focad cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	focad
Entrez Gene ID	549009
Full Name	focadhesin
Synonyms	kiaa1797
General protein information	Preferred Names focadhesin Names focadhesin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012962109.2	XP_012817563.1	focadhesin isoform X2
XM_031899765.1	XP_031755625.1	focadhesin isoform X2
NM_001374673.1	NP_001361602.1	focadhesin
XM_012962109.3	XP_012817563.1	focadhesin isoform X1
XM_012962106.2	XP_012817560.1	focadhesin isoform X1
NM_001016255.2	NP_001016255.2	focadhesin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following focad gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the focad cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa20837	NM_001016255.2 Latest version!	Xenopus tropicalis focadhesin (focad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OXa27621	NM_001374673.1 Latest version!	Xenopus tropicalis focadhesin (focad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa27622	XM_012962109.3 Latest version!	Xenopus tropicalis focadhesin (focad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa47895	XM_031899765.1 Latest version!	Xenopus tropicalis focadhesin (focad), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa27621	XM_012962106.2 Latest version!	Xenopus tropicalis focadhesin (focad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OXa27622	XM_012962109.2	Xenopus tropicalis focadhesin (focad), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

focad ( NM_001016255.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001016255.2 Xenopus tropicalis focadhesin (focad), mRNA.

Price & Availability↑

CloneID	OXa20837
Clone ID Related Accession (Same CDS sequence)	NM_001016255.2
Accession Version	NM_001016255.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	focadhesin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR761738.2. On Oct 14, 2005 this sequence version replaced NM_001016255.1. ##Evidence-Data-START## Transcript exon combination :: CR761738.2, BC171264.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028291, SAMD00028313 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACGC CATTCACTGA AACTAGTGTG CAGCATGCAT GTTTCCCCAA GATGTCAGAA 
GATCTCAAGA AGAGATTAGA ATTTCCAAAC TCATTAATTC AGACACAGGC GGTGACTCAG 
CTCATTGCAT CTGTGTTAAA AGAAAATGTC TCATTGGGGA AGATAAAACA GTCTTCAAAT 
CAGACCCCAG CTCTGAATAT TCTTTGGGAG AAGTGCTGCA GTAATAATGT GGTTGTAAGG 
ACAGCATGTT GTGAAGCATT GGTGCTACTG GTTGAACAGG ATCATGCTGA TTTCGATTAT 
GTTCTTAATG GAATTTTGAA CCTTATACCT TCTGCTGGGA CTGTACAAGG ACTGCTGAAA 
TGTGTATGGC GCCTCCTATA TACTCAAGCA AGGAATGCAG AAAAGGATGG GGAACACAAA 
GATTTGGGCA TTTATAAAAT TTGGGTGCCA CAGCCATGTG ACCTGTGCTC TGGTGAACTT 
CGGTCACACT TTACTGCTGA GCTGCAAGTT GGAGTGACAT CGCCCCCCTC CCAGCAGCAG 
AACAATGGAG GGGGTGCGGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001016255.2
CDS	62..625
Translation	MATPFTETSVQHACFPKMSEDLKKRLEFPNSLIQTQAVTQLIASVLKENVSLGKIKQSSNQTPALNILWEKCCSNNVVVRTACCEALVLLVEQDHADFDYVLNGILNLIPSAGTVQGLLKCVWRLLYTQARNAEKDGEHKDLGIYKIWVPQPCDLCSGELRSHFTAELQVGVTSPPSQQQNNGGGAG

Target ORF information:

RefSeq Version	NM_001016255.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis focadhesin (focad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001016255.2

ORF Insert Sequence:

ATGGCCACGC CATTCACTGA AACTAGTGTG CAGCATGCAT GTTTCCCCAA GATGTCAGAA 
GATCTCAAGA AGAGATTAGA ATTTCCAAAC TCATTAATTC AGACACAGGC GGTGACTCAG 
CTCATTGCAT CTGTGTTAAA AGAAAATGTC TCATTGGGGA AGATAAAACA GTCTTCAAAT 
CAGACCCCAG CTCTGAATAT TCTTTGGGAG AAGTGCTGCA GTAATAATGT GGTTGTAAGG 
ACAGCATGTT GTGAAGCATT GGTGCTACTG GTTGAACAGG ATCATGCTGA TTTCGATTAT 
GTTCTTAATG GAATTTTGAA CCTTATACCT TCTGCTGGGA CTGTACAAGG ACTGCTGAAA 
TGTGTATGGC GCCTCCTATA TACTCAAGCA AGGAATGCAG AAAAGGATGG GGAACACAAA 
GATTTGGGCA TTTATAAAAT TTGGGTGCCA CAGCCATGTG ACCTGTGCTC TGGTGAACTT 
CGGTCACACT TTACTGCTGA GCTGCAAGTT GGAGTGACAT CGCCCCCCTC CCAGCAGCAG 
AACAATGGAG GGGGTGCGGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

focad ( NM_001374673.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001361602.1 Xenopus tropicalis focadhesin (focad), mRNA.

Price & Availability↑

CloneID	OXa27621
Clone ID Related Accession (Same CDS sequence)	NM_001374673.1 , XM_012962106.2
Accession Version	NM_001374673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	focadhesin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03003076.1, AAMC03003077.1 and AAMC03003078.1. On Oct 8, 2019 this sequence version replaced XM_012962106.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1696418, SAMN02230355 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACGC CATTCACTGA AACTAGTGTG CAGCATGCAT GTTTCCCCAA GATGTCAGAA 
GATCTCAAGA AGAGATTAGA ATTTCCAAAC TCATTAATTC AGACACAGGC GGTGACTCAG 
CTCATTGCAT CTGTGTTAAA AGAAAATGTC TCATTGGGGA AGATAAAACA GTCTTCAAAT 
CAGACCCCAG CTCTGAATAT TCTTTGGGAG AAGTGCTGCA GTAATAATGT GGTTGTAAGG 
ACAGCATGTT GTGAAGCATT GGTGCTACTG GTTGAACAGG ATCATGCTGA TTTCGATTAT 
GTTCTTAATG GAATTTTGAA CCTTATACCT TCTGCTGGGA CTGTACAAGG ACTGCTGAAA 
TGTGTATGGC GCCTCCTATA TACTCAAGCA AGGAATGCAG AAAAGGATGG GGAACACAAA 
GATTTGGGCA TTTATAAAAT TTGGAACCCC CCTCATCCAT TCATCAGCAT ACTGGAAAAC 
AGACCAGACT GTTGGCCTGC ACTTCTGCAG CAGATTTCTA CTTTGCCTCA GCTGTGTCAT 
GAAAGCTGTC CTTCAAGCAA TGTTTATTTT ATGGCGTCGT TTTTGAGGTA CTTGTATTGT 
GAACCCTCCC AGCTACAAGA GTACTCTGCC CTTCGAATAG GTCTCAGAAA AGCTTTAGTT 
ATGGTATCGG GGAAGAGTGG GTCCAACAAT CCGTCAAAAC CAGAAGAGCA CATTCTTCGG 
ATTTTCATTG ACATGATGGG CTACATTCAG TTTTTGGATT CAACTCAAGT TACTGAAGCA 
ACTTTGTACA TAAAAGATTT ATGCAGAAGC CTCCACCTGC ATCCAGATTT CTGGAGAAAT 
GAGCTCTCTG CAGTTTGCTT GCAGGCCTTA TGTGCATGTG AGGCAAGCTT AAAAATTGCA 
GGAGACTGCT CTACACTAAT AGAAATATTG GAACAAAATT CAGATCTGCT TGGTGAGAGC 
TTTCCCACAG AACAGGTATT AATTGGACTC AGTTTACTAT TGCTACAATC ACCAGCTAGC 
CAGCAAAAAC TAATCTTAAA GTTGGTAATG AAGATTCTGT CATTCAGAGG GGAAAACAAT 
TTTCCATCAA TCGTCCAAGT GTTAATTATG CCACTCCTCC AAATTTTATC TACCTCAGCG 
CTGGGATATT GTTTCTCTGA TGATGGTGGA AGCTCGAGAG AGCAGCTGGC TACTAGTCTT 
CTGGAAATGG TTCAGAATGC TGACTGTAGT GGGGAAAGGA GACTGAATGA TGTATCACTT 
CCTCTTCCTG TAACAACATG GCATTGTTCA ATGGCTGCTA CATTGAACAT ACTTAACAAG 
TTTCAAGATG ACCATTCTGC AGCACGTGAC TGGCTGTCTT CCATATCTTC GATGCTGGCC 
CCCAATACTC AAGTTCCTGT CAATGTTTCA CTTTTGCTAA GTTTCCTTCT GATTAGAGAA 
TCTGATTATG GCTTGCGTAA TGTTTTGAAA GTCATCACTG ATGTCTCCAA AGCTGATTCA 
TCTCAGGTTC CATATTTAGT TCCAGTTCTA ATGTTGAGAC TTGGACATTC CTTGGCACCA 
GAATCCTCCA GAGACATTCT GTACACTTTG CCTACCCTGG GCACCCACAA GGTATGTGTG 
GCTCAGATCT TGCGAGTCTT CCAAATGTTA GGAAGTACTT CAAAGCTTCA ACCCATTGCG 
CTTCGTCTTA TGACATCTTT GTGGGAGAAA CAGGACCGAA TCTATCCAGA ATTACAGAAG 
ATGATGGCCT TATGTGACAC TCAGTCATTA GCCACTGGGA AAGACACATT GTGGGAAAAG 
ATGTTGGCAA AAGCAGCAAC TGTCAGAGAT ATCTGCAGAC AGAGGCCATA TCAGCATGGG 
GCAGATATGC TGGCTGCTAT AACGCAAGTT TTACATGAAT GCACAAAAGC CGACCAAGCT 
ACTCCGGCTG CTTTAGCATT GCAGGGTCTT CATGCTCTCT GTCAAGCAGA GGTTGTAGAC 
ATTCGCTCTG CCTGGAATGC TCTTTCCTCC AAACTTAGTT GTGAAACACG TCCCCTTGTC 
CTCAAAGTGT TACATGAACT TTTTGCATTG GTCCCTTCTT TGACTGTTGA TACAGAAGAA 
TATCAGAAAT TTAAAACTGA GATTATCGGT ATCCTTTGGA GGCACACTCA AAGTAAGGAT 
CTCAATGTGA TGGTTTCTGC ATACAAATCT TTATGTGAGT TTGAGGCTGA TGAGCACTCC 
ATTCTTCATA TTCCTGAGCA GGCAAGGCCT GAGGTGGCAC CTAAAGATGA ATCTGACCTG 
GCTAGTGAAG AAGAGGAGAA AGAAACTGAC ATTTCTATCC CTGGCTCATC ATATATAAAA 
TTAATGGCTT TGACACCGGT ATCTGTTTTA CCAGCTCTTG AAGCTTTCCT TTCATCTCTT 
GTAAAGCAAG AAATGAGCCG CATGCCACGT GGTATTTATC ATACAGCTCT GCGTGGTGGT 
AATATTAGAT CTGACCAAGG CAAAACTATT GCAGGAGTCC CAAACTTCAT GCTAAAAATG 
TACGAGAAGA ATAAACAGCC TGGCCTGAAA CCTGGCCTTG CAGCTGGCAT GCTGCTGTGT 
TACGATCTCC CAATCCAGAC AGACAAGGAT GGCAGGCCCA TTAATCGTTT CCTTGCCAGT 
AGAGGGCGCA GCTACCAGCA GATGTTAACC GCACTTATCA ATGAGGTGAA TATTCAGTCT 
TCAGAATGGC ATCGCTCTAT ATTGCTGCCC CAGTCATGGC TTGGCTTCAT GACAAGGACA 
TACCATGCTG TTCTGCAAGG AAGGCAAGCT GAACTGGAAA TGCAGCTGAA ACATGGAAAG 
GATGACCCAC AAGAGCTTCA GTACAAAACA TTTACAGCTT GGTTGTGGGT AAGGGATGCA 
TTGACCGATG TCATTAGGTG TGCTGTGAAA GACAGTCCTG TGGTGAAGGG AAATTCTCTG 
TTGGCATTGA CTGGACTTGC TGTGGCTGTA TCAAAATATG AAAGCAGTCT TCCATCAGAA 
ACAGAGGGAG CCCCAGAGAT TGAGCCTGAT ATTCTTCCCA CAAGTGCTTG GATCACCATG 
GTTCTTGACA CCCTTTTGAG TACTGTGGAC AATCATTATC AACCGAAAGG AAGAATCTTC 
CCTTGGTTCC AACATAAATC TTACTCTGGA GAAAACACAG CGAGTGTTAT TGCTAGGTCC 
TGTGCTGCCA CAGCTCTTTC CCTGCTTGTA CCCGTACTTG TTGTTTCTAA TAAAGAGAAG 
GTGGAAGATA TTCTTAACAT CTTGACAGCC AGGCTACCTG GAAAGCCATT AGCAGATGAC 
TCTCAGTCGC TTCAGATACA CATGGGACTT GCGGTTGGCA TGTTCATTTC CAGATTGTTC 
GAGGAGAAAG TCAGTGATGT ATCTGGTCGG CAAATGAATC TACTAATGAT GAGGTCTCTG 
GATGCACTGG AAAGTTGCTG CTTTGACCAA AGCCTGGAAT ACAACATGGG CTGCATTCTG 
GGAGTGGGCC TTGTTTTGTC TTTTATGTGT CAGAGCAGCC AAACTGATAG CCAGGTTCAT 
GTTTCTACTA CTCTGCGCAA ATTTTATATG GCCTTGAGTG AAGACAAGAG CAGAACAGAG 
CAAGAAGGTT TGTCGTACTC TTTGGCCTGT GGGACAGTGT CGGCGTTCAG TGCTGGAATC 
CTTAGTGCTG AAGAAGCTGA AGAGCATATG AACAAGCTGC GTTTGATGAC AGAGCAAAAT 
CAACAGACTC CTGGCTTAGC TCTGGCACTT GGAAGTATTG TCCATGGTTT GTCCATTTGT 
GGACATGGGA AGGCAGAAGA TCTAAATAAC AGATTGCTGC CTGCCTGGAT GAAGATTCTT 
CTTGCAGAGG GTTGCCCCAC AATGCAACGG CTGGCGGCTA TTAATGGCCT TGTGGCACTG 
GTAGGATCAG AATCTGCATT GATACAACTT AAATCTGAAG TAATCCATTC CTCACAGTTT 
CAAAGCAAGC TTAACGAAGT AATTAGAGCC ATAACTCAGA TTGTTAGTTT TTCTGGAGCA 
ATCGGACTGC AGACAAATGC CGCATGGATT CTTGGTCATC TACACTTATC CAGCATTTCA 
TCTTCTCAAA GCAGAACGTC TATTCCTCCA GATTTCAGCT ATCTCCCTGA AAGAAGTCTA 
ATTCGGGCTG TAGTGGACTT TCTTATTTCC GCTGGCAAGA AAGGCCCTGA ATCTCTTCCA 
CCTTGCTTAG TGAAGGTCTG TATATCTTCC ATTGCTGCTG CTGCAGAGGG ACATCAATAT 
CCACCCGTTA ACTGGGCATC CATTCTCGCC CCTCTAATGA GACTCAACTT TGGGGAAGAT 
ACCCAGCAAC TTTGTATTCA AATAGCGGTG ACACAGGCCC AAAGCTCACA GAATGCTGCA 
CTGCTTCTTG GGATGTGGCT GGTGCCCCCA CTGGTTTATA GTCTAACAGT ACAGTGTCGT 
TCCTATCTTT TAAACTCCCT ATCTCTGTGG ATGAAGCATG TATCTGAAGA TAAGCTACAG 
ACCTTCTCTG ACTTATTTAT AGTGACTCAG TTTGAGACCC AAAGCAAGAT GGTGGATTTG 
TGTTGGAGCA TTCTACAGGG ACTTAAGCAA GCTATGAAAC TTCCAAACCC CCCGCAGCAT 
TGCTGGAGCT TTCTGTGCAA AACCACAGAG AAAATTGTTC ACCTTTTACC AAGCAAGATT 
CAGAAGACAG ATGTTGGACT GTATGTTGAA GTTGCACAGT GTGTGTCAGA GATGGCAGAT 
AGTGAGGTTG AGCGGATATC TCGCATTTCA AAGGACAACA TAGAGAAAGC TACCTTTTTG 
CGAGCATATC TGGTCTCACA GGGGCGCTTA CCATTGTCAT GCTTGAATGA TGTTATTGAA 
GTGGCAACCA AGTGCAGGGA GAAACAAACC ATCGTATGGA TGCTGCTGCA GGTGTTCTAC 
CAAGCCCGTC TCACAAGTCA CCCAAACACA GGAGTTCTGA AAAGGATGGA ATGGCTGCTT 
GACCTAACTG TTCATATTAG AAATGCAGTC AATCAGAACA TTTCCGTGAG CAGTGTGGAT 
CACTCAGAGG AAGTAGAGTT CCTGCTGCAG GTATTTGCAG CCTCAGTGGT GTCATGGGCT 
GATCATGCAT CTCCCCTGCT TATAGGAGTC AGTGCCAACT GGATGGCATG GAAGGAGGAA 
GAACCATGTA GTACATATTT GGGCACGGGT TCAGTGGATG TGGTAACTGT ACAGCAGTGT 
TTAACCATCC TTCCCCACAG TATGCAACTC TTGGTGGCCA AAGAGCCTTG GAAAGAACAT 
GCCCAAAAGA TCATCGATTG GCTAACAAAC CTTCTTGAGT GTCCCAAGGA AGCCATATTG 
GAAACATCAA GATCCATCTT AAAAGCCACG CTGCTAAGCC TAAGGGGCCT TCCAGAGTTT 
AAGAGGAAAG TTGTGTGGAC AAGGGCCTAC GGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361602.1
CDS	117..5555
Translation	MATPFTETSVQHACFPKMSEDLKKRLEFPNSLIQTQAVTQLIASVLKENVSLGKIKQSSNQTPALNILWEKCCSNNVVVRTACCEALVLLVEQDHADFDYVLNGILNLIPSAGTVQGLLKCVWRLLYTQARNAEKDGEHKDLGIYKIWNPPHPFISILENRPDCWPALLQQISTLPQLCHESCPSSNVYFMASFLRYLYCEPSQLQEYSALRIGLRKALVMVSGKSGSNNPSKPEEHILRIFIDMMGYIQFLDSTQVTEATLYIKDLCRSLHLHPDFWRNELSAVCLQALCACEASLKIAGDCSTLIEILEQNSDLLGESFPTEQVLIGLSLLLLQSPASQQKLILKLVMKILSFRGENNFPSIVQVLIMPLLQILSTSALGYCFSDDGGSSREQLATSLLEMVQNADCSGERRLNDVSLPLPVTTWHCSMAATLNILNKFQDDHSAARDWLSSISSMLAPNTQVPVNVSLLLSFLLIRESDYGLRNVLKVITDVSKADSSQVPYLVPVLMLRLGHSLAPESSRDILYTLPTLGTHKVCVAQILRVFQMLGSTSKLQPIALRLMTSLWEKQDRIYPELQKMMALCDTQSLATGKDTLWEKMLAKAATVRDICRQRPYQHGADMLAAITQVLHECTKADQATPAALALQGLHALCQAEVVDIRSAWNALSSKLSCETRPLVLKVLHELFALVPSLTVDTEEYQKFKTEIIGILWRHTQSKDLNVMVSAYKSLCEFEADEHSILHIPEQARPEVAPKDESDLASEEEEKETDISIPGSSYIKLMALTPVSVLPALEAFLSSLVKQEMSRMPRGIYHTALRGGNIRSDQGKTIAGVPNFMLKMYEKNKQPGLKPGLAAGMLLCYDLPIQTDKDGRPINRFLASRGRSYQQMLTALINEVNIQSSEWHRSILLPQSWLGFMTRTYHAVLQGRQAELEMQLKHGKDDPQELQYKTFTAWLWVRDALTDVIRCAVKDSPVVKGNSLLALTGLAVAVSKYESSLPSETEGAPEIEPDILPTSAWITMVLDTLLSTVDNHYQPKGRIFPWFQHKSYSGENTASVIARSCAATALSLLVPVLVVSNKEKVEDILNILTARLPGKPLADDSQSLQIHMGLAVGMFISRLFEEKVSDVSGRQMNLLMMRSLDALESCCFDQSLEYNMGCILGVGLVLSFMCQSSQTDSQVHVSTTLRKFYMALSEDKSRTEQEGLSYSLACGTVSAFSAGILSAEEAEEHMNKLRLMTEQNQQTPGLALALGSIVHGLSICGHGKAEDLNNRLLPAWMKILLAEGCPTMQRLAAINGLVALVGSESALIQLKSEVIHSSQFQSKLNEVIRAITQIVSFSGAIGLQTNAAWILGHLHLSSISSSQSRTSIPPDFSYLPERSLIRAVVDFLISAGKKGPESLPPCLVKVCISSIAAAAEGHQYPPVNWASILAPLMRLNFGEDTQQLCIQIAVTQAQSSQNAALLLGMWLVPPLVYSLTVQCRSYLLNSLSLWMKHVSEDKLQTFSDLFIVTQFETQSKMVDLCWSILQGLKQAMKLPNPPQHCWSFLCKTTEKIVHLLPSKIQKTDVGLYVEVAQCVSEMADSEVERISRISKDNIEKATFLRAYLVSQGRLPLSCLNDVIEVATKCREKQTIVWMLLQVFYQARLTSHPNTGVLKRMEWLLDLTVHIRNAVNQNISVSSVDHSEEVEFLLQVFAASVVSWADHASPLLIGVSANWMAWKEEEPCSTYLGTGSVDVVTVQQCLTILPHSMQLLVAKEPWKEHAQKIIDWLTNLLECPKEAILETSRSILKATLLSLRGLPEFKRKVVWTRAYGW

