEISGKIVKHKALFSESFSALLTPEDSDVTGGGGLAMLTLSDVDDNLHFILMLRGLRGGERDQIQILVQILHQNHVIRELYANISAQEQDFAEVLPDLSSREMQWLAQGQLEISVQTEGRRGQRMSGIITVRKSCDTLQSVLSGGDALNPTKTGAVGSASITLHENGTMEYQIQIAGTTSAVTAVTLETKPRRKTKRNILHDMTKDYQDGRVWGYWIDANARDLHMLLQSELFLNVATKDFQEGELRGQITPLLYSGLWARYEQLPVPLAGQFVSPPIRTGSAGHAWVSLDEHCHLHYQIVVTGLGKAEDAALNAHLHGFAELGEVGESTPGHKRLLKGFYGSEAQGSVKDLDLELLGHLSRGTAFIQVSTKLNPRGEIRGQVHIPNNCESGGVSLTPEEPEYEYELYEEGRQRDPEDLRKDPRACFFEGQLRAHGSRWAPDYDRKCSVCSCQKRTVICDPIVCPPLNCSQPVHLSDQCCPVCEEKKEIREVKKTERARTANEGCFFDGDRSWKAAGTRWHPFVPPFGLIKCAICTCKGSTGEVHCEKVTCPRLSCTNPIRANPSDCCKQCPVEERSPLELADSMQSDGPRSCKFGRHWYPNNERWHPTVTPFGEMKCVTCACVEGVTQCRRQECTGNTCGTGSKRDRCCTKCKDAKQDEDEKVKSEETRTPWSF

Target ORF information:

RefSeq Version	XM_012962977.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chordin (chrd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962977.2

ORF Insert Sequence:

