sema3fl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema3fl
Entrez Gene ID	448771
Full Name	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012968258.2	XP_012823712.1	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F isoform X2
XM_012968259.2	XP_012823713.1	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F isoform X3
XM_012968257.2	XP_012823711.1	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F isoform X1
NM_001006923.1	NP_001006924.1	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F precursor
XM_012968259.3	XP_012823713.1	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F isoform X3
XM_012968257.3	XP_012823711.1	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F isoform X1
XM_012968258.3	XP_012823712.1	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	sema3ga	NP_001014823.1
Xenopus tropicalis (tropical clawed frog)	sema3f	NP_001006924.1
Danio rerio (zebrafish)	sema3gb	NP_001014824.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sema3fl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema3fl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa23710	NM_001006923.1 Latest version!	Xenopus tropicalis semaphorin 3F like (sema3fl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa23710	XM_012968259.3 Latest version!	Xenopus tropicalis semaphorin 3F like (sema3fl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa26787	XM_012968257.3 Latest version!	Xenopus tropicalis semaphorin 3F like (sema3fl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa26788	XM_012968258.3 Latest version!	Xenopus tropicalis semaphorin 3F like (sema3fl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa23710	XM_012968259.2	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (sema3f), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa26787	XM_012968257.2	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (sema3f), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa26788	XM_012968258.2	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (sema3f), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema3fl ( NM_001006923.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001006924.1 Xenopus tropicalis semaphorin 3F like (sema3fl), mRNA.

Price & Availability↑

CloneID	OXa23710
Clone ID Related Accession (Same CDS sequence)	XM_012968259.2 , NM_001006923.1 , XM_012968259.3
Accession Version	NM_001006923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076684.1. ##Evidence-Data-START## Transcript exon combination :: BC076684.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028292, SAMD00028293 [ECO:0000348] ##Evidence-Data-END##

ATGCTGGTAT CGGCATTACC TCCTCTGCTT CTGCTGCTTC TCAACTTGCC CCCGTGTGTG 
CGAACGACGC GGCCCCCTCG CGTGTACCTT TCATACAGAG ATCTCATGGA ATCCCGCTCT 
GCGCGCTCCT TTACTTTTGG GTTTAACACC AGCGATTACC GTATTCTTCT CCTTGATCAG 
GACCAGGACC GGATGTACGT GGGAAGCCGC GATTATGTGC TGTCTCTTGA CCTCGGAAAC 
ATCAACCGGG AGCCCCTAAT TATTCATTGG CCAGCTCCAC TGAACCGTCA CAAGGAATGC 
CTCATGGCAG GGAGGGGGCG TGTAGACGAA TGCTACAATT ACCTGCGTGT CATTCAGCCA 
CTGAACCGTA CCCATCTCTA TGCGTGTGGC ACAGGAGCCT ACCAGCCACA GTGTGCTGTT 
ATCTACAGGG GTTGGAGATC AGAGGATTAT GTGTTCCGCA TTGTCCCTGC CTCGCTGGAG 
TCAGGAAAGG GAAGGTGCCC TTATGATCCC AAGCAGGAAA ACATGGCGAC CTTAATAGAT 
GGCGTGTTGT ATGCAGGGGT CCAGGTGGAT TACATGGGGA CGGACTCTGC CATTTTCCGG 
ACAATGGGGC ACAAGCCCCC AATACGCACG GAGCAGTACG ACTCTCGATG GCTGAATGAT 
CCGTTATTTG TGGGGTCCCT GGTGGTCTCA GAAAGCAGCT TCAAAGAGGA TGACAAACTG 
TACATCTTAT TCCGGGAGAG AAGCCTAGAA GGGGAGAATG GCCCGGCGTT GGTGTCTCGT 
ATTGCTCGTG TCTGCTTGAA TGACGAGGGG GGTCTGCGCT CTCTGGTGGG AAAGTGGACT 
TCCTATCTCA AGGCCCGGCT AGTGTGCTCC GTGATTGGCC AGGATGGTGT GGAGACATCG 
TTTGACCAAC TCCGTGACGT CTTTATCCAG CCGACCCACG ACAAACAAAA CCCGCTGATC 
TACGGAGTCT TCACTACACT GGGTTCCGTG TTCCGGGGCT CCGCCGTCTG CGTTTACTCT 
CTGGCTGATG TCCGTGCTGT GTTTAATGGA CCCTTTGCAC ACAAGGAGGG ACATGGGTAC 
CAGATGACTG CCTACTCCGG GAAAACACCG TATCCCAGAC CTGGAGCGTG CGCTGGCGGA 
TTCTCCGTCC CCAGGAATCG ATCGAGCAGG AACTTTGGGG AGGATGTTTT GAGGTTTGTG 
CGCACTCACC CCCTGATGTA CAGCTCCGTG TACCCCGTCA ACCGCAGACC CCTACTTTTG 
CTCTCAGATG CCTCCTACAC CTACACCAGC ATTGCTGTGG ACAGAGTGCC CGCGGCTGAT 
GGGGAGTATA CCGTCCTGTT CCTGGGCACA GACCGTGGTA CTGTTCAGAA AGTAATGATC 
CTGCCCCGGG GCACCAAGGA AACAGAAAGT ATCACCTTGG AGGAAGTAGA AGTGTTCAAG 
GTGCCATCTC CTATCAGAAA CCTCAGGATA TCGTCAAAGA GGCATCAGCT CTACGTCTCC 
TCAGATGTCG GGGTCACCCA GCTGTCTCTT CATCGTTGTG GTCGTTATGG AGATTCCTGC 
GCTGATTGCT GTCTGGCCAG AGACCCATAT TGTGCCTGGG ATGGAAAGGG CTGTGTGCGT 
TACACCCCTT CACCCAGCAG GAGGAGTCGG CGCCAGGATG TTAAACATGG AGACCCCTTA 
AGGCAATGCC GGGGATACAA TACACAAGTG GATCGAGGCA TTGCAGAGAG GCTGCAGATC 
AGCGTGGAAG GAGGGAGTGC ATACCTTCAG TGCGACACCC ACTCCCCACT TGAGAGCGTC 
ACCTGGTTAC TGCAAAGAGA TGGCTCCCAG CATCGGCGAG AGGTGCGACT CTCGGCCATT 
GATGGGGGCG CAGTGCTACG CTCAGTACAA GCCAGCGACG CGGGGGTCTA TACCTGCACG 
GGTACGGAGA ACGGTTTCCG GCGATCACGT GCAAAAATAC GGCTCAATGT TCTGCCACGG 
GACCTACTAG AAAAACTAAC AGCTGCGCCA ACCCTTGCTC CAATGGCAGC ACAATGCCCA 
CCGCCCCGCA GACCAAGACC GCGGCCATCT CACACAGACC GTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006924.1
CDS	265..2352
Translation	MLVSALPPLLLLLLNLPPCVRTTRPPRVYLSYRDLMESRSARSFTFGFNTSDYRILLLDQDQDRMYVGSRDYVLSLDLGNINREPLIIHWPAPLNRHKECLMAGRGRVDECYNYLRVIQPLNRTHLYACGTGAYQPQCAVIYRGWRSEDYVFRIVPASLESGKGRCPYDPKQENMATLIDGVLYAGVQVDYMGTDSAIFRTMGHKPPIRTEQYDSRWLNDPLFVGSLVVSESSFKEDDKLYILFRERSLEGENGPALVSRIARVCLNDEGGLRSLVGKWTSYLKARLVCSVIGQDGVETSFDQLRDVFIQPTHDKQNPLIYGVFTTLGSVFRGSAVCVYSLADVRAVFNGPFAHKEGHGYQMTAYSGKTPYPRPGACAGGFSVPRNRSSRNFGEDVLRFVRTHPLMYSSVYPVNRRPLLLLSDASYTYTSIAVDRVPAADGEYTVLFLGTDRGTVQKVMILPRGTKETESITLEEVEVFKVPSPIRNLRISSKRHQLYVSSDVGVTQLSLHRCGRYGDSCADCCLARDPYCAWDGKGCVRYTPSPSRRSRRQDVKHGDPLRQCRGYNTQVDRGIAERLQISVEGGSAYLQCDTHSPLESVTWLLQRDGSQHRREVRLSAIDGGAVLRSVQASDAGVYTCTGTENGFRRSRAKIRLNVLPRDLLEKLTAAPTLAPMAAQCPPPRRPRPRPSHTDRN

Target ORF information:

RefSeq Version	NM_001006923.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis semaphorin 3F like (sema3fl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006923.1

ORF Insert Sequence:

ATGCTGGTAT CGGCATTACC TCCTCTGCTT CTGCTGCTTC TCAACTTGCC CCCGTGTGTG 
CGAACGACGC GGCCCCCTCG CGTGTACCTT TCATACAGAG ATCTCATGGA ATCCCGCTCT 
GCGCGCTCCT TTACTTTTGG GTTTAACACC AGCGATTACC GTATTCTTCT CCTTGATCAG 
GACCAGGACC GGATGTACGT GGGAAGCCGC GATTATGTGC TGTCTCTTGA CCTCGGAAAC 
ATCAACCGGG AGCCCCTAAT TATTCATTGG CCAGCTCCAC TGAACCGTCA CAAGGAATGC 
CTCATGGCAG GGAGGGGGCG TGTAGACGAA TGCTACAATT ACCTGCGTGT CATTCAGCCA 
CTGAACCGTA CCCATCTCTA TGCGTGTGGC ACAGGAGCCT ACCAGCCACA GTGTGCTGTT 
ATCTACAGGG GTTGGAGATC AGAGGATTAT GTGTTCCGCA TTGTCCCTGC CTCGCTGGAG 
TCAGGAAAGG GAAGGTGCCC TTATGATCCC AAGCAGGAAA ACATGGCGAC CTTAATAGAT 
GGCGTGTTGT ATGCAGGGGT CCAGGTGGAT TACATGGGGA CGGACTCTGC CATTTTCCGG 
ACAATGGGGC ACAAGCCCCC AATACGCACG GAGCAGTACG ACTCTCGATG GCTGAATGAT 
CCGTTATTTG TGGGGTCCCT GGTGGTCTCA GAAAGCAGCT TCAAAGAGGA TGACAAACTG 
TACATCTTAT TCCGGGAGAG AAGCCTAGAA GGGGAGAATG GCCCGGCGTT GGTGTCTCGT 
ATTGCTCGTG TCTGCTTGAA TGACGAGGGG GGTCTGCGCT CTCTGGTGGG AAAGTGGACT 
TCCTATCTCA AGGCCCGGCT AGTGTGCTCC GTGATTGGCC AGGATGGTGT GGAGACATCG 
TTTGACCAAC TCCGTGACGT CTTTATCCAG CCGACCCACG ACAAACAAAA CCCGCTGATC 
TACGGAGTCT TCACTACACT GGGTTCCGTG TTCCGGGGCT CCGCCGTCTG CGTTTACTCT 
CTGGCTGATG TCCGTGCTGT GTTTAATGGA CCCTTTGCAC ACAAGGAGGG ACATGGGTAC 
CAGATGACTG CCTACTCCGG GAAAACACCG TATCCCAGAC CTGGAGCGTG CGCTGGCGGA 
TTCTCCGTCC CCAGGAATCG ATCGAGCAGG AACTTTGGGG AGGATGTTTT GAGGTTTGTG 
CGCACTCACC CCCTGATGTA CAGCTCCGTG TACCCCGTCA ACCGCAGACC CCTACTTTTG 
CTCTCAGATG CCTCCTACAC CTACACCAGC ATTGCTGTGG ACAGAGTGCC CGCGGCTGAT 
GGGGAGTATA CCGTCCTGTT CCTGGGCACA GACCGTGGTA CTGTTCAGAA AGTAATGATC 
CTGCCCCGGG GCACCAAGGA AACAGAAAGT ATCACCTTGG AGGAAGTAGA AGTGTTCAAG 
GTGCCATCTC CTATCAGAAA CCTCAGGATA TCGTCAAAGA GGCATCAGCT CTACGTCTCC 
TCAGATGTCG GGGTCACCCA GCTGTCTCTT CATCGTTGTG GTCGTTATGG AGATTCCTGC 
GCTGATTGCT GTCTGGCCAG AGACCCATAT TGTGCCTGGG ATGGAAAGGG CTGTGTGCGT 
TACACCCCTT CACCCAGCAG GAGGAGTCGG CGCCAGGATG TTAAACATGG AGACCCCTTA 
AGGCAATGCC GGGGATACAA TACACAAGTG GATCGAGGCA TTGCAGAGAG GCTGCAGATC 
AGCGTGGAAG GAGGGAGTGC ATACCTTCAG TGCGACACCC ACTCCCCACT TGAGAGCGTC 
ACCTGGTTAC TGCAAAGAGA TGGCTCCCAG CATCGGCGAG AGGTGCGACT CTCGGCCATT 
GATGGGGGCG CAGTGCTACG CTCAGTACAA GCCAGCGACG CGGGGGTCTA TACCTGCACG 
GGTACGGAGA ACGGTTTCCG GCGATCACGT GCAAAAATAC GGCTCAATGT TCTGCCACGG 
GACCTACTAG AAAAACTAAC AGCTGCGCCA ACCCTTGCTC CAATGGCAGC ACAATGCCCA 
CCGCCCCGCA GACCAAGACC GCGGCCATCT CACACAGACC GTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fl ( XM_012968259.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823713.1 Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (sema3f), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa23710
Clone ID Related Accession (Same CDS sequence)	XM_012968259.2 , NM_001006923.1 , XM_012968259.3
Accession Version	XM_012968259.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTAT CGGCATTACC TCCTCTGCTT CTGCTGCTTC TCAACTTGCC CCCGTGTGTG 
CGAACGACGC GGCCCCCTCG CGTGTACCTT TCATACAGAG ATCTCATGGA ATCCCGCTCT 
GCGCGCTCCT TTACTTTTGG GTTTAACACC AGCGATTACC GTATTCTTCT CCTTGATCAG 
GACCAGGACC GGATGTACGT GGGAAGCCGC GATTATGTGC TGTCTCTTGA CCTCGGAAAC 
ATCAACCGGG AGCCCCTAAT TATTCATTGG CCAGCTCCAC TGAACCGTCA CAAGGAATGC 
CTCATGGCAG GGAGGGGGCG TGTAGACGAA TGCTACAATT ACCTGCGTGT CATTCAGCCA 
CTGAACCGTA CCCATCTCTA TGCGTGTGGC ACAGGAGCCT ACCAGCCACA GTGTGCTGTT 
ATCTACAGGG GTTGGAGATC AGAGGATTAT GTGTTCCGCA TTGTCCCTGC CTCGCTGGAG 
TCAGGAAAGG GAAGGTGCCC TTATGATCCC AAGCAGGAAA ACATGGCGAC CTTAATAGAT 
GGCGTGTTGT ATGCAGGGGT CCAGGTGGAT TACATGGGGA CGGACTCTGC CATTTTCCGG 
ACAATGGGGC ACAAGCCCCC AATACGCACG GAGCAGTACG ACTCTCGATG GCTGAATGAT 
CCGTTATTTG TGGGGTCCCT GGTGGTCTCA GAAAGCAGCT TCAAAGAGGA TGACAAACTG 
TACATCTTAT TCCGGGAGAG AAGCCTAGAA GGGGAGAATG GCCCGGCGTT GGTGTCTCGT 
ATTGCTCGTG TCTGCTTGAA TGACGAGGGG GGTCTGCGCT CTCTGGTGGG AAAGTGGACT 
TCCTATCTCA AGGCCCGGCT AGTGTGCTCC GTGATTGGCC AGGATGGTGT GGAGACATCG 
TTTGACCAAC TCCGTGACGT CTTTATCCAG CCGACCCACG ACAAACAAAA CCCGCTGATC 
TACGGAGTCT TCACTACACT GGGTTCCGTG TTCCGGGGCT CCGCCGTCTG CGTTTACTCT 
CTGGCTGATG TCCGTGCTGT GTTTAATGGA CCCTTTGCAC ACAAGGAGGG ACATGGGTAC 
CAGATGACTG CCTACTCCGG GAAAACACCG TATCCCAGAC CTGGAGCGTG CGCTGGCGGA 
TTCTCCGTCC CCAGGAATCG ATCGAGCAGG AACTTTGGGG AGGATGTTTT GAGGTTTGTG 
CGCACTCACC CCCTGATGTA CAGCTCCGTG TACCCCGTCA ACCGCAGACC CCTACTTTTG 
CTCTCAGATG CCTCCTACAC CTACACCAGC ATTGCTGTGG ACAGAGTGCC CGCGGCTGAT 
GGGGAGTATA CCGTCCTGTT CCTGGGCACA GACCGTGGTA CTGTTCAGAA AGTAATGATC 
CTGCCCCGGG GCACCAAGGA AACAGAAAGT ATCACCTTGG AGGAAGTAGA AGTGTTCAAG 
GTGCCATCTC CTATCAGAAA CCTCAGGATA TCGTCAAAGA GGCATCAGCT CTACGTCTCC 
TCAGATGTCG GGGTCACCCA GCTGTCTCTT CATCGTTGTG GTCGTTATGG AGATTCCTGC 
GCTGATTGCT GTCTGGCCAG AGACCCATAT TGTGCCTGGG ATGGAAAGGG CTGTGTGCGT 
TACACCCCTT CACCCAGCAG GAGGAGTCGG CGCCAGGATG TTAAACATGG AGACCCCTTA 
AGGCAATGCC GGGGATACAA TACACAAGTG GATCGAGGCA TTGCAGAGAG GCTGCAGATC 
AGCGTGGAAG GAGGGAGTGC ATACCTTCAG TGCGACACCC ACTCCCCACT TGAGAGCGTC 
ACCTGGTTAC TGCAAAGAGA TGGCTCCCAG CATCGGCGAG AGGTGCGACT CTCGGCCATT 
GATGGGGGCG CAGTGCTACG CTCAGTACAA GCCAGCGACG CGGGGGTCTA TACCTGCACG 
GGTACGGAGA ACGGTTTCCG GCGATCACGT GCAAAAATAC GGCTCAATGT TCTGCCACGG 
GACCTACTAG AAAAACTAAC AGCTGCGCCA ACCCTTGCTC CAATGGCAGC ACAATGCCCA 
CCGCCCCGCA GACCAAGACC GCGGCCATCT CACACAGACC GTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823713.1
CDS	179..2266
Translation	MLVSALPPLLLLLLNLPPCVRTTRPPRVYLSYRDLMESRSARSFTFGFNTSDYRILLLDQDQDRMYVGSRDYVLSLDLGNINREPLIIHWPAPLNRHKECLMAGRGRVDECYNYLRVIQPLNRTHLYACGTGAYQPQCAVIYRGWRSEDYVFRIVPASLESGKGRCPYDPKQENMATLIDGVLYAGVQVDYMGTDSAIFRTMGHKPPIRTEQYDSRWLNDPLFVGSLVVSESSFKEDDKLYILFRERSLEGENGPALVSRIARVCLNDEGGLRSLVGKWTSYLKARLVCSVIGQDGVETSFDQLRDVFIQPTHDKQNPLIYGVFTTLGSVFRGSAVCVYSLADVRAVFNGPFAHKEGHGYQMTAYSGKTPYPRPGACAGGFSVPRNRSSRNFGEDVLRFVRTHPLMYSSVYPVNRRPLLLLSDASYTYTSIAVDRVPAADGEYTVLFLGTDRGTVQKVMILPRGTKETESITLEEVEVFKVPSPIRNLRISSKRHQLYVSSDVGVTQLSLHRCGRYGDSCADCCLARDPYCAWDGKGCVRYTPSPSRRSRRQDVKHGDPLRQCRGYNTQVDRGIAERLQISVEGGSAYLQCDTHSPLESVTWLLQRDGSQHRREVRLSAIDGGAVLRSVQASDAGVYTCTGTENGFRRSRAKIRLNVLPRDLLEKLTAAPTLAPMAAQCPPPRRPRPRPSHTDRN

Target ORF information:

RefSeq Version	XM_012968259.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (sema3f), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968259.2

ORF Insert Sequence:

ATGCTGGTAT CGGCATTACC TCCTCTGCTT CTGCTGCTTC TCAACTTGCC CCCGTGTGTG 
CGAACGACGC GGCCCCCTCG CGTGTACCTT TCATACAGAG ATCTCATGGA ATCCCGCTCT 
GCGCGCTCCT TTACTTTTGG GTTTAACACC AGCGATTACC GTATTCTTCT CCTTGATCAG 
GACCAGGACC GGATGTACGT GGGAAGCCGC GATTATGTGC TGTCTCTTGA CCTCGGAAAC 
ATCAACCGGG AGCCCCTAAT TATTCATTGG CCAGCTCCAC TGAACCGTCA CAAGGAATGC 
CTCATGGCAG GGAGGGGGCG TGTAGACGAA TGCTACAATT ACCTGCGTGT CATTCAGCCA 
CTGAACCGTA CCCATCTCTA TGCGTGTGGC ACAGGAGCCT ACCAGCCACA GTGTGCTGTT 
ATCTACAGGG GTTGGAGATC AGAGGATTAT GTGTTCCGCA TTGTCCCTGC CTCGCTGGAG 
TCAGGAAAGG GAAGGTGCCC TTATGATCCC AAGCAGGAAA ACATGGCGAC CTTAATAGAT 
GGCGTGTTGT ATGCAGGGGT CCAGGTGGAT TACATGGGGA CGGACTCTGC CATTTTCCGG 
ACAATGGGGC ACAAGCCCCC AATACGCACG GAGCAGTACG ACTCTCGATG GCTGAATGAT 
CCGTTATTTG TGGGGTCCCT GGTGGTCTCA GAAAGCAGCT TCAAAGAGGA TGACAAACTG 
TACATCTTAT TCCGGGAGAG AAGCCTAGAA GGGGAGAATG GCCCGGCGTT GGTGTCTCGT 
ATTGCTCGTG TCTGCTTGAA TGACGAGGGG GGTCTGCGCT CTCTGGTGGG AAAGTGGACT 
TCCTATCTCA AGGCCCGGCT AGTGTGCTCC GTGATTGGCC AGGATGGTGT GGAGACATCG 
TTTGACCAAC TCCGTGACGT CTTTATCCAG CCGACCCACG ACAAACAAAA CCCGCTGATC 
TACGGAGTCT TCACTACACT GGGTTCCGTG TTCCGGGGCT CCGCCGTCTG CGTTTACTCT 
CTGGCTGATG TCCGTGCTGT GTTTAATGGA CCCTTTGCAC ACAAGGAGGG ACATGGGTAC 
CAGATGACTG CCTACTCCGG GAAAACACCG TATCCCAGAC CTGGAGCGTG CGCTGGCGGA 
TTCTCCGTCC CCAGGAATCG ATCGAGCAGG AACTTTGGGG AGGATGTTTT GAGGTTTGTG 
CGCACTCACC CCCTGATGTA CAGCTCCGTG TACCCCGTCA ACCGCAGACC CCTACTTTTG 
CTCTCAGATG CCTCCTACAC CTACACCAGC ATTGCTGTGG ACAGAGTGCC CGCGGCTGAT 
GGGGAGTATA CCGTCCTGTT CCTGGGCACA GACCGTGGTA CTGTTCAGAA AGTAATGATC 
CTGCCCCGGG GCACCAAGGA AACAGAAAGT ATCACCTTGG AGGAAGTAGA AGTGTTCAAG 
GTGCCATCTC CTATCAGAAA CCTCAGGATA TCGTCAAAGA GGCATCAGCT CTACGTCTCC 
TCAGATGTCG GGGTCACCCA GCTGTCTCTT CATCGTTGTG GTCGTTATGG AGATTCCTGC 
GCTGATTGCT GTCTGGCCAG AGACCCATAT TGTGCCTGGG ATGGAAAGGG CTGTGTGCGT 
TACACCCCTT CACCCAGCAG GAGGAGTCGG CGCCAGGATG TTAAACATGG AGACCCCTTA 
AGGCAATGCC GGGGATACAA TACACAAGTG GATCGAGGCA TTGCAGAGAG GCTGCAGATC 
AGCGTGGAAG GAGGGAGTGC ATACCTTCAG TGCGACACCC ACTCCCCACT TGAGAGCGTC 
ACCTGGTTAC TGCAAAGAGA TGGCTCCCAG CATCGGCGAG AGGTGCGACT CTCGGCCATT 
GATGGGGGCG CAGTGCTACG CTCAGTACAA GCCAGCGACG CGGGGGTCTA TACCTGCACG 
GGTACGGAGA ACGGTTTCCG GCGATCACGT GCAAAAATAC GGCTCAATGT TCTGCCACGG 
GACCTACTAG AAAAACTAAC AGCTGCGCCA ACCCTTGCTC CAATGGCAGC ACAATGCCCA 
CCGCCCCGCA GACCAAGACC GCGGCCATCT CACACAGACC GTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fl ( XM_012968257.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823711.1 Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (sema3f), transcript variant X1, mRNA.

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CloneID	OXa26787
Clone ID Related Accession (Same CDS sequence)	XM_012968257.2 , XM_012968257.3
Accession Version	XM_012968257.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTG ACACCATGCT GGTATCGGCA TTACCTCCTC TGCTTCTGCT GCTTCTCAAC 
TTGCCCCCGT GTGTGCGAAC GACGCGGCCC CCTCGCGTGT ACCTTTCATA CAGAGATCTC 
ATGGAATCCC GCTCTGCGCG CTCCTTTACT TTTGGGTTTA ACACCAGCGA TTACCGTATT 
CTTCTCCTTG ATCAGGACCA GGACCGGATG TACGTGGGAA GCCGCGATTA TGTGCTGTCT 
CTTGACCTCG GAAACATCAA CCGGGAGCCC CTAATTATTC ATTGGCCAGC TCCACTGAAC 
CGTCACAAGG AATGCCTCAT GGCAGGGAGG GGGCGTGTAG ACGAATGCTA CAATTACCTG 
CGTGTCATTC AGCCACTGAA CCGTACCCAT CTCTATGCGT GTGGCACAGG AGCCTACCAG 
CCACAGTGTG CTGTTATCTA CAGGGGTTGG AGATCAGAGG ATTATGTGTT CCGCATTGTC 
CCTGCCTCGC TGGAGTCAGG AAAGGGAAGG TGCCCTTATG ATCCCAAGCA GGAAAACATG 
GCGACCTTAA TAGATGGCGT GTTGTATGCA GGGGTCCAGG TGGATTACAT GGGGACGGAC 
TCTGCCATTT TCCGGACAAT GGGGCACAAG CCCCCAATAC GCACGGAGCA GTACGACTCT 
CGATGGCTGA ATGATCCGTT ATTTGTGGGG TCCCTGGTGG TCTCAGAAAG CAGCTTCAAA 
GAGGATGACA AACTGTACAT CTTATTCCGG GAGAGAAGCC TAGAAGGGGA GAATGGCCCG 
GCGTTGGTGT CTCGTATTGC TCGTGTCTGC TTGAATGACG AGGGGGGTCT GCGCTCTCTG 
GTGGGAAAGT GGACTTCCTA TCTCAAGGCC CGGCTAGTGT GCTCCGTGAT TGGCCAGGAT 
GGTGTGGAGA CATCGTTTGA CCAACTCCGT GACGTCTTTA TCCAGCCGAC CCACGACAAA 
CAAAACCCGC TGATCTACGG AGTCTTCACT ACACTGGGTT CCGTGTTCCG GGGCTCCGCC 
GTCTGCGTTT ACTCTCTGGC TGATGTCCGT GCTGTGTTTA ATGGACCCTT TGCACACAAG 
GAGGGACATG GGTACCAGAT GACTGCCTAC TCCGGGAAAA CACCGTATCC CAGACCTGGA 
GCGTGCGCTG GCGGATTCTC CGTCCCCAGG AATCGATCGA GCAGGAACTT TGGGGAGGAT 
GTTTTGAGGT TTGTGCGCAC TCACCCCCTG ATGTACAGCT CCGTGTACCC CGTCAACCGC 
AGACCCCTAC TTTTGCTCTC AGATGCCTCC TACACCTACA CCAGCATTGC TGTGGACAGA 
GTGCCCGCGG CTGATGGGGA GTATACCGTC CTGTTCCTGG GCACAGACCG TGGTACTGTT 
CAGAAAGTAA TGATCCTGCC CCGGGGCACC AAGGAAACAG AAAGTATCAC CTTGGAGGAA 
GTAGAAGTGT TCAAGGTGCC ATCTCCTATC AGAAACCTCA GGATATCGTC AAAGAGGCAT 
CAGCTCTACG TCTCCTCAGA TGTCGGGGTC ACCCAGCTGT CTCTTCATCG TTGTGGTCGT 
TATGGAGATT CCTGCGCTGA TTGCTGTCTG GCCAGAGACC CATATTGTGC CTGGGATGGA 
AAGGGCTGTG TGCGTTACAC CCCTTCACCC AGCAGGAGGA GTCGGCGCCA GGATGTTAAA 
CATGGAGACC CCTTAAGGCA ATGCCGGGGA TACAATACAC AAGTGGATCG AGGCATTGCA 
GAGAGGCTGC AGATCAGCGT GGAAGGAGGG AGTGCATACC TTCAGTGCGA CACCCACTCC 
CCACTTGAGA GCGTCACCTG GTTACTGCAA AGAGATGGCT CCCAGCATCG GCGAGAGGTG 
CGACTCTCGG CCATTGATGG GGGCGCAGTG CTACGCTCAG TACAAGCCAG CGACGCGGGG 
GTCTATACCT GCACGGGTAC GGAGAACGGT TTCCGGCGAT CACGTGCAAA AATACGGCTC 
AATGTTCTGC CACGGGACCT ACTAGAAAAA CTAACAGCTG CGCCAACCCT TGCTCCAATG 
GCAGCACAAT GCCCACCGCC CCGCAGACCA AGACCGCGGC CATCTCACAC AGACCGTAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823711.1
CDS	18..2120
Translation	MAGDTMLVSALPPLLLLLLNLPPCVRTTRPPRVYLSYRDLMESRSARSFTFGFNTSDYRILLLDQDQDRMYVGSRDYVLSLDLGNINREPLIIHWPAPLNRHKECLMAGRGRVDECYNYLRVIQPLNRTHLYACGTGAYQPQCAVIYRGWRSEDYVFRIVPASLESGKGRCPYDPKQENMATLIDGVLYAGVQVDYMGTDSAIFRTMGHKPPIRTEQYDSRWLNDPLFVGSLVVSESSFKEDDKLYILFRERSLEGENGPALVSRIARVCLNDEGGLRSLVGKWTSYLKARLVCSVIGQDGVETSFDQLRDVFIQPTHDKQNPLIYGVFTTLGSVFRGSAVCVYSLADVRAVFNGPFAHKEGHGYQMTAYSGKTPYPRPGACAGGFSVPRNRSSRNFGEDVLRFVRTHPLMYSSVYPVNRRPLLLLSDASYTYTSIAVDRVPAADGEYTVLFLGTDRGTVQKVMILPRGTKETESITLEEVEVFKVPSPIRNLRISSKRHQLYVSSDVGVTQLSLHRCGRYGDSCADCCLARDPYCAWDGKGCVRYTPSPSRRSRRQDVKHGDPLRQCRGYNTQVDRGIAERLQISVEGGSAYLQCDTHSPLESVTWLLQRDGSQHRREVRLSAIDGGAVLRSVQASDAGVYTCTGTENGFRRSRAKIRLNVLPRDLLEKLTAAPTLAPMAAQCPPPRRPRPRPSHTDRN

