CHRD cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRD
Entrez Gene ID	395828
Full Name	chordin
General protein information	Preferred Names chordin Names chordin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015291373.2	XP_015146859.1	chordin isoform X2
XM_015291372.2	XP_015146858.1	chordin isoform X1
XM_025153315.1	XP_025009083.1	chordin isoform X4
XM_015291374.1	XP_015146860.1	chordin isoform X3
XM_015291374.2	XP_015146860.1	chordin isoform X3
XM_015291372.1	XP_015146858.1	chordin isoform X1
XM_015291373.1	XP_015146859.1	chordin isoform X2
NM_204980.2	NP_990311.2	chordin precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHRD	XP_005639840.1
Danio rerio (zebrafish)	chd	NP_571048.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000472	XP_310620.4
Bos taurus (cattle)	CHRD	XP_002684938.2
Drosophila melanogaster (fruit fly)	sog	NP_001259578.1
Homo sapiens (human)	CHRD	NP_003732.2
Mus musculus (house mouse)	Chrd	NP_001264970.1
Gallus gallus (chicken)	CHRD	NP_990311.1
Pan troglodytes (chimpanzee)	CHRD	XP_516917.3
	CHRD	XP_001097729.2
Rattus norvegicus (Norway rat)	Chrd	NP_476475.1
Xenopus tropicalis (tropical clawed frog)	chrd	NP_001136129.1

Gene Ontology
Bibliography

Related articles in PubMed

Chordin regulates primitive streak development and the stability of induced neural cells, but is not sufficient for neural induction in the chick embryo.
Streit A, Lee KJ, Woo I, Roberts C, Jessell TM, Stern CD
Development (Cambridge, England)125(3)507-19(1998 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHRD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa28387	NM_204980.2 Latest version!	Gallus gallus chordin (CHRD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
OGa152626	XM_025153315.1 Latest version!	Gallus gallus chordin (CHRD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa39870	XM_015291372.2 Latest version!	Gallus gallus chordin (CHRD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa39871	XM_015291373.2 Latest version!	Gallus gallus chordin (CHRD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa39872	XM_015291374.2 Latest version!	Gallus gallus chordin (CHRD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
OGa39872	XM_015291374.1	Gallus gallus chordin (CHRD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa39870	XM_015291372.1	Gallus gallus chordin (CHRD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OGa39871	XM_015291373.1	Gallus gallus chordin (CHRD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRD ( NM_204980.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990311.2 Gallus gallus chordin (CHRD), mRNA.

Price & Availability↑

CloneID	OGa28387
Clone ID Related Accession (Same CDS sequence)	NM_204980.2
Accession Version	NM_204980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-25
Organism	Gallus gallus(chicken)
Product	chordin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000526.1. On Mar 18, 2015 this sequence version replaced NM_204980.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF031230.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

ATGCGCACCG CCCTGCTGCT GCTCGCCCTG CTCGCCCTGC CCGTCCGCAC CGCCCGCCCC 
AAGCTCGCCC TGCCCATCCG GCCCGACAGC GAGCCGCTGC CCCCCGGGGG GGCGGCAGGT 
TGTGCCTTCG GGGGACGTTT CTACGCGCTG GAGGAGACGT GGCACCCCGA CCTGGGGGAG 
CCCTTTGGGG TGATGCGCTG CGTGATCTGC CACTGCGAGA CGCAGAGGAA TCGCCGAGGG 
AAACCAGTGG GGAAAGTGAA CTGCAAGAAC ATGAAGCAGG ATTGCCCCGT TCCCACCTGC 
CCCCGTGCCA CGCTGTTGCC CGGGCACTGC TGCCATACCT GTCCCAAAGC CTTGCCAGGA 
GCCCCGGAGA AGAGCTACAA GCCACCTTTT GACACCTTTG AGTACTTCCA GGACAAGGAG 
GACGAGCTGG ACAAGCCGTA CAATGACCGC TCCTACCTCA GCTCTGAGGG TTCGGCACGT 
GACGATGCCC GCACAGAATT CGTGGCCCTG CTGACGAGCG GCCCCGAGCC GTGGCACCCC 
ACATCCAGCG CGGTGGCCAA GGCTCGCTTC ACCCTGCTGC GCTCCTACCT GCTCTTCTCC 
ATCAGCTACG AGCGGCTGGG CCGGCCGAGC CGTGTGCGTT TCAGTGACCC CGAGGGCACC 
GTGCTGTTCG AGCACCCAGT GCAGAAGAGC GCAGCCCCTG AGGACGGCAT GCTCTGTGGG 
ATGTGGAGGA CAGTGTCCAA AGCCAACATA CAGCTGCTGC GTGCGGAGCA GCTCCGTGTG 
TCCCTCATCA CCCGTGCGCA GCCCTCCGGA GAGGTGCATG GGCACATCCT GAAGCACCGG 
GCGCTCTTTG CAGAGACATT TGGTGCCATC CTGACCTCAT CAGACCCCGC GCACCTGGGG 
GCCGGGGGCA TGGCCATGCT GACGCTGAGC GACACCGAGA ACAACCTACA CTTCATCCTC 
ATGGCCCGTG GGCTGCTGGA GCCCGGTGCT GAGGAGTCCC CCTGGGTACC GCTGCGGGTC 
CGCATCCTGC ACCAGGGCCA GACGCTGCGC GAGGCCCATG CCAACATCAC TATGGAGGAC 
CCCGACTTCG CGGAGGTACT GAGCGACCTG TCTGCCCATG AGCTGCAGTG GTTGGCACAG 
GGGCAGCTCC GCATCGTGGC TGACACCCAG GGCCGGCACC CGCGCCAGCT GGAGGGCACC 
ATTACCGCCC GCCGCAGCTG TGACACCATC CAAAGCGTGC TGTGCGGCGC AGATGCATTG 
CAGCCCACCA AGACGGGGGC GGTGGGCTCG GCCAAGCTGG CGCTGCATGA GAACGGCACG 
CTGGAGTACC AGGTGCGGGT GGTGGGCACG GCCAGCGAGG TGGTGGGGGT CACACTGGAG 
ACCAAACCGC GGCGGAAGAA CAGGAGGAAC GTCCTCTTCG ACATGACACC CAGCTATAGG 
GCTGGGATGG CTCAAGGAGT GTGGCACAGC CCCAGCGCCC GCGATGTGCA CATGCTGCTG 
CAGAATGAGC TCTTCCTCAA CGTGGCCACC AAGGACTGGG CAGAGGGAGA GCTGCGGGGC 
CAGGTCATCT CCCTGCCCTA CAGTGGGCTC CTGGCACGCT ACACAGAGAT GCCGGTGCCG 
CTGGCAGGGC AGCTGGTATC CCCCCCAGTG AGCAGCGGGG CCGGGGGCCA CGCGTGGCTG 
TCACTGGATG AGCACTGCCA CCTGCACTAC GAGATAGTGG TGGCGGGTCT GGGCCGCCCG 
GCCGATGGCA CAGTCAGTGC CCAGCTGCAC GGCGTGGCCG AGCTGGGCGA GATGGGCACT 
CGGCCGCACC TGCACAAGCG GATGCTCAAG GGGTTCTATG GCACTGAGGC TCAGGGGGTG 
GTGAAGGACC TGGATGCTGA GCTGCTGCAG CACCTGGCAC AGGGCACCGC ATTCCTGCAA 
GTCAGCACCA AAGCACACCC GCGTGGGGAG ATGCGGGGAT GGGTGCACAT CCCCAACCGC 
TGTCAGCCAG GAGGGGCCCG CCTGTCCACC GGGGAGGCTG AGCTCTCAGA GGGCCCTAAG 
GGCAGGAATG TGGAACAGCT GAAAAAGGAC CCCAACTCCT GCTTCTTCGA GGGGCAGCAC 
CATGCACACG GCACCCGCTG GGCGCCTGAC TACGACAAGA AGTGCTCCAT CTGCAGCTGC 
CAGAAGCGCA CGGTCATCTG CGATCCCATC CTGTGCCAGC CCCTCAACTG TACCCGCCAG 
GTGCACCCTG AGGAGCTGTG CTGCCCCATC TGTGAAGAGA AGAAGATGGA GCAGGAGGAG 
CTGAAGCTGG AACGGGCACG GGACAGCAGC GAGGGCTGCT ACTTTGATGG GGACAAGACA 
TGGCGAGGCT CTGGCACCCG CTGGCACCCT GTCGTGCCGC CGTTTGGCCT CATCAAATGT 
GCCATCTGCA CCTGCAAGGG CACCACGGGT GAAGTGCACT GCGAGAAGGT GCAGTGTCCG 
CGCCTCACCT GTGCCAACCC TGTGCGCGTC AGCCCCTCCG ACTGCTGCAA GCAGTGCCCA 
GCCCCTGAGA AGAGCATCCC TGAGCTGACT GATGGCATGC AGGCGGACGG ACCCCGTGCG 
TGCCGCTTTG GGCGCCGCTG GTACCTCAAC AACGAGAGCT GGCACCCCTC AGTGCCACCC 
TTTGGGGAGA TGAAGTGCAT CCTGTGCTGG TGTGTGTCAG GAGAGACCCA CTGCCAGCGC 
CAGGAGTGCC CGCCAGATGC CTGTGCCAGC CCCACCAGGA GGGACAACCC CTGCTGTGCC 
AAGTGTCGCG CTCCGGATGC ACCCTCAGAA AAGATGTACG AAGCCACAGC AGAGGCATGG 
AGCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990311.2
CDS	1..2829
Translation	MRTALLLLALLALPVRTARPKLALPIRPDSEPLPPGGAAGCAFGGRFYALEETWHPDLGEPFGVMRCVICHCETQRNRRGKPVGKVNCKNMKQDCPVPTCPRATLLPGHCCHTCPKALPGAPEKSYKPPFDTFEYFQDKEDELDKPYNDRSYLSSEGSARDDARTEFVALLTSGPEPWHPTSSAVAKARFTLLRSYLLFSISYERLGRPSRVRFSDPEGTVLFEHPVQKSAAPEDGMLCGMWRTVSKANIQLLRAEQLRVSLITRAQPSGEVHGHILKHRALFAETFGAILTSSDPAHLGAGGMAMLTLSDTENNLHFILMARGLLEPGAEESPWVPLRVRILHQGQTLREAHANITMEDPDFAEVLSDLSAHELQWLAQGQLRIVADTQGRHPRQLEGTITARRSCDTIQSVLCGADALQPTKTGAVGSAKLALHENGTLEYQVRVVGTASEVVGVTLETKPRRKNRRNVLFDMTPSYRAGMAQGVWHSPSARDVHMLLQNELFLNVATKDWAEGELRGQVISLPYSGLLARYTEMPVPLAGQLVSPPVSSGAGGHAWLSLDEHCHLHYEIVVAGLGRPADGTVSAQLHGVAELGEMGTRPHLHKRMLKGFYGTEAQGVVKDLDAELLQHLAQGTAFLQVSTKAHPRGEMRGWVHIPNRCQPGGARLSTGEAELSEGPKGRNVEQLKKDPNSCFFEGQHHAHGTRWAPDYDKKCSICSCQKRTVICDPILCQPLNCTRQVHPEELCCPICEEKKMEQEELKLERARDSSEGCYFDGDKTWRGSGTRWHPVVPPFGLIKCAICTCKGTTGEVHCEKVQCPRLTCANPVRVSPSDCCKQCPAPEKSIPELTDGMQADGPRACRFGRRWYLNNESWHPSVPPFGEMKCILCWCVSGETHCQRQECPPDACASPTRRDNPCCAKCRAPDAPSEKMYEATAEAWSR

Target ORF information:

RefSeq Version	NM_204980.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chordin (CHRD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204980.2

ORF Insert Sequence:

