LOC100495742 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100495742
Entrez Gene ID	100495742
Full Name	tolloid like 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012955643.2	XP_012811097.1	tolloid-like protein 1 isoform X2
XM_031895465.1	XP_031751325.1	tolloid-like protein 1
XM_004911165.3	XP_004911222.1	tolloid-like protein 1 isoform X1
XM_002934753.4	XP_002934799.2	tolloid-like protein 1 isoform X1
XM_031895464.1	XP_031751324.1	tolloid-like protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TLL1	NP_036596.3
	TLL1	XP_001101883.2
Gallus gallus (chicken)	TLL1	NP_990034.1
Danio rerio (zebrafish)	tll1	NP_571085.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003702	XP_313494.5
Caenorhabditis elegans (roundworm)	nas-39	NP_510672.2
Drosophila melanogaster (fruit fly)	tok	NP_733035.1
Bos taurus (cattle)	TLL1	NP_001180043.1
Mus musculus (house mouse)	Tll1	NP_033416.2
Canis lupus familiaris (dog)	TLL1	XP_539791.2
Pan troglodytes (chimpanzee)	TLL1	XP_003310613.1
Rattus norvegicus (Norway rat)	Tll1	NP_001099551.1
Xenopus tropicalis (tropical clawed frog)	LOC100495742	XP_004911222.1

Gene Ontology

The following LOC100495742 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100495742 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04646	XM_004911165.3 Latest version!	Xenopus tropicalis tolloid like 1 (tll1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa04646	XM_002934753.4 Latest version!	Xenopus tropicalis tolloid like 1 (tll1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa36365	XM_012955643.2 Latest version!	Xenopus tropicalis tolloid like 1 (tll1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa49820	XM_031895464.1 Latest version!	Xenopus tropicalis tolloid like 1 (LOC100495742), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa49820	XM_031895465.1 Latest version!	Xenopus tropicalis tolloid like 1 (LOC100495742), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100495742 ( XM_004911165.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911222.1 Xenopus tropicalis tolloid like 1 (tll1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa04646
Clone ID Related Accession (Same CDS sequence)	XM_004911165.3 , XM_002934753.4
Accession Version	XM_004911165.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tolloid-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGC CTTCTTGGCT CATTTTCCTC GTTACTGGCT GGACTTTCTG TGACAAGTTA 
TTTGTGTGCA GTGGCCTTGA CTATGATTAT CCTATTTATG ACAACGAAGA GGAAAAAAAT 
GAGGCCATCG ATTACAAAGA TCCTTGCAAA GCCGTTGTCT TTTGGGGAGA TATTGCCTTA 
GATGAAGAAG ATTTAAAAAT GTTTCACATA GACAGGACGA TTGACCTGAC ACAACATTCG 
AATGAAAAGC TGGGACACAC CACAGGTGGC CTTGAAGAAC ATGACCTATC AAAAAGGAGA 
GGAGCCCTCT ATCAGCTTAT TGAGAGGATA AGAAGATTTG GCTCTGAAAA TGCAACAGCA 
AATTCACAAA AAGCAGATAC TACACAGTCT TGGAAGAGTA AGAAAATTAG AACACCTAGA 
GCAGCTACGT CACGAACAGA GAGAATATGG CCCGGAGGAG TTATACCGTA TGTCATTGGG 
GGCAATTTCA CTGGCAGCCA GAGAGCAATG TTTAAGCAGG CCATGAGGCA TTGGGAAAAA 
CATACCTGTG TAACATTCAT AGAACGAACA GATGAAGAGA GCTACATTGT GTTTACTTAC 
AGGCCTTGTG GTTGCTGTTC ATATGTGGGA AGAAGAGGAA ATGGCCCCCA AGCAATATCA 
ATTGGCAAAA ACTGCGATAA ATTTGGAATA GTTGTCCATG AGCTAGGCCA CGTGATTGGC 
TTTTGGCATG AACACACTCG ACCAGACAGA GATGACCATG TGACCATAAT CAGAGAAAAC 
ATTCAACCAG GACAAGAATA CAATTTTCTG AAAATGGAAC CAGGGGAAGT GAATTCACTG 
GGAGAATCAT ATGATTTTGA CAGCATCATG CATTATGCAA GGAACACCTT CTCAAGGGGT 
ATGTTTTTGG ATACAATTCT TCCTTCCCGA GATGAAAATG GTTTAAGGCC CCCTATTGGT 
CAACGAACCC GACTAAGCAA AGGAGATATT GCACAAGCAA GAAAACTCTA CAGATGTCCA 
GCTTGTGGAG AAACTTTGCA AGAATCTACA GGGAATTTTT CATCACCTGG GTTTCCAAAT 
GGTTACCCTT CCTATACTCA CTGCATATGG AGGATTTCTG TAACTCCCGG AGAAAAGATT 
GTACTAAACT TCACCACAAT GGACCTATAC AAAAGCAGTC TGTGCTGGTA TGACTATATA 
GAAGTAAGAG ATGGATACTG GAAAAAATCG CCTCTACTTG GTAGGTTCTG TGGAGACAAA 
CTCCCCGAAG TTCTTACAAG CACAGACAGC AGAATGTGGA TAGAGTTCCG CAGCAGCAGC 
AACTGGGTGG GGAAAGGCTT TGCAGCTGTG TATGAAGCAA TATGTGGAGG TGAGATCCAT 
AAAGACGCTG GCCAGATCCA GTCTCCTAAT TATCCAGATG ATTACCGGCC GCTGAAGGAA 
TGCGTGTGGA AAATAACAGT GGCAGAAAAT TACAATGTTG GGTTAACGTT TCAGGCATTT 
GAGATAGAGC GCCATGACAG TTGTGCTTAT GATTACCTGG AAGTGAGAGA TGGAAGCAGT 
GAAAACAGTC CTCTGATTGG CCATTTTTGT GGTTATGATA AACCAGAGGA CATTCGATCT 
ACATCTAATA CCCTATGGAT GAAATTTGTT TCTGATGGGA CAGTAAACAA AGCAGGGTTT 
GCTGCTAACT TTCTAAAAGA GGAAGATGAA TGCGCTAGGC CAGACAATGG AGGATGTGAG 
CAGCGCTGTG TGAACACCCT GGGAAGTTAT AAGTGCTCTT GTGATCCTGG CTATGAGCTT 
GGACCAGACA AAAAAAGCTG TGAAGCTGCT TGTGGAGGTC TTTTAACGAA ACTCAATGGA 
ACAATCACTA CACCAGCTTG GCCAAAAGAA TACCCTCCAA ACAAAAACTG TGTGTGGCAG 
GTTGTGGCTC CCTCTCAGTA CAGAATCTCT ATGAAATTTG ATTATTTTGA GTTAGAAGGC 
AATGAGGTGT GCAAGTATGA CTATGTTGAG GTTCGAAGTG GCCTCTCCTC TGATTCCAAG 
CTACATGGGA AATTTTGTGG GACAGAGGTA CCTGAAGTAA TAACATCTCA GTATAACAAC 
ATGAGGATTG AATTTAAGTC TGACAACACA GTATCAAAAA AGGGCTTTCG GGCACACTTC 
TTTTCAGATA AAGATGAGTG CTCAAAAGAC AATGGTGGCT GCCAACATGA ATGCATTAAC 
ACCATCGGAA GCTACGTTTG TCAGTGCCGC AATGGCTTTG TTCTTCATGA GAATAAACAC 
GACTGCAAGG AAGCTGAGTG TGAACACAGA ATACACAGCC CAAATGGAGT TATCACAAGC 
CCCAACTGGC CAGACAAGTA TCCGAGTCGC AAGGAATGTA CTTGGGAGAT CAGTGCAACT 
CCCGGACACA GAGTAAAACT TTCTTTCAGC GAATTTGAGA TTGAGCAGCA CCAGGAATGT 
GCTTACGACC ATTTGGAAGT GTTTGATGGA GAAACAGAAA AATCGTCAAT TTTAGGACGC 
CTATGTGGGA GTAAGATCCC AGAGCCTCTG GTTGCCACAG GAAATAAAAT GTTTCTCCGG 
TTTGTGTCTG ATGCATCAGT ACAACGGAAA GGCTTTCAAG CTACCCATTC CACAGGTTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911222.1
CDS	705..3347
Translation	MNMPSWLIFLVTGWTFCDKLFVCSGLDYDYPIYDNEEEKNEAIDYKDPCKAVVFWGDIALDEEDLKMFHIDRTIDLTQHSNEKLGHTTGGLEEHDLSKRRGALYQLIERIRRFGSENATANSQKADTTQSWKSKKIRTPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERTDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIRENIQPGQEYNFLKMEPGEVNSLGESYDFDSIMHYARNTFSRGMFLDTILPSRDENGLRPPIGQRTRLSKGDIAQARKLYRCPACGETLQESTGNFSSPGFPNGYPSYTHCIWRISVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWKKSPLLGRFCGDKLPEVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIHKDAGQIQSPNYPDDYRPLKECVWKITVAENYNVGLTFQAFEIERHDSCAYDYLEVRDGSSENSPLIGHFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFLKEEDECARPDNGGCEQRCVNTLGSYKCSCDPGYELGPDKKSCEAACGGLLTKLNGTITTPAWPKEYPPNKNCVWQVVAPSQYRISMKFDYFELEGNEVCKYDYVEVRSGLSSDSKLHGKFCGTEVPEVITSQYNNMRIEFKSDNTVSKKGFRAHFFSDKDECSKDNGGCQHECINTIGSYVCQCRNGFVLHENKHDCKEAECEHRIHSPNGVITSPNWPDKYPSRKECTWEISATPGHRVKLSFSEFEIEQHQECAYDHLEVFDGETEKSSILGRLCGSKIPEPLVATGNKMFLRFVSDASVQRKGFQATHSTGL

