scn4a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following scn4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the scn4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa50621 XM_031894531.1
Latest version!
Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa50618 XM_031894527.1
Latest version!
Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa50618 XM_018089322.2
Latest version!
Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa50618 XM_031894526.1
Latest version!
Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa50619 XM_031894528.1
Latest version!
Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa50620 XM_031894529.1
Latest version!
Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa50620 XM_031894530.1
Latest version!
Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa35367 XM_018089322.1
Latest version!
Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa50621
    Clone ID Related Accession (Same CDS sequence) XM_031894531.1
    Accession Version XM_031894531.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium channel protein type 4 subunit alpha isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGGTATTG
    TTCTCTCGGA TCTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750391.1
    CDS159..4256
    Translation

    Target ORF information:

    RefSeq Version XM_031894531.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894531.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGGTATTG
    TTCTCTCGGA TCTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50618
    Clone ID Related Accession (Same CDS sequence) XM_018089322.2 , XM_031894526.1 , XM_031894527.1
    Accession Version XM_018089322.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium channel protein type 4 subunit alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018089322.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC
    CTGTTGGAAC TGACAGAGGG AACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017944811.2
    CDS158..5707
    Translation

    Target ORF information:

    RefSeq Version XM_018089322.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089322.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC
    CTGTTGGAAC TGACAGAGGG AACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50618
    Clone ID Related Accession (Same CDS sequence) XM_018089322.2 , XM_031894526.1 , XM_031894527.1
    Accession Version XM_031894526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium channel protein type 4 subunit alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC
    CTGTTGGAAC TGACAGAGGG AACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750386.1
    CDS323..5872
    Translation

    Target ORF information:

    RefSeq Version XM_031894526.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC
    CTGTTGGAAC TGACAGAGGG AACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50618
    Clone ID Related Accession (Same CDS sequence) XM_018089322.2 , XM_031894526.1 , XM_031894527.1
    Accession Version XM_031894527.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium channel protein type 4 subunit alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC
    CTGTTGGAAC TGACAGAGGG AACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750387.1
    CDS249..5798
    Translation

    Target ORF information:

    RefSeq Version XM_031894527.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894527.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC
    CTGTTGGAAC TGACAGAGGG AACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50619
    Clone ID Related Accession (Same CDS sequence) XM_031894528.1
    Accession Version XM_031894528.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium channel protein type 4 subunit alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT GTAAGAGAGA CAGATGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750388.1
    CDS158..5668
    Translation

    Target ORF information:

    RefSeq Version XM_031894528.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894528.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT GTAAGAGAGA CAGATGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50620
    Clone ID Related Accession (Same CDS sequence) XM_031894530.1 , XM_031894529.1
    Accession Version XM_031894530.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium channel protein type 4 subunit alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750390.1
    CDS158..5650
    Translation

    Target ORF information:

    RefSeq Version XM_031894530.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894530.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50620
    Clone ID Related Accession (Same CDS sequence) XM_031894530.1 , XM_031894529.1
    Accession Version XM_031894529.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium channel protein type 4 subunit alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750389.1
    CDS158..5650
    Translation

    Target ORF information:

    RefSeq Version XM_031894529.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894529.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCGAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA ATGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTATCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATAAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35367
    Clone ID Related Accession (Same CDS sequence) XM_018089322.1
    Accession Version XM_018089322.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sodium channel protein type 4 subunit alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCTAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA AAGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTTTCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATTAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC
    CTGTTGGAAC TGACAGAGGG AACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017944811.1
    CDS140..5689
    Translation

    Target ORF information:

    RefSeq Version XM_018089322.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sodium channel, voltage gated, type IV alpha subunit (scn4a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089322.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCTGGCA AGGTGCACCC TCCCGGTCCT GATAGTTTGC GTCCATTTAC CAGAGAGTCC 
    TTGGCTGCGA TAGAGAAGCG CATTGAGGAG GACATCGCTC GGAAAGCCAA ACAGATTGAG
    GTGCTCGATG AGAAAGTACC GACACCCAAA AATGGACTGG AGGCAGGAAA GAACTTGCCT
    CTTATATATG GGGATCCCCC ACCCGAACTT ATTGGGATTC CCATAGAGGA ACTAGATTCT
    TTTTACAGCA ACCAAAAGAC ATTTATCGTC TTAAACAAAG GAAAAGCAAT ATTCCGATTC
    ACAGCCACCA AAGCCTTATA TCTACTGGAT CCATTTAATC CAATCAGAAG AGGCGCTATT
    AAAGTCTTAA TCCATTCATT GTTTAGCTTA TTTATTATGT TTACCATCTT AACAAATTGT
    GTTTTTATGA CCATGAGTGA CCCACCACCC TGGTCCAAGA ATGTAGAGTA CACGTTTACT
    GGAATTTACA CCTTTGAGTC CTTAATAAAA ATCCTGGCTC GGGGTTTTTG TATTGACAGT
    TTTACATTCC TCAGGGATCC GTGGAACTGG CTGGATTTCT GTGTCATTGT TATGGCTTAC
    ACCACAGAGT TTGTAGATTT GGGGAATGTG TCTGCCCTTA GGACATTCCG TGTACTCCGT
    GCTCTTAAAA CAATTACAGT TATTCCAGGC CTGAAGACTA TCGTTGGTGC CTTGATCCAA
    TCAGTGAAGA AGCTCGCAGA TGTGATGATC CTCACCCTCT TCTGCCTTGC TGTCTTTGCC
    CTCATTGGCC TTCAGCTCTT TATGGGAAAT CTGAGGCACA AATGTGTCCG ATGGCCACCC
    CCTGATGACT ATTTCATGCT TTATGACAAC AGTACCTTAA ACTCCACACT ATATAATGAT
    ACAATGATCA ACTCCACTCT CAGCAACAGC ACAAATGTGT TCGACAACGA CACCATTGAC
    TGGGGTGCCT ATCTCAACGA TGAAGCAAAT TTTTACTTCC TGGATGGAGC AAATGATGCA
    CTTCTGTGTG GGAACAGCAG TGATGCCGGG AAATGTCCAG AAAGTTACAC ATGTATGAAA
    ACTGGGAGAA ATCCTAATTA TGGGTACACC AGCTACGACT CATTCAACTG GGCTTTCCTT
    TCCCTTTTCC GATTAATGAC ACAGGACTTC TGGGAGAATC TTTTCCAGTT GACACTACGA
    GCAGCTGGTA AAACCTACAT GGTCTTCTTT GTGGTGGTAA TTTTTCTGGG GTCTTTCTAT
    CTGATCAACT TGATCCTTGC TGTGGTGGCC ATGGCATATG CAGAACAGAA TGAAGCCACT
    CTGCAAGAAG AAATAGAAAA GGAGAAAGAG TTCCAGGAGC TTTTGTTGCA GCTAAAGAAA
    CATCAAGAAG AGCAAGCAAG AAGGAAATCC AGCGTTGCCT CAGTTGGACT TCTGAACGCA
    GAAAAGAAAA AAGCTCTTTC AGAGAACACT CTTTCTGGGG ATGGCACAGA AAATATGGAC
    TGCAATGGGC GAACTGTGCC CAAAGTTATA ATTGAACGTG CACCCTCAAT GGAGGATACA
    AGCTCAGCAC AGTCAGACCA ACAGAGGAAT TCTAAAGTTT ATTTGGAGGA GCCAACTCTG
    CAGAAAAGAG CAGAGAGCAC CCACAGTGTC CTATCGGAAG TAGCTCTTGA AGAACTGGAA
    GAGGCTCATC AGAAATGCCC TCCTTGGTGG TACAAGTTTG CTAACACCTT TTTGATTTGG
