ptk2b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ptk2b
Entrez Gene ID	496459
Full Name	protein tyrosine kinase 2 beta
Synonyms	cadtk,cakb,fadk2,fak2,pkb,ptk,pyk2,raftk
General protein information	Preferred Names protein tyrosine kinase 2 beta Names protein-tyrosine kinase 2-beta PTK2B protein tyrosine kinase 2 beta
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012964036.3	XP_012819490.1	protein-tyrosine kinase 2-beta isoform X1
XM_012964037.2	XP_012819491.1	protein-tyrosine kinase 2-beta isoform X1
XM_012964038.2	XP_012819492.1	protein-tyrosine kinase 2-beta isoform X2
XM_012964037.3	XP_012819491.1	protein-tyrosine kinase 2-beta isoform X1
XM_012964036.2	XP_012819490.1	protein-tyrosine kinase 2-beta isoform X1
NM_001011049.1	NP_001011049.1	protein-tyrosine kinase 2-beta
XM_031901666.1	XP_031757526.1	protein-tyrosine kinase 2-beta isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PTK2B	NP_775267.1
Gallus gallus (chicken)	PTK2B	XP_004935900.1
Pan troglodytes (chimpanzee)	PTK2B	XP_001163381.1
	PTK2B	XP_001109122.1
Bos taurus (cattle)	PTK2B	NP_001095722.1
Mus musculus (house mouse)	Ptk2b	NP_001155838.1
Canis lupus familiaris (dog)	PTK2B	XP_005635736.1
Rattus norvegicus (Norway rat)	Ptk2b	NP_059014.2
Xenopus tropicalis (tropical clawed frog)	ptk2b	NP_001011049.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ptk2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptk2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03022	NM_001011049.1 Latest version!	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa57996	XM_012964037.3 Latest version!	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa57996	XM_012964036.3 Latest version!	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa57996	XM_031901666.1 Latest version!	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa27044	XM_012964037.2 Latest version!	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa27044	XM_012964036.2 Latest version!	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa27045	XM_012964038.2 Latest version!	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ptk2b ( NM_001011049.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001011049.1 Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), mRNA.

Price & Availability↑

CloneID	OXa03022
Clone ID Related Accession (Same CDS sequence)	NM_001011049.1
Accession Version	NM_001011049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-tyrosine kinase 2-beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC084174.1. ##Evidence-Data-START## Transcript exon combination :: BC084174.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028291, SAMD00028293 [ECO:0000350] ##Evidence-Data-END##

ATGCAACAAT ACGCAACGAA AGTGAGCGAA GGCATGGCCT TGCAGCTGGG ATGCCTGGAA 
CTCAGGAGGT TTTATAAAGA TATGCCCCAG AACGCTCTCG AGAAGAAATC CAACTTCGAA 
TTTTTAGAGA AGGAAGTGGG ACTGGATTTG TTCTTCCCAA AACAGATGCA GGAGAGTTTG 
AAGCCTAAAC AATTCCGCAA GATGATCCAG CAGACATTCC AGCAATACGG ATCCCTCAAG 
GAAGAGGAAT GCATTACGCG CTTTATGAAC ACCCTTTCCA CTTTCACCTC CATCGATCAA 
GAAACCTACA GATGTGAATT AGCGCAAGGA TGGAGCATCA CTGTTGACTT GGTTATAGGC 
CCCAAAGGCA TTCGGCAGCT GACTACCAAG GACTCTAAGC CGACATGCTT TGCCGAATTC 
AAGCAGATAA AGTCCATCAA GTGCTCGTTG CTAGAAGATG GCTGGGCCTT GCTGTTACTG 
GGTATATCGG GCGCCCCACA GCCTTTAGCC ATAAAAACAT CGTCGTTGGC AGTGGCGGAA 
AACATGGCCG ACCTCATCGA CGGTTACTGC CGGCTGCAAA ACGAGTCGAA AGATTCGCTC 
ATTGTCAACC CCAAGAAAGA TAATGAGAAG AGGATCAGCC TGCCCCAGAT CCCAACTGAA 
GAAGTACAAG TGCGGCGATC CAGGCTAGTG GAGGGGAACA GTATGGAGTC AGACATATAC 
GCAGAGATTC CGGAGGTGAC TGAGACAAAG CTTACAAATA ACCGTTTCAC CATCAGCCGA 
AGCGACGTGG CGCTGGGGAG GATCCTGGGA GAAGGCTTTT TTGGGGAGGT CTACGATGGC 
GTCTATGTGA AACCGAAGGG CGACAAGATC AACGTGGCGG TAAAGACCTG TAAGAATGAC 
TGCGCCGCCG ACGTCAAGGA GAAATTCATG AGTGAAGCGT ATCTGATGAG GAACTTGGAC 
CACCCGCATA TAGTAAAACT AATTGGCATC ATTGAAGACG ACCCAATATG GATTGTGATG 
GAGCTGTACC CGTACGGAGA GCTTGGGCAG TACCTAGAGA AAAACAAGAA CTCCCTGCAG 
GTTCTCAGCC TCATCCTCTA CTCACTACAG ATCTGCAAAG CCCTCGCCTA CTTAGAGGGA 
ATAAACTGCG TCCACAGGGA CATTGCTGTA CGGAACATTC TGGTAGCTTC TCCTGAGTGT 
GTGAAACTGG GAGACTTTGG CCTTTCTCGG TACCTAGAGG AGGAAGAGTA TTATAAAGCT 
TCAGTCACCC GGCTGCCAAT CAAGTGGATG GCTCCGGAGT CCATTAATTT CCGGCGGTTC 
ACCTCGGCCA GCGATGTCTG GATGTTTGGA GTCTGTATGT GGGAGATACT CTCCTTTGGG 
AAGCAGCCAT TTTTCTGGCT GGAGAACAAA GACGTGATCA GCGTAATCGA GAAGGGAGAT 
CGCCTGCCCA AACCCGAGAT GTGCCCGCCG ACCCTCTACA CCCTGATGAC GCGCTGCTGG 
ACTTATGACC CTTTCGAGAG GCCGAAATTC ACCGAGCTTG TTTGTAGCCT CAGTGATATT 
TATCAGACAG AAAAAGAGAT CATCAAAGAT AAAGAGAGGA ACAACCGCCA GCGGCCTCCT 
AAAATCATTG AGCCTCCTCC CATGCAAGAA CCTCCCCCAA AGCCTAGCCG GCCCAAATAC 
AAGGCAGCCG CGCCCGGAAA TCTGCTGGCA CCTAACCTAC TGACCCAGGT ACCAGAGAGT 
CTATGTGCCA GCTCTCCTAC TCTCTGTAGC CCAACTGACT ACCAGTCTCC ACTGGATTCC 
ACCCGCAGCC CACCACTGCC GCGCCATAGC ATCTTCAAAC GCCGCAGCAT GAGGGAGGAA 
GACTTTGTAA AACCGAACAG CAAAGAAGAA GCTCAGAAAC TGTGGGAAAA GGAAAGGGAC 
AAAATGCTAC AGGTCTTAAA CCGACAGCAA CAACAGATGG AGGAGGACGG CAAGTGGTTG 
AAGCAGGAGG AGAAGTCACT GGCCAATACT CTCAAGGATT CAAAGCAAAA AACAAACAAC 
AACAAGCCCC CTGATGCACT TCCAGAGAAA GAACTGGCGT ACACTGAATT CACAGTCCCC 
CCACAGAAGC CTCCCAGGTT AGGGGCACAG TCCCTACAAC CCCTACAGCC CACACCTACG 
GCCAACATTG ACCGGACAGA TGACGGTGTG TACCAAAGTG TGATGGAGCT GGTAAAGGCG 
GTTCTGCAAC TGAAGAATGA CATCTACCAG CTCCCTCCGG AGGGATACGT TGCCGTTGTT 
AAGAATGTGG GGCTGACCCT TAGAAAACTC ATTGGAAGCG TCGATGAGAT CCTTCCTTCT 
CTCCCACAGA ATTCCCGCAC CGAGATTGAA GGGACCCAGA AGCTCCTAAG CAAAGACCTG 
GCGGAGTTAA TCAACAAGAT GCGCCTGGCC CAACAGAACG CCGTGACGTC GCTGAGCGAA 
GAGTGCAAGA AGCAGATGCT GACGGCGTCG CACACCATGG CCGTAGACGC CAAAAATTTG 
CTGGATGCCG TGGACCAGGC CAGGGTCAGG GCCAATTTAG CCAAGGTGGC CGCTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011049.1
CDS	488..3067
Translation	MQQYATKVSEGMALQLGCLELRRFYKDMPQNALEKKSNFEFLEKEVGLDLFFPKQMQESLKPKQFRKMIQQTFQQYGSLKEEECITRFMNTLSTFTSIDQETYRCELAQGWSITVDLVIGPKGIRQLTTKDSKPTCFAEFKQIKSIKCSLLEDGWALLLLGISGAPQPLAIKTSSLAVAENMADLIDGYCRLQNESKDSLIVNPKKDNEKRISLPQIPTEEVQVRRSRLVEGNSMESDIYAEIPEVTETKLTNNRFTISRSDVALGRILGEGFFGEVYDGVYVKPKGDKINVAVKTCKNDCAADVKEKFMSEAYLMRNLDHPHIVKLIGIIEDDPIWIVMELYPYGELGQYLEKNKNSLQVLSLILYSLQICKALAYLEGINCVHRDIAVRNILVASPECVKLGDFGLSRYLEEEEYYKASVTRLPIKWMAPESINFRRFTSASDVWMFGVCMWEILSFGKQPFFWLENKDVISVIEKGDRLPKPEMCPPTLYTLMTRCWTYDPFERPKFTELVCSLSDIYQTEKEIIKDKERNNRQRPPKIIEPPPMQEPPPKPSRPKYKAAAPGNLLAPNLLTQVPESLCASSPTLCSPTDYQSPLDSTRSPPLPRHSIFKRRSMREEDFVKPNSKEEAQKLWEKERDKMLQVLNRQQQQMEEDGKWLKQEEKSLANTLKDSKQKTNNNKPPDALPEKELAYTEFTVPPQKPPRLGAQSLQPLQPTPTANIDRTDDGVYQSVMELVKAVLQLKNDIYQLPPEGYVAVVKNVGLTLRKLIGSVDEILPSLPQNSRTEIEGTQKLLSKDLAELINKMRLAQQNAVTSLSEECKKQMLTASHTMAVDAKNLLDAVDQARVRANLAKVAAE

Target ORF information:

RefSeq Version	NM_001011049.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011049.1

ORF Insert Sequence:

ATGCAACAAT ACGCAACGAA AGTGAGCGAA GGCATGGCCT TGCAGCTGGG ATGCCTGGAA 
CTCAGGAGGT TTTATAAAGA TATGCCCCAG AACGCTCTCG AGAAGAAATC CAACTTCGAA 
TTTTTAGAGA AGGAAGTGGG ACTGGATTTG TTCTTCCCAA AACAGATGCA GGAGAGTTTG 
AAGCCTAAAC AATTCCGCAA GATGATCCAG CAGACATTCC AGCAATACGG ATCCCTCAAG 
GAAGAGGAAT GCATTACGCG CTTTATGAAC ACCCTTTCCA CTTTCACCTC CATCGATCAA 
GAAACCTACA GATGTGAATT AGCGCAAGGA TGGAGCATCA CTGTTGACTT GGTTATAGGC 
CCCAAAGGCA TTCGGCAGCT GACTACCAAG GACTCTAAGC CGACATGCTT TGCCGAATTC 
AAGCAGATAA AGTCCATCAA GTGCTCGTTG CTAGAAGATG GCTGGGCCTT GCTGTTACTG 
GGTATATCGG GCGCCCCACA GCCTTTAGCC ATAAAAACAT CGTCGTTGGC AGTGGCGGAA 
AACATGGCCG ACCTCATCGA CGGTTACTGC CGGCTGCAAA ACGAGTCGAA AGATTCGCTC 
ATTGTCAACC CCAAGAAAGA TAATGAGAAG AGGATCAGCC TGCCCCAGAT CCCAACTGAA 
GAAGTACAAG TGCGGCGATC CAGGCTAGTG GAGGGGAACA GTATGGAGTC AGACATATAC 
GCAGAGATTC CGGAGGTGAC TGAGACAAAG CTTACAAATA ACCGTTTCAC CATCAGCCGA 
AGCGACGTGG CGCTGGGGAG GATCCTGGGA GAAGGCTTTT TTGGGGAGGT CTACGATGGC 
GTCTATGTGA AACCGAAGGG CGACAAGATC AACGTGGCGG TAAAGACCTG TAAGAATGAC 
TGCGCCGCCG ACGTCAAGGA GAAATTCATG AGTGAAGCGT ATCTGATGAG GAACTTGGAC 
CACCCGCATA TAGTAAAACT AATTGGCATC ATTGAAGACG ACCCAATATG GATTGTGATG 
GAGCTGTACC CGTACGGAGA GCTTGGGCAG TACCTAGAGA AAAACAAGAA CTCCCTGCAG 
GTTCTCAGCC TCATCCTCTA CTCACTACAG ATCTGCAAAG CCCTCGCCTA CTTAGAGGGA 
ATAAACTGCG TCCACAGGGA CATTGCTGTA CGGAACATTC TGGTAGCTTC TCCTGAGTGT 
GTGAAACTGG GAGACTTTGG CCTTTCTCGG TACCTAGAGG AGGAAGAGTA TTATAAAGCT 
TCAGTCACCC GGCTGCCAAT CAAGTGGATG GCTCCGGAGT CCATTAATTT CCGGCGGTTC 
ACCTCGGCCA GCGATGTCTG GATGTTTGGA GTCTGTATGT GGGAGATACT CTCCTTTGGG 
AAGCAGCCAT TTTTCTGGCT GGAGAACAAA GACGTGATCA GCGTAATCGA GAAGGGAGAT 
CGCCTGCCCA AACCCGAGAT GTGCCCGCCG ACCCTCTACA CCCTGATGAC GCGCTGCTGG 
ACTTATGACC CTTTCGAGAG GCCGAAATTC ACCGAGCTTG TTTGTAGCCT CAGTGATATT 
TATCAGACAG AAAAAGAGAT CATCAAAGAT AAAGAGAGGA ACAACCGCCA GCGGCCTCCT 
AAAATCATTG AGCCTCCTCC CATGCAAGAA CCTCCCCCAA AGCCTAGCCG GCCCAAATAC 
AAGGCAGCCG CGCCCGGAAA TCTGCTGGCA CCTAACCTAC TGACCCAGGT ACCAGAGAGT 
CTATGTGCCA GCTCTCCTAC TCTCTGTAGC CCAACTGACT ACCAGTCTCC ACTGGATTCC 
ACCCGCAGCC CACCACTGCC GCGCCATAGC ATCTTCAAAC GCCGCAGCAT GAGGGAGGAA 
GACTTTGTAA AACCGAACAG CAAAGAAGAA GCTCAGAAAC TGTGGGAAAA GGAAAGGGAC 
AAAATGCTAC AGGTCTTAAA CCGACAGCAA CAACAGATGG AGGAGGACGG CAAGTGGTTG 
AAGCAGGAGG AGAAGTCACT GGCCAATACT CTCAAGGATT CAAAGCAAAA AACAAACAAC 
AACAAGCCCC CTGATGCACT TCCAGAGAAA GAACTGGCGT ACACTGAATT CACAGTCCCC 
CCACAGAAGC CTCCCAGGTT AGGGGCACAG TCCCTACAAC CCCTACAGCC CACACCTACG 
GCCAACATTG ACCGGACAGA TGACGGTGTG TACCAAAGTG TGATGGAGCT GGTAAAGGCG 
GTTCTGCAAC TGAAGAATGA CATCTACCAG CTCCCTCCGG AGGGATACGT TGCCGTTGTT 
AAGAATGTGG GGCTGACCCT TAGAAAACTC ATTGGAAGCG TCGATGAGAT CCTTCCTTCT 
CTCCCACAGA ATTCCCGCAC CGAGATTGAA GGGACCCAGA AGCTCCTAAG CAAAGACCTG 
GCGGAGTTAA TCAACAAGAT GCGCCTGGCC CAACAGAACG CCGTGACGTC GCTGAGCGAA 
GAGTGCAAGA AGCAGATGCT GACGGCGTCG CACACCATGG CCGTAGACGC CAAAAATTTG 
CTGGATGCCG TGGACCAGGC CAGGGTCAGG GCCAATTTAG CCAAGGTGGC CGCTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptk2b ( XM_012964036.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819490.1 Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa57996
Clone ID Related Accession (Same CDS sequence)	XM_012964036.3 , XM_012964037.3 , XM_031901666.1
Accession Version	XM_012964036.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-tyrosine kinase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGG ACCATGCCAA CCGGACCAAG GGGGGCACAT TTAACCGGCC AGATGGCCCG 
GCGGAGGTGC CGGCGGAAGT GGAGATCAGT AAGGAGGAGA TGAGAATCCT GAAAGTGTGC 
TTCCACAGTA ACAGCTCCAA CCTGGTAAAA AATTTCAAGC TGGTGAAATG CAGCATCTAC 
ACGGATATAC AGGAGATTAT CAGCTCAATA CTGCTGAGCG GTCGGATAGG GCCGGACATC 
AAACACGTTG AGTGTTACGG TCTGCGTCTG AAGCATCTGA AATCCGATGA GATCCATTGG 
TTGCACCCTG AGCTAACTGT AGGGGAGGTC CAGGAGAAGT ACGAGTGCCT CCATCTTGAG 
GCTGAGTGGA GGTATGACCT GCGGATCCGT TACCTGCCCG AAGACTTCAA TGAAAGTTTT 
AAAAAGGACA AAACTACCTT GTTATATTTC TATCAGCAGC TCAGAAATGA CTACATGCAA 
CAATACGCAA CGAAAGTGAG CGAAGGCATG GCCTTGCAGC TGGGATGCCT GGAACTCAGG 
AGGTTTTATA AAGATATGCC CCAGAACGCT CTCGAGAAGA AATCCAACTT CGAATTTTTA 
GAGAAGGAAG TGGGACTGGA TTTGTTCTTC CCAAAACAGA TGCAGGAGAG TTTGAAGCCT 
AAACAATTCC GCAAGATGAT CCAGCAGACA TTCCAGCAAT ACGGATCCCT CAAGGAAGAG 
GAATGCATTA CGCGCTTTAT GAACACCCTT TCCACTTTCA CCTCCATCGA TCAAGAAACC 
TACAGATGTG AATTAGCGCA AGGATGGAGC ATCACTGTTG ACTTGGTTAT AGGCCCCAAA 
GGCATTCGGC AGCTGACTAC CAAGGACTCT AAGCCGACAT GCTTTGCCGA ATTCAAGCAG 
ATAAAGTCCA TCAAGTGCTC GTTGCTAGAA GATGGCTGGG CCTTGCTGTT ACTGGGTATA 
TCGGGCGCCC CACAGCCTTT AGCCATAAAA ACATCGTCGT TGGCAGTGGC GGAAAACATG 
GCCGACCTCA TCGACGGTTA CTGCCGGCTG CAAAACGAGT CGAAAGATTC GCTCATTGTC 
AACCCCAAGA AAGATAATGA GAAGAGGATC AGCCTGCCCC AGATCCCAAC TGAAGAAGTA 
CAAGTGCGGC GATCCAGGCT AGTGGAGGGG AACAGTATGG AGTCAGACAT ATACGCAGAG 
ATTCCGGAGG TGACTGAGAC AAAGCTTACA AATAACCGTT TCACCATCAG CCGAAGCGAC 
GTGGCGCTGG GGAGGATCCT GGGAGAAGGC TTTTTTGGGG AGGTCTACGA TGGCGTCTAT 
GTGAAACCGA AGGGTGACAA GATCAACGTG GCGGTAAAGA CCTGTAAGAA TGACTGCGCC 
GCCGACGTCA AGGAGAAATT CATGAGTGAA GCGTATCTGA TGAGGAACTT GGACCACCCG 
CATATAGTAA AACTAATTGG CATCATTGAA GACGACCCAA TATGGATTGT GATGGAGCTG 
TACCCGTACG GAGAGCTTGG GCAGTACCTA GAGAAAAACA AGAACTCCCT GCAGGTTCTC 
AGCCTCATCC TCTACTCACT ACAGATCTGC AAAGCCCTCG CCTACTTAGA GGGAATAAAC 
TGCGTCCACA GGGACATTGC TGTACGGAAC ATTCTGGTAG CTTCTCCTGA GTGTGTGAAA 
CTGGGAGACT TTGGCCTTTC TCGGTACCTA GAGGAGGAAG AGTATTATAA AGCTTCAGTC 
ACCCGGCTGC CAATCAAGTG GATGGCTCCG GAGTCCATTA ATTTCCGGCG GTTCACCTCG 
GCCAGCGATG TCTGGATGTT TGGAGTCTGT ATGTGGGAGA TACTCTCCTT TGGGAAGCAG 
CCATTTTTCT GGCTGGAGAA CAAAGACGTG ATCAGCGTAA TCGAGAAGGG AGATCGCCTG 
CCCAAACCCG AGATGTGCCC GCCGACCCTC TACACCCTGA TGACGCGCTG CTGGACTTAT 
GACCCTTTTG AGAGGCCGAA ATTCACCGAG CTTGTTTGTA GCCTCAGTGA TATTTATCAG 
ACAGAAAAAG AGATCATCAA AGATAAAGAG AGGAACAACC GCCAGCGGCC TCCTAAAATC 
ATTGAGCCTC CTCCCATGCA AGAACCTCCC CCAAAGCCTA GCCGGCCCAA ATACAAGGCA 
GCCGCGCCCG GAAATCTGCT GGCACCTAAC CTACTGACCC AGGTACCAGA GAGTCTATGT 
GCCAGCTCTC CTACTCTCTG TAGCCCAACT GACTACCAGT CTCCACTGGA TTCCACCCGC 
AGCCCACCAC TGCCGCGCCA TAGCATCTTC AAACGCCGCA GCATGAGGGA GGAAGACTTT 
GTAAAACCGA ACAGCAAAGA AGAAGCTCAG AAACTGTGGG AAAAGGAAAG GGACAAAATG 
CTACAGGTCT TAAACCGACA GCAACAACAG ATGGAGGAGG ACGGCAAGTG GTTGAAGCAG 
GAGGAGAAGT CACTGGCCAA TACTCTCAAG GATTCAAAGC AAAAAACAAA CAACAACAAG 
CCCCCTGATG CACTTCCAGA GAAAGAACTG GCGTACACTG AATTCACAGT CCCCCCACAG 
AAGCCTCCCA GGTTAGGGGC ACAGTCCCTA CAACCCCTAC AGCCCACACC TACGGCCAAC 
ATTGACCGGA CAGATGACGG TGTGTACCAG AGTGTGATGG AGCTGGTAAA GGCGGTTCTG 
CAACTGAAGA ATGACATCTA CCAGCTCCCT CCGGAGGGAT ACGTTGCCGT TGTTAAGAAT 
GTGGGGCTGA CCCTTAGAAA ACTCATTGGA AGCGTCGATG AGATCCTTCC TTCTCTCCCA 
CAGAATTCCC GCACTGAGAT TGAAGGGACC CAGAAGCTCC TAAGCAAAGA CCTGGCGGAG 
TTAATCAACA AGATGCGCCT GGCCCAACAG AACGCCGTGA CGTCGCTGAG CGAAGAGTGC 
AAGAAGCAGA TGCTGACGGC GTCGCACACC ATGGCCGTAG ACGCCAAAAA TTTGCTGGAT 
GCCGTGGACC AGGCCAGGGT CAGGGCCAAT TTAGCCAAGG TGGCCGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819490.1
CDS	145..3198
Translation	MSLDHANRTKGGTFNRPDGPAEVPAEVEISKEEMRILKVCFHSNSSNLVKNFKLVKCSIYTDIQEIISSILLSGRIGPDIKHVECYGLRLKHLKSDEIHWLHPELTVGEVQEKYECLHLEAEWRYDLRIRYLPEDFNESFKKDKTTLLYFYQQLRNDYMQQYATKVSEGMALQLGCLELRRFYKDMPQNALEKKSNFEFLEKEVGLDLFFPKQMQESLKPKQFRKMIQQTFQQYGSLKEEECITRFMNTLSTFTSIDQETYRCELAQGWSITVDLVIGPKGIRQLTTKDSKPTCFAEFKQIKSIKCSLLEDGWALLLLGISGAPQPLAIKTSSLAVAENMADLIDGYCRLQNESKDSLIVNPKKDNEKRISLPQIPTEEVQVRRSRLVEGNSMESDIYAEIPEVTETKLTNNRFTISRSDVALGRILGEGFFGEVYDGVYVKPKGDKINVAVKTCKNDCAADVKEKFMSEAYLMRNLDHPHIVKLIGIIEDDPIWIVMELYPYGELGQYLEKNKNSLQVLSLILYSLQICKALAYLEGINCVHRDIAVRNILVASPECVKLGDFGLSRYLEEEEYYKASVTRLPIKWMAPESINFRRFTSASDVWMFGVCMWEILSFGKQPFFWLENKDVISVIEKGDRLPKPEMCPPTLYTLMTRCWTYDPFERPKFTELVCSLSDIYQTEKEIIKDKERNNRQRPPKIIEPPPMQEPPPKPSRPKYKAAAPGNLLAPNLLTQVPESLCASSPTLCSPTDYQSPLDSTRSPPLPRHSIFKRRSMREEDFVKPNSKEEAQKLWEKERDKMLQVLNRQQQQMEEDGKWLKQEEKSLANTLKDSKQKTNNNKPPDALPEKELAYTEFTVPPQKPPRLGAQSLQPLQPTPTANIDRTDDGVYQSVMELVKAVLQLKNDIYQLPPEGYVAVVKNVGLTLRKLIGSVDEILPSLPQNSRTEIEGTQKLLSKDLAELINKMRLAQQNAVTSLSEECKKQMLTASHTMAVDAKNLLDAVDQARVRANLAKVAAE

Target ORF information:

RefSeq Version	XM_012964036.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964036.3

ORF Insert Sequence:

ATGTCGCTGG ACCATGCCAA CCGGACCAAG GGGGGCACAT TTAACCGGCC AGATGGCCCG 
GCGGAGGTGC CGGCGGAAGT GGAGATCAGT AAGGAGGAGA TGAGAATCCT GAAAGTGTGC 
TTCCACAGTA ACAGCTCCAA CCTGGTAAAA AATTTCAAGC TGGTGAAATG CAGCATCTAC 
ACGGATATAC AGGAGATTAT CAGCTCAATA CTGCTGAGCG GTCGGATAGG GCCGGACATC 
AAACACGTTG AGTGTTACGG TCTGCGTCTG AAGCATCTGA AATCCGATGA GATCCATTGG 
TTGCACCCTG AGCTAACTGT AGGGGAGGTC CAGGAGAAGT ACGAGTGCCT CCATCTTGAG 
GCTGAGTGGA GGTATGACCT GCGGATCCGT TACCTGCCCG AAGACTTCAA TGAAAGTTTT 
AAAAAGGACA AAACTACCTT GTTATATTTC TATCAGCAGC TCAGAAATGA CTACATGCAA 
CAATACGCAA CGAAAGTGAG CGAAGGCATG GCCTTGCAGC TGGGATGCCT GGAACTCAGG 
AGGTTTTATA AAGATATGCC CCAGAACGCT CTCGAGAAGA AATCCAACTT CGAATTTTTA 
GAGAAGGAAG TGGGACTGGA TTTGTTCTTC CCAAAACAGA TGCAGGAGAG TTTGAAGCCT 
AAACAATTCC GCAAGATGAT CCAGCAGACA TTCCAGCAAT ACGGATCCCT CAAGGAAGAG 
GAATGCATTA CGCGCTTTAT GAACACCCTT TCCACTTTCA CCTCCATCGA TCAAGAAACC 
TACAGATGTG AATTAGCGCA AGGATGGAGC ATCACTGTTG ACTTGGTTAT AGGCCCCAAA 
GGCATTCGGC AGCTGACTAC CAAGGACTCT AAGCCGACAT GCTTTGCCGA ATTCAAGCAG 
ATAAAGTCCA TCAAGTGCTC GTTGCTAGAA GATGGCTGGG CCTTGCTGTT ACTGGGTATA 
TCGGGCGCCC CACAGCCTTT AGCCATAAAA ACATCGTCGT TGGCAGTGGC GGAAAACATG 
GCCGACCTCA TCGACGGTTA CTGCCGGCTG CAAAACGAGT CGAAAGATTC GCTCATTGTC 
AACCCCAAGA AAGATAATGA GAAGAGGATC AGCCTGCCCC AGATCCCAAC TGAAGAAGTA 
CAAGTGCGGC GATCCAGGCT AGTGGAGGGG AACAGTATGG AGTCAGACAT ATACGCAGAG 
ATTCCGGAGG TGACTGAGAC AAAGCTTACA AATAACCGTT TCACCATCAG CCGAAGCGAC 
GTGGCGCTGG GGAGGATCCT GGGAGAAGGC TTTTTTGGGG AGGTCTACGA TGGCGTCTAT 
GTGAAACCGA AGGGTGACAA GATCAACGTG GCGGTAAAGA CCTGTAAGAA TGACTGCGCC 
GCCGACGTCA AGGAGAAATT CATGAGTGAA GCGTATCTGA TGAGGAACTT GGACCACCCG 
CATATAGTAA AACTAATTGG CATCATTGAA GACGACCCAA TATGGATTGT GATGGAGCTG 
TACCCGTACG GAGAGCTTGG GCAGTACCTA GAGAAAAACA AGAACTCCCT GCAGGTTCTC 
AGCCTCATCC TCTACTCACT ACAGATCTGC AAAGCCCTCG CCTACTTAGA GGGAATAAAC 
TGCGTCCACA GGGACATTGC TGTACGGAAC ATTCTGGTAG CTTCTCCTGA GTGTGTGAAA 
CTGGGAGACT TTGGCCTTTC TCGGTACCTA GAGGAGGAAG AGTATTATAA AGCTTCAGTC 
ACCCGGCTGC CAATCAAGTG GATGGCTCCG GAGTCCATTA ATTTCCGGCG GTTCACCTCG 
GCCAGCGATG TCTGGATGTT TGGAGTCTGT ATGTGGGAGA TACTCTCCTT TGGGAAGCAG 
CCATTTTTCT GGCTGGAGAA CAAAGACGTG ATCAGCGTAA TCGAGAAGGG AGATCGCCTG 
CCCAAACCCG AGATGTGCCC GCCGACCCTC TACACCCTGA TGACGCGCTG CTGGACTTAT 
GACCCTTTTG AGAGGCCGAA ATTCACCGAG CTTGTTTGTA GCCTCAGTGA TATTTATCAG 
ACAGAAAAAG AGATCATCAA AGATAAAGAG AGGAACAACC GCCAGCGGCC TCCTAAAATC 
ATTGAGCCTC CTCCCATGCA AGAACCTCCC CCAAAGCCTA GCCGGCCCAA ATACAAGGCA 
GCCGCGCCCG GAAATCTGCT GGCACCTAAC CTACTGACCC AGGTACCAGA GAGTCTATGT 
GCCAGCTCTC CTACTCTCTG TAGCCCAACT GACTACCAGT CTCCACTGGA TTCCACCCGC 
AGCCCACCAC TGCCGCGCCA TAGCATCTTC AAACGCCGCA GCATGAGGGA GGAAGACTTT 
GTAAAACCGA ACAGCAAAGA AGAAGCTCAG AAACTGTGGG AAAAGGAAAG GGACAAAATG 
CTACAGGTCT TAAACCGACA GCAACAACAG ATGGAGGAGG ACGGCAAGTG GTTGAAGCAG 
GAGGAGAAGT CACTGGCCAA TACTCTCAAG GATTCAAAGC AAAAAACAAA CAACAACAAG 
CCCCCTGATG CACTTCCAGA GAAAGAACTG GCGTACACTG AATTCACAGT CCCCCCACAG 
AAGCCTCCCA GGTTAGGGGC ACAGTCCCTA CAACCCCTAC AGCCCACACC TACGGCCAAC 
ATTGACCGGA CAGATGACGG TGTGTACCAG AGTGTGATGG AGCTGGTAAA GGCGGTTCTG 
CAACTGAAGA ATGACATCTA CCAGCTCCCT CCGGAGGGAT ACGTTGCCGT TGTTAAGAAT 
GTGGGGCTGA CCCTTAGAAA ACTCATTGGA AGCGTCGATG AGATCCTTCC TTCTCTCCCA 
CAGAATTCCC GCACTGAGAT TGAAGGGACC CAGAAGCTCC TAAGCAAAGA CCTGGCGGAG 
TTAATCAACA AGATGCGCCT GGCCCAACAG AACGCCGTGA CGTCGCTGAG CGAAGAGTGC 
AAGAAGCAGA TGCTGACGGC GTCGCACACC ATGGCCGTAG ACGCCAAAAA TTTGCTGGAT 
GCCGTGGACC AGGCCAGGGT CAGGGCCAAT TTAGCCAAGG TGGCCGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptk2b ( XM_012964037.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819491.1 Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa57996
Clone ID Related Accession (Same CDS sequence)	XM_012964036.3 , XM_012964037.3 , XM_031901666.1
Accession Version	XM_012964037.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-tyrosine kinase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012964037.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGG ACCATGCCAA CCGGACCAAG GGGGGCACAT TTAACCGGCC AGATGGCCCG 
GCGGAGGTGC CGGCGGAAGT GGAGATCAGT AAGGAGGAGA TGAGAATCCT GAAAGTGTGC 
TTCCACAGTA ACAGCTCCAA CCTGGTAAAA AATTTCAAGC TGGTGAAATG CAGCATCTAC 
ACGGATATAC AGGAGATTAT CAGCTCAATA CTGCTGAGCG GTCGGATAGG GCCGGACATC 
AAACACGTTG AGTGTTACGG TCTGCGTCTG AAGCATCTGA AATCCGATGA GATCCATTGG 
TTGCACCCTG AGCTAACTGT AGGGGAGGTC CAGGAGAAGT ACGAGTGCCT CCATCTTGAG 
GCTGAGTGGA GGTATGACCT GCGGATCCGT TACCTGCCCG AAGACTTCAA TGAAAGTTTT 
AAAAAGGACA AAACTACCTT GTTATATTTC TATCAGCAGC TCAGAAATGA CTACATGCAA 
CAATACGCAA CGAAAGTGAG CGAAGGCATG GCCTTGCAGC TGGGATGCCT GGAACTCAGG 
AGGTTTTATA AAGATATGCC CCAGAACGCT CTCGAGAAGA AATCCAACTT CGAATTTTTA 
GAGAAGGAAG TGGGACTGGA TTTGTTCTTC CCAAAACAGA TGCAGGAGAG TTTGAAGCCT 
AAACAATTCC GCAAGATGAT CCAGCAGACA TTCCAGCAAT ACGGATCCCT CAAGGAAGAG 
GAATGCATTA CGCGCTTTAT GAACACCCTT TCCACTTTCA CCTCCATCGA TCAAGAAACC 
TACAGATGTG AATTAGCGCA AGGATGGAGC ATCACTGTTG ACTTGGTTAT AGGCCCCAAA 
GGCATTCGGC AGCTGACTAC CAAGGACTCT AAGCCGACAT GCTTTGCCGA ATTCAAGCAG 
ATAAAGTCCA TCAAGTGCTC GTTGCTAGAA GATGGCTGGG CCTTGCTGTT ACTGGGTATA 
TCGGGCGCCC CACAGCCTTT AGCCATAAAA ACATCGTCGT TGGCAGTGGC GGAAAACATG 
GCCGACCTCA TCGACGGTTA CTGCCGGCTG CAAAACGAGT CGAAAGATTC GCTCATTGTC 
AACCCCAAGA AAGATAATGA GAAGAGGATC AGCCTGCCCC AGATCCCAAC TGAAGAAGTA 
CAAGTGCGGC GATCCAGGCT AGTGGAGGGG AACAGTATGG AGTCAGACAT ATACGCAGAG 
ATTCCGGAGG TGACTGAGAC AAAGCTTACA AATAACCGTT TCACCATCAG CCGAAGCGAC 
GTGGCGCTGG GGAGGATCCT GGGAGAAGGC TTTTTTGGGG AGGTCTACGA TGGCGTCTAT 
GTGAAACCGA AGGGTGACAA GATCAACGTG GCGGTAAAGA CCTGTAAGAA TGACTGCGCC 
GCCGACGTCA AGGAGAAATT CATGAGTGAA GCGTATCTGA TGAGGAACTT GGACCACCCG 
CATATAGTAA AACTAATTGG CATCATTGAA GACGACCCAA TATGGATTGT GATGGAGCTG 
TACCCGTACG GAGAGCTTGG GCAGTACCTA GAGAAAAACA AGAACTCCCT GCAGGTTCTC 
AGCCTCATCC TCTACTCACT ACAGATCTGC AAAGCCCTCG CCTACTTAGA GGGAATAAAC 
TGCGTCCACA GGGACATTGC TGTACGGAAC ATTCTGGTAG CTTCTCCTGA GTGTGTGAAA 
CTGGGAGACT TTGGCCTTTC TCGGTACCTA GAGGAGGAAG AGTATTATAA AGCTTCAGTC 
ACCCGGCTGC CAATCAAGTG GATGGCTCCG GAGTCCATTA ATTTCCGGCG GTTCACCTCG 
GCCAGCGATG TCTGGATGTT TGGAGTCTGT ATGTGGGAGA TACTCTCCTT TGGGAAGCAG 
CCATTTTTCT GGCTGGAGAA CAAAGACGTG ATCAGCGTAA TCGAGAAGGG AGATCGCCTG 
CCCAAACCCG AGATGTGCCC GCCGACCCTC TACACCCTGA TGACGCGCTG CTGGACTTAT 
GACCCTTTTG AGAGGCCGAA ATTCACCGAG CTTGTTTGTA GCCTCAGTGA TATTTATCAG 
ACAGAAAAAG AGATCATCAA AGATAAAGAG AGGAACAACC GCCAGCGGCC TCCTAAAATC 
ATTGAGCCTC CTCCCATGCA AGAACCTCCC CCAAAGCCTA GCCGGCCCAA ATACAAGGCA 
GCCGCGCCCG GAAATCTGCT GGCACCTAAC CTACTGACCC AGGTACCAGA GAGTCTATGT 
GCCAGCTCTC CTACTCTCTG TAGCCCAACT GACTACCAGT CTCCACTGGA TTCCACCCGC 
AGCCCACCAC TGCCGCGCCA TAGCATCTTC AAACGCCGCA GCATGAGGGA GGAAGACTTT 
GTAAAACCGA ACAGCAAAGA AGAAGCTCAG AAACTGTGGG AAAAGGAAAG GGACAAAATG 
CTACAGGTCT TAAACCGACA GCAACAACAG ATGGAGGAGG ACGGCAAGTG GTTGAAGCAG 
GAGGAGAAGT CACTGGCCAA TACTCTCAAG GATTCAAAGC AAAAAACAAA CAACAACAAG 
CCCCCTGATG CACTTCCAGA GAAAGAACTG GCGTACACTG AATTCACAGT CCCCCCACAG 
AAGCCTCCCA GGTTAGGGGC ACAGTCCCTA CAACCCCTAC AGCCCACACC TACGGCCAAC 
ATTGACCGGA CAGATGACGG TGTGTACCAG AGTGTGATGG AGCTGGTAAA GGCGGTTCTG 
CAACTGAAGA ATGACATCTA CCAGCTCCCT CCGGAGGGAT ACGTTGCCGT TGTTAAGAAT 
GTGGGGCTGA CCCTTAGAAA ACTCATTGGA AGCGTCGATG AGATCCTTCC TTCTCTCCCA 
CAGAATTCCC GCACTGAGAT TGAAGGGACC CAGAAGCTCC TAAGCAAAGA CCTGGCGGAG 
TTAATCAACA AGATGCGCCT GGCCCAACAG AACGCCGTGA CGTCGCTGAG CGAAGAGTGC 
AAGAAGCAGA TGCTGACGGC GTCGCACACC ATGGCCGTAG ACGCCAAAAA TTTGCTGGAT 
GCCGTGGACC AGGCCAGGGT CAGGGCCAAT TTAGCCAAGG TGGCCGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819491.1
CDS	141..3194
Translation	MSLDHANRTKGGTFNRPDGPAEVPAEVEISKEEMRILKVCFHSNSSNLVKNFKLVKCSIYTDIQEIISSILLSGRIGPDIKHVECYGLRLKHLKSDEIHWLHPELTVGEVQEKYECLHLEAEWRYDLRIRYLPEDFNESFKKDKTTLLYFYQQLRNDYMQQYATKVSEGMALQLGCLELRRFYKDMPQNALEKKSNFEFLEKEVGLDLFFPKQMQESLKPKQFRKMIQQTFQQYGSLKEEECITRFMNTLSTFTSIDQETYRCELAQGWSITVDLVIGPKGIRQLTTKDSKPTCFAEFKQIKSIKCSLLEDGWALLLLGISGAPQPLAIKTSSLAVAENMADLIDGYCRLQNESKDSLIVNPKKDNEKRISLPQIPTEEVQVRRSRLVEGNSMESDIYAEIPEVTETKLTNNRFTISRSDVALGRILGEGFFGEVYDGVYVKPKGDKINVAVKTCKNDCAADVKEKFMSEAYLMRNLDHPHIVKLIGIIEDDPIWIVMELYPYGELGQYLEKNKNSLQVLSLILYSLQICKALAYLEGINCVHRDIAVRNILVASPECVKLGDFGLSRYLEEEEYYKASVTRLPIKWMAPESINFRRFTSASDVWMFGVCMWEILSFGKQPFFWLENKDVISVIEKGDRLPKPEMCPPTLYTLMTRCWTYDPFERPKFTELVCSLSDIYQTEKEIIKDKERNNRQRPPKIIEPPPMQEPPPKPSRPKYKAAAPGNLLAPNLLTQVPESLCASSPTLCSPTDYQSPLDSTRSPPLPRHSIFKRRSMREEDFVKPNSKEEAQKLWEKERDKMLQVLNRQQQQMEEDGKWLKQEEKSLANTLKDSKQKTNNNKPPDALPEKELAYTEFTVPPQKPPRLGAQSLQPLQPTPTANIDRTDDGVYQSVMELVKAVLQLKNDIYQLPPEGYVAVVKNVGLTLRKLIGSVDEILPSLPQNSRTEIEGTQKLLSKDLAELINKMRLAQQNAVTSLSEECKKQMLTASHTMAVDAKNLLDAVDQARVRANLAKVAAE

Target ORF information:

RefSeq Version	XM_012964037.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964037.3

ORF Insert Sequence:

ATGTCGCTGG ACCATGCCAA CCGGACCAAG GGGGGCACAT TTAACCGGCC AGATGGCCCG 
GCGGAGGTGC CGGCGGAAGT GGAGATCAGT AAGGAGGAGA TGAGAATCCT GAAAGTGTGC 
TTCCACAGTA ACAGCTCCAA CCTGGTAAAA AATTTCAAGC TGGTGAAATG CAGCATCTAC 
ACGGATATAC AGGAGATTAT CAGCTCAATA CTGCTGAGCG GTCGGATAGG GCCGGACATC 
AAACACGTTG AGTGTTACGG TCTGCGTCTG AAGCATCTGA AATCCGATGA GATCCATTGG 
TTGCACCCTG AGCTAACTGT AGGGGAGGTC CAGGAGAAGT ACGAGTGCCT CCATCTTGAG 
GCTGAGTGGA GGTATGACCT GCGGATCCGT TACCTGCCCG AAGACTTCAA TGAAAGTTTT 
AAAAAGGACA AAACTACCTT GTTATATTTC TATCAGCAGC TCAGAAATGA CTACATGCAA 
CAATACGCAA CGAAAGTGAG CGAAGGCATG GCCTTGCAGC TGGGATGCCT GGAACTCAGG 
AGGTTTTATA AAGATATGCC CCAGAACGCT CTCGAGAAGA AATCCAACTT CGAATTTTTA 
GAGAAGGAAG TGGGACTGGA TTTGTTCTTC CCAAAACAGA TGCAGGAGAG TTTGAAGCCT 
AAACAATTCC GCAAGATGAT CCAGCAGACA TTCCAGCAAT ACGGATCCCT CAAGGAAGAG 
GAATGCATTA CGCGCTTTAT GAACACCCTT TCCACTTTCA CCTCCATCGA TCAAGAAACC 
TACAGATGTG AATTAGCGCA AGGATGGAGC ATCACTGTTG ACTTGGTTAT AGGCCCCAAA 
GGCATTCGGC AGCTGACTAC CAAGGACTCT AAGCCGACAT GCTTTGCCGA ATTCAAGCAG 
ATAAAGTCCA TCAAGTGCTC GTTGCTAGAA GATGGCTGGG CCTTGCTGTT ACTGGGTATA 
TCGGGCGCCC CACAGCCTTT AGCCATAAAA ACATCGTCGT TGGCAGTGGC GGAAAACATG 
GCCGACCTCA TCGACGGTTA CTGCCGGCTG CAAAACGAGT CGAAAGATTC GCTCATTGTC 
AACCCCAAGA AAGATAATGA GAAGAGGATC AGCCTGCCCC AGATCCCAAC TGAAGAAGTA 
CAAGTGCGGC GATCCAGGCT AGTGGAGGGG AACAGTATGG AGTCAGACAT ATACGCAGAG 
ATTCCGGAGG TGACTGAGAC AAAGCTTACA AATAACCGTT TCACCATCAG CCGAAGCGAC 
GTGGCGCTGG GGAGGATCCT GGGAGAAGGC TTTTTTGGGG AGGTCTACGA TGGCGTCTAT 
GTGAAACCGA AGGGTGACAA GATCAACGTG GCGGTAAAGA CCTGTAAGAA TGACTGCGCC 
GCCGACGTCA AGGAGAAATT CATGAGTGAA GCGTATCTGA TGAGGAACTT GGACCACCCG 
CATATAGTAA AACTAATTGG CATCATTGAA GACGACCCAA TATGGATTGT GATGGAGCTG 
TACCCGTACG GAGAGCTTGG GCAGTACCTA GAGAAAAACA AGAACTCCCT GCAGGTTCTC 
AGCCTCATCC TCTACTCACT ACAGATCTGC AAAGCCCTCG CCTACTTAGA GGGAATAAAC 
TGCGTCCACA GGGACATTGC TGTACGGAAC ATTCTGGTAG CTTCTCCTGA GTGTGTGAAA 
CTGGGAGACT TTGGCCTTTC TCGGTACCTA GAGGAGGAAG AGTATTATAA AGCTTCAGTC 
ACCCGGCTGC CAATCAAGTG GATGGCTCCG GAGTCCATTA ATTTCCGGCG GTTCACCTCG 
GCCAGCGATG TCTGGATGTT TGGAGTCTGT ATGTGGGAGA TACTCTCCTT TGGGAAGCAG 
CCATTTTTCT GGCTGGAGAA CAAAGACGTG ATCAGCGTAA TCGAGAAGGG AGATCGCCTG 
CCCAAACCCG AGATGTGCCC GCCGACCCTC TACACCCTGA TGACGCGCTG CTGGACTTAT 
GACCCTTTTG AGAGGCCGAA ATTCACCGAG CTTGTTTGTA GCCTCAGTGA TATTTATCAG 
ACAGAAAAAG AGATCATCAA AGATAAAGAG AGGAACAACC GCCAGCGGCC TCCTAAAATC 
ATTGAGCCTC CTCCCATGCA AGAACCTCCC CCAAAGCCTA GCCGGCCCAA ATACAAGGCA 
GCCGCGCCCG GAAATCTGCT GGCACCTAAC CTACTGACCC AGGTACCAGA GAGTCTATGT 
GCCAGCTCTC CTACTCTCTG TAGCCCAACT GACTACCAGT CTCCACTGGA TTCCACCCGC 
AGCCCACCAC TGCCGCGCCA TAGCATCTTC AAACGCCGCA GCATGAGGGA GGAAGACTTT 
GTAAAACCGA ACAGCAAAGA AGAAGCTCAG AAACTGTGGG AAAAGGAAAG GGACAAAATG 
CTACAGGTCT TAAACCGACA GCAACAACAG ATGGAGGAGG ACGGCAAGTG GTTGAAGCAG 
GAGGAGAAGT CACTGGCCAA TACTCTCAAG GATTCAAAGC AAAAAACAAA CAACAACAAG 
CCCCCTGATG CACTTCCAGA GAAAGAACTG GCGTACACTG AATTCACAGT CCCCCCACAG 
AAGCCTCCCA GGTTAGGGGC ACAGTCCCTA CAACCCCTAC AGCCCACACC TACGGCCAAC 
ATTGACCGGA CAGATGACGG TGTGTACCAG AGTGTGATGG AGCTGGTAAA GGCGGTTCTG 
CAACTGAAGA ATGACATCTA CCAGCTCCCT CCGGAGGGAT ACGTTGCCGT TGTTAAGAAT 
GTGGGGCTGA CCCTTAGAAA ACTCATTGGA AGCGTCGATG AGATCCTTCC TTCTCTCCCA 
CAGAATTCCC GCACTGAGAT TGAAGGGACC CAGAAGCTCC TAAGCAAAGA CCTGGCGGAG 
TTAATCAACA AGATGCGCCT GGCCCAACAG AACGCCGTGA CGTCGCTGAG CGAAGAGTGC 
AAGAAGCAGA TGCTGACGGC GTCGCACACC ATGGCCGTAG ACGCCAAAAA TTTGCTGGAT 
GCCGTGGACC AGGCCAGGGT CAGGGCCAAT TTAGCCAAGG TGGCCGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptk2b ( XM_031901666.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757526.1 Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa57996
Clone ID Related Accession (Same CDS sequence)	XM_012964036.3 , XM_012964037.3 , XM_031901666.1
Accession Version	XM_031901666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-tyrosine kinase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGG ACCATGCCAA CCGGACCAAG GGGGGCACAT TTAACCGGCC AGATGGCCCG 
GCGGAGGTGC CGGCGGAAGT GGAGATCAGT AAGGAGGAGA TGAGAATCCT GAAAGTGTGC 
TTCCACAGTA ACAGCTCCAA CCTGGTAAAA AATTTCAAGC TGGTGAAATG CAGCATCTAC 
ACGGATATAC AGGAGATTAT CAGCTCAATA CTGCTGAGCG GTCGGATAGG GCCGGACATC 
AAACACGTTG AGTGTTACGG TCTGCGTCTG AAGCATCTGA AATCCGATGA GATCCATTGG 
TTGCACCCTG AGCTAACTGT AGGGGAGGTC CAGGAGAAGT ACGAGTGCCT CCATCTTGAG 
GCTGAGTGGA GGTATGACCT GCGGATCCGT TACCTGCCCG AAGACTTCAA TGAAAGTTTT 
AAAAAGGACA AAACTACCTT GTTATATTTC TATCAGCAGC TCAGAAATGA CTACATGCAA 
CAATACGCAA CGAAAGTGAG CGAAGGCATG GCCTTGCAGC TGGGATGCCT GGAACTCAGG 
AGGTTTTATA AAGATATGCC CCAGAACGCT CTCGAGAAGA AATCCAACTT CGAATTTTTA 
GAGAAGGAAG TGGGACTGGA TTTGTTCTTC CCAAAACAGA TGCAGGAGAG TTTGAAGCCT 
AAACAATTCC GCAAGATGAT CCAGCAGACA TTCCAGCAAT ACGGATCCCT CAAGGAAGAG 
GAATGCATTA CGCGCTTTAT GAACACCCTT TCCACTTTCA CCTCCATCGA TCAAGAAACC 
TACAGATGTG AATTAGCGCA AGGATGGAGC ATCACTGTTG ACTTGGTTAT AGGCCCCAAA 
GGCATTCGGC AGCTGACTAC CAAGGACTCT AAGCCGACAT GCTTTGCCGA ATTCAAGCAG 
ATAAAGTCCA TCAAGTGCTC GTTGCTAGAA GATGGCTGGG CCTTGCTGTT ACTGGGTATA 
TCGGGCGCCC CACAGCCTTT AGCCATAAAA ACATCGTCGT TGGCAGTGGC GGAAAACATG 
GCCGACCTCA TCGACGGTTA CTGCCGGCTG CAAAACGAGT CGAAAGATTC GCTCATTGTC 
AACCCCAAGA AAGATAATGA GAAGAGGATC AGCCTGCCCC AGATCCCAAC TGAAGAAGTA 
CAAGTGCGGC GATCCAGGCT AGTGGAGGGG AACAGTATGG AGTCAGACAT ATACGCAGAG 
ATTCCGGAGG TGACTGAGAC AAAGCTTACA AATAACCGTT TCACCATCAG CCGAAGCGAC 
GTGGCGCTGG GGAGGATCCT GGGAGAAGGC TTTTTTGGGG AGGTCTACGA TGGCGTCTAT 
GTGAAACCGA AGGGTGACAA GATCAACGTG GCGGTAAAGA CCTGTAAGAA TGACTGCGCC 
GCCGACGTCA AGGAGAAATT CATGAGTGAA GCGTATCTGA TGAGGAACTT GGACCACCCG 
CATATAGTAA AACTAATTGG CATCATTGAA GACGACCCAA TATGGATTGT GATGGAGCTG 
TACCCGTACG GAGAGCTTGG GCAGTACCTA GAGAAAAACA AGAACTCCCT GCAGGTTCTC 
AGCCTCATCC TCTACTCACT ACAGATCTGC AAAGCCCTCG CCTACTTAGA GGGAATAAAC 
TGCGTCCACA GGGACATTGC TGTACGGAAC ATTCTGGTAG CTTCTCCTGA GTGTGTGAAA 
CTGGGAGACT TTGGCCTTTC TCGGTACCTA GAGGAGGAAG AGTATTATAA AGCTTCAGTC 
ACCCGGCTGC CAATCAAGTG GATGGCTCCG GAGTCCATTA ATTTCCGGCG GTTCACCTCG 
GCCAGCGATG TCTGGATGTT TGGAGTCTGT ATGTGGGAGA TACTCTCCTT TGGGAAGCAG 
CCATTTTTCT GGCTGGAGAA CAAAGACGTG ATCAGCGTAA TCGAGAAGGG AGATCGCCTG 
CCCAAACCCG AGATGTGCCC GCCGACCCTC TACACCCTGA TGACGCGCTG CTGGACTTAT 
GACCCTTTTG AGAGGCCGAA ATTCACCGAG CTTGTTTGTA GCCTCAGTGA TATTTATCAG 
ACAGAAAAAG AGATCATCAA AGATAAAGAG AGGAACAACC GCCAGCGGCC TCCTAAAATC 
ATTGAGCCTC CTCCCATGCA AGAACCTCCC CCAAAGCCTA GCCGGCCCAA ATACAAGGCA 
GCCGCGCCCG GAAATCTGCT GGCACCTAAC CTACTGACCC AGGTACCAGA GAGTCTATGT 
GCCAGCTCTC CTACTCTCTG TAGCCCAACT GACTACCAGT CTCCACTGGA TTCCACCCGC 
AGCCCACCAC TGCCGCGCCA TAGCATCTTC AAACGCCGCA GCATGAGGGA GGAAGACTTT 
GTAAAACCGA ACAGCAAAGA AGAAGCTCAG AAACTGTGGG AAAAGGAAAG GGACAAAATG 
CTACAGGTCT TAAACCGACA GCAACAACAG ATGGAGGAGG ACGGCAAGTG GTTGAAGCAG 
GAGGAGAAGT CACTGGCCAA TACTCTCAAG GATTCAAAGC AAAAAACAAA CAACAACAAG 
CCCCCTGATG CACTTCCAGA GAAAGAACTG GCGTACACTG AATTCACAGT CCCCCCACAG 
AAGCCTCCCA GGTTAGGGGC ACAGTCCCTA CAACCCCTAC AGCCCACACC TACGGCCAAC 
ATTGACCGGA CAGATGACGG TGTGTACCAG AGTGTGATGG AGCTGGTAAA GGCGGTTCTG 
CAACTGAAGA ATGACATCTA CCAGCTCCCT CCGGAGGGAT ACGTTGCCGT TGTTAAGAAT 
GTGGGGCTGA CCCTTAGAAA ACTCATTGGA AGCGTCGATG AGATCCTTCC TTCTCTCCCA 
CAGAATTCCC GCACTGAGAT TGAAGGGACC CAGAAGCTCC TAAGCAAAGA CCTGGCGGAG 
TTAATCAACA AGATGCGCCT GGCCCAACAG AACGCCGTGA CGTCGCTGAG CGAAGAGTGC 
AAGAAGCAGA TGCTGACGGC GTCGCACACC ATGGCCGTAG ACGCCAAAAA TTTGCTGGAT 
GCCGTGGACC AGGCCAGGGT CAGGGCCAAT TTAGCCAAGG TGGCCGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757526.1
CDS	157..3210
Translation	MSLDHANRTKGGTFNRPDGPAEVPAEVEISKEEMRILKVCFHSNSSNLVKNFKLVKCSIYTDIQEIISSILLSGRIGPDIKHVECYGLRLKHLKSDEIHWLHPELTVGEVQEKYECLHLEAEWRYDLRIRYLPEDFNESFKKDKTTLLYFYQQLRNDYMQQYATKVSEGMALQLGCLELRRFYKDMPQNALEKKSNFEFLEKEVGLDLFFPKQMQESLKPKQFRKMIQQTFQQYGSLKEEECITRFMNTLSTFTSIDQETYRCELAQGWSITVDLVIGPKGIRQLTTKDSKPTCFAEFKQIKSIKCSLLEDGWALLLLGISGAPQPLAIKTSSLAVAENMADLIDGYCRLQNESKDSLIVNPKKDNEKRISLPQIPTEEVQVRRSRLVEGNSMESDIYAEIPEVTETKLTNNRFTISRSDVALGRILGEGFFGEVYDGVYVKPKGDKINVAVKTCKNDCAADVKEKFMSEAYLMRNLDHPHIVKLIGIIEDDPIWIVMELYPYGELGQYLEKNKNSLQVLSLILYSLQICKALAYLEGINCVHRDIAVRNILVASPECVKLGDFGLSRYLEEEEYYKASVTRLPIKWMAPESINFRRFTSASDVWMFGVCMWEILSFGKQPFFWLENKDVISVIEKGDRLPKPEMCPPTLYTLMTRCWTYDPFERPKFTELVCSLSDIYQTEKEIIKDKERNNRQRPPKIIEPPPMQEPPPKPSRPKYKAAAPGNLLAPNLLTQVPESLCASSPTLCSPTDYQSPLDSTRSPPLPRHSIFKRRSMREEDFVKPNSKEEAQKLWEKERDKMLQVLNRQQQQMEEDGKWLKQEEKSLANTLKDSKQKTNNNKPPDALPEKELAYTEFTVPPQKPPRLGAQSLQPLQPTPTANIDRTDDGVYQSVMELVKAVLQLKNDIYQLPPEGYVAVVKNVGLTLRKLIGSVDEILPSLPQNSRTEIEGTQKLLSKDLAELINKMRLAQQNAVTSLSEECKKQMLTASHTMAVDAKNLLDAVDQARVRANLAKVAAE