ATGCGCACCG CCCTGCTGCT GCTCGCCCTG CTCGCCCTGC CCGTCCGCAC CGCCCGCCCC 
AAGCTCGCCC TGCCCATCCG GCCCGACAGC GAGCCGCTGC CCCCCGGGGG GGCGGCAGGT 
TGTGCCTTCG GGGGACGTTT CTACGCGCTG GAGGAGACGT GGCACCCCGA CCTGGGGGAG 
CCCTTTGGGG TGATGCGCTG CGTGATCTGC CACTGCGAGA CGCAGAGGAA TCGCCGAGGG 
AAACCAGTGG GGAAAGTGAA CTGCAAGAAC ATGAAGCAGG ATTGCCCCGT TCCCACCTGC 
CCCCGTGCCA CGCTGTTGCC CGGGCACTGC TGCCATACCT GTCCCAAAGC CTTGCCAGGA 
GCCCCGGAGA AGAGCTACAA GCCACCTTTT GACACCTTTG AGTACTTCCA GGACAAGGAG 
GACGAGCTGG ACAAGCCGTA CAATGACCGC TCCTACCTCA GCTCTGAGGG TTCGGCACGT 
GACGATGCCC GCACAGAATT CGTGGCCCTG CTGACGAGCG GCCCCGAGCC GTGGCACCCC 
ACATCCAGCG CGGTGGCCAA GGCTCGCTTC ACCCTGCTGC GCTCCTACCT GCTCTTCTCC 
ATCAGCTACG AGCGGCTGGG CCGGCCGAGC CGTGTGCGTT TCAGTGACCC CGAGGGCACC 
GTGCTGTTCG AGCACCCAGT GCAGAAGAGC GCAGCCCCTG AGGACGGCAT GCTCTGTGGG 
ATGTGGAGGA CAGTGTCCAA AGCCAACATA CAGCTGCTGC GTGCGGAGCA GCTCCGTGTG 
TCCCTCATCA CCCGTGCGCA GCCCTCCGGA GAGGTGCATG GGCACATCCT GAAGCACCGG 
GCGCTCTTTG CAGAGACATT TGGTGCCATC CTGACCTCAT CAGACCCCGC GCACCTGGGG 
GCCGGGGGCA TGGCCATGCT GACGCTGAGC GACACCGAGA ACAACCTACA CTTCATCCTC 
ATGGCCCGTG GGCTGCTGGA GCCCGGTGCT GAGGAGTCCC CCTGGGTACC GCTGCGGGTC 
CGCATCCTGC ACCAGGGCCA GACGCTGCGC GAGGCCCATG CCAACATCAC TATGGAGGAC 
CCCGACTTCG CGGAGGTACT GAGCGACCTG TCTGCCCATG AGCTGCAGTG GTTGGCACAG 
GGGCAGCTCC GCATCGTGGC TGACACCCAG GGCCGGCACC CGCGCCAGCT GGAGGGCACC 
ATTACCGCCC GCCGCAGCTG TGACACCATC CAAAGCGTGC TGTGCGGCGC AGATGCATTG 
CAGCCCACCA AGACGGGGGC GGTGGGCTCG GCCAAGCTGG CGCTGCATGA GAACGGCACG 
CTGGAGTACC AGGTGCGGGT GGTGGGCACG GCCAGCGAGG TGGTGGGGGT CACACTGGAG 
ACCAAACCGC GGCGGAAGAA CAGGAGGAAC GTCCTCTTCG ACATGACACC CAGCTATAGG 
GCTGGGATGG CTCAAGGAGT GTGGCACAGC CCCAGCGCCC GCGATGTGCA CATGCTGCTG 
CAGAATGAGC TCTTCCTCAA CGTGGCCACC AAGGACTGGG CAGAGGGAGA GCTGCGGGGC 
CAGGTCATCT CCCTGCCCTA CAGTGGGCTC CTGGCACGCT ACACAGAGAT GCCGGTGCCG 
CTGGCAGGGC AGCTGGTATC CCCCCCAGTG AGCAGCGGGG CCGGGGGCCA CGCGTGGCTG 
TCACTGGATG AGCACTGCCA CCTGCACTAC GAGATAGTGG TGGCGGGTCT GGGCCGCCCG 
GCCGATGGCA CAGTCAGTGC CCAGCTGCAC GGCGTGGCCG AGCTGGGCGA GATGGGCACT 
CGGCCGCACC TGCACAAGCG GATGCTCAAG GGGTTCTATG GCACTGAGGC TCAGGGGGTG 
GTGAAGGACC TGGATGCTGA GCTGCTGCAG CACCTGGCAC AGGGCACCGC ATTCCTGCAA 
GTCAGCACCA AAGCACACCC GCGTGGGGAG ATGCGGGGAT GGGTGCACAT CCCCAACCGC 
TGTCAGCCAG GAGGGGCCCG CCTGTCCACC GGGGAGGCTG AGCTCTCAGA GGGCCCTAAG 
GGCAGGAATG TGGAACAGCT GAAAAAGGAC CCCAACTCCT GCTTCTTCGA GGGGCAGCAC 
CATGCACACG GCACCCGCTG GGCGCCTGAC TACGACAAGA AGTGCTCCAT CTGCAGCTGC 
CAGAAGCGCA CGGTCATCTG CGATCCCATC CTGTGCCAGC CCCTCAACTG TACCCGCCAG 
GTGCACCCTG AGGAGCTGTG CTGCCCCATC TGTGAAGAGA AGAAGATGGA GCAGGAGGAG 
CTGAAGCTGG AACGGGCACG GGACAGCAGC GAGGGCTGCT ACTTTGATGG GGACAAGACA 
TGGCGAGGCT CTGGCACCCG CTGGCACCCT GTCGTGCCGC CGTTTGGCCT CATCAAATGT 
GCCATCTGCA CCTGCAAGGG CACCACGGGT GAAGTGCACT GCGAGAAGGT GCAGTGTCCG 
CGCCTCACCT GTGCCAACCC TGTGCGCGTC AGCCCCTCCG ACTGCTGCAA GCAGTGCCCA 
GCCCCTGAGA AGAGCATCCC TGAGCTGACT GATGGCATGC AGGCGGACGG ACCCCGTGCG 
TGCCGCTTTG GGCGCCGCTG GTACCTCAAC AACGAGAGCT GGCACCCCTC AGTGCCACCC 
TTTGGGGAGA TGAAGTGCAT CCTGTGCTGG TGTGTGTCAG GAGAGACCCA CTGCCAGCGC 
CAGGAGTGCC CGCCAGATGC CTGTGCCAGC CCCACCAGGA GGGACAACCC CTGCTGTGCC 
AAGTGTCGCG CTCCGGATGC ACCCTCAGAA AAGATGTACG AAGCCACAGC AGAGGCATGG 
AGCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRD ( XM_025153315.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009083.1 Gallus gallus chordin (CHRD), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa152626
Clone ID Related Accession (Same CDS sequence)	XM_025153315.1
Accession Version	XM_025153315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	chordin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTGA AGAGCAGGAT CCGTGCTGGG TGCTGTTCCC ATACCCTCGG CCGCGGCACC 
TCCCTGGGAC CCCAGGTCTT GTCCCCACGC TCGTGGGAAC GGTCCTGTGG TCCTGCTCTG 
GGATTGCCAT CTGCACCAGG ATTTGGGGAT GCCAAAGGCA CATGTTGTGG GGGCTCGCAG 
CTCCCGCTTG CCCATGCCGC CGCCTATAAA GCGGGGCCGG GGCCGGCGGC CGGGCAGAGG 
CGGTGGCAGC GGAGGCAGCA GGCAGCGCCC GGCCCCCCCT CCCCGCCCCC GGCCGCCCGG 
TTCCCCCGGT TCCCCGGCAC CATGCGCACC GCCCTGCTGC TGCTCGCCCT GCTCGCCCTG 
CCCGTCCGCA CCGCCCGCCC CAAGCTCGCC CTGCCCATCC GGCCCGACAG CGAGCCGCTG 
CCCCCCGGGG GGGCGGCAGG TTGTGCCTTC GGGGGACGTT TCTACGCGCT GGAGGAGACG 
TGGCACCCCG ACCTGGGGGA GCCCTTTGGG GTGATGCGCT GCGTGATCTG CCACTGCGAG 
ACGCAGAGGA ATCGCCGAGG GAAACCAGTG GGGAAAGTGA ACTGCAAGAA CATGAAGCAG 
GATTGCCCCG TTCCCACCTG CCCCCGTGCC ACGCTGTTGC CCGGGCACTG CTGCCATACC 
TGTCCCAAAG CCTTGCCAGG AGCCCCGGAG AAGAGCTACA AGCCACCTTT TGACACCTTT 
GAGTACTTCC AGGACAAGGA GGACGAGCTG GACAAGCCGT ACAATGACCG CTCCTACCTC 
AGCTCTGAGG GTTCGGCACG TGACGATGCC CGCACAGAAT TCGTGGCCCT GCTGACGAGC 
GGCCCCGAGC CGTGGCACCC CACATCCAGC GCGGTGGCCA AGGCTCGCTT CACCCTGCTG 
CGCTCCTACC TGCTCTTCTC CATCAGCTAC GAGCGGCTGG GCCGGCCGAG CCGTGTGCGT 
TTCAGTGACC CCGAGGGCAC CGTGCTGTTC GAGCACCCAG TGCAGAAGAG CGCAGCCCCT 
GAGGACGGCA TGCTCTGTGG GATGTGGAGG ACAGTGTCCA AAGCCAACAT ACAGCTGCTG 
CGTGCGGAGC AGCTCCGTGT GTCCCTCATC ACCCGTGCGC AGCCCTCCGG AGAGGTGCAT 
GGGCACATCC TGAAGCACCG GGCGCTCTTT GCAGAGACAT TTGGTGCCAT CCTGACCTCA 
TCAGACCCCG CGCACCTGGG GGCCGGGGGC ATGGCCATGC TGACGCTGAG CGACACCGAG 
AACAACCTAC ACTTCATCCT CATGGCCCGT GGGCTGCTGG AGCCCGGTGC TGAGGGTGAG 
GGTGGGCATG GGCGCCTGCA AGGCATGGCA TCACCAGTGG GGGGCTCAAG GGAGTCCCCC 
TGGGTACCGC TGCGGGTCCG CATCCTGCAC CAGGGCCAGA CGCTGCGCGA GGCCCATGCC 
AACATCACTA TGGAGGACCC CGACTTCGCG GAGGTACTGA GCGACCTGTC TGCCCATGAG 
CTGCAGTGGT TGGCACAGGG GCAGCTCCGC ATCGTGGCTG ACACCCAGGG CCGGCACCCG 
CGCCAGCTGG AGGGCACCAT TACCGCCCGC CGCAGCTGTG ACACCATCCA AAGCGTGCTG 
TGCGGCGCAG ATGCATTGCA GCCCACCAAG ACGGGGGCGG TGGGCTCGGC CAAGCTGGCG 
CTGCATGAGA ACGGCACGCT GGAGTACCAG GTGCGGGTGG TGGGCACGGC CAGCGAGGTG 
GTGGGGGTCA CACTGGAGAC CAAACCGCGG CGGAAGAACA GGAGGAACGT CCTCTTCGAC 
ATGACACCCA GCTATAGGGC TGGGATGGCT CAAGGAGTGT GGCACAGCCC CAGCGCCCGC 
GATGTGCACA TGCTGCTGCA GAATGAGCTC TTCCTCAACG TGGCCACCAA GGACTGGGCA 
GAGGGAGAGC TGCGGGGCCA GGTCATCTCC CTGCCCTACA GTGGGCTCCT GGCACGCTAC 
ACAGAGATGC CGGTGCCGCT GGCAGGGCAG CTGGTATCCC CCCCAGTGAG CAGCGGGGCC 
GGGGGCCACG CGTGGCTGTC ACTGGATGAG CACTGCCACC TGCACTACGA GATAGTGGTG 
GCGGGTCTGG GCCGCCCGGC CGATGGCACA GTCAGTGCCC AGCTGCACGG CGTGGCCGAG 
CTGGGCGAGA TGGGCACTCG GCCGCACCTG CACAAGCGGA TGCTCAAGGG GTTCTATGGC 
ACTGAGGCTC AGGGGGTGGT GAAGGACCTG GATGCTGAGC TGCTGCAGCA CCTGGCACAG 
GGCACCGCAT TCCTGCAAGT CAGCACCAAA GCACACCCGC GTGGGGAGAT GCGGGGATGG 
GTGCACATCC CCAACCGCTG TCAGCCAGGA GGGGCCCGCC TGTCCACCGG GGAGGCTGAG 
CTCTCAGAGG GCCCTAAGGG CAGGAATGTG GAACAGCTGA AAAAGGACCC CAACTCCTGC 
TTCTTCGAGG GGCAGCACCA TGCACACGGC ACCCGCTGGG CGCCTGACTA CGACAAGAAG 
TGCTCCATCT GCAGCTGCCA GAAGCGCACG GTCATCTGCG ATCCCATCCT GTGCCAGCCC 
CTCAACTGTA CCCGCCAGGT GCACCCTGAG GAGCTGTGCT GCCCCATCTG TGAAGAGAAG 
AAGATGGAGC AGGAGGAGCT GAAGCTGGAA CGGGCACGGG ACAGCAGCGA GGGCTGCTAC 
TTTGATGGGG ACAAGACATG GCGAGGCTCT GGCACCCGCT GGCACCCTGT CGTGCCGCCG 
TTTGGCCTCA TCAAATGTGC CATCTGCACC TGCAAGGGCA CCACGGGTGA AGTGCACTGC 
GAGAAGGTGC AGTGTCCGCG CCTCACCTGT GCCAACCCTG TGCGCGTCAG CCCCTCCGAC 
TGCTGCAAGC AGTGCCCAGC CCCTGAGAAG AGCATCCCTG AGCTGACTGA TGGCATGCAG 
GCGGACGGAC CCCGTGCGTG CCGCTTTGGG CGCCGCTGGT ACCTCAACAA CGAGAGCTGG 
CACCCCTCAG TGCCACCCTT TGGGGAGATG AAGTGCATCC TGTGCTGGTG TGTGTCAGGA 
GAGACCCACT GCCAGCGCCA GGAGTGCCCG CCAGATGCCT GTGCCAGCCC CACCAGGAGG 
GACAACCCCT GCTGTGCCAA GTGTCGCGCT CCGGATGCAC CCTCAGAAAA GATGTACGAA 
GCCACAGCAG AGGCATGGAG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009083.1
CDS	183..3389
Translation	MWVKSRIRAGCCSHTLGRGTSLGPQVLSPRSWERSCGPALGLPSAPGFGDAKGTCCGGSQLPLAHAAAYKAGPGPAAGQRRWQRRQQAAPGPPSPPPAARFPRFPGTMRTALLLLALLALPVRTARPKLALPIRPDSEPLPPGGAAGCAFGGRFYALEETWHPDLGEPFGVMRCVICHCETQRNRRGKPVGKVNCKNMKQDCPVPTCPRATLLPGHCCHTCPKALPGAPEKSYKPPFDTFEYFQDKEDELDKPYNDRSYLSSEGSARDDARTEFVALLTSGPEPWHPTSSAVAKARFTLLRSYLLFSISYERLGRPSRVRFSDPEGTVLFEHPVQKSAAPEDGMLCGMWRTVSKANIQLLRAEQLRVSLITRAQPSGEVHGHILKHRALFAETFGAILTSSDPAHLGAGGMAMLTLSDTENNLHFILMARGLLEPGAEGEGGHGRLQGMASPVGGSRESPWVPLRVRILHQGQTLREAHANITMEDPDFAEVLSDLSAHELQWLAQGQLRIVADTQGRHPRQLEGTITARRSCDTIQSVLCGADALQPTKTGAVGSAKLALHENGTLEYQVRVVGTASEVVGVTLETKPRRKNRRNVLFDMTPSYRAGMAQGVWHSPSARDVHMLLQNELFLNVATKDWAEGELRGQVISLPYSGLLARYTEMPVPLAGQLVSPPVSSGAGGHAWLSLDEHCHLHYEIVVAGLGRPADGTVSAQLHGVAELGEMGTRPHLHKRMLKGFYGTEAQGVVKDLDAELLQHLAQGTAFLQVSTKAHPRGEMRGWVHIPNRCQPGGARLSTGEAELSEGPKGRNVEQLKKDPNSCFFEGQHHAHGTRWAPDYDKKCSICSCQKRTVICDPILCQPLNCTRQVHPEELCCPICEEKKMEQEELKLERARDSSEGCYFDGDKTWRGSGTRWHPVVPPFGLIKCAICTCKGTTGEVHCEKVQCPRLTCANPVRVSPSDCCKQCPAPEKSIPELTDGMQADGPRACRFGRRWYLNNESWHPSVPPFGEMKCILCWCVSGETHCQRQECPPDACASPTRRDNPCCAKCRAPDAPSEKMYEATAEAWSR

Target ORF information:

RefSeq Version	XM_025153315.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chordin (CHRD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153315.1

ORF Insert Sequence:

ATGTGGGTGA AGAGCAGGAT CCGTGCTGGG TGCTGTTCCC ATACCCTCGG CCGCGGCACC 
TCCCTGGGAC CCCAGGTCTT GTCCCCACGC TCGTGGGAAC GGTCCTGTGG TCCTGCTCTG 
GGATTGCCAT CTGCACCAGG ATTTGGGGAT GCCAAAGGCA CATGTTGTGG GGGCTCGCAG 
CTCCCGCTTG CCCATGCCGC CGCCTATAAA GCGGGGCCGG GGCCGGCGGC CGGGCAGAGG 
CGGTGGCAGC GGAGGCAGCA GGCAGCGCCC GGCCCCCCCT CCCCGCCCCC GGCCGCCCGG 
TTCCCCCGGT TCCCCGGCAC CATGCGCACC GCCCTGCTGC TGCTCGCCCT GCTCGCCCTG 
CCCGTCCGCA CCGCCCGCCC CAAGCTCGCC CTGCCCATCC GGCCCGACAG CGAGCCGCTG 
CCCCCCGGGG GGGCGGCAGG TTGTGCCTTC GGGGGACGTT TCTACGCGCT GGAGGAGACG 
TGGCACCCCG ACCTGGGGGA GCCCTTTGGG GTGATGCGCT GCGTGATCTG CCACTGCGAG 
ACGCAGAGGA ATCGCCGAGG GAAACCAGTG GGGAAAGTGA ACTGCAAGAA CATGAAGCAG 
GATTGCCCCG TTCCCACCTG CCCCCGTGCC ACGCTGTTGC CCGGGCACTG CTGCCATACC 
TGTCCCAAAG CCTTGCCAGG AGCCCCGGAG AAGAGCTACA AGCCACCTTT TGACACCTTT 
GAGTACTTCC AGGACAAGGA GGACGAGCTG GACAAGCCGT ACAATGACCG CTCCTACCTC 
AGCTCTGAGG GTTCGGCACG TGACGATGCC CGCACAGAAT TCGTGGCCCT GCTGACGAGC 
GGCCCCGAGC CGTGGCACCC CACATCCAGC GCGGTGGCCA AGGCTCGCTT CACCCTGCTG 
CGCTCCTACC TGCTCTTCTC CATCAGCTAC GAGCGGCTGG GCCGGCCGAG CCGTGTGCGT 
TTCAGTGACC CCGAGGGCAC CGTGCTGTTC GAGCACCCAG TGCAGAAGAG CGCAGCCCCT 
GAGGACGGCA TGCTCTGTGG GATGTGGAGG ACAGTGTCCA AAGCCAACAT ACAGCTGCTG 
CGTGCGGAGC AGCTCCGTGT GTCCCTCATC ACCCGTGCGC AGCCCTCCGG AGAGGTGCAT 
GGGCACATCC TGAAGCACCG GGCGCTCTTT GCAGAGACAT TTGGTGCCAT CCTGACCTCA 
TCAGACCCCG CGCACCTGGG GGCCGGGGGC ATGGCCATGC TGACGCTGAG CGACACCGAG 
AACAACCTAC ACTTCATCCT CATGGCCCGT GGGCTGCTGG AGCCCGGTGC TGAGGGTGAG 
GGTGGGCATG GGCGCCTGCA AGGCATGGCA TCACCAGTGG GGGGCTCAAG GGAGTCCCCC 
TGGGTACCGC TGCGGGTCCG CATCCTGCAC CAGGGCCAGA CGCTGCGCGA GGCCCATGCC 
AACATCACTA TGGAGGACCC CGACTTCGCG GAGGTACTGA GCGACCTGTC TGCCCATGAG 
CTGCAGTGGT TGGCACAGGG GCAGCTCCGC ATCGTGGCTG ACACCCAGGG CCGGCACCCG 
CGCCAGCTGG AGGGCACCAT TACCGCCCGC CGCAGCTGTG ACACCATCCA AAGCGTGCTG 
TGCGGCGCAG ATGCATTGCA GCCCACCAAG ACGGGGGCGG TGGGCTCGGC CAAGCTGGCG 
CTGCATGAGA ACGGCACGCT GGAGTACCAG GTGCGGGTGG TGGGCACGGC CAGCGAGGTG 
GTGGGGGTCA CACTGGAGAC CAAACCGCGG CGGAAGAACA GGAGGAACGT CCTCTTCGAC 
ATGACACCCA GCTATAGGGC TGGGATGGCT CAAGGAGTGT GGCACAGCCC CAGCGCCCGC 
GATGTGCACA TGCTGCTGCA GAATGAGCTC TTCCTCAACG TGGCCACCAA GGACTGGGCA 
GAGGGAGAGC TGCGGGGCCA GGTCATCTCC CTGCCCTACA GTGGGCTCCT GGCACGCTAC 
ACAGAGATGC CGGTGCCGCT GGCAGGGCAG CTGGTATCCC CCCCAGTGAG CAGCGGGGCC 
GGGGGCCACG CGTGGCTGTC ACTGGATGAG CACTGCCACC TGCACTACGA GATAGTGGTG 
GCGGGTCTGG GCCGCCCGGC CGATGGCACA GTCAGTGCCC AGCTGCACGG CGTGGCCGAG 
CTGGGCGAGA TGGGCACTCG GCCGCACCTG CACAAGCGGA TGCTCAAGGG GTTCTATGGC 
ACTGAGGCTC AGGGGGTGGT GAAGGACCTG GATGCTGAGC TGCTGCAGCA CCTGGCACAG 
GGCACCGCAT TCCTGCAAGT CAGCACCAAA GCACACCCGC GTGGGGAGAT GCGGGGATGG 
GTGCACATCC CCAACCGCTG TCAGCCAGGA GGGGCCCGCC TGTCCACCGG GGAGGCTGAG 
CTCTCAGAGG GCCCTAAGGG CAGGAATGTG GAACAGCTGA AAAAGGACCC CAACTCCTGC 
TTCTTCGAGG GGCAGCACCA TGCACACGGC ACCCGCTGGG CGCCTGACTA CGACAAGAAG 
TGCTCCATCT GCAGCTGCCA GAAGCGCACG GTCATCTGCG ATCCCATCCT GTGCCAGCCC 
CTCAACTGTA CCCGCCAGGT GCACCCTGAG GAGCTGTGCT GCCCCATCTG TGAAGAGAAG 
AAGATGGAGC AGGAGGAGCT GAAGCTGGAA CGGGCACGGG ACAGCAGCGA GGGCTGCTAC 
TTTGATGGGG ACAAGACATG GCGAGGCTCT GGCACCCGCT GGCACCCTGT CGTGCCGCCG 
TTTGGCCTCA TCAAATGTGC CATCTGCACC TGCAAGGGCA CCACGGGTGA AGTGCACTGC 
GAGAAGGTGC AGTGTCCGCG CCTCACCTGT GCCAACCCTG TGCGCGTCAG CCCCTCCGAC 
TGCTGCAAGC AGTGCCCAGC CCCTGAGAAG AGCATCCCTG AGCTGACTGA TGGCATGCAG 
GCGGACGGAC CCCGTGCGTG CCGCTTTGGG CGCCGCTGGT ACCTCAACAA CGAGAGCTGG 
CACCCCTCAG TGCCACCCTT TGGGGAGATG AAGTGCATCC TGTGCTGGTG TGTGTCAGGA 
GAGACCCACT GCCAGCGCCA GGAGTGCCCG CCAGATGCCT GTGCCAGCCC CACCAGGAGG 
GACAACCCCT GCTGTGCCAA GTGTCGCGCT CCGGATGCAC CCTCAGAAAA GATGTACGAA 
GCCACAGCAG AGGCATGGAG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRD ( XM_015291372.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146858.1 Gallus gallus chordin (CHRD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa39870
Clone ID Related Accession (Same CDS sequence)	XM_015291372.2 , XM_015291372.1
Accession Version	XM_015291372.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	chordin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291372.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGT TTCCCCCATG CCAAGGCCGC GGCTCTCCCA GCTTTCCTTC CTTCCCGGGA 
AAGGGGAGGG AGCTTCCCCT GTTGCTTCCC CACATCCCCG CAGCCGAGCC AGGAGACTGT 
GACGAGCTCC GGGTGCGCGG AGCCAGCGGC GGATTCCTGG CACCGGCGGG GCGGCCGACA 
GCCATCGCCC GCCTCCTCCT CCCCGCTCTG CCCCGCTCGC TCCCCGCCGT TCCCCCCGGG 
CTGCGCCACG GTGGTGGGCA CCGGGCGGTG ACGCTGGCAC GGCCACGCAG CCCCCAGCCC 
TCCCCTGCCA CCGGATCCCA GGCCAACAGG GACAACGGGA GAGAGGAGCA GGGACAGGCC 
TTCCCATGGT GTCTGCTCCC TGCCCACCAC CGCTGCTGCC CTCATCAGCC CGGGCACATG 
TGGGTGAAGA GCAGGATCCG TGCTGGGTGC TGTTCCCATA CCCTCGGCCG CGGCACCTCC 
CTGGGACCCC AGGTCTTGTC CCCACGCTCG TGGGAACGGT CCTGTGGTCC TGCTCTGGGA 
TTGCCATCTG CACCAGGATT TGGGGATGCC AAAGGCACAT GTTGTGGGGG CTCGCAGCTC 
CCGCTTGCCC ATGCCGCCGC CTATAAAGCG GGGCCGGGGC CGGCGGCCGG GCAGAGGCGG 
TGGCAGCGGA GGCAGCAGGC AGCGCCCGGC CCCCCCTCCC CGCCCCCGGC CGCCCGGTTC 
CCCCGGTTCC CCGGCACCAT GCGCACCGCC CTGCTGCTGC TCGCCCTGCT CGCCCTGCCC 
GTCCGCACCG CCCGCCCCAA GCTCGCCCTG CCCATCCGGC CCGACAGCGA GCCGCTGCCC 
CCCGGGGGGG CGGCAGGTTG TGCCTTCGGG GGACGTTTCT ACGCGCTGGA GGAGACGTGG 
CACCCCGACC TGGGGGAGCC CTTTGGGGTG ATGCGCTGCG TGATCTGCCA CTGCGAGACG 
CAGAGGAATC GCCGAGGGAA ACCAGTGGGG AAAGTGAACT GCAAGAACAT GAAGCAGGAT 
TGCCCCGTTC CCACCTGCCC CCGTGCCACG CTGTTGCCCG GGCACTGCTG CCATACCTGT 
CCCAAAGCCT TGCCAGGAGC CCCGGAGAAG AGCTACAAGC CACCTTTTGA CACCTTTGAG 
TACTTCCAGG ACAAGGAGGA CGAGCTGGAC AAGCCGTACA ATGACCGCTC CTACCTCAGC 
TCTGAGGGTT CGGCACGTGA CGATGCCCGC ACAGAATTCG TGGCCCTGCT GACGAGCGGC 
CCCGAGCCGT GGCACCCCAC ATCCAGCGCG GTGGCCAAGG CTCGCTTCAC CCTGCTGCGC 
TCCTACCTGC TCTTCTCCAT CAGCTACGAG CGGCTGGGCC GGCCGAGCCG TGTGCGTTTC 
AGTGACCCCG AGGGCACCGT GCTGTTCGAG CACCCAGTGC AGAAGAGCGC AGCCCCTGAG 
GACGGCATGC TCTGTGGGAT GTGGAGGACA GTGTCCAAAG CCAACATACA GCTGCTGCGT 
GCGGAGCAGC TCCGTGTGTC CCTCATCACC CGTGCGCAGC CCTCCGGAGA GGTGCATGGG 
CACATCCTGA AGCACCGGGC GCTCTTTGCA GAGACATTTG GTGCCATCCT GACCTCATCA 
GACCCCGCGC ACCTGGGGGC CGGGGGCATG GCCATGCTGA CGCTGAGCGA CACCGAGAAC 
AACCTACACT TCATCCTCAT GGCCCGTGGG CTGCTGGAGC CCGGTGCTGA GGGTGAGGGT 
GGGCATGGGC GCCTGCAAGG CATGGCATCA CCAGTGGGGG GCTCAAGGGA GTCCCCCTGG 
GTACCGCTGC GGGTCCGCAT CCTGCACCAG GGCCAGACGC TGCGCGAGGC CCATGCCAAC 
ATCACTATGG AGGACCCCGA CTTCGCGGAG GTACTGAGCG ACCTGTCTGC CCATGAGCTG 
CAGTGGTTGG CACAGGGGCA GCTCCGCATC GTGGCTGACA CCCAGGGCCG GCACCCGCGC 
CAGCTGGAGG GCACCATTAC CGCCCGCCGC AGCTGTGACA CCATCCAAAG CGTGCTGTGC 
GGCGCAGATG CATTGCAGCC CACCAAGACG GGGGCGGTGG GCTCGGCCAA GCTGGCGCTG 
CATGAGAACG GCACGCTGGA GTACCAGGTG CGGGTGGTGG GCACGGCCAG CGAGGTGGTG 
GGGGTCACAC TGGAGACCAA ACCGCGGCGG AAGAACAGGA GGAACGTCCT CTTCGACATG 
ACACCCAGCT ATAGGGCTGG GATGGCTCAA GGAGTGTGGC ACAGCCCCAG CGCCCGCGAT 
GTGCACATGC TGCTGCAGAA TGAGCTCTTC CTCAACGTGG CCACCAAGGA CTGGGCAGAG 
GGAGAGCTGC GGGGCCAGGT CATCTCCCTG CCCTACAGTG GGCTCCTGGC ACGCTACACA 
GAGATGCCGG TGCCGCTGGC AGGGCAGCTG GTATCCCCCC CAGTGAGCAG CGGGGCCGGG 
GGCCACGCGT GGCTGTCACT GGATGAGCAC TGCCACCTGC ACTACGAGAT AGTGGTGGCG 
GGTCTGGGCC GCCCGGCCGA TGGCACAGTC AGTGCCCAGC TGCACGGCGT GGCCGAGCTG 
GGCGAGATGG GCACTCGGCC GCACCTGCAC AAGCGGATGC TCAAGGGGTT CTATGGCACT 
GAGGCTCAGG GGGTGGTGAA GGACCTGGAT GCTGAGCTGC TGCAGCACCT GGCACAGGGC 
ACCGCATTCC TGCAAGTCAG CACCAAAGCA CACCCGCGTG GGGAGATGCG GGGATGGGTG 
CACATCCCCA ACCGCTGTCA GCCAGGAGGG GCCCGCCTGT CCACCGGGGA GGCTGAGCTC 
TCAGAGGGCC CTAAGGGCAG GAATGTGGAA CAGCTGAAAA AGGACCCCAA CTCCTGCTTC 
TTCGAGGGGC AGCACCATGC ACACGGCACC CGCTGGGCGC CTGACTACGA CAAGAAGTGC 
TCCATCTGCA GCTGCCAGAA GCGCACGGTC ATCTGCGATC CCATCCTGTG CCAGCCCCTC 
AACTGTACCC GCCAGGTGCA CCCTGAGGAG CTGTGCTGCC CCATCTGTGA AGAGAAGAAG 
ATGGAGCAGG AGGAGCTGAA GCTGGAACGG GCACGGGACA GCAGCGAGGG CTGCTACTTT 
GATGGGGACA AGACATGGCG AGGCTCTGGC ACCCGCTGGC ACCCTGTCGT GCCGCCGTTT 
GGCCTCATCA AATGTGCCAT CTGCACCTGC AAGGGCACCA CGGGTGAAGT GCACTGCGAG 
AAGGTGCAGT GTCCGCGCCT CACCTGTGCC AACCCTGTGC GCGTCAGCCC CTCCGACTGC 
TGCAAGCAGT GCCCAGCCCC TGAGAAGAGC ATCCCTGAGC TGACTGATGG CATGCAGGCG 
GACGGACCCC GTGCGTGCCG CTTTGGGCGC CGCTGGTACC TCAACAACGA GAGCTGGCAC 
CCCTCAGTGC CACCCTTTGG GGAGATGAAG TGCATCCTGT GCTGGTGTGT GTCAGGAGAG 
ACCCACTGCC AGCGCCAGGA GTGCCCGCCA GATGCCTGTG CCAGCCCCAC CAGGAGGGAC 
AACCCCTGCT GTGCCAAGTG TCGCGCTCCG GATGCACCCT CAGAAAAGAT GTACGAAGCC 
ACAGCAGAGG CATGGAGCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146858.1
CDS	35..3658
Translation	MPQFPPCQGRGSPSFPSFPGKGRELPLLLPHIPAAEPGDCDELRVRGASGGFLAPAGRPTAIARLLLPALPRSLPAVPPGLRHGGGHRAVTLARPRSPQPSPATGSQANRDNGREEQGQAFPWCLLPAHHRCCPHQPGHMWVKSRIRAGCCSHTLGRGTSLGPQVLSPRSWERSCGPALGLPSAPGFGDAKGTCCGGSQLPLAHAAAYKAGPGPAAGQRRWQRRQQAAPGPPSPPPAARFPRFPGTMRTALLLLALLALPVRTARPKLALPIRPDSEPLPPGGAAGCAFGGRFYALEETWHPDLGEPFGVMRCVICHCETQRNRRGKPVGKVNCKNMKQDCPVPTCPRATLLPGHCCHTCPKALPGAPEKSYKPPFDTFEYFQDKEDELDKPYNDRSYLSSEGSARDDARTEFVALLTSGPEPWHPTSSAVAKARFTLLRSYLLFSISYERLGRPSRVRFSDPEGTVLFEHPVQKSAAPEDGMLCGMWRTVSKANIQLLRAEQLRVSLITRAQPSGEVHGHILKHRALFAETFGAILTSSDPAHLGAGGMAMLTLSDTENNLHFILMARGLLEPGAEGEGGHGRLQGMASPVGGSRESPWVPLRVRILHQGQTLREAHANITMEDPDFAEVLSDLSAHELQWLAQGQLRIVADTQGRHPRQLEGTITARRSCDTIQSVLCGADALQPTKTGAVGSAKLALHENGTLEYQVRVVGTASEVVGVTLETKPRRKNRRNVLFDMTPSYRAGMAQGVWHSPSARDVHMLLQNELFLNVATKDWAEGELRGQVISLPYSGLLARYTEMPVPLAGQLVSPPVSSGAGGHAWLSLDEHCHLHYEIVVAGLGRPADGTVSAQLHGVAELGEMGTRPHLHKRMLKGFYGTEAQGVVKDLDAELLQHLAQGTAFLQVSTKAHPRGEMRGWVHIPNRCQPGGARLSTGEAELSEGPKGRNVEQLKKDPNSCFFEGQHHAHGTRWAPDYDKKCSICSCQKRTVICDPILCQPLNCTRQVHPEELCCPICEEKKMEQEELKLERARDSSEGCYFDGDKTWRGSGTRWHPVVPPFGLIKCAICTCKGTTGEVHCEKVQCPRLTCANPVRVSPSDCCKQCPAPEKSIPELTDGMQADGPRACRFGRRWYLNNESWHPSVPPFGEMKCILCWCVSGETHCQRQECPPDACASPTRRDNPCCAKCRAPDAPSEKMYEATAEAWSR

