tok cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tok
Entrez Gene ID	42944
Full Name	tolkin
Synonyms	CG6863,CT21251,Dmel\CG6863,TOK,Tlr,Tlr-1,Tok,tldr-1,tldr1,tlr,tlr-1,tlr1
General protein information	Preferred Names tolkin Names CG6863 gene product from transcript CG6863-RB CG6863-PA CG6863-PB CG6863-PC Tolloid-related piranha tld-related-1 tok-PA tok-PB tok-PC tolloid-related 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96A18-96A19

mRNA and Protein(s)

mRNA	Protein	Name
NM_057531.4	NP_476879.1	tolkin, isoform A
NM_001300581.1	NP_001287510.1	tolkin, isoform C
NM_170168.2	NP_733035.1	tolkin, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	TLL1	NP_036596.3
	TLL1	XP_001101883.2
Gallus gallus (chicken)	TLL1	NP_990034.1
Danio rerio (zebrafish)	tll1	NP_571085.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003702	XP_313494.5
Caenorhabditis elegans (roundworm)	nas-39	NP_510672.2
Drosophila melanogaster (fruit fly)	tok	NP_733035.1
Bos taurus (cattle)	TLL1	NP_001180043.1
Mus musculus (house mouse)	Tll1	NP_033416.2
Canis lupus familiaris (dog)	TLL1	XP_539791.2
Pan troglodytes (chimpanzee)	TLL1	XP_003310613.1
Rattus norvegicus (Norway rat)	Tll1	NP_001099551.1
Xenopus tropicalis (tropical clawed frog)	LOC100495742	XP_004911222.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study suggests that by activating Daw and perhaps other TGF-beta ligands, tolloid related provides a permissive signal for axon guidance
Title: The metalloprotease tolloid-related and its TGF-beta-like substrate Dawdle regulate Drosophila motoneuron axon guidance.

The following tok gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tok cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06006	NM_057531.4 Latest version!	Drosophila melanogaster tolkin (tok), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa06006	NM_170168.2 Latest version!	Drosophila melanogaster tolkin (tok), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa06006	NM_001300581.1 Latest version!	Drosophila melanogaster tolkin (tok), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tok ( NM_057531.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476879.1 Drosophila melanogaster tolkin (tok), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06006
Clone ID Related Accession (Same CDS sequence)	NM_057531.4 , NM_001300581.1 , NM_170168.2
Accession Version	NM_057531.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tolkin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057531.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTAGGA GGCGTGGCAC GCCCCTGGGC GTCAGCTGGA CGAACTGCGT CCTGCTCCTG 
GCCACCGGTC TGCTGGTGGT GCTCATTTCG GTGCACGCCA AGAACCCCAG GCCGCATCGA 
GGACTACCCG CCCCGGCTCC TGCACCAGTT CCTGCATCCG TAGATCAGGT GGCAACTGAA 
AATGCCCACA GGCGGGAACC TACCGTCGAT GGGAGTGTAC TTCAACGGCT ATCGGGTTCA 
GATAGGGCTG TTGACGAGGA TGAGGATGTC GATGAGGATG AGGATGAGGG GCTAAAGCAC 
CAGCACCTCG AGCATCGGCA ACAATTTGAC GCATATCTGG GCACAATCCG ACGGCTGCGT 
CGTCCATACC GCAGCAAATA CACCATCGAG CAGCTGATGC GACTGCATGT GACACCAAAG 
GTATCCGGAG ACATTGACAT GGATCCCTGC AAGGCGGGCG GTTTCATGGG CGACATTGCC 
CTGCCGGAGG GTGATTCCGG CCCGGTGGTT GCCCACACGC CCATCGACGA CGAGGAGCAG 
GAGCGCCAGG AGGTGGAGCA GCTGCTGGCT GACTCGCCCA GCCCGGTTCA CGACCCCCCT 
TCCGTTTCCG GTGCGGCCAA CTCAGGGCCG ACCAAGTTCA ATGCCACCTT CCAGCTGGAT 
CTGGAGAAGC TCAAGCAGGA GGTCTACCAC GAGGGCCTAC AGGTGGAGGA GGAGGGCCTC 
ACCGACATCA TCCGCAAGAA GACCAAGCTG CCCAGCTCAG GTCCGGTCAA CCTGAAGGCA 
GGACAAAGCC AGTCACAGAG TCAGAGTGCC AATGAACCCG TGAAGCCCGG TTCGGAATCG 
CCCTCCGATT ACGCGCAAGC ATCCAGCTAC AAGAATGAGG TACTGCCTCC GTCGGAGCAG 
CGAATCCATA CGGACAAGCC GGCACGGTTG ACGCTGGCCG AGATTGGACC CAAGTTCAGC 
AACAAGACGC AGTCGCTGCA CCGTAATGTG GACACACGCA AGATTCAGTC GGACAACAAG 
GACATCGAGG ACGATTCGGG CAACAATTTT GTGGCCGAGA GGAGGCCGTC ACAGATTGTG 
CCCACTTCCT TGCCGGAGCA GCGATCCACT CCGGCTCACG TGTACAGCAA TGCCGGAGGC 
AATGGCATGC AGTTGGACGA CGAGGTGGAC TTCTTGCATT CCCGTGGAGT CGTGAGGAGG 
CGTCACAGGC GAGGACCAAA ACAGCGAAGG AGCAACCAGC ACTTGGAGCA CGAGGTCAAG 
CTGCAGAGAT TGAGGGAGGA GCTCAGCCGT CCGGTCATCG AGGCCCACCA TCACCAGTCG 
CACCACCAGA AGCAGGCGCA GCACCGCCAG CACCACCAGA GCCACCATCG AAAGAAGCAC 
CGTCGACAGC AGCAGCACCA TCGTCGACGT GGCCAGAGCA CCACGCAATA TGTGAACCAT 
TCCCCGCCAG GATTCAATCA ATCGCAGCAG GCCCAACAGC AGTCAGTGCC TGAAATGGAT 
TTGTTGAAAG CGGAAACGTC CAAGGATGAG GAGCCACTGC GTCATCGGGT GGCCCGTGCT 
GTCACCGCCA AAAAGGAACG CATCTGGGAC TATGGGGTGA TTCCGTACGA GATCGATGGC 
AACTTCAGTG GCATCCATAA GGCGCTTTTC AAACTGGCCA TGCGCCATTG GGAGAACTCC 
ACGTGTATCA AGTTCGTAGA GCGCGACCCG GAGATCCATC CCAATTACAT AGTGTTCACG 
