LOC100493526 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100493526
Entrez Gene ID	100493526
Full Name	trichohyalin-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012968620.2	XP_012824074.1	trichohyalin-like isoform X2
XM_002931720.5	XP_002931766.2	trichohyalin isoform X2
XM_018090643.1	XP_017946132.1	trichohyalin-like isoform X1
XM_002931720.4	XP_002931766.2	trichohyalin-like isoform X1
XM_004910493.3	XP_004910550.1	trichohyalin-like isoform X3
XM_031892915.1	XP_031748775.1	trichohyalin isoform X3
XM_031892907.1	XP_031748767.1	trichohyalin isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100493526	XP_004910550.1
Danio rerio (zebrafish)	si:ch211-102c2.8	XP_001341898.4

Gene Ontology

The following LOC100493526 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100493526 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03497	XM_002931720.5 Latest version!	Xenopus tropicalis trichohyalin (LOC100493526), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa49040	XM_031892907.1 Latest version!	Xenopus tropicalis trichohyalin (LOC100493526), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa49041	XM_031892915.1 Latest version!	Xenopus tropicalis trichohyalin (LOC100493526), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa03498	XM_004910493.3 Latest version!	Xenopus tropicalis trichohyalin-like (LOC100493526), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa03497	XM_018090643.1 Latest version!	Xenopus tropicalis trichohyalin-like (LOC100493526), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa35700	XM_012968620.2 Latest version!	Xenopus tropicalis trichohyalin-like (LOC100493526), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OXa03497	XM_002931720.4	Xenopus tropicalis trichohyalin-like (LOC100493526), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100493526 ( XM_002931720.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931766.2 Xenopus tropicalis trichohyalin (LOC100493526), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa03497
Clone ID Related Accession (Same CDS sequence)	XM_002931720.5 , XM_018090643.1 , XM_002931720.4
Accession Version	XM_002931720.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trichohyalin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931720.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGATCTG 
GAACAGTCTC ATAGAATTGT TTCTCAGGAA CCCAGACATG AACATTCCAT TGATTCCAGT 
AAAGAAACAA AAAAGTGTAA GAAAGAGGAG TACAAGTGGA GGCTTTCTCA CCTCTTAGGC 
TCTGAGCAAA GCGATGCCCA GGAATACCAC AGTGACAATT CTACAGAAAG TGTGTGCACT 
GAGGACTTTG TAATAAAGTT CAAAAAAGGC ATGATTGACC CAGTAGTTAG TGGTGGTAAT 
GATGATCACA TCATGAGCAG TTCAGAAAAG AGCTATGGAG ATCATAAAGA AACTTTGGGA 
GACATACTTG AGAAGATGGG TCCCCTAATG GAAGACACAG GAGATCCTGT GCCCCTAAAC 
ACAGCAAGAA GAGAAAATTT CTTGCAAAAA TTTAAAGAAG AGTTACAAGT TGCACTATCA 
TTTAGCGATT CAGACCAAGC TGAAGAATGT GCCAGGAACA GGAGAGACAG TTTGGAATCT 
CTGGGAGGAA GAATATCAAG ACTTAGCCAT GCAAAAATTT CAGAGCCTTC AAGCCTGCTA 
AGCTTTGGGA AAGGCAGTTC AGTAGCAAGT TATTTGCCAT CCAAAAGTGC AGGTGATAGC 
CAGAAAGGTT TGGGTTCTAA GGATGATTAC TTAATTACCA CTTATGCTAT TTATCCCAAC 
ACCTTGGAAA ATGCAAAAGT CAGTATGAAG AATCTGCAGG AAACAAACCA GGGTTTAAAG 
GTAGTGGCAA CGGGAGTAAA AAAAACTGGG AAAGATGGTC TAGGGGCCCA AGTGTCAGAG 
TCCCATTCTT CGGACACAGG ATCAGGTCCT ACTAAAATGC ACAATGATCA AAGTTCACTA 
GAATATTATA AAGAAGAAGG AAAAACAGAA AATACCAGAA GTCTATTGCA AACTAAAGGT 
CAAGTATCAG AGTCAGAAAT ACCAAAAGCA AGCCAAATGG AGGAACTGGA AGGCTCACAA 
AAAGGTTGCT ATTTGAAATA CTTAATATAC AATAAAAAGA ATGGTGGTGT AAGTCAAGAA 
TTTGGCAATA AGGTTCTTAT AGATGATGCT GCAGGAACAC TGTTATCCAG AGATTCGACA 
GAACATTCTG AAAGTGATAA AATGCTTGCA GAGCAAGAAA GAGAAACATA TTATGCAAAA 
ACGCAGGCAT CATGGGAATC AAACTTCAAA CCTGAATTAA GTATCAAAGA CAAAGTGGAT 
AGCCAGACTA ACATAGACGT CTCCTTAACA GATGACAAGA TTGGCATTTC ACAGTATAAA 
CATGTGGCTG GTATCCCGTT GAAGAGTTTT GATGCAGTGA CAGTCGACAG TGATCTGGAC 
ACGGTGACAA CAGAACGAGT ACGGAATCAC ATCCGAAAGG CCATGGGAAC CAAGAAAGAT 
CCTGCACACA ACACAAAAAG ATTGAACCGG CATCGCAATG AGCTCTTAAG ATACTCCAGT 
AACGTGTCCA CAGATGATGA GGAAGAGGAG GAATATCAGA AGTCCTTCAC GGAATGGAGG 
AGCAGAAGTC TCAAGAGATG GCACTCAGAC CCACACAAAG CAGCTGAAAG TACCTGGGAA 
CACAGTGTCT CCAGCCTTCC ACCAGATTTA ATGAATTCCC CTGAAATTGG GCAGCACCAC 
AGACTTCTAG AAGATCTTGC AAGAGAGAAA GAGCGTCATG AAGAATATGT TGATAAACTT 
CGCAGGGACA TAGCTCTTGA AGAAGAGAGA TTAGCTCAAA GGAAATCTCA GCACCGGGAA 
GTTGATGAGT CCTTTAATGA TATTCTACAG CAAAAGAAGG AAGCTTACCT AGAGTTGGAG 
TCCCTGCAAG ATTTACTAGA TCAGACTCAA AAGGAGGTTG TGAAAATGGA GACACGCTTA 
AGGGAGAACC AGATCACAAC TGATGAATTC AGGAATGAGC TGGCAGTACT GGAATACAAG 
CGCAGTGAAT GCCTAAAAAA TCTTCAGGAT CTAGAACTGG AACTGGAAAT GACAAAGCAG 
CAGTGCTCAA GTGCCCAGAG CGGCCAGATA GCCAGTCTCC AGTATGAGAT CTCTTCACTG 
ACAGGTGAAC GAGATGAGCT AAAGGCCCGT TTACGGCACC TGGAGGGCAG CCTTTCATTT 
TTGGAACGTC AGGAACTGGA ACGTCAGCTC ACTTCGGCAA AGTCTGAACT CTTTTCAGAA 
CAACGGGCAG CCAGGGCCAG GACTGATAAA CTTCAAGAGA ATTTAGAAGA GTCTCAGTGC 
AAGTTGGAGG ACAAGACTGC AGAATGTGCA GAGATGCAGG AGAAAACCAA GCAGCTTAAA 
TCTCAGCTCC GTGAACTGGA AAAAAAATAT GAAGCCAAAA TTCAGAGTCA GGCAGAAGAG 
GCCACTGAAG AAAAAGATGC ACTGAATAAA CAAGTCACAG ACTTGAGTTC CCACGCAAGG 
GAGCAGAATA CAAAAGTAAC CTCTCTGGAG AAGATACTGT CTGAAAAGGA GCTCGAGCTG 
ATGAAAATGA GAGATATTAT ATCCACCCTA AAAGCCGATA AGGAAGCACA GACGCTGGCC 
ACAGACTGCC TAAAGGAACA ACAAAACAAG CTGGTGATGG AACTGCAGCT TCAGCACCAG 
CACGACAAGG ATATGCAGAT CACAAAGCTT AAAGAGGAGT TGGAATGCCA GAAACTGAAG 
GACATCCAAC AACTTGCAGA AAATATGGAA CAGGCTAAAA TGAAAGCACT ACAGGAACAA 
GCAGAAGCTC TCAGAAAGGA GGCCAATAAA GTTGTAAAGT CCCTGGAGGA AAAAGACAAA 
GAAATTGCTA AACTAAAAGA GGCCCTAAAA TCCCAGAAGG ATTCTATGAA AAAGCTTGCT 
GTAGAGCTGA AGCAGGAAGC AAGAGAGATG GTGCACAATA CACTGCTGCG AGAGCAGAAG 
AAGTGGGAAA AAGAAAAGAA GGATGCACTA CATATACAGA AACACACGTT AGAGGAGGAG 
AGGCTTCGAG CCATGGCGGA TCTGCGTGAA GCTCTAGAGA GGGAGCGTAG AACAAACATG 
ATGTTAGAGA AGAAATCTGT TGACCTCCAG AATATTATTC AGGAACATGA GGTTCAGAAT 
CGCTCACTCC AGCGGGAGAA ACAAGATGCC TTAGATGAAC TCCGTGCTAT TCTAAAAGAC 
GAAAATCAAG AAGACATGAA AAGACTACAG CAGGAACTAG CTAAAGAGCG AGAGCGAGAT 
ATGGAGCGCC TAAAGCTCAG GCTGCATGAG TTGGAGGAAG AGCATCATGT TCTACGAGCT 
GAAAAGAATG AGGCCATCTT CCGTGAAAGA GAAGCCCTAG CCCAGGCTGA ACGTGCTGAG 
AAAACACTGG CTAGAGAGAT TAGTTCTGTA TCAGAGCGTA TCCAGAATTC CATGGGCCGG 
ACCAGAATAC ATAGTCCTTC ACACAACAGG CATGGCAGTC CCTCTCGCCT CTCTACCAAC 
CAGGCACTGC AGATGCTTCA TGGAGTATGT GATGAAACCA ACCAGCTTGT ACATGAATTA 
CAGCAGGAAG TAGAAGCTCA GAAGCGCACA GTTCTTCATG TGCAGAGAGA AAAGGAGCGA 
GAATTACAGC AACAGAAAGA GCAGTTGCAA TTAGACAAGG AGAAGGCGCT TGAATTAATG 
AAGGAAAGGC TTATACAGGA GCACATTGAA GAAATCACCA ACCTGCAAAG AGACCAGCTT 
AGGGATTCTA GTAGTGCGGA GAGTCAATCT TTGCGGCAGC AACTGCGGGA GAAGGATAAT 
GAGCTGCGCG CTATACAAAG AAATATGGGC AAATGGAAAG ATGAAACTGC TGCAAAGCTT 
GCTCGCAAAT TTGAAGAAGA GCTAAACGCA GAATTAGAAA AAAGTCTGTC CAGGAGTAAG 
GCCTCAGAGA GTCACCGATA CACAGAAAGA GCTGGGTCAG GAATGCGAAG GCTTTCAGTG 
CAAGAAGGAA GAGAAAATTC ACACCTCCGC TCATCCTCCA CTCCATCTTT GAATGTGGTA 
GGTGCACAGC ATGACTTTGG TGCGCTAAAG ATCCTACGGA ATCTGCAGGG CAGAGTCAGA 
GAGCTGCGGA CAGATAACAG CTTGTACAAT GGGGGGAGCA TGGAAGATCT AAGTTTACTG 
AGGACTGATC TAGGAAGTCC CTACAAACAA AAGAGACCAT TAATGACTGA AAGATCAATG 
GCACACCTTA AAGGAACCAT TAGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931766.2
CDS	198..4607
Translation	MTGMAAGKGTDFDDSAEEDTQLKLQMLLAPSQKSEDKQKDHCDYLLDALDEQLDQLQPRSSFKKVEDNRPKKLENSSADLEQSHRIVSQEPRHEHSIDSSKETKKCKKEEYKWRLSHLLGSEQSDAQEYHSDNSTESVCTEDFVIKFKKGMIDPVVSGGNDDHIMSSSEKSYGDHKETLGDILEKMGPLMEDTGDPVPLNTARRENFLQKFKEELQVALSFSDSDQAEECARNRRDSLESLGGRISRLSHAKISEPSSLLSFGKGSSVASYLPSKSAGDSQKGLGSKDDYLITTYAIYPNTLENAKVSMKNLQETNQGLKVVATGVKKTGKDGLGAQVSESHSSDTGSGPTKMHNDQSSLEYYKEEGKTENTRSLLQTKGQVSESEIPKASQMEELEGSQKGCYLKYLIYNKKNGGVSQEFGNKVLIDDAAGTLLSRDSTEHSESDKMLAEQERETYYAKTQASWESNFKPELSIKDKVDSQTNIDVSLTDDKIGISQYKHVAGIPLKSFDAVTVDSDLDTVTTERVRNHIRKAMGTKKDPAHNTKRLNRHRNELLRYSSNVSTDDEEEEEYQKSFTEWRSRSLKRWHSDPHKAAESTWEHSVSSLPPDLMNSPEIGQHHRLLEDLAREKERHEEYVDKLRRDIALEEERLAQRKSQHREVDESFNDILQQKKEAYLELESLQDLLDQTQKEVVKMETRLRENQITTDEFRNELAVLEYKRSECLKNLQDLELELEMTKQQCSSAQSGQIASLQYEISSLTGERDELKARLRHLEGSLSFLERQELERQLTSAKSELFSEQRAARARTDKLQENLEESQCKLEDKTAECAEMQEKTKQLKSQLRELEKKYEAKIQSQAEEATEEKDALNKQVTDLSSHAREQNTKVTSLEKILSEKELELMKMRDIISTLKADKEAQTLATDCLKEQQNKLVMELQLQHQHDKDMQITKLKEELECQKLKDIQQLAENMEQAKMKALQEQAEALRKEANKVVKSLEEKDKEIAKLKEALKSQKDSMKKLAVELKQEAREMVHNTLLREQKKWEKEKKDALHIQKHTLEEERLRAMADLREALERERRTNMMLEKKSVDLQNIIQEHEVQNRSLQREKQDALDELRAILKDENQEDMKRLQQELAKERERDMERLKLRLHELEEEHHVLRAEKNEAIFREREALAQAERAEKTLAREISSVSERIQNSMGRTRIHSPSHNRHGSPSRLSTNQALQMLHGVCDETNQLVHELQQEVEAQKRTVLHVQREKERELQQQKEQLQLDKEKALELMKERLIQEHIEEITNLQRDQLRDSSSAESQSLRQQLREKDNELRAIQRNMGKWKDETAAKLARKFEEELNAELEKSLSRSKASESHRYTERAGSGMRRLSVQEGRENSHLRSSSTPSLNVVGAQHDFGALKILRNLQGRVRELRTDNSLYNGGSMEDLSLLRTDLGSPYKQKRPLMTERSMAHLKGTIRK

Target ORF information:

RefSeq Version	XM_002931720.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trichohyalin (LOC100493526), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931720.5

ORF Insert Sequence:

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGATCTG 
GAACAGTCTC ATAGAATTGT TTCTCAGGAA CCCAGACATG AACATTCCAT TGATTCCAGT 
AAAGAAACAA AAAAGTGTAA GAAAGAGGAG TACAAGTGGA GGCTTTCTCA CCTCTTAGGC 
TCTGAGCAAA GCGATGCCCA GGAATACCAC AGTGACAATT CTACAGAAAG TGTGTGCACT 
GAGGACTTTG TAATAAAGTT CAAAAAAGGC ATGATTGACC CAGTAGTTAG TGGTGGTAAT 
GATGATCACA TCATGAGCAG TTCAGAAAAG AGCTATGGAG ATCATAAAGA AACTTTGGGA 
GACATACTTG AGAAGATGGG TCCCCTAATG GAAGACACAG GAGATCCTGT GCCCCTAAAC 
ACAGCAAGAA GAGAAAATTT CTTGCAAAAA TTTAAAGAAG AGTTACAAGT TGCACTATCA 
TTTAGCGATT CAGACCAAGC TGAAGAATGT GCCAGGAACA GGAGAGACAG TTTGGAATCT 
CTGGGAGGAA GAATATCAAG ACTTAGCCAT GCAAAAATTT CAGAGCCTTC AAGCCTGCTA 
AGCTTTGGGA AAGGCAGTTC AGTAGCAAGT TATTTGCCAT CCAAAAGTGC AGGTGATAGC 
CAGAAAGGTT TGGGTTCTAA GGATGATTAC TTAATTACCA CTTATGCTAT TTATCCCAAC 
ACCTTGGAAA ATGCAAAAGT CAGTATGAAG AATCTGCAGG AAACAAACCA GGGTTTAAAG 
GTAGTGGCAA CGGGAGTAAA AAAAACTGGG AAAGATGGTC TAGGGGCCCA AGTGTCAGAG 
TCCCATTCTT CGGACACAGG ATCAGGTCCT ACTAAAATGC ACAATGATCA AAGTTCACTA 
GAATATTATA AAGAAGAAGG AAAAACAGAA AATACCAGAA GTCTATTGCA AACTAAAGGT 
CAAGTATCAG AGTCAGAAAT ACCAAAAGCA AGCCAAATGG AGGAACTGGA AGGCTCACAA 
AAAGGTTGCT ATTTGAAATA CTTAATATAC AATAAAAAGA ATGGTGGTGT AAGTCAAGAA 
TTTGGCAATA AGGTTCTTAT AGATGATGCT GCAGGAACAC TGTTATCCAG AGATTCGACA 
GAACATTCTG AAAGTGATAA AATGCTTGCA GAGCAAGAAA GAGAAACATA TTATGCAAAA 
ACGCAGGCAT CATGGGAATC AAACTTCAAA CCTGAATTAA GTATCAAAGA CAAAGTGGAT 
AGCCAGACTA ACATAGACGT CTCCTTAACA GATGACAAGA TTGGCATTTC ACAGTATAAA 
CATGTGGCTG GTATCCCGTT GAAGAGTTTT GATGCAGTGA CAGTCGACAG TGATCTGGAC 
ACGGTGACAA CAGAACGAGT ACGGAATCAC ATCCGAAAGG CCATGGGAAC CAAGAAAGAT 
CCTGCACACA ACACAAAAAG ATTGAACCGG CATCGCAATG AGCTCTTAAG ATACTCCAGT 
AACGTGTCCA CAGATGATGA GGAAGAGGAG GAATATCAGA AGTCCTTCAC GGAATGGAGG 
AGCAGAAGTC TCAAGAGATG GCACTCAGAC CCACACAAAG CAGCTGAAAG TACCTGGGAA 
CACAGTGTCT CCAGCCTTCC ACCAGATTTA ATGAATTCCC CTGAAATTGG GCAGCACCAC 
AGACTTCTAG AAGATCTTGC AAGAGAGAAA GAGCGTCATG AAGAATATGT TGATAAACTT 
CGCAGGGACA TAGCTCTTGA AGAAGAGAGA TTAGCTCAAA GGAAATCTCA GCACCGGGAA 
GTTGATGAGT CCTTTAATGA TATTCTACAG CAAAAGAAGG AAGCTTACCT AGAGTTGGAG 
TCCCTGCAAG ATTTACTAGA TCAGACTCAA AAGGAGGTTG TGAAAATGGA GACACGCTTA 
AGGGAGAACC AGATCACAAC TGATGAATTC AGGAATGAGC TGGCAGTACT GGAATACAAG 
CGCAGTGAAT GCCTAAAAAA TCTTCAGGAT CTAGAACTGG AACTGGAAAT GACAAAGCAG 
CAGTGCTCAA GTGCCCAGAG CGGCCAGATA GCCAGTCTCC AGTATGAGAT CTCTTCACTG 
ACAGGTGAAC GAGATGAGCT AAAGGCCCGT TTACGGCACC TGGAGGGCAG CCTTTCATTT 
TTGGAACGTC AGGAACTGGA ACGTCAGCTC ACTTCGGCAA AGTCTGAACT CTTTTCAGAA 
CAACGGGCAG CCAGGGCCAG GACTGATAAA CTTCAAGAGA ATTTAGAAGA GTCTCAGTGC 
AAGTTGGAGG ACAAGACTGC AGAATGTGCA GAGATGCAGG AGAAAACCAA GCAGCTTAAA 
TCTCAGCTCC GTGAACTGGA AAAAAAATAT GAAGCCAAAA TTCAGAGTCA GGCAGAAGAG 
GCCACTGAAG AAAAAGATGC ACTGAATAAA CAAGTCACAG ACTTGAGTTC CCACGCAAGG 
GAGCAGAATA CAAAAGTAAC CTCTCTGGAG AAGATACTGT CTGAAAAGGA GCTCGAGCTG 
ATGAAAATGA GAGATATTAT ATCCACCCTA AAAGCCGATA AGGAAGCACA GACGCTGGCC 
ACAGACTGCC TAAAGGAACA ACAAAACAAG CTGGTGATGG AACTGCAGCT TCAGCACCAG 
CACGACAAGG ATATGCAGAT CACAAAGCTT AAAGAGGAGT TGGAATGCCA GAAACTGAAG 
GACATCCAAC AACTTGCAGA AAATATGGAA CAGGCTAAAA TGAAAGCACT ACAGGAACAA 
GCAGAAGCTC TCAGAAAGGA GGCCAATAAA GTTGTAAAGT CCCTGGAGGA AAAAGACAAA 
GAAATTGCTA AACTAAAAGA GGCCCTAAAA TCCCAGAAGG ATTCTATGAA AAAGCTTGCT 
GTAGAGCTGA AGCAGGAAGC AAGAGAGATG GTGCACAATA CACTGCTGCG AGAGCAGAAG 
AAGTGGGAAA AAGAAAAGAA GGATGCACTA CATATACAGA AACACACGTT AGAGGAGGAG 
AGGCTTCGAG CCATGGCGGA TCTGCGTGAA GCTCTAGAGA GGGAGCGTAG AACAAACATG 
ATGTTAGAGA AGAAATCTGT TGACCTCCAG AATATTATTC AGGAACATGA GGTTCAGAAT 
CGCTCACTCC AGCGGGAGAA ACAAGATGCC TTAGATGAAC TCCGTGCTAT TCTAAAAGAC 
GAAAATCAAG AAGACATGAA AAGACTACAG CAGGAACTAG CTAAAGAGCG AGAGCGAGAT 
ATGGAGCGCC TAAAGCTCAG GCTGCATGAG TTGGAGGAAG AGCATCATGT TCTACGAGCT 
GAAAAGAATG AGGCCATCTT CCGTGAAAGA GAAGCCCTAG CCCAGGCTGA ACGTGCTGAG 
AAAACACTGG CTAGAGAGAT TAGTTCTGTA TCAGAGCGTA TCCAGAATTC CATGGGCCGG 
ACCAGAATAC ATAGTCCTTC ACACAACAGG CATGGCAGTC CCTCTCGCCT CTCTACCAAC 
CAGGCACTGC AGATGCTTCA TGGAGTATGT GATGAAACCA ACCAGCTTGT ACATGAATTA 
CAGCAGGAAG TAGAAGCTCA GAAGCGCACA GTTCTTCATG TGCAGAGAGA AAAGGAGCGA 
GAATTACAGC AACAGAAAGA GCAGTTGCAA TTAGACAAGG AGAAGGCGCT TGAATTAATG 
AAGGAAAGGC TTATACAGGA GCACATTGAA GAAATCACCA ACCTGCAAAG AGACCAGCTT 
AGGGATTCTA GTAGTGCGGA GAGTCAATCT TTGCGGCAGC AACTGCGGGA GAAGGATAAT 
GAGCTGCGCG CTATACAAAG AAATATGGGC AAATGGAAAG ATGAAACTGC TGCAAAGCTT 
GCTCGCAAAT TTGAAGAAGA GCTAAACGCA GAATTAGAAA AAAGTCTGTC CAGGAGTAAG 
GCCTCAGAGA GTCACCGATA CACAGAAAGA GCTGGGTCAG GAATGCGAAG GCTTTCAGTG 
CAAGAAGGAA GAGAAAATTC ACACCTCCGC TCATCCTCCA CTCCATCTTT GAATGTGGTA 
GGTGCACAGC ATGACTTTGG TGCGCTAAAG ATCCTACGGA ATCTGCAGGG CAGAGTCAGA 
GAGCTGCGGA CAGATAACAG CTTGTACAAT GGGGGGAGCA TGGAAGATCT AAGTTTACTG 
AGGACTGATC TAGGAAGTCC CTACAAACAA AAGAGACCAT TAATGACTGA AAGATCAATG 
GCACACCTTA AAGGAACCAT TAGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493526 ( XM_031892907.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748767.1 Xenopus tropicalis trichohyalin (LOC100493526), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa49040
Clone ID Related Accession (Same CDS sequence)	XM_031892907.1
Accession Version	XM_031892907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trichohyalin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGATCTG 
GAACAGTCTC ATAGAATTGT TTCTCAGGAA CCCAGACATG AACATTCCAT TGATTCCAGT 
AAAGAAACAA AAAAGTGTAA GAAAGAGGAG TACAAGTGGA GGCTTTCTCA CCTCTTAGGC 
TCTGAGCAAA GCGATGCCCA GGAATACCAC AGTGACAATT CTACAGAAAG TGTGTGCACT 
GAGGACTTTG TAATAAAGTT CAAAAAAGGC ATGATTGACC CAGTAGTTAG TGGTGGTAAT 
GATGATCACA TCATGAGCAG TTCAGAAAAG AGCTATGGAG ATCATAAAGA AACTTTGGGA 
GACATACTTG AGAAGATGGG TCCCCTAATG GAAGACACAG GAGATCCTGT GCCCCTAAAC 
ACAGCAAGAA GAGAAAATTT CTTGCAAAAA TTTAAAGAAG AGTTACAAGT TGCACTATCA 
TTTAGCGATT CAGACCAAGC TGAAGAATGT GCCAGGAACA GGAGAGACAG TTTGGAATCT 
CTGGGAGGAA GAATATCAAG ACTTAGCCAT GCAAAAATTT CAGAGCCTTC AAGCCTGCTA 
AGCTTTGGGA AAGGCAGTTC AGTAGCAAGT TATTTGCCAT CCAAAAGTGC AGGTGATAGC 
CAGAAAGGTT TGGGTTCTAA GGATGATTAC TTAATTACCA CTTATGCTAT TTATCCCAAC 
ACCTTGGAAA ATGCAAAAGT CAGTATGAAG AATCTGCAGG AAACAAACCA GGGTTTAAAG 
GTAGTGGCAA CGGGAGTAAA AAAAACTGGG AAAGATGGTC TAGGGGCCCA AGTGTCAGAG 
TCCCATTCTT CGGACACAGG ATCAGGTCCT ACTAAAATGC ACAATGATCA AAGTTCACTA 
GAATATTATA AAGAAGAAGG AAAAACAGAA AATACCAGAA GTCTATTGCA AACTAAAGGT 
CAAGTATCAG AGTCAGAAAT ACCAAAAGCA AGCCAAATGG AGGAACTGGA AGGCTCACAA 
AAAGGTTGCT ATTTGAAATA CTTAATATAC AATAAAAAGA ATGGTGGTGT AAGTCAAGAA 
TTTGGCAATA AGGTTCTTAT AGATGATGCT GCAGGAACAC TGTTATCCAG AGATTCGACA 
GAACATTCTG AAAGTGATAA AATGCTTGCA GAGCAAGAAA GAGAAACATA TTATGCAAAA 
ACGCAGGCAT CATGGGAATC AAACTTCAAA CCTGAATTAA GTATCAAAGA CAAAGTGGAT 
AGCCAGACTA ACATAGACGT CTCCTTAACA GATGACAAGA TTGGCATTTC ACAGTATAAA 
CATGTGGCTG GTATCCCGTT GAAGAGTTTT GATGCAGTGA CAGTCGACAG TGATCTGGAC 
ACGGTGACAA CAGAACGAGT ACGGAATCAC ATCCGAAAGG CCATGGGAAC CAAGAAAGAT 
CCTGCACACA ACACAAAAAG ATTGAACCGG CATCGCAATG AGCTCTTAAG ATACTCCAGT 
AACGTGTCCA CAGATGATGA GGAAGAGGAG GAATATCAGA AGTCCTTCAC GGAATGGAGG 
AGCAGAAGTC TCAAGAGATG GCACTCAGAC CCACACAAAG CAGCTGAAAG TACCTGGGAA 
CACAGTGTCT CCAGCCTTCC ACCAGATTTA ATGAATTCCC CTGAAATTGG GCAGCACCAC 
AGACTTCTAG AAGATCTTGC AAGAGAGAAA GAGCGTCATG AAGAATATGT TGATAAACTT 
CGCAGGGACA TAGCTCTTGA AGAAGAGAGA TTAGCTCAAA GGAAATCTCA GCACCGGGAA 
GTTGATGAGT CCTTTAATGA TATTCTACAG CAAAAGAAGG AAGCTTACCT AGAGTTGGAG 
TCCCTGCAAG ATTTACTAGA TCAGACTCAA AAGGAGGTTG TGAAAATGGA GACACGCTTA 
AGGGAGAACC AGATCACAAC TGATGAATTC AGGAATGAGC TGGCAGTACT GGAATACAAG 
CGCAGTGAAT GCCTAAAAAA TCTTCAGGAT CTAGAACTGG AACTGGAAAT GACAAAGCAG 
CAGTGCTCAA GTGCCCAGAG CGGCCAGATA GCCAGTCTCC AGTATGAGAT CTCTTCACTG 
ACAGGTGAAC GAGATGAGCT AAAGGCCCGT TTACGGCACC TGGAGGGCAG CCTTTCATTT 
TTGGAACGTC AGGAACTGGA ACGTCAGCTC ACTTCGGCAA AGTCTGAACT CTTTTCAGAA 
CAACGGGCAG CCAGGGCCAG GACTGATAAA CTTCAAGAGA ATTTAGAAGA GTCTCAGTGC 
AAGTTGGAGG ACAAGACTGC AGAATGTGCA GAGATGCAGG AGAAAACCAA GCAGCTTAAA 
TCTCAGCTCC GTGAACTGGA AAAAAAATAT GAAGCCAAAA TTCAGAGTCA GGCAGAAGAG 
GCCACTGAAG AAAAAGATGC ACTGAATAAA CAAGTCACAG ACTTGAGTTC CCACGCAAGG 
GAGCAGAATA CAAAAGTAAC CTCTCTGGAG AAGATACTGT CTGAAAAGGA GCTCGAGCTG 
ATGAAAATGA GAGATATTAT ATCCACCCTA AAAGCCGATA AGGAAGCACA GACGCTGGCC 
ACAGACTGCC TAAAGGAACA ACAAAACAAG CTGGTGATGG AACTGCAGCT TCAGCACCAG 
CACGACAAGG ATATGCAGAT CACAAAGCTT AAAGAGGAGT TGGAATGCCA GAAACTGAAG 
GACATCCAAC AACTTGCAGA AAATATGGAA CAGGCTAAAA TGAAAGCACT ACAGGAACAA 
GCAGAAGCTC TCAGAAAGGA GGCCAATAAA GTTGTAAAGT CCCTGGAGGA AAAAGACAAA 
GAAATTGCTA AACTAAAAGA GGCCCTAAAA TCCCAGAAGG ATTCTATGAA AAAGCTTGCT 
GTAGAGCTGA AGCAGGAAGC AAGAGAGATG GTGCACAATA CACTGCTGCG AGAGCAGAAG 
AAGTGGGAAA AAGAAAAGAA GGATGCACTA CATATACAGA AACACACGTT AGAGGAGGAG 
AGGCTTCGAG CCATGGCGGA TCTGCGTGAA GCTCTAGAGA GGGAGCGTAG AACAAACATG 
ATGTTAGAGA AGAAATCTGT TGACCTCCAG AATATTATTC AGGAACATGA GGTTCAGAAT 
CGCTCACTCC AGCGGGAGAA ACAAGATGCC TTAGATGAAC TCCGTGCTAT TCTAAAAGAC 
GAAAATCAAG AAGACATGAA AAGACTACAG CAGGAACTAG CTAAAGAGCG AGAGCGAGAT 
ATGGAGCGCC TAAAGCTCAG GCTGCATGAG TTGGAGGAAG AGCATCATGT TCTACGAGCT 
GAAAAGAATG AGGCCATCTT CCGTGAAAGA GAAGCCCTAG CCCAGGCTGA ACGTGCTGAG 
AAAACACTGG CTAGAGAGAT TAGTTCTGTA TCAGAGCGTA TCCAGAATTC CATGGGCCGG 
ACCAGAATAC ATAGTCCTTC ACACAACAGG CATGGCAGTC CCTCTCGCCT CTCTACCAAC 
CAGGCACTGC AGATGCTTCA TGGAGTATGT GATGAAACCA ACCAGCTTGT ACATGAATTA 
CAGCAGGAAG TAGAAGCTCA GAAGCGCACA GTTCTTCATG TGCAGAGAGA AAAGGAGCGA 
GAATTACAGC AACAGAAAGA GCAGTTGCAA TTAGACAAGG AGAAGGCGCT TGAATTAATG 
AAGGAAAGGC TTATACAGGA GCACATTGAA GAAATCACCA ACCTGCAAAG AGACCAGCTT 
AGGGATTCTA GTAGTGCGGA GAGTCAATCT TTGCGGCAGC AACTGCGGGA GAAGGATAAT 
GAGCTGCGCG CTATACAAAG AAATATGGGC AAATGGAAAG ATGAAACTGC TGCAAAGCTT 
GCTCGCAAAT TTGAAGAAGA GCTAAACGCA GAATTAGAAA AATGCAAAGT GGAGTTTTTA 
AAAGAAAGAA GTCTGTCCAG GAGTAAGGCC TCAGAGAGTC ACCGATACAC AGAAAGAGCT 
GGGTCAGGAA TGCGAAGGCT TTCAGTGCAA GAAGGAAGAG AAAATTCACA CCTCCGCTCA 
TCCTCCACTC CATCTTTGAA TGTGGTAGGT GCACAGCATG ACTTTGGTGC GCTAAAGATC 
CTACGGAATC TGCAGGGCAG AGTCAGAGAG CTGCGGACAG ATAACAGCTT GTACAATGGG 
GGGAGCATGG AAGATCTAAG TTTACTGAGG ACTGATCTAG GAAGTCCCTA CAAACAAAAG 
AGACCATTAA TGACTGAAAG ATCAATGGCA CACCTTAAAG GAACCATTAG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748767.1
CDS	198..4634
Translation	MTGMAAGKGTDFDDSAEEDTQLKLQMLLAPSQKSEDKQKDHCDYLLDALDEQLDQLQPRSSFKKVEDNRPKKLENSSADLEQSHRIVSQEPRHEHSIDSSKETKKCKKEEYKWRLSHLLGSEQSDAQEYHSDNSTESVCTEDFVIKFKKGMIDPVVSGGNDDHIMSSSEKSYGDHKETLGDILEKMGPLMEDTGDPVPLNTARRENFLQKFKEELQVALSFSDSDQAEECARNRRDSLESLGGRISRLSHAKISEPSSLLSFGKGSSVASYLPSKSAGDSQKGLGSKDDYLITTYAIYPNTLENAKVSMKNLQETNQGLKVVATGVKKTGKDGLGAQVSESHSSDTGSGPTKMHNDQSSLEYYKEEGKTENTRSLLQTKGQVSESEIPKASQMEELEGSQKGCYLKYLIYNKKNGGVSQEFGNKVLIDDAAGTLLSRDSTEHSESDKMLAEQERETYYAKTQASWESNFKPELSIKDKVDSQTNIDVSLTDDKIGISQYKHVAGIPLKSFDAVTVDSDLDTVTTERVRNHIRKAMGTKKDPAHNTKRLNRHRNELLRYSSNVSTDDEEEEEYQKSFTEWRSRSLKRWHSDPHKAAESTWEHSVSSLPPDLMNSPEIGQHHRLLEDLAREKERHEEYVDKLRRDIALEEERLAQRKSQHREVDESFNDILQQKKEAYLELESLQDLLDQTQKEVVKMETRLRENQITTDEFRNELAVLEYKRSECLKNLQDLELELEMTKQQCSSAQSGQIASLQYEISSLTGERDELKARLRHLEGSLSFLERQELERQLTSAKSELFSEQRAARARTDKLQENLEESQCKLEDKTAECAEMQEKTKQLKSQLRELEKKYEAKIQSQAEEATEEKDALNKQVTDLSSHAREQNTKVTSLEKILSEKELELMKMRDIISTLKADKEAQTLATDCLKEQQNKLVMELQLQHQHDKDMQITKLKEELECQKLKDIQQLAENMEQAKMKALQEQAEALRKEANKVVKSLEEKDKEIAKLKEALKSQKDSMKKLAVELKQEAREMVHNTLLREQKKWEKEKKDALHIQKHTLEEERLRAMADLREALERERRTNMMLEKKSVDLQNIIQEHEVQNRSLQREKQDALDELRAILKDENQEDMKRLQQELAKERERDMERLKLRLHELEEEHHVLRAEKNEAIFREREALAQAERAEKTLAREISSVSERIQNSMGRTRIHSPSHNRHGSPSRLSTNQALQMLHGVCDETNQLVHELQQEVEAQKRTVLHVQREKERELQQQKEQLQLDKEKALELMKERLIQEHIEEITNLQRDQLRDSSSAESQSLRQQLREKDNELRAIQRNMGKWKDETAAKLARKFEEELNAELEKCKVEFLKERSLSRSKASESHRYTERAGSGMRRLSVQEGRENSHLRSSSTPSLNVVGAQHDFGALKILRNLQGRVRELRTDNSLYNGGSMEDLSLLRTDLGSPYKQKRPLMTERSMAHLKGTIRK