Target ORF information:

RefSeq Version	XM_015291372.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chordin (CHRD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291372.2

ORF Insert Sequence:

ATGCCTCAGT TTCCCCCATG CCAAGGCCGC GGCTCTCCCA GCTTTCCTTC CTTCCCGGGA 
AAGGGGAGGG AGCTTCCCCT GTTGCTTCCC CACATCCCCG CAGCCGAGCC AGGAGACTGT 
GACGAGCTCC GGGTGCGCGG AGCCAGCGGC GGATTCCTGG CACCGGCGGG GCGGCCGACA 
GCCATCGCCC GCCTCCTCCT CCCCGCTCTG CCCCGCTCGC TCCCCGCCGT TCCCCCCGGG 
CTGCGCCACG GTGGTGGGCA CCGGGCGGTG ACGCTGGCAC GGCCACGCAG CCCCCAGCCC 
TCCCCTGCCA CCGGATCCCA GGCCAACAGG GACAACGGGA GAGAGGAGCA GGGACAGGCC 
TTCCCATGGT GTCTGCTCCC TGCCCACCAC CGCTGCTGCC CTCATCAGCC CGGGCACATG 
TGGGTGAAGA GCAGGATCCG TGCTGGGTGC TGTTCCCATA CCCTCGGCCG CGGCACCTCC 
CTGGGACCCC AGGTCTTGTC CCCACGCTCG TGGGAACGGT CCTGTGGTCC TGCTCTGGGA 
TTGCCATCTG CACCAGGATT TGGGGATGCC AAAGGCACAT GTTGTGGGGG CTCGCAGCTC 
CCGCTTGCCC ATGCCGCCGC CTATAAAGCG GGGCCGGGGC CGGCGGCCGG GCAGAGGCGG 
TGGCAGCGGA GGCAGCAGGC AGCGCCCGGC CCCCCCTCCC CGCCCCCGGC CGCCCGGTTC 
CCCCGGTTCC CCGGCACCAT GCGCACCGCC CTGCTGCTGC TCGCCCTGCT CGCCCTGCCC 
GTCCGCACCG CCCGCCCCAA GCTCGCCCTG CCCATCCGGC CCGACAGCGA GCCGCTGCCC 
CCCGGGGGGG CGGCAGGTTG TGCCTTCGGG GGACGTTTCT ACGCGCTGGA GGAGACGTGG 
CACCCCGACC TGGGGGAGCC CTTTGGGGTG ATGCGCTGCG TGATCTGCCA CTGCGAGACG 
CAGAGGAATC GCCGAGGGAA ACCAGTGGGG AAAGTGAACT GCAAGAACAT GAAGCAGGAT 
TGCCCCGTTC CCACCTGCCC CCGTGCCACG CTGTTGCCCG GGCACTGCTG CCATACCTGT 
CCCAAAGCCT TGCCAGGAGC CCCGGAGAAG AGCTACAAGC CACCTTTTGA CACCTTTGAG 
TACTTCCAGG ACAAGGAGGA CGAGCTGGAC AAGCCGTACA ATGACCGCTC CTACCTCAGC 
TCTGAGGGTT CGGCACGTGA CGATGCCCGC ACAGAATTCG TGGCCCTGCT GACGAGCGGC 
CCCGAGCCGT GGCACCCCAC ATCCAGCGCG GTGGCCAAGG CTCGCTTCAC CCTGCTGCGC 
TCCTACCTGC TCTTCTCCAT CAGCTACGAG CGGCTGGGCC GGCCGAGCCG TGTGCGTTTC 
AGTGACCCCG AGGGCACCGT GCTGTTCGAG CACCCAGTGC AGAAGAGCGC AGCCCCTGAG 
GACGGCATGC TCTGTGGGAT GTGGAGGACA GTGTCCAAAG CCAACATACA GCTGCTGCGT 
GCGGAGCAGC TCCGTGTGTC CCTCATCACC CGTGCGCAGC CCTCCGGAGA GGTGCATGGG 
CACATCCTGA AGCACCGGGC GCTCTTTGCA GAGACATTTG GTGCCATCCT GACCTCATCA 
GACCCCGCGC ACCTGGGGGC CGGGGGCATG GCCATGCTGA CGCTGAGCGA CACCGAGAAC 
AACCTACACT TCATCCTCAT GGCCCGTGGG CTGCTGGAGC CCGGTGCTGA GGGTGAGGGT 
GGGCATGGGC GCCTGCAAGG CATGGCATCA CCAGTGGGGG GCTCAAGGGA GTCCCCCTGG 
GTACCGCTGC GGGTCCGCAT CCTGCACCAG GGCCAGACGC TGCGCGAGGC CCATGCCAAC 
ATCACTATGG AGGACCCCGA CTTCGCGGAG GTACTGAGCG ACCTGTCTGC CCATGAGCTG 
CAGTGGTTGG CACAGGGGCA GCTCCGCATC GTGGCTGACA CCCAGGGCCG GCACCCGCGC 
CAGCTGGAGG GCACCATTAC CGCCCGCCGC AGCTGTGACA CCATCCAAAG CGTGCTGTGC 
GGCGCAGATG CATTGCAGCC CACCAAGACG GGGGCGGTGG GCTCGGCCAA GCTGGCGCTG 
CATGAGAACG GCACGCTGGA GTACCAGGTG CGGGTGGTGG GCACGGCCAG CGAGGTGGTG 
GGGGTCACAC TGGAGACCAA ACCGCGGCGG AAGAACAGGA GGAACGTCCT CTTCGACATG 
ACACCCAGCT ATAGGGCTGG GATGGCTCAA GGAGTGTGGC ACAGCCCCAG CGCCCGCGAT 
GTGCACATGC TGCTGCAGAA TGAGCTCTTC CTCAACGTGG CCACCAAGGA CTGGGCAGAG 
GGAGAGCTGC GGGGCCAGGT CATCTCCCTG CCCTACAGTG GGCTCCTGGC ACGCTACACA 
GAGATGCCGG TGCCGCTGGC AGGGCAGCTG GTATCCCCCC CAGTGAGCAG CGGGGCCGGG 
GGCCACGCGT GGCTGTCACT GGATGAGCAC TGCCACCTGC ACTACGAGAT AGTGGTGGCG 
GGTCTGGGCC GCCCGGCCGA TGGCACAGTC AGTGCCCAGC TGCACGGCGT GGCCGAGCTG 
GGCGAGATGG GCACTCGGCC GCACCTGCAC AAGCGGATGC TCAAGGGGTT CTATGGCACT 
GAGGCTCAGG GGGTGGTGAA GGACCTGGAT GCTGAGCTGC TGCAGCACCT GGCACAGGGC 
ACCGCATTCC TGCAAGTCAG CACCAAAGCA CACCCGCGTG GGGAGATGCG GGGATGGGTG 
CACATCCCCA ACCGCTGTCA GCCAGGAGGG GCCCGCCTGT CCACCGGGGA GGCTGAGCTC 
TCAGAGGGCC CTAAGGGCAG GAATGTGGAA CAGCTGAAAA AGGACCCCAA CTCCTGCTTC 
TTCGAGGGGC AGCACCATGC ACACGGCACC CGCTGGGCGC CTGACTACGA CAAGAAGTGC 
TCCATCTGCA GCTGCCAGAA GCGCACGGTC ATCTGCGATC CCATCCTGTG CCAGCCCCTC 
AACTGTACCC GCCAGGTGCA CCCTGAGGAG CTGTGCTGCC CCATCTGTGA AGAGAAGAAG 
ATGGAGCAGG AGGAGCTGAA GCTGGAACGG GCACGGGACA GCAGCGAGGG CTGCTACTTT 
GATGGGGACA AGACATGGCG AGGCTCTGGC ACCCGCTGGC ACCCTGTCGT GCCGCCGTTT 
GGCCTCATCA AATGTGCCAT CTGCACCTGC AAGGGCACCA CGGGTGAAGT GCACTGCGAG 
AAGGTGCAGT GTCCGCGCCT CACCTGTGCC AACCCTGTGC GCGTCAGCCC CTCCGACTGC 
TGCAAGCAGT GCCCAGCCCC TGAGAAGAGC ATCCCTGAGC TGACTGATGG CATGCAGGCG 
GACGGACCCC GTGCGTGCCG CTTTGGGCGC CGCTGGTACC TCAACAACGA GAGCTGGCAC 
CCCTCAGTGC CACCCTTTGG GGAGATGAAG TGCATCCTGT GCTGGTGTGT GTCAGGAGAG 
ACCCACTGCC AGCGCCAGGA GTGCCCGCCA GATGCCTGTG CCAGCCCCAC CAGGAGGGAC 
AACCCCTGCT GTGCCAAGTG TCGCGCTCCG GATGCACCCT CAGAAAAGAT GTACGAAGCC 
ACAGCAGAGG CATGGAGCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRD ( XM_015291372.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146858.1 Gallus gallus chordin (CHRD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa39870
Clone ID Related Accession (Same CDS sequence)	XM_015291372.2 , XM_015291372.1
Accession Version	XM_015291372.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	chordin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGT TTCCCCCATG CCAAGGCCGC GGCTCTCCCA GCTTTCCTTC CTTCCCGGGA 
AAGGGGAGGG AGCTTCCCCT GTTGCTTCCC CACATCCCCG CAGCCGAGCC AGGAGACTGT 
GACGAGCTCC GGGTGCGCGG AGCCAGCGGC GGATTCCTGG CACCGGCGGG GCGGCCGACA 
GCCATCGCCC GCCTCCTCCT CCCCGCTCTG CCCCGCTCGC TCCCCGCCGT TCCCCCCGGG 
CTGCGCCACG GTGGTGGGCA CCGGGCGGTG ACGCTGGCAC GGCCACGCAG CCCCCAGCCC 
TCCCCTGCCA CCGGATCCCA GGCCAACAGG GACAACGGGA GAGAGGAGCA GGGACAGGCC 
TTCCCATGGT GTCTGCTCCC TGCCCACCAC CGCTGCTGCC CTCATCAGCC CGGGCACATG 
TGGGTGAAGA GCAGGATCCG TGCTGGGTGC TGTTCCCATA CCCTCGGCCG CGGCACCTCC 
CTGGGACCCC AGGTCTTGTC CCCACGCTCG TGGGAACGGT CCTGTGGTCC TGCTCTGGGA 
TTGCCATCTG CACCAGGATT TGGGGATGCC AAAGGCACAT GTTGTGGGGG CTCGCAGCTC 
CCGCTTGCCC ATGCCGCCGC CTATAAAGCG GGGCCGGGGC CGGCGGCCGG GCAGAGGCGG 
TGGCAGCGGA GGCAGCAGGC AGCGCCCGGC CCCCCCTCCC CGCCCCCGGC CGCCCGGTTC 
CCCCGGTTCC CCGGCACCAT GCGCACCGCC CTGCTGCTGC TCGCCCTGCT CGCCCTGCCC 
GTCCGCACCG CCCGCCCCAA GCTCGCCCTG CCCATCCGGC CCGACAGCGA GCCGCTGCCC 
CCCGGGGGGG CGGCAGGTTG TGCCTTCGGG GGACGTTTCT ACGCGCTGGA GGAGACGTGG 
CACCCCGACC TGGGGGAGCC CTTTGGGGTG ATGCGCTGCG TGATCTGCCA CTGCGAGACG 
CAGAGGAATC GCCGAGGGAA ACCAGTGGGG AAAGTGAACT GCAAGAACAT GAAGCAGGAT 
TGCCCCGTTC CCACCTGCCC CCGTGCCACG CTGTTGCCCG GGCACTGCTG CCATACCTGT 
CCCAAAGCCT TGCCAGGAGC CCCGGAGAAG AGCTACAAGC CACCTTTTGA CACCTTTGAG 
TACTTCCAGG ACAAGGAGGA CGAGCTGGAC AAGCCGTACA ATGACCGCTC CTACCTCAGC 
TCTGAGGGTT CGGCACGTGA CGATGCCCGC ACAGAATTCG TGGCCCTGCT GACGAGCGGC 
CCCGAGCCGT GGCACCCCAC ATCCAGCGCG GTGGCCAAGG CTCGCTTCAC CCTGCTGCGC 
TCCTACCTGC TCTTCTCCAT CAGCTACGAG CGGCTGGGCC GGCCGAGCCG TGTGCGTTTC 
AGTGACCCCG AGGGCACCGT GCTGTTCGAG CACCCAGTGC AGAAGAGCGC AGCCCCTGAG 
GACGGCATGC TCTGTGGGAT GTGGAGGACA GTGTCCAAAG CCAACATACA GCTGCTGCGT 
GCGGAGCAGC TCCGTGTGTC CCTCATCACC CGTGCGCAGC CCTCCGGAGA GGTGCATGGG 
CACATCCTGA AGCACCGGGC GCTCTTTGCA GAGACATTTG GTGCCATCCT GACCTCATCA 
GACCCCGCGC ACCTGGGGGC CGGGGGCATG GCCATGCTGA CGCTGAGCGA CACCGAGAAC 
AACCTACACT TCATCCTCAT GGCCCGTGGG CTGCTGGAGC CCGGTGCTGA GGGTGAGGGT 
GGGCATGGGC GCCTGCAAGG CATGGCATCA CCAGTGGGGG GCTCAAGGGA GTCCCCCTGG 
GTACCGCTGC GGGTCCGCAT CCTGCACCAG GGCCAGACGC TGCGCGAGGC CCATGCCAAC 
ATCACTATGG AGGACCCCGA CTTCGCGGAG GTACTGAGCG ACCTGTCTGC CCATGAGCTG 
CAGTGGTTGG CACAGGGGCA GCTCCGCATC GTGGCTGACA CCCAGGGCCG GCACCCGCGC 
CAGCTGGAGG GCACCATTAC CGCCCGCCGC AGCTGTGACA CCATCCAAAG CGTGCTGTGC 
GGCGCAGATG CATTGCAGCC CACCAAGACG GGGGCGGTGG GCTCGGCCAA GCTGGCGCTG 
CATGAGAACG GCACGCTGGA GTACCAGGTG CGGGTGGTGG GCACGGCCAG CGAGGTGGTG 
GGGGTCACAC TGGAGACCAA ACCGCGGCGG AAGAACAGGA GGAACGTCCT CTTCGACATG 
ACACCCAGCT ATAGGGCTGG GATGGCTCAA GGAGTGTGGC ACAGCCCCAG CGCCCGCGAT 
GTGCACATGC TGCTGCAGAA TGAGCTCTTC CTCAACGTGG CCACCAAGGA CTGGGCAGAG 
GGAGAGCTGC GGGGCCAGGT CATCTCCCTG CCCTACAGTG GGCTCCTGGC ACGCTACACA 
GAGATGCCGG TGCCGCTGGC AGGGCAGCTG GTATCCCCCC CAGTGAGCAG CGGGGCCGGG 
GGCCACGCGT GGCTGTCACT GGATGAGCAC TGCCACCTGC ACTACGAGAT AGTGGTGGCG 
GGTCTGGGCC GCCCGGCCGA TGGCACAGTC AGTGCCCAGC TGCACGGCGT GGCCGAGCTG 
GGCGAGATGG GCACTCGGCC GCACCTGCAC AAGCGGATGC TCAAGGGGTT CTATGGCACT 
GAGGCTCAGG GGGTGGTGAA GGACCTGGAT GCTGAGCTGC TGCAGCACCT GGCACAGGGC 
ACCGCATTCC TGCAAGTCAG CACCAAAGCA CACCCGCGTG GGGAGATGCG GGGATGGGTG 
CACATCCCCA ACCGCTGTCA GCCAGGAGGG GCCCGCCTGT CCACCGGGGA GGCTGAGCTC 
TCAGAGGGCC CTAAGGGCAG GAATGTGGAA CAGCTGAAAA AGGACCCCAA CTCCTGCTTC 
TTCGAGGGGC AGCACCATGC ACACGGCACC CGCTGGGCGC CTGACTACGA CAAGAAGTGC 
TCCATCTGCA GCTGCCAGAA GCGCACGGTC ATCTGCGATC CCATCCTGTG CCAGCCCCTC 
AACTGTACCC GCCAGGTGCA CCCTGAGGAG CTGTGCTGCC CCATCTGTGA AGAGAAGAAG 
ATGGAGCAGG AGGAGCTGAA GCTGGAACGG GCACGGGACA GCAGCGAGGG CTGCTACTTT 
GATGGGGACA AGACATGGCG AGGCTCTGGC ACCCGCTGGC ACCCTGTCGT GCCGCCGTTT 
GGCCTCATCA AATGTGCCAT CTGCACCTGC AAGGGCACCA CGGGTGAAGT GCACTGCGAG 
AAGGTGCAGT GTCCGCGCCT CACCTGTGCC AACCCTGTGC GCGTCAGCCC CTCCGACTGC 
TGCAAGCAGT GCCCAGCCCC TGAGAAGAGC ATCCCTGAGC TGACTGATGG CATGCAGGCG 
GACGGACCCC GTGCGTGCCG CTTTGGGCGC CGCTGGTACC TCAACAACGA GAGCTGGCAC 
CCCTCAGTGC CACCCTTTGG GGAGATGAAG TGCATCCTGT GCTGGTGTGT GTCAGGAGAG 
ACCCACTGCC AGCGCCAGGA GTGCCCGCCA GATGCCTGTG CCAGCCCCAC CAGGAGGGAC 
AACCCCTGCT GTGCCAAGTG TCGCGCTCCG GATGCACCCT CAGAAAAGAT GTACGAAGCC 
ACAGCAGAGG CATGGAGCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146858.1
CDS	19..3642
Translation	MPQFPPCQGRGSPSFPSFPGKGRELPLLLPHIPAAEPGDCDELRVRGASGGFLAPAGRPTAIARLLLPALPRSLPAVPPGLRHGGGHRAVTLARPRSPQPSPATGSQANRDNGREEQGQAFPWCLLPAHHRCCPHQPGHMWVKSRIRAGCCSHTLGRGTSLGPQVLSPRSWERSCGPALGLPSAPGFGDAKGTCCGGSQLPLAHAAAYKAGPGPAAGQRRWQRRQQAAPGPPSPPPAARFPRFPGTMRTALLLLALLALPVRTARPKLALPIRPDSEPLPPGGAAGCAFGGRFYALEETWHPDLGEPFGVMRCVICHCETQRNRRGKPVGKVNCKNMKQDCPVPTCPRATLLPGHCCHTCPKALPGAPEKSYKPPFDTFEYFQDKEDELDKPYNDRSYLSSEGSARDDARTEFVALLTSGPEPWHPTSSAVAKARFTLLRSYLLFSISYERLGRPSRVRFSDPEGTVLFEHPVQKSAAPEDGMLCGMWRTVSKANIQLLRAEQLRVSLITRAQPSGEVHGHILKHRALFAETFGAILTSSDPAHLGAGGMAMLTLSDTENNLHFILMARGLLEPGAEGEGGHGRLQGMASPVGGSRESPWVPLRVRILHQGQTLREAHANITMEDPDFAEVLSDLSAHELQWLAQGQLRIVADTQGRHPRQLEGTITARRSCDTIQSVLCGADALQPTKTGAVGSAKLALHENGTLEYQVRVVGTASEVVGVTLETKPRRKNRRNVLFDMTPSYRAGMAQGVWHSPSARDVHMLLQNELFLNVATKDWAEGELRGQVISLPYSGLLARYTEMPVPLAGQLVSPPVSSGAGGHAWLSLDEHCHLHYEIVVAGLGRPADGTVSAQLHGVAELGEMGTRPHLHKRMLKGFYGTEAQGVVKDLDAELLQHLAQGTAFLQVSTKAHPRGEMRGWVHIPNRCQPGGARLSTGEAELSEGPKGRNVEQLKKDPNSCFFEGQHHAHGTRWAPDYDKKCSICSCQKRTVICDPILCQPLNCTRQVHPEELCCPICEEKKMEQEELKLERARDSSEGCYFDGDKTWRGSGTRWHPVVPPFGLIKCAICTCKGTTGEVHCEKVQCPRLTCANPVRVSPSDCCKQCPAPEKSIPELTDGMQADGPRACRFGRRWYLNNESWHPSVPPFGEMKCILCWCVSGETHCQRQECPPDACASPTRRDNPCCAKCRAPDAPSEKMYEATAEAWSR

