Tll1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tll1
Entrez Gene ID	21892
Full Name	tolloid-like
Synonyms	Tll,Tll-1,b2b2476Clo
General protein information	Preferred Names tolloid-like Names tolloid-like protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 B3.1\|8 32.19 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011242185.3	XP_011240487.1	tolloid-like protein 1 isoform X2
XM_006509315.3	XP_006509378.1	tolloid-like protein 1 isoform X3
XM_006509314.3	XP_006509377.1	tolloid-like protein 1 isoform X1
NM_009390.2	NP_033416.2	tolloid-like protein 1 precursor
XM_006509314.4	XP_006509377.1	tolloid-like protein 1 isoform X1
XM_011242185.2	XP_011240487.1	tolloid-like protein 1 isoform X2
NM_009390.3	NP_033416.2	tolloid-like protein 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TLL1	NP_036596.3
	TLL1	XP_001101883.2
Gallus gallus (chicken)	TLL1	NP_990034.1
Danio rerio (zebrafish)	tll1	NP_571085.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003702	XP_313494.5
Caenorhabditis elegans (roundworm)	nas-39	NP_510672.2
Drosophila melanogaster (fruit fly)	tok	NP_733035.1
Bos taurus (cattle)	TLL1	NP_001180043.1
Mus musculus (house mouse)	Tll1	NP_033416.2
Canis lupus familiaris (dog)	TLL1	XP_539791.2
Pan troglodytes (chimpanzee)	TLL1	XP_003310613.1
Rattus norvegicus (Norway rat)	Tll1	NP_001099551.1
Xenopus tropicalis (tropical clawed frog)	LOC100495742	XP_004911222.1

Gene Ontology
Bibliography

Related articles in PubMed

Inactivation of bone morphogenetic protein 1 (Bmp1) and tolloid-like 1 (Tll1) in cells expressing type I collagen leads to dental and periodontal defects in mice.
Zhang H, Jani P, Liang T, Lu Y, Qin C
Journal of molecular histology48(2)83-98(2017 Apr)

Essential Roles of Bone Morphogenetic Protein-1 and Mammalian Tolloid-like 1 in Postnatal Root Dentin Formation.
Wang J, Muir AM, Ren Y, Massoudi D, Greenspan DS, Feng JQ
Journal of endodontics43(1)109-115(2017 Jan)

BMP1-like proteinases are essential to the structure and wound healing of skin.
Muir AM, Massoudi D, Nguyen N, Keene DR, Lee SJ, Birk DE, Davidson JM, Marinkovich MP, Greenspan DS
Matrix biology : journal of the International Society for Matrix Biology56114-131(2016 Dec)

Mammalian tolloid proteinases: role in growth factor signalling.
Troilo H, Bayley CP, Barrett AL, Lockhart-Cairns MP, Jowitt TA, Baldock C
FEBS letters590(15)2398-407(2016 08)

Gene network and familial analyses uncover a gene network involving Tbx5/Osr1/Pcsk6 interaction in the second heart field for atrial septation.
Zhang KK, Xiang M, Zhou L, Liu J, Curry N, Heine Su?er D, Garcia-Pavia P, Zhang X, Wang Q, Xie L
Human molecular genetics25(6)1140-51(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the proteinases encoded by Bmp1 and Tll1 genes play essential roles in the development and maintenance of mouse dentin and periodontal ligaments.
Title: Inactivation of bone morphogenetic protein 1 (Bmp1) and tolloid-like 1 (Tll1) in cells expressing type I collagen leads to dental and periodontal defects in mice.

plays crucial role in maintaining extracellular matrix homeostasis essential to root formation and dentin mineralization
Title: Essential Roles of Bone Morphogenetic Protein-1 and Mammalian Tolloid-like 1 in Postnatal Root Dentin Formation.

The presented data demonstrate Bmp1/Tll1 products to be of profound importance to bone growth and modeling.
Title: Induced ablation of Bmp1 and Tll1 produces osteogenesis imperfecta in mice.

TLL1 mRNA levels significantly increased in the tibialis anterior muscle with 3 days of hindlimb suspension and significantly decreased in response to 2 days of food deprivation.
Title: Skeletal muscle expression of bone morphogenetic protein-1 and tolloid-like-1 extracellular proteases in different fiber types and in response to unloading, food deprivation and differentiation.

The results show that Nkx2-5 is a direct activator of Tll-1 expression and provide insight into the mechanism of the defects found in both the Tll-1 and Nkx2-5 knockout mice.
Title: Activation of Tolloid-like 1 gene expression by the cardiac specific homeobox gene Nkx2-5.

The following Tll1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tll1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu40770	XM_006509315.3 Latest version!	Mus musculus tolloid-like (Tll1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu40769	XM_006509314.4 Latest version!	Mus musculus tolloid-like (Tll1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu68921	XM_011242185.3 Latest version!	Mus musculus tolloid-like (Tll1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu04058	NM_009390.3 Latest version!	Mus musculus tolloid-like (Tll1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$594.30 $849.00
View Old Accession Versions >>
OMu40769	XM_006509314.3	Mus musculus tolloid-like (Tll1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu68921	XM_011242185.2	Mus musculus tolloid-like (Tll1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu04058	NM_009390.2	Mus musculus tolloid-like (Tll1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$594.30 $849.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tll1 ( XM_006509315.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509378.1 Mus musculus tolloid-like (Tll1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu40770
Clone ID Related Accession (Same CDS sequence)	XM_006509315.3
Accession Version	XM_006509315.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tolloid-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509315.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTT ACAAAAGTAG TTTGTGCTGG TATGATTACA TTGAAGTAAG AGATGGTTAC 
TGGAGGAAGT CACCTCTCCT TGGTAGATTC TGTGGGGACA AAGTGGCTGG AGTTCTTACA 
TCTACGGACA GCAGAATGTG GATTGAGTTT CGTAGCAGCA GTAACTGGGT AGGAAAAGGG 
TTTGCAGCTG TCTATGAAGC GATTTGTGGA GGGGAGATAA GGAAAAACGA AGGGCAGATT 
CAGTCTCCCA ATTACCCCGA TGACTACCGA CCAATGAAGG AGTGTGTATG GAAAATAATG 
GTGTCCGAGG GCTACCATGT TGGACTGACC TTTCAGGCCT TTGAGATCGA AAGACATGAC 
AGCTGTGCCT ATGACCACCT AGAAGTTCGA GATGGAGCCA GTGAGAACAG CCCTTTGATA 
GGACGGTTCT GTGGTTATGA CAAACCTGAA GATATAAGGT CTACTTCCAA CACCCTGTGG 
ATGAAGTTTG TCTCTGACGG GACTGTGAAC AAGGCAGGGT TTGCTGCGAA CTTTTTTAAA 
GAGGAAGATG AGTGTGCCAA ACCTGACCGA GGAGGCTGTG AACAGAGGTG TCTTAACACA 
CTAGGCAGCT ACCAGTGTGC CTGTGAGCCT GGCTATGAAC TGGGGCCAGA CAGAAGAAGC 
TGTGAAGCTG CTTGCGGAGG ACTTCTGACG AAGCTCAATG GCACCATAAC CACCCCCGGC 
TGGCCCAAAG AGTACCCTCC AAACAAAAAC TGTGTGTGGC AAGTGATCGC GCCAAGCCAG 
TACAGAATCT CTGTGAAGTT TGAGTTTTTT GAATTGGAAG GCAATGAAGT TTGCAAATAC 
GATTACGTGG AGATCTGGAG CGGCCTTTCC TCTGAGTCTA AACTGCATGG CAAGTTCTGT 
GGCGCTGACA TACCTGAAGT GATGACTTCC CATTTCAACA ACATGAGGAT TGAATTCAAG 
TCAGACAACA CTGTATCCAA GAAGGGCTTC AAAGCACATT TTTTCTCAGA TAAGGATGAG 
TGTTCAAAGG ATAATGGTGG CTGTCAGCAT GAGTGTGTCA ACACGATGGG AAGTTACACG 
TGTCAGTGCC GGAATGGATT CGTGTTGCAT GAGAACAAGC ATGATTGCAA GGAAGCCGAG 
TGTGAACAGA AGATACACAG CCCAAGTGGT CTCATCACCA GTCCCAACTG GCCAGACAAG 
TATCCAAGCA GGAAAGAGTG CACGTGGGTG ATCAGTGCCA TTCCTGGCCA CCGCATCACA 
TTAGCCTTCA ATGAGTTTGA GGTTGAACAA CATCAAGAAT GTGCTTATGA TCACTTGGAA 
ATTTTTGATG GAGAAACGGA GAAGTCACCA ATACTTGGCC GACTATGTGG CAGCAAGATA 
CCAGATCCCC TCATGGCTAC TGGGAATGAA ATGTTTATTC GGTTTATTTC TGATGCCTCT 
GTTCAAAGAA AAGGCTTTCA AGCTACACAT TCCACAGAGT GTGGTGGTCG ATTGAAAGCA 
GAGTCAAAGC CTAGAGACCT GTACTCCCAT GCTCAGTTTG GTGATAATAA CTACCCAGGA 
CAACTGGACT GTGAATGGTT GTTGGTGTCA GAACGAGGAT CTCGACTTGA ATTGTCCTTC 
CAGACATTCG AAGTAGAAGA AGAAGCTGAC TGTGGCTATG ACTATGTTGA AGTCTTTGAT 
GGTCTCAGTT CAAAAGCTGT GGGTCTTGGT AGATTCTGTG GGTCAGGGCC ACCAGAAGAA 
ATCTATTCAA TTGGAGATGT GGCTTTGATT CATTTCCACA CAGATGACAC TATCAACAAG 
AAAGGATTTT ATATAAGATA TAAAAGTATA AGATACCCGG AAACGATGCA TGCCAAGAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509378.1
CDS	209..2071
Translation	MDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKVAGVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKIMVSEGYHVGLTFQAFEIERHDSCAYDHLEVRDGASENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVIAPSQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGADIPEVMTSHFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYTCQCRNGFVLHENKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWVISAIPGHRITLAFNEFEVEQHQECAYDHLEIFDGETEKSPILGRLCGSKIPDPLMATGNEMFIRFISDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQLDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVEVFDGLSSKAVGLGRFCGSGPPEEIYSIGDVALIHFHTDDTINKKGFYIRYKSIRYPETMHAKN

Target ORF information:

RefSeq Version	XM_006509315.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tolloid-like (Tll1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509315.3

ORF Insert Sequence:

ATGGACCTTT ACAAAAGTAG TTTGTGCTGG TATGATTACA TTGAAGTAAG AGATGGTTAC 
TGGAGGAAGT CACCTCTCCT TGGTAGATTC TGTGGGGACA AAGTGGCTGG AGTTCTTACA 
TCTACGGACA GCAGAATGTG GATTGAGTTT CGTAGCAGCA GTAACTGGGT AGGAAAAGGG 
TTTGCAGCTG TCTATGAAGC GATTTGTGGA GGGGAGATAA GGAAAAACGA AGGGCAGATT 
CAGTCTCCCA ATTACCCCGA TGACTACCGA CCAATGAAGG AGTGTGTATG GAAAATAATG 
GTGTCCGAGG GCTACCATGT TGGACTGACC TTTCAGGCCT TTGAGATCGA AAGACATGAC 
AGCTGTGCCT ATGACCACCT AGAAGTTCGA GATGGAGCCA GTGAGAACAG CCCTTTGATA 
GGACGGTTCT GTGGTTATGA CAAACCTGAA GATATAAGGT CTACTTCCAA CACCCTGTGG 
ATGAAGTTTG TCTCTGACGG GACTGTGAAC AAGGCAGGGT TTGCTGCGAA CTTTTTTAAA 
GAGGAAGATG AGTGTGCCAA ACCTGACCGA GGAGGCTGTG AACAGAGGTG TCTTAACACA 
CTAGGCAGCT ACCAGTGTGC CTGTGAGCCT GGCTATGAAC TGGGGCCAGA CAGAAGAAGC 
TGTGAAGCTG CTTGCGGAGG ACTTCTGACG AAGCTCAATG GCACCATAAC CACCCCCGGC 
TGGCCCAAAG AGTACCCTCC AAACAAAAAC TGTGTGTGGC AAGTGATCGC GCCAAGCCAG 
TACAGAATCT CTGTGAAGTT TGAGTTTTTT GAATTGGAAG GCAATGAAGT TTGCAAATAC 
GATTACGTGG AGATCTGGAG CGGCCTTTCC TCTGAGTCTA AACTGCATGG CAAGTTCTGT 
GGCGCTGACA TACCTGAAGT GATGACTTCC CATTTCAACA ACATGAGGAT TGAATTCAAG 
TCAGACAACA CTGTATCCAA GAAGGGCTTC AAAGCACATT TTTTCTCAGA TAAGGATGAG 
TGTTCAAAGG ATAATGGTGG CTGTCAGCAT GAGTGTGTCA ACACGATGGG AAGTTACACG 
TGTCAGTGCC GGAATGGATT CGTGTTGCAT GAGAACAAGC ATGATTGCAA GGAAGCCGAG 
TGTGAACAGA AGATACACAG CCCAAGTGGT CTCATCACCA GTCCCAACTG GCCAGACAAG 
TATCCAAGCA GGAAAGAGTG CACGTGGGTG ATCAGTGCCA TTCCTGGCCA CCGCATCACA 
TTAGCCTTCA ATGAGTTTGA GGTTGAACAA CATCAAGAAT GTGCTTATGA TCACTTGGAA 
ATTTTTGATG GAGAAACGGA GAAGTCACCA ATACTTGGCC GACTATGTGG CAGCAAGATA 
CCAGATCCCC TCATGGCTAC TGGGAATGAA ATGTTTATTC GGTTTATTTC TGATGCCTCT 
GTTCAAAGAA AAGGCTTTCA AGCTACACAT TCCACAGAGT GTGGTGGTCG ATTGAAAGCA 
GAGTCAAAGC CTAGAGACCT GTACTCCCAT GCTCAGTTTG GTGATAATAA CTACCCAGGA 
CAACTGGACT GTGAATGGTT GTTGGTGTCA GAACGAGGAT CTCGACTTGA ATTGTCCTTC 
CAGACATTCG AAGTAGAAGA AGAAGCTGAC TGTGGCTATG ACTATGTTGA AGTCTTTGAT 
GGTCTCAGTT CAAAAGCTGT GGGTCTTGGT AGATTCTGTG GGTCAGGGCC ACCAGAAGAA 
ATCTATTCAA TTGGAGATGT GGCTTTGATT CATTTCCACA CAGATGACAC TATCAACAAG 
AAAGGATTTT ATATAAGATA TAAAAGTATA AGATACCCGG AAACGATGCA TGCCAAGAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tll1 ( XM_006509314.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509377.1 Mus musculus tolloid-like (Tll1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu40769
Clone ID Related Accession (Same CDS sequence)	XM_006509314.3 , XM_006509314.4
Accession Version	XM_006509314.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tolloid-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509314.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAG GTCAGAGGCA ATCTGTGTTT TGGGGTGACA TCGCCTTAGA TGATGAAGAC 
TTAAATATCT TCCAAATAGA CAGGACAATT GACCTGACCC AGAGCCCCTT TGGAAAACTT 
GGACATATTA CAGGTGGCTT TGGAGACCAT GGCATGCCAA AGAAGCGAGG GGCACTCTAC 
CAACTTATAG AGAGGATCAG AAGAATTGGC TCTGGCTTGG AGCAAAATAA CACGATGAAG 
GGAAAAGCAC CTCCAAAATT GTCAGAGCAA AGTGAGAAAA ATCGAGTTCC CAGAGCTGCT 
ACCTCAAGAA CGGAAAGGAT ATGGCCTGGG GGTGTCATTC CTTATGTCAT AGGAGGAAAC 
TTTACTGGCA GCCAGAGAGC CATGTTCAAG CAGGCCATGA GACACTGGGA AAAGCACACC 
TGTGTGACGT TCACTGAGAG AAGTGATGAA GAAAGTTATA TTGTGTTCAC CTACAGGCCT 
TGTGGATGCT GCTCCTATGT TGGTCGGCGG GGAAATGGCC CTCAGGCCAT CTCTATTGGC 
AAGAACTGTG ACAAGTTTGG AATTGTTGTT CATGAACTGG GCCACGTGAT AGGCTTCTGG 
CATGAACATA CCCGCCCAGA CCGAGACAAC CATGTCACCA TCATTAGAGA GAACATCCAG 
CCAGGTCAAG AGTACAATTT TCTAAAGATG GAGCCTGGAG AAGTGAACTC TCTTGGGGAA 
AGATATGATT TTGACAGTAT CATGCACTAC GCCAGGAACA CCTTCTCAAG AGGGATGTTT 
TTAGACACAA TACTCCCCTC CCGTGATGAT AATGGCATTC GTCCTGCAAT TGGTCAACGG 
ACCCGGTTAA GCAAAGGAGA CATTGCACAA GCAAGAAAGC TGTATCGATG CCCAGCATGT 
GGAGAAACCC TGCAAGAATC CAGTGGCAAC CTTTCTTCCC CAGGATTCCC AAATGGCTAC 
CCTTCCTACA CACACTGCAT CTGGAGAGTG TCTGTGACCC CGGGAGAAAA GATTGTCTTG 
AATTTTACCA CAATGGACCT TTACAAAAGT AGTTTGTGCT GGTATGATTA CATTGAAGTA 
AGAGATGGTT ACTGGAGGAA GTCACCTCTC CTTGGTAGAT TCTGTGGGGA CAAAGTGGCT 
GGAGTTCTTA CATCTACGGA CAGCAGAATG TGGATTGAGT TTCGTAGCAG CAGTAACTGG 
GTAGGAAAAG GGTTTGCAGC TGTCTATGAA GCGATTTGTG GAGGGGAGAT AAGGAAAAAC 
GAAGGGCAGA TTCAGTCTCC CAATTACCCC GATGACTACC GACCAATGAA GGAGTGTGTA 
TGGAAAATAA TGGTGTCCGA GGGCTACCAT GTTGGACTGA CCTTTCAGGC CTTTGAGATC 
GAAAGACATG ACAGCTGTGC CTATGACCAC CTAGAAGTTC GAGATGGAGC CAGTGAGAAC 
AGCCCTTTGA TAGGACGGTT CTGTGGTTAT GACAAACCTG AAGATATAAG GTCTACTTCC 
AACACCCTGT GGATGAAGTT TGTCTCTGAC GGGACTGTGA ACAAGGCAGG GTTTGCTGCG 
AACTTTTTTA AAGAGGAAGA TGAGTGTGCC AAACCTGACC GAGGAGGCTG TGAACAGAGG 
TGTCTTAACA CACTAGGCAG CTACCAGTGT GCCTGTGAGC CTGGCTATGA ACTGGGGCCA 
GACAGAAGAA GCTGTGAAGC TGCTTGCGGA GGACTTCTGA CGAAGCTCAA TGGCACCATA 
ACCACCCCCG GCTGGCCCAA AGAGTACCCT CCAAACAAAA ACTGTGTGTG GCAAGTGATC 
GCGCCAAGCC AGTACAGAAT CTCTGTGAAG TTTGAGTTTT TTGAATTGGA AGGCAATGAA 
GTTTGCAAAT ACGATTACGT GGAGATCTGG AGCGGCCTTT CCTCTGAGTC TAAACTGCAT 
GGCAAGTTCT GTGGCGCTGA CATACCTGAA GTGATGACTT CCCATTTCAA CAACATGAGG 
ATTGAATTCA AGTCAGACAA CACTGTATCC AAGAAGGGCT TCAAAGCACA TTTTTTCTCA 
GATAAGGATG AGTGTTCAAA GGATAATGGT GGCTGTCAGC ATGAGTGTGT CAACACGATG 
GGAAGTTACA CGTGTCAGTG CCGGAATGGA TTCGTGTTGC ATGAGAACAA GCATGATTGC 
AAGGAAGCCG AGTGTGAACA GAAGATACAC AGCCCAAGTG GTCTCATCAC CAGTCCCAAC 
TGGCCAGACA AGTATCCAAG CAGGAAAGAG TGCACGTGGG TGATCAGTGC CATTCCTGGC 
CACCGCATCA CATTAGCCTT CAATGAGTTT GAGGTTGAAC AACATCAAGA ATGTGCTTAT 
GATCACTTGG AAATTTTTGA TGGAGAAACG GAGAAGTCAC CAATACTTGG CCGACTATGT 
GGCAGCAAGA TACCAGATCC CCTCATGGCT ACTGGGAATG AAATGTTTAT TCGGTTTATT 
TCTGATGCCT CTGTTCAAAG AAAAGGCTTT CAAGCTACAC ATTCCACAGA GTGTGGTGGT 
CGATTGAAAG CAGAGTCAAA GCCTAGAGAC CTGTACTCCC ATGCTCAGTT TGGTGATAAT 
AACTACCCAG GACAACTGGA CTGTGAATGG TTGTTGGTGT CAGAACGAGG ATCTCGACTT 
GAATTGTCCT TCCAGACATT CGAAGTAGAA GAAGAAGCTG ACTGTGGCTA TGACTATGTT 
GAAGTCTTTG ATGGTCTCAG TTCAAAAGCT GTGGGTCTTG GTAGATTCTG TGGGTCAGGG 
CCACCAGAAG AAATCTATTC AATTGGAGAT GTGGCTTTGA TTCATTTCCA CACAGATGAC 
ACTATCAACA AGAAAGGATT TTATATAAGA TATAAAAGTA TAAGATACCC GGAAACGATG 
CATGCCAAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509377.1
CDS	169..3063
Translation	MMGGQRQSVFWGDIALDDEDLNIFQIDRTIDLTQSPFGKLGHITGGFGDHGMPKKRGALYQLIERIRRIGSGLEQNNTMKGKAPPKLSEQSEKNRVPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFTERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESSGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKVAGVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKIMVSEGYHVGLTFQAFEIERHDSCAYDHLEVRDGASENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVIAPSQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGADIPEVMTSHFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYTCQCRNGFVLHENKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWVISAIPGHRITLAFNEFEVEQHQECAYDHLEIFDGETEKSPILGRLCGSKIPDPLMATGNEMFIRFISDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQLDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVEVFDGLSSKAVGLGRFCGSGPPEEIYSIGDVALIHFHTDDTINKKGFYIRYKSIRYPETMHAKN

Target ORF information:

RefSeq Version	XM_006509314.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tolloid-like (Tll1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509314.3

ORF Insert Sequence:

ATGATGGGAG GTCAGAGGCA ATCTGTGTTT TGGGGTGACA TCGCCTTAGA TGATGAAGAC 
TTAAATATCT TCCAAATAGA CAGGACAATT GACCTGACCC AGAGCCCCTT TGGAAAACTT 
GGACATATTA CAGGTGGCTT TGGAGACCAT GGCATGCCAA AGAAGCGAGG GGCACTCTAC 
CAACTTATAG AGAGGATCAG AAGAATTGGC TCTGGCTTGG AGCAAAATAA CACGATGAAG 
GGAAAAGCAC CTCCAAAATT GTCAGAGCAA AGTGAGAAAA ATCGAGTTCC CAGAGCTGCT 
ACCTCAAGAA CGGAAAGGAT ATGGCCTGGG GGTGTCATTC CTTATGTCAT AGGAGGAAAC 
TTTACTGGCA GCCAGAGAGC CATGTTCAAG CAGGCCATGA GACACTGGGA AAAGCACACC 
TGTGTGACGT TCACTGAGAG AAGTGATGAA GAAAGTTATA TTGTGTTCAC CTACAGGCCT 
TGTGGATGCT GCTCCTATGT TGGTCGGCGG GGAAATGGCC CTCAGGCCAT CTCTATTGGC 
AAGAACTGTG ACAAGTTTGG AATTGTTGTT CATGAACTGG GCCACGTGAT AGGCTTCTGG 
CATGAACATA CCCGCCCAGA CCGAGACAAC CATGTCACCA TCATTAGAGA GAACATCCAG 
CCAGGTCAAG AGTACAATTT TCTAAAGATG GAGCCTGGAG AAGTGAACTC TCTTGGGGAA 
AGATATGATT TTGACAGTAT CATGCACTAC GCCAGGAACA CCTTCTCAAG AGGGATGTTT 
TTAGACACAA TACTCCCCTC CCGTGATGAT AATGGCATTC GTCCTGCAAT TGGTCAACGG 
ACCCGGTTAA GCAAAGGAGA CATTGCACAA GCAAGAAAGC TGTATCGATG CCCAGCATGT 
GGAGAAACCC TGCAAGAATC CAGTGGCAAC CTTTCTTCCC CAGGATTCCC AAATGGCTAC 
CCTTCCTACA CACACTGCAT CTGGAGAGTG TCTGTGACCC CGGGAGAAAA GATTGTCTTG 
AATTTTACCA CAATGGACCT TTACAAAAGT AGTTTGTGCT GGTATGATTA CATTGAAGTA 
AGAGATGGTT ACTGGAGGAA GTCACCTCTC CTTGGTAGAT TCTGTGGGGA CAAAGTGGCT 
GGAGTTCTTA CATCTACGGA CAGCAGAATG TGGATTGAGT TTCGTAGCAG CAGTAACTGG 
GTAGGAAAAG GGTTTGCAGC TGTCTATGAA GCGATTTGTG GAGGGGAGAT AAGGAAAAAC 
GAAGGGCAGA TTCAGTCTCC CAATTACCCC GATGACTACC GACCAATGAA GGAGTGTGTA 
TGGAAAATAA TGGTGTCCGA GGGCTACCAT GTTGGACTGA CCTTTCAGGC CTTTGAGATC 
GAAAGACATG ACAGCTGTGC CTATGACCAC CTAGAAGTTC GAGATGGAGC CAGTGAGAAC 
AGCCCTTTGA TAGGACGGTT CTGTGGTTAT GACAAACCTG AAGATATAAG GTCTACTTCC 
AACACCCTGT GGATGAAGTT TGTCTCTGAC GGGACTGTGA ACAAGGCAGG GTTTGCTGCG 
AACTTTTTTA AAGAGGAAGA TGAGTGTGCC AAACCTGACC GAGGAGGCTG TGAACAGAGG 
TGTCTTAACA CACTAGGCAG CTACCAGTGT GCCTGTGAGC CTGGCTATGA ACTGGGGCCA 
GACAGAAGAA GCTGTGAAGC TGCTTGCGGA GGACTTCTGA CGAAGCTCAA TGGCACCATA 
ACCACCCCCG GCTGGCCCAA AGAGTACCCT CCAAACAAAA ACTGTGTGTG GCAAGTGATC 
GCGCCAAGCC AGTACAGAAT CTCTGTGAAG TTTGAGTTTT TTGAATTGGA AGGCAATGAA 
GTTTGCAAAT ACGATTACGT GGAGATCTGG AGCGGCCTTT CCTCTGAGTC TAAACTGCAT 
GGCAAGTTCT GTGGCGCTGA CATACCTGAA GTGATGACTT CCCATTTCAA CAACATGAGG 
ATTGAATTCA AGTCAGACAA CACTGTATCC AAGAAGGGCT TCAAAGCACA TTTTTTCTCA 
GATAAGGATG AGTGTTCAAA GGATAATGGT GGCTGTCAGC ATGAGTGTGT CAACACGATG 
GGAAGTTACA CGTGTCAGTG CCGGAATGGA TTCGTGTTGC ATGAGAACAA GCATGATTGC 
AAGGAAGCCG AGTGTGAACA GAAGATACAC AGCCCAAGTG GTCTCATCAC CAGTCCCAAC 
TGGCCAGACA AGTATCCAAG CAGGAAAGAG TGCACGTGGG TGATCAGTGC CATTCCTGGC 
CACCGCATCA CATTAGCCTT CAATGAGTTT GAGGTTGAAC AACATCAAGA ATGTGCTTAT 
GATCACTTGG AAATTTTTGA TGGAGAAACG GAGAAGTCAC CAATACTTGG CCGACTATGT 
GGCAGCAAGA TACCAGATCC CCTCATGGCT ACTGGGAATG AAATGTTTAT TCGGTTTATT 
TCTGATGCCT CTGTTCAAAG AAAAGGCTTT CAAGCTACAC ATTCCACAGA GTGTGGTGGT 
CGATTGAAAG CAGAGTCAAA GCCTAGAGAC CTGTACTCCC ATGCTCAGTT TGGTGATAAT 
AACTACCCAG GACAACTGGA CTGTGAATGG TTGTTGGTGT CAGAACGAGG ATCTCGACTT 
GAATTGTCCT TCCAGACATT CGAAGTAGAA GAAGAAGCTG ACTGTGGCTA TGACTATGTT 
GAAGTCTTTG ATGGTCTCAG TTCAAAAGCT GTGGGTCTTG GTAGATTCTG TGGGTCAGGG 
CCACCAGAAG AAATCTATTC AATTGGAGAT GTGGCTTTGA TTCATTTCCA CACAGATGAC 
ACTATCAACA AGAAAGGATT TTATATAAGA TATAAAAGTA TAAGATACCC GGAAACGATG 
CATGCCAAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tll1 ( XM_011242185.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240487.1 Mus musculus tolloid-like (Tll1), transcript variant X2, mRNA.

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CloneID	OMu68921
Clone ID Related Accession (Same CDS sequence)	XM_011242185.3 , XM_011242185.2
Accession Version	XM_011242185.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tolloid-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242185.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTGA CAGTATCATG CACTACGCCA GGAACACCTT CTCAAGGTTG GAGTCTCAGA 
GGGATGTTTT TAGACACAAT ACTCCCCTCC CGTGATGATA ATGGCATTCG TCCTGCAATT 
GGTCAACGGA CCCGGTTAAG CAAAGGAGAC ATTGCACAAG CAAGAAAGCT GTATCGATGC 
CCAGCATGTG GAGAAACCCT GCAAGAATCC AGTGGCAACC TTTCTTCCCC AGGATTCCCA 
AATGGCTACC CTTCCTACAC ACACTGCATC TGGAGAGTGT CTGTGACCCC GGGAGAAAAG 
ATTGTCTTGA ATTTTACCAC AATGGACCTT TACAAAAGTA GTTTGTGCTG GTATGATTAC 
ATTGAAGTAA GAGATGGTTA CTGGAGGAAG TCACCTCTCC TTGGTAGATT CTGTGGGGAC 
AAAGTGGCTG GAGTTCTTAC ATCTACGGAC AGCAGAATGT GGATTGAGTT TCGTAGCAGC 
AGTAACTGGG TAGGAAAAGG GTTTGCAGCT GTCTATGAAG CGATTTGTGG AGGGGAGATA 
AGGAAAAACG AAGGGCAGAT TCAGTCTCCC AATTACCCCG ATGACTACCG ACCAATGAAG 
GAGTGTGTAT GGAAAATAAT GGTGTCCGAG GGCTACCATG TTGGACTGAC CTTTCAGGCC 
TTTGAGATCG AAAGACATGA CAGCTGTGCC TATGACCACC TAGAAGTTCG AGATGGAGCC 
AGTGAGAACA GCCCTTTGAT AGGACGGTTC TGTGGTTATG ACAAACCTGA AGATATAAGG 
TCTACTTCCA ACACCCTGTG GATGAAGTTT GTCTCTGACG GGACTGTGAA CAAGGCAGGG 
TTTGCTGCGA ACTTTTTTAA AGAGGAAGAT GAGTGTGCCA AACCTGACCG AGGAGGCTGT 
GAACAGAGGT GTCTTAACAC ACTAGGCAGC TACCAGTGTG CCTGTGAGCC TGGCTATGAA 
CTGGGGCCAG ACAGAAGAAG CTGTGAAGCT GCTTGCGGAG GACTTCTGAC GAAGCTCAAT 
GGCACCATAA CCACCCCCGG CTGGCCCAAA GAGTACCCTC CAAACAAAAA CTGTGTGTGG 
CAAGTGATCG CGCCAAGCCA GTACAGAATC TCTGTGAAGT TTGAGTTTTT TGAATTGGAA 
GGCAATGAAG TTTGCAAATA CGATTACGTG GAGATCTGGA GCGGCCTTTC CTCTGAGTCT 
AAACTGCATG GCAAGTTCTG TGGCGCTGAC ATACCTGAAG TGATGACTTC CCATTTCAAC 
AACATGAGGA TTGAATTCAA GTCAGACAAC ACTGTATCCA AGAAGGGCTT CAAAGCACAT 
TTTTTCTCAG ATAAGGATGA GTGTTCAAAG GATAATGGTG GCTGTCAGCA TGAGTGTGTC 
AACACGATGG GAAGTTACAC GTGTCAGTGC CGGAATGGAT TCGTGTTGCA TGAGAACAAG 
CATGATTGCA AGGAAGCCGA GTGTGAACAG AAGATACACA GCCCAAGTGG TCTCATCACC 
AGTCCCAACT GGCCAGACAA GTATCCAAGC AGGAAAGAGT GCACGTGGGT GATCAGTGCC 
ATTCCTGGCC ACCGCATCAC ATTAGCCTTC AATGAGTTTG AGGTTGAACA ACATCAAGAA 
TGTGCTTATG ATCACTTGGA AATTTTTGAT GGAGAAACGG AGAAGTCACC AATACTTGGC 
CGACTATGTG GCAGCAAGAT ACCAGATCCC CTCATGGCTA CTGGGAATGA AATGTTTATT 
CGGTTTATTT CTGATGCCTC TGTTCAAAGA AAAGGCTTTC AAGCTACACA TTCCACAGAG 
TGTGGTGGTC GATTGAAAGC AGAGTCAAAG CCTAGAGACC TGTACTCCCA TGCTCAGTTT 
GGTGATAATA ACTACCCAGG ACAACTGGAC TGTGAATGGT TGTTGGTGTC AGAACGAGGA 
TCTCGACTTG AATTGTCCTT CCAGACATTC GAAGTAGAAG AAGAAGCTGA CTGTGGCTAT 
GACTATGTTG AAGTCTTTGA TGGTCTCAGT TCAAAAGCTG TGGGTCTTGG TAGATTCTGT 
GGGTCAGGGC CACCAGAAGA AATCTATTCA ATTGGAGATG TGGCTTTGAT TCATTTCCAC 
ACAGATGACA CTATCAACAA GAAAGGATTT TATATAAGAT ATAAAAGTAT AAGATACCCG 
GAAACGATGC ATGCCAAGAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240487.1
CDS	532..2715
Translation	MILTVSCTTPGTPSQGWSLRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESSGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKVAGVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKIMVSEGYHVGLTFQAFEIERHDSCAYDHLEVRDGASENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVIAPSQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGADIPEVMTSHFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYTCQCRNGFVLHENKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWVISAIPGHRITLAFNEFEVEQHQECAYDHLEIFDGETEKSPILGRLCGSKIPDPLMATGNEMFIRFISDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQLDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVEVFDGLSSKAVGLGRFCGSGPPEEIYSIGDVALIHFHTDDTINKKGFYIRYKSIRYPETMHAKN

Target ORF information:

RefSeq Version	XM_011242185.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tolloid-like (Tll1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242185.2

ORF Insert Sequence:

ATGATTTTGA CAGTATCATG CACTACGCCA GGAACACCTT CTCAAGGTTG GAGTCTCAGA 
GGGATGTTTT TAGACACAAT ACTCCCCTCC CGTGATGATA ATGGCATTCG TCCTGCAATT 
GGTCAACGGA CCCGGTTAAG CAAAGGAGAC ATTGCACAAG CAAGAAAGCT GTATCGATGC 
CCAGCATGTG GAGAAACCCT GCAAGAATCC AGTGGCAACC TTTCTTCCCC AGGATTCCCA 
AATGGCTACC CTTCCTACAC ACACTGCATC TGGAGAGTGT CTGTGACCCC GGGAGAAAAG 
ATTGTCTTGA ATTTTACCAC AATGGACCTT TACAAAAGTA GTTTGTGCTG GTATGATTAC 
ATTGAAGTAA GAGATGGTTA CTGGAGGAAG TCACCTCTCC TTGGTAGATT CTGTGGGGAC 
AAAGTGGCTG GAGTTCTTAC ATCTACGGAC AGCAGAATGT GGATTGAGTT TCGTAGCAGC 
AGTAACTGGG TAGGAAAAGG GTTTGCAGCT GTCTATGAAG CGATTTGTGG AGGGGAGATA 
AGGAAAAACG AAGGGCAGAT TCAGTCTCCC AATTACCCCG ATGACTACCG ACCAATGAAG 
GAGTGTGTAT GGAAAATAAT GGTGTCCGAG GGCTACCATG TTGGACTGAC CTTTCAGGCC 
TTTGAGATCG AAAGACATGA CAGCTGTGCC TATGACCACC TAGAAGTTCG AGATGGAGCC 
AGTGAGAACA GCCCTTTGAT AGGACGGTTC TGTGGTTATG ACAAACCTGA AGATATAAGG 
TCTACTTCCA ACACCCTGTG GATGAAGTTT GTCTCTGACG GGACTGTGAA CAAGGCAGGG 
TTTGCTGCGA ACTTTTTTAA AGAGGAAGAT GAGTGTGCCA AACCTGACCG AGGAGGCTGT 
GAACAGAGGT GTCTTAACAC ACTAGGCAGC TACCAGTGTG CCTGTGAGCC TGGCTATGAA 
CTGGGGCCAG ACAGAAGAAG CTGTGAAGCT GCTTGCGGAG GACTTCTGAC GAAGCTCAAT 
GGCACCATAA CCACCCCCGG CTGGCCCAAA GAGTACCCTC CAAACAAAAA CTGTGTGTGG 
CAAGTGATCG CGCCAAGCCA GTACAGAATC TCTGTGAAGT TTGAGTTTTT TGAATTGGAA 
GGCAATGAAG TTTGCAAATA CGATTACGTG GAGATCTGGA GCGGCCTTTC CTCTGAGTCT 
AAACTGCATG GCAAGTTCTG TGGCGCTGAC ATACCTGAAG TGATGACTTC CCATTTCAAC 
AACATGAGGA TTGAATTCAA GTCAGACAAC ACTGTATCCA AGAAGGGCTT CAAAGCACAT 
TTTTTCTCAG ATAAGGATGA GTGTTCAAAG GATAATGGTG GCTGTCAGCA TGAGTGTGTC 
AACACGATGG GAAGTTACAC GTGTCAGTGC CGGAATGGAT TCGTGTTGCA TGAGAACAAG 
CATGATTGCA AGGAAGCCGA GTGTGAACAG AAGATACACA GCCCAAGTGG TCTCATCACC 
AGTCCCAACT GGCCAGACAA GTATCCAAGC AGGAAAGAGT GCACGTGGGT GATCAGTGCC 
ATTCCTGGCC ACCGCATCAC ATTAGCCTTC AATGAGTTTG AGGTTGAACA ACATCAAGAA 
TGTGCTTATG ATCACTTGGA AATTTTTGAT GGAGAAACGG AGAAGTCACC AATACTTGGC 
CGACTATGTG GCAGCAAGAT ACCAGATCCC CTCATGGCTA CTGGGAATGA AATGTTTATT 
CGGTTTATTT CTGATGCCTC TGTTCAAAGA AAAGGCTTTC AAGCTACACA TTCCACAGAG 
TGTGGTGGTC GATTGAAAGC AGAGTCAAAG CCTAGAGACC TGTACTCCCA TGCTCAGTTT 
GGTGATAATA ACTACCCAGG ACAACTGGAC TGTGAATGGT TGTTGGTGTC AGAACGAGGA 
TCTCGACTTG AATTGTCCTT CCAGACATTC GAAGTAGAAG AAGAAGCTGA CTGTGGCTAT 
GACTATGTTG AAGTCTTTGA TGGTCTCAGT TCAAAAGCTG TGGGTCTTGG TAGATTCTGT 
GGGTCAGGGC CACCAGAAGA AATCTATTCA ATTGGAGATG TGGCTTTGAT TCATTTCCAC 
ACAGATGACA CTATCAACAA GAAAGGATTT TATATAAGAT ATAAAAGTAT AAGATACCCG 
GAAACGATGC ATGCCAAGAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tll1 ( XM_006509314.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509377.1 Mus musculus tolloid-like (Tll1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu40769
Clone ID Related Accession (Same CDS sequence)	XM_006509314.3 , XM_006509314.4
Accession Version	XM_006509314.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tolloid-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509314.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAG GTCAGAGGCA ATCTGTGTTT TGGGGTGACA TCGCCTTAGA TGATGAAGAC 
TTAAATATCT TCCAAATAGA CAGGACAATT GACCTGACCC AGAGCCCCTT TGGAAAACTT 
GGACATATTA CAGGTGGCTT TGGAGACCAT GGCATGCCAA AGAAGCGAGG GGCACTCTAC 
CAACTTATAG AGAGGATCAG AAGAATTGGC TCTGGCTTGG AGCAAAATAA CACGATGAAG 
GGAAAAGCAC CTCCAAAATT GTCAGAGCAA AGTGAGAAAA ATCGAGTTCC CAGAGCTGCT 
ACCTCAAGAA CGGAAAGGAT ATGGCCTGGG GGTGTCATTC CTTATGTCAT AGGAGGAAAC 
TTTACTGGCA GCCAGAGAGC CATGTTCAAG CAGGCCATGA GACACTGGGA AAAGCACACC 
TGTGTGACGT TCACTGAGAG AAGTGATGAA GAAAGTTATA TTGTGTTCAC CTACAGGCCT 
TGTGGATGCT GCTCCTATGT TGGTCGGCGG GGAAATGGCC CTCAGGCCAT CTCTATTGGC 
AAGAACTGTG ACAAGTTTGG AATTGTTGTT CATGAACTGG GCCACGTGAT AGGCTTCTGG 
CATGAACATA CCCGCCCAGA CCGAGACAAC CATGTCACCA TCATTAGAGA GAACATCCAG 
CCAGGTCAAG AGTACAATTT TCTAAAGATG GAGCCTGGAG AAGTGAACTC TCTTGGGGAA 
AGATATGATT TTGACAGTAT CATGCACTAC GCCAGGAACA CCTTCTCAAG AGGGATGTTT 
TTAGACACAA TACTCCCCTC CCGTGATGAT AATGGCATTC GTCCTGCAAT TGGTCAACGG 
ACCCGGTTAA GCAAAGGAGA CATTGCACAA GCAAGAAAGC TGTATCGATG CCCAGCATGT 
GGAGAAACCC TGCAAGAATC CAGTGGCAAC CTTTCTTCCC CAGGATTCCC AAATGGCTAC 
CCTTCCTACA CACACTGCAT CTGGAGAGTG TCTGTGACCC CGGGAGAAAA GATTGTCTTG 
AATTTTACCA CAATGGACCT TTACAAAAGT AGTTTGTGCT GGTATGATTA CATTGAAGTA 
AGAGATGGTT ACTGGAGGAA GTCACCTCTC CTTGGTAGAT TCTGTGGGGA CAAAGTGGCT 
GGAGTTCTTA CATCTACGGA CAGCAGAATG TGGATTGAGT TTCGTAGCAG CAGTAACTGG 
GTAGGAAAAG GGTTTGCAGC TGTCTATGAA GCGATTTGTG GAGGGGAGAT AAGGAAAAAC 
GAAGGGCAGA TTCAGTCTCC CAATTACCCC GATGACTACC GACCAATGAA GGAGTGTGTA 
TGGAAAATAA TGGTGTCCGA GGGCTACCAT GTTGGACTGA CCTTTCAGGC CTTTGAGATC 
GAAAGACATG ACAGCTGTGC CTATGACCAC CTAGAAGTTC GAGATGGAGC CAGTGAGAAC 
AGCCCTTTGA TAGGACGGTT CTGTGGTTAT GACAAACCTG AAGATATAAG GTCTACTTCC 
AACACCCTGT GGATGAAGTT TGTCTCTGAC GGGACTGTGA ACAAGGCAGG GTTTGCTGCG 
AACTTTTTTA AAGAGGAAGA TGAGTGTGCC AAACCTGACC GAGGAGGCTG TGAACAGAGG 
TGTCTTAACA CACTAGGCAG CTACCAGTGT GCCTGTGAGC CTGGCTATGA ACTGGGGCCA 
GACAGAAGAA GCTGTGAAGC TGCTTGCGGA GGACTTCTGA CGAAGCTCAA TGGCACCATA 
ACCACCCCCG GCTGGCCCAA AGAGTACCCT CCAAACAAAA ACTGTGTGTG GCAAGTGATC 
GCGCCAAGCC AGTACAGAAT CTCTGTGAAG TTTGAGTTTT TTGAATTGGA AGGCAATGAA 
GTTTGCAAAT ACGATTACGT GGAGATCTGG AGCGGCCTTT CCTCTGAGTC TAAACTGCAT 
GGCAAGTTCT GTGGCGCTGA CATACCTGAA GTGATGACTT CCCATTTCAA CAACATGAGG 
ATTGAATTCA AGTCAGACAA CACTGTATCC AAGAAGGGCT TCAAAGCACA TTTTTTCTCA 
GATAAGGATG AGTGTTCAAA GGATAATGGT GGCTGTCAGC ATGAGTGTGT CAACACGATG 
GGAAGTTACA CGTGTCAGTG CCGGAATGGA TTCGTGTTGC ATGAGAACAA GCATGATTGC 
AAGGAAGCCG AGTGTGAACA GAAGATACAC AGCCCAAGTG GTCTCATCAC CAGTCCCAAC 
TGGCCAGACA AGTATCCAAG CAGGAAAGAG TGCACGTGGG TGATCAGTGC CATTCCTGGC 
CACCGCATCA CATTAGCCTT CAATGAGTTT GAGGTTGAAC AACATCAAGA ATGTGCTTAT 
GATCACTTGG AAATTTTTGA TGGAGAAACG GAGAAGTCAC CAATACTTGG CCGACTATGT 
GGCAGCAAGA TACCAGATCC CCTCATGGCT ACTGGGAATG AAATGTTTAT TCGGTTTATT 
TCTGATGCCT CTGTTCAAAG AAAAGGCTTT CAAGCTACAC ATTCCACAGA GTGTGGTGGT 
CGATTGAAAG CAGAGTCAAA GCCTAGAGAC CTGTACTCCC ATGCTCAGTT TGGTGATAAT 
AACTACCCAG GACAACTGGA CTGTGAATGG TTGTTGGTGT CAGAACGAGG ATCTCGACTT 
GAATTGTCCT TCCAGACATT CGAAGTAGAA GAAGAAGCTG ACTGTGGCTA TGACTATGTT 
GAAGTCTTTG ATGGTCTCAG TTCAAAAGCT GTGGGTCTTG GTAGATTCTG TGGGTCAGGG 
CCACCAGAAG AAATCTATTC AATTGGAGAT GTGGCTTTGA TTCATTTCCA CACAGATGAC 
ACTATCAACA AGAAAGGATT TTATATAAGA TATAAAAGTA TAAGATACCC GGAAACGATG 
CATGCCAAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509377.1
CDS	429..3323
Translation	MMGGQRQSVFWGDIALDDEDLNIFQIDRTIDLTQSPFGKLGHITGGFGDHGMPKKRGALYQLIERIRRIGSGLEQNNTMKGKAPPKLSEQSEKNRVPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFTERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESSGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKVAGVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKIMVSEGYHVGLTFQAFEIERHDSCAYDHLEVRDGASENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVIAPSQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGADIPEVMTSHFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYTCQCRNGFVLHENKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWVISAIPGHRITLAFNEFEVEQHQECAYDHLEIFDGETEKSPILGRLCGSKIPDPLMATGNEMFIRFISDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQLDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVEVFDGLSSKAVGLGRFCGSGPPEEIYSIGDVALIHFHTDDTINKKGFYIRYKSIRYPETMHAKN

Target ORF information:

RefSeq Version	XM_006509314.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tolloid-like (Tll1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509314.4

ORF Insert Sequence:

ATGATGGGAG GTCAGAGGCA ATCTGTGTTT TGGGGTGACA TCGCCTTAGA TGATGAAGAC 
TTAAATATCT TCCAAATAGA CAGGACAATT GACCTGACCC AGAGCCCCTT TGGAAAACTT 
GGACATATTA CAGGTGGCTT TGGAGACCAT GGCATGCCAA AGAAGCGAGG GGCACTCTAC 
CAACTTATAG AGAGGATCAG AAGAATTGGC TCTGGCTTGG AGCAAAATAA CACGATGAAG 
GGAAAAGCAC CTCCAAAATT GTCAGAGCAA AGTGAGAAAA ATCGAGTTCC CAGAGCTGCT 
ACCTCAAGAA CGGAAAGGAT ATGGCCTGGG GGTGTCATTC CTTATGTCAT AGGAGGAAAC 
TTTACTGGCA GCCAGAGAGC CATGTTCAAG CAGGCCATGA GACACTGGGA AAAGCACACC 
TGTGTGACGT TCACTGAGAG AAGTGATGAA GAAAGTTATA TTGTGTTCAC CTACAGGCCT 
TGTGGATGCT GCTCCTATGT TGGTCGGCGG GGAAATGGCC CTCAGGCCAT CTCTATTGGC 
AAGAACTGTG ACAAGTTTGG AATTGTTGTT CATGAACTGG GCCACGTGAT AGGCTTCTGG 
CATGAACATA CCCGCCCAGA CCGAGACAAC CATGTCACCA TCATTAGAGA GAACATCCAG 
CCAGGTCAAG AGTACAATTT TCTAAAGATG GAGCCTGGAG AAGTGAACTC TCTTGGGGAA 
AGATATGATT TTGACAGTAT CATGCACTAC GCCAGGAACA CCTTCTCAAG AGGGATGTTT 
TTAGACACAA TACTCCCCTC CCGTGATGAT AATGGCATTC GTCCTGCAAT TGGTCAACGG 
ACCCGGTTAA GCAAAGGAGA CATTGCACAA GCAAGAAAGC TGTATCGATG CCCAGCATGT 
GGAGAAACCC TGCAAGAATC CAGTGGCAAC CTTTCTTCCC CAGGATTCCC AAATGGCTAC 
CCTTCCTACA CACACTGCAT CTGGAGAGTG TCTGTGACCC CGGGAGAAAA GATTGTCTTG 
AATTTTACCA CAATGGACCT TTACAAAAGT AGTTTGTGCT GGTATGATTA CATTGAAGTA 
AGAGATGGTT ACTGGAGGAA GTCACCTCTC CTTGGTAGAT TCTGTGGGGA CAAAGTGGCT 
GGAGTTCTTA CATCTACGGA CAGCAGAATG TGGATTGAGT TTCGTAGCAG CAGTAACTGG 
GTAGGAAAAG GGTTTGCAGC TGTCTATGAA GCGATTTGTG GAGGGGAGAT AAGGAAAAAC 
GAAGGGCAGA TTCAGTCTCC CAATTACCCC GATGACTACC GACCAATGAA GGAGTGTGTA 
TGGAAAATAA TGGTGTCCGA GGGCTACCAT GTTGGACTGA CCTTTCAGGC CTTTGAGATC 
GAAAGACATG ACAGCTGTGC CTATGACCAC CTAGAAGTTC GAGATGGAGC CAGTGAGAAC 
AGCCCTTTGA TAGGACGGTT CTGTGGTTAT GACAAACCTG AAGATATAAG GTCTACTTCC 
AACACCCTGT GGATGAAGTT TGTCTCTGAC GGGACTGTGA ACAAGGCAGG GTTTGCTGCG 
AACTTTTTTA AAGAGGAAGA TGAGTGTGCC AAACCTGACC GAGGAGGCTG TGAACAGAGG 
TGTCTTAACA CACTAGGCAG CTACCAGTGT GCCTGTGAGC CTGGCTATGA ACTGGGGCCA 
GACAGAAGAA GCTGTGAAGC TGCTTGCGGA GGACTTCTGA CGAAGCTCAA TGGCACCATA 
ACCACCCCCG GCTGGCCCAA AGAGTACCCT CCAAACAAAA ACTGTGTGTG GCAAGTGATC 
GCGCCAAGCC AGTACAGAAT CTCTGTGAAG TTTGAGTTTT TTGAATTGGA AGGCAATGAA 
GTTTGCAAAT ACGATTACGT GGAGATCTGG AGCGGCCTTT CCTCTGAGTC TAAACTGCAT 
GGCAAGTTCT GTGGCGCTGA CATACCTGAA GTGATGACTT CCCATTTCAA CAACATGAGG 
ATTGAATTCA AGTCAGACAA CACTGTATCC AAGAAGGGCT TCAAAGCACA TTTTTTCTCA 
GATAAGGATG AGTGTTCAAA GGATAATGGT GGCTGTCAGC ATGAGTGTGT CAACACGATG 
GGAAGTTACA CGTGTCAGTG CCGGAATGGA TTCGTGTTGC ATGAGAACAA GCATGATTGC 
AAGGAAGCCG AGTGTGAACA GAAGATACAC AGCCCAAGTG GTCTCATCAC CAGTCCCAAC 
TGGCCAGACA AGTATCCAAG CAGGAAAGAG TGCACGTGGG TGATCAGTGC CATTCCTGGC 
CACCGCATCA CATTAGCCTT CAATGAGTTT GAGGTTGAAC AACATCAAGA ATGTGCTTAT 
GATCACTTGG AAATTTTTGA TGGAGAAACG GAGAAGTCAC CAATACTTGG CCGACTATGT 
GGCAGCAAGA TACCAGATCC CCTCATGGCT ACTGGGAATG AAATGTTTAT TCGGTTTATT 
TCTGATGCCT CTGTTCAAAG AAAAGGCTTT CAAGCTACAC ATTCCACAGA GTGTGGTGGT 
CGATTGAAAG CAGAGTCAAA GCCTAGAGAC CTGTACTCCC ATGCTCAGTT TGGTGATAAT 
AACTACCCAG GACAACTGGA CTGTGAATGG TTGTTGGTGT CAGAACGAGG ATCTCGACTT 
GAATTGTCCT TCCAGACATT CGAAGTAGAA GAAGAAGCTG ACTGTGGCTA TGACTATGTT 
GAAGTCTTTG ATGGTCTCAG TTCAAAAGCT GTGGGTCTTG GTAGATTCTG TGGGTCAGGG 
CCACCAGAAG AAATCTATTC AATTGGAGAT GTGGCTTTGA TTCATTTCCA CACAGATGAC 
ACTATCAACA AGAAAGGATT TTATATAAGA TATAAAAGTA TAAGATACCC GGAAACGATG 
CATGCCAAGA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tll1 ( XM_011242185.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240487.1 Mus musculus tolloid-like (Tll1), transcript variant X2, mRNA.

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CloneID	OMu68921
Clone ID Related Accession (Same CDS sequence)	XM_011242185.3 , XM_011242185.2
Accession Version	XM_011242185.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tolloid-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242185.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTGA CAGTATCATG CACTACGCCA GGAACACCTT CTCAAGGTTG GAGTCTCAGA 
GGGATGTTTT TAGACACAAT ACTCCCCTCC CGTGATGATA ATGGCATTCG TCCTGCAATT 
GGTCAACGGA CCCGGTTAAG CAAAGGAGAC ATTGCACAAG CAAGAAAGCT GTATCGATGC 
CCAGCATGTG GAGAAACCCT GCAAGAATCC AGTGGCAACC TTTCTTCCCC AGGATTCCCA 
AATGGCTACC CTTCCTACAC ACACTGCATC TGGAGAGTGT CTGTGACCCC GGGAGAAAAG 
ATTGTCTTGA ATTTTACCAC AATGGACCTT TACAAAAGTA GTTTGTGCTG GTATGATTAC 
ATTGAAGTAA GAGATGGTTA CTGGAGGAAG TCACCTCTCC TTGGTAGATT CTGTGGGGAC 
AAAGTGGCTG GAGTTCTTAC ATCTACGGAC AGCAGAATGT GGATTGAGTT TCGTAGCAGC 
AGTAACTGGG TAGGAAAAGG GTTTGCAGCT GTCTATGAAG CGATTTGTGG AGGGGAGATA 
AGGAAAAACG AAGGGCAGAT TCAGTCTCCC AATTACCCCG ATGACTACCG ACCAATGAAG 
GAGTGTGTAT GGAAAATAAT GGTGTCCGAG GGCTACCATG TTGGACTGAC CTTTCAGGCC 
TTTGAGATCG AAAGACATGA CAGCTGTGCC TATGACCACC TAGAAGTTCG AGATGGAGCC 
AGTGAGAACA GCCCTTTGAT AGGACGGTTC TGTGGTTATG ACAAACCTGA AGATATAAGG 
TCTACTTCCA ACACCCTGTG GATGAAGTTT GTCTCTGACG GGACTGTGAA CAAGGCAGGG 
TTTGCTGCGA ACTTTTTTAA AGAGGAAGAT GAGTGTGCCA AACCTGACCG AGGAGGCTGT 
GAACAGAGGT GTCTTAACAC ACTAGGCAGC TACCAGTGTG CCTGTGAGCC TGGCTATGAA 
CTGGGGCCAG ACAGAAGAAG CTGTGAAGCT GCTTGCGGAG GACTTCTGAC GAAGCTCAAT 
GGCACCATAA CCACCCCCGG CTGGCCCAAA GAGTACCCTC CAAACAAAAA CTGTGTGTGG 
CAAGTGATCG CGCCAAGCCA GTACAGAATC TCTGTGAAGT TTGAGTTTTT TGAATTGGAA 
GGCAATGAAG TTTGCAAATA CGATTACGTG GAGATCTGGA GCGGCCTTTC CTCTGAGTCT 
AAACTGCATG GCAAGTTCTG TGGCGCTGAC ATACCTGAAG TGATGACTTC CCATTTCAAC 
AACATGAGGA TTGAATTCAA GTCAGACAAC ACTGTATCCA AGAAGGGCTT CAAAGCACAT 
TTTTTCTCAG ATAAGGATGA GTGTTCAAAG GATAATGGTG GCTGTCAGCA TGAGTGTGTC 
AACACGATGG GAAGTTACAC GTGTCAGTGC CGGAATGGAT TCGTGTTGCA TGAGAACAAG 
CATGATTGCA AGGAAGCCGA GTGTGAACAG AAGATACACA GCCCAAGTGG TCTCATCACC 
AGTCCCAACT GGCCAGACAA GTATCCAAGC AGGAAAGAGT GCACGTGGGT GATCAGTGCC 
ATTCCTGGCC ACCGCATCAC ATTAGCCTTC AATGAGTTTG AGGTTGAACA ACATCAAGAA 
TGTGCTTATG ATCACTTGGA AATTTTTGAT GGAGAAACGG AGAAGTCACC AATACTTGGC 
CGACTATGTG GCAGCAAGAT ACCAGATCCC CTCATGGCTA CTGGGAATGA AATGTTTATT 
CGGTTTATTT CTGATGCCTC TGTTCAAAGA AAAGGCTTTC AAGCTACACA TTCCACAGAG 
TGTGGTGGTC GATTGAAAGC AGAGTCAAAG CCTAGAGACC TGTACTCCCA TGCTCAGTTT 
GGTGATAATA ACTACCCAGG ACAACTGGAC TGTGAATGGT TGTTGGTGTC AGAACGAGGA 
TCTCGACTTG AATTGTCCTT CCAGACATTC GAAGTAGAAG AAGAAGCTGA CTGTGGCTAT 
GACTATGTTG AAGTCTTTGA TGGTCTCAGT TCAAAAGCTG TGGGTCTTGG TAGATTCTGT 
GGGTCAGGGC CACCAGAAGA AATCTATTCA ATTGGAGATG TGGCTTTGAT TCATTTCCAC 
ACAGATGACA CTATCAACAA GAAAGGATTT TATATAAGAT ATAAAAGTAT AAGATACCCG 
GAAACGATGC ATGCCAAGAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240487.1
CDS	1122..3305
Translation	MILTVSCTTPGTPSQGWSLRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESSGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKVAGVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKIMVSEGYHVGLTFQAFEIERHDSCAYDHLEVRDGASENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVIAPSQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGADIPEVMTSHFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYTCQCRNGFVLHENKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWVISAIPGHRITLAFNEFEVEQHQECAYDHLEIFDGETEKSPILGRLCGSKIPDPLMATGNEMFIRFISDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQLDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVEVFDGLSSKAVGLGRFCGSGPPEEIYSIGDVALIHFHTDDTINKKGFYIRYKSIRYPETMHAKN

Target ORF information:

RefSeq Version	XM_011242185.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tolloid-like (Tll1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011242185.3

ORF Insert Sequence:

ATGATTTTGA CAGTATCATG CACTACGCCA GGAACACCTT CTCAAGGTTG GAGTCTCAGA 
GGGATGTTTT TAGACACAAT ACTCCCCTCC CGTGATGATA ATGGCATTCG TCCTGCAATT 
GGTCAACGGA CCCGGTTAAG CAAAGGAGAC ATTGCACAAG CAAGAAAGCT GTATCGATGC 
CCAGCATGTG GAGAAACCCT GCAAGAATCC AGTGGCAACC TTTCTTCCCC AGGATTCCCA 
AATGGCTACC CTTCCTACAC ACACTGCATC TGGAGAGTGT CTGTGACCCC GGGAGAAAAG 
ATTGTCTTGA ATTTTACCAC AATGGACCTT TACAAAAGTA GTTTGTGCTG GTATGATTAC 
ATTGAAGTAA GAGATGGTTA CTGGAGGAAG TCACCTCTCC TTGGTAGATT CTGTGGGGAC 
AAAGTGGCTG GAGTTCTTAC ATCTACGGAC AGCAGAATGT GGATTGAGTT TCGTAGCAGC 
AGTAACTGGG TAGGAAAAGG GTTTGCAGCT GTCTATGAAG CGATTTGTGG AGGGGAGATA 
AGGAAAAACG AAGGGCAGAT TCAGTCTCCC AATTACCCCG ATGACTACCG ACCAATGAAG 
GAGTGTGTAT GGAAAATAAT GGTGTCCGAG GGCTACCATG TTGGACTGAC CTTTCAGGCC 
TTTGAGATCG AAAGACATGA CAGCTGTGCC TATGACCACC TAGAAGTTCG AGATGGAGCC 
AGTGAGAACA GCCCTTTGAT AGGACGGTTC TGTGGTTATG ACAAACCTGA AGATATAAGG 
TCTACTTCCA ACACCCTGTG GATGAAGTTT GTCTCTGACG GGACTGTGAA CAAGGCAGGG 
TTTGCTGCGA ACTTTTTTAA AGAGGAAGAT GAGTGTGCCA AACCTGACCG AGGAGGCTGT 
GAACAGAGGT GTCTTAACAC ACTAGGCAGC TACCAGTGTG CCTGTGAGCC TGGCTATGAA 
CTGGGGCCAG ACAGAAGAAG CTGTGAAGCT GCTTGCGGAG GACTTCTGAC GAAGCTCAAT 
GGCACCATAA CCACCCCCGG CTGGCCCAAA GAGTACCCTC CAAACAAAAA CTGTGTGTGG 
CAAGTGATCG CGCCAAGCCA GTACAGAATC TCTGTGAAGT TTGAGTTTTT TGAATTGGAA 
GGCAATGAAG TTTGCAAATA CGATTACGTG GAGATCTGGA GCGGCCTTTC CTCTGAGTCT 
AAACTGCATG GCAAGTTCTG TGGCGCTGAC ATACCTGAAG TGATGACTTC CCATTTCAAC 
AACATGAGGA TTGAATTCAA GTCAGACAAC ACTGTATCCA AGAAGGGCTT CAAAGCACAT 
TTTTTCTCAG ATAAGGATGA GTGTTCAAAG GATAATGGTG GCTGTCAGCA TGAGTGTGTC 
AACACGATGG GAAGTTACAC GTGTCAGTGC CGGAATGGAT TCGTGTTGCA TGAGAACAAG 
CATGATTGCA AGGAAGCCGA GTGTGAACAG AAGATACACA GCCCAAGTGG TCTCATCACC 
AGTCCCAACT GGCCAGACAA GTATCCAAGC AGGAAAGAGT GCACGTGGGT GATCAGTGCC 
ATTCCTGGCC ACCGCATCAC ATTAGCCTTC AATGAGTTTG AGGTTGAACA ACATCAAGAA 
TGTGCTTATG ATCACTTGGA AATTTTTGAT GGAGAAACGG AGAAGTCACC AATACTTGGC 
CGACTATGTG GCAGCAAGAT ACCAGATCCC CTCATGGCTA CTGGGAATGA AATGTTTATT 
CGGTTTATTT CTGATGCCTC TGTTCAAAGA AAAGGCTTTC AAGCTACACA TTCCACAGAG 
TGTGGTGGTC GATTGAAAGC AGAGTCAAAG CCTAGAGACC TGTACTCCCA TGCTCAGTTT 
GGTGATAATA ACTACCCAGG ACAACTGGAC TGTGAATGGT TGTTGGTGTC AGAACGAGGA 
TCTCGACTTG AATTGTCCTT CCAGACATTC GAAGTAGAAG AAGAAGCTGA CTGTGGCTAT 
GACTATGTTG AAGTCTTTGA TGGTCTCAGT TCAAAAGCTG TGGGTCTTGG TAGATTCTGT 
GGGTCAGGGC CACCAGAAGA AATCTATTCA ATTGGAGATG TGGCTTTGAT TCATTTCCAC 
ACAGATGACA CTATCAACAA GAAAGGATTT TATATAAGAT ATAAAAGTAT AAGATACCCG 
GAAACGATGC ATGCCAAGAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tll1 ( NM_009390.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033416.2 Mus musculus tolloid-like (Tll1), mRNA.

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CloneID	OMu04058
Clone ID Related Accession (Same CDS sequence)	NM_009390.2 , NM_009390.3
Accession Version	NM_009390.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-27
Organism	Mus musculus(House mouse)
Product	tolloid-like protein 1 precursor
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U34042.1, AK139103.1 and BX524877.1. On or before Jun 14, 2009 this sequence version replaced XM_001475474.1, XM_001475852.1, NM_009390.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U34042.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGGTTTGC AAGCGCTCTC CCCGAGGATG CTCCTGTGGT TGGTGGTCTC GGGTATTGTT 
TTCTCCCGGG TGCTGTGGGT CTGCGCTGGC CTCGATTATG ATTACACTTT TGATGGGAAC 
GAAGAGGACA AAACGGAGCC TATAGATTAC AAGGACCCGT GCAAAGCTGC TGTGTTTTGG 
GGTGACATCG CCTTAGATGA TGAAGACTTA AATATCTTCC AAATAGACAG GACAATTGAC 
CTGACCCAGA GCCCCTTTGG AAAACTTGGA CATATTACAG GTGGCTTTGG AGACCATGGC 
ATGCCAAAGA AGCGAGGGGC ACTCTACCAA CTTATAGAGA GGATCAGAAG AATTGGCTCT 
GGCTTGGAGC AAAATAACAC GATGAAGGGA AAAGCACCTC CAAAATTGTC AGAGCAAAGT 
GAGAAAAATC GAGTTCCCAG AGCTGCTACC TCAAGAACGG AAAGGATATG GCCTGGGGGT 
GTCATTCCTT ATGTCATAGG AGGAAACTTT ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGAC ACTGGGAAAA GCACACCTGT GTGACGTTCA CTGAGAGAAG TGATGAAGAA 
AGTTATATTG TGTTCACCTA CAGGCCTTGT GGATGCTGCT CCTATGTTGG TCGGCGGGGA 
AATGGCCCTC AGGCCATCTC TATTGGCAAG AACTGTGACA AGTTTGGAAT TGTTGTTCAT 
GAACTGGGCC ACGTGATAGG CTTCTGGCAT GAACATACCC GCCCAGACCG AGACAACCAT 
GTCACCATCA TTAGAGAGAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT AAAGATGGAG 
CCTGGAGAAG TGAACTCTCT TGGGGAAAGA TATGATTTTG ACAGTATCAT GCACTACGCC 
AGGAACACCT TCTCAAGAGG GATGTTTTTA GACACAATAC TCCCCTCCCG TGATGATAAT 
GGCATTCGTC CTGCAATTGG TCAACGGACC CGGTTAAGCA AAGGAGACAT TGCACAAGCA 
AGAAAGCTGT ATCGATGCCC AGCATGTGGA GAAACCCTGC AAGAATCCAG TGGCAACCTT 
TCTTCCCCAG GATTCCCAAA TGGCTACCCT TCCTACACAC ACTGCATCTG GAGAGTGTCT 
GTGACCCCGG GAGAAAAGAT TGTCTTGAAT TTTACCACAA TGGACCTTTA CAAAAGTAGT 
TTGTGCTGGT ATGATTACAT TGAAGTAAGA GATGGTTACT GGAGGAAGTC ACCTCTCCTT 
GGTAGATTCT GTGGGGACAA AGTGGCTGGA GTTCTTACAT CTACGGACAG CAGAATGTGG 
ATTGAGTTTC GTAGCAGCAG TAACTGGGTA GGAAAAGGGT TTGCAGCTGT CTATGAAGCG 
ATTTGTGGAG GGGAGATAAG GAAAAACGAA GGGCAGATTC AGTCTCCCAA TTACCCCGAT 
GACTACCGAC CAATGAAGGA GTGTGTATGG AAAATAATGG TGTCCGAGGG CTACCATGTT 
GGACTGACCT TTCAGGCCTT TGAGATCGAA AGACATGACA GCTGTGCCTA TGACCACCTA 
GAAGTTCGAG ATGGAGCCAG TGAGAACAGC CCTTTGATAG GACGGTTCTG TGGTTATGAC 
AAACCTGAAG ATATAAGGTC TACTTCCAAC ACCCTGTGGA TGAAGTTTGT CTCTGACGGG 
ACTGTGAACA AGGCAGGGTT TGCTGCGAAC TTTTTTAAAG AGGAAGATGA GTGTGCCAAA 
CCTGACCGAG GAGGCTGTGA ACAGAGGTGT CTTAACACAC TAGGCAGCTA CCAGTGTGCC 
TGTGAGCCTG GCTATGAACT GGGGCCAGAC AGAAGAAGCT GTGAAGCTGC TTGCGGAGGA 
CTTCTGACGA AGCTCAATGG CACCATAACC ACCCCCGGCT GGCCCAAAGA GTACCCTCCA 
AACAAAAACT GTGTGTGGCA AGTGATCGCG CCAAGCCAGT ACAGAATCTC TGTGAAGTTT 
GAGTTTTTTG AATTGGAAGG CAATGAAGTT TGCAAATACG ATTACGTGGA GATCTGGAGC 
GGCCTTTCCT CTGAGTCTAA ACTGCATGGC AAGTTCTGTG GCGCTGACAT ACCTGAAGTG 
ATGACTTCCC ATTTCAACAA CATGAGGATT GAATTCAAGT CAGACAACAC TGTATCCAAG 
AAGGGCTTCA AAGCACATTT TTTCTCAGAT AAGGATGAGT GTTCAAAGGA TAATGGTGGC 
TGTCAGCATG AGTGTGTCAA CACGATGGGA AGTTACACGT GTCAGTGCCG GAATGGATTC 
GTGTTGCATG AGAACAAGCA TGATTGCAAG GAAGCCGAGT GTGAACAGAA GATACACAGC 
CCAAGTGGTC TCATCACCAG TCCCAACTGG CCAGACAAGT ATCCAAGCAG GAAAGAGTGC 
ACGTGGGTGA TCAGTGCCAT TCCTGGCCAC CGCATCACAT TAGCCTTCAA TGAGTTTGAG 
GTTGAACAAC ATCAAGAATG TGCTTATGAT CACTTGGAAA TTTTTGATGG AGAAACGGAG 
AAGTCACCAA TACTTGGCCG ACTATGTGGC AGCAAGATAC CAGATCCCCT CATGGCTACT 
GGGAATGAAA TGTTTATTCG GTTTATTTCT GATGCCTCTG TTCAAAGAAA AGGCTTTCAA 
GCTACACATT CCACAGAGTG TGGTGGTCGA TTGAAAGCAG AGTCAAAGCC TAGAGACCTG 
TACTCCCATG CTCAGTTTGG TGATAATAAC TACCCAGGAC AACTGGACTG TGAATGGTTG 
TTGGTGTCAG AACGAGGATC TCGACTTGAA TTGTCCTTCC AGACATTCGA AGTAGAAGAA 
GAAGCTGACT GTGGCTATGA CTATGTTGAA GTCTTTGATG GTCTCAGTTC AAAAGCTGTG 
GGTCTTGGTA GATTCTGTGG GTCAGGGCCA CCAGAAGAAA TCTATTCAAT TGGAGATGTG 
GCTTTGATTC ATTTCCACAC AGATGACACT ATCAACAAGA AAGGATTTTA TATAAGATAT 
AAAAGTATAA GATACCCGGA AACGATGCAT GCCAAGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033416.2
CDS	611..3652
Translation	MGLQALSPRMLLWLVVSGIVFSRVLWVCAGLDYDYTFDGNEEDKTEPIDYKDPCKAAVFWGDIALDDEDLNIFQIDRTIDLTQSPFGKLGHITGGFGDHGMPKKRGALYQLIERIRRIGSGLEQNNTMKGKAPPKLSEQSEKNRVPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFTERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESSGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKVAGVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKIMVSEGYHVGLTFQAFEIERHDSCAYDHLEVRDGASENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVIAPSQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGADIPEVMTSHFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYTCQCRNGFVLHENKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWVISAIPGHRITLAFNEFEVEQHQECAYDHLEIFDGETEKSPILGRLCGSKIPDPLMATGNEMFIRFISDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQLDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVEVFDGLSSKAVGLGRFCGSGPPEEIYSIGDVALIHFHTDDTINKKGFYIRYKSIRYPETMHAKN

Target ORF information:

RefSeq Version	NM_009390.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus tolloid-like (Tll1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009390.2

ORF Insert Sequence:

ATGGGTTTGC AAGCGCTCTC CCCGAGGATG CTCCTGTGGT TGGTGGTCTC GGGTATTGTT 
TTCTCCCGGG TGCTGTGGGT CTGCGCTGGC CTCGATTATG ATTACACTTT TGATGGGAAC 
GAAGAGGACA AAACGGAGCC TATAGATTAC AAGGACCCGT GCAAAGCTGC TGTGTTTTGG 
GGTGACATCG CCTTAGATGA TGAAGACTTA AATATCTTCC AAATAGACAG GACAATTGAC 
CTGACCCAGA GCCCCTTTGG AAAACTTGGA CATATTACAG GTGGCTTTGG AGACCATGGC 
ATGCCAAAGA AGCGAGGGGC ACTCTACCAA CTTATAGAGA GGATCAGAAG AATTGGCTCT 
GGCTTGGAGC AAAATAACAC GATGAAGGGA AAAGCACCTC CAAAATTGTC AGAGCAAAGT 
GAGAAAAATC GAGTTCCCAG AGCTGCTACC TCAAGAACGG AAAGGATATG GCCTGGGGGT 
GTCATTCCTT ATGTCATAGG AGGAAACTTT ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGAC ACTGGGAAAA GCACACCTGT GTGACGTTCA CTGAGAGAAG TGATGAAGAA 
AGTTATATTG TGTTCACCTA CAGGCCTTGT GGATGCTGCT CCTATGTTGG TCGGCGGGGA 
AATGGCCCTC AGGCCATCTC TATTGGCAAG AACTGTGACA AGTTTGGAAT TGTTGTTCAT 
GAACTGGGCC ACGTGATAGG CTTCTGGCAT GAACATACCC GCCCAGACCG AGACAACCAT 
GTCACCATCA TTAGAGAGAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT AAAGATGGAG 
CCTGGAGAAG TGAACTCTCT TGGGGAAAGA TATGATTTTG ACAGTATCAT GCACTACGCC 
AGGAACACCT TCTCAAGAGG GATGTTTTTA GACACAATAC TCCCCTCCCG TGATGATAAT 
GGCATTCGTC CTGCAATTGG TCAACGGACC CGGTTAAGCA AAGGAGACAT TGCACAAGCA 
AGAAAGCTGT ATCGATGCCC AGCATGTGGA GAAACCCTGC AAGAATCCAG TGGCAACCTT 
TCTTCCCCAG GATTCCCAAA TGGCTACCCT TCCTACACAC ACTGCATCTG GAGAGTGTCT 
GTGACCCCGG GAGAAAAGAT TGTCTTGAAT TTTACCACAA TGGACCTTTA CAAAAGTAGT 
TTGTGCTGGT ATGATTACAT TGAAGTAAGA GATGGTTACT GGAGGAAGTC ACCTCTCCTT 
GGTAGATTCT GTGGGGACAA AGTGGCTGGA GTTCTTACAT CTACGGACAG CAGAATGTGG 
ATTGAGTTTC GTAGCAGCAG TAACTGGGTA GGAAAAGGGT TTGCAGCTGT CTATGAAGCG 
ATTTGTGGAG GGGAGATAAG GAAAAACGAA GGGCAGATTC AGTCTCCCAA TTACCCCGAT 
GACTACCGAC CAATGAAGGA GTGTGTATGG AAAATAATGG TGTCCGAGGG CTACCATGTT 
GGACTGACCT TTCAGGCCTT TGAGATCGAA AGACATGACA GCTGTGCCTA TGACCACCTA 
GAAGTTCGAG ATGGAGCCAG TGAGAACAGC CCTTTGATAG GACGGTTCTG TGGTTATGAC 
AAACCTGAAG ATATAAGGTC TACTTCCAAC ACCCTGTGGA TGAAGTTTGT CTCTGACGGG 
ACTGTGAACA AGGCAGGGTT TGCTGCGAAC TTTTTTAAAG AGGAAGATGA GTGTGCCAAA 
CCTGACCGAG GAGGCTGTGA ACAGAGGTGT CTTAACACAC TAGGCAGCTA CCAGTGTGCC 
TGTGAGCCTG GCTATGAACT GGGGCCAGAC AGAAGAAGCT GTGAAGCTGC TTGCGGAGGA 
CTTCTGACGA AGCTCAATGG CACCATAACC ACCCCCGGCT GGCCCAAAGA GTACCCTCCA 
AACAAAAACT GTGTGTGGCA AGTGATCGCG CCAAGCCAGT ACAGAATCTC TGTGAAGTTT 
GAGTTTTTTG AATTGGAAGG CAATGAAGTT TGCAAATACG ATTACGTGGA GATCTGGAGC 
GGCCTTTCCT CTGAGTCTAA ACTGCATGGC AAGTTCTGTG GCGCTGACAT ACCTGAAGTG 
ATGACTTCCC ATTTCAACAA CATGAGGATT GAATTCAAGT CAGACAACAC TGTATCCAAG 
AAGGGCTTCA AAGCACATTT TTTCTCAGAT AAGGATGAGT GTTCAAAGGA TAATGGTGGC 
TGTCAGCATG AGTGTGTCAA CACGATGGGA AGTTACACGT GTCAGTGCCG GAATGGATTC 
GTGTTGCATG AGAACAAGCA TGATTGCAAG GAAGCCGAGT GTGAACAGAA GATACACAGC 
CCAAGTGGTC TCATCACCAG TCCCAACTGG CCAGACAAGT ATCCAAGCAG GAAAGAGTGC 
ACGTGGGTGA TCAGTGCCAT TCCTGGCCAC CGCATCACAT TAGCCTTCAA TGAGTTTGAG 
GTTGAACAAC ATCAAGAATG TGCTTATGAT CACTTGGAAA TTTTTGATGG AGAAACGGAG 
AAGTCACCAA TACTTGGCCG ACTATGTGGC AGCAAGATAC CAGATCCCCT CATGGCTACT 
GGGAATGAAA TGTTTATTCG GTTTATTTCT GATGCCTCTG TTCAAAGAAA AGGCTTTCAA 
GCTACACATT CCACAGAGTG TGGTGGTCGA TTGAAAGCAG AGTCAAAGCC TAGAGACCTG 
TACTCCCATG CTCAGTTTGG TGATAATAAC TACCCAGGAC AACTGGACTG TGAATGGTTG 
TTGGTGTCAG AACGAGGATC TCGACTTGAA TTGTCCTTCC AGACATTCGA AGTAGAAGAA 
GAAGCTGACT GTGGCTATGA CTATGTTGAA GTCTTTGATG GTCTCAGTTC AAAAGCTGTG 
GGTCTTGGTA GATTCTGTGG GTCAGGGCCA CCAGAAGAAA TCTATTCAAT TGGAGATGTG 
GCTTTGATTC ATTTCCACAC AGATGACACT ATCAACAAGA AAGGATTTTA TATAAGATAT 
AAAAGTATAA GATACCCGGA AACGATGCAT GCCAAGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tll1 ( NM_009390.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033416.2 Mus musculus tolloid-like (Tll1), mRNA.

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CloneID	OMu04058
Clone ID Related Accession (Same CDS sequence)	NM_009390.2 , NM_009390.3
Accession Version	NM_009390.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	tolloid-like protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK080919.1, AC110622.6, CF734525.1, AK139103.1 and AC149051.4. On Oct 21, 2017 this sequence version replaced NM_009390.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U34042.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGGTTTGC AAGCGCTCTC CCCGAGGATG CTCCTGTGGT TGGTGGTCTC GGGTATTGTT 
TTCTCCCGGG TGCTGTGGGT CTGCGCTGGC CTCGATTATG ATTACACTTT TGATGGGAAC 
GAAGAGGACA AAACGGAGCC TATAGATTAC AAGGACCCGT GCAAAGCTGC TGTGTTTTGG 
GGTGACATCG CCTTAGATGA TGAAGACTTA AATATCTTCC AAATAGACAG GACAATTGAC 
CTGACCCAGA GCCCCTTTGG AAAACTTGGA CATATTACAG GTGGCTTTGG AGACCATGGC 
ATGCCAAAGA AGCGAGGGGC ACTCTACCAA CTTATAGAGA GGATCAGAAG AATTGGCTCT 
GGCTTGGAGC AAAATAACAC GATGAAGGGA AAAGCACCTC CAAAATTGTC AGAGCAAAGT 
GAGAAAAATC GAGTTCCCAG AGCTGCTACC TCAAGAACGG AAAGGATATG GCCTGGGGGT 
GTCATTCCTT ATGTCATAGG AGGAAACTTT ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGAC ACTGGGAAAA GCACACCTGT GTGACGTTCA CTGAGAGAAG TGATGAAGAA 
AGTTATATTG TGTTCACCTA CAGGCCTTGT GGATGCTGCT CCTATGTTGG TCGGCGGGGA 
AATGGCCCTC AGGCCATCTC TATTGGCAAG AACTGTGACA AGTTTGGAAT TGTTGTTCAT 
GAACTGGGCC ACGTGATAGG CTTCTGGCAT GAACATACCC GCCCAGACCG AGACAACCAT 
GTCACCATCA TTAGAGAGAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT AAAGATGGAG 
CCTGGAGAAG TGAACTCTCT TGGGGAAAGA TATGATTTTG ACAGTATCAT GCACTACGCC 
AGGAACACCT TCTCAAGAGG GATGTTTTTA GACACAATAC TCCCCTCCCG TGATGATAAT 
GGCATTCGTC CTGCAATTGG TCAACGGACC CGGTTAAGCA AAGGAGACAT TGCACAAGCA 
AGAAAGCTGT ATCGATGCCC AGCATGTGGA GAAACCCTGC AAGAATCCAG TGGCAACCTT 
TCTTCCCCAG GATTCCCAAA TGGCTACCCT TCCTACACAC ACTGCATCTG GAGAGTGTCT 
GTGACCCCGG GAGAAAAGAT TGTCTTGAAT TTTACCACAA TGGACCTTTA CAAAAGTAGT 
TTGTGCTGGT ATGATTACAT TGAAGTAAGA GATGGTTACT GGAGGAAGTC ACCTCTCCTT 
GGTAGATTCT GTGGGGACAA AGTGGCTGGA GTTCTTACAT CTACGGACAG CAGAATGTGG 
ATTGAGTTTC GTAGCAGCAG TAACTGGGTA GGAAAAGGGT TTGCAGCTGT CTATGAAGCG 
ATTTGTGGAG GGGAGATAAG GAAAAACGAA GGGCAGATTC AGTCTCCCAA TTACCCCGAT 
GACTACCGAC CAATGAAGGA GTGTGTATGG AAAATAATGG TGTCCGAGGG CTACCATGTT 
GGACTGACCT TTCAGGCCTT TGAGATCGAA AGACATGACA GCTGTGCCTA TGACCACCTA 
GAAGTTCGAG ATGGAGCCAG TGAGAACAGC CCTTTGATAG GACGGTTCTG TGGTTATGAC 
AAACCTGAAG ATATAAGGTC TACTTCCAAC ACCCTGTGGA TGAAGTTTGT CTCTGACGGG 
ACTGTGAACA AGGCAGGGTT TGCTGCGAAC TTTTTTAAAG AGGAAGATGA GTGTGCCAAA 
CCTGACCGAG GAGGCTGTGA ACAGAGGTGT CTTAACACAC TAGGCAGCTA CCAGTGTGCC 
TGTGAGCCTG GCTATGAACT GGGGCCAGAC AGAAGAAGCT GTGAAGCTGC TTGCGGAGGA 
CTTCTGACGA AGCTCAATGG CACCATAACC ACCCCCGGCT GGCCCAAAGA GTACCCTCCA 
AACAAAAACT GTGTGTGGCA AGTGATCGCG CCAAGCCAGT ACAGAATCTC TGTGAAGTTT 
GAGTTTTTTG AATTGGAAGG CAATGAAGTT TGCAAATACG ATTACGTGGA GATCTGGAGC 
GGCCTTTCCT CTGAGTCTAA ACTGCATGGC AAGTTCTGTG GCGCTGACAT ACCTGAAGTG 
ATGACTTCCC ATTTCAACAA CATGAGGATT GAATTCAAGT CAGACAACAC TGTATCCAAG 
AAGGGCTTCA AAGCACATTT TTTCTCAGAT AAGGATGAGT GTTCAAAGGA TAATGGTGGC 
TGTCAGCATG AGTGTGTCAA CACGATGGGA AGTTACACGT GTCAGTGCCG GAATGGATTC 
GTGTTGCATG AGAACAAGCA TGATTGCAAG GAAGCCGAGT GTGAACAGAA GATACACAGC 
CCAAGTGGTC TCATCACCAG TCCCAACTGG CCAGACAAGT ATCCAAGCAG GAAAGAGTGC 
ACGTGGGTGA TCAGTGCCAT TCCTGGCCAC CGCATCACAT TAGCCTTCAA TGAGTTTGAG 
GTTGAACAAC ATCAAGAATG TGCTTATGAT CACTTGGAAA TTTTTGATGG AGAAACGGAG 
AAGTCACCAA TACTTGGCCG ACTATGTGGC AGCAAGATAC CAGATCCCCT CATGGCTACT 
GGGAATGAAA TGTTTATTCG GTTTATTTCT GATGCCTCTG TTCAAAGAAA AGGCTTTCAA 
GCTACACATT CCACAGAGTG TGGTGGTCGA TTGAAAGCAG AGTCAAAGCC TAGAGACCTG 
TACTCCCATG CTCAGTTTGG TGATAATAAC TACCCAGGAC AACTGGACTG TGAATGGTTG 
TTGGTGTCAG AACGAGGATC TCGACTTGAA TTGTCCTTCC AGACATTCGA AGTAGAAGAA 
GAAGCTGACT GTGGCTATGA CTATGTTGAA GTCTTTGATG GTCTCAGTTC AAAAGCTGTG 
GGTCTTGGTA GATTCTGTGG GTCAGGGCCA CCAGAAGAAA TCTATTCAAT TGGAGATGTG 
GCTTTGATTC ATTTCCACAC AGATGACACT ATCAACAAGA AAGGATTTTA TATAAGATAT 
AAAAGTATAA GATACCCGGA AACGATGCAT GCCAAGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033416.2
CDS	626..3667
Translation	MGLQALSPRMLLWLVVSGIVFSRVLWVCAGLDYDYTFDGNEEDKTEPIDYKDPCKAAVFWGDIALDDEDLNIFQIDRTIDLTQSPFGKLGHITGGFGDHGMPKKRGALYQLIERIRRIGSGLEQNNTMKGKAPPKLSEQSEKNRVPRAATSRTERIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKHTCVTFTERSDEESYIVFTYRPCGCCSYVGRRGNGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFSRGMFLDTILPSRDDNGIRPAIGQRTRLSKGDIAQARKLYRCPACGETLQESSGNLSSPGFPNGYPSYTHCIWRVSVTPGEKIVLNFTTMDLYKSSLCWYDYIEVRDGYWRKSPLLGRFCGDKVAGVLTSTDSRMWIEFRSSSNWVGKGFAAVYEAICGGEIRKNEGQIQSPNYPDDYRPMKECVWKIMVSEGYHVGLTFQAFEIERHDSCAYDHLEVRDGASENSPLIGRFCGYDKPEDIRSTSNTLWMKFVSDGTVNKAGFAANFFKEEDECAKPDRGGCEQRCLNTLGSYQCACEPGYELGPDRRSCEAACGGLLTKLNGTITTPGWPKEYPPNKNCVWQVIAPSQYRISVKFEFFELEGNEVCKYDYVEIWSGLSSESKLHGKFCGADIPEVMTSHFNNMRIEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQHECVNTMGSYTCQCRNGFVLHENKHDCKEAECEQKIHSPSGLITSPNWPDKYPSRKECTWVISAIPGHRITLAFNEFEVEQHQECAYDHLEIFDGETEKSPILGRLCGSKIPDPLMATGNEMFIRFISDASVQRKGFQATHSTECGGRLKAESKPRDLYSHAQFGDNNYPGQLDCEWLLVSERGSRLELSFQTFEVEEEADCGYDYVEVFDGLSSKAVGLGRFCGSGPPEEIYSIGDVALIHFHTDDTINKKGFYIRYKSIRYPETMHAKN