ATGCTTTGTT CCCCCATCTT ACTTCTGTGG ACCCTGTGGA TTATGGCTGT GGATTGCTCT 
CGCCCAAAGG TTTTCCTGCC CATTCAGCCT GAACAGGAGC CCCTCCAATC TAAGACACCA 
GCAGGTTGTA CGTTTGGTGG AAAATTTTAC TCTCTGGAGG ATTCATGGCA TCCAGATCTT 
GGGGATCCAT TCGGAGTTAT GCACTGTGTA TTATGCTATT GTGAGCCGCG AAGTCGCCGG 
GGAAAACCAT CTGGCAAAGT CAGCTGCAAA AACATCAAAC ATGATTGCCC CTCACCCTCC 
TGTTCTAACC CTGTCCTGCT TTCTGGACAT TGCTGCAAGA CTTGTCCCAA AGCTCCTCCT 
CCTCCTATAA AGAAATCAGA CTTTGTTTTT GATGGATTCG AGTATTTTCA TGGGAAGGAT 
GATGATCTTT ATAATGATCG CTCTTACCTT AGTTCTGATG ATGTCACTGT GGAGGAGAAT 
CGTTCAGAAT ATGTTGCATT ATTAACAGCA CCCAGCCACG TGTGGCCCCC AGTTACCAGT 
GGAGTAGCCA AGGCTCGATT CAATCTGCAG CGCTCCAGTT TGCTCTTCTC AATCACCTAT 
AAATGGATAG ACAGACTTTC CCGAATCCGC TTCTCCGATC TTGAAGGTTC TGTGCTGTTT 
GAACACCCAG TGCACAGAAT GGGATCCCCA CGAGGTGACA CTATCTGTGG TATTTGGAGG 
TCTCTAAATC GCTCAACTCT ACGTCTTCTC CGCATGGGTC ACATTCTGGT ATCTTTGGTG 
ACCACAACAC TTTCAGAGCC TGAAATATCA GGCAAGATTG TCAAACACAA AGCCCTGTTT 
TCAGAGTCCT TCAGTGCACT TCTTACCCCA GAAGACTCTG ATGTAACAGG AGGTGGAGGA 
CTCGCAATGC TTACACTAAG TGACGTGGAT GACAATCTGC ACTTTATCCT TATGCTCAGA 
GGTCTTCGTG GTGGAGAAAG AGATCAGATT CAAATACTTG TGCAAATCTT GCATCAGAAC 
CATGTGATAC GGGAGCTTTA CGCTAACATC TCTGCACAGG AACAAGACTT TGCAGAGGTG 
TTGCCAGACC TCTCAAGCCG TGAGATGCAG TGGCTGGCCC AAGGTCAGCT GGAGATTTCA 
GTGCAGACAG AAGGGAGACG TGGGCAACGC ATGTCAGGCA TCATCACAGT CAGAAAATCA 
TGTGACACAC TGCAGAGTGT GTTGTCTGGT GGTGACGCTT TAAATCCCAC CAAAACTGGA 
GCTGTGGGAT CTGCAAGCAT CACACTGCAT GAAAATGGAA CCATGGAATA TCAGATTCAA 
ATTGCTGGTA CCACAAGTGC TGTGACAGCA GTGACACTGG AGACAAAACC TCGCAGAAAA 
ACAAAGAGAA ATATACTGCA TGATATGACC AAGGACTACC AAGATGGAAG GGTCTGGGGA 
TACTGGATAG ATGCTAATGC ACGAGACCTA CATATGCTGT TGCAAAGTGA GCTCTTCCTT 
AATGTAGCAA CAAAGGATTT CCAGGAAGGG GAACTTAGAG GTCAAATAAC CCCTCTGCTA 
TACAGCGGCC TTTGGGCCAG ATATGAGCAG CTCCCAGTTC CTCTAGCTGG TCAGTTTGTG 
TCACCACCCA TCAGGACAGG CTCAGCAGGT CACGCATGGG TTTCACTGGA TGAGCACTGC 
CATCTGCATT ATCAGATTGT GGTGACTGGT CTGGGTAAGG CAGAGGATGC TGCACTGAAT 
GCTCATCTCC ATGGTTTTGC TGAGCTTGGT GAAGTCGGTG AGAGTACCCC TGGGCACAAG 
AGGTTGTTAA AGGGCTTCTA TGGGTCAGAG GCCCAGGGTA GTGTAAAAGA CCTTGATCTT 
GAACTTTTGG GACATCTGAG CCGGGGCACA GCATTTATTC AAGTGAGCAC CAAACTGAAC 
CCTCGTGGTG AAATTCGAGG ACAAGTGCAC ATACCTAACA ACTGTGAATC TGGAGGAGTC 
TCTCTAACTC CCGAGGAGCC TGAGTATGAA TATGAATTAT ATGAGGAGGG AAGGCAGCGA 
GACCCAGAAG ATCTTCGGAA AGATCCTAGA GCATGCTTTT TTGAAGGTCA ACTAAGAGCC 
CATGGTTCAC GATGGGCTCC AGATTATGAC AGGAAATGCT CTGTGTGCAG CTGTCAGAAA 
CGTACCGTGA TCTGTGATCC TATTGTGTGC CCACCTCTGA ACTGCTCCCA GCCTGTCCAT 
TTGTCAGATC AGTGTTGTCC TGTGTGCGAA GAAAAAAAAG AAATTAGAGA GGTAAAAAAA 
ACCGAGAGAG CTCGCACAGC AAATGAAGGC TGCTTCTTTG ATGGAGATCG CTCATGGAAG 
