mmp23b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following mmp23b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mmp23b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa13179 XM_004916150.4
Latest version!
Xenopus tropicalis matrix metallopeptidase 23B (mmp23b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OXa62601 XM_031906131.1
Latest version!
Xenopus tropicalis matrix metallopeptidase 23B (mmp23b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OXa13179 XM_004916150.3 Xenopus tropicalis matrix metallopeptidase 23B (mmp23b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa13179
    Clone ID Related Accession (Same CDS sequence) XM_004916150.4 , XM_004916150.3
    Accession Version XM_004916150.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product matrix metalloproteinase-23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916150.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGTGACA TCCAGGAAAT TGAGCATTGG CAGAAAAGGT ACATCTGGAC ATTTCTTGCC 
    ATTTTTGCTG GAACAGTGCT GGTAGCTGGA ATTTTCACAG CATCTAATTC AGTGTCCTTG
    GACTCACAGG TAGACTTTGT TGTGGCTCCA ACCCCGGCTC TTCAACTGCC CCTTCAGCTC
    CCTCGCCACC TGCGGAACAA GCGTTACACA TTGACTCCAG GTCTTCTGAA ATGGGATCAT
    TATAACCTGA CATACAGTAT TGTTTCATTT CCAAGGAATT TGATCAATGA AAGTGACACA
    AAGAAGGGGA TGGCCCAGGC TTTCCAGATG TGGAGTGAAG TGTCTCCTTT TCACTTCAAG
    GAAGTCCCTG CAGACCAACC AAGTGACCTA GTAATAGGTT TCTATGGTAT CAATCACACA
    GACTGCTTGG AGTCCTATAT CCATTATTGC TTTGATGGAA CAACGGGCGA GTTGGCACAT
    GCGTACTTCC CTAAAACAGG AGAAATACAT TTTGATGACA GTGAATTTTG GATTTTGGGT
    AACACGAGGT TCAGTTGGAA GAAAGGGGTG TGGCTCACAG ACTTGGTTCA CGTGGCAGCC
    CATGAGATTG GCCATGCGTT GGGACTGATG CATTCCCTCA ATTCTAATGC TTTGATGCAC
    ATCAATGCAA CTCTGACCGG AAAAAAACTG ATTTCGCAGG ATGATATATG GGGAATCTAC
    AGTCTTTATG GTTGCAAGGA TAAATATTTA GTGTGTGCCT CCTGGGCCCA CAAGGGTTAC
    TGTGACTCTA GAAAGAGAAT TATGAAGAAG TATTGCCCTT ACAGCTGTGA CTTCTGCTAT
    GACTTCCCAT TTCCAACCAA ACTGCCTACA CCTCCACCTC CTAGAACGAA AGTCCGATTG
    GTTCCTGAAG GCAGAAATGT TACTTTACGG TGTGGGAAAA AAATTATGCA TAAAAAAGGA
    AAAGTTTTCT GGTACAAAGA CAAAGAGTTG CTGGAGTACT CTTACTATGG TTACCTTAGT
    CTCGATGATG ATCACATGAG TATAATTGCC AATGCCATAA ATGAGGGACT GTACACCTGC
    ATTGTGAAGA AGAGGGACCG CATTCTCACA ACTTACTCAT GGAACATACG ACTGCAAATG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916207.1
    CDS482..1624
    Translation

    Target ORF information:

    RefSeq Version XM_004916150.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis matrix metallopeptidase 23B (mmp23b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916150.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGTGACA TCCAGGAAAT TGAGCATTGG CAGAAAAGGT ACATCTGGAC ATTTCTTGCC 
