Scn4a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scn4a
Entrez Gene ID	110880
Full Name	sodium channel, voltage-gated, type IV, alpha
Synonyms	Nav1.4,SkM1,mH2
General protein information	Preferred Names sodium channel, voltage-gated, type IV, alpha Names sodium channel protein type 4 subunit alpha sodium channel alpha-subunit sodium channel protein skeletal muscle subunit alpha sodium channel protein type IV subunit alpha sodium channel, voltage-gated, type IV, alpha polypeptide voltage-gated sodium channel subunit alpha Nav1.4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 E1\|11 68.91 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314222.1	XP_017169711.1	sodium channel protein type 4 subunit alpha isoform X1
XM_006531987.2	XP_006532050.1	sodium channel protein type 4 subunit alpha isoform X2
XM_006531988.2	XP_006532051.1	sodium channel protein type 4 subunit alpha isoform X1
NM_133199.2	NP_573462.2	sodium channel protein type 4 subunit alpha
XM_030245472.1	XP_030101332.1	sodium channel protein type 4 subunit alpha isoform X1
XM_006531987.3	XP_006532050.1	sodium channel protein type 4 subunit alpha isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SCN4A	XP_853396.1
Rattus norvegicus (Norway rat)	Scn4a	NP_037310.1
Pan troglodytes (chimpanzee)	SCN4A	XP_003315741.1
	SCN4A	XP_001116451.1
Mus musculus (house mouse)	Scn4a	NP_573462.2
Xenopus tropicalis (tropical clawed frog)	LOC100492371	XP_002933087.2
Homo sapiens (human)	SCN4A	NP_000325.4
Bos taurus (cattle)	SCN4A	XP_003587508.1
Danio rerio (zebrafish)	scn4aa	NP_001034914.1
Gallus gallus (chicken)	SCN4A	XP_004948566.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Mice with an NaV1.4 sodium channel null allele have latent myasthenia, without susceptibility to periodic paralysis.
Wu F, Mi W, Fu Y, Struyk A, Cannon SC
Brain : a journal of neurology139(Pt 6)1688-99(2016 Jun)

Physiological basis for muscle stiffness and weakness in a knock-in M1592V mouse model of hyperkalemic periodic paralysis.
Khogali S, Lucas B, Ammar T, Dejong D, Barbalinardo M, Hayward LJ, Renaud JM
Physiological reports3(12)(2015 Dec)

Novel mutations in human and mouse SCN4A implicate AMPK in myotonia and periodic paralysis.
Corrochano S, M?nnikk? R, Joyce PI, McGoldrick P, Wettstein J, Lassi G, Raja Rayan DL, Blanco G, Quinn C, Liavas A, Lionikas A, Amior N, Dick J, Healy EG, Stewart M, Carter S, Hutchinson M, Bentley L, Fratta P, Cortese A, Cox R, Brown SD, Tucci V, Wackerhage H, Amato AA, Greensmith L, Koltzenburg M, Hanna MG, Acevedo-Arozena A
Brain : a journal of neurology137(Pt 12)3171-85(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NaV1.4 null mice have latent myasthenia and a right shift of the force-stimulus relation, without evidence of periodic paralysis. Sodium current density was half that of wild-type muscle and no compensation by retained expression of the foetal NaV1.5 isoform was detected. Mice null for NaV1.4 did not survive beyond the second postnatal day.
Title: Mice with an NaV1.4 sodium channel null allele have latent myasthenia, without susceptibility to periodic paralysis.

In-depth characterization of draggen mice uncovered novel systemic metabolic abnormalities in Scn4a mouse models and provided novel insights into disease mechanisms
Title: Novel mutations in human and mouse SCN4A implicate AMPK in myotonia and periodic paralysis.

HyperKPP phenotype does not depend solely on the NaV1.4 content.
Title: Contractile abnormalities of mouse muscles expressing hyperkalemic periodic paralysis mutant NaV1.4 channels do not correlate with Na+ influx or channel content.

Homozygous R669H mice had a robust hypokalemic periodic paralysis phenotype, with transient loss of muscle excitability and weakness in low-K+ challenge, insensitivity to high-K+ challenge, dominant inheritance, and absence of myotonia.
Title: A sodium channel knockin mutant (NaV1.4-R669H) mouse model of hypokalemic periodic paralysis.

The skeletal muscle isoform of Na(v)1.4, which represents over 90% of voltage-gated sodium channels in muscle, plays an important role in development of abnormally high Na(+) concentrations found in muscle from mdx mice.
Title: Nav1.4 deregulation in dystrophic skeletal muscle leads to Na+ overload and enhanced cell death.

The following Scn4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scn4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu77704	XM_017314222.1 Latest version!	Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu77704	XM_030245472.1 Latest version!	Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu09887	NM_133199.2 Latest version!	Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$951.30 $1359.00
OMu09887	XM_006531987.3 Latest version!	Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu09887	XM_006531988.2 Latest version!	Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$951.30 $1359.00
View Old Accession Versions >>
OMu09887	XM_006531987.2	Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scn4a ( XM_017314222.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169711.1 Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77704
Clone ID Related Accession (Same CDS sequence)	XM_017314222.1 , XM_030245472.1
Accession Version	XM_017314222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sodium channel protein type 4 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CATCTCTGCC CACCCTGGTC CCCCCTGGTC CCCACTGCCT GCGCCCCTTC 
ACCCCAGAGT CCCTGGCAGC CATAGAACAG CGGGCAATGG AGGAGGAAGC CCGGCTGCAG 
CGGAACAAGC AAATGGAGAT TGAAGAGCCT GAGCGGAAGC CACGCAGTGA CCTGGAAGCT 
GGCAAGAACC TCCCACTCAT CTATGGGGAC CCCCCACCTG AAGTCATTGG CGTCCCCCTG 
GAGGACCTGG ACCCTTACTA CAGTGACAAG AAGACCTTCA TTGTGCTCAA CAAAGGAAAG 
GCCATCTTCC GATTCTCTGC CACCCCTGCC CTCTACATGC TGAGCCCCTT CAGCATCGTC 
CGGCGGGTGG CTATCAAGGT GCTCATCCAC GCGCTGTTCA GCATGTTCAT CATGATCACC 
ATCTTGACCA ACTGCGTGTT CATGACCATG AGCAACCCGC CTTCTTGGTC CAAGGACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTT GAGTCCCTCA TTAAGATGCT GGCCCGAGGC 
TTTTGCATTG ATGACTTCAC ATTCCTCCGA GACCCCTGGA ACTGGCTGGA CTTCAGTGTC 
ATCACAATGG CGTACGTGAC AGAGTTTGTG GACTTGGGCA ACATCTCAGC CCTGAGGACC 
TTCCGTGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACGATCGTG 
GGAGCCCTGA TCCAGTCTGT GAAAAAGCTC TCGGATGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTTTTCATGG GAAACCTGCG TCAGAAGTGC 
GTGCGTTGGC CCCCGCCCAT GAATGACACC AACACCACGT GGTATGGTAA TGATACGTGG 
TACGGCAATG ACACCTGGTA CGGCAATGAC ACCTGGTATG GCAATGACAC TTGGAACAGC 
CAGGAGAGCT GGGTCAGCAA CTCTACCTTT GACTGGGAGG CCTACATCAA TGACGAAGGG 
AACTTCTATT TCTTGGAGGG CTCCAATGAT GCCCTGCTCT GTGGGAATAG CAGTGATGCC 
GGGCACTGCC CTGAGGGCTA CGAATGCATG AAGGCTGGGA GGAACCCCAA CTATGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACGCAGGAC 
TACTGGGAGA ACCTTTTCCA GCTGACCCTT CGAGCCGCTG GCAAGACCTA CATGATCTTC 
TTTGTGGTCA TCATCTTCCT GGGTTCCTTC TACCTCATCA ATCTGATCCT GGCGGTGGTG 
GCCATGGCAT ATGCTGAGCA GAATGAGGCT ACCCTGGCCG AAGACCAGGA GAAAGAGGAG 
GAGTTCCAAC AGATGCTTGA AAAATTCAAA AAACATCAGG AGGAACTGGA AAAGGCGAAG 
GCTGCCCAGG CTCTGGAAGG TGGAGAGGAG GCAGATGGGG ACCCAACCCA CAGCAAAGAC 
TGCAATGGTA GCCTGGATAC ATCCGGGGAG AAGGGGCCCC CAAGGCCAAG CTGCAGCGCA 
GAGAGTGCCA TCTCAGATGC TATGGAGGAG CTGGAAGAGG CCCATCAGAA GTGCCCCCCA 
TGGTGGTACA AGTGTGCACA CAAAGTGCTC ATCTGGAACT GCTGTGCCCC GTGGGTGAAG 
TTCAAGCATA TTATCCTCCT GATTGTCATG GACCCCTTTG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCACT ACCCCATGAC TGAGCACTTT 
GACAACGTGC TCTCTGTGGG CAACTTGGTC TTCACGGGCA TCTTCACTGC AGAGATGGTG 
CTGAAGTTGA TTGCTATGGA CCCCTACGAA TATTTCCAAC AGGGCTGGAA CATCTTCGAC 
AGCTTCATCG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAACGTGCA GGGCCTATCA 
GTGCTCCGTT CTTTCCGTCT GCTGCGTGTC TTCAAGCTGG CCAAGTCATG GCCAACACTC 
AACATGCTCA TCAAAATCAT TGGCAACTCA GTGGGTGCGC TGGGCAACCT GACCCTGGTG 
TTGGCCATCA TCGTCTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAT 
AAGGAATGCG TGTGCAAGAT TGCCTCAGAC TGCAGCCTGC CCCGCTGGCA CATGCACGAT 
TTCTTCCACT CCTTCCTCAT CGTCTTCCGC ATCCTCTGTG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAAGTGGC CGGCCAGGCC ATGTGCCTCA CCGTCTTCCT CATGGTCATG 
GTCATTGGCA ACCTGGTGGT CCTGAATCTG TTCCTGGCTC TCCTGTTGAG TTCCTTCAGC 
GCTGACAGTC TGGCAGCGTC AGATGAAGAT GGTGAGATGA ACAACCTACA GATCGCCATC 
GGGCGTATCA AGTGGGGCAT CGCCTTTGCC AAAACCTTCC TCTTGGGGCT GTTGCATGGC 
AAGATCCTGA GCCTCAAAGA CATAATGCTC AGTCTCGGGG AGCCTGGGGG TGCTGGGGAA 
AACGGGGAGA GCCCTCCCGA GGATGAGAAG AAGGAACCAC CTCCGGAAGA TGGGAACAAG 
GAGCTGAAAG ACAATCATAT CCTGAACCAC GTGGGCCTGA CTGATGGACC CCGCTCCAGC 
ATCGAGATGG ACCACCTTAA CTTCATCAAC AACCCCTACC TCACTATCCA TGTGCCCATT 
GCCTCCGAGG AGTCCGACTT GGAAATGCCC ACAGAGGAGG AGACTGACAC CTTCTCGGAG 
CCTGAGGACA TCAAGAAGCC ACTACAGCCT CTGTACGACG GGAACTCCTC CGTCTGCAGC 
ACAGCTGACT ACAAGCCCCC CGAAGAGGAC CCTGAGGAGC AGGCTGAGGA GAACCCTGAG 
GGGGAGCTAC CTGAGGAGTG TTTCACAGAG GCCTGTGTGA AGCGCTGCCC CTGCCTCTAT 
GTGGACATCT CCCAGGGCCG CGGGAAAATG TGGTGGACAC TCCGCAGGGC CTGCTTCAAG 
ATTGTTGAGC ACAACTGGTT TGAGACCTTC ATCGTCTTCA TGATCTTGCT CAGCAGCGGG 
GCCCTGGCCT TCGAGGACAT CTACATTGAA CAGCGGCGAG TCATCCAAAC CATCCTGGAA 
TATGCTGACA AGGTCTTCAC CTACATCTTC ATCCTGGAGA TGCTGCTAAA GTGGGTGGCC 
TACGGCTTCA AGGTGTACTT CACCAACGCC TGGTGCTGGC TCGACTTCCT CATTGTGGAT 
GTCTCCATCA TCAGTCTGGT GGCTAACTGG CTGGGCTACT CTGAGCTGGG GCCCATCAAA 
TCCCTGCGCA CGCTCCGGGC CCTGCGACCT CTGAGGGCAC TGTCCCGATT TGAAGGCATG 
AGGGTGGTGG TGAACGCCCT TCTGGGAGCC ATCCCTTCCA TCATGAACGT GCTCCTTGTC 
TGCCTCATCT TCTGGCTCAT CTTCAGCATC ATGGGGGTCA ACTTGTTTGC TGGAAAGTTC 
TACTACTGCA TCAACACAAC CACCTCCGAG AGATTCGACA TCTCTGTGGT CAACAACAAG 
TCCGAGTGTG AGAGCCTCAT GTACACCGGC CAGGTCCGCT GGATGAACGT CAAGGTCAAC 
TATGACAACG TGGGTCTCGG CTACCTCTCT CTTCTTCAGG TGGCCACTTT CAAGGGTTGG 
ATGGACATCA TGTATGCAGC TGTGGACTCC CGGGAGAAAG AGGAGCAGCC AGACTACGAG 
GTGAACCTCT ACATGTACCT TTACTTCGTC ATCTTTATCA TCTTCGGCTC CTTCTTCACC 
CTCAATCTCT TCATTGGCGT CATCATTGAC AACTTCAACC AACAGAAGAA GAAGATGAGT 
ATCTGGCCCT CTCCAAAGGC TCCCTGCCCC CATCCATCTA TTCTTTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169711.1
CDS	549..4499
Translation	MASSSLPTLVPPGPHCLRPFTPESLAAIEQRAMEEEARLQRNKQMEIEEPERKPRSDLEAGKNLPLIYGDPPPEVIGVPLEDLDPYYSDKKTFIVLNKGKAIFRFSATPALYMLSPFSIVRRVAIKVLIHALFSMFIMITILTNCVFMTMSNPPSWSKDVEYTFTGIYTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVITMAYVTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPMNDTNTTWYGNDTWYGNDTWYGNDTWYGNDTWNSQESWVSNSTFDWEAYINDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECMKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKHQEELEKAKAAQALEGGEEADGDPTHSKDCNGSLDTSGEKGPPRPSCSAESAISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWVKFKHIILLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLSVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSFIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCSLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIGRIKWGIAFAKTFLLGLLHGKILSLKDIMLSLGEPGGAGENGESPPEDEKKEPPPEDGNKELKDNHILNHVGLTDGPRSSIEMDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDIKKPLQPLYDGNSSVCSTADYKPPEEDPEEQAEENPEGELPEECFTEACVKRCPCLYVDISQGRGKMWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRRVIQTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDISVVNNKSECESLMYTGQVRWMNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPDYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKMSIWPSPKAPCPHPSILS

Target ORF information:

RefSeq Version	XM_017314222.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314222.1

ORF Insert Sequence:

