vwa5b2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	vwa5b2
Entrez Gene ID	100488101
Full Name	von Willebrand factor A domain containing 5B2
General protein information	Preferred Names von Willebrand factor A domain containing 5B2 Names von Willebrand factor A domain-containing protein 5B2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018094413.2	XP_017949902.1	von Willebrand factor A domain-containing protein 5B2 isoform X1
XM_031902162.1	XP_031758022.1	von Willebrand factor A domain-containing protein 5B2 isoform X2
XM_002935141.4	XP_002935187.2	von Willebrand factor A domain-containing protein 5B2 isoform X1
XM_012963368.2	XP_012818822.1	von Willebrand factor A domain-containing protein 5B2 isoform X2
XM_012963369.2	XP_012818823.1	von Willebrand factor A domain-containing protein 5B2 isoform X2
XM_012963368.3	XP_012818822.1	von Willebrand factor A domain-containing protein 5B2 isoform X1
XM_012963369.3	XP_012818823.1	von Willebrand factor A domain-containing protein 5B2 isoform X1
XM_018094413.1	XP_017949902.1	von Willebrand factor A domain-containing protein 5B2 isoform X2

Pathways from BioSystems

Homologies

	VWA5B2	XP_001099634.2
Canis lupus familiaris (dog)	VWA5B2	XP_849568.2
Xenopus tropicalis (tropical clawed frog)	vwa5b2	XP_002935187.2
Homo sapiens (human)	VWA5B2	NP_612354.1
Rattus norvegicus (Norway rat)	Vwa5b2	NP_001128007.1
Gallus gallus (chicken)	VWA5B2	XP_422758.4
Danio rerio (zebrafish)	LOC101882427	XP_005171494.1
Pan troglodytes (chimpanzee)	VWA5B2	XP_003950295.1
Bos taurus (cattle)	VWA5B2	XP_002684922.1
Mus musculus (house mouse)	Vwa5b2	NP_001138425.1

Gene Ontology

The following vwa5b2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vwa5b2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa34064	XM_012963369.3 Latest version!	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa34064	XM_012963368.3 Latest version!	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa34064	XM_018094413.2 Latest version!	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa58471	XM_031902162.1 Latest version!	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa10198	XM_002935141.4 Latest version!	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OXa34064	XM_012963368.2	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa34064	XM_012963369.2	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa34064	XM_018094413.1	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vwa5b2 ( XM_018094413.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949902.1 Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa34064
Clone ID Related Accession (Same CDS sequence)	XM_018094413.2 , XM_012963368.2 , XM_012963369.2 , XM_012963368.3 , XM_012963369.3 , XM_018094413.1
Accession Version	XM_018094413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor A domain-containing protein 5B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGGT TGTGCTTATG TCCAACCAGC 
TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA AATCCATGGG CGTTGCTCAG TGAATCCCCT 
CCTCTGGTCC TGCCTTTACC CTCTGCAGGT TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT 
ACCAATGCAC AGCGTTATCA ATTCAGCTTT CGTCTCCAAG TACAAGGACC TTGTCTTCTG 
GCAGGAATAG AAAGTACTTC TCATGCTCTC CGTGCTGATG CTAGTCCATT TGCTGTGTCA 
GCCTCTTCTG TTTGGATCAG TTTGGCAGAA GAACATGACT GTAACCGAGC TTTGGAAATA 
ATCCTGCATC CTAGTGAACC ATACATTCCT CACTTATGTT TGGAGAGAGG CAAAAAAAGT 
TTCTCTCAAT ACAAAATGCA GATAAGAGAG AGAGGAGACT TCATAAGGGC AGTTAAGAAA 
TCTAGTGACC CAGAAAGACA GGTCAGCTTT GTAAGGAGAC GCTTCCACAA AGATCTCCAT 
TTTACCCCAG TCTTGATGTT GAACTTCTGC CCTGACCTTA CTGCCCTCAG CTCATTAATC 
TATGACCTCC CTACTATAAC CAGAGAAGTC ATTTTCCTTG TTTATCACTC AGGGGAGCTT 
TCGCATAATG TCCGGGATGC TCTCTTGCTG GCTGTTAGGA GTTTACCTGC ACGTACTTTG 
CTGAATCTGG GAAGTGGCAC ATGTGACAGC ATAACCCTGT TTCCGACCAG CAGGCAGTGT 
AGCAATGAGT CATTGGAGGC AGCTTATGAT CATATTAAGA ATGGACAACT CAGTTGCAGT 
TCAAGTAACT TGGTGACTGT TCTGGGCCAT ATCCTGTCTC TTCCAGTGCA TCGTGGATAC 
CCACGGCAGC TCTTTCTCTT CACTGACACA TCTCCAAGGT GTCCTGAAAG AGCCATTGAG 
CTTTTGTGTC ACCATTCAAA GACTACAAGG GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT 
GGCCATATAT TTCTTGAAGA AGCAGCCAGA GTGAGTAGTG GTACGGTGGA GCTGCTAAGT 
GAGGGGGAAA GACTGCATCC CAAGGTGATT AAATCATTAA AGAAGGCGCT GGAGCCAGCT 
CTGAGCGATA TCACTATAGA TTGGTACATG CCAGATACTA TGGAGGCATT GCTGACACCT 
AATGAAATTG CTCCACTTTA CCATGGGGAC TCTCTTATTA GTTATTGTGC TTTGTATCAC 
ACAGCCAGCT TCCGTGGCAA GAGGGACATG AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC 
TCAGTCTTTG AGTCCCAGGA AGATTCCCAG GCCCCAGGGA GCACAGACAC AAGCTGTCTA 
AATCCACCCT CAGGTGCTGA TGATATGGGT CGTGCCCTAC AGGAGATTTC CCAGGAAATA 
TCTTTGGAGT TTTCAGCATG CAGTGAGAAG CCAGATATAA GTGAAGATTC TTTCAGTGAC 
ATCAGGAAAC GCATTGTGCG TTCATCATAT GTAAAGGATC AGTATGTCCT CACCCATTGC 
TCTATCAGTA CTGAGCCAAC TCATGTCCAG ACCCACCCCT CCATCAGCAG TGATCCCACT 
AATACCCCTA CTCGTGGCTC TACTAGCAGT GAATCTGTGG GATCCCATGA TGCTAATGGA 
CACTGCATTT CAGCCGTGAC CTCACAACAA GGACAGAAGA CTGTGTCCGA GGTGGCAGGA 
ATTCATCCTA TCCGAGATCT TGCGTCTTCC AGTAAGGCAC CAGGAACCCA GAGCTCTGAA 
GAACTAATCA AAAGGAAGGC ACTGATGCAG GCCACCATGT CTGGTCGCAG TTTCTCGTCT 
CCCCATGGGG AACTGGATAT GCTGCGGCTG CGTTCTGTCT TGGACAAGGT TTCTCAAAGC 
AGGGAGCCAG ATGGGCGCTG CGCACGTGGA GAAAGCTTAA TGGATTCCTG TCATCTCCTT 
ACCCCTTCCC AGTTGGACTG GGACATGTTG GTTGATGCTC AGTATCTGTT TTCTGCTTCT 
CCTGCTGCAG AAGAAAACAG TGTGATATCT AAAGCAAGCA CTGACACTGT ATGGCAGTGC 
AGGGCTGTGA TACGTGGCCT TTTAGGAAAC AAGAGCCACT CTTGGGAGGC CTCAATAAGT 
CTGCTCTCAC TTATGCACTC TAATGAAGAG GAATCGAATG GTCCCTATGT GGTAGATTCT 
GAATTTGATT TGCATCATCT GGCTGCACAG TCTGTCATTA AAGATAATGA GGATACAGCA 
TGGAGAGTGT GTGACATTGA ACATGGGTCT TCTCGTAGAT ATCGCCTGAA AGCCATACAG 
ACCAGCAAAT CATGCTCTCA AATTTCCACA TTTACTAACC ATGTGTCTGT AGATCTTGCT 
ACCCATGATG TTGTTTCGGA GCACATAAAA GTATGTAGAG GTTGGCAAAA TAATAGAACC 
CACAGCACAA ACCTGGCTAA TTTGCATGAA GGAGAAAACA GAAGCTCAGA AAACATAGAT 
GAAGTCCTCA TGGCTTCAGA TGACACTGCA GATTTCCCAT CATCCCCACA GAGCCTAATC 
TCCACCAGTG GTTCGGTAAA ACAGCGTAGT CAGGAAGGTC AAATTCTCCA TGCCTTTGGC 
TCTCAGAAAC CATCAGAATC ATCGCTGAGT TCCAGGATCA GCAATGGAAA AAGAAAAATC 
CGAAGTATTT CTGGAAAACT TTCGCCCCTG AAACCATTAT GCCTAAGTGA GAGGGAGAAG 
GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC TATCTGCCAA TGATTAGCTT GCAGTCCCCA 
GAAGGATACT TCACACTAAA CAGCAATTTC TCCAGTATGG TTAAAATTTC TCTTGAGCGC 
CTGCTGCGTG CCTCACCCTA TAGCTGCCAC CGTGGTAGCT TAAGTCCAGT GTCTGAACCA 
AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA GGAGGAATGG GAACCTACAA GGGGGGTTCC 
CAACCACAGG TTCCCACTGC ACAAGGTCCA TCCTTTATTC CTGCACATGC TACATCATGC 
CCTGAGGCAC TGTGTCAGCA GGCAGACAGT GGACGTGGAT CTGAAACAGA CCCATGTGAA 
ACATCCCCAT GCCCTTCAGA GGTTTGCACT GATATGGACA CTGACCTAGA GGGTTGCTCC 
TGGGCCACTG CCGTGGCATT AGCATGGCTA GAACATTGCT CTGCAGGATT TTTTACTGAA 
TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA TGGTTAAGAG CACAGGTTTT GCCAGATGGG 
CTACAGCTCT CTGCATTAAA AGGAGCTGCC CGCCAGCTTT TCATCTTGCT CCGTCATTGG 
GATGAGAATC TCAACCTGAA TATGCTGTGC TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949902.1
CDS	530..4420
Translation	MPGLYNALSWVQLPLESSSVTAYANGYCMGLNAHLTYLNPGNETVQAVFIYLLDDGEVVTSFEAVTGNQKVQAQVQSQSQMEECCTKFPWDFLPCFCPNGHLILDSNSAHSVFILSVGEVPPSAALSVYLRSSRELKTLPNGAVRVILNSTLTPFAPSPGVPSEEGECLCDDRLCLCPTSCFGGVSGAGGNPWALLSESPPLVLPLPSAGSLAGALAEPATNAQRYQFSFRLQVQGPCLLAGIESTSHALRADASPFAVSASSVWISLAEEHDCNRALEIILHPSEPYIPHLCLERGKKSFSQYKMQIRERGDFIRAVKKSSDPERQVSFVRRRFHKDLHFTPVLMLNFCPDLTALSSLIYDLPTITREVIFLVYHSGELSHNVRDALLLAVRSLPARTLLNLGSGTCDSITLFPTSRQCSNESLEAAYDHIKNGQLSCSSSNLVTVLGHILSLPVHRGYPRQLFLFTDTSPRCPERAIELLCHHSKTTRVFGFGLGVKAGHIFLEEAARVSSGTVELLSEGERLHPKVIKSLKKALEPALSDITIDWYMPDTMEALLTPNEIAPLYHGDSLISYCALYHTASFRGKRDMRKSARCGSFSSVFESQEDSQAPGSTDTSCLNPPSGADDMGRALQEISQEISLEFSACSEKPDISEDSFSDIRKRIVRSSYVKDQYVLTHCSISTEPTHVQTHPSISSDPTNTPTRGSTSSESVGSHDANGHCISAVTSQQGQKTVSEVAGIHPIRDLASSSKAPGTQSSEELIKRKALMQATMSGRSFSSPHGELDMLRLRSVLDKVSQSREPDGRCARGESLMDSCHLLTPSQLDWDMLVDAQYLFSASPAAEENSVISKASTDTVWQCRAVIRGLLGNKSHSWEASISLLSLMHSNEEESNGPYVVDSEFDLHHLAAQSVIKDNEDTAWRVCDIEHGSSRRYRLKAIQTSKSCSQISTFTNHVSVDLATHDVVSEHIKVCRGWQNNRTHSTNLANLHEGENRSSENIDEVLMASDDTADFPSSPQSLISTSGSVKQRSQEGQILHAFGSQKPSESSLSSRISNGKRKIRSISGKLSPLKPLCLSEREKERSEESGFQDYLPMISLQSPEGYFTLNSNFSSMVKISLERLLRASPYSCHRGSLSPVSEPSLLEQGHFLKGGMGTYKGGSQPQVPTAQGPSFIPAHATSCPEALCQQADSGRGSETDPCETSPCPSEVCTDMDTDLEGCSWATAVALAWLEHCSAGFFTEWEVLAAKAEVWLRAQVLPDGLQLSALKGAARQLFILLRHWDENLNLNMLCYKPGNV