Target ORF information:

RefSeq Version	XM_004911165.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tolloid like 1 (tll1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911165.3

ORF Insert Sequence:

ATGAACATGC CTTCTTGGCT CATTTTCCTC GTTACTGGCT GGACTTTCTG TGACAAGTTA 
TTTGTGTGCA GTGGCCTTGA CTATGATTAT CCTATTTATG ACAACGAAGA GGAAAAAAAT 
GAGGCCATCG ATTACAAAGA TCCTTGCAAA GCCGTTGTCT TTTGGGGAGA TATTGCCTTA 
GATGAAGAAG ATTTAAAAAT GTTTCACATA GACAGGACGA TTGACCTGAC ACAACATTCG 
AATGAAAAGC TGGGACACAC CACAGGTGGC CTTGAAGAAC ATGACCTATC AAAAAGGAGA 
GGAGCCCTCT ATCAGCTTAT TGAGAGGATA AGAAGATTTG GCTCTGAAAA TGCAACAGCA 
AATTCACAAA AAGCAGATAC TACACAGTCT TGGAAGAGTA AGAAAATTAG AACACCTAGA 
GCAGCTACGT CACGAACAGA GAGAATATGG CCCGGAGGAG TTATACCGTA TGTCATTGGG 
GGCAATTTCA CTGGCAGCCA GAGAGCAATG TTTAAGCAGG CCATGAGGCA TTGGGAAAAA 
CATACCTGTG TAACATTCAT AGAACGAACA GATGAAGAGA GCTACATTGT GTTTACTTAC 
AGGCCTTGTG GTTGCTGTTC ATATGTGGGA AGAAGAGGAA ATGGCCCCCA AGCAATATCA 
ATTGGCAAAA ACTGCGATAA ATTTGGAATA GTTGTCCATG AGCTAGGCCA CGTGATTGGC 
TTTTGGCATG AACACACTCG ACCAGACAGA GATGACCATG TGACCATAAT CAGAGAAAAC 
ATTCAACCAG GACAAGAATA CAATTTTCTG AAAATGGAAC CAGGGGAAGT GAATTCACTG 
GGAGAATCAT ATGATTTTGA CAGCATCATG CATTATGCAA GGAACACCTT CTCAAGGGGT 
ATGTTTTTGG ATACAATTCT TCCTTCCCGA GATGAAAATG GTTTAAGGCC CCCTATTGGT 
CAACGAACCC GACTAAGCAA AGGAGATATT GCACAAGCAA GAAAACTCTA CAGATGTCCA 
GCTTGTGGAG AAACTTTGCA AGAATCTACA GGGAATTTTT CATCACCTGG GTTTCCAAAT 
GGTTACCCTT CCTATACTCA CTGCATATGG AGGATTTCTG TAACTCCCGG AGAAAAGATT 
GTACTAAACT TCACCACAAT GGACCTATAC AAAAGCAGTC TGTGCTGGTA TGACTATATA 
GAAGTAAGAG ATGGATACTG GAAAAAATCG CCTCTACTTG GTAGGTTCTG TGGAGACAAA 
CTCCCCGAAG TTCTTACAAG CACAGACAGC AGAATGTGGA TAGAGTTCCG CAGCAGCAGC 
AACTGGGTGG GGAAAGGCTT TGCAGCTGTG TATGAAGCAA TATGTGGAGG TGAGATCCAT 
AAAGACGCTG GCCAGATCCA GTCTCCTAAT TATCCAGATG ATTACCGGCC GCTGAAGGAA 
TGCGTGTGGA AAATAACAGT GGCAGAAAAT TACAATGTTG GGTTAACGTT TCAGGCATTT 
GAGATAGAGC GCCATGACAG TTGTGCTTAT GATTACCTGG AAGTGAGAGA TGGAAGCAGT 
GAAAACAGTC CTCTGATTGG CCATTTTTGT GGTTATGATA AACCAGAGGA CATTCGATCT 
ACATCTAATA CCCTATGGAT GAAATTTGTT TCTGATGGGA CAGTAAACAA AGCAGGGTTT 
GCTGCTAACT TTCTAAAAGA GGAAGATGAA TGCGCTAGGC CAGACAATGG AGGATGTGAG 
CAGCGCTGTG TGAACACCCT GGGAAGTTAT AAGTGCTCTT GTGATCCTGG CTATGAGCTT 
GGACCAGACA AAAAAAGCTG TGAAGCTGCT TGTGGAGGTC TTTTAACGAA ACTCAATGGA 
ACAATCACTA CACCAGCTTG GCCAAAAGAA TACCCTCCAA ACAAAAACTG TGTGTGGCAG 
GTTGTGGCTC CCTCTCAGTA CAGAATCTCT ATGAAATTTG ATTATTTTGA GTTAGAAGGC 
AATGAGGTGT GCAAGTATGA CTATGTTGAG GTTCGAAGTG GCCTCTCCTC TGATTCCAAG 
CTACATGGGA AATTTTGTGG GACAGAGGTA CCTGAAGTAA TAACATCTCA GTATAACAAC 
ATGAGGATTG AATTTAAGTC TGACAACACA GTATCAAAAA AGGGCTTTCG GGCACACTTC 
TTTTCAGATA AAGATGAGTG CTCAAAAGAC AATGGTGGCT GCCAACATGA ATGCATTAAC 
ACCATCGGAA GCTACGTTTG TCAGTGCCGC AATGGCTTTG TTCTTCATGA GAATAAACAC 
GACTGCAAGG AAGCTGAGTG TGAACACAGA ATACACAGCC CAAATGGAGT TATCACAAGC 
CCCAACTGGC CAGACAAGTA TCCGAGTCGC AAGGAATGTA CTTGGGAGAT CAGTGCAACT 
CCCGGACACA GAGTAAAACT TTCTTTCAGC GAATTTGAGA TTGAGCAGCA CCAGGAATGT 
GCTTACGACC ATTTGGAAGT GTTTGATGGA GAAACAGAAA AATCGTCAAT TTTAGGACGC 
CTATGTGGGA GTAAGATCCC AGAGCCTCTG GTTGCCACAG GAAATAAAAT GTTTCTCCGG 
TTTGTGTCTG ATGCATCAGT ACAACGGAAA GGCTTTCAAG CTACCCATTC CACAGGTTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495742 ( XM_002934753.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934799.2 Xenopus tropicalis tolloid like 1 (tll1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa04646
Clone ID Related Accession (Same CDS sequence)	XM_004911165.3 , XM_002934753.4
Accession Version	XM_002934753.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tolloid-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934753.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGC CTTCTTGGCT CATTTTCCTC GTTACTGGCT GGACTTTCTG TGACAAGTTA 
TTTGTGTGCA GTGGCCTTGA CTATGATTAT CCTATTTATG ACAACGAAGA GGAAAAAAAT 
GAGGCCATCG ATTACAAAGA TCCTTGCAAA GCCGTTGTCT TTTGGGGAGA TATTGCCTTA 
GATGAAGAAG ATTTAAAAAT GTTTCACATA GACAGGACGA TTGACCTGAC ACAACATTCG 
AATGAAAAGC TGGGACACAC CACAGGTGGC CTTGAAGAAC ATGACCTATC AAAAAGGAGA 
GGAGCCCTCT ATCAGCTTAT TGAGAGGATA AGAAGATTTG GCTCTGAAAA TGCAACAGCA 
AATTCACAAA AAGCAGATAC TACACAGTCT TGGAAGAGTA AGAAAATTAG AACACCTAGA 
GCAGCTACGT CACGAACAGA GAGAATATGG CCCGGAGGAG TTATACCGTA TGTCATTGGG 
GGCAATTTCA CTGGCAGCCA GAGAGCAATG TTTAAGCAGG CCATGAGGCA TTGGGAAAAA 
CATACCTGTG TAACATTCAT AGAACGAACA GATGAAGAGA GCTACATTGT GTTTACTTAC 
AGGCCTTGTG GTTGCTGTTC ATATGTGGGA AGAAGAGGAA ATGGCCCCCA AGCAATATCA 
ATTGGCAAAA ACTGCGATAA ATTTGGAATA GTTGTCCATG AGCTAGGCCA CGTGATTGGC 
TTTTGGCATG AACACACTCG ACCAGACAGA GATGACCATG TGACCATAAT CAGAGAAAAC 
ATTCAACCAG GACAAGAATA CAATTTTCTG AAAATGGAAC CAGGGGAAGT GAATTCACTG 
GGAGAATCAT ATGATTTTGA CAGCATCATG CATTATGCAA GGAACACCTT CTCAAGGGGT 
ATGTTTTTGG ATACAATTCT TCCTTCCCGA GATGAAAATG GTTTAAGGCC CCCTATTGGT 
CAACGAACCC GACTAAGCAA AGGAGATATT GCACAAGCAA GAAAACTCTA CAGATGTCCA 
GCTTGTGGAG AAACTTTGCA AGAATCTACA GGGAATTTTT CATCACCTGG GTTTCCAAAT 
GGTTACCCTT CCTATACTCA CTGCATATGG AGGATTTCTG TAACTCCCGG AGAAAAGATT 
GTACTAAACT TCACCACAAT GGACCTATAC AAAAGCAGTC TGTGCTGGTA TGACTATATA 
GAAGTAAGAG ATGGATACTG GAAAAAATCG CCTCTACTTG GTAGGTTCTG TGGAGACAAA 
CTCCCCGAAG TTCTTACAAG CACAGACAGC AGAATGTGGA TAGAGTTCCG CAGCAGCAGC 
AACTGGGTGG GGAAAGGCTT TGCAGCTGTG TATGAAGCAA TATGTGGAGG TGAGATCCAT 
AAAGACGCTG GCCAGATCCA GTCTCCTAAT TATCCAGATG ATTACCGGCC GCTGAAGGAA 
TGCGTGTGGA AAATAACAGT GGCAGAAAAT TACAATGTTG GGTTAACGTT TCAGGCATTT 
GAGATAGAGC GCCATGACAG TTGTGCTTAT GATTACCTGG AAGTGAGAGA TGGAAGCAGT 
GAAAACAGTC CTCTGATTGG CCATTTTTGT GGTTATGATA AACCAGAGGA CATTCGATCT 
ACATCTAATA CCCTATGGAT GAAATTTGTT TCTGATGGGA CAGTAAACAA AGCAGGGTTT 
GCTGCTAACT TTCTAAAAGA GGAAGATGAA TGCGCTAGGC CAGACAATGG AGGATGTGAG 
CAGCGCTGTG TGAACACCCT GGGAAGTTAT AAGTGCTCTT GTGATCCTGG CTATGAGCTT 
GGACCAGACA AAAAAAGCTG TGAAGCTGCT TGTGGAGGTC TTTTAACGAA ACTCAATGGA 
ACAATCACTA CACCAGCTTG GCCAAAAGAA TACCCTCCAA ACAAAAACTG TGTGTGGCAG 
GTTGTGGCTC CCTCTCAGTA CAGAATCTCT ATGAAATTTG ATTATTTTGA GTTAGAAGGC 
AATGAGGTGT GCAAGTATGA CTATGTTGAG GTTCGAAGTG GCCTCTCCTC TGATTCCAAG 
CTACATGGGA AATTTTGTGG GACAGAGGTA CCTGAAGTAA TAACATCTCA GTATAACAAC 
ATGAGGATTG AATTTAAGTC TGACAACACA GTATCAAAAA AGGGCTTTCG GGCACACTTC 
TTTTCAGATA AAGATGAGTG CTCAAAAGAC AATGGTGGCT GCCAACATGA ATGCATTAAC 
ACCATCGGAA GCTACGTTTG TCAGTGCCGC AATGGCTTTG TTCTTCATGA GAATAAACAC 
GACTGCAAGG AAGCTGAGTG TGAACACAGA ATACACAGCC CAAATGGAGT TATCACAAGC 
CCCAACTGGC CAGACAAGTA TCCGAGTCGC AAGGAATGTA CTTGGGAGAT CAGTGCAACT 
CCCGGACACA GAGTAAAACT TTCTTTCAGC GAATTTGAGA TTGAGCAGCA CCAGGAATGT 
GCTTACGACC ATTTGGAAGT GTTTGATGGA GAAACAGAAA AATCGTCAAT TTTAGGACGC 
CTATGTGGGA GTAAGATCCC AGAGCCTCTG GTTGCCACAG GAAATAAAAT GTTTCTCCGG 
TTTGTGTCTG ATGCATCAGT ACAACGGAAA GGCTTTCAAG CTACCCATTC CACAGGTTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934799.2
CDS	701..3343
Translation	MNMPSWLIFLVTGWTFCDKLFVCSGLDYDYPIYDNEEEKNEAIDYKDPCKAVVFWGDIALDEEDLKMFHIDRTIDLTQHSNEKLGHTTGGLEEHDLSKRRGALYQLIERIRRFGSENATANSQKADTTQSWKSKKIRTPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERTDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIRENIQPGQEYNFLKMEPGEVNSLGESYDFDSIMHYARNTFSRGMFLDTILPSRDENGLRPPIGQRTRLSKGDIAQARKLYRCPACGETLQESTGNFSSPGFPNGYPSYTHCIWRISVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWKKSPLLGRFCGDKLPEVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIHKDAGQIQSPNYPDDYRPLKECVWKITVAENYNVGLTFQAFEIERHDSCAYDYLEVRDGSSENSPLIGHFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFLKEEDECARPDNGGCEQRCVNTLGSYKCSCDPGYELGPDKKSCEAACGGLLTKLNGTITTPAWPKEYPPNKNCVWQVVAPSQYRISMKFDYFELEGNEVCKYDYVEVRSGLSSDSKLHGKFCGTEVPEVITSQYNNMRIEFKSDNTVSKKGFRAHFFSDKDECSKDNGGCQHECINTIGSYVCQCRNGFVLHENKHDCKEAECEHRIHSPNGVITSPNWPDKYPSRKECTWEISATPGHRVKLSFSEFEIEQHQECAYDHLEVFDGETEKSSILGRLCGSKIPEPLVATGNKMFLRFVSDASVQRKGFQATHSTGL