Target ORF information:

RefSeq Version	XM_012968257.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (sema3f), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968257.2

ORF Insert Sequence:

ATGGCTGGTG ACACCATGCT GGTATCGGCA TTACCTCCTC TGCTTCTGCT GCTTCTCAAC 
TTGCCCCCGT GTGTGCGAAC GACGCGGCCC CCTCGCGTGT ACCTTTCATA CAGAGATCTC 
ATGGAATCCC GCTCTGCGCG CTCCTTTACT TTTGGGTTTA ACACCAGCGA TTACCGTATT 
CTTCTCCTTG ATCAGGACCA GGACCGGATG TACGTGGGAA GCCGCGATTA TGTGCTGTCT 
CTTGACCTCG GAAACATCAA CCGGGAGCCC CTAATTATTC ATTGGCCAGC TCCACTGAAC 
CGTCACAAGG AATGCCTCAT GGCAGGGAGG GGGCGTGTAG ACGAATGCTA CAATTACCTG 
CGTGTCATTC AGCCACTGAA CCGTACCCAT CTCTATGCGT GTGGCACAGG AGCCTACCAG 
CCACAGTGTG CTGTTATCTA CAGGGGTTGG AGATCAGAGG ATTATGTGTT CCGCATTGTC 
CCTGCCTCGC TGGAGTCAGG AAAGGGAAGG TGCCCTTATG ATCCCAAGCA GGAAAACATG 
GCGACCTTAA TAGATGGCGT GTTGTATGCA GGGGTCCAGG TGGATTACAT GGGGACGGAC 
TCTGCCATTT TCCGGACAAT GGGGCACAAG CCCCCAATAC GCACGGAGCA GTACGACTCT 
CGATGGCTGA ATGATCCGTT ATTTGTGGGG TCCCTGGTGG TCTCAGAAAG CAGCTTCAAA 
GAGGATGACA AACTGTACAT CTTATTCCGG GAGAGAAGCC TAGAAGGGGA GAATGGCCCG 
GCGTTGGTGT CTCGTATTGC TCGTGTCTGC TTGAATGACG AGGGGGGTCT GCGCTCTCTG 
GTGGGAAAGT GGACTTCCTA TCTCAAGGCC CGGCTAGTGT GCTCCGTGAT TGGCCAGGAT 
GGTGTGGAGA CATCGTTTGA CCAACTCCGT GACGTCTTTA TCCAGCCGAC CCACGACAAA 
CAAAACCCGC TGATCTACGG AGTCTTCACT ACACTGGGTT CCGTGTTCCG GGGCTCCGCC 
GTCTGCGTTT ACTCTCTGGC TGATGTCCGT GCTGTGTTTA ATGGACCCTT TGCACACAAG 
GAGGGACATG GGTACCAGAT GACTGCCTAC TCCGGGAAAA CACCGTATCC CAGACCTGGA 
GCGTGCGCTG GCGGATTCTC CGTCCCCAGG AATCGATCGA GCAGGAACTT TGGGGAGGAT 
GTTTTGAGGT TTGTGCGCAC TCACCCCCTG ATGTACAGCT CCGTGTACCC CGTCAACCGC 
AGACCCCTAC TTTTGCTCTC AGATGCCTCC TACACCTACA CCAGCATTGC TGTGGACAGA 
GTGCCCGCGG CTGATGGGGA GTATACCGTC CTGTTCCTGG GCACAGACCG TGGTACTGTT 
CAGAAAGTAA TGATCCTGCC CCGGGGCACC AAGGAAACAG AAAGTATCAC CTTGGAGGAA 
GTAGAAGTGT TCAAGGTGCC ATCTCCTATC AGAAACCTCA GGATATCGTC AAAGAGGCAT 
CAGCTCTACG TCTCCTCAGA TGTCGGGGTC ACCCAGCTGT CTCTTCATCG TTGTGGTCGT 
TATGGAGATT CCTGCGCTGA TTGCTGTCTG GCCAGAGACC CATATTGTGC CTGGGATGGA 
AAGGGCTGTG TGCGTTACAC CCCTTCACCC AGCAGGAGGA GTCGGCGCCA GGATGTTAAA 
CATGGAGACC CCTTAAGGCA ATGCCGGGGA TACAATACAC AAGTGGATCG AGGCATTGCA 
GAGAGGCTGC AGATCAGCGT GGAAGGAGGG AGTGCATACC TTCAGTGCGA CACCCACTCC 
CCACTTGAGA GCGTCACCTG GTTACTGCAA AGAGATGGCT CCCAGCATCG GCGAGAGGTG 
CGACTCTCGG CCATTGATGG GGGCGCAGTG CTACGCTCAG TACAAGCCAG CGACGCGGGG 
GTCTATACCT GCACGGGTAC GGAGAACGGT TTCCGGCGAT CACGTGCAAA AATACGGCTC 
AATGTTCTGC CACGGGACCT ACTAGAAAAA CTAACAGCTG CGCCAACCCT TGCTCCAATG 
GCAGCACAAT GCCCACCGCC CCGCAGACCA AGACCGCGGC CATCTCACAC AGACCGTAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fl ( XM_012968258.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823712.1 Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (sema3f), transcript variant X2, mRNA.

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CloneID	OXa26788
Clone ID Related Accession (Same CDS sequence)	XM_012968258.2 , XM_012968258.3
Accession Version	XM_012968258.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968258.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTG ACACCATGCT GGTATCGGCA TTACCTCCTC TGCTTCTGCT GCTTCTCAAC 
TTGCCCCCGT GTGTGCGAAC GACGCGGCCC CCTCGCGTGT ACCTTTCATA CAGAGATCTC 
ATGGAATCCC GCTCTGCGCG CTCCTTTACT TTTGGGTTTA ACACCAGCGA TTACCGTATT 
CTTCTCCTTG ATCAGGACCA GGACCGGATG TACGTGGGAA GCCGCGATTA TGTGCTGTCT 
CTTGACCTCG GAAACATCAA CCGGGAGCCC CTAATTATTC ATTGGCCAGC TCCACTGAAC 
CGTCACAAGG AATGCCTCAT GGCAGGGAGG GGGCGTGTAG ACGAATGCTA CAATTACCTG 
CGTGTCATTC AGCCACTGAA CCGTACCCAT CTCTATGCGT GTGGCACAGG AGCCTACCAG 
CCACAGTGTG CTGTTATCTA CAGGGGTTGG AGATCAGAGG ATTATGTGTT CCGCATTGTC 
CCTGCCTCGC TGGAGTCAGG AAAGGGAAGG TGCCCTTATG ATCCCAAGCA GGAAAACATG 
GCGACCTTAA TAGATGGCGT GTTGTATGCA GGGGTCCAGG TGGATTACAT GGGGACGGAC 
TCTGCCATTT TCCGGACAAT GGGGCACAAG CCCCCAATAC GCACGGAGCA GTACGACTCT 
CGATGGCTGA ATGATCCGTT ATTTGTGGGG TCCCTGGTGG TCTCAGAAAG CAGCTTCAAA 
GAGGATGACA AACTGTACAT CTTATTCCGG GAGAGAAGCC TAGAAGGGGA GAATGGCCCG 
GCGTTGGTGT CTCGTATTGC TCGTGTCTGC TTGAATGACG AGGGGGGTCT GCGCTCTCTG 
GTGGGAAAGT GGACTTCCTA TCTCAAGGCC CGGCTAGTGT GCTCCGTGAT TGGCCAGGAT 
GGTGTGGAGA CATCGTTTGA CCAACTCCGT GACGTCTTTA TCCAGCCGAC CCACGACAAA 
CAAAACCCGC TGATCTACGG AGTCTTCACT ACACTGGGTT CCGTGTTCCG GGGCTCCGCC 
GTCTGCGTTT ACTCTCTGGC TGATGTCCGT GCTGTGTTTA ATGGACCCTT TGCACACAAG 
GAGGGACATG GGTACCAGAT GACTGCCTAC TCCGGGAAAA CACCGTATCC CAGACCTGGA 
GCGTGCGCTG GCGGATTCTC CGTCCCCAGG AATCGATCGA GCAGGAACTT TGGGGAGGAT 
GTTTTGAGGT TTGTGCGCAC TCACCCCCTG ATGTACAGCT CCGTGTACCC CGTCAACCGC 
AGACCCCTAC TTTTGCTCTC AGATGCCTCC TACACCTACA CCAGCATTGC TGTGGACAGA 
GTGCCCGCGG CTGATGGGGA GTATACCGTC CTGTTCCTGG GCACAGACCG TGGTACTGTT 
CAGAAAGTAA TGATCCTGCC CCGGGGCACC AAGGAAACAG AAAGTATCAC CTTGGAGGAA 
GTAGAAGTGT TCAAGGTGCC ATCTCCTATC AGAAACCTCA GGATATCGTC AAAGAGGCAT 
CAGCTCTACG TCTCCTCAGA TGTCGGGGTC ACCCAGCTGT CTCTTCATCG TTGTGGTCGT 
TATGGAGATT CCTGCGCTGA TTGCTGTCTG GCCAGAGACC CATATTGTGC CTGGGATGGA 
AAGGGCTGTG TGCGTTACAC CCCTTCACCC AGCAGGAGGA GTCGGCGCCA GGATGTTAAA 
CATGGAGACC CCTTAAGGCA ATGCCGGGGA TACAATACAC AAGATCGAGG CATTGCAGAG 
AGGCTGCAGA TCAGCGTGGA AGGAGGGAGT GCATACCTTC AGTGCGACAC CCACTCCCCA 
CTTGAGAGCG TCACCTGGTT ACTGCAAAGA GATGGCTCCC AGCATCGGCG AGAGGTGCGA 
CTCTCGGCCA TTGATGGGGG CGCAGTGCTA CGCTCAGTAC AAGCCAGCGA CGCGGGGGTC 
TATACCTGCA CGGGTACGGA GAACGGTTTC CGGCGATCAC GTGCAAAAAT ACGGCTCAAT 
GTTCTGCCAC GGGACCTACT AGAAAAACTA ACAGCTGCGC CAACCCTTGC TCCAATGGCA 
GCACAATGCC CACCGCCCCG CAGACCAAGA CCGCGGCCAT CTCACACAGA CCGTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823712.1
CDS	19..2118
Translation	MAGDTMLVSALPPLLLLLLNLPPCVRTTRPPRVYLSYRDLMESRSARSFTFGFNTSDYRILLLDQDQDRMYVGSRDYVLSLDLGNINREPLIIHWPAPLNRHKECLMAGRGRVDECYNYLRVIQPLNRTHLYACGTGAYQPQCAVIYRGWRSEDYVFRIVPASLESGKGRCPYDPKQENMATLIDGVLYAGVQVDYMGTDSAIFRTMGHKPPIRTEQYDSRWLNDPLFVGSLVVSESSFKEDDKLYILFRERSLEGENGPALVSRIARVCLNDEGGLRSLVGKWTSYLKARLVCSVIGQDGVETSFDQLRDVFIQPTHDKQNPLIYGVFTTLGSVFRGSAVCVYSLADVRAVFNGPFAHKEGHGYQMTAYSGKTPYPRPGACAGGFSVPRNRSSRNFGEDVLRFVRTHPLMYSSVYPVNRRPLLLLSDASYTYTSIAVDRVPAADGEYTVLFLGTDRGTVQKVMILPRGTKETESITLEEVEVFKVPSPIRNLRISSKRHQLYVSSDVGVTQLSLHRCGRYGDSCADCCLARDPYCAWDGKGCVRYTPSPSRRSRRQDVKHGDPLRQCRGYNTQDRGIAERLQISVEGGSAYLQCDTHSPLESVTWLLQRDGSQHRREVRLSAIDGGAVLRSVQASDAGVYTCTGTENGFRRSRAKIRLNVLPRDLLEKLTAAPTLAPMAAQCPPPRRPRPRPSHTDRN