ATGGCCAGCT CATCTCTGCC CACCCTGGTC CCCCCTGGTC CCCACTGCCT GCGCCCCTTC 
ACCCCAGAGT CCCTGGCAGC CATAGAACAG CGGGCAATGG AGGAGGAAGC CCGGCTGCAG 
CGGAACAAGC AAATGGAGAT TGAAGAGCCT GAGCGGAAGC CACGCAGTGA CCTGGAAGCT 
GGCAAGAACC TCCCACTCAT CTATGGGGAC CCCCCACCTG AAGTCATTGG CGTCCCCCTG 
GAGGACCTGG ACCCTTACTA CAGTGACAAG AAGACCTTCA TTGTGCTCAA CAAAGGAAAG 
GCCATCTTCC GATTCTCTGC CACCCCTGCC CTCTACATGC TGAGCCCCTT CAGCATCGTC 
CGGCGGGTGG CTATCAAGGT GCTCATCCAC GCGCTGTTCA GCATGTTCAT CATGATCACC 
ATCTTGACCA ACTGCGTGTT CATGACCATG AGCAACCCGC CTTCTTGGTC CAAGGACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTT GAGTCCCTCA TTAAGATGCT GGCCCGAGGC 
TTTTGCATTG ATGACTTCAC ATTCCTCCGA GACCCCTGGA ACTGGCTGGA CTTCAGTGTC 
ATCACAATGG CGTACGTGAC AGAGTTTGTG GACTTGGGCA ACATCTCAGC CCTGAGGACC 
TTCCGTGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACGATCGTG 
GGAGCCCTGA TCCAGTCTGT GAAAAAGCTC TCGGATGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTTTTCATGG GAAACCTGCG TCAGAAGTGC 
GTGCGTTGGC CCCCGCCCAT GAATGACACC AACACCACGT GGTATGGTAA TGATACGTGG 
TACGGCAATG ACACCTGGTA CGGCAATGAC ACCTGGTATG GCAATGACAC TTGGAACAGC 
CAGGAGAGCT GGGTCAGCAA CTCTACCTTT GACTGGGAGG CCTACATCAA TGACGAAGGG 
AACTTCTATT TCTTGGAGGG CTCCAATGAT GCCCTGCTCT GTGGGAATAG CAGTGATGCC 
GGGCACTGCC CTGAGGGCTA CGAATGCATG AAGGCTGGGA GGAACCCCAA CTATGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACGCAGGAC 
TACTGGGAGA ACCTTTTCCA GCTGACCCTT CGAGCCGCTG GCAAGACCTA CATGATCTTC 
TTTGTGGTCA TCATCTTCCT GGGTTCCTTC TACCTCATCA ATCTGATCCT GGCGGTGGTG 
GCCATGGCAT ATGCTGAGCA GAATGAGGCT ACCCTGGCCG AAGACCAGGA GAAAGAGGAG 
GAGTTCCAAC AGATGCTTGA AAAATTCAAA AAACATCAGG AGGAACTGGA AAAGGCGAAG 
GCTGCCCAGG CTCTGGAAGG TGGAGAGGAG GCAGATGGGG ACCCAACCCA CAGCAAAGAC 
TGCAATGGTA GCCTGGATAC ATCCGGGGAG AAGGGGCCCC CAAGGCCAAG CTGCAGCGCA 
GAGAGTGCCA TCTCAGATGC TATGGAGGAG CTGGAAGAGG CCCATCAGAA GTGCCCCCCA 
TGGTGGTACA AGTGTGCACA CAAAGTGCTC ATCTGGAACT GCTGTGCCCC GTGGGTGAAG 
TTCAAGCATA TTATCCTCCT GATTGTCATG GACCCCTTTG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCACT ACCCCATGAC TGAGCACTTT 
GACAACGTGC TCTCTGTGGG CAACTTGGTC TTCACGGGCA TCTTCACTGC AGAGATGGTG 
CTGAAGTTGA TTGCTATGGA CCCCTACGAA TATTTCCAAC AGGGCTGGAA CATCTTCGAC 
AGCTTCATCG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAACGTGCA GGGCCTATCA 
GTGCTCCGTT CTTTCCGTCT GCTGCGTGTC TTCAAGCTGG CCAAGTCATG GCCAACACTC 
AACATGCTCA TCAAAATCAT TGGCAACTCA GTGGGTGCGC TGGGCAACCT GACCCTGGTG 
TTGGCCATCA TCGTCTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAT 
AAGGAATGCG TGTGCAAGAT TGCCTCAGAC TGCAGCCTGC CCCGCTGGCA CATGCACGAT 
TTCTTCCACT CCTTCCTCAT CGTCTTCCGC ATCCTCTGTG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAAGTGGC CGGCCAGGCC ATGTGCCTCA CCGTCTTCCT CATGGTCATG 
GTCATTGGCA ACCTGGTGGT CCTGAATCTG TTCCTGGCTC TCCTGTTGAG TTCCTTCAGC 
GCTGACAGTC TGGCAGCGTC AGATGAAGAT GGTGAGATGA ACAACCTACA GATCGCCATC 
GGGCGTATCA AGTGGGGCAT CGCCTTTGCC AAAACCTTCC TCTTGGGGCT GTTGCATGGC 
AAGATCCTGA GCCTCAAAGA CATAATGCTC AGTCTCGGGG AGCCTGGGGG TGCTGGGGAA 
AACGGGGAGA GCCCTCCCGA GGATGAGAAG AAGGAACCAC CTCCGGAAGA TGGGAACAAG 
GAGCTGAAAG ACAATCATAT CCTGAACCAC GTGGGCCTGA CTGATGGACC CCGCTCCAGC 
ATCGAGATGG ACCACCTTAA CTTCATCAAC AACCCCTACC TCACTATCCA TGTGCCCATT 
GCCTCCGAGG AGTCCGACTT GGAAATGCCC ACAGAGGAGG AGACTGACAC CTTCTCGGAG 
CCTGAGGACA TCAAGAAGCC ACTACAGCCT CTGTACGACG GGAACTCCTC CGTCTGCAGC 
ACAGCTGACT ACAAGCCCCC CGAAGAGGAC CCTGAGGAGC AGGCTGAGGA GAACCCTGAG 
GGGGAGCTAC CTGAGGAGTG TTTCACAGAG GCCTGTGTGA AGCGCTGCCC CTGCCTCTAT 
GTGGACATCT CCCAGGGCCG CGGGAAAATG TGGTGGACAC TCCGCAGGGC CTGCTTCAAG 
ATTGTTGAGC ACAACTGGTT TGAGACCTTC ATCGTCTTCA TGATCTTGCT CAGCAGCGGG 
GCCCTGGCCT TCGAGGACAT CTACATTGAA CAGCGGCGAG TCATCCAAAC CATCCTGGAA 
TATGCTGACA AGGTCTTCAC CTACATCTTC ATCCTGGAGA TGCTGCTAAA GTGGGTGGCC 
TACGGCTTCA AGGTGTACTT CACCAACGCC TGGTGCTGGC TCGACTTCCT CATTGTGGAT 
GTCTCCATCA TCAGTCTGGT GGCTAACTGG CTGGGCTACT CTGAGCTGGG GCCCATCAAA 
TCCCTGCGCA CGCTCCGGGC CCTGCGACCT CTGAGGGCAC TGTCCCGATT TGAAGGCATG 
AGGGTGGTGG TGAACGCCCT TCTGGGAGCC ATCCCTTCCA TCATGAACGT GCTCCTTGTC 
TGCCTCATCT TCTGGCTCAT CTTCAGCATC ATGGGGGTCA ACTTGTTTGC TGGAAAGTTC 
TACTACTGCA TCAACACAAC CACCTCCGAG AGATTCGACA TCTCTGTGGT CAACAACAAG 
TCCGAGTGTG AGAGCCTCAT GTACACCGGC CAGGTCCGCT GGATGAACGT CAAGGTCAAC 
TATGACAACG TGGGTCTCGG CTACCTCTCT CTTCTTCAGG TGGCCACTTT CAAGGGTTGG 
ATGGACATCA TGTATGCAGC TGTGGACTCC CGGGAGAAAG AGGAGCAGCC AGACTACGAG 
GTGAACCTCT ACATGTACCT TTACTTCGTC ATCTTTATCA TCTTCGGCTC CTTCTTCACC 
CTCAATCTCT TCATTGGCGT CATCATTGAC AACTTCAACC AACAGAAGAA GAAGATGAGT 
ATCTGGCCCT CTCCAAAGGC TCCCTGCCCC CATCCATCTA TTCTTTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn4a ( XM_030245472.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101332.1 Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X1, mRNA.

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CloneID	OMu77704
Clone ID Related Accession (Same CDS sequence)	XM_017314222.1 , XM_030245472.1
Accession Version	XM_030245472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sodium channel protein type 4 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CATCTCTGCC CACCCTGGTC CCCCCTGGTC CCCACTGCCT GCGCCCCTTC 
ACCCCAGAGT CCCTGGCAGC CATAGAACAG CGGGCAATGG AGGAGGAAGC CCGGCTGCAG 
CGGAACAAGC AAATGGAGAT TGAAGAGCCT GAGCGGAAGC CACGCAGTGA CCTGGAAGCT 
GGCAAGAACC TCCCACTCAT CTATGGGGAC CCCCCACCTG AAGTCATTGG CGTCCCCCTG 
GAGGACCTGG ACCCTTACTA CAGTGACAAG AAGACCTTCA TTGTGCTCAA CAAAGGAAAG 
GCCATCTTCC GATTCTCTGC CACCCCTGCC CTCTACATGC TGAGCCCCTT CAGCATCGTC 
CGGCGGGTGG CTATCAAGGT GCTCATCCAC GCGCTGTTCA GCATGTTCAT CATGATCACC 
ATCTTGACCA ACTGCGTGTT CATGACCATG AGCAACCCGC CTTCTTGGTC CAAGGACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTT GAGTCCCTCA TTAAGATGCT GGCCCGAGGC 
TTTTGCATTG ATGACTTCAC ATTCCTCCGA GACCCCTGGA ACTGGCTGGA CTTCAGTGTC 
ATCACAATGG CGTACGTGAC AGAGTTTGTG GACTTGGGCA ACATCTCAGC CCTGAGGACC 
TTCCGTGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACGATCGTG 
GGAGCCCTGA TCCAGTCTGT GAAAAAGCTC TCGGATGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTTTTCATGG GAAACCTGCG TCAGAAGTGC 
GTGCGTTGGC CCCCGCCCAT GAATGACACC AACACCACGT GGTATGGTAA TGATACGTGG 
TACGGCAATG ACACCTGGTA CGGCAATGAC ACCTGGTATG GCAATGACAC TTGGAACAGC 
CAGGAGAGCT GGGTCAGCAA CTCTACCTTT GACTGGGAGG CCTACATCAA TGACGAAGGG 
AACTTCTATT TCTTGGAGGG CTCCAATGAT GCCCTGCTCT GTGGGAATAG CAGTGATGCC 
GGGCACTGCC CTGAGGGCTA CGAATGCATG AAGGCTGGGA GGAACCCCAA CTATGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACGCAGGAC 
TACTGGGAGA ACCTTTTCCA GCTGACCCTT CGAGCCGCTG GCAAGACCTA CATGATCTTC 
TTTGTGGTCA TCATCTTCCT GGGTTCCTTC TACCTCATCA ATCTGATCCT GGCGGTGGTG 
GCCATGGCAT ATGCTGAGCA GAATGAGGCT ACCCTGGCCG AAGACCAGGA GAAAGAGGAG 
GAGTTCCAAC AGATGCTTGA AAAATTCAAA AAACATCAGG AGGAACTGGA AAAGGCGAAG 
GCTGCCCAGG CTCTGGAAGG TGGAGAGGAG GCAGATGGGG ACCCAACCCA CAGCAAAGAC 
TGCAATGGTA GCCTGGATAC ATCCGGGGAG AAGGGGCCCC CAAGGCCAAG CTGCAGCGCA 
GAGAGTGCCA TCTCAGATGC TATGGAGGAG CTGGAAGAGG CCCATCAGAA GTGCCCCCCA 
TGGTGGTACA AGTGTGCACA CAAAGTGCTC ATCTGGAACT GCTGTGCCCC GTGGGTGAAG 
TTCAAGCATA TTATCCTCCT GATTGTCATG GACCCCTTTG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCACT ACCCCATGAC TGAGCACTTT 
GACAACGTGC TCTCTGTGGG CAACTTGGTC TTCACGGGCA TCTTCACTGC AGAGATGGTG 
CTGAAGTTGA TTGCTATGGA CCCCTACGAA TATTTCCAAC AGGGCTGGAA CATCTTCGAC 
AGCTTCATCG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAACGTGCA GGGCCTATCA 
GTGCTCCGTT CTTTCCGTCT GCTGCGTGTC TTCAAGCTGG CCAAGTCATG GCCAACACTC 
AACATGCTCA TCAAAATCAT TGGCAACTCA GTGGGTGCGC TGGGCAACCT GACCCTGGTG 
TTGGCCATCA TCGTCTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAT 
AAGGAATGCG TGTGCAAGAT TGCCTCAGAC TGCAGCCTGC CCCGCTGGCA CATGCACGAT 
TTCTTCCACT CCTTCCTCAT CGTCTTCCGC ATCCTCTGTG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAAGTGGC CGGCCAGGCC ATGTGCCTCA CCGTCTTCCT CATGGTCATG 
GTCATTGGCA ACCTGGTGGT CCTGAATCTG TTCCTGGCTC TCCTGTTGAG TTCCTTCAGC 
GCTGACAGTC TGGCAGCGTC AGATGAAGAT GGTGAGATGA ACAACCTACA GATCGCCATC 
GGGCGTATCA AGTGGGGCAT CGCCTTTGCC AAAACCTTCC TCTTGGGGCT GTTGCATGGC 
AAGATCCTGA GCCTCAAAGA CATAATGCTC AGTCTCGGGG AGCCTGGGGG TGCTGGGGAA 
AACGGGGAGA GCCCTCCCGA GGATGAGAAG AAGGAACCAC CTCCGGAAGA TGGGAACAAG 
GAGCTGAAAG ACAATCATAT CCTGAACCAC GTGGGCCTGA CTGATGGACC CCGCTCCAGC 
ATCGAGATGG ACCACCTTAA CTTCATCAAC AACCCCTACC TCACTATCCA TGTGCCCATT 
GCCTCCGAGG AGTCCGACTT GGAAATGCCC ACAGAGGAGG AGACTGACAC CTTCTCGGAG 
CCTGAGGACA TCAAGAAGCC ACTACAGCCT CTGTACGACG GGAACTCCTC CGTCTGCAGC 
ACAGCTGACT ACAAGCCCCC CGAAGAGGAC CCTGAGGAGC AGGCTGAGGA GAACCCTGAG 
GGGGAGCTAC CTGAGGAGTG TTTCACAGAG GCCTGTGTGA AGCGCTGCCC CTGCCTCTAT 
GTGGACATCT CCCAGGGCCG CGGGAAAATG TGGTGGACAC TCCGCAGGGC CTGCTTCAAG 
ATTGTTGAGC ACAACTGGTT TGAGACCTTC ATCGTCTTCA TGATCTTGCT CAGCAGCGGG 
GCCCTGGCCT TCGAGGACAT CTACATTGAA CAGCGGCGAG TCATCCAAAC CATCCTGGAA 
TATGCTGACA AGGTCTTCAC CTACATCTTC ATCCTGGAGA TGCTGCTAAA GTGGGTGGCC 
TACGGCTTCA AGGTGTACTT CACCAACGCC TGGTGCTGGC TCGACTTCCT CATTGTGGAT 
GTCTCCATCA TCAGTCTGGT GGCTAACTGG CTGGGCTACT CTGAGCTGGG GCCCATCAAA 
TCCCTGCGCA CGCTCCGGGC CCTGCGACCT CTGAGGGCAC TGTCCCGATT TGAAGGCATG 
AGGGTGGTGG TGAACGCCCT TCTGGGAGCC ATCCCTTCCA TCATGAACGT GCTCCTTGTC 
TGCCTCATCT TCTGGCTCAT CTTCAGCATC ATGGGGGTCA ACTTGTTTGC TGGAAAGTTC 
TACTACTGCA TCAACACAAC CACCTCCGAG AGATTCGACA TCTCTGTGGT CAACAACAAG 
TCCGAGTGTG AGAGCCTCAT GTACACCGGC CAGGTCCGCT GGATGAACGT CAAGGTCAAC 
TATGACAACG TGGGTCTCGG CTACCTCTCT CTTCTTCAGG TGGCCACTTT CAAGGGTTGG 
ATGGACATCA TGTATGCAGC TGTGGACTCC CGGGAGAAAG AGGAGCAGCC AGACTACGAG 
GTGAACCTCT ACATGTACCT TTACTTCGTC ATCTTTATCA TCTTCGGCTC CTTCTTCACC 
CTCAATCTCT TCATTGGCGT CATCATTGAC AACTTCAACC AACAGAAGAA GAAGATGAGT 
ATCTGGCCCT CTCCAAAGGC TCCCTGCCCC CATCCATCTA TTCTTTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101332.1
CDS	424..4374
Translation	MASSSLPTLVPPGPHCLRPFTPESLAAIEQRAMEEEARLQRNKQMEIEEPERKPRSDLEAGKNLPLIYGDPPPEVIGVPLEDLDPYYSDKKTFIVLNKGKAIFRFSATPALYMLSPFSIVRRVAIKVLIHALFSMFIMITILTNCVFMTMSNPPSWSKDVEYTFTGIYTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVITMAYVTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPMNDTNTTWYGNDTWYGNDTWYGNDTWYGNDTWNSQESWVSNSTFDWEAYINDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECMKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKHQEELEKAKAAQALEGGEEADGDPTHSKDCNGSLDTSGEKGPPRPSCSAESAISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWVKFKHIILLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLSVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSFIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCSLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIGRIKWGIAFAKTFLLGLLHGKILSLKDIMLSLGEPGGAGENGESPPEDEKKEPPPEDGNKELKDNHILNHVGLTDGPRSSIEMDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDIKKPLQPLYDGNSSVCSTADYKPPEEDPEEQAEENPEGELPEECFTEACVKRCPCLYVDISQGRGKMWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRRVIQTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDISVVNNKSECESLMYTGQVRWMNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPDYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKMSIWPSPKAPCPHPSILS

Target ORF information:

RefSeq Version	XM_030245472.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245472.1

ORF Insert Sequence:

ATGGCCAGCT CATCTCTGCC CACCCTGGTC CCCCCTGGTC CCCACTGCCT GCGCCCCTTC 
ACCCCAGAGT CCCTGGCAGC CATAGAACAG CGGGCAATGG AGGAGGAAGC CCGGCTGCAG 
CGGAACAAGC AAATGGAGAT TGAAGAGCCT GAGCGGAAGC CACGCAGTGA CCTGGAAGCT 
GGCAAGAACC TCCCACTCAT CTATGGGGAC CCCCCACCTG AAGTCATTGG CGTCCCCCTG 
GAGGACCTGG ACCCTTACTA CAGTGACAAG AAGACCTTCA TTGTGCTCAA CAAAGGAAAG 
GCCATCTTCC GATTCTCTGC CACCCCTGCC CTCTACATGC TGAGCCCCTT CAGCATCGTC 
CGGCGGGTGG CTATCAAGGT GCTCATCCAC GCGCTGTTCA GCATGTTCAT CATGATCACC 
ATCTTGACCA ACTGCGTGTT CATGACCATG AGCAACCCGC CTTCTTGGTC CAAGGACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTT GAGTCCCTCA TTAAGATGCT GGCCCGAGGC 
TTTTGCATTG ATGACTTCAC ATTCCTCCGA GACCCCTGGA ACTGGCTGGA CTTCAGTGTC 
ATCACAATGG CGTACGTGAC AGAGTTTGTG GACTTGGGCA ACATCTCAGC CCTGAGGACC 
TTCCGTGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACGATCGTG 
GGAGCCCTGA TCCAGTCTGT GAAAAAGCTC TCGGATGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTTTTCATGG GAAACCTGCG TCAGAAGTGC 
GTGCGTTGGC CCCCGCCCAT GAATGACACC AACACCACGT GGTATGGTAA TGATACGTGG 
TACGGCAATG ACACCTGGTA CGGCAATGAC ACCTGGTATG GCAATGACAC TTGGAACAGC 
CAGGAGAGCT GGGTCAGCAA CTCTACCTTT GACTGGGAGG CCTACATCAA TGACGAAGGG 
AACTTCTATT TCTTGGAGGG CTCCAATGAT GCCCTGCTCT GTGGGAATAG CAGTGATGCC 
GGGCACTGCC CTGAGGGCTA CGAATGCATG AAGGCTGGGA GGAACCCCAA CTATGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACGCAGGAC 
TACTGGGAGA ACCTTTTCCA GCTGACCCTT CGAGCCGCTG GCAAGACCTA CATGATCTTC 
TTTGTGGTCA TCATCTTCCT GGGTTCCTTC TACCTCATCA ATCTGATCCT GGCGGTGGTG 
GCCATGGCAT ATGCTGAGCA GAATGAGGCT ACCCTGGCCG AAGACCAGGA GAAAGAGGAG 
GAGTTCCAAC AGATGCTTGA AAAATTCAAA AAACATCAGG AGGAACTGGA AAAGGCGAAG 
GCTGCCCAGG CTCTGGAAGG TGGAGAGGAG GCAGATGGGG ACCCAACCCA CAGCAAAGAC 
TGCAATGGTA GCCTGGATAC ATCCGGGGAG AAGGGGCCCC CAAGGCCAAG CTGCAGCGCA 
GAGAGTGCCA TCTCAGATGC TATGGAGGAG CTGGAAGAGG CCCATCAGAA GTGCCCCCCA 
TGGTGGTACA AGTGTGCACA CAAAGTGCTC ATCTGGAACT GCTGTGCCCC GTGGGTGAAG 
TTCAAGCATA TTATCCTCCT GATTGTCATG GACCCCTTTG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCACT ACCCCATGAC TGAGCACTTT 
GACAACGTGC TCTCTGTGGG CAACTTGGTC TTCACGGGCA TCTTCACTGC AGAGATGGTG 
CTGAAGTTGA TTGCTATGGA CCCCTACGAA TATTTCCAAC AGGGCTGGAA CATCTTCGAC 
AGCTTCATCG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAACGTGCA GGGCCTATCA 
GTGCTCCGTT CTTTCCGTCT GCTGCGTGTC TTCAAGCTGG CCAAGTCATG GCCAACACTC 
AACATGCTCA TCAAAATCAT TGGCAACTCA GTGGGTGCGC TGGGCAACCT GACCCTGGTG 
TTGGCCATCA TCGTCTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAT 
AAGGAATGCG TGTGCAAGAT TGCCTCAGAC TGCAGCCTGC CCCGCTGGCA CATGCACGAT 
TTCTTCCACT CCTTCCTCAT CGTCTTCCGC ATCCTCTGTG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAAGTGGC CGGCCAGGCC ATGTGCCTCA CCGTCTTCCT CATGGTCATG 
GTCATTGGCA ACCTGGTGGT CCTGAATCTG TTCCTGGCTC TCCTGTTGAG TTCCTTCAGC 
GCTGACAGTC TGGCAGCGTC AGATGAAGAT GGTGAGATGA ACAACCTACA GATCGCCATC 
GGGCGTATCA AGTGGGGCAT CGCCTTTGCC AAAACCTTCC TCTTGGGGCT GTTGCATGGC 
AAGATCCTGA GCCTCAAAGA CATAATGCTC AGTCTCGGGG AGCCTGGGGG TGCTGGGGAA 
AACGGGGAGA GCCCTCCCGA GGATGAGAAG AAGGAACCAC CTCCGGAAGA TGGGAACAAG 
GAGCTGAAAG ACAATCATAT CCTGAACCAC GTGGGCCTGA CTGATGGACC CCGCTCCAGC 
ATCGAGATGG ACCACCTTAA CTTCATCAAC AACCCCTACC TCACTATCCA TGTGCCCATT 
GCCTCCGAGG AGTCCGACTT GGAAATGCCC ACAGAGGAGG AGACTGACAC CTTCTCGGAG 
CCTGAGGACA TCAAGAAGCC ACTACAGCCT CTGTACGACG GGAACTCCTC CGTCTGCAGC 
ACAGCTGACT ACAAGCCCCC CGAAGAGGAC CCTGAGGAGC AGGCTGAGGA GAACCCTGAG 
GGGGAGCTAC CTGAGGAGTG TTTCACAGAG GCCTGTGTGA AGCGCTGCCC CTGCCTCTAT 
GTGGACATCT CCCAGGGCCG CGGGAAAATG TGGTGGACAC TCCGCAGGGC CTGCTTCAAG 
ATTGTTGAGC ACAACTGGTT TGAGACCTTC ATCGTCTTCA TGATCTTGCT CAGCAGCGGG 
GCCCTGGCCT TCGAGGACAT CTACATTGAA CAGCGGCGAG TCATCCAAAC CATCCTGGAA 
TATGCTGACA AGGTCTTCAC CTACATCTTC ATCCTGGAGA TGCTGCTAAA GTGGGTGGCC 
TACGGCTTCA AGGTGTACTT CACCAACGCC TGGTGCTGGC TCGACTTCCT CATTGTGGAT 
GTCTCCATCA TCAGTCTGGT GGCTAACTGG CTGGGCTACT CTGAGCTGGG GCCCATCAAA 
TCCCTGCGCA CGCTCCGGGC CCTGCGACCT CTGAGGGCAC TGTCCCGATT TGAAGGCATG 
AGGGTGGTGG TGAACGCCCT TCTGGGAGCC ATCCCTTCCA TCATGAACGT GCTCCTTGTC 
TGCCTCATCT TCTGGCTCAT CTTCAGCATC ATGGGGGTCA ACTTGTTTGC TGGAAAGTTC 
TACTACTGCA TCAACACAAC CACCTCCGAG AGATTCGACA TCTCTGTGGT CAACAACAAG 
TCCGAGTGTG AGAGCCTCAT GTACACCGGC CAGGTCCGCT GGATGAACGT CAAGGTCAAC 
TATGACAACG TGGGTCTCGG CTACCTCTCT CTTCTTCAGG TGGCCACTTT CAAGGGTTGG 
ATGGACATCA TGTATGCAGC TGTGGACTCC CGGGAGAAAG AGGAGCAGCC AGACTACGAG 
GTGAACCTCT ACATGTACCT TTACTTCGTC ATCTTTATCA TCTTCGGCTC CTTCTTCACC 
CTCAATCTCT TCATTGGCGT CATCATTGAC AACTTCAACC AACAGAAGAA GAAGATGAGT 
ATCTGGCCCT CTCCAAAGGC TCCCTGCCCC CATCCATCTA TTCTTTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn4a ( NM_133199.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_573462.2 Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), mRNA.

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CloneID	OMu09887
Clone ID Related Accession (Same CDS sequence)	XM_006531987.2 , XM_006531988.2 , NM_133199.2 , XM_006531987.3
Accession Version	NM_133199.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	sodium channel protein type 4 subunit alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL604045.7 and BC129805.1. On Mar 31, 2007 this sequence version replaced NM_133199.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ278787.1, BC129805.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAGCT CATCTCTGCC CACCCTGGTC CCCCCTGGTC CCCACTGCCT GCGCCCCTTC 
ACCCCAGAGT CCCTGGCAGC CATAGAACAG CGGGCAATGG AGGAGGAAGC CCGGCTGCAG 
CGGAACAAGC AAATGGAGAT TGAAGAGCCT GAGCGGAAGC CACGCAGTGA CCTGGAAGCT 
GGCAAGAACC TCCCACTCAT CTATGGGGAC CCCCCACCTG AAGTCATTGG CGTCCCCCTG 
GAGGACCTGG ACCCTTACTA CAGTGACAAG AAGACCTTCA TTGTGCTCAA CAAAGGAAAG 
GCCATCTTCC GATTCTCTGC CACCCCTGCC CTCTACATGC TGAGCCCCTT CAGCATCGTC 
CGGCGGGTGG CTATCAAGGT GCTCATCCAC GCGCTGTTCA GCATGTTCAT CATGATCACC 
ATCTTGACCA ACTGCGTGTT CATGACCATG AGCAACCCGC CTTCTTGGTC CAAGGACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTT GAGTCCCTCA TTAAGATGCT GGCCCGAGGC 
TTTTGCATTG ATGACTTCAC ATTCCTCCGA GACCCCTGGA ACTGGCTGGA CTTCAGTGTC 
ATCACAATGG CGTACGTGAC AGAGTTTGTG GACTTGGGCA ACATCTCAGC CCTGAGGACC 
TTCCGTGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACGATCGTG 
GGAGCCCTGA TCCAGTCTGT GAAAAAGCTC TCGGATGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTTTTCATGG GAAACCTGCG TCAGAAGTGC 
GTGCGTTGGC CCCCGCCCAT GAATGACACC AACACCACGT GGTATGGTAA TGATACGTGG 
TACGGCAATG ACACCTGGTA CGGCAATGAC ACCTGGTATG GCAATGACAC TTGGAACAGC 
CAGGAGAGCT GGGTCAGCAA CTCTACCTTT GACTGGGAGG CCTACATCAA TGACGAAGGG 
AACTTCTATT TCTTGGAGGG CTCCAATGAT GCCCTGCTCT GTGGGAATAG CAGTGATGCC 
GGGCACTGCC CTGAGGGCTA CGAATGCATG AAGGCTGGGA GGAACCCCAA CTATGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACGCAGGAC 
TACTGGGAGA ACCTTTTCCA GCTGACCCTT CGAGCCGCTG GCAAGACCTA CATGATCTTC 
TTTGTGGTCA TCATCTTCCT GGGTTCCTTC TACCTCATCA ATCTGATCCT GGCGGTGGTG 
GCCATGGCAT ATGCTGAGCA GAATGAGGCT ACCCTGGCCG AAGACCAGGA GAAAGAGGAG 
GAGTTCCAAC AGATGCTTGA AAAATTCAAA AAACATCAGG AGGAACTGGA AAAGGCGAAG 
GCTGCCCAGG CTCTGGAAGG TGGAGAGGAG GCAGATGGGG ACCCAACCCA CAGCAAAGAC 
TGCAATGGTA GCCTGGATAC ATCCGGGGAG AAGGGGCCCC CAAGGCCAAG CTGCAGCGCA 
GAGAGTGCCA TCTCAGATGC TATGGAGGAG CTGGAAGAGG CCCATCAGAA GTGCCCCCCA 
TGGTGGTACA AGTGTGCACA CAAAGTGCTC ATCTGGAACT GCTGTGCCCC GTGGGTGAAG 
TTCAAGCATA TTATCCTCCT GATTGTCATG GACCCCTTTG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCACT ACCCCATGAC TGAGCACTTT 
GACAACGTGC TCTCTGTGGG CAACTTGGTC TTCACGGGCA TCTTCACTGC AGAGATGGTG 
CTGAAGTTGA TTGCTATGGA CCCCTACGAA TATTTCCAAC AGGGCTGGAA CATCTTCGAC 
AGCTTCATCG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAACGTGCA GGGCCTATCA 
GTGCTCCGTT CTTTCCGTCT GCTGCGTGTC TTCAAGCTGG CCAAGTCATG GCCAACACTC 
AACATGCTCA TCAAAATCAT TGGCAACTCA GTGGGTGCGC TGGGCAACCT GACCCTGGTG 
TTGGCCATCA TCGTCTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAT 
AAGGAATGCG TGTGCAAGAT TGCCTCAGAC TGCAGCCTGC CCCGCTGGCA CATGCACGAT 
TTCTTCCACT CCTTCCTCAT CGTCTTCCGC ATCCTCTGTG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAAGTGGC CGGCCAGGCC ATGTGCCTCA CCGTCTTCCT CATGGTCATG 
GTCATTGGCA ACCTGGTGGT CCTGAATCTG TTCCTGGCTC TCCTGTTGAG TTCCTTCAGC 
GCTGACAGTC TGGCAGCGTC AGATGAAGAT GGTGAGATGA ACAACCTACA GATCGCCATC 
GGGCGTATCA AGTGGGGCAT CGCCTTTGCC AAAACCTTCC TCTTGGGGCT GTTGCATGGC 
AAGATCCTGA GCCTCAAAGA CATAATGCTC AGTCTCGGGG AGCCTGGGGG TGCTGGGGAA 
AACGGGGAGA GCCCTCCCGA GGATGAGAAG AAGGAACCAC CTCCGGAAGA TGGGAACAAG 
GAGCTGAAAG ACAATCATAT CCTGAACCAC GTGGGCCTGA CTGATGGACC CCGCTCCAGC 
ATCGAGATGG ACCACCTTAA CTTCATCAAC AACCCCTACC TCACTATCCA TGTGCCCATT 
GCCTCCGAGG AGTCCGACTT GGAAATGCCC ACAGAGGAGG AGACTGACAC CTTCTCGGAG 
CCTGAGGACA TCAAGAAGCC ACTACAGCCT CTGTACGACG GGAACTCCTC CGTCTGCAGC 
ACAGCTGACT ACAAGCCCCC CGAAGAGGAC CCTGAGGAGC AGGCTGAGGA GAACCCTGAG 
GGGGAGCTAC CTGAGGAGTG TTTCACAGAG GCCTGTGTGA AGCGCTGCCC CTGCCTCTAT 
GTGGACATCT CCCAGGGCCG CGGGAAAATG TGGTGGACAC TCCGCAGGGC CTGCTTCAAG 
ATTGTTGAGC ACAACTGGTT TGAGACCTTC ATCGTCTTCA TGATCTTGCT CAGCAGCGGG 
GCCCTGGCCT TCGAGGACAT CTACATTGAA CAGCGGCGAG TCATCCAAAC CATCCTGGAA 
TATGCTGACA AGGTCTTCAC CTACATCTTC ATCCTGGAGA TGCTGCTAAA GTGGGTGGCC 
TACGGCTTCA AGGTGTACTT CACCAACGCC TGGTGCTGGC TCGACTTCCT CATTGTGGAT 
GTCTCCATCA TCAGTCTGGT GGCTAACTGG CTGGGCTACT CTGAGCTGGG GCCCATCAAA 
TCCCTGCGCA CGCTCCGGGC CCTGCGACCT CTGAGGGCAC TGTCCCGATT TGAAGGCATG 
AGGGTGGTGG TGAACGCCCT TCTGGGAGCC ATCCCTTCCA TCATGAACGT GCTCCTTGTC 
TGCCTCATCT TCTGGCTCAT CTTCAGCATC ATGGGGGTCA ACTTGTTTGC TGGAAAGTTC 
TACTACTGCA TCAACACAAC CACCTCCGAG AGATTCGACA TCTCTGTGGT CAACAACAAG 
TCCGAGTGTG AGAGCCTCAT GTACACCGGC CAGGTCCGCT GGATGAACGT CAAGGTCAAC 
TATGACAACG TGGGTCTCGG CTACCTCTCT CTTCTTCAGG TGGCCACTTT CAAGGGTTGG 
ATGGACATCA TGTATGCAGC TGTGGACTCC CGGGAGAAAG AGGAGCAGCC AGACTACGAG 
GTGAACCTCT ACATGTACCT TTACTTCGTC ATCTTTATCA TCTTCGGCTC CTTCTTCACC 
CTCAATCTCT TCATTGGCGT CATCATTGAC AACTTCAACC AACAGAAGAA GAAGTTTGGA 
GGGAAAGACA TCTTCATGAC AGAGGAACAG AAGAAATACT ACAATGCGAT GAAGAAGCTT 
GGCTCCAAGA AGCCCCAGAA GCCAATCCCC CGGCCTCAGA ACAAGATCCA AGGCATGGTG 
TACGACTTCG TGACAAAGCA GGTGTTTGAC ATCTCTATCA TGATCCTCAT CTGCCTCAAC 
ATGGTGACGA TGATGGTGGA GACGGATGAC CAGAGCCAGC TCAAGGTGGA CATCCTGTAT 
AACATCAACA TGGTCTTCAT TATCGTCTTC ACAGGGGAGT GTGTGCTCAA GATGTTTGCC 
CTGCGCCATT ACTACTTCAC CATTGGCTGG AATATCTTCG ACTTCGTGGT TGTCATCTTG 
TCCATTGTGG GTCTCGCACT CTCTGACTTG ATACAGAAAT ACTTTGTGTC ACCCACACTG 
TTCCGTGTGA TCCGATTGGC TCGGATCGGG CGTGTCTTGC GTCTGATCCG TGGGGCCAAG 
GGCATCCGGA CGCTGCTGTT CGCCCTGATG ATGTCCCTGC CCGCCCTCTT CAACATCGGC 
CTCCTTCTCT TTCTGGTCAT GTTCATTTAC TCCATCTTCG GCATGTCTAA CTTCGCCTAC 
GTCAAGAAAG AGTCGGGCAT CGACGACATG TTCAACTTTG AGACCTTTGG CAACAGTATC 
ATCTGCCTCT TCGAGATCAC CACGTCAGCT GGCTGGGACG GGCTTCTGAA CCCCATCCTC 
AACAGTGGGC CCCCAGACTG TGACCCGACA TTGGAGAACC CAGGCACCAA TATCAAGGGT 
GACTGCGGCA ACCCATCCAT CGGCATCTGT TTCTTCTGCA GCTACATCAT CATCTCCTTC 
CTCATCGTGG TCAACATGTA CATTGCCATC ATCCTAGAGA ATTTCAATGT GGCCACTGAA 
GAGAGCAGTG AGCCCCTCTG TGAGGATGAC TTCGAGATGT TCTATGAGAC CTGGGAGAAG 
TTTGACCCCG ATGCAACTCA GTTCATCGAC TACAGCCGCC TTTCTGACTT TGTGGACACC 
CTGCAGGAGC CCTTGAAAAT TGCCAAGCCC AATAAGATCA AGCTTATCAC ATTAGACCTG 
CCCATGGTAC CCGGGGACAA GATCCACTGC CTGGACATCC TCTTTGCCCT GACCAAAGAG 
GTACTGGGTG ACTCTGGGGA AATGGATGCT CTCAAACAGA CCATGGAGGA GAAGTTCATG 
GCAGCTAACC CCTCCAAGGT CTCCTATGAG CCCATCACCA CCACCCTCAA GAGGAAGCAG 
GAGGAGGTGT GTGCTATCAA AATCCAGAGG GCTTACCGCC GCCATCTGCT GCAGCGCTCT 
GTGAAGCAGG CCTCTTACAT GTACCGCCAC AGCCAGGAAG GCAATGGCGA TGGGGCCCCT 
GAGAAGGAGG GATTGCTTGC CAACACCATG AACAAGATGT ATGGCTCTGA GAAAGAGGAC 
AACGGTGTGC AGAGCCAGGG GGAGAAAGAG AAAGACTCAA CAGAGGATGC TGGACCCACC 
ACGGAGGTCA CAGCCCCCAG CAGCTCGGAC ACTGCCCTGA CTCCTCCTCC TCCTTCTCCT 
CCTCCACCAT CATCACCACC ACAAGGGCAG ACAGTGCGCC CAGGGGTCAA AGAGTCTCTT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573462.2
CDS	2..5527
Translation	MASSSLPTLVPPGPHCLRPFTPESLAAIEQRAMEEEARLQRNKQMEIEEPERKPRSDLEAGKNLPLIYGDPPPEVIGVPLEDLDPYYSDKKTFIVLNKGKAIFRFSATPALYMLSPFSIVRRVAIKVLIHALFSMFIMITILTNCVFMTMSNPPSWSKDVEYTFTGIYTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVITMAYVTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPMNDTNTTWYGNDTWYGNDTWYGNDTWYGNDTWNSQESWVSNSTFDWEAYINDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECMKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKHQEELEKAKAAQALEGGEEADGDPTHSKDCNGSLDTSGEKGPPRPSCSAESAISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWVKFKHIILLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLSVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSFIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCSLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIGRIKWGIAFAKTFLLGLLHGKILSLKDIMLSLGEPGGAGENGESPPEDEKKEPPPEDGNKELKDNHILNHVGLTDGPRSSIEMDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDIKKPLQPLYDGNSSVCSTADYKPPEEDPEEQAEENPEGELPEECFTEACVKRCPCLYVDISQGRGKMWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRRVIQTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDISVVNNKSECESLMYTGQVRWMNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPDYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKIQGMVYDFVTKQVFDISIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIVFTGECVLKMFALRHYYFTIGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPTLENPGTNIKGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLCEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLKIAKPNKIKLITLDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKQTMEEKFMAANPSKVSYEPITTTLKRKQEEVCAIKIQRAYRRHLLQRSVKQASYMYRHSQEGNGDGAPEKEGLLANTMNKMYGSEKEDNGVQSQGEKEKDSTEDAGPTTEVTAPSSSDTALTPPPPSPPPPSSPPQGQTVRPGVKESLV