GTTCGCAGCT GCGGATGCTG CTCATTTGTG GGCAAACGTG GAAATGGACC GCAGGCCATC 
TCAATTGGTA GAAATTGCGA TAAATTTGGC ATTGTGGTCC ATGAGTTGGG TCACGTGGTG 
GGCTTCTGGC ACGAGCACAC GCGTCCGGAT CGGGAAAAGC ACGTGGTGAT CGAGCATAAC 
AACATTATGA AGGGCCAGGA CTATAACTTC AATATGCTAT CCCCCGATGA AGTGGACTCG 
CTGGGCATGG CCTACGACTA TGATTCGATT ATGCACTACG CACGGAACAC CTTCTCCAAG 
GGCACCTACC TGGACACCAT CCTGCCCATC GAGATGAAGG GTCGCAAGCG TCCTGAAATA 
GGCCAGCGGT TGCGCCTCAG CCAGGGTGAC ATTGCGCAGG CGAATCTTCT GTACAAGTGT 
CCAAAGTGCG GAAGAACCTT TCAGGAGAGT TCCGGCATCT TTGCCTCACC ATCTCATTAC 
ACGGCCGGAG CTTTGAGCAA TGAGACGGAG CACTGCGAGT GGCGGATTAC AGCGACATAC 
GGAGAAAGAG TGGAACTGAA ACTGGAGAAT ATGAACATCT TTAAGTCAAA CAACTGCGAG 
ACGGACTACC TAGAGATTCG GGATGGCTAC TTCGAGAAGT CACCGCTTAT CGGACGCTTC 
TGCGGCAAGG TGGACAAGGA GGTGATCCGC ACGGAGTCCA GTCGCATGCT GCTCACCTAT 
GTGAATACAC ACCGCATCGA GGGCTTCAGG GGCTTCAAGG CGGAGTTCGA TGTTGTTTGC 
GGTGGCGAGT TGTCCGTGGA CGATGCCGTT GGCCGTTTGG AGTCGCCCAA CTATCCACTG 
GACTATTTGC CCAACAAGGA ATGTGTGTGG AAAATAACTG TGCCCGAAAG CTACCAGGTG 
GCGTTGAAAT TCCAGTCCTT CGAAGTGGAG AACCACGACA GCTGTGTCTA CGACTACGTC 
GAGGTCCGGG ACGGTCCTGG CCAGGATGCG CCGCTAATTG GCGTTTTCTG TGGCTACAAG 
CCGCCGCCGA ACATGAAGTC AAGTGGCAAT TCGATGTACG TGAAATTCGT GTCGGACACA 
TCGGTGCAGA AGGCGGGCTT CTCGGCCGTG TTCATGAAGG AGGTGGACGA GTGCGAGACC 
CAGAACCACG GCTGCGAGCA CGAATGCATC AACACCCTCG GTGGCTACGA GTGCAGCTGC 
CGCATCGGAT TCGAGCTCCA CAGCGACAAG AAGCACTGCG AAGATGCCTG TGGCGGTGTC 
ATCGAGTATC CGAATGGCAC CATTACCTCG CCCTCGTTCC CCGAGATGTA CCCTCTGCTG 
AAGGAGTGCA TCTGGGAGAT TGTGGCGCCA CCGAAGCACA GGATATCGCT AAACTTCACC 
CACTTCGACC TTGAGGGCAC GGCGCATCAG CAATCGGACT GTGGCTACGA TTCGGTCACA 
GTCTACTCAA AACTGGGCGA GAATCGTCTG AAGAGGATCG GCACCTTCTG CGGCTCCAGC 
ATTCCGCCTA CGGCCACCTC CGAGAGTAAT GCGTTGAGAC TGGAGTTCCA TTCGGACAAG 
TCCATTCAGA GATCAGGCTT CGCGGCCGTC TTCTTCACGG ACATCGACGA GTGTGCCGTG 
AATAATGGTG GTTGTCAGCA TGAGTGCCGG AACACGATTG GATCCTACAT CTGCATGTGC 
CACAACGGGT ACTCCATGCA CGAGAATGGC CACGACTGCA AGGAGGGCGA GTGCAAGTAC 
GAGATCTCCG CCCCCTTCGG CACGATTTTC AGTCCCAATT ACCCGGATTC CTATCCCCCA 
AATGCGGACT GCGTTTGGCA CTTCATAACC ACGCCGGGGC ACCGCATCAA GCTGATTTTC 
AACGAGTTCG ACGTGGAATC GCATCAGGAA TGCACCTACG ACAACGTGGC AGTTTACGAT 
GGCGAATCTG AGTCCAGTTC GGTCCTGGGT CGCTTCTGCG GGGATAAGAT TCCCTTCCCC 
ATCTCCTCCA CCAGCAACCA GATGTACATG GTGCTCAAGA CGGACAAGAA CAAGCAAAAG 
AACGGCTTCA CAGCCTCCCA CTCCACGGCC TGTGGGGGCT ACTTGCGTGC CACTAGCCAG 
GTGCAGCAGT TCTATTCGCA CGCCCGATTC GGAAACCAGG ACTATGACGA CGGCATGGAC 
TGCGAGTGGA CCATCGCGGC GCCGGACAAC AGCTACGTCC AGCTCATATT CCTCACCTTC 
GACATCGAGA GCAGCGAGAA CTGCACCTTT GACTACGTGC AGGTGTTCTC TGACATAGAC 
GATGTGTACG GACAGTATGG ACCCATGTAC GGACAGTACT GTGGCAATGT GCTGCCGCAG 
GACATCAACT CTATGACACA TTCTCTGCTG GTTCGCTTTA AGACCGACGG TTCGGTGCCG 
ATGAAGGGCT TCTCTGCCTC ATATGTGGCC GTGCCCAATT CCGGGGAGTA TGACCACTCC 
GACGAGGATG TGGAGAACTC GTACAGCTCC GAGATGGTTA CCCCGTTTCC GGGCTCACTG 
AAGAGCATCT ACATCGAGGA CACGCAGGAG GAGACCGACG AGTACAGCGA CTTCAGCGGC 
AATCAACTGG TCTTCAATGG GCAGTATCGT GGCCGATATA GTCTGCCCAA ACGGTACTAC 
TCCGGAAAGG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476879.1
CDS	329..4723
Translation	MRRRRGTPLGVSWTNCVLLLATGLLVVLISVHAKNPRPHRGLPAPAPAPVPASVDQVATENAHRREPTVDGSVLQRLSGSDRAVDEDEDVDEDEDEGLKHQHLEHRQQFDAYLGTIRRLRRPYRSKYTIEQLMRLHVTPKVSGDIDMDPCKAGGFMGDIALPEGDSGPVVAHTPIDDEEQERQEVEQLLADSPSPVHDPPSVSGAANSGPTKFNATFQLDLEKLKQEVYHEGLQVEEEGLTDIIRKKTKLPSSGPVNLKAGQSQSQSQSANEPVKPGSESPSDYAQASSYKNEVLPPSEQRIHTDKPARLTLAEIGPKFSNKTQSLHRNVDTRKIQSDNKDIEDDSGNNFVAERRPSQIVPTSLPEQRSTPAHVYSNAGGNGMQLDDEVDFLHSRGVVRRRHRRGPKQRRSNQHLEHEVKLQRLREELSRPVIEAHHHQSHHQKQAQHRQHHQSHHRKKHRRQQQHHRRRGQSTTQYVNHSPPGFNQSQQAQQQSVPEMDLLKAETSKDEEPLRHRVARAVTAKKERIWDYGVIPYEIDGNFSGIHKALFKLAMRHWENSTCIKFVERDPEIHPNYIVFTVRSCGCCSFVGKRGNGPQAISIGRNCDKFGIVVHELGHVVGFWHEHTRPDREKHVVIEHNNIMKGQDYNFNMLSPDEVDSLGMAYDYDSIMHYARNTFSKGTYLDTILPIEMKGRKRPEIGQRLRLSQGDIAQANLLYKCPKCGRTFQESSGIFASPSHYTAGALSNETEHCEWRITATYGERVELKLENMNIFKSNNCETDYLEIRDGYFEKSPLIGRFCGKVDKEVIRTESSRMLLTYVNTHRIEGFRGFKAEFDVVCGGELSVDDAVGRLESPNYPLDYLPNKECVWKITVPESYQVALKFQSFEVENHDSCVYDYVEVRDGPGQDAPLIGVFCGYKPPPNMKSSGNSMYVKFVSDTSVQKAGFSAVFMKEVDECETQNHGCEHECINTLGGYECSCRIGFELHSDKKHCEDACGGVIEYPNGTITSPSFPEMYPLLKECIWEIVAPPKHRISLNFTHFDLEGTAHQQSDCGYDSVTVYSKLGENRLKRIGTFCGSSIPPTATSESNALRLEFHSDKSIQRSGFAAVFFTDIDECAVNNGGCQHECRNTIGSYICMCHNGYSMHENGHDCKEGECKYEISAPFGTIFSPNYPDSYPPNADCVWHFITTPGHRIKLIFNEFDVESHQECTYDNVAVYDGESESSSVLGRFCGDKIPFPISSTSNQMYMVLKTDKNKQKNGFTASHSTACGGYLRATSQVQQFYSHARFGNQDYDDGMDCEWTIAAPDNSYVQLIFLTFDIESSENCTFDYVQVFSDIDDVYGQYGPMYGQYCGNVLPQDINSMTHSLLVRFKTDGSVPMKGFSASYVAVPNSGEYDHSDEDVENSYSSEMVTPFPGSLKSIYIEDTQEETDEYSDFSGNQLVFNGQYRGRYSLPKRYYSGKA