    GACTGCTGTG ACCTGTGGGT GAAATTTAAG GAATTTGTAA GGTTTGTGGT CATGGATCCC
    TTTGTTGACC TGGGAATCAC CATTTGCATC GTGCTTAACA CCCTTTTTAT GGCCATGGAG
    CACTACCCAA TGACTGAGCA GTTTGAGGGT GTTCTGAATG TAGGAAACTT GGTCTTTACT
    GGCATATTTG CAGCTGAAAT GTTCTTCAAG ATCATAGCCC TGGATCCATA TTACTACTTC
    CAAGTGGGGT GGAACATATT CGACAGCATT ATAGTCACTT TAAGTTTGGT GGAGTTGGGC
    CTTGCAAATG TGGAAGGTCT GTCCGTGCTC AGATCGTTCC GTCTGCTCAG GGTGTTCAAG
    CTAGCCAAAT CATGGCCAAC CCTTAACATG CTTATCAAAA TCATCGGTAA CTCGGTCGGA
    GCACTTGGAA ACCTCACCTT GGTGTTGGCC ATCATTGTCT TCATCTTTGC GGTGGTGGGG
    ATGCAGCTAT TTGGGAAGAG CTACAAGGAA TGTGTGTGCA AGATTGCCTC TGACTGCGAG
    CTACCACGGT GGCACATGCA CGATTTCTTC CACTCTTTTC TCATTGTCTT CCGTATCCTG
    TGCGGGGAGT GGATAGAGAC AATGTGGGAC TGCATGGAAG TGGCAGGGCA AGCAATGTGC
    CTGGTCGTGT TCATGATGGT CATGGTGATT GGTAACCTGG TGGTCCTCAA TCTCTTTTTG
    GCTTTGTTGC TGAGTTCCTT CAGCGCGGAT AACTTGTCGG CTTCGGACGA CGATGGGGAG
    ATGAACAACC TGCAGATCGC CATCGGAAGG ATCACCAGGG GCATTGACTT TGTGAAGAAA
    ACCTTCTTCT CCATCCTACA TTACAAGAGG AAGGCAAAAG CTGAGCAGGA GGAAGAGGAC
    CCTAACAAAG AAAACATTGC TCTGAACCAT GTAGAGACAG AACCAGAACC CAAGATAGAA
    CTAGAACCCA GGACAGAACC AGAACCCAAA AAAGAACCTC CACTGATTGA TGGCGTGACT
    AAAAAACGTT ATTACATGGA TGAGTTTGAC CACGCTAATT TTTTTAACAA CCCACACATG
    AGAATAGAAG TACCTATTGC CTCAGAAGAG TCCGACTTCG ACATTCCAGA CACGGATGAG
    TTTAGCACAC AGTCGGAGGT AGAGGAAGGG ATTTTACACC ACAAGAAGTA CGATGATGGC
    TCGTCAGTGT GTAGCACAGC AGATTATAAA CCTCCTGAAC CTGAAGAGGA AGAGGAGGCC
    TTTGAACACA AAGAAGACGA AGGCCCAGAA GAATGCTTTA CTGCTGACTG TGTACGAAGA
    TGCCCTTGTC TTTATGTCGA CATCACAACA GAAAAAGGGA AAATTTGGTG GAACATTCGG
    AAAACATGTT TCCAGATTGT GGAGCATAAC TGGTTTGAAA CCTTCATCAT CTTCATGATC
    TTACTCAGTA GTGGAGCACT GGCATTTGAG GATATCTACA TTGAACAGAG GCAAGTGATA
    AAGACTATCC TGGAGTACGC TGATAAAGTC TTCACATATA TTTTTGTGTT TGAAATGCTG
    CTAAAGTGGA CAGCGTATGG CTTCAAGATC TATTTCACGA ATGCCTGGTG CTGGCTGGAC
    TTCCTAATTG TTGACGTGTC TTTAATCAGC CTGACAGCCA ACTGGCTGGG CTACTCGGAA
    CTTGGTCCCA TCAAATCCCT TAGAACGCTG AGGGCCCTTA GGCCGTTACG AGCGTTATCA
    AGATTTGAAG GCATGAGGGT GGTTGTAAAC GCCCTTCTGG GAGCCATTCC TTCTATCATG
    AATGTCCTGC TTGTTTGTCT GATCTTCTGG CTCATCTTCA GCATCATGGG TGTTAACCTC
    TTTGCTGGGA AATATTATCG ATGCATCAAC ACGACCACGT CAGAATTATT TGAGATCGAT