Target ORF information:

RefSeq Version	XM_031892907.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trichohyalin (LOC100493526), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892907.1

ORF Insert Sequence:

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGATCTG 
GAACAGTCTC ATAGAATTGT TTCTCAGGAA CCCAGACATG AACATTCCAT TGATTCCAGT 
AAAGAAACAA AAAAGTGTAA GAAAGAGGAG TACAAGTGGA GGCTTTCTCA CCTCTTAGGC 
TCTGAGCAAA GCGATGCCCA GGAATACCAC AGTGACAATT CTACAGAAAG TGTGTGCACT 
GAGGACTTTG TAATAAAGTT CAAAAAAGGC ATGATTGACC CAGTAGTTAG TGGTGGTAAT 
GATGATCACA TCATGAGCAG TTCAGAAAAG AGCTATGGAG ATCATAAAGA AACTTTGGGA 
GACATACTTG AGAAGATGGG TCCCCTAATG GAAGACACAG GAGATCCTGT GCCCCTAAAC 
ACAGCAAGAA GAGAAAATTT CTTGCAAAAA TTTAAAGAAG AGTTACAAGT TGCACTATCA 
TTTAGCGATT CAGACCAAGC TGAAGAATGT GCCAGGAACA GGAGAGACAG TTTGGAATCT 
CTGGGAGGAA GAATATCAAG ACTTAGCCAT GCAAAAATTT CAGAGCCTTC AAGCCTGCTA 
AGCTTTGGGA AAGGCAGTTC AGTAGCAAGT TATTTGCCAT CCAAAAGTGC AGGTGATAGC 
CAGAAAGGTT TGGGTTCTAA GGATGATTAC TTAATTACCA CTTATGCTAT TTATCCCAAC 
ACCTTGGAAA ATGCAAAAGT CAGTATGAAG AATCTGCAGG AAACAAACCA GGGTTTAAAG 
GTAGTGGCAA CGGGAGTAAA AAAAACTGGG AAAGATGGTC TAGGGGCCCA AGTGTCAGAG 
TCCCATTCTT CGGACACAGG ATCAGGTCCT ACTAAAATGC ACAATGATCA AAGTTCACTA 
GAATATTATA AAGAAGAAGG AAAAACAGAA AATACCAGAA GTCTATTGCA AACTAAAGGT 
CAAGTATCAG AGTCAGAAAT ACCAAAAGCA AGCCAAATGG AGGAACTGGA AGGCTCACAA 
AAAGGTTGCT ATTTGAAATA CTTAATATAC AATAAAAAGA ATGGTGGTGT AAGTCAAGAA 
TTTGGCAATA AGGTTCTTAT AGATGATGCT GCAGGAACAC TGTTATCCAG AGATTCGACA 
GAACATTCTG AAAGTGATAA AATGCTTGCA GAGCAAGAAA GAGAAACATA TTATGCAAAA 
ACGCAGGCAT CATGGGAATC AAACTTCAAA CCTGAATTAA GTATCAAAGA CAAAGTGGAT 
AGCCAGACTA ACATAGACGT CTCCTTAACA GATGACAAGA TTGGCATTTC ACAGTATAAA 
CATGTGGCTG GTATCCCGTT GAAGAGTTTT GATGCAGTGA CAGTCGACAG TGATCTGGAC 
ACGGTGACAA CAGAACGAGT ACGGAATCAC ATCCGAAAGG CCATGGGAAC CAAGAAAGAT 
CCTGCACACA ACACAAAAAG ATTGAACCGG CATCGCAATG AGCTCTTAAG ATACTCCAGT 
AACGTGTCCA CAGATGATGA GGAAGAGGAG GAATATCAGA AGTCCTTCAC GGAATGGAGG 
AGCAGAAGTC TCAAGAGATG GCACTCAGAC CCACACAAAG CAGCTGAAAG TACCTGGGAA 
CACAGTGTCT CCAGCCTTCC ACCAGATTTA ATGAATTCCC CTGAAATTGG GCAGCACCAC 
AGACTTCTAG AAGATCTTGC AAGAGAGAAA GAGCGTCATG AAGAATATGT TGATAAACTT 
CGCAGGGACA TAGCTCTTGA AGAAGAGAGA TTAGCTCAAA GGAAATCTCA GCACCGGGAA 
GTTGATGAGT CCTTTAATGA TATTCTACAG CAAAAGAAGG AAGCTTACCT AGAGTTGGAG 
TCCCTGCAAG ATTTACTAGA TCAGACTCAA AAGGAGGTTG TGAAAATGGA GACACGCTTA 
AGGGAGAACC AGATCACAAC TGATGAATTC AGGAATGAGC TGGCAGTACT GGAATACAAG 
CGCAGTGAAT GCCTAAAAAA TCTTCAGGAT CTAGAACTGG AACTGGAAAT GACAAAGCAG 
CAGTGCTCAA GTGCCCAGAG CGGCCAGATA GCCAGTCTCC AGTATGAGAT CTCTTCACTG 
ACAGGTGAAC GAGATGAGCT AAAGGCCCGT TTACGGCACC TGGAGGGCAG CCTTTCATTT 
TTGGAACGTC AGGAACTGGA ACGTCAGCTC ACTTCGGCAA AGTCTGAACT CTTTTCAGAA 
CAACGGGCAG CCAGGGCCAG GACTGATAAA CTTCAAGAGA ATTTAGAAGA GTCTCAGTGC 
AAGTTGGAGG ACAAGACTGC AGAATGTGCA GAGATGCAGG AGAAAACCAA GCAGCTTAAA 
TCTCAGCTCC GTGAACTGGA AAAAAAATAT GAAGCCAAAA TTCAGAGTCA GGCAGAAGAG 
GCCACTGAAG AAAAAGATGC ACTGAATAAA CAAGTCACAG ACTTGAGTTC CCACGCAAGG 
GAGCAGAATA CAAAAGTAAC CTCTCTGGAG AAGATACTGT CTGAAAAGGA GCTCGAGCTG 
ATGAAAATGA GAGATATTAT ATCCACCCTA AAAGCCGATA AGGAAGCACA GACGCTGGCC 
ACAGACTGCC TAAAGGAACA ACAAAACAAG CTGGTGATGG AACTGCAGCT TCAGCACCAG 
CACGACAAGG ATATGCAGAT CACAAAGCTT AAAGAGGAGT TGGAATGCCA GAAACTGAAG 
GACATCCAAC AACTTGCAGA AAATATGGAA CAGGCTAAAA TGAAAGCACT ACAGGAACAA 
GCAGAAGCTC TCAGAAAGGA GGCCAATAAA GTTGTAAAGT CCCTGGAGGA AAAAGACAAA 
GAAATTGCTA AACTAAAAGA GGCCCTAAAA TCCCAGAAGG ATTCTATGAA AAAGCTTGCT 
GTAGAGCTGA AGCAGGAAGC AAGAGAGATG GTGCACAATA CACTGCTGCG AGAGCAGAAG 
AAGTGGGAAA AAGAAAAGAA GGATGCACTA CATATACAGA AACACACGTT AGAGGAGGAG 
AGGCTTCGAG CCATGGCGGA TCTGCGTGAA GCTCTAGAGA GGGAGCGTAG AACAAACATG 
ATGTTAGAGA AGAAATCTGT TGACCTCCAG AATATTATTC AGGAACATGA GGTTCAGAAT 
CGCTCACTCC AGCGGGAGAA ACAAGATGCC TTAGATGAAC TCCGTGCTAT TCTAAAAGAC 
GAAAATCAAG AAGACATGAA AAGACTACAG CAGGAACTAG CTAAAGAGCG AGAGCGAGAT 
ATGGAGCGCC TAAAGCTCAG GCTGCATGAG TTGGAGGAAG AGCATCATGT TCTACGAGCT 
GAAAAGAATG AGGCCATCTT CCGTGAAAGA GAAGCCCTAG CCCAGGCTGA ACGTGCTGAG 
AAAACACTGG CTAGAGAGAT TAGTTCTGTA TCAGAGCGTA TCCAGAATTC CATGGGCCGG 
ACCAGAATAC ATAGTCCTTC ACACAACAGG CATGGCAGTC CCTCTCGCCT CTCTACCAAC 
CAGGCACTGC AGATGCTTCA TGGAGTATGT GATGAAACCA ACCAGCTTGT ACATGAATTA 
CAGCAGGAAG TAGAAGCTCA GAAGCGCACA GTTCTTCATG TGCAGAGAGA AAAGGAGCGA 
GAATTACAGC AACAGAAAGA GCAGTTGCAA TTAGACAAGG AGAAGGCGCT TGAATTAATG 
AAGGAAAGGC TTATACAGGA GCACATTGAA GAAATCACCA ACCTGCAAAG AGACCAGCTT 
AGGGATTCTA GTAGTGCGGA GAGTCAATCT TTGCGGCAGC AACTGCGGGA GAAGGATAAT 
GAGCTGCGCG CTATACAAAG AAATATGGGC AAATGGAAAG ATGAAACTGC TGCAAAGCTT 
GCTCGCAAAT TTGAAGAAGA GCTAAACGCA GAATTAGAAA AATGCAAAGT GGAGTTTTTA 
AAAGAAAGAA GTCTGTCCAG GAGTAAGGCC TCAGAGAGTC ACCGATACAC AGAAAGAGCT 
GGGTCAGGAA TGCGAAGGCT TTCAGTGCAA GAAGGAAGAG AAAATTCACA CCTCCGCTCA 
TCCTCCACTC CATCTTTGAA TGTGGTAGGT GCACAGCATG ACTTTGGTGC GCTAAAGATC 
CTACGGAATC TGCAGGGCAG AGTCAGAGAG CTGCGGACAG ATAACAGCTT GTACAATGGG 
GGGAGCATGG AAGATCTAAG TTTACTGAGG ACTGATCTAG GAAGTCCCTA CAAACAAAAG 
AGACCATTAA TGACTGAAAG ATCAATGGCA CACCTTAAAG GAACCATTAG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493526 ( XM_031892915.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748775.1 Xenopus tropicalis trichohyalin (LOC100493526), transcript variant X3, mRNA.

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CloneID	OXa49041
Clone ID Related Accession (Same CDS sequence)	XM_031892915.1
Accession Version	XM_031892915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trichohyalin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGGTATC 
AAAGACAAAG TGGATAGCCA GACTAACATA GACGTCTCCT TAACAGATGA CAAGATTGGC 
ATTTCACAGT ATAAACATGT GGCTGGTATC CCGTTGAAGA GTTTTGATGC AGTGACAGTC 
GACAGTGATC TGGACACGGT GACAACAGAA CGAGTACGGA ATCACATCCG AAAGGCCATG 
GGAACCAAGA AAGATCCTGC ACACAACACA AAAAGATTGA ACCGGCATCG CAATGAGCTC 
TTAAGATACT CCAGTAACGT GTCCACAGAT GATGAGGAAG AGGAGGAATA TCAGAAGTCC 
TTCACGGAAT GGAGGAGCAG AAGTCTCAAG AGATGGCACT CAGACCCACA CAAAGCAGCT 
GAAAGTACCT GGGAACACAG TGTCTCCAGC CTTCCACCAG ATTTAATGAA TTCCCCTGAA 
ATTGGGCAGC ACCACAGACT TCTAGAAGAT CTTGCAAGAG AGAAAGAGCG TCATGAAGAA 
TATGTTGATA AACTTCGCAG GGACATAGCT CTTGAAGAAG AGAGATTAGC TCAAAGGAAA 
TCTCAGCACC GGGAAGTTGA TGAGTCCTTT AATGATATTC TACAGCAAAA GAAGGAAGCT 
TACCTAGAGT TGGAGTCCCT GCAAGATTTA CTAGATCAGA CTCAAAAGGA GGTTGTGAAA 
ATGGAGACAC GCTTAAGGGA GAACCAGATC ACAACTGATG AATTCAGGAA TGAGCTGGCA 
GTACTGGAAT ACAAGCGCAG TGAATGCCTA AAAAATCTTC AGGATCTAGA ACTGGAACTG 
GAAATGACAA AGCAGCAGTG CTCAAGTGCC CAGAGCGGCC AGATAGCCAG TCTCCAGTAT 
GAGATCTCTT CACTGACAGG TGAACGAGAT GAGCTAAAGG CCCGTTTACG GCACCTGGAG 
GGCAGCCTTT CATTTTTGGA ACGTCAGGAA CTGGAACGTC AGCTCACTTC GGCAAAGTCT 
GAACTCTTTT CAGAACAACG GGCAGCCAGG GCCAGGACTG ATAAACTTCA AGAGAATTTA 
GAAGAGTCTC AGTGCAAGTT GGAGGACAAG ACTGCAGAAT GTGCAGAGAT GCAGGAGAAA 
ACCAAGCAGC TTAAATCTCA GCTCCGTGAA CTGGAAAAAA AATATGAAGC CAAAATTCAG 
AGTCAGGCAG AAGAGGCCAC TGAAGAAAAA GATGCACTGA ATAAACAAGT CACAGACTTG 
AGTTCCCACG CAAGGGAGCA GAATACAAAA GTAACCTCTC TGGAGAAGAT ACTGTCTGAA 
AAGGAGCTCG AGCTGATGAA AATGAGAGAT ATTATATCCA CCCTAAAAGC CGATAAGGAA 
GCACAGACGC TGGCCACAGA CTGCCTAAAG GAACAACAAA ACAAGCTGGT GATGGAACTG 
CAGCTTCAGC ACCAGCACGA CAAGGATATG CAGATCACAA AGCTTAAAGA GGAGTTGGAA 
TGCCAGAAAC TGAAGGACAT CCAACAACTT GCAGAAAATA TGGAACAGGC TAAAATGAAA 
GCACTACAGG AACAAGCAGA AGCTCTCAGA AAGGAGGCCA ATAAAGTTGT AAAGTCCCTG 
GAGGAAAAAG ACAAAGAAAT TGCTAAACTA AAAGAGGCCC TAAAATCCCA GAAGGATTCT 
ATGAAAAAGC TTGCTGTAGA GCTGAAGCAG GAAGCAAGAG AGATGGTGCA CAATACACTG 
CTGCGAGAGC AGAAGAAGTG GGAAAAAGAA AAGAAGGATG CACTACATAT ACAGAAACAC 
ACGTTAGAGG AGGAGAGGCT TCGAGCCATG GCGGATCTGC GTGAAGCTCT AGAGAGGGAG 
CGTAGAACAA ACATGATGTT AGAGAAGAAA TCTGTTGACC TCCAGAATAT TATTCAGGAA 
CATGAGGTTC AGAATCGCTC ACTCCAGCGG GAGAAACAAG ATGCCTTAGA TGAACTCCGT 
GCTATTCTAA AAGACGAAAA TCAAGAAGAC ATGAAAAGAC TACAGCAGGA ACTAGCTAAA 
GAGCGAGAGC GAGATATGGA GCGCCTAAAG CTCAGGCTGC ATGAGTTGGA GGAAGAGCAT 
CATGTTCTAC GAGCTGAAAA GAATGAGGCC ATCTTCCGTG AAAGAGAAGC CCTAGCCCAG 
GCTGAACGTG CTGAGAAAAC ACTGGCTAGA GAGATTAGTT CTGTATCAGA GCGTATCCAG 
AATTCCATGG GCCGGACCAG AATACATAGT CCTTCACACA ACAGGCATGG CAGTCCCTCT 
CGCCTCTCTA CCAACCAGGC ACTGCAGATG CTTCATGGAG TATGTGATGA AACCAACCAG 
CTTGTACATG AATTACAGCA GGAAGTAGAA GCTCAGAAGC GCACAGTTCT TCATGTGCAG 
AGAGAAAAGG AGCGAGAATT ACAGCAACAG AAAGAGCAGT TGCAATTAGA CAAGGAGAAG 
GCGCTTGAAT TAATGAAGGA AAGGCTTATA CAGGAGCACA TTGAAGAAAT CACCAACCTG 
CAAAGAGACC AGCTTAGGGA TTCTAGTAGT GCGGAGAGTC AATCTTTGCG GCAGCAACTG 
CGGGAGAAGG ATAATGAGCT GCGCGCTATA CAAAGAAATA TGGGCAAATG GAAAGATGAA 
ACTGCTGCAA AGCTTGCTCG CAAATTTGAA GAAGAGCTAA ACGCAGAATT AGAAAAATGC 
AAAGTGGAGT TTTTAAAAGA AAGAAGTCTG TCCAGGAGTA AGGCCTCAGA GAGTCACCGA 
TACACAGAAA GAGCTGGGTC AGGAATGCGA AGGCTTTCAG TGCAAGAAGG AAGAGAAAAT 
TCACACCTCC GCTCATCCTC CACTCCATCT TTGAATGTGG TAGGTGCACA GCATGACTTT 
GGTGCGCTAA AGATCCTACG GAATCTGCAG GGCAGAGTCA GAGAGCTGCG GACAGATAAC 
AGCTTGTACA ATGGGGGGAG CATGGAAGAT CTAAGTTTAC TGAGGACTGA TCTAGGAAGT 
CCCTACAAAC AAAAGAGACC ATTAATGACT GAAAGATCAA TGGCACACCT TAAAGGAACC 
ATTAGGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748775.1
CDS	198..3449
Translation	MTGMAAGKGTDFDDSAEEDTQLKLQMLLAPSQKSEDKQKDHCDYLLDALDEQLDQLQPRSSFKKVEDNRPKKLENSSAGIKDKVDSQTNIDVSLTDDKIGISQYKHVAGIPLKSFDAVTVDSDLDTVTTERVRNHIRKAMGTKKDPAHNTKRLNRHRNELLRYSSNVSTDDEEEEEYQKSFTEWRSRSLKRWHSDPHKAAESTWEHSVSSLPPDLMNSPEIGQHHRLLEDLAREKERHEEYVDKLRRDIALEEERLAQRKSQHREVDESFNDILQQKKEAYLELESLQDLLDQTQKEVVKMETRLRENQITTDEFRNELAVLEYKRSECLKNLQDLELELEMTKQQCSSAQSGQIASLQYEISSLTGERDELKARLRHLEGSLSFLERQELERQLTSAKSELFSEQRAARARTDKLQENLEESQCKLEDKTAECAEMQEKTKQLKSQLRELEKKYEAKIQSQAEEATEEKDALNKQVTDLSSHAREQNTKVTSLEKILSEKELELMKMRDIISTLKADKEAQTLATDCLKEQQNKLVMELQLQHQHDKDMQITKLKEELECQKLKDIQQLAENMEQAKMKALQEQAEALRKEANKVVKSLEEKDKEIAKLKEALKSQKDSMKKLAVELKQEAREMVHNTLLREQKKWEKEKKDALHIQKHTLEEERLRAMADLREALERERRTNMMLEKKSVDLQNIIQEHEVQNRSLQREKQDALDELRAILKDENQEDMKRLQQELAKERERDMERLKLRLHELEEEHHVLRAEKNEAIFREREALAQAERAEKTLAREISSVSERIQNSMGRTRIHSPSHNRHGSPSRLSTNQALQMLHGVCDETNQLVHELQQEVEAQKRTVLHVQREKERELQQQKEQLQLDKEKALELMKERLIQEHIEEITNLQRDQLRDSSSAESQSLRQQLREKDNELRAIQRNMGKWKDETAAKLARKFEEELNAELEKCKVEFLKERSLSRSKASESHRYTERAGSGMRRLSVQEGRENSHLRSSSTPSLNVVGAQHDFGALKILRNLQGRVRELRTDNSLYNGGSMEDLSLLRTDLGSPYKQKRPLMTERSMAHLKGTIRK