Target ORF information:

RefSeq Version	NM_057531.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tolkin (tok), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057531.4

ORF Insert Sequence:

ATGCGTAGGA GGCGTGGCAC GCCCCTGGGC GTCAGCTGGA CGAACTGCGT CCTGCTCCTG 
GCCACCGGTC TGCTGGTGGT GCTCATTTCG GTGCACGCCA AGAACCCCAG GCCGCATCGA 
GGACTACCCG CCCCGGCTCC TGCACCAGTT CCTGCATCCG TAGATCAGGT GGCAACTGAA 
AATGCCCACA GGCGGGAACC TACCGTCGAT GGGAGTGTAC TTCAACGGCT ATCGGGTTCA 
GATAGGGCTG TTGACGAGGA TGAGGATGTC GATGAGGATG AGGATGAGGG GCTAAAGCAC 
CAGCACCTCG AGCATCGGCA ACAATTTGAC GCATATCTGG GCACAATCCG ACGGCTGCGT 
CGTCCATACC GCAGCAAATA CACCATCGAG CAGCTGATGC GACTGCATGT GACACCAAAG 
GTATCCGGAG ACATTGACAT GGATCCCTGC AAGGCGGGCG GTTTCATGGG CGACATTGCC 
CTGCCGGAGG GTGATTCCGG CCCGGTGGTT GCCCACACGC CCATCGACGA CGAGGAGCAG 
GAGCGCCAGG AGGTGGAGCA GCTGCTGGCT GACTCGCCCA GCCCGGTTCA CGACCCCCCT 
TCCGTTTCCG GTGCGGCCAA CTCAGGGCCG ACCAAGTTCA ATGCCACCTT CCAGCTGGAT 
CTGGAGAAGC TCAAGCAGGA GGTCTACCAC GAGGGCCTAC AGGTGGAGGA GGAGGGCCTC 
ACCGACATCA TCCGCAAGAA GACCAAGCTG CCCAGCTCAG GTCCGGTCAA CCTGAAGGCA 
GGACAAAGCC AGTCACAGAG TCAGAGTGCC AATGAACCCG TGAAGCCCGG TTCGGAATCG 
CCCTCCGATT ACGCGCAAGC ATCCAGCTAC AAGAATGAGG TACTGCCTCC GTCGGAGCAG 
CGAATCCATA CGGACAAGCC GGCACGGTTG ACGCTGGCCG AGATTGGACC CAAGTTCAGC 
AACAAGACGC AGTCGCTGCA CCGTAATGTG GACACACGCA AGATTCAGTC GGACAACAAG 
GACATCGAGG ACGATTCGGG CAACAATTTT GTGGCCGAGA GGAGGCCGTC ACAGATTGTG 
CCCACTTCCT TGCCGGAGCA GCGATCCACT CCGGCTCACG TGTACAGCAA TGCCGGAGGC 
AATGGCATGC AGTTGGACGA CGAGGTGGAC TTCTTGCATT CCCGTGGAGT CGTGAGGAGG 
CGTCACAGGC GAGGACCAAA ACAGCGAAGG AGCAACCAGC ACTTGGAGCA CGAGGTCAAG 
CTGCAGAGAT TGAGGGAGGA GCTCAGCCGT CCGGTCATCG AGGCCCACCA TCACCAGTCG 
CACCACCAGA AGCAGGCGCA GCACCGCCAG CACCACCAGA GCCACCATCG AAAGAAGCAC 
CGTCGACAGC AGCAGCACCA TCGTCGACGT GGCCAGAGCA CCACGCAATA TGTGAACCAT 
TCCCCGCCAG GATTCAATCA ATCGCAGCAG GCCCAACAGC AGTCAGTGCC TGAAATGGAT 
TTGTTGAAAG CGGAAACGTC CAAGGATGAG GAGCCACTGC GTCATCGGGT GGCCCGTGCT 
GTCACCGCCA AAAAGGAACG CATCTGGGAC TATGGGGTGA TTCCGTACGA GATCGATGGC 
AACTTCAGTG GCATCCATAA GGCGCTTTTC AAACTGGCCA TGCGCCATTG GGAGAACTCC 
ACGTGTATCA AGTTCGTAGA GCGCGACCCG GAGATCCATC CCAATTACAT AGTGTTCACG 
GTTCGCAGCT GCGGATGCTG CTCATTTGTG GGCAAACGTG GAAATGGACC GCAGGCCATC 
TCAATTGGTA GAAATTGCGA TAAATTTGGC ATTGTGGTCC ATGAGTTGGG TCACGTGGTG 
GGCTTCTGGC ACGAGCACAC GCGTCCGGAT CGGGAAAAGC ACGTGGTGAT CGAGCATAAC 
AACATTATGA AGGGCCAGGA CTATAACTTC AATATGCTAT CCCCCGATGA AGTGGACTCG 
CTGGGCATGG CCTACGACTA TGATTCGATT ATGCACTACG CACGGAACAC CTTCTCCAAG 
GGCACCTACC TGGACACCAT CCTGCCCATC GAGATGAAGG GTCGCAAGCG TCCTGAAATA 
GGCCAGCGGT TGCGCCTCAG CCAGGGTGAC ATTGCGCAGG CGAATCTTCT GTACAAGTGT 
CCAAAGTGCG GAAGAACCTT TCAGGAGAGT TCCGGCATCT TTGCCTCACC ATCTCATTAC 
ACGGCCGGAG CTTTGAGCAA TGAGACGGAG CACTGCGAGT GGCGGATTAC AGCGACATAC 
GGAGAAAGAG TGGAACTGAA ACTGGAGAAT ATGAACATCT TTAAGTCAAA CAACTGCGAG 
ACGGACTACC TAGAGATTCG GGATGGCTAC TTCGAGAAGT CACCGCTTAT CGGACGCTTC 
TGCGGCAAGG TGGACAAGGA GGTGATCCGC ACGGAGTCCA GTCGCATGCT GCTCACCTAT 
GTGAATACAC ACCGCATCGA GGGCTTCAGG GGCTTCAAGG CGGAGTTCGA TGTTGTTTGC 
GGTGGCGAGT TGTCCGTGGA CGATGCCGTT GGCCGTTTGG AGTCGCCCAA CTATCCACTG 
GACTATTTGC CCAACAAGGA ATGTGTGTGG AAAATAACTG TGCCCGAAAG CTACCAGGTG 
GCGTTGAAAT TCCAGTCCTT CGAAGTGGAG AACCACGACA GCTGTGTCTA CGACTACGTC 
GAGGTCCGGG ACGGTCCTGG CCAGGATGCG CCGCTAATTG GCGTTTTCTG TGGCTACAAG 
CCGCCGCCGA ACATGAAGTC AAGTGGCAAT TCGATGTACG TGAAATTCGT GTCGGACACA 
TCGGTGCAGA AGGCGGGCTT CTCGGCCGTG TTCATGAAGG AGGTGGACGA GTGCGAGACC 
CAGAACCACG GCTGCGAGCA CGAATGCATC AACACCCTCG GTGGCTACGA GTGCAGCTGC 
CGCATCGGAT TCGAGCTCCA CAGCGACAAG AAGCACTGCG AAGATGCCTG TGGCGGTGTC 
ATCGAGTATC CGAATGGCAC CATTACCTCG CCCTCGTTCC CCGAGATGTA CCCTCTGCTG 
AAGGAGTGCA TCTGGGAGAT TGTGGCGCCA CCGAAGCACA GGATATCGCT AAACTTCACC 
CACTTCGACC TTGAGGGCAC GGCGCATCAG CAATCGGACT GTGGCTACGA TTCGGTCACA 
GTCTACTCAA AACTGGGCGA GAATCGTCTG AAGAGGATCG GCACCTTCTG CGGCTCCAGC 
ATTCCGCCTA CGGCCACCTC CGAGAGTAAT GCGTTGAGAC TGGAGTTCCA TTCGGACAAG 
TCCATTCAGA GATCAGGCTT CGCGGCCGTC TTCTTCACGG ACATCGACGA GTGTGCCGTG 
AATAATGGTG GTTGTCAGCA TGAGTGCCGG AACACGATTG GATCCTACAT CTGCATGTGC 