Target ORF information:

RefSeq Version	XM_002934753.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tolloid like 1 (tll1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934753.4

ORF Insert Sequence:

ATGAACATGC CTTCTTGGCT CATTTTCCTC GTTACTGGCT GGACTTTCTG TGACAAGTTA 
TTTGTGTGCA GTGGCCTTGA CTATGATTAT CCTATTTATG ACAACGAAGA GGAAAAAAAT 
GAGGCCATCG ATTACAAAGA TCCTTGCAAA GCCGTTGTCT TTTGGGGAGA TATTGCCTTA 
GATGAAGAAG ATTTAAAAAT GTTTCACATA GACAGGACGA TTGACCTGAC ACAACATTCG 
AATGAAAAGC TGGGACACAC CACAGGTGGC CTTGAAGAAC ATGACCTATC AAAAAGGAGA 
GGAGCCCTCT ATCAGCTTAT TGAGAGGATA AGAAGATTTG GCTCTGAAAA TGCAACAGCA 
AATTCACAAA AAGCAGATAC TACACAGTCT TGGAAGAGTA AGAAAATTAG AACACCTAGA 
GCAGCTACGT CACGAACAGA GAGAATATGG CCCGGAGGAG TTATACCGTA TGTCATTGGG 
GGCAATTTCA CTGGCAGCCA GAGAGCAATG TTTAAGCAGG CCATGAGGCA TTGGGAAAAA 
CATACCTGTG TAACATTCAT AGAACGAACA GATGAAGAGA GCTACATTGT GTTTACTTAC 
AGGCCTTGTG GTTGCTGTTC ATATGTGGGA AGAAGAGGAA ATGGCCCCCA AGCAATATCA 
ATTGGCAAAA ACTGCGATAA ATTTGGAATA GTTGTCCATG AGCTAGGCCA CGTGATTGGC 
TTTTGGCATG AACACACTCG ACCAGACAGA GATGACCATG TGACCATAAT CAGAGAAAAC 
ATTCAACCAG GACAAGAATA CAATTTTCTG AAAATGGAAC CAGGGGAAGT GAATTCACTG 
GGAGAATCAT ATGATTTTGA CAGCATCATG CATTATGCAA GGAACACCTT CTCAAGGGGT 
ATGTTTTTGG ATACAATTCT TCCTTCCCGA GATGAAAATG GTTTAAGGCC CCCTATTGGT 
CAACGAACCC GACTAAGCAA AGGAGATATT GCACAAGCAA GAAAACTCTA CAGATGTCCA 
GCTTGTGGAG AAACTTTGCA AGAATCTACA GGGAATTTTT CATCACCTGG GTTTCCAAAT 
GGTTACCCTT CCTATACTCA CTGCATATGG AGGATTTCTG TAACTCCCGG AGAAAAGATT 
GTACTAAACT TCACCACAAT GGACCTATAC AAAAGCAGTC TGTGCTGGTA TGACTATATA 
GAAGTAAGAG ATGGATACTG GAAAAAATCG CCTCTACTTG GTAGGTTCTG TGGAGACAAA 
CTCCCCGAAG TTCTTACAAG CACAGACAGC AGAATGTGGA TAGAGTTCCG CAGCAGCAGC 
AACTGGGTGG GGAAAGGCTT TGCAGCTGTG TATGAAGCAA TATGTGGAGG TGAGATCCAT 
AAAGACGCTG GCCAGATCCA GTCTCCTAAT TATCCAGATG ATTACCGGCC GCTGAAGGAA 
TGCGTGTGGA AAATAACAGT GGCAGAAAAT TACAATGTTG GGTTAACGTT TCAGGCATTT 
GAGATAGAGC GCCATGACAG TTGTGCTTAT GATTACCTGG AAGTGAGAGA TGGAAGCAGT 
GAAAACAGTC CTCTGATTGG CCATTTTTGT GGTTATGATA AACCAGAGGA CATTCGATCT 
ACATCTAATA CCCTATGGAT GAAATTTGTT TCTGATGGGA CAGTAAACAA AGCAGGGTTT 
GCTGCTAACT TTCTAAAAGA GGAAGATGAA TGCGCTAGGC CAGACAATGG AGGATGTGAG 
CAGCGCTGTG TGAACACCCT GGGAAGTTAT AAGTGCTCTT GTGATCCTGG CTATGAGCTT 
GGACCAGACA AAAAAAGCTG TGAAGCTGCT TGTGGAGGTC TTTTAACGAA ACTCAATGGA 
ACAATCACTA CACCAGCTTG GCCAAAAGAA TACCCTCCAA ACAAAAACTG TGTGTGGCAG 
GTTGTGGCTC CCTCTCAGTA CAGAATCTCT ATGAAATTTG ATTATTTTGA GTTAGAAGGC 
AATGAGGTGT GCAAGTATGA CTATGTTGAG GTTCGAAGTG GCCTCTCCTC TGATTCCAAG 
CTACATGGGA AATTTTGTGG GACAGAGGTA CCTGAAGTAA TAACATCTCA GTATAACAAC 
ATGAGGATTG AATTTAAGTC TGACAACACA GTATCAAAAA AGGGCTTTCG GGCACACTTC 
TTTTCAGATA AAGATGAGTG CTCAAAAGAC AATGGTGGCT GCCAACATGA ATGCATTAAC 
ACCATCGGAA GCTACGTTTG TCAGTGCCGC AATGGCTTTG TTCTTCATGA GAATAAACAC 
GACTGCAAGG AAGCTGAGTG TGAACACAGA ATACACAGCC CAAATGGAGT TATCACAAGC 
CCCAACTGGC CAGACAAGTA TCCGAGTCGC AAGGAATGTA CTTGGGAGAT CAGTGCAACT 
CCCGGACACA GAGTAAAACT TTCTTTCAGC GAATTTGAGA TTGAGCAGCA CCAGGAATGT 
GCTTACGACC ATTTGGAAGT GTTTGATGGA GAAACAGAAA AATCGTCAAT TTTAGGACGC 
CTATGTGGGA GTAAGATCCC AGAGCCTCTG GTTGCCACAG GAAATAAAAT GTTTCTCCGG 
TTTGTGTCTG ATGCATCAGT ACAACGGAAA GGCTTTCAAG CTACCCATTC CACAGGTTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495742 ( XM_012955643.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811097.1 Xenopus tropicalis tolloid like 1 (tll1), transcript variant X3, mRNA.