Target ORF information:

RefSeq Version	XM_018094413.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094413.2

ORF Insert Sequence:

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGGT TGTGCTTATG TCCAACCAGC 
TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA AATCCATGGG CGTTGCTCAG TGAATCCCCT 
CCTCTGGTCC TGCCTTTACC CTCTGCAGGT TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT 
ACCAATGCAC AGCGTTATCA ATTCAGCTTT CGTCTCCAAG TACAAGGACC TTGTCTTCTG 
GCAGGAATAG AAAGTACTTC TCATGCTCTC CGTGCTGATG CTAGTCCATT TGCTGTGTCA 
GCCTCTTCTG TTTGGATCAG TTTGGCAGAA GAACATGACT GTAACCGAGC TTTGGAAATA 
ATCCTGCATC CTAGTGAACC ATACATTCCT CACTTATGTT TGGAGAGAGG CAAAAAAAGT 
TTCTCTCAAT ACAAAATGCA GATAAGAGAG AGAGGAGACT TCATAAGGGC AGTTAAGAAA 
TCTAGTGACC CAGAAAGACA GGTCAGCTTT GTAAGGAGAC GCTTCCACAA AGATCTCCAT 
TTTACCCCAG TCTTGATGTT GAACTTCTGC CCTGACCTTA CTGCCCTCAG CTCATTAATC 
TATGACCTCC CTACTATAAC CAGAGAAGTC ATTTTCCTTG TTTATCACTC AGGGGAGCTT 
TCGCATAATG TCCGGGATGC TCTCTTGCTG GCTGTTAGGA GTTTACCTGC ACGTACTTTG 
CTGAATCTGG GAAGTGGCAC ATGTGACAGC ATAACCCTGT TTCCGACCAG CAGGCAGTGT 
AGCAATGAGT CATTGGAGGC AGCTTATGAT CATATTAAGA ATGGACAACT CAGTTGCAGT 
TCAAGTAACT TGGTGACTGT TCTGGGCCAT ATCCTGTCTC TTCCAGTGCA TCGTGGATAC 
CCACGGCAGC TCTTTCTCTT CACTGACACA TCTCCAAGGT GTCCTGAAAG AGCCATTGAG 
CTTTTGTGTC ACCATTCAAA GACTACAAGG GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT 
GGCCATATAT TTCTTGAAGA AGCAGCCAGA GTGAGTAGTG GTACGGTGGA GCTGCTAAGT 
GAGGGGGAAA GACTGCATCC CAAGGTGATT AAATCATTAA AGAAGGCGCT GGAGCCAGCT 
CTGAGCGATA TCACTATAGA TTGGTACATG CCAGATACTA TGGAGGCATT GCTGACACCT 
AATGAAATTG CTCCACTTTA CCATGGGGAC TCTCTTATTA GTTATTGTGC TTTGTATCAC 
ACAGCCAGCT TCCGTGGCAA GAGGGACATG AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC 
TCAGTCTTTG AGTCCCAGGA AGATTCCCAG GCCCCAGGGA GCACAGACAC AAGCTGTCTA 
AATCCACCCT CAGGTGCTGA TGATATGGGT CGTGCCCTAC AGGAGATTTC CCAGGAAATA 
TCTTTGGAGT TTTCAGCATG CAGTGAGAAG CCAGATATAA GTGAAGATTC TTTCAGTGAC 
ATCAGGAAAC GCATTGTGCG TTCATCATAT GTAAAGGATC AGTATGTCCT CACCCATTGC 
TCTATCAGTA CTGAGCCAAC TCATGTCCAG ACCCACCCCT CCATCAGCAG TGATCCCACT 
AATACCCCTA CTCGTGGCTC TACTAGCAGT GAATCTGTGG GATCCCATGA TGCTAATGGA 
CACTGCATTT CAGCCGTGAC CTCACAACAA GGACAGAAGA CTGTGTCCGA GGTGGCAGGA 
ATTCATCCTA TCCGAGATCT TGCGTCTTCC AGTAAGGCAC CAGGAACCCA GAGCTCTGAA 
GAACTAATCA AAAGGAAGGC ACTGATGCAG GCCACCATGT CTGGTCGCAG TTTCTCGTCT 
CCCCATGGGG AACTGGATAT GCTGCGGCTG CGTTCTGTCT TGGACAAGGT TTCTCAAAGC 
AGGGAGCCAG ATGGGCGCTG CGCACGTGGA GAAAGCTTAA TGGATTCCTG TCATCTCCTT 
ACCCCTTCCC AGTTGGACTG GGACATGTTG GTTGATGCTC AGTATCTGTT TTCTGCTTCT 
CCTGCTGCAG AAGAAAACAG TGTGATATCT AAAGCAAGCA CTGACACTGT ATGGCAGTGC 
AGGGCTGTGA TACGTGGCCT TTTAGGAAAC AAGAGCCACT CTTGGGAGGC CTCAATAAGT 
CTGCTCTCAC TTATGCACTC TAATGAAGAG GAATCGAATG GTCCCTATGT GGTAGATTCT 
GAATTTGATT TGCATCATCT GGCTGCACAG TCTGTCATTA AAGATAATGA GGATACAGCA 
TGGAGAGTGT GTGACATTGA ACATGGGTCT TCTCGTAGAT ATCGCCTGAA AGCCATACAG 
ACCAGCAAAT CATGCTCTCA AATTTCCACA TTTACTAACC ATGTGTCTGT AGATCTTGCT 
ACCCATGATG TTGTTTCGGA GCACATAAAA GTATGTAGAG GTTGGCAAAA TAATAGAACC 
CACAGCACAA ACCTGGCTAA TTTGCATGAA GGAGAAAACA GAAGCTCAGA AAACATAGAT 
GAAGTCCTCA TGGCTTCAGA TGACACTGCA GATTTCCCAT CATCCCCACA GAGCCTAATC 
TCCACCAGTG GTTCGGTAAA ACAGCGTAGT CAGGAAGGTC AAATTCTCCA TGCCTTTGGC 
TCTCAGAAAC CATCAGAATC ATCGCTGAGT TCCAGGATCA GCAATGGAAA AAGAAAAATC 
CGAAGTATTT CTGGAAAACT TTCGCCCCTG AAACCATTAT GCCTAAGTGA GAGGGAGAAG 
GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC TATCTGCCAA TGATTAGCTT GCAGTCCCCA 
GAAGGATACT TCACACTAAA CAGCAATTTC TCCAGTATGG TTAAAATTTC TCTTGAGCGC 
CTGCTGCGTG CCTCACCCTA TAGCTGCCAC CGTGGTAGCT TAAGTCCAGT GTCTGAACCA 
AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA GGAGGAATGG GAACCTACAA GGGGGGTTCC 
CAACCACAGG TTCCCACTGC ACAAGGTCCA TCCTTTATTC CTGCACATGC TACATCATGC 
CCTGAGGCAC TGTGTCAGCA GGCAGACAGT GGACGTGGAT CTGAAACAGA CCCATGTGAA 
ACATCCCCAT GCCCTTCAGA GGTTTGCACT GATATGGACA CTGACCTAGA GGGTTGCTCC 
TGGGCCACTG CCGTGGCATT AGCATGGCTA GAACATTGCT CTGCAGGATT TTTTACTGAA 
TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA TGGTTAAGAG CACAGGTTTT GCCAGATGGG 
CTACAGCTCT CTGCATTAAA AGGAGCTGCC CGCCAGCTTT TCATCTTGCT CCGTCATTGG 
GATGAGAATC TCAACCTGAA TATGCTGTGC TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa5b2 ( XM_012963368.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818822.1 Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa34064
Clone ID Related Accession (Same CDS sequence)	XM_018094413.2 , XM_012963368.2 , XM_012963369.2 , XM_012963368.3 , XM_012963369.3 , XM_018094413.1
Accession Version	XM_012963368.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor A domain-containing protein 5B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963368.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGGT TGTGCTTATG TCCAACCAGC 
TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA AATCCATGGG CGTTGCTCAG TGAATCCCCT 
CCTCTGGTCC TGCCTTTACC CTCTGCAGGT TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT 
ACCAATGCAC AGCGTTATCA ATTCAGCTTT CGTCTCCAAG TACAAGGACC TTGTCTTCTG 
GCAGGAATAG AAAGTACTTC TCATGCTCTC CGTGCTGATG CTAGTCCATT TGCTGTGTCA 
GCCTCTTCTG TTTGGATCAG TTTGGCAGAA GAACATGACT GTAACCGAGC TTTGGAAATA 
ATCCTGCATC CTAGTGAACC ATACATTCCT CACTTATGTT TGGAGAGAGG CAAAAAAAGT 
TTCTCTCAAT ACAAAATGCA GATAAGAGAG AGAGGAGACT TCATAAGGGC AGTTAAGAAA 
TCTAGTGACC CAGAAAGACA GGTCAGCTTT GTAAGGAGAC GCTTCCACAA AGATCTCCAT 
TTTACCCCAG TCTTGATGTT GAACTTCTGC CCTGACCTTA CTGCCCTCAG CTCATTAATC 
TATGACCTCC CTACTATAAC CAGAGAAGTC ATTTTCCTTG TTTATCACTC AGGGGAGCTT 
TCGCATAATG TCCGGGATGC TCTCTTGCTG GCTGTTAGGA GTTTACCTGC ACGTACTTTG 
CTGAATCTGG GAAGTGGCAC ATGTGACAGC ATAACCCTGT TTCCGACCAG CAGGCAGTGT 
AGCAATGAGT CATTGGAGGC AGCTTATGAT CATATTAAGA ATGGACAACT CAGTTGCAGT 
TCAAGTAACT TGGTGACTGT TCTGGGCCAT ATCCTGTCTC TTCCAGTGCA TCGTGGATAC 
CCACGGCAGC TCTTTCTCTT CACTGACACA TCTCCAAGGT GTCCTGAAAG AGCCATTGAG 
CTTTTGTGTC ACCATTCAAA GACTACAAGG GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT 
GGCCATATAT TTCTTGAAGA AGCAGCCAGA GTGAGTAGTG GTACGGTGGA GCTGCTAAGT 
GAGGGGGAAA GACTGCATCC CAAGGTGATT AAATCATTAA AGAAGGCGCT GGAGCCAGCT 
CTGAGCGATA TCACTATAGA TTGGTACATG CCAGATACTA TGGAGGCATT GCTGACACCT 
AATGAAATTG CTCCACTTTA CCATGGGGAC TCTCTTATTA GTTATTGTGC TTTGTATCAC 
ACAGCCAGCT TCCGTGGCAA GAGGGACATG AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC 
TCAGTCTTTG AGTCCCAGGA AGATTCCCAG GCCCCAGGGA GCACAGACAC AAGCTGTCTA 
AATCCACCCT CAGGTGCTGA TGATATGGGT CGTGCCCTAC AGGAGATTTC CCAGGAAATA 
TCTTTGGAGT TTTCAGCATG CAGTGAGAAG CCAGATATAA GTGAAGATTC TTTCAGTGAC 
ATCAGGAAAC GCATTGTGCG TTCATCATAT GTAAAGGATC AGTATGTCCT CACCCATTGC 
TCTATCAGTA CTGAGCCAAC TCATGTCCAG ACCCACCCCT CCATCAGCAG TGATCCCACT 
AATACCCCTA CTCGTGGCTC TACTAGCAGT GAATCTGTGG GATCCCATGA TGCTAATGGA 
CACTGCATTT CAGCCGTGAC CTCACAACAA GGACAGAAGA CTGTGTCCGA GGTGGCAGGA 
ATTCATCCTA TCCGAGATCT TGCGTCTTCC AGTAAGGCAC CAGGAACCCA GAGCTCTGAA 
GAACTAATCA AAAGGAAGGC ACTGATGCAG GCCACCATGT CTGGTCGCAG TTTCTCGTCT 
CCCCATGGGG AACTGGATAT GCTGCGGCTG CGTTCTGTCT TGGACAAGGT TTCTCAAAGC 
AGGGAGCCAG ATGGGCGCTG CGCACGTGGA GAAAGCTTAA TGGATTCCTG TCATCTCCTT 
ACCCCTTCCC AGTTGGACTG GGACATGTTG GTTGATGCTC AGTATCTGTT TTCTGCTTCT 
CCTGCTGCAG AAGAAAACAG TGTGATATCT AAAGCAAGCA CTGACACTGT ATGGCAGTGC 
AGGGCTGTGA TACGTGGCCT TTTAGGAAAC AAGAGCCACT CTTGGGAGGC CTCAATAAGT 
CTGCTCTCAC TTATGCACTC TAATGAAGAG GAATCGAATG GTCCCTATGT GGTAGATTCT 
GAATTTGATT TGCATCATCT GGCTGCACAG TCTGTCATTA AAGATAATGA GGATACAGCA 
TGGAGAGTGT GTGACATTGA ACATGGGTCT TCTCGTAGAT ATCGCCTGAA AGCCATACAG 
ACCAGCAAAT CATGCTCTCA AATTTCCACA TTTACTAACC ATGTGTCTGT AGATCTTGCT 
ACCCATGATG TTGTTTCGGA GCACATAAAA GTATGTAGAG GTTGGCAAAA TAATAGAACC 
CACAGCACAA ACCTGGCTAA TTTGCATGAA GGAGAAAACA GAAGCTCAGA AAACATAGAT 
GAAGTCCTCA TGGCTTCAGA TGACACTGCA GATTTCCCAT CATCCCCACA GAGCCTAATC 
TCCACCAGTG GTTCGGTAAA ACAGCGTAGT CAGGAAGGTC AAATTCTCCA TGCCTTTGGC 
TCTCAGAAAC CATCAGAATC ATCGCTGAGT TCCAGGATCA GCAATGGAAA AAGAAAAATC 
CGAAGTATTT CTGGAAAACT TTCGCCCCTG AAACCATTAT GCCTAAGTGA GAGGGAGAAG 
GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC TATCTGCCAA TGATTAGCTT GCAGTCCCCA 
GAAGGATACT TCACACTAAA CAGCAATTTC TCCAGTATGG TTAAAATTTC TCTTGAGCGC 
CTGCTGCGTG CCTCACCCTA TAGCTGCCAC CGTGGTAGCT TAAGTCCAGT GTCTGAACCA 
AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA GGAGGAATGG GAACCTACAA GGGGGGTTCC 
CAACCACAGG TTCCCACTGC ACAAGGTCCA TCCTTTATTC CTGCACATGC TACATCATGC 
CCTGAGGCAC TGTGTCAGCA GGCAGACAGT GGACGTGGAT CTGAAACAGA CCCATGTGAA 
ACATCCCCAT GCCCTTCAGA GGTTTGCACT GATATGGACA CTGACCTAGA GGGTTGCTCC 
TGGGCCACTG CCGTGGCATT AGCATGGCTA GAACATTGCT CTGCAGGATT TTTTACTGAA 
TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA TGGTTAAGAG CACAGGTTTT GCCAGATGGG 
CTACAGCTCT CTGCATTAAA AGGAGCTGCC CGCCAGCTTT TCATCTTGCT CCGTCATTGG 
GATGAGAATC TCAACCTGAA TATGCTGTGC TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818822.1
CDS	602..4492
Translation	MPGLYNALSWVQLPLESSSVTAYANGYCMGLNAHLTYLNPGNETVQAVFIYLLDDGEVVTSFEAVTGNQKVQAQVQSQSQMEECCTKFPWDFLPCFCPNGHLILDSNSAHSVFILSVGEVPPSAALSVYLRSSRELKTLPNGAVRVILNSTLTPFAPSPGVPSEEGECLCDDRLCLCPTSCFGGVSGAGGNPWALLSESPPLVLPLPSAGSLAGALAEPATNAQRYQFSFRLQVQGPCLLAGIESTSHALRADASPFAVSASSVWISLAEEHDCNRALEIILHPSEPYIPHLCLERGKKSFSQYKMQIRERGDFIRAVKKSSDPERQVSFVRRRFHKDLHFTPVLMLNFCPDLTALSSLIYDLPTITREVIFLVYHSGELSHNVRDALLLAVRSLPARTLLNLGSGTCDSITLFPTSRQCSNESLEAAYDHIKNGQLSCSSSNLVTVLGHILSLPVHRGYPRQLFLFTDTSPRCPERAIELLCHHSKTTRVFGFGLGVKAGHIFLEEAARVSSGTVELLSEGERLHPKVIKSLKKALEPALSDITIDWYMPDTMEALLTPNEIAPLYHGDSLISYCALYHTASFRGKRDMRKSARCGSFSSVFESQEDSQAPGSTDTSCLNPPSGADDMGRALQEISQEISLEFSACSEKPDISEDSFSDIRKRIVRSSYVKDQYVLTHCSISTEPTHVQTHPSISSDPTNTPTRGSTSSESVGSHDANGHCISAVTSQQGQKTVSEVAGIHPIRDLASSSKAPGTQSSEELIKRKALMQATMSGRSFSSPHGELDMLRLRSVLDKVSQSREPDGRCARGESLMDSCHLLTPSQLDWDMLVDAQYLFSASPAAEENSVISKASTDTVWQCRAVIRGLLGNKSHSWEASISLLSLMHSNEEESNGPYVVDSEFDLHHLAAQSVIKDNEDTAWRVCDIEHGSSRRYRLKAIQTSKSCSQISTFTNHVSVDLATHDVVSEHIKVCRGWQNNRTHSTNLANLHEGENRSSENIDEVLMASDDTADFPSSPQSLISTSGSVKQRSQEGQILHAFGSQKPSESSLSSRISNGKRKIRSISGKLSPLKPLCLSEREKERSEESGFQDYLPMISLQSPEGYFTLNSNFSSMVKISLERLLRASPYSCHRGSLSPVSEPSLLEQGHFLKGGMGTYKGGSQPQVPTAQGPSFIPAHATSCPEALCQQADSGRGSETDPCETSPCPSEVCTDMDTDLEGCSWATAVALAWLEHCSAGFFTEWEVLAAKAEVWLRAQVLPDGLQLSALKGAARQLFILLRHWDENLNLNMLCYKPGNV