    ATTTTTGCTG GAACAGTGCT GGTAGCTGGA ATTTTCACAG CATCTAATTC AGTGTCCTTG
    GACTCACAGG TAGACTTTGT TGTGGCTCCA ACCCCGGCTC TTCAACTGCC CCTTCAGCTC
    CCTCGCCACC TGCGGAACAA GCGTTACACA TTGACTCCAG GTCTTCTGAA ATGGGATCAT
    TATAACCTGA CATACAGTAT TGTTTCATTT CCAAGGAATT TGATCAATGA AAGTGACACA
    AAGAAGGGGA TGGCCCAGGC TTTCCAGATG TGGAGTGAAG TGTCTCCTTT TCACTTCAAG
    GAAGTCCCTG CAGACCAACC AAGTGACCTA GTAATAGGTT TCTATGGTAT CAATCACACA
    GACTGCTTGG AGTCCTATAT CCATTATTGC TTTGATGGAA CAACGGGCGA GTTGGCACAT
    GCGTACTTCC CTAAAACAGG AGAAATACAT TTTGATGACA GTGAATTTTG GATTTTGGGT
    AACACGAGGT TCAGTTGGAA GAAAGGGGTG TGGCTCACAG ACTTGGTTCA CGTGGCAGCC
    CATGAGATTG GCCATGCGTT GGGACTGATG CATTCCCTCA ATTCTAATGC TTTGATGCAC
    ATCAATGCAA CTCTGACCGG AAAAAAACTG ATTTCGCAGG ATGATATATG GGGAATCTAC
    AGTCTTTATG GTTGCAAGGA TAAATATTTA GTGTGTGCCT CCTGGGCCCA CAAGGGTTAC
    TGTGACTCTA GAAAGAGAAT TATGAAGAAG TATTGCCCTT ACAGCTGTGA CTTCTGCTAT
    GACTTCCCAT TTCCAACCAA ACTGCCTACA CCTCCACCTC CTAGAACGAA AGTCCGATTG
    GTTCCTGAAG GCAGAAATGT TACTTTACGG TGTGGGAAAA AAATTATGCA TAAAAAAGGA
    AAAGTTTTCT GGTACAAAGA CAAAGAGTTG CTGGAGTACT CTTACTATGG TTACCTTAGT
    CTCGATGATG ATCACATGAG TATAATTGCC AATGCCATAA ATGAGGGACT GTACACCTGC
    ATTGTGAAGA AGAGGGACCG CATTCTCACA ACTTACTCAT GGAACATACG ACTGCAAATG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa13179
    Clone ID Related Accession (Same CDS sequence) XM_004916150.4 , XM_004916150.3
    Accession Version XM_004916150.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product matrix metalloproteinase-23 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916150.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGTGACA TCCAGGAAAT TGAGCATTGG CAGAAAAGGT ACATCTGGAC ATTTCTTGCC 
    ATTTTTGCTG GAACAGTGCT GGTAGCTGGA ATTTTCACAG CATCTAATTC AGTGTCCTTG
    GACTCACAGG TAGACTTTGT TGTGGCTCCA ACCCCGGCTC TTCAACTGCC CCTTCAGCTC
    CCTCGCCACC TGCGGAACAA GCGTTACACA TTGACTCCAG GTCTTCTGAA ATGGGATCAT
    TATAACCTGA CATACAGTAT TGTTTCATTT CCAAGGAATT TGATCAATGA AAGTGACACA
    AAGAAGGGGA TGGCCCAGGC TTTCCAGATG TGGAGTGAAG TGTCTCCTTT TCACTTCAAG
    GAAGTCCCTG CAGACCAACC AAGTGACCTA GTAATAGGTT TCTATGGTAT CAATCACACA
    GACTGCTTGG AGTCCTATAT CCATTATTGC TTTGATGGAA CAACGGGCGA GTTGGCACAT
    GCGTACTTCC CTAAAACAGG AGAAATACAT TTTGATGACA GTGAATTTTG GATTTTGGGT
    AACACGAGGT TCAGTTGGAA GAAAGGGGTG TGGCTCACAG ACTTGGTTCA CGTGGCAGCC
    CATGAGATTG GCCATGCGTT GGGACTGATG CATTCCCTCA ATTCTAATGC TTTGATGCAC
    ATCAATGCAA CTCTGACCGG AAAAAAACTG ATTTCGCAGG ATGATATATG GGGAATCTAC
    AGTCTTTATG GTTGCAAGGA TAAATATTTA GTGTGTGCCT CCTGGGCCCA CAAGGGTTAC
    TGTGACTCTA GAAAGAGAAT TATGAAGAAG TATTGCCCTT ACAGCTGTGA CTTCTGCTAT