Target ORF information:

RefSeq Version	XM_012968258.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F (sema3f), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968258.2

ORF Insert Sequence:

ATGGCTGGTG ACACCATGCT GGTATCGGCA TTACCTCCTC TGCTTCTGCT GCTTCTCAAC 
TTGCCCCCGT GTGTGCGAAC GACGCGGCCC CCTCGCGTGT ACCTTTCATA CAGAGATCTC 
ATGGAATCCC GCTCTGCGCG CTCCTTTACT TTTGGGTTTA ACACCAGCGA TTACCGTATT 
CTTCTCCTTG ATCAGGACCA GGACCGGATG TACGTGGGAA GCCGCGATTA TGTGCTGTCT 
CTTGACCTCG GAAACATCAA CCGGGAGCCC CTAATTATTC ATTGGCCAGC TCCACTGAAC 
CGTCACAAGG AATGCCTCAT GGCAGGGAGG GGGCGTGTAG ACGAATGCTA CAATTACCTG 
CGTGTCATTC AGCCACTGAA CCGTACCCAT CTCTATGCGT GTGGCACAGG AGCCTACCAG 
CCACAGTGTG CTGTTATCTA CAGGGGTTGG AGATCAGAGG ATTATGTGTT CCGCATTGTC 
CCTGCCTCGC TGGAGTCAGG AAAGGGAAGG TGCCCTTATG ATCCCAAGCA GGAAAACATG 
GCGACCTTAA TAGATGGCGT GTTGTATGCA GGGGTCCAGG TGGATTACAT GGGGACGGAC 
TCTGCCATTT TCCGGACAAT GGGGCACAAG CCCCCAATAC GCACGGAGCA GTACGACTCT 
CGATGGCTGA ATGATCCGTT ATTTGTGGGG TCCCTGGTGG TCTCAGAAAG CAGCTTCAAA 
GAGGATGACA AACTGTACAT CTTATTCCGG GAGAGAAGCC TAGAAGGGGA GAATGGCCCG 
GCGTTGGTGT CTCGTATTGC TCGTGTCTGC TTGAATGACG AGGGGGGTCT GCGCTCTCTG 
GTGGGAAAGT GGACTTCCTA TCTCAAGGCC CGGCTAGTGT GCTCCGTGAT TGGCCAGGAT 
GGTGTGGAGA CATCGTTTGA CCAACTCCGT GACGTCTTTA TCCAGCCGAC CCACGACAAA 
CAAAACCCGC TGATCTACGG AGTCTTCACT ACACTGGGTT CCGTGTTCCG GGGCTCCGCC 
GTCTGCGTTT ACTCTCTGGC TGATGTCCGT GCTGTGTTTA ATGGACCCTT TGCACACAAG 
GAGGGACATG GGTACCAGAT GACTGCCTAC TCCGGGAAAA CACCGTATCC CAGACCTGGA 
GCGTGCGCTG GCGGATTCTC CGTCCCCAGG AATCGATCGA GCAGGAACTT TGGGGAGGAT 
GTTTTGAGGT TTGTGCGCAC TCACCCCCTG ATGTACAGCT CCGTGTACCC CGTCAACCGC 
AGACCCCTAC TTTTGCTCTC AGATGCCTCC TACACCTACA CCAGCATTGC TGTGGACAGA 
GTGCCCGCGG CTGATGGGGA GTATACCGTC CTGTTCCTGG GCACAGACCG TGGTACTGTT 
CAGAAAGTAA TGATCCTGCC CCGGGGCACC AAGGAAACAG AAAGTATCAC CTTGGAGGAA 
GTAGAAGTGT TCAAGGTGCC ATCTCCTATC AGAAACCTCA GGATATCGTC AAAGAGGCAT 
CAGCTCTACG TCTCCTCAGA TGTCGGGGTC ACCCAGCTGT CTCTTCATCG TTGTGGTCGT 
TATGGAGATT CCTGCGCTGA TTGCTGTCTG GCCAGAGACC CATATTGTGC CTGGGATGGA 
AAGGGCTGTG TGCGTTACAC CCCTTCACCC AGCAGGAGGA GTCGGCGCCA GGATGTTAAA 
CATGGAGACC CCTTAAGGCA ATGCCGGGGA TACAATACAC AAGATCGAGG CATTGCAGAG 
AGGCTGCAGA TCAGCGTGGA AGGAGGGAGT GCATACCTTC AGTGCGACAC CCACTCCCCA 
CTTGAGAGCG TCACCTGGTT ACTGCAAAGA GATGGCTCCC AGCATCGGCG AGAGGTGCGA 
CTCTCGGCCA TTGATGGGGG CGCAGTGCTA CGCTCAGTAC AAGCCAGCGA CGCGGGGGTC 
TATACCTGCA CGGGTACGGA GAACGGTTTC CGGCGATCAC GTGCAAAAAT ACGGCTCAAT 
GTTCTGCCAC GGGACCTACT AGAAAAACTA ACAGCTGCGC CAACCCTTGC TCCAATGGCA 
GCACAATGCC CACCGCCCCG CAGACCAAGA CCGCGGCCAT CTCACACAGA CCGTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fl ( XM_012968259.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823713.1 Xenopus tropicalis semaphorin 3F like (sema3fl), transcript variant X3, mRNA.

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CloneID	OXa23710
Clone ID Related Accession (Same CDS sequence)	XM_012968259.2 , NM_001006923.1 , XM_012968259.3
Accession Version	XM_012968259.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968259.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTAT CGGCATTACC TCCTCTGCTT CTGCTGCTTC TCAACTTGCC CCCGTGTGTG 
CGAACGACGC GGCCCCCTCG CGTGTACCTT TCATACAGAG ATCTCATGGA ATCCCGCTCT 
GCGCGCTCCT TTACTTTTGG GTTTAACACC AGCGATTACC GTATTCTTCT CCTTGATCAG 
GACCAGGACC GGATGTACGT GGGAAGCCGC GATTATGTGC TGTCTCTTGA CCTCGGAAAC 
ATCAACCGGG AGCCCCTAAT TATTCATTGG CCAGCTCCAC TGAACCGTCA CAAGGAATGC 
CTCATGGCAG GGAGGGGGCG TGTAGACGAA TGCTACAATT ACCTGCGTGT CATTCAGCCA 
CTGAACCGTA CCCATCTCTA TGCGTGTGGC ACAGGAGCCT ACCAGCCACA GTGTGCTGTT 
ATCTACAGGG GTTGGAGATC AGAGGATTAT GTGTTCCGCA TTGTCCCTGC CTCGCTGGAG 
TCAGGAAAGG GAAGGTGCCC TTATGATCCC AAGCAGGAAA ACATGGCGAC CTTAATAGAT 
GGCGTGTTGT ATGCAGGGGT CCAGGTGGAT TACATGGGGA CGGACTCTGC CATTTTCCGG 
ACAATGGGGC ACAAGCCCCC AATACGCACG GAGCAGTACG ACTCTCGATG GCTGAATGAT 
CCGTTATTTG TGGGGTCCCT GGTGGTCTCA GAAAGCAGCT TCAAAGAGGA TGACAAACTG 
TACATCTTAT TCCGGGAGAG AAGCCTAGAA GGGGAGAATG GCCCGGCGTT GGTGTCTCGT 
ATTGCTCGTG TCTGCTTGAA TGACGAGGGG GGTCTGCGCT CTCTGGTGGG AAAGTGGACT 
TCCTATCTCA AGGCCCGGCT AGTGTGCTCC GTGATTGGCC AGGATGGTGT GGAGACATCG 
TTTGACCAAC TCCGTGACGT CTTTATCCAG CCGACCCACG ACAAACAAAA CCCGCTGATC 
TACGGAGTCT TCACTACACT GGGTTCCGTG TTCCGGGGCT CCGCCGTCTG CGTTTACTCT 
CTGGCTGATG TCCGTGCTGT GTTTAATGGA CCCTTTGCAC ACAAGGAGGG ACATGGGTAC 
CAGATGACTG CCTACTCCGG GAAAACACCG TATCCCAGAC CTGGAGCGTG CGCTGGCGGA 
TTCTCCGTCC CCAGGAATCG ATCGAGCAGG AACTTTGGGG AGGATGTTTT GAGGTTTGTG 
CGCACTCACC CCCTGATGTA CAGCTCCGTG TACCCCGTCA ACCGCAGACC CCTACTTTTG 
CTCTCAGATG CCTCCTACAC CTACACCAGC ATTGCTGTGG ACAGAGTGCC CGCGGCTGAT 
GGGGAGTATA CCGTCCTGTT CCTGGGCACA GACCGTGGTA CTGTTCAGAA AGTAATGATC 
CTGCCCCGGG GCACCAAGGA AACAGAAAGT ATCACCTTGG AGGAAGTAGA AGTGTTCAAG 
GTGCCATCTC CTATCAGAAA CCTCAGGATA TCGTCAAAGA GGCATCAGCT CTACGTCTCC 
TCAGATGTCG GGGTCACCCA GCTGTCTCTT CATCGTTGTG GTCGTTATGG AGATTCCTGC 
GCTGATTGCT GTCTGGCCAG AGACCCATAT TGTGCCTGGG ATGGAAAGGG CTGTGTGCGT 
TACACCCCTT CACCCAGCAG GAGGAGTCGG CGCCAGGATG TTAAACATGG AGACCCCTTA 
AGGCAATGCC GGGGATACAA TACACAAGTG GATCGAGGCA TTGCAGAGAG GCTGCAGATC 
AGCGTGGAAG GAGGGAGTGC ATACCTTCAG TGCGACACCC ACTCCCCACT TGAGAGCGTC 
ACCTGGTTAC TGCAAAGAGA TGGCTCCCAG CATCGGCGAG AGGTGCGACT CTCGGCCATT 
GATGGGGGCG CAGTGCTACG CTCAGTACAA GCCAGCGACG CGGGGGTCTA TACCTGCACG 
GGTACGGAGA ACGGTTTCCG GCGATCACGT GCAAAAATAC GGCTCAATGT TCTGCCACGG 
GACCTACTAG AAAAACTAAC AGCTGCGCCA ACCCTTGCTC CAATGGCAGC ACAATGCCCA 
CCGCCCCGCA GACCAAGACC GCGGCCATCT CACACAGACC GTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823713.1
CDS	120..2207
Translation	MLVSALPPLLLLLLNLPPCVRTTRPPRVYLSYRDLMESRSARSFTFGFNTSDYRILLLDQDQDRMYVGSRDYVLSLDLGNINREPLIIHWPAPLNRHKECLMAGRGRVDECYNYLRVIQPLNRTHLYACGTGAYQPQCAVIYRGWRSEDYVFRIVPASLESGKGRCPYDPKQENMATLIDGVLYAGVQVDYMGTDSAIFRTMGHKPPIRTEQYDSRWLNDPLFVGSLVVSESSFKEDDKLYILFRERSLEGENGPALVSRIARVCLNDEGGLRSLVGKWTSYLKARLVCSVIGQDGVETSFDQLRDVFIQPTHDKQNPLIYGVFTTLGSVFRGSAVCVYSLADVRAVFNGPFAHKEGHGYQMTAYSGKTPYPRPGACAGGFSVPRNRSSRNFGEDVLRFVRTHPLMYSSVYPVNRRPLLLLSDASYTYTSIAVDRVPAADGEYTVLFLGTDRGTVQKVMILPRGTKETESITLEEVEVFKVPSPIRNLRISSKRHQLYVSSDVGVTQLSLHRCGRYGDSCADCCLARDPYCAWDGKGCVRYTPSPSRRSRRQDVKHGDPLRQCRGYNTQVDRGIAERLQISVEGGSAYLQCDTHSPLESVTWLLQRDGSQHRREVRLSAIDGGAVLRSVQASDAGVYTCTGTENGFRRSRAKIRLNVLPRDLLEKLTAAPTLAPMAAQCPPPRRPRPRPSHTDRN