Target ORF information:

RefSeq Version	XM_012963368.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963368.3

ORF Insert Sequence:

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGGT TGTGCTTATG TCCAACCAGC 
TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA AATCCATGGG CGTTGCTCAG TGAATCCCCT 
CCTCTGGTCC TGCCTTTACC CTCTGCAGGT TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT 
ACCAATGCAC AGCGTTATCA ATTCAGCTTT CGTCTCCAAG TACAAGGACC TTGTCTTCTG 
GCAGGAATAG AAAGTACTTC TCATGCTCTC CGTGCTGATG CTAGTCCATT TGCTGTGTCA 
GCCTCTTCTG TTTGGATCAG TTTGGCAGAA GAACATGACT GTAACCGAGC TTTGGAAATA 
ATCCTGCATC CTAGTGAACC ATACATTCCT CACTTATGTT TGGAGAGAGG CAAAAAAAGT 
TTCTCTCAAT ACAAAATGCA GATAAGAGAG AGAGGAGACT TCATAAGGGC AGTTAAGAAA 
TCTAGTGACC CAGAAAGACA GGTCAGCTTT GTAAGGAGAC GCTTCCACAA AGATCTCCAT 
TTTACCCCAG TCTTGATGTT GAACTTCTGC CCTGACCTTA CTGCCCTCAG CTCATTAATC 
TATGACCTCC CTACTATAAC CAGAGAAGTC ATTTTCCTTG TTTATCACTC AGGGGAGCTT 
TCGCATAATG TCCGGGATGC TCTCTTGCTG GCTGTTAGGA GTTTACCTGC ACGTACTTTG 
CTGAATCTGG GAAGTGGCAC ATGTGACAGC ATAACCCTGT TTCCGACCAG CAGGCAGTGT 
AGCAATGAGT CATTGGAGGC AGCTTATGAT CATATTAAGA ATGGACAACT CAGTTGCAGT 
TCAAGTAACT TGGTGACTGT TCTGGGCCAT ATCCTGTCTC TTCCAGTGCA TCGTGGATAC 
CCACGGCAGC TCTTTCTCTT CACTGACACA TCTCCAAGGT GTCCTGAAAG AGCCATTGAG 
CTTTTGTGTC ACCATTCAAA GACTACAAGG GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT 
GGCCATATAT TTCTTGAAGA AGCAGCCAGA GTGAGTAGTG GTACGGTGGA GCTGCTAAGT 
GAGGGGGAAA GACTGCATCC CAAGGTGATT AAATCATTAA AGAAGGCGCT GGAGCCAGCT 
CTGAGCGATA TCACTATAGA TTGGTACATG CCAGATACTA TGGAGGCATT GCTGACACCT 
AATGAAATTG CTCCACTTTA CCATGGGGAC TCTCTTATTA GTTATTGTGC TTTGTATCAC 
ACAGCCAGCT TCCGTGGCAA GAGGGACATG AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC 
TCAGTCTTTG AGTCCCAGGA AGATTCCCAG GCCCCAGGGA GCACAGACAC AAGCTGTCTA 
AATCCACCCT CAGGTGCTGA TGATATGGGT CGTGCCCTAC AGGAGATTTC CCAGGAAATA 
TCTTTGGAGT TTTCAGCATG CAGTGAGAAG CCAGATATAA GTGAAGATTC TTTCAGTGAC 
ATCAGGAAAC GCATTGTGCG TTCATCATAT GTAAAGGATC AGTATGTCCT CACCCATTGC 
TCTATCAGTA CTGAGCCAAC TCATGTCCAG ACCCACCCCT CCATCAGCAG TGATCCCACT 
AATACCCCTA CTCGTGGCTC TACTAGCAGT GAATCTGTGG GATCCCATGA TGCTAATGGA 
CACTGCATTT CAGCCGTGAC CTCACAACAA GGACAGAAGA CTGTGTCCGA GGTGGCAGGA 
ATTCATCCTA TCCGAGATCT TGCGTCTTCC AGTAAGGCAC CAGGAACCCA GAGCTCTGAA 
GAACTAATCA AAAGGAAGGC ACTGATGCAG GCCACCATGT CTGGTCGCAG TTTCTCGTCT 
CCCCATGGGG AACTGGATAT GCTGCGGCTG CGTTCTGTCT TGGACAAGGT TTCTCAAAGC 
AGGGAGCCAG ATGGGCGCTG CGCACGTGGA GAAAGCTTAA TGGATTCCTG TCATCTCCTT 
ACCCCTTCCC AGTTGGACTG GGACATGTTG GTTGATGCTC AGTATCTGTT TTCTGCTTCT 
CCTGCTGCAG AAGAAAACAG TGTGATATCT AAAGCAAGCA CTGACACTGT ATGGCAGTGC 
AGGGCTGTGA TACGTGGCCT TTTAGGAAAC AAGAGCCACT CTTGGGAGGC CTCAATAAGT 
CTGCTCTCAC TTATGCACTC TAATGAAGAG GAATCGAATG GTCCCTATGT GGTAGATTCT 
GAATTTGATT TGCATCATCT GGCTGCACAG TCTGTCATTA AAGATAATGA GGATACAGCA 
TGGAGAGTGT GTGACATTGA ACATGGGTCT TCTCGTAGAT ATCGCCTGAA AGCCATACAG 
ACCAGCAAAT CATGCTCTCA AATTTCCACA TTTACTAACC ATGTGTCTGT AGATCTTGCT 
ACCCATGATG TTGTTTCGGA GCACATAAAA GTATGTAGAG GTTGGCAAAA TAATAGAACC 
CACAGCACAA ACCTGGCTAA TTTGCATGAA GGAGAAAACA GAAGCTCAGA AAACATAGAT 
GAAGTCCTCA TGGCTTCAGA TGACACTGCA GATTTCCCAT CATCCCCACA GAGCCTAATC 
TCCACCAGTG GTTCGGTAAA ACAGCGTAGT CAGGAAGGTC AAATTCTCCA TGCCTTTGGC 
TCTCAGAAAC CATCAGAATC ATCGCTGAGT TCCAGGATCA GCAATGGAAA AAGAAAAATC 
CGAAGTATTT CTGGAAAACT TTCGCCCCTG AAACCATTAT GCCTAAGTGA GAGGGAGAAG 
GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC TATCTGCCAA TGATTAGCTT GCAGTCCCCA 
GAAGGATACT TCACACTAAA CAGCAATTTC TCCAGTATGG TTAAAATTTC TCTTGAGCGC 
CTGCTGCGTG CCTCACCCTA TAGCTGCCAC CGTGGTAGCT TAAGTCCAGT GTCTGAACCA 
AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA GGAGGAATGG GAACCTACAA GGGGGGTTCC 
CAACCACAGG TTCCCACTGC ACAAGGTCCA TCCTTTATTC CTGCACATGC TACATCATGC 
CCTGAGGCAC TGTGTCAGCA GGCAGACAGT GGACGTGGAT CTGAAACAGA CCCATGTGAA 
ACATCCCCAT GCCCTTCAGA GGTTTGCACT GATATGGACA CTGACCTAGA GGGTTGCTCC 
TGGGCCACTG CCGTGGCATT AGCATGGCTA GAACATTGCT CTGCAGGATT TTTTACTGAA 
TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA TGGTTAAGAG CACAGGTTTT GCCAGATGGG 
CTACAGCTCT CTGCATTAAA AGGAGCTGCC CGCCAGCTTT TCATCTTGCT CCGTCATTGG 
GATGAGAATC TCAACCTGAA TATGCTGTGC TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa5b2 ( XM_012963369.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818823.1 Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X2, mRNA.