GCGGCTGGTA CACGTTGGCA TCCTTTTGTT CCTCCATTTG GTCTAATTAA ATGTGCTATT 
TGCACCTGCA AGGGTTCCAC TGGAGAAGTG CATTGTGAGA AGGTGACCTG TCCACGACTT 
TCCTGTACCA ACCCAATTCG TGCTAATCCA TCTGATTGCT GCAAACAGTG CCCAGTAGAG 
GAGCGAAGTC CTTTGGAGCT GGCAGACAGT ATGCAGTCAG ATGGACCGCG ATCATGCAAA 
TTTGGGCGTC ACTGGTACCC TAATAATGAG CGCTGGCATC CAACTGTGAC ACCCTTTGGA 
GAGATGAAGT GTGTTACATG TGCTTGTGTG GAGGGAGTTA CACAGTGTCG GAGACAGGAG 
TGTACAGGAA ATACATGTGG TACTGGTTCA AAGCGGGACA GATGTTGCAC CAAGTGCAAA 
GATGCCAAGC AAGATGAAGA TGAAAAGGTG AAATCAGAGG AGACAAGGAC TCCGTGGAGT 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chrd.1 ( XM_012962976.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818430.1 Xenopus tropicalis chordin, gene 1 (chrd.1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa58014
Clone ID Related Accession (Same CDS sequence)	XM_012962976.3
Accession Version	XM_012962976.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chordin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962976.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGTT CCCCCATCTT ACTTCTGTGG ACCCTGTGGA TTATGGCTGT GGATTGCTCT 
CGCCCAAAGG TTTTCCTGCC CATTCAGCCT GAACAGGAGC CCCTCCAATC TAAGACACCA 
GCAGGTTGTA CGTTTGGTGG AAAATTTTAC TCTCTGGAGG ATTCATGGCA TCCAGATCTT 
GGGGATCCAT TCGGAGTTAT GCACTGTGTA TTATGCTATT GTGAGCCGCA GCGAAGTCGC 
CGGGGAAAAC CATCTGGCAA AGTCAGCTGC AAAAACATCA AACATGATTG CCCCTCACCC 
TCCTGTTCTA ACCCTGTCCT GCTTTCTGGA CATTGCTGCA AGACTTGTCC CAAAGCTCCT 
CCTCCTCCTA TAAAGAAATC AGACTTTGTT TTTGATGGAT TCGAGTATTT TCATGGGAAG 
GATGATGATC TTTATAATGA TCGCTCTTAC CTTAGTTCTG ATGATGTCAC TGTGGAGGAG 
AATCGTTCAG AATATGTTGC ATTATTAACA GCACCCAGCC ACGTGTGGCC CCCAGTTACC 
AGTGGAGTAG CCAAGGCTCG ATTCAATCTG CAGCGCTCCA GTTTGCTCTT CTCAATCACC 
TATAAATGGA TAGACAGACT TTCCCGAATC CGCTTCTCCG ATCTTGAAGG TTCTGTGCTG 
TTTGAACACC CAGTGCACAG AATGGGATCC CCACGAGGTG ACACTATCTG TGGTATTTGG 
AGGTCTCTAA ATCGCTCAAC TCTACGTCTT CTCCGCATGG GTCACATTCT GGTATCTTTG 
GTGACCACAA CACTTTCAGA GCCTGAAATA TCAGGCAAGA TTGTCAAACA CAAAGCCCTG 
TTTTCAGAGT CCTTCAGTGC ACTTCTTACC CCAGAAGACT CTGATGTAAC AGGAGGTGGA 
GGACTCGCAA TGCTTACACT AAGTGACGTG GATGACAATC TGCACTTTAT CCTTATGCTC 
AGAGGTCTTC GTGGTGGAGA AAGAGATCAG ATTCAAATAC TTGTGCAAAT CTTGCATCAG 
AACCATGTGA TACGGGAGCT TTACGCTAAC ATCTCTGCAC AGGAACAAGA CTTTGCAGAG 
GTGTTGCCAG ACCTTTCAAG CCGTGAGATG CAGTGGCTGG CCCAAGGTCA GCTGGAGATT 
TCAGTGCAGA CAGAAGGGAG ACGTGGGCAA CGCATGTCAG GCATCATCAC AGTCAGAAAA 
TCATGTGACA CACTGCAGAG TGTGTTGTCT GGTGGTGACG CTTTAAATCC CACCAAAACT 
GGAGCTGTGG GATCTGCAAG CATCACACTG CATGAAAATG GAACCATGGA ATATCAGATT 
CAAATTGCTG GTACCACAAG TGCTGTGACA GCAGTGACAC TGGAGACAAA ACCTCGCAGA 