Target ORF information:

RefSeq Version	NM_133199.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133199.2

ORF Insert Sequence:

ATGGCCAGCT CATCTCTGCC CACCCTGGTC CCCCCTGGTC CCCACTGCCT GCGCCCCTTC 
ACCCCAGAGT CCCTGGCAGC CATAGAACAG CGGGCAATGG AGGAGGAAGC CCGGCTGCAG 
CGGAACAAGC AAATGGAGAT TGAAGAGCCT GAGCGGAAGC CACGCAGTGA CCTGGAAGCT 
GGCAAGAACC TCCCACTCAT CTATGGGGAC CCCCCACCTG AAGTCATTGG CGTCCCCCTG 
GAGGACCTGG ACCCTTACTA CAGTGACAAG AAGACCTTCA TTGTGCTCAA CAAAGGAAAG 
GCCATCTTCC GATTCTCTGC CACCCCTGCC CTCTACATGC TGAGCCCCTT CAGCATCGTC 
CGGCGGGTGG CTATCAAGGT GCTCATCCAC GCGCTGTTCA GCATGTTCAT CATGATCACC 
ATCTTGACCA ACTGCGTGTT CATGACCATG AGCAACCCGC CTTCTTGGTC CAAGGACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTT GAGTCCCTCA TTAAGATGCT GGCCCGAGGC 
TTTTGCATTG ATGACTTCAC ATTCCTCCGA GACCCCTGGA ACTGGCTGGA CTTCAGTGTC 
ATCACAATGG CGTACGTGAC AGAGTTTGTG GACTTGGGCA ACATCTCAGC CCTGAGGACC 
TTCCGTGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACGATCGTG 
GGAGCCCTGA TCCAGTCTGT GAAAAAGCTC TCGGATGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTTTTCATGG GAAACCTGCG TCAGAAGTGC 
GTGCGTTGGC CCCCGCCCAT GAATGACACC AACACCACGT GGTATGGTAA TGATACGTGG 
TACGGCAATG ACACCTGGTA CGGCAATGAC ACCTGGTATG GCAATGACAC TTGGAACAGC 
CAGGAGAGCT GGGTCAGCAA CTCTACCTTT GACTGGGAGG CCTACATCAA TGACGAAGGG 
AACTTCTATT TCTTGGAGGG CTCCAATGAT GCCCTGCTCT GTGGGAATAG CAGTGATGCC 
GGGCACTGCC CTGAGGGCTA CGAATGCATG AAGGCTGGGA GGAACCCCAA CTATGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACGCAGGAC 
TACTGGGAGA ACCTTTTCCA GCTGACCCTT CGAGCCGCTG GCAAGACCTA CATGATCTTC 
TTTGTGGTCA TCATCTTCCT GGGTTCCTTC TACCTCATCA ATCTGATCCT GGCGGTGGTG 
GCCATGGCAT ATGCTGAGCA GAATGAGGCT ACCCTGGCCG AAGACCAGGA GAAAGAGGAG 
GAGTTCCAAC AGATGCTTGA AAAATTCAAA AAACATCAGG AGGAACTGGA AAAGGCGAAG 
GCTGCCCAGG CTCTGGAAGG TGGAGAGGAG GCAGATGGGG ACCCAACCCA CAGCAAAGAC 
TGCAATGGTA GCCTGGATAC ATCCGGGGAG AAGGGGCCCC CAAGGCCAAG CTGCAGCGCA 
GAGAGTGCCA TCTCAGATGC TATGGAGGAG CTGGAAGAGG CCCATCAGAA GTGCCCCCCA 
TGGTGGTACA AGTGTGCACA CAAAGTGCTC ATCTGGAACT GCTGTGCCCC GTGGGTGAAG 
TTCAAGCATA TTATCCTCCT GATTGTCATG GACCCCTTTG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCACT ACCCCATGAC TGAGCACTTT 
GACAACGTGC TCTCTGTGGG CAACTTGGTC TTCACGGGCA TCTTCACTGC AGAGATGGTG 
CTGAAGTTGA TTGCTATGGA CCCCTACGAA TATTTCCAAC AGGGCTGGAA CATCTTCGAC 
AGCTTCATCG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAACGTGCA GGGCCTATCA 
GTGCTCCGTT CTTTCCGTCT GCTGCGTGTC TTCAAGCTGG CCAAGTCATG GCCAACACTC 
AACATGCTCA TCAAAATCAT TGGCAACTCA GTGGGTGCGC TGGGCAACCT GACCCTGGTG 
TTGGCCATCA TCGTCTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAT 
AAGGAATGCG TGTGCAAGAT TGCCTCAGAC TGCAGCCTGC CCCGCTGGCA CATGCACGAT 
TTCTTCCACT CCTTCCTCAT CGTCTTCCGC ATCCTCTGTG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAAGTGGC CGGCCAGGCC ATGTGCCTCA CCGTCTTCCT CATGGTCATG 
GTCATTGGCA ACCTGGTGGT CCTGAATCTG TTCCTGGCTC TCCTGTTGAG TTCCTTCAGC 
GCTGACAGTC TGGCAGCGTC AGATGAAGAT GGTGAGATGA ACAACCTACA GATCGCCATC 
GGGCGTATCA AGTGGGGCAT CGCCTTTGCC AAAACCTTCC TCTTGGGGCT GTTGCATGGC 
AAGATCCTGA GCCTCAAAGA CATAATGCTC AGTCTCGGGG AGCCTGGGGG TGCTGGGGAA 
AACGGGGAGA GCCCTCCCGA GGATGAGAAG AAGGAACCAC CTCCGGAAGA TGGGAACAAG 
GAGCTGAAAG ACAATCATAT CCTGAACCAC GTGGGCCTGA CTGATGGACC CCGCTCCAGC 
ATCGAGATGG ACCACCTTAA CTTCATCAAC AACCCCTACC TCACTATCCA TGTGCCCATT 
GCCTCCGAGG AGTCCGACTT GGAAATGCCC ACAGAGGAGG AGACTGACAC CTTCTCGGAG 
CCTGAGGACA TCAAGAAGCC ACTACAGCCT CTGTACGACG GGAACTCCTC CGTCTGCAGC 
ACAGCTGACT ACAAGCCCCC CGAAGAGGAC CCTGAGGAGC AGGCTGAGGA GAACCCTGAG 
GGGGAGCTAC CTGAGGAGTG TTTCACAGAG GCCTGTGTGA AGCGCTGCCC CTGCCTCTAT 
GTGGACATCT CCCAGGGCCG CGGGAAAATG TGGTGGACAC TCCGCAGGGC CTGCTTCAAG 
ATTGTTGAGC ACAACTGGTT TGAGACCTTC ATCGTCTTCA TGATCTTGCT CAGCAGCGGG 
GCCCTGGCCT TCGAGGACAT CTACATTGAA CAGCGGCGAG TCATCCAAAC CATCCTGGAA 
TATGCTGACA AGGTCTTCAC CTACATCTTC ATCCTGGAGA TGCTGCTAAA GTGGGTGGCC 
TACGGCTTCA AGGTGTACTT CACCAACGCC TGGTGCTGGC TCGACTTCCT CATTGTGGAT 
GTCTCCATCA TCAGTCTGGT GGCTAACTGG CTGGGCTACT CTGAGCTGGG GCCCATCAAA 
TCCCTGCGCA CGCTCCGGGC CCTGCGACCT CTGAGGGCAC TGTCCCGATT TGAAGGCATG 
AGGGTGGTGG TGAACGCCCT TCTGGGAGCC ATCCCTTCCA TCATGAACGT GCTCCTTGTC 
TGCCTCATCT TCTGGCTCAT CTTCAGCATC ATGGGGGTCA ACTTGTTTGC TGGAAAGTTC 
TACTACTGCA TCAACACAAC CACCTCCGAG AGATTCGACA TCTCTGTGGT CAACAACAAG 
TCCGAGTGTG AGAGCCTCAT GTACACCGGC CAGGTCCGCT GGATGAACGT CAAGGTCAAC 
TATGACAACG TGGGTCTCGG CTACCTCTCT CTTCTTCAGG TGGCCACTTT CAAGGGTTGG 
ATGGACATCA TGTATGCAGC TGTGGACTCC CGGGAGAAAG AGGAGCAGCC AGACTACGAG 
GTGAACCTCT ACATGTACCT TTACTTCGTC ATCTTTATCA TCTTCGGCTC CTTCTTCACC 
CTCAATCTCT TCATTGGCGT CATCATTGAC AACTTCAACC AACAGAAGAA GAAGTTTGGA 
GGGAAAGACA TCTTCATGAC AGAGGAACAG AAGAAATACT ACAATGCGAT GAAGAAGCTT 
GGCTCCAAGA AGCCCCAGAA GCCAATCCCC CGGCCTCAGA ACAAGATCCA AGGCATGGTG 
TACGACTTCG TGACAAAGCA GGTGTTTGAC ATCTCTATCA TGATCCTCAT CTGCCTCAAC 
ATGGTGACGA TGATGGTGGA GACGGATGAC CAGAGCCAGC TCAAGGTGGA CATCCTGTAT 
AACATCAACA TGGTCTTCAT TATCGTCTTC ACAGGGGAGT GTGTGCTCAA GATGTTTGCC 
CTGCGCCATT ACTACTTCAC CATTGGCTGG AATATCTTCG ACTTCGTGGT TGTCATCTTG 
TCCATTGTGG GTCTCGCACT CTCTGACTTG ATACAGAAAT ACTTTGTGTC ACCCACACTG 
TTCCGTGTGA TCCGATTGGC TCGGATCGGG CGTGTCTTGC GTCTGATCCG TGGGGCCAAG 
GGCATCCGGA CGCTGCTGTT CGCCCTGATG ATGTCCCTGC CCGCCCTCTT CAACATCGGC 
CTCCTTCTCT TTCTGGTCAT GTTCATTTAC TCCATCTTCG GCATGTCTAA CTTCGCCTAC 
GTCAAGAAAG AGTCGGGCAT CGACGACATG TTCAACTTTG AGACCTTTGG CAACAGTATC 
ATCTGCCTCT TCGAGATCAC CACGTCAGCT GGCTGGGACG GGCTTCTGAA CCCCATCCTC 
AACAGTGGGC CCCCAGACTG TGACCCGACA TTGGAGAACC CAGGCACCAA TATCAAGGGT 
GACTGCGGCA ACCCATCCAT CGGCATCTGT TTCTTCTGCA GCTACATCAT CATCTCCTTC 
CTCATCGTGG TCAACATGTA CATTGCCATC ATCCTAGAGA ATTTCAATGT GGCCACTGAA 
GAGAGCAGTG AGCCCCTCTG TGAGGATGAC TTCGAGATGT TCTATGAGAC CTGGGAGAAG 
TTTGACCCCG ATGCAACTCA GTTCATCGAC TACAGCCGCC TTTCTGACTT TGTGGACACC 
CTGCAGGAGC CCTTGAAAAT TGCCAAGCCC AATAAGATCA AGCTTATCAC ATTAGACCTG 
CCCATGGTAC CCGGGGACAA GATCCACTGC CTGGACATCC TCTTTGCCCT GACCAAAGAG 
GTACTGGGTG ACTCTGGGGA AATGGATGCT CTCAAACAGA CCATGGAGGA GAAGTTCATG 
GCAGCTAACC CCTCCAAGGT CTCCTATGAG CCCATCACCA CCACCCTCAA GAGGAAGCAG 
GAGGAGGTGT GTGCTATCAA AATCCAGAGG GCTTACCGCC GCCATCTGCT GCAGCGCTCT 
GTGAAGCAGG CCTCTTACAT GTACCGCCAC AGCCAGGAAG GCAATGGCGA TGGGGCCCCT 
GAGAAGGAGG GATTGCTTGC CAACACCATG AACAAGATGT ATGGCTCTGA GAAAGAGGAC 
AACGGTGTGC AGAGCCAGGG GGAGAAAGAG AAAGACTCAA CAGAGGATGC TGGACCCACC 
ACGGAGGTCA CAGCCCCCAG CAGCTCGGAC ACTGCCCTGA CTCCTCCTCC TCCTTCTCCT 
CCTCCACCAT CATCACCACC ACAAGGGCAG ACAGTGCGCC CAGGGGTCAA AGAGTCTCTT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn4a ( XM_006531987.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532050.1 Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X2, mRNA.

