tle2 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012961727.2	XP_012817181.1	transducin like enhancer of split 2 isoform X3
XM_012961733.3	XP_012817187.1	transducin like enhancer of split 2 isoform X4
XM_012961741.2	XP_012817195.1	transducin like enhancer of split 2 isoform X5
XM_012961752.2	XP_012817206.1	transducin like enhancer of split 2 isoform X6
XM_012961720.3	XP_012817174.1	transducin like enhancer of split 2 isoform X2
XM_012961741.3	XP_012817195.1	transducin like enhancer of split 2 isoform X5
XM_012961745.2	XP_012817199.1	transducin like enhancer of split 2 isoform X5
XM_012961716.2	XP_012817170.1	transducin like enhancer of split 2 isoform X1
XM_012961733.2	XP_012817187.1	transducin like enhancer of split 2 isoform X4
XM_012961736.2	XP_012817190.1	transducin like enhancer of split 2 isoform X5
XM_012961756.2	XP_012817210.1	transducin like enhancer of split 2 isoform X6
XM_012961716.3	XP_012817170.1	transducin like enhancer of split 2 isoform X1
XM_012961727.3	XP_012817181.1	transducin like enhancer of split 2 isoform X3
XM_012961720.2	XP_012817174.1	transducin like enhancer of split 2 isoform X2
XM_012961736.3	XP_012817190.1	transducin like enhancer of split 2 isoform X5
XM_012961752.3	XP_012817206.1	transducin like enhancer of split 2 isoform X6
NM_001015842.1	NP_001015842.1	transducin like enhancer of split 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa02096	NM_001015842.1 Latest version!	Xenopus tropicalis transducin like enhancer of split 2 (tle2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa27440	XM_012961745.2 Latest version!	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa27441	XM_012961756.2 Latest version!	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa27436	XM_012961716.3 Latest version!	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa27437	XM_012961720.3 Latest version!	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa27438	XM_012961727.3 Latest version!	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa27439	XM_012961733.3 Latest version!	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa27440	XM_012961741.3 Latest version!	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa27440	XM_012961736.3 Latest version!	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa27441	XM_012961752.3 Latest version!	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa27440	XM_012961741.2	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa27439	XM_012961733.2	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa27436	XM_012961716.2	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa27438	XM_012961727.2	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa27437	XM_012961720.2	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa27440	XM_012961736.2	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa27441	XM_012961752.2	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

tle2 ( NM_001015842.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001015842.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), mRNA.

Price & Availability↑

CloneID	OXa02096
Clone ID Related Accession (Same CDS sequence)	NM_001015842.1
Accession Version	NM_001015842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC090131.1. ##Evidence-Data-START## Transcript exon combination :: BC090131.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGG TACGTTTCAT TCCCCCTCAA 
CTGACTGTGC ACCAGCTGCA GGCCCAGCAC CTCTCTCACC ATGCACCTCC AATCCCTTTA 
ACCCCACATC CATCCAGCCT GCAGCCCCCG GGACTTGCTG CCGCAACCAG TGCCTCAAGC 
CTATTGGCAC TGTCTGGTGT TCTTGGCCTT CAGACACAAC TGGCTGTGAA AGAGGGCCTT 
GCGGATGCAG AACACAGAGA GAGAGACCCA GGGCCTAGCT GTGTAACTCT TCCTAATGGA 
GAGCGGGTGC GCACTCTGTC AGATTACCTA AACAGCAACA AGAAGCGGAA AACAGAAGAA 
AAGGATTTTG GCACAGACTA TGGTAGTGAT GCTGACCGCA GCGAAGATAA TTTGGTGGTT 
GATGAGGATC CTGCTTCTCC TCATAGTGTC CATTCTTACT CCTCTCGGGA AAATGGTGTG 
GACAAACCTA GTCTACAGAG AAAAGACCCT CCACCTGCAA GCCCAAACTC CATGACCTCT 
TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA GACATTCCCT CTCAAGTGGA GAAGGCAGGG 
ACCCCTGGCT TAAAGTCTAG CACTCCTAAT TCACAGAGCG ATATGAATAC ACCGGGCCCA 
AGTGGAACCA GTGCTTCACA GTTTAGGTCT ATCTCCTCCA AACCAGCCAT TGACTCATTA 
GCTCTTGGAC TGCGGACTCC ATTAGGGGGG CAGGGAGGTT ATCCTGCTGC ATTTAGCATT 
GCTCACCCGT CTGTGAATGC AGATGGTGCT GGTGCCTATG CTGGGTTGCA CCTCATGTCT 
CCACAAATTA ATGGTGCCAC TGCAGCTGGA ACCTATGGAC GATCTCCTTT AGTCAGCTAT 
GACCCCCATG CTCACATGAG AGGATTGGGT GCTGCAATTC CAGGGTCTGC ATCAGGAAAA 
CCGGCATACT CTTTTCATGT CAGTGCAGAT GGACAAATGC AGCCAGTTCC CTTTCCCCCA 
GATGCTCTTA TCGGCTCAGG AATTCCACGG CACGCACGTC AGCTCCACGT ACTGAACCAT 
GGGGAGGTAG TCTGTGCGGT GACAATAAGC AACTCAACAC GCCATGTCTA TACTGGAGGC 
AAAGGCTGTG TTAAAGTGTG GGATGTTGGC CAACCGGGTA CAAAGACTCC TGTGGCACAG 
CTTGATTGCC TGAACCGTGA CAATTACATT CGCTCCTGTA AACTTCTGCC TGATGGACGC 
AGCCTCATTG TTGGCGGGGA AGCCAGCACC TTGTCCATCT GGGACTTGGC ATCTCCTACC 
CCTAGAATAA AGGCAGAGCT TACATCCTCA GCCCCAGCCT GCTACGCTCT TGCTATTAGC 
CCTGATGCCA AAGTTTGCTT CTCTTGCTGC TCTGATGGAA ACATTGTAGT GTGGGACCTT 
CAAAACCAGA CCCTTGTCAG GCAGTTTCAG GGCCATACGG ATGGCGCCAG CTGCATAGAT 
ATCTCTCACG ATGGTACAAA ATTATGGACT GGGGGATTGG ATAACACAGT GCGATGCTGG 
GACCTGAGGG AGGGCAGACA GCTCCAACAA CATGACTTCA ATTCACAGAT CTTTTCTCTG 
GGGTATTGCC CCACTGGGGA ATGGCTGGCT GTGGGCATGG AGAGCAGCAA TGTTGAGGTT 
CTGCACGTTT CCAAACCTGA TAAGTACCAA CTGCATCTTC ACGAGAGCTG TGTCCTGTCC 
CTGCAATTTG CTTCTTGTGG GAAATGGTTT GTGAGTACTG GGAAGGACAA TTTGCTAAAT 
GCTTGGAGGA CGCCATATGG GGCCAGCATA TTCCAGTCAA AAGAATCCTC TTCTGTTCTG 
AGCTGTGACG TTTCCACTGA TGACCAATTC ATTGTCACAG GTTCTGGAGA CAAAAAGGCG 
ACAGTATATG AGGTTATCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015842.1
CDS	135..2438
Translation	MFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGVRFIPPQLTVHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	NM_001015842.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015842.1

ORF Insert Sequence:

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGG TACGTTTCAT TCCCCCTCAA 
CTGACTGTGC ACCAGCTGCA GGCCCAGCAC CTCTCTCACC ATGCACCTCC AATCCCTTTA 
ACCCCACATC CATCCAGCCT GCAGCCCCCG GGACTTGCTG CCGCAACCAG TGCCTCAAGC 
CTATTGGCAC TGTCTGGTGT TCTTGGCCTT CAGACACAAC TGGCTGTGAA AGAGGGCCTT 
GCGGATGCAG AACACAGAGA GAGAGACCCA GGGCCTAGCT GTGTAACTCT TCCTAATGGA 
GAGCGGGTGC GCACTCTGTC AGATTACCTA AACAGCAACA AGAAGCGGAA AACAGAAGAA 
AAGGATTTTG GCACAGACTA TGGTAGTGAT GCTGACCGCA GCGAAGATAA TTTGGTGGTT 
GATGAGGATC CTGCTTCTCC TCATAGTGTC CATTCTTACT CCTCTCGGGA AAATGGTGTG 
GACAAACCTA GTCTACAGAG AAAAGACCCT CCACCTGCAA GCCCAAACTC CATGACCTCT 
TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA GACATTCCCT CTCAAGTGGA GAAGGCAGGG 
ACCCCTGGCT TAAAGTCTAG CACTCCTAAT TCACAGAGCG ATATGAATAC ACCGGGCCCA 
AGTGGAACCA GTGCTTCACA GTTTAGGTCT ATCTCCTCCA AACCAGCCAT TGACTCATTA 
GCTCTTGGAC TGCGGACTCC ATTAGGGGGG CAGGGAGGTT ATCCTGCTGC ATTTAGCATT 
GCTCACCCGT CTGTGAATGC AGATGGTGCT GGTGCCTATG CTGGGTTGCA CCTCATGTCT 
CCACAAATTA ATGGTGCCAC TGCAGCTGGA ACCTATGGAC GATCTCCTTT AGTCAGCTAT 
GACCCCCATG CTCACATGAG AGGATTGGGT GCTGCAATTC CAGGGTCTGC ATCAGGAAAA 
CCGGCATACT CTTTTCATGT CAGTGCAGAT GGACAAATGC AGCCAGTTCC CTTTCCCCCA 
GATGCTCTTA TCGGCTCAGG AATTCCACGG CACGCACGTC AGCTCCACGT ACTGAACCAT 
GGGGAGGTAG TCTGTGCGGT GACAATAAGC AACTCAACAC GCCATGTCTA TACTGGAGGC 
AAAGGCTGTG TTAAAGTGTG GGATGTTGGC CAACCGGGTA CAAAGACTCC TGTGGCACAG 
CTTGATTGCC TGAACCGTGA CAATTACATT CGCTCCTGTA AACTTCTGCC TGATGGACGC 
AGCCTCATTG TTGGCGGGGA AGCCAGCACC TTGTCCATCT GGGACTTGGC ATCTCCTACC 
CCTAGAATAA AGGCAGAGCT TACATCCTCA GCCCCAGCCT GCTACGCTCT TGCTATTAGC 
CCTGATGCCA AAGTTTGCTT CTCTTGCTGC TCTGATGGAA ACATTGTAGT GTGGGACCTT 
CAAAACCAGA CCCTTGTCAG GCAGTTTCAG GGCCATACGG ATGGCGCCAG CTGCATAGAT 
ATCTCTCACG ATGGTACAAA ATTATGGACT GGGGGATTGG ATAACACAGT GCGATGCTGG 
GACCTGAGGG AGGGCAGACA GCTCCAACAA CATGACTTCA ATTCACAGAT CTTTTCTCTG 
GGGTATTGCC CCACTGGGGA ATGGCTGGCT GTGGGCATGG AGAGCAGCAA TGTTGAGGTT 
CTGCACGTTT CCAAACCTGA TAAGTACCAA CTGCATCTTC ACGAGAGCTG TGTCCTGTCC 
CTGCAATTTG CTTCTTGTGG GAAATGGTTT GTGAGTACTG GGAAGGACAA TTTGCTAAAT 
GCTTGGAGGA CGCCATATGG GGCCAGCATA TTCCAGTCAA AAGAATCCTC TTCTGTTCTG 
AGCTGTGACG TTTCCACTGA TGACCAATTC ATTGTCACAG GTTCTGGAGA CAAAAAGGCG 
ACAGTATATG AGGTTATCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961716.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817170.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa27436
Clone ID Related Accession (Same CDS sequence)	XM_012961716.2 , XM_012961716.3
Accession Version	XM_012961716.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGGTACGTTT CATTCCCCCT 
CAACTGACTG TGCACCAGCT GCAGGCCCAG CACCTCTCTC ACCATGCACC TCCAATCCCT 
TTAACCCCAC ATCCATCCAG CCTGCAGCCC CCGGGACTTG CTGCCGCAAC CAGTGCCTCA 
AGCCTATTGG CACTGTCTGG TGTTCTTGGC CTTCAGACAC AACTGGCTGT GAAAGAGGGC 
CTTGCGGATG CAGAACACAG AGAGAGAGAC CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT 
GGAGAGCGGG TGCGCACTCT GTCAGATTAC CTAAACAGCA ACAAGAAGCG GAAAACAGAA 
GAAAAGGATT TTGGCACAGA CTATGGTAGT GATGCTGACC GCAGCGAAGA TAATTTGGTG 
GTTGATGAGG ATCCTGCTTC TCCTCATAGT GTCCATTCTT ACTCCTCTCG GGAAAATGGT 
GTGGACAAAC CTAGTCTACA GAGAAAAGAC CCTCCACCTG CAAGCCCAAA CTCCATGACC 
TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC AAAGACATTC CCTCTCAGCA AGTGGAGAAG 
GCAGGGACCC CTGGCTTAAA GTCTAGCACT CCTAATTCAC AGAGCGATAT GAATACACCG 
GGCCCAAGTG GAACCAGTGC TTCACAGTTT AGGTCTATCT CCTCCAAACC AGCCATTGAC 
TCATTAGCTC TTGGACTGCG GACTCCATTA GGGGGGCAGG GAGGTTATCC TGCTGCATTT 
AGCATTGCTC ACCCGTCTGT GAATGCAGAT GGTGCTGGTG CCTATGCTGG GTTGCACCTC 
ATGTCTCCAC AAATTAATGG TGCCACTGCA GCTGGAACCT ATGGACGATC TCCTTTAGTC 
AGCTATGACC CCCATGCTCA CATGAGAGGA TTGGGTGCTG CAATTCCAGG GTCTGCATCA 
GGAAAACCGG CATACTCTTT TCATGTCAGT GCAGATGGAC AAATGCAGCC AGTTCCCTTT 
CCCCCAGATG CTCTTATCGG CTCAGGAATT CCACGGCACG CACGTCAGCT CCACGTACTG 
AACCATGGGG AGGTAGTCTG TGCGGTGACA ATAAGCAACT CAACACGCCA TGTCTATACT 
GGAGGCAAAG GCTGTGTTAA AGTGTGGGAT GTTGGCCAAC CGGGTACAAA GACTCCTGTG 
GCACAGCTTG ATTGCCTGAA CCGTGACAAT TACATTCGCT CCTGTAAACT TCTGCCTGAT 
GGACGCAGCC TCATTGTTGG CGGGGAAGCC AGCACCTTGT CCATCTGGGA CTTGGCATCT 
CCTACCCCTA GAATAAAGGC AGAGCTTACA TCCTCAGCCC CAGCCTGCTA CGCTCTTGCT 
ATTAGCCCTG ATGCCAAAGT TTGCTTCTCT TGCTGCTCTG ATGGAAACAT TGTAGTGTGG 
GACCTTCAAA ACCAGACCCT TGTCAGGCAG TTTCAGGGCC ATACGGATGG CGCCAGCTGC 
ATAGATATCT CTCACGATGG TACAAAATTA TGGACTGGGG GATTGGATAA CACAGTGCGA 
TGCTGGGACC TGAGGGAGGG CAGACAGCTC CAACAACATG ACTTCAATTC ACAGATCTTT 
TCTCTGGGGT ATTGCCCCAC TGGGGAATGG CTGGCTGTGG GCATGGAGAG CAGCAATGTT 
GAGGTTCTGC ACGTTTCCAA ACCTGATAAG TACCAACTGC ATCTTCACGA GAGCTGTGTC 
CTGTCCCTGC AATTTGCTTC TTGTGGGAAA TGGTTTGTGA GTACTGGGAA GGACAATTTG 
CTAAATGCTT GGAGGACGCC ATATGGGGCC AGCATATTCC AGTCAAAAGA ATCCTCTTCT 
GTTCTGAGCT GTGACGTTTC CACTGATGAC CAATTCATTG TCACAGGTTC TGGAGACAAA 
AAGGCGACAG TATATGAGGT TATCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817170.1
CDS	44..2413
Translation	MREQGKGPIAQTETVPLPPSKMFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGVRFIPPQLTVHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961716.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961716.2

