gsap cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	gsap
Entrez Gene ID	100494433
Full Name	gamma-secretase activating protein
General protein information	Preferred Names gamma-secretase activating protein Names gamma-secretase-activating protein protein pigeon homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004913025.3	XP_004913082.1	gamma-secretase-activating protein isoform X2
XM_012960012.2	XP_012815466.1	gamma-secretase-activating protein isoform X1
XM_012960013.2	XP_012815467.1	gamma-secretase-activating protein isoform X3
XM_012960013.3	XP_012815467.2	gamma-secretase-activating protein isoform X3
XM_031899025.1	XP_031754885.1	gamma-secretase-activating protein isoform X4
XM_012960014.2	XP_012815468.1	gamma-secretase-activating protein isoform X4
XM_012960012.3	XP_012815466.2	gamma-secretase-activating protein isoform X1
XM_004913025.4	XP_004913082.2	gamma-secretase-activating protein isoform X2

Pathways from BioSystems
Gene Ontology

The following gsap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gsap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa07903	XM_004913025.3 Latest version!	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa35976	XM_012960012.2 Latest version!	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa35977	XM_012960013.2 Latest version!	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa35978	XM_012960014.2 Latest version!	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa54861	XM_012960012.3 Latest version!	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa54862	XM_004913025.4 Latest version!	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa54863	XM_012960013.3 Latest version!	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa54864	XM_031899025.1 Latest version!	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gsap ( XM_004913025.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913082.1 Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa07903
Clone ID Related Accession (Same CDS sequence)	XM_004913025.3
Accession Version	XM_004913025.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gamma-secretase-activating protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACATC AGAGACTTTT TTACATTATT CCTAAGCATT CCAGTTGCGT CTTGCATTGC 
ATCCAGTTTT ATCATGAAGA TCATTTCAAA ATAATTTTTG AAGTTTCTCT TGAGATAATT 
TTCGTTTACC AAGCAGTATG TTTGATCAAT TTGGGCTTTG ACCCTTATGA AGTGAAAGAG 
CAAGAAAGTT CTACATCTCT AAACATACAG GTGTTTACAG ATAATACAGG AGGTCTGTGC 
TTGTTTTATC TCCAGCCTTT CAAAGATGCA AAGGAGGTGA AATACATGGT GATATTTTTG 
CACAGAGGTT GTAGTAAGAC CTTTAAAGTT GCATTATCCG CAGAAGAGAT GAAACAGCTA 
AAGAAAATGT CTTTTATTAA TCTTGGTGGT TATGTTGCTG TTTACTTGCC TGACCATTTT 
TTGCATTTGA TAAACACCAG GCATCCTGAC TTGATGTGCT ACCACTTGTT TTTGGCAGAT 
ACTGATGCCA GAATAGGTGA GATATGCTGT GATGGTCCTG TTCAGTCTGT TCTAATGTCG 
TCAGTGATTG AGTACTGCCA GGGGATTCTC TTCTCAGTCA GCCTAAACCA CTTGCTTCTT 
CTGAAATACC TTTGGACTTG TAAAAGGGAT TGTGAAAGGC TGGCAGTACT TCATTGTTTT 
CTTTTGCACC TGGATCAGAA GCCACTATGG GAAACAGAGA TTATTGAGTG GATATGTGAA 
AACCTCTCAG TATGCCAAAT GTTTGATCCC ATGCAGGAAT TTATAATTGC TTCTTTATAC 
AGAAGAATGA GTTTGGAAAC TATTTATCTA GATAAACTGC TGCCTTATAC TTCACTGCCT 
TTTTGGAATC AGGACATTGA GGGTGTGTTA TGGTCATCAG ACATCACAGA TATGCCAATA 
TTAAAGATTG GTATGTTTAA AGGATTCTGG GAGAAGTTCC ATTCTGAGCT GGAATATATG 
AAACATACTC ACCACAATAA CCCTGTGCAC CGAAGAGATT GGTGTAAACA AATTGCTGAT 
GTGGACACAC AAGAATGGAG GAATATTATT TATCAGCGCA ACATTTTGGA AAATGCAAAG 
AAAGTTATTT TAAACATGGA AACATGGAGT TCTGATCAGA GAATTGTGCC GCTTCATCAG 
GAAGAGGATT ATCTACAGAA AGACCTCATG TGGCTGATGA CGATTAAATT GAAGGATCAC 
CTGTCACAGC CTTTGCTTCA TGTTGGAAAA AATAACATTG ATAAGATTGT TCTGGATTAC 
ATTTCAAAGC AGTTAGACTT GATATGCCTG ATACTGGAAG TTGTGTGGAA AAAATACAAC 
TTGGATCTAT ATGCATTCTC TTTTAGTTCA AGAGGATGTT CTTCAGACTA TTCTGCCTTT 
TACATGATGT GCCACATTTC AGAATCTGCA ATCAAACTGG GCATGCCTCT TCCACCTGGA 
TTCCAGACAC TACATTTGGT CCTGGGAGTT CGATGCCTTC CTCTAGGAAA TCTTCTGCAT 
TATATAGATG CTGGAATTCT ACAACTGACT GAAGCTTTTG CTGTCAAACT TCTAAAAGAA 
CTGGATGACA ATGAAAGCAA TGAAAAGTTA AAATACAGCA TTATTGTACG TCTTCCTGAG 
AGAATTTGCC AAAATATTCA TCGCTTATGG GATCATGCCA TTAGTAATAA CTGCATCGCC 
AGAAAATATG TTGAACAATT ATTGTGCAAG CTTAAAAAAA GGGAGTGTCA CCTTGCAGGA 
CAAGCAGCTT GTAGTCAGTC AGCATTGTCC ATGAATTTCT TGCCACTTAA CTACTTAATC 
AAGATGCTGT CAACAATAGA AGAACAAGCA TTAAATCCCT TTGAAGAAGA TAACATAGAT 
GCTGCATTTC TGGAGGAAAT CGCCTTAAAA CAGACTACTG TCCTTCTTAG GTTACACAAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913082.1
CDS	4..1929
Translation	MLHQRLFYIIPKHSSCVLHCIQFYHEDHFKIIFEVSLEIIFVYQAVCLINLGFDPYEVKEQESSTSLNIQVFTDNTGGLCLFYLQPFKDAKEVKYMVIFLHRGCSKTFKVALSAEEMKQLKKMSFINLGGYVAVYLPDHFLHLINTRHPDLMCYHLFLADTDARIGEICCDGPVQSVLMSSVIEYCQGILFSVSLNHLLLLKYLWTCKRDCERLAVLHCFLLHLDQKPLWETEIIEWICENLSVCQMFDPMQEFIIASLYRRMSLETIYLDKLLPYTSLPFWNQDIEGVLWSSDITDMPILKIGMFKGFWEKFHSELEYMKHTHHNNPVHRRDWCKQIADVDTQEWRNIIYQRNILENAKKVILNMETWSSDQRIVPLHQEEDYLQKDLMWLMTIKLKDHLSQPLLHVGKNNIDKIVLDYISKQLDLICLILEVVWKKYNLDLYAFSFSSRGCSSDYSAFYMMCHISESAIKLGMPLPPGFQTLHLVLGVRCLPLGNLLHYIDAGILQLTEAFAVKLLKELDDNESNEKLKYSIIVRLPERICQNIHRLWDHAISNNCIARKYVEQLLCKLKKRECHLAGQAACSQSALSMNFLPLNYLIKMLSTIEEQALNPFEEDNIDAAFLEEIALKQTTVLLRLHKS

Target ORF information:

RefSeq Version	XM_004913025.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913025.3

ORF Insert Sequence:

ATGTTACATC AGAGACTTTT TTACATTATT CCTAAGCATT CCAGTTGCGT CTTGCATTGC 
ATCCAGTTTT ATCATGAAGA TCATTTCAAA ATAATTTTTG AAGTTTCTCT TGAGATAATT 
TTCGTTTACC AAGCAGTATG TTTGATCAAT TTGGGCTTTG ACCCTTATGA AGTGAAAGAG 
CAAGAAAGTT CTACATCTCT AAACATACAG GTGTTTACAG ATAATACAGG AGGTCTGTGC 
TTGTTTTATC TCCAGCCTTT CAAAGATGCA AAGGAGGTGA AATACATGGT GATATTTTTG 
CACAGAGGTT GTAGTAAGAC CTTTAAAGTT GCATTATCCG CAGAAGAGAT GAAACAGCTA 
AAGAAAATGT CTTTTATTAA TCTTGGTGGT TATGTTGCTG TTTACTTGCC TGACCATTTT 
TTGCATTTGA TAAACACCAG GCATCCTGAC TTGATGTGCT ACCACTTGTT TTTGGCAGAT 
ACTGATGCCA GAATAGGTGA GATATGCTGT GATGGTCCTG TTCAGTCTGT TCTAATGTCG 
TCAGTGATTG AGTACTGCCA GGGGATTCTC TTCTCAGTCA GCCTAAACCA CTTGCTTCTT 
CTGAAATACC TTTGGACTTG TAAAAGGGAT TGTGAAAGGC TGGCAGTACT TCATTGTTTT 
CTTTTGCACC TGGATCAGAA GCCACTATGG GAAACAGAGA TTATTGAGTG GATATGTGAA 
AACCTCTCAG TATGCCAAAT GTTTGATCCC ATGCAGGAAT TTATAATTGC TTCTTTATAC 
AGAAGAATGA GTTTGGAAAC TATTTATCTA GATAAACTGC TGCCTTATAC TTCACTGCCT 
TTTTGGAATC AGGACATTGA GGGTGTGTTA TGGTCATCAG ACATCACAGA TATGCCAATA 
TTAAAGATTG GTATGTTTAA AGGATTCTGG GAGAAGTTCC ATTCTGAGCT GGAATATATG 
AAACATACTC ACCACAATAA CCCTGTGCAC CGAAGAGATT GGTGTAAACA AATTGCTGAT 
GTGGACACAC AAGAATGGAG GAATATTATT TATCAGCGCA ACATTTTGGA AAATGCAAAG 
AAAGTTATTT TAAACATGGA AACATGGAGT TCTGATCAGA GAATTGTGCC GCTTCATCAG 
GAAGAGGATT ATCTACAGAA AGACCTCATG TGGCTGATGA CGATTAAATT GAAGGATCAC 
CTGTCACAGC CTTTGCTTCA TGTTGGAAAA AATAACATTG ATAAGATTGT TCTGGATTAC 
ATTTCAAAGC AGTTAGACTT GATATGCCTG ATACTGGAAG TTGTGTGGAA AAAATACAAC 
TTGGATCTAT ATGCATTCTC TTTTAGTTCA AGAGGATGTT CTTCAGACTA TTCTGCCTTT 
TACATGATGT GCCACATTTC AGAATCTGCA ATCAAACTGG GCATGCCTCT TCCACCTGGA 
TTCCAGACAC TACATTTGGT CCTGGGAGTT CGATGCCTTC CTCTAGGAAA TCTTCTGCAT 
TATATAGATG CTGGAATTCT ACAACTGACT GAAGCTTTTG CTGTCAAACT TCTAAAAGAA 
CTGGATGACA ATGAAAGCAA TGAAAAGTTA AAATACAGCA TTATTGTACG TCTTCCTGAG 
AGAATTTGCC AAAATATTCA TCGCTTATGG GATCATGCCA TTAGTAATAA CTGCATCGCC 
AGAAAATATG TTGAACAATT ATTGTGCAAG CTTAAAAAAA GGGAGTGTCA CCTTGCAGGA 
CAAGCAGCTT GTAGTCAGTC AGCATTGTCC ATGAATTTCT TGCCACTTAA CTACTTAATC 
AAGATGCTGT CAACAATAGA AGAACAAGCA TTAAATCCCT TTGAAGAAGA TAACATAGAT 
GCTGCATTTC TGGAGGAAAT CGCCTTAAAA CAGACTACTG TCCTTCTTAG GTTACACAAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsap ( XM_012960012.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815466.1 Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa35976
Clone ID Related Accession (Same CDS sequence)	XM_012960012.2
Accession Version	XM_012960012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gamma-secretase-activating protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACATC AGAGACTTTT TTACATTATT CCTAAGCATT CCAGTTGCGT CTTGCATTGC 
ATCCAGTTTT ATCATGAAGA TCATTTCAAA ATAATTTTTG AAGTTTCTCT TGAGATAATT 
TTCGTTTACC AAGCAGTATG TTTGATCAAT TTGGGCTTTG ACCCTTATGA AGTGAAAGAG 
CAAGAAAGTT CTACATCTCT AAACATACAG GTGTTTACAG ATAATACAGG AGGTCTGTGC 
TTGTTTTATC TCCAGCCTTT CAAAGATGCA AAGGAGGTGA AATACATGGT GATATTTTTG 
CACAGAGGTT GTAGTAAGAC CTTTAAAGTT GCATTATCCG CAGAAGAGAT GAAACAGCTA 
AAGAAAATGT CTTTTATTAA TCTTGGTGGT TATGTTGCTG TTTACTTGCC TGACCATTTT 
TTGCATTTGA TAAACACCAG GCATCCTGAC TTGATGTGCT ACCACTTGTT TTTGGCAGAT 
ACTGATGCCA GAATAGGTGA GATATGCTGT GATGGTCCTG TTCAGTCTGT TCTAATGTCG 
TCAGTGATTG AGTACTGCCA GGGGATTCTC TTCTCAGTCA GCCTAAACCA CTTGCTTCTT 
CTGAAATACC TTTGGACTTG TAAAAGGGAT TGTGAAAGGC TGGCAGTACT TCATTGTTTT 
CTTTTGCACC TGGATCAGAA GCCACTATGG GAAACAGAGA TTATTGAGTG GATATGTGAA 
AACCTCTCAG TATGCCAAAT GTTTGATCCC ATGCAGGAAT TTATAATTGC TTCTTTATAC 
AGAAGAATGA GTTTGGAAAC TATTTATCTA GATAAACTGC TGCCTTATAC TTCACTGCCT 
TTTTGGAATC AGGACATTGA GGGTGTGTTA TGGTCATCAG ACATCACAGA TATGCCAATA 
TTAAAGATTG GTATGTTTAA AGGATTCTGG GAGAAGTTCC ATTCTGAGCT GGAATATATG 
AAACATACTC ACCACAATAA CCCTGTGCAC CGAAGAGATT GGTGTAAACA AATTGCTGAT 
GTGGACACAC AAGAATGGAG GAATATTATT TATCAGCGCA ACATTTTGGA AAATGCAAAG 
AAAGTTATTT TAAACATGGA AACATGGAGT TCTGATCAGA GAATTGTGCC GCTTCATCAG 
GAAGAGGATT ATCTACAGAA AGACCTCATG TGGCTGATGA CGATTAAATT GAAGGATCAC 
CTGTCACAGC CTTTGCTTCA TGTTGGAAAA AATAACATTG ATAAGATTGT TCTGGATTAC 
ATTTCAAAGC AGTTAGACTT GATATGCCTG ATACTGGAAG TTGTGTGGAA AAAATACAAC 
TTGGATCTAT ATGCATTCTC TTTCCTATTT ATTTGCAGTA GTTCAAGAGG ATGTTCTTCA 
GACTATTCTG CCTTTTACAT GATGTGCCAC ATTTCAGAAT CTGCAATCAA ACTGGGCATG 
CCTCTTCCAC CTGGATTCCA GACACTACAT TTGGTCCTGG GAGTTCGATG CCTTCCTCTA 
GGAAATCTTC TGCATTATAT AGATGCTGGA ATTCTACAAC TGACTGAAGC TTTTGCTGTC 
AAACTTCTAA AAGAACTGGA TGACAATGAA AGCAATGAAA AGTTAAAATA CAGCATTATT 
GTACGTCTTC CTGAGAGAAT TTGCCAAAAT ATTCATCGCT TATGGGATCA TGCCATTAGT 
AATAACTGCA TCGCCAGAAA ATATGTTGAA CAATTATTGT GCAAGCTTAA AAAAAGGGAG 
TGTCACCTTG CAGGACAAGC AGCTTGTAGT CAGTCAGCAT TGTCCATGAA TTTCTTGCCA 
CTTAACTACT TAATCAAGAT GCTGTCAACA ATAGAAGAAC AAGCATTAAA TCCCTTTGAA 
GAAGATAACA TAGATGCTGC ATTTCTGGAG GAAATCGCCT TAAAACAGAC TACTGTCCTT 
CTTAGGTTAC ACAAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815466.1
CDS	4..1944
Translation	MLHQRLFYIIPKHSSCVLHCIQFYHEDHFKIIFEVSLEIIFVYQAVCLINLGFDPYEVKEQESSTSLNIQVFTDNTGGLCLFYLQPFKDAKEVKYMVIFLHRGCSKTFKVALSAEEMKQLKKMSFINLGGYVAVYLPDHFLHLINTRHPDLMCYHLFLADTDARIGEICCDGPVQSVLMSSVIEYCQGILFSVSLNHLLLLKYLWTCKRDCERLAVLHCFLLHLDQKPLWETEIIEWICENLSVCQMFDPMQEFIIASLYRRMSLETIYLDKLLPYTSLPFWNQDIEGVLWSSDITDMPILKIGMFKGFWEKFHSELEYMKHTHHNNPVHRRDWCKQIADVDTQEWRNIIYQRNILENAKKVILNMETWSSDQRIVPLHQEEDYLQKDLMWLMTIKLKDHLSQPLLHVGKNNIDKIVLDYISKQLDLICLILEVVWKKYNLDLYAFSFLFICSSSRGCSSDYSAFYMMCHISESAIKLGMPLPPGFQTLHLVLGVRCLPLGNLLHYIDAGILQLTEAFAVKLLKELDDNESNEKLKYSIIVRLPERICQNIHRLWDHAISNNCIARKYVEQLLCKLKKRECHLAGQAACSQSALSMNFLPLNYLIKMLSTIEEQALNPFEEDNIDAAFLEEIALKQTTVLLRLHKS

Target ORF information:

RefSeq Version	XM_012960012.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960012.2

ORF Insert Sequence:

ATGTTACATC AGAGACTTTT TTACATTATT CCTAAGCATT CCAGTTGCGT CTTGCATTGC 
ATCCAGTTTT ATCATGAAGA TCATTTCAAA ATAATTTTTG AAGTTTCTCT TGAGATAATT 
TTCGTTTACC AAGCAGTATG TTTGATCAAT TTGGGCTTTG ACCCTTATGA AGTGAAAGAG 
CAAGAAAGTT CTACATCTCT AAACATACAG GTGTTTACAG ATAATACAGG AGGTCTGTGC 
TTGTTTTATC TCCAGCCTTT CAAAGATGCA AAGGAGGTGA AATACATGGT GATATTTTTG 
CACAGAGGTT GTAGTAAGAC CTTTAAAGTT GCATTATCCG CAGAAGAGAT GAAACAGCTA 
AAGAAAATGT CTTTTATTAA TCTTGGTGGT TATGTTGCTG TTTACTTGCC TGACCATTTT 
TTGCATTTGA TAAACACCAG GCATCCTGAC TTGATGTGCT ACCACTTGTT TTTGGCAGAT 
ACTGATGCCA GAATAGGTGA GATATGCTGT GATGGTCCTG TTCAGTCTGT TCTAATGTCG 
TCAGTGATTG AGTACTGCCA GGGGATTCTC TTCTCAGTCA GCCTAAACCA CTTGCTTCTT 
CTGAAATACC TTTGGACTTG TAAAAGGGAT TGTGAAAGGC TGGCAGTACT TCATTGTTTT 
CTTTTGCACC TGGATCAGAA GCCACTATGG GAAACAGAGA TTATTGAGTG GATATGTGAA 
AACCTCTCAG TATGCCAAAT GTTTGATCCC ATGCAGGAAT TTATAATTGC TTCTTTATAC 
AGAAGAATGA GTTTGGAAAC TATTTATCTA GATAAACTGC TGCCTTATAC TTCACTGCCT 
TTTTGGAATC AGGACATTGA GGGTGTGTTA TGGTCATCAG ACATCACAGA TATGCCAATA 
TTAAAGATTG GTATGTTTAA AGGATTCTGG GAGAAGTTCC ATTCTGAGCT GGAATATATG 
AAACATACTC ACCACAATAA CCCTGTGCAC CGAAGAGATT GGTGTAAACA AATTGCTGAT 
GTGGACACAC AAGAATGGAG GAATATTATT TATCAGCGCA ACATTTTGGA AAATGCAAAG 
AAAGTTATTT TAAACATGGA AACATGGAGT TCTGATCAGA GAATTGTGCC GCTTCATCAG 
GAAGAGGATT ATCTACAGAA AGACCTCATG TGGCTGATGA CGATTAAATT GAAGGATCAC 
CTGTCACAGC CTTTGCTTCA TGTTGGAAAA AATAACATTG ATAAGATTGT TCTGGATTAC 
ATTTCAAAGC AGTTAGACTT GATATGCCTG ATACTGGAAG TTGTGTGGAA AAAATACAAC 
TTGGATCTAT ATGCATTCTC TTTCCTATTT ATTTGCAGTA GTTCAAGAGG ATGTTCTTCA 
GACTATTCTG CCTTTTACAT GATGTGCCAC ATTTCAGAAT CTGCAATCAA ACTGGGCATG 
CCTCTTCCAC CTGGATTCCA GACACTACAT TTGGTCCTGG GAGTTCGATG CCTTCCTCTA 
GGAAATCTTC TGCATTATAT AGATGCTGGA ATTCTACAAC TGACTGAAGC TTTTGCTGTC 
AAACTTCTAA AAGAACTGGA TGACAATGAA AGCAATGAAA AGTTAAAATA CAGCATTATT 
GTACGTCTTC CTGAGAGAAT TTGCCAAAAT ATTCATCGCT TATGGGATCA TGCCATTAGT 
AATAACTGCA TCGCCAGAAA ATATGTTGAA CAATTATTGT GCAAGCTTAA AAAAAGGGAG 
TGTCACCTTG CAGGACAAGC AGCTTGTAGT CAGTCAGCAT TGTCCATGAA TTTCTTGCCA 
CTTAACTACT TAATCAAGAT GCTGTCAACA ATAGAAGAAC AAGCATTAAA TCCCTTTGAA 
GAAGATAACA TAGATGCTGC ATTTCTGGAG GAAATCGCCT TAAAACAGAC TACTGTCCTT 
CTTAGGTTAC ACAAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsap ( XM_012960013.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815467.1 Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa35977
Clone ID Related Accession (Same CDS sequence)	XM_012960013.2
Accession Version	XM_012960013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gamma-secretase-activating protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACATC AGAGACTTTT TTACATTATT CCTAAGCATT CCAGTTGCGT CTTGCATTGC 
ATCCAGTTTT ATCATGAAGA TCATTTCAAA ATAATTTTTG AAGTTTCTCT TGAGATAATT 
TTCGTTTACC AAGCAGTATG TTTGATCAAT TTGGGCTTTG ACCCTTATGA AGTGAAAGAG 
CAAGAAAGTT CTACATCTCT AAACATACAG GTGTTTACAG ATAATACAGG AGGTCTGTGC 
TTGTTTTATC TCCAGCCTTT CAAAGATGCA AAGGAGGTGA AATACATGGT GATATTTTTG 
CACAGAGGTT GTAGTAAGAC CTTTAAAGTT GCATTATCCG CAGAAGAGAT GAAACAGCTA 
AAGAAAATGT CTTTTATTAA TCTTGGTGGT TATGTTGCTG TTTACTTGCC TGACCATTTT 
TTGCATTTGA TAAACACCAG GCATCCTGAC TTGATGTGCT ACCACTTGTT TTTGGCAGAT 
ACTGATGCCA GAATAGGTGA GATATGCTGT GATGGTCCTG TTCAGTCTGT TCTAATGTCG 
TCAGTGATTG AGTACTGCCA GGGGATTCTC TTCTCAGTCA GCCTAAACCA CTTGCTTCTT 
CTGAAATACC TTTGGACTTG TAAAAGGGAT TGTGAAAGGC TGGCAGTACT TCATTGTTTT 
CTTTTGCACC TGGATCAGAA GCCACTATGG GAAACAGAGA TTATTGAGTG GATATGTGAA 
AACCTCTCAG TATGCCAAAT GTTTGATCCC ATGCAGGAAT TTATAATTGC TTCTTTATAC 
AGAAGAATGA GTTTGGAAAC TATTTATCTA GATAAACTGC TGCCTTATAC TTCACTGCCT 
TTTTGGAATC AGGACATTGA GGGTGTGTTA TGGTCATCAG ACATCACAGA TATGCCAATA 
TTAAAGATTG GTATGTTTAA AGGATTCTGG GAGAAGTTCC ATTCTGAGCT GGAATATATG 
AAACATACTC ACCACAATAA CCCTGTGCAC CGAAGAGATT GGTGTAAACA AATTGCTGAT 
GTGGACACAC AAGAATGGAG GAATATTATT TATCAGCGCA ACATTTTGGA AAATGCAAAG 
AAAGTTATTT TAAACATGGA AACATGGAGT TCTGATCAGA GAATTGTGCC GCTTCATCAG 
GAAGAGGATT ATCTACAGAA AGACCTCATG TGGCTGATGA CGATTAAATT GAAGGATCAC 
CTGTCACAGC CTTTGCTTCA TGTTGGAAAA AATAACATTG ATAAGATTGT TCTGGATTAC 
ATTTCAAAGC AGTTAGACTT GATATGCCTG ATACTGGAAG TTGTGTGGAA AAAATACAAC 
TTGGATCTAT ATGCATTCTC TTTTTCAAGA GGATGTTCTT CAGACTATTC TGCCTTTTAC 
ATGATGTGCC ACATTTCAGA ATCTGCAATC AAACTGGGCA TGCCTCTTCC ACCTGGATTC 
CAGACACTAC ATTTGGTCCT GGGAGTTCGA TGCCTTCCTC TAGGAAATCT TCTGCATTAT 
ATAGATGCTG GAATTCTACA ACTGACTGAA GCTTTTGCTG TCAAACTTCT AAAAGAACTG 
GATGACAATG AAAGCAATGA AAAGTTAAAA TACAGCATTA TTGTACGTCT TCCTGAGAGA 
ATTTGCCAAA ATATTCATCG CTTATGGGAT CATGCCATTA GTAATAACTG CATCGCCAGA 
AAATATGTTG AACAATTATT GTGCAAGCTT AAAAAAAGGG AGTGTCACCT TGCAGGACAA 
GCAGCTTGTA GTCAGTCAGC ATTGTCCATG AATTTCTTGC CACTTAACTA CTTAATCAAG 
ATGCTGTCAA CAATAGAAGA ACAAGCATTA AATCCCTTTG AAGAAGATAA CATAGATGCT 
GCATTTCTGG AGGAAATCGC CTTAAAACAG ACTACTGTCC TTCTTAGGTT ACACAAAAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815467.1
CDS	4..1926
Translation	MLHQRLFYIIPKHSSCVLHCIQFYHEDHFKIIFEVSLEIIFVYQAVCLINLGFDPYEVKEQESSTSLNIQVFTDNTGGLCLFYLQPFKDAKEVKYMVIFLHRGCSKTFKVALSAEEMKQLKKMSFINLGGYVAVYLPDHFLHLINTRHPDLMCYHLFLADTDARIGEICCDGPVQSVLMSSVIEYCQGILFSVSLNHLLLLKYLWTCKRDCERLAVLHCFLLHLDQKPLWETEIIEWICENLSVCQMFDPMQEFIIASLYRRMSLETIYLDKLLPYTSLPFWNQDIEGVLWSSDITDMPILKIGMFKGFWEKFHSELEYMKHTHHNNPVHRRDWCKQIADVDTQEWRNIIYQRNILENAKKVILNMETWSSDQRIVPLHQEEDYLQKDLMWLMTIKLKDHLSQPLLHVGKNNIDKIVLDYISKQLDLICLILEVVWKKYNLDLYAFSFSRGCSSDYSAFYMMCHISESAIKLGMPLPPGFQTLHLVLGVRCLPLGNLLHYIDAGILQLTEAFAVKLLKELDDNESNEKLKYSIIVRLPERICQNIHRLWDHAISNNCIARKYVEQLLCKLKKRECHLAGQAACSQSALSMNFLPLNYLIKMLSTIEEQALNPFEEDNIDAAFLEEIALKQTTVLLRLHKS