CACAACGGGT ACTCCATGCA CGAGAATGGC CACGACTGCA AGGAGGGCGA GTGCAAGTAC 
GAGATCTCCG CCCCCTTCGG CACGATTTTC AGTCCCAATT ACCCGGATTC CTATCCCCCA 
AATGCGGACT GCGTTTGGCA CTTCATAACC ACGCCGGGGC ACCGCATCAA GCTGATTTTC 
AACGAGTTCG ACGTGGAATC GCATCAGGAA TGCACCTACG ACAACGTGGC AGTTTACGAT 
GGCGAATCTG AGTCCAGTTC GGTCCTGGGT CGCTTCTGCG GGGATAAGAT TCCCTTCCCC 
ATCTCCTCCA CCAGCAACCA GATGTACATG GTGCTCAAGA CGGACAAGAA CAAGCAAAAG 
AACGGCTTCA CAGCCTCCCA CTCCACGGCC TGTGGGGGCT ACTTGCGTGC CACTAGCCAG 
GTGCAGCAGT TCTATTCGCA CGCCCGATTC GGAAACCAGG ACTATGACGA CGGCATGGAC 
TGCGAGTGGA CCATCGCGGC GCCGGACAAC AGCTACGTCC AGCTCATATT CCTCACCTTC 
GACATCGAGA GCAGCGAGAA CTGCACCTTT GACTACGTGC AGGTGTTCTC TGACATAGAC 
GATGTGTACG GACAGTATGG ACCCATGTAC GGACAGTACT GTGGCAATGT GCTGCCGCAG 
GACATCAACT CTATGACACA TTCTCTGCTG GTTCGCTTTA AGACCGACGG TTCGGTGCCG 
ATGAAGGGCT TCTCTGCCTC ATATGTGGCC GTGCCCAATT CCGGGGAGTA TGACCACTCC 
GACGAGGATG TGGAGAACTC GTACAGCTCC GAGATGGTTA CCCCGTTTCC GGGCTCACTG 
AAGAGCATCT ACATCGAGGA CACGCAGGAG GAGACCGACG AGTACAGCGA CTTCAGCGGC 
AATCAACTGG TCTTCAATGG GCAGTATCGT GGCCGATATA GTCTGCCCAA ACGGTACTAC 
TCCGGAAAGG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tok ( NM_001300581.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287510.1 Drosophila melanogaster tolkin (tok), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa06006
Clone ID Related Accession (Same CDS sequence)	NM_057531.4 , NM_001300581.1 , NM_170168.2
Accession Version	NM_001300581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tolkin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTAGGA GGCGTGGCAC GCCCCTGGGC GTCAGCTGGA CGAACTGCGT CCTGCTCCTG 
GCCACCGGTC TGCTGGTGGT GCTCATTTCG GTGCACGCCA AGAACCCCAG GCCGCATCGA 
GGACTACCCG CCCCGGCTCC TGCACCAGTT CCTGCATCCG TAGATCAGGT GGCAACTGAA 
AATGCCCACA GGCGGGAACC TACCGTCGAT GGGAGTGTAC TTCAACGGCT ATCGGGTTCA 
GATAGGGCTG TTGACGAGGA TGAGGATGTC GATGAGGATG AGGATGAGGG GCTAAAGCAC 
CAGCACCTCG AGCATCGGCA ACAATTTGAC GCATATCTGG GCACAATCCG ACGGCTGCGT 
CGTCCATACC GCAGCAAATA CACCATCGAG CAGCTGATGC GACTGCATGT GACACCAAAG 
GTATCCGGAG ACATTGACAT GGATCCCTGC AAGGCGGGCG GTTTCATGGG CGACATTGCC 
CTGCCGGAGG GTGATTCCGG CCCGGTGGTT GCCCACACGC CCATCGACGA CGAGGAGCAG 
GAGCGCCAGG AGGTGGAGCA GCTGCTGGCT GACTCGCCCA GCCCGGTTCA CGACCCCCCT 
TCCGTTTCCG GTGCGGCCAA CTCAGGGCCG ACCAAGTTCA ATGCCACCTT CCAGCTGGAT 
CTGGAGAAGC TCAAGCAGGA GGTCTACCAC GAGGGCCTAC AGGTGGAGGA GGAGGGCCTC 
ACCGACATCA TCCGCAAGAA GACCAAGCTG CCCAGCTCAG GTCCGGTCAA CCTGAAGGCA 
GGACAAAGCC AGTCACAGAG TCAGAGTGCC AATGAACCCG TGAAGCCCGG TTCGGAATCG 
CCCTCCGATT ACGCGCAAGC ATCCAGCTAC AAGAATGAGG TACTGCCTCC GTCGGAGCAG 
CGAATCCATA CGGACAAGCC GGCACGGTTG ACGCTGGCCG AGATTGGACC CAAGTTCAGC 
AACAAGACGC AGTCGCTGCA CCGTAATGTG GACACACGCA AGATTCAGTC GGACAACAAG 
GACATCGAGG ACGATTCGGG CAACAATTTT GTGGCCGAGA GGAGGCCGTC ACAGATTGTG 
CCCACTTCCT TGCCGGAGCA GCGATCCACT CCGGCTCACG TGTACAGCAA TGCCGGAGGC 
AATGGCATGC AGTTGGACGA CGAGGTGGAC TTCTTGCATT CCCGTGGAGT CGTGAGGAGG 
CGTCACAGGC GAGGACCAAA ACAGCGAAGG AGCAACCAGC ACTTGGAGCA CGAGGTCAAG 
CTGCAGAGAT TGAGGGAGGA GCTCAGCCGT CCGGTCATCG AGGCCCACCA TCACCAGTCG 
CACCACCAGA AGCAGGCGCA GCACCGCCAG CACCACCAGA GCCACCATCG AAAGAAGCAC 
CGTCGACAGC AGCAGCACCA TCGTCGACGT GGCCAGAGCA CCACGCAATA TGTGAACCAT 
TCCCCGCCAG GATTCAATCA ATCGCAGCAG GCCCAACAGC AGTCAGTGCC TGAAATGGAT 
TTGTTGAAAG CGGAAACGTC CAAGGATGAG GAGCCACTGC GTCATCGGGT GGCCCGTGCT 
GTCACCGCCA AAAAGGAACG CATCTGGGAC TATGGGGTGA TTCCGTACGA GATCGATGGC 
AACTTCAGTG GCATCCATAA GGCGCTTTTC AAACTGGCCA TGCGCCATTG GGAGAACTCC 
ACGTGTATCA AGTTCGTAGA GCGCGACCCG GAGATCCATC CCAATTACAT AGTGTTCACG 
GTTCGCAGCT GCGGATGCTG CTCATTTGTG GGCAAACGTG GAAATGGACC GCAGGCCATC 
TCAATTGGTA GAAATTGCGA TAAATTTGGC ATTGTGGTCC ATGAGTTGGG TCACGTGGTG 
GGCTTCTGGC ACGAGCACAC GCGTCCGGAT CGGGAAAAGC ACGTGGTGAT CGAGCATAAC 
AACATTATGA AGGGCCAGGA CTATAACTTC AATATGCTAT CCCCCGATGA AGTGGACTCG 
CTGGGCATGG CCTACGACTA TGATTCGATT ATGCACTACG CACGGAACAC CTTCTCCAAG 
GGCACCTACC TGGACACCAT CCTGCCCATC GAGATGAAGG GTCGCAAGCG TCCTGAAATA 
GGCCAGCGGT TGCGCCTCAG CCAGGGTGAC ATTGCGCAGG CGAATCTTCT GTACAAGTGT 
CCAAAGTGCG GAAGAACCTT TCAGGAGAGT TCCGGCATCT TTGCCTCACC ATCTCATTAC 