Target ORF information:

RefSeq Version	XM_031901666.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901666.1

ORF Insert Sequence:

ATGTCGCTGG ACCATGCCAA CCGGACCAAG GGGGGCACAT TTAACCGGCC AGATGGCCCG 
GCGGAGGTGC CGGCGGAAGT GGAGATCAGT AAGGAGGAGA TGAGAATCCT GAAAGTGTGC 
TTCCACAGTA ACAGCTCCAA CCTGGTAAAA AATTTCAAGC TGGTGAAATG CAGCATCTAC 
ACGGATATAC AGGAGATTAT CAGCTCAATA CTGCTGAGCG GTCGGATAGG GCCGGACATC 
AAACACGTTG AGTGTTACGG TCTGCGTCTG AAGCATCTGA AATCCGATGA GATCCATTGG 
TTGCACCCTG AGCTAACTGT AGGGGAGGTC CAGGAGAAGT ACGAGTGCCT CCATCTTGAG 
GCTGAGTGGA GGTATGACCT GCGGATCCGT TACCTGCCCG AAGACTTCAA TGAAAGTTTT 
AAAAAGGACA AAACTACCTT GTTATATTTC TATCAGCAGC TCAGAAATGA CTACATGCAA 
CAATACGCAA CGAAAGTGAG CGAAGGCATG GCCTTGCAGC TGGGATGCCT GGAACTCAGG 
AGGTTTTATA AAGATATGCC CCAGAACGCT CTCGAGAAGA AATCCAACTT CGAATTTTTA 
GAGAAGGAAG TGGGACTGGA TTTGTTCTTC CCAAAACAGA TGCAGGAGAG TTTGAAGCCT 
AAACAATTCC GCAAGATGAT CCAGCAGACA TTCCAGCAAT ACGGATCCCT CAAGGAAGAG 
GAATGCATTA CGCGCTTTAT GAACACCCTT TCCACTTTCA CCTCCATCGA TCAAGAAACC 
TACAGATGTG AATTAGCGCA AGGATGGAGC ATCACTGTTG ACTTGGTTAT AGGCCCCAAA 
GGCATTCGGC AGCTGACTAC CAAGGACTCT AAGCCGACAT GCTTTGCCGA ATTCAAGCAG 
ATAAAGTCCA TCAAGTGCTC GTTGCTAGAA GATGGCTGGG CCTTGCTGTT ACTGGGTATA 
TCGGGCGCCC CACAGCCTTT AGCCATAAAA ACATCGTCGT TGGCAGTGGC GGAAAACATG 
GCCGACCTCA TCGACGGTTA CTGCCGGCTG CAAAACGAGT CGAAAGATTC GCTCATTGTC 
AACCCCAAGA AAGATAATGA GAAGAGGATC AGCCTGCCCC AGATCCCAAC TGAAGAAGTA 
CAAGTGCGGC GATCCAGGCT AGTGGAGGGG AACAGTATGG AGTCAGACAT ATACGCAGAG 
ATTCCGGAGG TGACTGAGAC AAAGCTTACA AATAACCGTT TCACCATCAG CCGAAGCGAC 
GTGGCGCTGG GGAGGATCCT GGGAGAAGGC TTTTTTGGGG AGGTCTACGA TGGCGTCTAT 
GTGAAACCGA AGGGTGACAA GATCAACGTG GCGGTAAAGA CCTGTAAGAA TGACTGCGCC 
GCCGACGTCA AGGAGAAATT CATGAGTGAA GCGTATCTGA TGAGGAACTT GGACCACCCG 
CATATAGTAA AACTAATTGG CATCATTGAA GACGACCCAA TATGGATTGT GATGGAGCTG 
TACCCGTACG GAGAGCTTGG GCAGTACCTA GAGAAAAACA AGAACTCCCT GCAGGTTCTC 
AGCCTCATCC TCTACTCACT ACAGATCTGC AAAGCCCTCG CCTACTTAGA GGGAATAAAC 
TGCGTCCACA GGGACATTGC TGTACGGAAC ATTCTGGTAG CTTCTCCTGA GTGTGTGAAA 
CTGGGAGACT TTGGCCTTTC TCGGTACCTA GAGGAGGAAG AGTATTATAA AGCTTCAGTC 
ACCCGGCTGC CAATCAAGTG GATGGCTCCG GAGTCCATTA ATTTCCGGCG GTTCACCTCG 
GCCAGCGATG TCTGGATGTT TGGAGTCTGT ATGTGGGAGA TACTCTCCTT TGGGAAGCAG 
CCATTTTTCT GGCTGGAGAA CAAAGACGTG ATCAGCGTAA TCGAGAAGGG AGATCGCCTG 
CCCAAACCCG AGATGTGCCC GCCGACCCTC TACACCCTGA TGACGCGCTG CTGGACTTAT 
GACCCTTTTG AGAGGCCGAA ATTCACCGAG CTTGTTTGTA GCCTCAGTGA TATTTATCAG 
ACAGAAAAAG AGATCATCAA AGATAAAGAG AGGAACAACC GCCAGCGGCC TCCTAAAATC 
ATTGAGCCTC CTCCCATGCA AGAACCTCCC CCAAAGCCTA GCCGGCCCAA ATACAAGGCA 
GCCGCGCCCG GAAATCTGCT GGCACCTAAC CTACTGACCC AGGTACCAGA GAGTCTATGT 
GCCAGCTCTC CTACTCTCTG TAGCCCAACT GACTACCAGT CTCCACTGGA TTCCACCCGC 
AGCCCACCAC TGCCGCGCCA TAGCATCTTC AAACGCCGCA GCATGAGGGA GGAAGACTTT 
GTAAAACCGA ACAGCAAAGA AGAAGCTCAG AAACTGTGGG AAAAGGAAAG GGACAAAATG 
CTACAGGTCT TAAACCGACA GCAACAACAG ATGGAGGAGG ACGGCAAGTG GTTGAAGCAG 
GAGGAGAAGT CACTGGCCAA TACTCTCAAG GATTCAAAGC AAAAAACAAA CAACAACAAG 
CCCCCTGATG CACTTCCAGA GAAAGAACTG GCGTACACTG AATTCACAGT CCCCCCACAG 
AAGCCTCCCA GGTTAGGGGC ACAGTCCCTA CAACCCCTAC AGCCCACACC TACGGCCAAC 
ATTGACCGGA CAGATGACGG TGTGTACCAG AGTGTGATGG AGCTGGTAAA GGCGGTTCTG 
CAACTGAAGA ATGACATCTA CCAGCTCCCT CCGGAGGGAT ACGTTGCCGT TGTTAAGAAT 
GTGGGGCTGA CCCTTAGAAA ACTCATTGGA AGCGTCGATG AGATCCTTCC TTCTCTCCCA 
CAGAATTCCC GCACTGAGAT TGAAGGGACC CAGAAGCTCC TAAGCAAAGA CCTGGCGGAG 
TTAATCAACA AGATGCGCCT GGCCCAACAG AACGCCGTGA CGTCGCTGAG CGAAGAGTGC 
AAGAAGCAGA TGCTGACGGC GTCGCACACC ATGGCCGTAG ACGCCAAAAA TTTGCTGGAT 
GCCGTGGACC AGGCCAGGGT CAGGGCCAAT TTAGCCAAGG TGGCCGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptk2b ( XM_012964037.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819491.1 Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa27044
Clone ID Related Accession (Same CDS sequence)	XM_012964037.2 , XM_012964036.2
Accession Version	XM_012964037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-tyrosine kinase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGG ACCATGCCAA CCGGACCAAG GGGGGCACAT TTAACCGGCC AGATGGCCCG 
GCGGAGGTGC CGGCGGAAGT GGAGATCAGT AAGGAGGAGA TGAGAATCCT GAAAGTGTGC 
TTCCACAGTA ACAGCTCCAA CCTGGTAAAA AATTTCAAGC TGGTGAAATG CAGCATCTAC 
ACGGATATAC AGGAGATTAT CAGCTCAATA CTGCTGAGCG GTCGGATAGG GCCGGACATC 
AAACACGTTG AGTGTTACGG TCTGCGTCTG AAGCATCTGA AATCCGATGA GATCCATTGG 
TTGCACCCTG AGCTAACTGT AGGGGAGGTC CAGGAGAAGT ACGAGTGCCT CCATCTTGAG 
GCTGAGTGGA GGTATGACCT GCGGATCCGT TACCTGCCCG AAGACTTCAA TGAAAGTTTT 
AAAAAGGACA AAACTACCTT GTTATATTTC TATCAGCAGC TCAGAAATGA CTACATGCAA 
CAATACGCAA CGAAAGTGAG CGAAGGCATG GCCTTGCAGC TGGGATGCCT GGAACTCAGG 
AGGTTTTATA AAGATATGCC CCAGAACGCT CTCGAGAAGA AATCCAACTT CGAATTTTTA 
GAGAAGGAAG TGGGACTGGA TTTGTTCTTC CCAAAACAGA TGCAGGAGAG TTTGAAGCCT 
AAACAATTCC GCAAGATGAT CCAGCAGACA TTCCAGCAAT ACGGATCCCT CAAGGAAGAG 
GAATGCATTA CGCGCTTTAT GAACACCCTT TCCACTTTCA CCTCCATCGA TCAAGAAACC 
TACAGATGTG AATTAGCGCA AGGATGGAGC ATCACTGTTG ACTTGGTTAT AGGCCCCAAA 
GGCATTCGGC AGCTGACTAC CAAGGACTCT AAGCCGACAT GCTTTGCCGA ATTCAAGCAG 
ATAAAGTCCA TCAAGTGCTC GTTGCTAGAA GATGGCTGGG CCTTGCTGTT ACTGGGTATA 
TCGGGCGCCC CACAGCCTTT AGCCATAAAA ACATCGTCGT TGGCAGTGGC GGAAAACATG 
GCCGACCTCA TCGACGGTTA CTGCCGGCTG CAAAACGAGT CGAAAGATTC GCTCATTGTC 
AACCCCAAGA AAGATAATGA GAAGAGGATC AGCCTGCCCC AGATCCCAAC TGAAGAAGTA 
CAAGTGCGGC GATCCAGGCT AGTGGAGGGG AACAGTATGG AGTCAGACAT ATACGCAGAG 
ATTCCGGAGG TGACTGAGAC AAAGCTTACA AATAACCGTT TCACCATCAG CCGAAGCGAC 
GTGGCGCTGG GGAGGATCCT GGGAGAAGGC TTTTTTGGGG AGGTCTACGA TGGCGTCTAT 
GTGAAACCGA AGGGCGACAA GATCAACGTG GCGGTAAAGA CCTGTAAGAA TGACTGCGCC 
GCCGACGTCA AGGAGAAATT CATGAGTGAA GCGTATCTGA TGAGGAACTT GGACCACCCG 
CATATAGTAA AACTAATTGG CATCATTGAA GACGACCCAA TATGGATTGT GATGGAGCTG 
TACCCGTACG GAGAGCTTGG GCAGTACCTA GAGAAAAACA AGAACTCCCT GCAGGTTCTC 
AGCCTCATCC TCTACTCACT ACAGATCTGC AAAGCCCTCG CCTACTTAGA GGGAATAAAC 
TGCGTCCACA GGGACATTGC TGTACGGAAC ATTCTGGTAG CTTCTCCTGA GTGTGTGAAA 
CTGGGAGACT TTGGCCTTTC TCGGTACCTA GAGGAGGAAG AGTATTATAA AGCTTCAGTC 
ACCCGGCTGC CAATCAAGTG GATGGCTCCG GAGTCCATTA ATTTCCGGCG GTTCACCTCG 
GCCAGCGATG TCTGGATGTT TGGAGTCTGT ATGTGGGAGA TACTCTCCTT TGGGAAGCAG 
CCATTTTTCT GGCTGGAGAA CAAAGACGTG ATCAGCGTAA TCGAGAAGGG AGATCGCCTG 
CCCAAACCCG AGATGTGCCC GCCGACCCTC TACACCCTGA TGACGCGCTG CTGGACTTAT 
GACCCTTTCG AGAGGCCGAA ATTCACCGAG CTTGTTTGTA GCCTCAGTGA TATTTATCAG 
ACAGAAAAAG AGATCATCAA AGATAAAGAG AGGAACAACC GCCAGCGGCC TCCTAAAATC 
ATTGAGCCTC CTCCCATGCA AGAACCTCCC CCAAAGCCTA GCCGGCCCAA ATACAAGGCA 
GCCGCGCCCG GAAATCTGCT GGCACCTAAC CTACTGACCC AGGTACCAGA GAGTCTATGT 
GCCAGCTCTC CTACTCTCTG TAGCCCAACT GACTACCAGT CTCCACTGGA TTCCACCCGC 
AGCCCACCAC TGCCGCGCCA TAGCATCTTC AAACGCCGCA GCATGAGGGA GGAAGACTTT 
GTAAAACCGA ACAGCAAAGA AGAAGCTCAG AAACTGTGGG AAAAGGAAAG GGACAAAATG 
CTACAGGTCT TAAACCGACA GCAACAACAG ATGGAGGAGG ACGGCAAGTG GTTGAAGCAG 
GAGGAGAAGT CACTGGCCAA TACTCTCAAG GATTCAAAGC AAAAAACAAA CAACAACAAG 
CCCCCTGATG CACTTCCAGA GAAAGAACTG GCGTACACTG AATTCACAGT CCCCCCACAG 
AAGCCTCCCA GGTTAGGGGC ACAGTCCCTA CAACCCCTAC AGCCCACACC TACGGCCAAC 
ATTGACCGGA CAGATGACGG TGTGTACCAG AGTGTGATGG AGCTGGTAAA GGCGGTTCTG 
CAACTGAAGA ATGACATCTA CCAGCTCCCT CCGGAGGGAT ACGTTGCCGT TGTTAAGAAT 
GTGGGGCTGA CCCTTAGAAA ACTCATTGGA AGCGTCGATG AGATCCTTCC TTCTCTCCCA 
CAGAATTCCC GCACTGAGAT TGAAGGGACC CAGAAGCTCC TAAGCAAAGA CCTGGCGGAG 
TTAATCAACA AGATGCGCCT GGCCCAACAG AACGCCGTGA CGTCGCTGAG CGAAGAGTGC 
AAGAAGCAGA TGCTGACGGC GTCGCACACC ATGGCCGTAG ACGCCAAAAA TTTGCTGGAT 
GCCGTGGACC AGGCCAGGGT CAGGGCCAAT TTAGCCAAGG TGGCCGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819491.1
CDS	135..3188
Translation	MSLDHANRTKGGTFNRPDGPAEVPAEVEISKEEMRILKVCFHSNSSNLVKNFKLVKCSIYTDIQEIISSILLSGRIGPDIKHVECYGLRLKHLKSDEIHWLHPELTVGEVQEKYECLHLEAEWRYDLRIRYLPEDFNESFKKDKTTLLYFYQQLRNDYMQQYATKVSEGMALQLGCLELRRFYKDMPQNALEKKSNFEFLEKEVGLDLFFPKQMQESLKPKQFRKMIQQTFQQYGSLKEEECITRFMNTLSTFTSIDQETYRCELAQGWSITVDLVIGPKGIRQLTTKDSKPTCFAEFKQIKSIKCSLLEDGWALLLLGISGAPQPLAIKTSSLAVAENMADLIDGYCRLQNESKDSLIVNPKKDNEKRISLPQIPTEEVQVRRSRLVEGNSMESDIYAEIPEVTETKLTNNRFTISRSDVALGRILGEGFFGEVYDGVYVKPKGDKINVAVKTCKNDCAADVKEKFMSEAYLMRNLDHPHIVKLIGIIEDDPIWIVMELYPYGELGQYLEKNKNSLQVLSLILYSLQICKALAYLEGINCVHRDIAVRNILVASPECVKLGDFGLSRYLEEEEYYKASVTRLPIKWMAPESINFRRFTSASDVWMFGVCMWEILSFGKQPFFWLENKDVISVIEKGDRLPKPEMCPPTLYTLMTRCWTYDPFERPKFTELVCSLSDIYQTEKEIIKDKERNNRQRPPKIIEPPPMQEPPPKPSRPKYKAAAPGNLLAPNLLTQVPESLCASSPTLCSPTDYQSPLDSTRSPPLPRHSIFKRRSMREEDFVKPNSKEEAQKLWEKERDKMLQVLNRQQQQMEEDGKWLKQEEKSLANTLKDSKQKTNNNKPPDALPEKELAYTEFTVPPQKPPRLGAQSLQPLQPTPTANIDRTDDGVYQSVMELVKAVLQLKNDIYQLPPEGYVAVVKNVGLTLRKLIGSVDEILPSLPQNSRTEIEGTQKLLSKDLAELINKMRLAQQNAVTSLSEECKKQMLTASHTMAVDAKNLLDAVDQARVRANLAKVAAE