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CloneID	OXa34064
Clone ID Related Accession (Same CDS sequence)	XM_018094413.2 , XM_012963368.2 , XM_012963369.2 , XM_012963368.3 , XM_012963369.3 , XM_018094413.1
Accession Version	XM_012963369.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor A domain-containing protein 5B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963369.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGGT TGTGCTTATG TCCAACCAGC 
TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA AATCCATGGG CGTTGCTCAG TGAATCCCCT 
CCTCTGGTCC TGCCTTTACC CTCTGCAGGT TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT 
ACCAATGCAC AGCGTTATCA ATTCAGCTTT CGTCTCCAAG TACAAGGACC TTGTCTTCTG 
GCAGGAATAG AAAGTACTTC TCATGCTCTC CGTGCTGATG CTAGTCCATT TGCTGTGTCA 
GCCTCTTCTG TTTGGATCAG TTTGGCAGAA GAACATGACT GTAACCGAGC TTTGGAAATA 
ATCCTGCATC CTAGTGAACC ATACATTCCT CACTTATGTT TGGAGAGAGG CAAAAAAAGT 
TTCTCTCAAT ACAAAATGCA GATAAGAGAG AGAGGAGACT TCATAAGGGC AGTTAAGAAA 
TCTAGTGACC CAGAAAGACA GGTCAGCTTT GTAAGGAGAC GCTTCCACAA AGATCTCCAT 
TTTACCCCAG TCTTGATGTT GAACTTCTGC CCTGACCTTA CTGCCCTCAG CTCATTAATC 
TATGACCTCC CTACTATAAC CAGAGAAGTC ATTTTCCTTG TTTATCACTC AGGGGAGCTT 
TCGCATAATG TCCGGGATGC TCTCTTGCTG GCTGTTAGGA GTTTACCTGC ACGTACTTTG 
CTGAATCTGG GAAGTGGCAC ATGTGACAGC ATAACCCTGT TTCCGACCAG CAGGCAGTGT 
AGCAATGAGT CATTGGAGGC AGCTTATGAT CATATTAAGA ATGGACAACT CAGTTGCAGT 
TCAAGTAACT TGGTGACTGT TCTGGGCCAT ATCCTGTCTC TTCCAGTGCA TCGTGGATAC 
CCACGGCAGC TCTTTCTCTT CACTGACACA TCTCCAAGGT GTCCTGAAAG AGCCATTGAG 
CTTTTGTGTC ACCATTCAAA GACTACAAGG GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT 
GGCCATATAT TTCTTGAAGA AGCAGCCAGA GTGAGTAGTG GTACGGTGGA GCTGCTAAGT 
GAGGGGGAAA GACTGCATCC CAAGGTGATT AAATCATTAA AGAAGGCGCT GGAGCCAGCT 
CTGAGCGATA TCACTATAGA TTGGTACATG CCAGATACTA TGGAGGCATT GCTGACACCT 
AATGAAATTG CTCCACTTTA CCATGGGGAC TCTCTTATTA GTTATTGTGC TTTGTATCAC 
ACAGCCAGCT TCCGTGGCAA GAGGGACATG AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC 
TCAGTCTTTG AGTCCCAGGA AGATTCCCAG GCCCCAGGGA GCACAGACAC AAGCTGTCTA 
AATCCACCCT CAGGTGCTGA TGATATGGGT CGTGCCCTAC AGGAGATTTC CCAGGAAATA 
TCTTTGGAGT TTTCAGCATG CAGTGAGAAG CCAGATATAA GTGAAGATTC TTTCAGTGAC 
ATCAGGAAAC GCATTGTGCG TTCATCATAT GTAAAGGATC AGTATGTCCT CACCCATTGC 
TCTATCAGTA CTGAGCCAAC TCATGTCCAG ACCCACCCCT CCATCAGCAG TGATCCCACT 
AATACCCCTA CTCGTGGCTC TACTAGCAGT GAATCTGTGG GATCCCATGA TGCTAATGGA 
CACTGCATTT CAGCCGTGAC CTCACAACAA GGACAGAAGA CTGTGTCCGA GGTGGCAGGA 
ATTCATCCTA TCCGAGATCT TGCGTCTTCC AGTAAGGCAC CAGGAACCCA GAGCTCTGAA 
GAACTAATCA AAAGGAAGGC ACTGATGCAG GCCACCATGT CTGGTCGCAG TTTCTCGTCT 
CCCCATGGGG AACTGGATAT GCTGCGGCTG CGTTCTGTCT TGGACAAGGT TTCTCAAAGC 
AGGGAGCCAG ATGGGCGCTG CGCACGTGGA GAAAGCTTAA TGGATTCCTG TCATCTCCTT 
ACCCCTTCCC AGTTGGACTG GGACATGTTG GTTGATGCTC AGTATCTGTT TTCTGCTTCT 
CCTGCTGCAG AAGAAAACAG TGTGATATCT AAAGCAAGCA CTGACACTGT ATGGCAGTGC 
AGGGCTGTGA TACGTGGCCT TTTAGGAAAC AAGAGCCACT CTTGGGAGGC CTCAATAAGT 
CTGCTCTCAC TTATGCACTC TAATGAAGAG GAATCGAATG GTCCCTATGT GGTAGATTCT 
GAATTTGATT TGCATCATCT GGCTGCACAG TCTGTCATTA AAGATAATGA GGATACAGCA 
TGGAGAGTGT GTGACATTGA ACATGGGTCT TCTCGTAGAT ATCGCCTGAA AGCCATACAG 
ACCAGCAAAT CATGCTCTCA AATTTCCACA TTTACTAACC ATGTGTCTGT AGATCTTGCT 
ACCCATGATG TTGTTTCGGA GCACATAAAA GTATGTAGAG GTTGGCAAAA TAATAGAACC 
CACAGCACAA ACCTGGCTAA TTTGCATGAA GGAGAAAACA GAAGCTCAGA AAACATAGAT 
GAAGTCCTCA TGGCTTCAGA TGACACTGCA GATTTCCCAT CATCCCCACA GAGCCTAATC 
TCCACCAGTG GTTCGGTAAA ACAGCGTAGT CAGGAAGGTC AAATTCTCCA TGCCTTTGGC 
TCTCAGAAAC CATCAGAATC ATCGCTGAGT TCCAGGATCA GCAATGGAAA AAGAAAAATC 
CGAAGTATTT CTGGAAAACT TTCGCCCCTG AAACCATTAT GCCTAAGTGA GAGGGAGAAG 
GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC TATCTGCCAA TGATTAGCTT GCAGTCCCCA 
GAAGGATACT TCACACTAAA CAGCAATTTC TCCAGTATGG TTAAAATTTC TCTTGAGCGC 
CTGCTGCGTG CCTCACCCTA TAGCTGCCAC CGTGGTAGCT TAAGTCCAGT GTCTGAACCA 
AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA GGAGGAATGG GAACCTACAA GGGGGGTTCC 
CAACCACAGG TTCCCACTGC ACAAGGTCCA TCCTTTATTC CTGCACATGC TACATCATGC 
CCTGAGGCAC TGTGTCAGCA GGCAGACAGT GGACGTGGAT CTGAAACAGA CCCATGTGAA 
ACATCCCCAT GCCCTTCAGA GGTTTGCACT GATATGGACA CTGACCTAGA GGGTTGCTCC 
TGGGCCACTG CCGTGGCATT AGCATGGCTA GAACATTGCT CTGCAGGATT TTTTACTGAA 
TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA TGGTTAAGAG CACAGGTTTT GCCAGATGGG 
CTACAGCTCT CTGCATTAAA AGGAGCTGCC CGCCAGCTTT TCATCTTGCT CCGTCATTGG 
GATGAGAATC TCAACCTGAA TATGCTGTGC TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818823.1
CDS	578..4468
Translation	MPGLYNALSWVQLPLESSSVTAYANGYCMGLNAHLTYLNPGNETVQAVFIYLLDDGEVVTSFEAVTGNQKVQAQVQSQSQMEECCTKFPWDFLPCFCPNGHLILDSNSAHSVFILSVGEVPPSAALSVYLRSSRELKTLPNGAVRVILNSTLTPFAPSPGVPSEEGECLCDDRLCLCPTSCFGGVSGAGGNPWALLSESPPLVLPLPSAGSLAGALAEPATNAQRYQFSFRLQVQGPCLLAGIESTSHALRADASPFAVSASSVWISLAEEHDCNRALEIILHPSEPYIPHLCLERGKKSFSQYKMQIRERGDFIRAVKKSSDPERQVSFVRRRFHKDLHFTPVLMLNFCPDLTALSSLIYDLPTITREVIFLVYHSGELSHNVRDALLLAVRSLPARTLLNLGSGTCDSITLFPTSRQCSNESLEAAYDHIKNGQLSCSSSNLVTVLGHILSLPVHRGYPRQLFLFTDTSPRCPERAIELLCHHSKTTRVFGFGLGVKAGHIFLEEAARVSSGTVELLSEGERLHPKVIKSLKKALEPALSDITIDWYMPDTMEALLTPNEIAPLYHGDSLISYCALYHTASFRGKRDMRKSARCGSFSSVFESQEDSQAPGSTDTSCLNPPSGADDMGRALQEISQEISLEFSACSEKPDISEDSFSDIRKRIVRSSYVKDQYVLTHCSISTEPTHVQTHPSISSDPTNTPTRGSTSSESVGSHDANGHCISAVTSQQGQKTVSEVAGIHPIRDLASSSKAPGTQSSEELIKRKALMQATMSGRSFSSPHGELDMLRLRSVLDKVSQSREPDGRCARGESLMDSCHLLTPSQLDWDMLVDAQYLFSASPAAEENSVISKASTDTVWQCRAVIRGLLGNKSHSWEASISLLSLMHSNEEESNGPYVVDSEFDLHHLAAQSVIKDNEDTAWRVCDIEHGSSRRYRLKAIQTSKSCSQISTFTNHVSVDLATHDVVSEHIKVCRGWQNNRTHSTNLANLHEGENRSSENIDEVLMASDDTADFPSSPQSLISTSGSVKQRSQEGQILHAFGSQKPSESSLSSRISNGKRKIRSISGKLSPLKPLCLSEREKERSEESGFQDYLPMISLQSPEGYFTLNSNFSSMVKISLERLLRASPYSCHRGSLSPVSEPSLLEQGHFLKGGMGTYKGGSQPQVPTAQGPSFIPAHATSCPEALCQQADSGRGSETDPCETSPCPSEVCTDMDTDLEGCSWATAVALAWLEHCSAGFFTEWEVLAAKAEVWLRAQVLPDGLQLSALKGAARQLFILLRHWDENLNLNMLCYKPGNV