Target ORF information:

RefSeq Version	NM_001374673.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis focadhesin (focad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374673.1

ORF Insert Sequence:

ATGGCCACGC CATTCACTGA AACTAGTGTG CAGCATGCAT GTTTCCCCAA GATGTCAGAA 
GATCTCAAGA AGAGATTAGA ATTTCCAAAC TCATTAATTC AGACACAGGC GGTGACTCAG 
CTCATTGCAT CTGTGTTAAA AGAAAATGTC TCATTGGGGA AGATAAAACA GTCTTCAAAT 
CAGACCCCAG CTCTGAATAT TCTTTGGGAG AAGTGCTGCA GTAATAATGT GGTTGTAAGG 
ACAGCATGTT GTGAAGCATT GGTGCTACTG GTTGAACAGG ATCATGCTGA TTTCGATTAT 
GTTCTTAATG GAATTTTGAA CCTTATACCT TCTGCTGGGA CTGTACAAGG ACTGCTGAAA 
TGTGTATGGC GCCTCCTATA TACTCAAGCA AGGAATGCAG AAAAGGATGG GGAACACAAA 
GATTTGGGCA TTTATAAAAT TTGGAACCCC CCTCATCCAT TCATCAGCAT ACTGGAAAAC 
AGACCAGACT GTTGGCCTGC ACTTCTGCAG CAGATTTCTA CTTTGCCTCA GCTGTGTCAT 
GAAAGCTGTC CTTCAAGCAA TGTTTATTTT ATGGCGTCGT TTTTGAGGTA CTTGTATTGT 
GAACCCTCCC AGCTACAAGA GTACTCTGCC CTTCGAATAG GTCTCAGAAA AGCTTTAGTT 
ATGGTATCGG GGAAGAGTGG GTCCAACAAT CCGTCAAAAC CAGAAGAGCA CATTCTTCGG 
ATTTTCATTG ACATGATGGG CTACATTCAG TTTTTGGATT CAACTCAAGT TACTGAAGCA 
ACTTTGTACA TAAAAGATTT ATGCAGAAGC CTCCACCTGC ATCCAGATTT CTGGAGAAAT 
GAGCTCTCTG CAGTTTGCTT GCAGGCCTTA TGTGCATGTG AGGCAAGCTT AAAAATTGCA 
GGAGACTGCT CTACACTAAT AGAAATATTG GAACAAAATT CAGATCTGCT TGGTGAGAGC 
TTTCCCACAG AACAGGTATT AATTGGACTC AGTTTACTAT TGCTACAATC ACCAGCTAGC 
CAGCAAAAAC TAATCTTAAA GTTGGTAATG AAGATTCTGT CATTCAGAGG GGAAAACAAT 
TTTCCATCAA TCGTCCAAGT GTTAATTATG CCACTCCTCC AAATTTTATC TACCTCAGCG 
CTGGGATATT GTTTCTCTGA TGATGGTGGA AGCTCGAGAG AGCAGCTGGC TACTAGTCTT 
CTGGAAATGG TTCAGAATGC TGACTGTAGT GGGGAAAGGA GACTGAATGA TGTATCACTT 
CCTCTTCCTG TAACAACATG GCATTGTTCA ATGGCTGCTA CATTGAACAT ACTTAACAAG 
TTTCAAGATG ACCATTCTGC AGCACGTGAC TGGCTGTCTT CCATATCTTC GATGCTGGCC 
CCCAATACTC AAGTTCCTGT CAATGTTTCA CTTTTGCTAA GTTTCCTTCT GATTAGAGAA 
TCTGATTATG GCTTGCGTAA TGTTTTGAAA GTCATCACTG ATGTCTCCAA AGCTGATTCA 
TCTCAGGTTC CATATTTAGT TCCAGTTCTA ATGTTGAGAC TTGGACATTC CTTGGCACCA 
GAATCCTCCA GAGACATTCT GTACACTTTG CCTACCCTGG GCACCCACAA GGTATGTGTG 
GCTCAGATCT TGCGAGTCTT CCAAATGTTA GGAAGTACTT CAAAGCTTCA ACCCATTGCG 
CTTCGTCTTA TGACATCTTT GTGGGAGAAA CAGGACCGAA TCTATCCAGA ATTACAGAAG 
ATGATGGCCT TATGTGACAC TCAGTCATTA GCCACTGGGA AAGACACATT GTGGGAAAAG 
ATGTTGGCAA AAGCAGCAAC TGTCAGAGAT ATCTGCAGAC AGAGGCCATA TCAGCATGGG 
GCAGATATGC TGGCTGCTAT AACGCAAGTT TTACATGAAT GCACAAAAGC CGACCAAGCT 
ACTCCGGCTG CTTTAGCATT GCAGGGTCTT CATGCTCTCT GTCAAGCAGA GGTTGTAGAC 
ATTCGCTCTG CCTGGAATGC TCTTTCCTCC AAACTTAGTT GTGAAACACG TCCCCTTGTC 
CTCAAAGTGT TACATGAACT TTTTGCATTG GTCCCTTCTT TGACTGTTGA TACAGAAGAA 
TATCAGAAAT TTAAAACTGA GATTATCGGT ATCCTTTGGA GGCACACTCA AAGTAAGGAT 
CTCAATGTGA TGGTTTCTGC ATACAAATCT TTATGTGAGT TTGAGGCTGA TGAGCACTCC 
ATTCTTCATA TTCCTGAGCA GGCAAGGCCT GAGGTGGCAC CTAAAGATGA ATCTGACCTG 
GCTAGTGAAG AAGAGGAGAA AGAAACTGAC ATTTCTATCC CTGGCTCATC ATATATAAAA 
TTAATGGCTT TGACACCGGT ATCTGTTTTA CCAGCTCTTG AAGCTTTCCT TTCATCTCTT 
GTAAAGCAAG AAATGAGCCG CATGCCACGT GGTATTTATC ATACAGCTCT GCGTGGTGGT 
AATATTAGAT CTGACCAAGG CAAAACTATT GCAGGAGTCC CAAACTTCAT GCTAAAAATG 
TACGAGAAGA ATAAACAGCC TGGCCTGAAA CCTGGCCTTG CAGCTGGCAT GCTGCTGTGT 
TACGATCTCC CAATCCAGAC AGACAAGGAT GGCAGGCCCA TTAATCGTTT CCTTGCCAGT 
AGAGGGCGCA GCTACCAGCA GATGTTAACC GCACTTATCA ATGAGGTGAA TATTCAGTCT 
TCAGAATGGC ATCGCTCTAT ATTGCTGCCC CAGTCATGGC TTGGCTTCAT GACAAGGACA 
TACCATGCTG TTCTGCAAGG AAGGCAAGCT GAACTGGAAA TGCAGCTGAA ACATGGAAAG 
GATGACCCAC AAGAGCTTCA GTACAAAACA TTTACAGCTT GGTTGTGGGT AAGGGATGCA 
TTGACCGATG TCATTAGGTG TGCTGTGAAA GACAGTCCTG TGGTGAAGGG AAATTCTCTG 
TTGGCATTGA CTGGACTTGC TGTGGCTGTA TCAAAATATG AAAGCAGTCT TCCATCAGAA 
ACAGAGGGAG CCCCAGAGAT TGAGCCTGAT ATTCTTCCCA CAAGTGCTTG GATCACCATG 
GTTCTTGACA CCCTTTTGAG TACTGTGGAC AATCATTATC AACCGAAAGG AAGAATCTTC 
CCTTGGTTCC AACATAAATC TTACTCTGGA GAAAACACAG CGAGTGTTAT TGCTAGGTCC 
TGTGCTGCCA CAGCTCTTTC CCTGCTTGTA CCCGTACTTG TTGTTTCTAA TAAAGAGAAG 
GTGGAAGATA TTCTTAACAT CTTGACAGCC AGGCTACCTG GAAAGCCATT AGCAGATGAC 
TCTCAGTCGC TTCAGATACA CATGGGACTT GCGGTTGGCA TGTTCATTTC CAGATTGTTC 
GAGGAGAAAG TCAGTGATGT ATCTGGTCGG CAAATGAATC TACTAATGAT GAGGTCTCTG 
GATGCACTGG AAAGTTGCTG CTTTGACCAA AGCCTGGAAT ACAACATGGG CTGCATTCTG 
GGAGTGGGCC TTGTTTTGTC TTTTATGTGT CAGAGCAGCC AAACTGATAG CCAGGTTCAT 
GTTTCTACTA CTCTGCGCAA ATTTTATATG GCCTTGAGTG AAGACAAGAG CAGAACAGAG 
CAAGAAGGTT TGTCGTACTC TTTGGCCTGT GGGACAGTGT CGGCGTTCAG TGCTGGAATC 
CTTAGTGCTG AAGAAGCTGA AGAGCATATG AACAAGCTGC GTTTGATGAC AGAGCAAAAT 
CAACAGACTC CTGGCTTAGC TCTGGCACTT GGAAGTATTG TCCATGGTTT GTCCATTTGT 
GGACATGGGA AGGCAGAAGA TCTAAATAAC AGATTGCTGC CTGCCTGGAT GAAGATTCTT 
CTTGCAGAGG GTTGCCCCAC AATGCAACGG CTGGCGGCTA TTAATGGCCT TGTGGCACTG 
GTAGGATCAG AATCTGCATT GATACAACTT AAATCTGAAG TAATCCATTC CTCACAGTTT 
CAAAGCAAGC TTAACGAAGT AATTAGAGCC ATAACTCAGA TTGTTAGTTT TTCTGGAGCA 
ATCGGACTGC AGACAAATGC CGCATGGATT CTTGGTCATC TACACTTATC CAGCATTTCA 
TCTTCTCAAA GCAGAACGTC TATTCCTCCA GATTTCAGCT ATCTCCCTGA AAGAAGTCTA 
ATTCGGGCTG TAGTGGACTT TCTTATTTCC GCTGGCAAGA AAGGCCCTGA ATCTCTTCCA 
CCTTGCTTAG TGAAGGTCTG TATATCTTCC ATTGCTGCTG CTGCAGAGGG ACATCAATAT 
CCACCCGTTA ACTGGGCATC CATTCTCGCC CCTCTAATGA GACTCAACTT TGGGGAAGAT 
ACCCAGCAAC TTTGTATTCA AATAGCGGTG ACACAGGCCC AAAGCTCACA GAATGCTGCA 
CTGCTTCTTG GGATGTGGCT GGTGCCCCCA CTGGTTTATA GTCTAACAGT ACAGTGTCGT 
TCCTATCTTT TAAACTCCCT ATCTCTGTGG ATGAAGCATG TATCTGAAGA TAAGCTACAG 
ACCTTCTCTG ACTTATTTAT AGTGACTCAG TTTGAGACCC AAAGCAAGAT GGTGGATTTG 
TGTTGGAGCA TTCTACAGGG ACTTAAGCAA GCTATGAAAC TTCCAAACCC CCCGCAGCAT 
TGCTGGAGCT TTCTGTGCAA AACCACAGAG AAAATTGTTC ACCTTTTACC AAGCAAGATT 
CAGAAGACAG ATGTTGGACT GTATGTTGAA GTTGCACAGT GTGTGTCAGA GATGGCAGAT 
AGTGAGGTTG AGCGGATATC TCGCATTTCA AAGGACAACA TAGAGAAAGC TACCTTTTTG 
CGAGCATATC TGGTCTCACA GGGGCGCTTA CCATTGTCAT GCTTGAATGA TGTTATTGAA 
GTGGCAACCA AGTGCAGGGA GAAACAAACC ATCGTATGGA TGCTGCTGCA GGTGTTCTAC 
CAAGCCCGTC TCACAAGTCA CCCAAACACA GGAGTTCTGA AAAGGATGGA ATGGCTGCTT 
GACCTAACTG TTCATATTAG AAATGCAGTC AATCAGAACA TTTCCGTGAG CAGTGTGGAT 
CACTCAGAGG AAGTAGAGTT CCTGCTGCAG GTATTTGCAG CCTCAGTGGT GTCATGGGCT 
GATCATGCAT CTCCCCTGCT TATAGGAGTC AGTGCCAACT GGATGGCATG GAAGGAGGAA 
GAACCATGTA GTACATATTT GGGCACGGGT TCAGTGGATG TGGTAACTGT ACAGCAGTGT 
TTAACCATCC TTCCCCACAG TATGCAACTC TTGGTGGCCA AAGAGCCTTG GAAAGAACAT 
GCCCAAAAGA TCATCGATTG GCTAACAAAC CTTCTTGAGT GTCCCAAGGA AGCCATATTG 
GAAACATCAA GATCCATCTT AAAAGCCACG CTGCTAAGCC TAAGGGGCCT TCCAGAGTTT 
AAGAGGAAAG TTGTGTGGAC AAGGGCCTAC GGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

focad ( XM_012962109.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817563.1 Xenopus tropicalis focadhesin (focad), transcript variant X1, mRNA.