Target ORF information:

RefSeq Version	NM_009390.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tolloid-like (Tll1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009390.3

ORF Insert Sequence:

ATGGGTTTGC AAGCGCTCTC CCCGAGGATG CTCCTGTGGT TGGTGGTCTC GGGTATTGTT 
TTCTCCCGGG TGCTGTGGGT CTGCGCTGGC CTCGATTATG ATTACACTTT TGATGGGAAC 
GAAGAGGACA AAACGGAGCC TATAGATTAC AAGGACCCGT GCAAAGCTGC TGTGTTTTGG 
GGTGACATCG CCTTAGATGA TGAAGACTTA AATATCTTCC AAATAGACAG GACAATTGAC 
CTGACCCAGA GCCCCTTTGG AAAACTTGGA CATATTACAG GTGGCTTTGG AGACCATGGC 
ATGCCAAAGA AGCGAGGGGC ACTCTACCAA CTTATAGAGA GGATCAGAAG AATTGGCTCT 
GGCTTGGAGC AAAATAACAC GATGAAGGGA AAAGCACCTC CAAAATTGTC AGAGCAAAGT 
GAGAAAAATC GAGTTCCCAG AGCTGCTACC TCAAGAACGG AAAGGATATG GCCTGGGGGT 
GTCATTCCTT ATGTCATAGG AGGAAACTTT ACTGGCAGCC AGAGAGCCAT GTTCAAGCAG 
GCCATGAGAC ACTGGGAAAA GCACACCTGT GTGACGTTCA CTGAGAGAAG TGATGAAGAA 
AGTTATATTG TGTTCACCTA CAGGCCTTGT GGATGCTGCT CCTATGTTGG TCGGCGGGGA 
AATGGCCCTC AGGCCATCTC TATTGGCAAG AACTGTGACA AGTTTGGAAT TGTTGTTCAT 
GAACTGGGCC ACGTGATAGG CTTCTGGCAT GAACATACCC GCCCAGACCG AGACAACCAT 
GTCACCATCA TTAGAGAGAA CATCCAGCCA GGTCAAGAGT ACAATTTTCT AAAGATGGAG 
CCTGGAGAAG TGAACTCTCT TGGGGAAAGA TATGATTTTG ACAGTATCAT GCACTACGCC 
AGGAACACCT TCTCAAGAGG GATGTTTTTA GACACAATAC TCCCCTCCCG TGATGATAAT 
GGCATTCGTC CTGCAATTGG TCAACGGACC CGGTTAAGCA AAGGAGACAT TGCACAAGCA 
AGAAAGCTGT ATCGATGCCC AGCATGTGGA GAAACCCTGC AAGAATCCAG TGGCAACCTT 
TCTTCCCCAG GATTCCCAAA TGGCTACCCT TCCTACACAC ACTGCATCTG GAGAGTGTCT 
GTGACCCCGG GAGAAAAGAT TGTCTTGAAT TTTACCACAA TGGACCTTTA CAAAAGTAGT 
TTGTGCTGGT ATGATTACAT TGAAGTAAGA GATGGTTACT GGAGGAAGTC ACCTCTCCTT 
GGTAGATTCT GTGGGGACAA AGTGGCTGGA GTTCTTACAT CTACGGACAG CAGAATGTGG 
ATTGAGTTTC GTAGCAGCAG TAACTGGGTA GGAAAAGGGT TTGCAGCTGT CTATGAAGCG 
ATTTGTGGAG GGGAGATAAG GAAAAACGAA GGGCAGATTC AGTCTCCCAA TTACCCCGAT 
GACTACCGAC CAATGAAGGA GTGTGTATGG AAAATAATGG TGTCCGAGGG CTACCATGTT 
GGACTGACCT TTCAGGCCTT TGAGATCGAA AGACATGACA GCTGTGCCTA TGACCACCTA 
GAAGTTCGAG ATGGAGCCAG TGAGAACAGC CCTTTGATAG GACGGTTCTG TGGTTATGAC 
AAACCTGAAG ATATAAGGTC TACTTCCAAC ACCCTGTGGA TGAAGTTTGT CTCTGACGGG 
ACTGTGAACA AGGCAGGGTT TGCTGCGAAC TTTTTTAAAG AGGAAGATGA GTGTGCCAAA 
CCTGACCGAG GAGGCTGTGA ACAGAGGTGT CTTAACACAC TAGGCAGCTA CCAGTGTGCC 
TGTGAGCCTG GCTATGAACT GGGGCCAGAC AGAAGAAGCT GTGAAGCTGC TTGCGGAGGA 
CTTCTGACGA AGCTCAATGG CACCATAACC ACCCCCGGCT GGCCCAAAGA GTACCCTCCA 
AACAAAAACT GTGTGTGGCA AGTGATCGCG CCAAGCCAGT ACAGAATCTC TGTGAAGTTT 
GAGTTTTTTG AATTGGAAGG CAATGAAGTT TGCAAATACG ATTACGTGGA GATCTGGAGC 
GGCCTTTCCT CTGAGTCTAA ACTGCATGGC AAGTTCTGTG GCGCTGACAT ACCTGAAGTG 
ATGACTTCCC ATTTCAACAA CATGAGGATT GAATTCAAGT CAGACAACAC TGTATCCAAG 
AAGGGCTTCA AAGCACATTT TTTCTCAGAT AAGGATGAGT GTTCAAAGGA TAATGGTGGC 
TGTCAGCATG AGTGTGTCAA CACGATGGGA AGTTACACGT GTCAGTGCCG GAATGGATTC 
GTGTTGCATG AGAACAAGCA TGATTGCAAG GAAGCCGAGT GTGAACAGAA GATACACAGC 
CCAAGTGGTC TCATCACCAG TCCCAACTGG CCAGACAAGT ATCCAAGCAG GAAAGAGTGC 
ACGTGGGTGA TCAGTGCCAT TCCTGGCCAC CGCATCACAT TAGCCTTCAA TGAGTTTGAG 
GTTGAACAAC ATCAAGAATG TGCTTATGAT CACTTGGAAA TTTTTGATGG AGAAACGGAG 
AAGTCACCAA TACTTGGCCG ACTATGTGGC AGCAAGATAC CAGATCCCCT CATGGCTACT 
GGGAATGAAA TGTTTATTCG GTTTATTTCT GATGCCTCTG TTCAAAGAAA AGGCTTTCAA 
GCTACACATT CCACAGAGTG TGGTGGTCGA TTGAAAGCAG AGTCAAAGCC TAGAGACCTG 
TACTCCCATG CTCAGTTTGG TGATAATAAC TACCCAGGAC AACTGGACTG TGAATGGTTG 
TTGGTGTCAG AACGAGGATC TCGACTTGAA TTGTCCTTCC AGACATTCGA AGTAGAAGAA 
GAAGCTGACT GTGGCTATGA CTATGTTGAA GTCTTTGATG GTCTCAGTTC AAAAGCTGTG 
GGTCTTGGTA GATTCTGTGG GTCAGGGCCA CCAGAAGAAA TCTATTCAAT TGGAGATGTG 
GCTTTGATTC ATTTCCACAC AGATGACACT ATCAACAAGA AAGGATTTTA TATAAGATAT 
AAAAGTATAA GATACCCGGA AACGATGCAT GCCAAGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inactivation of bone morphogenetic protein 1 (Bmp1) and tolloid-like 1 (Tll1) in cells expressing type I collagen leads to dental and periodontal defects in mice.
Journal of molecular histology48(2)83-98(2017 Apr)
Zhang H,Jani P,Liang T,Lu Y,Qin C

Essential Roles of Bone Morphogenetic Protein-1 and Mammalian Tolloid-like 1 in Postnatal Root Dentin Formation.
Journal of endodontics43(1)109-115(2017 Jan)
Wang J,Muir AM,Ren Y,Massoudi D,Greenspan DS,Feng JQ

BMP1-like proteinases are essential to the structure and wound healing of skin.
Matrix biology : journal of the International Society for Matrix Biology56114-131(2016 Dec)
Muir AM,Massoudi D,Nguyen N,Keene DR,Lee SJ,Birk DE,Davidson JM,Marinkovich MP,Greenspan DS

Mammalian tolloid proteinases: role in growth factor signalling.
FEBS letters590(15)2398-407(2016 08)
Troilo H,Bayley CP,Barrett AL,Lockhart-Cairns MP,Jowitt TA,Baldock C

Gene network and familial analyses uncover a gene network involving Tbx5/Osr1/Pcsk6 interaction in the second heart field for atrial septation.
Human molecular genetics25(6)1140-51(2016 Mar)
Zhang KK,Xiang M,Zhou L,Liu J,Curry N,Heine Su?er D,Garcia-Pavia P,Zhang X,Wang Q,Xie L

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Tll1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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