Target ORF information:

RefSeq Version	XM_012960013.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960013.2

ORF Insert Sequence:

ATGTTACATC AGAGACTTTT TTACATTATT CCTAAGCATT CCAGTTGCGT CTTGCATTGC 
ATCCAGTTTT ATCATGAAGA TCATTTCAAA ATAATTTTTG AAGTTTCTCT TGAGATAATT 
TTCGTTTACC AAGCAGTATG TTTGATCAAT TTGGGCTTTG ACCCTTATGA AGTGAAAGAG 
CAAGAAAGTT CTACATCTCT AAACATACAG GTGTTTACAG ATAATACAGG AGGTCTGTGC 
TTGTTTTATC TCCAGCCTTT CAAAGATGCA AAGGAGGTGA AATACATGGT GATATTTTTG 
CACAGAGGTT GTAGTAAGAC CTTTAAAGTT GCATTATCCG CAGAAGAGAT GAAACAGCTA 
AAGAAAATGT CTTTTATTAA TCTTGGTGGT TATGTTGCTG TTTACTTGCC TGACCATTTT 
TTGCATTTGA TAAACACCAG GCATCCTGAC TTGATGTGCT ACCACTTGTT TTTGGCAGAT 
ACTGATGCCA GAATAGGTGA GATATGCTGT GATGGTCCTG TTCAGTCTGT TCTAATGTCG 
TCAGTGATTG AGTACTGCCA GGGGATTCTC TTCTCAGTCA GCCTAAACCA CTTGCTTCTT 
CTGAAATACC TTTGGACTTG TAAAAGGGAT TGTGAAAGGC TGGCAGTACT TCATTGTTTT 
CTTTTGCACC TGGATCAGAA GCCACTATGG GAAACAGAGA TTATTGAGTG GATATGTGAA 
AACCTCTCAG TATGCCAAAT GTTTGATCCC ATGCAGGAAT TTATAATTGC TTCTTTATAC 
AGAAGAATGA GTTTGGAAAC TATTTATCTA GATAAACTGC TGCCTTATAC TTCACTGCCT 
TTTTGGAATC AGGACATTGA GGGTGTGTTA TGGTCATCAG ACATCACAGA TATGCCAATA 
TTAAAGATTG GTATGTTTAA AGGATTCTGG GAGAAGTTCC ATTCTGAGCT GGAATATATG 
AAACATACTC ACCACAATAA CCCTGTGCAC CGAAGAGATT GGTGTAAACA AATTGCTGAT 
GTGGACACAC AAGAATGGAG GAATATTATT TATCAGCGCA ACATTTTGGA AAATGCAAAG 
AAAGTTATTT TAAACATGGA AACATGGAGT TCTGATCAGA GAATTGTGCC GCTTCATCAG 
GAAGAGGATT ATCTACAGAA AGACCTCATG TGGCTGATGA CGATTAAATT GAAGGATCAC 
CTGTCACAGC CTTTGCTTCA TGTTGGAAAA AATAACATTG ATAAGATTGT TCTGGATTAC 
ATTTCAAAGC AGTTAGACTT GATATGCCTG ATACTGGAAG TTGTGTGGAA AAAATACAAC 
TTGGATCTAT ATGCATTCTC TTTTTCAAGA GGATGTTCTT CAGACTATTC TGCCTTTTAC 
ATGATGTGCC ACATTTCAGA ATCTGCAATC AAACTGGGCA TGCCTCTTCC ACCTGGATTC 
CAGACACTAC ATTTGGTCCT GGGAGTTCGA TGCCTTCCTC TAGGAAATCT TCTGCATTAT 
ATAGATGCTG GAATTCTACA ACTGACTGAA GCTTTTGCTG TCAAACTTCT AAAAGAACTG 
GATGACAATG AAAGCAATGA AAAGTTAAAA TACAGCATTA TTGTACGTCT TCCTGAGAGA 
ATTTGCCAAA ATATTCATCG CTTATGGGAT CATGCCATTA GTAATAACTG CATCGCCAGA 
AAATATGTTG AACAATTATT GTGCAAGCTT AAAAAAAGGG AGTGTCACCT TGCAGGACAA 
GCAGCTTGTA GTCAGTCAGC ATTGTCCATG AATTTCTTGC CACTTAACTA CTTAATCAAG 
ATGCTGTCAA CAATAGAAGA ACAAGCATTA AATCCCTTTG AAGAAGATAA CATAGATGCT 
GCATTTCTGG AGGAAATCGC CTTAAAACAG ACTACTGTCC TTCTTAGGTT ACACAAAAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsap ( XM_012960014.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815468.1 Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa35978
Clone ID Related Accession (Same CDS sequence)	XM_012960014.2
Accession Version	XM_012960014.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gamma-secretase-activating protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACATC AGAGACTTTT TTACATTATT CCTAAGCATT CCAGTTGCGT CTTGCATTGC 
ATCCAGTTTT ATCATGAAGA TCATTTCAAA ATAATTTTTG AAGTTTCTCT TGAGATAATT 
TTCGTTTACC AAGCAGTATG TTTGATCAAT TTGGGCTTTG ACCCTTATGA AGTGAAAGAG 
CAAGAAAGTT CTACATCTCT AAACATACAG GTGTTTACAG ATAATACAGG AGGTCTGTGC 
TTGTTTTATC TCCAGCCTTT CAAAGATGCA AAGGAGGTGA AATACATGGT GATATTTTTG 
CACAGAGGTT GTAGTAAGAC CTTTAAAGTT GCATTATCCG CAGAAGAGAT GAAACAGCTA 
AAGAAAATGT CTTTTATTAA TCTTGGTGGT TATGTTGCTG TTTACTTGCC TGACCATTTT 
TTGCATTTGA TAAACACCAG GCATCCTGAC TTGATGTGCT ACCACTTGTT TTTGGCAGAT 
ACTGATGCCA GAATAGGTGA GATATGCTGT GATGGTCCTG TTCAGTCTGT TCTAATGTCG 
TCAGTGATTG AGTACTGCCA GGGGATTCTC TTCTCAGTCA GCCTAAACCA CTTGCTTCTT 
CTGAAATACC TTTGGACTTG TAAAAGGGAT TGTGAAAGGC TGGCAGTACT TCATTGTTTT 
CTTTTGCACC TGGATCAGAA GCCACTATGG GAAACAGAGA TTATTGAGTG GATATGTGAA 
AACCTCTCAG TATGCCAAAT GTTTGATCCC ATGCAGGAAT TTATAATTGC TTCTTTATAC 
AGAAGAATGA GTTTGGAAAC TATTTATCTA GATAAACTGC TGCCTTATAC TTCACTGCCT 
TTTTGGAATC AGGACATTGA GGGTGTGTTA TGGTCATCAG ACATCACAGA TATGCCAATA 
TTAAAGGACA CACAAGAATG GAGGAATATT ATTTATCAGC GCAACATTTT GGAAAATGCA 
AAGAAAGTTA TTTTAAACAT GGAAACATGG AGTTCTGATC AGAGAATTGT GCCGCTTCAT 
CAGGAAGAGG ATTATCTACA GAAAGACCTC ATGTGGCTGA TGACGATTAA ATTGAAGGAT 
CACCTGTCAC AGCCTTTGCT TCATGTTGGA AAAAATAACA TTGATAAGAT TGTTCTGGAT 
TACATTTCAA AGCAGTTAGA CTTGATATGC CTGATACTGG AAGTTGTGTG GAAAAAATAC 
AACTTGGATC TATATGCATT CTCTTTCCTA TTTATTTGCA GTAGTTCAAG AGGATGTTCT 
TCAGACTATT CTGCCTTTTA CATGATGTGC CACATTTCAG AATCTGCAAT CAAACTGGGC 
ATGCCTCTTC CACCTGGATT CCAGACACTA CATTTGGTCC TGGGAGTTCG ATGCCTTCCT 
CTAGGAAATC TTCTGCATTA TATAGATGCT GGAATTCTAC AACTGACTGA AGCTTTTGCT 
GTCAAACTTC TAAAAGAACT GGATGACAAT GAAAGCAATG AAAAGTTAAA ATACAGCATT 
ATTGTACGTC TTCCTGAGAG AATTTGCCAA AATATTCATC GCTTATGGGA TCATGCCATT 
AGTAATAACT GCATCGCCAG AAAATATGTT GAACAATTAT TGTGCAAGCT TAAAAAAAGG 
GAGTGTCACC TTGCAGGACA AGCAGCTTGT AGTCAGTCAG CATTGTCCAT GAATTTCTTG 
CCACTTAACT ACTTAATCAA GATGCTGTCA ACAATAGAAG AACAAGCATT AAATCCCTTT 
GAAGAAGATA ACATAGATGC TGCATTTCTG GAGGAAATCG CCTTAAAACA GACTACTGTC 
CTTCTTAGGT TACACAAAAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815468.1
CDS	4..1827
Translation	MLHQRLFYIIPKHSSCVLHCIQFYHEDHFKIIFEVSLEIIFVYQAVCLINLGFDPYEVKEQESSTSLNIQVFTDNTGGLCLFYLQPFKDAKEVKYMVIFLHRGCSKTFKVALSAEEMKQLKKMSFINLGGYVAVYLPDHFLHLINTRHPDLMCYHLFLADTDARIGEICCDGPVQSVLMSSVIEYCQGILFSVSLNHLLLLKYLWTCKRDCERLAVLHCFLLHLDQKPLWETEIIEWICENLSVCQMFDPMQEFIIASLYRRMSLETIYLDKLLPYTSLPFWNQDIEGVLWSSDITDMPILKDTQEWRNIIYQRNILENAKKVILNMETWSSDQRIVPLHQEEDYLQKDLMWLMTIKLKDHLSQPLLHVGKNNIDKIVLDYISKQLDLICLILEVVWKKYNLDLYAFSFLFICSSSRGCSSDYSAFYMMCHISESAIKLGMPLPPGFQTLHLVLGVRCLPLGNLLHYIDAGILQLTEAFAVKLLKELDDNESNEKLKYSIIVRLPERICQNIHRLWDHAISNNCIARKYVEQLLCKLKKRECHLAGQAACSQSALSMNFLPLNYLIKMLSTIEEQALNPFEEDNIDAAFLEEIALKQTTVLLRLHKS