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CloneID	OMu09887
Clone ID Related Accession (Same CDS sequence)	XM_006531987.2 , XM_006531988.2 , NM_133199.2 , XM_006531987.3
Accession Version	XM_006531987.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sodium channel protein type 4 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006531987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CATCTCTGCC CACCCTGGTC CCCCCTGGTC CCCACTGCCT GCGCCCCTTC 
ACCCCAGAGT CCCTGGCAGC CATAGAACAG CGGGCAATGG AGGAGGAAGC CCGGCTGCAG 
CGGAACAAGC AAATGGAGAT TGAAGAGCCT GAGCGGAAGC CACGCAGTGA CCTGGAAGCT 
GGCAAGAACC TCCCACTCAT CTATGGGGAC CCCCCACCTG AAGTCATTGG CGTCCCCCTG 
GAGGACCTGG ACCCTTACTA CAGTGACAAG AAGACCTTCA TTGTGCTCAA CAAAGGAAAG 
GCCATCTTCC GATTCTCTGC CACCCCTGCC CTCTACATGC TGAGCCCCTT CAGCATCGTC 
CGGCGGGTGG CTATCAAGGT GCTCATCCAC GCGCTGTTCA GCATGTTCAT CATGATCACC 
ATCTTGACCA ACTGCGTGTT CATGACCATG AGCAACCCGC CTTCTTGGTC CAAGGACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTT GAGTCCCTCA TTAAGATGCT GGCCCGAGGC 
TTTTGCATTG ATGACTTCAC ATTCCTCCGA GACCCCTGGA ACTGGCTGGA CTTCAGTGTC 
ATCACAATGG CGTACGTGAC AGAGTTTGTG GACTTGGGCA ACATCTCAGC CCTGAGGACC 
TTCCGTGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACGATCGTG 
GGAGCCCTGA TCCAGTCTGT GAAAAAGCTC TCGGATGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTTTTCATGG GAAACCTGCG TCAGAAGTGC 
GTGCGTTGGC CCCCGCCCAT GAATGACACC AACACCACGT GGTATGGTAA TGATACGTGG 
TACGGCAATG ACACCTGGTA CGGCAATGAC ACCTGGTATG GCAATGACAC TTGGAACAGC 
CAGGAGAGCT GGGTCAGCAA CTCTACCTTT GACTGGGAGG CCTACATCAA TGACGAAGGG 
AACTTCTATT TCTTGGAGGG CTCCAATGAT GCCCTGCTCT GTGGGAATAG CAGTGATGCC 
GGGCACTGCC CTGAGGGCTA CGAATGCATG AAGGCTGGGA GGAACCCCAA CTATGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACGCAGGAC 
TACTGGGAGA ACCTTTTCCA GCTGACCCTT CGAGCCGCTG GCAAGACCTA CATGATCTTC 
TTTGTGGTCA TCATCTTCCT GGGTTCCTTC TACCTCATCA ATCTGATCCT GGCGGTGGTG 
GCCATGGCAT ATGCTGAGCA GAATGAGGCT ACCCTGGCCG AAGACCAGGA GAAAGAGGAG 
GAGTTCCAAC AGATGCTTGA AAAATTCAAA AAACATCAGG AGGAACTGGA AAAGGCGAAG 
GCTGCCCAGG CTCTGGAAGG TGGAGAGGAG GCAGATGGGG ACCCAACCCA CAGCAAAGAC 
TGCAATGGTA GCCTGGATAC ATCCGGGGAG AAGGGGCCCC CAAGGCCAAG CTGCAGCGCA 
GAGAGTGCCA TCTCAGATGC TATGGAGGAG CTGGAAGAGG CCCATCAGAA GTGCCCCCCA 
TGGTGGTACA AGTGTGCACA CAAAGTGCTC ATCTGGAACT GCTGTGCCCC GTGGGTGAAG 
TTCAAGCATA TTATCCTCCT GATTGTCATG GACCCCTTTG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCACT ACCCCATGAC TGAGCACTTT 
GACAACGTGC TCTCTGTGGG CAACTTGGTC TTCACGGGCA TCTTCACTGC AGAGATGGTG 
CTGAAGTTGA TTGCTATGGA CCCCTACGAA TATTTCCAAC AGGGCTGGAA CATCTTCGAC 
AGCTTCATCG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAACGTGCA GGGCCTATCA 
GTGCTCCGTT CTTTCCGTCT GCTGCGTGTC TTCAAGCTGG CCAAGTCATG GCCAACACTC 
AACATGCTCA TCAAAATCAT TGGCAACTCA GTGGGTGCGC TGGGCAACCT GACCCTGGTG 
TTGGCCATCA TCGTCTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAT 
AAGGAATGCG TGTGCAAGAT TGCCTCAGAC TGCAGCCTGC CCCGCTGGCA CATGCACGAT 
TTCTTCCACT CCTTCCTCAT CGTCTTCCGC ATCCTCTGTG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAAGTGGC CGGCCAGGCC ATGTGCCTCA CCGTCTTCCT CATGGTCATG 
GTCATTGGCA ACCTGGTGGT CCTGAATCTG TTCCTGGCTC TCCTGTTGAG TTCCTTCAGC 
GCTGACAGTC TGGCAGCGTC AGATGAAGAT GGTGAGATGA ACAACCTACA GATCGCCATC 
GGGCGTATCA AGTGGGGCAT CGCCTTTGCC AAAACCTTCC TCTTGGGGCT GTTGCATGGC 
AAGATCCTGA GCCTCAAAGA CATAATGCTC AGTCTCGGGG AGCCTGGGGG TGCTGGGGAA 
AACGGGGAGA GCCCTCCCGA GGATGAGAAG AAGGAACCAC CTCCGGAAGA TGGGAACAAG 
GAGCTGAAAG ACAATCATAT CCTGAACCAC GTGGGCCTGA CTGATGGACC CCGCTCCAGC 
ATCGAGATGG ACCACCTTAA CTTCATCAAC AACCCCTACC TCACTATCCA TGTGCCCATT 
GCCTCCGAGG AGTCCGACTT GGAAATGCCC ACAGAGGAGG AGACTGACAC CTTCTCGGAG 
CCTGAGGACA TCAAGAAGCC ACTACAGCCT CTGTACGACG GGAACTCCTC CGTCTGCAGC 
ACAGCTGACT ACAAGCCCCC CGAAGAGGAC CCTGAGGAGC AGGCTGAGGA GAACCCTGAG 
GGGGAGCTAC CTGAGGAGTG TTTCACAGAG GCCTGTGTGA AGCGCTGCCC CTGCCTCTAT 
GTGGACATCT CCCAGGGCCG CGGGAAAATG TGGTGGACAC TCCGCAGGGC CTGCTTCAAG 
ATTGTTGAGC ACAACTGGTT TGAGACCTTC ATCGTCTTCA TGATCTTGCT CAGCAGCGGG 
GCCCTGGCCT TCGAGGACAT CTACATTGAA CAGCGGCGAG TCATCCAAAC CATCCTGGAA 
TATGCTGACA AGGTCTTCAC CTACATCTTC ATCCTGGAGA TGCTGCTAAA GTGGGTGGCC 
TACGGCTTCA AGGTGTACTT CACCAACGCC TGGTGCTGGC TCGACTTCCT CATTGTGGAT 
GTCTCCATCA TCAGTCTGGT GGCTAACTGG CTGGGCTACT CTGAGCTGGG GCCCATCAAA 
TCCCTGCGCA CGCTCCGGGC CCTGCGACCT CTGAGGGCAC TGTCCCGATT TGAAGGCATG 
AGGGTGGTGG TGAACGCCCT TCTGGGAGCC ATCCCTTCCA TCATGAACGT GCTCCTTGTC 
TGCCTCATCT TCTGGCTCAT CTTCAGCATC ATGGGGGTCA ACTTGTTTGC TGGAAAGTTC 
TACTACTGCA TCAACACAAC CACCTCCGAG AGATTCGACA TCTCTGTGGT CAACAACAAG 
TCCGAGTGTG AGAGCCTCAT GTACACCGGC CAGGTCCGCT GGATGAACGT CAAGGTCAAC 
TATGACAACG TGGGTCTCGG CTACCTCTCT CTTCTTCAGG TGGCCACTTT CAAGGGTTGG 
ATGGACATCA TGTATGCAGC TGTGGACTCC CGGGAGAAAG AGGAGCAGCC AGACTACGAG 
GTGAACCTCT ACATGTACCT TTACTTCGTC ATCTTTATCA TCTTCGGCTC CTTCTTCACC 
CTCAATCTCT TCATTGGCGT CATCATTGAC AACTTCAACC AACAGAAGAA GAAGTTTGGA 
GGGAAAGACA TCTTCATGAC AGAGGAACAG AAGAAATACT ACAATGCGAT GAAGAAGCTT 
GGCTCCAAGA AGCCCCAGAA GCCAATCCCC CGGCCTCAGA ACAAGATCCA AGGCATGGTG 
TACGACTTCG TGACAAAGCA GGTGTTTGAC ATCTCTATCA TGATCCTCAT CTGCCTCAAC 
ATGGTGACGA TGATGGTGGA GACGGATGAC CAGAGCCAGC TCAAGGTGGA CATCCTGTAT 
AACATCAACA TGGTCTTCAT TATCGTCTTC ACAGGGGAGT GTGTGCTCAA GATGTTTGCC 
CTGCGCCATT ACTACTTCAC CATTGGCTGG AATATCTTCG ACTTCGTGGT TGTCATCTTG 
TCCATTGTGG GTCTCGCACT CTCTGACTTG ATACAGAAAT ACTTTGTGTC ACCCACACTG 
TTCCGTGTGA TCCGATTGGC TCGGATCGGG CGTGTCTTGC GTCTGATCCG TGGGGCCAAG 
GGCATCCGGA CGCTGCTGTT CGCCCTGATG ATGTCCCTGC CCGCCCTCTT CAACATCGGC 
CTCCTTCTCT TTCTGGTCAT GTTCATTTAC TCCATCTTCG GCATGTCTAA CTTCGCCTAC 
GTCAAGAAAG AGTCGGGCAT CGACGACATG TTCAACTTTG AGACCTTTGG CAACAGTATC 
ATCTGCCTCT TCGAGATCAC CACGTCAGCT GGCTGGGACG GGCTTCTGAA CCCCATCCTC 
AACAGTGGGC CCCCAGACTG TGACCCGACA TTGGAGAACC CAGGCACCAA TATCAAGGGT 
GACTGCGGCA ACCCATCCAT CGGCATCTGT TTCTTCTGCA GCTACATCAT CATCTCCTTC 
CTCATCGTGG TCAACATGTA CATTGCCATC ATCCTAGAGA ATTTCAATGT GGCCACTGAA 
GAGAGCAGTG AGCCCCTCTG TGAGGATGAC TTCGAGATGT TCTATGAGAC CTGGGAGAAG 
TTTGACCCCG ATGCAACTCA GTTCATCGAC TACAGCCGCC TTTCTGACTT TGTGGACACC 
CTGCAGGAGC CCTTGAAAAT TGCCAAGCCC AATAAGATCA AGCTTATCAC ATTAGACCTG 
CCCATGGTAC CCGGGGACAA GATCCACTGC CTGGACATCC TCTTTGCCCT GACCAAAGAG 
GTACTGGGTG ACTCTGGGGA AATGGATGCT CTCAAACAGA CCATGGAGGA GAAGTTCATG 
GCAGCTAACC CCTCCAAGGT CTCCTATGAG CCCATCACCA CCACCCTCAA GAGGAAGCAG 
GAGGAGGTGT GTGCTATCAA AATCCAGAGG GCTTACCGCC GCCATCTGCT GCAGCGCTCT 
GTGAAGCAGG CCTCTTACAT GTACCGCCAC AGCCAGGAAG GCAATGGCGA TGGGGCCCCT 
GAGAAGGAGG GATTGCTTGC CAACACCATG AACAAGATGT ATGGCTCTGA GAAAGAGGAC 
AACGGTGTGC AGAGCCAGGG GGAGAAAGAG AAAGACTCAA CAGAGGATGC TGGACCCACC 
ACGGAGGTCA CAGCCCCCAG CAGCTCGGAC ACTGCCCTGA CTCCTCCTCC TCCTTCTCCT 
CCTCCACCAT CATCACCACC ACAAGGGCAG ACAGTGCGCC CAGGGGTCAA AGAGTCTCTT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532050.1
CDS	481..6006
Translation	MASSSLPTLVPPGPHCLRPFTPESLAAIEQRAMEEEARLQRNKQMEIEEPERKPRSDLEAGKNLPLIYGDPPPEVIGVPLEDLDPYYSDKKTFIVLNKGKAIFRFSATPALYMLSPFSIVRRVAIKVLIHALFSMFIMITILTNCVFMTMSNPPSWSKDVEYTFTGIYTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVITMAYVTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPMNDTNTTWYGNDTWYGNDTWYGNDTWYGNDTWNSQESWVSNSTFDWEAYINDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECMKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKHQEELEKAKAAQALEGGEEADGDPTHSKDCNGSLDTSGEKGPPRPSCSAESAISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWVKFKHIILLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLSVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSFIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCSLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIGRIKWGIAFAKTFLLGLLHGKILSLKDIMLSLGEPGGAGENGESPPEDEKKEPPPEDGNKELKDNHILNHVGLTDGPRSSIEMDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDIKKPLQPLYDGNSSVCSTADYKPPEEDPEEQAEENPEGELPEECFTEACVKRCPCLYVDISQGRGKMWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRRVIQTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDISVVNNKSECESLMYTGQVRWMNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPDYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKIQGMVYDFVTKQVFDISIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIVFTGECVLKMFALRHYYFTIGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPTLENPGTNIKGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLCEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLKIAKPNKIKLITLDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKQTMEEKFMAANPSKVSYEPITTTLKRKQEEVCAIKIQRAYRRHLLQRSVKQASYMYRHSQEGNGDGAPEKEGLLANTMNKMYGSEKEDNGVQSQGEKEKDSTEDAGPTTEVTAPSSSDTALTPPPPSPPPPSSPPQGQTVRPGVKESLV

Target ORF information:

RefSeq Version	XM_006531987.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531987.2

ORF Insert Sequence:

ATGGCCAGCT CATCTCTGCC CACCCTGGTC CCCCCTGGTC CCCACTGCCT GCGCCCCTTC 
ACCCCAGAGT CCCTGGCAGC CATAGAACAG CGGGCAATGG AGGAGGAAGC CCGGCTGCAG 
CGGAACAAGC AAATGGAGAT TGAAGAGCCT GAGCGGAAGC CACGCAGTGA CCTGGAAGCT 
GGCAAGAACC TCCCACTCAT CTATGGGGAC CCCCCACCTG AAGTCATTGG CGTCCCCCTG 
GAGGACCTGG ACCCTTACTA CAGTGACAAG AAGACCTTCA TTGTGCTCAA CAAAGGAAAG 
GCCATCTTCC GATTCTCTGC CACCCCTGCC CTCTACATGC TGAGCCCCTT CAGCATCGTC 
CGGCGGGTGG CTATCAAGGT GCTCATCCAC GCGCTGTTCA GCATGTTCAT CATGATCACC 
ATCTTGACCA ACTGCGTGTT CATGACCATG AGCAACCCGC CTTCTTGGTC CAAGGACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTT GAGTCCCTCA TTAAGATGCT GGCCCGAGGC 
TTTTGCATTG ATGACTTCAC ATTCCTCCGA GACCCCTGGA ACTGGCTGGA CTTCAGTGTC 
ATCACAATGG CGTACGTGAC AGAGTTTGTG GACTTGGGCA ACATCTCAGC CCTGAGGACC 
TTCCGTGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACGATCGTG 
GGAGCCCTGA TCCAGTCTGT GAAAAAGCTC TCGGATGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTTTTCATGG GAAACCTGCG TCAGAAGTGC 
GTGCGTTGGC CCCCGCCCAT GAATGACACC AACACCACGT GGTATGGTAA TGATACGTGG 
TACGGCAATG ACACCTGGTA CGGCAATGAC ACCTGGTATG GCAATGACAC TTGGAACAGC 
CAGGAGAGCT GGGTCAGCAA CTCTACCTTT GACTGGGAGG CCTACATCAA TGACGAAGGG 
AACTTCTATT TCTTGGAGGG CTCCAATGAT GCCCTGCTCT GTGGGAATAG CAGTGATGCC 
GGGCACTGCC CTGAGGGCTA CGAATGCATG AAGGCTGGGA GGAACCCCAA CTATGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACGCAGGAC 
TACTGGGAGA ACCTTTTCCA GCTGACCCTT CGAGCCGCTG GCAAGACCTA CATGATCTTC 
TTTGTGGTCA TCATCTTCCT GGGTTCCTTC TACCTCATCA ATCTGATCCT GGCGGTGGTG 
GCCATGGCAT ATGCTGAGCA GAATGAGGCT ACCCTGGCCG AAGACCAGGA GAAAGAGGAG 
GAGTTCCAAC AGATGCTTGA AAAATTCAAA AAACATCAGG AGGAACTGGA AAAGGCGAAG 
GCTGCCCAGG CTCTGGAAGG TGGAGAGGAG GCAGATGGGG ACCCAACCCA CAGCAAAGAC 
TGCAATGGTA GCCTGGATAC ATCCGGGGAG AAGGGGCCCC CAAGGCCAAG CTGCAGCGCA 
GAGAGTGCCA TCTCAGATGC TATGGAGGAG CTGGAAGAGG CCCATCAGAA GTGCCCCCCA 
TGGTGGTACA AGTGTGCACA CAAAGTGCTC ATCTGGAACT GCTGTGCCCC GTGGGTGAAG 
TTCAAGCATA TTATCCTCCT GATTGTCATG GACCCCTTTG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCACT ACCCCATGAC TGAGCACTTT 
GACAACGTGC TCTCTGTGGG CAACTTGGTC TTCACGGGCA TCTTCACTGC AGAGATGGTG 
CTGAAGTTGA TTGCTATGGA CCCCTACGAA TATTTCCAAC AGGGCTGGAA CATCTTCGAC 
AGCTTCATCG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAACGTGCA GGGCCTATCA 
GTGCTCCGTT CTTTCCGTCT GCTGCGTGTC TTCAAGCTGG CCAAGTCATG GCCAACACTC 
AACATGCTCA TCAAAATCAT TGGCAACTCA GTGGGTGCGC TGGGCAACCT GACCCTGGTG 
TTGGCCATCA TCGTCTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAT 
AAGGAATGCG TGTGCAAGAT TGCCTCAGAC TGCAGCCTGC CCCGCTGGCA CATGCACGAT 
TTCTTCCACT CCTTCCTCAT CGTCTTCCGC ATCCTCTGTG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAAGTGGC CGGCCAGGCC ATGTGCCTCA CCGTCTTCCT CATGGTCATG 
GTCATTGGCA ACCTGGTGGT CCTGAATCTG TTCCTGGCTC TCCTGTTGAG TTCCTTCAGC 
GCTGACAGTC TGGCAGCGTC AGATGAAGAT GGTGAGATGA ACAACCTACA GATCGCCATC 
GGGCGTATCA AGTGGGGCAT CGCCTTTGCC AAAACCTTCC TCTTGGGGCT GTTGCATGGC 
AAGATCCTGA GCCTCAAAGA CATAATGCTC AGTCTCGGGG AGCCTGGGGG TGCTGGGGAA 
AACGGGGAGA GCCCTCCCGA GGATGAGAAG AAGGAACCAC CTCCGGAAGA TGGGAACAAG 
GAGCTGAAAG ACAATCATAT CCTGAACCAC GTGGGCCTGA CTGATGGACC CCGCTCCAGC 
ATCGAGATGG ACCACCTTAA CTTCATCAAC AACCCCTACC TCACTATCCA TGTGCCCATT 
GCCTCCGAGG AGTCCGACTT GGAAATGCCC ACAGAGGAGG AGACTGACAC CTTCTCGGAG 
CCTGAGGACA TCAAGAAGCC ACTACAGCCT CTGTACGACG GGAACTCCTC CGTCTGCAGC 
ACAGCTGACT ACAAGCCCCC CGAAGAGGAC CCTGAGGAGC AGGCTGAGGA GAACCCTGAG 
GGGGAGCTAC CTGAGGAGTG TTTCACAGAG GCCTGTGTGA AGCGCTGCCC CTGCCTCTAT 
GTGGACATCT CCCAGGGCCG CGGGAAAATG TGGTGGACAC TCCGCAGGGC CTGCTTCAAG 
ATTGTTGAGC ACAACTGGTT TGAGACCTTC ATCGTCTTCA TGATCTTGCT CAGCAGCGGG 
GCCCTGGCCT TCGAGGACAT CTACATTGAA CAGCGGCGAG TCATCCAAAC CATCCTGGAA 
TATGCTGACA AGGTCTTCAC CTACATCTTC ATCCTGGAGA TGCTGCTAAA GTGGGTGGCC 
TACGGCTTCA AGGTGTACTT CACCAACGCC TGGTGCTGGC TCGACTTCCT CATTGTGGAT 
GTCTCCATCA TCAGTCTGGT GGCTAACTGG CTGGGCTACT CTGAGCTGGG GCCCATCAAA 
TCCCTGCGCA CGCTCCGGGC CCTGCGACCT CTGAGGGCAC TGTCCCGATT TGAAGGCATG 
AGGGTGGTGG TGAACGCCCT TCTGGGAGCC ATCCCTTCCA TCATGAACGT GCTCCTTGTC 
TGCCTCATCT TCTGGCTCAT CTTCAGCATC ATGGGGGTCA ACTTGTTTGC TGGAAAGTTC 
TACTACTGCA TCAACACAAC CACCTCCGAG AGATTCGACA TCTCTGTGGT CAACAACAAG 
TCCGAGTGTG AGAGCCTCAT GTACACCGGC CAGGTCCGCT GGATGAACGT CAAGGTCAAC 
TATGACAACG TGGGTCTCGG CTACCTCTCT CTTCTTCAGG TGGCCACTTT CAAGGGTTGG 
ATGGACATCA TGTATGCAGC TGTGGACTCC CGGGAGAAAG AGGAGCAGCC AGACTACGAG 
GTGAACCTCT ACATGTACCT TTACTTCGTC ATCTTTATCA TCTTCGGCTC CTTCTTCACC 
CTCAATCTCT TCATTGGCGT CATCATTGAC AACTTCAACC AACAGAAGAA GAAGTTTGGA 
GGGAAAGACA TCTTCATGAC AGAGGAACAG AAGAAATACT ACAATGCGAT GAAGAAGCTT 
GGCTCCAAGA AGCCCCAGAA GCCAATCCCC CGGCCTCAGA ACAAGATCCA AGGCATGGTG 
TACGACTTCG TGACAAAGCA GGTGTTTGAC ATCTCTATCA TGATCCTCAT CTGCCTCAAC 
ATGGTGACGA TGATGGTGGA GACGGATGAC CAGAGCCAGC TCAAGGTGGA CATCCTGTAT 
AACATCAACA TGGTCTTCAT TATCGTCTTC ACAGGGGAGT GTGTGCTCAA GATGTTTGCC 
CTGCGCCATT ACTACTTCAC CATTGGCTGG AATATCTTCG ACTTCGTGGT TGTCATCTTG 
TCCATTGTGG GTCTCGCACT CTCTGACTTG ATACAGAAAT ACTTTGTGTC ACCCACACTG 
TTCCGTGTGA TCCGATTGGC TCGGATCGGG CGTGTCTTGC GTCTGATCCG TGGGGCCAAG 
GGCATCCGGA CGCTGCTGTT CGCCCTGATG ATGTCCCTGC CCGCCCTCTT CAACATCGGC 
CTCCTTCTCT TTCTGGTCAT GTTCATTTAC TCCATCTTCG GCATGTCTAA CTTCGCCTAC 
GTCAAGAAAG AGTCGGGCAT CGACGACATG TTCAACTTTG AGACCTTTGG CAACAGTATC 
ATCTGCCTCT TCGAGATCAC CACGTCAGCT GGCTGGGACG GGCTTCTGAA CCCCATCCTC 
AACAGTGGGC CCCCAGACTG TGACCCGACA TTGGAGAACC CAGGCACCAA TATCAAGGGT 
GACTGCGGCA ACCCATCCAT CGGCATCTGT TTCTTCTGCA GCTACATCAT CATCTCCTTC 
CTCATCGTGG TCAACATGTA CATTGCCATC ATCCTAGAGA ATTTCAATGT GGCCACTGAA 
GAGAGCAGTG AGCCCCTCTG TGAGGATGAC TTCGAGATGT TCTATGAGAC CTGGGAGAAG 
TTTGACCCCG ATGCAACTCA GTTCATCGAC TACAGCCGCC TTTCTGACTT TGTGGACACC 
CTGCAGGAGC CCTTGAAAAT TGCCAAGCCC AATAAGATCA AGCTTATCAC ATTAGACCTG 
CCCATGGTAC CCGGGGACAA GATCCACTGC CTGGACATCC TCTTTGCCCT GACCAAAGAG 
GTACTGGGTG ACTCTGGGGA AATGGATGCT CTCAAACAGA CCATGGAGGA GAAGTTCATG 
GCAGCTAACC CCTCCAAGGT CTCCTATGAG CCCATCACCA CCACCCTCAA GAGGAAGCAG 
GAGGAGGTGT GTGCTATCAA AATCCAGAGG GCTTACCGCC GCCATCTGCT GCAGCGCTCT 
GTGAAGCAGG CCTCTTACAT GTACCGCCAC AGCCAGGAAG GCAATGGCGA TGGGGCCCCT 
GAGAAGGAGG GATTGCTTGC CAACACCATG AACAAGATGT ATGGCTCTGA GAAAGAGGAC 
AACGGTGTGC AGAGCCAGGG GGAGAAAGAG AAAGACTCAA CAGAGGATGC TGGACCCACC 
ACGGAGGTCA CAGCCCCCAG CAGCTCGGAC ACTGCCCTGA CTCCTCCTCC TCCTTCTCCT 
CCTCCACCAT CATCACCACC ACAAGGGCAG ACAGTGCGCC CAGGGGTCAA AGAGTCTCTT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn4a ( XM_006531988.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532051.1 Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X2, mRNA.