Target ORF information:

RefSeq Version	XM_031892915.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trichohyalin (LOC100493526), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892915.1

ORF Insert Sequence:

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGGTATC 
AAAGACAAAG TGGATAGCCA GACTAACATA GACGTCTCCT TAACAGATGA CAAGATTGGC 
ATTTCACAGT ATAAACATGT GGCTGGTATC CCGTTGAAGA GTTTTGATGC AGTGACAGTC 
GACAGTGATC TGGACACGGT GACAACAGAA CGAGTACGGA ATCACATCCG AAAGGCCATG 
GGAACCAAGA AAGATCCTGC ACACAACACA AAAAGATTGA ACCGGCATCG CAATGAGCTC 
TTAAGATACT CCAGTAACGT GTCCACAGAT GATGAGGAAG AGGAGGAATA TCAGAAGTCC 
TTCACGGAAT GGAGGAGCAG AAGTCTCAAG AGATGGCACT CAGACCCACA CAAAGCAGCT 
GAAAGTACCT GGGAACACAG TGTCTCCAGC CTTCCACCAG ATTTAATGAA TTCCCCTGAA 
ATTGGGCAGC ACCACAGACT TCTAGAAGAT CTTGCAAGAG AGAAAGAGCG TCATGAAGAA 
TATGTTGATA AACTTCGCAG GGACATAGCT CTTGAAGAAG AGAGATTAGC TCAAAGGAAA 
TCTCAGCACC GGGAAGTTGA TGAGTCCTTT AATGATATTC TACAGCAAAA GAAGGAAGCT 
TACCTAGAGT TGGAGTCCCT GCAAGATTTA CTAGATCAGA CTCAAAAGGA GGTTGTGAAA 
ATGGAGACAC GCTTAAGGGA GAACCAGATC ACAACTGATG AATTCAGGAA TGAGCTGGCA 
GTACTGGAAT ACAAGCGCAG TGAATGCCTA AAAAATCTTC AGGATCTAGA ACTGGAACTG 
GAAATGACAA AGCAGCAGTG CTCAAGTGCC CAGAGCGGCC AGATAGCCAG TCTCCAGTAT 
GAGATCTCTT CACTGACAGG TGAACGAGAT GAGCTAAAGG CCCGTTTACG GCACCTGGAG 
GGCAGCCTTT CATTTTTGGA ACGTCAGGAA CTGGAACGTC AGCTCACTTC GGCAAAGTCT 
GAACTCTTTT CAGAACAACG GGCAGCCAGG GCCAGGACTG ATAAACTTCA AGAGAATTTA 
GAAGAGTCTC AGTGCAAGTT GGAGGACAAG ACTGCAGAAT GTGCAGAGAT GCAGGAGAAA 
ACCAAGCAGC TTAAATCTCA GCTCCGTGAA CTGGAAAAAA AATATGAAGC CAAAATTCAG 
AGTCAGGCAG AAGAGGCCAC TGAAGAAAAA GATGCACTGA ATAAACAAGT CACAGACTTG 
AGTTCCCACG CAAGGGAGCA GAATACAAAA GTAACCTCTC TGGAGAAGAT ACTGTCTGAA 
AAGGAGCTCG AGCTGATGAA AATGAGAGAT ATTATATCCA CCCTAAAAGC CGATAAGGAA 
GCACAGACGC TGGCCACAGA CTGCCTAAAG GAACAACAAA ACAAGCTGGT GATGGAACTG 
CAGCTTCAGC ACCAGCACGA CAAGGATATG CAGATCACAA AGCTTAAAGA GGAGTTGGAA 
TGCCAGAAAC TGAAGGACAT CCAACAACTT GCAGAAAATA TGGAACAGGC TAAAATGAAA 
GCACTACAGG AACAAGCAGA AGCTCTCAGA AAGGAGGCCA ATAAAGTTGT AAAGTCCCTG 
GAGGAAAAAG ACAAAGAAAT TGCTAAACTA AAAGAGGCCC TAAAATCCCA GAAGGATTCT 
ATGAAAAAGC TTGCTGTAGA GCTGAAGCAG GAAGCAAGAG AGATGGTGCA CAATACACTG 
CTGCGAGAGC AGAAGAAGTG GGAAAAAGAA AAGAAGGATG CACTACATAT ACAGAAACAC 
ACGTTAGAGG AGGAGAGGCT TCGAGCCATG GCGGATCTGC GTGAAGCTCT AGAGAGGGAG 
CGTAGAACAA ACATGATGTT AGAGAAGAAA TCTGTTGACC TCCAGAATAT TATTCAGGAA 
CATGAGGTTC AGAATCGCTC ACTCCAGCGG GAGAAACAAG ATGCCTTAGA TGAACTCCGT 
GCTATTCTAA AAGACGAAAA TCAAGAAGAC ATGAAAAGAC TACAGCAGGA ACTAGCTAAA 
GAGCGAGAGC GAGATATGGA GCGCCTAAAG CTCAGGCTGC ATGAGTTGGA GGAAGAGCAT 
CATGTTCTAC GAGCTGAAAA GAATGAGGCC ATCTTCCGTG AAAGAGAAGC CCTAGCCCAG 
GCTGAACGTG CTGAGAAAAC ACTGGCTAGA GAGATTAGTT CTGTATCAGA GCGTATCCAG 
AATTCCATGG GCCGGACCAG AATACATAGT CCTTCACACA ACAGGCATGG CAGTCCCTCT 
CGCCTCTCTA CCAACCAGGC ACTGCAGATG CTTCATGGAG TATGTGATGA AACCAACCAG 
CTTGTACATG AATTACAGCA GGAAGTAGAA GCTCAGAAGC GCACAGTTCT TCATGTGCAG 
AGAGAAAAGG AGCGAGAATT ACAGCAACAG AAAGAGCAGT TGCAATTAGA CAAGGAGAAG 
GCGCTTGAAT TAATGAAGGA AAGGCTTATA CAGGAGCACA TTGAAGAAAT CACCAACCTG 
CAAAGAGACC AGCTTAGGGA TTCTAGTAGT GCGGAGAGTC AATCTTTGCG GCAGCAACTG 
CGGGAGAAGG ATAATGAGCT GCGCGCTATA CAAAGAAATA TGGGCAAATG GAAAGATGAA 
ACTGCTGCAA AGCTTGCTCG CAAATTTGAA GAAGAGCTAA ACGCAGAATT AGAAAAATGC 
AAAGTGGAGT TTTTAAAAGA AAGAAGTCTG TCCAGGAGTA AGGCCTCAGA GAGTCACCGA 
TACACAGAAA GAGCTGGGTC AGGAATGCGA AGGCTTTCAG TGCAAGAAGG AAGAGAAAAT 
TCACACCTCC GCTCATCCTC CACTCCATCT TTGAATGTGG TAGGTGCACA GCATGACTTT 
GGTGCGCTAA AGATCCTACG GAATCTGCAG GGCAGAGTCA GAGAGCTGCG GACAGATAAC 
AGCTTGTACA ATGGGGGGAG CATGGAAGAT CTAAGTTTAC TGAGGACTGA TCTAGGAAGT 
CCCTACAAAC AAAAGAGACC ATTAATGACT GAAAGATCAA TGGCACACCT TAAAGGAACC 
ATTAGGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493526 ( XM_004910493.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910550.1 Xenopus tropicalis trichohyalin-like (LOC100493526), transcript variant X4, mRNA.

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CloneID	OXa03498
Clone ID Related Accession (Same CDS sequence)	XM_004910493.3
Accession Version	XM_004910493.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trichohyalin-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910493.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGGTATC 
AAAGACAAAG TGGATAGCCA GACTAACATA GACGTCTCCT TAACAGATGA CAAGATTGGC 
ATTTCACAGT ATAAACATGT GGCTGGTATC CCGTTGAAGA GTTTTGATGC AGTGACAGTC 
GACAGTGATC TGGACACGGT GACAACAGAA CGAGTACGGA ATCACATCCG AAAGGCCATG 
GGAACCAAGA AAGATCCTGC ACACAACACA AAAAGATTGA ACCGGCATCG CAATGAGCTC 
TTAAGATACT CCAGTAACGT GTCCACAGAT GATGAGGAAG AGGAGGAATA TCAGAAGTCC 
TTCACGGAAT GGAGGAGCAG AAGTCTCAAG AGATGGCACT CAGACCCACA CAAAGCAGCT 
GAAAGTACCT GGGAACACAG TGTCTCCAGC CTTCCACCAG ATTTAATGAA TTCCCCTGAA 
ATTGGGCAGC ACCACAGACT TCTAGAAGAT CTTGCAAGAG AGAAAGAGCG TCATGAAGAA 
TATGTTGATA AACTTCGCAG GGACATAGCT CTTGAAGAAG AGAGATTAGC TCAAAGGAAA 
TCTCAGCACC GGGAAGTTGA TGAGTCCTTT AATGATATTC TACAGCAAAA GAAGGAAGCT 
TACCTAGAGT TGGAGTCCCT GCAAGATTTA CTAGATCAGA CTCAAAAGGA GGTTGTGAAA 
ATGGAGACAC GCTTAAGGGA GAACCAGATC ACAACTGATG AATTCAGGAA TGAGCTGGCA 
GTACTGGAAT ACAAGCGCAG TGAATGCCTA AAAAATCTTC AGGATCTAGA ACTGGAACTG 
GAAATGACAA AGCAGCAGTG CTCAAGTGCC CAGAGCGGCC AGATAGCCAG TCTCCAGTAT 
GAGATCTCTT CACTGACAGG TGAACGAGAT GAGCTAAAGG CCCGTTTACG GCACCTGGAG 
GGCAGCCTTT CATTTTTGGA ACGTCAGGAA CTGGAACGTC AGCTCACTTC GGCAAAGTCT 
GAACTCTTTT CAGAACAACG GGCAGCCAGG GCCAGGACTG ATAAACTTCA AGAGAATTTA 
GAAGAGTCTC AGTGCAAGTT GGAGGACAAG ACTGCAGAAT GTGCAGAGAT GCAGGAGAAA 
ACCAAGCAGC TTAAATCTCA GCTCCGTGAA CTGGAAAAAA AATATGAAGC CAAAATTCAG 
AGTCAGGCAG AAGAGGCCAC TGAAGAAAAA GATGCACTGA ATAAACAAGT CACAGACTTG 
AGTTCCCACG CAAGGGAGCA GAATACAAAA GTAACCTCTC TGGAGAAGAT ACTGTCTGAA 
AAGGAGCTCG AGCTGATGAA AATGAGAGAT ATTATATCCA CCCTAAAAGC CGATAAGGAA 
GCACAGACGC TGGCCACAGA CTGCCTAAAG GAACAACAAA ACAAGCTGGT GATGGAACTG 
CAGCTTCAGC ACCAGCACGA CAAGGATATG CAGATCACAA AGCTTAAAGA GGAGTTGGAA 
TGCCAGAAAC TGAAGGACAT CCAACAACTT GCAGAAAATA TGGAACAGGC TAAAATGAAA 
GCACTACAGG AACAAGCAGA AGCTCTCAGA AAGGAGGCCA ATAAAGTTGT AAAGTCCCTG 
GAGGAAAAAG ACAAAGAAAT TGCTAAACTA AAAGAGGCCC TAAAATCCCA GAAGGATTCT 
ATGAAAAAGC TTGCTGTAGA GCTGAAGCAG GAAGCAAGAG AGATGGTGCA CAATACACTG 
CTGCGAGAGC AGAAGAAGTG GGAAAAAGAA AAGAAGGATG CACTACATAT ACAGAAACAC 
ACGTTAGAGG AGGAGAGGCT TCGAGCCATG GCGGATCTGC GTGAAGCTCT AGAGAGGGAG 
CGTAGAACAA ACATGATGTT AGAGAAGAAA TCTGTTGACC TCCAGAATAT TATTCAGGAA 
CATGAGGTTC AGAATCGCTC ACTCCAGCGG GAGAAACAAG ATGCCTTAGA TGAACTCCGT 
GCTATTCTAA AAGACGAAAA TCAAGAAGAC ATGAAAAGAC TACAGCAGGA ACTAGCTAAA 
GAGCGAGAGC GAGATATGGA GCGCCTAAAG CTCAGGCTGC ATGAGTTGGA GGAAGAGCAT 
CATGTTCTAC GAGCTGAAAA GAATGAGGCC ATCTTCCGTG AAAGAGAAGC CCTAGCCCAG 
GCTGAACGTG CTGAGAAAAC ACTGGCTAGA GAGATTAGTT CTGTATCAGA GCGTATCCAG 
AATTCCATGG GCCGGACCAG AATACATAGT CCTTCACACA ACAGGCATGG CAGTCCCTCT 
CGCCTCTCTA CCAACCAGGC ACTGCAGATG CTTCATGGAG TATGTGATGA AACCAACCAG 
CTTGTACATG AATTACAGCA GGAAGTAGAA GCTCAGAAGC GCACAGTTCT TCATGTGCAG 
AGAGAAAAGG AGCGAGAATT ACAGCAACAG AAAGAGCAGT TGCAATTAGA CAAGGAGAAG 
GCGCTTGAAT TAATGAAGGA AAGGCTTATA CAGGAGCACA TTGAAGAAAT CACCAACCTG 
CAAAGAGACC AGCTTAGGGA TTCTAGTAGT GCGGAGAGTC AATCTTTGCG GCAGCAACTG 
CGGGAGAAGG ATAATGAGCT GCGCGCTATA CAAAGAAATA TGGGCAAATG GAAAGATGAA 
ACTGCTGCAA AGCTTGCTCG CAAATTTGAA GAAGAGCTAA ACGCAGAATT AGAAAAAAGT 
CTGTCCAGGA GTAAGGCCTC AGAGAGTCAC CGATACACAG AAAGAGCTGG GTCAGGAATG 
CGAAGGCTTT CAGTGCAAGA AGGAAGAGAA AATTCACACC TCCGCTCATC CTCCACTCCA 
TCTTTGAATG TGGTAGGTGC ACAGCATGAC TTTGGTGCGC TAAAGATCCT ACGGAATCTG 
CAGGGCAGAG TCAGAGAGCT GCGGACAGAT AACAGCTTGT ACAATGGGGG GAGCATGGAA 
GATCTAAGTT TACTGAGGAC TGATCTAGGA AGTCCCTACA AACAAAAGAG ACCATTAATG 
ACTGAAAGAT CAATGGCACA CCTTAAAGGA ACCATTAGGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910550.1
CDS	198..3422
Translation	MTGMAAGKGTDFDDSAEEDTQLKLQMLLAPSQKSEDKQKDHCDYLLDALDEQLDQLQPRSSFKKVEDNRPKKLENSSAGIKDKVDSQTNIDVSLTDDKIGISQYKHVAGIPLKSFDAVTVDSDLDTVTTERVRNHIRKAMGTKKDPAHNTKRLNRHRNELLRYSSNVSTDDEEEEEYQKSFTEWRSRSLKRWHSDPHKAAESTWEHSVSSLPPDLMNSPEIGQHHRLLEDLAREKERHEEYVDKLRRDIALEEERLAQRKSQHREVDESFNDILQQKKEAYLELESLQDLLDQTQKEVVKMETRLRENQITTDEFRNELAVLEYKRSECLKNLQDLELELEMTKQQCSSAQSGQIASLQYEISSLTGERDELKARLRHLEGSLSFLERQELERQLTSAKSELFSEQRAARARTDKLQENLEESQCKLEDKTAECAEMQEKTKQLKSQLRELEKKYEAKIQSQAEEATEEKDALNKQVTDLSSHAREQNTKVTSLEKILSEKELELMKMRDIISTLKADKEAQTLATDCLKEQQNKLVMELQLQHQHDKDMQITKLKEELECQKLKDIQQLAENMEQAKMKALQEQAEALRKEANKVVKSLEEKDKEIAKLKEALKSQKDSMKKLAVELKQEAREMVHNTLLREQKKWEKEKKDALHIQKHTLEEERLRAMADLREALERERRTNMMLEKKSVDLQNIIQEHEVQNRSLQREKQDALDELRAILKDENQEDMKRLQQELAKERERDMERLKLRLHELEEEHHVLRAEKNEAIFREREALAQAERAEKTLAREISSVSERIQNSMGRTRIHSPSHNRHGSPSRLSTNQALQMLHGVCDETNQLVHELQQEVEAQKRTVLHVQREKERELQQQKEQLQLDKEKALELMKERLIQEHIEEITNLQRDQLRDSSSAESQSLRQQLREKDNELRAIQRNMGKWKDETAAKLARKFEEELNAELEKSLSRSKASESHRYTERAGSGMRRLSVQEGRENSHLRSSSTPSLNVVGAQHDFGALKILRNLQGRVRELRTDNSLYNGGSMEDLSLLRTDLGSPYKQKRPLMTERSMAHLKGTIRK

Target ORF information:

RefSeq Version	XM_004910493.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trichohyalin-like (LOC100493526), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910493.3

ORF Insert Sequence:

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGGTATC 
AAAGACAAAG TGGATAGCCA GACTAACATA GACGTCTCCT TAACAGATGA CAAGATTGGC 
ATTTCACAGT ATAAACATGT GGCTGGTATC CCGTTGAAGA GTTTTGATGC AGTGACAGTC 
GACAGTGATC TGGACACGGT GACAACAGAA CGAGTACGGA ATCACATCCG AAAGGCCATG 
GGAACCAAGA AAGATCCTGC ACACAACACA AAAAGATTGA ACCGGCATCG CAATGAGCTC 
TTAAGATACT CCAGTAACGT GTCCACAGAT GATGAGGAAG AGGAGGAATA TCAGAAGTCC 
TTCACGGAAT GGAGGAGCAG AAGTCTCAAG AGATGGCACT CAGACCCACA CAAAGCAGCT 
GAAAGTACCT GGGAACACAG TGTCTCCAGC CTTCCACCAG ATTTAATGAA TTCCCCTGAA 
ATTGGGCAGC ACCACAGACT TCTAGAAGAT CTTGCAAGAG AGAAAGAGCG TCATGAAGAA 
TATGTTGATA AACTTCGCAG GGACATAGCT CTTGAAGAAG AGAGATTAGC TCAAAGGAAA 
TCTCAGCACC GGGAAGTTGA TGAGTCCTTT AATGATATTC TACAGCAAAA GAAGGAAGCT 
TACCTAGAGT TGGAGTCCCT GCAAGATTTA CTAGATCAGA CTCAAAAGGA GGTTGTGAAA 
ATGGAGACAC GCTTAAGGGA GAACCAGATC ACAACTGATG AATTCAGGAA TGAGCTGGCA 
GTACTGGAAT ACAAGCGCAG TGAATGCCTA AAAAATCTTC AGGATCTAGA ACTGGAACTG 
GAAATGACAA AGCAGCAGTG CTCAAGTGCC CAGAGCGGCC AGATAGCCAG TCTCCAGTAT 
GAGATCTCTT CACTGACAGG TGAACGAGAT GAGCTAAAGG CCCGTTTACG GCACCTGGAG 
GGCAGCCTTT CATTTTTGGA ACGTCAGGAA CTGGAACGTC AGCTCACTTC GGCAAAGTCT 
GAACTCTTTT CAGAACAACG GGCAGCCAGG GCCAGGACTG ATAAACTTCA AGAGAATTTA 
GAAGAGTCTC AGTGCAAGTT GGAGGACAAG ACTGCAGAAT GTGCAGAGAT GCAGGAGAAA 
ACCAAGCAGC TTAAATCTCA GCTCCGTGAA CTGGAAAAAA AATATGAAGC CAAAATTCAG 
AGTCAGGCAG AAGAGGCCAC TGAAGAAAAA GATGCACTGA ATAAACAAGT CACAGACTTG 
AGTTCCCACG CAAGGGAGCA GAATACAAAA GTAACCTCTC TGGAGAAGAT ACTGTCTGAA 
AAGGAGCTCG AGCTGATGAA AATGAGAGAT ATTATATCCA CCCTAAAAGC CGATAAGGAA 
GCACAGACGC TGGCCACAGA CTGCCTAAAG GAACAACAAA ACAAGCTGGT GATGGAACTG 
CAGCTTCAGC ACCAGCACGA CAAGGATATG CAGATCACAA AGCTTAAAGA GGAGTTGGAA 
TGCCAGAAAC TGAAGGACAT CCAACAACTT GCAGAAAATA TGGAACAGGC TAAAATGAAA 
GCACTACAGG AACAAGCAGA AGCTCTCAGA AAGGAGGCCA ATAAAGTTGT AAAGTCCCTG 
GAGGAAAAAG ACAAAGAAAT TGCTAAACTA AAAGAGGCCC TAAAATCCCA GAAGGATTCT 
ATGAAAAAGC TTGCTGTAGA GCTGAAGCAG GAAGCAAGAG AGATGGTGCA CAATACACTG 
CTGCGAGAGC AGAAGAAGTG GGAAAAAGAA AAGAAGGATG CACTACATAT ACAGAAACAC 
ACGTTAGAGG AGGAGAGGCT TCGAGCCATG GCGGATCTGC GTGAAGCTCT AGAGAGGGAG 
CGTAGAACAA ACATGATGTT AGAGAAGAAA TCTGTTGACC TCCAGAATAT TATTCAGGAA 
CATGAGGTTC AGAATCGCTC ACTCCAGCGG GAGAAACAAG ATGCCTTAGA TGAACTCCGT 
GCTATTCTAA AAGACGAAAA TCAAGAAGAC ATGAAAAGAC TACAGCAGGA ACTAGCTAAA 
GAGCGAGAGC GAGATATGGA GCGCCTAAAG CTCAGGCTGC ATGAGTTGGA GGAAGAGCAT 
CATGTTCTAC GAGCTGAAAA GAATGAGGCC ATCTTCCGTG AAAGAGAAGC CCTAGCCCAG 
GCTGAACGTG CTGAGAAAAC ACTGGCTAGA GAGATTAGTT CTGTATCAGA GCGTATCCAG 
AATTCCATGG GCCGGACCAG AATACATAGT CCTTCACACA ACAGGCATGG CAGTCCCTCT 
CGCCTCTCTA CCAACCAGGC ACTGCAGATG CTTCATGGAG TATGTGATGA AACCAACCAG 
CTTGTACATG AATTACAGCA GGAAGTAGAA GCTCAGAAGC GCACAGTTCT TCATGTGCAG 
AGAGAAAAGG AGCGAGAATT ACAGCAACAG AAAGAGCAGT TGCAATTAGA CAAGGAGAAG 
GCGCTTGAAT TAATGAAGGA AAGGCTTATA CAGGAGCACA TTGAAGAAAT CACCAACCTG 
CAAAGAGACC AGCTTAGGGA TTCTAGTAGT GCGGAGAGTC AATCTTTGCG GCAGCAACTG 
CGGGAGAAGG ATAATGAGCT GCGCGCTATA CAAAGAAATA TGGGCAAATG GAAAGATGAA 
ACTGCTGCAA AGCTTGCTCG CAAATTTGAA GAAGAGCTAA ACGCAGAATT AGAAAAAAGT 
CTGTCCAGGA GTAAGGCCTC AGAGAGTCAC CGATACACAG AAAGAGCTGG GTCAGGAATG 
CGAAGGCTTT CAGTGCAAGA AGGAAGAGAA AATTCACACC TCCGCTCATC CTCCACTCCA 
TCTTTGAATG TGGTAGGTGC ACAGCATGAC TTTGGTGCGC TAAAGATCCT ACGGAATCTG 
CAGGGCAGAG TCAGAGAGCT GCGGACAGAT AACAGCTTGT ACAATGGGGG GAGCATGGAA 
GATCTAAGTT TACTGAGGAC TGATCTAGGA AGTCCCTACA AACAAAAGAG ACCATTAATG 
ACTGAAAGAT CAATGGCACA CCTTAAAGGA ACCATTAGGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493526 ( XM_018090643.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946132.1 Xenopus tropicalis trichohyalin-like (LOC100493526), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa03497
Clone ID Related Accession (Same CDS sequence)	XM_002931720.5 , XM_018090643.1 , XM_002931720.4
Accession Version	XM_018090643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trichohyalin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGATCTG 
GAACAGTCTC ATAGAATTGT TTCTCAGGAA CCCAGACATG AACATTCCAT TGATTCCAGT 
AAAGAAACAA AAAAGTGTAA GAAAGAGGAG TACAAGTGGA GGCTTTCTCA CCTCTTAGGC 
TCTGAGCAAA GCGATGCCCA GGAATACCAC AGTGACAATT CTACAGAAAG TGTGTGCACT 
GAGGACTTTG TAATAAAGTT CAAAAAAGGC ATGATTGACC CAGTAGTTAG TGGTGGTAAT 
GATGATCACA TCATGAGCAG TTCAGAAAAG AGCTATGGAG ATCATAAAGA AACTTTGGGA 
GACATACTTG AGAAGATGGG TCCCCTAATG GAAGACACAG GAGATCCTGT GCCCCTAAAC 
ACAGCAAGAA GAGAAAATTT CTTGCAAAAA TTTAAAGAAG AGTTACAAGT TGCACTATCA 
TTTAGCGATT CAGACCAAGC TGAAGAATGT GCCAGGAACA GGAGAGACAG TTTGGAATCT 
CTGGGAGGAA GAATATCAAG ACTTAGCCAT GCAAAAATTT CAGAGCCTTC AAGCCTGCTA 
AGCTTTGGGA AAGGCAGTTC AGTAGCAAGT TATTTGCCAT CCAAAAGTGC AGGTGATAGC 
CAGAAAGGTT TGGGTTCTAA GGATGATTAC TTAATTACCA CTTATGCTAT TTATCCCAAC 
ACCTTGGAAA ATGCAAAAGT CAGTATGAAG AATCTGCAGG AAACAAACCA GGGTTTAAAG 
GTAGTGGCAA CGGGAGTAAA AAAAACTGGG AAAGATGGTC TAGGGGCCCA AGTGTCAGAG 
TCCCATTCTT CGGACACAGG ATCAGGTCCT ACTAAAATGC ACAATGATCA AAGTTCACTA 
GAATATTATA AAGAAGAAGG AAAAACAGAA AATACCAGAA GTCTATTGCA AACTAAAGGT 
CAAGTATCAG AGTCAGAAAT ACCAAAAGCA AGCCAAATGG AGGAACTGGA AGGCTCACAA 
AAAGGTTGCT ATTTGAAATA CTTAATATAC AATAAAAAGA ATGGTGGTGT AAGTCAAGAA 
TTTGGCAATA AGGTTCTTAT AGATGATGCT GCAGGAACAC TGTTATCCAG AGATTCGACA 
GAACATTCTG AAAGTGATAA AATGCTTGCA GAGCAAGAAA GAGAAACATA TTATGCAAAA 
ACGCAGGCAT CATGGGAATC AAACTTCAAA CCTGAATTAA GTATCAAAGA CAAAGTGGAT 
AGCCAGACTA ACATAGACGT CTCCTTAACA GATGACAAGA TTGGCATTTC ACAGTATAAA 
CATGTGGCTG GTATCCCGTT GAAGAGTTTT GATGCAGTGA CAGTCGACAG TGATCTGGAC 
ACGGTGACAA CAGAACGAGT ACGGAATCAC ATCCGAAAGG CCATGGGAAC CAAGAAAGAT 
CCTGCACACA ACACAAAAAG ATTGAACCGG CATCGCAATG AGCTCTTAAG ATACTCCAGT 
AACGTGTCCA CAGATGATGA GGAAGAGGAG GAATATCAGA AGTCCTTCAC GGAATGGAGG 
AGCAGAAGTC TCAAGAGATG GCACTCAGAC CCACACAAAG CAGCTGAAAG TACCTGGGAA 
CACAGTGTCT CCAGCCTTCC ACCAGATTTA ATGAATTCCC CTGAAATTGG GCAGCACCAC 
AGACTTCTAG AAGATCTTGC AAGAGAGAAA GAGCGTCATG AAGAATATGT TGATAAACTT 
CGCAGGGACA TAGCTCTTGA AGAAGAGAGA TTAGCTCAAA GGAAATCTCA GCACCGGGAA 
GTTGATGAGT CCTTTAATGA TATTCTACAG CAAAAGAAGG AAGCTTACCT AGAGTTGGAG 
TCCCTGCAAG ATTTACTAGA TCAGACTCAA AAGGAGGTTG TGAAAATGGA GACACGCTTA 
AGGGAGAACC AGATCACAAC TGATGAATTC AGGAATGAGC TGGCAGTACT GGAATACAAG 
CGCAGTGAAT GCCTAAAAAA TCTTCAGGAT CTAGAACTGG AACTGGAAAT GACAAAGCAG 
CAGTGCTCAA GTGCCCAGAG CGGCCAGATA GCCAGTCTCC AGTATGAGAT CTCTTCACTG 
ACAGGTGAAC GAGATGAGCT AAAGGCCCGT TTACGGCACC TGGAGGGCAG CCTTTCATTT 
TTGGAACGTC AGGAACTGGA ACGTCAGCTC ACTTCGGCAA AGTCTGAACT CTTTTCAGAA 
CAACGGGCAG CCAGGGCCAG GACTGATAAA CTTCAAGAGA ATTTAGAAGA GTCTCAGTGC 
AAGTTGGAGG ACAAGACTGC AGAATGTGCA GAGATGCAGG AGAAAACCAA GCAGCTTAAA 
TCTCAGCTCC GTGAACTGGA AAAAAAATAT GAAGCCAAAA TTCAGAGTCA GGCAGAAGAG 
GCCACTGAAG AAAAAGATGC ACTGAATAAA CAAGTCACAG ACTTGAGTTC CCACGCAAGG 
GAGCAGAATA CAAAAGTAAC CTCTCTGGAG AAGATACTGT CTGAAAAGGA GCTCGAGCTG 
ATGAAAATGA GAGATATTAT ATCCACCCTA AAAGCCGATA AGGAAGCACA GACGCTGGCC 
ACAGACTGCC TAAAGGAACA ACAAAACAAG CTGGTGATGG AACTGCAGCT TCAGCACCAG 
CACGACAAGG ATATGCAGAT CACAAAGCTT AAAGAGGAGT TGGAATGCCA GAAACTGAAG 
GACATCCAAC AACTTGCAGA AAATATGGAA CAGGCTAAAA TGAAAGCACT ACAGGAACAA 
GCAGAAGCTC TCAGAAAGGA GGCCAATAAA GTTGTAAAGT CCCTGGAGGA AAAAGACAAA 
GAAATTGCTA AACTAAAAGA GGCCCTAAAA TCCCAGAAGG ATTCTATGAA AAAGCTTGCT 
GTAGAGCTGA AGCAGGAAGC AAGAGAGATG GTGCACAATA CACTGCTGCG AGAGCAGAAG 
AAGTGGGAAA AAGAAAAGAA GGATGCACTA CATATACAGA AACACACGTT AGAGGAGGAG 
AGGCTTCGAG CCATGGCGGA TCTGCGTGAA GCTCTAGAGA GGGAGCGTAG AACAAACATG 
ATGTTAGAGA AGAAATCTGT TGACCTCCAG AATATTATTC AGGAACATGA GGTTCAGAAT 
CGCTCACTCC AGCGGGAGAA ACAAGATGCC TTAGATGAAC TCCGTGCTAT TCTAAAAGAC 
GAAAATCAAG AAGACATGAA AAGACTACAG CAGGAACTAG CTAAAGAGCG AGAGCGAGAT 
ATGGAGCGCC TAAAGCTCAG GCTGCATGAG TTGGAGGAAG AGCATCATGT TCTACGAGCT 
GAAAAGAATG AGGCCATCTT CCGTGAAAGA GAAGCCCTAG CCCAGGCTGA ACGTGCTGAG 
AAAACACTGG CTAGAGAGAT TAGTTCTGTA TCAGAGCGTA TCCAGAATTC CATGGGCCGG 
ACCAGAATAC ATAGTCCTTC ACACAACAGG CATGGCAGTC CCTCTCGCCT CTCTACCAAC 
CAGGCACTGC AGATGCTTCA TGGAGTATGT GATGAAACCA ACCAGCTTGT ACATGAATTA 
CAGCAGGAAG TAGAAGCTCA GAAGCGCACA GTTCTTCATG TGCAGAGAGA AAAGGAGCGA 
GAATTACAGC AACAGAAAGA GCAGTTGCAA TTAGACAAGG AGAAGGCGCT TGAATTAATG 
AAGGAAAGGC TTATACAGGA GCACATTGAA GAAATCACCA ACCTGCAAAG AGACCAGCTT 
AGGGATTCTA GTAGTGCGGA GAGTCAATCT TTGCGGCAGC AACTGCGGGA GAAGGATAAT 
GAGCTGCGCG CTATACAAAG AAATATGGGC AAATGGAAAG ATGAAACTGC TGCAAAGCTT 
GCTCGCAAAT TTGAAGAAGA GCTAAACGCA GAATTAGAAA AAAGTCTGTC CAGGAGTAAG 
GCCTCAGAGA GTCACCGATA CACAGAAAGA GCTGGGTCAG GAATGCGAAG GCTTTCAGTG 
CAAGAAGGAA GAGAAAATTC ACACCTCCGC TCATCCTCCA CTCCATCTTT GAATGTGGTA 
GGTGCACAGC ATGACTTTGG TGCGCTAAAG ATCCTACGGA ATCTGCAGGG CAGAGTCAGA 
GAGCTGCGGA CAGATAACAG CTTGTACAAT GGGGGGAGCA TGGAAGATCT AAGTTTACTG 
AGGACTGATC TAGGAAGTCC CTACAAACAA AAGAGACCAT TAATGACTGA AAGATCAATG 
GCACACCTTA AAGGAACCAT TAGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946132.1
CDS	150..4559
Translation	MTGMAAGKGTDFDDSAEEDTQLKLQMLLAPSQKSEDKQKDHCDYLLDALDEQLDQLQPRSSFKKVEDNRPKKLENSSADLEQSHRIVSQEPRHEHSIDSSKETKKCKKEEYKWRLSHLLGSEQSDAQEYHSDNSTESVCTEDFVIKFKKGMIDPVVSGGNDDHIMSSSEKSYGDHKETLGDILEKMGPLMEDTGDPVPLNTARRENFLQKFKEELQVALSFSDSDQAEECARNRRDSLESLGGRISRLSHAKISEPSSLLSFGKGSSVASYLPSKSAGDSQKGLGSKDDYLITTYAIYPNTLENAKVSMKNLQETNQGLKVVATGVKKTGKDGLGAQVSESHSSDTGSGPTKMHNDQSSLEYYKEEGKTENTRSLLQTKGQVSESEIPKASQMEELEGSQKGCYLKYLIYNKKNGGVSQEFGNKVLIDDAAGTLLSRDSTEHSESDKMLAEQERETYYAKTQASWESNFKPELSIKDKVDSQTNIDVSLTDDKIGISQYKHVAGIPLKSFDAVTVDSDLDTVTTERVRNHIRKAMGTKKDPAHNTKRLNRHRNELLRYSSNVSTDDEEEEEYQKSFTEWRSRSLKRWHSDPHKAAESTWEHSVSSLPPDLMNSPEIGQHHRLLEDLAREKERHEEYVDKLRRDIALEEERLAQRKSQHREVDESFNDILQQKKEAYLELESLQDLLDQTQKEVVKMETRLRENQITTDEFRNELAVLEYKRSECLKNLQDLELELEMTKQQCSSAQSGQIASLQYEISSLTGERDELKARLRHLEGSLSFLERQELERQLTSAKSELFSEQRAARARTDKLQENLEESQCKLEDKTAECAEMQEKTKQLKSQLRELEKKYEAKIQSQAEEATEEKDALNKQVTDLSSHAREQNTKVTSLEKILSEKELELMKMRDIISTLKADKEAQTLATDCLKEQQNKLVMELQLQHQHDKDMQITKLKEELECQKLKDIQQLAENMEQAKMKALQEQAEALRKEANKVVKSLEEKDKEIAKLKEALKSQKDSMKKLAVELKQEAREMVHNTLLREQKKWEKEKKDALHIQKHTLEEERLRAMADLREALERERRTNMMLEKKSVDLQNIIQEHEVQNRSLQREKQDALDELRAILKDENQEDMKRLQQELAKERERDMERLKLRLHELEEEHHVLRAEKNEAIFREREALAQAERAEKTLAREISSVSERIQNSMGRTRIHSPSHNRHGSPSRLSTNQALQMLHGVCDETNQLVHELQQEVEAQKRTVLHVQREKERELQQQKEQLQLDKEKALELMKERLIQEHIEEITNLQRDQLRDSSSAESQSLRQQLREKDNELRAIQRNMGKWKDETAAKLARKFEEELNAELEKSLSRSKASESHRYTERAGSGMRRLSVQEGRENSHLRSSSTPSLNVVGAQHDFGALKILRNLQGRVRELRTDNSLYNGGSMEDLSLLRTDLGSPYKQKRPLMTERSMAHLKGTIRK