Target ORF information:

RefSeq Version	XM_015291372.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chordin (CHRD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291372.1

ORF Insert Sequence:

ATGCCTCAGT TTCCCCCATG CCAAGGCCGC GGCTCTCCCA GCTTTCCTTC CTTCCCGGGA 
AAGGGGAGGG AGCTTCCCCT GTTGCTTCCC CACATCCCCG CAGCCGAGCC AGGAGACTGT 
GACGAGCTCC GGGTGCGCGG AGCCAGCGGC GGATTCCTGG CACCGGCGGG GCGGCCGACA 
GCCATCGCCC GCCTCCTCCT CCCCGCTCTG CCCCGCTCGC TCCCCGCCGT TCCCCCCGGG 
CTGCGCCACG GTGGTGGGCA CCGGGCGGTG ACGCTGGCAC GGCCACGCAG CCCCCAGCCC 
TCCCCTGCCA CCGGATCCCA GGCCAACAGG GACAACGGGA GAGAGGAGCA GGGACAGGCC 
TTCCCATGGT GTCTGCTCCC TGCCCACCAC CGCTGCTGCC CTCATCAGCC CGGGCACATG 
TGGGTGAAGA GCAGGATCCG TGCTGGGTGC TGTTCCCATA CCCTCGGCCG CGGCACCTCC 
CTGGGACCCC AGGTCTTGTC CCCACGCTCG TGGGAACGGT CCTGTGGTCC TGCTCTGGGA 
TTGCCATCTG CACCAGGATT TGGGGATGCC AAAGGCACAT GTTGTGGGGG CTCGCAGCTC 
CCGCTTGCCC ATGCCGCCGC CTATAAAGCG GGGCCGGGGC CGGCGGCCGG GCAGAGGCGG 
TGGCAGCGGA GGCAGCAGGC AGCGCCCGGC CCCCCCTCCC CGCCCCCGGC CGCCCGGTTC 
CCCCGGTTCC CCGGCACCAT GCGCACCGCC CTGCTGCTGC TCGCCCTGCT CGCCCTGCCC 
GTCCGCACCG CCCGCCCCAA GCTCGCCCTG CCCATCCGGC CCGACAGCGA GCCGCTGCCC 
CCCGGGGGGG CGGCAGGTTG TGCCTTCGGG GGACGTTTCT ACGCGCTGGA GGAGACGTGG 
CACCCCGACC TGGGGGAGCC CTTTGGGGTG ATGCGCTGCG TGATCTGCCA CTGCGAGACG 
CAGAGGAATC GCCGAGGGAA ACCAGTGGGG AAAGTGAACT GCAAGAACAT GAAGCAGGAT 
TGCCCCGTTC CCACCTGCCC CCGTGCCACG CTGTTGCCCG GGCACTGCTG CCATACCTGT 
CCCAAAGCCT TGCCAGGAGC CCCGGAGAAG AGCTACAAGC CACCTTTTGA CACCTTTGAG 
TACTTCCAGG ACAAGGAGGA CGAGCTGGAC AAGCCGTACA ATGACCGCTC CTACCTCAGC 
TCTGAGGGTT CGGCACGTGA CGATGCCCGC ACAGAATTCG TGGCCCTGCT GACGAGCGGC 
CCCGAGCCGT GGCACCCCAC ATCCAGCGCG GTGGCCAAGG CTCGCTTCAC CCTGCTGCGC 
TCCTACCTGC TCTTCTCCAT CAGCTACGAG CGGCTGGGCC GGCCGAGCCG TGTGCGTTTC 
AGTGACCCCG AGGGCACCGT GCTGTTCGAG CACCCAGTGC AGAAGAGCGC AGCCCCTGAG 
GACGGCATGC TCTGTGGGAT GTGGAGGACA GTGTCCAAAG CCAACATACA GCTGCTGCGT 
GCGGAGCAGC TCCGTGTGTC CCTCATCACC CGTGCGCAGC CCTCCGGAGA GGTGCATGGG 
CACATCCTGA AGCACCGGGC GCTCTTTGCA GAGACATTTG GTGCCATCCT GACCTCATCA 
GACCCCGCGC ACCTGGGGGC CGGGGGCATG GCCATGCTGA CGCTGAGCGA CACCGAGAAC 
AACCTACACT TCATCCTCAT GGCCCGTGGG CTGCTGGAGC CCGGTGCTGA GGGTGAGGGT 
GGGCATGGGC GCCTGCAAGG CATGGCATCA CCAGTGGGGG GCTCAAGGGA GTCCCCCTGG 
GTACCGCTGC GGGTCCGCAT CCTGCACCAG GGCCAGACGC TGCGCGAGGC CCATGCCAAC 
ATCACTATGG AGGACCCCGA CTTCGCGGAG GTACTGAGCG ACCTGTCTGC CCATGAGCTG 
CAGTGGTTGG CACAGGGGCA GCTCCGCATC GTGGCTGACA CCCAGGGCCG GCACCCGCGC 
CAGCTGGAGG GCACCATTAC CGCCCGCCGC AGCTGTGACA CCATCCAAAG CGTGCTGTGC 
GGCGCAGATG CATTGCAGCC CACCAAGACG GGGGCGGTGG GCTCGGCCAA GCTGGCGCTG 
CATGAGAACG GCACGCTGGA GTACCAGGTG CGGGTGGTGG GCACGGCCAG CGAGGTGGTG 
GGGGTCACAC TGGAGACCAA ACCGCGGCGG AAGAACAGGA GGAACGTCCT CTTCGACATG 
ACACCCAGCT ATAGGGCTGG GATGGCTCAA GGAGTGTGGC ACAGCCCCAG CGCCCGCGAT 
GTGCACATGC TGCTGCAGAA TGAGCTCTTC CTCAACGTGG CCACCAAGGA CTGGGCAGAG 
GGAGAGCTGC GGGGCCAGGT CATCTCCCTG CCCTACAGTG GGCTCCTGGC ACGCTACACA 
GAGATGCCGG TGCCGCTGGC AGGGCAGCTG GTATCCCCCC CAGTGAGCAG CGGGGCCGGG 
GGCCACGCGT GGCTGTCACT GGATGAGCAC TGCCACCTGC ACTACGAGAT AGTGGTGGCG 
GGTCTGGGCC GCCCGGCCGA TGGCACAGTC AGTGCCCAGC TGCACGGCGT GGCCGAGCTG 
GGCGAGATGG GCACTCGGCC GCACCTGCAC AAGCGGATGC TCAAGGGGTT CTATGGCACT 
GAGGCTCAGG GGGTGGTGAA GGACCTGGAT GCTGAGCTGC TGCAGCACCT GGCACAGGGC 
ACCGCATTCC TGCAAGTCAG CACCAAAGCA CACCCGCGTG GGGAGATGCG GGGATGGGTG 
CACATCCCCA ACCGCTGTCA GCCAGGAGGG GCCCGCCTGT CCACCGGGGA GGCTGAGCTC 
TCAGAGGGCC CTAAGGGCAG GAATGTGGAA CAGCTGAAAA AGGACCCCAA CTCCTGCTTC 
TTCGAGGGGC AGCACCATGC ACACGGCACC CGCTGGGCGC CTGACTACGA CAAGAAGTGC 
TCCATCTGCA GCTGCCAGAA GCGCACGGTC ATCTGCGATC CCATCCTGTG CCAGCCCCTC 
AACTGTACCC GCCAGGTGCA CCCTGAGGAG CTGTGCTGCC CCATCTGTGA AGAGAAGAAG 
ATGGAGCAGG AGGAGCTGAA GCTGGAACGG GCACGGGACA GCAGCGAGGG CTGCTACTTT 
GATGGGGACA AGACATGGCG AGGCTCTGGC ACCCGCTGGC ACCCTGTCGT GCCGCCGTTT 
GGCCTCATCA AATGTGCCAT CTGCACCTGC AAGGGCACCA CGGGTGAAGT GCACTGCGAG 
AAGGTGCAGT GTCCGCGCCT CACCTGTGCC AACCCTGTGC GCGTCAGCCC CTCCGACTGC 
TGCAAGCAGT GCCCAGCCCC TGAGAAGAGC ATCCCTGAGC TGACTGATGG CATGCAGGCG 
GACGGACCCC GTGCGTGCCG CTTTGGGCGC CGCTGGTACC TCAACAACGA GAGCTGGCAC 
CCCTCAGTGC CACCCTTTGG GGAGATGAAG TGCATCCTGT GCTGGTGTGT GTCAGGAGAG 
ACCCACTGCC AGCGCCAGGA GTGCCCGCCA GATGCCTGTG CCAGCCCCAC CAGGAGGGAC 
AACCCCTGCT GTGCCAAGTG TCGCGCTCCG GATGCACCCT CAGAAAAGAT GTACGAAGCC 
ACAGCAGAGG CATGGAGCCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRD ( XM_015291373.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146859.1 Gallus gallus chordin (CHRD), transcript variant X2, mRNA.