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CloneID	OXa27622
Clone ID Related Accession (Same CDS sequence)	XM_012962109.2 , XM_012962109.3
Accession Version	XM_012962109.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	focadhesin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962109.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAG ATCTCAAGAA GAGATTAGAA TTTCCAAACT CATTAATTCA GACACAGGCG 
GTGACTCAGC TCATTGCATC TGTGTTAAAA GAAAATGTCT CATTGGGGAA GATAAAACAG 
TCTTCAAATC AGACCCCAGC TCTGAATATT CTTTGGGAGA AGTGCTGCAG TAATAATGTG 
GTTGTAAGGA CAGCATGTTG TGAAGCATTG GTGCTACTGG TTGAACAGGA TCATGCTGAT 
TTCGATTATG TTCTTAATGG AATTTTGAAC CTTATACCTT CTGCTGGGAC TGTACAAGGA 
CTGCTGAAAT GTGTATGGCG CCTCCTATAT ACTCAAGCAA GGAATGCAGA AAAGGATGGG 
GAACACAAAG ATTTGGGCAT TTATAAAATT TGGAACCCCC CTCATCCATT CATCAGCATA 
CTGGAAAACA GACCAGACTG TTGGCCTGCA CTTCTGCAGC AGATTTCTAC TTTGCCTCAG 
CTGTGTCATG AAAGCTGTCC TTCAAGCAAT GTTTATTTTA TGGCGTCGTT TTTGAGGTAC 
TTGTATTGTG AACCCTCCCA GCTACAAGAG TACTCTGCCC TTCGAATAGG TCTCAGAAAA 
GCTTTAGTTA TGGTATCGGG GAAGAGTGGG TCCAACAATC CGTCAAAACC AGAAGAGCAC 
ATTCTTCGGA TTTTCATTGA CATGATGGGC TACATTCAGT TTTTGGATTC AACTCAAGTT 
ACTGAAGCAA CTTTGTACAT AAAAGATTTA TGCAGAAGCC TCCACCTGCA TCCAGATTTC 
TGGAGAAATG AGCTCTCTGC AGTTTGCTTG CAGGCCTTAT GTGCATGTGA GGCAAGCTTA 
AAAATTGCAG GAGACTGCTC TACACTAATA GAAATATTGG AACAAAATTC AGATCTGCTT 
GGTGAGAGCT TTCCCACAGA ACAGGTATTA ATTGGACTCA GTTTACTATT GCTACAATCA 
CCAGCTAGCC AGCAAAAACT AATCTTAAAG TTGGTAATGA AGATTCTGTC ATTCAGAGGG 
GAAAACAATT TTCCATCAAT CGTCCAAGTG TTAATTATGC CACTCCTCCA AATTTTATCT 
ACCTCAGCGC TGGGATATTG TTTCTCTGAT GATGGTGGAA GCTCGAGAGA GCAGCTGGCT 
ACTAGTCTTC TGGAAATGGT TCAGAATGCT GACTGTAGTG GGGAAAGGAG ACTGAATGAT 
GTATCACTTC CTCTTCCTGT AACAACATGG CATTGTTCAA TGGCTGCTAC ATTGAACATA 
CTTAACAAGT TTCAAGATGA CCATTCTGCA GCACGTGACT GGCTGTCTTC CATATCTTCG 
ATGCTGGCCC CCAATACTCA AGTTCCTGTC AATGTTTCAC TTTTGCTAAG TTTCCTTCTG 
ATTAGAGAAT CTGATTATGG CTTGCGTAAT GTTTTGAAAG TCATCACTGA TGTCTCCAAA 
GCTGATTCAT CTCAGGTTCC ATATTTAGTT CCAGTTCTAA TGTTGAGACT TGGACATTCC 
TTGGCACCAG AATCCTCCAG AGACATTCTG TACACTTTGC CTACCCTGGG CACCCACAAG 
GTATGTGTGG CTCAGATCTT GCGAGTCTTC CAAATGTTAG GAAGTACTTC AAAGCTTCAA 
CCCATTGCGC TTCGTCTTAT GACATCTTTG TGGGAGAAAC AGGACCGAAT CTATCCAGAA 
TTACAGAAGA TGATGGCCTT ATGTGACACT CAGTCATTAG CCACTGGGAA AGACACATTG 
TGGGAAAAGA TGTTGGCAAA AGCAGCAACT GTCAGAGATA TCTGCAGACA GAGGCCATAT 
CAGCATGGGG CAGATATGCT GGCTGCTATA ACGCAAGTTT TACATGAATG CACAAAAGCC 
GACCAAGCTA CTCCGGCTGC TTTAGCATTG CAGGGTCTTC ATGCTCTCTG TCAAGCAGAG 
GTTGTAGACA TTCGCTCTGC CTGGAATGCT CTTTCCTCCA AACTTAGTTG TGAAACACGT 
CCCCTTGTCC TCAAAGTGTT ACATGAACTT TTTGCATTGG TCCCTTCTTT GACTGTTGAT 
ACAGAAGAAT ATCAGAAATT TAAAACTGAG ATTATCGGTA TCCTTTGGAG GCACACTCAA 
AGTAAGGATC TCAATGTGAT GGTTTCTGCA TACAAATCTT TATGTGAGTT TGAGGCTGAT 
GAGCACTCCA TTCTTCATAT TCCTGAGCAG GCAAGGCCTG AGGTGGCACC TAAAGATGAA 
TCTGACCTGG CTAGTGAAGA AGAGGAGAAA GAAACTGACA TTTCTATCCC TGGCTCATCA 
TATATAAAAT TAATGGCTTT GACACCGGTA TCTGTTTTAC CAGCTCTTGA AGCTTTCCTT 
TCATCTCTTG TAAAGCAAGA AATGAGCCGC ATGCCACGTG GTATTTATCA TACAGCTCTG 
CGTGGTGGTA ATATTAGATC TGACCAAGGC AAAACTATTG CAGGAGTCCC AAACTTCATG 
CTAAAAATGT ACGAGAAGAA TAAACAGCCT GGCCTGAAAC CTGGCCTTGC AGCTGGCATG 
CTGCTGTGTT ACGATCTCCC AATCCAGACA GACAAGGATG GCAGGCCCAT TAATCGTTTC 
CTTGCCAGTA GAGGGCGCAG CTACCAGCAG ATGTTAACCG CACTTATCAA TGAGGTGAAT 
ATTCAGTCTT CAGAATGGCA TCGCTCTATA TTGCTGCCCC AGTCATGGCT TGGCTTCATG 
ACAAGGACAT ACCATGCTGT TCTGCAAGGA AGGCAAGCTG AACTGGAAAT GCAGCTGAAA 
CATGGAAAGG ATGACCCACA AGAGCTTCAG TACAAAACAT TTACAGCTTG GTTGTGGGTA 
AGGGATGCAT TGACCGATGT CATTAGGTGT GCTGTGAAAG ACAGTCCTGT GGTGAAGGGA 
AATTCTCTGT TGGCATTGAC TGGACTTGCT GTGGCTGTAT CAAAATATGA AAGCAGTCTT 
CCATCAGAAA CAGAGGGAGC CCCAGAGATT GAGCCTGATA TTCTTCCCAC AAGTGCTTGG 
ATCACCATGG TTCTTGACAC CCTTTTGAGT ACTGTGGACA ATCATTATCA ACCGAAAGGA 
AGAATCTTCC CTTGGTTCCA ACATAAATCT TACTCTGGAG AAAACACAGC GAGTGTTATT 
GCTAGGTCCT GTGCTGCCAC AGCTCTTTCC CTGCTTGTAC CCGTACTTGT TGTTTCTAAT 
AAAGAGAAGG TGGAAGATAT TCTTAACATC TTGACAGCCA GGCTACCTGG AAAGCCATTA 
GCAGATGACT CTCAGTCGCT TCAGATACAC ATGGGACTTG CGGTTGGCAT GTTCATTTCC 
AGATTGTTCG AGGAGAAAGT CAGTGATGTA TCTGGTCGGC AAATGAATCT ACTAATGATG 
AGGTCTCTGG ATGCACTGGA AAGTTGCTGC TTTGACCAAA GCCTGGAATA CAACATGGGC 
TGCATTCTGG GAGTGGGCCT TGTTTTGTCT TTTATGTGTC AGAGCAGCCA AACTGATAGC 
CAGGTTCATG TTTCTACTAC TCTGCGCAAA TTTTATATGG CCTTGAGTGA AGACAAGAGC 
AGAACAGAGC AAGAAGGTTT GTCGTACTCT TTGGCCTGTG GGACAGTGTC GGCGTTCAGT 
GCTGGAATCC TTAGTGCTGA AGAAGCTGAA GAGCATATGA ACAAGCTGCG TTTGATGACA 
GAGCAAAATC AACAGACTCC TGGCTTAGCT CTGGCACTTG GAAGTATTGT CCATGGTTTG 
TCCATTTGTG GACATGGGAA GGCAGAAGAT CTAAATAACA GATTGCTGCC TGCCTGGATG 
AAGATTCTTC TTGCAGAGGG TTGCCCCACA ATGCAACGGC TGGCGGCTAT TAATGGCCTT 
GTGGCACTGG TAGGATCAGA ATCTGCATTG ATACAACTTA AATCTGAAGT AATCCATTCC 
TCACAGTTTC AAAGCAAGCT TAACGAAGTA ATTAGAGCCA TAACTCAGAT TGTTAGTTTT 
TCTGGAGCAA TCGGACTGCA GACAAATGCC GCATGGATTC TTGGTCATCT ACACTTATCC 
AGCATTTCAT CTTCTCAAAG CAGAACGTCT ATTCCTCCAG ATTTCAGCTA TCTCCCTGAA 
AGAAGTCTAA TTCGGGCTGT AGTGGACTTT CTTATTTCCG CTGGCAAGAA AGGCCCTGAA 
TCTCTTCCAC CTTGCTTAGT GAAGGTCTGT ATATCTTCCA TTGCTGCTGC TGCAGAGGGA 
CATCAATATC CACCCGTTAA CTGGGCATCC ATTCTCGCCC CTCTAATGAG ACTCAACTTT 
GGGGAAGATA CCCAGCAACT TTGTATTCAA ATAGCGGTGA CACAGGCCCA AAGCTCACAG 
AATGCTGCAC TGCTTCTTGG GATGTGGCTG GTGCCCCCAC TGGTTTATAG TCTAACAGTA 
CAGTGTCGTT CCTATCTTTT AAACTCCCTA TCTCTGTGGA TGAAGCATGT ATCTGAAGAT 
AAGCTACAGA CCTTCTCTGA CTTATTTATA GTGACTCAGT TTGAGACCCA AAGCAAGATG 
GTGGATTTGT GTTGGAGCAT TCTACAGGGA CTTAAGCAAG CTATGAAACT TCCAAACCCC 
CCGCAGCATT GCTGGAGCTT TCTGTGCAAA ACCACAGAGA AAATTGTTCA CCTTTTACCA 
AGCAAGATTC AGAAGACAGA TGTTGGACTG TATGTTGAAG TTGCACAGTG TGTGTCAGAG 
ATGGCAGATA GTGAGGTTGA GCGGATATCT CGCATTTCAA AGGACAACAT AGAGAAAGCT 
ACCTTTTTGC GAGCATATCT GGTCTCACAG GGGCGCTTAC CATTGTCATG CTTGAATGAT 
GTTATTGAAG TGGCAACCAA GTGCAGGGAG AAACAAACCA TCGTATGGAT GCTGCTGCAG 
GTGTTCTACC AAGCCCGTCT CACAAGTCAC CCAAACACAG GAGTTCTGAA AAGGATGGAA 
TGGCTGCTTG ACCTAACTGT TCATATTAGA AATGCAGTCA ATCAGAACAT TTCCGTGAGC 
AGTGTGGATC ACTCAGAGGA AGTAGAGTTC CTGCTGCAGG TATTTGCAGC CTCAGTGGTG 
TCATGGGCTG ATCATGCATC TCCCCTGCTT ATAGGAGTCA GTGCCAACTG GATGGCATGG 
AAGGAGGAAG AACCATGTAG TACATATTTG GGCACGGGTT CAGTGGATGT GGTAACTGTA 
CAGCAGTGTT TAACCATCCT TCCCCACAGT ATGCAACTCT TGGTGGCCAA AGAGCCTTGG 
AAAGAACATG CCCAAAAGAT CATCGATTGG CTAACAAACC TTCTTGAGTG TCCCAAGGAA 
GCCATATTGG AAACATCAAG ATCCATCTTA AAAGCCACGC TGCTAAGCCT AAGGGGCCTT 
CCAGAGTTTA AGAGGAAAGT TGTGTGGACA AGGGCCTACG GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817563.1
CDS	279..5666
Translation	MSEDLKKRLEFPNSLIQTQAVTQLIASVLKENVSLGKIKQSSNQTPALNILWEKCCSNNVVVRTACCEALVLLVEQDHADFDYVLNGILNLIPSAGTVQGLLKCVWRLLYTQARNAEKDGEHKDLGIYKIWNPPHPFISILENRPDCWPALLQQISTLPQLCHESCPSSNVYFMASFLRYLYCEPSQLQEYSALRIGLRKALVMVSGKSGSNNPSKPEEHILRIFIDMMGYIQFLDSTQVTEATLYIKDLCRSLHLHPDFWRNELSAVCLQALCACEASLKIAGDCSTLIEILEQNSDLLGESFPTEQVLIGLSLLLLQSPASQQKLILKLVMKILSFRGENNFPSIVQVLIMPLLQILSTSALGYCFSDDGGSSREQLATSLLEMVQNADCSGERRLNDVSLPLPVTTWHCSMAATLNILNKFQDDHSAARDWLSSISSMLAPNTQVPVNVSLLLSFLLIRESDYGLRNVLKVITDVSKADSSQVPYLVPVLMLRLGHSLAPESSRDILYTLPTLGTHKVCVAQILRVFQMLGSTSKLQPIALRLMTSLWEKQDRIYPELQKMMALCDTQSLATGKDTLWEKMLAKAATVRDICRQRPYQHGADMLAAITQVLHECTKADQATPAALALQGLHALCQAEVVDIRSAWNALSSKLSCETRPLVLKVLHELFALVPSLTVDTEEYQKFKTEIIGILWRHTQSKDLNVMVSAYKSLCEFEADEHSILHIPEQARPEVAPKDESDLASEEEEKETDISIPGSSYIKLMALTPVSVLPALEAFLSSLVKQEMSRMPRGIYHTALRGGNIRSDQGKTIAGVPNFMLKMYEKNKQPGLKPGLAAGMLLCYDLPIQTDKDGRPINRFLASRGRSYQQMLTALINEVNIQSSEWHRSILLPQSWLGFMTRTYHAVLQGRQAELEMQLKHGKDDPQELQYKTFTAWLWVRDALTDVIRCAVKDSPVVKGNSLLALTGLAVAVSKYESSLPSETEGAPEIEPDILPTSAWITMVLDTLLSTVDNHYQPKGRIFPWFQHKSYSGENTASVIARSCAATALSLLVPVLVVSNKEKVEDILNILTARLPGKPLADDSQSLQIHMGLAVGMFISRLFEEKVSDVSGRQMNLLMMRSLDALESCCFDQSLEYNMGCILGVGLVLSFMCQSSQTDSQVHVSTTLRKFYMALSEDKSRTEQEGLSYSLACGTVSAFSAGILSAEEAEEHMNKLRLMTEQNQQTPGLALALGSIVHGLSICGHGKAEDLNNRLLPAWMKILLAEGCPTMQRLAAINGLVALVGSESALIQLKSEVIHSSQFQSKLNEVIRAITQIVSFSGAIGLQTNAAWILGHLHLSSISSSQSRTSIPPDFSYLPERSLIRAVVDFLISAGKKGPESLPPCLVKVCISSIAAAAEGHQYPPVNWASILAPLMRLNFGEDTQQLCIQIAVTQAQSSQNAALLLGMWLVPPLVYSLTVQCRSYLLNSLSLWMKHVSEDKLQTFSDLFIVTQFETQSKMVDLCWSILQGLKQAMKLPNPPQHCWSFLCKTTEKIVHLLPSKIQKTDVGLYVEVAQCVSEMADSEVERISRISKDNIEKATFLRAYLVSQGRLPLSCLNDVIEVATKCREKQTIVWMLLQVFYQARLTSHPNTGVLKRMEWLLDLTVHIRNAVNQNISVSSVDHSEEVEFLLQVFAASVVSWADHASPLLIGVSANWMAWKEEEPCSTYLGTGSVDVVTVQQCLTILPHSMQLLVAKEPWKEHAQKIIDWLTNLLECPKEAILETSRSILKATLLSLRGLPEFKRKVVWTRAYGW

Target ORF information:

RefSeq Version	XM_012962109.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis focadhesin (focad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962109.3

ORF Insert Sequence:

ATGTCAGAAG ATCTCAAGAA GAGATTAGAA TTTCCAAACT CATTAATTCA GACACAGGCG 
GTGACTCAGC TCATTGCATC TGTGTTAAAA GAAAATGTCT CATTGGGGAA GATAAAACAG 
TCTTCAAATC AGACCCCAGC TCTGAATATT CTTTGGGAGA AGTGCTGCAG TAATAATGTG 
GTTGTAAGGA CAGCATGTTG TGAAGCATTG GTGCTACTGG TTGAACAGGA TCATGCTGAT 
TTCGATTATG TTCTTAATGG AATTTTGAAC CTTATACCTT CTGCTGGGAC TGTACAAGGA 
CTGCTGAAAT GTGTATGGCG CCTCCTATAT ACTCAAGCAA GGAATGCAGA AAAGGATGGG 
GAACACAAAG ATTTGGGCAT TTATAAAATT TGGAACCCCC CTCATCCATT CATCAGCATA 
CTGGAAAACA GACCAGACTG TTGGCCTGCA CTTCTGCAGC AGATTTCTAC TTTGCCTCAG 
CTGTGTCATG AAAGCTGTCC TTCAAGCAAT GTTTATTTTA TGGCGTCGTT TTTGAGGTAC 
TTGTATTGTG AACCCTCCCA GCTACAAGAG TACTCTGCCC TTCGAATAGG TCTCAGAAAA 
GCTTTAGTTA TGGTATCGGG GAAGAGTGGG TCCAACAATC CGTCAAAACC AGAAGAGCAC 
ATTCTTCGGA TTTTCATTGA CATGATGGGC TACATTCAGT TTTTGGATTC AACTCAAGTT 
ACTGAAGCAA CTTTGTACAT AAAAGATTTA TGCAGAAGCC TCCACCTGCA TCCAGATTTC 
TGGAGAAATG AGCTCTCTGC AGTTTGCTTG CAGGCCTTAT GTGCATGTGA GGCAAGCTTA 
AAAATTGCAG GAGACTGCTC TACACTAATA GAAATATTGG AACAAAATTC AGATCTGCTT 
GGTGAGAGCT TTCCCACAGA ACAGGTATTA ATTGGACTCA GTTTACTATT GCTACAATCA 
CCAGCTAGCC AGCAAAAACT AATCTTAAAG TTGGTAATGA AGATTCTGTC ATTCAGAGGG 
GAAAACAATT TTCCATCAAT CGTCCAAGTG TTAATTATGC CACTCCTCCA AATTTTATCT 
ACCTCAGCGC TGGGATATTG TTTCTCTGAT GATGGTGGAA GCTCGAGAGA GCAGCTGGCT 
ACTAGTCTTC TGGAAATGGT TCAGAATGCT GACTGTAGTG GGGAAAGGAG ACTGAATGAT 
GTATCACTTC CTCTTCCTGT AACAACATGG CATTGTTCAA TGGCTGCTAC ATTGAACATA 
CTTAACAAGT TTCAAGATGA CCATTCTGCA GCACGTGACT GGCTGTCTTC CATATCTTCG 
ATGCTGGCCC CCAATACTCA AGTTCCTGTC AATGTTTCAC TTTTGCTAAG TTTCCTTCTG 
ATTAGAGAAT CTGATTATGG CTTGCGTAAT GTTTTGAAAG TCATCACTGA TGTCTCCAAA 
GCTGATTCAT CTCAGGTTCC ATATTTAGTT CCAGTTCTAA TGTTGAGACT TGGACATTCC 
TTGGCACCAG AATCCTCCAG AGACATTCTG TACACTTTGC CTACCCTGGG CACCCACAAG 
GTATGTGTGG CTCAGATCTT GCGAGTCTTC CAAATGTTAG GAAGTACTTC AAAGCTTCAA 
CCCATTGCGC TTCGTCTTAT GACATCTTTG TGGGAGAAAC AGGACCGAAT CTATCCAGAA 
TTACAGAAGA TGATGGCCTT ATGTGACACT CAGTCATTAG CCACTGGGAA AGACACATTG 
TGGGAAAAGA TGTTGGCAAA AGCAGCAACT GTCAGAGATA TCTGCAGACA GAGGCCATAT 
CAGCATGGGG CAGATATGCT GGCTGCTATA ACGCAAGTTT TACATGAATG CACAAAAGCC 
GACCAAGCTA CTCCGGCTGC TTTAGCATTG CAGGGTCTTC ATGCTCTCTG TCAAGCAGAG 
GTTGTAGACA TTCGCTCTGC CTGGAATGCT CTTTCCTCCA AACTTAGTTG TGAAACACGT 
CCCCTTGTCC TCAAAGTGTT ACATGAACTT TTTGCATTGG TCCCTTCTTT GACTGTTGAT 
ACAGAAGAAT ATCAGAAATT TAAAACTGAG ATTATCGGTA TCCTTTGGAG GCACACTCAA 
AGTAAGGATC TCAATGTGAT GGTTTCTGCA TACAAATCTT TATGTGAGTT TGAGGCTGAT 
GAGCACTCCA TTCTTCATAT TCCTGAGCAG GCAAGGCCTG AGGTGGCACC TAAAGATGAA 
TCTGACCTGG CTAGTGAAGA AGAGGAGAAA GAAACTGACA TTTCTATCCC TGGCTCATCA 
TATATAAAAT TAATGGCTTT GACACCGGTA TCTGTTTTAC CAGCTCTTGA AGCTTTCCTT 
TCATCTCTTG TAAAGCAAGA AATGAGCCGC ATGCCACGTG GTATTTATCA TACAGCTCTG 
CGTGGTGGTA ATATTAGATC TGACCAAGGC AAAACTATTG CAGGAGTCCC AAACTTCATG 
CTAAAAATGT ACGAGAAGAA TAAACAGCCT GGCCTGAAAC CTGGCCTTGC AGCTGGCATG 
CTGCTGTGTT ACGATCTCCC AATCCAGACA GACAAGGATG GCAGGCCCAT TAATCGTTTC 
CTTGCCAGTA GAGGGCGCAG CTACCAGCAG ATGTTAACCG CACTTATCAA TGAGGTGAAT 
ATTCAGTCTT CAGAATGGCA TCGCTCTATA TTGCTGCCCC AGTCATGGCT TGGCTTCATG 
ACAAGGACAT ACCATGCTGT TCTGCAAGGA AGGCAAGCTG AACTGGAAAT GCAGCTGAAA 
CATGGAAAGG ATGACCCACA AGAGCTTCAG TACAAAACAT TTACAGCTTG GTTGTGGGTA 
AGGGATGCAT TGACCGATGT CATTAGGTGT GCTGTGAAAG ACAGTCCTGT GGTGAAGGGA 
AATTCTCTGT TGGCATTGAC TGGACTTGCT GTGGCTGTAT CAAAATATGA AAGCAGTCTT 
CCATCAGAAA CAGAGGGAGC CCCAGAGATT GAGCCTGATA TTCTTCCCAC AAGTGCTTGG 
ATCACCATGG TTCTTGACAC CCTTTTGAGT ACTGTGGACA ATCATTATCA ACCGAAAGGA 
AGAATCTTCC CTTGGTTCCA ACATAAATCT TACTCTGGAG AAAACACAGC GAGTGTTATT 
GCTAGGTCCT GTGCTGCCAC AGCTCTTTCC CTGCTTGTAC CCGTACTTGT TGTTTCTAAT 
AAAGAGAAGG TGGAAGATAT TCTTAACATC TTGACAGCCA GGCTACCTGG AAAGCCATTA 
GCAGATGACT CTCAGTCGCT TCAGATACAC ATGGGACTTG CGGTTGGCAT GTTCATTTCC 
AGATTGTTCG AGGAGAAAGT CAGTGATGTA TCTGGTCGGC AAATGAATCT ACTAATGATG 
AGGTCTCTGG ATGCACTGGA AAGTTGCTGC TTTGACCAAA GCCTGGAATA CAACATGGGC 
TGCATTCTGG GAGTGGGCCT TGTTTTGTCT TTTATGTGTC AGAGCAGCCA AACTGATAGC 
CAGGTTCATG TTTCTACTAC TCTGCGCAAA TTTTATATGG CCTTGAGTGA AGACAAGAGC 
AGAACAGAGC AAGAAGGTTT GTCGTACTCT TTGGCCTGTG GGACAGTGTC GGCGTTCAGT 
GCTGGAATCC TTAGTGCTGA AGAAGCTGAA GAGCATATGA ACAAGCTGCG TTTGATGACA 
GAGCAAAATC AACAGACTCC TGGCTTAGCT CTGGCACTTG GAAGTATTGT CCATGGTTTG 
TCCATTTGTG GACATGGGAA GGCAGAAGAT CTAAATAACA GATTGCTGCC TGCCTGGATG 
AAGATTCTTC TTGCAGAGGG TTGCCCCACA ATGCAACGGC TGGCGGCTAT TAATGGCCTT 
GTGGCACTGG TAGGATCAGA ATCTGCATTG ATACAACTTA AATCTGAAGT AATCCATTCC 
TCACAGTTTC AAAGCAAGCT TAACGAAGTA ATTAGAGCCA TAACTCAGAT TGTTAGTTTT 
TCTGGAGCAA TCGGACTGCA GACAAATGCC GCATGGATTC TTGGTCATCT ACACTTATCC 
AGCATTTCAT CTTCTCAAAG CAGAACGTCT ATTCCTCCAG ATTTCAGCTA TCTCCCTGAA 
AGAAGTCTAA TTCGGGCTGT AGTGGACTTT CTTATTTCCG CTGGCAAGAA AGGCCCTGAA 
TCTCTTCCAC CTTGCTTAGT GAAGGTCTGT ATATCTTCCA TTGCTGCTGC TGCAGAGGGA 
CATCAATATC CACCCGTTAA CTGGGCATCC ATTCTCGCCC CTCTAATGAG ACTCAACTTT 
GGGGAAGATA CCCAGCAACT TTGTATTCAA ATAGCGGTGA CACAGGCCCA AAGCTCACAG 
AATGCTGCAC TGCTTCTTGG GATGTGGCTG GTGCCCCCAC TGGTTTATAG TCTAACAGTA 
CAGTGTCGTT CCTATCTTTT AAACTCCCTA TCTCTGTGGA TGAAGCATGT ATCTGAAGAT 
AAGCTACAGA CCTTCTCTGA CTTATTTATA GTGACTCAGT TTGAGACCCA AAGCAAGATG 
GTGGATTTGT GTTGGAGCAT TCTACAGGGA CTTAAGCAAG CTATGAAACT TCCAAACCCC 
CCGCAGCATT GCTGGAGCTT TCTGTGCAAA ACCACAGAGA AAATTGTTCA CCTTTTACCA 
AGCAAGATTC AGAAGACAGA TGTTGGACTG TATGTTGAAG TTGCACAGTG TGTGTCAGAG 
ATGGCAGATA GTGAGGTTGA GCGGATATCT CGCATTTCAA AGGACAACAT AGAGAAAGCT 
ACCTTTTTGC GAGCATATCT GGTCTCACAG GGGCGCTTAC CATTGTCATG CTTGAATGAT 
GTTATTGAAG TGGCAACCAA GTGCAGGGAG AAACAAACCA TCGTATGGAT GCTGCTGCAG 
GTGTTCTACC AAGCCCGTCT CACAAGTCAC CCAAACACAG GAGTTCTGAA AAGGATGGAA 
TGGCTGCTTG ACCTAACTGT TCATATTAGA AATGCAGTCA ATCAGAACAT TTCCGTGAGC 
AGTGTGGATC ACTCAGAGGA AGTAGAGTTC CTGCTGCAGG TATTTGCAGC CTCAGTGGTG 
TCATGGGCTG ATCATGCATC TCCCCTGCTT ATAGGAGTCA GTGCCAACTG GATGGCATGG 
AAGGAGGAAG AACCATGTAG TACATATTTG GGCACGGGTT CAGTGGATGT GGTAACTGTA 
CAGCAGTGTT TAACCATCCT TCCCCACAGT ATGCAACTCT TGGTGGCCAA AGAGCCTTGG 
AAAGAACATG CCCAAAAGAT CATCGATTGG CTAACAAACC TTCTTGAGTG TCCCAAGGAA 
GCCATATTGG AAACATCAAG ATCCATCTTA AAAGCCACGC TGCTAAGCCT AAGGGGCCTT 
CCAGAGTTTA AGAGGAAAGT TGTGTGGACA AGGGCCTACG GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

focad ( XM_031899765.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755625.1 Xenopus tropicalis focadhesin (focad), transcript variant X2, mRNA.

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CloneID	OXa47895
Clone ID Related Accession (Same CDS sequence)	XM_031899765.1
Accession Version	XM_031899765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	focadhesin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC CATTCACTGA AACTAGTGTG CAGCATGCAT GTTTCCCCAA GATGTCAGAA 
GATCTCAAGA AGAGATTAGA ATTTCCAAAC TCATTAATTC AGACACAGGC GGTGACTCAG 
CTCATTGCAT CTGTGTTAAA AGAAAATGTC TCATTGGGGA AGATAAAACA GTCTTCAAAT 
CAGACCCCAG CTCTGAATAT TCTTTGGGAG AAGTGCTGCA GTAATAATGT GGTTGTAAGG 
ACAGCATGTT GTGAAGCATT GGTGCTACTG GTTGAACAGG ATCATGCTGA TTTCGATTAT 
GTTCTTAATG GAATTTTGAA CCTTATACCT TCTGCTGGGA CTGTACAAGG ACTGCTGAAA 
TGTGTATGGC GCCTCCTATA TACTCAAGCA AGGAATGCAG AAAAGGATGG GGAACACAAA 
GATTTGGGCA TTTATAAAAT TTGGAACCCC CCTCATCCAT TCATCAGCAT ACTGGAAAAC 
AGACCAGACT GTTGGCCTGC ACTTCTGCAG CAGATTTCTA CTTTGCCTCA GCTGTGTCAT 
GAAAGCTGTC CTTCAAGCAA TGTTTATTTT ATGGCGTCGT TTTTGAGGTA CTTGTATTGT 
GAACCCTCCC AGCTACAAGA GTACTCTGCC CTTCGAATAG GTCTCAGAAA AGCTTTAGTT 
ATGGTATCGG GGAAGAGTGG GTCCAACAAT CCGTCAAAAC CAGAAGAGCA CATTCTTCGG 
ATTTTCATTG ACATGATGGG CTACATTCAG TTTTTGGATT CAACTCAAGT TACTGAAGCA 
ACTTTGTACA TAAAAGATTT ATGCAGAAGC CTCCACCTGC ATCCAGATTT CTGGAGAAAT 
GAGCTCTCTG CAGTTTGCTT GCAGGCCTTA TGTGCATGTG AGGCAAGCTT AAAAATTGCA 
GGAGACTGCT CTACACTAAT AGAAATATTG GAACAAAATT CAGATCTGCT TGGTGAGAGC 
TTTCCCACAG AACAGGTATT AATTGGACTC AGTTTACTAT TGCTACAATC ACCAGCTAGC 
CAGCAAAAAC TAATCTTAAA GTTGGTAATG AAGATTCTGT CATTCAGAGG GGAAAACAAT 
TTTCCATCAA TCGTCCAAGT GTTAATTATG CCACTCCTCC AAATTTTATC TACCTCAGCG 
CTGGGATATT GTTTCTCTGA TGATGGTGGA AGCTCGAGAG AGCAGCTGGC TACTAGTCTT 
CTGGAAATGG TTCAGAATGC TGACTGTAGT GGGGAAAGGA GACTGAATGA TGTATCACTT 
CCTCTTCCTG TAACAACATG GCATTGTTCA ATGGCTGCTA CATTGAACAT ACTTAACAAG 
TTTCAAGATG ACCATTCTGC AGCACGTGAC TGGCTGTCTT CCATATCTTC GATGCTGGCC 
CCCAATACTC AAGTTCCTGT CAATGTTTCA CTTTTGCTAA GTTTCCTTCT GATTAGAGAA 
TCTGATTATG GCTTGCGTAA TGTTTTGAAA GTCATCACTG ATGTCTCCAA AGCTGATTCA 
TCTCAGGTTC CATATTTAGT TCCAGTTCTA ATGTTGAGAC TTGGACATTC CTTGGCACCA 
GAATCCTCCA GAGACATTCT GTACACTTTG CCTACCCTGG GCACCCACAA GGTATGTGTG 
GCTCAGATCT TGCGAGTCTT CCAAATGTTA GGAAGTACTT CAAAGCTTCA ACCCATTGCG 
CTTCGTCTTA TGACATCTTT GTGGGAGAAA CAGGACCGAA TCTATCCAGA ATTACAGAAG 
ATGATGGCCT TATGTGACAC TCAGTCATTA GCCACTGGGA AAGACACATT GTGGGAAAAG 
ATGTTGGCAA AAGCAGCAAC TGTCAGAGAT ATCTGCAGAC AGAGGCCATA TCAGCATGGG 
GCAGATATGC TGGCTGCTAT AACGCAAGTT TTACATGAAT GCACAAAAGC CGACCAAGCT 
ACTCCGGCTG CTTTAGCATT GCAGGGTCTT CATGCTCTCT GTCAAGCAGA GGTTGTAGAC 
ATTCGCTCTG CCTGGAATGC TCTTTCCTCC AAACTTAGTT GTGAAACACG TCCCCTTGTC 
CTCAAAGTGT TACATGAACT TTTTGCATTG GTCCCTTCTT TGACTGTTGA TACAGAAGAA 
TATCAGAAAT TTAAAACTGA GATTATCGGT ATCCTTTGGA GGCACACTCA AAGTAAGGAT 
CTCAATGTGA TGGTTTCTGC ATACAAATCT TTATGTGAGT TTGAGGCTGA TGAGCACTCC 
ATTCTTCATA TTCCTGAGCA GGCAAGGCCT GAGGTGGCAC CTAAAGATGA ATCTGACCTG 
GCTAGTGAAG AAGAGGAGAA AGAAACTGAC ATTTCTATCC CTGGCTCATC ATATATAAAA 
TTAATGGCTT TGACACCGGT ATCTGTTTTA CCAGCTCTTG AAGCTTTCCT TTCATCTCTT 
GTAAAGCAAG AAATGAGCCG CATGCCACGT GGTATTTATC ATACAGCTCT GCGTGGTGGT 
AATATTAGAT CTGACCAAGG CAAAACTATT GCAGGAGTCC CAAACTTCAT GCTAAAAATG 
TACGAGAAGA ATAAACAGCC TGGCCTGAAA CCTGGCCTTG CAGCTGGCAT GCTGCTGTGT 
TACGATCTCC CAATCCAGAC AGACAAGGAT GGCAGGCCCA TTAATCGTTT CCTTGCCAGT 
AGAGGGCGCA GCTACCAGCA GATGTTAACC GCACTTATCA ATGAGGTGAA TATTCAGTCT 
TCAGAATGGC ATCGCTCTAT ATTGCTGCCC CAGTCATGGC TTGGCTTCAT GACAAGGACA 
TACCATGCTG TTCTGCAAGG AAGGCAAGCT GAACTGGAAA TGCAGCTGAA ACATGGAAAG 
GATGACCCAC AAGAGCTTCA GTACAAAACA TTTACAGCTT GGTTGTGGGT AAGGGATGCA 
TTGACCGATG TCATTAGGTG TGCTGTGAAA GACAGTCCTG TGGTGAAGGG AAATTCTCTG 
TTGGCATTGA CTGGACTTGC TGTGGCTGTA TCAAAATATG AAAGCAGTCT TCCATCAGAA 
ACAGAGGGAG CCCCAGAGAT TGAGCCTGAT ATTCTTCCCA CAAGTGCTTG GATCACCATG 
GTTCTTGACA CCCTTTTGAG TACTGTGGAC AATCATTATC AACCGAAAGG AAGAATCTTC 
CCTTGGTTCC AACATAAATC TTACTCTGGA GAAAACACAG CGAGTGTTAT TGCTAGGTCC 
TGTGCTGCCA CAGCTCTTTC CCTGCTTGTA CCCGTACTTG TTGTTTCTAA TAAAGAGAAG 
GTGGAAGATA TTCTTAACAT CTTGACAGCC AGGCTACCTG GAAAGCCATT AGCAGATGAC 
TCTCAGTCGC TTCAGATACA CATGGGACTT GCGGTTGGCA TGTTCATTTC CAGATTGTTC 
GAGGAGAAAG TCAGTGATGT ATCTGGTCGG CAAATGAATC TACTAATGAT GAGGTCTCTG 
GATGCACTGG AAAGTTGCTG CTTTGACCAA AGCCTGGAAT ACAACATGGG CTGCATTCTG 
GGAGTGGGCC TTGTTTTGTC TTTTATGTGT CAGAGCAGCC AAACTGATAG CCAGGTTCAT 
GTTTCTACTA CTCTGCGCAA ATTTTATATG GCCTTGAGTG AAGACAAGAG CAGAACAGAG 
CAAGAAGGTT TGTCGTACTC TTTGGCCTGT GGGACAGTGT CGGCGTTCAG TGCTGGAATC 
CTTAGTGCTG AAGAAGCTGA AGAGCATATG AACAAGCTGC GTTTGATGAC AGAGCAAAAT 
CAACAGACTC CTGGCTTAGC TCTGGCACTT GGAAGTATTG TCCATGGTTT GTCCATTTGT 
GGACATGGGA AGGCAGAAGA TCTAAATAAC AGATTGCTGC CTGCCTGGAT GAAGATTCTT 
CTTGCAGAGG GTTGCCCCAC AATGCAACGG CTGGCGGCTA TTAATGGCCT TGTGGCACTG 
GTAGGATCAG AATCTGCATT GATACAACTT AAATCTGAAG TAATCCATTC CTCACAGTTT 
CAAAGCAAGC TTAACGAAGT AATTAGAGCC ATAACTCAGA TTGTTAGTTT TTCTGGAGCA 
ATCGGACTGC AGACAAATGC CGCATGGATT CTTGGTCATC TACACTTATC CAGCATTTCA 
TCTTCTCAAA GCAGAACGTC TATTCCTCCA GATTTCAGCT ATCTCCCTGA AAGAAGTCTA 
ATTCGGGCTG TAGTGGACTT TCTTATTTCC GCTGGCAAGA AAGGCCCTGA ATCTCTTCCA 
CCTTGCTTAG TGAAGGTCTG TATATCTTCC ATTGCTGCTG CTGCAGAGGG ACATCAATAT 
CCACCCGTTA ACTGGGCATC CATTCTCGCC CCTCTAATGA GACTCAACTT TGGGGAAGAT 
ACCCAGCAAC TTTGTATTCA AATAGCGGTG ACACAGGCCC AAAGCTCACA GAATGCTGCA 
CTGCTTCTTG GGATGTGGCT GGTGCCCCCA CTGGTTTATA GTCTAACAGT ACAGTGTCGT 
TCCTATCTTT TAAACTCCCT ATCTCTGTGG ATGAAGCATG TATCTGAAGA TAAGCTACAG 
ACCTTCTCTG ACTTATTTAT AGTGACTCAG TTTGAGACCC AAAGCAAGAT GGTGGATTTG 
TGTTGGAGCA TTCTACAGGG ACTTAAGCAA GCTATGAAAC TTCCAAACCC CCCGCAGCAT 
TGCTGGAGCT TTCTGTGCAA AACCACAGAG AAAATTGTTC ACCTTTTACC AAGCAAGATT 
CAGAAGACAG ATGTTGGACT GTATGTTGAA GTTGCACAGT GTGTGTCAGA GATGGCAGAT 
AGTGAGGTTG AGCGGATATC TCGCATTTCA AAGGACAACA TAGAGAAAGC TACCTTTTTG 
CGAGCATATC TGGTCTCACA GGGGCGCTTA CCATTGTCAT GCTTGAATGA TGTTATTGAA 
GTGGCAACCA AGTGCAGGGA GAAACAAACC ATCGTATGGA TGCTGCTGCA GGTGTTCTAC 
CAAGCCCGTC TCACAAGTCA CCCAAACACA GGAGTTCTGA AAAGGATGGA ATGGCTGCTT 
GACCTAACTG TTCATATTAG AAATGCAGTC AATCAGAACA TTTCCGTGAG CAGTGTGGAT 
CACTCAGAGG AGTCAGTGCC AACTGGATGG CATGGAAGGA GGAAGAACCA TGTAGTACAT 
ATTTGGGCAC GGGTTCAGTG GATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755625.1
CDS	89..5218
Translation	MATPFTETSVQHACFPKMSEDLKKRLEFPNSLIQTQAVTQLIASVLKENVSLGKIKQSSNQTPALNILWEKCCSNNVVVRTACCEALVLLVEQDHADFDYVLNGILNLIPSAGTVQGLLKCVWRLLYTQARNAEKDGEHKDLGIYKIWNPPHPFISILENRPDCWPALLQQISTLPQLCHESCPSSNVYFMASFLRYLYCEPSQLQEYSALRIGLRKALVMVSGKSGSNNPSKPEEHILRIFIDMMGYIQFLDSTQVTEATLYIKDLCRSLHLHPDFWRNELSAVCLQALCACEASLKIAGDCSTLIEILEQNSDLLGESFPTEQVLIGLSLLLLQSPASQQKLILKLVMKILSFRGENNFPSIVQVLIMPLLQILSTSALGYCFSDDGGSSREQLATSLLEMVQNADCSGERRLNDVSLPLPVTTWHCSMAATLNILNKFQDDHSAARDWLSSISSMLAPNTQVPVNVSLLLSFLLIRESDYGLRNVLKVITDVSKADSSQVPYLVPVLMLRLGHSLAPESSRDILYTLPTLGTHKVCVAQILRVFQMLGSTSKLQPIALRLMTSLWEKQDRIYPELQKMMALCDTQSLATGKDTLWEKMLAKAATVRDICRQRPYQHGADMLAAITQVLHECTKADQATPAALALQGLHALCQAEVVDIRSAWNALSSKLSCETRPLVLKVLHELFALVPSLTVDTEEYQKFKTEIIGILWRHTQSKDLNVMVSAYKSLCEFEADEHSILHIPEQARPEVAPKDESDLASEEEEKETDISIPGSSYIKLMALTPVSVLPALEAFLSSLVKQEMSRMPRGIYHTALRGGNIRSDQGKTIAGVPNFMLKMYEKNKQPGLKPGLAAGMLLCYDLPIQTDKDGRPINRFLASRGRSYQQMLTALINEVNIQSSEWHRSILLPQSWLGFMTRTYHAVLQGRQAELEMQLKHGKDDPQELQYKTFTAWLWVRDALTDVIRCAVKDSPVVKGNSLLALTGLAVAVSKYESSLPSETEGAPEIEPDILPTSAWITMVLDTLLSTVDNHYQPKGRIFPWFQHKSYSGENTASVIARSCAATALSLLVPVLVVSNKEKVEDILNILTARLPGKPLADDSQSLQIHMGLAVGMFISRLFEEKVSDVSGRQMNLLMMRSLDALESCCFDQSLEYNMGCILGVGLVLSFMCQSSQTDSQVHVSTTLRKFYMALSEDKSRTEQEGLSYSLACGTVSAFSAGILSAEEAEEHMNKLRLMTEQNQQTPGLALALGSIVHGLSICGHGKAEDLNNRLLPAWMKILLAEGCPTMQRLAAINGLVALVGSESALIQLKSEVIHSSQFQSKLNEVIRAITQIVSFSGAIGLQTNAAWILGHLHLSSISSSQSRTSIPPDFSYLPERSLIRAVVDFLISAGKKGPESLPPCLVKVCISSIAAAAEGHQYPPVNWASILAPLMRLNFGEDTQQLCIQIAVTQAQSSQNAALLLGMWLVPPLVYSLTVQCRSYLLNSLSLWMKHVSEDKLQTFSDLFIVTQFETQSKMVDLCWSILQGLKQAMKLPNPPQHCWSFLCKTTEKIVHLLPSKIQKTDVGLYVEVAQCVSEMADSEVERISRISKDNIEKATFLRAYLVSQGRLPLSCLNDVIEVATKCREKQTIVWMLLQVFYQARLTSHPNTGVLKRMEWLLDLTVHIRNAVNQNISVSSVDHSEESVPTGWHGRRKNHVVHIWARVQWMW