Target ORF information:

RefSeq Version	XM_012963369.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963369.3

ORF Insert Sequence:

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGGT TGTGCTTATG TCCAACCAGC 
TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA AATCCATGGG CGTTGCTCAG TGAATCCCCT 
CCTCTGGTCC TGCCTTTACC CTCTGCAGGT TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT 
ACCAATGCAC AGCGTTATCA ATTCAGCTTT CGTCTCCAAG TACAAGGACC TTGTCTTCTG 
GCAGGAATAG AAAGTACTTC TCATGCTCTC CGTGCTGATG CTAGTCCATT TGCTGTGTCA 
GCCTCTTCTG TTTGGATCAG TTTGGCAGAA GAACATGACT GTAACCGAGC TTTGGAAATA 
ATCCTGCATC CTAGTGAACC ATACATTCCT CACTTATGTT TGGAGAGAGG CAAAAAAAGT 
TTCTCTCAAT ACAAAATGCA GATAAGAGAG AGAGGAGACT TCATAAGGGC AGTTAAGAAA 
TCTAGTGACC CAGAAAGACA GGTCAGCTTT GTAAGGAGAC GCTTCCACAA AGATCTCCAT 
TTTACCCCAG TCTTGATGTT GAACTTCTGC CCTGACCTTA CTGCCCTCAG CTCATTAATC 
TATGACCTCC CTACTATAAC CAGAGAAGTC ATTTTCCTTG TTTATCACTC AGGGGAGCTT 
TCGCATAATG TCCGGGATGC TCTCTTGCTG GCTGTTAGGA GTTTACCTGC ACGTACTTTG 
CTGAATCTGG GAAGTGGCAC ATGTGACAGC ATAACCCTGT TTCCGACCAG CAGGCAGTGT 
AGCAATGAGT CATTGGAGGC AGCTTATGAT CATATTAAGA ATGGACAACT CAGTTGCAGT 
TCAAGTAACT TGGTGACTGT TCTGGGCCAT ATCCTGTCTC TTCCAGTGCA TCGTGGATAC 
CCACGGCAGC TCTTTCTCTT CACTGACACA TCTCCAAGGT GTCCTGAAAG AGCCATTGAG 
CTTTTGTGTC ACCATTCAAA GACTACAAGG GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT 
GGCCATATAT TTCTTGAAGA AGCAGCCAGA GTGAGTAGTG GTACGGTGGA GCTGCTAAGT 
GAGGGGGAAA GACTGCATCC CAAGGTGATT AAATCATTAA AGAAGGCGCT GGAGCCAGCT 
CTGAGCGATA TCACTATAGA TTGGTACATG CCAGATACTA TGGAGGCATT GCTGACACCT 
AATGAAATTG CTCCACTTTA CCATGGGGAC TCTCTTATTA GTTATTGTGC TTTGTATCAC 
ACAGCCAGCT TCCGTGGCAA GAGGGACATG AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC 
TCAGTCTTTG AGTCCCAGGA AGATTCCCAG GCCCCAGGGA GCACAGACAC AAGCTGTCTA 
AATCCACCCT CAGGTGCTGA TGATATGGGT CGTGCCCTAC AGGAGATTTC CCAGGAAATA 
TCTTTGGAGT TTTCAGCATG CAGTGAGAAG CCAGATATAA GTGAAGATTC TTTCAGTGAC 
ATCAGGAAAC GCATTGTGCG TTCATCATAT GTAAAGGATC AGTATGTCCT CACCCATTGC 
TCTATCAGTA CTGAGCCAAC TCATGTCCAG ACCCACCCCT CCATCAGCAG TGATCCCACT 
AATACCCCTA CTCGTGGCTC TACTAGCAGT GAATCTGTGG GATCCCATGA TGCTAATGGA 
CACTGCATTT CAGCCGTGAC CTCACAACAA GGACAGAAGA CTGTGTCCGA GGTGGCAGGA 
ATTCATCCTA TCCGAGATCT TGCGTCTTCC AGTAAGGCAC CAGGAACCCA GAGCTCTGAA 
GAACTAATCA AAAGGAAGGC ACTGATGCAG GCCACCATGT CTGGTCGCAG TTTCTCGTCT 
CCCCATGGGG AACTGGATAT GCTGCGGCTG CGTTCTGTCT TGGACAAGGT TTCTCAAAGC 
AGGGAGCCAG ATGGGCGCTG CGCACGTGGA GAAAGCTTAA TGGATTCCTG TCATCTCCTT 
ACCCCTTCCC AGTTGGACTG GGACATGTTG GTTGATGCTC AGTATCTGTT TTCTGCTTCT 
CCTGCTGCAG AAGAAAACAG TGTGATATCT AAAGCAAGCA CTGACACTGT ATGGCAGTGC 
AGGGCTGTGA TACGTGGCCT TTTAGGAAAC AAGAGCCACT CTTGGGAGGC CTCAATAAGT 
CTGCTCTCAC TTATGCACTC TAATGAAGAG GAATCGAATG GTCCCTATGT GGTAGATTCT 
GAATTTGATT TGCATCATCT GGCTGCACAG TCTGTCATTA AAGATAATGA GGATACAGCA 
TGGAGAGTGT GTGACATTGA ACATGGGTCT TCTCGTAGAT ATCGCCTGAA AGCCATACAG 
ACCAGCAAAT CATGCTCTCA AATTTCCACA TTTACTAACC ATGTGTCTGT AGATCTTGCT 
ACCCATGATG TTGTTTCGGA GCACATAAAA GTATGTAGAG GTTGGCAAAA TAATAGAACC 
CACAGCACAA ACCTGGCTAA TTTGCATGAA GGAGAAAACA GAAGCTCAGA AAACATAGAT 
GAAGTCCTCA TGGCTTCAGA TGACACTGCA GATTTCCCAT CATCCCCACA GAGCCTAATC 
TCCACCAGTG GTTCGGTAAA ACAGCGTAGT CAGGAAGGTC AAATTCTCCA TGCCTTTGGC 
TCTCAGAAAC CATCAGAATC ATCGCTGAGT TCCAGGATCA GCAATGGAAA AAGAAAAATC 
CGAAGTATTT CTGGAAAACT TTCGCCCCTG AAACCATTAT GCCTAAGTGA GAGGGAGAAG 
GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC TATCTGCCAA TGATTAGCTT GCAGTCCCCA 
GAAGGATACT TCACACTAAA CAGCAATTTC TCCAGTATGG TTAAAATTTC TCTTGAGCGC 
CTGCTGCGTG CCTCACCCTA TAGCTGCCAC CGTGGTAGCT TAAGTCCAGT GTCTGAACCA 
AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA GGAGGAATGG GAACCTACAA GGGGGGTTCC 
CAACCACAGG TTCCCACTGC ACAAGGTCCA TCCTTTATTC CTGCACATGC TACATCATGC 
CCTGAGGCAC TGTGTCAGCA GGCAGACAGT GGACGTGGAT CTGAAACAGA CCCATGTGAA 
ACATCCCCAT GCCCTTCAGA GGTTTGCACT GATATGGACA CTGACCTAGA GGGTTGCTCC 
TGGGCCACTG CCGTGGCATT AGCATGGCTA GAACATTGCT CTGCAGGATT TTTTACTGAA 
TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA TGGTTAAGAG CACAGGTTTT GCCAGATGGG 
CTACAGCTCT CTGCATTAAA AGGAGCTGCC CGCCAGCTTT TCATCTTGCT CCGTCATTGG 
GATGAGAATC TCAACCTGAA TATGCTGTGC TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa5b2 ( XM_031902162.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758022.1 Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X4, mRNA.