Target ORF information:

RefSeq Version	XM_012968259.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis semaphorin 3F like (sema3fl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968259.3

ORF Insert Sequence:

ATGCTGGTAT CGGCATTACC TCCTCTGCTT CTGCTGCTTC TCAACTTGCC CCCGTGTGTG 
CGAACGACGC GGCCCCCTCG CGTGTACCTT TCATACAGAG ATCTCATGGA ATCCCGCTCT 
GCGCGCTCCT TTACTTTTGG GTTTAACACC AGCGATTACC GTATTCTTCT CCTTGATCAG 
GACCAGGACC GGATGTACGT GGGAAGCCGC GATTATGTGC TGTCTCTTGA CCTCGGAAAC 
ATCAACCGGG AGCCCCTAAT TATTCATTGG CCAGCTCCAC TGAACCGTCA CAAGGAATGC 
CTCATGGCAG GGAGGGGGCG TGTAGACGAA TGCTACAATT ACCTGCGTGT CATTCAGCCA 
CTGAACCGTA CCCATCTCTA TGCGTGTGGC ACAGGAGCCT ACCAGCCACA GTGTGCTGTT 
ATCTACAGGG GTTGGAGATC AGAGGATTAT GTGTTCCGCA TTGTCCCTGC CTCGCTGGAG 
TCAGGAAAGG GAAGGTGCCC TTATGATCCC AAGCAGGAAA ACATGGCGAC CTTAATAGAT 
GGCGTGTTGT ATGCAGGGGT CCAGGTGGAT TACATGGGGA CGGACTCTGC CATTTTCCGG 
ACAATGGGGC ACAAGCCCCC AATACGCACG GAGCAGTACG ACTCTCGATG GCTGAATGAT 
CCGTTATTTG TGGGGTCCCT GGTGGTCTCA GAAAGCAGCT TCAAAGAGGA TGACAAACTG 
TACATCTTAT TCCGGGAGAG AAGCCTAGAA GGGGAGAATG GCCCGGCGTT GGTGTCTCGT 
ATTGCTCGTG TCTGCTTGAA TGACGAGGGG GGTCTGCGCT CTCTGGTGGG AAAGTGGACT 
TCCTATCTCA AGGCCCGGCT AGTGTGCTCC GTGATTGGCC AGGATGGTGT GGAGACATCG 
TTTGACCAAC TCCGTGACGT CTTTATCCAG CCGACCCACG ACAAACAAAA CCCGCTGATC 
TACGGAGTCT TCACTACACT GGGTTCCGTG TTCCGGGGCT CCGCCGTCTG CGTTTACTCT 
CTGGCTGATG TCCGTGCTGT GTTTAATGGA CCCTTTGCAC ACAAGGAGGG ACATGGGTAC 
CAGATGACTG CCTACTCCGG GAAAACACCG TATCCCAGAC CTGGAGCGTG CGCTGGCGGA 
TTCTCCGTCC CCAGGAATCG ATCGAGCAGG AACTTTGGGG AGGATGTTTT GAGGTTTGTG 
CGCACTCACC CCCTGATGTA CAGCTCCGTG TACCCCGTCA ACCGCAGACC CCTACTTTTG 
CTCTCAGATG CCTCCTACAC CTACACCAGC ATTGCTGTGG ACAGAGTGCC CGCGGCTGAT 
GGGGAGTATA CCGTCCTGTT CCTGGGCACA GACCGTGGTA CTGTTCAGAA AGTAATGATC 
CTGCCCCGGG GCACCAAGGA AACAGAAAGT ATCACCTTGG AGGAAGTAGA AGTGTTCAAG 
GTGCCATCTC CTATCAGAAA CCTCAGGATA TCGTCAAAGA GGCATCAGCT CTACGTCTCC 
TCAGATGTCG GGGTCACCCA GCTGTCTCTT CATCGTTGTG GTCGTTATGG AGATTCCTGC 
GCTGATTGCT GTCTGGCCAG AGACCCATAT TGTGCCTGGG ATGGAAAGGG CTGTGTGCGT 
TACACCCCTT CACCCAGCAG GAGGAGTCGG CGCCAGGATG TTAAACATGG AGACCCCTTA 
AGGCAATGCC GGGGATACAA TACACAAGTG GATCGAGGCA TTGCAGAGAG GCTGCAGATC 
AGCGTGGAAG GAGGGAGTGC ATACCTTCAG TGCGACACCC ACTCCCCACT TGAGAGCGTC 
ACCTGGTTAC TGCAAAGAGA TGGCTCCCAG CATCGGCGAG AGGTGCGACT CTCGGCCATT 
GATGGGGGCG CAGTGCTACG CTCAGTACAA GCCAGCGACG CGGGGGTCTA TACCTGCACG 
GGTACGGAGA ACGGTTTCCG GCGATCACGT GCAAAAATAC GGCTCAATGT TCTGCCACGG 
GACCTACTAG AAAAACTAAC AGCTGCGCCA ACCCTTGCTC CAATGGCAGC ACAATGCCCA 
CCGCCCCGCA GACCAAGACC GCGGCCATCT CACACAGACC GTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fl ( XM_012968257.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823711.1 Xenopus tropicalis semaphorin 3F like (sema3fl), transcript variant X1, mRNA.