ORF Insert Sequence:

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGGTACGTTT CATTCCCCCT 
CAACTGACTG TGCACCAGCT GCAGGCCCAG CACCTCTCTC ACCATGCACC TCCAATCCCT 
TTAACCCCAC ATCCATCCAG CCTGCAGCCC CCGGGACTTG CTGCCGCAAC CAGTGCCTCA 
AGCCTATTGG CACTGTCTGG TGTTCTTGGC CTTCAGACAC AACTGGCTGT GAAAGAGGGC 
CTTGCGGATG CAGAACACAG AGAGAGAGAC CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT 
GGAGAGCGGG TGCGCACTCT GTCAGATTAC CTAAACAGCA ACAAGAAGCG GAAAACAGAA 
GAAAAGGATT TTGGCACAGA CTATGGTAGT GATGCTGACC GCAGCGAAGA TAATTTGGTG 
GTTGATGAGG ATCCTGCTTC TCCTCATAGT GTCCATTCTT ACTCCTCTCG GGAAAATGGT 
GTGGACAAAC CTAGTCTACA GAGAAAAGAC CCTCCACCTG CAAGCCCAAA CTCCATGACC 
TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC AAAGACATTC CCTCTCAGCA AGTGGAGAAG 
GCAGGGACCC CTGGCTTAAA GTCTAGCACT CCTAATTCAC AGAGCGATAT GAATACACCG 
GGCCCAAGTG GAACCAGTGC TTCACAGTTT AGGTCTATCT CCTCCAAACC AGCCATTGAC 
TCATTAGCTC TTGGACTGCG GACTCCATTA GGGGGGCAGG GAGGTTATCC TGCTGCATTT 
AGCATTGCTC ACCCGTCTGT GAATGCAGAT GGTGCTGGTG CCTATGCTGG GTTGCACCTC 
ATGTCTCCAC AAATTAATGG TGCCACTGCA GCTGGAACCT ATGGACGATC TCCTTTAGTC 
AGCTATGACC CCCATGCTCA CATGAGAGGA TTGGGTGCTG CAATTCCAGG GTCTGCATCA 
GGAAAACCGG CATACTCTTT TCATGTCAGT GCAGATGGAC AAATGCAGCC AGTTCCCTTT 
CCCCCAGATG CTCTTATCGG CTCAGGAATT CCACGGCACG CACGTCAGCT CCACGTACTG 
AACCATGGGG AGGTAGTCTG TGCGGTGACA ATAAGCAACT CAACACGCCA TGTCTATACT 
GGAGGCAAAG GCTGTGTTAA AGTGTGGGAT GTTGGCCAAC CGGGTACAAA GACTCCTGTG 
GCACAGCTTG ATTGCCTGAA CCGTGACAAT TACATTCGCT CCTGTAAACT TCTGCCTGAT 
GGACGCAGCC TCATTGTTGG CGGGGAAGCC AGCACCTTGT CCATCTGGGA CTTGGCATCT 
CCTACCCCTA GAATAAAGGC AGAGCTTACA TCCTCAGCCC CAGCCTGCTA CGCTCTTGCT 
ATTAGCCCTG ATGCCAAAGT TTGCTTCTCT TGCTGCTCTG ATGGAAACAT TGTAGTGTGG 
GACCTTCAAA ACCAGACCCT TGTCAGGCAG TTTCAGGGCC ATACGGATGG CGCCAGCTGC 
ATAGATATCT CTCACGATGG TACAAAATTA TGGACTGGGG GATTGGATAA CACAGTGCGA 
TGCTGGGACC TGAGGGAGGG CAGACAGCTC CAACAACATG ACTTCAATTC ACAGATCTTT 
TCTCTGGGGT ATTGCCCCAC TGGGGAATGG CTGGCTGTGG GCATGGAGAG CAGCAATGTT 
GAGGTTCTGC ACGTTTCCAA ACCTGATAAG TACCAACTGC ATCTTCACGA GAGCTGTGTC 
CTGTCCCTGC AATTTGCTTC TTGTGGGAAA TGGTTTGTGA GTACTGGGAA GGACAATTTG 
CTAAATGCTT GGAGGACGCC ATATGGGGCC AGCATATTCC AGTCAAAAGA ATCCTCTTCT 
GTTCTGAGCT GTGACGTTTC CACTGATGAC CAATTCATTG TCACAGGTTC TGGAGACAAA 
AAGGCGACAG TATATGAGGT TATCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961720.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817174.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa27437
Clone ID Related Accession (Same CDS sequence)	XM_012961720.3 , XM_012961720.2
Accession Version	XM_012961720.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGGTACGTTT CATTCCCCCT 
CAACTGACTG TGCACCAGCT GCAGGCCCAG CACCTCTCTC ACCATGCACC TCCAATCCCT 
TTAACCCCAC ATCCATCCAG CCTGCAGCCC CCGGGACTTG CTGCCGCAAC CAGTGCCTCA 
AGCCTATTGG CACTGTCTGG TGTTCTTGGC CTTCAGACAC AACTGGCTGT GAAAGAGGGC 
CTTGCGGATG CAGAACACAG AGAGAGAGAC CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT 
GGAGAGCGGG TGCGCACTCT GTCAGATTAC CTAAACAGCA ACAAGAAGCG GAAAACAGAA 
GAAAAGGATT TTGGCACAGA CTATGGTAGT GATGCTGACC GCAGCGAAGA TAATTTGGTG 
GTTGATGAGG ATCCTGCTTC TCCTCATAGT GTCCATTCTT ACTCCTCTCG GGAAAATGGT 
GTGGACAAAC CTAGTCTACA GAGAAAAGAC CCTCCACCTG CAAGCCCAAA CTCCATGACC 
TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC AAAGACATTC CCTCTCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817174.1
CDS	45..2411
Translation	MREQGKGPIAQTETVPLPPSKMFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGVRFIPPQLTVHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961720.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961720.2

ORF Insert Sequence:

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGGTACGTTT CATTCCCCCT 
CAACTGACTG TGCACCAGCT GCAGGCCCAG CACCTCTCTC ACCATGCACC TCCAATCCCT 
TTAACCCCAC ATCCATCCAG CCTGCAGCCC CCGGGACTTG CTGCCGCAAC CAGTGCCTCA 
AGCCTATTGG CACTGTCTGG TGTTCTTGGC CTTCAGACAC AACTGGCTGT GAAAGAGGGC 
CTTGCGGATG CAGAACACAG AGAGAGAGAC CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT 
GGAGAGCGGG TGCGCACTCT GTCAGATTAC CTAAACAGCA ACAAGAAGCG GAAAACAGAA 
GAAAAGGATT TTGGCACAGA CTATGGTAGT GATGCTGACC GCAGCGAAGA TAATTTGGTG 
GTTGATGAGG ATCCTGCTTC TCCTCATAGT GTCCATTCTT ACTCCTCTCG GGAAAATGGT 
GTGGACAAAC CTAGTCTACA GAGAAAAGAC CCTCCACCTG CAAGCCCAAA CTCCATGACC 
TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC AAAGACATTC CCTCTCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961727.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817181.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa27438
Clone ID Related Accession (Same CDS sequence)	XM_012961727.2 , XM_012961727.3
Accession Version	XM_012961727.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGCACCAGCT GCAGGCCCAG 
CACCTCTCTC ACCATGCACC TCCAATCCCT TTAACCCCAC ATCCATCCAG CCTGCAGCCC 
CCGGGACTTG CTGCCGCAAC CAGTGCCTCA AGCCTATTGG CACTGTCTGG TGTTCTTGGC 
CTTCAGACAC AACTGGCTGT GAAAGAGGGC CTTGCGGATG CAGAACACAG AGAGAGAGAC 
CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT GGAGAGCGGG TGCGCACTCT GTCAGATTAC 
CTAAACAGCA ACAAGAAGCG GAAAACAGAA GAAAAGGATT TTGGCACAGA CTATGGTAGT 
GATGCTGACC GCAGCGAAGA TAATTTGGTG GTTGATGAGG ATCCTGCTTC TCCTCATAGT 
GTCCATTCTT ACTCCTCTCG GGAAAATGGT GTGGACAAAC CTAGTCTACA GAGAAAAGAC 
CCTCCACCTG CAAGCCCAAA CTCCATGACC TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC 
AAAGACATTC CCTCTCAGCA AGTGGAGAAG GCAGGGACCC CTGGCTTAAA GTCTAGCACT 
CCTAATTCAC AGAGCGATAT GAATACACCG GGCCCAAGTG GAACCAGTGC TTCACAGTTT 
AGGTCTATCT CCTCCAAACC AGCCATTGAC TCATTAGCTC TTGGACTGCG GACTCCATTA 
GGGGGGCAGG GAGGTTATCC TGCTGCATTT AGCATTGCTC ACCCGTCTGT GAATGCAGAT 
GGTGCTGGTG CCTATGCTGG GTTGCACCTC ATGTCTCCAC AAATTAATGG TGCCACTGCA 
GCTGGAACCT ATGGACGATC TCCTTTAGTC AGCTATGACC CCCATGCTCA CATGAGAGGA 
TTGGGTGCTG CAATTCCAGG GTCTGCATCA GGAAAACCGG CATACTCTTT TCATGTCAGT 
GCAGATGGAC AAATGCAGCC AGTTCCCTTT CCCCCAGATG CTCTTATCGG CTCAGGAATT 
CCACGGCACG CACGTCAGCT CCACGTACTG AACCATGGGG AGGTAGTCTG TGCGGTGACA 
ATAAGCAACT CAACACGCCA TGTCTATACT GGAGGCAAAG GCTGTGTTAA AGTGTGGGAT 
GTTGGCCAAC CGGGTACAAA GACTCCTGTG GCACAGCTTG ATTGCCTGAA CCGTGACAAT 
TACATTCGCT CCTGTAAACT TCTGCCTGAT GGACGCAGCC TCATTGTTGG CGGGGAAGCC 
AGCACCTTGT CCATCTGGGA CTTGGCATCT CCTACCCCTA GAATAAAGGC AGAGCTTACA 
TCCTCAGCCC CAGCCTGCTA CGCTCTTGCT ATTAGCCCTG ATGCCAAAGT TTGCTTCTCT 
TGCTGCTCTG ATGGAAACAT TGTAGTGTGG GACCTTCAAA ACCAGACCCT TGTCAGGCAG 
TTTCAGGGCC ATACGGATGG CGCCAGCTGC ATAGATATCT CTCACGATGG TACAAAATTA 
TGGACTGGGG GATTGGATAA CACAGTGCGA TGCTGGGACC TGAGGGAGGG CAGACAGCTC 
CAACAACATG ACTTCAATTC ACAGATCTTT TCTCTGGGGT ATTGCCCCAC TGGGGAATGG 
CTGGCTGTGG GCATGGAGAG CAGCAATGTT GAGGTTCTGC ACGTTTCCAA ACCTGATAAG 
TACCAACTGC ATCTTCACGA GAGCTGTGTC CTGTCCCTGC AATTTGCTTC TTGTGGGAAA 
TGGTTTGTGA GTACTGGGAA GGACAATTTG CTAAATGCTT GGAGGACGCC ATATGGGGCC 
AGCATATTCC AGTCAAAAGA ATCCTCTTCT GTTCTGAGCT GTGACGTTTC CACTGATGAC 
CAATTCATTG TCACAGGTTC TGGAGACAAA AAGGCGACAG TATATGAGGT TATCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817181.1
CDS	45..2384
Translation	MREQGKGPIAQTETVPLPPSKMFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961727.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961727.2

ORF Insert Sequence:

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGCACCAGCT GCAGGCCCAG 
CACCTCTCTC ACCATGCACC TCCAATCCCT TTAACCCCAC ATCCATCCAG CCTGCAGCCC 
CCGGGACTTG CTGCCGCAAC CAGTGCCTCA AGCCTATTGG CACTGTCTGG TGTTCTTGGC 
CTTCAGACAC AACTGGCTGT GAAAGAGGGC CTTGCGGATG CAGAACACAG AGAGAGAGAC 
CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT GGAGAGCGGG TGCGCACTCT GTCAGATTAC 
CTAAACAGCA ACAAGAAGCG GAAAACAGAA GAAAAGGATT TTGGCACAGA CTATGGTAGT 
GATGCTGACC GCAGCGAAGA TAATTTGGTG GTTGATGAGG ATCCTGCTTC TCCTCATAGT 
GTCCATTCTT ACTCCTCTCG GGAAAATGGT GTGGACAAAC CTAGTCTACA GAGAAAAGAC 
CCTCCACCTG CAAGCCCAAA CTCCATGACC TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC 
AAAGACATTC CCTCTCAGCA AGTGGAGAAG GCAGGGACCC CTGGCTTAAA GTCTAGCACT 
CCTAATTCAC AGAGCGATAT GAATACACCG GGCCCAAGTG GAACCAGTGC TTCACAGTTT 
AGGTCTATCT CCTCCAAACC AGCCATTGAC TCATTAGCTC TTGGACTGCG GACTCCATTA 
GGGGGGCAGG GAGGTTATCC TGCTGCATTT AGCATTGCTC ACCCGTCTGT GAATGCAGAT 
GGTGCTGGTG CCTATGCTGG GTTGCACCTC ATGTCTCCAC AAATTAATGG TGCCACTGCA 
GCTGGAACCT ATGGACGATC TCCTTTAGTC AGCTATGACC CCCATGCTCA CATGAGAGGA 
TTGGGTGCTG CAATTCCAGG GTCTGCATCA GGAAAACCGG CATACTCTTT TCATGTCAGT 
GCAGATGGAC AAATGCAGCC AGTTCCCTTT CCCCCAGATG CTCTTATCGG CTCAGGAATT 
CCACGGCACG CACGTCAGCT CCACGTACTG AACCATGGGG AGGTAGTCTG TGCGGTGACA 
ATAAGCAACT CAACACGCCA TGTCTATACT GGAGGCAAAG GCTGTGTTAA AGTGTGGGAT 
GTTGGCCAAC CGGGTACAAA GACTCCTGTG GCACAGCTTG ATTGCCTGAA CCGTGACAAT 
TACATTCGCT CCTGTAAACT TCTGCCTGAT GGACGCAGCC TCATTGTTGG CGGGGAAGCC 
AGCACCTTGT CCATCTGGGA CTTGGCATCT CCTACCCCTA GAATAAAGGC AGAGCTTACA 
TCCTCAGCCC CAGCCTGCTA CGCTCTTGCT ATTAGCCCTG ATGCCAAAGT TTGCTTCTCT 
TGCTGCTCTG ATGGAAACAT TGTAGTGTGG GACCTTCAAA ACCAGACCCT TGTCAGGCAG 
TTTCAGGGCC ATACGGATGG CGCCAGCTGC ATAGATATCT CTCACGATGG TACAAAATTA 
TGGACTGGGG GATTGGATAA CACAGTGCGA TGCTGGGACC TGAGGGAGGG CAGACAGCTC 
CAACAACATG ACTTCAATTC ACAGATCTTT TCTCTGGGGT ATTGCCCCAC TGGGGAATGG 
CTGGCTGTGG GCATGGAGAG CAGCAATGTT GAGGTTCTGC ACGTTTCCAA ACCTGATAAG 
TACCAACTGC ATCTTCACGA GAGCTGTGTC CTGTCCCTGC AATTTGCTTC TTGTGGGAAA 
TGGTTTGTGA GTACTGGGAA GGACAATTTG CTAAATGCTT GGAGGACGCC ATATGGGGCC 
AGCATATTCC AGTCAAAAGA ATCCTCTTCT GTTCTGAGCT GTGACGTTTC CACTGATGAC 
CAATTCATTG TCACAGGTTC TGGAGACAAA AAGGCGACAG TATATGAGGT TATCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961733.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817187.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa27439
Clone ID Related Accession (Same CDS sequence)	XM_012961733.3 , XM_012961733.2
Accession Version	XM_012961733.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGCACCAGCT GCAGGCCCAG 
CACCTCTCTC ACCATGCACC TCCAATCCCT TTAACCCCAC ATCCATCCAG CCTGCAGCCC 
CCGGGACTTG CTGCCGCAAC CAGTGCCTCA AGCCTATTGG CACTGTCTGG TGTTCTTGGC 
CTTCAGACAC AACTGGCTGT GAAAGAGGGC CTTGCGGATG CAGAACACAG AGAGAGAGAC 
CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT GGAGAGCGGG TGCGCACTCT GTCAGATTAC 
CTAAACAGCA ACAAGAAGCG GAAAACAGAA GAAAAGGATT TTGGCACAGA CTATGGTAGT 
GATGCTGACC GCAGCGAAGA TAATTTGGTG GTTGATGAGG ATCCTGCTTC TCCTCATAGT 
GTCCATTCTT ACTCCTCTCG GGAAAATGGT GTGGACAAAC CTAGTCTACA GAGAAAAGAC 
CCTCCACCTG CAAGCCCAAA CTCCATGACC TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC 
AAAGACATTC CCTCTCAAGT GGAGAAGGCA GGGACCCCTG GCTTAAAGTC TAGCACTCCT 
AATTCACAGA GCGATATGAA TACACCGGGC CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG 
TCTATCTCCT CCAAACCAGC CATTGACTCA TTAGCTCTTG GACTGCGGAC TCCATTAGGG 
GGGCAGGGAG GTTATCCTGC TGCATTTAGC ATTGCTCACC CGTCTGTGAA TGCAGATGGT 
GCTGGTGCCT ATGCTGGGTT GCACCTCATG TCTCCACAAA TTAATGGTGC CACTGCAGCT 
GGAACCTATG GACGATCTCC TTTAGTCAGC TATGACCCCC ATGCTCACAT GAGAGGATTG 
GGTGCTGCAA TTCCAGGGTC TGCATCAGGA AAACCGGCAT ACTCTTTTCA TGTCAGTGCA 
GATGGACAAA TGCAGCCAGT TCCCTTTCCC CCAGATGCTC TTATCGGCTC AGGAATTCCA 
CGGCACGCAC GTCAGCTCCA CGTACTGAAC CATGGGGAGG TAGTCTGTGC GGTGACAATA 
AGCAACTCAA CACGCCATGT CTATACTGGA GGCAAAGGCT GTGTTAAAGT GTGGGATGTT 
GGCCAACCGG GTACAAAGAC TCCTGTGGCA CAGCTTGATT GCCTGAACCG TGACAATTAC 
ATTCGCTCCT GTAAACTTCT GCCTGATGGA CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC 
ACCTTGTCCA TCTGGGACTT GGCATCTCCT ACCCCTAGAA TAAAGGCAGA GCTTACATCC 
TCAGCCCCAG CCTGCTACGC TCTTGCTATT AGCCCTGATG CCAAAGTTTG CTTCTCTTGC 
TGCTCTGATG GAAACATTGT AGTGTGGGAC CTTCAAAACC AGACCCTTGT CAGGCAGTTT 
CAGGGCCATA CGGATGGCGC CAGCTGCATA GATATCTCTC ACGATGGTAC AAAATTATGG 
ACTGGGGGAT TGGATAACAC AGTGCGATGC TGGGACCTGA GGGAGGGCAG ACAGCTCCAA 
CAACATGACT TCAATTCACA GATCTTTTCT CTGGGGTATT GCCCCACTGG GGAATGGCTG 
GCTGTGGGCA TGGAGAGCAG CAATGTTGAG GTTCTGCACG TTTCCAAACC TGATAAGTAC 
CAACTGCATC TTCACGAGAG CTGTGTCCTG TCCCTGCAAT TTGCTTCTTG TGGGAAATGG 
TTTGTGAGTA CTGGGAAGGA CAATTTGCTA AATGCTTGGA GGACGCCATA TGGGGCCAGC 
ATATTCCAGT CAAAAGAATC CTCTTCTGTT CTGAGCTGTG ACGTTTCCAC TGATGACCAA 
TTCATTGTCA CAGGTTCTGG AGACAAAAAG GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817187.1
CDS	46..2382
Translation	MREQGKGPIAQTETVPLPPSKMFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961733.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961733.2

ORF Insert Sequence:

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGCACCAGCT GCAGGCCCAG 
CACCTCTCTC ACCATGCACC TCCAATCCCT TTAACCCCAC ATCCATCCAG CCTGCAGCCC 
CCGGGACTTG CTGCCGCAAC CAGTGCCTCA AGCCTATTGG CACTGTCTGG TGTTCTTGGC 
CTTCAGACAC AACTGGCTGT GAAAGAGGGC CTTGCGGATG CAGAACACAG AGAGAGAGAC 
CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT GGAGAGCGGG TGCGCACTCT GTCAGATTAC 
CTAAACAGCA ACAAGAAGCG GAAAACAGAA GAAAAGGATT TTGGCACAGA CTATGGTAGT 
GATGCTGACC GCAGCGAAGA TAATTTGGTG GTTGATGAGG ATCCTGCTTC TCCTCATAGT 
GTCCATTCTT ACTCCTCTCG GGAAAATGGT GTGGACAAAC CTAGTCTACA GAGAAAAGAC 
CCTCCACCTG CAAGCCCAAA CTCCATGACC TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC 
AAAGACATTC CCTCTCAAGT GGAGAAGGCA GGGACCCCTG GCTTAAAGTC TAGCACTCCT 
AATTCACAGA GCGATATGAA TACACCGGGC CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG 
TCTATCTCCT CCAAACCAGC CATTGACTCA TTAGCTCTTG GACTGCGGAC TCCATTAGGG 
GGGCAGGGAG GTTATCCTGC TGCATTTAGC ATTGCTCACC CGTCTGTGAA TGCAGATGGT 
GCTGGTGCCT ATGCTGGGTT GCACCTCATG TCTCCACAAA TTAATGGTGC CACTGCAGCT 
GGAACCTATG GACGATCTCC TTTAGTCAGC TATGACCCCC ATGCTCACAT GAGAGGATTG 
GGTGCTGCAA TTCCAGGGTC TGCATCAGGA AAACCGGCAT ACTCTTTTCA TGTCAGTGCA 
GATGGACAAA TGCAGCCAGT TCCCTTTCCC CCAGATGCTC TTATCGGCTC AGGAATTCCA 
CGGCACGCAC GTCAGCTCCA CGTACTGAAC CATGGGGAGG TAGTCTGTGC GGTGACAATA 
AGCAACTCAA CACGCCATGT CTATACTGGA GGCAAAGGCT GTGTTAAAGT GTGGGATGTT 
GGCCAACCGG GTACAAAGAC TCCTGTGGCA CAGCTTGATT GCCTGAACCG TGACAATTAC 
ATTCGCTCCT GTAAACTTCT GCCTGATGGA CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC 
ACCTTGTCCA TCTGGGACTT GGCATCTCCT ACCCCTAGAA TAAAGGCAGA GCTTACATCC 
TCAGCCCCAG CCTGCTACGC TCTTGCTATT AGCCCTGATG CCAAAGTTTG CTTCTCTTGC 
TGCTCTGATG GAAACATTGT AGTGTGGGAC CTTCAAAACC AGACCCTTGT CAGGCAGTTT 
CAGGGCCATA CGGATGGCGC CAGCTGCATA GATATCTCTC ACGATGGTAC AAAATTATGG 
ACTGGGGGAT TGGATAACAC AGTGCGATGC TGGGACCTGA GGGAGGGCAG ACAGCTCCAA 
CAACATGACT TCAATTCACA GATCTTTTCT CTGGGGTATT GCCCCACTGG GGAATGGCTG 
GCTGTGGGCA TGGAGAGCAG CAATGTTGAG GTTCTGCACG TTTCCAAACC TGATAAGTAC 
CAACTGCATC TTCACGAGAG CTGTGTCCTG TCCCTGCAAT TTGCTTCTTG TGGGAAATGG 
TTTGTGAGTA CTGGGAAGGA CAATTTGCTA AATGCTTGGA GGACGCCATA TGGGGCCAGC 
ATATTCCAGT CAAAAGAATC CTCTTCTGTT CTGAGCTGTG ACGTTTCCAC TGATGACCAA 
TTCATTGTCA CAGGTTCTGG AGACAAAAAG GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961741.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817195.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X6, mRNA.

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CloneID	OXa27440
Clone ID Related Accession (Same CDS sequence)	XM_012961741.2 , XM_012961741.3 , XM_012961745.2 , XM_012961736.2 , XM_012961736.3
Accession Version	XM_012961741.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGG TACGTTTCAT TCCCCCTCAA 
CTGACTGTGC ACCAGCTGCA GGCCCAGCAC CTCTCTCACC ATGCACCTCC AATCCCTTTA 
ACCCCACATC CATCCAGCCT GCAGCCCCCG GGACTTGCTG CCGCAACCAG TGCCTCAAGC 
CTATTGGCAC TGTCTGGTGT TCTTGGCCTT CAGACACAAC TGGCTGTGAA AGAGGGCCTT 
GCGGATGCAG AACACAGAGA GAGAGACCCA GGGCCTAGCT GTGTAACTCT TCCTAATGGA 
GAGCGGGTGC GCACTCTGTC AGATTACCTA AACAGCAACA AGAAGCGGAA AACAGAAGAA 
AAGGATTTTG GCACAGACTA TGGTAGTGAT GCTGACCGCA GCGAAGATAA TTTGGTGGTT 
GATGAGGATC CTGCTTCTCC TCATAGTGTC CATTCTTACT CCTCTCGGGA AAATGGTGTG 
GACAAACCTA GTCTACAGAG AAAAGACCCT CCACCTGCAA GCCCAAACTC CATGACCTCT 
TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA GACATTCCCT CTCAGCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817195.1
CDS	129..2435
Translation	MFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGVRFIPPQLTVHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961741.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961741.2

ORF Insert Sequence:

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGG TACGTTTCAT TCCCCCTCAA 
CTGACTGTGC ACCAGCTGCA GGCCCAGCAC CTCTCTCACC ATGCACCTCC AATCCCTTTA 
ACCCCACATC CATCCAGCCT GCAGCCCCCG GGACTTGCTG CCGCAACCAG TGCCTCAAGC 
CTATTGGCAC TGTCTGGTGT TCTTGGCCTT CAGACACAAC TGGCTGTGAA AGAGGGCCTT 
GCGGATGCAG AACACAGAGA GAGAGACCCA GGGCCTAGCT GTGTAACTCT TCCTAATGGA 
GAGCGGGTGC GCACTCTGTC AGATTACCTA AACAGCAACA AGAAGCGGAA AACAGAAGAA 
AAGGATTTTG GCACAGACTA TGGTAGTGAT GCTGACCGCA GCGAAGATAA TTTGGTGGTT 
GATGAGGATC CTGCTTCTCC TCATAGTGTC CATTCTTACT CCTCTCGGGA AAATGGTGTG 
GACAAACCTA GTCTACAGAG AAAAGACCCT CCACCTGCAA GCCCAAACTC CATGACCTCT 
TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA GACATTCCCT CTCAGCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961745.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817199.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X7, mRNA.