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CloneID	OGa39871
Clone ID Related Accession (Same CDS sequence)	XM_015291373.2 , XM_015291373.1
Accession Version	XM_015291373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	chordin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGT TTCCCCCATG CCAAGGCCGC GGCTCTCCCA GCTTTCCTTC CTTCCCGGGA 
AAGGGGAGGG AGCTTCCCCT GTTGCTTCCC CACATCCCCG CAGCCGAGCC AGGAGACTGT 
GACGAGCTCC GGGTGCGCGG AGCCAGCGGC GGATTCCTGG CACCGGCGGG GCGGCCGACA 
GCCATCGCCC GCCTCCTCCT CCCCGCTCTG CCCCGCTCGC TCCCCGCCGT TCCCCCCGGG 
CTGCGCCACG GTGGTGGGCA CCGGGCGGTG ACGCTGGCAC GGCCACGCAG CCCCCAGCCC 
TCCCCTGCCA CCGGATCCCA GGCCAACAGG GACAACGGGA GAGAGGAGCA GGGACAGGCC 
TTCCCATGGT GTCTGCTCCC TGCCCACCAC CGCTGCTGCC CTCATCAGCC CGGGCACATG 
TGGGTGAAGA GCAGGATCCG TGCTGGGTGC TGTTCCCATA CCCTCGGCCG CGGCACCTCC 
CTGGGACCCC AGGTCTTGTC CCCACGCTCG TGGGAACGGT CCTGTGGTCC TGCTCTGGGA 
TTGCCATCTG CACCAGGATT TGGGGATGCC AAAGGCACAT GTTGTGGGGG CTCGCAGCTC 
CCGCTTGCCC ATGCCGCCGC CTATAAAGCG GGGCCGGGGC CGGCGGCCGG GCAGAGGCGG 
TGGCAGCGGA GGCAGCAGGC AGCGCCCGGC CCCCCCTCCC CGCCCCCGGC CGCCCGGTTC 
CCCCGGTTCC CCGGCACCAT GCGCACCGCC CTGCTGCTGC TCGCCCTGCT CGCCCTGCCC 
GTCCGCACCG CCCGCCCCAA GCTCGCCCTG CCCATCCGGC CCGACAGCGA GCCGCTGCCC 
CCCGGGGGGG CGGCAGGTTG TGCCTTCGGG GGACGTTTCT ACGCGCTGGA GGAGACGTGG 
CACCCCGACC TGGGGGAGCC CTTTGGGGTG ATGCGCTGCG TGATCTGCCA CTGCGAGACG 
AGGAATCGCC GAGGGAAACC AGTGGGGAAA GTGAACTGCA AGAACATGAA GCAGGATTGC 
CCCGTTCCCA CCTGCCCCCG TGCCACGCTG TTGCCCGGGC ACTGCTGCCA TACCTGTCCC 
AAAGCCTTGC CAGGAGCCCC GGAGAAGAGC TACAAGCCAC CTTTTGACAC CTTTGAGTAC 
TTCCAGGACA AGGAGGACGA GCTGGACAAG CCGTACAATG ACCGCTCCTA CCTCAGCTCT 
GAGGGTTCGG CACGTGACGA TGCCCGCACA GAATTCGTGG CCCTGCTGAC GAGCGGCCCC 
GAGCCGTGGC ACCCCACATC CAGCGCGGTG GCCAAGGCTC GCTTCACCCT GCTGCGCTCC 
TACCTGCTCT TCTCCATCAG CTACGAGCGG CTGGGCCGGC CGAGCCGTGT GCGTTTCAGT 
GACCCCGAGG GCACCGTGCT GTTCGAGCAC CCAGTGCAGA AGAGCGCAGC CCCTGAGGAC 
GGCATGCTCT GTGGGATGTG GAGGACAGTG TCCAAAGCCA ACATACAGCT GCTGCGTGCG 
GAGCAGCTCC GTGTGTCCCT CATCACCCGT GCGCAGCCCT CCGGAGAGGT GCATGGGCAC 
ATCCTGAAGC ACCGGGCGCT CTTTGCAGAG ACATTTGGTG CCATCCTGAC CTCATCAGAC 
CCCGCGCACC TGGGGGCCGG GGGCATGGCC ATGCTGACGC TGAGCGACAC CGAGAACAAC 
CTACACTTCA TCCTCATGGC CCGTGGGCTG CTGGAGCCCG GTGCTGAGGG TGAGGGTGGG 
CATGGGCGCC TGCAAGGCAT GGCATCACCA GTGGGGGGCT CAAGGGAGTC CCCCTGGGTA 
CCGCTGCGGG TCCGCATCCT GCACCAGGGC CAGACGCTGC GCGAGGCCCA TGCCAACATC 
ACTATGGAGG ACCCCGACTT CGCGGAGGTA CTGAGCGACC TGTCTGCCCA TGAGCTGCAG 
TGGTTGGCAC AGGGGCAGCT CCGCATCGTG GCTGACACCC AGGGCCGGCA CCCGCGCCAG 
CTGGAGGGCA CCATTACCGC CCGCCGCAGC TGTGACACCA TCCAAAGCGT GCTGTGCGGC 
GCAGATGCAT TGCAGCCCAC CAAGACGGGG GCGGTGGGCT CGGCCAAGCT GGCGCTGCAT 
GAGAACGGCA CGCTGGAGTA CCAGGTGCGG GTGGTGGGCA CGGCCAGCGA GGTGGTGGGG 
GTCACACTGG AGACCAAACC GCGGCGGAAG AACAGGAGGA ACGTCCTCTT CGACATGACA 
CCCAGCTATA GGGCTGGGAT GGCTCAAGGA GTGTGGCACA GCCCCAGCGC CCGCGATGTG 
CACATGCTGC TGCAGAATGA GCTCTTCCTC AACGTGGCCA CCAAGGACTG GGCAGAGGGA 
GAGCTGCGGG GCCAGGTCAT CTCCCTGCCC TACAGTGGGC TCCTGGCACG CTACACAGAG 
ATGCCGGTGC CGCTGGCAGG GCAGCTGGTA TCCCCCCCAG TGAGCAGCGG GGCCGGGGGC 
CACGCGTGGC TGTCACTGGA TGAGCACTGC CACCTGCACT ACGAGATAGT GGTGGCGGGT 
CTGGGCCGCC CGGCCGATGG CACAGTCAGT GCCCAGCTGC ACGGCGTGGC CGAGCTGGGC 
GAGATGGGCA CTCGGCCGCA CCTGCACAAG CGGATGCTCA AGGGGTTCTA TGGCACTGAG 
GCTCAGGGGG TGGTGAAGGA CCTGGATGCT GAGCTGCTGC AGCACCTGGC ACAGGGCACC 
GCATTCCTGC AAGTCAGCAC CAAAGCACAC CCGCGTGGGG AGATGCGGGG ATGGGTGCAC 
ATCCCCAACC GCTGTCAGCC AGGAGGGGCC CGCCTGTCCA CCGGGGAGGC TGAGCTCTCA 
GAGGGCCCTA AGGGCAGGAA TGTGGAACAG CTGAAAAAGG ACCCCAACTC CTGCTTCTTC 
GAGGGGCAGC ACCATGCACA CGGCACCCGC TGGGCGCCTG ACTACGACAA GAAGTGCTCC 
ATCTGCAGCT GCCAGAAGCG CACGGTCATC TGCGATCCCA TCCTGTGCCA GCCCCTCAAC 
TGTACCCGCC AGGTGCACCC TGAGGAGCTG TGCTGCCCCA TCTGTGAAGA GAAGAAGATG 
GAGCAGGAGG AGCTGAAGCT GGAACGGGCA CGGGACAGCA GCGAGGGCTG CTACTTTGAT 
GGGGACAAGA CATGGCGAGG CTCTGGCACC CGCTGGCACC CTGTCGTGCC GCCGTTTGGC 
CTCATCAAAT GTGCCATCTG CACCTGCAAG GGCACCACGG GTGAAGTGCA CTGCGAGAAG 
GTGCAGTGTC CGCGCCTCAC CTGTGCCAAC CCTGTGCGCG TCAGCCCCTC CGACTGCTGC 
AAGCAGTGCC CAGCCCCTGA GAAGAGCATC CCTGAGCTGA CTGATGGCAT GCAGGCGGAC 
GGACCCCGTG CGTGCCGCTT TGGGCGCCGC TGGTACCTCA ACAACGAGAG CTGGCACCCC 
TCAGTGCCAC CCTTTGGGGA GATGAAGTGC ATCCTGTGCT GGTGTGTGTC AGGAGAGACC 
CACTGCCAGC GCCAGGAGTG CCCGCCAGAT GCCTGTGCCA GCCCCACCAG GAGGGACAAC 
CCCTGCTGTG CCAAGTGTCG CGCTCCGGAT GCACCCTCAG AAAAGATGTA CGAAGCCACA 
GCAGAGGCAT GGAGCCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146859.1
CDS	35..3655
Translation	MPQFPPCQGRGSPSFPSFPGKGRELPLLLPHIPAAEPGDCDELRVRGASGGFLAPAGRPTAIARLLLPALPRSLPAVPPGLRHGGGHRAVTLARPRSPQPSPATGSQANRDNGREEQGQAFPWCLLPAHHRCCPHQPGHMWVKSRIRAGCCSHTLGRGTSLGPQVLSPRSWERSCGPALGLPSAPGFGDAKGTCCGGSQLPLAHAAAYKAGPGPAAGQRRWQRRQQAAPGPPSPPPAARFPRFPGTMRTALLLLALLALPVRTARPKLALPIRPDSEPLPPGGAAGCAFGGRFYALEETWHPDLGEPFGVMRCVICHCETRNRRGKPVGKVNCKNMKQDCPVPTCPRATLLPGHCCHTCPKALPGAPEKSYKPPFDTFEYFQDKEDELDKPYNDRSYLSSEGSARDDARTEFVALLTSGPEPWHPTSSAVAKARFTLLRSYLLFSISYERLGRPSRVRFSDPEGTVLFEHPVQKSAAPEDGMLCGMWRTVSKANIQLLRAEQLRVSLITRAQPSGEVHGHILKHRALFAETFGAILTSSDPAHLGAGGMAMLTLSDTENNLHFILMARGLLEPGAEGEGGHGRLQGMASPVGGSRESPWVPLRVRILHQGQTLREAHANITMEDPDFAEVLSDLSAHELQWLAQGQLRIVADTQGRHPRQLEGTITARRSCDTIQSVLCGADALQPTKTGAVGSAKLALHENGTLEYQVRVVGTASEVVGVTLETKPRRKNRRNVLFDMTPSYRAGMAQGVWHSPSARDVHMLLQNELFLNVATKDWAEGELRGQVISLPYSGLLARYTEMPVPLAGQLVSPPVSSGAGGHAWLSLDEHCHLHYEIVVAGLGRPADGTVSAQLHGVAELGEMGTRPHLHKRMLKGFYGTEAQGVVKDLDAELLQHLAQGTAFLQVSTKAHPRGEMRGWVHIPNRCQPGGARLSTGEAELSEGPKGRNVEQLKKDPNSCFFEGQHHAHGTRWAPDYDKKCSICSCQKRTVICDPILCQPLNCTRQVHPEELCCPICEEKKMEQEELKLERARDSSEGCYFDGDKTWRGSGTRWHPVVPPFGLIKCAICTCKGTTGEVHCEKVQCPRLTCANPVRVSPSDCCKQCPAPEKSIPELTDGMQADGPRACRFGRRWYLNNESWHPSVPPFGEMKCILCWCVSGETHCQRQECPPDACASPTRRDNPCCAKCRAPDAPSEKMYEATAEAWSR

Target ORF information:

RefSeq Version	XM_015291373.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chordin (CHRD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291373.2

ORF Insert Sequence:

ATGCCTCAGT TTCCCCCATG CCAAGGCCGC GGCTCTCCCA GCTTTCCTTC CTTCCCGGGA 
AAGGGGAGGG AGCTTCCCCT GTTGCTTCCC CACATCCCCG CAGCCGAGCC AGGAGACTGT 
GACGAGCTCC GGGTGCGCGG AGCCAGCGGC GGATTCCTGG CACCGGCGGG GCGGCCGACA 
GCCATCGCCC GCCTCCTCCT CCCCGCTCTG CCCCGCTCGC TCCCCGCCGT TCCCCCCGGG 
CTGCGCCACG GTGGTGGGCA CCGGGCGGTG ACGCTGGCAC GGCCACGCAG CCCCCAGCCC 
TCCCCTGCCA CCGGATCCCA GGCCAACAGG GACAACGGGA GAGAGGAGCA GGGACAGGCC 
TTCCCATGGT GTCTGCTCCC TGCCCACCAC CGCTGCTGCC CTCATCAGCC CGGGCACATG 
TGGGTGAAGA GCAGGATCCG TGCTGGGTGC TGTTCCCATA CCCTCGGCCG CGGCACCTCC 
CTGGGACCCC AGGTCTTGTC CCCACGCTCG TGGGAACGGT CCTGTGGTCC TGCTCTGGGA 
TTGCCATCTG CACCAGGATT TGGGGATGCC AAAGGCACAT GTTGTGGGGG CTCGCAGCTC 
CCGCTTGCCC ATGCCGCCGC CTATAAAGCG GGGCCGGGGC CGGCGGCCGG GCAGAGGCGG 
TGGCAGCGGA GGCAGCAGGC AGCGCCCGGC CCCCCCTCCC CGCCCCCGGC CGCCCGGTTC 
CCCCGGTTCC CCGGCACCAT GCGCACCGCC CTGCTGCTGC TCGCCCTGCT CGCCCTGCCC 
GTCCGCACCG CCCGCCCCAA GCTCGCCCTG CCCATCCGGC CCGACAGCGA GCCGCTGCCC 
CCCGGGGGGG CGGCAGGTTG TGCCTTCGGG GGACGTTTCT ACGCGCTGGA GGAGACGTGG 
CACCCCGACC TGGGGGAGCC CTTTGGGGTG ATGCGCTGCG TGATCTGCCA CTGCGAGACG 
AGGAATCGCC GAGGGAAACC AGTGGGGAAA GTGAACTGCA AGAACATGAA GCAGGATTGC 
CCCGTTCCCA CCTGCCCCCG TGCCACGCTG TTGCCCGGGC ACTGCTGCCA TACCTGTCCC 
AAAGCCTTGC CAGGAGCCCC GGAGAAGAGC TACAAGCCAC CTTTTGACAC CTTTGAGTAC 
TTCCAGGACA AGGAGGACGA GCTGGACAAG CCGTACAATG ACCGCTCCTA CCTCAGCTCT 
GAGGGTTCGG CACGTGACGA TGCCCGCACA GAATTCGTGG CCCTGCTGAC GAGCGGCCCC 
GAGCCGTGGC ACCCCACATC CAGCGCGGTG GCCAAGGCTC GCTTCACCCT GCTGCGCTCC 
TACCTGCTCT TCTCCATCAG CTACGAGCGG CTGGGCCGGC CGAGCCGTGT GCGTTTCAGT 
GACCCCGAGG GCACCGTGCT GTTCGAGCAC CCAGTGCAGA AGAGCGCAGC CCCTGAGGAC 
GGCATGCTCT GTGGGATGTG GAGGACAGTG TCCAAAGCCA ACATACAGCT GCTGCGTGCG 
GAGCAGCTCC GTGTGTCCCT CATCACCCGT GCGCAGCCCT CCGGAGAGGT GCATGGGCAC 
ATCCTGAAGC ACCGGGCGCT CTTTGCAGAG ACATTTGGTG CCATCCTGAC CTCATCAGAC 
CCCGCGCACC TGGGGGCCGG GGGCATGGCC ATGCTGACGC TGAGCGACAC CGAGAACAAC 
CTACACTTCA TCCTCATGGC CCGTGGGCTG CTGGAGCCCG GTGCTGAGGG TGAGGGTGGG 
CATGGGCGCC TGCAAGGCAT GGCATCACCA GTGGGGGGCT CAAGGGAGTC CCCCTGGGTA 
CCGCTGCGGG TCCGCATCCT GCACCAGGGC CAGACGCTGC GCGAGGCCCA TGCCAACATC 
ACTATGGAGG ACCCCGACTT CGCGGAGGTA CTGAGCGACC TGTCTGCCCA TGAGCTGCAG 
TGGTTGGCAC AGGGGCAGCT CCGCATCGTG GCTGACACCC AGGGCCGGCA CCCGCGCCAG 
CTGGAGGGCA CCATTACCGC CCGCCGCAGC TGTGACACCA TCCAAAGCGT GCTGTGCGGC 
GCAGATGCAT TGCAGCCCAC CAAGACGGGG GCGGTGGGCT CGGCCAAGCT GGCGCTGCAT 
GAGAACGGCA CGCTGGAGTA CCAGGTGCGG GTGGTGGGCA CGGCCAGCGA GGTGGTGGGG 
GTCACACTGG AGACCAAACC GCGGCGGAAG AACAGGAGGA ACGTCCTCTT CGACATGACA 
CCCAGCTATA GGGCTGGGAT GGCTCAAGGA GTGTGGCACA GCCCCAGCGC CCGCGATGTG 
CACATGCTGC TGCAGAATGA GCTCTTCCTC AACGTGGCCA CCAAGGACTG GGCAGAGGGA 
GAGCTGCGGG GCCAGGTCAT CTCCCTGCCC TACAGTGGGC TCCTGGCACG CTACACAGAG 
ATGCCGGTGC CGCTGGCAGG GCAGCTGGTA TCCCCCCCAG TGAGCAGCGG GGCCGGGGGC 
CACGCGTGGC TGTCACTGGA TGAGCACTGC CACCTGCACT ACGAGATAGT GGTGGCGGGT 
CTGGGCCGCC CGGCCGATGG CACAGTCAGT GCCCAGCTGC ACGGCGTGGC CGAGCTGGGC 
GAGATGGGCA CTCGGCCGCA CCTGCACAAG CGGATGCTCA AGGGGTTCTA TGGCACTGAG 
GCTCAGGGGG TGGTGAAGGA CCTGGATGCT GAGCTGCTGC AGCACCTGGC ACAGGGCACC 
GCATTCCTGC AAGTCAGCAC CAAAGCACAC CCGCGTGGGG AGATGCGGGG ATGGGTGCAC 
ATCCCCAACC GCTGTCAGCC AGGAGGGGCC CGCCTGTCCA CCGGGGAGGC TGAGCTCTCA 
GAGGGCCCTA AGGGCAGGAA TGTGGAACAG CTGAAAAAGG ACCCCAACTC CTGCTTCTTC 
GAGGGGCAGC ACCATGCACA CGGCACCCGC TGGGCGCCTG ACTACGACAA GAAGTGCTCC 
ATCTGCAGCT GCCAGAAGCG CACGGTCATC TGCGATCCCA TCCTGTGCCA GCCCCTCAAC 
TGTACCCGCC AGGTGCACCC TGAGGAGCTG TGCTGCCCCA TCTGTGAAGA GAAGAAGATG 
GAGCAGGAGG AGCTGAAGCT GGAACGGGCA CGGGACAGCA GCGAGGGCTG CTACTTTGAT 
GGGGACAAGA CATGGCGAGG CTCTGGCACC CGCTGGCACC CTGTCGTGCC GCCGTTTGGC 
CTCATCAAAT GTGCCATCTG CACCTGCAAG GGCACCACGG GTGAAGTGCA CTGCGAGAAG 
GTGCAGTGTC CGCGCCTCAC CTGTGCCAAC CCTGTGCGCG TCAGCCCCTC CGACTGCTGC 
AAGCAGTGCC CAGCCCCTGA GAAGAGCATC CCTGAGCTGA CTGATGGCAT GCAGGCGGAC 
GGACCCCGTG CGTGCCGCTT TGGGCGCCGC TGGTACCTCA ACAACGAGAG CTGGCACCCC 
TCAGTGCCAC CCTTTGGGGA GATGAAGTGC ATCCTGTGCT GGTGTGTGTC AGGAGAGACC 
CACTGCCAGC GCCAGGAGTG CCCGCCAGAT GCCTGTGCCA GCCCCACCAG GAGGGACAAC 
CCCTGCTGTG CCAAGTGTCG CGCTCCGGAT GCACCCTCAG AAAAGATGTA CGAAGCCACA 
GCAGAGGCAT GGAGCCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRD ( XM_015291373.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146859.1 Gallus gallus chordin (CHRD), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa39871
Clone ID Related Accession (Same CDS sequence)	XM_015291373.2 , XM_015291373.1
Accession Version	XM_015291373.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	chordin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGT TTCCCCCATG CCAAGGCCGC GGCTCTCCCA GCTTTCCTTC CTTCCCGGGA 
AAGGGGAGGG AGCTTCCCCT GTTGCTTCCC CACATCCCCG CAGCCGAGCC AGGAGACTGT 
GACGAGCTCC GGGTGCGCGG AGCCAGCGGC GGATTCCTGG CACCGGCGGG GCGGCCGACA 
GCCATCGCCC GCCTCCTCCT CCCCGCTCTG CCCCGCTCGC TCCCCGCCGT TCCCCCCGGG 
CTGCGCCACG GTGGTGGGCA CCGGGCGGTG ACGCTGGCAC GGCCACGCAG CCCCCAGCCC 
TCCCCTGCCA CCGGATCCCA GGCCAACAGG GACAACGGGA GAGAGGAGCA GGGACAGGCC 
TTCCCATGGT GTCTGCTCCC TGCCCACCAC CGCTGCTGCC CTCATCAGCC CGGGCACATG 
TGGGTGAAGA GCAGGATCCG TGCTGGGTGC TGTTCCCATA CCCTCGGCCG CGGCACCTCC 
CTGGGACCCC AGGTCTTGTC CCCACGCTCG TGGGAACGGT CCTGTGGTCC TGCTCTGGGA 
TTGCCATCTG CACCAGGATT TGGGGATGCC AAAGGCACAT GTTGTGGGGG CTCGCAGCTC 
CCGCTTGCCC ATGCCGCCGC CTATAAAGCG GGGCCGGGGC CGGCGGCCGG GCAGAGGCGG 
TGGCAGCGGA GGCAGCAGGC AGCGCCCGGC CCCCCCTCCC CGCCCCCGGC CGCCCGGTTC 
CCCCGGTTCC CCGGCACCAT GCGCACCGCC CTGCTGCTGC TCGCCCTGCT CGCCCTGCCC 
GTCCGCACCG CCCGCCCCAA GCTCGCCCTG CCCATCCGGC CCGACAGCGA GCCGCTGCCC 
CCCGGGGGGG CGGCAGGTTG TGCCTTCGGG GGACGTTTCT ACGCGCTGGA GGAGACGTGG 
CACCCCGACC TGGGGGAGCC CTTTGGGGTG ATGCGCTGCG TGATCTGCCA CTGCGAGACG 
AGGAATCGCC GAGGGAAACC AGTGGGGAAA GTGAACTGCA AGAACATGAA GCAGGATTGC 
CCCGTTCCCA CCTGCCCCCG TGCCACGCTG TTGCCCGGGC ACTGCTGCCA TACCTGTCCC 
AAAGCCTTGC CAGGAGCCCC GGAGAAGAGC TACAAGCCAC CTTTTGACAC CTTTGAGTAC 
TTCCAGGACA AGGAGGACGA GCTGGACAAG CCGTACAATG ACCGCTCCTA CCTCAGCTCT 
GAGGGTTCGG CACGTGACGA TGCCCGCACA GAATTCGTGG CCCTGCTGAC GAGCGGCCCC 
GAGCCGTGGC ACCCCACATC CAGCGCGGTG GCCAAGGCTC GCTTCACCCT GCTGCGCTCC 
TACCTGCTCT TCTCCATCAG CTACGAGCGG CTGGGCCGGC CGAGCCGTGT GCGTTTCAGT 
GACCCCGAGG GCACCGTGCT GTTCGAGCAC CCAGTGCAGA AGAGCGCAGC CCCTGAGGAC 
GGCATGCTCT GTGGGATGTG GAGGACAGTG TCCAAAGCCA ACATACAGCT GCTGCGTGCG 
GAGCAGCTCC GTGTGTCCCT CATCACCCGT GCGCAGCCCT CCGGAGAGGT GCATGGGCAC 
ATCCTGAAGC ACCGGGCGCT CTTTGCAGAG ACATTTGGTG CCATCCTGAC CTCATCAGAC 
CCCGCGCACC TGGGGGCCGG GGGCATGGCC ATGCTGACGC TGAGCGACAC CGAGAACAAC 
CTACACTTCA TCCTCATGGC CCGTGGGCTG CTGGAGCCCG GTGCTGAGGG TGAGGGTGGG 
CATGGGCGCC TGCAAGGCAT GGCATCACCA GTGGGGGGCT CAAGGGAGTC CCCCTGGGTA 
CCGCTGCGGG TCCGCATCCT GCACCAGGGC CAGACGCTGC GCGAGGCCCA TGCCAACATC 
ACTATGGAGG ACCCCGACTT CGCGGAGGTA CTGAGCGACC TGTCTGCCCA TGAGCTGCAG 
TGGTTGGCAC AGGGGCAGCT CCGCATCGTG GCTGACACCC AGGGCCGGCA CCCGCGCCAG 
CTGGAGGGCA CCATTACCGC CCGCCGCAGC TGTGACACCA TCCAAAGCGT GCTGTGCGGC 
GCAGATGCAT TGCAGCCCAC CAAGACGGGG GCGGTGGGCT CGGCCAAGCT GGCGCTGCAT 
GAGAACGGCA CGCTGGAGTA CCAGGTGCGG GTGGTGGGCA CGGCCAGCGA GGTGGTGGGG 
GTCACACTGG AGACCAAACC GCGGCGGAAG AACAGGAGGA ACGTCCTCTT CGACATGACA 
CCCAGCTATA GGGCTGGGAT GGCTCAAGGA GTGTGGCACA GCCCCAGCGC CCGCGATGTG 
CACATGCTGC TGCAGAATGA GCTCTTCCTC AACGTGGCCA CCAAGGACTG GGCAGAGGGA 
GAGCTGCGGG GCCAGGTCAT CTCCCTGCCC TACAGTGGGC TCCTGGCACG CTACACAGAG 
ATGCCGGTGC CGCTGGCAGG GCAGCTGGTA TCCCCCCCAG TGAGCAGCGG GGCCGGGGGC 
CACGCGTGGC TGTCACTGGA TGAGCACTGC CACCTGCACT ACGAGATAGT GGTGGCGGGT 
CTGGGCCGCC CGGCCGATGG CACAGTCAGT GCCCAGCTGC ACGGCGTGGC CGAGCTGGGC 
GAGATGGGCA CTCGGCCGCA CCTGCACAAG CGGATGCTCA AGGGGTTCTA TGGCACTGAG 
GCTCAGGGGG TGGTGAAGGA CCTGGATGCT GAGCTGCTGC AGCACCTGGC ACAGGGCACC 
GCATTCCTGC AAGTCAGCAC CAAAGCACAC CCGCGTGGGG AGATGCGGGG ATGGGTGCAC 
ATCCCCAACC GCTGTCAGCC AGGAGGGGCC CGCCTGTCCA CCGGGGAGGC TGAGCTCTCA 
GAGGGCCCTA AGGGCAGGAA TGTGGAACAG CTGAAAAAGG ACCCCAACTC CTGCTTCTTC 
GAGGGGCAGC ACCATGCACA CGGCACCCGC TGGGCGCCTG ACTACGACAA GAAGTGCTCC 
ATCTGCAGCT GCCAGAAGCG CACGGTCATC TGCGATCCCA TCCTGTGCCA GCCCCTCAAC 
TGTACCCGCC AGGTGCACCC TGAGGAGCTG TGCTGCCCCA TCTGTGAAGA GAAGAAGATG 
GAGCAGGAGG AGCTGAAGCT GGAACGGGCA CGGGACAGCA GCGAGGGCTG CTACTTTGAT 
GGGGACAAGA CATGGCGAGG CTCTGGCACC CGCTGGCACC CTGTCGTGCC GCCGTTTGGC 
CTCATCAAAT GTGCCATCTG CACCTGCAAG GGCACCACGG GTGAAGTGCA CTGCGAGAAG 
GTGCAGTGTC CGCGCCTCAC CTGTGCCAAC CCTGTGCGCG TCAGCCCCTC CGACTGCTGC 
AAGCAGTGCC CAGCCCCTGA GAAGAGCATC CCTGAGCTGA CTGATGGCAT GCAGGCGGAC 
GGACCCCGTG CGTGCCGCTT TGGGCGCCGC TGGTACCTCA ACAACGAGAG CTGGCACCCC 
TCAGTGCCAC CCTTTGGGGA GATGAAGTGC ATCCTGTGCT GGTGTGTGTC AGGAGAGACC 
CACTGCCAGC GCCAGGAGTG CCCGCCAGAT GCCTGTGCCA GCCCCACCAG GAGGGACAAC 
CCCTGCTGTG CCAAGTGTCG CGCTCCGGAT GCACCCTCAG AAAAGATGTA CGAAGCCACA 
GCAGAGGCAT GGAGCCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146859.1
CDS	19..3639
Translation	MPQFPPCQGRGSPSFPSFPGKGRELPLLLPHIPAAEPGDCDELRVRGASGGFLAPAGRPTAIARLLLPALPRSLPAVPPGLRHGGGHRAVTLARPRSPQPSPATGSQANRDNGREEQGQAFPWCLLPAHHRCCPHQPGHMWVKSRIRAGCCSHTLGRGTSLGPQVLSPRSWERSCGPALGLPSAPGFGDAKGTCCGGSQLPLAHAAAYKAGPGPAAGQRRWQRRQQAAPGPPSPPPAARFPRFPGTMRTALLLLALLALPVRTARPKLALPIRPDSEPLPPGGAAGCAFGGRFYALEETWHPDLGEPFGVMRCVICHCETRNRRGKPVGKVNCKNMKQDCPVPTCPRATLLPGHCCHTCPKALPGAPEKSYKPPFDTFEYFQDKEDELDKPYNDRSYLSSEGSARDDARTEFVALLTSGPEPWHPTSSAVAKARFTLLRSYLLFSISYERLGRPSRVRFSDPEGTVLFEHPVQKSAAPEDGMLCGMWRTVSKANIQLLRAEQLRVSLITRAQPSGEVHGHILKHRALFAETFGAILTSSDPAHLGAGGMAMLTLSDTENNLHFILMARGLLEPGAEGEGGHGRLQGMASPVGGSRESPWVPLRVRILHQGQTLREAHANITMEDPDFAEVLSDLSAHELQWLAQGQLRIVADTQGRHPRQLEGTITARRSCDTIQSVLCGADALQPTKTGAVGSAKLALHENGTLEYQVRVVGTASEVVGVTLETKPRRKNRRNVLFDMTPSYRAGMAQGVWHSPSARDVHMLLQNELFLNVATKDWAEGELRGQVISLPYSGLLARYTEMPVPLAGQLVSPPVSSGAGGHAWLSLDEHCHLHYEIVVAGLGRPADGTVSAQLHGVAELGEMGTRPHLHKRMLKGFYGTEAQGVVKDLDAELLQHLAQGTAFLQVSTKAHPRGEMRGWVHIPNRCQPGGARLSTGEAELSEGPKGRNVEQLKKDPNSCFFEGQHHAHGTRWAPDYDKKCSICSCQKRTVICDPILCQPLNCTRQVHPEELCCPICEEKKMEQEELKLERARDSSEGCYFDGDKTWRGSGTRWHPVVPPFGLIKCAICTCKGTTGEVHCEKVQCPRLTCANPVRVSPSDCCKQCPAPEKSIPELTDGMQADGPRACRFGRRWYLNNESWHPSVPPFGEMKCILCWCVSGETHCQRQECPPDACASPTRRDNPCCAKCRAPDAPSEKMYEATAEAWSR

Target ORF information:

RefSeq Version	XM_015291373.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chordin (CHRD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291373.1

ORF Insert Sequence:

ATGCCTCAGT TTCCCCCATG CCAAGGCCGC GGCTCTCCCA GCTTTCCTTC CTTCCCGGGA 
AAGGGGAGGG AGCTTCCCCT GTTGCTTCCC CACATCCCCG CAGCCGAGCC AGGAGACTGT 
GACGAGCTCC GGGTGCGCGG AGCCAGCGGC GGATTCCTGG CACCGGCGGG GCGGCCGACA 
GCCATCGCCC GCCTCCTCCT CCCCGCTCTG CCCCGCTCGC TCCCCGCCGT TCCCCCCGGG 
CTGCGCCACG GTGGTGGGCA CCGGGCGGTG ACGCTGGCAC GGCCACGCAG CCCCCAGCCC 
TCCCCTGCCA CCGGATCCCA GGCCAACAGG GACAACGGGA GAGAGGAGCA GGGACAGGCC 
TTCCCATGGT GTCTGCTCCC TGCCCACCAC CGCTGCTGCC CTCATCAGCC CGGGCACATG 
TGGGTGAAGA GCAGGATCCG TGCTGGGTGC TGTTCCCATA CCCTCGGCCG CGGCACCTCC 
CTGGGACCCC AGGTCTTGTC CCCACGCTCG TGGGAACGGT CCTGTGGTCC TGCTCTGGGA 
TTGCCATCTG CACCAGGATT TGGGGATGCC AAAGGCACAT GTTGTGGGGG CTCGCAGCTC 
CCGCTTGCCC ATGCCGCCGC CTATAAAGCG GGGCCGGGGC CGGCGGCCGG GCAGAGGCGG 
TGGCAGCGGA GGCAGCAGGC AGCGCCCGGC CCCCCCTCCC CGCCCCCGGC CGCCCGGTTC 
CCCCGGTTCC CCGGCACCAT GCGCACCGCC CTGCTGCTGC TCGCCCTGCT CGCCCTGCCC 
GTCCGCACCG CCCGCCCCAA GCTCGCCCTG CCCATCCGGC CCGACAGCGA GCCGCTGCCC 
CCCGGGGGGG CGGCAGGTTG TGCCTTCGGG GGACGTTTCT ACGCGCTGGA GGAGACGTGG 
CACCCCGACC TGGGGGAGCC CTTTGGGGTG ATGCGCTGCG TGATCTGCCA CTGCGAGACG 
AGGAATCGCC GAGGGAAACC AGTGGGGAAA GTGAACTGCA AGAACATGAA GCAGGATTGC 
CCCGTTCCCA CCTGCCCCCG TGCCACGCTG TTGCCCGGGC ACTGCTGCCA TACCTGTCCC 
AAAGCCTTGC CAGGAGCCCC GGAGAAGAGC TACAAGCCAC CTTTTGACAC CTTTGAGTAC 
TTCCAGGACA AGGAGGACGA GCTGGACAAG CCGTACAATG ACCGCTCCTA CCTCAGCTCT 
GAGGGTTCGG CACGTGACGA TGCCCGCACA GAATTCGTGG CCCTGCTGAC GAGCGGCCCC 
GAGCCGTGGC ACCCCACATC CAGCGCGGTG GCCAAGGCTC GCTTCACCCT GCTGCGCTCC 
TACCTGCTCT TCTCCATCAG CTACGAGCGG CTGGGCCGGC CGAGCCGTGT GCGTTTCAGT 
GACCCCGAGG GCACCGTGCT GTTCGAGCAC CCAGTGCAGA AGAGCGCAGC CCCTGAGGAC 
GGCATGCTCT GTGGGATGTG GAGGACAGTG TCCAAAGCCA ACATACAGCT GCTGCGTGCG 
GAGCAGCTCC GTGTGTCCCT CATCACCCGT GCGCAGCCCT CCGGAGAGGT GCATGGGCAC 
ATCCTGAAGC ACCGGGCGCT CTTTGCAGAG ACATTTGGTG CCATCCTGAC CTCATCAGAC 
CCCGCGCACC TGGGGGCCGG GGGCATGGCC ATGCTGACGC TGAGCGACAC CGAGAACAAC 
CTACACTTCA TCCTCATGGC CCGTGGGCTG CTGGAGCCCG GTGCTGAGGG TGAGGGTGGG 
CATGGGCGCC TGCAAGGCAT GGCATCACCA GTGGGGGGCT CAAGGGAGTC CCCCTGGGTA 
CCGCTGCGGG TCCGCATCCT GCACCAGGGC CAGACGCTGC GCGAGGCCCA TGCCAACATC 
ACTATGGAGG ACCCCGACTT CGCGGAGGTA CTGAGCGACC TGTCTGCCCA TGAGCTGCAG 
TGGTTGGCAC AGGGGCAGCT CCGCATCGTG GCTGACACCC AGGGCCGGCA CCCGCGCCAG 
CTGGAGGGCA CCATTACCGC CCGCCGCAGC TGTGACACCA TCCAAAGCGT GCTGTGCGGC 
GCAGATGCAT TGCAGCCCAC CAAGACGGGG GCGGTGGGCT CGGCCAAGCT GGCGCTGCAT 
GAGAACGGCA CGCTGGAGTA CCAGGTGCGG GTGGTGGGCA CGGCCAGCGA GGTGGTGGGG 
GTCACACTGG AGACCAAACC GCGGCGGAAG AACAGGAGGA ACGTCCTCTT CGACATGACA 
CCCAGCTATA GGGCTGGGAT GGCTCAAGGA GTGTGGCACA GCCCCAGCGC CCGCGATGTG 
CACATGCTGC TGCAGAATGA GCTCTTCCTC AACGTGGCCA CCAAGGACTG GGCAGAGGGA 
GAGCTGCGGG GCCAGGTCAT CTCCCTGCCC TACAGTGGGC TCCTGGCACG CTACACAGAG 
ATGCCGGTGC CGCTGGCAGG GCAGCTGGTA TCCCCCCCAG TGAGCAGCGG GGCCGGGGGC 
CACGCGTGGC TGTCACTGGA TGAGCACTGC CACCTGCACT ACGAGATAGT GGTGGCGGGT 
CTGGGCCGCC CGGCCGATGG CACAGTCAGT GCCCAGCTGC ACGGCGTGGC CGAGCTGGGC 
GAGATGGGCA CTCGGCCGCA CCTGCACAAG CGGATGCTCA AGGGGTTCTA TGGCACTGAG 
GCTCAGGGGG TGGTGAAGGA CCTGGATGCT GAGCTGCTGC AGCACCTGGC ACAGGGCACC 
GCATTCCTGC AAGTCAGCAC CAAAGCACAC CCGCGTGGGG AGATGCGGGG ATGGGTGCAC 
ATCCCCAACC GCTGTCAGCC AGGAGGGGCC CGCCTGTCCA CCGGGGAGGC TGAGCTCTCA 
GAGGGCCCTA AGGGCAGGAA TGTGGAACAG CTGAAAAAGG ACCCCAACTC CTGCTTCTTC 
GAGGGGCAGC ACCATGCACA CGGCACCCGC TGGGCGCCTG ACTACGACAA GAAGTGCTCC 
ATCTGCAGCT GCCAGAAGCG CACGGTCATC TGCGATCCCA TCCTGTGCCA GCCCCTCAAC 
TGTACCCGCC AGGTGCACCC TGAGGAGCTG TGCTGCCCCA TCTGTGAAGA GAAGAAGATG 
GAGCAGGAGG AGCTGAAGCT GGAACGGGCA CGGGACAGCA GCGAGGGCTG CTACTTTGAT 
GGGGACAAGA CATGGCGAGG CTCTGGCACC CGCTGGCACC CTGTCGTGCC GCCGTTTGGC 
CTCATCAAAT GTGCCATCTG CACCTGCAAG GGCACCACGG GTGAAGTGCA CTGCGAGAAG 
GTGCAGTGTC CGCGCCTCAC CTGTGCCAAC CCTGTGCGCG TCAGCCCCTC CGACTGCTGC 
AAGCAGTGCC CAGCCCCTGA GAAGAGCATC CCTGAGCTGA CTGATGGCAT GCAGGCGGAC 
GGACCCCGTG CGTGCCGCTT TGGGCGCCGC TGGTACCTCA ACAACGAGAG CTGGCACCCC 
TCAGTGCCAC CCTTTGGGGA GATGAAGTGC ATCCTGTGCT GGTGTGTGTC AGGAGAGACC 
CACTGCCAGC GCCAGGAGTG CCCGCCAGAT GCCTGTGCCA GCCCCACCAG GAGGGACAAC 
CCCTGCTGTG CCAAGTGTCG CGCTCCGGAT GCACCCTCAG AAAAGATGTA CGAAGCCACA 
GCAGAGGCAT GGAGCCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRD ( XM_015291374.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146860.1 Gallus gallus chordin (CHRD), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa39872
Clone ID Related Accession (Same CDS sequence)	XM_015291374.1 , XM_015291374.2
Accession Version	XM_015291374.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	chordin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGT TTCCCCCATG CCAAGGCCGC GGCTCTCCCA GCTTTCCTTC CTTCCCGGGA 
AAGGGGAGGG AGCTTCCCCT GTTGCTTCCC CACATCCCCG CAGCCGAGCC AGGAGACTGT 
GACGAGCTCC GGGTGCGCGG AGCCAGCGGC GGATTCCTGG CACCGGCGGG GCGGCCGACA 
GCCATCGCCC GCCTCCTCCT CCCCGCTCTG CCCCGCTCGC TCCCCGCCGT TCCCCCCGGG 
CTGCGCCACG GTGGTGGGCA CCGGGCGGTG ACGCTGGCAC GGCCACGCAG CCCCCAGCCC 
TCCCCTGCCA CCGGATCCCA GGCCAACAGG GACAACGGGA GAGAGGAGCA GGGACAGGCC 
TTCCCATGGT GTCTGCTCCC TGCCCACCAC CGCTGCTGCC CTCATCAGCC CGGGCACATG 
TGGGTGAAGA GCAGGATCCG TGCTGGGTGC TGTTCCCATA CCCTCGGCCG CGGCACCTCC 
CTGGGACCCC AGGTCTTGTC CCCACGCTCG TGGGAACGGT CCTGTGGTCC TGCTCTGGGA 
TTGCCATCTG CACCAGGATT TGGGGATGCC AAAGGCACAT GTTGTGGGGG CTCGCAGCTC 
CCGCTTGCCC ATGCCGCCGC CTATAAAGCG GGGCCGGGGC CGGCGGCCGG GCAGAGGCGG 
TGGCAGCGGA GGCAGCAGGC AGCGCCCGGC CCCCCCTCCC CGCCCCCGGC CGCCCGGTTC 
CCCCGGTTCC CCGGCACCAT GCGCACCGCC CTGCTGCTGC TCGCCCTGCT CGCCCTGCCC 
GTCCGCACCG CCCGCCCCAA GCTCGCCCTG CCCATCCGGC CCGACAGCGA GCCGCTGCCC 
CCCGGGGGGG CGGCAGGTTG TGCCTTCGGG GGACGTTTCT ACGCGCTGGA GGAGACGTGG 
CACCCCGACC TGGGGGAGCC CTTTGGGGTG ATGCGCTGCG TGATCTGCCA CTGCGAGACG 
CAGAGGAATC GCCGAGGGAA ACCAGTGGGG AAAGTGAACT GCAAGAACAT GAAGCAGGAT 
TGCCCCGTTC CCACCTGCCC CCGTGCCACG CTGTTGCCCG GGCACTGCTG CCATACCTGT 
CCCAAAGCCT TGCCAGGAGC CCCGGAGAAG AGCTACAAGC CACCTTTTGA CACCTTTGAG 
TACTTCCAGG ACAAGGAGGA CGAGCTGGAC AAGCCGTACA ATGACCGCTC CTACCTCAGC 
TCTGAGGGTT CGGCACGTGA CGATGCCCGC ACAGAATTCG TGGCCCTGCT GACGAGCGGC 
CCCGAGCCGT GGCACCCCAC ATCCAGCGCG GTGGCCAAGG CTCGCTTCAC CCTGCTGCGC 
TCCTACCTGC TCTTCTCCAT CAGCTACGAG CGGCTGGGCC GGCCGAGCCG TGTGCGTTTC 
AGTGACCCCG AGGGCACCGT GCTGTTCGAG CACCCAGTGC AGAAGAGCGC AGCCCCTGAG 
GACGGCATGC TCTGTGGGAT GTGGAGGACA GTGTCCAAAG CCAACATACA GCTGCTGCGT 
GCGGAGCAGC TCCGTGTGTC CCTCATCACC CGTGCGCAGC CCTCCGGAGA GGTGCATGGG 
CACATCCTGA AGCACCGGGC GCTCTTTGCA GAGACATTTG GTGCCATCCT GACCTCATCA 
GACCCCGCGC ACCTGGGGGC CGGGGGCATG GCCATGCTGA CGCTGAGCGA CACCGAGAAC 
AACCTACACT TCATCCTCAT GGCCCGTGGG CTGCTGGAGC CCGGTGCTGA GGAGTCCCCC 
TGGGTACCGC TGCGGGTCCG CATCCTGCAC CAGGGCCAGA CGCTGCGCGA GGCCCATGCC 
AACATCACTA TGGAGGACCC CGACTTCGCG GAGGTACTGA GCGACCTGTC TGCCCATGAG 
CTGCAGTGGT TGGCACAGGG GCAGCTCCGC ATCGTGGCTG ACACCCAGGG CCGGCACCCG 
CGCCAGCTGG AGGGCACCAT TACCGCCCGC CGCAGCTGTG ACACCATCCA AAGCGTGCTG 
TGCGGCGCAG ATGCATTGCA GCCCACCAAG ACGGGGGCGG TGGGCTCGGC CAAGCTGGCG 
CTGCATGAGA ACGGCACGCT GGAGTACCAG GTGCGGGTGG TGGGCACGGC CAGCGAGGTG 
GTGGGGGTCA CACTGGAGAC CAAACCGCGG CGGAAGAACA GGAGGAACGT CCTCTTCGAC 
ATGACACCCA GCTATAGGGC TGGGATGGCT CAAGGAGTGT GGCACAGCCC CAGCGCCCGC 
GATGTGCACA TGCTGCTGCA GAATGAGCTC TTCCTCAACG TGGCCACCAA GGACTGGGCA 
GAGGGAGAGC TGCGGGGCCA GGTCATCTCC CTGCCCTACA GTGGGCTCCT GGCACGCTAC 
ACAGAGATGC CGGTGCCGCT GGCAGGGCAG CTGGTATCCC CCCCAGTGAG CAGCGGGGCC 
GGGGGCCACG CGTGGCTGTC ACTGGATGAG CACTGCCACC TGCACTACGA GATAGTGGTG 
GCGGGTCTGG GCCGCCCGGC CGATGGCACA GTCAGTGCCC AGCTGCACGG CGTGGCCGAG 
CTGGGCGAGA TGGGCACTCG GCCGCACCTG CACAAGCGGA TGCTCAAGGG GTTCTATGGC 
ACTGAGGCTC AGGGGGTGGT GAAGGACCTG GATGCTGAGC TGCTGCAGCA CCTGGCACAG 
GGCACCGCAT TCCTGCAAGT CAGCACCAAA GCACACCCGC GTGGGGAGAT GCGGGGATGG 
GTGCACATCC CCAACCGCTG TCAGCCAGGA GGGGCCCGCC TGTCCACCGG GGAGGCTGAG 
CTCTCAGAGG GCCCTAAGGG CAGGAATGTG GAACAGCTGA AAAAGGACCC CAACTCCTGC 
TTCTTCGAGG GGCAGCACCA TGCACACGGC ACCCGCTGGG CGCCTGACTA CGACAAGAAG 
TGCTCCATCT GCAGCTGCCA GAAGCGCACG GTCATCTGCG ATCCCATCCT GTGCCAGCCC 
CTCAACTGTA CCCGCCAGGT GCACCCTGAG GAGCTGTGCT GCCCCATCTG TGAAGAGAAG 
AAGATGGAGC AGGAGGAGCT GAAGCTGGAA CGGGCACGGG ACAGCAGCGA GGGCTGCTAC 
TTTGATGGGG ACAAGACATG GCGAGGCTCT GGCACCCGCT GGCACCCTGT CGTGCCGCCG 
TTTGGCCTCA TCAAATGTGC CATCTGCACC TGCAAGGGCA CCACGGGTGA AGTGCACTGC 
GAGAAGGTGC AGTGTCCGCG CCTCACCTGT GCCAACCCTG TGCGCGTCAG CCCCTCCGAC 
TGCTGCAAGC AGTGCCCAGC CCCTGAGAAG AGCATCCCTG AGCTGACTGA TGGCATGCAG 
GCGGACGGAC CCCGTGCGTG CCGCTTTGGG CGCCGCTGGT ACCTCAACAA CGAGAGCTGG 
CACCCCTCAG TGCCACCCTT TGGGGAGATG AAGTGCATCC TGTGCTGGTG TGTGTCAGGA 
GAGACCCACT GCCAGCGCCA GGAGTGCCCG CCAGATGCCT GTGCCAGCCC CACCAGGAGG 
GACAACCCCT GCTGTGCCAA GTGTCGCGCT CCGGATGCAC CCTCAGAAAA GATGTACGAA 
GCCACAGCAG AGGCATGGAG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146860.1
CDS	19..3585
Translation	MPQFPPCQGRGSPSFPSFPGKGRELPLLLPHIPAAEPGDCDELRVRGASGGFLAPAGRPTAIARLLLPALPRSLPAVPPGLRHGGGHRAVTLARPRSPQPSPATGSQANRDNGREEQGQAFPWCLLPAHHRCCPHQPGHMWVKSRIRAGCCSHTLGRGTSLGPQVLSPRSWERSCGPALGLPSAPGFGDAKGTCCGGSQLPLAHAAAYKAGPGPAAGQRRWQRRQQAAPGPPSPPPAARFPRFPGTMRTALLLLALLALPVRTARPKLALPIRPDSEPLPPGGAAGCAFGGRFYALEETWHPDLGEPFGVMRCVICHCETQRNRRGKPVGKVNCKNMKQDCPVPTCPRATLLPGHCCHTCPKALPGAPEKSYKPPFDTFEYFQDKEDELDKPYNDRSYLSSEGSARDDARTEFVALLTSGPEPWHPTSSAVAKARFTLLRSYLLFSISYERLGRPSRVRFSDPEGTVLFEHPVQKSAAPEDGMLCGMWRTVSKANIQLLRAEQLRVSLITRAQPSGEVHGHILKHRALFAETFGAILTSSDPAHLGAGGMAMLTLSDTENNLHFILMARGLLEPGAEESPWVPLRVRILHQGQTLREAHANITMEDPDFAEVLSDLSAHELQWLAQGQLRIVADTQGRHPRQLEGTITARRSCDTIQSVLCGADALQPTKTGAVGSAKLALHENGTLEYQVRVVGTASEVVGVTLETKPRRKNRRNVLFDMTPSYRAGMAQGVWHSPSARDVHMLLQNELFLNVATKDWAEGELRGQVISLPYSGLLARYTEMPVPLAGQLVSPPVSSGAGGHAWLSLDEHCHLHYEIVVAGLGRPADGTVSAQLHGVAELGEMGTRPHLHKRMLKGFYGTEAQGVVKDLDAELLQHLAQGTAFLQVSTKAHPRGEMRGWVHIPNRCQPGGARLSTGEAELSEGPKGRNVEQLKKDPNSCFFEGQHHAHGTRWAPDYDKKCSICSCQKRTVICDPILCQPLNCTRQVHPEELCCPICEEKKMEQEELKLERARDSSEGCYFDGDKTWRGSGTRWHPVVPPFGLIKCAICTCKGTTGEVHCEKVQCPRLTCANPVRVSPSDCCKQCPAPEKSIPELTDGMQADGPRACRFGRRWYLNNESWHPSVPPFGEMKCILCWCVSGETHCQRQECPPDACASPTRRDNPCCAKCRAPDAPSEKMYEATAEAWSR

Target ORF information:

RefSeq Version	XM_015291374.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chordin (CHRD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291374.1

ORF Insert Sequence:

ATGCCTCAGT TTCCCCCATG CCAAGGCCGC GGCTCTCCCA GCTTTCCTTC CTTCCCGGGA 
AAGGGGAGGG AGCTTCCCCT GTTGCTTCCC CACATCCCCG CAGCCGAGCC AGGAGACTGT 
GACGAGCTCC GGGTGCGCGG AGCCAGCGGC GGATTCCTGG CACCGGCGGG GCGGCCGACA 
GCCATCGCCC GCCTCCTCCT CCCCGCTCTG CCCCGCTCGC TCCCCGCCGT TCCCCCCGGG 
CTGCGCCACG GTGGTGGGCA CCGGGCGGTG ACGCTGGCAC GGCCACGCAG CCCCCAGCCC 
TCCCCTGCCA CCGGATCCCA GGCCAACAGG GACAACGGGA GAGAGGAGCA GGGACAGGCC 
TTCCCATGGT GTCTGCTCCC TGCCCACCAC CGCTGCTGCC CTCATCAGCC CGGGCACATG 
TGGGTGAAGA GCAGGATCCG TGCTGGGTGC TGTTCCCATA CCCTCGGCCG CGGCACCTCC 
CTGGGACCCC AGGTCTTGTC CCCACGCTCG TGGGAACGGT CCTGTGGTCC TGCTCTGGGA 
TTGCCATCTG CACCAGGATT TGGGGATGCC AAAGGCACAT GTTGTGGGGG CTCGCAGCTC 
CCGCTTGCCC ATGCCGCCGC CTATAAAGCG GGGCCGGGGC CGGCGGCCGG GCAGAGGCGG 
TGGCAGCGGA GGCAGCAGGC AGCGCCCGGC CCCCCCTCCC CGCCCCCGGC CGCCCGGTTC 
CCCCGGTTCC CCGGCACCAT GCGCACCGCC CTGCTGCTGC TCGCCCTGCT CGCCCTGCCC 
GTCCGCACCG CCCGCCCCAA GCTCGCCCTG CCCATCCGGC CCGACAGCGA GCCGCTGCCC 
CCCGGGGGGG CGGCAGGTTG TGCCTTCGGG GGACGTTTCT ACGCGCTGGA GGAGACGTGG 
CACCCCGACC TGGGGGAGCC CTTTGGGGTG ATGCGCTGCG TGATCTGCCA CTGCGAGACG 
CAGAGGAATC GCCGAGGGAA ACCAGTGGGG AAAGTGAACT GCAAGAACAT GAAGCAGGAT 
TGCCCCGTTC CCACCTGCCC CCGTGCCACG CTGTTGCCCG GGCACTGCTG CCATACCTGT 
CCCAAAGCCT TGCCAGGAGC CCCGGAGAAG AGCTACAAGC CACCTTTTGA CACCTTTGAG 
TACTTCCAGG ACAAGGAGGA CGAGCTGGAC AAGCCGTACA ATGACCGCTC CTACCTCAGC 
TCTGAGGGTT CGGCACGTGA CGATGCCCGC ACAGAATTCG TGGCCCTGCT GACGAGCGGC 
CCCGAGCCGT GGCACCCCAC ATCCAGCGCG GTGGCCAAGG CTCGCTTCAC CCTGCTGCGC 
TCCTACCTGC TCTTCTCCAT CAGCTACGAG CGGCTGGGCC GGCCGAGCCG TGTGCGTTTC 
AGTGACCCCG AGGGCACCGT GCTGTTCGAG CACCCAGTGC AGAAGAGCGC AGCCCCTGAG 
GACGGCATGC TCTGTGGGAT GTGGAGGACA GTGTCCAAAG CCAACATACA GCTGCTGCGT 
GCGGAGCAGC TCCGTGTGTC CCTCATCACC CGTGCGCAGC CCTCCGGAGA GGTGCATGGG 
CACATCCTGA AGCACCGGGC GCTCTTTGCA GAGACATTTG GTGCCATCCT GACCTCATCA 
GACCCCGCGC ACCTGGGGGC CGGGGGCATG GCCATGCTGA CGCTGAGCGA CACCGAGAAC 
AACCTACACT TCATCCTCAT GGCCCGTGGG CTGCTGGAGC CCGGTGCTGA GGAGTCCCCC 
TGGGTACCGC TGCGGGTCCG CATCCTGCAC CAGGGCCAGA CGCTGCGCGA GGCCCATGCC 
AACATCACTA TGGAGGACCC CGACTTCGCG GAGGTACTGA GCGACCTGTC TGCCCATGAG 
CTGCAGTGGT TGGCACAGGG GCAGCTCCGC ATCGTGGCTG ACACCCAGGG CCGGCACCCG 
CGCCAGCTGG AGGGCACCAT TACCGCCCGC CGCAGCTGTG ACACCATCCA AAGCGTGCTG 
TGCGGCGCAG ATGCATTGCA GCCCACCAAG ACGGGGGCGG TGGGCTCGGC CAAGCTGGCG 
CTGCATGAGA ACGGCACGCT GGAGTACCAG GTGCGGGTGG TGGGCACGGC CAGCGAGGTG 
GTGGGGGTCA CACTGGAGAC CAAACCGCGG CGGAAGAACA GGAGGAACGT CCTCTTCGAC 
ATGACACCCA GCTATAGGGC TGGGATGGCT CAAGGAGTGT GGCACAGCCC CAGCGCCCGC 
GATGTGCACA TGCTGCTGCA GAATGAGCTC TTCCTCAACG TGGCCACCAA GGACTGGGCA 
GAGGGAGAGC TGCGGGGCCA GGTCATCTCC CTGCCCTACA GTGGGCTCCT GGCACGCTAC 
ACAGAGATGC CGGTGCCGCT GGCAGGGCAG CTGGTATCCC CCCCAGTGAG CAGCGGGGCC 
GGGGGCCACG CGTGGCTGTC ACTGGATGAG CACTGCCACC TGCACTACGA GATAGTGGTG 
GCGGGTCTGG GCCGCCCGGC CGATGGCACA GTCAGTGCCC AGCTGCACGG CGTGGCCGAG 
CTGGGCGAGA TGGGCACTCG GCCGCACCTG CACAAGCGGA TGCTCAAGGG GTTCTATGGC 
ACTGAGGCTC AGGGGGTGGT GAAGGACCTG GATGCTGAGC TGCTGCAGCA CCTGGCACAG 
GGCACCGCAT TCCTGCAAGT CAGCACCAAA GCACACCCGC GTGGGGAGAT GCGGGGATGG 
GTGCACATCC CCAACCGCTG TCAGCCAGGA GGGGCCCGCC TGTCCACCGG GGAGGCTGAG 
CTCTCAGAGG GCCCTAAGGG CAGGAATGTG GAACAGCTGA AAAAGGACCC CAACTCCTGC 
TTCTTCGAGG GGCAGCACCA TGCACACGGC ACCCGCTGGG CGCCTGACTA CGACAAGAAG 
TGCTCCATCT GCAGCTGCCA GAAGCGCACG GTCATCTGCG ATCCCATCCT GTGCCAGCCC 
CTCAACTGTA CCCGCCAGGT GCACCCTGAG GAGCTGTGCT GCCCCATCTG TGAAGAGAAG 
AAGATGGAGC AGGAGGAGCT GAAGCTGGAA CGGGCACGGG ACAGCAGCGA GGGCTGCTAC 
TTTGATGGGG ACAAGACATG GCGAGGCTCT GGCACCCGCT GGCACCCTGT CGTGCCGCCG 
TTTGGCCTCA TCAAATGTGC CATCTGCACC TGCAAGGGCA CCACGGGTGA AGTGCACTGC 
GAGAAGGTGC AGTGTCCGCG CCTCACCTGT GCCAACCCTG TGCGCGTCAG CCCCTCCGAC 
TGCTGCAAGC AGTGCCCAGC CCCTGAGAAG AGCATCCCTG AGCTGACTGA TGGCATGCAG 
GCGGACGGAC CCCGTGCGTG CCGCTTTGGG CGCCGCTGGT ACCTCAACAA CGAGAGCTGG 
CACCCCTCAG TGCCACCCTT TGGGGAGATG AAGTGCATCC TGTGCTGGTG TGTGTCAGGA 
GAGACCCACT GCCAGCGCCA GGAGTGCCCG CCAGATGCCT GTGCCAGCCC CACCAGGAGG 
GACAACCCCT GCTGTGCCAA GTGTCGCGCT CCGGATGCAC CCTCAGAAAA GATGTACGAA 
GCCACAGCAG AGGCATGGAG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRD ( XM_015291374.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015146860.1 Gallus gallus chordin (CHRD), transcript variant X3, mRNA.