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CloneID	OXa58471
Clone ID Related Accession (Same CDS sequence)	XM_031902162.1
Accession Version	XM_031902162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor A domain-containing protein 5B2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGTC CAACCAGCTG TTTTGGTGGT 
GTAAGTGGAG CAGGAGGAAA TCCATGGGCG TTGCTCAGTG AATCCCCTCC TCTGGTCCTG 
CCTTTACCCT CTGCAGGTTC TCTTGCTGGT GCTCTAGCTG AGCCAGCTAC CAATGCACAG 
CGTTATCAAT TCAGCTTTCG TCTCCAAGTA CAAGGACCTT GTCTTCTGGC AGGAATAGAA 
AGTACTTCTC ATGCTCTCCG TGCTGATGCT AGTCCATTTG CTGTGTCAGC CTCTTCTGTT 
TGGATCAGTT TGGCAGAAGA ACATGACTGT AACCGAGCTT TGGAAATAAT CCTGCATCCT 
AGTGAACCAT ACATTCCTCA CTTATGTTTG GAGAGAGGCA AAAAAAGTTT CTCTCAATAC 
AAAATGCAGA TAAGAGAGAG AGGAGACTTC ATAAGGGCAG TTAAGAAATC TAGTGACCCA 
GAAAGACAGG TCAGCTTTGT AAGGAGACGC TTCCACAAAG ATCTCCATTT TACCCCAGTC 
TTGATGTTGA ACTTCTGCCC TGACCTTACT GCCCTCAGCT CATTAATCTA TGACCTCCCT 
ACTATAACCA GAGAAGTCAT TTTCCTTGTT TATCACTCAG GGGAGCTTTC GCATAATGTC 
CGGGATGCTC TCTTGCTGGC TGTTAGGAGT TTACCTGCAC GTACTTTGCT GAATCTGGGA 
AGTGGCACAT GTGACAGCAT AACCCTGTTT CCGACCAGCA GGCAGTGTAG CAATGAGTCA 
TTGGAGGCAG CTTATGATCA TATTAAGAAT GGACAACTCA GTTGCAGTTC AAGTAACTTG 
GTGACTGTTC TGGGCCATAT CCTGTCTCTT CCAGTGCATC GTGGATACCC ACGGCAGCTC 
TTTCTCTTCA CTGACACATC TCCAAGGTGT CCTGAAAGAG CCATTGAGCT TTTGTGTCAC 
CATTCAAAGA CTACAAGGGT GTTTGGTTTT GGTCTTGGTG TCAAAGCTGG CCATATATTT 
CTTGAAGAAG CAGCCAGAGT GAGTAGTGGT ACGGTGGAGC TGCTAAGTGA GGGGGAAAGA 
CTGCATCCCA AGGTGATTAA ATCATTAAAG AAGGCGCTGG AGCCAGCTCT GAGCGATATC 
ACTATAGATT GGTACATGCC AGATACTATG GAGGCATTGC TGACACCTAA TGAAATTGCT 
CCACTTTACC ATGGGGACTC TCTTATTAGT TATTGTGCTT TGTATCACAC AGCCAGCTTC 
CGTGGCAAGA GGGACATGAG AAAGAGTGCC AGGTGTGGCT CTTTTAGCTC AGTCTTTGAG 
TCCCAGGAAG ATTCCCAGGC CCCAGGGAGC ACAGACACAA GCTGTCTAAA TCCACCCTCA 
GGTGCTGATG ATATGGGTCG TGCCCTACAG GAGATTTCCC AGGAAATATC TTTGGAGTTT 
TCAGCATGCA GTGAGAAGCC AGATATAAGT GAAGATTCTT TCAGTGACAT CAGGAAACGC 
ATTGTGCGTT CATCATATGT AAAGGATCAG TATGTCCTCA CCCATTGCTC TATCAGTACT 
GAGCCAACTC ATGTCCAGAC CCACCCCTCC ATCAGCAGTG ATCCCACTAA TACCCCTACT 
CGTGGCTCTA CTAGCAGTGA ATCTGTGGGA TCCCATGATG CTAATGGACA CTGCATTTCA 
GCCGTGACCT CACAACAAGG ACAGAAGACT GTGTCCGAGG TGGCAGGAAT TCATCCTATC 
CGAGATCTTG CGTCTTCCAG TAAGGCACCA GGAACCCAGA GCTCTGAAGA ACTAATCAAA 
AGGAAGGCAC TGATGCAGGC CACCATGTCT GGTCGCAGTT TCTCGTCTCC CCATGGGGAA 
CTGGATATGC TGCGGCTGCG TTCTGTCTTG GACAAGGTTT CTCAAAGCAG GGAGCCAGAT 
GGGCGCTGCG CACGTGGAGA AAGCTTAATG GATTCCTGTC ATCTCCTTAC CCCTTCCCAG 
TTGGACTGGG ACATGTTGGT TGATGCTCAG TATCTGTTTT CTGCTTCTCC TGCTGCAGAA 
GAAAACAGTG TGATATCTAA AGCAAGCACT GACACTGTAT GGCAGTGCAG GGCTGTGATA 
CGTGGCCTTT TAGGAAACAA GAGCCACTCT TGGGAGGCCT CAATAAGTCT GCTCTCACTT 
ATGCACTCTA ATGAAGAGGA ATCGAATGGT CCCTATGTGG TAGATTCTGA ATTTGATTTG 
CATCATCTGG CTGCACAGTC TGTCATTAAA GATAATGAGG ATACAGCATG GAGAGTGTGT 
GACATTGAAC ATGGGTCTTC TCGTAGATAT CGCCTGAAAG CCATACAGAC CAGCAAATCA 
TGCTCTCAAA TTTCCACATT TACTAACCAT GTGTCTGTAG ATCTTGCTAC CCATGATGTT 
GTTTCGGAGC ACATAAAAGT ATGTAGAGGT TGGCAAAATA ATAGAACCCA CAGCACAAAC 
CTGGCTAATT TGCATGAAGG AGAAAACAGA AGCTCAGAAA ACATAGATGA AGTCCTCATG 
GCTTCAGATG ACACTGCAGA TTTCCCATCA TCCCCACAGA GCCTAATCTC CACCAGTGGT 
TCGGTAAAAC AGCGTAGTCA GGAAGGTCAA ATTCTCCATG CCTTTGGCTC TCAGAAACCA 
TCAGAATCAT CGCTGAGTTC CAGGATCAGC AATGGAAAAA GAAAAATCCG AAGTATTTCT 
GGAAAACTTT CGCCCCTGAA ACCATTATGC CTAAGTGAGA GGGAGAAGGA GAGAAGCGAA 
GAGTCTGGGT TTCAGGACTA TCTGCCAATG ATTAGCTTGC AGTCCCCAGA AGGATACTTC 
ACACTAAACA GCAATTTCTC CAGTATGGTT AAAATTTCTC TTGAGCGCCT GCTGCGTGCC 
TCACCCTATA GCTGCCACCG TGGTAGCTTA AGTCCAGTGT CTGAACCAAG CCTTCTAGAG 
CAGGGGCACT TCCTTAAAGG AGGAATGGGA ACCTACAAGG GGGGTTCCCA ACCACAGGTT 
CCCACTGCAC AAGGTCCATC CTTTATTCCT GCACATGCTA CATCATGCCC TGAGGCACTG 
TGTCAGCAGG CAGACAGTGG ACGTGGATCT GAAACAGACC CATGTGAAAC ATCCCCATGC 
CCTTCAGAGG TTTGCACTGA TATGGACACT GACCTAGAGG GTTGCTCCTG GGCCACTGCC 
GTGGCATTAG CATGGCTAGA ACATTGCTCT GCAGGATTTT TTACTGAATG GGAGGTACTA 
GCTGCAAAAG CAGAGGTATG GTTAAGAGCA CAGGTTTTGC CAGATGGGCT ACAGCTCTCT 
GCATTAAAAG GAGCTGCCCG CCAGCTTTTC ATCTTGCTCC GTCATTGGGA TGAGAATCTC 
AACCTGAATA TGCTGTGCTA CAAGCCAGGT AATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758022.1
CDS	602..4480
Translation	MPGLYNALSWVQLPLESSSVTAYANGYCMGLNAHLTYLNPGNETVQAVFIYLLDDGEVVTSFEAVTGNQKVQAQVQSQSQMEECCTKFPWDFLPCFCPNGHLILDSNSAHSVFILSVGEVPPSAALSVYLRSSRELKTLPNGAVRVILNSTLTPFAPSPGVPSEEGECLCDDSPTSCFGGVSGAGGNPWALLSESPPLVLPLPSAGSLAGALAEPATNAQRYQFSFRLQVQGPCLLAGIESTSHALRADASPFAVSASSVWISLAEEHDCNRALEIILHPSEPYIPHLCLERGKKSFSQYKMQIRERGDFIRAVKKSSDPERQVSFVRRRFHKDLHFTPVLMLNFCPDLTALSSLIYDLPTITREVIFLVYHSGELSHNVRDALLLAVRSLPARTLLNLGSGTCDSITLFPTSRQCSNESLEAAYDHIKNGQLSCSSSNLVTVLGHILSLPVHRGYPRQLFLFTDTSPRCPERAIELLCHHSKTTRVFGFGLGVKAGHIFLEEAARVSSGTVELLSEGERLHPKVIKSLKKALEPALSDITIDWYMPDTMEALLTPNEIAPLYHGDSLISYCALYHTASFRGKRDMRKSARCGSFSSVFESQEDSQAPGSTDTSCLNPPSGADDMGRALQEISQEISLEFSACSEKPDISEDSFSDIRKRIVRSSYVKDQYVLTHCSISTEPTHVQTHPSISSDPTNTPTRGSTSSESVGSHDANGHCISAVTSQQGQKTVSEVAGIHPIRDLASSSKAPGTQSSEELIKRKALMQATMSGRSFSSPHGELDMLRLRSVLDKVSQSREPDGRCARGESLMDSCHLLTPSQLDWDMLVDAQYLFSASPAAEENSVISKASTDTVWQCRAVIRGLLGNKSHSWEASISLLSLMHSNEEESNGPYVVDSEFDLHHLAAQSVIKDNEDTAWRVCDIEHGSSRRYRLKAIQTSKSCSQISTFTNHVSVDLATHDVVSEHIKVCRGWQNNRTHSTNLANLHEGENRSSENIDEVLMASDDTADFPSSPQSLISTSGSVKQRSQEGQILHAFGSQKPSESSLSSRISNGKRKIRSISGKLSPLKPLCLSEREKERSEESGFQDYLPMISLQSPEGYFTLNSNFSSMVKISLERLLRASPYSCHRGSLSPVSEPSLLEQGHFLKGGMGTYKGGSQPQVPTAQGPSFIPAHATSCPEALCQQADSGRGSETDPCETSPCPSEVCTDMDTDLEGCSWATAVALAWLEHCSAGFFTEWEVLAAKAEVWLRAQVLPDGLQLSALKGAARQLFILLRHWDENLNLNMLCYKPGNV

Target ORF information:

RefSeq Version	XM_031902162.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902162.1

ORF Insert Sequence:

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGTC CAACCAGCTG TTTTGGTGGT 
GTAAGTGGAG CAGGAGGAAA TCCATGGGCG TTGCTCAGTG AATCCCCTCC TCTGGTCCTG 
CCTTTACCCT CTGCAGGTTC TCTTGCTGGT GCTCTAGCTG AGCCAGCTAC CAATGCACAG 
CGTTATCAAT TCAGCTTTCG TCTCCAAGTA CAAGGACCTT GTCTTCTGGC AGGAATAGAA 
AGTACTTCTC ATGCTCTCCG TGCTGATGCT AGTCCATTTG CTGTGTCAGC CTCTTCTGTT 
TGGATCAGTT TGGCAGAAGA ACATGACTGT AACCGAGCTT TGGAAATAAT CCTGCATCCT 
AGTGAACCAT ACATTCCTCA CTTATGTTTG GAGAGAGGCA AAAAAAGTTT CTCTCAATAC 
AAAATGCAGA TAAGAGAGAG AGGAGACTTC ATAAGGGCAG TTAAGAAATC TAGTGACCCA 
GAAAGACAGG TCAGCTTTGT AAGGAGACGC TTCCACAAAG ATCTCCATTT TACCCCAGTC 
TTGATGTTGA ACTTCTGCCC TGACCTTACT GCCCTCAGCT CATTAATCTA TGACCTCCCT 
ACTATAACCA GAGAAGTCAT TTTCCTTGTT TATCACTCAG GGGAGCTTTC GCATAATGTC 
CGGGATGCTC TCTTGCTGGC TGTTAGGAGT TTACCTGCAC GTACTTTGCT GAATCTGGGA 
AGTGGCACAT GTGACAGCAT AACCCTGTTT CCGACCAGCA GGCAGTGTAG CAATGAGTCA 
TTGGAGGCAG CTTATGATCA TATTAAGAAT GGACAACTCA GTTGCAGTTC AAGTAACTTG 
GTGACTGTTC TGGGCCATAT CCTGTCTCTT CCAGTGCATC GTGGATACCC ACGGCAGCTC 
TTTCTCTTCA CTGACACATC TCCAAGGTGT CCTGAAAGAG CCATTGAGCT TTTGTGTCAC 
CATTCAAAGA CTACAAGGGT GTTTGGTTTT GGTCTTGGTG TCAAAGCTGG CCATATATTT 
CTTGAAGAAG CAGCCAGAGT GAGTAGTGGT ACGGTGGAGC TGCTAAGTGA GGGGGAAAGA 
CTGCATCCCA AGGTGATTAA ATCATTAAAG AAGGCGCTGG AGCCAGCTCT GAGCGATATC 
ACTATAGATT GGTACATGCC AGATACTATG GAGGCATTGC TGACACCTAA TGAAATTGCT 
CCACTTTACC ATGGGGACTC TCTTATTAGT TATTGTGCTT TGTATCACAC AGCCAGCTTC 
CGTGGCAAGA GGGACATGAG AAAGAGTGCC AGGTGTGGCT CTTTTAGCTC AGTCTTTGAG 
TCCCAGGAAG ATTCCCAGGC CCCAGGGAGC ACAGACACAA GCTGTCTAAA TCCACCCTCA 
GGTGCTGATG ATATGGGTCG TGCCCTACAG GAGATTTCCC AGGAAATATC TTTGGAGTTT 
TCAGCATGCA GTGAGAAGCC AGATATAAGT GAAGATTCTT TCAGTGACAT CAGGAAACGC 
ATTGTGCGTT CATCATATGT AAAGGATCAG TATGTCCTCA CCCATTGCTC TATCAGTACT 
GAGCCAACTC ATGTCCAGAC CCACCCCTCC ATCAGCAGTG ATCCCACTAA TACCCCTACT 
CGTGGCTCTA CTAGCAGTGA ATCTGTGGGA TCCCATGATG CTAATGGACA CTGCATTTCA 
GCCGTGACCT CACAACAAGG ACAGAAGACT GTGTCCGAGG TGGCAGGAAT TCATCCTATC 
CGAGATCTTG CGTCTTCCAG TAAGGCACCA GGAACCCAGA GCTCTGAAGA ACTAATCAAA 
AGGAAGGCAC TGATGCAGGC CACCATGTCT GGTCGCAGTT TCTCGTCTCC CCATGGGGAA 
CTGGATATGC TGCGGCTGCG TTCTGTCTTG GACAAGGTTT CTCAAAGCAG GGAGCCAGAT 
GGGCGCTGCG CACGTGGAGA AAGCTTAATG GATTCCTGTC ATCTCCTTAC CCCTTCCCAG 
TTGGACTGGG ACATGTTGGT TGATGCTCAG TATCTGTTTT CTGCTTCTCC TGCTGCAGAA 
GAAAACAGTG TGATATCTAA AGCAAGCACT GACACTGTAT GGCAGTGCAG GGCTGTGATA 
CGTGGCCTTT TAGGAAACAA GAGCCACTCT TGGGAGGCCT CAATAAGTCT GCTCTCACTT 
ATGCACTCTA ATGAAGAGGA ATCGAATGGT CCCTATGTGG TAGATTCTGA ATTTGATTTG 
CATCATCTGG CTGCACAGTC TGTCATTAAA GATAATGAGG ATACAGCATG GAGAGTGTGT 
GACATTGAAC ATGGGTCTTC TCGTAGATAT CGCCTGAAAG CCATACAGAC CAGCAAATCA 
TGCTCTCAAA TTTCCACATT TACTAACCAT GTGTCTGTAG ATCTTGCTAC CCATGATGTT 
GTTTCGGAGC ACATAAAAGT ATGTAGAGGT TGGCAAAATA ATAGAACCCA CAGCACAAAC 
CTGGCTAATT TGCATGAAGG AGAAAACAGA AGCTCAGAAA ACATAGATGA AGTCCTCATG 
GCTTCAGATG ACACTGCAGA TTTCCCATCA TCCCCACAGA GCCTAATCTC CACCAGTGGT 
TCGGTAAAAC AGCGTAGTCA GGAAGGTCAA ATTCTCCATG CCTTTGGCTC TCAGAAACCA 
TCAGAATCAT CGCTGAGTTC CAGGATCAGC AATGGAAAAA GAAAAATCCG AAGTATTTCT 
GGAAAACTTT CGCCCCTGAA ACCATTATGC CTAAGTGAGA GGGAGAAGGA GAGAAGCGAA 
GAGTCTGGGT TTCAGGACTA TCTGCCAATG ATTAGCTTGC AGTCCCCAGA AGGATACTTC 
ACACTAAACA GCAATTTCTC CAGTATGGTT AAAATTTCTC TTGAGCGCCT GCTGCGTGCC 
TCACCCTATA GCTGCCACCG TGGTAGCTTA AGTCCAGTGT CTGAACCAAG CCTTCTAGAG 
CAGGGGCACT TCCTTAAAGG AGGAATGGGA ACCTACAAGG GGGGTTCCCA ACCACAGGTT 
CCCACTGCAC AAGGTCCATC CTTTATTCCT GCACATGCTA CATCATGCCC TGAGGCACTG 
TGTCAGCAGG CAGACAGTGG ACGTGGATCT GAAACAGACC CATGTGAAAC ATCCCCATGC 
CCTTCAGAGG TTTGCACTGA TATGGACACT GACCTAGAGG GTTGCTCCTG GGCCACTGCC 
GTGGCATTAG CATGGCTAGA ACATTGCTCT GCAGGATTTT TTACTGAATG GGAGGTACTA 
GCTGCAAAAG CAGAGGTATG GTTAAGAGCA CAGGTTTTGC CAGATGGGCT ACAGCTCTCT 
GCATTAAAAG GAGCTGCCCG CCAGCTTTTC ATCTTGCTCC GTCATTGGGA TGAGAATCTC 
AACCTGAATA TGCTGTGCTA CAAGCCAGGT AATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa5b2 ( XM_002935141.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935187.2 Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa10198
Clone ID Related Accession (Same CDS sequence)	XM_002935141.4
Accession Version	XM_002935141.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor A domain-containing protein 5B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935141.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCAG AGCATATGAA GGGTAGCAGC ATGCCAGGTC TTTATAATGC CTTATCATGG 
GTTCAACTCC CTCTCGAGTC GTCCTCTGTA ACTGCCTATG CCAATGGATA CTGCATGGGT 
CTCAATGCCC ACCTCACATA TCTTAACCCA GGAAATGAAA CTGTTCAAGC TGTTTTTATA 
TATCTGCTGG ATGATGGAGA GGTGGTAACT AGCTTTGAGG CTGTGACTGG CAACCAAAAA 
GTACAAGCCC AGGTCCAAAG TCAAAGCCAA ATGGAGGAAT GCTGCACCAA GTTCCCTTGG 
GATTTTCTGC CTTGTTTTTG TCCCAATGGT CACCTTATCC TGGATTCAAA TAGTGCACAT 
AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT CCTCCTTCTG CAGCACTAAG TGTGTACCTT 
AGGAGCAGCA GAGAGCTAAA GACTCTTCCA AATGGAGCAG TGCGTGTGAT CCTCAACTCC 
ACACTCACAC CCTTTGCCCC TTCTCCAGGG GTACCCAGTG AAGAGGGGGA GTGTCTGTGT 
GACGACAGGT TGTGCTTATG TCCAACCAGC TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA 
AATCCATGGG CGTTGCTCAG TGAATCCCCT CCTCTGGTCC TGCCTTTACC CTCTGCAGGT 
TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT ACCAATGCAC AGCGTTATCA ATTCAGCTTT 
CGTCTCCAAG TACAAGGACC TTGTCTTCTG GCAGGAATAG AAAGTACTTC TCATGCTCTC 
CGTGCTGATG CTAGTCCATT TGCTGTGTCA GCCTCTTCTG TTTGGATCAG TTTGGCAGAA 
GAACATGACT GTAACCGAGC TTTGGAAATA ATCCTGCATC CTAGTGAACC ATACATTCCT 
CACTTATGTT TGGAGAGAGG CAAAAAAAGT TTCTCTCAAT ACAAAATGCA GATAAGAGAG 
AGAGGAGACT TCATAAGGGC AGTTAAGAAA TCTAGTGACC CAGAAAGACA GGTCAGCTTT 
GTAAGGAGAC GCTTCCACAA AGATCTCCAT TTTACCCCAG TCTTGATGTT GAACTTCTGC 
CCTGACCTTA CTGCCCTCAG CTCATTAATC TATGACCTCC CTACTATAAC CAGAGAAGTC 
ATTTTCCTTG TTTATCACTC AGGGGAGCTT TCGCATAATG TCCGGGATGC TCTCTTGCTG 
GCTGTTAGGA GTTTACCTGC ACGTACTTTG CTGAATCTGG GAAGTGGCAC ATGTGACAGC 
ATAACCCTGT TTCCGACCAG CAGGCAGTGT AGCAATGAGT CATTGGAGGC AGCTTATGAT 
CATATTAAGA ATGGACAACT CAGTTGCAGT TCAAGTAACT TGGTGACTGT TCTGGGCCAT 
ATCCTGTCTC TTCCAGTGCA TCGTGGATAC CCACGGCAGC TCTTTCTCTT CACTGACACA 
TCTCCAAGGT GTCCTGAAAG AGCCATTGAG CTTTTGTGTC ACCATTCAAA GACTACAAGG 
GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT GGCCATATAT TTCTTGAAGA AGCAGCCAGA 
GTGAGTAGTG GTACGGTGGA GCTGCTAAGT GAGGGGGAAA GACTGCATCC CAAGGTGATT 
AAATCATTAA AGAAGGCGCT GGAGCCAGCT CTGAGCGATA TCACTATAGA TTGGTACATG 
CCAGATACTA TGGAGGCATT GCTGACACCT AATGAAATTG CTCCACTTTA CCATGGGGAC 
TCTCTTATTA GTTATTGTGC TTTGTATCAC ACAGCCAGCT TCCGTGGCAA GAGGGACATG 
AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC TCAGTCTTTG AGTCCCAGGA AGATTCCCAG 
GCCCCAGGGA GCACAGACAC AAGCTGTCTA AATCCACCCT CAGGTGCTGA TGATATGGGT 
CGTGCCCTAC AGGAGATTTC CCAGGAAATA TCTTTGGAGT TTTCAGCATG CAGTGAGAAG 
CCAGATATAA GTGAAGATTC TTTCAGTGAC ATCAGGAAAC GCATTGTGCG TTCATCATAT 
GTAAAGGATC AGTATGTCCT CACCCATTGC TCTATCAGTA CTGAGCCAAC TCATGTCCAG 
ACCCACCCCT CCATCAGCAG TGATCCCACT AATACCCCTA CTCGTGGCTC TACTAGCAGT 
GAATCTGTGG GATCCCATGA TGCTAATGGA CACTGCATTT CAGCCGTGAC CTCACAACAA 
GGACAGAAGA CTGTGTCCGA GGTGGCAGGA ATTCATCCTA TCCGAGATCT TGCGTCTTCC 
AGTAAGGCAC CAGGAACCCA GAGCTCTGAA GAACTAATCA AAAGGAAGGC ACTGATGCAG 
GCCACCATGT CTGGTCGCAG TTTCTCGTCT CCCCATGGGG AACTGGATAT GCTGCGGCTG 
CGTTCTGTCT TGGACAAGGT TTCTCAAAGC AGGGAGCCAG ATGGGCGCTG CGCACGTGGA 
GAAAGCTTAA TGGATTCCTG TCATCTCCTT ACCCCTTCCC AGTTGGACTG GGACATGTTG 
GTTGATGCTC AGTATCTGTT TTCTGCTTCT CCTGCTGCAG AAGAAAACAG TGTGATATCT 
AAAGCAAGCA CTGACACTGT ATGGCAGTGC AGGGCTGTGA TACGTGGCCT TTTAGGAAAC 
AAGAGCCACT CTTGGGAGGC CTCAATAAGT CTGCTCTCAC TTATGCACTC TAATGAAGAG 
GAATCGAATG GTCCCTATGT GGTAGATTCT GAATTTGATT TGCATCATCT GGCTGCACAG 
TCTGTCATTA AAGATAATGA GGATACAGCA TGGAGAGTGT GTGACATTGA ACATGGGTCT 
TCTCGTAGAT ATCGCCTGAA AGCCATACAG ACCAGCAAAT CATGCTCTCA AATTTCCACA 
TTTACTAACC ATGTGTCTGT AGATCTTGCT ACCCATGATG TTGTTTCGGA GCACATAAAA 
GTATGTAGAG GTTGGCAAAA TAATAGAACC CACAGCACAA ACCTGGCTAA TTTGCATGAA 
GGAGAAAACA GAAGCTCAGA AAACATAGAT GAAGTCCTCA TGGCTTCAGA TGACACTGCA 
GATTTCCCAT CATCCCCACA GAGCCTAATC TCCACCAGTG GTTCGGTAAA ACAGCGTAGT 
CAGGAAGGTC AAATTCTCCA TGCCTTTGGC TCTCAGAAAC CATCAGAATC ATCGCTGAGT 
TCCAGGATCA GCAATGGAAA AAGAAAAATC CGAAGTATTT CTGGAAAACT TTCGCCCCTG 
AAACCATTAT GCCTAAGTGA GAGGGAGAAG GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC 
TATCTGCCAA TGATTAGCTT GCAGTCCCCA GAAGGATACT TCACACTAAA CAGCAATTTC 
TCCAGTATGG TTAAAATTTC TCTTGAGCGC CTGCTGCGTG CCTCACCCTA TAGCTGCCAC 
CGTGGTAGCT TAAGTCCAGT GTCTGAACCA AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA 
GGAGGAATGG GAACCTACAA GGGGGGTTCC CAACCACAGG TTCCCACTGC ACAAGGTCCA 
TCCTTTATTC CTGCACATGC TACATCATGC CCTGAGGCAC TGTGTCAGCA GGCAGACAGT 
GGACGTGGAT CTGAAACAGA CCCATGTGAA ACATCCCCAT GCCCTTCAGA GGTTTGCACT 
GATATGGACA CTGACCTAGA GGGTTGCTCC TGGGCCACTG CCGTGGCATT AGCATGGCTA 
GAACATTGCT CTGCAGGATT TTTTACTGAA TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA 
TGGTTAAGAG CACAGGTTTT GCCAGATGGG CTACAGCTCT CTGCATTAAA AGGAGCTGCC 
CGCCAGCTTT TCATCTTGCT CCGTCATTGG GATGAGAATC TCAACCTGAA TATGCTGTGC 
TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935187.2
CDS	39..3959
Translation	MSAEHMKGSSMPGLYNALSWVQLPLESSSVTAYANGYCMGLNAHLTYLNPGNETVQAVFIYLLDDGEVVTSFEAVTGNQKVQAQVQSQSQMEECCTKFPWDFLPCFCPNGHLILDSNSAHSVFILSVGEVPPSAALSVYLRSSRELKTLPNGAVRVILNSTLTPFAPSPGVPSEEGECLCDDRLCLCPTSCFGGVSGAGGNPWALLSESPPLVLPLPSAGSLAGALAEPATNAQRYQFSFRLQVQGPCLLAGIESTSHALRADASPFAVSASSVWISLAEEHDCNRALEIILHPSEPYIPHLCLERGKKSFSQYKMQIRERGDFIRAVKKSSDPERQVSFVRRRFHKDLHFTPVLMLNFCPDLTALSSLIYDLPTITREVIFLVYHSGELSHNVRDALLLAVRSLPARTLLNLGSGTCDSITLFPTSRQCSNESLEAAYDHIKNGQLSCSSSNLVTVLGHILSLPVHRGYPRQLFLFTDTSPRCPERAIELLCHHSKTTRVFGFGLGVKAGHIFLEEAARVSSGTVELLSEGERLHPKVIKSLKKALEPALSDITIDWYMPDTMEALLTPNEIAPLYHGDSLISYCALYHTASFRGKRDMRKSARCGSFSSVFESQEDSQAPGSTDTSCLNPPSGADDMGRALQEISQEISLEFSACSEKPDISEDSFSDIRKRIVRSSYVKDQYVLTHCSISTEPTHVQTHPSISSDPTNTPTRGSTSSESVGSHDANGHCISAVTSQQGQKTVSEVAGIHPIRDLASSSKAPGTQSSEELIKRKALMQATMSGRSFSSPHGELDMLRLRSVLDKVSQSREPDGRCARGESLMDSCHLLTPSQLDWDMLVDAQYLFSASPAAEENSVISKASTDTVWQCRAVIRGLLGNKSHSWEASISLLSLMHSNEEESNGPYVVDSEFDLHHLAAQSVIKDNEDTAWRVCDIEHGSSRRYRLKAIQTSKSCSQISTFTNHVSVDLATHDVVSEHIKVCRGWQNNRTHSTNLANLHEGENRSSENIDEVLMASDDTADFPSSPQSLISTSGSVKQRSQEGQILHAFGSQKPSESSLSSRISNGKRKIRSISGKLSPLKPLCLSEREKERSEESGFQDYLPMISLQSPEGYFTLNSNFSSMVKISLERLLRASPYSCHRGSLSPVSEPSLLEQGHFLKGGMGTYKGGSQPQVPTAQGPSFIPAHATSCPEALCQQADSGRGSETDPCETSPCPSEVCTDMDTDLEGCSWATAVALAWLEHCSAGFFTEWEVLAAKAEVWLRAQVLPDGLQLSALKGAARQLFILLRHWDENLNLNMLCYKPGNV