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CloneID	OXa27440
Clone ID Related Accession (Same CDS sequence)	XM_012961741.2 , XM_012961741.3 , XM_012961745.2 , XM_012961736.2 , XM_012961736.3
Accession Version	XM_012961745.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGG TACGTTTCAT TCCCCCTCAA 
CTGACTGTGC ACCAGCTGCA GGCCCAGCAC CTCTCTCACC ATGCACCTCC AATCCCTTTA 
ACCCCACATC CATCCAGCCT GCAGCCCCCG GGACTTGCTG CCGCAACCAG TGCCTCAAGC 
CTATTGGCAC TGTCTGGTGT TCTTGGCCTT CAGACACAAC TGGCTGTGAA AGAGGGCCTT 
GCGGATGCAG AACACAGAGA GAGAGACCCA GGGCCTAGCT GTGTAACTCT TCCTAATGGA 
GAGCGGGTGC GCACTCTGTC AGATTACCTA AACAGCAACA AGAAGCGGAA AACAGAAGAA 
AAGGATTTTG GCACAGACTA TGGTAGTGAT GCTGACCGCA GCGAAGATAA TTTGGTGGTT 
GATGAGGATC CTGCTTCTCC TCATAGTGTC CATTCTTACT CCTCTCGGGA AAATGGTGTG 
GACAAACCTA GTCTACAGAG AAAAGACCCT CCACCTGCAA GCCCAAACTC CATGACCTCT 
TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA GACATTCCCT CTCAGCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817199.1
CDS	220..2526
Translation	MFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGVRFIPPQLTVHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961745.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961745.2

ORF Insert Sequence:

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGG TACGTTTCAT TCCCCCTCAA 
CTGACTGTGC ACCAGCTGCA GGCCCAGCAC CTCTCTCACC ATGCACCTCC AATCCCTTTA 
ACCCCACATC CATCCAGCCT GCAGCCCCCG GGACTTGCTG CCGCAACCAG TGCCTCAAGC 
CTATTGGCAC TGTCTGGTGT TCTTGGCCTT CAGACACAAC TGGCTGTGAA AGAGGGCCTT 
GCGGATGCAG AACACAGAGA GAGAGACCCA GGGCCTAGCT GTGTAACTCT TCCTAATGGA 
GAGCGGGTGC GCACTCTGTC AGATTACCTA AACAGCAACA AGAAGCGGAA AACAGAAGAA 
AAGGATTTTG GCACAGACTA TGGTAGTGAT GCTGACCGCA GCGAAGATAA TTTGGTGGTT 
GATGAGGATC CTGCTTCTCC TCATAGTGTC CATTCTTACT CCTCTCGGGA AAATGGTGTG 
GACAAACCTA GTCTACAGAG AAAAGACCCT CCACCTGCAA GCCCAAACTC CATGACCTCT 
TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA GACATTCCCT CTCAGCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961736.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817190.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X5, mRNA.

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CloneID	OXa27440
Clone ID Related Accession (Same CDS sequence)	XM_012961741.2 , XM_012961741.3 , XM_012961745.2 , XM_012961736.2 , XM_012961736.3
Accession Version	XM_012961736.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961736.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGG TACGTTTCAT TCCCCCTCAA 
CTGACTGTGC ACCAGCTGCA GGCCCAGCAC CTCTCTCACC ATGCACCTCC AATCCCTTTA 
ACCCCACATC CATCCAGCCT GCAGCCCCCG GGACTTGCTG CCGCAACCAG TGCCTCAAGC 
CTATTGGCAC TGTCTGGTGT TCTTGGCCTT CAGACACAAC TGGCTGTGAA AGAGGGCCTT 
GCGGATGCAG AACACAGAGA GAGAGACCCA GGGCCTAGCT GTGTAACTCT TCCTAATGGA 
GAGCGGGTGC GCACTCTGTC AGATTACCTA AACAGCAACA AGAAGCGGAA AACAGAAGAA 
AAGGATTTTG GCACAGACTA TGGTAGTGAT GCTGACCGCA GCGAAGATAA TTTGGTGGTT 
GATGAGGATC CTGCTTCTCC TCATAGTGTC CATTCTTACT CCTCTCGGGA AAATGGTGTG 
GACAAACCTA GTCTACAGAG AAAAGACCCT CCACCTGCAA GCCCAAACTC CATGACCTCT 
TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA GACATTCCCT CTCAGCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817190.1
CDS	231..2537
Translation	MFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGVRFIPPQLTVHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961736.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961736.2

ORF Insert Sequence:

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGG TACGTTTCAT TCCCCCTCAA 
CTGACTGTGC ACCAGCTGCA GGCCCAGCAC CTCTCTCACC ATGCACCTCC AATCCCTTTA 
ACCCCACATC CATCCAGCCT GCAGCCCCCG GGACTTGCTG CCGCAACCAG TGCCTCAAGC 
CTATTGGCAC TGTCTGGTGT TCTTGGCCTT CAGACACAAC TGGCTGTGAA AGAGGGCCTT 
GCGGATGCAG AACACAGAGA GAGAGACCCA GGGCCTAGCT GTGTAACTCT TCCTAATGGA 
GAGCGGGTGC GCACTCTGTC AGATTACCTA AACAGCAACA AGAAGCGGAA AACAGAAGAA 
AAGGATTTTG GCACAGACTA TGGTAGTGAT GCTGACCGCA GCGAAGATAA TTTGGTGGTT 
GATGAGGATC CTGCTTCTCC TCATAGTGTC CATTCTTACT CCTCTCGGGA AAATGGTGTG 
GACAAACCTA GTCTACAGAG AAAAGACCCT CCACCTGCAA GCCCAAACTC CATGACCTCT 
TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA GACATTCCCT CTCAGCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961752.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817206.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X8, mRNA.

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CloneID	OXa27441
Clone ID Related Accession (Same CDS sequence)	XM_012961752.2 , XM_012961756.2 , XM_012961752.3
Accession Version	XM_012961752.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961752.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGC ACCAGCTGCA GGCCCAGCAC 
CTCTCTCACC ATGCACCTCC AATCCCTTTA ACCCCACATC CATCCAGCCT GCAGCCCCCG 
GGACTTGCTG CCGCAACCAG TGCCTCAAGC CTATTGGCAC TGTCTGGTGT TCTTGGCCTT 
CAGACACAAC TGGCTGTGAA AGAGGGCCTT GCGGATGCAG AACACAGAGA GAGAGACCCA 
GGGCCTAGCT GTGTAACTCT TCCTAATGGA GAGCGGGTGC GCACTCTGTC AGATTACCTA 
AACAGCAACA AGAAGCGGAA AACAGAAGAA AAGGATTTTG GCACAGACTA TGGTAGTGAT 
GCTGACCGCA GCGAAGATAA TTTGGTGGTT GATGAGGATC CTGCTTCTCC TCATAGTGTC 
CATTCTTACT CCTCTCGGGA AAATGGTGTG GACAAACCTA GTCTACAGAG AAAAGACCCT 
CCACCTGCAA GCCCAAACTC CATGACCTCT TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA 
GACATTCCCT CTCAGCAAGT GGAGAAGGCA GGGACCCCTG GCTTAAAGTC TAGCACTCCT 
AATTCACAGA GCGATATGAA TACACCGGGC CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG 
TCTATCTCCT CCAAACCAGC CATTGACTCA TTAGCTCTTG GACTGCGGAC TCCATTAGGG 
GGGCAGGGAG GTTATCCTGC TGCATTTAGC ATTGCTCACC CGTCTGTGAA TGCAGATGGT 
GCTGGTGCCT ATGCTGGGTT GCACCTCATG TCTCCACAAA TTAATGGTGC CACTGCAGCT 
GGAACCTATG GACGATCTCC TTTAGTCAGC TATGACCCCC ATGCTCACAT GAGAGGATTG 
GGTGCTGCAA TTCCAGGGTC TGCATCAGGA AAACCGGCAT ACTCTTTTCA TGTCAGTGCA 
GATGGACAAA TGCAGCCAGT TCCCTTTCCC CCAGATGCTC TTATCGGCTC AGGAATTCCA 
CGGCACGCAC GTCAGCTCCA CGTACTGAAC CATGGGGAGG TAGTCTGTGC GGTGACAATA 
AGCAACTCAA CACGCCATGT CTATACTGGA GGCAAAGGCT GTGTTAAAGT GTGGGATGTT 
GGCCAACCGG GTACAAAGAC TCCTGTGGCA CAGCTTGATT GCCTGAACCG TGACAATTAC 
ATTCGCTCCT GTAAACTTCT GCCTGATGGA CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC 
ACCTTGTCCA TCTGGGACTT GGCATCTCCT ACCCCTAGAA TAAAGGCAGA GCTTACATCC 
TCAGCCCCAG CCTGCTACGC TCTTGCTATT AGCCCTGATG CCAAAGTTTG CTTCTCTTGC 
TGCTCTGATG GAAACATTGT AGTGTGGGAC CTTCAAAACC AGACCCTTGT CAGGCAGTTT 
CAGGGCCATA CGGATGGCGC CAGCTGCATA GATATCTCTC ACGATGGTAC AAAATTATGG 
ACTGGGGGAT TGGATAACAC AGTGCGATGC TGGGACCTGA GGGAGGGCAG ACAGCTCCAA 
CAACATGACT TCAATTCACA GATCTTTTCT CTGGGGTATT GCCCCACTGG GGAATGGCTG 
GCTGTGGGCA TGGAGAGCAG CAATGTTGAG GTTCTGCACG TTTCCAAACC TGATAAGTAC 
CAACTGCATC TTCACGAGAG CTGTGTCCTG TCCCTGCAAT TTGCTTCTTG TGGGAAATGG 
TTTGTGAGTA CTGGGAAGGA CAATTTGCTA AATGCTTGGA GGACGCCATA TGGGGCCAGC 
ATATTCCAGT CAAAAGAATC CTCTTCTGTT CTGAGCTGTG ACGTTTCCAC TGATGACCAA 
TTCATTGTCA CAGGTTCTGG AGACAAAAAG GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817206.1
CDS	231..2507
Translation	MFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961752.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961752.2

ORF Insert Sequence:

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGC ACCAGCTGCA GGCCCAGCAC 
CTCTCTCACC ATGCACCTCC AATCCCTTTA ACCCCACATC CATCCAGCCT GCAGCCCCCG 
GGACTTGCTG CCGCAACCAG TGCCTCAAGC CTATTGGCAC TGTCTGGTGT TCTTGGCCTT 
CAGACACAAC TGGCTGTGAA AGAGGGCCTT GCGGATGCAG AACACAGAGA GAGAGACCCA 
GGGCCTAGCT GTGTAACTCT TCCTAATGGA GAGCGGGTGC GCACTCTGTC AGATTACCTA 
AACAGCAACA AGAAGCGGAA AACAGAAGAA AAGGATTTTG GCACAGACTA TGGTAGTGAT 
GCTGACCGCA GCGAAGATAA TTTGGTGGTT GATGAGGATC CTGCTTCTCC TCATAGTGTC 
CATTCTTACT CCTCTCGGGA AAATGGTGTG GACAAACCTA GTCTACAGAG AAAAGACCCT 
CCACCTGCAA GCCCAAACTC CATGACCTCT TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA 
GACATTCCCT CTCAGCAAGT GGAGAAGGCA GGGACCCCTG GCTTAAAGTC TAGCACTCCT 
AATTCACAGA GCGATATGAA TACACCGGGC CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG 
TCTATCTCCT CCAAACCAGC CATTGACTCA TTAGCTCTTG GACTGCGGAC TCCATTAGGG 
GGGCAGGGAG GTTATCCTGC TGCATTTAGC ATTGCTCACC CGTCTGTGAA TGCAGATGGT 
GCTGGTGCCT ATGCTGGGTT GCACCTCATG TCTCCACAAA TTAATGGTGC CACTGCAGCT 
GGAACCTATG GACGATCTCC TTTAGTCAGC TATGACCCCC ATGCTCACAT GAGAGGATTG 
GGTGCTGCAA TTCCAGGGTC TGCATCAGGA AAACCGGCAT ACTCTTTTCA TGTCAGTGCA 
GATGGACAAA TGCAGCCAGT TCCCTTTCCC CCAGATGCTC TTATCGGCTC AGGAATTCCA 
CGGCACGCAC GTCAGCTCCA CGTACTGAAC CATGGGGAGG TAGTCTGTGC GGTGACAATA 
AGCAACTCAA CACGCCATGT CTATACTGGA GGCAAAGGCT GTGTTAAAGT GTGGGATGTT 
GGCCAACCGG GTACAAAGAC TCCTGTGGCA CAGCTTGATT GCCTGAACCG TGACAATTAC 
ATTCGCTCCT GTAAACTTCT GCCTGATGGA CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC 
ACCTTGTCCA TCTGGGACTT GGCATCTCCT ACCCCTAGAA TAAAGGCAGA GCTTACATCC 
TCAGCCCCAG CCTGCTACGC TCTTGCTATT AGCCCTGATG CCAAAGTTTG CTTCTCTTGC 
TGCTCTGATG GAAACATTGT AGTGTGGGAC CTTCAAAACC AGACCCTTGT CAGGCAGTTT 
CAGGGCCATA CGGATGGCGC CAGCTGCATA GATATCTCTC ACGATGGTAC AAAATTATGG 
ACTGGGGGAT TGGATAACAC AGTGCGATGC TGGGACCTGA GGGAGGGCAG ACAGCTCCAA 
CAACATGACT TCAATTCACA GATCTTTTCT CTGGGGTATT GCCCCACTGG GGAATGGCTG 
GCTGTGGGCA TGGAGAGCAG CAATGTTGAG GTTCTGCACG TTTCCAAACC TGATAAGTAC 
CAACTGCATC TTCACGAGAG CTGTGTCCTG TCCCTGCAAT TTGCTTCTTG TGGGAAATGG 
TTTGTGAGTA CTGGGAAGGA CAATTTGCTA AATGCTTGGA GGACGCCATA TGGGGCCAGC 
ATATTCCAGT CAAAAGAATC CTCTTCTGTT CTGAGCTGTG ACGTTTCCAC TGATGACCAA 
TTCATTGTCA CAGGTTCTGG AGACAAAAAG GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961756.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817210.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X9, mRNA.