Target ORF information:

RefSeq Version	XM_018090643.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trichohyalin-like (LOC100493526), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090643.1

ORF Insert Sequence:

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGATCTG 
GAACAGTCTC ATAGAATTGT TTCTCAGGAA CCCAGACATG AACATTCCAT TGATTCCAGT 
AAAGAAACAA AAAAGTGTAA GAAAGAGGAG TACAAGTGGA GGCTTTCTCA CCTCTTAGGC 
TCTGAGCAAA GCGATGCCCA GGAATACCAC AGTGACAATT CTACAGAAAG TGTGTGCACT 
GAGGACTTTG TAATAAAGTT CAAAAAAGGC ATGATTGACC CAGTAGTTAG TGGTGGTAAT 
GATGATCACA TCATGAGCAG TTCAGAAAAG AGCTATGGAG ATCATAAAGA AACTTTGGGA 
GACATACTTG AGAAGATGGG TCCCCTAATG GAAGACACAG GAGATCCTGT GCCCCTAAAC 
ACAGCAAGAA GAGAAAATTT CTTGCAAAAA TTTAAAGAAG AGTTACAAGT TGCACTATCA 
TTTAGCGATT CAGACCAAGC TGAAGAATGT GCCAGGAACA GGAGAGACAG TTTGGAATCT 
CTGGGAGGAA GAATATCAAG ACTTAGCCAT GCAAAAATTT CAGAGCCTTC AAGCCTGCTA 
AGCTTTGGGA AAGGCAGTTC AGTAGCAAGT TATTTGCCAT CCAAAAGTGC AGGTGATAGC 
CAGAAAGGTT TGGGTTCTAA GGATGATTAC TTAATTACCA CTTATGCTAT TTATCCCAAC 
ACCTTGGAAA ATGCAAAAGT CAGTATGAAG AATCTGCAGG AAACAAACCA GGGTTTAAAG 
GTAGTGGCAA CGGGAGTAAA AAAAACTGGG AAAGATGGTC TAGGGGCCCA AGTGTCAGAG 
TCCCATTCTT CGGACACAGG ATCAGGTCCT ACTAAAATGC ACAATGATCA AAGTTCACTA 
GAATATTATA AAGAAGAAGG AAAAACAGAA AATACCAGAA GTCTATTGCA AACTAAAGGT 
CAAGTATCAG AGTCAGAAAT ACCAAAAGCA AGCCAAATGG AGGAACTGGA AGGCTCACAA 
AAAGGTTGCT ATTTGAAATA CTTAATATAC AATAAAAAGA ATGGTGGTGT AAGTCAAGAA 
TTTGGCAATA AGGTTCTTAT AGATGATGCT GCAGGAACAC TGTTATCCAG AGATTCGACA 
GAACATTCTG AAAGTGATAA AATGCTTGCA GAGCAAGAAA GAGAAACATA TTATGCAAAA 
ACGCAGGCAT CATGGGAATC AAACTTCAAA CCTGAATTAA GTATCAAAGA CAAAGTGGAT 
AGCCAGACTA ACATAGACGT CTCCTTAACA GATGACAAGA TTGGCATTTC ACAGTATAAA 
CATGTGGCTG GTATCCCGTT GAAGAGTTTT GATGCAGTGA CAGTCGACAG TGATCTGGAC 
ACGGTGACAA CAGAACGAGT ACGGAATCAC ATCCGAAAGG CCATGGGAAC CAAGAAAGAT 
CCTGCACACA ACACAAAAAG ATTGAACCGG CATCGCAATG AGCTCTTAAG ATACTCCAGT 
AACGTGTCCA CAGATGATGA GGAAGAGGAG GAATATCAGA AGTCCTTCAC GGAATGGAGG 
AGCAGAAGTC TCAAGAGATG GCACTCAGAC CCACACAAAG CAGCTGAAAG TACCTGGGAA 
CACAGTGTCT CCAGCCTTCC ACCAGATTTA ATGAATTCCC CTGAAATTGG GCAGCACCAC 
AGACTTCTAG AAGATCTTGC AAGAGAGAAA GAGCGTCATG AAGAATATGT TGATAAACTT 
CGCAGGGACA TAGCTCTTGA AGAAGAGAGA TTAGCTCAAA GGAAATCTCA GCACCGGGAA 
GTTGATGAGT CCTTTAATGA TATTCTACAG CAAAAGAAGG AAGCTTACCT AGAGTTGGAG 
TCCCTGCAAG ATTTACTAGA TCAGACTCAA AAGGAGGTTG TGAAAATGGA GACACGCTTA 
AGGGAGAACC AGATCACAAC TGATGAATTC AGGAATGAGC TGGCAGTACT GGAATACAAG 
CGCAGTGAAT GCCTAAAAAA TCTTCAGGAT CTAGAACTGG AACTGGAAAT GACAAAGCAG 
CAGTGCTCAA GTGCCCAGAG CGGCCAGATA GCCAGTCTCC AGTATGAGAT CTCTTCACTG 
ACAGGTGAAC GAGATGAGCT AAAGGCCCGT TTACGGCACC TGGAGGGCAG CCTTTCATTT 
TTGGAACGTC AGGAACTGGA ACGTCAGCTC ACTTCGGCAA AGTCTGAACT CTTTTCAGAA 
CAACGGGCAG CCAGGGCCAG GACTGATAAA CTTCAAGAGA ATTTAGAAGA GTCTCAGTGC 
AAGTTGGAGG ACAAGACTGC AGAATGTGCA GAGATGCAGG AGAAAACCAA GCAGCTTAAA 
TCTCAGCTCC GTGAACTGGA AAAAAAATAT GAAGCCAAAA TTCAGAGTCA GGCAGAAGAG 
GCCACTGAAG AAAAAGATGC ACTGAATAAA CAAGTCACAG ACTTGAGTTC CCACGCAAGG 
GAGCAGAATA CAAAAGTAAC CTCTCTGGAG AAGATACTGT CTGAAAAGGA GCTCGAGCTG 
ATGAAAATGA GAGATATTAT ATCCACCCTA AAAGCCGATA AGGAAGCACA GACGCTGGCC 
ACAGACTGCC TAAAGGAACA ACAAAACAAG CTGGTGATGG AACTGCAGCT TCAGCACCAG 
CACGACAAGG ATATGCAGAT CACAAAGCTT AAAGAGGAGT TGGAATGCCA GAAACTGAAG 
GACATCCAAC AACTTGCAGA AAATATGGAA CAGGCTAAAA TGAAAGCACT ACAGGAACAA 
GCAGAAGCTC TCAGAAAGGA GGCCAATAAA GTTGTAAAGT CCCTGGAGGA AAAAGACAAA 
GAAATTGCTA AACTAAAAGA GGCCCTAAAA TCCCAGAAGG ATTCTATGAA AAAGCTTGCT 
GTAGAGCTGA AGCAGGAAGC AAGAGAGATG GTGCACAATA CACTGCTGCG AGAGCAGAAG 
AAGTGGGAAA AAGAAAAGAA GGATGCACTA CATATACAGA AACACACGTT AGAGGAGGAG 
AGGCTTCGAG CCATGGCGGA TCTGCGTGAA GCTCTAGAGA GGGAGCGTAG AACAAACATG 
ATGTTAGAGA AGAAATCTGT TGACCTCCAG AATATTATTC AGGAACATGA GGTTCAGAAT 
CGCTCACTCC AGCGGGAGAA ACAAGATGCC TTAGATGAAC TCCGTGCTAT TCTAAAAGAC 
GAAAATCAAG AAGACATGAA AAGACTACAG CAGGAACTAG CTAAAGAGCG AGAGCGAGAT 
ATGGAGCGCC TAAAGCTCAG GCTGCATGAG TTGGAGGAAG AGCATCATGT TCTACGAGCT 
GAAAAGAATG AGGCCATCTT CCGTGAAAGA GAAGCCCTAG CCCAGGCTGA ACGTGCTGAG 
AAAACACTGG CTAGAGAGAT TAGTTCTGTA TCAGAGCGTA TCCAGAATTC CATGGGCCGG 
ACCAGAATAC ATAGTCCTTC ACACAACAGG CATGGCAGTC CCTCTCGCCT CTCTACCAAC 
CAGGCACTGC AGATGCTTCA TGGAGTATGT GATGAAACCA ACCAGCTTGT ACATGAATTA 
CAGCAGGAAG TAGAAGCTCA GAAGCGCACA GTTCTTCATG TGCAGAGAGA AAAGGAGCGA 
GAATTACAGC AACAGAAAGA GCAGTTGCAA TTAGACAAGG AGAAGGCGCT TGAATTAATG 
AAGGAAAGGC TTATACAGGA GCACATTGAA GAAATCACCA ACCTGCAAAG AGACCAGCTT 
AGGGATTCTA GTAGTGCGGA GAGTCAATCT TTGCGGCAGC AACTGCGGGA GAAGGATAAT 
GAGCTGCGCG CTATACAAAG AAATATGGGC AAATGGAAAG ATGAAACTGC TGCAAAGCTT 
GCTCGCAAAT TTGAAGAAGA GCTAAACGCA GAATTAGAAA AAAGTCTGTC CAGGAGTAAG 
GCCTCAGAGA GTCACCGATA CACAGAAAGA GCTGGGTCAG GAATGCGAAG GCTTTCAGTG 
CAAGAAGGAA GAGAAAATTC ACACCTCCGC TCATCCTCCA CTCCATCTTT GAATGTGGTA 
GGTGCACAGC ATGACTTTGG TGCGCTAAAG ATCCTACGGA ATCTGCAGGG CAGAGTCAGA 
GAGCTGCGGA CAGATAACAG CTTGTACAAT GGGGGGAGCA TGGAAGATCT AAGTTTACTG 
AGGACTGATC TAGGAAGTCC CTACAAACAA AAGAGACCAT TAATGACTGA AAGATCAATG 
GCACACCTTA AAGGAACCAT TAGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493526 ( XM_002931720.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931766.2 Xenopus tropicalis trichohyalin-like (LOC100493526), transcript variant X1, mRNA.

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CloneID	OXa03497
Clone ID Related Accession (Same CDS sequence)	XM_002931720.5 , XM_018090643.1 , XM_002931720.4
Accession Version	XM_002931720.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trichohyalin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931720.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGATCTG 
GAACAGTCTC ATAGAATTGT TTCTCAGGAA CCCAGACATG AACATTCCAT TGATTCCAGT 
AAAGAAACAA AAAAGTGTAA GAAAGAGGAG TACAAGTGGA GGCTTTCTCA CCTCTTAGGC 
TCTGAGCAAA GCGATGCCCA GGAATACCAC AGTGACAATT CTACAGAAAG TGTGTGCACT 
GAGGACTTTG TAATAAAGTT CAAAAAAGGC ATGATTGACC CAGTAGTTAG TGGTGGTAAT 
GATGATCACA TCATGAGCAG TTCAGAAAAG AGCTATGGAG ATCATAAAGA AACTTTGGGA 
GACATACTTG AGAAGATGGG TCCCCTAATG GAAGACACAG GAGATCCTGT GCCCCTAAAC 
ACAGCAAGAA GAGAAAATTT CTTGCAAAAA TTTAAAGAAG AGTTACAAGT TGCACTATCA 
TTTAGCGATT CAGACCAAGC TGAAGAATGT GCCAGGAACA GGAGAGACAG TTTGGAATCT 
CTGGGAGGAA GAATATCAAG ACTTAGCCAT GCAAAAATTT CAGAGCCTTC AAGCCTGCTA 
AGCTTTGGGA AAGGCAGTTC AGTAGCAAGT TATTTGCCAT CCAAAAGTGC AGGTGATAGC 
CAGAAAGGTT TGGGTTCTAA GGATGATTAC TTAATTACCA CTTATGCTAT TTATCCCAAC 
ACCTTGGAAA ATGCAAAAGT CAGTATGAAG AATCTGCAGG AAACAAACCA GGGTTTAAAG 
GTAGTGGCAA CGGGAGTAAA AAAAACTGGG AAAGATGGTC TAGGGGCCCA AGTGTCAGAG 
TCCCATTCTT CGGACACAGG ATCAGGTCCT ACTAAAATGC ACAATGATCA AAGTTCACTA 
GAATATTATA AAGAAGAAGG AAAAACAGAA AATACCAGAA GTCTATTGCA AACTAAAGGT 
CAAGTATCAG AGTCAGAAAT ACCAAAAGCA AGCCAAATGG AGGAACTGGA AGGCTCACAA 
AAAGGTTGCT ATTTGAAATA CTTAATATAC AATAAAAAGA ATGGTGGTGT AAGTCAAGAA 
TTTGGCAATA AGGTTCTTAT AGATGATGCT GCAGGAACAC TGTTATCCAG AGATTCGACA 
GAACATTCTG AAAGTGATAA AATGCTTGCA GAGCAAGAAA GAGAAACATA TTATGCAAAA 
ACGCAGGCAT CATGGGAATC AAACTTCAAA CCTGAATTAA GTATCAAAGA CAAAGTGGAT 
AGCCAGACTA ACATAGACGT CTCCTTAACA GATGACAAGA TTGGCATTTC ACAGTATAAA 
CATGTGGCTG GTATCCCGTT GAAGAGTTTT GATGCAGTGA CAGTCGACAG TGATCTGGAC 
ACGGTGACAA CAGAACGAGT ACGGAATCAC ATCCGAAAGG CCATGGGAAC CAAGAAAGAT 
CCTGCACACA ACACAAAAAG ATTGAACCGG CATCGCAATG AGCTCTTAAG ATACTCCAGT 
AACGTGTCCA CAGATGATGA GGAAGAGGAG GAATATCAGA AGTCCTTCAC GGAATGGAGG 
AGCAGAAGTC TCAAGAGATG GCACTCAGAC CCACACAAAG CAGCTGAAAG TACCTGGGAA 
CACAGTGTCT CCAGCCTTCC ACCAGATTTA ATGAATTCCC CTGAAATTGG GCAGCACCAC 
AGACTTCTAG AAGATCTTGC AAGAGAGAAA GAGCGTCATG AAGAATATGT TGATAAACTT 
CGCAGGGACA TAGCTCTTGA AGAAGAGAGA TTAGCTCAAA GGAAATCTCA GCACCGGGAA 
GTTGATGAGT CCTTTAATGA TATTCTACAG CAAAAGAAGG AAGCTTACCT AGAGTTGGAG 
TCCCTGCAAG ATTTACTAGA TCAGACTCAA AAGGAGGTTG TGAAAATGGA GACACGCTTA 
AGGGAGAACC AGATCACAAC TGATGAATTC AGGAATGAGC TGGCAGTACT GGAATACAAG 
CGCAGTGAAT GCCTAAAAAA TCTTCAGGAT CTAGAACTGG AACTGGAAAT GACAAAGCAG 
CAGTGCTCAA GTGCCCAGAG CGGCCAGATA GCCAGTCTCC AGTATGAGAT CTCTTCACTG 
ACAGGTGAAC GAGATGAGCT AAAGGCCCGT TTACGGCACC TGGAGGGCAG CCTTTCATTT 
TTGGAACGTC AGGAACTGGA ACGTCAGCTC ACTTCGGCAA AGTCTGAACT CTTTTCAGAA 
CAACGGGCAG CCAGGGCCAG GACTGATAAA CTTCAAGAGA ATTTAGAAGA GTCTCAGTGC 
AAGTTGGAGG ACAAGACTGC AGAATGTGCA GAGATGCAGG AGAAAACCAA GCAGCTTAAA 
TCTCAGCTCC GTGAACTGGA AAAAAAATAT GAAGCCAAAA TTCAGAGTCA GGCAGAAGAG 
GCCACTGAAG AAAAAGATGC ACTGAATAAA CAAGTCACAG ACTTGAGTTC CCACGCAAGG 
GAGCAGAATA CAAAAGTAAC CTCTCTGGAG AAGATACTGT CTGAAAAGGA GCTCGAGCTG 
ATGAAAATGA GAGATATTAT ATCCACCCTA AAAGCCGATA AGGAAGCACA GACGCTGGCC 
ACAGACTGCC TAAAGGAACA ACAAAACAAG CTGGTGATGG AACTGCAGCT TCAGCACCAG 
CACGACAAGG ATATGCAGAT CACAAAGCTT AAAGAGGAGT TGGAATGCCA GAAACTGAAG 
GACATCCAAC AACTTGCAGA AAATATGGAA CAGGCTAAAA TGAAAGCACT ACAGGAACAA 
GCAGAAGCTC TCAGAAAGGA GGCCAATAAA GTTGTAAAGT CCCTGGAGGA AAAAGACAAA 
GAAATTGCTA AACTAAAAGA GGCCCTAAAA TCCCAGAAGG ATTCTATGAA AAAGCTTGCT 
GTAGAGCTGA AGCAGGAAGC AAGAGAGATG GTGCACAATA CACTGCTGCG AGAGCAGAAG 
AAGTGGGAAA AAGAAAAGAA GGATGCACTA CATATACAGA AACACACGTT AGAGGAGGAG 
AGGCTTCGAG CCATGGCGGA TCTGCGTGAA GCTCTAGAGA GGGAGCGTAG AACAAACATG 
ATGTTAGAGA AGAAATCTGT TGACCTCCAG AATATTATTC AGGAACATGA GGTTCAGAAT 
CGCTCACTCC AGCGGGAGAA ACAAGATGCC TTAGATGAAC TCCGTGCTAT TCTAAAAGAC 
GAAAATCAAG AAGACATGAA AAGACTACAG CAGGAACTAG CTAAAGAGCG AGAGCGAGAT 
ATGGAGCGCC TAAAGCTCAG GCTGCATGAG TTGGAGGAAG AGCATCATGT TCTACGAGCT 
GAAAAGAATG AGGCCATCTT CCGTGAAAGA GAAGCCCTAG CCCAGGCTGA ACGTGCTGAG 
AAAACACTGG CTAGAGAGAT TAGTTCTGTA TCAGAGCGTA TCCAGAATTC CATGGGCCGG 
ACCAGAATAC ATAGTCCTTC ACACAACAGG CATGGCAGTC CCTCTCGCCT CTCTACCAAC 
CAGGCACTGC AGATGCTTCA TGGAGTATGT GATGAAACCA ACCAGCTTGT ACATGAATTA 
CAGCAGGAAG TAGAAGCTCA GAAGCGCACA GTTCTTCATG TGCAGAGAGA AAAGGAGCGA 
GAATTACAGC AACAGAAAGA GCAGTTGCAA TTAGACAAGG AGAAGGCGCT TGAATTAATG 
AAGGAAAGGC TTATACAGGA GCACATTGAA GAAATCACCA ACCTGCAAAG AGACCAGCTT 
AGGGATTCTA GTAGTGCGGA GAGTCAATCT TTGCGGCAGC AACTGCGGGA GAAGGATAAT 
GAGCTGCGCG CTATACAAAG AAATATGGGC AAATGGAAAG ATGAAACTGC TGCAAAGCTT 
GCTCGCAAAT TTGAAGAAGA GCTAAACGCA GAATTAGAAA AAAGTCTGTC CAGGAGTAAG 
GCCTCAGAGA GTCACCGATA CACAGAAAGA GCTGGGTCAG GAATGCGAAG GCTTTCAGTG 
CAAGAAGGAA GAGAAAATTC ACACCTCCGC TCATCCTCCA CTCCATCTTT GAATGTGGTA 
GGTGCACAGC ATGACTTTGG TGCGCTAAAG ATCCTACGGA ATCTGCAGGG CAGAGTCAGA 
GAGCTGCGGA CAGATAACAG CTTGTACAAT GGGGGGAGCA TGGAAGATCT AAGTTTACTG 
AGGACTGATC TAGGAAGTCC CTACAAACAA AAGAGACCAT TAATGACTGA AAGATCAATG 
GCACACCTTA AAGGAACCAT TAGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931766.2
CDS	201..4610
Translation	MTGMAAGKGTDFDDSAEEDTQLKLQMLLAPSQKSEDKQKDHCDYLLDALDEQLDQLQPRSSFKKVEDNRPKKLENSSADLEQSHRIVSQEPRHEHSIDSSKETKKCKKEEYKWRLSHLLGSEQSDAQEYHSDNSTESVCTEDFVIKFKKGMIDPVVSGGNDDHIMSSSEKSYGDHKETLGDILEKMGPLMEDTGDPVPLNTARRENFLQKFKEELQVALSFSDSDQAEECARNRRDSLESLGGRISRLSHAKISEPSSLLSFGKGSSVASYLPSKSAGDSQKGLGSKDDYLITTYAIYPNTLENAKVSMKNLQETNQGLKVVATGVKKTGKDGLGAQVSESHSSDTGSGPTKMHNDQSSLEYYKEEGKTENTRSLLQTKGQVSESEIPKASQMEELEGSQKGCYLKYLIYNKKNGGVSQEFGNKVLIDDAAGTLLSRDSTEHSESDKMLAEQERETYYAKTQASWESNFKPELSIKDKVDSQTNIDVSLTDDKIGISQYKHVAGIPLKSFDAVTVDSDLDTVTTERVRNHIRKAMGTKKDPAHNTKRLNRHRNELLRYSSNVSTDDEEEEEYQKSFTEWRSRSLKRWHSDPHKAAESTWEHSVSSLPPDLMNSPEIGQHHRLLEDLAREKERHEEYVDKLRRDIALEEERLAQRKSQHREVDESFNDILQQKKEAYLELESLQDLLDQTQKEVVKMETRLRENQITTDEFRNELAVLEYKRSECLKNLQDLELELEMTKQQCSSAQSGQIASLQYEISSLTGERDELKARLRHLEGSLSFLERQELERQLTSAKSELFSEQRAARARTDKLQENLEESQCKLEDKTAECAEMQEKTKQLKSQLRELEKKYEAKIQSQAEEATEEKDALNKQVTDLSSHAREQNTKVTSLEKILSEKELELMKMRDIISTLKADKEAQTLATDCLKEQQNKLVMELQLQHQHDKDMQITKLKEELECQKLKDIQQLAENMEQAKMKALQEQAEALRKEANKVVKSLEEKDKEIAKLKEALKSQKDSMKKLAVELKQEAREMVHNTLLREQKKWEKEKKDALHIQKHTLEEERLRAMADLREALERERRTNMMLEKKSVDLQNIIQEHEVQNRSLQREKQDALDELRAILKDENQEDMKRLQQELAKERERDMERLKLRLHELEEEHHVLRAEKNEAIFREREALAQAERAEKTLAREISSVSERIQNSMGRTRIHSPSHNRHGSPSRLSTNQALQMLHGVCDETNQLVHELQQEVEAQKRTVLHVQREKERELQQQKEQLQLDKEKALELMKERLIQEHIEEITNLQRDQLRDSSSAESQSLRQQLREKDNELRAIQRNMGKWKDETAAKLARKFEEELNAELEKSLSRSKASESHRYTERAGSGMRRLSVQEGRENSHLRSSSTPSLNVVGAQHDFGALKILRNLQGRVRELRTDNSLYNGGSMEDLSLLRTDLGSPYKQKRPLMTERSMAHLKGTIRK