Target ORF information:

RefSeq Version	XM_031899765.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis focadhesin (focad), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899765.1

ORF Insert Sequence:

ATGGCCACGC CATTCACTGA AACTAGTGTG CAGCATGCAT GTTTCCCCAA GATGTCAGAA 
GATCTCAAGA AGAGATTAGA ATTTCCAAAC TCATTAATTC AGACACAGGC GGTGACTCAG 
CTCATTGCAT CTGTGTTAAA AGAAAATGTC TCATTGGGGA AGATAAAACA GTCTTCAAAT 
CAGACCCCAG CTCTGAATAT TCTTTGGGAG AAGTGCTGCA GTAATAATGT GGTTGTAAGG 
ACAGCATGTT GTGAAGCATT GGTGCTACTG GTTGAACAGG ATCATGCTGA TTTCGATTAT 
GTTCTTAATG GAATTTTGAA CCTTATACCT TCTGCTGGGA CTGTACAAGG ACTGCTGAAA 
TGTGTATGGC GCCTCCTATA TACTCAAGCA AGGAATGCAG AAAAGGATGG GGAACACAAA 
GATTTGGGCA TTTATAAAAT TTGGAACCCC CCTCATCCAT TCATCAGCAT ACTGGAAAAC 
AGACCAGACT GTTGGCCTGC ACTTCTGCAG CAGATTTCTA CTTTGCCTCA GCTGTGTCAT 
GAAAGCTGTC CTTCAAGCAA TGTTTATTTT ATGGCGTCGT TTTTGAGGTA CTTGTATTGT 
GAACCCTCCC AGCTACAAGA GTACTCTGCC CTTCGAATAG GTCTCAGAAA AGCTTTAGTT 
ATGGTATCGG GGAAGAGTGG GTCCAACAAT CCGTCAAAAC CAGAAGAGCA CATTCTTCGG 
ATTTTCATTG ACATGATGGG CTACATTCAG TTTTTGGATT CAACTCAAGT TACTGAAGCA 
ACTTTGTACA TAAAAGATTT ATGCAGAAGC CTCCACCTGC ATCCAGATTT CTGGAGAAAT 
GAGCTCTCTG CAGTTTGCTT GCAGGCCTTA TGTGCATGTG AGGCAAGCTT AAAAATTGCA 
GGAGACTGCT CTACACTAAT AGAAATATTG GAACAAAATT CAGATCTGCT TGGTGAGAGC 
TTTCCCACAG AACAGGTATT AATTGGACTC AGTTTACTAT TGCTACAATC ACCAGCTAGC 
CAGCAAAAAC TAATCTTAAA GTTGGTAATG AAGATTCTGT CATTCAGAGG GGAAAACAAT 
TTTCCATCAA TCGTCCAAGT GTTAATTATG CCACTCCTCC AAATTTTATC TACCTCAGCG 
CTGGGATATT GTTTCTCTGA TGATGGTGGA AGCTCGAGAG AGCAGCTGGC TACTAGTCTT 
CTGGAAATGG TTCAGAATGC TGACTGTAGT GGGGAAAGGA GACTGAATGA TGTATCACTT 
CCTCTTCCTG TAACAACATG GCATTGTTCA ATGGCTGCTA CATTGAACAT ACTTAACAAG 
TTTCAAGATG ACCATTCTGC AGCACGTGAC TGGCTGTCTT CCATATCTTC GATGCTGGCC 
CCCAATACTC AAGTTCCTGT CAATGTTTCA CTTTTGCTAA GTTTCCTTCT GATTAGAGAA 
TCTGATTATG GCTTGCGTAA TGTTTTGAAA GTCATCACTG ATGTCTCCAA AGCTGATTCA 
TCTCAGGTTC CATATTTAGT TCCAGTTCTA ATGTTGAGAC TTGGACATTC CTTGGCACCA 
GAATCCTCCA GAGACATTCT GTACACTTTG CCTACCCTGG GCACCCACAA GGTATGTGTG 
GCTCAGATCT TGCGAGTCTT CCAAATGTTA GGAAGTACTT CAAAGCTTCA ACCCATTGCG 
CTTCGTCTTA TGACATCTTT GTGGGAGAAA CAGGACCGAA TCTATCCAGA ATTACAGAAG 
ATGATGGCCT TATGTGACAC TCAGTCATTA GCCACTGGGA AAGACACATT GTGGGAAAAG 
ATGTTGGCAA AAGCAGCAAC TGTCAGAGAT ATCTGCAGAC AGAGGCCATA TCAGCATGGG 
GCAGATATGC TGGCTGCTAT AACGCAAGTT TTACATGAAT GCACAAAAGC CGACCAAGCT 
ACTCCGGCTG CTTTAGCATT GCAGGGTCTT CATGCTCTCT GTCAAGCAGA GGTTGTAGAC 
ATTCGCTCTG CCTGGAATGC TCTTTCCTCC AAACTTAGTT GTGAAACACG TCCCCTTGTC 
CTCAAAGTGT TACATGAACT TTTTGCATTG GTCCCTTCTT TGACTGTTGA TACAGAAGAA 
TATCAGAAAT TTAAAACTGA GATTATCGGT ATCCTTTGGA GGCACACTCA AAGTAAGGAT 
CTCAATGTGA TGGTTTCTGC ATACAAATCT TTATGTGAGT TTGAGGCTGA TGAGCACTCC 
ATTCTTCATA TTCCTGAGCA GGCAAGGCCT GAGGTGGCAC CTAAAGATGA ATCTGACCTG 
GCTAGTGAAG AAGAGGAGAA AGAAACTGAC ATTTCTATCC CTGGCTCATC ATATATAAAA 
TTAATGGCTT TGACACCGGT ATCTGTTTTA CCAGCTCTTG AAGCTTTCCT TTCATCTCTT 
GTAAAGCAAG AAATGAGCCG CATGCCACGT GGTATTTATC ATACAGCTCT GCGTGGTGGT 
AATATTAGAT CTGACCAAGG CAAAACTATT GCAGGAGTCC CAAACTTCAT GCTAAAAATG 
TACGAGAAGA ATAAACAGCC TGGCCTGAAA CCTGGCCTTG CAGCTGGCAT GCTGCTGTGT 
TACGATCTCC CAATCCAGAC AGACAAGGAT GGCAGGCCCA TTAATCGTTT CCTTGCCAGT 
AGAGGGCGCA GCTACCAGCA GATGTTAACC GCACTTATCA ATGAGGTGAA TATTCAGTCT 
TCAGAATGGC ATCGCTCTAT ATTGCTGCCC CAGTCATGGC TTGGCTTCAT GACAAGGACA 
TACCATGCTG TTCTGCAAGG AAGGCAAGCT GAACTGGAAA TGCAGCTGAA ACATGGAAAG 
GATGACCCAC AAGAGCTTCA GTACAAAACA TTTACAGCTT GGTTGTGGGT AAGGGATGCA 
TTGACCGATG TCATTAGGTG TGCTGTGAAA GACAGTCCTG TGGTGAAGGG AAATTCTCTG 
TTGGCATTGA CTGGACTTGC TGTGGCTGTA TCAAAATATG AAAGCAGTCT TCCATCAGAA 
ACAGAGGGAG CCCCAGAGAT TGAGCCTGAT ATTCTTCCCA CAAGTGCTTG GATCACCATG 
GTTCTTGACA CCCTTTTGAG TACTGTGGAC AATCATTATC AACCGAAAGG AAGAATCTTC 
CCTTGGTTCC AACATAAATC TTACTCTGGA GAAAACACAG CGAGTGTTAT TGCTAGGTCC 
TGTGCTGCCA CAGCTCTTTC CCTGCTTGTA CCCGTACTTG TTGTTTCTAA TAAAGAGAAG 
GTGGAAGATA TTCTTAACAT CTTGACAGCC AGGCTACCTG GAAAGCCATT AGCAGATGAC 
TCTCAGTCGC TTCAGATACA CATGGGACTT GCGGTTGGCA TGTTCATTTC CAGATTGTTC 
GAGGAGAAAG TCAGTGATGT ATCTGGTCGG CAAATGAATC TACTAATGAT GAGGTCTCTG 
GATGCACTGG AAAGTTGCTG CTTTGACCAA AGCCTGGAAT ACAACATGGG CTGCATTCTG 
GGAGTGGGCC TTGTTTTGTC TTTTATGTGT CAGAGCAGCC AAACTGATAG CCAGGTTCAT 
GTTTCTACTA CTCTGCGCAA ATTTTATATG GCCTTGAGTG AAGACAAGAG CAGAACAGAG 
CAAGAAGGTT TGTCGTACTC TTTGGCCTGT GGGACAGTGT CGGCGTTCAG TGCTGGAATC 
CTTAGTGCTG AAGAAGCTGA AGAGCATATG AACAAGCTGC GTTTGATGAC AGAGCAAAAT 
CAACAGACTC CTGGCTTAGC TCTGGCACTT GGAAGTATTG TCCATGGTTT GTCCATTTGT 
GGACATGGGA AGGCAGAAGA TCTAAATAAC AGATTGCTGC CTGCCTGGAT GAAGATTCTT 
CTTGCAGAGG GTTGCCCCAC AATGCAACGG CTGGCGGCTA TTAATGGCCT TGTGGCACTG 
GTAGGATCAG AATCTGCATT GATACAACTT AAATCTGAAG TAATCCATTC CTCACAGTTT 
CAAAGCAAGC TTAACGAAGT AATTAGAGCC ATAACTCAGA TTGTTAGTTT TTCTGGAGCA 
ATCGGACTGC AGACAAATGC CGCATGGATT CTTGGTCATC TACACTTATC CAGCATTTCA 
TCTTCTCAAA GCAGAACGTC TATTCCTCCA GATTTCAGCT ATCTCCCTGA AAGAAGTCTA 
ATTCGGGCTG TAGTGGACTT TCTTATTTCC GCTGGCAAGA AAGGCCCTGA ATCTCTTCCA 
CCTTGCTTAG TGAAGGTCTG TATATCTTCC ATTGCTGCTG CTGCAGAGGG ACATCAATAT 
CCACCCGTTA ACTGGGCATC CATTCTCGCC CCTCTAATGA GACTCAACTT TGGGGAAGAT 
ACCCAGCAAC TTTGTATTCA AATAGCGGTG ACACAGGCCC AAAGCTCACA GAATGCTGCA 
CTGCTTCTTG GGATGTGGCT GGTGCCCCCA CTGGTTTATA GTCTAACAGT ACAGTGTCGT 
TCCTATCTTT TAAACTCCCT ATCTCTGTGG ATGAAGCATG TATCTGAAGA TAAGCTACAG 
ACCTTCTCTG ACTTATTTAT AGTGACTCAG TTTGAGACCC AAAGCAAGAT GGTGGATTTG 
TGTTGGAGCA TTCTACAGGG ACTTAAGCAA GCTATGAAAC TTCCAAACCC CCCGCAGCAT 
TGCTGGAGCT TTCTGTGCAA AACCACAGAG AAAATTGTTC ACCTTTTACC AAGCAAGATT 
CAGAAGACAG ATGTTGGACT GTATGTTGAA GTTGCACAGT GTGTGTCAGA GATGGCAGAT 
AGTGAGGTTG AGCGGATATC TCGCATTTCA AAGGACAACA TAGAGAAAGC TACCTTTTTG 
CGAGCATATC TGGTCTCACA GGGGCGCTTA CCATTGTCAT GCTTGAATGA TGTTATTGAA 
GTGGCAACCA AGTGCAGGGA GAAACAAACC ATCGTATGGA TGCTGCTGCA GGTGTTCTAC 
CAAGCCCGTC TCACAAGTCA CCCAAACACA GGAGTTCTGA AAAGGATGGA ATGGCTGCTT 
GACCTAACTG TTCATATTAG AAATGCAGTC AATCAGAACA TTTCCGTGAG CAGTGTGGAT 
CACTCAGAGG AGTCAGTGCC AACTGGATGG CATGGAAGGA GGAAGAACCA TGTAGTACAT 
ATTTGGGCAC GGGTTCAGTG GATGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