AAAACAAAGA GAAATATACT GCATGATATG ACCAAGGACT ACCAAGATGG AAGGGTCTGG 
GGATACTGGA TAGATGCTAA TGCACGAGAC CTACATATGC TGTTGCAAAG TGAGCTCTTC 
CTTAATGTAG CAACAAAGGA TTTCCAGGAA GGGGAACTTA GAGGTCAAAT AACCCCTCTG 
CTATACAGCG GCCTTTGGGC CAGATATGAG CAGCTCCCAG TTCCTCTAGC TGGTCAGTTT 
GTGTCACCAC CCATCAGGAC AGGCTCAGCA GGTCACGCAT GGGTTTCACT GGATGAGCAC 
TGCCATCTGC ATTATCAGAT TGTGGTGACT GGTCTGGGTA AGGCAGAGGA TGCTGCACTG 
AATGCTCATC TCCATGGTTT TGCTGAGCTT GGTGAAGTCG GTGAGAGTAC CCCTGGGCAC 
AAGAGGTTGT TAAAGGGCTT CTATGGGTCA GAGGCCCAGG GTAGTGTAAA AGACCTTGAT 
CTTGAACTTT TGGGACATCT GAGCCGGGGC ACAGCATTTA TTCAAGTGAG CACCAAACTG 
AACCCTCGTG GTGAAATTCG AGGACAAGTG CACATACCTA ACAACTGTGA ATCTGGAGGA 
GTCTCTCTAA CTCCCGAGGA GCCTGAGTAT GAATATGAAT TATATGAGGA GGGAAGGCAG 
CGAGACCCAG AAGATCTTCG GAAAGATCCT AGAGCATGCT TTTTTGAAGG TCAACTAAGA 
GCCCATGGTT CACGATGGGC TCCAGATTAT GACAGGAAAT GCTCTGTGTG CAGCTGTCAG 
AAACGTACCG TGATCTGTGA TCCTATTGTG TGCCCACCTC TGAACTGCTC CCAGCCTGTC 
CATTTGTCAG ATCAGTGTTG TCCTGTGTGC GAAGAAAAAA AAGAAATTAG AGAGGTAAAA 
AAAACCGAGA GAGCTCGCAC AGCAAATGAA GGCTGCTTCT TTGATGGAGA TCGCTCATGG 
AAGGCGGCTG GTACACGTTG GCATCCTTTT GTTCCTCCAT TTGGTCTAAT TAAATGTGCT 
ATTTGCACCT GCAAGGGTTC CACTGGAGAA GTGCATTGTG AGAAGGTGAC CTGTCCACGA 
CTTTCCTGTA CCAACCCAAT TCGTGCTAAT CCATCTGATT GCTGCAAACA GTGCCCAGTA 
GAGGAGCGAA GTCCTTTGGA GCTGGCAGAC AGTATGCAGT CAGATGGACC GCGATCATGC 
AAATTTGGGC GTCACTGGTA CCCTAATAAT GAGCGCTGGC ATCCAACTGT GACACCCTTT 
GGAGAGATGA AGTGTGTTAC ATGTGCTTGT GTGGAGGGAG TTACACAGTG TCGGAGACAG 
GAGTGTACAG GAAATACATG TGGTACTGGT TCAAAGCGGG ACAGATGTTG CACCAAGTGC 
AAAGATGCCA AGCAAGATGA AGATGAAAAG GTGAAATCAG AGGAGACAAG GACTCCGTGG 
AGTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818430.1
CDS	468..3296
Translation	MLCSPILLLWTLWIMAVDCSRPKVFLPIQPEQEPLQSKTPAGCTFGGKFYSLEDSWHPDLGDPFGVMHCVLCYCEPQRSRRGKPSGKVSCKNIKHDCPSPSCSNPVLLSGHCCKTCPKAPPPPIKKSDFVFDGFEYFHGKDDDLYNDRSYLSSDDVTVEENRSEYVALLTAPSHVWPPVTSGVAKARFNLQRSSLLFSITYKWIDRLSRIRFSDLEGSVLFEHPVHRMGSPRGDTICGIWRSLNRSTLRLLRMGHILVSLVTTTLSEPEISGKIVKHKALFSESFSALLTPEDSDVTGGGGLAMLTLSDVDDNLHFILMLRGLRGGERDQIQILVQILHQNHVIRELYANISAQEQDFAEVLPDLSSREMQWLAQGQLEISVQTEGRRGQRMSGIITVRKSCDTLQSVLSGGDALNPTKTGAVGSASITLHENGTMEYQIQIAGTTSAVTAVTLETKPRRKTKRNILHDMTKDYQDGRVWGYWIDANARDLHMLLQSELFLNVATKDFQEGELRGQITPLLYSGLWARYEQLPVPLAGQFVSPPIRTGSAGHAWVSLDEHCHLHYQIVVTGLGKAEDAALNAHLHGFAELGEVGESTPGHKRLLKGFYGSEAQGSVKDLDLELLGHLSRGTAFIQVSTKLNPRGEIRGQVHIPNNCESGGVSLTPEEPEYEYELYEEGRQRDPEDLRKDPRACFFEGQLRAHGSRWAPDYDRKCSVCSCQKRTVICDPIVCPPLNCSQPVHLSDQCCPVCEEKKEIREVKKTERARTANEGCFFDGDRSWKAAGTRWHPFVPPFGLIKCAICTCKGSTGEVHCEKVTCPRLSCTNPIRANPSDCCKQCPVEERSPLELADSMQSDGPRSCKFGRHWYPNNERWHPTVTPFGEMKCVTCACVEGVTQCRRQECTGNTCGTGSKRDRCCTKCKDAKQDEDEKVKSEETRTPWSF