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CloneID	OMu09887
Clone ID Related Accession (Same CDS sequence)	XM_006531987.2 , XM_006531988.2 , NM_133199.2 , XM_006531987.3
Accession Version	XM_006531988.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	sodium channel protein type 4 subunit alpha isoform X1
Comment

ATGGCCAGCT CATCTCTGCC CACCCTGGTC CCCCCTGGTC CCCACTGCCT GCGCCCCTTC 
ACCCCAGAGT CCCTGGCAGC CATAGAACAG CGGGCAATGG AGGAGGAAGC CCGGCTGCAG 
CGGAACAAGC AAATGGAGAT TGAAGAGCCT GAGCGGAAGC CACGCAGTGA CCTGGAAGCT 
GGCAAGAACC TCCCACTCAT CTATGGGGAC CCCCCACCTG AAGTCATTGG CGTCCCCCTG 
GAGGACCTGG ACCCTTACTA CAGTGACAAG AAGACCTTCA TTGTGCTCAA CAAAGGAAAG 
GCCATCTTCC GATTCTCTGC CACCCCTGCC CTCTACATGC TGAGCCCCTT CAGCATCGTC 
CGGCGGGTGG CTATCAAGGT GCTCATCCAC GCGCTGTTCA GCATGTTCAT CATGATCACC 
ATCTTGACCA ACTGCGTGTT CATGACCATG AGCAACCCGC CTTCTTGGTC CAAGGACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTT GAGTCCCTCA TTAAGATGCT GGCCCGAGGC 
TTTTGCATTG ATGACTTCAC ATTCCTCCGA GACCCCTGGA ACTGGCTGGA CTTCAGTGTC 
ATCACAATGG CGTACGTGAC AGAGTTTGTG GACTTGGGCA ACATCTCAGC CCTGAGGACC 
TTCCGTGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACGATCGTG 
GGAGCCCTGA TCCAGTCTGT GAAAAAGCTC TCGGATGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTTTTCATGG GAAACCTGCG TCAGAAGTGC 
GTGCGTTGGC CCCCGCCCAT GAATGACACC AACACCACGT GGTATGGTAA TGATACGTGG 
TACGGCAATG ACACCTGGTA CGGCAATGAC ACCTGGTATG GCAATGACAC TTGGAACAGC 
CAGGAGAGCT GGGTCAGCAA CTCTACCTTT GACTGGGAGG CCTACATCAA TGACGAAGGG 
AACTTCTATT TCTTGGAGGG CTCCAATGAT GCCCTGCTCT GTGGGAATAG CAGTGATGCC 
GGGCACTGCC CTGAGGGCTA CGAATGCATG AAGGCTGGGA GGAACCCCAA CTATGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACGCAGGAC 
TACTGGGAGA ACCTTTTCCA GCTGACCCTT CGAGCCGCTG GCAAGACCTA CATGATCTTC 
TTTGTGGTCA TCATCTTCCT GGGTTCCTTC TACCTCATCA ATCTGATCCT GGCGGTGGTG 
GCCATGGCAT ATGCTGAGCA GAATGAGGCT ACCCTGGCCG AAGACCAGGA GAAAGAGGAG 
GAGTTCCAAC AGATGCTTGA AAAATTCAAA AAACATCAGG AGGAACTGGA AAAGGCGAAG 
GCTGCCCAGG CTCTGGAAGG TGGAGAGGAG GCAGATGGGG ACCCAACCCA CAGCAAAGAC 
TGCAATGGTA GCCTGGATAC ATCCGGGGAG AAGGGGCCCC CAAGGCCAAG CTGCAGCGCA 
GAGAGTGCCA TCTCAGATGC TATGGAGGAG CTGGAAGAGG CCCATCAGAA GTGCCCCCCA 
TGGTGGTACA AGTGTGCACA CAAAGTGCTC ATCTGGAACT GCTGTGCCCC GTGGGTGAAG 
TTCAAGCATA TTATCCTCCT GATTGTCATG GACCCCTTTG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCACT ACCCCATGAC TGAGCACTTT 
GACAACGTGC TCTCTGTGGG CAACTTGGTC TTCACGGGCA TCTTCACTGC AGAGATGGTG 
CTGAAGTTGA TTGCTATGGA CCCCTACGAA TATTTCCAAC AGGGCTGGAA CATCTTCGAC 
AGCTTCATCG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAACGTGCA GGGCCTATCA 
GTGCTCCGTT CTTTCCGTCT GCTGCGTGTC TTCAAGCTGG CCAAGTCATG GCCAACACTC 
AACATGCTCA TCAAAATCAT TGGCAACTCA GTGGGTGCGC TGGGCAACCT GACCCTGGTG 
TTGGCCATCA TCGTCTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAT 
AAGGAATGCG TGTGCAAGAT TGCCTCAGAC TGCAGCCTGC CCCGCTGGCA CATGCACGAT 
TTCTTCCACT CCTTCCTCAT CGTCTTCCGC ATCCTCTGTG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAAGTGGC CGGCCAGGCC ATGTGCCTCA CCGTCTTCCT CATGGTCATG 
GTCATTGGCA ACCTGGTGGT CCTGAATCTG TTCCTGGCTC TCCTGTTGAG TTCCTTCAGC 
GCTGACAGTC TGGCAGCGTC AGATGAAGAT GGTGAGATGA ACAACCTACA GATCGCCATC 
GGGCGTATCA AGTGGGGCAT CGCCTTTGCC AAAACCTTCC TCTTGGGGCT GTTGCATGGC 
AAGATCCTGA GCCTCAAAGA CATAATGCTC AGTCTCGGGG AGCCTGGGGG TGCTGGGGAA 
AACGGGGAGA GCCCTCCCGA GGATGAGAAG AAGGAACCAC CTCCGGAAGA TGGGAACAAG 
GAGCTGAAAG ACAATCATAT CCTGAACCAC GTGGGCCTGA CTGATGGACC CCGCTCCAGC 
ATCGAGATGG ACCACCTTAA CTTCATCAAC AACCCCTACC TCACTATCCA TGTGCCCATT 
GCCTCCGAGG AGTCCGACTT GGAAATGCCC ACAGAGGAGG AGACTGACAC CTTCTCGGAG 
CCTGAGGACA TCAAGAAGCC ACTACAGCCT CTGTACGACG GGAACTCCTC CGTCTGCAGC 
ACAGCTGACT ACAAGCCCCC CGAAGAGGAC CCTGAGGAGC AGGCTGAGGA GAACCCTGAG 
GGGGAGCTAC CTGAGGAGTG TTTCACAGAG GCCTGTGTGA AGCGCTGCCC CTGCCTCTAT 
GTGGACATCT CCCAGGGCCG CGGGAAAATG TGGTGGACAC TCCGCAGGGC CTGCTTCAAG 
ATTGTTGAGC ACAACTGGTT TGAGACCTTC ATCGTCTTCA TGATCTTGCT CAGCAGCGGG 
GCCCTGGCCT TCGAGGACAT CTACATTGAA CAGCGGCGAG TCATCCAAAC CATCCTGGAA 
TATGCTGACA AGGTCTTCAC CTACATCTTC ATCCTGGAGA TGCTGCTAAA GTGGGTGGCC 
TACGGCTTCA AGGTGTACTT CACCAACGCC TGGTGCTGGC TCGACTTCCT CATTGTGGAT 
GTCTCCATCA TCAGTCTGGT GGCTAACTGG CTGGGCTACT CTGAGCTGGG GCCCATCAAA 
TCCCTGCGCA CGCTCCGGGC CCTGCGACCT CTGAGGGCAC TGTCCCGATT TGAAGGCATG 
AGGGTGGTGG TGAACGCCCT TCTGGGAGCC ATCCCTTCCA TCATGAACGT GCTCCTTGTC 
TGCCTCATCT TCTGGCTCAT CTTCAGCATC ATGGGGGTCA ACTTGTTTGC TGGAAAGTTC 
TACTACTGCA TCAACACAAC CACCTCCGAG AGATTCGACA TCTCTGTGGT CAACAACAAG 
TCCGAGTGTG AGAGCCTCAT GTACACCGGC CAGGTCCGCT GGATGAACGT CAAGGTCAAC 
TATGACAACG TGGGTCTCGG CTACCTCTCT CTTCTTCAGG TGGCCACTTT CAAGGGTTGG 
ATGGACATCA TGTATGCAGC TGTGGACTCC CGGGAGAAAG AGGAGCAGCC AGACTACGAG 
GTGAACCTCT ACATGTACCT TTACTTCGTC ATCTTTATCA TCTTCGGCTC CTTCTTCACC 
CTCAATCTCT TCATTGGCGT CATCATTGAC AACTTCAACC AACAGAAGAA GAAGTTTGGA 
GGGAAAGACA TCTTCATGAC AGAGGAACAG AAGAAATACT ACAATGCGAT GAAGAAGCTT 
GGCTCCAAGA AGCCCCAGAA GCCAATCCCC CGGCCTCAGA ACAAGATCCA AGGCATGGTG 
TACGACTTCG TGACAAAGCA GGTGTTTGAC ATCTCTATCA TGATCCTCAT CTGCCTCAAC 
ATGGTGACGA TGATGGTGGA GACGGATGAC CAGAGCCAGC TCAAGGTGGA CATCCTGTAT 
AACATCAACA TGGTCTTCAT TATCGTCTTC ACAGGGGAGT GTGTGCTCAA GATGTTTGCC 
CTGCGCCATT ACTACTTCAC CATTGGCTGG AATATCTTCG ACTTCGTGGT TGTCATCTTG 
TCCATTGTGG GTCTCGCACT CTCTGACTTG ATACAGAAAT ACTTTGTGTC ACCCACACTG 
TTCCGTGTGA TCCGATTGGC TCGGATCGGG CGTGTCTTGC GTCTGATCCG TGGGGCCAAG 
GGCATCCGGA CGCTGCTGTT CGCCCTGATG ATGTCCCTGC CCGCCCTCTT CAACATCGGC 
CTCCTTCTCT TTCTGGTCAT GTTCATTTAC TCCATCTTCG GCATGTCTAA CTTCGCCTAC 
GTCAAGAAAG AGTCGGGCAT CGACGACATG TTCAACTTTG AGACCTTTGG CAACAGTATC 
ATCTGCCTCT TCGAGATCAC CACGTCAGCT GGCTGGGACG GGCTTCTGAA CCCCATCCTC 
AACAGTGGGC CCCCAGACTG TGACCCGACA TTGGAGAACC CAGGCACCAA TATCAAGGGT 
GACTGCGGCA ACCCATCCAT CGGCATCTGT TTCTTCTGCA GCTACATCAT CATCTCCTTC 
CTCATCGTGG TCAACATGTA CATTGCCATC ATCCTAGAGA ATTTCAATGT GGCCACTGAA 
GAGAGCAGTG AGCCCCTCTG TGAGGATGAC TTCGAGATGT TCTATGAGAC CTGGGAGAAG 
TTTGACCCCG ATGCAACTCA GTTCATCGAC TACAGCCGCC TTTCTGACTT TGTGGACACC 
CTGCAGGAGC CCTTGAAAAT TGCCAAGCCC AATAAGATCA AGCTTATCAC ATTAGACCTG 
CCCATGGTAC CCGGGGACAA GATCCACTGC CTGGACATCC TCTTTGCCCT GACCAAAGAG 
GTACTGGGTG ACTCTGGGGA AATGGATGCT CTCAAACAGA CCATGGAGGA GAAGTTCATG 
GCAGCTAACC CCTCCAAGGT CTCCTATGAG CCCATCACCA CCACCCTCAA GAGGAAGCAG 
GAGGAGGTGT GTGCTATCAA AATCCAGAGG GCTTACCGCC GCCATCTGCT GCAGCGCTCT 
GTGAAGCAGG CCTCTTACAT GTACCGCCAC AGCCAGGAAG GCAATGGCGA TGGGGCCCCT 
GAGAAGGAGG GATTGCTTGC CAACACCATG AACAAGATGT ATGGCTCTGA GAAAGAGGAC 
AACGGTGTGC AGAGCCAGGG GGAGAAAGAG AAAGACTCAA CAGAGGATGC TGGACCCACC 
ACGGAGGTCA CAGCCCCCAG CAGCTCGGAC ACTGCCCTGA CTCCTCCTCC TCCTTCTCCT 
CCTCCACCAT CATCACCACC ACAAGGGCAG ACAGTGCGCC CAGGGGTCAA AGAGTCTCTT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006532051.1

Translation

MASSSLPTLVPPGPHCLRPFTPESLAAIEQRAMEEEARLQRNKQMEIEEPERKPRSDLEAGKNLPLIYGDPPPEVIGVPLEDLDPYYSDKKTFIVLNKGKAIFRFSATPALYMLSPFSIVRRVAIKVLIHALFSMFIMITILTNCVFMTMSNPPSWSKDVEYTFTGIYTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVITMAYVTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPMNDTNTTWYGNDTWYGNDTWYGNDTWYGNDTWNSQESWVSNSTFDWEAYINDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECMKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKHQEELEKAKAAQALEGGEEADGDPTHSKDCNGSLDTSGEKGPPRPSCSAESAISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWVKFKHIILLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLSVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSFIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCSLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIGRIKWGIAFAKTFLLGLLHGKILSLKDIMLSLGEPGGAGENGESPPEDEKKEPPPEDGNKELKDNHILNHVGLTDGPRSSIEMDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDIKKPLQPLYDGNSSVCSTADYKPPEEDPEEQAEENPEGELPEECFTEACVKRCPCLYVDISQGRGKMWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRRVIQTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDISVVNNKSECESLMYTGQVRWMNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPDYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKIQGMVYDFVTKQVFDISIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIVFTGECVLKMFALRHYYFTIGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPTLENPGTNIKGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLCEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLKIAKPNKIKLITLDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKQTMEEKFMAANPSKVSYEPITTTLKRKQEEVCAIKIQRAYRRHLLQRSVKQASYMYRHSQEGNGDGAPEKEGLLANTMNKMYGSEKEDNGVQSQGEKEKDSTEDAGPTTEVTAPSSSDTALTPPPPSPPPPSSPPQGQTVRPGVKESLV

Target ORF information:

RefSeq Version	XM_006531988.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531988.2

ORF Insert Sequence:

ATGGCCAGCT CATCTCTGCC CACCCTGGTC CCCCCTGGTC CCCACTGCCT GCGCCCCTTC 
ACCCCAGAGT CCCTGGCAGC CATAGAACAG CGGGCAATGG AGGAGGAAGC CCGGCTGCAG 
CGGAACAAGC AAATGGAGAT TGAAGAGCCT GAGCGGAAGC CACGCAGTGA CCTGGAAGCT 
GGCAAGAACC TCCCACTCAT CTATGGGGAC CCCCCACCTG AAGTCATTGG CGTCCCCCTG 
GAGGACCTGG ACCCTTACTA CAGTGACAAG AAGACCTTCA TTGTGCTCAA CAAAGGAAAG 
GCCATCTTCC GATTCTCTGC CACCCCTGCC CTCTACATGC TGAGCCCCTT CAGCATCGTC 
CGGCGGGTGG CTATCAAGGT GCTCATCCAC GCGCTGTTCA GCATGTTCAT CATGATCACC 
ATCTTGACCA ACTGCGTGTT CATGACCATG AGCAACCCGC CTTCTTGGTC CAAGGACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTT GAGTCCCTCA TTAAGATGCT GGCCCGAGGC 
TTTTGCATTG ATGACTTCAC ATTCCTCCGA GACCCCTGGA ACTGGCTGGA CTTCAGTGTC 
ATCACAATGG CGTACGTGAC AGAGTTTGTG GACTTGGGCA ACATCTCAGC CCTGAGGACC 
TTCCGTGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACGATCGTG 
GGAGCCCTGA TCCAGTCTGT GAAAAAGCTC TCGGATGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTTTTCATGG GAAACCTGCG TCAGAAGTGC 
GTGCGTTGGC CCCCGCCCAT GAATGACACC AACACCACGT GGTATGGTAA TGATACGTGG 
TACGGCAATG ACACCTGGTA CGGCAATGAC ACCTGGTATG GCAATGACAC TTGGAACAGC 
CAGGAGAGCT GGGTCAGCAA CTCTACCTTT GACTGGGAGG CCTACATCAA TGACGAAGGG 
AACTTCTATT TCTTGGAGGG CTCCAATGAT GCCCTGCTCT GTGGGAATAG CAGTGATGCC 
GGGCACTGCC CTGAGGGCTA CGAATGCATG AAGGCTGGGA GGAACCCCAA CTATGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACGCAGGAC 
TACTGGGAGA ACCTTTTCCA GCTGACCCTT CGAGCCGCTG GCAAGACCTA CATGATCTTC 
TTTGTGGTCA TCATCTTCCT GGGTTCCTTC TACCTCATCA ATCTGATCCT GGCGGTGGTG 
GCCATGGCAT ATGCTGAGCA GAATGAGGCT ACCCTGGCCG AAGACCAGGA GAAAGAGGAG 
GAGTTCCAAC AGATGCTTGA AAAATTCAAA AAACATCAGG AGGAACTGGA AAAGGCGAAG 
GCTGCCCAGG CTCTGGAAGG TGGAGAGGAG GCAGATGGGG ACCCAACCCA CAGCAAAGAC 
TGCAATGGTA GCCTGGATAC ATCCGGGGAG AAGGGGCCCC CAAGGCCAAG CTGCAGCGCA 
GAGAGTGCCA TCTCAGATGC TATGGAGGAG CTGGAAGAGG CCCATCAGAA GTGCCCCCCA 
TGGTGGTACA AGTGTGCACA CAAAGTGCTC ATCTGGAACT GCTGTGCCCC GTGGGTGAAG 
TTCAAGCATA TTATCCTCCT GATTGTCATG GACCCCTTTG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCACT ACCCCATGAC TGAGCACTTT 
GACAACGTGC TCTCTGTGGG CAACTTGGTC TTCACGGGCA TCTTCACTGC AGAGATGGTG 
CTGAAGTTGA TTGCTATGGA CCCCTACGAA TATTTCCAAC AGGGCTGGAA CATCTTCGAC 
AGCTTCATCG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAACGTGCA GGGCCTATCA 
GTGCTCCGTT CTTTCCGTCT GCTGCGTGTC TTCAAGCTGG CCAAGTCATG GCCAACACTC 
AACATGCTCA TCAAAATCAT TGGCAACTCA GTGGGTGCGC TGGGCAACCT GACCCTGGTG 
TTGGCCATCA TCGTCTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAT 
AAGGAATGCG TGTGCAAGAT TGCCTCAGAC TGCAGCCTGC CCCGCTGGCA CATGCACGAT 
TTCTTCCACT CCTTCCTCAT CGTCTTCCGC ATCCTCTGTG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAAGTGGC CGGCCAGGCC ATGTGCCTCA CCGTCTTCCT CATGGTCATG 
GTCATTGGCA ACCTGGTGGT CCTGAATCTG TTCCTGGCTC TCCTGTTGAG TTCCTTCAGC 
GCTGACAGTC TGGCAGCGTC AGATGAAGAT GGTGAGATGA ACAACCTACA GATCGCCATC 
GGGCGTATCA AGTGGGGCAT CGCCTTTGCC AAAACCTTCC TCTTGGGGCT GTTGCATGGC 
AAGATCCTGA GCCTCAAAGA CATAATGCTC AGTCTCGGGG AGCCTGGGGG TGCTGGGGAA 
AACGGGGAGA GCCCTCCCGA GGATGAGAAG AAGGAACCAC CTCCGGAAGA TGGGAACAAG 
GAGCTGAAAG ACAATCATAT CCTGAACCAC GTGGGCCTGA CTGATGGACC CCGCTCCAGC 
ATCGAGATGG ACCACCTTAA CTTCATCAAC AACCCCTACC TCACTATCCA TGTGCCCATT 
GCCTCCGAGG AGTCCGACTT GGAAATGCCC ACAGAGGAGG AGACTGACAC CTTCTCGGAG 
CCTGAGGACA TCAAGAAGCC ACTACAGCCT CTGTACGACG GGAACTCCTC CGTCTGCAGC 
ACAGCTGACT ACAAGCCCCC CGAAGAGGAC CCTGAGGAGC AGGCTGAGGA GAACCCTGAG 
GGGGAGCTAC CTGAGGAGTG TTTCACAGAG GCCTGTGTGA AGCGCTGCCC CTGCCTCTAT 
GTGGACATCT CCCAGGGCCG CGGGAAAATG TGGTGGACAC TCCGCAGGGC CTGCTTCAAG 
ATTGTTGAGC ACAACTGGTT TGAGACCTTC ATCGTCTTCA TGATCTTGCT CAGCAGCGGG 
GCCCTGGCCT TCGAGGACAT CTACATTGAA CAGCGGCGAG TCATCCAAAC CATCCTGGAA 
TATGCTGACA AGGTCTTCAC CTACATCTTC ATCCTGGAGA TGCTGCTAAA GTGGGTGGCC 
TACGGCTTCA AGGTGTACTT CACCAACGCC TGGTGCTGGC TCGACTTCCT CATTGTGGAT 
GTCTCCATCA TCAGTCTGGT GGCTAACTGG CTGGGCTACT CTGAGCTGGG GCCCATCAAA 
TCCCTGCGCA CGCTCCGGGC CCTGCGACCT CTGAGGGCAC TGTCCCGATT TGAAGGCATG 
AGGGTGGTGG TGAACGCCCT TCTGGGAGCC ATCCCTTCCA TCATGAACGT GCTCCTTGTC 
TGCCTCATCT TCTGGCTCAT CTTCAGCATC ATGGGGGTCA ACTTGTTTGC TGGAAAGTTC 
TACTACTGCA TCAACACAAC CACCTCCGAG AGATTCGACA TCTCTGTGGT CAACAACAAG 
TCCGAGTGTG AGAGCCTCAT GTACACCGGC CAGGTCCGCT GGATGAACGT CAAGGTCAAC 
TATGACAACG TGGGTCTCGG CTACCTCTCT CTTCTTCAGG TGGCCACTTT CAAGGGTTGG 
ATGGACATCA TGTATGCAGC TGTGGACTCC CGGGAGAAAG AGGAGCAGCC AGACTACGAG 
GTGAACCTCT ACATGTACCT TTACTTCGTC ATCTTTATCA TCTTCGGCTC CTTCTTCACC 
CTCAATCTCT TCATTGGCGT CATCATTGAC AACTTCAACC AACAGAAGAA GAAGTTTGGA 
GGGAAAGACA TCTTCATGAC AGAGGAACAG AAGAAATACT ACAATGCGAT GAAGAAGCTT 
GGCTCCAAGA AGCCCCAGAA GCCAATCCCC CGGCCTCAGA ACAAGATCCA AGGCATGGTG 
TACGACTTCG TGACAAAGCA GGTGTTTGAC ATCTCTATCA TGATCCTCAT CTGCCTCAAC 
ATGGTGACGA TGATGGTGGA GACGGATGAC CAGAGCCAGC TCAAGGTGGA CATCCTGTAT 
AACATCAACA TGGTCTTCAT TATCGTCTTC ACAGGGGAGT GTGTGCTCAA GATGTTTGCC 
CTGCGCCATT ACTACTTCAC CATTGGCTGG AATATCTTCG ACTTCGTGGT TGTCATCTTG 
TCCATTGTGG GTCTCGCACT CTCTGACTTG ATACAGAAAT ACTTTGTGTC ACCCACACTG 
TTCCGTGTGA TCCGATTGGC TCGGATCGGG CGTGTCTTGC GTCTGATCCG TGGGGCCAAG 
GGCATCCGGA CGCTGCTGTT CGCCCTGATG ATGTCCCTGC CCGCCCTCTT CAACATCGGC 
CTCCTTCTCT TTCTGGTCAT GTTCATTTAC TCCATCTTCG GCATGTCTAA CTTCGCCTAC 
GTCAAGAAAG AGTCGGGCAT CGACGACATG TTCAACTTTG AGACCTTTGG CAACAGTATC 
ATCTGCCTCT TCGAGATCAC CACGTCAGCT GGCTGGGACG GGCTTCTGAA CCCCATCCTC 
AACAGTGGGC CCCCAGACTG TGACCCGACA TTGGAGAACC CAGGCACCAA TATCAAGGGT 
GACTGCGGCA ACCCATCCAT CGGCATCTGT TTCTTCTGCA GCTACATCAT CATCTCCTTC 
CTCATCGTGG TCAACATGTA CATTGCCATC ATCCTAGAGA ATTTCAATGT GGCCACTGAA 
GAGAGCAGTG AGCCCCTCTG TGAGGATGAC TTCGAGATGT TCTATGAGAC CTGGGAGAAG 
TTTGACCCCG ATGCAACTCA GTTCATCGAC TACAGCCGCC TTTCTGACTT TGTGGACACC 
CTGCAGGAGC CCTTGAAAAT TGCCAAGCCC AATAAGATCA AGCTTATCAC ATTAGACCTG 
CCCATGGTAC CCGGGGACAA GATCCACTGC CTGGACATCC TCTTTGCCCT GACCAAAGAG 
GTACTGGGTG ACTCTGGGGA AATGGATGCT CTCAAACAGA CCATGGAGGA GAAGTTCATG 
GCAGCTAACC CCTCCAAGGT CTCCTATGAG CCCATCACCA CCACCCTCAA GAGGAAGCAG 
GAGGAGGTGT GTGCTATCAA AATCCAGAGG GCTTACCGCC GCCATCTGCT GCAGCGCTCT 
GTGAAGCAGG CCTCTTACAT GTACCGCCAC AGCCAGGAAG GCAATGGCGA TGGGGCCCCT 
GAGAAGGAGG GATTGCTTGC CAACACCATG AACAAGATGT ATGGCTCTGA GAAAGAGGAC 
AACGGTGTGC AGAGCCAGGG GGAGAAAGAG AAAGACTCAA CAGAGGATGC TGGACCCACC 
ACGGAGGTCA CAGCCCCCAG CAGCTCGGAC ACTGCCCTGA CTCCTCCTCC TCCTTCTCCT 
CCTCCACCAT CATCACCACC ACAAGGGCAG ACAGTGCGCC CAGGGGTCAA AGAGTCTCTT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn4a ( XM_006531987.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532050.1 Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X2, mRNA.

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CloneID	OMu09887
Clone ID Related Accession (Same CDS sequence)	XM_006531987.2 , XM_006531988.2 , NM_133199.2 , XM_006531987.3
Accession Version	XM_006531987.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sodium channel protein type 4 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531987.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CATCTCTGCC CACCCTGGTC CCCCCTGGTC CCCACTGCCT GCGCCCCTTC 
ACCCCAGAGT CCCTGGCAGC CATAGAACAG CGGGCAATGG AGGAGGAAGC CCGGCTGCAG 
CGGAACAAGC AAATGGAGAT TGAAGAGCCT GAGCGGAAGC CACGCAGTGA CCTGGAAGCT 
GGCAAGAACC TCCCACTCAT CTATGGGGAC CCCCCACCTG AAGTCATTGG CGTCCCCCTG 
GAGGACCTGG ACCCTTACTA CAGTGACAAG AAGACCTTCA TTGTGCTCAA CAAAGGAAAG 
GCCATCTTCC GATTCTCTGC CACCCCTGCC CTCTACATGC TGAGCCCCTT CAGCATCGTC 
CGGCGGGTGG CTATCAAGGT GCTCATCCAC GCGCTGTTCA GCATGTTCAT CATGATCACC 
ATCTTGACCA ACTGCGTGTT CATGACCATG AGCAACCCGC CTTCTTGGTC CAAGGACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTT GAGTCCCTCA TTAAGATGCT GGCCCGAGGC 
TTTTGCATTG ATGACTTCAC ATTCCTCCGA GACCCCTGGA ACTGGCTGGA CTTCAGTGTC 
ATCACAATGG CGTACGTGAC AGAGTTTGTG GACTTGGGCA ACATCTCAGC CCTGAGGACC 
TTCCGTGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACGATCGTG 
GGAGCCCTGA TCCAGTCTGT GAAAAAGCTC TCGGATGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTTTTCATGG GAAACCTGCG TCAGAAGTGC 
GTGCGTTGGC CCCCGCCCAT GAATGACACC AACACCACGT GGTATGGTAA TGATACGTGG 
TACGGCAATG ACACCTGGTA CGGCAATGAC ACCTGGTATG GCAATGACAC TTGGAACAGC 
CAGGAGAGCT GGGTCAGCAA CTCTACCTTT GACTGGGAGG CCTACATCAA TGACGAAGGG 
AACTTCTATT TCTTGGAGGG CTCCAATGAT GCCCTGCTCT GTGGGAATAG CAGTGATGCC 
GGGCACTGCC CTGAGGGCTA CGAATGCATG AAGGCTGGGA GGAACCCCAA CTATGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACGCAGGAC 
TACTGGGAGA ACCTTTTCCA GCTGACCCTT CGAGCCGCTG GCAAGACCTA CATGATCTTC 
TTTGTGGTCA TCATCTTCCT GGGTTCCTTC TACCTCATCA ATCTGATCCT GGCGGTGGTG 
GCCATGGCAT ATGCTGAGCA GAATGAGGCT ACCCTGGCCG AAGACCAGGA GAAAGAGGAG 
GAGTTCCAAC AGATGCTTGA AAAATTCAAA AAACATCAGG AGGAACTGGA AAAGGCGAAG 
GCTGCCCAGG CTCTGGAAGG TGGAGAGGAG GCAGATGGGG ACCCAACCCA CAGCAAAGAC 
TGCAATGGTA GCCTGGATAC ATCCGGGGAG AAGGGGCCCC CAAGGCCAAG CTGCAGCGCA 
GAGAGTGCCA TCTCAGATGC TATGGAGGAG CTGGAAGAGG CCCATCAGAA GTGCCCCCCA 
TGGTGGTACA AGTGTGCACA CAAAGTGCTC ATCTGGAACT GCTGTGCCCC GTGGGTGAAG 
TTCAAGCATA TTATCCTCCT GATTGTCATG GACCCCTTTG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCACT ACCCCATGAC TGAGCACTTT 
GACAACGTGC TCTCTGTGGG CAACTTGGTC TTCACGGGCA TCTTCACTGC AGAGATGGTG 
CTGAAGTTGA TTGCTATGGA CCCCTACGAA TATTTCCAAC AGGGCTGGAA CATCTTCGAC 
AGCTTCATCG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAACGTGCA GGGCCTATCA 
GTGCTCCGTT CTTTCCGTCT GCTGCGTGTC TTCAAGCTGG CCAAGTCATG GCCAACACTC 
AACATGCTCA TCAAAATCAT TGGCAACTCA GTGGGTGCGC TGGGCAACCT GACCCTGGTG 
TTGGCCATCA TCGTCTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAT 
AAGGAATGCG TGTGCAAGAT TGCCTCAGAC TGCAGCCTGC CCCGCTGGCA CATGCACGAT 
TTCTTCCACT CCTTCCTCAT CGTCTTCCGC ATCCTCTGTG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAAGTGGC CGGCCAGGCC ATGTGCCTCA CCGTCTTCCT CATGGTCATG 
GTCATTGGCA ACCTGGTGGT CCTGAATCTG TTCCTGGCTC TCCTGTTGAG TTCCTTCAGC 
GCTGACAGTC TGGCAGCGTC AGATGAAGAT GGTGAGATGA ACAACCTACA GATCGCCATC 
GGGCGTATCA AGTGGGGCAT CGCCTTTGCC AAAACCTTCC TCTTGGGGCT GTTGCATGGC 
AAGATCCTGA GCCTCAAAGA CATAATGCTC AGTCTCGGGG AGCCTGGGGG TGCTGGGGAA 
AACGGGGAGA GCCCTCCCGA GGATGAGAAG AAGGAACCAC CTCCGGAAGA TGGGAACAAG 
GAGCTGAAAG ACAATCATAT CCTGAACCAC GTGGGCCTGA CTGATGGACC CCGCTCCAGC 
ATCGAGATGG ACCACCTTAA CTTCATCAAC AACCCCTACC TCACTATCCA TGTGCCCATT 
GCCTCCGAGG AGTCCGACTT GGAAATGCCC ACAGAGGAGG AGACTGACAC CTTCTCGGAG 
CCTGAGGACA TCAAGAAGCC ACTACAGCCT CTGTACGACG GGAACTCCTC CGTCTGCAGC 
ACAGCTGACT ACAAGCCCCC CGAAGAGGAC CCTGAGGAGC AGGCTGAGGA GAACCCTGAG 
GGGGAGCTAC CTGAGGAGTG TTTCACAGAG GCCTGTGTGA AGCGCTGCCC CTGCCTCTAT 
GTGGACATCT CCCAGGGCCG CGGGAAAATG TGGTGGACAC TCCGCAGGGC CTGCTTCAAG 
ATTGTTGAGC ACAACTGGTT TGAGACCTTC ATCGTCTTCA TGATCTTGCT CAGCAGCGGG 
GCCCTGGCCT TCGAGGACAT CTACATTGAA CAGCGGCGAG TCATCCAAAC CATCCTGGAA 
TATGCTGACA AGGTCTTCAC CTACATCTTC ATCCTGGAGA TGCTGCTAAA GTGGGTGGCC 
TACGGCTTCA AGGTGTACTT CACCAACGCC TGGTGCTGGC TCGACTTCCT CATTGTGGAT 
GTCTCCATCA TCAGTCTGGT GGCTAACTGG CTGGGCTACT CTGAGCTGGG GCCCATCAAA 
TCCCTGCGCA CGCTCCGGGC CCTGCGACCT CTGAGGGCAC TGTCCCGATT TGAAGGCATG 
AGGGTGGTGG TGAACGCCCT TCTGGGAGCC ATCCCTTCCA TCATGAACGT GCTCCTTGTC 
TGCCTCATCT TCTGGCTCAT CTTCAGCATC ATGGGGGTCA ACTTGTTTGC TGGAAAGTTC 
TACTACTGCA TCAACACAAC CACCTCCGAG AGATTCGACA TCTCTGTGGT CAACAACAAG 
TCCGAGTGTG AGAGCCTCAT GTACACCGGC CAGGTCCGCT GGATGAACGT CAAGGTCAAC 
TATGACAACG TGGGTCTCGG CTACCTCTCT CTTCTTCAGG TGGCCACTTT CAAGGGTTGG 
ATGGACATCA TGTATGCAGC TGTGGACTCC CGGGAGAAAG AGGAGCAGCC AGACTACGAG 
GTGAACCTCT ACATGTACCT TTACTTCGTC ATCTTTATCA TCTTCGGCTC CTTCTTCACC 
CTCAATCTCT TCATTGGCGT CATCATTGAC AACTTCAACC AACAGAAGAA GAAGTTTGGA 
GGGAAAGACA TCTTCATGAC AGAGGAACAG AAGAAATACT ACAATGCGAT GAAGAAGCTT 
GGCTCCAAGA AGCCCCAGAA GCCAATCCCC CGGCCTCAGA ACAAGATCCA AGGCATGGTG 
TACGACTTCG TGACAAAGCA GGTGTTTGAC ATCTCTATCA TGATCCTCAT CTGCCTCAAC 
ATGGTGACGA TGATGGTGGA GACGGATGAC CAGAGCCAGC TCAAGGTGGA CATCCTGTAT 
AACATCAACA TGGTCTTCAT TATCGTCTTC ACAGGGGAGT GTGTGCTCAA GATGTTTGCC 
CTGCGCCATT ACTACTTCAC CATTGGCTGG AATATCTTCG ACTTCGTGGT TGTCATCTTG 
TCCATTGTGG GTCTCGCACT CTCTGACTTG ATACAGAAAT ACTTTGTGTC ACCCACACTG 
TTCCGTGTGA TCCGATTGGC TCGGATCGGG CGTGTCTTGC GTCTGATCCG TGGGGCCAAG 
GGCATCCGGA CGCTGCTGTT CGCCCTGATG ATGTCCCTGC CCGCCCTCTT CAACATCGGC 
CTCCTTCTCT TTCTGGTCAT GTTCATTTAC TCCATCTTCG GCATGTCTAA CTTCGCCTAC 
GTCAAGAAAG AGTCGGGCAT CGACGACATG TTCAACTTTG AGACCTTTGG CAACAGTATC 
ATCTGCCTCT TCGAGATCAC CACGTCAGCT GGCTGGGACG GGCTTCTGAA CCCCATCCTC 
AACAGTGGGC CCCCAGACTG TGACCCGACA TTGGAGAACC CAGGCACCAA TATCAAGGGT 
GACTGCGGCA ACCCATCCAT CGGCATCTGT TTCTTCTGCA GCTACATCAT CATCTCCTTC 
CTCATCGTGG TCAACATGTA CATTGCCATC ATCCTAGAGA ATTTCAATGT GGCCACTGAA 
GAGAGCAGTG AGCCCCTCTG TGAGGATGAC TTCGAGATGT TCTATGAGAC CTGGGAGAAG 
TTTGACCCCG ATGCAACTCA GTTCATCGAC TACAGCCGCC TTTCTGACTT TGTGGACACC 
CTGCAGGAGC CCTTGAAAAT TGCCAAGCCC AATAAGATCA AGCTTATCAC ATTAGACCTG 
CCCATGGTAC CCGGGGACAA GATCCACTGC CTGGACATCC TCTTTGCCCT GACCAAAGAG 
GTACTGGGTG ACTCTGGGGA AATGGATGCT CTCAAACAGA CCATGGAGGA GAAGTTCATG 
GCAGCTAACC CCTCCAAGGT CTCCTATGAG CCCATCACCA CCACCCTCAA GAGGAAGCAG 
GAGGAGGTGT GTGCTATCAA AATCCAGAGG GCTTACCGCC GCCATCTGCT GCAGCGCTCT 
GTGAAGCAGG CCTCTTACAT GTACCGCCAC AGCCAGGAAG GCAATGGCGA TGGGGCCCCT 
GAGAAGGAGG GATTGCTTGC CAACACCATG AACAAGATGT ATGGCTCTGA GAAAGAGGAC 
AACGGTGTGC AGAGCCAGGG GGAGAAAGAG AAAGACTCAA CAGAGGATGC TGGACCCACC 
ACGGAGGTCA CAGCCCCCAG CAGCTCGGAC ACTGCCCTGA CTCCTCCTCC TCCTTCTCCT 
CCTCCACCAT CATCACCACC ACAAGGGCAG ACAGTGCGCC CAGGGGTCAA AGAGTCTCTT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532050.1
CDS	488..6013
Translation	MASSSLPTLVPPGPHCLRPFTPESLAAIEQRAMEEEARLQRNKQMEIEEPERKPRSDLEAGKNLPLIYGDPPPEVIGVPLEDLDPYYSDKKTFIVLNKGKAIFRFSATPALYMLSPFSIVRRVAIKVLIHALFSMFIMITILTNCVFMTMSNPPSWSKDVEYTFTGIYTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVITMAYVTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPMNDTNTTWYGNDTWYGNDTWYGNDTWYGNDTWNSQESWVSNSTFDWEAYINDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECMKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKHQEELEKAKAAQALEGGEEADGDPTHSKDCNGSLDTSGEKGPPRPSCSAESAISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWVKFKHIILLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLSVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSFIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIASDCSLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIGRIKWGIAFAKTFLLGLLHGKILSLKDIMLSLGEPGGAGENGESPPEDEKKEPPPEDGNKELKDNHILNHVGLTDGPRSSIEMDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDIKKPLQPLYDGNSSVCSTADYKPPEEDPEEQAEENPEGELPEECFTEACVKRCPCLYVDISQGRGKMWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRRVIQTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDISVVNNKSECESLMYTGQVRWMNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPDYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKIQGMVYDFVTKQVFDISIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIVFTGECVLKMFALRHYYFTIGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPTLENPGTNIKGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLCEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLKIAKPNKIKLITLDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKQTMEEKFMAANPSKVSYEPITTTLKRKQEEVCAIKIQRAYRRHLLQRSVKQASYMYRHSQEGNGDGAPEKEGLLANTMNKMYGSEKEDNGVQSQGEKEKDSTEDAGPTTEVTAPSSSDTALTPPPPSPPPPSSPPQGQTVRPGVKESLV