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CloneID	OXa36365
Clone ID Related Accession (Same CDS sequence)	XM_012955643.2
Accession Version	XM_012955643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tolloid-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCGGA GGAGTTATAC CGTATGTCAT TGGGGGCAAT TTCACTGCCA GAGAGCAATG 
TTTAAGCAGG CCATGAGGCA TTGGGAAAAA CATACCTGTG TAACATTCAT AGAACGAACA 
GATGAAGAGA GCTACATTGT GTTTACTTAC AGGCCTTGTG GTTGCTGTTC ATATGTGGGA 
AGAAGAGGAA ATGGCCCCCA AGCAATATCA ATTGGCAAAA ACTGCGATAA ATTTGGAATA 
GTTGTCCATG AGCTAGGCCA CGTGATTGGC TTTTGGCATG AACACACTCG ACCAGACAGA 
GATGACCATG TGACCATAAT CAGAGAAAAC ATTCAACCAG GACAAGAATA CAATTTTCTG 
AAAATGGAAC CAGGGGAAGT GAATTCACTG GGAGAATCAT ATGATTTTGA CAGCATCATG 
CATTATGCAA GGAACACCTT CTCAAGGGGT ATGTTTTTGG ATACAATTCT TCCTTCCCGA 
GATGAAAATG GTTTAAGGCC CCCTATTGGT CAACGAACCC GACTAAGCAA AGGAGATATT 
GCACAAGCAA GAAAACTCTA CAGATGTCCA GCTTGTGGAG AAACTTTGCA AGAATCTACA 
GGGAATTTTT CATCACCTGG GTTTCCAAAT GGTTACCCTT CCTATACTCA CTGCATATGG 
AGGATTTCTG TAACTCCCGG AGAAAAGATT GTACTAAACT TCACCACAAT GGACCTATAC 
AAAAGCAGTC TGTGCTGGTA TGACTATATA GAAGTAAGAG ATGGATACTG GAAAAAATCG 
CCTCTACTTG GTAGGTTCTG TGGAGACAAA CTCCCCGAAG TTCTTACAAG CACAGACAGC 
AGAATGTGGA TAGAGTTCCG CAGCAGCAGC AACTGGGTGG GGAAAGGCTT TGCAGCTGTG 
TATGAAGCAA TATGTGGAGG TGAGATCCAT AAAGACGCTG GCCAGATCCA GTCTCCTAAT 
TATCCAGATG ATTACCGGCC GCTGAAGGAA TGCGTGTGGA AAATAACAGT GGCAGAAAAT 
TACAATGTTG GGTTAACGTT TCAGGCATTT GAGATAGAGC GCCATGACAG TTGTGCTTAT 
GATTACCTGG AAGTGAGAGA TGGAAGCAGT GAAAACAGTC CTCTGATTGG CCATTTTTGT 
GGTTATGATA AACCAGAGGA CATTCGATCT ACATCTAATA CCCTATGGAT GAAATTTGTT 
TCTGATGGGA CAGTAAACAA AGCAGGGTTT GCTGCTAACT TTCTAAAAGA GGAAGATGAA 
TGCGCTAGGC CAGACAATGG AGGATGTGAG CAGCGCTGTG TGAACACCCT GGGAAGTTAT 
AAGTGCTCTT GTGATCCTGG CTATGAGCTT GGACCAGACA AAAAAAGCTG TGAAGCTGCT 
TGTGGAGGTC TTTTAACGAA ACTCAATGGA ACAATCACTA CACCAGCTTG GCCAAAAGAA 
TACCCTCCAA ACAAAAACTG TGTGTGGCAG GTTGTGGCTC CCTCTCAGTA CAGAATCTCT 
ATGAAATTTG ATTATTTTGA GTTAGAAGGC AATGAGGTGT GCAAGTATGA CTATGTTGAG 
GTTCGAAGTG GCCTCTCCTC TGATTCCAAG CTACATGGGA AATTTTGTGG GACAGAGGTA 
CCTGAAGTAA TAACATCTCA GTATAACAAC ATGAGGATTG AATTTAAGTC TGACAACACA 
GTATCAAAAA AGGGCTTTCG GGCACACTTC TTTTCAGATA AAGATGAGTG CTCAAAAGAC 
AATGGTGGCT GCCAACATGA ATGCATTAAC ACCATCGGAA GCTACGTTTG TCAGTGCCGC 
AATGGCTTTG TTCTTCATGA GAATAAACAC GACTGCAAGG AAGCTGAGTG TGAACACAGA 
ATACACAGCC CAAATGGAGT TATCACAAGC CCCAACTGGC CAGACAAGTA TCCGAGTCGC 
AAGGAATGTA CTTGGGAGAT CAGTGCAACT CCCGGACACA GAGTAAAACT TTCTTTCAGC 
GAATTTGAGA TTGAGCAGCA CCAGGAATGT GCTTACGACC ATTTGGAAGT GTTTGATGGA 
GAAACAGAAA AATCGTCAAT TTTAGGACGC CTATGTGGGA GTAAGATCCC AGAGCCTCTG 
GTTGCCACAG GAAATAAAAT GTTTCTCCGG TTTGTGTCTG ATGCATCAGT ACAACGGAAA 
GGCTTTCAAG CTACCCATTC CACAGGTTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811097.1
CDS	95..2287
Translation	MARRSYTVCHWGQFHCQRAMFKQAMRHWEKHTCVTFIERTDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIRENIQPGQEYNFLKMEPGEVNSLGESYDFDSIMHYARNTFSRGMFLDTILPSRDENGLRPPIGQRTRLSKGDIAQARKLYRCPACGETLQESTGNFSSPGFPNGYPSYTHCIWRISVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWKKSPLLGRFCGDKLPEVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIHKDAGQIQSPNYPDDYRPLKECVWKITVAENYNVGLTFQAFEIERHDSCAYDYLEVRDGSSENSPLIGHFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFLKEEDECARPDNGGCEQRCVNTLGSYKCSCDPGYELGPDKKSCEAACGGLLTKLNGTITTPAWPKEYPPNKNCVWQVVAPSQYRISMKFDYFELEGNEVCKYDYVEVRSGLSSDSKLHGKFCGTEVPEVITSQYNNMRIEFKSDNTVSKKGFRAHFFSDKDECSKDNGGCQHECINTIGSYVCQCRNGFVLHENKHDCKEAECEHRIHSPNGVITSPNWPDKYPSRKECTWEISATPGHRVKLSFSEFEIEQHQECAYDHLEVFDGETEKSSILGRLCGSKIPEPLVATGNKMFLRFVSDASVQRKGFQATHSTGL

Target ORF information:

RefSeq Version	XM_012955643.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tolloid like 1 (tll1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955643.2

ORF Insert Sequence:

ATGGCCCGGA GGAGTTATAC CGTATGTCAT TGGGGGCAAT TTCACTGCCA GAGAGCAATG 
TTTAAGCAGG CCATGAGGCA TTGGGAAAAA CATACCTGTG TAACATTCAT AGAACGAACA 
GATGAAGAGA GCTACATTGT GTTTACTTAC AGGCCTTGTG GTTGCTGTTC ATATGTGGGA 
AGAAGAGGAA ATGGCCCCCA AGCAATATCA ATTGGCAAAA ACTGCGATAA ATTTGGAATA 
GTTGTCCATG AGCTAGGCCA CGTGATTGGC TTTTGGCATG AACACACTCG ACCAGACAGA 
GATGACCATG TGACCATAAT CAGAGAAAAC ATTCAACCAG GACAAGAATA CAATTTTCTG 
AAAATGGAAC CAGGGGAAGT GAATTCACTG GGAGAATCAT ATGATTTTGA CAGCATCATG 
CATTATGCAA GGAACACCTT CTCAAGGGGT ATGTTTTTGG ATACAATTCT TCCTTCCCGA 
GATGAAAATG GTTTAAGGCC CCCTATTGGT CAACGAACCC GACTAAGCAA AGGAGATATT 
GCACAAGCAA GAAAACTCTA CAGATGTCCA GCTTGTGGAG AAACTTTGCA AGAATCTACA 
GGGAATTTTT CATCACCTGG GTTTCCAAAT GGTTACCCTT CCTATACTCA CTGCATATGG 
AGGATTTCTG TAACTCCCGG AGAAAAGATT GTACTAAACT TCACCACAAT GGACCTATAC 
AAAAGCAGTC TGTGCTGGTA TGACTATATA GAAGTAAGAG ATGGATACTG GAAAAAATCG 
CCTCTACTTG GTAGGTTCTG TGGAGACAAA CTCCCCGAAG TTCTTACAAG CACAGACAGC 
AGAATGTGGA TAGAGTTCCG CAGCAGCAGC AACTGGGTGG GGAAAGGCTT TGCAGCTGTG 
TATGAAGCAA TATGTGGAGG TGAGATCCAT AAAGACGCTG GCCAGATCCA GTCTCCTAAT 
TATCCAGATG ATTACCGGCC GCTGAAGGAA TGCGTGTGGA AAATAACAGT GGCAGAAAAT 
TACAATGTTG GGTTAACGTT TCAGGCATTT GAGATAGAGC GCCATGACAG TTGTGCTTAT 
GATTACCTGG AAGTGAGAGA TGGAAGCAGT GAAAACAGTC CTCTGATTGG CCATTTTTGT 
GGTTATGATA AACCAGAGGA CATTCGATCT ACATCTAATA CCCTATGGAT GAAATTTGTT 
TCTGATGGGA CAGTAAACAA AGCAGGGTTT GCTGCTAACT TTCTAAAAGA GGAAGATGAA 
TGCGCTAGGC CAGACAATGG AGGATGTGAG CAGCGCTGTG TGAACACCCT GGGAAGTTAT 
AAGTGCTCTT GTGATCCTGG CTATGAGCTT GGACCAGACA AAAAAAGCTG TGAAGCTGCT 
TGTGGAGGTC TTTTAACGAA ACTCAATGGA ACAATCACTA CACCAGCTTG GCCAAAAGAA 
TACCCTCCAA ACAAAAACTG TGTGTGGCAG GTTGTGGCTC CCTCTCAGTA CAGAATCTCT 
ATGAAATTTG ATTATTTTGA GTTAGAAGGC AATGAGGTGT GCAAGTATGA CTATGTTGAG 
GTTCGAAGTG GCCTCTCCTC TGATTCCAAG CTACATGGGA AATTTTGTGG GACAGAGGTA 
CCTGAAGTAA TAACATCTCA GTATAACAAC ATGAGGATTG AATTTAAGTC TGACAACACA 
GTATCAAAAA AGGGCTTTCG GGCACACTTC TTTTCAGATA AAGATGAGTG CTCAAAAGAC 
AATGGTGGCT GCCAACATGA ATGCATTAAC ACCATCGGAA GCTACGTTTG TCAGTGCCGC 
AATGGCTTTG TTCTTCATGA GAATAAACAC GACTGCAAGG AAGCTGAGTG TGAACACAGA 
ATACACAGCC CAAATGGAGT TATCACAAGC CCCAACTGGC CAGACAAGTA TCCGAGTCGC 
AAGGAATGTA CTTGGGAGAT CAGTGCAACT CCCGGACACA GAGTAAAACT TTCTTTCAGC 
GAATTTGAGA TTGAGCAGCA CCAGGAATGT GCTTACGACC ATTTGGAAGT GTTTGATGGA 
GAAACAGAAA AATCGTCAAT TTTAGGACGC CTATGTGGGA GTAAGATCCC AGAGCCTCTG 
GTTGCCACAG GAAATAAAAT GTTTCTCCGG TTTGTGTCTG ATGCATCAGT ACAACGGAAA 
GGCTTTCAAG CTACCCATTC CACAGGTTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495742 ( XM_031895465.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751325.1 Xenopus tropicalis tolloid like 1 (LOC100495742), transcript variant X2, mRNA.