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CloneID	OGa39872
Clone ID Related Accession (Same CDS sequence)	XM_015291374.1 , XM_015291374.2
Accession Version	XM_015291374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	chordin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGT TTCCCCCATG CCAAGGCCGC GGCTCTCCCA GCTTTCCTTC CTTCCCGGGA 
AAGGGGAGGG AGCTTCCCCT GTTGCTTCCC CACATCCCCG CAGCCGAGCC AGGAGACTGT 
GACGAGCTCC GGGTGCGCGG AGCCAGCGGC GGATTCCTGG CACCGGCGGG GCGGCCGACA 
GCCATCGCCC GCCTCCTCCT CCCCGCTCTG CCCCGCTCGC TCCCCGCCGT TCCCCCCGGG 
CTGCGCCACG GTGGTGGGCA CCGGGCGGTG ACGCTGGCAC GGCCACGCAG CCCCCAGCCC 
TCCCCTGCCA CCGGATCCCA GGCCAACAGG GACAACGGGA GAGAGGAGCA GGGACAGGCC 
TTCCCATGGT GTCTGCTCCC TGCCCACCAC CGCTGCTGCC CTCATCAGCC CGGGCACATG 
TGGGTGAAGA GCAGGATCCG TGCTGGGTGC TGTTCCCATA CCCTCGGCCG CGGCACCTCC 
CTGGGACCCC AGGTCTTGTC CCCACGCTCG TGGGAACGGT CCTGTGGTCC TGCTCTGGGA 
TTGCCATCTG CACCAGGATT TGGGGATGCC AAAGGCACAT GTTGTGGGGG CTCGCAGCTC 
CCGCTTGCCC ATGCCGCCGC CTATAAAGCG GGGCCGGGGC CGGCGGCCGG GCAGAGGCGG 
TGGCAGCGGA GGCAGCAGGC AGCGCCCGGC CCCCCCTCCC CGCCCCCGGC CGCCCGGTTC 
CCCCGGTTCC CCGGCACCAT GCGCACCGCC CTGCTGCTGC TCGCCCTGCT CGCCCTGCCC 
GTCCGCACCG CCCGCCCCAA GCTCGCCCTG CCCATCCGGC CCGACAGCGA GCCGCTGCCC 
CCCGGGGGGG CGGCAGGTTG TGCCTTCGGG GGACGTTTCT ACGCGCTGGA GGAGACGTGG 
CACCCCGACC TGGGGGAGCC CTTTGGGGTG ATGCGCTGCG TGATCTGCCA CTGCGAGACG 
CAGAGGAATC GCCGAGGGAA ACCAGTGGGG AAAGTGAACT GCAAGAACAT GAAGCAGGAT 
TGCCCCGTTC CCACCTGCCC CCGTGCCACG CTGTTGCCCG GGCACTGCTG CCATACCTGT 
CCCAAAGCCT TGCCAGGAGC CCCGGAGAAG AGCTACAAGC CACCTTTTGA CACCTTTGAG 
TACTTCCAGG ACAAGGAGGA CGAGCTGGAC AAGCCGTACA ATGACCGCTC CTACCTCAGC 
TCTGAGGGTT CGGCACGTGA CGATGCCCGC ACAGAATTCG TGGCCCTGCT GACGAGCGGC 
CCCGAGCCGT GGCACCCCAC ATCCAGCGCG GTGGCCAAGG CTCGCTTCAC CCTGCTGCGC 
TCCTACCTGC TCTTCTCCAT CAGCTACGAG CGGCTGGGCC GGCCGAGCCG TGTGCGTTTC 
AGTGACCCCG AGGGCACCGT GCTGTTCGAG CACCCAGTGC AGAAGAGCGC AGCCCCTGAG 
GACGGCATGC TCTGTGGGAT GTGGAGGACA GTGTCCAAAG CCAACATACA GCTGCTGCGT 
GCGGAGCAGC TCCGTGTGTC CCTCATCACC CGTGCGCAGC CCTCCGGAGA GGTGCATGGG 
CACATCCTGA AGCACCGGGC GCTCTTTGCA GAGACATTTG GTGCCATCCT GACCTCATCA 
GACCCCGCGC ACCTGGGGGC CGGGGGCATG GCCATGCTGA CGCTGAGCGA CACCGAGAAC 
AACCTACACT TCATCCTCAT GGCCCGTGGG CTGCTGGAGC CCGGTGCTGA GGAGTCCCCC 
TGGGTACCGC TGCGGGTCCG CATCCTGCAC CAGGGCCAGA CGCTGCGCGA GGCCCATGCC 
AACATCACTA TGGAGGACCC CGACTTCGCG GAGGTACTGA GCGACCTGTC TGCCCATGAG 
CTGCAGTGGT TGGCACAGGG GCAGCTCCGC ATCGTGGCTG ACACCCAGGG CCGGCACCCG 
CGCCAGCTGG AGGGCACCAT TACCGCCCGC CGCAGCTGTG ACACCATCCA AAGCGTGCTG 
TGCGGCGCAG ATGCATTGCA GCCCACCAAG ACGGGGGCGG TGGGCTCGGC CAAGCTGGCG 
CTGCATGAGA ACGGCACGCT GGAGTACCAG GTGCGGGTGG TGGGCACGGC CAGCGAGGTG 
GTGGGGGTCA CACTGGAGAC CAAACCGCGG CGGAAGAACA GGAGGAACGT CCTCTTCGAC 
ATGACACCCA GCTATAGGGC TGGGATGGCT CAAGGAGTGT GGCACAGCCC CAGCGCCCGC 
GATGTGCACA TGCTGCTGCA GAATGAGCTC TTCCTCAACG TGGCCACCAA GGACTGGGCA 
GAGGGAGAGC TGCGGGGCCA GGTCATCTCC CTGCCCTACA GTGGGCTCCT GGCACGCTAC 
ACAGAGATGC CGGTGCCGCT GGCAGGGCAG CTGGTATCCC CCCCAGTGAG CAGCGGGGCC 
GGGGGCCACG CGTGGCTGTC ACTGGATGAG CACTGCCACC TGCACTACGA GATAGTGGTG 
GCGGGTCTGG GCCGCCCGGC CGATGGCACA GTCAGTGCCC AGCTGCACGG CGTGGCCGAG 
CTGGGCGAGA TGGGCACTCG GCCGCACCTG CACAAGCGGA TGCTCAAGGG GTTCTATGGC 
ACTGAGGCTC AGGGGGTGGT GAAGGACCTG GATGCTGAGC TGCTGCAGCA CCTGGCACAG 
GGCACCGCAT TCCTGCAAGT CAGCACCAAA GCACACCCGC GTGGGGAGAT GCGGGGATGG 
GTGCACATCC CCAACCGCTG TCAGCCAGGA GGGGCCCGCC TGTCCACCGG GGAGGCTGAG 
CTCTCAGAGG GCCCTAAGGG CAGGAATGTG GAACAGCTGA AAAAGGACCC CAACTCCTGC 
TTCTTCGAGG GGCAGCACCA TGCACACGGC ACCCGCTGGG CGCCTGACTA CGACAAGAAG 
TGCTCCATCT GCAGCTGCCA GAAGCGCACG GTCATCTGCG ATCCCATCCT GTGCCAGCCC 
CTCAACTGTA CCCGCCAGGT GCACCCTGAG GAGCTGTGCT GCCCCATCTG TGAAGAGAAG 
AAGATGGAGC AGGAGGAGCT GAAGCTGGAA CGGGCACGGG ACAGCAGCGA GGGCTGCTAC 
TTTGATGGGG ACAAGACATG GCGAGGCTCT GGCACCCGCT GGCACCCTGT CGTGCCGCCG 
TTTGGCCTCA TCAAATGTGC CATCTGCACC TGCAAGGGCA CCACGGGTGA AGTGCACTGC 
GAGAAGGTGC AGTGTCCGCG CCTCACCTGT GCCAACCCTG TGCGCGTCAG CCCCTCCGAC 
TGCTGCAAGC AGTGCCCAGC CCCTGAGAAG AGCATCCCTG AGCTGACTGA TGGCATGCAG 
GCGGACGGAC CCCGTGCGTG CCGCTTTGGG CGCCGCTGGT ACCTCAACAA CGAGAGCTGG 
CACCCCTCAG TGCCACCCTT TGGGGAGATG AAGTGCATCC TGTGCTGGTG TGTGTCAGGA 
GAGACCCACT GCCAGCGCCA GGAGTGCCCG CCAGATGCCT GTGCCAGCCC CACCAGGAGG 
GACAACCCCT GCTGTGCCAA GTGTCGCGCT CCGGATGCAC CCTCAGAAAA GATGTACGAA 
GCCACAGCAG AGGCATGGAG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015146860.1
CDS	35..3601
Translation	MPQFPPCQGRGSPSFPSFPGKGRELPLLLPHIPAAEPGDCDELRVRGASGGFLAPAGRPTAIARLLLPALPRSLPAVPPGLRHGGGHRAVTLARPRSPQPSPATGSQANRDNGREEQGQAFPWCLLPAHHRCCPHQPGHMWVKSRIRAGCCSHTLGRGTSLGPQVLSPRSWERSCGPALGLPSAPGFGDAKGTCCGGSQLPLAHAAAYKAGPGPAAGQRRWQRRQQAAPGPPSPPPAARFPRFPGTMRTALLLLALLALPVRTARPKLALPIRPDSEPLPPGGAAGCAFGGRFYALEETWHPDLGEPFGVMRCVICHCETQRNRRGKPVGKVNCKNMKQDCPVPTCPRATLLPGHCCHTCPKALPGAPEKSYKPPFDTFEYFQDKEDELDKPYNDRSYLSSEGSARDDARTEFVALLTSGPEPWHPTSSAVAKARFTLLRSYLLFSISYERLGRPSRVRFSDPEGTVLFEHPVQKSAAPEDGMLCGMWRTVSKANIQLLRAEQLRVSLITRAQPSGEVHGHILKHRALFAETFGAILTSSDPAHLGAGGMAMLTLSDTENNLHFILMARGLLEPGAEESPWVPLRVRILHQGQTLREAHANITMEDPDFAEVLSDLSAHELQWLAQGQLRIVADTQGRHPRQLEGTITARRSCDTIQSVLCGADALQPTKTGAVGSAKLALHENGTLEYQVRVVGTASEVVGVTLETKPRRKNRRNVLFDMTPSYRAGMAQGVWHSPSARDVHMLLQNELFLNVATKDWAEGELRGQVISLPYSGLLARYTEMPVPLAGQLVSPPVSSGAGGHAWLSLDEHCHLHYEIVVAGLGRPADGTVSAQLHGVAELGEMGTRPHLHKRMLKGFYGTEAQGVVKDLDAELLQHLAQGTAFLQVSTKAHPRGEMRGWVHIPNRCQPGGARLSTGEAELSEGPKGRNVEQLKKDPNSCFFEGQHHAHGTRWAPDYDKKCSICSCQKRTVICDPILCQPLNCTRQVHPEELCCPICEEKKMEQEELKLERARDSSEGCYFDGDKTWRGSGTRWHPVVPPFGLIKCAICTCKGTTGEVHCEKVQCPRLTCANPVRVSPSDCCKQCPAPEKSIPELTDGMQADGPRACRFGRRWYLNNESWHPSVPPFGEMKCILCWCVSGETHCQRQECPPDACASPTRRDNPCCAKCRAPDAPSEKMYEATAEAWSR

Target ORF information:

RefSeq Version	XM_015291374.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus chordin (CHRD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015291374.2

ORF Insert Sequence:

ATGCCTCAGT TTCCCCCATG CCAAGGCCGC GGCTCTCCCA GCTTTCCTTC CTTCCCGGGA 
AAGGGGAGGG AGCTTCCCCT GTTGCTTCCC CACATCCCCG CAGCCGAGCC AGGAGACTGT 
GACGAGCTCC GGGTGCGCGG AGCCAGCGGC GGATTCCTGG CACCGGCGGG GCGGCCGACA 
GCCATCGCCC GCCTCCTCCT CCCCGCTCTG CCCCGCTCGC TCCCCGCCGT TCCCCCCGGG 
CTGCGCCACG GTGGTGGGCA CCGGGCGGTG ACGCTGGCAC GGCCACGCAG CCCCCAGCCC 
TCCCCTGCCA CCGGATCCCA GGCCAACAGG GACAACGGGA GAGAGGAGCA GGGACAGGCC 
TTCCCATGGT GTCTGCTCCC TGCCCACCAC CGCTGCTGCC CTCATCAGCC CGGGCACATG 
TGGGTGAAGA GCAGGATCCG TGCTGGGTGC TGTTCCCATA CCCTCGGCCG CGGCACCTCC 
CTGGGACCCC AGGTCTTGTC CCCACGCTCG TGGGAACGGT CCTGTGGTCC TGCTCTGGGA 
TTGCCATCTG CACCAGGATT TGGGGATGCC AAAGGCACAT GTTGTGGGGG CTCGCAGCTC 
CCGCTTGCCC ATGCCGCCGC CTATAAAGCG GGGCCGGGGC CGGCGGCCGG GCAGAGGCGG 
TGGCAGCGGA GGCAGCAGGC AGCGCCCGGC CCCCCCTCCC CGCCCCCGGC CGCCCGGTTC 
CCCCGGTTCC CCGGCACCAT GCGCACCGCC CTGCTGCTGC TCGCCCTGCT CGCCCTGCCC 
GTCCGCACCG CCCGCCCCAA GCTCGCCCTG CCCATCCGGC CCGACAGCGA GCCGCTGCCC 
CCCGGGGGGG CGGCAGGTTG TGCCTTCGGG GGACGTTTCT ACGCGCTGGA GGAGACGTGG 
CACCCCGACC TGGGGGAGCC CTTTGGGGTG ATGCGCTGCG TGATCTGCCA CTGCGAGACG 
CAGAGGAATC GCCGAGGGAA ACCAGTGGGG AAAGTGAACT GCAAGAACAT GAAGCAGGAT 
TGCCCCGTTC CCACCTGCCC CCGTGCCACG CTGTTGCCCG GGCACTGCTG CCATACCTGT 
CCCAAAGCCT TGCCAGGAGC CCCGGAGAAG AGCTACAAGC CACCTTTTGA CACCTTTGAG 
TACTTCCAGG ACAAGGAGGA CGAGCTGGAC AAGCCGTACA ATGACCGCTC CTACCTCAGC 
TCTGAGGGTT CGGCACGTGA CGATGCCCGC ACAGAATTCG TGGCCCTGCT GACGAGCGGC 
CCCGAGCCGT GGCACCCCAC ATCCAGCGCG GTGGCCAAGG CTCGCTTCAC CCTGCTGCGC 
TCCTACCTGC TCTTCTCCAT CAGCTACGAG CGGCTGGGCC GGCCGAGCCG TGTGCGTTTC 
AGTGACCCCG AGGGCACCGT GCTGTTCGAG CACCCAGTGC AGAAGAGCGC AGCCCCTGAG 
GACGGCATGC TCTGTGGGAT GTGGAGGACA GTGTCCAAAG CCAACATACA GCTGCTGCGT 
GCGGAGCAGC TCCGTGTGTC CCTCATCACC CGTGCGCAGC CCTCCGGAGA GGTGCATGGG 
CACATCCTGA AGCACCGGGC GCTCTTTGCA GAGACATTTG GTGCCATCCT GACCTCATCA 
GACCCCGCGC ACCTGGGGGC CGGGGGCATG GCCATGCTGA CGCTGAGCGA CACCGAGAAC 
AACCTACACT TCATCCTCAT GGCCCGTGGG CTGCTGGAGC CCGGTGCTGA GGAGTCCCCC 
TGGGTACCGC TGCGGGTCCG CATCCTGCAC CAGGGCCAGA CGCTGCGCGA GGCCCATGCC 
AACATCACTA TGGAGGACCC CGACTTCGCG GAGGTACTGA GCGACCTGTC TGCCCATGAG 
CTGCAGTGGT TGGCACAGGG GCAGCTCCGC ATCGTGGCTG ACACCCAGGG CCGGCACCCG 
CGCCAGCTGG AGGGCACCAT TACCGCCCGC CGCAGCTGTG ACACCATCCA AAGCGTGCTG 
TGCGGCGCAG ATGCATTGCA GCCCACCAAG ACGGGGGCGG TGGGCTCGGC CAAGCTGGCG 
CTGCATGAGA ACGGCACGCT GGAGTACCAG GTGCGGGTGG TGGGCACGGC CAGCGAGGTG 
GTGGGGGTCA CACTGGAGAC CAAACCGCGG CGGAAGAACA GGAGGAACGT CCTCTTCGAC 
ATGACACCCA GCTATAGGGC TGGGATGGCT CAAGGAGTGT GGCACAGCCC CAGCGCCCGC 
GATGTGCACA TGCTGCTGCA GAATGAGCTC TTCCTCAACG TGGCCACCAA GGACTGGGCA 
GAGGGAGAGC TGCGGGGCCA GGTCATCTCC CTGCCCTACA GTGGGCTCCT GGCACGCTAC 
ACAGAGATGC CGGTGCCGCT GGCAGGGCAG CTGGTATCCC CCCCAGTGAG CAGCGGGGCC 
GGGGGCCACG CGTGGCTGTC ACTGGATGAG CACTGCCACC TGCACTACGA GATAGTGGTG 
GCGGGTCTGG GCCGCCCGGC CGATGGCACA GTCAGTGCCC AGCTGCACGG CGTGGCCGAG 
CTGGGCGAGA TGGGCACTCG GCCGCACCTG CACAAGCGGA TGCTCAAGGG GTTCTATGGC 
ACTGAGGCTC AGGGGGTGGT GAAGGACCTG GATGCTGAGC TGCTGCAGCA CCTGGCACAG 
GGCACCGCAT TCCTGCAAGT CAGCACCAAA GCACACCCGC GTGGGGAGAT GCGGGGATGG 
GTGCACATCC CCAACCGCTG TCAGCCAGGA GGGGCCCGCC TGTCCACCGG GGAGGCTGAG 
CTCTCAGAGG GCCCTAAGGG CAGGAATGTG GAACAGCTGA AAAAGGACCC CAACTCCTGC 
TTCTTCGAGG GGCAGCACCA TGCACACGGC ACCCGCTGGG CGCCTGACTA CGACAAGAAG 
TGCTCCATCT GCAGCTGCCA GAAGCGCACG GTCATCTGCG ATCCCATCCT GTGCCAGCCC 
CTCAACTGTA CCCGCCAGGT GCACCCTGAG GAGCTGTGCT GCCCCATCTG TGAAGAGAAG 
AAGATGGAGC AGGAGGAGCT GAAGCTGGAA CGGGCACGGG ACAGCAGCGA GGGCTGCTAC 
TTTGATGGGG ACAAGACATG GCGAGGCTCT GGCACCCGCT GGCACCCTGT CGTGCCGCCG 
TTTGGCCTCA TCAAATGTGC CATCTGCACC TGCAAGGGCA CCACGGGTGA AGTGCACTGC 
GAGAAGGTGC AGTGTCCGCG CCTCACCTGT GCCAACCCTG TGCGCGTCAG CCCCTCCGAC 
TGCTGCAAGC AGTGCCCAGC CCCTGAGAAG AGCATCCCTG AGCTGACTGA TGGCATGCAG 
GCGGACGGAC CCCGTGCGTG CCGCTTTGGG CGCCGCTGGT ACCTCAACAA CGAGAGCTGG 
CACCCCTCAG TGCCACCCTT TGGGGAGATG AAGTGCATCC TGTGCTGGTG TGTGTCAGGA 
GAGACCCACT GCCAGCGCCA GGAGTGCCCG CCAGATGCCT GTGCCAGCCC CACCAGGAGG 
GACAACCCCT GCTGTGCCAA GTGTCGCGCT CCGGATGCAC CCTCAGAAAA GATGTACGAA 
GCCACAGCAG AGGCATGGAG CCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chordin regulates primitive streak development and the stability of induced neural cells, but is not sufficient for neural induction in the chick embryo.
Development (Cambridge, England)125(3)507-19(1998 Feb)
Streit A,Lee KJ,Woo I,Roberts C,Jessell TM,Stern CD

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CHRD cDNA ORF clone, Gallus gallus(chicken)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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