Target ORF information:

RefSeq Version	XM_012964037.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964037.2

ORF Insert Sequence:

ATGTCGCTGG ACCATGCCAA CCGGACCAAG GGGGGCACAT TTAACCGGCC AGATGGCCCG 
GCGGAGGTGC CGGCGGAAGT GGAGATCAGT AAGGAGGAGA TGAGAATCCT GAAAGTGTGC 
TTCCACAGTA ACAGCTCCAA CCTGGTAAAA AATTTCAAGC TGGTGAAATG CAGCATCTAC 
ACGGATATAC AGGAGATTAT CAGCTCAATA CTGCTGAGCG GTCGGATAGG GCCGGACATC 
AAACACGTTG AGTGTTACGG TCTGCGTCTG AAGCATCTGA AATCCGATGA GATCCATTGG 
TTGCACCCTG AGCTAACTGT AGGGGAGGTC CAGGAGAAGT ACGAGTGCCT CCATCTTGAG 
GCTGAGTGGA GGTATGACCT GCGGATCCGT TACCTGCCCG AAGACTTCAA TGAAAGTTTT 
AAAAAGGACA AAACTACCTT GTTATATTTC TATCAGCAGC TCAGAAATGA CTACATGCAA 
CAATACGCAA CGAAAGTGAG CGAAGGCATG GCCTTGCAGC TGGGATGCCT GGAACTCAGG 
AGGTTTTATA AAGATATGCC CCAGAACGCT CTCGAGAAGA AATCCAACTT CGAATTTTTA 
GAGAAGGAAG TGGGACTGGA TTTGTTCTTC CCAAAACAGA TGCAGGAGAG TTTGAAGCCT 
AAACAATTCC GCAAGATGAT CCAGCAGACA TTCCAGCAAT ACGGATCCCT CAAGGAAGAG 
GAATGCATTA CGCGCTTTAT GAACACCCTT TCCACTTTCA CCTCCATCGA TCAAGAAACC 
TACAGATGTG AATTAGCGCA AGGATGGAGC ATCACTGTTG ACTTGGTTAT AGGCCCCAAA 
GGCATTCGGC AGCTGACTAC CAAGGACTCT AAGCCGACAT GCTTTGCCGA ATTCAAGCAG 
ATAAAGTCCA TCAAGTGCTC GTTGCTAGAA GATGGCTGGG CCTTGCTGTT ACTGGGTATA 
TCGGGCGCCC CACAGCCTTT AGCCATAAAA ACATCGTCGT TGGCAGTGGC GGAAAACATG 
GCCGACCTCA TCGACGGTTA CTGCCGGCTG CAAAACGAGT CGAAAGATTC GCTCATTGTC 
AACCCCAAGA AAGATAATGA GAAGAGGATC AGCCTGCCCC AGATCCCAAC TGAAGAAGTA 
CAAGTGCGGC GATCCAGGCT AGTGGAGGGG AACAGTATGG AGTCAGACAT ATACGCAGAG 
ATTCCGGAGG TGACTGAGAC AAAGCTTACA AATAACCGTT TCACCATCAG CCGAAGCGAC 
GTGGCGCTGG GGAGGATCCT GGGAGAAGGC TTTTTTGGGG AGGTCTACGA TGGCGTCTAT 
GTGAAACCGA AGGGCGACAA GATCAACGTG GCGGTAAAGA CCTGTAAGAA TGACTGCGCC 
GCCGACGTCA AGGAGAAATT CATGAGTGAA GCGTATCTGA TGAGGAACTT GGACCACCCG 
CATATAGTAA AACTAATTGG CATCATTGAA GACGACCCAA TATGGATTGT GATGGAGCTG 
TACCCGTACG GAGAGCTTGG GCAGTACCTA GAGAAAAACA AGAACTCCCT GCAGGTTCTC 
AGCCTCATCC TCTACTCACT ACAGATCTGC AAAGCCCTCG CCTACTTAGA GGGAATAAAC 
TGCGTCCACA GGGACATTGC TGTACGGAAC ATTCTGGTAG CTTCTCCTGA GTGTGTGAAA 
CTGGGAGACT TTGGCCTTTC TCGGTACCTA GAGGAGGAAG AGTATTATAA AGCTTCAGTC 
ACCCGGCTGC CAATCAAGTG GATGGCTCCG GAGTCCATTA ATTTCCGGCG GTTCACCTCG 
GCCAGCGATG TCTGGATGTT TGGAGTCTGT ATGTGGGAGA TACTCTCCTT TGGGAAGCAG 
CCATTTTTCT GGCTGGAGAA CAAAGACGTG ATCAGCGTAA TCGAGAAGGG AGATCGCCTG 
CCCAAACCCG AGATGTGCCC GCCGACCCTC TACACCCTGA TGACGCGCTG CTGGACTTAT 
GACCCTTTCG AGAGGCCGAA ATTCACCGAG CTTGTTTGTA GCCTCAGTGA TATTTATCAG 
ACAGAAAAAG AGATCATCAA AGATAAAGAG AGGAACAACC GCCAGCGGCC TCCTAAAATC 
ATTGAGCCTC CTCCCATGCA AGAACCTCCC CCAAAGCCTA GCCGGCCCAA ATACAAGGCA 
GCCGCGCCCG GAAATCTGCT GGCACCTAAC CTACTGACCC AGGTACCAGA GAGTCTATGT 
GCCAGCTCTC CTACTCTCTG TAGCCCAACT GACTACCAGT CTCCACTGGA TTCCACCCGC 
AGCCCACCAC TGCCGCGCCA TAGCATCTTC AAACGCCGCA GCATGAGGGA GGAAGACTTT 
GTAAAACCGA ACAGCAAAGA AGAAGCTCAG AAACTGTGGG AAAAGGAAAG GGACAAAATG 
CTACAGGTCT TAAACCGACA GCAACAACAG ATGGAGGAGG ACGGCAAGTG GTTGAAGCAG 
GAGGAGAAGT CACTGGCCAA TACTCTCAAG GATTCAAAGC AAAAAACAAA CAACAACAAG 
CCCCCTGATG CACTTCCAGA GAAAGAACTG GCGTACACTG AATTCACAGT CCCCCCACAG 
AAGCCTCCCA GGTTAGGGGC ACAGTCCCTA CAACCCCTAC AGCCCACACC TACGGCCAAC 
ATTGACCGGA CAGATGACGG TGTGTACCAG AGTGTGATGG AGCTGGTAAA GGCGGTTCTG 
CAACTGAAGA ATGACATCTA CCAGCTCCCT CCGGAGGGAT ACGTTGCCGT TGTTAAGAAT 
GTGGGGCTGA CCCTTAGAAA ACTCATTGGA AGCGTCGATG AGATCCTTCC TTCTCTCCCA 
CAGAATTCCC GCACTGAGAT TGAAGGGACC CAGAAGCTCC TAAGCAAAGA CCTGGCGGAG 
TTAATCAACA AGATGCGCCT GGCCCAACAG AACGCCGTGA CGTCGCTGAG CGAAGAGTGC 
AAGAAGCAGA TGCTGACGGC GTCGCACACC ATGGCCGTAG ACGCCAAAAA TTTGCTGGAT 
GCCGTGGACC AGGCCAGGGT CAGGGCCAAT TTAGCCAAGG TGGCCGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptk2b ( XM_012964036.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819490.1 Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa27044
Clone ID Related Accession (Same CDS sequence)	XM_012964037.2 , XM_012964036.2
Accession Version	XM_012964036.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-tyrosine kinase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964036.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGG ACCATGCCAA CCGGACCAAG GGGGGCACAT TTAACCGGCC AGATGGCCCG 
GCGGAGGTGC CGGCGGAAGT GGAGATCAGT AAGGAGGAGA TGAGAATCCT GAAAGTGTGC 
TTCCACAGTA ACAGCTCCAA CCTGGTAAAA AATTTCAAGC TGGTGAAATG CAGCATCTAC 
ACGGATATAC AGGAGATTAT CAGCTCAATA CTGCTGAGCG GTCGGATAGG GCCGGACATC 
AAACACGTTG AGTGTTACGG TCTGCGTCTG AAGCATCTGA AATCCGATGA GATCCATTGG 
TTGCACCCTG AGCTAACTGT AGGGGAGGTC CAGGAGAAGT ACGAGTGCCT CCATCTTGAG 
GCTGAGTGGA GGTATGACCT GCGGATCCGT TACCTGCCCG AAGACTTCAA TGAAAGTTTT 
AAAAAGGACA AAACTACCTT GTTATATTTC TATCAGCAGC TCAGAAATGA CTACATGCAA 
CAATACGCAA CGAAAGTGAG CGAAGGCATG GCCTTGCAGC TGGGATGCCT GGAACTCAGG 
AGGTTTTATA AAGATATGCC CCAGAACGCT CTCGAGAAGA AATCCAACTT CGAATTTTTA 
GAGAAGGAAG TGGGACTGGA TTTGTTCTTC CCAAAACAGA TGCAGGAGAG TTTGAAGCCT 
AAACAATTCC GCAAGATGAT CCAGCAGACA TTCCAGCAAT ACGGATCCCT CAAGGAAGAG 
GAATGCATTA CGCGCTTTAT GAACACCCTT TCCACTTTCA CCTCCATCGA TCAAGAAACC 
TACAGATGTG AATTAGCGCA AGGATGGAGC ATCACTGTTG ACTTGGTTAT AGGCCCCAAA 
GGCATTCGGC AGCTGACTAC CAAGGACTCT AAGCCGACAT GCTTTGCCGA ATTCAAGCAG 
ATAAAGTCCA TCAAGTGCTC GTTGCTAGAA GATGGCTGGG CCTTGCTGTT ACTGGGTATA 
TCGGGCGCCC CACAGCCTTT AGCCATAAAA ACATCGTCGT TGGCAGTGGC GGAAAACATG 
GCCGACCTCA TCGACGGTTA CTGCCGGCTG CAAAACGAGT CGAAAGATTC GCTCATTGTC 
AACCCCAAGA AAGATAATGA GAAGAGGATC AGCCTGCCCC AGATCCCAAC TGAAGAAGTA 
CAAGTGCGGC GATCCAGGCT AGTGGAGGGG AACAGTATGG AGTCAGACAT ATACGCAGAG 
ATTCCGGAGG TGACTGAGAC AAAGCTTACA AATAACCGTT TCACCATCAG CCGAAGCGAC 
GTGGCGCTGG GGAGGATCCT GGGAGAAGGC TTTTTTGGGG AGGTCTACGA TGGCGTCTAT 
GTGAAACCGA AGGGCGACAA GATCAACGTG GCGGTAAAGA CCTGTAAGAA TGACTGCGCC 
GCCGACGTCA AGGAGAAATT CATGAGTGAA GCGTATCTGA TGAGGAACTT GGACCACCCG 
CATATAGTAA AACTAATTGG CATCATTGAA GACGACCCAA TATGGATTGT GATGGAGCTG 
TACCCGTACG GAGAGCTTGG GCAGTACCTA GAGAAAAACA AGAACTCCCT GCAGGTTCTC 
AGCCTCATCC TCTACTCACT ACAGATCTGC AAAGCCCTCG CCTACTTAGA GGGAATAAAC 
TGCGTCCACA GGGACATTGC TGTACGGAAC ATTCTGGTAG CTTCTCCTGA GTGTGTGAAA 
CTGGGAGACT TTGGCCTTTC TCGGTACCTA GAGGAGGAAG AGTATTATAA AGCTTCAGTC 
ACCCGGCTGC CAATCAAGTG GATGGCTCCG GAGTCCATTA ATTTCCGGCG GTTCACCTCG 
GCCAGCGATG TCTGGATGTT TGGAGTCTGT ATGTGGGAGA TACTCTCCTT TGGGAAGCAG 
CCATTTTTCT GGCTGGAGAA CAAAGACGTG ATCAGCGTAA TCGAGAAGGG AGATCGCCTG 
CCCAAACCCG AGATGTGCCC GCCGACCCTC TACACCCTGA TGACGCGCTG CTGGACTTAT 
GACCCTTTCG AGAGGCCGAA ATTCACCGAG CTTGTTTGTA GCCTCAGTGA TATTTATCAG 
ACAGAAAAAG AGATCATCAA AGATAAAGAG AGGAACAACC GCCAGCGGCC TCCTAAAATC 
ATTGAGCCTC CTCCCATGCA AGAACCTCCC CCAAAGCCTA GCCGGCCCAA ATACAAGGCA 
GCCGCGCCCG GAAATCTGCT GGCACCTAAC CTACTGACCC AGGTACCAGA GAGTCTATGT 
GCCAGCTCTC CTACTCTCTG TAGCCCAACT GACTACCAGT CTCCACTGGA TTCCACCCGC 
AGCCCACCAC TGCCGCGCCA TAGCATCTTC AAACGCCGCA GCATGAGGGA GGAAGACTTT 
GTAAAACCGA ACAGCAAAGA AGAAGCTCAG AAACTGTGGG AAAAGGAAAG GGACAAAATG 
CTACAGGTCT TAAACCGACA GCAACAACAG ATGGAGGAGG ACGGCAAGTG GTTGAAGCAG 
GAGGAGAAGT CACTGGCCAA TACTCTCAAG GATTCAAAGC AAAAAACAAA CAACAACAAG 
CCCCCTGATG CACTTCCAGA GAAAGAACTG GCGTACACTG AATTCACAGT CCCCCCACAG 
AAGCCTCCCA GGTTAGGGGC ACAGTCCCTA CAACCCCTAC AGCCCACACC TACGGCCAAC 
ATTGACCGGA CAGATGACGG TGTGTACCAG AGTGTGATGG AGCTGGTAAA GGCGGTTCTG 
CAACTGAAGA ATGACATCTA CCAGCTCCCT CCGGAGGGAT ACGTTGCCGT TGTTAAGAAT 
GTGGGGCTGA CCCTTAGAAA ACTCATTGGA AGCGTCGATG AGATCCTTCC TTCTCTCCCA 
CAGAATTCCC GCACTGAGAT TGAAGGGACC CAGAAGCTCC TAAGCAAAGA CCTGGCGGAG 
TTAATCAACA AGATGCGCCT GGCCCAACAG AACGCCGTGA CGTCGCTGAG CGAAGAGTGC 
AAGAAGCAGA TGCTGACGGC GTCGCACACC ATGGCCGTAG ACGCCAAAAA TTTGCTGGAT 
GCCGTGGACC AGGCCAGGGT CAGGGCCAAT TTAGCCAAGG TGGCCGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819490.1
CDS	194..3247
Translation	MSLDHANRTKGGTFNRPDGPAEVPAEVEISKEEMRILKVCFHSNSSNLVKNFKLVKCSIYTDIQEIISSILLSGRIGPDIKHVECYGLRLKHLKSDEIHWLHPELTVGEVQEKYECLHLEAEWRYDLRIRYLPEDFNESFKKDKTTLLYFYQQLRNDYMQQYATKVSEGMALQLGCLELRRFYKDMPQNALEKKSNFEFLEKEVGLDLFFPKQMQESLKPKQFRKMIQQTFQQYGSLKEEECITRFMNTLSTFTSIDQETYRCELAQGWSITVDLVIGPKGIRQLTTKDSKPTCFAEFKQIKSIKCSLLEDGWALLLLGISGAPQPLAIKTSSLAVAENMADLIDGYCRLQNESKDSLIVNPKKDNEKRISLPQIPTEEVQVRRSRLVEGNSMESDIYAEIPEVTETKLTNNRFTISRSDVALGRILGEGFFGEVYDGVYVKPKGDKINVAVKTCKNDCAADVKEKFMSEAYLMRNLDHPHIVKLIGIIEDDPIWIVMELYPYGELGQYLEKNKNSLQVLSLILYSLQICKALAYLEGINCVHRDIAVRNILVASPECVKLGDFGLSRYLEEEEYYKASVTRLPIKWMAPESINFRRFTSASDVWMFGVCMWEILSFGKQPFFWLENKDVISVIEKGDRLPKPEMCPPTLYTLMTRCWTYDPFERPKFTELVCSLSDIYQTEKEIIKDKERNNRQRPPKIIEPPPMQEPPPKPSRPKYKAAAPGNLLAPNLLTQVPESLCASSPTLCSPTDYQSPLDSTRSPPLPRHSIFKRRSMREEDFVKPNSKEEAQKLWEKERDKMLQVLNRQQQQMEEDGKWLKQEEKSLANTLKDSKQKTNNNKPPDALPEKELAYTEFTVPPQKPPRLGAQSLQPLQPTPTANIDRTDDGVYQSVMELVKAVLQLKNDIYQLPPEGYVAVVKNVGLTLRKLIGSVDEILPSLPQNSRTEIEGTQKLLSKDLAELINKMRLAQQNAVTSLSEECKKQMLTASHTMAVDAKNLLDAVDQARVRANLAKVAAE