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CloneID	OXa49820
Clone ID Related Accession (Same CDS sequence)	XM_031895465.1 , XM_031895464.1
Accession Version	XM_031895465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tolloid-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGC CTTCTTGGCT CATTTTCCTC GTTACTGGCT GGACTTTCTG TGACAAGTTA 
TTTGTGTGCA GTGGCCTTGA CTATGATTAT CCTATTTATG ACAACGAAGA GGAAAAAAAT 
GAGGCCATCG ATTACAAAGA TCCTTGCAAA GCCGTTGTCT TTTGGGGAGA TATTGCCTTA 
GATGAAGAAG ATTTAAAAAT GTTTCACATA GACAGGACGA TTGACCTGAC ACAACATTCG 
AATGAAAAGC TGGGACACAC CACAGGTGGC CTTGAAGAAC ATGACCTATC AAAAAGGAGA 
GGAGCCCTCT ATCAGCTTAT TGAGAGGATA AGAAGATTTG GCTCTGAAAA TGCAACAGCA 
AATTCACAAA AAGCAGATAC TACACAGTCT TGGAAGAGTA AGAAAATTAG AACACCTAGA 
GCAGCTACGT CACGAACAGA GAGAATATGG CCCGGAGGAG TTATACCGTA TGTCATTGGG 
GGCAATTTCA CTGGCAGCCA GAGAGCAATG TTTAAGCAGG CCATGAGGCA TTGGGAAAAA 
CATACCTGTG TAACATTCAT AGAACGAACA GATGAAGAGA GCTACATTGT GTTTACTTAC 
AGGCCTTGTG GTTGCTGTTC ATATGTGGGA AGAAGAGGAA ATGGCCCCCA AGCAATATCA 
ATTGGCAAAA ACTGCGATAA ATTTGGAATA GTTGTCCATG AGCTAGGCCA CGTGATTGGC 
TTTTGGCATG AACACACTCG ACCAGACAGA GATGACCATG TGACCATAAT CAGAGAAAAC 
ATTCAACCAG GACAAGAATA CAATTTTCTG AAAATGGAAC CAGGGGAAGT GAATTCACTG 
GGAGAATCAT ATGATTTTGA CAGCATCATG CATTATGCAA GGAACACCTT CTCAAGGGGT 
ATGTTTTTGG ATACAATTCT TCCTTCCCGA GATGAAAATG GTTTAAGGCC CCCTATTGGT 
CAACGAACCC GACTAAGCAA AGGAGATATT GCACAAGCAA GAAAACTCTA CAGATGTCCA 
GCTTGTGGAG AAACTTTGCA AGAATCTACA GGGAATTTTT CATCACCTGG GTTTCCAAAT 
GGTTACCCTT CCTATACTCA CTGCATATGG AGGATTTCTG TAACTCCCGG AGAAAAGATT 
GTACTAAACT TCACCACAAT GGACCTATAC AAAAGCAGTC TGTGCTGGTA TGACTATATA 
GAAGTAAGAG ATGGATACTG GAAAAAATCG CCTCTACTTG GTAGGTTCTG TGGAGACAAA 
CTCCCCGAAG TTCTTACAAG CACAGACAGC AGAATGTGGA TAGAGTTCCG CAGCAGCAGC 
AACTGGGTGG GGAAAGGCTT TGCAGCTGTG TATGAAGCAA TATGTGGAGG TGAGATCCAT 
AAAGACGCTG GCCAGATCCA GTCTCCTAAT TATCCAGATG ATTACCGGCC GCTGAAGGAA 
TGCGTGTGGA AAATAACAGT GGCAGAAAAT TACAATGTTG GGTTAACGTT TCAGGCATTT 
GAGATAGAGC GCCATGACAG TTGTGCTTAT GATTACCTGG AAGTGAGAGA TGGAAGCAGT 
GAAAACAGTC CTCTGATTGG CCATTTTTGT GGTTATGATA AACCAGAGGA CATTCGATCT 
ACATCTAATA CCCTATGGAT GAAATTTGTT TCTGATGGGA CAGTAAACAA AGCAGGGTTT 
GCTGCTAACT TTCTAAAAGA GGAAGATGAA TGCGCTAGGC CAGACAATGG AGGATGTGAG 
CAGCGCTGTG TGAACACCCT GGGAAGTTAT AAGTGCTCTT GTGATCCTGG CTATGAGCTT 
GGACCAGACA AAAAAAGCTG TGAAGCTGCT TGTGGAGGTC TTTTAACGAA ACTCAATGGA 
ACAATCACTA CACCAGCTTG GCCAAAAGAA TACCCTCCAA ACAAAAACTG TGTGTGGCAG 
GTTGTGGCTC CCTCTCAGTA CAGAATCTCT ATGAAATTTG ATTATTTTGA GTTAGAAGGC 
AATGAGGTGT GCAAGTATGA CTATGTTGAG GTTCGAAGTG GCCTCTCCTC TGATTCCAAG 
CTACATGGGA AATTTTGTGG GACAGAGGTA CCTGAAGTAA TAACATCTCA GTATAACAAC 
ATGAGGATTG AATTTAAGTC TGACAACACA GTATCAAAAA AGGGCTTTCG GGCACACTTC 
TTTTCAGATA AAGATGAGTG CTCAAAAGAC AATGGTGGCT GCCAACATGA ATGCATTAAC 
ACCATCGGAA GCTACGTTTG TCAGTGCCGC AATGGCTTTG TTCTTCATGA GAATAAACAC 
GACTGCAAGG AAGCTGAGTG TGAACACAGA ATACACAGCC CAAATGGAGT TATCACAAGC 
CCCAACTGGC CAGACAAGTA TCCGAGTCGC AAGGAATGTA CTTGGGAGAT CAGTGCAACT 
CCCGGACACA GAGTAAAACT TTCTTTCAGC GAATTTGAGA TTGAGCAGCA CCAGGAATGT 
GCTTACGACC ATTTGGAAGT GTTTGATGGA GAAACAGAAA AATCGTCAAT TTTAGGACGC 
CTATGTGGGA GTAAGATCCC AGAGCCTCTG GTTGCCACAG GAAATAAAAT GTTTCTCCGG 
TTTGTGTCTG ATGCATCAGT ACAACGGAAA GGCTTTCAAG CTACCCATTC CACAGAGTGC 
GGCGGCAGGC TGAAAGCAGA AGCAAAGTCG AGAGACCTGT TCTCCCATGC CCAGTTTGGT 
GACAACAATT ACCCAGTGCA AGCGGATTGC GAGTGGATGT TAGCGACAGA GAGGGGTCTG 
CGCATTGAGC TCAGCTTTCA GACATTTGAA GTGGAGGAAG AAGCTGACTG TGGCTATGAC 
TTTATGGAGC TGTTTGATGG GCATGATGCG TCAGCCATGA GACTTGGGCG TTTCTGTGGA 
TCAGGGCCTC CTGAAGAAAT CATATCCACT GGCGATGCCA TATTAATTCA CTTTCACACC 
GATGACACAA TCAGCAAGAA AGGATTTCAT ATACGATACA AAAGCGTGAA ATATCAAGAC 
ATTCTACACA CCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751325.1
CDS	678..3695
Translation	MNMPSWLIFLVTGWTFCDKLFVCSGLDYDYPIYDNEEEKNEAIDYKDPCKAVVFWGDIALDEEDLKMFHIDRTIDLTQHSNEKLGHTTGGLEEHDLSKRRGALYQLIERIRRFGSENATANSQKADTTQSWKSKKIRTPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERTDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIRENIQPGQEYNFLKMEPGEVNSLGESYDFDSIMHYARNTFSRGMFLDTILPSRDENGLRPPIGQRTRLSKGDIAQARKLYRCPACGETLQESTGNFSSPGFPNGYPSYTHCIWRISVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWKKSPLLGRFCGDKLPEVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIHKDAGQIQSPNYPDDYRPLKECVWKITVAENYNVGLTFQAFEIERHDSCAYDYLEVRDGSSENSPLIGHFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFLKEEDECARPDNGGCEQRCVNTLGSYKCSCDPGYELGPDKKSCEAACGGLLTKLNGTITTPAWPKEYPPNKNCVWQVVAPSQYRISMKFDYFELEGNEVCKYDYVEVRSGLSSDSKLHGKFCGTEVPEVITSQYNNMRIEFKSDNTVSKKGFRAHFFSDKDECSKDNGGCQHECINTIGSYVCQCRNGFVLHENKHDCKEAECEHRIHSPNGVITSPNWPDKYPSRKECTWEISATPGHRVKLSFSEFEIEQHQECAYDHLEVFDGETEKSSILGRLCGSKIPEPLVATGNKMFLRFVSDASVQRKGFQATHSTECGGRLKAEAKSRDLFSHAQFGDNNYPVQADCEWMLATERGLRIELSFQTFEVEEEADCGYDFMELFDGHDASAMRLGRFCGSGPPEEIISTGDAILIHFHTDDTISKKGFHIRYKSVKYQDILHTK

Target ORF information:

RefSeq Version	XM_031895465.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tolloid like 1 (LOC100495742), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895465.1

ORF Insert Sequence:

ATGAACATGC CTTCTTGGCT CATTTTCCTC GTTACTGGCT GGACTTTCTG TGACAAGTTA 
TTTGTGTGCA GTGGCCTTGA CTATGATTAT CCTATTTATG ACAACGAAGA GGAAAAAAAT 
GAGGCCATCG ATTACAAAGA TCCTTGCAAA GCCGTTGTCT TTTGGGGAGA TATTGCCTTA 
GATGAAGAAG ATTTAAAAAT GTTTCACATA GACAGGACGA TTGACCTGAC ACAACATTCG 
AATGAAAAGC TGGGACACAC CACAGGTGGC CTTGAAGAAC ATGACCTATC AAAAAGGAGA 
GGAGCCCTCT ATCAGCTTAT TGAGAGGATA AGAAGATTTG GCTCTGAAAA TGCAACAGCA 
AATTCACAAA AAGCAGATAC TACACAGTCT TGGAAGAGTA AGAAAATTAG AACACCTAGA 
GCAGCTACGT CACGAACAGA GAGAATATGG CCCGGAGGAG TTATACCGTA TGTCATTGGG 
GGCAATTTCA CTGGCAGCCA GAGAGCAATG TTTAAGCAGG CCATGAGGCA TTGGGAAAAA 
CATACCTGTG TAACATTCAT AGAACGAACA GATGAAGAGA GCTACATTGT GTTTACTTAC 
AGGCCTTGTG GTTGCTGTTC ATATGTGGGA AGAAGAGGAA ATGGCCCCCA AGCAATATCA 
ATTGGCAAAA ACTGCGATAA ATTTGGAATA GTTGTCCATG AGCTAGGCCA CGTGATTGGC 
TTTTGGCATG AACACACTCG ACCAGACAGA GATGACCATG TGACCATAAT CAGAGAAAAC 
ATTCAACCAG GACAAGAATA CAATTTTCTG AAAATGGAAC CAGGGGAAGT GAATTCACTG 
GGAGAATCAT ATGATTTTGA CAGCATCATG CATTATGCAA GGAACACCTT CTCAAGGGGT 
ATGTTTTTGG ATACAATTCT TCCTTCCCGA GATGAAAATG GTTTAAGGCC CCCTATTGGT 
CAACGAACCC GACTAAGCAA AGGAGATATT GCACAAGCAA GAAAACTCTA CAGATGTCCA 
GCTTGTGGAG AAACTTTGCA AGAATCTACA GGGAATTTTT CATCACCTGG GTTTCCAAAT 
GGTTACCCTT CCTATACTCA CTGCATATGG AGGATTTCTG TAACTCCCGG AGAAAAGATT 
GTACTAAACT TCACCACAAT GGACCTATAC AAAAGCAGTC TGTGCTGGTA TGACTATATA 
GAAGTAAGAG ATGGATACTG GAAAAAATCG CCTCTACTTG GTAGGTTCTG TGGAGACAAA 
CTCCCCGAAG TTCTTACAAG CACAGACAGC AGAATGTGGA TAGAGTTCCG CAGCAGCAGC 
AACTGGGTGG GGAAAGGCTT TGCAGCTGTG TATGAAGCAA TATGTGGAGG TGAGATCCAT 
AAAGACGCTG GCCAGATCCA GTCTCCTAAT TATCCAGATG ATTACCGGCC GCTGAAGGAA 
TGCGTGTGGA AAATAACAGT GGCAGAAAAT TACAATGTTG GGTTAACGTT TCAGGCATTT 
GAGATAGAGC GCCATGACAG TTGTGCTTAT GATTACCTGG AAGTGAGAGA TGGAAGCAGT 
GAAAACAGTC CTCTGATTGG CCATTTTTGT GGTTATGATA AACCAGAGGA CATTCGATCT 
ACATCTAATA CCCTATGGAT GAAATTTGTT TCTGATGGGA CAGTAAACAA AGCAGGGTTT 
GCTGCTAACT TTCTAAAAGA GGAAGATGAA TGCGCTAGGC CAGACAATGG AGGATGTGAG 
CAGCGCTGTG TGAACACCCT GGGAAGTTAT AAGTGCTCTT GTGATCCTGG CTATGAGCTT 
GGACCAGACA AAAAAAGCTG TGAAGCTGCT TGTGGAGGTC TTTTAACGAA ACTCAATGGA 
ACAATCACTA CACCAGCTTG GCCAAAAGAA TACCCTCCAA ACAAAAACTG TGTGTGGCAG 
GTTGTGGCTC CCTCTCAGTA CAGAATCTCT ATGAAATTTG ATTATTTTGA GTTAGAAGGC 
AATGAGGTGT GCAAGTATGA CTATGTTGAG GTTCGAAGTG GCCTCTCCTC TGATTCCAAG 
CTACATGGGA AATTTTGTGG GACAGAGGTA CCTGAAGTAA TAACATCTCA GTATAACAAC 
ATGAGGATTG AATTTAAGTC TGACAACACA GTATCAAAAA AGGGCTTTCG GGCACACTTC 
TTTTCAGATA AAGATGAGTG CTCAAAAGAC AATGGTGGCT GCCAACATGA ATGCATTAAC 
ACCATCGGAA GCTACGTTTG TCAGTGCCGC AATGGCTTTG TTCTTCATGA GAATAAACAC 
GACTGCAAGG AAGCTGAGTG TGAACACAGA ATACACAGCC CAAATGGAGT TATCACAAGC 
CCCAACTGGC CAGACAAGTA TCCGAGTCGC AAGGAATGTA CTTGGGAGAT CAGTGCAACT 
CCCGGACACA GAGTAAAACT TTCTTTCAGC GAATTTGAGA TTGAGCAGCA CCAGGAATGT 
GCTTACGACC ATTTGGAAGT GTTTGATGGA GAAACAGAAA AATCGTCAAT TTTAGGACGC 
CTATGTGGGA GTAAGATCCC AGAGCCTCTG GTTGCCACAG GAAATAAAAT GTTTCTCCGG 
TTTGTGTCTG ATGCATCAGT ACAACGGAAA GGCTTTCAAG CTACCCATTC CACAGAGTGC 
GGCGGCAGGC TGAAAGCAGA AGCAAAGTCG AGAGACCTGT TCTCCCATGC CCAGTTTGGT 
GACAACAATT ACCCAGTGCA AGCGGATTGC GAGTGGATGT TAGCGACAGA GAGGGGTCTG 
CGCATTGAGC TCAGCTTTCA GACATTTGAA GTGGAGGAAG AAGCTGACTG TGGCTATGAC 
TTTATGGAGC TGTTTGATGG GCATGATGCG TCAGCCATGA GACTTGGGCG TTTCTGTGGA 
TCAGGGCCTC CTGAAGAAAT CATATCCACT GGCGATGCCA TATTAATTCA CTTTCACACC 
GATGACACAA TCAGCAAGAA AGGATTTCAT ATACGATACA AAAGCGTGAA ATATCAAGAC 
ATTCTACACA CCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100495742 ( XM_031895464.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751324.1 Xenopus tropicalis tolloid like 1 (LOC100495742), transcript variant X1, mRNA.