    GACTTCCCAT TTCCAACCAA ACTGCCTACA CCTCCACCTC CTAGAACGAA AGTCCGATTG
    GTTCCTGAAG GCAGAAATGT TACTTTACGG TGTGGGAAAA AAATTATGCA TAAAAAAGGA
    AAAGTTTTCT GGTACAAAGA CAAAGAGTTG CTGGAGTACT CTTACTATGG TTACCTTAGT
    CTCGATGATG ATCACATGAG TATAATTGCC AATGCCATAA ATGAGGGACT GTACACCTGC
    ATTGTGAAGA AGAGGGACCG CATTCTCACA ACTTACTCAT GGAACATACG ACTGCAAATG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916207.1
    CDS584..1726
    Translation

    Target ORF information:

    RefSeq Version XM_004916150.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis matrix metallopeptidase 23B (mmp23b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916150.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGTGACA TCCAGGAAAT TGAGCATTGG CAGAAAAGGT ACATCTGGAC ATTTCTTGCC 
    ATTTTTGCTG GAACAGTGCT GGTAGCTGGA ATTTTCACAG CATCTAATTC AGTGTCCTTG
    GACTCACAGG TAGACTTTGT TGTGGCTCCA ACCCCGGCTC TTCAACTGCC CCTTCAGCTC
    CCTCGCCACC TGCGGAACAA GCGTTACACA TTGACTCCAG GTCTTCTGAA ATGGGATCAT
    TATAACCTGA CATACAGTAT TGTTTCATTT CCAAGGAATT TGATCAATGA AAGTGACACA
    AAGAAGGGGA TGGCCCAGGC TTTCCAGATG TGGAGTGAAG TGTCTCCTTT TCACTTCAAG
    GAAGTCCCTG CAGACCAACC AAGTGACCTA GTAATAGGTT TCTATGGTAT CAATCACACA
    GACTGCTTGG AGTCCTATAT CCATTATTGC TTTGATGGAA CAACGGGCGA GTTGGCACAT
    GCGTACTTCC CTAAAACAGG AGAAATACAT TTTGATGACA GTGAATTTTG GATTTTGGGT
    AACACGAGGT TCAGTTGGAA GAAAGGGGTG TGGCTCACAG ACTTGGTTCA CGTGGCAGCC
    CATGAGATTG GCCATGCGTT GGGACTGATG CATTCCCTCA ATTCTAATGC TTTGATGCAC
    ATCAATGCAA CTCTGACCGG AAAAAAACTG ATTTCGCAGG ATGATATATG GGGAATCTAC
    AGTCTTTATG GTTGCAAGGA TAAATATTTA GTGTGTGCCT CCTGGGCCCA CAAGGGTTAC
    TGTGACTCTA GAAAGAGAAT TATGAAGAAG TATTGCCCTT ACAGCTGTGA CTTCTGCTAT
    GACTTCCCAT TTCCAACCAA ACTGCCTACA CCTCCACCTC CTAGAACGAA AGTCCGATTG
    GTTCCTGAAG GCAGAAATGT TACTTTACGG TGTGGGAAAA AAATTATGCA TAAAAAAGGA
    AAAGTTTTCT GGTACAAAGA CAAAGAGTTG CTGGAGTACT CTTACTATGG TTACCTTAGT
    CTCGATGATG ATCACATGAG TATAATTGCC AATGCCATAA ATGAGGGACT GTACACCTGC
    ATTGTGAAGA AGAGGGACCG CATTCTCACA ACTTACTCAT GGAACATACG ACTGCAAATG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62601
    Clone ID Related Accession (Same CDS sequence) XM_031906131.1
    Accession Version XM_031906131.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product matrix metalloproteinase-23 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGGTGACA TCCAGGAAAT TGAGCATTGG CAGAAAAGGT ACATCTGGAC ATTTCTTGCC 
    ATTTTTGCTG GAACAGTGCT GGTAGCTGGA ATTTTCACAG CATCTAATTC AGTGTCCTTG
    GACTCACAGG TAGACTTTGT TGTGGCTCCA ACCCCGGCTC TTCAACTGCC CCTTCAGCTC
    CCTCGCCACC TGCGGAACAA GCGTTACACA TTGACTCCAG GTCTTCTGAA ATGGGATCAT
    TATAACCTGA CATACAGGAA TTTGATCAAT GAAAGTGACA CAAAGAAGGG GATGGCCCAG