Target ORF information:

RefSeq Version	XM_002931720.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trichohyalin-like (LOC100493526), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931720.4

ORF Insert Sequence:

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGATCTG 
GAACAGTCTC ATAGAATTGT TTCTCAGGAA CCCAGACATG AACATTCCAT TGATTCCAGT 
AAAGAAACAA AAAAGTGTAA GAAAGAGGAG TACAAGTGGA GGCTTTCTCA CCTCTTAGGC 
TCTGAGCAAA GCGATGCCCA GGAATACCAC AGTGACAATT CTACAGAAAG TGTGTGCACT 
GAGGACTTTG TAATAAAGTT CAAAAAAGGC ATGATTGACC CAGTAGTTAG TGGTGGTAAT 
GATGATCACA TCATGAGCAG TTCAGAAAAG AGCTATGGAG ATCATAAAGA AACTTTGGGA 
GACATACTTG AGAAGATGGG TCCCCTAATG GAAGACACAG GAGATCCTGT GCCCCTAAAC 
ACAGCAAGAA GAGAAAATTT CTTGCAAAAA TTTAAAGAAG AGTTACAAGT TGCACTATCA 
TTTAGCGATT CAGACCAAGC TGAAGAATGT GCCAGGAACA GGAGAGACAG TTTGGAATCT 
CTGGGAGGAA GAATATCAAG ACTTAGCCAT GCAAAAATTT CAGAGCCTTC AAGCCTGCTA 
AGCTTTGGGA AAGGCAGTTC AGTAGCAAGT TATTTGCCAT CCAAAAGTGC AGGTGATAGC 
CAGAAAGGTT TGGGTTCTAA GGATGATTAC TTAATTACCA CTTATGCTAT TTATCCCAAC 
ACCTTGGAAA ATGCAAAAGT CAGTATGAAG AATCTGCAGG AAACAAACCA GGGTTTAAAG 
GTAGTGGCAA CGGGAGTAAA AAAAACTGGG AAAGATGGTC TAGGGGCCCA AGTGTCAGAG 
TCCCATTCTT CGGACACAGG ATCAGGTCCT ACTAAAATGC ACAATGATCA AAGTTCACTA 
GAATATTATA AAGAAGAAGG AAAAACAGAA AATACCAGAA GTCTATTGCA AACTAAAGGT 
CAAGTATCAG AGTCAGAAAT ACCAAAAGCA AGCCAAATGG AGGAACTGGA AGGCTCACAA 
AAAGGTTGCT ATTTGAAATA CTTAATATAC AATAAAAAGA ATGGTGGTGT AAGTCAAGAA 
TTTGGCAATA AGGTTCTTAT AGATGATGCT GCAGGAACAC TGTTATCCAG AGATTCGACA 
GAACATTCTG AAAGTGATAA AATGCTTGCA GAGCAAGAAA GAGAAACATA TTATGCAAAA 
ACGCAGGCAT CATGGGAATC AAACTTCAAA CCTGAATTAA GTATCAAAGA CAAAGTGGAT 
AGCCAGACTA ACATAGACGT CTCCTTAACA GATGACAAGA TTGGCATTTC ACAGTATAAA 
CATGTGGCTG GTATCCCGTT GAAGAGTTTT GATGCAGTGA CAGTCGACAG TGATCTGGAC 
ACGGTGACAA CAGAACGAGT ACGGAATCAC ATCCGAAAGG CCATGGGAAC CAAGAAAGAT 
CCTGCACACA ACACAAAAAG ATTGAACCGG CATCGCAATG AGCTCTTAAG ATACTCCAGT 
AACGTGTCCA CAGATGATGA GGAAGAGGAG GAATATCAGA AGTCCTTCAC GGAATGGAGG 
AGCAGAAGTC TCAAGAGATG GCACTCAGAC CCACACAAAG CAGCTGAAAG TACCTGGGAA 
CACAGTGTCT CCAGCCTTCC ACCAGATTTA ATGAATTCCC CTGAAATTGG GCAGCACCAC 
AGACTTCTAG AAGATCTTGC AAGAGAGAAA GAGCGTCATG AAGAATATGT TGATAAACTT 
CGCAGGGACA TAGCTCTTGA AGAAGAGAGA TTAGCTCAAA GGAAATCTCA GCACCGGGAA 
GTTGATGAGT CCTTTAATGA TATTCTACAG CAAAAGAAGG AAGCTTACCT AGAGTTGGAG 
TCCCTGCAAG ATTTACTAGA TCAGACTCAA AAGGAGGTTG TGAAAATGGA GACACGCTTA 
AGGGAGAACC AGATCACAAC TGATGAATTC AGGAATGAGC TGGCAGTACT GGAATACAAG 
CGCAGTGAAT GCCTAAAAAA TCTTCAGGAT CTAGAACTGG AACTGGAAAT GACAAAGCAG 
CAGTGCTCAA GTGCCCAGAG CGGCCAGATA GCCAGTCTCC AGTATGAGAT CTCTTCACTG 
ACAGGTGAAC GAGATGAGCT AAAGGCCCGT TTACGGCACC TGGAGGGCAG CCTTTCATTT 
TTGGAACGTC AGGAACTGGA ACGTCAGCTC ACTTCGGCAA AGTCTGAACT CTTTTCAGAA 
CAACGGGCAG CCAGGGCCAG GACTGATAAA CTTCAAGAGA ATTTAGAAGA GTCTCAGTGC 
AAGTTGGAGG ACAAGACTGC AGAATGTGCA GAGATGCAGG AGAAAACCAA GCAGCTTAAA 
TCTCAGCTCC GTGAACTGGA AAAAAAATAT GAAGCCAAAA TTCAGAGTCA GGCAGAAGAG 
GCCACTGAAG AAAAAGATGC ACTGAATAAA CAAGTCACAG ACTTGAGTTC CCACGCAAGG 
GAGCAGAATA CAAAAGTAAC CTCTCTGGAG AAGATACTGT CTGAAAAGGA GCTCGAGCTG 
ATGAAAATGA GAGATATTAT ATCCACCCTA AAAGCCGATA AGGAAGCACA GACGCTGGCC 
ACAGACTGCC TAAAGGAACA ACAAAACAAG CTGGTGATGG AACTGCAGCT TCAGCACCAG 
CACGACAAGG ATATGCAGAT CACAAAGCTT AAAGAGGAGT TGGAATGCCA GAAACTGAAG 
GACATCCAAC AACTTGCAGA AAATATGGAA CAGGCTAAAA TGAAAGCACT ACAGGAACAA 
GCAGAAGCTC TCAGAAAGGA GGCCAATAAA GTTGTAAAGT CCCTGGAGGA AAAAGACAAA 
GAAATTGCTA AACTAAAAGA GGCCCTAAAA TCCCAGAAGG ATTCTATGAA AAAGCTTGCT 
GTAGAGCTGA AGCAGGAAGC AAGAGAGATG GTGCACAATA CACTGCTGCG AGAGCAGAAG 
AAGTGGGAAA AAGAAAAGAA GGATGCACTA CATATACAGA AACACACGTT AGAGGAGGAG 
AGGCTTCGAG CCATGGCGGA TCTGCGTGAA GCTCTAGAGA GGGAGCGTAG AACAAACATG 
ATGTTAGAGA AGAAATCTGT TGACCTCCAG AATATTATTC AGGAACATGA GGTTCAGAAT 
CGCTCACTCC AGCGGGAGAA ACAAGATGCC TTAGATGAAC TCCGTGCTAT TCTAAAAGAC 
GAAAATCAAG AAGACATGAA AAGACTACAG CAGGAACTAG CTAAAGAGCG AGAGCGAGAT 
ATGGAGCGCC TAAAGCTCAG GCTGCATGAG TTGGAGGAAG AGCATCATGT TCTACGAGCT 
GAAAAGAATG AGGCCATCTT CCGTGAAAGA GAAGCCCTAG CCCAGGCTGA ACGTGCTGAG 
AAAACACTGG CTAGAGAGAT TAGTTCTGTA TCAGAGCGTA TCCAGAATTC CATGGGCCGG 
ACCAGAATAC ATAGTCCTTC ACACAACAGG CATGGCAGTC CCTCTCGCCT CTCTACCAAC 
CAGGCACTGC AGATGCTTCA TGGAGTATGT GATGAAACCA ACCAGCTTGT ACATGAATTA 
CAGCAGGAAG TAGAAGCTCA GAAGCGCACA GTTCTTCATG TGCAGAGAGA AAAGGAGCGA 
GAATTACAGC AACAGAAAGA GCAGTTGCAA TTAGACAAGG AGAAGGCGCT TGAATTAATG 
AAGGAAAGGC TTATACAGGA GCACATTGAA GAAATCACCA ACCTGCAAAG AGACCAGCTT 
AGGGATTCTA GTAGTGCGGA GAGTCAATCT TTGCGGCAGC AACTGCGGGA GAAGGATAAT 
GAGCTGCGCG CTATACAAAG AAATATGGGC AAATGGAAAG ATGAAACTGC TGCAAAGCTT 
GCTCGCAAAT TTGAAGAAGA GCTAAACGCA GAATTAGAAA AAAGTCTGTC CAGGAGTAAG 
GCCTCAGAGA GTCACCGATA CACAGAAAGA GCTGGGTCAG GAATGCGAAG GCTTTCAGTG 
CAAGAAGGAA GAGAAAATTC ACACCTCCGC TCATCCTCCA CTCCATCTTT GAATGTGGTA 
GGTGCACAGC ATGACTTTGG TGCGCTAAAG ATCCTACGGA ATCTGCAGGG CAGAGTCAGA 
GAGCTGCGGA CAGATAACAG CTTGTACAAT GGGGGGAGCA TGGAAGATCT AAGTTTACTG 
AGGACTGATC TAGGAAGTCC CTACAAACAA AAGAGACCAT TAATGACTGA AAGATCAATG 
GCACACCTTA AAGGAACCAT TAGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493526 ( XM_012968620.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824074.1 Xenopus tropicalis trichohyalin-like (LOC100493526), transcript variant X3, mRNA.