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CloneID	OXa27441
Clone ID Related Accession (Same CDS sequence)	XM_012961752.2 , XM_012961756.2 , XM_012961752.3
Accession Version	XM_012961756.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGC ACCAGCTGCA GGCCCAGCAC 
CTCTCTCACC ATGCACCTCC AATCCCTTTA ACCCCACATC CATCCAGCCT GCAGCCCCCG 
GGACTTGCTG CCGCAACCAG TGCCTCAAGC CTATTGGCAC TGTCTGGTGT TCTTGGCCTT 
CAGACACAAC TGGCTGTGAA AGAGGGCCTT GCGGATGCAG AACACAGAGA GAGAGACCCA 
GGGCCTAGCT GTGTAACTCT TCCTAATGGA GAGCGGGTGC GCACTCTGTC AGATTACCTA 
AACAGCAACA AGAAGCGGAA AACAGAAGAA AAGGATTTTG GCACAGACTA TGGTAGTGAT 
GCTGACCGCA GCGAAGATAA TTTGGTGGTT GATGAGGATC CTGCTTCTCC TCATAGTGTC 
CATTCTTACT CCTCTCGGGA AAATGGTGTG GACAAACCTA GTCTACAGAG AAAAGACCCT 
CCACCTGCAA GCCCAAACTC CATGACCTCT TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA 
GACATTCCCT CTCAGCAAGT GGAGAAGGCA GGGACCCCTG GCTTAAAGTC TAGCACTCCT 
AATTCACAGA GCGATATGAA TACACCGGGC CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG 
TCTATCTCCT CCAAACCAGC CATTGACTCA TTAGCTCTTG GACTGCGGAC TCCATTAGGG 
GGGCAGGGAG GTTATCCTGC TGCATTTAGC ATTGCTCACC CGTCTGTGAA TGCAGATGGT 
GCTGGTGCCT ATGCTGGGTT GCACCTCATG TCTCCACAAA TTAATGGTGC CACTGCAGCT 
GGAACCTATG GACGATCTCC TTTAGTCAGC TATGACCCCC ATGCTCACAT GAGAGGATTG 
GGTGCTGCAA TTCCAGGGTC TGCATCAGGA AAACCGGCAT ACTCTTTTCA TGTCAGTGCA 
GATGGACAAA TGCAGCCAGT TCCCTTTCCC CCAGATGCTC TTATCGGCTC AGGAATTCCA 
CGGCACGCAC GTCAGCTCCA CGTACTGAAC CATGGGGAGG TAGTCTGTGC GGTGACAATA 
AGCAACTCAA CACGCCATGT CTATACTGGA GGCAAAGGCT GTGTTAAAGT GTGGGATGTT 
GGCCAACCGG GTACAAAGAC TCCTGTGGCA CAGCTTGATT GCCTGAACCG TGACAATTAC 
ATTCGCTCCT GTAAACTTCT GCCTGATGGA CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC 
ACCTTGTCCA TCTGGGACTT GGCATCTCCT ACCCCTAGAA TAAAGGCAGA GCTTACATCC 
TCAGCCCCAG CCTGCTACGC TCTTGCTATT AGCCCTGATG CCAAAGTTTG CTTCTCTTGC 
TGCTCTGATG GAAACATTGT AGTGTGGGAC CTTCAAAACC AGACCCTTGT CAGGCAGTTT 
CAGGGCCATA CGGATGGCGC CAGCTGCATA GATATCTCTC ACGATGGTAC AAAATTATGG 
ACTGGGGGAT TGGATAACAC AGTGCGATGC TGGGACCTGA GGGAGGGCAG ACAGCTCCAA 
CAACATGACT TCAATTCACA GATCTTTTCT CTGGGGTATT GCCCCACTGG GGAATGGCTG 
GCTGTGGGCA TGGAGAGCAG CAATGTTGAG GTTCTGCACG TTTCCAAACC TGATAAGTAC 
CAACTGCATC TTCACGAGAG CTGTGTCCTG TCCCTGCAAT TTGCTTCTTG TGGGAAATGG 
TTTGTGAGTA CTGGGAAGGA CAATTTGCTA AATGCTTGGA GGACGCCATA TGGGGCCAGC 
ATATTCCAGT CAAAAGAATC CTCTTCTGTT CTGAGCTGTG ACGTTTCCAC TGATGACCAA 
TTCATTGTCA CAGGTTCTGG AGACAAAAAG GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817210.1
CDS	203..2479
Translation	MFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961756.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961756.2

ORF Insert Sequence:

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGC ACCAGCTGCA GGCCCAGCAC 
CTCTCTCACC ATGCACCTCC AATCCCTTTA ACCCCACATC CATCCAGCCT GCAGCCCCCG 
GGACTTGCTG CCGCAACCAG TGCCTCAAGC CTATTGGCAC TGTCTGGTGT TCTTGGCCTT 
CAGACACAAC TGGCTGTGAA AGAGGGCCTT GCGGATGCAG AACACAGAGA GAGAGACCCA 
GGGCCTAGCT GTGTAACTCT TCCTAATGGA GAGCGGGTGC GCACTCTGTC AGATTACCTA 
AACAGCAACA AGAAGCGGAA AACAGAAGAA AAGGATTTTG GCACAGACTA TGGTAGTGAT 
GCTGACCGCA GCGAAGATAA TTTGGTGGTT GATGAGGATC CTGCTTCTCC TCATAGTGTC 
CATTCTTACT CCTCTCGGGA AAATGGTGTG GACAAACCTA GTCTACAGAG AAAAGACCCT 
CCACCTGCAA GCCCAAACTC CATGACCTCT TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA 
GACATTCCCT CTCAGCAAGT GGAGAAGGCA GGGACCCCTG GCTTAAAGTC TAGCACTCCT 
AATTCACAGA GCGATATGAA TACACCGGGC CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG 
TCTATCTCCT CCAAACCAGC CATTGACTCA TTAGCTCTTG GACTGCGGAC TCCATTAGGG 
GGGCAGGGAG GTTATCCTGC TGCATTTAGC ATTGCTCACC CGTCTGTGAA TGCAGATGGT 
GCTGGTGCCT ATGCTGGGTT GCACCTCATG TCTCCACAAA TTAATGGTGC CACTGCAGCT 
GGAACCTATG GACGATCTCC TTTAGTCAGC TATGACCCCC ATGCTCACAT GAGAGGATTG 
GGTGCTGCAA TTCCAGGGTC TGCATCAGGA AAACCGGCAT ACTCTTTTCA TGTCAGTGCA 
GATGGACAAA TGCAGCCAGT TCCCTTTCCC CCAGATGCTC TTATCGGCTC AGGAATTCCA 
CGGCACGCAC GTCAGCTCCA CGTACTGAAC CATGGGGAGG TAGTCTGTGC GGTGACAATA 
AGCAACTCAA CACGCCATGT CTATACTGGA GGCAAAGGCT GTGTTAAAGT GTGGGATGTT 
GGCCAACCGG GTACAAAGAC TCCTGTGGCA CAGCTTGATT GCCTGAACCG TGACAATTAC 
ATTCGCTCCT GTAAACTTCT GCCTGATGGA CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC 
ACCTTGTCCA TCTGGGACTT GGCATCTCCT ACCCCTAGAA TAAAGGCAGA GCTTACATCC 
TCAGCCCCAG CCTGCTACGC TCTTGCTATT AGCCCTGATG CCAAAGTTTG CTTCTCTTGC 
TGCTCTGATG GAAACATTGT AGTGTGGGAC CTTCAAAACC AGACCCTTGT CAGGCAGTTT 
CAGGGCCATA CGGATGGCGC CAGCTGCATA GATATCTCTC ACGATGGTAC AAAATTATGG 
ACTGGGGGAT TGGATAACAC AGTGCGATGC TGGGACCTGA GGGAGGGCAG ACAGCTCCAA 
CAACATGACT TCAATTCACA GATCTTTTCT CTGGGGTATT GCCCCACTGG GGAATGGCTG 
GCTGTGGGCA TGGAGAGCAG CAATGTTGAG GTTCTGCACG TTTCCAAACC TGATAAGTAC 
CAACTGCATC TTCACGAGAG CTGTGTCCTG TCCCTGCAAT TTGCTTCTTG TGGGAAATGG 
TTTGTGAGTA CTGGGAAGGA CAATTTGCTA AATGCTTGGA GGACGCCATA TGGGGCCAGC 
ATATTCCAGT CAAAAGAATC CTCTTCTGTT CTGAGCTGTG ACGTTTCCAC TGATGACCAA 
TTCATTGTCA CAGGTTCTGG AGACAAAAAG GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961716.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817170.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X1, mRNA.

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CloneID	OXa27436
Clone ID Related Accession (Same CDS sequence)	XM_012961716.2 , XM_012961716.3
Accession Version	XM_012961716.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961716.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGGTACGTTT CATTCCCCCT 
CAACTGACTG TGCACCAGCT GCAGGCCCAG CACCTCTCTC ACCATGCACC TCCAATCCCT 
TTAACCCCAC ATCCATCCAG CCTGCAGCCC CCGGGACTTG CTGCCGCAAC CAGTGCCTCA 
AGCCTATTGG CACTGTCTGG TGTTCTTGGC CTTCAGACAC AACTGGCTGT GAAAGAGGGC 
CTTGCGGATG CAGAACACAG AGAGAGAGAC CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT 
GGAGAGCGGG TGCGCACTCT GTCAGATTAC CTAAACAGCA ACAAGAAGCG GAAAACAGAA 
GAAAAGGATT TTGGCACAGA CTATGGTAGT GATGCTGACC GCAGCGAAGA TAATTTGGTG 
GTTGATGAGG ATCCTGCTTC TCCTCATAGT GTCCATTCTT ACTCCTCTCG GGAAAATGGT 
GTGGACAAAC CTAGTCTACA GAGAAAAGAC CCTCCACCTG CAAGCCCAAA CTCCATGACC 
TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC AAAGACATTC CCTCTCAGCA AGTGGAGAAG 
GCAGGGACCC CTGGCTTAAA GTCTAGCACT CCTAATTCAC AGAGCGATAT GAATACACCG 
GGCCCAAGTG GAACCAGTGC TTCACAGTTT AGGTCTATCT CCTCCAAACC AGCCATTGAC 
TCATTAGCTC TTGGACTGCG GACTCCATTA GGGGGGCAGG GAGGTTATCC TGCTGCATTT 
AGCATTGCTC ACCCGTCTGT GAATGCAGAT GGTGCTGGTG CCTATGCTGG GTTGCACCTC 
ATGTCTCCAC AAATTAATGG TGCCACTGCA GCTGGAACCT ATGGACGATC TCCTTTAGTC 
AGCTATGACC CCCATGCTCA CATGAGAGGA TTGGGTGCTG CAATTCCAGG GTCTGCATCA 
GGAAAACCGG CATACTCTTT TCATGTCAGT GCAGATGGAC AAATGCAGCC AGTTCCCTTT 
CCCCCAGATG CTCTTATCGG CTCAGGAATT CCACGGCACG CACGTCAGCT CCACGTACTG 
AACCATGGGG AGGTAGTCTG TGCGGTGACA ATAAGCAACT CAACACGCCA TGTCTATACT 
GGAGGCAAAG GCTGTGTTAA AGTGTGGGAT GTTGGCCAAC CGGGTACAAA GACTCCTGTG 
GCACAGCTTG ATTGCCTGAA CCGTGACAAT TACATTCGCT CCTGTAAACT TCTGCCTGAT 
GGACGCAGCC TCATTGTTGG CGGGGAAGCC AGCACCTTGT CCATCTGGGA CTTGGCATCT 
CCTACCCCTA GAATAAAGGC AGAGCTTACA TCCTCAGCCC CAGCCTGCTA CGCTCTTGCT 
ATTAGCCCTG ATGCCAAAGT TTGCTTCTCT TGCTGCTCTG ATGGAAACAT TGTAGTGTGG 
GACCTTCAAA ACCAGACCCT TGTCAGGCAG TTTCAGGGCC ATACGGATGG CGCCAGCTGC 
ATAGATATCT CTCACGATGG TACAAAATTA TGGACTGGGG GATTGGATAA CACAGTGCGA 
TGCTGGGACC TGAGGGAGGG CAGACAGCTC CAACAACATG ACTTCAATTC ACAGATCTTT 
TCTCTGGGGT ATTGCCCCAC TGGGGAATGG CTGGCTGTGG GCATGGAGAG CAGCAATGTT 
GAGGTTCTGC ACGTTTCCAA ACCTGATAAG TACCAACTGC ATCTTCACGA GAGCTGTGTC 
CTGTCCCTGC AATTTGCTTC TTGTGGGAAA TGGTTTGTGA GTACTGGGAA GGACAATTTG 
CTAAATGCTT GGAGGACGCC ATATGGGGCC AGCATATTCC AGTCAAAAGA ATCCTCTTCT 
GTTCTGAGCT GTGACGTTTC CACTGATGAC CAATTCATTG TCACAGGTTC TGGAGACAAA 
AAGGCGACAG TATATGAGGT TATCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817170.1
CDS	5..2374
Translation	MREQGKGPIAQTETVPLPPSKMFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGVRFIPPQLTVHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961716.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961716.3

ORF Insert Sequence:

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGGTACGTTT CATTCCCCCT 
CAACTGACTG TGCACCAGCT GCAGGCCCAG CACCTCTCTC ACCATGCACC TCCAATCCCT 
TTAACCCCAC ATCCATCCAG CCTGCAGCCC CCGGGACTTG CTGCCGCAAC CAGTGCCTCA 
AGCCTATTGG CACTGTCTGG TGTTCTTGGC CTTCAGACAC AACTGGCTGT GAAAGAGGGC 
CTTGCGGATG CAGAACACAG AGAGAGAGAC CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT 
GGAGAGCGGG TGCGCACTCT GTCAGATTAC CTAAACAGCA ACAAGAAGCG GAAAACAGAA 
GAAAAGGATT TTGGCACAGA CTATGGTAGT GATGCTGACC GCAGCGAAGA TAATTTGGTG 
GTTGATGAGG ATCCTGCTTC TCCTCATAGT GTCCATTCTT ACTCCTCTCG GGAAAATGGT 
GTGGACAAAC CTAGTCTACA GAGAAAAGAC CCTCCACCTG CAAGCCCAAA CTCCATGACC 
TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC AAAGACATTC CCTCTCAGCA AGTGGAGAAG 
GCAGGGACCC CTGGCTTAAA GTCTAGCACT CCTAATTCAC AGAGCGATAT GAATACACCG 
GGCCCAAGTG GAACCAGTGC TTCACAGTTT AGGTCTATCT CCTCCAAACC AGCCATTGAC 
TCATTAGCTC TTGGACTGCG GACTCCATTA GGGGGGCAGG GAGGTTATCC TGCTGCATTT 
AGCATTGCTC ACCCGTCTGT GAATGCAGAT GGTGCTGGTG CCTATGCTGG GTTGCACCTC 
ATGTCTCCAC AAATTAATGG TGCCACTGCA GCTGGAACCT ATGGACGATC TCCTTTAGTC 
AGCTATGACC CCCATGCTCA CATGAGAGGA TTGGGTGCTG CAATTCCAGG GTCTGCATCA 
GGAAAACCGG CATACTCTTT TCATGTCAGT GCAGATGGAC AAATGCAGCC AGTTCCCTTT 
CCCCCAGATG CTCTTATCGG CTCAGGAATT CCACGGCACG CACGTCAGCT CCACGTACTG 
AACCATGGGG AGGTAGTCTG TGCGGTGACA ATAAGCAACT CAACACGCCA TGTCTATACT 
GGAGGCAAAG GCTGTGTTAA AGTGTGGGAT GTTGGCCAAC CGGGTACAAA GACTCCTGTG 
GCACAGCTTG ATTGCCTGAA CCGTGACAAT TACATTCGCT CCTGTAAACT TCTGCCTGAT 
GGACGCAGCC TCATTGTTGG CGGGGAAGCC AGCACCTTGT CCATCTGGGA CTTGGCATCT 
CCTACCCCTA GAATAAAGGC AGAGCTTACA TCCTCAGCCC CAGCCTGCTA CGCTCTTGCT 
ATTAGCCCTG ATGCCAAAGT TTGCTTCTCT TGCTGCTCTG ATGGAAACAT TGTAGTGTGG 
GACCTTCAAA ACCAGACCCT TGTCAGGCAG TTTCAGGGCC ATACGGATGG CGCCAGCTGC 
ATAGATATCT CTCACGATGG TACAAAATTA TGGACTGGGG GATTGGATAA CACAGTGCGA 
TGCTGGGACC TGAGGGAGGG CAGACAGCTC CAACAACATG ACTTCAATTC ACAGATCTTT 
TCTCTGGGGT ATTGCCCCAC TGGGGAATGG CTGGCTGTGG GCATGGAGAG CAGCAATGTT 
GAGGTTCTGC ACGTTTCCAA ACCTGATAAG TACCAACTGC ATCTTCACGA GAGCTGTGTC 
CTGTCCCTGC AATTTGCTTC TTGTGGGAAA TGGTTTGTGA GTACTGGGAA GGACAATTTG 
CTAAATGCTT GGAGGACGCC ATATGGGGCC AGCATATTCC AGTCAAAAGA ATCCTCTTCT 
GTTCTGAGCT GTGACGTTTC CACTGATGAC CAATTCATTG TCACAGGTTC TGGAGACAAA 
AAGGCGACAG TATATGAGGT TATCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961720.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817174.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X2, mRNA.