Target ORF information:

RefSeq Version	XM_012962976.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chordin, gene 1 (chrd.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962976.3

ORF Insert Sequence:

ATGCTTTGTT CCCCCATCTT ACTTCTGTGG ACCCTGTGGA TTATGGCTGT GGATTGCTCT 
CGCCCAAAGG TTTTCCTGCC CATTCAGCCT GAACAGGAGC CCCTCCAATC TAAGACACCA 
GCAGGTTGTA CGTTTGGTGG AAAATTTTAC TCTCTGGAGG ATTCATGGCA TCCAGATCTT 
GGGGATCCAT TCGGAGTTAT GCACTGTGTA TTATGCTATT GTGAGCCGCA GCGAAGTCGC 
CGGGGAAAAC CATCTGGCAA AGTCAGCTGC AAAAACATCA AACATGATTG CCCCTCACCC 
TCCTGTTCTA ACCCTGTCCT GCTTTCTGGA CATTGCTGCA AGACTTGTCC CAAAGCTCCT 
CCTCCTCCTA TAAAGAAATC AGACTTTGTT TTTGATGGAT TCGAGTATTT TCATGGGAAG 
GATGATGATC TTTATAATGA TCGCTCTTAC CTTAGTTCTG ATGATGTCAC TGTGGAGGAG 
AATCGTTCAG AATATGTTGC ATTATTAACA GCACCCAGCC ACGTGTGGCC CCCAGTTACC 
AGTGGAGTAG CCAAGGCTCG ATTCAATCTG CAGCGCTCCA GTTTGCTCTT CTCAATCACC 
TATAAATGGA TAGACAGACT TTCCCGAATC CGCTTCTCCG ATCTTGAAGG TTCTGTGCTG 
TTTGAACACC CAGTGCACAG AATGGGATCC CCACGAGGTG ACACTATCTG TGGTATTTGG 
AGGTCTCTAA ATCGCTCAAC TCTACGTCTT CTCCGCATGG GTCACATTCT GGTATCTTTG 
GTGACCACAA CACTTTCAGA GCCTGAAATA TCAGGCAAGA TTGTCAAACA CAAAGCCCTG 
TTTTCAGAGT CCTTCAGTGC ACTTCTTACC CCAGAAGACT CTGATGTAAC AGGAGGTGGA 
GGACTCGCAA TGCTTACACT AAGTGACGTG GATGACAATC TGCACTTTAT CCTTATGCTC 
AGAGGTCTTC GTGGTGGAGA AAGAGATCAG ATTCAAATAC TTGTGCAAAT CTTGCATCAG 
AACCATGTGA TACGGGAGCT TTACGCTAAC ATCTCTGCAC AGGAACAAGA CTTTGCAGAG 
GTGTTGCCAG ACCTTTCAAG CCGTGAGATG CAGTGGCTGG CCCAAGGTCA GCTGGAGATT 
TCAGTGCAGA CAGAAGGGAG ACGTGGGCAA CGCATGTCAG GCATCATCAC AGTCAGAAAA 
TCATGTGACA CACTGCAGAG TGTGTTGTCT GGTGGTGACG CTTTAAATCC CACCAAAACT 
GGAGCTGTGG GATCTGCAAG CATCACACTG CATGAAAATG GAACCATGGA ATATCAGATT 
CAAATTGCTG GTACCACAAG TGCTGTGACA GCAGTGACAC TGGAGACAAA ACCTCGCAGA 
AAAACAAAGA GAAATATACT GCATGATATG ACCAAGGACT ACCAAGATGG AAGGGTCTGG 
GGATACTGGA TAGATGCTAA TGCACGAGAC CTACATATGC TGTTGCAAAG TGAGCTCTTC 
CTTAATGTAG CAACAAAGGA TTTCCAGGAA GGGGAACTTA GAGGTCAAAT AACCCCTCTG 
CTATACAGCG GCCTTTGGGC CAGATATGAG CAGCTCCCAG TTCCTCTAGC TGGTCAGTTT 
GTGTCACCAC CCATCAGGAC AGGCTCAGCA GGTCACGCAT GGGTTTCACT GGATGAGCAC 
TGCCATCTGC ATTATCAGAT TGTGGTGACT GGTCTGGGTA AGGCAGAGGA TGCTGCACTG 
AATGCTCATC TCCATGGTTT TGCTGAGCTT GGTGAAGTCG GTGAGAGTAC CCCTGGGCAC 
AAGAGGTTGT TAAAGGGCTT CTATGGGTCA GAGGCCCAGG GTAGTGTAAA AGACCTTGAT 
CTTGAACTTT TGGGACATCT GAGCCGGGGC ACAGCATTTA TTCAAGTGAG CACCAAACTG 
AACCCTCGTG GTGAAATTCG AGGACAAGTG CACATACCTA ACAACTGTGA ATCTGGAGGA 
GTCTCTCTAA CTCCCGAGGA GCCTGAGTAT GAATATGAAT TATATGAGGA GGGAAGGCAG 
CGAGACCCAG AAGATCTTCG GAAAGATCCT AGAGCATGCT TTTTTGAAGG TCAACTAAGA 
GCCCATGGTT CACGATGGGC TCCAGATTAT GACAGGAAAT GCTCTGTGTG CAGCTGTCAG 
AAACGTACCG TGATCTGTGA TCCTATTGTG TGCCCACCTC TGAACTGCTC CCAGCCTGTC 
CATTTGTCAG ATCAGTGTTG TCCTGTGTGC GAAGAAAAAA AAGAAATTAG AGAGGTAAAA 
AAAACCGAGA GAGCTCGCAC AGCAAATGAA GGCTGCTTCT TTGATGGAGA TCGCTCATGG 
AAGGCGGCTG GTACACGTTG GCATCCTTTT GTTCCTCCAT TTGGTCTAAT TAAATGTGCT 
ATTTGCACCT GCAAGGGTTC CACTGGAGAA GTGCATTGTG AGAAGGTGAC CTGTCCACGA 
CTTTCCTGTA CCAACCCAAT TCGTGCTAAT CCATCTGATT GCTGCAAACA GTGCCCAGTA 
GAGGAGCGAA GTCCTTTGGA GCTGGCAGAC AGTATGCAGT CAGATGGACC GCGATCATGC 
AAATTTGGGC GTCACTGGTA CCCTAATAAT GAGCGCTGGC ATCCAACTGT GACACCCTTT 
GGAGAGATGA AGTGTGTTAC ATGTGCTTGT GTGGAGGGAG TTACACAGTG TCGGAGACAG 
GAGTGTACAG GAAATACATG TGGTACTGGT TCAAAGCGGG ACAGATGTTG CACCAAGTGC 
AAAGATGCCA AGCAAGATGA AGATGAAAAG GTGAAATCAG AGGAGACAAG GACTCCGTGG 
AGTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chrd.1 ( XM_012962977.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818431.1 Xenopus tropicalis chordin, gene 1 (chrd.1), transcript variant X2, mRNA.