Target ORF information:

RefSeq Version	XM_012960014.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960014.2

ORF Insert Sequence:

ATGTTACATC AGAGACTTTT TTACATTATT CCTAAGCATT CCAGTTGCGT CTTGCATTGC 
ATCCAGTTTT ATCATGAAGA TCATTTCAAA ATAATTTTTG AAGTTTCTCT TGAGATAATT 
TTCGTTTACC AAGCAGTATG TTTGATCAAT TTGGGCTTTG ACCCTTATGA AGTGAAAGAG 
CAAGAAAGTT CTACATCTCT AAACATACAG GTGTTTACAG ATAATACAGG AGGTCTGTGC 
TTGTTTTATC TCCAGCCTTT CAAAGATGCA AAGGAGGTGA AATACATGGT GATATTTTTG 
CACAGAGGTT GTAGTAAGAC CTTTAAAGTT GCATTATCCG CAGAAGAGAT GAAACAGCTA 
AAGAAAATGT CTTTTATTAA TCTTGGTGGT TATGTTGCTG TTTACTTGCC TGACCATTTT 
TTGCATTTGA TAAACACCAG GCATCCTGAC TTGATGTGCT ACCACTTGTT TTTGGCAGAT 
ACTGATGCCA GAATAGGTGA GATATGCTGT GATGGTCCTG TTCAGTCTGT TCTAATGTCG 
TCAGTGATTG AGTACTGCCA GGGGATTCTC TTCTCAGTCA GCCTAAACCA CTTGCTTCTT 
CTGAAATACC TTTGGACTTG TAAAAGGGAT TGTGAAAGGC TGGCAGTACT TCATTGTTTT 
CTTTTGCACC TGGATCAGAA GCCACTATGG GAAACAGAGA TTATTGAGTG GATATGTGAA 
AACCTCTCAG TATGCCAAAT GTTTGATCCC ATGCAGGAAT TTATAATTGC TTCTTTATAC 
AGAAGAATGA GTTTGGAAAC TATTTATCTA GATAAACTGC TGCCTTATAC TTCACTGCCT 
TTTTGGAATC AGGACATTGA GGGTGTGTTA TGGTCATCAG ACATCACAGA TATGCCAATA 
TTAAAGGACA CACAAGAATG GAGGAATATT ATTTATCAGC GCAACATTTT GGAAAATGCA 
AAGAAAGTTA TTTTAAACAT GGAAACATGG AGTTCTGATC AGAGAATTGT GCCGCTTCAT 
CAGGAAGAGG ATTATCTACA GAAAGACCTC ATGTGGCTGA TGACGATTAA ATTGAAGGAT 
CACCTGTCAC AGCCTTTGCT TCATGTTGGA AAAAATAACA TTGATAAGAT TGTTCTGGAT 
TACATTTCAA AGCAGTTAGA CTTGATATGC CTGATACTGG AAGTTGTGTG GAAAAAATAC 
AACTTGGATC TATATGCATT CTCTTTCCTA TTTATTTGCA GTAGTTCAAG AGGATGTTCT 
TCAGACTATT CTGCCTTTTA CATGATGTGC CACATTTCAG AATCTGCAAT CAAACTGGGC 
ATGCCTCTTC CACCTGGATT CCAGACACTA CATTTGGTCC TGGGAGTTCG ATGCCTTCCT 
CTAGGAAATC TTCTGCATTA TATAGATGCT GGAATTCTAC AACTGACTGA AGCTTTTGCT 
GTCAAACTTC TAAAAGAACT GGATGACAAT GAAAGCAATG AAAAGTTAAA ATACAGCATT 
ATTGTACGTC TTCCTGAGAG AATTTGCCAA AATATTCATC GCTTATGGGA TCATGCCATT 
AGTAATAACT GCATCGCCAG AAAATATGTT GAACAATTAT TGTGCAAGCT TAAAAAAAGG 
GAGTGTCACC TTGCAGGACA AGCAGCTTGT AGTCAGTCAG CATTGTCCAT GAATTTCTTG 
CCACTTAACT ACTTAATCAA GATGCTGTCA ACAATAGAAG AACAAGCATT AAATCCCTTT 
GAAGAAGATA ACATAGATGC TGCATTTCTG GAGGAAATCG CCTTAAAACA GACTACTGTC 
CTTCTTAGGT TACACAAAAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsap ( XM_012960012.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815466.2 Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa54861
Clone ID Related Accession (Same CDS sequence)	XM_012960012.3
Accession Version	XM_012960012.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gamma-secretase-activating protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960012.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG ATTTCTGTGC AAGTTTTTCT CTGCAGAGAG ATGTCATGCC GTGGATCCTG 
GCCCGGGAAG CCTCTGAAAT TTCAAAGAGG AACTCAAGGA CCCTCCGTAT TGTGAATGTT 
GAGCGAAATG GAAGAGTTCT CTTTACCTGG AAGGGAGGCG ATGGTCTTAC CAACATTGGT 
TTGTATGATC CTGATTTAAG ACAAAATGAG ATGCTTTATT CATTTGACAA AGATCTACTT 
ATTATAAGCT GTTCAGTGAA CTGTGAAAAA AGTCTGCTTG CATTAAGTTA CTGTAATTCA 
GTCAAAGAAC ATCAATATGA ACCTCTTAGA ACAGTTTCCA AATACTTGGC ATTACTGATT 
GAAATAAAAC CAGTCAACAA TGTCAGAGTT CTAAAAGCTG TGGACTATGA CATTAGAGTA 
CAGTTCTTAT ATCCAACTGA AGACGTTTGT CCATTTCCTG AAAGTCATCT TTTGTTGGTT 
TCGAAAGAGA AGTATATTGA ACAATTTCAT GTTATACTTA CTGTTGAAGG TGACAGTAGT 
GTGGTTATCA AAAATTCTGG CAAGCTTCCA AGGGAAAGAA TAGCAGAAGA CTTTGTATGG 
GTTCAGTGGG ATATGTTACA TCAGAGACTT TTTTACATTA TTCCTAAGCA TTCCAGTTGC 
GTCTTGCATT GCATCCAGTT TTATCATGAA GATCATTTCA AAATAATTTT TGAAGTTTCT 
CTTGAGATAA TTTTCGTTTA CCAAGCAGTA TGTTTGATCA ATTTGGGCTT TGACCCTTAT 
GAAGTGAAAG AGCAAGAAAG TTCTACATCT CTAAACATAC AGGTGTTTAC AGATAATACA 
GGAGGTCTGT GCTTGTTTTA TCTCCAGCCT TTCAAAGATG CAAAGGAGGT GAAATACATG 
GTGATATTTT TGCACAGAGG TTGTAGTAAG ACCTTTAAAG TTGCATTATC CGCAGAAGAG 
ATGAAACAGC TAAAGAAAAT GTCTTTTATT AATCTTGGTG GTTATGTTGC TGTTTACTTG 
CCTGACCATT TTTTGCATTT GATAAACACC AGGCATCCTG ACTTGATGTG CTACCACTTG 
TTTTTGGCAG ATACTGATGC CAGAATAGGT GAGATATGCT GTGATGGTCC TGTTCAGTCT 
GTTCTAATGT CGTCAGTGAT TGAGTACTGC CAGGGGATTC TCTTCTCAGT CAGCCTAAAC 
CACTTGCTTC TTCTGAAATA CCTTTGGACT TGTAAAAGGG ATTGTGAAAG GCTGGCAGTA 
CTTCATTGTT TTCTTTTGCA CCTGGATCAG AAGCCACTAT GGGAAACAGA GATTATTGAG 
TGGATATGTG AAAACCTCTC AGTATGCCAA ATGTTTGATC CCATACAGGA ATTTATAATT 
GCTTCTTTAT ACAGAAGAAT GAGTTTGGAA ACTATTTATC TAGATAAACT GCTGCCTTAT 
ACTTCACTGC CTTTTTGGAA TCAGGGCATT GAGGGTGTGT TATGGTCATC AGACATCACA 
GATATGCCAA TATTAAAGAT TGGTATGTTT AAAGGATTCT GGGAGAAGTT CCATTCTGAG 
CTGGAATATA TGAAACATAC TCACCACAAT AACCCTGTGC ACCGAAGAGA TTGGTGTAAA 
CAAATTGCTG ATGTGGACAC ACAAGAATGG AGGAATATTA TTTATCAGCG CAACATTTTG 
GAAAATGCAA AAAAAGTTAT TTTAAACATG GAAACATGGA GTTCTGATCA GAGAATTGTG 
CCGCTTCATC AGGAAGAGGA TTATCTACAG AAAGACCTCA TGTGGCTGAT GACGATTAAA 
TTGAAGGATC ACCTGTCACA GCCTTTGCTT CATGTTGGAA AAAATAACAT TGATAAGATT 
GTTCTGGATT ACATTTCAAA GCAGTTAGAC TTGATATGCC TGATACTGGA AGTTGTGTGG 
AAAAAATACA ACTTGGATCT ATATGCATTC TCTTTCCTAT TTATTTGTAG TAGTTCAAGA 
GGATGTTCTT CAGACTATTC TGCCTTTTAC ATGATGTGCC ACATTTCAGA ATCTGCAATC 
AAACTGGGCA TGCCTCTTCC ACCTGGATTC CAGACACTAC ATTTGGTCCT GGGAGTTCGA 
TGCCTTCCTC TAGGAAATCT TCTGCATTAT ATAGATGCTG GAATTCTACA ACTGACTGAA 
GCTTTTGCTG TCAAACTTCT AAAAGAACTG GATGACAATG AAAGCAATGA AAAGTTAAAA 
TACAGCATTA TTGTACGTCT TCCTGAGAGA ATTTGCCAAA ATATTCATCG CTTATGGGAT 
CATGCCATTA GTAATAACTG CATCGCCAGA AAATATGTTG AACAATTATT GTGCAAGCTT 
AAAAAAAGGG AGTGTCACCT TGCAGGACAA GCAGCTTGTA GTCAGTCAGC ATTGTCCATG 
AATTTCTTGC CACTTAACTA CTTAATCAAG ATGCTGTCAA CAATAGAAGA ACAAGCATTA 
AATCCCTTTG AAGAAGATAA CATAGATGCT GCATTTCTGG AGGAAATCGC CTTAAAACAG 
ACTACTGTCC TTCTTAGGTT ACACAAAAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815466.2
CDS	497..3049
Translation	MVLDFCASFSLQRDVMPWILAREASEISKRNSRTLRIVNVERNGRVLFTWKGGDGLTNIGLYDPDLRQNEMLYSFDKDLLIISCSVNCEKSLLALSYCNSVKEHQYEPLRTVSKYLALLIEIKPVNNVRVLKAVDYDIRVQFLYPTEDVCPFPESHLLLVSKEKYIEQFHVILTVEGDSSVVIKNSGKLPRERIAEDFVWVQWDMLHQRLFYIIPKHSSCVLHCIQFYHEDHFKIIFEVSLEIIFVYQAVCLINLGFDPYEVKEQESSTSLNIQVFTDNTGGLCLFYLQPFKDAKEVKYMVIFLHRGCSKTFKVALSAEEMKQLKKMSFINLGGYVAVYLPDHFLHLINTRHPDLMCYHLFLADTDARIGEICCDGPVQSVLMSSVIEYCQGILFSVSLNHLLLLKYLWTCKRDCERLAVLHCFLLHLDQKPLWETEIIEWICENLSVCQMFDPIQEFIIASLYRRMSLETIYLDKLLPYTSLPFWNQGIEGVLWSSDITDMPILKIGMFKGFWEKFHSELEYMKHTHHNNPVHRRDWCKQIADVDTQEWRNIIYQRNILENAKKVILNMETWSSDQRIVPLHQEEDYLQKDLMWLMTIKLKDHLSQPLLHVGKNNIDKIVLDYISKQLDLICLILEVVWKKYNLDLYAFSFLFICSSSRGCSSDYSAFYMMCHISESAIKLGMPLPPGFQTLHLVLGVRCLPLGNLLHYIDAGILQLTEAFAVKLLKELDDNESNEKLKYSIIVRLPERICQNIHRLWDHAISNNCIARKYVEQLLCKLKKRECHLAGQAACSQSALSMNFLPLNYLIKMLSTIEEQALNPFEEDNIDAAFLEEIALKQTTVLLRLHKS