    GTGGTCAACA ACCGCTCGGA CTGCCTCGCT TTAAACAACT CCGAGCAAGT GCGATGGGTT
    AATGTCAAAG TGAATTTTGA CAATGTGGGC CTTGGGTATC TCTCCCTTCT ACAAGTGGCA
    ACCTTCAAAG GCTGGATGGA TATCATGTAT GCCGCTGTAG ATTCCCGGAA TATTGAGGAT
    CAGCCACAAT ATGAAATAAA TCTTTATATG TATTTGTACT TTGTAATATT CATTATTTTT
    GGAGCTTTCT TCACTCTAAA CCTGTTTATC GGTGTCATTA TCGACAACTT CAATCAGCAA
    AAGAAAAAGT TTGGAGGTAA GGATATCTTT ATGACTGAGG AACAGAAGAA ATACTACAAT
    GCTATGAAAA AGCTGGGATC CAAAAAACCC CAAAAGCCAA TTCCAAGACC CACGAACAAA
    ATTCAAGGTT TGGTGTATGA CTTTGTCACA AAGCAGCTCT TTGACATTGT GATTATGATT
    TTAATTTGCC TCAACATGGT GACGATGATG ATTGAAACAG ATGACCAGAG TCAGGAAAAA
    ACTGATATTC TTTTCAAGAT CAATCTGGTG TTCATTGTCA TCTTCACAGC CGAATGTGTT
    CTCAAAATGT TTGCGCTGAG GTACTACTTT TTCACAATAG GATGGAACAT ATTTGACTTT
    GTGGTGGTCA TCCTTTCTAT TGTTGGTATT GTTCTCTCGG ATCTCATAGC AGAGTACTTT
    GTTTCACCAA CACTTTTCAG AGTCATCAGA CTGGCGAGAA TCGGTAGAGT TCTTCGACTC
    ATTAGAGGAG CAAAGGGGAT TCGCACATTA CTTTTTGCCC TGATGATGTC TCTCCCCGCT
    CTCTTTAATA TCGGGCTTTT GCTTTTCTTA GTTATGTTCA TCTATTCAAT CTTTGGAATG
    TCCAATTTTG CCTATGTAAA GAAAGAATCT GGAATAGATG ACATATTTAA TTTTGAAACC
    TTTGGAAACA GCATAATATG CTTGTTCCAA ATCACCACGT CTGCTGGTTG GGATGGTCTT
    TTAAATCCAA TCCTAAATAG CGGCCCACCA GATTGTGACC CCCATCTGGA GAACTTTGGA
    ACAACAGTTA CTGGTGATTG TGGTAACCCA GCTATAGGTA TTGTGTTCTT CTGCAGCTAC
    ATCATAATAT CATTTTTAAT AGTTGTCAAT ATGTACATTG CCATTATCCT GGAGAACTTT
    AATGTTGCCA CAGAAGAGAG TGGTGAGCCA TTATGTGAGG ATGACTTTGA GATGTTTTAT
    GAAACTTGGG AGAAGTTTGA TCCAGATGCC ACTCAGTTTG TTGAATACAG TCGAATGTCA
    GACTTTGTAG ACACTTTGCA AGATCCTCTA CGGATTCCGA AACCTAACAA AATAAAACTA
    ATTACTTTGG ATCTCCCAAT GGTAACTGGA GATAAGATTC ACTGCTTGGA TATTCTGTTT
    GCACTAACTA AAGAGGTGTT GGGGGACTCA GGAGAAATGG ATGCATTAAA AGCCTCAATG
    GAAGAAAAGT TTATGATGGC AAATCCATCA AAAGTGTCTT ATGAACCAAT CACCACCACC
    CTTAAAAGAA AGCAGGAAGA AGTCTGTGTC ATCAAAATCC AGAGGGCCTT TAGGAGACAT
    CTATTGAGAC GTTCGGTGAA ACACGCTTCC TACATCTTCA GGCAAAGTAA TGATGAAGAG
    TCATCAGTGG AGGAACCTCC TGAAAAAGAA GGGTTGATAG CTGAAAGAAT TAATGCAGCC
    TACGGAAAAT ATGTAGAGGA AGGGCAAAAC GGCGGAACTC ATCGCCCAAG ATCAACAAAC
    AGAACTTCAC ACAGCAGTAT AGATTATGAT GACTCCAAAT CAAAAGCTCT TGCCTTCTTC
    CTGTTGGAAC TGACAGAGGG AACACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.