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CloneID	OXa26787
Clone ID Related Accession (Same CDS sequence)	XM_012968257.2 , XM_012968257.3
Accession Version	XM_012968257.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTG ACACCATGCT GGTATCGGCA TTACCTCCTC TGCTTCTGCT GCTTCTCAAC 
TTGCCCCCGT GTGTGCGAAC GACGCGGCCC CCTCGCGTGT ACCTTTCATA CAGAGATCTC 
ATGGAATCCC GCTCTGCGCG CTCCTTTACT TTTGGGTTTA ACACCAGCGA TTACCGTATT 
CTTCTCCTTG ATCAGGACCA GGACCGGATG TACGTGGGAA GCCGCGATTA TGTGCTGTCT 
CTTGACCTCG GAAACATCAA CCGGGAGCCC CTAATTATTC ATTGGCCAGC TCCACTGAAC 
CGTCACAAGG AATGCCTCAT GGCAGGGAGG GGGCGTGTAG ACGAATGCTA CAATTACCTG 
CGTGTCATTC AGCCACTGAA CCGTACCCAT CTCTATGCGT GTGGCACAGG AGCCTACCAG 
CCACAGTGTG CTGTTATCTA CAGGGGTTGG AGATCAGAGG ATTATGTGTT CCGCATTGTC 
CCTGCCTCGC TGGAGTCAGG AAAGGGAAGG TGCCCTTATG ATCCCAAGCA GGAAAACATG 
GCGACCTTAA TAGATGGCGT GTTGTATGCA GGGGTCCAGG TGGATTACAT GGGGACGGAC 
TCTGCCATTT TCCGGACAAT GGGGCACAAG CCCCCAATAC GCACGGAGCA GTACGACTCT 
CGATGGCTGA ATGATCCGTT ATTTGTGGGG TCCCTGGTGG TCTCAGAAAG CAGCTTCAAA 
GAGGATGACA AACTGTACAT CTTATTCCGG GAGAGAAGCC TAGAAGGGGA GAATGGCCCG 
GCGTTGGTGT CTCGTATTGC TCGTGTCTGC TTGAATGACG AGGGGGGTCT GCGCTCTCTG 
GTGGGAAAGT GGACTTCCTA TCTCAAGGCC CGGCTAGTGT GCTCCGTGAT TGGCCAGGAT 
GGTGTGGAGA CATCGTTTGA CCAACTCCGT GACGTCTTTA TCCAGCCGAC CCACGACAAA 
CAAAACCCGC TGATCTACGG AGTCTTCACT ACACTGGGTT CCGTGTTCCG GGGCTCCGCC 
GTCTGCGTTT ACTCTCTGGC TGATGTCCGT GCTGTGTTTA ATGGACCCTT TGCACACAAG 
GAGGGACATG GGTACCAGAT GACTGCCTAC TCCGGGAAAA CACCGTATCC CAGACCTGGA 
GCGTGCGCTG GCGGATTCTC CGTCCCCAGG AATCGATCGA GCAGGAACTT TGGGGAGGAT 
GTTTTGAGGT TTGTGCGCAC TCACCCCCTG ATGTACAGCT CCGTGTACCC CGTCAACCGC 
AGACCCCTAC TTTTGCTCTC AGATGCCTCC TACACCTACA CCAGCATTGC TGTGGACAGA 
GTGCCCGCGG CTGATGGGGA GTATACCGTC CTGTTCCTGG GCACAGACCG TGGTACTGTT 
CAGAAAGTAA TGATCCTGCC CCGGGGCACC AAGGAAACAG AAAGTATCAC CTTGGAGGAA 
GTAGAAGTGT TCAAGGTGCC ATCTCCTATC AGAAACCTCA GGATATCGTC AAAGAGGCAT 
CAGCTCTACG TCTCCTCAGA TGTCGGGGTC ACCCAGCTGT CTCTTCATCG TTGTGGTCGT 
TATGGAGATT CCTGCGCTGA TTGCTGTCTG GCCAGAGACC CATATTGTGC CTGGGATGGA 
AAGGGCTGTG TGCGTTACAC CCCTTCACCC AGCAGGAGGA GTCGGCGCCA GGATGTTAAA 
CATGGAGACC CCTTAAGGCA ATGCCGGGGA TACAATACAC AAGTGGATCG AGGCATTGCA 
GAGAGGCTGC AGATCAGCGT GGAAGGAGGG AGTGCATACC TTCAGTGCGA CACCCACTCC 
CCACTTGAGA GCGTCACCTG GTTACTGCAA AGAGATGGCT CCCAGCATCG GCGAGAGGTG 
CGACTCTCGG CCATTGATGG GGGCGCAGTG CTACGCTCAG TACAAGCCAG CGACGCGGGG 
GTCTATACCT GCACGGGTAC GGAGAACGGT TTCCGGCGAT CACGTGCAAA AATACGGCTC 
AATGTTCTGC CACGGGACCT ACTAGAAAAA CTAACAGCTG CGCCAACCCT TGCTCCAATG 
GCAGCACAAT GCCCACCGCC CCGCAGACCA AGACCGCGGC CATCTCACAC AGACCGTAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823711.1
CDS	16..2118
Translation	MAGDTMLVSALPPLLLLLLNLPPCVRTTRPPRVYLSYRDLMESRSARSFTFGFNTSDYRILLLDQDQDRMYVGSRDYVLSLDLGNINREPLIIHWPAPLNRHKECLMAGRGRVDECYNYLRVIQPLNRTHLYACGTGAYQPQCAVIYRGWRSEDYVFRIVPASLESGKGRCPYDPKQENMATLIDGVLYAGVQVDYMGTDSAIFRTMGHKPPIRTEQYDSRWLNDPLFVGSLVVSESSFKEDDKLYILFRERSLEGENGPALVSRIARVCLNDEGGLRSLVGKWTSYLKARLVCSVIGQDGVETSFDQLRDVFIQPTHDKQNPLIYGVFTTLGSVFRGSAVCVYSLADVRAVFNGPFAHKEGHGYQMTAYSGKTPYPRPGACAGGFSVPRNRSSRNFGEDVLRFVRTHPLMYSSVYPVNRRPLLLLSDASYTYTSIAVDRVPAADGEYTVLFLGTDRGTVQKVMILPRGTKETESITLEEVEVFKVPSPIRNLRISSKRHQLYVSSDVGVTQLSLHRCGRYGDSCADCCLARDPYCAWDGKGCVRYTPSPSRRSRRQDVKHGDPLRQCRGYNTQVDRGIAERLQISVEGGSAYLQCDTHSPLESVTWLLQRDGSQHRREVRLSAIDGGAVLRSVQASDAGVYTCTGTENGFRRSRAKIRLNVLPRDLLEKLTAAPTLAPMAAQCPPPRRPRPRPSHTDRN

Target ORF information:

RefSeq Version	XM_012968257.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis semaphorin 3F like (sema3fl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968257.3

ORF Insert Sequence:

ATGGCTGGTG ACACCATGCT GGTATCGGCA TTACCTCCTC TGCTTCTGCT GCTTCTCAAC 
TTGCCCCCGT GTGTGCGAAC GACGCGGCCC CCTCGCGTGT ACCTTTCATA CAGAGATCTC 
ATGGAATCCC GCTCTGCGCG CTCCTTTACT TTTGGGTTTA ACACCAGCGA TTACCGTATT 
CTTCTCCTTG ATCAGGACCA GGACCGGATG TACGTGGGAA GCCGCGATTA TGTGCTGTCT 
CTTGACCTCG GAAACATCAA CCGGGAGCCC CTAATTATTC ATTGGCCAGC TCCACTGAAC 
CGTCACAAGG AATGCCTCAT GGCAGGGAGG GGGCGTGTAG ACGAATGCTA CAATTACCTG 
CGTGTCATTC AGCCACTGAA CCGTACCCAT CTCTATGCGT GTGGCACAGG AGCCTACCAG 
CCACAGTGTG CTGTTATCTA CAGGGGTTGG AGATCAGAGG ATTATGTGTT CCGCATTGTC 
CCTGCCTCGC TGGAGTCAGG AAAGGGAAGG TGCCCTTATG ATCCCAAGCA GGAAAACATG 
GCGACCTTAA TAGATGGCGT GTTGTATGCA GGGGTCCAGG TGGATTACAT GGGGACGGAC 
TCTGCCATTT TCCGGACAAT GGGGCACAAG CCCCCAATAC GCACGGAGCA GTACGACTCT 
CGATGGCTGA ATGATCCGTT ATTTGTGGGG TCCCTGGTGG TCTCAGAAAG CAGCTTCAAA 
GAGGATGACA AACTGTACAT CTTATTCCGG GAGAGAAGCC TAGAAGGGGA GAATGGCCCG 
GCGTTGGTGT CTCGTATTGC TCGTGTCTGC TTGAATGACG AGGGGGGTCT GCGCTCTCTG 
GTGGGAAAGT GGACTTCCTA TCTCAAGGCC CGGCTAGTGT GCTCCGTGAT TGGCCAGGAT 
GGTGTGGAGA CATCGTTTGA CCAACTCCGT GACGTCTTTA TCCAGCCGAC CCACGACAAA 
CAAAACCCGC TGATCTACGG AGTCTTCACT ACACTGGGTT CCGTGTTCCG GGGCTCCGCC 
GTCTGCGTTT ACTCTCTGGC TGATGTCCGT GCTGTGTTTA ATGGACCCTT TGCACACAAG 
GAGGGACATG GGTACCAGAT GACTGCCTAC TCCGGGAAAA CACCGTATCC CAGACCTGGA 
GCGTGCGCTG GCGGATTCTC CGTCCCCAGG AATCGATCGA GCAGGAACTT TGGGGAGGAT 
GTTTTGAGGT TTGTGCGCAC TCACCCCCTG ATGTACAGCT CCGTGTACCC CGTCAACCGC 
AGACCCCTAC TTTTGCTCTC AGATGCCTCC TACACCTACA CCAGCATTGC TGTGGACAGA 
GTGCCCGCGG CTGATGGGGA GTATACCGTC CTGTTCCTGG GCACAGACCG TGGTACTGTT 
CAGAAAGTAA TGATCCTGCC CCGGGGCACC AAGGAAACAG AAAGTATCAC CTTGGAGGAA 
GTAGAAGTGT TCAAGGTGCC ATCTCCTATC AGAAACCTCA GGATATCGTC AAAGAGGCAT 
CAGCTCTACG TCTCCTCAGA TGTCGGGGTC ACCCAGCTGT CTCTTCATCG TTGTGGTCGT 
TATGGAGATT CCTGCGCTGA TTGCTGTCTG GCCAGAGACC CATATTGTGC CTGGGATGGA 
AAGGGCTGTG TGCGTTACAC CCCTTCACCC AGCAGGAGGA GTCGGCGCCA GGATGTTAAA 
CATGGAGACC CCTTAAGGCA ATGCCGGGGA TACAATACAC AAGTGGATCG AGGCATTGCA 
GAGAGGCTGC AGATCAGCGT GGAAGGAGGG AGTGCATACC TTCAGTGCGA CACCCACTCC 
CCACTTGAGA GCGTCACCTG GTTACTGCAA AGAGATGGCT CCCAGCATCG GCGAGAGGTG 
CGACTCTCGG CCATTGATGG GGGCGCAGTG CTACGCTCAG TACAAGCCAG CGACGCGGGG 
GTCTATACCT GCACGGGTAC GGAGAACGGT TTCCGGCGAT CACGTGCAAA AATACGGCTC 
AATGTTCTGC CACGGGACCT ACTAGAAAAA CTAACAGCTG CGCCAACCCT TGCTCCAATG 
GCAGCACAAT GCCCACCGCC CCGCAGACCA AGACCGCGGC CATCTCACAC AGACCGTAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3fl ( XM_012968258.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823712.1 Xenopus tropicalis semaphorin 3F like (sema3fl), transcript variant X2, mRNA.