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CloneID	OXa58015
Clone ID Related Accession (Same CDS sequence)	XM_012962977.3
Accession Version	XM_012962977.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chordin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962977.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGTT CCCCCATCTT ACTTCTGTGG ACCCTGTGGA TTATGGCTGT GGATTGCTCT 
CGCCCAAAGG TTTTCCTGCC CATTCAGCCT GAACAGGAGC CCCTCCAATC TAAGACACCA 
GCAGGTTGTA CGTTTGGTGG AAAATTTTAC TCTCTGGAGG ATTCATGGCA TCCAGATCTT 
GGGGATCCAT TCGGAGTTAT GCACTGTGTA TTATGCTATT GTGAGCCGCG AAGTCGCCGG 
GGAAAACCAT CTGGCAAAGT CAGCTGCAAA AACATCAAAC ATGATTGCCC CTCACCCTCC 
TGTTCTAACC CTGTCCTGCT TTCTGGACAT TGCTGCAAGA CTTGTCCCAA AGCTCCTCCT 
CCTCCTATAA AGAAATCAGA CTTTGTTTTT GATGGATTCG AGTATTTTCA TGGGAAGGAT 
GATGATCTTT ATAATGATCG CTCTTACCTT AGTTCTGATG ATGTCACTGT GGAGGAGAAT 
CGTTCAGAAT ATGTTGCATT ATTAACAGCA CCCAGCCACG TGTGGCCCCC AGTTACCAGT 
GGAGTAGCCA AGGCTCGATT CAATCTGCAG CGCTCCAGTT TGCTCTTCTC AATCACCTAT 
AAATGGATAG ACAGACTTTC CCGAATCCGC TTCTCCGATC TTGAAGGTTC TGTGCTGTTT 
GAACACCCAG TGCACAGAAT GGGATCCCCA CGAGGTGACA CTATCTGTGG TATTTGGAGG 
TCTCTAAATC GCTCAACTCT ACGTCTTCTC CGCATGGGTC ACATTCTGGT ATCTTTGGTG 
ACCACAACAC TTTCAGAGCC TGAAATATCA GGCAAGATTG TCAAACACAA AGCCCTGTTT 
TCAGAGTCCT TCAGTGCACT TCTTACCCCA GAAGACTCTG ATGTAACAGG AGGTGGAGGA 
CTCGCAATGC TTACACTAAG TGACGTGGAT GACAATCTGC ACTTTATCCT TATGCTCAGA 
GGTCTTCGTG GTGGAGAAAG AGATCAGATT CAAATACTTG TGCAAATCTT GCATCAGAAC 
CATGTGATAC GGGAGCTTTA CGCTAACATC TCTGCACAGG AACAAGACTT TGCAGAGGTG 
TTGCCAGACC TTTCAAGCCG TGAGATGCAG TGGCTGGCCC AAGGTCAGCT GGAGATTTCA 
GTGCAGACAG AAGGGAGACG TGGGCAACGC ATGTCAGGCA TCATCACAGT CAGAAAATCA 
TGTGACACAC TGCAGAGTGT GTTGTCTGGT GGTGACGCTT TAAATCCCAC CAAAACTGGA 
GCTGTGGGAT CTGCAAGCAT CACACTGCAT GAAAATGGAA CCATGGAATA TCAGATTCAA 
ATTGCTGGTA CCACAAGTGC TGTGACAGCA GTGACACTGG AGACAAAACC TCGCAGAAAA 
ACAAAGAGAA ATATACTGCA TGATATGACC AAGGACTACC AAGATGGAAG GGTCTGGGGA 
TACTGGATAG ATGCTAATGC ACGAGACCTA CATATGCTGT TGCAAAGTGA GCTCTTCCTT 
AATGTAGCAA CAAAGGATTT CCAGGAAGGG GAACTTAGAG GTCAAATAAC CCCTCTGCTA 
TACAGCGGCC TTTGGGCCAG ATATGAGCAG CTCCCAGTTC CTCTAGCTGG TCAGTTTGTG 
TCACCACCCA TCAGGACAGG CTCAGCAGGT CACGCATGGG TTTCACTGGA TGAGCACTGC 
CATCTGCATT ATCAGATTGT GGTGACTGGT CTGGGTAAGG CAGAGGATGC TGCACTGAAT 