Target ORF information:

RefSeq Version	XM_006531987.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sodium channel, voltage-gated, type IV, alpha (Scn4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531987.3

ORF Insert Sequence:

ATGGCCAGCT CATCTCTGCC CACCCTGGTC CCCCCTGGTC CCCACTGCCT GCGCCCCTTC 
ACCCCAGAGT CCCTGGCAGC CATAGAACAG CGGGCAATGG AGGAGGAAGC CCGGCTGCAG 
CGGAACAAGC AAATGGAGAT TGAAGAGCCT GAGCGGAAGC CACGCAGTGA CCTGGAAGCT 
GGCAAGAACC TCCCACTCAT CTATGGGGAC CCCCCACCTG AAGTCATTGG CGTCCCCCTG 
GAGGACCTGG ACCCTTACTA CAGTGACAAG AAGACCTTCA TTGTGCTCAA CAAAGGAAAG 
GCCATCTTCC GATTCTCTGC CACCCCTGCC CTCTACATGC TGAGCCCCTT CAGCATCGTC 
CGGCGGGTGG CTATCAAGGT GCTCATCCAC GCGCTGTTCA GCATGTTCAT CATGATCACC 
ATCTTGACCA ACTGCGTGTT CATGACCATG AGCAACCCGC CTTCTTGGTC CAAGGACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTT GAGTCCCTCA TTAAGATGCT GGCCCGAGGC 
TTTTGCATTG ATGACTTCAC ATTCCTCCGA GACCCCTGGA ACTGGCTGGA CTTCAGTGTC 
ATCACAATGG CGTACGTGAC AGAGTTTGTG GACTTGGGCA ACATCTCAGC CCTGAGGACC 
TTCCGTGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACGATCGTG 
GGAGCCCTGA TCCAGTCTGT GAAAAAGCTC TCGGATGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTTTTCATGG GAAACCTGCG TCAGAAGTGC 
GTGCGTTGGC CCCCGCCCAT GAATGACACC AACACCACGT GGTATGGTAA TGATACGTGG 
TACGGCAATG ACACCTGGTA CGGCAATGAC ACCTGGTATG GCAATGACAC TTGGAACAGC 
CAGGAGAGCT GGGTCAGCAA CTCTACCTTT GACTGGGAGG CCTACATCAA TGACGAAGGG 
AACTTCTATT TCTTGGAGGG CTCCAATGAT GCCCTGCTCT GTGGGAATAG CAGTGATGCC 
GGGCACTGCC CTGAGGGCTA CGAATGCATG AAGGCTGGGA GGAACCCCAA CTATGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACGCAGGAC 
TACTGGGAGA ACCTTTTCCA GCTGACCCTT CGAGCCGCTG GCAAGACCTA CATGATCTTC 
TTTGTGGTCA TCATCTTCCT GGGTTCCTTC TACCTCATCA ATCTGATCCT GGCGGTGGTG 
GCCATGGCAT ATGCTGAGCA GAATGAGGCT ACCCTGGCCG AAGACCAGGA GAAAGAGGAG 
GAGTTCCAAC AGATGCTTGA AAAATTCAAA AAACATCAGG AGGAACTGGA AAAGGCGAAG 
GCTGCCCAGG CTCTGGAAGG TGGAGAGGAG GCAGATGGGG ACCCAACCCA CAGCAAAGAC 
TGCAATGGTA GCCTGGATAC ATCCGGGGAG AAGGGGCCCC CAAGGCCAAG CTGCAGCGCA 
GAGAGTGCCA TCTCAGATGC TATGGAGGAG CTGGAAGAGG CCCATCAGAA GTGCCCCCCA 
TGGTGGTACA AGTGTGCACA CAAAGTGCTC ATCTGGAACT GCTGTGCCCC GTGGGTGAAG 
TTCAAGCATA TTATCCTCCT GATTGTCATG GACCCCTTTG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCACT ACCCCATGAC TGAGCACTTT 
GACAACGTGC TCTCTGTGGG CAACTTGGTC TTCACGGGCA TCTTCACTGC AGAGATGGTG 
CTGAAGTTGA TTGCTATGGA CCCCTACGAA TATTTCCAAC AGGGCTGGAA CATCTTCGAC 
AGCTTCATCG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAACGTGCA GGGCCTATCA 
GTGCTCCGTT CTTTCCGTCT GCTGCGTGTC TTCAAGCTGG CCAAGTCATG GCCAACACTC 
AACATGCTCA TCAAAATCAT TGGCAACTCA GTGGGTGCGC TGGGCAACCT GACCCTGGTG 
TTGGCCATCA TCGTCTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAT 
AAGGAATGCG TGTGCAAGAT TGCCTCAGAC TGCAGCCTGC CCCGCTGGCA CATGCACGAT 
TTCTTCCACT CCTTCCTCAT CGTCTTCCGC ATCCTCTGTG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAAGTGGC CGGCCAGGCC ATGTGCCTCA CCGTCTTCCT CATGGTCATG 
GTCATTGGCA ACCTGGTGGT CCTGAATCTG TTCCTGGCTC TCCTGTTGAG TTCCTTCAGC 
GCTGACAGTC TGGCAGCGTC AGATGAAGAT GGTGAGATGA ACAACCTACA GATCGCCATC 
GGGCGTATCA AGTGGGGCAT CGCCTTTGCC AAAACCTTCC TCTTGGGGCT GTTGCATGGC 
AAGATCCTGA GCCTCAAAGA CATAATGCTC AGTCTCGGGG AGCCTGGGGG TGCTGGGGAA 
AACGGGGAGA GCCCTCCCGA GGATGAGAAG AAGGAACCAC CTCCGGAAGA TGGGAACAAG 
GAGCTGAAAG ACAATCATAT CCTGAACCAC GTGGGCCTGA CTGATGGACC CCGCTCCAGC 
ATCGAGATGG ACCACCTTAA CTTCATCAAC AACCCCTACC TCACTATCCA TGTGCCCATT 
GCCTCCGAGG AGTCCGACTT GGAAATGCCC ACAGAGGAGG AGACTGACAC CTTCTCGGAG 
CCTGAGGACA TCAAGAAGCC ACTACAGCCT CTGTACGACG GGAACTCCTC CGTCTGCAGC 
ACAGCTGACT ACAAGCCCCC CGAAGAGGAC CCTGAGGAGC AGGCTGAGGA GAACCCTGAG 
GGGGAGCTAC CTGAGGAGTG TTTCACAGAG GCCTGTGTGA AGCGCTGCCC CTGCCTCTAT 
GTGGACATCT CCCAGGGCCG CGGGAAAATG TGGTGGACAC TCCGCAGGGC CTGCTTCAAG 
ATTGTTGAGC ACAACTGGTT TGAGACCTTC ATCGTCTTCA TGATCTTGCT CAGCAGCGGG 
GCCCTGGCCT TCGAGGACAT CTACATTGAA CAGCGGCGAG TCATCCAAAC CATCCTGGAA 
TATGCTGACA AGGTCTTCAC CTACATCTTC ATCCTGGAGA TGCTGCTAAA GTGGGTGGCC 
TACGGCTTCA AGGTGTACTT CACCAACGCC TGGTGCTGGC TCGACTTCCT CATTGTGGAT 
GTCTCCATCA TCAGTCTGGT GGCTAACTGG CTGGGCTACT CTGAGCTGGG GCCCATCAAA 
TCCCTGCGCA CGCTCCGGGC CCTGCGACCT CTGAGGGCAC TGTCCCGATT TGAAGGCATG 
AGGGTGGTGG TGAACGCCCT TCTGGGAGCC ATCCCTTCCA TCATGAACGT GCTCCTTGTC 
TGCCTCATCT TCTGGCTCAT CTTCAGCATC ATGGGGGTCA ACTTGTTTGC TGGAAAGTTC 
TACTACTGCA TCAACACAAC CACCTCCGAG AGATTCGACA TCTCTGTGGT CAACAACAAG 
TCCGAGTGTG AGAGCCTCAT GTACACCGGC CAGGTCCGCT GGATGAACGT CAAGGTCAAC 
TATGACAACG TGGGTCTCGG CTACCTCTCT CTTCTTCAGG TGGCCACTTT CAAGGGTTGG 
ATGGACATCA TGTATGCAGC TGTGGACTCC CGGGAGAAAG AGGAGCAGCC AGACTACGAG 
GTGAACCTCT ACATGTACCT TTACTTCGTC ATCTTTATCA TCTTCGGCTC CTTCTTCACC 
CTCAATCTCT TCATTGGCGT CATCATTGAC AACTTCAACC AACAGAAGAA GAAGTTTGGA 
GGGAAAGACA TCTTCATGAC AGAGGAACAG AAGAAATACT ACAATGCGAT GAAGAAGCTT 
GGCTCCAAGA AGCCCCAGAA GCCAATCCCC CGGCCTCAGA ACAAGATCCA AGGCATGGTG 
TACGACTTCG TGACAAAGCA GGTGTTTGAC ATCTCTATCA TGATCCTCAT CTGCCTCAAC 
ATGGTGACGA TGATGGTGGA GACGGATGAC CAGAGCCAGC TCAAGGTGGA CATCCTGTAT 
AACATCAACA TGGTCTTCAT TATCGTCTTC ACAGGGGAGT GTGTGCTCAA GATGTTTGCC 
CTGCGCCATT ACTACTTCAC CATTGGCTGG AATATCTTCG ACTTCGTGGT TGTCATCTTG 
TCCATTGTGG GTCTCGCACT CTCTGACTTG ATACAGAAAT ACTTTGTGTC ACCCACACTG 
TTCCGTGTGA TCCGATTGGC TCGGATCGGG CGTGTCTTGC GTCTGATCCG TGGGGCCAAG 
GGCATCCGGA CGCTGCTGTT CGCCCTGATG ATGTCCCTGC CCGCCCTCTT CAACATCGGC 
CTCCTTCTCT TTCTGGTCAT GTTCATTTAC TCCATCTTCG GCATGTCTAA CTTCGCCTAC 
GTCAAGAAAG AGTCGGGCAT CGACGACATG TTCAACTTTG AGACCTTTGG CAACAGTATC 
ATCTGCCTCT TCGAGATCAC CACGTCAGCT GGCTGGGACG GGCTTCTGAA CCCCATCCTC 
AACAGTGGGC CCCCAGACTG TGACCCGACA TTGGAGAACC CAGGCACCAA TATCAAGGGT 
GACTGCGGCA ACCCATCCAT CGGCATCTGT TTCTTCTGCA GCTACATCAT CATCTCCTTC 
CTCATCGTGG TCAACATGTA CATTGCCATC ATCCTAGAGA ATTTCAATGT GGCCACTGAA 
GAGAGCAGTG AGCCCCTCTG TGAGGATGAC TTCGAGATGT TCTATGAGAC CTGGGAGAAG 
TTTGACCCCG ATGCAACTCA GTTCATCGAC TACAGCCGCC TTTCTGACTT TGTGGACACC 
CTGCAGGAGC CCTTGAAAAT TGCCAAGCCC AATAAGATCA AGCTTATCAC ATTAGACCTG 
CCCATGGTAC CCGGGGACAA GATCCACTGC CTGGACATCC TCTTTGCCCT GACCAAAGAG 
GTACTGGGTG ACTCTGGGGA AATGGATGCT CTCAAACAGA CCATGGAGGA GAAGTTCATG 
GCAGCTAACC CCTCCAAGGT CTCCTATGAG CCCATCACCA CCACCCTCAA GAGGAAGCAG 
GAGGAGGTGT GTGCTATCAA AATCCAGAGG GCTTACCGCC GCCATCTGCT GCAGCGCTCT 
GTGAAGCAGG CCTCTTACAT GTACCGCCAC AGCCAGGAAG GCAATGGCGA TGGGGCCCCT 
GAGAAGGAGG GATTGCTTGC CAACACCATG AACAAGATGT ATGGCTCTGA GAAAGAGGAC 
AACGGTGTGC AGAGCCAGGG GGAGAAAGAG AAAGACTCAA CAGAGGATGC TGGACCCACC 
ACGGAGGTCA CAGCCCCCAG CAGCTCGGAC ACTGCCCTGA CTCCTCCTCC TCCTTCTCCT 
CCTCCACCAT CATCACCACC ACAAGGGCAG ACAGTGCGCC CAGGGGTCAA AGAGTCTCTT 
GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Mice with an NaV1.4 sodium channel null allele have latent myasthenia, without susceptibility to periodic paralysis.
Brain : a journal of neurology139(Pt 6)1688-99(2016 Jun)
Wu F,Mi W,Fu Y,Struyk A,Cannon SC

Physiological basis for muscle stiffness and weakness in a knock-in M1592V mouse model of hyperkalemic periodic paralysis.
Physiological reports3(12)(2015 Dec)
Khogali S,Lucas B,Ammar T,Dejong D,Barbalinardo M,Hayward LJ,Renaud JM

Novel mutations in human and mouse SCN4A implicate AMPK in myotonia and periodic paralysis.
Brain : a journal of neurology137(Pt 12)3171-85(2014 Dec)
Corrochano S,M?nnikk? R,Joyce PI,McGoldrick P,Wettstein J,Lassi G,Raja Rayan DL,Blanco G,Quinn C,Liavas A,Lionikas A,Amior N,Dick J,Healy EG,Stewart M,Carter S,Hutchinson M,Bentley L,Fratta P,Cortese A,Cox R,Brown SD,Tucci V,Wackerhage H,Amato AA,Greensmith L,Koltzenburg M,Hanna MG,Acevedo-Arozena A

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Scn4a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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