Target ORF information:

RefSeq Version	XM_012960012.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960012.3

ORF Insert Sequence:

ATGGTCCTGG ATTTCTGTGC AAGTTTTTCT CTGCAGAGAG ATGTCATGCC GTGGATCCTG 
GCCCGGGAAG CCTCTGAAAT TTCAAAGAGG AACTCAAGGA CCCTCCGTAT TGTGAATGTT 
GAGCGAAATG GAAGAGTTCT CTTTACCTGG AAGGGAGGCG ATGGTCTTAC CAACATTGGT 
TTGTATGATC CTGATTTAAG ACAAAATGAG ATGCTTTATT CATTTGACAA AGATCTACTT 
ATTATAAGCT GTTCAGTGAA CTGTGAAAAA AGTCTGCTTG CATTAAGTTA CTGTAATTCA 
GTCAAAGAAC ATCAATATGA ACCTCTTAGA ACAGTTTCCA AATACTTGGC ATTACTGATT 
GAAATAAAAC CAGTCAACAA TGTCAGAGTT CTAAAAGCTG TGGACTATGA CATTAGAGTA 
CAGTTCTTAT ATCCAACTGA AGACGTTTGT CCATTTCCTG AAAGTCATCT TTTGTTGGTT 
TCGAAAGAGA AGTATATTGA ACAATTTCAT GTTATACTTA CTGTTGAAGG TGACAGTAGT 
GTGGTTATCA AAAATTCTGG CAAGCTTCCA AGGGAAAGAA TAGCAGAAGA CTTTGTATGG 
GTTCAGTGGG ATATGTTACA TCAGAGACTT TTTTACATTA TTCCTAAGCA TTCCAGTTGC 
GTCTTGCATT GCATCCAGTT TTATCATGAA GATCATTTCA AAATAATTTT TGAAGTTTCT 
CTTGAGATAA TTTTCGTTTA CCAAGCAGTA TGTTTGATCA ATTTGGGCTT TGACCCTTAT 
GAAGTGAAAG AGCAAGAAAG TTCTACATCT CTAAACATAC AGGTGTTTAC AGATAATACA 
GGAGGTCTGT GCTTGTTTTA TCTCCAGCCT TTCAAAGATG CAAAGGAGGT GAAATACATG 
GTGATATTTT TGCACAGAGG TTGTAGTAAG ACCTTTAAAG TTGCATTATC CGCAGAAGAG 
ATGAAACAGC TAAAGAAAAT GTCTTTTATT AATCTTGGTG GTTATGTTGC TGTTTACTTG 
CCTGACCATT TTTTGCATTT GATAAACACC AGGCATCCTG ACTTGATGTG CTACCACTTG 
TTTTTGGCAG ATACTGATGC CAGAATAGGT GAGATATGCT GTGATGGTCC TGTTCAGTCT 
GTTCTAATGT CGTCAGTGAT TGAGTACTGC CAGGGGATTC TCTTCTCAGT CAGCCTAAAC 
CACTTGCTTC TTCTGAAATA CCTTTGGACT TGTAAAAGGG ATTGTGAAAG GCTGGCAGTA 
CTTCATTGTT TTCTTTTGCA CCTGGATCAG AAGCCACTAT GGGAAACAGA GATTATTGAG 
TGGATATGTG AAAACCTCTC AGTATGCCAA ATGTTTGATC CCATACAGGA ATTTATAATT 
GCTTCTTTAT ACAGAAGAAT GAGTTTGGAA ACTATTTATC TAGATAAACT GCTGCCTTAT 
ACTTCACTGC CTTTTTGGAA TCAGGGCATT GAGGGTGTGT TATGGTCATC AGACATCACA 
GATATGCCAA TATTAAAGAT TGGTATGTTT AAAGGATTCT GGGAGAAGTT CCATTCTGAG 
CTGGAATATA TGAAACATAC TCACCACAAT AACCCTGTGC ACCGAAGAGA TTGGTGTAAA 
CAAATTGCTG ATGTGGACAC ACAAGAATGG AGGAATATTA TTTATCAGCG CAACATTTTG 
GAAAATGCAA AAAAAGTTAT TTTAAACATG GAAACATGGA GTTCTGATCA GAGAATTGTG 
CCGCTTCATC AGGAAGAGGA TTATCTACAG AAAGACCTCA TGTGGCTGAT GACGATTAAA 
TTGAAGGATC ACCTGTCACA GCCTTTGCTT CATGTTGGAA AAAATAACAT TGATAAGATT 
GTTCTGGATT ACATTTCAAA GCAGTTAGAC TTGATATGCC TGATACTGGA AGTTGTGTGG 
AAAAAATACA ACTTGGATCT ATATGCATTC TCTTTCCTAT TTATTTGTAG TAGTTCAAGA 
GGATGTTCTT CAGACTATTC TGCCTTTTAC ATGATGTGCC ACATTTCAGA ATCTGCAATC 
AAACTGGGCA TGCCTCTTCC ACCTGGATTC CAGACACTAC ATTTGGTCCT GGGAGTTCGA 
TGCCTTCCTC TAGGAAATCT TCTGCATTAT ATAGATGCTG GAATTCTACA ACTGACTGAA 
GCTTTTGCTG TCAAACTTCT AAAAGAACTG GATGACAATG AAAGCAATGA AAAGTTAAAA 
TACAGCATTA TTGTACGTCT TCCTGAGAGA ATTTGCCAAA ATATTCATCG CTTATGGGAT 
CATGCCATTA GTAATAACTG CATCGCCAGA AAATATGTTG AACAATTATT GTGCAAGCTT 
AAAAAAAGGG AGTGTCACCT TGCAGGACAA GCAGCTTGTA GTCAGTCAGC ATTGTCCATG 
AATTTCTTGC CACTTAACTA CTTAATCAAG ATGCTGTCAA CAATAGAAGA ACAAGCATTA 
AATCCCTTTG AAGAAGATAA CATAGATGCT GCATTTCTGG AGGAAATCGC CTTAAAACAG 
ACTACTGTCC TTCTTAGGTT ACACAAAAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsap ( XM_004913025.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913082.2 Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa54862
Clone ID Related Accession (Same CDS sequence)	XM_004913025.4
Accession Version	XM_004913025.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gamma-secretase-activating protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913025.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG ATTTCTGTGC AAGTTTTTCT CTGCAGAGAG ATGTCATGCC GTGGATCCTG 
GCCCGGGAAG CCTCTGAAAT TTCAAAGAGG AACTCAAGGA CCCTCCGTAT TGTGAATGTT 
GAGCGAAATG GAAGAGTTCT CTTTACCTGG AAGGGAGGCG ATGGTCTTAC CAACATTGGT 
TTGTATGATC CTGATTTAAG ACAAAATGAG ATGCTTTATT CATTTGACAA AGATCTACTT 
ATTATAAGCT GTTCAGTGAA CTGTGAAAAA AGTCTGCTTG CATTAAGTTA CTGTAATTCA 
GTCAAAGAAC ATCAATATGA ACCTCTTAGA ACAGTTTCCA AATACTTGGC ATTACTGATT 
GAAATAAAAC CAGTCAACAA TGTCAGAGTT CTAAAAGCTG TGGACTATGA CATTAGAGTA 
CAGTTCTTAT ATCCAACTGA AGACGTTTGT CCATTTCCTG AAAGTCATCT TTTGTTGGTT 
TCGAAAGAGA AGTATATTGA ACAATTTCAT GTTATACTTA CTGTTGAAGG TGACAGTAGT 
GTGGTTATCA AAAATTCTGG CAAGCTTCCA AGGGAAAGAA TAGCAGAAGA CTTTGTATGG 
GTTCAGTGGG ATATGTTACA TCAGAGACTT TTTTACATTA TTCCTAAGCA TTCCAGTTGC 
GTCTTGCATT GCATCCAGTT TTATCATGAA GATCATTTCA AAATAATTTT TGAAGTTTCT 
CTTGAGATAA TTTTCGTTTA CCAAGCAGTA TGTTTGATCA ATTTGGGCTT TGACCCTTAT 
GAAGTGAAAG AGCAAGAAAG TTCTACATCT CTAAACATAC AGGTGTTTAC AGATAATACA 
GGAGGTCTGT GCTTGTTTTA TCTCCAGCCT TTCAAAGATG CAAAGGAGGT GAAATACATG 
GTGATATTTT TGCACAGAGG TTGTAGTAAG ACCTTTAAAG TTGCATTATC CGCAGAAGAG 
ATGAAACAGC TAAAGAAAAT GTCTTTTATT AATCTTGGTG GTTATGTTGC TGTTTACTTG 
CCTGACCATT TTTTGCATTT GATAAACACC AGGCATCCTG ACTTGATGTG CTACCACTTG 
TTTTTGGCAG ATACTGATGC CAGAATAGGT GAGATATGCT GTGATGGTCC TGTTCAGTCT 
GTTCTAATGT CGTCAGTGAT TGAGTACTGC CAGGGGATTC TCTTCTCAGT CAGCCTAAAC 
CACTTGCTTC TTCTGAAATA CCTTTGGACT TGTAAAAGGG ATTGTGAAAG GCTGGCAGTA 
CTTCATTGTT TTCTTTTGCA CCTGGATCAG AAGCCACTAT GGGAAACAGA GATTATTGAG 
TGGATATGTG AAAACCTCTC AGTATGCCAA ATGTTTGATC CCATACAGGA ATTTATAATT 
GCTTCTTTAT ACAGAAGAAT GAGTTTGGAA ACTATTTATC TAGATAAACT GCTGCCTTAT 
ACTTCACTGC CTTTTTGGAA TCAGGGCATT GAGGGTGTGT TATGGTCATC AGACATCACA 
GATATGCCAA TATTAAAGAT TGGTATGTTT AAAGGATTCT GGGAGAAGTT CCATTCTGAG 
CTGGAATATA TGAAACATAC TCACCACAAT AACCCTGTGC ACCGAAGAGA TTGGTGTAAA 
CAAATTGCTG ATGTGGACAC ACAAGAATGG AGGAATATTA TTTATCAGCG CAACATTTTG 
GAAAATGCAA AAAAAGTTAT TTTAAACATG GAAACATGGA GTTCTGATCA GAGAATTGTG 
CCGCTTCATC AGGAAGAGGA TTATCTACAG AAAGACCTCA TGTGGCTGAT GACGATTAAA 
TTGAAGGATC ACCTGTCACA GCCTTTGCTT CATGTTGGAA AAAATAACAT TGATAAGATT 
GTTCTGGATT ACATTTCAAA GCAGTTAGAC TTGATATGCC TGATACTGGA AGTTGTGTGG 
AAAAAATACA ACTTGGATCT ATATGCATTC TCTTTTAGTT CAAGAGGATG TTCTTCAGAC 
TATTCTGCCT TTTACATGAT GTGCCACATT TCAGAATCTG CAATCAAACT GGGCATGCCT 
CTTCCACCTG GATTCCAGAC ACTACATTTG GTCCTGGGAG TTCGATGCCT TCCTCTAGGA 
AATCTTCTGC ATTATATAGA TGCTGGAATT CTACAACTGA CTGAAGCTTT TGCTGTCAAA 
CTTCTAAAAG AACTGGATGA CAATGAAAGC AATGAAAAGT TAAAATACAG CATTATTGTA 
CGTCTTCCTG AGAGAATTTG CCAAAATATT CATCGCTTAT GGGATCATGC CATTAGTAAT 
AACTGCATCG CCAGAAAATA TGTTGAACAA TTATTGTGCA AGCTTAAAAA AAGGGAGTGT 
CACCTTGCAG GACAAGCAGC TTGTAGTCAG TCAGCATTGT CCATGAATTT CTTGCCACTT 
AACTACTTAA TCAAGATGCT GTCAACAATA GAAGAACAAG CATTAAATCC CTTTGAAGAA 
GATAACATAG ATGCTGCATT TCTGGAGGAA ATCGCCTTAA AACAGACTAC TGTCCTTCTT 
AGGTTACACA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913082.2
CDS	497..3034
Translation	MVLDFCASFSLQRDVMPWILAREASEISKRNSRTLRIVNVERNGRVLFTWKGGDGLTNIGLYDPDLRQNEMLYSFDKDLLIISCSVNCEKSLLALSYCNSVKEHQYEPLRTVSKYLALLIEIKPVNNVRVLKAVDYDIRVQFLYPTEDVCPFPESHLLLVSKEKYIEQFHVILTVEGDSSVVIKNSGKLPRERIAEDFVWVQWDMLHQRLFYIIPKHSSCVLHCIQFYHEDHFKIIFEVSLEIIFVYQAVCLINLGFDPYEVKEQESSTSLNIQVFTDNTGGLCLFYLQPFKDAKEVKYMVIFLHRGCSKTFKVALSAEEMKQLKKMSFINLGGYVAVYLPDHFLHLINTRHPDLMCYHLFLADTDARIGEICCDGPVQSVLMSSVIEYCQGILFSVSLNHLLLLKYLWTCKRDCERLAVLHCFLLHLDQKPLWETEIIEWICENLSVCQMFDPIQEFIIASLYRRMSLETIYLDKLLPYTSLPFWNQGIEGVLWSSDITDMPILKIGMFKGFWEKFHSELEYMKHTHHNNPVHRRDWCKQIADVDTQEWRNIIYQRNILENAKKVILNMETWSSDQRIVPLHQEEDYLQKDLMWLMTIKLKDHLSQPLLHVGKNNIDKIVLDYISKQLDLICLILEVVWKKYNLDLYAFSFSSRGCSSDYSAFYMMCHISESAIKLGMPLPPGFQTLHLVLGVRCLPLGNLLHYIDAGILQLTEAFAVKLLKELDDNESNEKLKYSIIVRLPERICQNIHRLWDHAISNNCIARKYVEQLLCKLKKRECHLAGQAACSQSALSMNFLPLNYLIKMLSTIEEQALNPFEEDNIDAAFLEEIALKQTTVLLRLHKS