ACGGCCGGAG CTTTGAGCAA TGAGACGGAG CACTGCGAGT GGCGGATTAC AGCGACATAC 
GGAGAAAGAG TGGAACTGAA ACTGGAGAAT ATGAACATCT TTAAGTCAAA CAACTGCGAG 
ACGGACTACC TAGAGATTCG GGATGGCTAC TTCGAGAAGT CACCGCTTAT CGGACGCTTC 
TGCGGCAAGG TGGACAAGGA GGTGATCCGC ACGGAGTCCA GTCGCATGCT GCTCACCTAT 
GTGAATACAC ACCGCATCGA GGGCTTCAGG GGCTTCAAGG CGGAGTTCGA TGTTGTTTGC 
GGTGGCGAGT TGTCCGTGGA CGATGCCGTT GGCCGTTTGG AGTCGCCCAA CTATCCACTG 
GACTATTTGC CCAACAAGGA ATGTGTGTGG AAAATAACTG TGCCCGAAAG CTACCAGGTG 
GCGTTGAAAT TCCAGTCCTT CGAAGTGGAG AACCACGACA GCTGTGTCTA CGACTACGTC 
GAGGTCCGGG ACGGTCCTGG CCAGGATGCG CCGCTAATTG GCGTTTTCTG TGGCTACAAG 
CCGCCGCCGA ACATGAAGTC AAGTGGCAAT TCGATGTACG TGAAATTCGT GTCGGACACA 
TCGGTGCAGA AGGCGGGCTT CTCGGCCGTG TTCATGAAGG AGGTGGACGA GTGCGAGACC 
CAGAACCACG GCTGCGAGCA CGAATGCATC AACACCCTCG GTGGCTACGA GTGCAGCTGC 
CGCATCGGAT TCGAGCTCCA CAGCGACAAG AAGCACTGCG AAGATGCCTG TGGCGGTGTC 
ATCGAGTATC CGAATGGCAC CATTACCTCG CCCTCGTTCC CCGAGATGTA CCCTCTGCTG 
AAGGAGTGCA TCTGGGAGAT TGTGGCGCCA CCGAAGCACA GGATATCGCT AAACTTCACC 
CACTTCGACC TTGAGGGCAC GGCGCATCAG CAATCGGACT GTGGCTACGA TTCGGTCACA 
GTCTACTCAA AACTGGGCGA GAATCGTCTG AAGAGGATCG GCACCTTCTG CGGCTCCAGC 
ATTCCGCCTA CGGCCACCTC CGAGAGTAAT GCGTTGAGAC TGGAGTTCCA TTCGGACAAG 
TCCATTCAGA GATCAGGCTT CGCGGCCGTC TTCTTCACGG ACATCGACGA GTGTGCCGTG 
AATAATGGTG GTTGTCAGCA TGAGTGCCGG AACACGATTG GATCCTACAT CTGCATGTGC 
CACAACGGGT ACTCCATGCA CGAGAATGGC CACGACTGCA AGGAGGGCGA GTGCAAGTAC 
GAGATCTCCG CCCCCTTCGG CACGATTTTC AGTCCCAATT ACCCGGATTC CTATCCCCCA 
AATGCGGACT GCGTTTGGCA CTTCATAACC ACGCCGGGGC ACCGCATCAA GCTGATTTTC 
AACGAGTTCG ACGTGGAATC GCATCAGGAA TGCACCTACG ACAACGTGGC AGTTTACGAT 
GGCGAATCTG AGTCCAGTTC GGTCCTGGGT CGCTTCTGCG GGGATAAGAT TCCCTTCCCC 
ATCTCCTCCA CCAGCAACCA GATGTACATG GTGCTCAAGA CGGACAAGAA CAAGCAAAAG 
AACGGCTTCA CAGCCTCCCA CTCCACGGCC TGTGGGGGCT ACTTGCGTGC CACTAGCCAG 
GTGCAGCAGT TCTATTCGCA CGCCCGATTC GGAAACCAGG ACTATGACGA CGGCATGGAC 
TGCGAGTGGA CCATCGCGGC GCCGGACAAC AGCTACGTCC AGCTCATATT CCTCACCTTC 
GACATCGAGA GCAGCGAGAA CTGCACCTTT GACTACGTGC AGGTGTTCTC TGACATAGAC 
GATGTGTACG GACAGTATGG ACCCATGTAC GGACAGTACT GTGGCAATGT GCTGCCGCAG 
GACATCAACT CTATGACACA TTCTCTGCTG GTTCGCTTTA AGACCGACGG TTCGGTGCCG 
ATGAAGGGCT TCTCTGCCTC ATATGTGGCC GTGCCCAATT CCGGGGAGTA TGACCACTCC 
GACGAGGATG TGGAGAACTC GTACAGCTCC GAGATGGTTA CCCCGTTTCC GGGCTCACTG 
AAGAGCATCT ACATCGAGGA CACGCAGGAG GAGACCGACG AGTACAGCGA CTTCAGCGGC 
AATCAACTGG TCTTCAATGG GCAGTATCGT GGCCGATATA GTCTGCCCAA ACGGTACTAC 
TCCGGAAAGG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287510.1
CDS	325..4719
Translation	MRRRRGTPLGVSWTNCVLLLATGLLVVLISVHAKNPRPHRGLPAPAPAPVPASVDQVATENAHRREPTVDGSVLQRLSGSDRAVDEDEDVDEDEDEGLKHQHLEHRQQFDAYLGTIRRLRRPYRSKYTIEQLMRLHVTPKVSGDIDMDPCKAGGFMGDIALPEGDSGPVVAHTPIDDEEQERQEVEQLLADSPSPVHDPPSVSGAANSGPTKFNATFQLDLEKLKQEVYHEGLQVEEEGLTDIIRKKTKLPSSGPVNLKAGQSQSQSQSANEPVKPGSESPSDYAQASSYKNEVLPPSEQRIHTDKPARLTLAEIGPKFSNKTQSLHRNVDTRKIQSDNKDIEDDSGNNFVAERRPSQIVPTSLPEQRSTPAHVYSNAGGNGMQLDDEVDFLHSRGVVRRRHRRGPKQRRSNQHLEHEVKLQRLREELSRPVIEAHHHQSHHQKQAQHRQHHQSHHRKKHRRQQQHHRRRGQSTTQYVNHSPPGFNQSQQAQQQSVPEMDLLKAETSKDEEPLRHRVARAVTAKKERIWDYGVIPYEIDGNFSGIHKALFKLAMRHWENSTCIKFVERDPEIHPNYIVFTVRSCGCCSFVGKRGNGPQAISIGRNCDKFGIVVHELGHVVGFWHEHTRPDREKHVVIEHNNIMKGQDYNFNMLSPDEVDSLGMAYDYDSIMHYARNTFSKGTYLDTILPIEMKGRKRPEIGQRLRLSQGDIAQANLLYKCPKCGRTFQESSGIFASPSHYTAGALSNETEHCEWRITATYGERVELKLENMNIFKSNNCETDYLEIRDGYFEKSPLIGRFCGKVDKEVIRTESSRMLLTYVNTHRIEGFRGFKAEFDVVCGGELSVDDAVGRLESPNYPLDYLPNKECVWKITVPESYQVALKFQSFEVENHDSCVYDYVEVRDGPGQDAPLIGVFCGYKPPPNMKSSGNSMYVKFVSDTSVQKAGFSAVFMKEVDECETQNHGCEHECINTLGGYECSCRIGFELHSDKKHCEDACGGVIEYPNGTITSPSFPEMYPLLKECIWEIVAPPKHRISLNFTHFDLEGTAHQQSDCGYDSVTVYSKLGENRLKRIGTFCGSSIPPTATSESNALRLEFHSDKSIQRSGFAAVFFTDIDECAVNNGGCQHECRNTIGSYICMCHNGYSMHENGHDCKEGECKYEISAPFGTIFSPNYPDSYPPNADCVWHFITTPGHRIKLIFNEFDVESHQECTYDNVAVYDGESESSSVLGRFCGDKIPFPISSTSNQMYMVLKTDKNKQKNGFTASHSTACGGYLRATSQVQQFYSHARFGNQDYDDGMDCEWTIAAPDNSYVQLIFLTFDIESSENCTFDYVQVFSDIDDVYGQYGPMYGQYCGNVLPQDINSMTHSLLVRFKTDGSVPMKGFSASYVAVPNSGEYDHSDEDVENSYSSEMVTPFPGSLKSIYIEDTQEETDEYSDFSGNQLVFNGQYRGRYSLPKRYYSGKA