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CloneID	OXa49820
Clone ID Related Accession (Same CDS sequence)	XM_031895465.1 , XM_031895464.1
Accession Version	XM_031895464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tolloid-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATGC CTTCTTGGCT CATTTTCCTC GTTACTGGCT GGACTTTCTG TGACAAGTTA 
TTTGTGTGCA GTGGCCTTGA CTATGATTAT CCTATTTATG ACAACGAAGA GGAAAAAAAT 
GAGGCCATCG ATTACAAAGA TCCTTGCAAA GCCGTTGTCT TTTGGGGAGA TATTGCCTTA 
GATGAAGAAG ATTTAAAAAT GTTTCACATA GACAGGACGA TTGACCTGAC ACAACATTCG 
AATGAAAAGC TGGGACACAC CACAGGTGGC CTTGAAGAAC ATGACCTATC AAAAAGGAGA 
GGAGCCCTCT ATCAGCTTAT TGAGAGGATA AGAAGATTTG GCTCTGAAAA TGCAACAGCA 
AATTCACAAA AAGCAGATAC TACACAGTCT TGGAAGAGTA AGAAAATTAG AACACCTAGA 
GCAGCTACGT CACGAACAGA GAGAATATGG CCCGGAGGAG TTATACCGTA TGTCATTGGG 
GGCAATTTCA CTGGCAGCCA GAGAGCAATG TTTAAGCAGG CCATGAGGCA TTGGGAAAAA 
CATACCTGTG TAACATTCAT AGAACGAACA GATGAAGAGA GCTACATTGT GTTTACTTAC 
AGGCCTTGTG GTTGCTGTTC ATATGTGGGA AGAAGAGGAA ATGGCCCCCA AGCAATATCA 
ATTGGCAAAA ACTGCGATAA ATTTGGAATA GTTGTCCATG AGCTAGGCCA CGTGATTGGC 
TTTTGGCATG AACACACTCG ACCAGACAGA GATGACCATG TGACCATAAT CAGAGAAAAC 
ATTCAACCAG GACAAGAATA CAATTTTCTG AAAATGGAAC CAGGGGAAGT GAATTCACTG 
GGAGAATCAT ATGATTTTGA CAGCATCATG CATTATGCAA GGAACACCTT CTCAAGGGGT 
ATGTTTTTGG ATACAATTCT TCCTTCCCGA GATGAAAATG GTTTAAGGCC CCCTATTGGT 
CAACGAACCC GACTAAGCAA AGGAGATATT GCACAAGCAA GAAAACTCTA CAGATGTCCA 
GCTTGTGGAG AAACTTTGCA AGAATCTACA GGGAATTTTT CATCACCTGG GTTTCCAAAT 
GGTTACCCTT CCTATACTCA CTGCATATGG AGGATTTCTG TAACTCCCGG AGAAAAGATT 
GTACTAAACT TCACCACAAT GGACCTATAC AAAAGCAGTC TGTGCTGGTA TGACTATATA 
GAAGTAAGAG ATGGATACTG GAAAAAATCG CCTCTACTTG GTAGGTTCTG TGGAGACAAA 
CTCCCCGAAG TTCTTACAAG CACAGACAGC AGAATGTGGA TAGAGTTCCG CAGCAGCAGC 
AACTGGGTGG GGAAAGGCTT TGCAGCTGTG TATGAAGCAA TATGTGGAGG TGAGATCCAT 
AAAGACGCTG GCCAGATCCA GTCTCCTAAT TATCCAGATG ATTACCGGCC GCTGAAGGAA 
TGCGTGTGGA AAATAACAGT GGCAGAAAAT TACAATGTTG GGTTAACGTT TCAGGCATTT 
GAGATAGAGC GCCATGACAG TTGTGCTTAT GATTACCTGG AAGTGAGAGA TGGAAGCAGT 
GAAAACAGTC CTCTGATTGG CCATTTTTGT GGTTATGATA AACCAGAGGA CATTCGATCT 
ACATCTAATA CCCTATGGAT GAAATTTGTT TCTGATGGGA CAGTAAACAA AGCAGGGTTT 
GCTGCTAACT TTCTAAAAGA GGAAGATGAA TGCGCTAGGC CAGACAATGG AGGATGTGAG 
CAGCGCTGTG TGAACACCCT GGGAAGTTAT AAGTGCTCTT GTGATCCTGG CTATGAGCTT 
GGACCAGACA AAAAAAGCTG TGAAGCTGCT TGTGGAGGTC TTTTAACGAA ACTCAATGGA 
ACAATCACTA CACCAGCTTG GCCAAAAGAA TACCCTCCAA ACAAAAACTG TGTGTGGCAG 
GTTGTGGCTC CCTCTCAGTA CAGAATCTCT ATGAAATTTG ATTATTTTGA GTTAGAAGGC 
AATGAGGTGT GCAAGTATGA CTATGTTGAG GTTCGAAGTG GCCTCTCCTC TGATTCCAAG 
CTACATGGGA AATTTTGTGG GACAGAGGTA CCTGAAGTAA TAACATCTCA GTATAACAAC 
ATGAGGATTG AATTTAAGTC TGACAACACA GTATCAAAAA AGGGCTTTCG GGCACACTTC 
TTTTCAGATA AAGATGAGTG CTCAAAAGAC AATGGTGGCT GCCAACATGA ATGCATTAAC 
ACCATCGGAA GCTACGTTTG TCAGTGCCGC AATGGCTTTG TTCTTCATGA GAATAAACAC 
GACTGCAAGG AAGCTGAGTG TGAACACAGA ATACACAGCC CAAATGGAGT TATCACAAGC 
CCCAACTGGC CAGACAAGTA TCCGAGTCGC AAGGAATGTA CTTGGGAGAT CAGTGCAACT 
CCCGGACACA GAGTAAAACT TTCTTTCAGC GAATTTGAGA TTGAGCAGCA CCAGGAATGT 
GCTTACGACC ATTTGGAAGT GTTTGATGGA GAAACAGAAA AATCGTCAAT TTTAGGACGC 
CTATGTGGGA GTAAGATCCC AGAGCCTCTG GTTGCCACAG GAAATAAAAT GTTTCTCCGG 
TTTGTGTCTG ATGCATCAGT ACAACGGAAA GGCTTTCAAG CTACCCATTC CACAGAGTGC 
GGCGGCAGGC TGAAAGCAGA AGCAAAGTCG AGAGACCTGT TCTCCCATGC CCAGTTTGGT 
GACAACAATT ACCCAGTGCA AGCGGATTGC GAGTGGATGT TAGCGACAGA GAGGGGTCTG 
CGCATTGAGC TCAGCTTTCA GACATTTGAA GTGGAGGAAG AAGCTGACTG TGGCTATGAC 
TTTATGGAGC TGTTTGATGG GCATGATGCG TCAGCCATGA GACTTGGGCG TTTCTGTGGA 
TCAGGGCCTC CTGAAGAAAT CATATCCACT GGCGATGCCA TATTAATTCA CTTTCACACC 
GATGACACAA TCAGCAAGAA AGGATTTCAT ATACGATACA AAAGCGTGAA ATATCAAGAC 
ATTCTACACA CCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751324.1
CDS	674..3691
Translation	MNMPSWLIFLVTGWTFCDKLFVCSGLDYDYPIYDNEEEKNEAIDYKDPCKAVVFWGDIALDEEDLKMFHIDRTIDLTQHSNEKLGHTTGGLEEHDLSKRRGALYQLIERIRRFGSENATANSQKADTTQSWKSKKIRTPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFIERTDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDDHVTIIRENIQPGQEYNFLKMEPGEVNSLGESYDFDSIMHYARNTFSRGMFLDTILPSRDENGLRPPIGQRTRLSKGDIAQARKLYRCPACGETLQESTGNFSSPGFPNGYPSYTHCIWRISVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWKKSPLLGRFCGDKLPEVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIHKDAGQIQSPNYPDDYRPLKECVWKITVAENYNVGLTFQAFEIERHDSCAYDYLEVRDGSSENSPLIGHFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFLKEEDECARPDNGGCEQRCVNTLGSYKCSCDPGYELGPDKKSCEAACGGLLTKLNGTITTPAWPKEYPPNKNCVWQVVAPSQYRISMKFDYFELEGNEVCKYDYVEVRSGLSSDSKLHGKFCGTEVPEVITSQYNNMRIEFKSDNTVSKKGFRAHFFSDKDECSKDNGGCQHECINTIGSYVCQCRNGFVLHENKHDCKEAECEHRIHSPNGVITSPNWPDKYPSRKECTWEISATPGHRVKLSFSEFEIEQHQECAYDHLEVFDGETEKSSILGRLCGSKIPEPLVATGNKMFLRFVSDASVQRKGFQATHSTECGGRLKAEAKSRDLFSHAQFGDNNYPVQADCEWMLATERGLRIELSFQTFEVEEEADCGYDFMELFDGHDASAMRLGRFCGSGPPEEIISTGDAILIHFHTDDTISKKGFHIRYKSVKYQDILHTK