Target ORF information:

RefSeq Version	XM_004913025.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913025.4

ORF Insert Sequence:

ATGGTCCTGG ATTTCTGTGC AAGTTTTTCT CTGCAGAGAG ATGTCATGCC GTGGATCCTG 
GCCCGGGAAG CCTCTGAAAT TTCAAAGAGG AACTCAAGGA CCCTCCGTAT TGTGAATGTT 
GAGCGAAATG GAAGAGTTCT CTTTACCTGG AAGGGAGGCG ATGGTCTTAC CAACATTGGT 
TTGTATGATC CTGATTTAAG ACAAAATGAG ATGCTTTATT CATTTGACAA AGATCTACTT 
ATTATAAGCT GTTCAGTGAA CTGTGAAAAA AGTCTGCTTG CATTAAGTTA CTGTAATTCA 
GTCAAAGAAC ATCAATATGA ACCTCTTAGA ACAGTTTCCA AATACTTGGC ATTACTGATT 
GAAATAAAAC CAGTCAACAA TGTCAGAGTT CTAAAAGCTG TGGACTATGA CATTAGAGTA 
CAGTTCTTAT ATCCAACTGA AGACGTTTGT CCATTTCCTG AAAGTCATCT TTTGTTGGTT 
TCGAAAGAGA AGTATATTGA ACAATTTCAT GTTATACTTA CTGTTGAAGG TGACAGTAGT 
GTGGTTATCA AAAATTCTGG CAAGCTTCCA AGGGAAAGAA TAGCAGAAGA CTTTGTATGG 
GTTCAGTGGG ATATGTTACA TCAGAGACTT TTTTACATTA TTCCTAAGCA TTCCAGTTGC 
GTCTTGCATT GCATCCAGTT TTATCATGAA GATCATTTCA AAATAATTTT TGAAGTTTCT 
CTTGAGATAA TTTTCGTTTA CCAAGCAGTA TGTTTGATCA ATTTGGGCTT TGACCCTTAT 
GAAGTGAAAG AGCAAGAAAG TTCTACATCT CTAAACATAC AGGTGTTTAC AGATAATACA 
GGAGGTCTGT GCTTGTTTTA TCTCCAGCCT TTCAAAGATG CAAAGGAGGT GAAATACATG 
GTGATATTTT TGCACAGAGG TTGTAGTAAG ACCTTTAAAG TTGCATTATC CGCAGAAGAG 
ATGAAACAGC TAAAGAAAAT GTCTTTTATT AATCTTGGTG GTTATGTTGC TGTTTACTTG 
CCTGACCATT TTTTGCATTT GATAAACACC AGGCATCCTG ACTTGATGTG CTACCACTTG 
TTTTTGGCAG ATACTGATGC CAGAATAGGT GAGATATGCT GTGATGGTCC TGTTCAGTCT 
GTTCTAATGT CGTCAGTGAT TGAGTACTGC CAGGGGATTC TCTTCTCAGT CAGCCTAAAC 
CACTTGCTTC TTCTGAAATA CCTTTGGACT TGTAAAAGGG ATTGTGAAAG GCTGGCAGTA 
CTTCATTGTT TTCTTTTGCA CCTGGATCAG AAGCCACTAT GGGAAACAGA GATTATTGAG 
TGGATATGTG AAAACCTCTC AGTATGCCAA ATGTTTGATC CCATACAGGA ATTTATAATT 
GCTTCTTTAT ACAGAAGAAT GAGTTTGGAA ACTATTTATC TAGATAAACT GCTGCCTTAT 
ACTTCACTGC CTTTTTGGAA TCAGGGCATT GAGGGTGTGT TATGGTCATC AGACATCACA 
GATATGCCAA TATTAAAGAT TGGTATGTTT AAAGGATTCT GGGAGAAGTT CCATTCTGAG 
CTGGAATATA TGAAACATAC TCACCACAAT AACCCTGTGC ACCGAAGAGA TTGGTGTAAA 
CAAATTGCTG ATGTGGACAC ACAAGAATGG AGGAATATTA TTTATCAGCG CAACATTTTG 
GAAAATGCAA AAAAAGTTAT TTTAAACATG GAAACATGGA GTTCTGATCA GAGAATTGTG 
CCGCTTCATC AGGAAGAGGA TTATCTACAG AAAGACCTCA TGTGGCTGAT GACGATTAAA 
TTGAAGGATC ACCTGTCACA GCCTTTGCTT CATGTTGGAA AAAATAACAT TGATAAGATT 
GTTCTGGATT ACATTTCAAA GCAGTTAGAC TTGATATGCC TGATACTGGA AGTTGTGTGG 
AAAAAATACA ACTTGGATCT ATATGCATTC TCTTTTAGTT CAAGAGGATG TTCTTCAGAC 
TATTCTGCCT TTTACATGAT GTGCCACATT TCAGAATCTG CAATCAAACT GGGCATGCCT 
CTTCCACCTG GATTCCAGAC ACTACATTTG GTCCTGGGAG TTCGATGCCT TCCTCTAGGA 
AATCTTCTGC ATTATATAGA TGCTGGAATT CTACAACTGA CTGAAGCTTT TGCTGTCAAA 
CTTCTAAAAG AACTGGATGA CAATGAAAGC AATGAAAAGT TAAAATACAG CATTATTGTA 
CGTCTTCCTG AGAGAATTTG CCAAAATATT CATCGCTTAT GGGATCATGC CATTAGTAAT 
AACTGCATCG CCAGAAAATA TGTTGAACAA TTATTGTGCA AGCTTAAAAA AAGGGAGTGT 
CACCTTGCAG GACAAGCAGC TTGTAGTCAG TCAGCATTGT CCATGAATTT CTTGCCACTT 
AACTACTTAA TCAAGATGCT GTCAACAATA GAAGAACAAG CATTAAATCC CTTTGAAGAA 
GATAACATAG ATGCTGCATT TCTGGAGGAA ATCGCCTTAA AACAGACTAC TGTCCTTCTT 
AGGTTACACA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsap ( XM_012960013.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815467.2 Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa54863
Clone ID Related Accession (Same CDS sequence)	XM_012960013.3
Accession Version	XM_012960013.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gamma-secretase-activating protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960013.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG ATTTCTGTGC AAGTTTTTCT CTGCAGAGAG ATGTCATGCC GTGGATCCTG 
GCCCGGGAAG CCTCTGAAAT TTCAAAGAGG AACTCAAGGA CCCTCCGTAT TGTGAATGTT 
GAGCGAAATG GAAGAGTTCT CTTTACCTGG AAGGGAGGCG ATGGTCTTAC CAACATTGGT 
TTGTATGATC CTGATTTAAG ACAAAATGAG ATGCTTTATT CATTTGACAA AGATCTACTT 
ATTATAAGCT GTTCAGTGAA CTGTGAAAAA AGTCTGCTTG CATTAAGTTA CTGTAATTCA 
GTCAAAGAAC ATCAATATGA ACCTCTTAGA ACAGTTTCCA AATACTTGGC ATTACTGATT 
GAAATAAAAC CAGTCAACAA TGTCAGAGTT CTAAAAGCTG TGGACTATGA CATTAGAGTA 
CAGTTCTTAT ATCCAACTGA AGACGTTTGT CCATTTCCTG AAAGTCATCT TTTGTTGGTT 
TCGAAAGAGA AGTATATTGA ACAATTTCAT GTTATACTTA CTGTTGAAGG TGACAGTAGT 
GTGGTTATCA AAAATTCTGG CAAGCTTCCA AGGGAAAGAA TAGCAGAAGA CTTTGTATGG 
GTTCAGTGGG ATATGTTACA TCAGAGACTT TTTTACATTA TTCCTAAGCA TTCCAGTTGC 
GTCTTGCATT GCATCCAGTT TTATCATGAA GATCATTTCA AAATAATTTT TGAAGTTTCT 
CTTGAGATAA TTTTCGTTTA CCAAGCAGTA TGTTTGATCA ATTTGGGCTT TGACCCTTAT 
GAAGTGAAAG AGCAAGAAAG TTCTACATCT CTAAACATAC AGGTGTTTAC AGATAATACA 
GGAGGTCTGT GCTTGTTTTA TCTCCAGCCT TTCAAAGATG CAAAGGAGGT GAAATACATG 
GTGATATTTT TGCACAGAGG TTGTAGTAAG ACCTTTAAAG TTGCATTATC CGCAGAAGAG 
ATGAAACAGC TAAAGAAAAT GTCTTTTATT AATCTTGGTG GTTATGTTGC TGTTTACTTG 
CCTGACCATT TTTTGCATTT GATAAACACC AGGCATCCTG ACTTGATGTG CTACCACTTG 
TTTTTGGCAG ATACTGATGC CAGAATAGGT GAGATATGCT GTGATGGTCC TGTTCAGTCT 
GTTCTAATGT CGTCAGTGAT TGAGTACTGC CAGGGGATTC TCTTCTCAGT CAGCCTAAAC 
CACTTGCTTC TTCTGAAATA CCTTTGGACT TGTAAAAGGG ATTGTGAAAG GCTGGCAGTA 
CTTCATTGTT TTCTTTTGCA CCTGGATCAG AAGCCACTAT GGGAAACAGA GATTATTGAG 
TGGATATGTG AAAACCTCTC AGTATGCCAA ATGTTTGATC CCATACAGGA ATTTATAATT 
GCTTCTTTAT ACAGAAGAAT GAGTTTGGAA ACTATTTATC TAGATAAACT GCTGCCTTAT 
ACTTCACTGC CTTTTTGGAA TCAGGGCATT GAGGGTGTGT TATGGTCATC AGACATCACA 
GATATGCCAA TATTAAAGAT TGGTATGTTT AAAGGATTCT GGGAGAAGTT CCATTCTGAG 
CTGGAATATA TGAAACATAC TCACCACAAT AACCCTGTGC ACCGAAGAGA TTGGTGTAAA 
CAAATTGCTG ATGTGGACAC ACAAGAATGG AGGAATATTA TTTATCAGCG CAACATTTTG 
GAAAATGCAA AAAAAGTTAT TTTAAACATG GAAACATGGA GTTCTGATCA GAGAATTGTG 
CCGCTTCATC AGGAAGAGGA TTATCTACAG AAAGACCTCA TGTGGCTGAT GACGATTAAA 
TTGAAGGATC ACCTGTCACA GCCTTTGCTT CATGTTGGAA AAAATAACAT TGATAAGATT 
GTTCTGGATT ACATTTCAAA GCAGTTAGAC TTGATATGCC TGATACTGGA AGTTGTGTGG 
AAAAAATACA ACTTGGATCT ATATGCATTC TCTTTTTCAA GAGGATGTTC TTCAGACTAT 
TCTGCCTTTT ACATGATGTG CCACATTTCA GAATCTGCAA TCAAACTGGG CATGCCTCTT 
CCACCTGGAT TCCAGACACT ACATTTGGTC CTGGGAGTTC GATGCCTTCC TCTAGGAAAT 
CTTCTGCATT ATATAGATGC TGGAATTCTA CAACTGACTG AAGCTTTTGC TGTCAAACTT 
CTAAAAGAAC TGGATGACAA TGAAAGCAAT GAAAAGTTAA AATACAGCAT TATTGTACGT 
CTTCCTGAGA GAATTTGCCA AAATATTCAT CGCTTATGGG ATCATGCCAT TAGTAATAAC 
TGCATCGCCA GAAAATATGT TGAACAATTA TTGTGCAAGC TTAAAAAAAG GGAGTGTCAC 
CTTGCAGGAC AAGCAGCTTG TAGTCAGTCA GCATTGTCCA TGAATTTCTT GCCACTTAAC 
TACTTAATCA AGATGCTGTC AACAATAGAA GAACAAGCAT TAAATCCCTT TGAAGAAGAT 
AACATAGATG CTGCATTTCT GGAGGAAATC GCCTTAAAAC AGACTACTGT CCTTCTTAGG 
TTACACAAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815467.2
CDS	497..3031
Translation	MVLDFCASFSLQRDVMPWILAREASEISKRNSRTLRIVNVERNGRVLFTWKGGDGLTNIGLYDPDLRQNEMLYSFDKDLLIISCSVNCEKSLLALSYCNSVKEHQYEPLRTVSKYLALLIEIKPVNNVRVLKAVDYDIRVQFLYPTEDVCPFPESHLLLVSKEKYIEQFHVILTVEGDSSVVIKNSGKLPRERIAEDFVWVQWDMLHQRLFYIIPKHSSCVLHCIQFYHEDHFKIIFEVSLEIIFVYQAVCLINLGFDPYEVKEQESSTSLNIQVFTDNTGGLCLFYLQPFKDAKEVKYMVIFLHRGCSKTFKVALSAEEMKQLKKMSFINLGGYVAVYLPDHFLHLINTRHPDLMCYHLFLADTDARIGEICCDGPVQSVLMSSVIEYCQGILFSVSLNHLLLLKYLWTCKRDCERLAVLHCFLLHLDQKPLWETEIIEWICENLSVCQMFDPIQEFIIASLYRRMSLETIYLDKLLPYTSLPFWNQGIEGVLWSSDITDMPILKIGMFKGFWEKFHSELEYMKHTHHNNPVHRRDWCKQIADVDTQEWRNIIYQRNILENAKKVILNMETWSSDQRIVPLHQEEDYLQKDLMWLMTIKLKDHLSQPLLHVGKNNIDKIVLDYISKQLDLICLILEVVWKKYNLDLYAFSFSRGCSSDYSAFYMMCHISESAIKLGMPLPPGFQTLHLVLGVRCLPLGNLLHYIDAGILQLTEAFAVKLLKELDDNESNEKLKYSIIVRLPERICQNIHRLWDHAISNNCIARKYVEQLLCKLKKRECHLAGQAACSQSALSMNFLPLNYLIKMLSTIEEQALNPFEEDNIDAAFLEEIALKQTTVLLRLHKS