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CloneID	OXa26788
Clone ID Related Accession (Same CDS sequence)	XM_012968258.2 , XM_012968258.3
Accession Version	XM_012968258.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGTG ACACCATGCT GGTATCGGCA TTACCTCCTC TGCTTCTGCT GCTTCTCAAC 
TTGCCCCCGT GTGTGCGAAC GACGCGGCCC CCTCGCGTGT ACCTTTCATA CAGAGATCTC 
ATGGAATCCC GCTCTGCGCG CTCCTTTACT TTTGGGTTTA ACACCAGCGA TTACCGTATT 
CTTCTCCTTG ATCAGGACCA GGACCGGATG TACGTGGGAA GCCGCGATTA TGTGCTGTCT 
CTTGACCTCG GAAACATCAA CCGGGAGCCC CTAATTATTC ATTGGCCAGC TCCACTGAAC 
CGTCACAAGG AATGCCTCAT GGCAGGGAGG GGGCGTGTAG ACGAATGCTA CAATTACCTG 
CGTGTCATTC AGCCACTGAA CCGTACCCAT CTCTATGCGT GTGGCACAGG AGCCTACCAG 
CCACAGTGTG CTGTTATCTA CAGGGGTTGG AGATCAGAGG ATTATGTGTT CCGCATTGTC 
CCTGCCTCGC TGGAGTCAGG AAAGGGAAGG TGCCCTTATG ATCCCAAGCA GGAAAACATG 
GCGACCTTAA TAGATGGCGT GTTGTATGCA GGGGTCCAGG TGGATTACAT GGGGACGGAC 
TCTGCCATTT TCCGGACAAT GGGGCACAAG CCCCCAATAC GCACGGAGCA GTACGACTCT 
CGATGGCTGA ATGATCCGTT ATTTGTGGGG TCCCTGGTGG TCTCAGAAAG CAGCTTCAAA 
GAGGATGACA AACTGTACAT CTTATTCCGG GAGAGAAGCC TAGAAGGGGA GAATGGCCCG 
GCGTTGGTGT CTCGTATTGC TCGTGTCTGC TTGAATGACG AGGGGGGTCT GCGCTCTCTG 
GTGGGAAAGT GGACTTCCTA TCTCAAGGCC CGGCTAGTGT GCTCCGTGAT TGGCCAGGAT 
GGTGTGGAGA CATCGTTTGA CCAACTCCGT GACGTCTTTA TCCAGCCGAC CCACGACAAA 
CAAAACCCGC TGATCTACGG AGTCTTCACT ACACTGGGTT CCGTGTTCCG GGGCTCCGCC 
GTCTGCGTTT ACTCTCTGGC TGATGTCCGT GCTGTGTTTA ATGGACCCTT TGCACACAAG 
GAGGGACATG GGTACCAGAT GACTGCCTAC TCCGGGAAAA CACCGTATCC CAGACCTGGA 
GCGTGCGCTG GCGGATTCTC CGTCCCCAGG AATCGATCGA GCAGGAACTT TGGGGAGGAT 
GTTTTGAGGT TTGTGCGCAC TCACCCCCTG ATGTACAGCT CCGTGTACCC CGTCAACCGC 
AGACCCCTAC TTTTGCTCTC AGATGCCTCC TACACCTACA CCAGCATTGC TGTGGACAGA 
GTGCCCGCGG CTGATGGGGA GTATACCGTC CTGTTCCTGG GCACAGACCG TGGTACTGTT 
CAGAAAGTAA TGATCCTGCC CCGGGGCACC AAGGAAACAG AAAGTATCAC CTTGGAGGAA 
GTAGAAGTGT TCAAGGTGCC ATCTCCTATC AGAAACCTCA GGATATCGTC AAAGAGGCAT 
CAGCTCTACG TCTCCTCAGA TGTCGGGGTC ACCCAGCTGT CTCTTCATCG TTGTGGTCGT 
TATGGAGATT CCTGCGCTGA TTGCTGTCTG GCCAGAGACC CATATTGTGC CTGGGATGGA 
AAGGGCTGTG TGCGTTACAC CCCTTCACCC AGCAGGAGGA GTCGGCGCCA GGATGTTAAA 
CATGGAGACC CCTTAAGGCA ATGCCGGGGA TACAATACAC AAGATCGAGG CATTGCAGAG 
AGGCTGCAGA TCAGCGTGGA AGGAGGGAGT GCATACCTTC AGTGCGACAC CCACTCCCCA 
CTTGAGAGCG TCACCTGGTT ACTGCAAAGA GATGGCTCCC AGCATCGGCG AGAGGTGCGA 
CTCTCGGCCA TTGATGGGGG CGCAGTGCTA CGCTCAGTAC AAGCCAGCGA CGCGGGGGTC 
TATACCTGCA CGGGTACGGA GAACGGTTTC CGGCGATCAC GTGCAAAAAT ACGGCTCAAT 
GTTCTGCCAC GGGACCTACT AGAAAAACTA ACAGCTGCGC CAACCCTTGC TCCAATGGCA 
GCACAATGCC CACCGCCCCG CAGACCAAGA CCGCGGCCAT CTCACACAGA CCGTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823712.1
CDS	17..2116
Translation	MAGDTMLVSALPPLLLLLLNLPPCVRTTRPPRVYLSYRDLMESRSARSFTFGFNTSDYRILLLDQDQDRMYVGSRDYVLSLDLGNINREPLIIHWPAPLNRHKECLMAGRGRVDECYNYLRVIQPLNRTHLYACGTGAYQPQCAVIYRGWRSEDYVFRIVPASLESGKGRCPYDPKQENMATLIDGVLYAGVQVDYMGTDSAIFRTMGHKPPIRTEQYDSRWLNDPLFVGSLVVSESSFKEDDKLYILFRERSLEGENGPALVSRIARVCLNDEGGLRSLVGKWTSYLKARLVCSVIGQDGVETSFDQLRDVFIQPTHDKQNPLIYGVFTTLGSVFRGSAVCVYSLADVRAVFNGPFAHKEGHGYQMTAYSGKTPYPRPGACAGGFSVPRNRSSRNFGEDVLRFVRTHPLMYSSVYPVNRRPLLLLSDASYTYTSIAVDRVPAADGEYTVLFLGTDRGTVQKVMILPRGTKETESITLEEVEVFKVPSPIRNLRISSKRHQLYVSSDVGVTQLSLHRCGRYGDSCADCCLARDPYCAWDGKGCVRYTPSPSRRSRRQDVKHGDPLRQCRGYNTQDRGIAERLQISVEGGSAYLQCDTHSPLESVTWLLQRDGSQHRREVRLSAIDGGAVLRSVQASDAGVYTCTGTENGFRRSRAKIRLNVLPRDLLEKLTAAPTLAPMAAQCPPPRRPRPRPSHTDRN

Target ORF information:

RefSeq Version	XM_012968258.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis semaphorin 3F like (sema3fl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968258.3

ORF Insert Sequence:

ATGGCTGGTG ACACCATGCT GGTATCGGCA TTACCTCCTC TGCTTCTGCT GCTTCTCAAC 
TTGCCCCCGT GTGTGCGAAC GACGCGGCCC CCTCGCGTGT ACCTTTCATA CAGAGATCTC 
ATGGAATCCC GCTCTGCGCG CTCCTTTACT TTTGGGTTTA ACACCAGCGA TTACCGTATT 
CTTCTCCTTG ATCAGGACCA GGACCGGATG TACGTGGGAA GCCGCGATTA TGTGCTGTCT 
CTTGACCTCG GAAACATCAA CCGGGAGCCC CTAATTATTC ATTGGCCAGC TCCACTGAAC 
CGTCACAAGG AATGCCTCAT GGCAGGGAGG GGGCGTGTAG ACGAATGCTA CAATTACCTG 
CGTGTCATTC AGCCACTGAA CCGTACCCAT CTCTATGCGT GTGGCACAGG AGCCTACCAG 
CCACAGTGTG CTGTTATCTA CAGGGGTTGG AGATCAGAGG ATTATGTGTT CCGCATTGTC 
CCTGCCTCGC TGGAGTCAGG AAAGGGAAGG TGCCCTTATG ATCCCAAGCA GGAAAACATG 
GCGACCTTAA TAGATGGCGT GTTGTATGCA GGGGTCCAGG TGGATTACAT GGGGACGGAC 
TCTGCCATTT TCCGGACAAT GGGGCACAAG CCCCCAATAC GCACGGAGCA GTACGACTCT 
CGATGGCTGA ATGATCCGTT ATTTGTGGGG TCCCTGGTGG TCTCAGAAAG CAGCTTCAAA 
GAGGATGACA AACTGTACAT CTTATTCCGG GAGAGAAGCC TAGAAGGGGA GAATGGCCCG 
GCGTTGGTGT CTCGTATTGC TCGTGTCTGC TTGAATGACG AGGGGGGTCT GCGCTCTCTG 
GTGGGAAAGT GGACTTCCTA TCTCAAGGCC CGGCTAGTGT GCTCCGTGAT TGGCCAGGAT 
GGTGTGGAGA CATCGTTTGA CCAACTCCGT GACGTCTTTA TCCAGCCGAC CCACGACAAA 
CAAAACCCGC TGATCTACGG AGTCTTCACT ACACTGGGTT CCGTGTTCCG GGGCTCCGCC 
GTCTGCGTTT ACTCTCTGGC TGATGTCCGT GCTGTGTTTA ATGGACCCTT TGCACACAAG 
GAGGGACATG GGTACCAGAT GACTGCCTAC TCCGGGAAAA CACCGTATCC CAGACCTGGA 
GCGTGCGCTG GCGGATTCTC CGTCCCCAGG AATCGATCGA GCAGGAACTT TGGGGAGGAT 
GTTTTGAGGT TTGTGCGCAC TCACCCCCTG ATGTACAGCT CCGTGTACCC CGTCAACCGC 
AGACCCCTAC TTTTGCTCTC AGATGCCTCC TACACCTACA CCAGCATTGC TGTGGACAGA 
GTGCCCGCGG CTGATGGGGA GTATACCGTC CTGTTCCTGG GCACAGACCG TGGTACTGTT 
CAGAAAGTAA TGATCCTGCC CCGGGGCACC AAGGAAACAG AAAGTATCAC CTTGGAGGAA 
GTAGAAGTGT TCAAGGTGCC ATCTCCTATC AGAAACCTCA GGATATCGTC AAAGAGGCAT 
CAGCTCTACG TCTCCTCAGA TGTCGGGGTC ACCCAGCTGT CTCTTCATCG TTGTGGTCGT 
TATGGAGATT CCTGCGCTGA TTGCTGTCTG GCCAGAGACC CATATTGTGC CTGGGATGGA 
AAGGGCTGTG TGCGTTACAC CCCTTCACCC AGCAGGAGGA GTCGGCGCCA GGATGTTAAA 
CATGGAGACC CCTTAAGGCA ATGCCGGGGA TACAATACAC AAGATCGAGG CATTGCAGAG 
AGGCTGCAGA TCAGCGTGGA AGGAGGGAGT GCATACCTTC AGTGCGACAC CCACTCCCCA 
CTTGAGAGCG TCACCTGGTT ACTGCAAAGA GATGGCTCCC AGCATCGGCG AGAGGTGCGA 
CTCTCGGCCA TTGATGGGGG CGCAGTGCTA CGCTCAGTAC AAGCCAGCGA CGCGGGGGTC 
TATACCTGCA CGGGTACGGA GAACGGTTTC CGGCGATCAC GTGCAAAAAT ACGGCTCAAT 
GTTCTGCCAC GGGACCTACT AGAAAAACTA ACAGCTGCGC CAACCCTTGC TCCAATGGCA 
GCACAATGCC CACCGCCCCG CAGACCAAGA CCGCGGCCAT CTCACACAGA CCGTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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sema3fl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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