Target ORF information:

RefSeq Version	NM_001300581.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tolkin (tok), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300581.1

ORF Insert Sequence:

ATGCGTAGGA GGCGTGGCAC GCCCCTGGGC GTCAGCTGGA CGAACTGCGT CCTGCTCCTG 
GCCACCGGTC TGCTGGTGGT GCTCATTTCG GTGCACGCCA AGAACCCCAG GCCGCATCGA 
GGACTACCCG CCCCGGCTCC TGCACCAGTT CCTGCATCCG TAGATCAGGT GGCAACTGAA 
AATGCCCACA GGCGGGAACC TACCGTCGAT GGGAGTGTAC TTCAACGGCT ATCGGGTTCA 
GATAGGGCTG TTGACGAGGA TGAGGATGTC GATGAGGATG AGGATGAGGG GCTAAAGCAC 
CAGCACCTCG AGCATCGGCA ACAATTTGAC GCATATCTGG GCACAATCCG ACGGCTGCGT 
CGTCCATACC GCAGCAAATA CACCATCGAG CAGCTGATGC GACTGCATGT GACACCAAAG 
GTATCCGGAG ACATTGACAT GGATCCCTGC AAGGCGGGCG GTTTCATGGG CGACATTGCC 
CTGCCGGAGG GTGATTCCGG CCCGGTGGTT GCCCACACGC CCATCGACGA CGAGGAGCAG 
GAGCGCCAGG AGGTGGAGCA GCTGCTGGCT GACTCGCCCA GCCCGGTTCA CGACCCCCCT 
TCCGTTTCCG GTGCGGCCAA CTCAGGGCCG ACCAAGTTCA ATGCCACCTT CCAGCTGGAT 
CTGGAGAAGC TCAAGCAGGA GGTCTACCAC GAGGGCCTAC AGGTGGAGGA GGAGGGCCTC 
ACCGACATCA TCCGCAAGAA GACCAAGCTG CCCAGCTCAG GTCCGGTCAA CCTGAAGGCA 
GGACAAAGCC AGTCACAGAG TCAGAGTGCC AATGAACCCG TGAAGCCCGG TTCGGAATCG 
CCCTCCGATT ACGCGCAAGC ATCCAGCTAC AAGAATGAGG TACTGCCTCC GTCGGAGCAG 
CGAATCCATA CGGACAAGCC GGCACGGTTG ACGCTGGCCG AGATTGGACC CAAGTTCAGC 
AACAAGACGC AGTCGCTGCA CCGTAATGTG GACACACGCA AGATTCAGTC GGACAACAAG 
GACATCGAGG ACGATTCGGG CAACAATTTT GTGGCCGAGA GGAGGCCGTC ACAGATTGTG 
CCCACTTCCT TGCCGGAGCA GCGATCCACT CCGGCTCACG TGTACAGCAA TGCCGGAGGC 
AATGGCATGC AGTTGGACGA CGAGGTGGAC TTCTTGCATT CCCGTGGAGT CGTGAGGAGG 
CGTCACAGGC GAGGACCAAA ACAGCGAAGG AGCAACCAGC ACTTGGAGCA CGAGGTCAAG 
CTGCAGAGAT TGAGGGAGGA GCTCAGCCGT CCGGTCATCG AGGCCCACCA TCACCAGTCG 
CACCACCAGA AGCAGGCGCA GCACCGCCAG CACCACCAGA GCCACCATCG AAAGAAGCAC 
CGTCGACAGC AGCAGCACCA TCGTCGACGT GGCCAGAGCA CCACGCAATA TGTGAACCAT 
TCCCCGCCAG GATTCAATCA ATCGCAGCAG GCCCAACAGC AGTCAGTGCC TGAAATGGAT 
TTGTTGAAAG CGGAAACGTC CAAGGATGAG GAGCCACTGC GTCATCGGGT GGCCCGTGCT 
GTCACCGCCA AAAAGGAACG CATCTGGGAC TATGGGGTGA TTCCGTACGA GATCGATGGC 
AACTTCAGTG GCATCCATAA GGCGCTTTTC AAACTGGCCA TGCGCCATTG GGAGAACTCC 
ACGTGTATCA AGTTCGTAGA GCGCGACCCG GAGATCCATC CCAATTACAT AGTGTTCACG 
GTTCGCAGCT GCGGATGCTG CTCATTTGTG GGCAAACGTG GAAATGGACC GCAGGCCATC 
TCAATTGGTA GAAATTGCGA TAAATTTGGC ATTGTGGTCC ATGAGTTGGG TCACGTGGTG 
GGCTTCTGGC ACGAGCACAC GCGTCCGGAT CGGGAAAAGC ACGTGGTGAT CGAGCATAAC 
AACATTATGA AGGGCCAGGA CTATAACTTC AATATGCTAT CCCCCGATGA AGTGGACTCG 
CTGGGCATGG CCTACGACTA TGATTCGATT ATGCACTACG CACGGAACAC CTTCTCCAAG 
GGCACCTACC TGGACACCAT CCTGCCCATC GAGATGAAGG GTCGCAAGCG TCCTGAAATA 
GGCCAGCGGT TGCGCCTCAG CCAGGGTGAC ATTGCGCAGG CGAATCTTCT GTACAAGTGT 
CCAAAGTGCG GAAGAACCTT TCAGGAGAGT TCCGGCATCT TTGCCTCACC ATCTCATTAC 
ACGGCCGGAG CTTTGAGCAA TGAGACGGAG CACTGCGAGT GGCGGATTAC AGCGACATAC 
GGAGAAAGAG TGGAACTGAA ACTGGAGAAT ATGAACATCT TTAAGTCAAA CAACTGCGAG 
ACGGACTACC TAGAGATTCG GGATGGCTAC TTCGAGAAGT CACCGCTTAT CGGACGCTTC 
TGCGGCAAGG TGGACAAGGA GGTGATCCGC ACGGAGTCCA GTCGCATGCT GCTCACCTAT 
GTGAATACAC ACCGCATCGA GGGCTTCAGG GGCTTCAAGG CGGAGTTCGA TGTTGTTTGC 
GGTGGCGAGT TGTCCGTGGA CGATGCCGTT GGCCGTTTGG AGTCGCCCAA CTATCCACTG 
GACTATTTGC CCAACAAGGA ATGTGTGTGG AAAATAACTG TGCCCGAAAG CTACCAGGTG 
GCGTTGAAAT TCCAGTCCTT CGAAGTGGAG AACCACGACA GCTGTGTCTA CGACTACGTC 
GAGGTCCGGG ACGGTCCTGG CCAGGATGCG CCGCTAATTG GCGTTTTCTG TGGCTACAAG 
CCGCCGCCGA ACATGAAGTC AAGTGGCAAT TCGATGTACG TGAAATTCGT GTCGGACACA 
TCGGTGCAGA AGGCGGGCTT CTCGGCCGTG TTCATGAAGG AGGTGGACGA GTGCGAGACC 
CAGAACCACG GCTGCGAGCA CGAATGCATC AACACCCTCG GTGGCTACGA GTGCAGCTGC 
CGCATCGGAT TCGAGCTCCA CAGCGACAAG AAGCACTGCG AAGATGCCTG TGGCGGTGTC 
ATCGAGTATC CGAATGGCAC CATTACCTCG CCCTCGTTCC CCGAGATGTA CCCTCTGCTG 
AAGGAGTGCA TCTGGGAGAT TGTGGCGCCA CCGAAGCACA GGATATCGCT AAACTTCACC 
CACTTCGACC TTGAGGGCAC GGCGCATCAG CAATCGGACT GTGGCTACGA TTCGGTCACA 
GTCTACTCAA AACTGGGCGA GAATCGTCTG AAGAGGATCG GCACCTTCTG CGGCTCCAGC 
ATTCCGCCTA CGGCCACCTC CGAGAGTAAT GCGTTGAGAC TGGAGTTCCA TTCGGACAAG 
TCCATTCAGA GATCAGGCTT CGCGGCCGTC TTCTTCACGG ACATCGACGA GTGTGCCGTG 
AATAATGGTG GTTGTCAGCA TGAGTGCCGG AACACGATTG GATCCTACAT CTGCATGTGC 
CACAACGGGT ACTCCATGCA CGAGAATGGC CACGACTGCA AGGAGGGCGA GTGCAAGTAC 
GAGATCTCCG CCCCCTTCGG CACGATTTTC AGTCCCAATT ACCCGGATTC CTATCCCCCA 
AATGCGGACT GCGTTTGGCA CTTCATAACC ACGCCGGGGC ACCGCATCAA GCTGATTTTC 
AACGAGTTCG ACGTGGAATC GCATCAGGAA TGCACCTACG ACAACGTGGC AGTTTACGAT 
GGCGAATCTG AGTCCAGTTC GGTCCTGGGT CGCTTCTGCG GGGATAAGAT TCCCTTCCCC 
ATCTCCTCCA CCAGCAACCA GATGTACATG GTGCTCAAGA CGGACAAGAA CAAGCAAAAG 
AACGGCTTCA CAGCCTCCCA CTCCACGGCC TGTGGGGGCT ACTTGCGTGC CACTAGCCAG 
GTGCAGCAGT TCTATTCGCA CGCCCGATTC GGAAACCAGG ACTATGACGA CGGCATGGAC 
TGCGAGTGGA CCATCGCGGC GCCGGACAAC AGCTACGTCC AGCTCATATT CCTCACCTTC 
GACATCGAGA GCAGCGAGAA CTGCACCTTT GACTACGTGC AGGTGTTCTC TGACATAGAC 
GATGTGTACG GACAGTATGG ACCCATGTAC GGACAGTACT GTGGCAATGT GCTGCCGCAG 
GACATCAACT CTATGACACA TTCTCTGCTG GTTCGCTTTA AGACCGACGG TTCGGTGCCG 
ATGAAGGGCT TCTCTGCCTC ATATGTGGCC GTGCCCAATT CCGGGGAGTA TGACCACTCC 
GACGAGGATG TGGAGAACTC GTACAGCTCC GAGATGGTTA CCCCGTTTCC GGGCTCACTG 
AAGAGCATCT ACATCGAGGA CACGCAGGAG GAGACCGACG AGTACAGCGA CTTCAGCGGC 
AATCAACTGG TCTTCAATGG GCAGTATCGT GGCCGATATA GTCTGCCCAA ACGGTACTAC 
TCCGGAAAGG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tok ( NM_170168.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733035.1 Drosophila melanogaster tolkin (tok), transcript variant B, mRNA.