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CloneID	OXa35700
Clone ID Related Accession (Same CDS sequence)	XM_012968620.2
Accession Version	XM_012968620.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	trichohyalin-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968620.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGATCTG 
GAACAGTCTC ATAGAATTGT TTCTCAGGAA CCCAGACATG AACATTCCAT TGATTCCAGT 
AAAGAAACAA AAAAGTGTAA GAAAGAGGAG TACAAGTGGA GGCTTTCTCA CCTCTTAGGC 
TCTGAGCAAA GCGATGCCCA GGAATACCAC AGTGACAATT CTACAGAAAG TGTGTGCACT 
GAGGACTTTG TAATAAAGTT CAAAAAAGGC ATGATTGACC CAGTAGTTAG TGGTGGTAAT 
GATGATCACA TCATGAGCAG TTCAGAAAAG AGCTATGGAG ATCATAAAGA AACTTTGGGA 
GACATACTTG AGAAGATGGG TCCCCTAATG GAAGACACAG GAGATCCTGT GCCCCTAAAC 
ACAGCAAGAA GAGAAAATTT CTTGCAAAAA TTTAAAGAAG AGTTACAAGT TGCACTATCA 
TTTAGCGATT CAGACCAAGC TGAAGAATGT GCCAGGAACA GGAGAGACAG TTTGGAATCT 
CTGGGAGGAA GAATATCAAG ACTTAGCCAT GCAAAAATTT CAGAGCCTTC AAGCCTGCTA 
AGCTTTGGGA AAGGCAGTTC AGTAGCAAGT TATTTGCCAT CCAAAAGTGC AGGTGATAGC 
CAGAAAGGTT TGGGTTCTAA GGATGATTAC TTAATTACCA CTTATGCTAT TTATCCCAAC 
ACCTTGGAAA ATGCAAAAGT CAGTATGAAG AATCTGCAGG AAACAAACCA GGGTTTAAAG 
GTAGTGGCAA CGGGAGTAAA AAAAACTGGG AAAGATGGTC TAGGGGCCCA AGTGTCAGAG 
TCCCATTCTT CGGACACAGG ATCAGGTCCT ACTAAAATGC ACAATGATCA AAGTTCACTA 
GAATATTATA AAGAAGAAGG AAAAACAGAA AATACCAGAA GTCTATTGCA AACTAAAGGT 
CAAGTATCAG AGTCAGAAAT ACCAAAAGCA AGCCAAATGG AGGAACTGGA AGGCTCACAA 
AAAGGTTGCT ATTTGAAATA CTTAATATAC AATAAAAAGA ATGGTGGTGT AAGTCAAGAA 
TTTGGCAATA AGGTTCTTAT AGATGATGCT GCAGGAACAC TGTTATCCAG AGATTCGACA 
GAACATTCTG AAAGTGATAA AATGCTTGCA GAGCAAGAAA GAGAAACATA TTATGCAAAA 
ACGCAGGCAT CATGGGAATC AAACTTCAAA CCTGAATTAA GTATCAAAGA CAAAGTGGAT 
AGCCAGACTA ACATAGACGT CTCCTTAACA GATGACAAGA TTGGCATTTC ACAGTATAAA 
CATGTGGCTG GTATCCCGTT GAAGAGTTTT GATGCAGTGA CAGTCGACAG TGATCTGGAC 
ACGGTGACAA CAGAACGAGT ACGGAATCAC ATCCGAAAGG CCATGGGAAC CAAGAAAGAT 
CCTGCACACA ACACAAAAAG ATTGAACCGG CATCGCAATG AGCTCTTAAG ATACTCCAGT 
AACGTGTCCA CAGATGATGA GGAAGAGGAG GAATATCAGA AGTCCTTCAC GGAATGGAGG 
AGCAGAAGTC TCAAGAGATG GCACTCAGAC CCACACAAAG CAGCTGAAAG TACCTGGGAA 
CACAGTGTCT CCAGCCTTCC ACCAGATTTA ATGAATTCCC CTGAAATTGG GCAGCACCAC 
AGACTTCTAG AAGATCTTGC AAGAGAGAAA GAGCGTCATG AAGAATATGT TGATAAACTT 
CGCAGGGACA TAGCTCTTGA AGAAGAGAGA TTAGCTCAAA GGAAATCTCA GCACCGGGAA 
GTTGATGAGT CCTTTAATGA TATTCTACAG CAAAAGAAGG AAGCTTACCT AGAGTTGGAG 
TCCCTGCAAG ATTTACTAGA TCAGACTCAA AAGGAGGTTG TGAAAATGGA GACACGCTTA 
AGGGAGAACC AGATCACAAC TGATGAATTC AGGAATGAGC TGGCAGTACT GGAATACAAG 
CGCAGTGAAT GCCTAAAAAA TCTTCAGGAT CTAGAACTGG AACTGGAAAT GACAAAGCAG 
CAGTGCTCAA GTGCCCAGAG CGGCCAGATA GCCAGTCTCC AGTATGAGAT CTCTTCACTG 
ACAGGTGAAC GAGATGAGCT AAAGGCCCGT TTACGGCACC TGGAGGGCAG CCTTTCATTT 
TTGGAACGTC AGGAACTGGA ACGTCAGCTC ACTTCGGCAA AGTCTGAACT CTTTTCAGAA 
CAACGGGCAG CCAGGGCCAG GACTGATAAA CTTCAAGAGA ATTTAGAAGA GTCTCAGTGC 
AAGTTGGAGG ACAAGACTGC AGAATGTGCA GAGATGCAGG AGAAAACCAA GCAGCTTAAA 
TCTCAGCTCC GTGAACTGGA AAAAAAATAT GAAGCCAAAA TTCAGAGTCA GGCAGAAGAG 
GCCACTGAAG AAAAAGATGC ACTGAATAAA CAAGTCACAG ACTTGAGTTC CCACGCAAGG 
GAGCAGAATA CAAAAGTAAC CTCTCTGGAG AAGATACTGT CTGAAAAGGA GCTCGAGCTG 
ATGAAAATGA GAGATATTAT ATCCACCCTA AAAGCCGATA AGGAAGCACA GACGCTGGCC 
ACAGACTGCC TAAAGGAACA ACAAAACAAG CTGGTGATGG AACTGCAGCT TCAGCACCAG 
CACGACAAGG ATATGCAGAT CACAAAGCTT AAAGAGGAGT TGGAATGCCA GAAACTGAAG 
GACATCCAAC AACTTGCAGA AAATATGGAA CAGGCTAAAA TGAAAGCACT ACAGGAACAA 
GCAGAAGCTC TCAGAAAGGA GGCCAATAAA GTTGTAAAGT CCCTGGAGGA AAAAGACAAA 
GAAATTGCTA AACTAAAAGA GGCCCTAAAA TCCCAGAAGG ATTCTATGAA AAAGCTTGCT 
GTAGAGCTGA AGCAGGAAGC AAGAGAGATG GTGCACAATA CACTGCTGCG AGAGCAGAAG 
AAGTGGGAAA AAGAAAAGAA GGATGCACTA CATATACAGA AACACACGTT AGAGGAGGAG 
AGGCTTCGAG CCATGGCGGA TCTGCGTGAA GCTCTAGAGA GGGAGCGTAG AACAAACATG 
ATGTTAGAGA AGAAATCTGT TGACCTCCAG AATATTATTC AGGAACATGA GGTTCAGAAT 
CGCTCACTCC AGCGGGAGAA ACAAGATGCC TTAGATGAAC TCCGTGCTAT TCTAAAAGAC 
GAAAATCAAG AAGACATGAA AAGACTACAG CAGGAACTAG CTAAAGAGCG AGAGCGAGAT 
ATGGAGCGCC TAAAGCTCAG GCTGCATGAG TTGGAGGAAG AGCATCATGT TCTACGAGCT 
GAAAAGAATG AGGCCATCTT CCGTGAAAGA GAAGCCCTAG CCCAGGCTGA ACGTGCTGAG 
AAAACACTGG CTAGAGAGAT TAGTTCTGTA TCAGAGCGTA TCCAGAATTC CATGGGCCGG 
ACCAGAATAC ATAGTCCTTC ACACAACAGG CATGGCAGTC CCTCTCGCCT CTCTACCAAC 
CAGGCACTGC AGATGCTTCA TGGAGTATGT GATGAAACCA ACCAGCTTGT ACATGAATTA 
CAGCAGGAAG TAGAAGCTCA GAAGCGCACA GTTCTTCATG TGCAGAGAGA AAAGGAGCGA 
GAATTACAGC AACAGAAAGA GCAGTTGCAA TTAGACAAGG AGAAGGCGCT TGAATTAATG 
AAGGAAAGGC TTATACAGGA GCACATTGAA GAAATCACCA ACCTGCAAAG AGACCAGCTT 
AGGGATTCTA GTAGTGCGGA GAGTCAATCT TTGCGGCAGC AACTGCGGGA GAAGGATAAT 
GAGCTGCGCG CTATACAAAG AAATATGGGC AAATGGAAAG ATGAAACTGC TGCAAAGCTT 
GCTCGCAAAT TTGAAGAAGA GCTAAACGCA GAATTAGAAA AATGCAAAGT GGAGTTTTTA 
AAAGAAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824074.1
CDS	202..4293
Translation	MTGMAAGKGTDFDDSAEEDTQLKLQMLLAPSQKSEDKQKDHCDYLLDALDEQLDQLQPRSSFKKVEDNRPKKLENSSADLEQSHRIVSQEPRHEHSIDSSKETKKCKKEEYKWRLSHLLGSEQSDAQEYHSDNSTESVCTEDFVIKFKKGMIDPVVSGGNDDHIMSSSEKSYGDHKETLGDILEKMGPLMEDTGDPVPLNTARRENFLQKFKEELQVALSFSDSDQAEECARNRRDSLESLGGRISRLSHAKISEPSSLLSFGKGSSVASYLPSKSAGDSQKGLGSKDDYLITTYAIYPNTLENAKVSMKNLQETNQGLKVVATGVKKTGKDGLGAQVSESHSSDTGSGPTKMHNDQSSLEYYKEEGKTENTRSLLQTKGQVSESEIPKASQMEELEGSQKGCYLKYLIYNKKNGGVSQEFGNKVLIDDAAGTLLSRDSTEHSESDKMLAEQERETYYAKTQASWESNFKPELSIKDKVDSQTNIDVSLTDDKIGISQYKHVAGIPLKSFDAVTVDSDLDTVTTERVRNHIRKAMGTKKDPAHNTKRLNRHRNELLRYSSNVSTDDEEEEEYQKSFTEWRSRSLKRWHSDPHKAAESTWEHSVSSLPPDLMNSPEIGQHHRLLEDLAREKERHEEYVDKLRRDIALEEERLAQRKSQHREVDESFNDILQQKKEAYLELESLQDLLDQTQKEVVKMETRLRENQITTDEFRNELAVLEYKRSECLKNLQDLELELEMTKQQCSSAQSGQIASLQYEISSLTGERDELKARLRHLEGSLSFLERQELERQLTSAKSELFSEQRAARARTDKLQENLEESQCKLEDKTAECAEMQEKTKQLKSQLRELEKKYEAKIQSQAEEATEEKDALNKQVTDLSSHAREQNTKVTSLEKILSEKELELMKMRDIISTLKADKEAQTLATDCLKEQQNKLVMELQLQHQHDKDMQITKLKEELECQKLKDIQQLAENMEQAKMKALQEQAEALRKEANKVVKSLEEKDKEIAKLKEALKSQKDSMKKLAVELKQEAREMVHNTLLREQKKWEKEKKDALHIQKHTLEEERLRAMADLREALERERRTNMMLEKKSVDLQNIIQEHEVQNRSLQREKQDALDELRAILKDENQEDMKRLQQELAKERERDMERLKLRLHELEEEHHVLRAEKNEAIFREREALAQAERAEKTLAREISSVSERIQNSMGRTRIHSPSHNRHGSPSRLSTNQALQMLHGVCDETNQLVHELQQEVEAQKRTVLHVQREKERELQQQKEQLQLDKEKALELMKERLIQEHIEEITNLQRDQLRDSSSAESQSLRQQLREKDNELRAIQRNMGKWKDETAAKLARKFEEELNAELEKCKVEFLKER

Target ORF information:

RefSeq Version	XM_012968620.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis trichohyalin-like (LOC100493526), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968620.2

ORF Insert Sequence:

ATGACTGGCA TGGCGGCTGG CAAGGGCACA GATTTTGATG ATTCTGCTGA AGAAGACACA 
CAGCTAAAAC TTCAGATGCT TCTTGCACCC TCTCAGAAAT CTGAAGACAA ACAAAAGGAT 
CACTGTGATT ACCTGTTGGA TGCTTTGGAT GAACAATTGG ATCAGTTACA GCCACGGTCA 
AGTTTTAAGA AAGTAGAAGA CAACAGACCT AAAAAGTTAG AAAATTCTTC AGCAGATCTG 
GAACAGTCTC ATAGAATTGT TTCTCAGGAA CCCAGACATG AACATTCCAT TGATTCCAGT 
AAAGAAACAA AAAAGTGTAA GAAAGAGGAG TACAAGTGGA GGCTTTCTCA CCTCTTAGGC 
TCTGAGCAAA GCGATGCCCA GGAATACCAC AGTGACAATT CTACAGAAAG TGTGTGCACT 
GAGGACTTTG TAATAAAGTT CAAAAAAGGC ATGATTGACC CAGTAGTTAG TGGTGGTAAT 
GATGATCACA TCATGAGCAG TTCAGAAAAG AGCTATGGAG ATCATAAAGA AACTTTGGGA 
GACATACTTG AGAAGATGGG TCCCCTAATG GAAGACACAG GAGATCCTGT GCCCCTAAAC 
ACAGCAAGAA GAGAAAATTT CTTGCAAAAA TTTAAAGAAG AGTTACAAGT TGCACTATCA 
TTTAGCGATT CAGACCAAGC TGAAGAATGT GCCAGGAACA GGAGAGACAG TTTGGAATCT 
CTGGGAGGAA GAATATCAAG ACTTAGCCAT GCAAAAATTT CAGAGCCTTC AAGCCTGCTA 
AGCTTTGGGA AAGGCAGTTC AGTAGCAAGT TATTTGCCAT CCAAAAGTGC AGGTGATAGC 
CAGAAAGGTT TGGGTTCTAA GGATGATTAC TTAATTACCA CTTATGCTAT TTATCCCAAC 
ACCTTGGAAA ATGCAAAAGT CAGTATGAAG AATCTGCAGG AAACAAACCA GGGTTTAAAG 
GTAGTGGCAA CGGGAGTAAA AAAAACTGGG AAAGATGGTC TAGGGGCCCA AGTGTCAGAG 
TCCCATTCTT CGGACACAGG ATCAGGTCCT ACTAAAATGC ACAATGATCA AAGTTCACTA 
GAATATTATA AAGAAGAAGG AAAAACAGAA AATACCAGAA GTCTATTGCA AACTAAAGGT 
CAAGTATCAG AGTCAGAAAT ACCAAAAGCA AGCCAAATGG AGGAACTGGA AGGCTCACAA 
AAAGGTTGCT ATTTGAAATA CTTAATATAC AATAAAAAGA ATGGTGGTGT AAGTCAAGAA 
TTTGGCAATA AGGTTCTTAT AGATGATGCT GCAGGAACAC TGTTATCCAG AGATTCGACA 
GAACATTCTG AAAGTGATAA AATGCTTGCA GAGCAAGAAA GAGAAACATA TTATGCAAAA 
ACGCAGGCAT CATGGGAATC AAACTTCAAA CCTGAATTAA GTATCAAAGA CAAAGTGGAT 
AGCCAGACTA ACATAGACGT CTCCTTAACA GATGACAAGA TTGGCATTTC ACAGTATAAA 
CATGTGGCTG GTATCCCGTT GAAGAGTTTT GATGCAGTGA CAGTCGACAG TGATCTGGAC 
ACGGTGACAA CAGAACGAGT ACGGAATCAC ATCCGAAAGG CCATGGGAAC CAAGAAAGAT 
CCTGCACACA ACACAAAAAG ATTGAACCGG CATCGCAATG AGCTCTTAAG ATACTCCAGT 
AACGTGTCCA CAGATGATGA GGAAGAGGAG GAATATCAGA AGTCCTTCAC GGAATGGAGG 
AGCAGAAGTC TCAAGAGATG GCACTCAGAC CCACACAAAG CAGCTGAAAG TACCTGGGAA 
CACAGTGTCT CCAGCCTTCC ACCAGATTTA ATGAATTCCC CTGAAATTGG GCAGCACCAC 
AGACTTCTAG AAGATCTTGC AAGAGAGAAA GAGCGTCATG AAGAATATGT TGATAAACTT 
CGCAGGGACA TAGCTCTTGA AGAAGAGAGA TTAGCTCAAA GGAAATCTCA GCACCGGGAA 
GTTGATGAGT CCTTTAATGA TATTCTACAG CAAAAGAAGG AAGCTTACCT AGAGTTGGAG 
TCCCTGCAAG ATTTACTAGA TCAGACTCAA AAGGAGGTTG TGAAAATGGA GACACGCTTA 
AGGGAGAACC AGATCACAAC TGATGAATTC AGGAATGAGC TGGCAGTACT GGAATACAAG 
CGCAGTGAAT GCCTAAAAAA TCTTCAGGAT CTAGAACTGG AACTGGAAAT GACAAAGCAG 
CAGTGCTCAA GTGCCCAGAG CGGCCAGATA GCCAGTCTCC AGTATGAGAT CTCTTCACTG 
ACAGGTGAAC GAGATGAGCT AAAGGCCCGT TTACGGCACC TGGAGGGCAG CCTTTCATTT 
TTGGAACGTC AGGAACTGGA ACGTCAGCTC ACTTCGGCAA AGTCTGAACT CTTTTCAGAA 
CAACGGGCAG CCAGGGCCAG GACTGATAAA CTTCAAGAGA ATTTAGAAGA GTCTCAGTGC 
AAGTTGGAGG ACAAGACTGC AGAATGTGCA GAGATGCAGG AGAAAACCAA GCAGCTTAAA 
TCTCAGCTCC GTGAACTGGA AAAAAAATAT GAAGCCAAAA TTCAGAGTCA GGCAGAAGAG 
GCCACTGAAG AAAAAGATGC ACTGAATAAA CAAGTCACAG ACTTGAGTTC CCACGCAAGG 
GAGCAGAATA CAAAAGTAAC CTCTCTGGAG AAGATACTGT CTGAAAAGGA GCTCGAGCTG 
ATGAAAATGA GAGATATTAT ATCCACCCTA AAAGCCGATA AGGAAGCACA GACGCTGGCC 
ACAGACTGCC TAAAGGAACA ACAAAACAAG CTGGTGATGG AACTGCAGCT TCAGCACCAG 
CACGACAAGG ATATGCAGAT CACAAAGCTT AAAGAGGAGT TGGAATGCCA GAAACTGAAG 
GACATCCAAC AACTTGCAGA AAATATGGAA CAGGCTAAAA TGAAAGCACT ACAGGAACAA 
GCAGAAGCTC TCAGAAAGGA GGCCAATAAA GTTGTAAAGT CCCTGGAGGA AAAAGACAAA 
GAAATTGCTA AACTAAAAGA GGCCCTAAAA TCCCAGAAGG ATTCTATGAA AAAGCTTGCT 
GTAGAGCTGA AGCAGGAAGC AAGAGAGATG GTGCACAATA CACTGCTGCG AGAGCAGAAG 
AAGTGGGAAA AAGAAAAGAA GGATGCACTA CATATACAGA AACACACGTT AGAGGAGGAG 
AGGCTTCGAG CCATGGCGGA TCTGCGTGAA GCTCTAGAGA GGGAGCGTAG AACAAACATG 
ATGTTAGAGA AGAAATCTGT TGACCTCCAG AATATTATTC AGGAACATGA GGTTCAGAAT 
CGCTCACTCC AGCGGGAGAA ACAAGATGCC TTAGATGAAC TCCGTGCTAT TCTAAAAGAC 
GAAAATCAAG AAGACATGAA AAGACTACAG CAGGAACTAG CTAAAGAGCG AGAGCGAGAT 
ATGGAGCGCC TAAAGCTCAG GCTGCATGAG TTGGAGGAAG AGCATCATGT TCTACGAGCT 
GAAAAGAATG AGGCCATCTT CCGTGAAAGA GAAGCCCTAG CCCAGGCTGA ACGTGCTGAG 
AAAACACTGG CTAGAGAGAT TAGTTCTGTA TCAGAGCGTA TCCAGAATTC CATGGGCCGG 
ACCAGAATAC ATAGTCCTTC ACACAACAGG CATGGCAGTC CCTCTCGCCT CTCTACCAAC 
CAGGCACTGC AGATGCTTCA TGGAGTATGT GATGAAACCA ACCAGCTTGT ACATGAATTA 
CAGCAGGAAG TAGAAGCTCA GAAGCGCACA GTTCTTCATG TGCAGAGAGA AAAGGAGCGA 
GAATTACAGC AACAGAAAGA GCAGTTGCAA TTAGACAAGG AGAAGGCGCT TGAATTAATG 
AAGGAAAGGC TTATACAGGA GCACATTGAA GAAATCACCA ACCTGCAAAG AGACCAGCTT 
AGGGATTCTA GTAGTGCGGA GAGTCAATCT TTGCGGCAGC AACTGCGGGA GAAGGATAAT 
GAGCTGCGCG CTATACAAAG AAATATGGGC AAATGGAAAG ATGAAACTGC TGCAAAGCTT 
GCTCGCAAAT TTGAAGAAGA GCTAAACGCA GAATTAGAAA AATGCAAAGT GGAGTTTTTA 
AAAGAAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

LOC100493526 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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