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CloneID	OXa27437
Clone ID Related Accession (Same CDS sequence)	XM_012961720.3 , XM_012961720.2
Accession Version	XM_012961720.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGGTACGTTT CATTCCCCCT 
CAACTGACTG TGCACCAGCT GCAGGCCCAG CACCTCTCTC ACCATGCACC TCCAATCCCT 
TTAACCCCAC ATCCATCCAG CCTGCAGCCC CCGGGACTTG CTGCCGCAAC CAGTGCCTCA 
AGCCTATTGG CACTGTCTGG TGTTCTTGGC CTTCAGACAC AACTGGCTGT GAAAGAGGGC 
CTTGCGGATG CAGAACACAG AGAGAGAGAC CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT 
GGAGAGCGGG TGCGCACTCT GTCAGATTAC CTAAACAGCA ACAAGAAGCG GAAAACAGAA 
GAAAAGGATT TTGGCACAGA CTATGGTAGT GATGCTGACC GCAGCGAAGA TAATTTGGTG 
GTTGATGAGG ATCCTGCTTC TCCTCATAGT GTCCATTCTT ACTCCTCTCG GGAAAATGGT 
GTGGACAAAC CTAGTCTACA GAGAAAAGAC CCTCCACCTG CAAGCCCAAA CTCCATGACC 
TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC AAAGACATTC CCTCTCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817174.1
CDS	6..2372
Translation	MREQGKGPIAQTETVPLPPSKMFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGVRFIPPQLTVHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961720.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961720.3

ORF Insert Sequence:

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGGTACGTTT CATTCCCCCT 
CAACTGACTG TGCACCAGCT GCAGGCCCAG CACCTCTCTC ACCATGCACC TCCAATCCCT 
TTAACCCCAC ATCCATCCAG CCTGCAGCCC CCGGGACTTG CTGCCGCAAC CAGTGCCTCA 
AGCCTATTGG CACTGTCTGG TGTTCTTGGC CTTCAGACAC AACTGGCTGT GAAAGAGGGC 
CTTGCGGATG CAGAACACAG AGAGAGAGAC CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT 
GGAGAGCGGG TGCGCACTCT GTCAGATTAC CTAAACAGCA ACAAGAAGCG GAAAACAGAA 
GAAAAGGATT TTGGCACAGA CTATGGTAGT GATGCTGACC GCAGCGAAGA TAATTTGGTG 
GTTGATGAGG ATCCTGCTTC TCCTCATAGT GTCCATTCTT ACTCCTCTCG GGAAAATGGT 
GTGGACAAAC CTAGTCTACA GAGAAAAGAC CCTCCACCTG CAAGCCCAAA CTCCATGACC 
TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC AAAGACATTC CCTCTCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961727.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817181.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa27438
Clone ID Related Accession (Same CDS sequence)	XM_012961727.2 , XM_012961727.3
Accession Version	XM_012961727.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961727.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGCACCAGCT GCAGGCCCAG 
CACCTCTCTC ACCATGCACC TCCAATCCCT TTAACCCCAC ATCCATCCAG CCTGCAGCCC 
CCGGGACTTG CTGCCGCAAC CAGTGCCTCA AGCCTATTGG CACTGTCTGG TGTTCTTGGC 
CTTCAGACAC AACTGGCTGT GAAAGAGGGC CTTGCGGATG CAGAACACAG AGAGAGAGAC 
CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT GGAGAGCGGG TGCGCACTCT GTCAGATTAC 
CTAAACAGCA ACAAGAAGCG GAAAACAGAA GAAAAGGATT TTGGCACAGA CTATGGTAGT 
GATGCTGACC GCAGCGAAGA TAATTTGGTG GTTGATGAGG ATCCTGCTTC TCCTCATAGT 
GTCCATTCTT ACTCCTCTCG GGAAAATGGT GTGGACAAAC CTAGTCTACA GAGAAAAGAC 
CCTCCACCTG CAAGCCCAAA CTCCATGACC TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC 
AAAGACATTC CCTCTCAGCA AGTGGAGAAG GCAGGGACCC CTGGCTTAAA GTCTAGCACT 
CCTAATTCAC AGAGCGATAT GAATACACCG GGCCCAAGTG GAACCAGTGC TTCACAGTTT 
AGGTCTATCT CCTCCAAACC AGCCATTGAC TCATTAGCTC TTGGACTGCG GACTCCATTA 
GGGGGGCAGG GAGGTTATCC TGCTGCATTT AGCATTGCTC ACCCGTCTGT GAATGCAGAT 
GGTGCTGGTG CCTATGCTGG GTTGCACCTC ATGTCTCCAC AAATTAATGG TGCCACTGCA 
GCTGGAACCT ATGGACGATC TCCTTTAGTC AGCTATGACC CCCATGCTCA CATGAGAGGA 
TTGGGTGCTG CAATTCCAGG GTCTGCATCA GGAAAACCGG CATACTCTTT TCATGTCAGT 
GCAGATGGAC AAATGCAGCC AGTTCCCTTT CCCCCAGATG CTCTTATCGG CTCAGGAATT 
CCACGGCACG CACGTCAGCT CCACGTACTG AACCATGGGG AGGTAGTCTG TGCGGTGACA 
ATAAGCAACT CAACACGCCA TGTCTATACT GGAGGCAAAG GCTGTGTTAA AGTGTGGGAT 
GTTGGCCAAC CGGGTACAAA GACTCCTGTG GCACAGCTTG ATTGCCTGAA CCGTGACAAT 
TACATTCGCT CCTGTAAACT TCTGCCTGAT GGACGCAGCC TCATTGTTGG CGGGGAAGCC 
AGCACCTTGT CCATCTGGGA CTTGGCATCT CCTACCCCTA GAATAAAGGC AGAGCTTACA 
TCCTCAGCCC CAGCCTGCTA CGCTCTTGCT ATTAGCCCTG ATGCCAAAGT TTGCTTCTCT 
TGCTGCTCTG ATGGAAACAT TGTAGTGTGG GACCTTCAAA ACCAGACCCT TGTCAGGCAG 
TTTCAGGGCC ATACGGATGG CGCCAGCTGC ATAGATATCT CTCACGATGG TACAAAATTA 
TGGACTGGGG GATTGGATAA CACAGTGCGA TGCTGGGACC TGAGGGAGGG CAGACAGCTC 
CAACAACATG ACTTCAATTC ACAGATCTTT TCTCTGGGGT ATTGCCCCAC TGGGGAATGG 
CTGGCTGTGG GCATGGAGAG CAGCAATGTT GAGGTTCTGC ACGTTTCCAA ACCTGATAAG 
TACCAACTGC ATCTTCACGA GAGCTGTGTC CTGTCCCTGC AATTTGCTTC TTGTGGGAAA 
TGGTTTGTGA GTACTGGGAA GGACAATTTG CTAAATGCTT GGAGGACGCC ATATGGGGCC 
AGCATATTCC AGTCAAAAGA ATCCTCTTCT GTTCTGAGCT GTGACGTTTC CACTGATGAC 
CAATTCATTG TCACAGGTTC TGGAGACAAA AAGGCGACAG TATATGAGGT TATCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817181.1
CDS	6..2345
Translation	MREQGKGPIAQTETVPLPPSKMFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961727.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961727.3

ORF Insert Sequence:

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGCACCAGCT GCAGGCCCAG 
CACCTCTCTC ACCATGCACC TCCAATCCCT TTAACCCCAC ATCCATCCAG CCTGCAGCCC 
CCGGGACTTG CTGCCGCAAC CAGTGCCTCA AGCCTATTGG CACTGTCTGG TGTTCTTGGC 
CTTCAGACAC AACTGGCTGT GAAAGAGGGC CTTGCGGATG CAGAACACAG AGAGAGAGAC 
CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT GGAGAGCGGG TGCGCACTCT GTCAGATTAC 
CTAAACAGCA ACAAGAAGCG GAAAACAGAA GAAAAGGATT TTGGCACAGA CTATGGTAGT 
GATGCTGACC GCAGCGAAGA TAATTTGGTG GTTGATGAGG ATCCTGCTTC TCCTCATAGT 
GTCCATTCTT ACTCCTCTCG GGAAAATGGT GTGGACAAAC CTAGTCTACA GAGAAAAGAC 
CCTCCACCTG CAAGCCCAAA CTCCATGACC TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC 
AAAGACATTC CCTCTCAGCA AGTGGAGAAG GCAGGGACCC CTGGCTTAAA GTCTAGCACT 
CCTAATTCAC AGAGCGATAT GAATACACCG GGCCCAAGTG GAACCAGTGC TTCACAGTTT 
AGGTCTATCT CCTCCAAACC AGCCATTGAC TCATTAGCTC TTGGACTGCG GACTCCATTA 
GGGGGGCAGG GAGGTTATCC TGCTGCATTT AGCATTGCTC ACCCGTCTGT GAATGCAGAT 
GGTGCTGGTG CCTATGCTGG GTTGCACCTC ATGTCTCCAC AAATTAATGG TGCCACTGCA 
GCTGGAACCT ATGGACGATC TCCTTTAGTC AGCTATGACC CCCATGCTCA CATGAGAGGA 
TTGGGTGCTG CAATTCCAGG GTCTGCATCA GGAAAACCGG CATACTCTTT TCATGTCAGT 
GCAGATGGAC AAATGCAGCC AGTTCCCTTT CCCCCAGATG CTCTTATCGG CTCAGGAATT 
CCACGGCACG CACGTCAGCT CCACGTACTG AACCATGGGG AGGTAGTCTG TGCGGTGACA 
ATAAGCAACT CAACACGCCA TGTCTATACT GGAGGCAAAG GCTGTGTTAA AGTGTGGGAT 
GTTGGCCAAC CGGGTACAAA GACTCCTGTG GCACAGCTTG ATTGCCTGAA CCGTGACAAT 
TACATTCGCT CCTGTAAACT TCTGCCTGAT GGACGCAGCC TCATTGTTGG CGGGGAAGCC 
AGCACCTTGT CCATCTGGGA CTTGGCATCT CCTACCCCTA GAATAAAGGC AGAGCTTACA 
TCCTCAGCCC CAGCCTGCTA CGCTCTTGCT ATTAGCCCTG ATGCCAAAGT TTGCTTCTCT 
TGCTGCTCTG ATGGAAACAT TGTAGTGTGG GACCTTCAAA ACCAGACCCT TGTCAGGCAG 
TTTCAGGGCC ATACGGATGG CGCCAGCTGC ATAGATATCT CTCACGATGG TACAAAATTA 
TGGACTGGGG GATTGGATAA CACAGTGCGA TGCTGGGACC TGAGGGAGGG CAGACAGCTC 
CAACAACATG ACTTCAATTC ACAGATCTTT TCTCTGGGGT ATTGCCCCAC TGGGGAATGG 
CTGGCTGTGG GCATGGAGAG CAGCAATGTT GAGGTTCTGC ACGTTTCCAA ACCTGATAAG 
TACCAACTGC ATCTTCACGA GAGCTGTGTC CTGTCCCTGC AATTTGCTTC TTGTGGGAAA 
TGGTTTGTGA GTACTGGGAA GGACAATTTG CTAAATGCTT GGAGGACGCC ATATGGGGCC 
AGCATATTCC AGTCAAAAGA ATCCTCTTCT GTTCTGAGCT GTGACGTTTC CACTGATGAC 
CAATTCATTG TCACAGGTTC TGGAGACAAA AAGGCGACAG TATATGAGGT TATCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961733.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817187.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa27439
Clone ID Related Accession (Same CDS sequence)	XM_012961733.3 , XM_012961733.2
Accession Version	XM_012961733.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961733.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGCACCAGCT GCAGGCCCAG 
CACCTCTCTC ACCATGCACC TCCAATCCCT TTAACCCCAC ATCCATCCAG CCTGCAGCCC 
CCGGGACTTG CTGCCGCAAC CAGTGCCTCA AGCCTATTGG CACTGTCTGG TGTTCTTGGC 
CTTCAGACAC AACTGGCTGT GAAAGAGGGC CTTGCGGATG CAGAACACAG AGAGAGAGAC 
CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT GGAGAGCGGG TGCGCACTCT GTCAGATTAC 
CTAAACAGCA ACAAGAAGCG GAAAACAGAA GAAAAGGATT TTGGCACAGA CTATGGTAGT 
GATGCTGACC GCAGCGAAGA TAATTTGGTG GTTGATGAGG ATCCTGCTTC TCCTCATAGT 
GTCCATTCTT ACTCCTCTCG GGAAAATGGT GTGGACAAAC CTAGTCTACA GAGAAAAGAC 
CCTCCACCTG CAAGCCCAAA CTCCATGACC TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC 
AAAGACATTC CCTCTCAAGT GGAGAAGGCA GGGACCCCTG GCTTAAAGTC TAGCACTCCT 
AATTCACAGA GCGATATGAA TACACCGGGC CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG 
TCTATCTCCT CCAAACCAGC CATTGACTCA TTAGCTCTTG GACTGCGGAC TCCATTAGGG 
GGGCAGGGAG GTTATCCTGC TGCATTTAGC ATTGCTCACC CGTCTGTGAA TGCAGATGGT 
GCTGGTGCCT ATGCTGGGTT GCACCTCATG TCTCCACAAA TTAATGGTGC CACTGCAGCT 
GGAACCTATG GACGATCTCC TTTAGTCAGC TATGACCCCC ATGCTCACAT GAGAGGATTG 
GGTGCTGCAA TTCCAGGGTC TGCATCAGGA AAACCGGCAT ACTCTTTTCA TGTCAGTGCA 
GATGGACAAA TGCAGCCAGT TCCCTTTCCC CCAGATGCTC TTATCGGCTC AGGAATTCCA 
CGGCACGCAC GTCAGCTCCA CGTACTGAAC CATGGGGAGG TAGTCTGTGC GGTGACAATA 
AGCAACTCAA CACGCCATGT CTATACTGGA GGCAAAGGCT GTGTTAAAGT GTGGGATGTT 
GGCCAACCGG GTACAAAGAC TCCTGTGGCA CAGCTTGATT GCCTGAACCG TGACAATTAC 
ATTCGCTCCT GTAAACTTCT GCCTGATGGA CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC 
ACCTTGTCCA TCTGGGACTT GGCATCTCCT ACCCCTAGAA TAAAGGCAGA GCTTACATCC 
TCAGCCCCAG CCTGCTACGC TCTTGCTATT AGCCCTGATG CCAAAGTTTG CTTCTCTTGC 
TGCTCTGATG GAAACATTGT AGTGTGGGAC CTTCAAAACC AGACCCTTGT CAGGCAGTTT 
CAGGGCCATA CGGATGGCGC CAGCTGCATA GATATCTCTC ACGATGGTAC AAAATTATGG 
ACTGGGGGAT TGGATAACAC AGTGCGATGC TGGGACCTGA GGGAGGGCAG ACAGCTCCAA 
CAACATGACT TCAATTCACA GATCTTTTCT CTGGGGTATT GCCCCACTGG GGAATGGCTG 
GCTGTGGGCA TGGAGAGCAG CAATGTTGAG GTTCTGCACG TTTCCAAACC TGATAAGTAC 
CAACTGCATC TTCACGAGAG CTGTGTCCTG TCCCTGCAAT TTGCTTCTTG TGGGAAATGG 
TTTGTGAGTA CTGGGAAGGA CAATTTGCTA AATGCTTGGA GGACGCCATA TGGGGCCAGC 
ATATTCCAGT CAAAAGAATC CTCTTCTGTT CTGAGCTGTG ACGTTTCCAC TGATGACCAA 
TTCATTGTCA CAGGTTCTGG AGACAAAAAG GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817187.1
CDS	6..2342
Translation	MREQGKGPIAQTETVPLPPSKMFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961733.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961733.3