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CloneID	OFa06006
Clone ID Related Accession (Same CDS sequence)	NM_057531.4 , NM_001300581.1 , NM_170168.2
Accession Version	NM_170168.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tolkin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170168.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTAGGA GGCGTGGCAC GCCCCTGGGC GTCAGCTGGA CGAACTGCGT CCTGCTCCTG 
GCCACCGGTC TGCTGGTGGT GCTCATTTCG GTGCACGCCA AGAACCCCAG GCCGCATCGA 
GGACTACCCG CCCCGGCTCC TGCACCAGTT CCTGCATCCG TAGATCAGGT GGCAACTGAA 
AATGCCCACA GGCGGGAACC TACCGTCGAT GGGAGTGTAC TTCAACGGCT ATCGGGTTCA 
GATAGGGCTG TTGACGAGGA TGAGGATGTC GATGAGGATG AGGATGAGGG GCTAAAGCAC 
CAGCACCTCG AGCATCGGCA ACAATTTGAC GCATATCTGG GCACAATCCG ACGGCTGCGT 
CGTCCATACC GCAGCAAATA CACCATCGAG CAGCTGATGC GACTGCATGT GACACCAAAG 
GTATCCGGAG ACATTGACAT GGATCCCTGC AAGGCGGGCG GTTTCATGGG CGACATTGCC 
CTGCCGGAGG GTGATTCCGG CCCGGTGGTT GCCCACACGC CCATCGACGA CGAGGAGCAG 
GAGCGCCAGG AGGTGGAGCA GCTGCTGGCT GACTCGCCCA GCCCGGTTCA CGACCCCCCT 
TCCGTTTCCG GTGCGGCCAA CTCAGGGCCG ACCAAGTTCA ATGCCACCTT CCAGCTGGAT 
CTGGAGAAGC TCAAGCAGGA GGTCTACCAC GAGGGCCTAC AGGTGGAGGA GGAGGGCCTC 
ACCGACATCA TCCGCAAGAA GACCAAGCTG CCCAGCTCAG GTCCGGTCAA CCTGAAGGCA 
GGACAAAGCC AGTCACAGAG TCAGAGTGCC AATGAACCCG TGAAGCCCGG TTCGGAATCG 
CCCTCCGATT ACGCGCAAGC ATCCAGCTAC AAGAATGAGG TACTGCCTCC GTCGGAGCAG 
CGAATCCATA CGGACAAGCC GGCACGGTTG ACGCTGGCCG AGATTGGACC CAAGTTCAGC 
AACAAGACGC AGTCGCTGCA CCGTAATGTG GACACACGCA AGATTCAGTC GGACAACAAG 
GACATCGAGG ACGATTCGGG CAACAATTTT GTGGCCGAGA GGAGGCCGTC ACAGATTGTG 
CCCACTTCCT TGCCGGAGCA GCGATCCACT CCGGCTCACG TGTACAGCAA TGCCGGAGGC 
AATGGCATGC AGTTGGACGA CGAGGTGGAC TTCTTGCATT CCCGTGGAGT CGTGAGGAGG 
CGTCACAGGC GAGGACCAAA ACAGCGAAGG AGCAACCAGC ACTTGGAGCA CGAGGTCAAG 
CTGCAGAGAT TGAGGGAGGA GCTCAGCCGT CCGGTCATCG AGGCCCACCA TCACCAGTCG 
CACCACCAGA AGCAGGCGCA GCACCGCCAG CACCACCAGA GCCACCATCG AAAGAAGCAC 
CGTCGACAGC AGCAGCACCA TCGTCGACGT GGCCAGAGCA CCACGCAATA TGTGAACCAT 
TCCCCGCCAG GATTCAATCA ATCGCAGCAG GCCCAACAGC AGTCAGTGCC TGAAATGGAT 
TTGTTGAAAG CGGAAACGTC CAAGGATGAG GAGCCACTGC GTCATCGGGT GGCCCGTGCT 
GTCACCGCCA AAAAGGAACG CATCTGGGAC TATGGGGTGA TTCCGTACGA GATCGATGGC 
AACTTCAGTG GCATCCATAA GGCGCTTTTC AAACTGGCCA TGCGCCATTG GGAGAACTCC 
ACGTGTATCA AGTTCGTAGA GCGCGACCCG GAGATCCATC CCAATTACAT AGTGTTCACG 
GTTCGCAGCT GCGGATGCTG CTCATTTGTG GGCAAACGTG GAAATGGACC GCAGGCCATC 
TCAATTGGTA GAAATTGCGA TAAATTTGGC ATTGTGGTCC ATGAGTTGGG TCACGTGGTG 
GGCTTCTGGC ACGAGCACAC GCGTCCGGAT CGGGAAAAGC ACGTGGTGAT CGAGCATAAC 
AACATTATGA AGGGCCAGGA CTATAACTTC AATATGCTAT CCCCCGATGA AGTGGACTCG 
CTGGGCATGG CCTACGACTA TGATTCGATT ATGCACTACG CACGGAACAC CTTCTCCAAG 
GGCACCTACC TGGACACCAT CCTGCCCATC GAGATGAAGG GTCGCAAGCG TCCTGAAATA 
GGCCAGCGGT TGCGCCTCAG CCAGGGTGAC ATTGCGCAGG CGAATCTTCT GTACAAGTGT 
CCAAAGTGCG GAAGAACCTT TCAGGAGAGT TCCGGCATCT TTGCCTCACC ATCTCATTAC 
ACGGCCGGAG CTTTGAGCAA TGAGACGGAG CACTGCGAGT GGCGGATTAC AGCGACATAC 
GGAGAAAGAG TGGAACTGAA ACTGGAGAAT ATGAACATCT TTAAGTCAAA CAACTGCGAG 
ACGGACTACC TAGAGATTCG GGATGGCTAC TTCGAGAAGT CACCGCTTAT CGGACGCTTC 
TGCGGCAAGG TGGACAAGGA GGTGATCCGC ACGGAGTCCA GTCGCATGCT GCTCACCTAT 
GTGAATACAC ACCGCATCGA GGGCTTCAGG GGCTTCAAGG CGGAGTTCGA TGTTGTTTGC 
GGTGGCGAGT TGTCCGTGGA CGATGCCGTT GGCCGTTTGG AGTCGCCCAA CTATCCACTG 
GACTATTTGC CCAACAAGGA ATGTGTGTGG AAAATAACTG TGCCCGAAAG CTACCAGGTG 
GCGTTGAAAT TCCAGTCCTT CGAAGTGGAG AACCACGACA GCTGTGTCTA CGACTACGTC 
GAGGTCCGGG ACGGTCCTGG CCAGGATGCG CCGCTAATTG GCGTTTTCTG TGGCTACAAG 
CCGCCGCCGA ACATGAAGTC AAGTGGCAAT TCGATGTACG TGAAATTCGT GTCGGACACA 
TCGGTGCAGA AGGCGGGCTT CTCGGCCGTG TTCATGAAGG AGGTGGACGA GTGCGAGACC 
CAGAACCACG GCTGCGAGCA CGAATGCATC AACACCCTCG GTGGCTACGA GTGCAGCTGC 
CGCATCGGAT TCGAGCTCCA CAGCGACAAG AAGCACTGCG AAGATGCCTG TGGCGGTGTC 
ATCGAGTATC CGAATGGCAC CATTACCTCG CCCTCGTTCC CCGAGATGTA CCCTCTGCTG 
AAGGAGTGCA TCTGGGAGAT TGTGGCGCCA CCGAAGCACA GGATATCGCT AAACTTCACC 
CACTTCGACC TTGAGGGCAC GGCGCATCAG CAATCGGACT GTGGCTACGA TTCGGTCACA 
GTCTACTCAA AACTGGGCGA GAATCGTCTG AAGAGGATCG GCACCTTCTG CGGCTCCAGC 
ATTCCGCCTA CGGCCACCTC CGAGAGTAAT GCGTTGAGAC TGGAGTTCCA TTCGGACAAG 
TCCATTCAGA GATCAGGCTT CGCGGCCGTC TTCTTCACGG ACATCGACGA GTGTGCCGTG 
AATAATGGTG GTTGTCAGCA TGAGTGCCGG AACACGATTG GATCCTACAT CTGCATGTGC 
CACAACGGGT ACTCCATGCA CGAGAATGGC CACGACTGCA AGGAGGGCGA GTGCAAGTAC 
GAGATCTCCG CCCCCTTCGG CACGATTTTC AGTCCCAATT ACCCGGATTC CTATCCCCCA 
AATGCGGACT GCGTTTGGCA CTTCATAACC ACGCCGGGGC ACCGCATCAA GCTGATTTTC 
AACGAGTTCG ACGTGGAATC GCATCAGGAA TGCACCTACG ACAACGTGGC AGTTTACGAT 
GGCGAATCTG AGTCCAGTTC GGTCCTGGGT CGCTTCTGCG GGGATAAGAT TCCCTTCCCC 
ATCTCCTCCA CCAGCAACCA GATGTACATG GTGCTCAAGA CGGACAAGAA CAAGCAAAAG 
AACGGCTTCA CAGCCTCCCA CTCCACGGCC TGTGGGGGCT ACTTGCGTGC CACTAGCCAG 
GTGCAGCAGT TCTATTCGCA CGCCCGATTC GGAAACCAGG ACTATGACGA CGGCATGGAC 
TGCGAGTGGA CCATCGCGGC GCCGGACAAC AGCTACGTCC AGCTCATATT CCTCACCTTC 
GACATCGAGA GCAGCGAGAA CTGCACCTTT GACTACGTGC AGGTGTTCTC TGACATAGAC 
GATGTGTACG GACAGTATGG ACCCATGTAC GGACAGTACT GTGGCAATGT GCTGCCGCAG 
GACATCAACT CTATGACACA TTCTCTGCTG GTTCGCTTTA AGACCGACGG TTCGGTGCCG 
ATGAAGGGCT TCTCTGCCTC ATATGTGGCC GTGCCCAATT CCGGGGAGTA TGACCACTCC 
GACGAGGATG TGGAGAACTC GTACAGCTCC GAGATGGTTA CCCCGTTTCC GGGCTCACTG 
AAGAGCATCT ACATCGAGGA CACGCAGGAG GAGACCGACG AGTACAGCGA CTTCAGCGGC 
AATCAACTGG TCTTCAATGG GCAGTATCGT GGCCGATATA GTCTGCCCAA ACGGTACTAC 
TCCGGAAAGG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733035.1
CDS	208..4602
Translation	MRRRRGTPLGVSWTNCVLLLATGLLVVLISVHAKNPRPHRGLPAPAPAPVPASVDQVATENAHRREPTVDGSVLQRLSGSDRAVDEDEDVDEDEDEGLKHQHLEHRQQFDAYLGTIRRLRRPYRSKYTIEQLMRLHVTPKVSGDIDMDPCKAGGFMGDIALPEGDSGPVVAHTPIDDEEQERQEVEQLLADSPSPVHDPPSVSGAANSGPTKFNATFQLDLEKLKQEVYHEGLQVEEEGLTDIIRKKTKLPSSGPVNLKAGQSQSQSQSANEPVKPGSESPSDYAQASSYKNEVLPPSEQRIHTDKPARLTLAEIGPKFSNKTQSLHRNVDTRKIQSDNKDIEDDSGNNFVAERRPSQIVPTSLPEQRSTPAHVYSNAGGNGMQLDDEVDFLHSRGVVRRRHRRGPKQRRSNQHLEHEVKLQRLREELSRPVIEAHHHQSHHQKQAQHRQHHQSHHRKKHRRQQQHHRRRGQSTTQYVNHSPPGFNQSQQAQQQSVPEMDLLKAETSKDEEPLRHRVARAVTAKKERIWDYGVIPYEIDGNFSGIHKALFKLAMRHWENSTCIKFVERDPEIHPNYIVFTVRSCGCCSFVGKRGNGPQAISIGRNCDKFGIVVHELGHVVGFWHEHTRPDREKHVVIEHNNIMKGQDYNFNMLSPDEVDSLGMAYDYDSIMHYARNTFSKGTYLDTILPIEMKGRKRPEIGQRLRLSQGDIAQANLLYKCPKCGRTFQESSGIFASPSHYTAGALSNETEHCEWRITATYGERVELKLENMNIFKSNNCETDYLEIRDGYFEKSPLIGRFCGKVDKEVIRTESSRMLLTYVNTHRIEGFRGFKAEFDVVCGGELSVDDAVGRLESPNYPLDYLPNKECVWKITVPESYQVALKFQSFEVENHDSCVYDYVEVRDGPGQDAPLIGVFCGYKPPPNMKSSGNSMYVKFVSDTSVQKAGFSAVFMKEVDECETQNHGCEHECINTLGGYECSCRIGFELHSDKKHCEDACGGVIEYPNGTITSPSFPEMYPLLKECIWEIVAPPKHRISLNFTHFDLEGTAHQQSDCGYDSVTVYSKLGENRLKRIGTFCGSSIPPTATSESNALRLEFHSDKSIQRSGFAAVFFTDIDECAVNNGGCQHECRNTIGSYICMCHNGYSMHENGHDCKEGECKYEISAPFGTIFSPNYPDSYPPNADCVWHFITTPGHRIKLIFNEFDVESHQECTYDNVAVYDGESESSSVLGRFCGDKIPFPISSTSNQMYMVLKTDKNKQKNGFTASHSTACGGYLRATSQVQQFYSHARFGNQDYDDGMDCEWTIAAPDNSYVQLIFLTFDIESSENCTFDYVQVFSDIDDVYGQYGPMYGQYCGNVLPQDINSMTHSLLVRFKTDGSVPMKGFSASYVAVPNSGEYDHSDEDVENSYSSEMVTPFPGSLKSIYIEDTQEETDEYSDFSGNQLVFNGQYRGRYSLPKRYYSGKA