Target ORF information:

RefSeq Version	XM_012960013.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960013.3

ORF Insert Sequence:

ATGGTCCTGG ATTTCTGTGC AAGTTTTTCT CTGCAGAGAG ATGTCATGCC GTGGATCCTG 
GCCCGGGAAG CCTCTGAAAT TTCAAAGAGG AACTCAAGGA CCCTCCGTAT TGTGAATGTT 
GAGCGAAATG GAAGAGTTCT CTTTACCTGG AAGGGAGGCG ATGGTCTTAC CAACATTGGT 
TTGTATGATC CTGATTTAAG ACAAAATGAG ATGCTTTATT CATTTGACAA AGATCTACTT 
ATTATAAGCT GTTCAGTGAA CTGTGAAAAA AGTCTGCTTG CATTAAGTTA CTGTAATTCA 
GTCAAAGAAC ATCAATATGA ACCTCTTAGA ACAGTTTCCA AATACTTGGC ATTACTGATT 
GAAATAAAAC CAGTCAACAA TGTCAGAGTT CTAAAAGCTG TGGACTATGA CATTAGAGTA 
CAGTTCTTAT ATCCAACTGA AGACGTTTGT CCATTTCCTG AAAGTCATCT TTTGTTGGTT 
TCGAAAGAGA AGTATATTGA ACAATTTCAT GTTATACTTA CTGTTGAAGG TGACAGTAGT 
GTGGTTATCA AAAATTCTGG CAAGCTTCCA AGGGAAAGAA TAGCAGAAGA CTTTGTATGG 
GTTCAGTGGG ATATGTTACA TCAGAGACTT TTTTACATTA TTCCTAAGCA TTCCAGTTGC 
GTCTTGCATT GCATCCAGTT TTATCATGAA GATCATTTCA AAATAATTTT TGAAGTTTCT 
CTTGAGATAA TTTTCGTTTA CCAAGCAGTA TGTTTGATCA ATTTGGGCTT TGACCCTTAT 
GAAGTGAAAG AGCAAGAAAG TTCTACATCT CTAAACATAC AGGTGTTTAC AGATAATACA 
GGAGGTCTGT GCTTGTTTTA TCTCCAGCCT TTCAAAGATG CAAAGGAGGT GAAATACATG 
GTGATATTTT TGCACAGAGG TTGTAGTAAG ACCTTTAAAG TTGCATTATC CGCAGAAGAG 
ATGAAACAGC TAAAGAAAAT GTCTTTTATT AATCTTGGTG GTTATGTTGC TGTTTACTTG 
CCTGACCATT TTTTGCATTT GATAAACACC AGGCATCCTG ACTTGATGTG CTACCACTTG 
TTTTTGGCAG ATACTGATGC CAGAATAGGT GAGATATGCT GTGATGGTCC TGTTCAGTCT 
GTTCTAATGT CGTCAGTGAT TGAGTACTGC CAGGGGATTC TCTTCTCAGT CAGCCTAAAC 
CACTTGCTTC TTCTGAAATA CCTTTGGACT TGTAAAAGGG ATTGTGAAAG GCTGGCAGTA 
CTTCATTGTT TTCTTTTGCA CCTGGATCAG AAGCCACTAT GGGAAACAGA GATTATTGAG 
TGGATATGTG AAAACCTCTC AGTATGCCAA ATGTTTGATC CCATACAGGA ATTTATAATT 
GCTTCTTTAT ACAGAAGAAT GAGTTTGGAA ACTATTTATC TAGATAAACT GCTGCCTTAT 
ACTTCACTGC CTTTTTGGAA TCAGGGCATT GAGGGTGTGT TATGGTCATC AGACATCACA 
GATATGCCAA TATTAAAGAT TGGTATGTTT AAAGGATTCT GGGAGAAGTT CCATTCTGAG 
CTGGAATATA TGAAACATAC TCACCACAAT AACCCTGTGC ACCGAAGAGA TTGGTGTAAA 
CAAATTGCTG ATGTGGACAC ACAAGAATGG AGGAATATTA TTTATCAGCG CAACATTTTG 
GAAAATGCAA AAAAAGTTAT TTTAAACATG GAAACATGGA GTTCTGATCA GAGAATTGTG 
CCGCTTCATC AGGAAGAGGA TTATCTACAG AAAGACCTCA TGTGGCTGAT GACGATTAAA 
TTGAAGGATC ACCTGTCACA GCCTTTGCTT CATGTTGGAA AAAATAACAT TGATAAGATT 
GTTCTGGATT ACATTTCAAA GCAGTTAGAC TTGATATGCC TGATACTGGA AGTTGTGTGG 
AAAAAATACA ACTTGGATCT ATATGCATTC TCTTTTTCAA GAGGATGTTC TTCAGACTAT 
TCTGCCTTTT ACATGATGTG CCACATTTCA GAATCTGCAA TCAAACTGGG CATGCCTCTT 
CCACCTGGAT TCCAGACACT ACATTTGGTC CTGGGAGTTC GATGCCTTCC TCTAGGAAAT 
CTTCTGCATT ATATAGATGC TGGAATTCTA CAACTGACTG AAGCTTTTGC TGTCAAACTT 
CTAAAAGAAC TGGATGACAA TGAAAGCAAT GAAAAGTTAA AATACAGCAT TATTGTACGT 
CTTCCTGAGA GAATTTGCCA AAATATTCAT CGCTTATGGG ATCATGCCAT TAGTAATAAC 
TGCATCGCCA GAAAATATGT TGAACAATTA TTGTGCAAGC TTAAAAAAAG GGAGTGTCAC 
CTTGCAGGAC AAGCAGCTTG TAGTCAGTCA GCATTGTCCA TGAATTTCTT GCCACTTAAC 
TACTTAATCA AGATGCTGTC AACAATAGAA GAACAAGCAT TAAATCCCTT TGAAGAAGAT 
AACATAGATG CTGCATTTCT GGAGGAAATC GCCTTAAAAC AGACTACTGT CCTTCTTAGG 
TTACACAAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsap ( XM_031899025.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754885.1 Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X4, mRNA.