focad ( XM_012962106.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817560.1 Xenopus tropicalis focadhesin (focad), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa27621
Clone ID Related Accession (Same CDS sequence)	NM_001374673.1 , XM_012962106.2
Accession Version	XM_012962106.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	focadhesin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC CATTCACTGA AACTAGTGTG CAGCATGCAT GTTTCCCCAA GATGTCAGAA 
GATCTCAAGA AGAGATTAGA ATTTCCAAAC TCATTAATTC AGACACAGGC GGTGACTCAG 
CTCATTGCAT CTGTGTTAAA AGAAAATGTC TCATTGGGGA AGATAAAACA GTCTTCAAAT 
CAGACCCCAG CTCTGAATAT TCTTTGGGAG AAGTGCTGCA GTAATAATGT GGTTGTAAGG 
ACAGCATGTT GTGAAGCATT GGTGCTACTG GTTGAACAGG ATCATGCTGA TTTCGATTAT 
GTTCTTAATG GAATTTTGAA CCTTATACCT TCTGCTGGGA CTGTACAAGG ACTGCTGAAA 
TGTGTATGGC GCCTCCTATA TACTCAAGCA AGGAATGCAG AAAAGGATGG GGAACACAAA 
GATTTGGGCA TTTATAAAAT TTGGAACCCC CCTCATCCAT TCATCAGCAT ACTGGAAAAC 
AGACCAGACT GTTGGCCTGC ACTTCTGCAG CAGATTTCTA CTTTGCCTCA GCTGTGTCAT 
GAAAGCTGTC CTTCAAGCAA TGTTTATTTT ATGGCGTCGT TTTTGAGGTA CTTGTATTGT 
GAACCCTCCC AGCTACAAGA GTACTCTGCC CTTCGAATAG GTCTCAGAAA AGCTTTAGTT 
ATGGTATCGG GGAAGAGTGG GTCCAACAAT CCGTCAAAAC CAGAAGAGCA CATTCTTCGG 
ATTTTCATTG ACATGATGGG CTACATTCAG TTTTTGGATT CAACTCAAGT TACTGAAGCA 
ACTTTGTACA TAAAAGATTT ATGCAGAAGC CTCCACCTGC ATCCAGATTT CTGGAGAAAT 
GAGCTCTCTG CAGTTTGCTT GCAGGCCTTA TGTGCATGTG AGGCAAGCTT AAAAATTGCA 
GGAGACTGCT CTACACTAAT AGAAATATTG GAACAAAATT CAGATCTGCT TGGTGAGAGC 
TTTCCCACAG AACAGGTATT AATTGGACTC AGTTTACTAT TGCTACAATC ACCAGCTAGC 
CAGCAAAAAC TAATCTTAAA GTTGGTAATG AAGATTCTGT CATTCAGAGG GGAAAACAAT 
TTTCCATCAA TCGTCCAAGT GTTAATTATG CCACTCCTCC AAATTTTATC TACCTCAGCG 
CTGGGATATT GTTTCTCTGA TGATGGTGGA AGCTCGAGAG AGCAGCTGGC TACTAGTCTT 
CTGGAAATGG TTCAGAATGC TGACTGTAGT GGGGAAAGGA GACTGAATGA TGTATCACTT 
CCTCTTCCTG TAACAACATG GCATTGTTCA ATGGCTGCTA CATTGAACAT ACTTAACAAG 
TTTCAAGATG ACCATTCTGC AGCACGTGAC TGGCTGTCTT CCATATCTTC GATGCTGGCC 
CCCAATACTC AAGTTCCTGT CAATGTTTCA CTTTTGCTAA GTTTCCTTCT GATTAGAGAA 
TCTGATTATG GCTTGCGTAA TGTTTTGAAA GTCATCACTG ATGTCTCCAA AGCTGATTCA 
TCTCAGGTTC CATATTTAGT TCCAGTTCTA ATGTTGAGAC TTGGACATTC CTTGGCACCA 
GAATCCTCCA GAGACATTCT GTACACTTTG CCTACCCTGG GCACCCACAA GGTATGTGTG 
GCTCAGATCT TGCGAGTCTT CCAAATGTTA GGAAGTACTT CAAAGCTTCA ACCCATTGCG 
CTTCGTCTTA TGACATCTTT GTGGGAGAAA CAGGACCGAA TCTATCCAGA ATTACAGAAG 
ATGATGGCCT TATGTGACAC TCAGTCATTA GCCACTGGGA AAGACACATT GTGGGAAAAG 
ATGTTGGCAA AAGCAGCAAC TGTCAGAGAT ATCTGCAGAC AGAGGCCATA TCAGCATGGG 
GCAGATATGC TGGCTGCTAT AACGCAAGTT TTACATGAAT GCACAAAAGC CGACCAAGCT 
ACTCCGGCTG CTTTAGCATT GCAGGGTCTT CATGCTCTCT GTCAAGCAGA GGTTGTAGAC 
ATTCGCTCTG CCTGGAATGC TCTTTCCTCC AAACTTAGTT GTGAAACACG TCCCCTTGTC 
CTCAAAGTGT TACATGAACT TTTTGCATTG GTCCCTTCTT TGACTGTTGA TACAGAAGAA 
TATCAGAAAT TTAAAACTGA GATTATCGGT ATCCTTTGGA GGCACACTCA AAGTAAGGAT 
CTCAATGTGA TGGTTTCTGC ATACAAATCT TTATGTGAGT TTGAGGCTGA TGAGCACTCC 
ATTCTTCATA TTCCTGAGCA GGCAAGGCCT GAGGTGGCAC CTAAAGATGA ATCTGACCTG 
GCTAGTGAAG AAGAGGAGAA AGAAACTGAC ATTTCTATCC CTGGCTCATC ATATATAAAA 
TTAATGGCTT TGACACCGGT ATCTGTTTTA CCAGCTCTTG AAGCTTTCCT TTCATCTCTT 
GTAAAGCAAG AAATGAGCCG CATGCCACGT GGTATTTATC ATACAGCTCT GCGTGGTGGT 
AATATTAGAT CTGACCAAGG CAAAACTATT GCAGGAGTCC CAAACTTCAT GCTAAAAATG 
TACGAGAAGA ATAAACAGCC TGGCCTGAAA CCTGGCCTTG CAGCTGGCAT GCTGCTGTGT 
TACGATCTCC CAATCCAGAC AGACAAGGAT GGCAGGCCCA TTAATCGTTT CCTTGCCAGT 
AGAGGGCGCA GCTACCAGCA GATGTTAACC GCACTTATCA ATGAGGTGAA TATTCAGTCT 
TCAGAATGGC ATCGCTCTAT ATTGCTGCCC CAGTCATGGC TTGGCTTCAT GACAAGGACA 
TACCATGCTG TTCTGCAAGG AAGGCAAGCT GAACTGGAAA TGCAGCTGAA ACATGGAAAG 
GATGACCCAC AAGAGCTTCA GTACAAAACA TTTACAGCTT GGTTGTGGGT AAGGGATGCA 
TTGACCGATG TCATTAGGTG TGCTGTGAAA GACAGTCCTG TGGTGAAGGG AAATTCTCTG 
TTGGCATTGA CTGGACTTGC TGTGGCTGTA TCAAAATATG AAAGCAGTCT TCCATCAGAA 
ACAGAGGGAG CCCCAGAGAT TGAGCCTGAT ATTCTTCCCA CAAGTGCTTG GATCACCATG 
GTTCTTGACA CCCTTTTGAG TACTGTGGAC AATCATTATC AACCGAAAGG AAGAATCTTC 
CCTTGGTTCC AACATAAATC TTACTCTGGA GAAAACACAG CGAGTGTTAT TGCTAGGTCC 
TGTGCTGCCA CAGCTCTTTC CCTGCTTGTA CCCGTACTTG TTGTTTCTAA TAAAGAGAAG 
GTGGAAGATA TTCTTAACAT CTTGACAGCC AGGCTACCTG GAAAGCCATT AGCAGATGAC 
TCTCAGTCGC TTCAGATACA CATGGGACTT GCGGTTGGCA TGTTCATTTC CAGATTGTTC 
GAGGAGAAAG TCAGTGATGT ATCTGGTCGG CAAATGAATC TACTAATGAT GAGGTCTCTG 
GATGCACTGG AAAGTTGCTG CTTTGACCAA AGCCTGGAAT ACAACATGGG CTGCATTCTG 
GGAGTGGGCC TTGTTTTGTC TTTTATGTGT CAGAGCAGCC AAACTGATAG CCAGGTTCAT 
GTTTCTACTA CTCTGCGCAA ATTTTATATG GCCTTGAGTG AAGACAAGAG CAGAACAGAG 
CAAGAAGGTT TGTCGTACTC TTTGGCCTGT GGGACAGTGT CGGCGTTCAG TGCTGGAATC 
CTTAGTGCTG AAGAAGCTGA AGAGCATATG AACAAGCTGC GTTTGATGAC AGAGCAAAAT 
CAACAGACTC CTGGCTTAGC TCTGGCACTT GGAAGTATTG TCCATGGTTT GTCCATTTGT 
GGACATGGGA AGGCAGAAGA TCTAAATAAC AGATTGCTGC CTGCCTGGAT GAAGATTCTT 
CTTGCAGAGG GTTGCCCCAC AATGCAACGG CTGGCGGCTA TTAATGGCCT TGTGGCACTG 
GTAGGATCAG AATCTGCATT GATACAACTT AAATCTGAAG TAATCCATTC CTCACAGTTT 
CAAAGCAAGC TTAACGAAGT AATTAGAGCC ATAACTCAGA TTGTTAGTTT TTCTGGAGCA 
ATCGGACTGC AGACAAATGC CGCATGGATT CTTGGTCATC TACACTTATC CAGCATTTCA 
TCTTCTCAAA GCAGAACGTC TATTCCTCCA GATTTCAGCT ATCTCCCTGA AAGAAGTCTA 
ATTCGGGCTG TAGTGGACTT TCTTATTTCC GCTGGCAAGA AAGGCCCTGA ATCTCTTCCA 
CCTTGCTTAG TGAAGGTCTG TATATCTTCC ATTGCTGCTG CTGCAGAGGG ACATCAATAT 
CCACCCGTTA ACTGGGCATC CATTCTCGCC CCTCTAATGA GACTCAACTT TGGGGAAGAT 
ACCCAGCAAC TTTGTATTCA AATAGCGGTG ACACAGGCCC AAAGCTCACA GAATGCTGCA 
CTGCTTCTTG GGATGTGGCT GGTGCCCCCA CTGGTTTATA GTCTAACAGT ACAGTGTCGT 
TCCTATCTTT TAAACTCCCT ATCTCTGTGG ATGAAGCATG TATCTGAAGA TAAGCTACAG 
ACCTTCTCTG ACTTATTTAT AGTGACTCAG TTTGAGACCC AAAGCAAGAT GGTGGATTTG 
TGTTGGAGCA TTCTACAGGG ACTTAAGCAA GCTATGAAAC TTCCAAACCC CCCGCAGCAT 
TGCTGGAGCT TTCTGTGCAA AACCACAGAG AAAATTGTTC ACCTTTTACC AAGCAAGATT 
CAGAAGACAG ATGTTGGACT GTATGTTGAA GTTGCACAGT GTGTGTCAGA GATGGCAGAT 
AGTGAGGTTG AGCGGATATC TCGCATTTCA AAGGACAACA TAGAGAAAGC TACCTTTTTG 
CGAGCATATC TGGTCTCACA GGGGCGCTTA CCATTGTCAT GCTTGAATGA TGTTATTGAA 
GTGGCAACCA AGTGCAGGGA GAAACAAACC ATCGTATGGA TGCTGCTGCA GGTGTTCTAC 
CAAGCCCGTC TCACAAGTCA CCCAAACACA GGAGTTCTGA AAAGGATGGA ATGGCTGCTT 
GACCTAACTG TTCATATTAG AAATGCAGTC AATCAGAACA TTTCCGTGAG CAGTGTGGAT 
CACTCAGAGG AAGTAGAGTT CCTGCTGCAG GTATTTGCAG CCTCAGTGGT GTCATGGGCT 
GATCATGCAT CTCCCCTGCT TATAGGAGTC AGTGCCAACT GGATGGCATG GAAGGAGGAA 
GAACCATGTA GTACATATTT GGGCACGGGT TCAGTGGATG TGGTAACTGT ACAGCAGTGT 
TTAACCATCC TTCCCCACAG TATGCAACTC TTGGTGGCCA AAGAGCCTTG GAAAGAACAT 
GCCCAAAAGA TCATCGATTG GCTAACAAAC CTTCTTGAGT GTCCCAAGGA AGCCATATTG 
GAAACATCAA GATCCATCTT AAAAGCCACG CTGCTAAGCC TAAGGGGCCT TCCAGAGTTT 
AAGAGGAAAG TTGTGTGGAC AAGGGCCTAC GGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817560.1
CDS	117..5555
Translation	MATPFTETSVQHACFPKMSEDLKKRLEFPNSLIQTQAVTQLIASVLKENVSLGKIKQSSNQTPALNILWEKCCSNNVVVRTACCEALVLLVEQDHADFDYVLNGILNLIPSAGTVQGLLKCVWRLLYTQARNAEKDGEHKDLGIYKIWNPPHPFISILENRPDCWPALLQQISTLPQLCHESCPSSNVYFMASFLRYLYCEPSQLQEYSALRIGLRKALVMVSGKSGSNNPSKPEEHILRIFIDMMGYIQFLDSTQVTEATLYIKDLCRSLHLHPDFWRNELSAVCLQALCACEASLKIAGDCSTLIEILEQNSDLLGESFPTEQVLIGLSLLLLQSPASQQKLILKLVMKILSFRGENNFPSIVQVLIMPLLQILSTSALGYCFSDDGGSSREQLATSLLEMVQNADCSGERRLNDVSLPLPVTTWHCSMAATLNILNKFQDDHSAARDWLSSISSMLAPNTQVPVNVSLLLSFLLIRESDYGLRNVLKVITDVSKADSSQVPYLVPVLMLRLGHSLAPESSRDILYTLPTLGTHKVCVAQILRVFQMLGSTSKLQPIALRLMTSLWEKQDRIYPELQKMMALCDTQSLATGKDTLWEKMLAKAATVRDICRQRPYQHGADMLAAITQVLHECTKADQATPAALALQGLHALCQAEVVDIRSAWNALSSKLSCETRPLVLKVLHELFALVPSLTVDTEEYQKFKTEIIGILWRHTQSKDLNVMVSAYKSLCEFEADEHSILHIPEQARPEVAPKDESDLASEEEEKETDISIPGSSYIKLMALTPVSVLPALEAFLSSLVKQEMSRMPRGIYHTALRGGNIRSDQGKTIAGVPNFMLKMYEKNKQPGLKPGLAAGMLLCYDLPIQTDKDGRPINRFLASRGRSYQQMLTALINEVNIQSSEWHRSILLPQSWLGFMTRTYHAVLQGRQAELEMQLKHGKDDPQELQYKTFTAWLWVRDALTDVIRCAVKDSPVVKGNSLLALTGLAVAVSKYESSLPSETEGAPEIEPDILPTSAWITMVLDTLLSTVDNHYQPKGRIFPWFQHKSYSGENTASVIARSCAATALSLLVPVLVVSNKEKVEDILNILTARLPGKPLADDSQSLQIHMGLAVGMFISRLFEEKVSDVSGRQMNLLMMRSLDALESCCFDQSLEYNMGCILGVGLVLSFMCQSSQTDSQVHVSTTLRKFYMALSEDKSRTEQEGLSYSLACGTVSAFSAGILSAEEAEEHMNKLRLMTEQNQQTPGLALALGSIVHGLSICGHGKAEDLNNRLLPAWMKILLAEGCPTMQRLAAINGLVALVGSESALIQLKSEVIHSSQFQSKLNEVIRAITQIVSFSGAIGLQTNAAWILGHLHLSSISSSQSRTSIPPDFSYLPERSLIRAVVDFLISAGKKGPESLPPCLVKVCISSIAAAAEGHQYPPVNWASILAPLMRLNFGEDTQQLCIQIAVTQAQSSQNAALLLGMWLVPPLVYSLTVQCRSYLLNSLSLWMKHVSEDKLQTFSDLFIVTQFETQSKMVDLCWSILQGLKQAMKLPNPPQHCWSFLCKTTEKIVHLLPSKIQKTDVGLYVEVAQCVSEMADSEVERISRISKDNIEKATFLRAYLVSQGRLPLSCLNDVIEVATKCREKQTIVWMLLQVFYQARLTSHPNTGVLKRMEWLLDLTVHIRNAVNQNISVSSVDHSEEVEFLLQVFAASVVSWADHASPLLIGVSANWMAWKEEEPCSTYLGTGSVDVVTVQQCLTILPHSMQLLVAKEPWKEHAQKIIDWLTNLLECPKEAILETSRSILKATLLSLRGLPEFKRKVVWTRAYGW

Target ORF information:

RefSeq Version	XM_012962106.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis focadhesin (focad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962106.2

ORF Insert Sequence:

ATGGCCACGC CATTCACTGA AACTAGTGTG CAGCATGCAT GTTTCCCCAA GATGTCAGAA 
GATCTCAAGA AGAGATTAGA ATTTCCAAAC TCATTAATTC AGACACAGGC GGTGACTCAG 
CTCATTGCAT CTGTGTTAAA AGAAAATGTC TCATTGGGGA AGATAAAACA GTCTTCAAAT 
CAGACCCCAG CTCTGAATAT TCTTTGGGAG AAGTGCTGCA GTAATAATGT GGTTGTAAGG 
ACAGCATGTT GTGAAGCATT GGTGCTACTG GTTGAACAGG ATCATGCTGA TTTCGATTAT 
GTTCTTAATG GAATTTTGAA CCTTATACCT TCTGCTGGGA CTGTACAAGG ACTGCTGAAA 
TGTGTATGGC GCCTCCTATA TACTCAAGCA AGGAATGCAG AAAAGGATGG GGAACACAAA 
GATTTGGGCA TTTATAAAAT TTGGAACCCC CCTCATCCAT TCATCAGCAT ACTGGAAAAC 
AGACCAGACT GTTGGCCTGC ACTTCTGCAG CAGATTTCTA CTTTGCCTCA GCTGTGTCAT 
GAAAGCTGTC CTTCAAGCAA TGTTTATTTT ATGGCGTCGT TTTTGAGGTA CTTGTATTGT 
GAACCCTCCC AGCTACAAGA GTACTCTGCC CTTCGAATAG GTCTCAGAAA AGCTTTAGTT 
ATGGTATCGG GGAAGAGTGG GTCCAACAAT CCGTCAAAAC CAGAAGAGCA CATTCTTCGG 
ATTTTCATTG ACATGATGGG CTACATTCAG TTTTTGGATT CAACTCAAGT TACTGAAGCA 
ACTTTGTACA TAAAAGATTT ATGCAGAAGC CTCCACCTGC ATCCAGATTT CTGGAGAAAT 
GAGCTCTCTG CAGTTTGCTT GCAGGCCTTA TGTGCATGTG AGGCAAGCTT AAAAATTGCA 
GGAGACTGCT CTACACTAAT AGAAATATTG GAACAAAATT CAGATCTGCT TGGTGAGAGC 
TTTCCCACAG AACAGGTATT AATTGGACTC AGTTTACTAT TGCTACAATC ACCAGCTAGC 
CAGCAAAAAC TAATCTTAAA GTTGGTAATG AAGATTCTGT CATTCAGAGG GGAAAACAAT 
TTTCCATCAA TCGTCCAAGT GTTAATTATG CCACTCCTCC AAATTTTATC TACCTCAGCG 
CTGGGATATT GTTTCTCTGA TGATGGTGGA AGCTCGAGAG AGCAGCTGGC TACTAGTCTT 
CTGGAAATGG TTCAGAATGC TGACTGTAGT GGGGAAAGGA GACTGAATGA TGTATCACTT 
CCTCTTCCTG TAACAACATG GCATTGTTCA ATGGCTGCTA CATTGAACAT ACTTAACAAG 
TTTCAAGATG ACCATTCTGC AGCACGTGAC TGGCTGTCTT CCATATCTTC GATGCTGGCC 
CCCAATACTC AAGTTCCTGT CAATGTTTCA CTTTTGCTAA GTTTCCTTCT GATTAGAGAA 
TCTGATTATG GCTTGCGTAA TGTTTTGAAA GTCATCACTG ATGTCTCCAA AGCTGATTCA 
TCTCAGGTTC CATATTTAGT TCCAGTTCTA ATGTTGAGAC TTGGACATTC CTTGGCACCA 
GAATCCTCCA GAGACATTCT GTACACTTTG CCTACCCTGG GCACCCACAA GGTATGTGTG 
GCTCAGATCT TGCGAGTCTT CCAAATGTTA GGAAGTACTT CAAAGCTTCA ACCCATTGCG 
CTTCGTCTTA TGACATCTTT GTGGGAGAAA CAGGACCGAA TCTATCCAGA ATTACAGAAG 
ATGATGGCCT TATGTGACAC TCAGTCATTA GCCACTGGGA AAGACACATT GTGGGAAAAG 
ATGTTGGCAA AAGCAGCAAC TGTCAGAGAT ATCTGCAGAC AGAGGCCATA TCAGCATGGG 
GCAGATATGC TGGCTGCTAT AACGCAAGTT TTACATGAAT GCACAAAAGC CGACCAAGCT 
ACTCCGGCTG CTTTAGCATT GCAGGGTCTT CATGCTCTCT GTCAAGCAGA GGTTGTAGAC 
ATTCGCTCTG CCTGGAATGC TCTTTCCTCC AAACTTAGTT GTGAAACACG TCCCCTTGTC 
CTCAAAGTGT TACATGAACT TTTTGCATTG GTCCCTTCTT TGACTGTTGA TACAGAAGAA 
TATCAGAAAT TTAAAACTGA GATTATCGGT ATCCTTTGGA GGCACACTCA AAGTAAGGAT 
CTCAATGTGA TGGTTTCTGC ATACAAATCT TTATGTGAGT TTGAGGCTGA TGAGCACTCC 
ATTCTTCATA TTCCTGAGCA GGCAAGGCCT GAGGTGGCAC CTAAAGATGA ATCTGACCTG 
GCTAGTGAAG AAGAGGAGAA AGAAACTGAC ATTTCTATCC CTGGCTCATC ATATATAAAA 
TTAATGGCTT TGACACCGGT ATCTGTTTTA CCAGCTCTTG AAGCTTTCCT TTCATCTCTT 
GTAAAGCAAG AAATGAGCCG CATGCCACGT GGTATTTATC ATACAGCTCT GCGTGGTGGT 
AATATTAGAT CTGACCAAGG CAAAACTATT GCAGGAGTCC CAAACTTCAT GCTAAAAATG 
TACGAGAAGA ATAAACAGCC TGGCCTGAAA CCTGGCCTTG CAGCTGGCAT GCTGCTGTGT 
TACGATCTCC CAATCCAGAC AGACAAGGAT GGCAGGCCCA TTAATCGTTT CCTTGCCAGT 
AGAGGGCGCA GCTACCAGCA GATGTTAACC GCACTTATCA ATGAGGTGAA TATTCAGTCT 
TCAGAATGGC ATCGCTCTAT ATTGCTGCCC CAGTCATGGC TTGGCTTCAT GACAAGGACA 
TACCATGCTG TTCTGCAAGG AAGGCAAGCT GAACTGGAAA TGCAGCTGAA ACATGGAAAG 
GATGACCCAC AAGAGCTTCA GTACAAAACA TTTACAGCTT GGTTGTGGGT AAGGGATGCA 
TTGACCGATG TCATTAGGTG TGCTGTGAAA GACAGTCCTG TGGTGAAGGG AAATTCTCTG 
TTGGCATTGA CTGGACTTGC TGTGGCTGTA TCAAAATATG AAAGCAGTCT TCCATCAGAA 
ACAGAGGGAG CCCCAGAGAT TGAGCCTGAT ATTCTTCCCA CAAGTGCTTG GATCACCATG 
GTTCTTGACA CCCTTTTGAG TACTGTGGAC AATCATTATC AACCGAAAGG AAGAATCTTC 
CCTTGGTTCC AACATAAATC TTACTCTGGA GAAAACACAG CGAGTGTTAT TGCTAGGTCC 
TGTGCTGCCA CAGCTCTTTC CCTGCTTGTA CCCGTACTTG TTGTTTCTAA TAAAGAGAAG 
GTGGAAGATA TTCTTAACAT CTTGACAGCC AGGCTACCTG GAAAGCCATT AGCAGATGAC 
TCTCAGTCGC TTCAGATACA CATGGGACTT GCGGTTGGCA TGTTCATTTC CAGATTGTTC 
GAGGAGAAAG TCAGTGATGT ATCTGGTCGG CAAATGAATC TACTAATGAT GAGGTCTCTG 
GATGCACTGG AAAGTTGCTG CTTTGACCAA AGCCTGGAAT ACAACATGGG CTGCATTCTG 
GGAGTGGGCC TTGTTTTGTC TTTTATGTGT CAGAGCAGCC AAACTGATAG CCAGGTTCAT 
GTTTCTACTA CTCTGCGCAA ATTTTATATG GCCTTGAGTG AAGACAAGAG CAGAACAGAG 
CAAGAAGGTT TGTCGTACTC TTTGGCCTGT GGGACAGTGT CGGCGTTCAG TGCTGGAATC 
CTTAGTGCTG AAGAAGCTGA AGAGCATATG AACAAGCTGC GTTTGATGAC AGAGCAAAAT 
CAACAGACTC CTGGCTTAGC TCTGGCACTT GGAAGTATTG TCCATGGTTT GTCCATTTGT 
GGACATGGGA AGGCAGAAGA TCTAAATAAC AGATTGCTGC CTGCCTGGAT GAAGATTCTT 
CTTGCAGAGG GTTGCCCCAC AATGCAACGG CTGGCGGCTA TTAATGGCCT TGTGGCACTG 
GTAGGATCAG AATCTGCATT GATACAACTT AAATCTGAAG TAATCCATTC CTCACAGTTT 
CAAAGCAAGC TTAACGAAGT AATTAGAGCC ATAACTCAGA TTGTTAGTTT TTCTGGAGCA 
ATCGGACTGC AGACAAATGC CGCATGGATT CTTGGTCATC TACACTTATC CAGCATTTCA 
TCTTCTCAAA GCAGAACGTC TATTCCTCCA GATTTCAGCT ATCTCCCTGA AAGAAGTCTA 
ATTCGGGCTG TAGTGGACTT TCTTATTTCC GCTGGCAAGA AAGGCCCTGA ATCTCTTCCA 
CCTTGCTTAG TGAAGGTCTG TATATCTTCC ATTGCTGCTG CTGCAGAGGG ACATCAATAT 
CCACCCGTTA ACTGGGCATC CATTCTCGCC CCTCTAATGA GACTCAACTT TGGGGAAGAT 
ACCCAGCAAC TTTGTATTCA AATAGCGGTG ACACAGGCCC AAAGCTCACA GAATGCTGCA 
CTGCTTCTTG GGATGTGGCT GGTGCCCCCA CTGGTTTATA GTCTAACAGT ACAGTGTCGT 
TCCTATCTTT TAAACTCCCT ATCTCTGTGG ATGAAGCATG TATCTGAAGA TAAGCTACAG 
ACCTTCTCTG ACTTATTTAT AGTGACTCAG TTTGAGACCC AAAGCAAGAT GGTGGATTTG 
TGTTGGAGCA TTCTACAGGG ACTTAAGCAA GCTATGAAAC TTCCAAACCC CCCGCAGCAT 
TGCTGGAGCT TTCTGTGCAA AACCACAGAG AAAATTGTTC ACCTTTTACC AAGCAAGATT 
CAGAAGACAG ATGTTGGACT GTATGTTGAA GTTGCACAGT GTGTGTCAGA GATGGCAGAT 
AGTGAGGTTG AGCGGATATC TCGCATTTCA AAGGACAACA TAGAGAAAGC TACCTTTTTG 
CGAGCATATC TGGTCTCACA GGGGCGCTTA CCATTGTCAT GCTTGAATGA TGTTATTGAA 
GTGGCAACCA AGTGCAGGGA GAAACAAACC ATCGTATGGA TGCTGCTGCA GGTGTTCTAC 
CAAGCCCGTC TCACAAGTCA CCCAAACACA GGAGTTCTGA AAAGGATGGA ATGGCTGCTT 
GACCTAACTG TTCATATTAG AAATGCAGTC AATCAGAACA TTTCCGTGAG CAGTGTGGAT 
CACTCAGAGG AAGTAGAGTT CCTGCTGCAG GTATTTGCAG CCTCAGTGGT GTCATGGGCT 
GATCATGCAT CTCCCCTGCT TATAGGAGTC AGTGCCAACT GGATGGCATG GAAGGAGGAA 
GAACCATGTA GTACATATTT GGGCACGGGT TCAGTGGATG TGGTAACTGT ACAGCAGTGT 
TTAACCATCC TTCCCCACAG TATGCAACTC TTGGTGGCCA AAGAGCCTTG GAAAGAACAT 
GCCCAAAAGA TCATCGATTG GCTAACAAAC CTTCTTGAGT GTCCCAAGGA AGCCATATTG 
GAAACATCAA GATCCATCTT AAAAGCCACG CTGCTAAGCC TAAGGGGCCT TCCAGAGTTT 
AAGAGGAAAG TTGTGTGGAC AAGGGCCTAC GGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

focad ( XM_012962109.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817563.1 Xenopus tropicalis focadhesin (focad), transcript variant X2, mRNA.

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CloneID	OXa27622
Clone ID Related Accession (Same CDS sequence)	XM_012962109.2 , XM_012962109.3
Accession Version	XM_012962109.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	focadhesin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962109.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAG ATCTCAAGAA GAGATTAGAA TTTCCAAACT CATTAATTCA GACACAGGCG 
GTGACTCAGC TCATTGCATC TGTGTTAAAA GAAAATGTCT CATTGGGGAA GATAAAACAG 
TCTTCAAATC AGACCCCAGC TCTGAATATT CTTTGGGAGA AGTGCTGCAG TAATAATGTG 
GTTGTAAGGA CAGCATGTTG TGAAGCATTG GTGCTACTGG TTGAACAGGA TCATGCTGAT 
TTCGATTATG TTCTTAATGG AATTTTGAAC CTTATACCTT CTGCTGGGAC TGTACAAGGA 
CTGCTGAAAT GTGTATGGCG CCTCCTATAT ACTCAAGCAA GGAATGCAGA AAAGGATGGG 
GAACACAAAG ATTTGGGCAT TTATAAAATT TGGAACCCCC CTCATCCATT CATCAGCATA 
CTGGAAAACA GACCAGACTG TTGGCCTGCA CTTCTGCAGC AGATTTCTAC TTTGCCTCAG 
CTGTGTCATG AAAGCTGTCC TTCAAGCAAT GTTTATTTTA TGGCGTCGTT TTTGAGGTAC 
TTGTATTGTG AACCCTCCCA GCTACAAGAG TACTCTGCCC TTCGAATAGG TCTCAGAAAA 
GCTTTAGTTA TGGTATCGGG GAAGAGTGGG TCCAACAATC CGTCAAAACC AGAAGAGCAC 
ATTCTTCGGA TTTTCATTGA CATGATGGGC TACATTCAGT TTTTGGATTC AACTCAAGTT 
ACTGAAGCAA CTTTGTACAT AAAAGATTTA TGCAGAAGCC TCCACCTGCA TCCAGATTTC 
TGGAGAAATG AGCTCTCTGC AGTTTGCTTG CAGGCCTTAT GTGCATGTGA GGCAAGCTTA 
AAAATTGCAG GAGACTGCTC TACACTAATA GAAATATTGG AACAAAATTC AGATCTGCTT 
GGTGAGAGCT TTCCCACAGA ACAGGTATTA ATTGGACTCA GTTTACTATT GCTACAATCA 
CCAGCTAGCC AGCAAAAACT AATCTTAAAG TTGGTAATGA AGATTCTGTC ATTCAGAGGG 
GAAAACAATT TTCCATCAAT CGTCCAAGTG TTAATTATGC CACTCCTCCA AATTTTATCT 
ACCTCAGCGC TGGGATATTG TTTCTCTGAT GATGGTGGAA GCTCGAGAGA GCAGCTGGCT 
ACTAGTCTTC TGGAAATGGT TCAGAATGCT GACTGTAGTG GGGAAAGGAG ACTGAATGAT 
GTATCACTTC CTCTTCCTGT AACAACATGG CATTGTTCAA TGGCTGCTAC ATTGAACATA 
CTTAACAAGT TTCAAGATGA CCATTCTGCA GCACGTGACT GGCTGTCTTC CATATCTTCG 
ATGCTGGCCC CCAATACTCA AGTTCCTGTC AATGTTTCAC TTTTGCTAAG TTTCCTTCTG 
ATTAGAGAAT CTGATTATGG CTTGCGTAAT GTTTTGAAAG TCATCACTGA TGTCTCCAAA 
GCTGATTCAT CTCAGGTTCC ATATTTAGTT CCAGTTCTAA TGTTGAGACT TGGACATTCC 
TTGGCACCAG AATCCTCCAG AGACATTCTG TACACTTTGC CTACCCTGGG CACCCACAAG 
GTATGTGTGG CTCAGATCTT GCGAGTCTTC CAAATGTTAG GAAGTACTTC AAAGCTTCAA 
CCCATTGCGC TTCGTCTTAT GACATCTTTG TGGGAGAAAC AGGACCGAAT CTATCCAGAA 
TTACAGAAGA TGATGGCCTT ATGTGACACT CAGTCATTAG CCACTGGGAA AGACACATTG 
TGGGAAAAGA TGTTGGCAAA AGCAGCAACT GTCAGAGATA TCTGCAGACA GAGGCCATAT 
CAGCATGGGG CAGATATGCT GGCTGCTATA ACGCAAGTTT TACATGAATG CACAAAAGCC 
GACCAAGCTA CTCCGGCTGC TTTAGCATTG CAGGGTCTTC ATGCTCTCTG TCAAGCAGAG 
GTTGTAGACA TTCGCTCTGC CTGGAATGCT CTTTCCTCCA AACTTAGTTG TGAAACACGT 
CCCCTTGTCC TCAAAGTGTT ACATGAACTT TTTGCATTGG TCCCTTCTTT GACTGTTGAT 
ACAGAAGAAT ATCAGAAATT TAAAACTGAG ATTATCGGTA TCCTTTGGAG GCACACTCAA 
AGTAAGGATC TCAATGTGAT GGTTTCTGCA TACAAATCTT TATGTGAGTT TGAGGCTGAT 
GAGCACTCCA TTCTTCATAT TCCTGAGCAG GCAAGGCCTG AGGTGGCACC TAAAGATGAA 
TCTGACCTGG CTAGTGAAGA AGAGGAGAAA GAAACTGACA TTTCTATCCC TGGCTCATCA 
TATATAAAAT TAATGGCTTT GACACCGGTA TCTGTTTTAC CAGCTCTTGA AGCTTTCCTT 
TCATCTCTTG TAAAGCAAGA AATGAGCCGC ATGCCACGTG GTATTTATCA TACAGCTCTG 
CGTGGTGGTA ATATTAGATC TGACCAAGGC AAAACTATTG CAGGAGTCCC AAACTTCATG 
CTAAAAATGT ACGAGAAGAA TAAACAGCCT GGCCTGAAAC CTGGCCTTGC AGCTGGCATG 
CTGCTGTGTT ACGATCTCCC AATCCAGACA GACAAGGATG GCAGGCCCAT TAATCGTTTC 
CTTGCCAGTA GAGGGCGCAG CTACCAGCAG ATGTTAACCG CACTTATCAA TGAGGTGAAT 
ATTCAGTCTT CAGAATGGCA TCGCTCTATA TTGCTGCCCC AGTCATGGCT TGGCTTCATG 
ACAAGGACAT ACCATGCTGT TCTGCAAGGA AGGCAAGCTG AACTGGAAAT GCAGCTGAAA 
CATGGAAAGG ATGACCCACA AGAGCTTCAG TACAAAACAT TTACAGCTTG GTTGTGGGTA 
AGGGATGCAT TGACCGATGT CATTAGGTGT GCTGTGAAAG ACAGTCCTGT GGTGAAGGGA 
AATTCTCTGT TGGCATTGAC TGGACTTGCT GTGGCTGTAT CAAAATATGA AAGCAGTCTT 
CCATCAGAAA CAGAGGGAGC CCCAGAGATT GAGCCTGATA TTCTTCCCAC AAGTGCTTGG 
ATCACCATGG TTCTTGACAC CCTTTTGAGT ACTGTGGACA ATCATTATCA ACCGAAAGGA 
AGAATCTTCC CTTGGTTCCA ACATAAATCT TACTCTGGAG AAAACACAGC GAGTGTTATT 
GCTAGGTCCT GTGCTGCCAC AGCTCTTTCC CTGCTTGTAC CCGTACTTGT TGTTTCTAAT 
AAAGAGAAGG TGGAAGATAT TCTTAACATC TTGACAGCCA GGCTACCTGG AAAGCCATTA 
GCAGATGACT CTCAGTCGCT TCAGATACAC ATGGGACTTG CGGTTGGCAT GTTCATTTCC 
AGATTGTTCG AGGAGAAAGT CAGTGATGTA TCTGGTCGGC AAATGAATCT ACTAATGATG 
AGGTCTCTGG ATGCACTGGA AAGTTGCTGC TTTGACCAAA GCCTGGAATA CAACATGGGC 
TGCATTCTGG GAGTGGGCCT TGTTTTGTCT TTTATGTGTC AGAGCAGCCA AACTGATAGC 
CAGGTTCATG TTTCTACTAC TCTGCGCAAA TTTTATATGG CCTTGAGTGA AGACAAGAGC 
AGAACAGAGC AAGAAGGTTT GTCGTACTCT TTGGCCTGTG GGACAGTGTC GGCGTTCAGT 
GCTGGAATCC TTAGTGCTGA AGAAGCTGAA GAGCATATGA ACAAGCTGCG TTTGATGACA 
GAGCAAAATC AACAGACTCC TGGCTTAGCT CTGGCACTTG GAAGTATTGT CCATGGTTTG 
TCCATTTGTG GACATGGGAA GGCAGAAGAT CTAAATAACA GATTGCTGCC TGCCTGGATG 
AAGATTCTTC TTGCAGAGGG TTGCCCCACA ATGCAACGGC TGGCGGCTAT TAATGGCCTT 
GTGGCACTGG TAGGATCAGA ATCTGCATTG ATACAACTTA AATCTGAAGT AATCCATTCC 
TCACAGTTTC AAAGCAAGCT TAACGAAGTA ATTAGAGCCA TAACTCAGAT TGTTAGTTTT 
TCTGGAGCAA TCGGACTGCA GACAAATGCC GCATGGATTC TTGGTCATCT ACACTTATCC 
AGCATTTCAT CTTCTCAAAG CAGAACGTCT ATTCCTCCAG ATTTCAGCTA TCTCCCTGAA 
AGAAGTCTAA TTCGGGCTGT AGTGGACTTT CTTATTTCCG CTGGCAAGAA AGGCCCTGAA 
TCTCTTCCAC CTTGCTTAGT GAAGGTCTGT ATATCTTCCA TTGCTGCTGC TGCAGAGGGA 
CATCAATATC CACCCGTTAA CTGGGCATCC ATTCTCGCCC CTCTAATGAG ACTCAACTTT 
GGGGAAGATA CCCAGCAACT TTGTATTCAA ATAGCGGTGA CACAGGCCCA AAGCTCACAG 
AATGCTGCAC TGCTTCTTGG GATGTGGCTG GTGCCCCCAC TGGTTTATAG TCTAACAGTA 
CAGTGTCGTT CCTATCTTTT AAACTCCCTA TCTCTGTGGA TGAAGCATGT ATCTGAAGAT 
AAGCTACAGA CCTTCTCTGA CTTATTTATA GTGACTCAGT TTGAGACCCA AAGCAAGATG 
GTGGATTTGT GTTGGAGCAT TCTACAGGGA CTTAAGCAAG CTATGAAACT TCCAAACCCC 
CCGCAGCATT GCTGGAGCTT TCTGTGCAAA ACCACAGAGA AAATTGTTCA CCTTTTACCA 
AGCAAGATTC AGAAGACAGA TGTTGGACTG TATGTTGAAG TTGCACAGTG TGTGTCAGAG 
ATGGCAGATA GTGAGGTTGA GCGGATATCT CGCATTTCAA AGGACAACAT AGAGAAAGCT 
ACCTTTTTGC GAGCATATCT GGTCTCACAG GGGCGCTTAC CATTGTCATG CTTGAATGAT 
GTTATTGAAG TGGCAACCAA GTGCAGGGAG AAACAAACCA TCGTATGGAT GCTGCTGCAG 
GTGTTCTACC AAGCCCGTCT CACAAGTCAC CCAAACACAG GAGTTCTGAA AAGGATGGAA 
TGGCTGCTTG ACCTAACTGT TCATATTAGA AATGCAGTCA ATCAGAACAT TTCCGTGAGC 
AGTGTGGATC ACTCAGAGGA AGTAGAGTTC CTGCTGCAGG TATTTGCAGC CTCAGTGGTG 
TCATGGGCTG ATCATGCATC TCCCCTGCTT ATAGGAGTCA GTGCCAACTG GATGGCATGG 
AAGGAGGAAG AACCATGTAG TACATATTTG GGCACGGGTT CAGTGGATGT GGTAACTGTA 
CAGCAGTGTT TAACCATCCT TCCCCACAGT ATGCAACTCT TGGTGGCCAA AGAGCCTTGG 
AAAGAACATG CCCAAAAGAT CATCGATTGG CTAACAAACC TTCTTGAGTG TCCCAAGGAA 
GCCATATTGG AAACATCAAG ATCCATCTTA AAAGCCACGC TGCTAAGCCT AAGGGGCCTT 
CCAGAGTTTA AGAGGAAAGT TGTGTGGACA AGGGCCTACG GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817563.1
CDS	260..5647
Translation	MSEDLKKRLEFPNSLIQTQAVTQLIASVLKENVSLGKIKQSSNQTPALNILWEKCCSNNVVVRTACCEALVLLVEQDHADFDYVLNGILNLIPSAGTVQGLLKCVWRLLYTQARNAEKDGEHKDLGIYKIWNPPHPFISILENRPDCWPALLQQISTLPQLCHESCPSSNVYFMASFLRYLYCEPSQLQEYSALRIGLRKALVMVSGKSGSNNPSKPEEHILRIFIDMMGYIQFLDSTQVTEATLYIKDLCRSLHLHPDFWRNELSAVCLQALCACEASLKIAGDCSTLIEILEQNSDLLGESFPTEQVLIGLSLLLLQSPASQQKLILKLVMKILSFRGENNFPSIVQVLIMPLLQILSTSALGYCFSDDGGSSREQLATSLLEMVQNADCSGERRLNDVSLPLPVTTWHCSMAATLNILNKFQDDHSAARDWLSSISSMLAPNTQVPVNVSLLLSFLLIRESDYGLRNVLKVITDVSKADSSQVPYLVPVLMLRLGHSLAPESSRDILYTLPTLGTHKVCVAQILRVFQMLGSTSKLQPIALRLMTSLWEKQDRIYPELQKMMALCDTQSLATGKDTLWEKMLAKAATVRDICRQRPYQHGADMLAAITQVLHECTKADQATPAALALQGLHALCQAEVVDIRSAWNALSSKLSCETRPLVLKVLHELFALVPSLTVDTEEYQKFKTEIIGILWRHTQSKDLNVMVSAYKSLCEFEADEHSILHIPEQARPEVAPKDESDLASEEEEKETDISIPGSSYIKLMALTPVSVLPALEAFLSSLVKQEMSRMPRGIYHTALRGGNIRSDQGKTIAGVPNFMLKMYEKNKQPGLKPGLAAGMLLCYDLPIQTDKDGRPINRFLASRGRSYQQMLTALINEVNIQSSEWHRSILLPQSWLGFMTRTYHAVLQGRQAELEMQLKHGKDDPQELQYKTFTAWLWVRDALTDVIRCAVKDSPVVKGNSLLALTGLAVAVSKYESSLPSETEGAPEIEPDILPTSAWITMVLDTLLSTVDNHYQPKGRIFPWFQHKSYSGENTASVIARSCAATALSLLVPVLVVSNKEKVEDILNILTARLPGKPLADDSQSLQIHMGLAVGMFISRLFEEKVSDVSGRQMNLLMMRSLDALESCCFDQSLEYNMGCILGVGLVLSFMCQSSQTDSQVHVSTTLRKFYMALSEDKSRTEQEGLSYSLACGTVSAFSAGILSAEEAEEHMNKLRLMTEQNQQTPGLALALGSIVHGLSICGHGKAEDLNNRLLPAWMKILLAEGCPTMQRLAAINGLVALVGSESALIQLKSEVIHSSQFQSKLNEVIRAITQIVSFSGAIGLQTNAAWILGHLHLSSISSSQSRTSIPPDFSYLPERSLIRAVVDFLISAGKKGPESLPPCLVKVCISSIAAAAEGHQYPPVNWASILAPLMRLNFGEDTQQLCIQIAVTQAQSSQNAALLLGMWLVPPLVYSLTVQCRSYLLNSLSLWMKHVSEDKLQTFSDLFIVTQFETQSKMVDLCWSILQGLKQAMKLPNPPQHCWSFLCKTTEKIVHLLPSKIQKTDVGLYVEVAQCVSEMADSEVERISRISKDNIEKATFLRAYLVSQGRLPLSCLNDVIEVATKCREKQTIVWMLLQVFYQARLTSHPNTGVLKRMEWLLDLTVHIRNAVNQNISVSSVDHSEEVEFLLQVFAASVVSWADHASPLLIGVSANWMAWKEEEPCSTYLGTGSVDVVTVQQCLTILPHSMQLLVAKEPWKEHAQKIIDWLTNLLECPKEAILETSRSILKATLLSLRGLPEFKRKVVWTRAYGW