Target ORF information:

RefSeq Version	XM_012964036.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964036.2

ORF Insert Sequence:

ATGTCGCTGG ACCATGCCAA CCGGACCAAG GGGGGCACAT TTAACCGGCC AGATGGCCCG 
GCGGAGGTGC CGGCGGAAGT GGAGATCAGT AAGGAGGAGA TGAGAATCCT GAAAGTGTGC 
TTCCACAGTA ACAGCTCCAA CCTGGTAAAA AATTTCAAGC TGGTGAAATG CAGCATCTAC 
ACGGATATAC AGGAGATTAT CAGCTCAATA CTGCTGAGCG GTCGGATAGG GCCGGACATC 
AAACACGTTG AGTGTTACGG TCTGCGTCTG AAGCATCTGA AATCCGATGA GATCCATTGG 
TTGCACCCTG AGCTAACTGT AGGGGAGGTC CAGGAGAAGT ACGAGTGCCT CCATCTTGAG 
GCTGAGTGGA GGTATGACCT GCGGATCCGT TACCTGCCCG AAGACTTCAA TGAAAGTTTT 
AAAAAGGACA AAACTACCTT GTTATATTTC TATCAGCAGC TCAGAAATGA CTACATGCAA 
CAATACGCAA CGAAAGTGAG CGAAGGCATG GCCTTGCAGC TGGGATGCCT GGAACTCAGG 
AGGTTTTATA AAGATATGCC CCAGAACGCT CTCGAGAAGA AATCCAACTT CGAATTTTTA 
GAGAAGGAAG TGGGACTGGA TTTGTTCTTC CCAAAACAGA TGCAGGAGAG TTTGAAGCCT 
AAACAATTCC GCAAGATGAT CCAGCAGACA TTCCAGCAAT ACGGATCCCT CAAGGAAGAG 
GAATGCATTA CGCGCTTTAT GAACACCCTT TCCACTTTCA CCTCCATCGA TCAAGAAACC 
TACAGATGTG AATTAGCGCA AGGATGGAGC ATCACTGTTG ACTTGGTTAT AGGCCCCAAA 
GGCATTCGGC AGCTGACTAC CAAGGACTCT AAGCCGACAT GCTTTGCCGA ATTCAAGCAG 
ATAAAGTCCA TCAAGTGCTC GTTGCTAGAA GATGGCTGGG CCTTGCTGTT ACTGGGTATA 
TCGGGCGCCC CACAGCCTTT AGCCATAAAA ACATCGTCGT TGGCAGTGGC GGAAAACATG 
GCCGACCTCA TCGACGGTTA CTGCCGGCTG CAAAACGAGT CGAAAGATTC GCTCATTGTC 
AACCCCAAGA AAGATAATGA GAAGAGGATC AGCCTGCCCC AGATCCCAAC TGAAGAAGTA 
CAAGTGCGGC GATCCAGGCT AGTGGAGGGG AACAGTATGG AGTCAGACAT ATACGCAGAG 
ATTCCGGAGG TGACTGAGAC AAAGCTTACA AATAACCGTT TCACCATCAG CCGAAGCGAC 
GTGGCGCTGG GGAGGATCCT GGGAGAAGGC TTTTTTGGGG AGGTCTACGA TGGCGTCTAT 
GTGAAACCGA AGGGCGACAA GATCAACGTG GCGGTAAAGA CCTGTAAGAA TGACTGCGCC 
GCCGACGTCA AGGAGAAATT CATGAGTGAA GCGTATCTGA TGAGGAACTT GGACCACCCG 
CATATAGTAA AACTAATTGG CATCATTGAA GACGACCCAA TATGGATTGT GATGGAGCTG 
TACCCGTACG GAGAGCTTGG GCAGTACCTA GAGAAAAACA AGAACTCCCT GCAGGTTCTC 
AGCCTCATCC TCTACTCACT ACAGATCTGC AAAGCCCTCG CCTACTTAGA GGGAATAAAC 
TGCGTCCACA GGGACATTGC TGTACGGAAC ATTCTGGTAG CTTCTCCTGA GTGTGTGAAA 
CTGGGAGACT TTGGCCTTTC TCGGTACCTA GAGGAGGAAG AGTATTATAA AGCTTCAGTC 
ACCCGGCTGC CAATCAAGTG GATGGCTCCG GAGTCCATTA ATTTCCGGCG GTTCACCTCG 
GCCAGCGATG TCTGGATGTT TGGAGTCTGT ATGTGGGAGA TACTCTCCTT TGGGAAGCAG 
CCATTTTTCT GGCTGGAGAA CAAAGACGTG ATCAGCGTAA TCGAGAAGGG AGATCGCCTG 
CCCAAACCCG AGATGTGCCC GCCGACCCTC TACACCCTGA TGACGCGCTG CTGGACTTAT 
GACCCTTTCG AGAGGCCGAA ATTCACCGAG CTTGTTTGTA GCCTCAGTGA TATTTATCAG 
ACAGAAAAAG AGATCATCAA AGATAAAGAG AGGAACAACC GCCAGCGGCC TCCTAAAATC 
ATTGAGCCTC CTCCCATGCA AGAACCTCCC CCAAAGCCTA GCCGGCCCAA ATACAAGGCA 
GCCGCGCCCG GAAATCTGCT GGCACCTAAC CTACTGACCC AGGTACCAGA GAGTCTATGT 
GCCAGCTCTC CTACTCTCTG TAGCCCAACT GACTACCAGT CTCCACTGGA TTCCACCCGC 
AGCCCACCAC TGCCGCGCCA TAGCATCTTC AAACGCCGCA GCATGAGGGA GGAAGACTTT 
GTAAAACCGA ACAGCAAAGA AGAAGCTCAG AAACTGTGGG AAAAGGAAAG GGACAAAATG 
CTACAGGTCT TAAACCGACA GCAACAACAG ATGGAGGAGG ACGGCAAGTG GTTGAAGCAG 
GAGGAGAAGT CACTGGCCAA TACTCTCAAG GATTCAAAGC AAAAAACAAA CAACAACAAG 
CCCCCTGATG CACTTCCAGA GAAAGAACTG GCGTACACTG AATTCACAGT CCCCCCACAG 
AAGCCTCCCA GGTTAGGGGC ACAGTCCCTA CAACCCCTAC AGCCCACACC TACGGCCAAC 
ATTGACCGGA CAGATGACGG TGTGTACCAG AGTGTGATGG AGCTGGTAAA GGCGGTTCTG 
CAACTGAAGA ATGACATCTA CCAGCTCCCT CCGGAGGGAT ACGTTGCCGT TGTTAAGAAT 
GTGGGGCTGA CCCTTAGAAA ACTCATTGGA AGCGTCGATG AGATCCTTCC TTCTCTCCCA 
CAGAATTCCC GCACTGAGAT TGAAGGGACC CAGAAGCTCC TAAGCAAAGA CCTGGCGGAG 
TTAATCAACA AGATGCGCCT GGCCCAACAG AACGCCGTGA CGTCGCTGAG CGAAGAGTGC 
AAGAAGCAGA TGCTGACGGC GTCGCACACC ATGGCCGTAG ACGCCAAAAA TTTGCTGGAT 
GCCGTGGACC AGGCCAGGGT CAGGGCCAAT TTAGCCAAGG TGGCCGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptk2b ( XM_012964038.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819492.1 Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X3, mRNA.