GCTCATCTCC ATGGTTTTGC TGAGCTTGGT GAAGTCGGTG AGAGTACCCC TGGGCACAAG 
AGGTTGTTAA AGGGCTTCTA TGGGTCAGAG GCCCAGGGTA GTGTAAAAGA CCTTGATCTT 
GAACTTTTGG GACATCTGAG CCGGGGCACA GCATTTATTC AAGTGAGCAC CAAACTGAAC 
CCTCGTGGTG AAATTCGAGG ACAAGTGCAC ATACCTAACA ACTGTGAATC TGGAGGAGTC 
TCTCTAACTC CCGAGGAGCC TGAGTATGAA TATGAATTAT ATGAGGAGGG AAGGCAGCGA 
GACCCAGAAG ATCTTCGGAA AGATCCTAGA GCATGCTTTT TTGAAGGTCA ACTAAGAGCC 
CATGGTTCAC GATGGGCTCC AGATTATGAC AGGAAATGCT CTGTGTGCAG CTGTCAGAAA 
CGTACCGTGA TCTGTGATCC TATTGTGTGC CCACCTCTGA ACTGCTCCCA GCCTGTCCAT 
TTGTCAGATC AGTGTTGTCC TGTGTGCGAA GAAAAAAAAG AAATTAGAGA GGTAAAAAAA 
ACCGAGAGAG CTCGCACAGC AAATGAAGGC TGCTTCTTTG ATGGAGATCG CTCATGGAAG 
GCGGCTGGTA CACGTTGGCA TCCTTTTGTT CCTCCATTTG GTCTAATTAA ATGTGCTATT 
TGCACCTGCA AGGGTTCCAC TGGAGAAGTG CATTGTGAGA AGGTGACCTG TCCACGACTT 
TCCTGTACCA ACCCAATTCG TGCTAATCCA TCTGATTGCT GCAAACAGTG CCCAGTAGAG 
GAGCGAAGTC CTTTGGAGCT GGCAGACAGT ATGCAGTCAG ATGGACCGCG ATCATGCAAA 
TTTGGGCGTC ACTGGTACCC TAATAATGAG CGCTGGCATC CAACTGTGAC ACCCTTTGGA 
GAGATGAAGT GTGTTACATG TGCTTGTGTG GAGGGAGTTA CACAGTGTCG GAGACAGGAG 
TGTACAGGAA ATACATGTGG TACTGGTTCA AAGCGGGACA GATGTTGCAC CAAGTGCAAA 
GATGCCAAGC AAGATGAAGA TGAAAAGGTG AAATCAGAGG AGACAAGGAC TCCGTGGAGT 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818431.1
CDS	447..3272
Translation	MLCSPILLLWTLWIMAVDCSRPKVFLPIQPEQEPLQSKTPAGCTFGGKFYSLEDSWHPDLGDPFGVMHCVLCYCEPRSRRGKPSGKVSCKNIKHDCPSPSCSNPVLLSGHCCKTCPKAPPPPIKKSDFVFDGFEYFHGKDDDLYNDRSYLSSDDVTVEENRSEYVALLTAPSHVWPPVTSGVAKARFNLQRSSLLFSITYKWIDRLSRIRFSDLEGSVLFEHPVHRMGSPRGDTICGIWRSLNRSTLRLLRMGHILVSLVTTTLSEPEISGKIVKHKALFSESFSALLTPEDSDVTGGGGLAMLTLSDVDDNLHFILMLRGLRGGERDQIQILVQILHQNHVIRELYANISAQEQDFAEVLPDLSSREMQWLAQGQLEISVQTEGRRGQRMSGIITVRKSCDTLQSVLSGGDALNPTKTGAVGSASITLHENGTMEYQIQIAGTTSAVTAVTLETKPRRKTKRNILHDMTKDYQDGRVWGYWIDANARDLHMLLQSELFLNVATKDFQEGELRGQITPLLYSGLWARYEQLPVPLAGQFVSPPIRTGSAGHAWVSLDEHCHLHYQIVVTGLGKAEDAALNAHLHGFAELGEVGESTPGHKRLLKGFYGSEAQGSVKDLDLELLGHLSRGTAFIQVSTKLNPRGEIRGQVHIPNNCESGGVSLTPEEPEYEYELYEEGRQRDPEDLRKDPRACFFEGQLRAHGSRWAPDYDRKCSVCSCQKRTVICDPIVCPPLNCSQPVHLSDQCCPVCEEKKEIREVKKTERARTANEGCFFDGDRSWKAAGTRWHPFVPPFGLIKCAICTCKGSTGEVHCEKVTCPRLSCTNPIRANPSDCCKQCPVEERSPLELADSMQSDGPRSCKFGRHWYPNNERWHPTVTPFGEMKCVTCACVEGVTQCRRQECTGNTCGTGSKRDRCCTKCKDAKQDEDEKVKSEETRTPWSF