Target ORF information:

RefSeq Version	XM_031895464.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tolloid like 1 (LOC100495742), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895464.1

ORF Insert Sequence:

ATGAACATGC CTTCTTGGCT CATTTTCCTC GTTACTGGCT GGACTTTCTG TGACAAGTTA 
TTTGTGTGCA GTGGCCTTGA CTATGATTAT CCTATTTATG ACAACGAAGA GGAAAAAAAT 
GAGGCCATCG ATTACAAAGA TCCTTGCAAA GCCGTTGTCT TTTGGGGAGA TATTGCCTTA 
GATGAAGAAG ATTTAAAAAT GTTTCACATA GACAGGACGA TTGACCTGAC ACAACATTCG 
AATGAAAAGC TGGGACACAC CACAGGTGGC CTTGAAGAAC ATGACCTATC AAAAAGGAGA 
GGAGCCCTCT ATCAGCTTAT TGAGAGGATA AGAAGATTTG GCTCTGAAAA TGCAACAGCA 
AATTCACAAA AAGCAGATAC TACACAGTCT TGGAAGAGTA AGAAAATTAG AACACCTAGA 
GCAGCTACGT CACGAACAGA GAGAATATGG CCCGGAGGAG TTATACCGTA TGTCATTGGG 
GGCAATTTCA CTGGCAGCCA GAGAGCAATG TTTAAGCAGG CCATGAGGCA TTGGGAAAAA 
CATACCTGTG TAACATTCAT AGAACGAACA GATGAAGAGA GCTACATTGT GTTTACTTAC 
AGGCCTTGTG GTTGCTGTTC ATATGTGGGA AGAAGAGGAA ATGGCCCCCA AGCAATATCA 
ATTGGCAAAA ACTGCGATAA ATTTGGAATA GTTGTCCATG AGCTAGGCCA CGTGATTGGC 
TTTTGGCATG AACACACTCG ACCAGACAGA GATGACCATG TGACCATAAT CAGAGAAAAC 
ATTCAACCAG GACAAGAATA CAATTTTCTG AAAATGGAAC CAGGGGAAGT GAATTCACTG 
GGAGAATCAT ATGATTTTGA CAGCATCATG CATTATGCAA GGAACACCTT CTCAAGGGGT 
ATGTTTTTGG ATACAATTCT TCCTTCCCGA GATGAAAATG GTTTAAGGCC CCCTATTGGT 
CAACGAACCC GACTAAGCAA AGGAGATATT GCACAAGCAA GAAAACTCTA CAGATGTCCA 
GCTTGTGGAG AAACTTTGCA AGAATCTACA GGGAATTTTT CATCACCTGG GTTTCCAAAT 
GGTTACCCTT CCTATACTCA CTGCATATGG AGGATTTCTG TAACTCCCGG AGAAAAGATT 
GTACTAAACT TCACCACAAT GGACCTATAC AAAAGCAGTC TGTGCTGGTA TGACTATATA 
GAAGTAAGAG ATGGATACTG GAAAAAATCG CCTCTACTTG GTAGGTTCTG TGGAGACAAA 
CTCCCCGAAG TTCTTACAAG CACAGACAGC AGAATGTGGA TAGAGTTCCG CAGCAGCAGC 
AACTGGGTGG GGAAAGGCTT TGCAGCTGTG TATGAAGCAA TATGTGGAGG TGAGATCCAT 
AAAGACGCTG GCCAGATCCA GTCTCCTAAT TATCCAGATG ATTACCGGCC GCTGAAGGAA 
TGCGTGTGGA AAATAACAGT GGCAGAAAAT TACAATGTTG GGTTAACGTT TCAGGCATTT 
GAGATAGAGC GCCATGACAG TTGTGCTTAT GATTACCTGG AAGTGAGAGA TGGAAGCAGT 
GAAAACAGTC CTCTGATTGG CCATTTTTGT GGTTATGATA AACCAGAGGA CATTCGATCT 
ACATCTAATA CCCTATGGAT GAAATTTGTT TCTGATGGGA CAGTAAACAA AGCAGGGTTT 
GCTGCTAACT TTCTAAAAGA GGAAGATGAA TGCGCTAGGC CAGACAATGG AGGATGTGAG 
CAGCGCTGTG TGAACACCCT GGGAAGTTAT AAGTGCTCTT GTGATCCTGG CTATGAGCTT 
GGACCAGACA AAAAAAGCTG TGAAGCTGCT TGTGGAGGTC TTTTAACGAA ACTCAATGGA 
ACAATCACTA CACCAGCTTG GCCAAAAGAA TACCCTCCAA ACAAAAACTG TGTGTGGCAG 
GTTGTGGCTC CCTCTCAGTA CAGAATCTCT ATGAAATTTG ATTATTTTGA GTTAGAAGGC 
AATGAGGTGT GCAAGTATGA CTATGTTGAG GTTCGAAGTG GCCTCTCCTC TGATTCCAAG 
CTACATGGGA AATTTTGTGG GACAGAGGTA CCTGAAGTAA TAACATCTCA GTATAACAAC 
ATGAGGATTG AATTTAAGTC TGACAACACA GTATCAAAAA AGGGCTTTCG GGCACACTTC 
TTTTCAGATA AAGATGAGTG CTCAAAAGAC AATGGTGGCT GCCAACATGA ATGCATTAAC 
ACCATCGGAA GCTACGTTTG TCAGTGCCGC AATGGCTTTG TTCTTCATGA GAATAAACAC 
GACTGCAAGG AAGCTGAGTG TGAACACAGA ATACACAGCC CAAATGGAGT TATCACAAGC 
CCCAACTGGC CAGACAAGTA TCCGAGTCGC AAGGAATGTA CTTGGGAGAT CAGTGCAACT 
CCCGGACACA GAGTAAAACT TTCTTTCAGC GAATTTGAGA TTGAGCAGCA CCAGGAATGT 
GCTTACGACC ATTTGGAAGT GTTTGATGGA GAAACAGAAA AATCGTCAAT TTTAGGACGC 
CTATGTGGGA GTAAGATCCC AGAGCCTCTG GTTGCCACAG GAAATAAAAT GTTTCTCCGG 
TTTGTGTCTG ATGCATCAGT ACAACGGAAA GGCTTTCAAG CTACCCATTC CACAGAGTGC 
GGCGGCAGGC TGAAAGCAGA AGCAAAGTCG AGAGACCTGT TCTCCCATGC CCAGTTTGGT 
GACAACAATT ACCCAGTGCA AGCGGATTGC GAGTGGATGT TAGCGACAGA GAGGGGTCTG 
CGCATTGAGC TCAGCTTTCA GACATTTGAA GTGGAGGAAG AAGCTGACTG TGGCTATGAC 
TTTATGGAGC TGTTTGATGG GCATGATGCG TCAGCCATGA GACTTGGGCG TTTCTGTGGA 
TCAGGGCCTC CTGAAGAAAT CATATCCACT GGCGATGCCA TATTAATTCA CTTTCACACC 
GATGACACAA TCAGCAAGAA AGGATTTCAT ATACGATACA AAAGCGTGAA ATATCAAGAC 
ATTCTACACA CCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100495742 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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