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CloneID	OXa27045
Clone ID Related Accession (Same CDS sequence)	XM_012964038.2
Accession Version	XM_012964038.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein-tyrosine kinase 2-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGG ACCATGCCAA CCGGACCAAG GGGGGCACAT TTAACCGGCC AGATGGCCCG 
GCGGAGGTGC CGGCGGAAGT GGAGATCAGT AAGGAGGAGA TGAGAATCCT GAAAGTGTGC 
TTCCACAGTA ACAGCTCCAA CCTGGTAAAA AATTTCAAGC TGGTGAAATG CAGCATCTAC 
ACGGATATAC AGGAGATTAT CAGCTCAATA CTGCTGAGCG GTCGGATAGG GCCGGACATC 
AAACACGTTG AGTGTTACGG TCTGCGTCTG AAGCATCTGA AATCCGATGA GATCCATTGG 
TTGCACCCTG AGCTAACTGT AGGGGAGGTC CAGGAGAAGT ACGAGTGCCT CCATCTTGAG 
GCTGAGTGGA GGTATGACCT GCGGATCCGT TACCTGCCCG AAGACTTCAA TGAAAGTTTT 
AAAAAGGACA AAACTACCTT GTTATATTTC TATCAGCAGC TCAGAAATGA CTACATGCAA 
CAATACGCAA CGAAAGTGAG CGAAGGCATG GCCTTGCAGC TGGGATGCCT GGAACTCAGG 
AGGTTTTATA AAGATATGCC CCAGAACGCT CTCGAGAAGA AATCCAACTT CGAATTTTTA 
GAGAAGGAAG TGGGACTGGA TTTGTTCTTC CCAAAACAGA TGCAGGAGAG TTTGAAGCCT 
AAACAATTCC GCAAGATGAT CCAGCAGACA TTCCAGCAAT ACGGATCCCT CAAGGAAGAG 
GAATGCATTA CGCGCTTTAT GAACACCCTT TCCACTTTCA CCTCCATCGA TCAAGAAACC 
TACAGATGTG AATTAGCGCA AGGATGGAGC ATCACTGTTG ACTTGGTTAT AGGCCCCAAA 
GGCATTCGGC AGCTGACTAC CAAGGACTCT AAGCCGACAT GCTTTGCCGA ATTCAAGCAG 
ATAAAGTCCA TCAAGTGCTC GTTGCTAGAA GATGGCTGGG CCTTGCTGTT ACTGGGTATA 
TCGGGCGCCC CACAGCCTTT AGCCATAAAA ACATCGTCGT TGGCAGTGGC GGAAAACATG 
GCCGACCTCA TCGACGGTTA CTGCCGGCTG CAAAACGAGT CGAAAGATTC GCTCATTGTC 
AACCCCAAGA AAGATAATGA GAAGAGGATC AGCCTGCCCC AGATCCCAAC TGAAGAAGTA 
CAAGTGCGGC GATCCAGGCT AGTGGAGGGG AACAGTATGG AGTCAGACAT ATACGCAGAG 
ATTCCGGAGG TGACTGAGAC AAAGCTTACA AATAACCGTT TCACCATCAG CCGAAGCGAC 
GTGGCGCTGG GGAGGATCCT GGGAGAAGGC TTTTTTGGGG AGGTCTACGA TGGCGTCTAT 
GTGAAACCGA AGGGCGACAA GATCAACGTG GCGGTAAAGA CCTGTAAGAA TGACTGCGCC 
GCCGACGTCA AGGAGAAATT CATGAGTGAA GCGTATCTGA TGAGGAACTT GGACCACCCG 
CATATAGTAA AACTAATTGG CATCATTGAA GACGACCCAA TATGGATTGT GATGGAGCTG 
TACCCGTACG GAGAGCTTGG GCAGTACCTA GAGAAAAACA AGAACTCCCT GCAGGTTCTC 
AGCCTCATCC TCTACTCACT ACAGATCTGC AAAGCCCTCG CCTACTTAGA GGGAATAAAC 
TGCGTCCACA GGGACATTGC TGTACGGAAC ATTCTGGTAG CTTCTCCTGA GTGTGTGAAA 
CTGGGAGACT TTGGCCTTTC TCGGTACCTA GAGGAGGAAG AGTATTATAA AGCTTCAGTC 
ACCCGGCTGC CAATCAAGTG GATGGCTCCG GAGTCCATTA ATTTCCGGCG GTTCACCTCG 
GCCAGCGATG TCTGGATGTT TGGAGTCTGT ATGTGGGAGA TACTCTCCTT TGGGAAGCAG 
CCATTTTTCT GGCTGGAGAA CAAAGACGTG ATCAGCGTAA TCGAGAAGGG AGATCGCCTG 
CCCAAACCCG AGATGTGCCC GCCGACCCTC TACACCCTGA TGACGCGCTG CTGGACTTAT 
GACCCTTTCG AGAGGCCGAA ATTCACCGAG CTTGTTTGTA GCCTCAGTGA TATTTATCAG 
ACAGAAAAAG AGATCATCAA AGATAAAGAG AGGAACAACC GCCAGCGGCC TCCTAAAATC 
ATTGAGCCTC CTCCCATGCA AGAACCTCCC CCAAAGCCTA GCCGGCCCAA ATACAAGGCA 
GCCGCGCCCG GAAATCTGCT GGCACCTAAC CTACTGACCC AGGTACCAGA GAGTCTATGT 
GCCAGCTCTC CTACTCTCTG TAGCCCAACT GACTACCAGT CTCCACTGGA TTCCACCCGC 
AGCCCACCAC TGCCGCGCCA TAGCATCTTC AAACGCCGCA GCATGAGGGA GGAAGACTTT 
GTAAAACCGA ACAGCAAAGA AGAAGCTCAG AAACTGTGGG AAAAGGAAAG GGACAAAATG 
CTACAGGTCT TAAACCGACA GCAACAACAG ATGGAGGAGG ACGGCAAGTG GTTGAAGCAG 
GAGGAGAAGT CACTGGCCAA TACTCTCAAG GCACTTCCAG AGAAAGAACT GGCGTACACT 
GAATTCACAG TCCCCCCACA GAAGCCTCCC AGGTTAGGGG CACAGTCCCT ACAACCCCTA 
CAGCCCACAC CTACGGCCAA CATTGACCGG ACAGATGACG GTGTGTACCA GAGTGTGATG 
GAGCTGGTAA AGGCGGTTCT GCAACTGAAG AATGACATCT ACCAGCTCCC TCCGGAGGGA 
TACGTTGCCG TTGTTAAGAA TGTGGGGCTG ACCCTTAGAA AACTCATTGG AAGCGTCGAT 
GAGATCCTTC CTTCTCTCCC ACAGAATTCC CGCACTGAGA TTGAAGGGAC CCAGAAGCTC 
CTAAGCAAAG ACCTGGCGGA GTTAATCAAC AAGATGCGCC TGGCCCAACA GAACGCCGTG 
ACGTCGCTGA GCGAAGAGTG CAAGAAGCAG ATGCTGACGG CGTCGCACAC CATGGCCGTA 
GACGCCAAAA ATTTGCTGGA TGCCGTGGAC CAGGCCAGGG TCAGGGCCAA TTTAGCCAAG 
GTGGCCGCTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819492.1
CDS	195..3209
Translation	MSLDHANRTKGGTFNRPDGPAEVPAEVEISKEEMRILKVCFHSNSSNLVKNFKLVKCSIYTDIQEIISSILLSGRIGPDIKHVECYGLRLKHLKSDEIHWLHPELTVGEVQEKYECLHLEAEWRYDLRIRYLPEDFNESFKKDKTTLLYFYQQLRNDYMQQYATKVSEGMALQLGCLELRRFYKDMPQNALEKKSNFEFLEKEVGLDLFFPKQMQESLKPKQFRKMIQQTFQQYGSLKEEECITRFMNTLSTFTSIDQETYRCELAQGWSITVDLVIGPKGIRQLTTKDSKPTCFAEFKQIKSIKCSLLEDGWALLLLGISGAPQPLAIKTSSLAVAENMADLIDGYCRLQNESKDSLIVNPKKDNEKRISLPQIPTEEVQVRRSRLVEGNSMESDIYAEIPEVTETKLTNNRFTISRSDVALGRILGEGFFGEVYDGVYVKPKGDKINVAVKTCKNDCAADVKEKFMSEAYLMRNLDHPHIVKLIGIIEDDPIWIVMELYPYGELGQYLEKNKNSLQVLSLILYSLQICKALAYLEGINCVHRDIAVRNILVASPECVKLGDFGLSRYLEEEEYYKASVTRLPIKWMAPESINFRRFTSASDVWMFGVCMWEILSFGKQPFFWLENKDVISVIEKGDRLPKPEMCPPTLYTLMTRCWTYDPFERPKFTELVCSLSDIYQTEKEIIKDKERNNRQRPPKIIEPPPMQEPPPKPSRPKYKAAAPGNLLAPNLLTQVPESLCASSPTLCSPTDYQSPLDSTRSPPLPRHSIFKRRSMREEDFVKPNSKEEAQKLWEKERDKMLQVLNRQQQQMEEDGKWLKQEEKSLANTLKALPEKELAYTEFTVPPQKPPRLGAQSLQPLQPTPTANIDRTDDGVYQSVMELVKAVLQLKNDIYQLPPEGYVAVVKNVGLTLRKLIGSVDEILPSLPQNSRTEIEGTQKLLSKDLAELINKMRLAQQNAVTSLSEECKKQMLTASHTMAVDAKNLLDAVDQARVRANLAKVAAE

Target ORF information:

RefSeq Version	XM_012964038.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine kinase 2 beta (ptk2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964038.2

ORF Insert Sequence:

ATGTCGCTGG ACCATGCCAA CCGGACCAAG GGGGGCACAT TTAACCGGCC AGATGGCCCG 
GCGGAGGTGC CGGCGGAAGT GGAGATCAGT AAGGAGGAGA TGAGAATCCT GAAAGTGTGC 
TTCCACAGTA ACAGCTCCAA CCTGGTAAAA AATTTCAAGC TGGTGAAATG CAGCATCTAC 
ACGGATATAC AGGAGATTAT CAGCTCAATA CTGCTGAGCG GTCGGATAGG GCCGGACATC 
AAACACGTTG AGTGTTACGG TCTGCGTCTG AAGCATCTGA AATCCGATGA GATCCATTGG 
TTGCACCCTG AGCTAACTGT AGGGGAGGTC CAGGAGAAGT ACGAGTGCCT CCATCTTGAG 
GCTGAGTGGA GGTATGACCT GCGGATCCGT TACCTGCCCG AAGACTTCAA TGAAAGTTTT 
AAAAAGGACA AAACTACCTT GTTATATTTC TATCAGCAGC TCAGAAATGA CTACATGCAA 
CAATACGCAA CGAAAGTGAG CGAAGGCATG GCCTTGCAGC TGGGATGCCT GGAACTCAGG 
AGGTTTTATA AAGATATGCC CCAGAACGCT CTCGAGAAGA AATCCAACTT CGAATTTTTA 
GAGAAGGAAG TGGGACTGGA TTTGTTCTTC CCAAAACAGA TGCAGGAGAG TTTGAAGCCT 
AAACAATTCC GCAAGATGAT CCAGCAGACA TTCCAGCAAT ACGGATCCCT CAAGGAAGAG 
GAATGCATTA CGCGCTTTAT GAACACCCTT TCCACTTTCA CCTCCATCGA TCAAGAAACC 
TACAGATGTG AATTAGCGCA AGGATGGAGC ATCACTGTTG ACTTGGTTAT AGGCCCCAAA 
GGCATTCGGC AGCTGACTAC CAAGGACTCT AAGCCGACAT GCTTTGCCGA ATTCAAGCAG 
ATAAAGTCCA TCAAGTGCTC GTTGCTAGAA GATGGCTGGG CCTTGCTGTT ACTGGGTATA 
TCGGGCGCCC CACAGCCTTT AGCCATAAAA ACATCGTCGT TGGCAGTGGC GGAAAACATG 
GCCGACCTCA TCGACGGTTA CTGCCGGCTG CAAAACGAGT CGAAAGATTC GCTCATTGTC 
AACCCCAAGA AAGATAATGA GAAGAGGATC AGCCTGCCCC AGATCCCAAC TGAAGAAGTA 
CAAGTGCGGC GATCCAGGCT AGTGGAGGGG AACAGTATGG AGTCAGACAT ATACGCAGAG 
ATTCCGGAGG TGACTGAGAC AAAGCTTACA AATAACCGTT TCACCATCAG CCGAAGCGAC 
GTGGCGCTGG GGAGGATCCT GGGAGAAGGC TTTTTTGGGG AGGTCTACGA TGGCGTCTAT 
GTGAAACCGA AGGGCGACAA GATCAACGTG GCGGTAAAGA CCTGTAAGAA TGACTGCGCC 
GCCGACGTCA AGGAGAAATT CATGAGTGAA GCGTATCTGA TGAGGAACTT GGACCACCCG 
CATATAGTAA AACTAATTGG CATCATTGAA GACGACCCAA TATGGATTGT GATGGAGCTG 
TACCCGTACG GAGAGCTTGG GCAGTACCTA GAGAAAAACA AGAACTCCCT GCAGGTTCTC 
AGCCTCATCC TCTACTCACT ACAGATCTGC AAAGCCCTCG CCTACTTAGA GGGAATAAAC 
TGCGTCCACA GGGACATTGC TGTACGGAAC ATTCTGGTAG CTTCTCCTGA GTGTGTGAAA 
CTGGGAGACT TTGGCCTTTC TCGGTACCTA GAGGAGGAAG AGTATTATAA AGCTTCAGTC 
ACCCGGCTGC CAATCAAGTG GATGGCTCCG GAGTCCATTA ATTTCCGGCG GTTCACCTCG 
GCCAGCGATG TCTGGATGTT TGGAGTCTGT ATGTGGGAGA TACTCTCCTT TGGGAAGCAG 
CCATTTTTCT GGCTGGAGAA CAAAGACGTG ATCAGCGTAA TCGAGAAGGG AGATCGCCTG 
CCCAAACCCG AGATGTGCCC GCCGACCCTC TACACCCTGA TGACGCGCTG CTGGACTTAT 
GACCCTTTCG AGAGGCCGAA ATTCACCGAG CTTGTTTGTA GCCTCAGTGA TATTTATCAG 
ACAGAAAAAG AGATCATCAA AGATAAAGAG AGGAACAACC GCCAGCGGCC TCCTAAAATC 
ATTGAGCCTC CTCCCATGCA AGAACCTCCC CCAAAGCCTA GCCGGCCCAA ATACAAGGCA 
GCCGCGCCCG GAAATCTGCT GGCACCTAAC CTACTGACCC AGGTACCAGA GAGTCTATGT 
GCCAGCTCTC CTACTCTCTG TAGCCCAACT GACTACCAGT CTCCACTGGA TTCCACCCGC 
AGCCCACCAC TGCCGCGCCA TAGCATCTTC AAACGCCGCA GCATGAGGGA GGAAGACTTT 
GTAAAACCGA ACAGCAAAGA AGAAGCTCAG AAACTGTGGG AAAAGGAAAG GGACAAAATG 
CTACAGGTCT TAAACCGACA GCAACAACAG ATGGAGGAGG ACGGCAAGTG GTTGAAGCAG 
GAGGAGAAGT CACTGGCCAA TACTCTCAAG GCACTTCCAG AGAAAGAACT GGCGTACACT 
GAATTCACAG TCCCCCCACA GAAGCCTCCC AGGTTAGGGG CACAGTCCCT ACAACCCCTA 
CAGCCCACAC CTACGGCCAA CATTGACCGG ACAGATGACG GTGTGTACCA GAGTGTGATG 
GAGCTGGTAA AGGCGGTTCT GCAACTGAAG AATGACATCT ACCAGCTCCC TCCGGAGGGA 
TACGTTGCCG TTGTTAAGAA TGTGGGGCTG ACCCTTAGAA AACTCATTGG AAGCGTCGAT 
GAGATCCTTC CTTCTCTCCC ACAGAATTCC CGCACTGAGA TTGAAGGGAC CCAGAAGCTC 
CTAAGCAAAG ACCTGGCGGA GTTAATCAAC AAGATGCGCC TGGCCCAACA GAACGCCGTG 
ACGTCGCTGA GCGAAGAGTG CAAGAAGCAG ATGCTGACGG CGTCGCACAC CATGGCCGTA 
GACGCCAAAA ATTTGCTGGA TGCCGTGGAC CAGGCCAGGG TCAGGGCCAA TTTAGCCAAG 
GTGGCCGCTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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ptk2b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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