Target ORF information:

RefSeq Version	XM_012962977.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chordin, gene 1 (chrd.1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962977.3

ORF Insert Sequence:

ATGCTTTGTT CCCCCATCTT ACTTCTGTGG ACCCTGTGGA TTATGGCTGT GGATTGCTCT 
CGCCCAAAGG TTTTCCTGCC CATTCAGCCT GAACAGGAGC CCCTCCAATC TAAGACACCA 
GCAGGTTGTA CGTTTGGTGG AAAATTTTAC TCTCTGGAGG ATTCATGGCA TCCAGATCTT 
GGGGATCCAT TCGGAGTTAT GCACTGTGTA TTATGCTATT GTGAGCCGCG AAGTCGCCGG 
GGAAAACCAT CTGGCAAAGT CAGCTGCAAA AACATCAAAC ATGATTGCCC CTCACCCTCC 
TGTTCTAACC CTGTCCTGCT TTCTGGACAT TGCTGCAAGA CTTGTCCCAA AGCTCCTCCT 
CCTCCTATAA AGAAATCAGA CTTTGTTTTT GATGGATTCG AGTATTTTCA TGGGAAGGAT 
GATGATCTTT ATAATGATCG CTCTTACCTT AGTTCTGATG ATGTCACTGT GGAGGAGAAT 
CGTTCAGAAT ATGTTGCATT ATTAACAGCA CCCAGCCACG TGTGGCCCCC AGTTACCAGT 
GGAGTAGCCA AGGCTCGATT CAATCTGCAG CGCTCCAGTT TGCTCTTCTC AATCACCTAT 
AAATGGATAG ACAGACTTTC CCGAATCCGC TTCTCCGATC TTGAAGGTTC TGTGCTGTTT 
GAACACCCAG TGCACAGAAT GGGATCCCCA CGAGGTGACA CTATCTGTGG TATTTGGAGG 
TCTCTAAATC GCTCAACTCT ACGTCTTCTC CGCATGGGTC ACATTCTGGT ATCTTTGGTG 
ACCACAACAC TTTCAGAGCC TGAAATATCA GGCAAGATTG TCAAACACAA AGCCCTGTTT 
TCAGAGTCCT TCAGTGCACT TCTTACCCCA GAAGACTCTG ATGTAACAGG AGGTGGAGGA 
CTCGCAATGC TTACACTAAG TGACGTGGAT GACAATCTGC ACTTTATCCT TATGCTCAGA 
GGTCTTCGTG GTGGAGAAAG AGATCAGATT CAAATACTTG TGCAAATCTT GCATCAGAAC 
CATGTGATAC GGGAGCTTTA CGCTAACATC TCTGCACAGG AACAAGACTT TGCAGAGGTG 
TTGCCAGACC TTTCAAGCCG TGAGATGCAG TGGCTGGCCC AAGGTCAGCT GGAGATTTCA 
GTGCAGACAG AAGGGAGACG TGGGCAACGC ATGTCAGGCA TCATCACAGT CAGAAAATCA 
TGTGACACAC TGCAGAGTGT GTTGTCTGGT GGTGACGCTT TAAATCCCAC CAAAACTGGA 
GCTGTGGGAT CTGCAAGCAT CACACTGCAT GAAAATGGAA CCATGGAATA TCAGATTCAA 
ATTGCTGGTA CCACAAGTGC TGTGACAGCA GTGACACTGG AGACAAAACC TCGCAGAAAA 
ACAAAGAGAA ATATACTGCA TGATATGACC AAGGACTACC AAGATGGAAG GGTCTGGGGA 
TACTGGATAG ATGCTAATGC ACGAGACCTA CATATGCTGT TGCAAAGTGA GCTCTTCCTT 
AATGTAGCAA CAAAGGATTT CCAGGAAGGG GAACTTAGAG GTCAAATAAC CCCTCTGCTA 
TACAGCGGCC TTTGGGCCAG ATATGAGCAG CTCCCAGTTC CTCTAGCTGG TCAGTTTGTG 
TCACCACCCA TCAGGACAGG CTCAGCAGGT CACGCATGGG TTTCACTGGA TGAGCACTGC 
CATCTGCATT ATCAGATTGT GGTGACTGGT CTGGGTAAGG CAGAGGATGC TGCACTGAAT 
GCTCATCTCC ATGGTTTTGC TGAGCTTGGT GAAGTCGGTG AGAGTACCCC TGGGCACAAG 
AGGTTGTTAA AGGGCTTCTA TGGGTCAGAG GCCCAGGGTA GTGTAAAAGA CCTTGATCTT 
GAACTTTTGG GACATCTGAG CCGGGGCACA GCATTTATTC AAGTGAGCAC CAAACTGAAC 
CCTCGTGGTG AAATTCGAGG ACAAGTGCAC ATACCTAACA ACTGTGAATC TGGAGGAGTC 
TCTCTAACTC CCGAGGAGCC TGAGTATGAA TATGAATTAT ATGAGGAGGG AAGGCAGCGA 
GACCCAGAAG ATCTTCGGAA AGATCCTAGA GCATGCTTTT TTGAAGGTCA ACTAAGAGCC 
CATGGTTCAC GATGGGCTCC AGATTATGAC AGGAAATGCT CTGTGTGCAG CTGTCAGAAA 
CGTACCGTGA TCTGTGATCC TATTGTGTGC CCACCTCTGA ACTGCTCCCA GCCTGTCCAT 
TTGTCAGATC AGTGTTGTCC TGTGTGCGAA GAAAAAAAAG AAATTAGAGA GGTAAAAAAA 
ACCGAGAGAG CTCGCACAGC AAATGAAGGC TGCTTCTTTG ATGGAGATCG CTCATGGAAG 
GCGGCTGGTA CACGTTGGCA TCCTTTTGTT CCTCCATTTG GTCTAATTAA ATGTGCTATT 
TGCACCTGCA AGGGTTCCAC TGGAGAAGTG CATTGTGAGA AGGTGACCTG TCCACGACTT 
TCCTGTACCA ACCCAATTCG TGCTAATCCA TCTGATTGCT GCAAACAGTG CCCAGTAGAG 
GAGCGAAGTC CTTTGGAGCT GGCAGACAGT ATGCAGTCAG ATGGACCGCG ATCATGCAAA 
TTTGGGCGTC ACTGGTACCC TAATAATGAG CGCTGGCATC CAACTGTGAC ACCCTTTGGA 
GAGATGAAGT GTGTTACATG TGCTTGTGTG GAGGGAGTTA CACAGTGTCG GAGACAGGAG 
TGTACAGGAA ATACATGTGG TACTGGTTCA AAGCGGGACA GATGTTGCAC CAAGTGCAAA 
GATGCCAAGC AAGATGAAGA TGAAAAGGTG AAATCAGAGG AGACAAGGAC TCCGTGGAGT 
TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Twisted gastrulation is required for forebrain specification and cooperates with Chordin to inhibit BMP signaling during X. tropicalis gastrulation.
Developmental biology289(1)166-78(2006 Jan)
Wills A,Harland RM,Khokha MK

The novel Smad-interacting protein Smicl regulates Chordin expression in the Xenopus embryo.
Development (Cambridge, England)132(20)4575-86(2005 Oct)
Collart C,Verschueren K,Rana A,Smith JC,Huylebroeck D

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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chrd.1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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