ORF Insert Sequence:

ATGAGAGAAC AGGGGAAAGG ACCGATTGCA CAAACCGAGA CAGTCCCACT ACCTCCTTCA 
AAGATGTTCC CTCAAAACAG ACCTCCGACT CACCTACAAG CCTCAGTGGC AGCAGGAGCA 
GCATCTGGCC CCCCTCAGAG CCTCAAACTT ACCTACCCTG AGACTTTGGA CCGTATCAAG 
GAGGAATTTC AGTTTCTGCA GAGTCAGTAC CACAGTTTAA AGCTGGAGTG TGAAAAGCTG 
GCAACAGAAA AAACAGAGAT CCAGCGTCAT TATGTCATGT ACTATGAAAT GTCTTATGGG 
CTGAATATAG AAATGCACAA ACAAACAGAA ATTGCCAAGC GGCTGAATGT AATTTGTGCC 
CAGCTGGTAC CTTTCCTTTC TCAGGAGCAC CAACAACAGG TTGTCCAGGC TGTAGAACGA 
GCTAAGCAGG TGACTATGGC AGAGCTGAAT GCAGCCATCG GGCACCAGCT GCAGGCCCAG 
CACCTCTCTC ACCATGCACC TCCAATCCCT TTAACCCCAC ATCCATCCAG CCTGCAGCCC 
CCGGGACTTG CTGCCGCAAC CAGTGCCTCA AGCCTATTGG CACTGTCTGG TGTTCTTGGC 
CTTCAGACAC AACTGGCTGT GAAAGAGGGC CTTGCGGATG CAGAACACAG AGAGAGAGAC 
CCAGGGCCTA GCTGTGTAAC TCTTCCTAAT GGAGAGCGGG TGCGCACTCT GTCAGATTAC 
CTAAACAGCA ACAAGAAGCG GAAAACAGAA GAAAAGGATT TTGGCACAGA CTATGGTAGT 
GATGCTGACC GCAGCGAAGA TAATTTGGTG GTTGATGAGG ATCCTGCTTC TCCTCATAGT 
GTCCATTCTT ACTCCTCTCG GGAAAATGGT GTGGACAAAC CTAGTCTACA GAGAAAAGAC 
CCTCCACCTG CAAGCCCAAA CTCCATGACC TCTTCCAGTA GTGTGTCTCC CTCCAGAAGC 
AAAGACATTC CCTCTCAAGT GGAGAAGGCA GGGACCCCTG GCTTAAAGTC TAGCACTCCT 
AATTCACAGA GCGATATGAA TACACCGGGC CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG 
TCTATCTCCT CCAAACCAGC CATTGACTCA TTAGCTCTTG GACTGCGGAC TCCATTAGGG 
GGGCAGGGAG GTTATCCTGC TGCATTTAGC ATTGCTCACC CGTCTGTGAA TGCAGATGGT 
GCTGGTGCCT ATGCTGGGTT GCACCTCATG TCTCCACAAA TTAATGGTGC CACTGCAGCT 
GGAACCTATG GACGATCTCC TTTAGTCAGC TATGACCCCC ATGCTCACAT GAGAGGATTG 
GGTGCTGCAA TTCCAGGGTC TGCATCAGGA AAACCGGCAT ACTCTTTTCA TGTCAGTGCA 
GATGGACAAA TGCAGCCAGT TCCCTTTCCC CCAGATGCTC TTATCGGCTC AGGAATTCCA 
CGGCACGCAC GTCAGCTCCA CGTACTGAAC CATGGGGAGG TAGTCTGTGC GGTGACAATA 
AGCAACTCAA CACGCCATGT CTATACTGGA GGCAAAGGCT GTGTTAAAGT GTGGGATGTT 
GGCCAACCGG GTACAAAGAC TCCTGTGGCA CAGCTTGATT GCCTGAACCG TGACAATTAC 
ATTCGCTCCT GTAAACTTCT GCCTGATGGA CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC 
ACCTTGTCCA TCTGGGACTT GGCATCTCCT ACCCCTAGAA TAAAGGCAGA GCTTACATCC 
TCAGCCCCAG CCTGCTACGC TCTTGCTATT AGCCCTGATG CCAAAGTTTG CTTCTCTTGC 
TGCTCTGATG GAAACATTGT AGTGTGGGAC CTTCAAAACC AGACCCTTGT CAGGCAGTTT 
CAGGGCCATA CGGATGGCGC CAGCTGCATA GATATCTCTC ACGATGGTAC AAAATTATGG 
ACTGGGGGAT TGGATAACAC AGTGCGATGC TGGGACCTGA GGGAGGGCAG ACAGCTCCAA 
CAACATGACT TCAATTCACA GATCTTTTCT CTGGGGTATT GCCCCACTGG GGAATGGCTG 
GCTGTGGGCA TGGAGAGCAG CAATGTTGAG GTTCTGCACG TTTCCAAACC TGATAAGTAC 
CAACTGCATC TTCACGAGAG CTGTGTCCTG TCCCTGCAAT TTGCTTCTTG TGGGAAATGG 
TTTGTGAGTA CTGGGAAGGA CAATTTGCTA AATGCTTGGA GGACGCCATA TGGGGCCAGC 
ATATTCCAGT CAAAAGAATC CTCTTCTGTT CTGAGCTGTG ACGTTTCCAC TGATGACCAA 
TTCATTGTCA CAGGTTCTGG AGACAAAAAG GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961741.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817195.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X6, mRNA.

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CloneID	OXa27440
Clone ID Related Accession (Same CDS sequence)	XM_012961741.2 , XM_012961741.3 , XM_012961745.2 , XM_012961736.2 , XM_012961736.3
Accession Version	XM_012961741.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961741.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGG TACGTTTCAT TCCCCCTCAA 
CTGACTGTGC ACCAGCTGCA GGCCCAGCAC CTCTCTCACC ATGCACCTCC AATCCCTTTA 
ACCCCACATC CATCCAGCCT GCAGCCCCCG GGACTTGCTG CCGCAACCAG TGCCTCAAGC 
CTATTGGCAC TGTCTGGTGT TCTTGGCCTT CAGACACAAC TGGCTGTGAA AGAGGGCCTT 
GCGGATGCAG AACACAGAGA GAGAGACCCA GGGCCTAGCT GTGTAACTCT TCCTAATGGA 
GAGCGGGTGC GCACTCTGTC AGATTACCTA AACAGCAACA AGAAGCGGAA AACAGAAGAA 
AAGGATTTTG GCACAGACTA TGGTAGTGAT GCTGACCGCA GCGAAGATAA TTTGGTGGTT 
GATGAGGATC CTGCTTCTCC TCATAGTGTC CATTCTTACT CCTCTCGGGA AAATGGTGTG 
GACAAACCTA GTCTACAGAG AAAAGACCCT CCACCTGCAA GCCCAAACTC CATGACCTCT 
TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA GACATTCCCT CTCAGCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817195.1
CDS	118..2424
Translation	MFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGVRFIPPQLTVHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961741.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961741.3

ORF Insert Sequence:

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGG TACGTTTCAT TCCCCCTCAA 
CTGACTGTGC ACCAGCTGCA GGCCCAGCAC CTCTCTCACC ATGCACCTCC AATCCCTTTA 
ACCCCACATC CATCCAGCCT GCAGCCCCCG GGACTTGCTG CCGCAACCAG TGCCTCAAGC 
CTATTGGCAC TGTCTGGTGT TCTTGGCCTT CAGACACAAC TGGCTGTGAA AGAGGGCCTT 
GCGGATGCAG AACACAGAGA GAGAGACCCA GGGCCTAGCT GTGTAACTCT TCCTAATGGA 
GAGCGGGTGC GCACTCTGTC AGATTACCTA AACAGCAACA AGAAGCGGAA AACAGAAGAA 
AAGGATTTTG GCACAGACTA TGGTAGTGAT GCTGACCGCA GCGAAGATAA TTTGGTGGTT 
GATGAGGATC CTGCTTCTCC TCATAGTGTC CATTCTTACT CCTCTCGGGA AAATGGTGTG 
GACAAACCTA GTCTACAGAG AAAAGACCCT CCACCTGCAA GCCCAAACTC CATGACCTCT 
TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA GACATTCCCT CTCAGCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961736.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817190.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa27440
Clone ID Related Accession (Same CDS sequence)	XM_012961741.2 , XM_012961741.3 , XM_012961745.2 , XM_012961736.2 , XM_012961736.3
Accession Version	XM_012961736.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961736.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGG TACGTTTCAT TCCCCCTCAA 
CTGACTGTGC ACCAGCTGCA GGCCCAGCAC CTCTCTCACC ATGCACCTCC AATCCCTTTA 
ACCCCACATC CATCCAGCCT GCAGCCCCCG GGACTTGCTG CCGCAACCAG TGCCTCAAGC 
CTATTGGCAC TGTCTGGTGT TCTTGGCCTT CAGACACAAC TGGCTGTGAA AGAGGGCCTT 
GCGGATGCAG AACACAGAGA GAGAGACCCA GGGCCTAGCT GTGTAACTCT TCCTAATGGA 
GAGCGGGTGC GCACTCTGTC AGATTACCTA AACAGCAACA AGAAGCGGAA AACAGAAGAA 
AAGGATTTTG GCACAGACTA TGGTAGTGAT GCTGACCGCA GCGAAGATAA TTTGGTGGTT 
GATGAGGATC CTGCTTCTCC TCATAGTGTC CATTCTTACT CCTCTCGGGA AAATGGTGTG 
GACAAACCTA GTCTACAGAG AAAAGACCCT CCACCTGCAA GCCCAAACTC CATGACCTCT 
TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA GACATTCCCT CTCAGCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817190.1
CDS	227..2533
Translation	MFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGVRFIPPQLTVHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961736.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961736.3

ORF Insert Sequence:

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGG TACGTTTCAT TCCCCCTCAA 
CTGACTGTGC ACCAGCTGCA GGCCCAGCAC CTCTCTCACC ATGCACCTCC AATCCCTTTA 
ACCCCACATC CATCCAGCCT GCAGCCCCCG GGACTTGCTG CCGCAACCAG TGCCTCAAGC 
CTATTGGCAC TGTCTGGTGT TCTTGGCCTT CAGACACAAC TGGCTGTGAA AGAGGGCCTT 
GCGGATGCAG AACACAGAGA GAGAGACCCA GGGCCTAGCT GTGTAACTCT TCCTAATGGA 
GAGCGGGTGC GCACTCTGTC AGATTACCTA AACAGCAACA AGAAGCGGAA AACAGAAGAA 
AAGGATTTTG GCACAGACTA TGGTAGTGAT GCTGACCGCA GCGAAGATAA TTTGGTGGTT 
GATGAGGATC CTGCTTCTCC TCATAGTGTC CATTCTTACT CCTCTCGGGA AAATGGTGTG 
GACAAACCTA GTCTACAGAG AAAAGACCCT CCACCTGCAA GCCCAAACTC CATGACCTCT 
TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA GACATTCCCT CTCAGCAAGT GGAGAAGGCA 
GGGACCCCTG GCTTAAAGTC TAGCACTCCT AATTCACAGA GCGATATGAA TACACCGGGC 
CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG TCTATCTCCT CCAAACCAGC CATTGACTCA 
TTAGCTCTTG GACTGCGGAC TCCATTAGGG GGGCAGGGAG GTTATCCTGC TGCATTTAGC 
ATTGCTCACC CGTCTGTGAA TGCAGATGGT GCTGGTGCCT ATGCTGGGTT GCACCTCATG 
TCTCCACAAA TTAATGGTGC CACTGCAGCT GGAACCTATG GACGATCTCC TTTAGTCAGC 
TATGACCCCC ATGCTCACAT GAGAGGATTG GGTGCTGCAA TTCCAGGGTC TGCATCAGGA 
AAACCGGCAT ACTCTTTTCA TGTCAGTGCA GATGGACAAA TGCAGCCAGT TCCCTTTCCC 
CCAGATGCTC TTATCGGCTC AGGAATTCCA CGGCACGCAC GTCAGCTCCA CGTACTGAAC 
CATGGGGAGG TAGTCTGTGC GGTGACAATA AGCAACTCAA CACGCCATGT CTATACTGGA 
GGCAAAGGCT GTGTTAAAGT GTGGGATGTT GGCCAACCGG GTACAAAGAC TCCTGTGGCA 
CAGCTTGATT GCCTGAACCG TGACAATTAC ATTCGCTCCT GTAAACTTCT GCCTGATGGA 
CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC ACCTTGTCCA TCTGGGACTT GGCATCTCCT 
ACCCCTAGAA TAAAGGCAGA GCTTACATCC TCAGCCCCAG CCTGCTACGC TCTTGCTATT 
AGCCCTGATG CCAAAGTTTG CTTCTCTTGC TGCTCTGATG GAAACATTGT AGTGTGGGAC 
CTTCAAAACC AGACCCTTGT CAGGCAGTTT CAGGGCCATA CGGATGGCGC CAGCTGCATA 
GATATCTCTC ACGATGGTAC AAAATTATGG ACTGGGGGAT TGGATAACAC AGTGCGATGC 
TGGGACCTGA GGGAGGGCAG ACAGCTCCAA CAACATGACT TCAATTCACA GATCTTTTCT 
CTGGGGTATT GCCCCACTGG GGAATGGCTG GCTGTGGGCA TGGAGAGCAG CAATGTTGAG 
GTTCTGCACG TTTCCAAACC TGATAAGTAC CAACTGCATC TTCACGAGAG CTGTGTCCTG 
TCCCTGCAAT TTGCTTCTTG TGGGAAATGG TTTGTGAGTA CTGGGAAGGA CAATTTGCTA 
AATGCTTGGA GGACGCCATA TGGGGCCAGC ATATTCCAGT CAAAAGAATC CTCTTCTGTT 
CTGAGCTGTG ACGTTTCCAC TGATGACCAA TTCATTGTCA CAGGTTCTGG AGACAAAAAG 
GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tle2 ( XM_012961752.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817206.1 Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X7, mRNA.

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CloneID	OXa27441
Clone ID Related Accession (Same CDS sequence)	XM_012961752.2 , XM_012961756.2 , XM_012961752.3
Accession Version	XM_012961752.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transducin like enhancer of split 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961752.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGC ACCAGCTGCA GGCCCAGCAC 
CTCTCTCACC ATGCACCTCC AATCCCTTTA ACCCCACATC CATCCAGCCT GCAGCCCCCG 
GGACTTGCTG CCGCAACCAG TGCCTCAAGC CTATTGGCAC TGTCTGGTGT TCTTGGCCTT 
CAGACACAAC TGGCTGTGAA AGAGGGCCTT GCGGATGCAG AACACAGAGA GAGAGACCCA 
GGGCCTAGCT GTGTAACTCT TCCTAATGGA GAGCGGGTGC GCACTCTGTC AGATTACCTA 
AACAGCAACA AGAAGCGGAA AACAGAAGAA AAGGATTTTG GCACAGACTA TGGTAGTGAT 
GCTGACCGCA GCGAAGATAA TTTGGTGGTT GATGAGGATC CTGCTTCTCC TCATAGTGTC 
CATTCTTACT CCTCTCGGGA AAATGGTGTG GACAAACCTA GTCTACAGAG AAAAGACCCT 
CCACCTGCAA GCCCAAACTC CATGACCTCT TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA 
GACATTCCCT CTCAGCAAGT GGAGAAGGCA GGGACCCCTG GCTTAAAGTC TAGCACTCCT 
AATTCACAGA GCGATATGAA TACACCGGGC CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG 
TCTATCTCCT CCAAACCAGC CATTGACTCA TTAGCTCTTG GACTGCGGAC TCCATTAGGG 
GGGCAGGGAG GTTATCCTGC TGCATTTAGC ATTGCTCACC CGTCTGTGAA TGCAGATGGT 
GCTGGTGCCT ATGCTGGGTT GCACCTCATG TCTCCACAAA TTAATGGTGC CACTGCAGCT 
GGAACCTATG GACGATCTCC TTTAGTCAGC TATGACCCCC ATGCTCACAT GAGAGGATTG 
GGTGCTGCAA TTCCAGGGTC TGCATCAGGA AAACCGGCAT ACTCTTTTCA TGTCAGTGCA 
GATGGACAAA TGCAGCCAGT TCCCTTTCCC CCAGATGCTC TTATCGGCTC AGGAATTCCA 
CGGCACGCAC GTCAGCTCCA CGTACTGAAC CATGGGGAGG TAGTCTGTGC GGTGACAATA 
AGCAACTCAA CACGCCATGT CTATACTGGA GGCAAAGGCT GTGTTAAAGT GTGGGATGTT 
GGCCAACCGG GTACAAAGAC TCCTGTGGCA CAGCTTGATT GCCTGAACCG TGACAATTAC 
ATTCGCTCCT GTAAACTTCT GCCTGATGGA CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC 
ACCTTGTCCA TCTGGGACTT GGCATCTCCT ACCCCTAGAA TAAAGGCAGA GCTTACATCC 
TCAGCCCCAG CCTGCTACGC TCTTGCTATT AGCCCTGATG CCAAAGTTTG CTTCTCTTGC 
TGCTCTGATG GAAACATTGT AGTGTGGGAC CTTCAAAACC AGACCCTTGT CAGGCAGTTT 
CAGGGCCATA CGGATGGCGC CAGCTGCATA GATATCTCTC ACGATGGTAC AAAATTATGG 
ACTGGGGGAT TGGATAACAC AGTGCGATGC TGGGACCTGA GGGAGGGCAG ACAGCTCCAA 
CAACATGACT TCAATTCACA GATCTTTTCT CTGGGGTATT GCCCCACTGG GGAATGGCTG 
GCTGTGGGCA TGGAGAGCAG CAATGTTGAG GTTCTGCACG TTTCCAAACC TGATAAGTAC 
CAACTGCATC TTCACGAGAG CTGTGTCCTG TCCCTGCAAT TTGCTTCTTG TGGGAAATGG 
TTTGTGAGTA CTGGGAAGGA CAATTTGCTA AATGCTTGGA GGACGCCATA TGGGGCCAGC 
ATATTCCAGT CAAAAGAATC CTCTTCTGTT CTGAGCTGTG ACGTTTCCAC TGATGACCAA 
TTCATTGTCA CAGGTTCTGG AGACAAAAAG GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817206.1
CDS	228..2504
Translation	MFPQNRPPTHLQASVAAGAASGPPQSLKLTYPETLDRIKEEFQFLQSQYHSLKLECEKLATEKTEIQRHYVMYYEMSYGLNIEMHKQTEIAKRLNVICAQLVPFLSQEHQQQVVQAVERAKQVTMAELNAAIGHQLQAQHLSHHAPPIPLTPHPSSLQPPGLAAATSASSLLALSGVLGLQTQLAVKEGLADAEHRERDPGPSCVTLPNGERVRTLSDYLNSNKKRKTEEKDFGTDYGSDADRSEDNLVVDEDPASPHSVHSYSSRENGVDKPSLQRKDPPPASPNSMTSSSSVSPSRSKDIPSQQVEKAGTPGLKSSTPNSQSDMNTPGPSGTSASQFRSISSKPAIDSLALGLRTPLGGQGGYPAAFSIAHPSVNADGAGAYAGLHLMSPQINGATAAGTYGRSPLVSYDPHAHMRGLGAAIPGSASGKPAYSFHVSADGQMQPVPFPPDALIGSGIPRHARQLHVLNHGEVVCAVTISNSTRHVYTGGKGCVKVWDVGQPGTKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIVVWDLQNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRCWDLREGRQLQQHDFNSQIFSLGYCPTGEWLAVGMESSNVEVLHVSKPDKYQLHLHESCVLSLQFASCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDVSTDDQFIVTGSGDKKATVYEVIY

Target ORF information:

RefSeq Version	XM_012961752.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transducin like enhancer of split 2 (tle2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961752.3

ORF Insert Sequence:

ATGTTCCCTC AAAACAGACC TCCGACTCAC CTACAAGCCT CAGTGGCAGC AGGAGCAGCA 
TCTGGCCCCC CTCAGAGCCT CAAACTTACC TACCCTGAGA CTTTGGACCG TATCAAGGAG 
GAATTTCAGT TTCTGCAGAG TCAGTACCAC AGTTTAAAGC TGGAGTGTGA AAAGCTGGCA 
ACAGAAAAAA CAGAGATCCA GCGTCATTAT GTCATGTACT ATGAAATGTC TTATGGGCTG 
AATATAGAAA TGCACAAACA AACAGAAATT GCCAAGCGGC TGAATGTAAT TTGTGCCCAG 
CTGGTACCTT TCCTTTCTCA GGAGCACCAA CAACAGGTTG TCCAGGCTGT AGAACGAGCT 
AAGCAGGTGA CTATGGCAGA GCTGAATGCA GCCATCGGGC ACCAGCTGCA GGCCCAGCAC 
CTCTCTCACC ATGCACCTCC AATCCCTTTA ACCCCACATC CATCCAGCCT GCAGCCCCCG 
GGACTTGCTG CCGCAACCAG TGCCTCAAGC CTATTGGCAC TGTCTGGTGT TCTTGGCCTT 
CAGACACAAC TGGCTGTGAA AGAGGGCCTT GCGGATGCAG AACACAGAGA GAGAGACCCA 
GGGCCTAGCT GTGTAACTCT TCCTAATGGA GAGCGGGTGC GCACTCTGTC AGATTACCTA 
AACAGCAACA AGAAGCGGAA AACAGAAGAA AAGGATTTTG GCACAGACTA TGGTAGTGAT 
GCTGACCGCA GCGAAGATAA TTTGGTGGTT GATGAGGATC CTGCTTCTCC TCATAGTGTC 
CATTCTTACT CCTCTCGGGA AAATGGTGTG GACAAACCTA GTCTACAGAG AAAAGACCCT 
CCACCTGCAA GCCCAAACTC CATGACCTCT TCCAGTAGTG TGTCTCCCTC CAGAAGCAAA 
GACATTCCCT CTCAGCAAGT GGAGAAGGCA GGGACCCCTG GCTTAAAGTC TAGCACTCCT 
AATTCACAGA GCGATATGAA TACACCGGGC CCAAGTGGAA CCAGTGCTTC ACAGTTTAGG 
TCTATCTCCT CCAAACCAGC CATTGACTCA TTAGCTCTTG GACTGCGGAC TCCATTAGGG 
GGGCAGGGAG GTTATCCTGC TGCATTTAGC ATTGCTCACC CGTCTGTGAA TGCAGATGGT 
GCTGGTGCCT ATGCTGGGTT GCACCTCATG TCTCCACAAA TTAATGGTGC CACTGCAGCT 
GGAACCTATG GACGATCTCC TTTAGTCAGC TATGACCCCC ATGCTCACAT GAGAGGATTG 
GGTGCTGCAA TTCCAGGGTC TGCATCAGGA AAACCGGCAT ACTCTTTTCA TGTCAGTGCA 
GATGGACAAA TGCAGCCAGT TCCCTTTCCC CCAGATGCTC TTATCGGCTC AGGAATTCCA 
CGGCACGCAC GTCAGCTCCA CGTACTGAAC CATGGGGAGG TAGTCTGTGC GGTGACAATA 
AGCAACTCAA CACGCCATGT CTATACTGGA GGCAAAGGCT GTGTTAAAGT GTGGGATGTT 
GGCCAACCGG GTACAAAGAC TCCTGTGGCA CAGCTTGATT GCCTGAACCG TGACAATTAC 
ATTCGCTCCT GTAAACTTCT GCCTGATGGA CGCAGCCTCA TTGTTGGCGG GGAAGCCAGC 
ACCTTGTCCA TCTGGGACTT GGCATCTCCT ACCCCTAGAA TAAAGGCAGA GCTTACATCC 
TCAGCCCCAG CCTGCTACGC TCTTGCTATT AGCCCTGATG CCAAAGTTTG CTTCTCTTGC 
TGCTCTGATG GAAACATTGT AGTGTGGGAC CTTCAAAACC AGACCCTTGT CAGGCAGTTT 
CAGGGCCATA CGGATGGCGC CAGCTGCATA GATATCTCTC ACGATGGTAC AAAATTATGG 
ACTGGGGGAT TGGATAACAC AGTGCGATGC TGGGACCTGA GGGAGGGCAG ACAGCTCCAA 
CAACATGACT TCAATTCACA GATCTTTTCT CTGGGGTATT GCCCCACTGG GGAATGGCTG 
GCTGTGGGCA TGGAGAGCAG CAATGTTGAG GTTCTGCACG TTTCCAAACC TGATAAGTAC 
CAACTGCATC TTCACGAGAG CTGTGTCCTG TCCCTGCAAT TTGCTTCTTG TGGGAAATGG 
TTTGTGAGTA CTGGGAAGGA CAATTTGCTA AATGCTTGGA GGACGCCATA TGGGGCCAGC 
ATATTCCAGT CAAAAGAATC CTCTTCTGTT CTGAGCTGTG ACGTTTCCAC TGATGACCAA 
TTCATTGTCA CAGGTTCTGG AGACAAAAAG GCGACAGTAT ATGAGGTTAT CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	tle2
Entrez Gene ID	548559
Full Name	transducin like enhancer of split 2
Synonyms	ESG,ESG1,Xesg1,esg2,esg3,grg2,grg3,hst18976,tle3,tle3a,xgrg3
General protein information	Preferred Names transducin like enhancer of split 2 Names transducin like enhancer of split 2 enhancer of split groucho transducin-like enhancer of split 2 (E(sp1) homolog) transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) transducin-like enhancer of split 3 (E(sp1) homolog) transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Danio rerio (zebrafish)	tle2	NP_001124076.1
Caenorhabditis elegans (roundworm)	unc-37	NP_491932.1
Xenopus tropicalis (tropical clawed frog)	tle2	NP_001015842.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

tle2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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