Target ORF information:

RefSeq Version	XM_012962109.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis focadhesin (focad), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962109.2

ORF Insert Sequence:

ATGTCAGAAG ATCTCAAGAA GAGATTAGAA TTTCCAAACT CATTAATTCA GACACAGGCG 
GTGACTCAGC TCATTGCATC TGTGTTAAAA GAAAATGTCT CATTGGGGAA GATAAAACAG 
TCTTCAAATC AGACCCCAGC TCTGAATATT CTTTGGGAGA AGTGCTGCAG TAATAATGTG 
GTTGTAAGGA CAGCATGTTG TGAAGCATTG GTGCTACTGG TTGAACAGGA TCATGCTGAT 
TTCGATTATG TTCTTAATGG AATTTTGAAC CTTATACCTT CTGCTGGGAC TGTACAAGGA 
CTGCTGAAAT GTGTATGGCG CCTCCTATAT ACTCAAGCAA GGAATGCAGA AAAGGATGGG 
GAACACAAAG ATTTGGGCAT TTATAAAATT TGGAACCCCC CTCATCCATT CATCAGCATA 
CTGGAAAACA GACCAGACTG TTGGCCTGCA CTTCTGCAGC AGATTTCTAC TTTGCCTCAG 
CTGTGTCATG AAAGCTGTCC TTCAAGCAAT GTTTATTTTA TGGCGTCGTT TTTGAGGTAC 
TTGTATTGTG AACCCTCCCA GCTACAAGAG TACTCTGCCC TTCGAATAGG TCTCAGAAAA 
GCTTTAGTTA TGGTATCGGG GAAGAGTGGG TCCAACAATC CGTCAAAACC AGAAGAGCAC 
ATTCTTCGGA TTTTCATTGA CATGATGGGC TACATTCAGT TTTTGGATTC AACTCAAGTT 
ACTGAAGCAA CTTTGTACAT AAAAGATTTA TGCAGAAGCC TCCACCTGCA TCCAGATTTC 
TGGAGAAATG AGCTCTCTGC AGTTTGCTTG CAGGCCTTAT GTGCATGTGA GGCAAGCTTA 
AAAATTGCAG GAGACTGCTC TACACTAATA GAAATATTGG AACAAAATTC AGATCTGCTT 
GGTGAGAGCT TTCCCACAGA ACAGGTATTA ATTGGACTCA GTTTACTATT GCTACAATCA 
CCAGCTAGCC AGCAAAAACT AATCTTAAAG TTGGTAATGA AGATTCTGTC ATTCAGAGGG 
GAAAACAATT TTCCATCAAT CGTCCAAGTG TTAATTATGC CACTCCTCCA AATTTTATCT 
ACCTCAGCGC TGGGATATTG TTTCTCTGAT GATGGTGGAA GCTCGAGAGA GCAGCTGGCT 
ACTAGTCTTC TGGAAATGGT TCAGAATGCT GACTGTAGTG GGGAAAGGAG ACTGAATGAT 
GTATCACTTC CTCTTCCTGT AACAACATGG CATTGTTCAA TGGCTGCTAC ATTGAACATA 
CTTAACAAGT TTCAAGATGA CCATTCTGCA GCACGTGACT GGCTGTCTTC CATATCTTCG 
ATGCTGGCCC CCAATACTCA AGTTCCTGTC AATGTTTCAC TTTTGCTAAG TTTCCTTCTG 
ATTAGAGAAT CTGATTATGG CTTGCGTAAT GTTTTGAAAG TCATCACTGA TGTCTCCAAA 
GCTGATTCAT CTCAGGTTCC ATATTTAGTT CCAGTTCTAA TGTTGAGACT TGGACATTCC 
TTGGCACCAG AATCCTCCAG AGACATTCTG TACACTTTGC CTACCCTGGG CACCCACAAG 
GTATGTGTGG CTCAGATCTT GCGAGTCTTC CAAATGTTAG GAAGTACTTC AAAGCTTCAA 
CCCATTGCGC TTCGTCTTAT GACATCTTTG TGGGAGAAAC AGGACCGAAT CTATCCAGAA 
TTACAGAAGA TGATGGCCTT ATGTGACACT CAGTCATTAG CCACTGGGAA AGACACATTG 
TGGGAAAAGA TGTTGGCAAA AGCAGCAACT GTCAGAGATA TCTGCAGACA GAGGCCATAT 
CAGCATGGGG CAGATATGCT GGCTGCTATA ACGCAAGTTT TACATGAATG CACAAAAGCC 
GACCAAGCTA CTCCGGCTGC TTTAGCATTG CAGGGTCTTC ATGCTCTCTG TCAAGCAGAG 
GTTGTAGACA TTCGCTCTGC CTGGAATGCT CTTTCCTCCA AACTTAGTTG TGAAACACGT 
CCCCTTGTCC TCAAAGTGTT ACATGAACTT TTTGCATTGG TCCCTTCTTT GACTGTTGAT 
ACAGAAGAAT ATCAGAAATT TAAAACTGAG ATTATCGGTA TCCTTTGGAG GCACACTCAA 
AGTAAGGATC TCAATGTGAT GGTTTCTGCA TACAAATCTT TATGTGAGTT TGAGGCTGAT 
GAGCACTCCA TTCTTCATAT TCCTGAGCAG GCAAGGCCTG AGGTGGCACC TAAAGATGAA 
TCTGACCTGG CTAGTGAAGA AGAGGAGAAA GAAACTGACA TTTCTATCCC TGGCTCATCA 
TATATAAAAT TAATGGCTTT GACACCGGTA TCTGTTTTAC CAGCTCTTGA AGCTTTCCTT 
TCATCTCTTG TAAAGCAAGA AATGAGCCGC ATGCCACGTG GTATTTATCA TACAGCTCTG 
CGTGGTGGTA ATATTAGATC TGACCAAGGC AAAACTATTG CAGGAGTCCC AAACTTCATG 
CTAAAAATGT ACGAGAAGAA TAAACAGCCT GGCCTGAAAC CTGGCCTTGC AGCTGGCATG 
CTGCTGTGTT ACGATCTCCC AATCCAGACA GACAAGGATG GCAGGCCCAT TAATCGTTTC 
CTTGCCAGTA GAGGGCGCAG CTACCAGCAG ATGTTAACCG CACTTATCAA TGAGGTGAAT 
ATTCAGTCTT CAGAATGGCA TCGCTCTATA TTGCTGCCCC AGTCATGGCT TGGCTTCATG 
ACAAGGACAT ACCATGCTGT TCTGCAAGGA AGGCAAGCTG AACTGGAAAT GCAGCTGAAA 
CATGGAAAGG ATGACCCACA AGAGCTTCAG TACAAAACAT TTACAGCTTG GTTGTGGGTA 
AGGGATGCAT TGACCGATGT CATTAGGTGT GCTGTGAAAG ACAGTCCTGT GGTGAAGGGA 
AATTCTCTGT TGGCATTGAC TGGACTTGCT GTGGCTGTAT CAAAATATGA AAGCAGTCTT 
CCATCAGAAA CAGAGGGAGC CCCAGAGATT GAGCCTGATA TTCTTCCCAC AAGTGCTTGG 
ATCACCATGG TTCTTGACAC CCTTTTGAGT ACTGTGGACA ATCATTATCA ACCGAAAGGA 
AGAATCTTCC CTTGGTTCCA ACATAAATCT TACTCTGGAG AAAACACAGC GAGTGTTATT 
GCTAGGTCCT GTGCTGCCAC AGCTCTTTCC CTGCTTGTAC CCGTACTTGT TGTTTCTAAT 
AAAGAGAAGG TGGAAGATAT TCTTAACATC TTGACAGCCA GGCTACCTGG AAAGCCATTA 
GCAGATGACT CTCAGTCGCT TCAGATACAC ATGGGACTTG CGGTTGGCAT GTTCATTTCC 
AGATTGTTCG AGGAGAAAGT CAGTGATGTA TCTGGTCGGC AAATGAATCT ACTAATGATG 
AGGTCTCTGG ATGCACTGGA AAGTTGCTGC TTTGACCAAA GCCTGGAATA CAACATGGGC 
TGCATTCTGG GAGTGGGCCT TGTTTTGTCT TTTATGTGTC AGAGCAGCCA AACTGATAGC 
CAGGTTCATG TTTCTACTAC TCTGCGCAAA TTTTATATGG CCTTGAGTGA AGACAAGAGC 
AGAACAGAGC AAGAAGGTTT GTCGTACTCT TTGGCCTGTG GGACAGTGTC GGCGTTCAGT 
GCTGGAATCC TTAGTGCTGA AGAAGCTGAA GAGCATATGA ACAAGCTGCG TTTGATGACA 
GAGCAAAATC AACAGACTCC TGGCTTAGCT CTGGCACTTG GAAGTATTGT CCATGGTTTG 
TCCATTTGTG GACATGGGAA GGCAGAAGAT CTAAATAACA GATTGCTGCC TGCCTGGATG 
AAGATTCTTC TTGCAGAGGG TTGCCCCACA ATGCAACGGC TGGCGGCTAT TAATGGCCTT 
GTGGCACTGG TAGGATCAGA ATCTGCATTG ATACAACTTA AATCTGAAGT AATCCATTCC 
TCACAGTTTC AAAGCAAGCT TAACGAAGTA ATTAGAGCCA TAACTCAGAT TGTTAGTTTT 
TCTGGAGCAA TCGGACTGCA GACAAATGCC GCATGGATTC TTGGTCATCT ACACTTATCC 
AGCATTTCAT CTTCTCAAAG CAGAACGTCT ATTCCTCCAG ATTTCAGCTA TCTCCCTGAA 
AGAAGTCTAA TTCGGGCTGT AGTGGACTTT CTTATTTCCG CTGGCAAGAA AGGCCCTGAA 
TCTCTTCCAC CTTGCTTAGT GAAGGTCTGT ATATCTTCCA TTGCTGCTGC TGCAGAGGGA 
CATCAATATC CACCCGTTAA CTGGGCATCC ATTCTCGCCC CTCTAATGAG ACTCAACTTT 
GGGGAAGATA CCCAGCAACT TTGTATTCAA ATAGCGGTGA CACAGGCCCA AAGCTCACAG 
AATGCTGCAC TGCTTCTTGG GATGTGGCTG GTGCCCCCAC TGGTTTATAG TCTAACAGTA 
CAGTGTCGTT CCTATCTTTT AAACTCCCTA TCTCTGTGGA TGAAGCATGT ATCTGAAGAT 
AAGCTACAGA CCTTCTCTGA CTTATTTATA GTGACTCAGT TTGAGACCCA AAGCAAGATG 
GTGGATTTGT GTTGGAGCAT TCTACAGGGA CTTAAGCAAG CTATGAAACT TCCAAACCCC 
CCGCAGCATT GCTGGAGCTT TCTGTGCAAA ACCACAGAGA AAATTGTTCA CCTTTTACCA 
AGCAAGATTC AGAAGACAGA TGTTGGACTG TATGTTGAAG TTGCACAGTG TGTGTCAGAG 
ATGGCAGATA GTGAGGTTGA GCGGATATCT CGCATTTCAA AGGACAACAT AGAGAAAGCT 
ACCTTTTTGC GAGCATATCT GGTCTCACAG GGGCGCTTAC CATTGTCATG CTTGAATGAT 
GTTATTGAAG TGGCAACCAA GTGCAGGGAG AAACAAACCA TCGTATGGAT GCTGCTGCAG 
GTGTTCTACC AAGCCCGTCT CACAAGTCAC CCAAACACAG GAGTTCTGAA AAGGATGGAA 
TGGCTGCTTG ACCTAACTGT TCATATTAGA AATGCAGTCA ATCAGAACAT TTCCGTGAGC 
AGTGTGGATC ACTCAGAGGA AGTAGAGTTC CTGCTGCAGG TATTTGCAGC CTCAGTGGTG 
TCATGGGCTG ATCATGCATC TCCCCTGCTT ATAGGAGTCA GTGCCAACTG GATGGCATGG 
AAGGAGGAAG AACCATGTAG TACATATTTG GGCACGGGTT CAGTGGATGT GGTAACTGTA 
CAGCAGTGTT TAACCATCCT TCCCCACAGT ATGCAACTCT TGGTGGCCAA AGAGCCTTGG 
AAAGAACATG CCCAAAAGAT CATCGATTGG CTAACAAACC TTCTTGAGTG TCCCAAGGAA 
GCCATATTGG AAACATCAAG ATCCATCTTA AAAGCCACGC TGCTAAGCCT AAGGGGCCTT 
CCAGAGTTTA AGAGGAAAGT TGTGTGGACA AGGGCCTACG GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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focad cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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