    GCTTTCCAGA TGTGGAGTGA AGTGTCTCCT TTTCACTTCA AGGAAGTCCC TGCAGACCAA
    CCAAGTGACC TAGTAATAGG TTTCTATGGT ATCAATCACA CAGACTGCTT GGAGTCCTAT
    ATCCATTATT GCTTTGATGG AACAACGGGC GAGTTGGCAC ATGCGTACTT CCCTAAAACA
    GGAGAAATAC ATTTTGATGA CAGTGAATTT TGGATTTTGG GTAACACGAG GTTCAGTTGG
    AAGAAAGGGG TGTGGCTCAC AGACTTGGTT CACGTGGCAG CCCATGAGAT TGGCCATGCG
    TTGGGACTGA TGCATTCCCT CAATTCTAAT GCTTTGATGC ACATCAATGC AACTCTGACC
    GGAAAAAAAC TGATTTCGCA GGATGATATA TGGGGAATCT ACAGTCTTTA TGGTTGCAAG
    GATAAATATT TAGTGTGTGC CTCCTGGGCC CACAAGGGTT ACTGTGACTC TAGAAAGAGA
    ATTATGAAGA AGTATTGCCC TTACAGCTGT GACTTCTGCT ATGACTTCCC ATTTCCAACC
    AAACTGCCTA CACCTCCACC TCCTAGAACG AAAGTCCGAT TGGTTCCTGA AGGCAGAAAT
    GTTACTTTAC GGTGTGGGAA AAAAATTATG CATAAAAAAG GAAAAGTTTT CTGGTACAAA
    GACAAAGAGT TGCTGGAGTA CTCTTACTAT GGTTACCTTA GTCTCGATGA TGATCACATG
    AGTATAATTG CCAATGCCAT AAATGAGGGA CTGTACACCT GCATTGTGAA GAAGAGGGAC
    CGCATTCTCA CAACTTACTC ATGGAACATA CGACTGCAAA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761991.1
    CDS586..1710
    Translation

    Target ORF information:

    RefSeq Version XM_031906131.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis matrix metallopeptidase 23B (mmp23b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906131.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGGTGACA TCCAGGAAAT TGAGCATTGG CAGAAAAGGT ACATCTGGAC ATTTCTTGCC 
    ATTTTTGCTG GAACAGTGCT GGTAGCTGGA ATTTTCACAG CATCTAATTC AGTGTCCTTG
    GACTCACAGG TAGACTTTGT TGTGGCTCCA ACCCCGGCTC TTCAACTGCC CCTTCAGCTC
    CCTCGCCACC TGCGGAACAA GCGTTACACA TTGACTCCAG GTCTTCTGAA ATGGGATCAT
    TATAACCTGA CATACAGGAA TTTGATCAAT GAAAGTGACA CAAAGAAGGG GATGGCCCAG
    GCTTTCCAGA TGTGGAGTGA AGTGTCTCCT TTTCACTTCA AGGAAGTCCC TGCAGACCAA
    CCAAGTGACC TAGTAATAGG TTTCTATGGT ATCAATCACA CAGACTGCTT GGAGTCCTAT
    ATCCATTATT GCTTTGATGG AACAACGGGC GAGTTGGCAC ATGCGTACTT CCCTAAAACA
    GGAGAAATAC ATTTTGATGA CAGTGAATTT TGGATTTTGG GTAACACGAG GTTCAGTTGG
    AAGAAAGGGG TGTGGCTCAC AGACTTGGTT CACGTGGCAG CCCATGAGAT TGGCCATGCG
    TTGGGACTGA TGCATTCCCT CAATTCTAAT GCTTTGATGC ACATCAATGC AACTCTGACC
    GGAAAAAAAC TGATTTCGCA GGATGATATA TGGGGAATCT ACAGTCTTTA TGGTTGCAAG
    GATAAATATT TAGTGTGTGC CTCCTGGGCC CACAAGGGTT ACTGTGACTC TAGAAAGAGA
    ATTATGAAGA AGTATTGCCC TTACAGCTGT GACTTCTGCT ATGACTTCCC ATTTCCAACC
    AAACTGCCTA CACCTCCACC TCCTAGAACG AAAGTCCGAT TGGTTCCTGA AGGCAGAAAT
    GTTACTTTAC GGTGTGGGAA AAAAATTATG CATAAAAAAG GAAAAGTTTT CTGGTACAAA
    GACAAAGAGT TGCTGGAGTA CTCTTACTAT GGTTACCTTA GTCTCGATGA TGATCACATG
    AGTATAATTG CCAATGCCAT AAATGAGGGA CTGTACACCT GCATTGTGAA GAAGAGGGAC
    CGCATTCTCA CAACTTACTC ATGGAACATA CGACTGCAAA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.