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CloneID	OXa54864
Clone ID Related Accession (Same CDS sequence)	XM_031899025.1
Accession Version	XM_031899025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	gamma-secretase-activating protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTGG ATTTCTGTGC AAGTTTTTCT CTGCAGAGAG ATGTCATGCC GTGGATCCTG 
GCCCGGGAAG CCTCTGAAAT TTCAAAGAGG AACTCAAGGA CCCTCCGTAT TGTGAATGTT 
GAGCGAAATG GAAGAGTTCT CTTTACCTGG AAGGGAGGCG ATGGTCTTAC CAACATTGGT 
TTGTATGATC CTGATTTAAG ACAAAATGAG ATGCTTTATT CATTTGACAA AGATCTACTT 
ATTATAAGCT GTTCAGTGAA CTGTGAAAAA AGTCTGCTTG CATTAAGTTA CTGTAATTCA 
GTCAAAGAAC ATCAATATGA ACCTCTTAGA ACAGTTTCCA AATACTTGGC ATTACTGATT 
GAAATAAAAC CAGTCAACAA TGTCAGAGTT CTAAAAGCTG TGGACTATGA CATTAGAGTA 
CAGTTCTTAT ATCCAACTGA AGACGTTTGT CCATTTCCTG AAAGTCATCT TTTGTTGGTT 
TCGAAAGAGA AGTGTGACAG TAGTGTGGTT ATCAAAAATT CTGGCAAGCT TCCAAGGGAA 
AGAATAGCAG AAGACTTTGT ATGGGTTCAG TGGGATATGT TACATCAGAG ACTTTTTTAC 
ATTATTCCTA AGCATTCCAG TTGCGTCTTG CATTGCATCC AGTTTTATCA TGAAGATCAT 
TTCAAAATAA TTTTTGAAGT TTCTCTTGAG ATAATTTTCG TTTACCAAGC AGTATGTTTG 
ATCAATTTGG GCTTTGACCC TTATGAAGTG AAAGAGCAAG AAAGTTCTAC ATCTCTAAAC 
ATACAGGTGT TTACAGATAA TACAGGAGGT CTGTGCTTGT TTTATCTCCA GCCTTTCAAA 
GATGCAAAGG AGGTGAAATA CATGGTGATA TTTTTGCACA GAGGTTGTAG TAAGACCTTT 
AAAGTTGCAT TATCCGCAGA AGAGATGAAA CAGCTAAAGA AAATGTCTTT TATTAATCTT 
GGTGGTTATG TTGCTGTTTA CTTGCCTGAC CATTTTTTGC ATTTGATAAA CACCAGGCAT 
CCTGACTTGA TGTGCTACCA CTTGTTTTTG GCAGATACTG ATGCCAGAAT AGGTGAGATA 
TGCTGTGATG GTCCTGTTCA GTCTGTTCTA ATGTCGTCAG TGATTGAGTA CTGCCAGGGG 
ATTCTCTTCT CAGTCAGCCT AAACCACTTG CTTCTTCTGA AATACCTTTG GACTTGTAAA 
AGGGATTGTG AAAGGCTGGC AGTACTTCAT TGTTTTCTTT TGCACCTGGA TCAGAAGCCA 
CTATGGGAAA CAGAGATTAT TGAGTGGATA TGTGAAAACC TCTCAGTATG CCAAATGTTT 
GATCCCATAC AGGAATTTAT AATTGCTTCT TTATACAGAA GAATGAGTTT GGAAACTATT 
TATCTAGATA AACTGCTGCC TTATACTTCA CTGCCTTTTT GGAATCAGGG CATTGAGGGT 
GTGTTATGGT CATCAGACAT CACAGATATG CCAATATTAA AGATTGGTAT GTTTAAAGGA 
TTCTGGGAGA AGTTCCATTC TGAGCTGGAA TATATGAAAC ATACTCACCA CAATAACCCT 
GTGCACCGAA GAGATTGGTG TAAACAAATT GCTGATGTGG ACACACAAGA ATGGAGGAAT 
ATTATTTATC AGCGCAACAT TTTGGAAAAT GCAAAAAAAG TTATTTTAAA CATGGAAACA 
TGGAGTTCTG ATCAGAGAAT TGTGCCGCTT CATCAGGAAG AGGATTATCT ACAGAAAGAC 
CTCATGTGGC TGATGACGAT TAAATTGAAG GATCACCTGT CACAGCCTTT GCTTCATGTT 
GGAAAAAATA ACATTGATAA GATTGTTCTG GATTACATTT CAAAGCAGTT AGACTTGATA 
TGCCTGATAC TGGAAGTTGT GTGGAAAAAA TACAACTTGG ATCTATATGC ATTCTCTTTC 
CTATTTATTT GTAGTAGTTC AAGAGGATGT TCTTCAGACT ATTCTGCCTT TTACATGATG 
TGCCACATTT CAGAATCTGC AATCAAACTG GGCATGCCTC TTCCACCTGG ATTCCAGACA 
CTACATTTGG TCCTGGGAGT TCGATGCCTT CCTCTAGGAA ATCTTCTGCA TTATATAGAT 
GCTGGAATTC TACAACTGAC TGAAGCTTTT GCTGTCAAAC TTCTAAAAGA ACTGGATGAC 
AATGAAAGCA ATGAAAAGTT AAAATACAGC ATTATTGTAC GTCTTCCTGA GAGAATTTGC 
CAAAATATTC ATCGCTTATG GGATCATGCC ATTAGTAATA ACTGCATCGC CAGAAAATAT 
GTTGAACAAT TATTGTGCAA GCTTAAAAAA AGGGAGTGTC ACCTTGCAGG ACAAGCAGCT 
TGTAGTCAGT CAGCATTGTC CATGAATTTC TTGCCACTTA ACTACTTAAT CAAGATGCTG 
TCAACAATAG AAGAACAAGC ATTAAATCCC TTTGAAGAAG ATAACATAGA TGCTGCATTT 
CTGGAGGAAA TCGCCTTAAA ACAGACTACT GTCCTTCTTA GGTTACACAA AAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754885.1
CDS	497..3013
Translation	MVLDFCASFSLQRDVMPWILAREASEISKRNSRTLRIVNVERNGRVLFTWKGGDGLTNIGLYDPDLRQNEMLYSFDKDLLIISCSVNCEKSLLALSYCNSVKEHQYEPLRTVSKYLALLIEIKPVNNVRVLKAVDYDIRVQFLYPTEDVCPFPESHLLLVSKEKCDSSVVIKNSGKLPRERIAEDFVWVQWDMLHQRLFYIIPKHSSCVLHCIQFYHEDHFKIIFEVSLEIIFVYQAVCLINLGFDPYEVKEQESSTSLNIQVFTDNTGGLCLFYLQPFKDAKEVKYMVIFLHRGCSKTFKVALSAEEMKQLKKMSFINLGGYVAVYLPDHFLHLINTRHPDLMCYHLFLADTDARIGEICCDGPVQSVLMSSVIEYCQGILFSVSLNHLLLLKYLWTCKRDCERLAVLHCFLLHLDQKPLWETEIIEWICENLSVCQMFDPIQEFIIASLYRRMSLETIYLDKLLPYTSLPFWNQGIEGVLWSSDITDMPILKIGMFKGFWEKFHSELEYMKHTHHNNPVHRRDWCKQIADVDTQEWRNIIYQRNILENAKKVILNMETWSSDQRIVPLHQEEDYLQKDLMWLMTIKLKDHLSQPLLHVGKNNIDKIVLDYISKQLDLICLILEVVWKKYNLDLYAFSFLFICSSSRGCSSDYSAFYMMCHISESAIKLGMPLPPGFQTLHLVLGVRCLPLGNLLHYIDAGILQLTEAFAVKLLKELDDNESNEKLKYSIIVRLPERICQNIHRLWDHAISNNCIARKYVEQLLCKLKKRECHLAGQAACSQSALSMNFLPLNYLIKMLSTIEEQALNPFEEDNIDAAFLEEIALKQTTVLLRLHKS

Target ORF information:

RefSeq Version	XM_031899025.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis gamma-secretase activating protein (gsap), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899025.1

ORF Insert Sequence:

ATGGTCCTGG ATTTCTGTGC AAGTTTTTCT CTGCAGAGAG ATGTCATGCC GTGGATCCTG 
GCCCGGGAAG CCTCTGAAAT TTCAAAGAGG AACTCAAGGA CCCTCCGTAT TGTGAATGTT 
GAGCGAAATG GAAGAGTTCT CTTTACCTGG AAGGGAGGCG ATGGTCTTAC CAACATTGGT 
TTGTATGATC CTGATTTAAG ACAAAATGAG ATGCTTTATT CATTTGACAA AGATCTACTT 
ATTATAAGCT GTTCAGTGAA CTGTGAAAAA AGTCTGCTTG CATTAAGTTA CTGTAATTCA 
GTCAAAGAAC ATCAATATGA ACCTCTTAGA ACAGTTTCCA AATACTTGGC ATTACTGATT 
GAAATAAAAC CAGTCAACAA TGTCAGAGTT CTAAAAGCTG TGGACTATGA CATTAGAGTA 
CAGTTCTTAT ATCCAACTGA AGACGTTTGT CCATTTCCTG AAAGTCATCT TTTGTTGGTT 
TCGAAAGAGA AGTGTGACAG TAGTGTGGTT ATCAAAAATT CTGGCAAGCT TCCAAGGGAA 
AGAATAGCAG AAGACTTTGT ATGGGTTCAG TGGGATATGT TACATCAGAG ACTTTTTTAC 
ATTATTCCTA AGCATTCCAG TTGCGTCTTG CATTGCATCC AGTTTTATCA TGAAGATCAT 
TTCAAAATAA TTTTTGAAGT TTCTCTTGAG ATAATTTTCG TTTACCAAGC AGTATGTTTG 
ATCAATTTGG GCTTTGACCC TTATGAAGTG AAAGAGCAAG AAAGTTCTAC ATCTCTAAAC 
ATACAGGTGT TTACAGATAA TACAGGAGGT CTGTGCTTGT TTTATCTCCA GCCTTTCAAA 
GATGCAAAGG AGGTGAAATA CATGGTGATA TTTTTGCACA GAGGTTGTAG TAAGACCTTT 
AAAGTTGCAT TATCCGCAGA AGAGATGAAA CAGCTAAAGA AAATGTCTTT TATTAATCTT 
GGTGGTTATG TTGCTGTTTA CTTGCCTGAC CATTTTTTGC ATTTGATAAA CACCAGGCAT 
CCTGACTTGA TGTGCTACCA CTTGTTTTTG GCAGATACTG ATGCCAGAAT AGGTGAGATA 
TGCTGTGATG GTCCTGTTCA GTCTGTTCTA ATGTCGTCAG TGATTGAGTA CTGCCAGGGG 
ATTCTCTTCT CAGTCAGCCT AAACCACTTG CTTCTTCTGA AATACCTTTG GACTTGTAAA 
AGGGATTGTG AAAGGCTGGC AGTACTTCAT TGTTTTCTTT TGCACCTGGA TCAGAAGCCA 
CTATGGGAAA CAGAGATTAT TGAGTGGATA TGTGAAAACC TCTCAGTATG CCAAATGTTT 
GATCCCATAC AGGAATTTAT AATTGCTTCT TTATACAGAA GAATGAGTTT GGAAACTATT 
TATCTAGATA AACTGCTGCC TTATACTTCA CTGCCTTTTT GGAATCAGGG CATTGAGGGT 
GTGTTATGGT CATCAGACAT CACAGATATG CCAATATTAA AGATTGGTAT GTTTAAAGGA 
TTCTGGGAGA AGTTCCATTC TGAGCTGGAA TATATGAAAC ATACTCACCA CAATAACCCT 
GTGCACCGAA GAGATTGGTG TAAACAAATT GCTGATGTGG ACACACAAGA ATGGAGGAAT 
ATTATTTATC AGCGCAACAT TTTGGAAAAT GCAAAAAAAG TTATTTTAAA CATGGAAACA 
TGGAGTTCTG ATCAGAGAAT TGTGCCGCTT CATCAGGAAG AGGATTATCT ACAGAAAGAC 
CTCATGTGGC TGATGACGAT TAAATTGAAG GATCACCTGT CACAGCCTTT GCTTCATGTT 
GGAAAAAATA ACATTGATAA GATTGTTCTG GATTACATTT CAAAGCAGTT AGACTTGATA 
TGCCTGATAC TGGAAGTTGT GTGGAAAAAA TACAACTTGG ATCTATATGC ATTCTCTTTC 
CTATTTATTT GTAGTAGTTC AAGAGGATGT TCTTCAGACT ATTCTGCCTT TTACATGATG 
TGCCACATTT CAGAATCTGC AATCAAACTG GGCATGCCTC TTCCACCTGG ATTCCAGACA 
CTACATTTGG TCCTGGGAGT TCGATGCCTT CCTCTAGGAA ATCTTCTGCA TTATATAGAT 
GCTGGAATTC TACAACTGAC TGAAGCTTTT GCTGTCAAAC TTCTAAAAGA ACTGGATGAC 
AATGAAAGCA ATGAAAAGTT AAAATACAGC ATTATTGTAC GTCTTCCTGA GAGAATTTGC 
CAAAATATTC ATCGCTTATG GGATCATGCC ATTAGTAATA ACTGCATCGC CAGAAAATAT 
GTTGAACAAT TATTGTGCAA GCTTAAAAAA AGGGAGTGTC ACCTTGCAGG ACAAGCAGCT 
TGTAGTCAGT CAGCATTGTC CATGAATTTC TTGCCACTTA ACTACTTAAT CAAGATGCTG 
TCAACAATAG AAGAACAAGC ATTAAATCCC TTTGAAGAAG ATAACATAGA TGCTGCATTT 
CTGGAGGAAA TCGCCTTAAA ACAGACTACT GTCCTTCTTA GGTTACACAA AAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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gsap cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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