Target ORF information:

RefSeq Version	NM_170168.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tolkin (tok), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170168.2

ORF Insert Sequence:

ATGCGTAGGA GGCGTGGCAC GCCCCTGGGC GTCAGCTGGA CGAACTGCGT CCTGCTCCTG 
GCCACCGGTC TGCTGGTGGT GCTCATTTCG GTGCACGCCA AGAACCCCAG GCCGCATCGA 
GGACTACCCG CCCCGGCTCC TGCACCAGTT CCTGCATCCG TAGATCAGGT GGCAACTGAA 
AATGCCCACA GGCGGGAACC TACCGTCGAT GGGAGTGTAC TTCAACGGCT ATCGGGTTCA 
GATAGGGCTG TTGACGAGGA TGAGGATGTC GATGAGGATG AGGATGAGGG GCTAAAGCAC 
CAGCACCTCG AGCATCGGCA ACAATTTGAC GCATATCTGG GCACAATCCG ACGGCTGCGT 
CGTCCATACC GCAGCAAATA CACCATCGAG CAGCTGATGC GACTGCATGT GACACCAAAG 
GTATCCGGAG ACATTGACAT GGATCCCTGC AAGGCGGGCG GTTTCATGGG CGACATTGCC 
CTGCCGGAGG GTGATTCCGG CCCGGTGGTT GCCCACACGC CCATCGACGA CGAGGAGCAG 
GAGCGCCAGG AGGTGGAGCA GCTGCTGGCT GACTCGCCCA GCCCGGTTCA CGACCCCCCT 
TCCGTTTCCG GTGCGGCCAA CTCAGGGCCG ACCAAGTTCA ATGCCACCTT CCAGCTGGAT 
CTGGAGAAGC TCAAGCAGGA GGTCTACCAC GAGGGCCTAC AGGTGGAGGA GGAGGGCCTC 
ACCGACATCA TCCGCAAGAA GACCAAGCTG CCCAGCTCAG GTCCGGTCAA CCTGAAGGCA 
GGACAAAGCC AGTCACAGAG TCAGAGTGCC AATGAACCCG TGAAGCCCGG TTCGGAATCG 
CCCTCCGATT ACGCGCAAGC ATCCAGCTAC AAGAATGAGG TACTGCCTCC GTCGGAGCAG 
CGAATCCATA CGGACAAGCC GGCACGGTTG ACGCTGGCCG AGATTGGACC CAAGTTCAGC 
AACAAGACGC AGTCGCTGCA CCGTAATGTG GACACACGCA AGATTCAGTC GGACAACAAG 
GACATCGAGG ACGATTCGGG CAACAATTTT GTGGCCGAGA GGAGGCCGTC ACAGATTGTG 
CCCACTTCCT TGCCGGAGCA GCGATCCACT CCGGCTCACG TGTACAGCAA TGCCGGAGGC 
AATGGCATGC AGTTGGACGA CGAGGTGGAC TTCTTGCATT CCCGTGGAGT CGTGAGGAGG 
CGTCACAGGC GAGGACCAAA ACAGCGAAGG AGCAACCAGC ACTTGGAGCA CGAGGTCAAG 
CTGCAGAGAT TGAGGGAGGA GCTCAGCCGT CCGGTCATCG AGGCCCACCA TCACCAGTCG 
CACCACCAGA AGCAGGCGCA GCACCGCCAG CACCACCAGA GCCACCATCG AAAGAAGCAC 
CGTCGACAGC AGCAGCACCA TCGTCGACGT GGCCAGAGCA CCACGCAATA TGTGAACCAT 
TCCCCGCCAG GATTCAATCA ATCGCAGCAG GCCCAACAGC AGTCAGTGCC TGAAATGGAT 
TTGTTGAAAG CGGAAACGTC CAAGGATGAG GAGCCACTGC GTCATCGGGT GGCCCGTGCT 
GTCACCGCCA AAAAGGAACG CATCTGGGAC TATGGGGTGA TTCCGTACGA GATCGATGGC 
AACTTCAGTG GCATCCATAA GGCGCTTTTC AAACTGGCCA TGCGCCATTG GGAGAACTCC 
ACGTGTATCA AGTTCGTAGA GCGCGACCCG GAGATCCATC CCAATTACAT AGTGTTCACG 
GTTCGCAGCT GCGGATGCTG CTCATTTGTG GGCAAACGTG GAAATGGACC GCAGGCCATC 
TCAATTGGTA GAAATTGCGA TAAATTTGGC ATTGTGGTCC ATGAGTTGGG TCACGTGGTG 
GGCTTCTGGC ACGAGCACAC GCGTCCGGAT CGGGAAAAGC ACGTGGTGAT CGAGCATAAC 
AACATTATGA AGGGCCAGGA CTATAACTTC AATATGCTAT CCCCCGATGA AGTGGACTCG 
CTGGGCATGG CCTACGACTA TGATTCGATT ATGCACTACG CACGGAACAC CTTCTCCAAG 
GGCACCTACC TGGACACCAT CCTGCCCATC GAGATGAAGG GTCGCAAGCG TCCTGAAATA 
GGCCAGCGGT TGCGCCTCAG CCAGGGTGAC ATTGCGCAGG CGAATCTTCT GTACAAGTGT 
CCAAAGTGCG GAAGAACCTT TCAGGAGAGT TCCGGCATCT TTGCCTCACC ATCTCATTAC 
ACGGCCGGAG CTTTGAGCAA TGAGACGGAG CACTGCGAGT GGCGGATTAC AGCGACATAC 
GGAGAAAGAG TGGAACTGAA ACTGGAGAAT ATGAACATCT TTAAGTCAAA CAACTGCGAG 
ACGGACTACC TAGAGATTCG GGATGGCTAC TTCGAGAAGT CACCGCTTAT CGGACGCTTC 
TGCGGCAAGG TGGACAAGGA GGTGATCCGC ACGGAGTCCA GTCGCATGCT GCTCACCTAT 
GTGAATACAC ACCGCATCGA GGGCTTCAGG GGCTTCAAGG CGGAGTTCGA TGTTGTTTGC 
GGTGGCGAGT TGTCCGTGGA CGATGCCGTT GGCCGTTTGG AGTCGCCCAA CTATCCACTG 
GACTATTTGC CCAACAAGGA ATGTGTGTGG AAAATAACTG TGCCCGAAAG CTACCAGGTG 
GCGTTGAAAT TCCAGTCCTT CGAAGTGGAG AACCACGACA GCTGTGTCTA CGACTACGTC 
GAGGTCCGGG ACGGTCCTGG CCAGGATGCG CCGCTAATTG GCGTTTTCTG TGGCTACAAG 
CCGCCGCCGA ACATGAAGTC AAGTGGCAAT TCGATGTACG TGAAATTCGT GTCGGACACA 
TCGGTGCAGA AGGCGGGCTT CTCGGCCGTG TTCATGAAGG AGGTGGACGA GTGCGAGACC 
CAGAACCACG GCTGCGAGCA CGAATGCATC AACACCCTCG GTGGCTACGA GTGCAGCTGC 
CGCATCGGAT TCGAGCTCCA CAGCGACAAG AAGCACTGCG AAGATGCCTG TGGCGGTGTC 
ATCGAGTATC CGAATGGCAC CATTACCTCG CCCTCGTTCC CCGAGATGTA CCCTCTGCTG 
AAGGAGTGCA TCTGGGAGAT TGTGGCGCCA CCGAAGCACA GGATATCGCT AAACTTCACC 
CACTTCGACC TTGAGGGCAC GGCGCATCAG CAATCGGACT GTGGCTACGA TTCGGTCACA 
GTCTACTCAA AACTGGGCGA GAATCGTCTG AAGAGGATCG GCACCTTCTG CGGCTCCAGC 
ATTCCGCCTA CGGCCACCTC CGAGAGTAAT GCGTTGAGAC TGGAGTTCCA TTCGGACAAG 
TCCATTCAGA GATCAGGCTT CGCGGCCGTC TTCTTCACGG ACATCGACGA GTGTGCCGTG 
AATAATGGTG GTTGTCAGCA TGAGTGCCGG AACACGATTG GATCCTACAT CTGCATGTGC 
CACAACGGGT ACTCCATGCA CGAGAATGGC CACGACTGCA AGGAGGGCGA GTGCAAGTAC 
GAGATCTCCG CCCCCTTCGG CACGATTTTC AGTCCCAATT ACCCGGATTC CTATCCCCCA 
AATGCGGACT GCGTTTGGCA CTTCATAACC ACGCCGGGGC ACCGCATCAA GCTGATTTTC 
AACGAGTTCG ACGTGGAATC GCATCAGGAA TGCACCTACG ACAACGTGGC AGTTTACGAT 
GGCGAATCTG AGTCCAGTTC GGTCCTGGGT CGCTTCTGCG GGGATAAGAT TCCCTTCCCC 
ATCTCCTCCA CCAGCAACCA GATGTACATG GTGCTCAAGA CGGACAAGAA CAAGCAAAAG 
AACGGCTTCA CAGCCTCCCA CTCCACGGCC TGTGGGGGCT ACTTGCGTGC CACTAGCCAG 
GTGCAGCAGT TCTATTCGCA CGCCCGATTC GGAAACCAGG ACTATGACGA CGGCATGGAC 
TGCGAGTGGA CCATCGCGGC GCCGGACAAC AGCTACGTCC AGCTCATATT CCTCACCTTC 
GACATCGAGA GCAGCGAGAA CTGCACCTTT GACTACGTGC AGGTGTTCTC TGACATAGAC 
GATGTGTACG GACAGTATGG ACCCATGTAC GGACAGTACT GTGGCAATGT GCTGCCGCAG 
GACATCAACT CTATGACACA TTCTCTGCTG GTTCGCTTTA AGACCGACGG TTCGGTGCCG 
ATGAAGGGCT TCTCTGCCTC ATATGTGGCC GTGCCCAATT CCGGGGAGTA TGACCACTCC 
GACGAGGATG TGGAGAACTC GTACAGCTCC GAGATGGTTA CCCCGTTTCC GGGCTCACTG 
AAGAGCATCT ACATCGAGGA CACGCAGGAG GAGACCGACG AGTACAGCGA CTTCAGCGGC 
AATCAACTGG TCTTCAATGG GCAGTATCGT GGCCGATATA GTCTGCCCAA ACGGTACTAC 
TCCGGAAAGG CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tok cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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