Target ORF information:

RefSeq Version	XM_002935141.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935141.4

ORF Insert Sequence:

ATGAGTGCAG AGCATATGAA GGGTAGCAGC ATGCCAGGTC TTTATAATGC CTTATCATGG 
GTTCAACTCC CTCTCGAGTC GTCCTCTGTA ACTGCCTATG CCAATGGATA CTGCATGGGT 
CTCAATGCCC ACCTCACATA TCTTAACCCA GGAAATGAAA CTGTTCAAGC TGTTTTTATA 
TATCTGCTGG ATGATGGAGA GGTGGTAACT AGCTTTGAGG CTGTGACTGG CAACCAAAAA 
GTACAAGCCC AGGTCCAAAG TCAAAGCCAA ATGGAGGAAT GCTGCACCAA GTTCCCTTGG 
GATTTTCTGC CTTGTTTTTG TCCCAATGGT CACCTTATCC TGGATTCAAA TAGTGCACAT 
AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT CCTCCTTCTG CAGCACTAAG TGTGTACCTT 
AGGAGCAGCA GAGAGCTAAA GACTCTTCCA AATGGAGCAG TGCGTGTGAT CCTCAACTCC 
ACACTCACAC CCTTTGCCCC TTCTCCAGGG GTACCCAGTG AAGAGGGGGA GTGTCTGTGT 
GACGACAGGT TGTGCTTATG TCCAACCAGC TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA 
AATCCATGGG CGTTGCTCAG TGAATCCCCT CCTCTGGTCC TGCCTTTACC CTCTGCAGGT 
TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT ACCAATGCAC AGCGTTATCA ATTCAGCTTT 
CGTCTCCAAG TACAAGGACC TTGTCTTCTG GCAGGAATAG AAAGTACTTC TCATGCTCTC 
CGTGCTGATG CTAGTCCATT TGCTGTGTCA GCCTCTTCTG TTTGGATCAG TTTGGCAGAA 
GAACATGACT GTAACCGAGC TTTGGAAATA ATCCTGCATC CTAGTGAACC ATACATTCCT 
CACTTATGTT TGGAGAGAGG CAAAAAAAGT TTCTCTCAAT ACAAAATGCA GATAAGAGAG 
AGAGGAGACT TCATAAGGGC AGTTAAGAAA TCTAGTGACC CAGAAAGACA GGTCAGCTTT 
GTAAGGAGAC GCTTCCACAA AGATCTCCAT TTTACCCCAG TCTTGATGTT GAACTTCTGC 
CCTGACCTTA CTGCCCTCAG CTCATTAATC TATGACCTCC CTACTATAAC CAGAGAAGTC 
ATTTTCCTTG TTTATCACTC AGGGGAGCTT TCGCATAATG TCCGGGATGC TCTCTTGCTG 
GCTGTTAGGA GTTTACCTGC ACGTACTTTG CTGAATCTGG GAAGTGGCAC ATGTGACAGC 
ATAACCCTGT TTCCGACCAG CAGGCAGTGT AGCAATGAGT CATTGGAGGC AGCTTATGAT 
CATATTAAGA ATGGACAACT CAGTTGCAGT TCAAGTAACT TGGTGACTGT TCTGGGCCAT 
ATCCTGTCTC TTCCAGTGCA TCGTGGATAC CCACGGCAGC TCTTTCTCTT CACTGACACA 
TCTCCAAGGT GTCCTGAAAG AGCCATTGAG CTTTTGTGTC ACCATTCAAA GACTACAAGG 
GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT GGCCATATAT TTCTTGAAGA AGCAGCCAGA 
GTGAGTAGTG GTACGGTGGA GCTGCTAAGT GAGGGGGAAA GACTGCATCC CAAGGTGATT 
AAATCATTAA AGAAGGCGCT GGAGCCAGCT CTGAGCGATA TCACTATAGA TTGGTACATG 
CCAGATACTA TGGAGGCATT GCTGACACCT AATGAAATTG CTCCACTTTA CCATGGGGAC 
TCTCTTATTA GTTATTGTGC TTTGTATCAC ACAGCCAGCT TCCGTGGCAA GAGGGACATG 
AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC TCAGTCTTTG AGTCCCAGGA AGATTCCCAG 
GCCCCAGGGA GCACAGACAC AAGCTGTCTA AATCCACCCT CAGGTGCTGA TGATATGGGT 
CGTGCCCTAC AGGAGATTTC CCAGGAAATA TCTTTGGAGT TTTCAGCATG CAGTGAGAAG 
CCAGATATAA GTGAAGATTC TTTCAGTGAC ATCAGGAAAC GCATTGTGCG TTCATCATAT 
GTAAAGGATC AGTATGTCCT CACCCATTGC TCTATCAGTA CTGAGCCAAC TCATGTCCAG 
ACCCACCCCT CCATCAGCAG TGATCCCACT AATACCCCTA CTCGTGGCTC TACTAGCAGT 
GAATCTGTGG GATCCCATGA TGCTAATGGA CACTGCATTT CAGCCGTGAC CTCACAACAA 
GGACAGAAGA CTGTGTCCGA GGTGGCAGGA ATTCATCCTA TCCGAGATCT TGCGTCTTCC 
AGTAAGGCAC CAGGAACCCA GAGCTCTGAA GAACTAATCA AAAGGAAGGC ACTGATGCAG 
GCCACCATGT CTGGTCGCAG TTTCTCGTCT CCCCATGGGG AACTGGATAT GCTGCGGCTG 
CGTTCTGTCT TGGACAAGGT TTCTCAAAGC AGGGAGCCAG ATGGGCGCTG CGCACGTGGA 
GAAAGCTTAA TGGATTCCTG TCATCTCCTT ACCCCTTCCC AGTTGGACTG GGACATGTTG 
GTTGATGCTC AGTATCTGTT TTCTGCTTCT CCTGCTGCAG AAGAAAACAG TGTGATATCT 
AAAGCAAGCA CTGACACTGT ATGGCAGTGC AGGGCTGTGA TACGTGGCCT TTTAGGAAAC 
AAGAGCCACT CTTGGGAGGC CTCAATAAGT CTGCTCTCAC TTATGCACTC TAATGAAGAG 
GAATCGAATG GTCCCTATGT GGTAGATTCT GAATTTGATT TGCATCATCT GGCTGCACAG 
TCTGTCATTA AAGATAATGA GGATACAGCA TGGAGAGTGT GTGACATTGA ACATGGGTCT 
TCTCGTAGAT ATCGCCTGAA AGCCATACAG ACCAGCAAAT CATGCTCTCA AATTTCCACA 
TTTACTAACC ATGTGTCTGT AGATCTTGCT ACCCATGATG TTGTTTCGGA GCACATAAAA 
GTATGTAGAG GTTGGCAAAA TAATAGAACC CACAGCACAA ACCTGGCTAA TTTGCATGAA 
GGAGAAAACA GAAGCTCAGA AAACATAGAT GAAGTCCTCA TGGCTTCAGA TGACACTGCA 
GATTTCCCAT CATCCCCACA GAGCCTAATC TCCACCAGTG GTTCGGTAAA ACAGCGTAGT 
CAGGAAGGTC AAATTCTCCA TGCCTTTGGC TCTCAGAAAC CATCAGAATC ATCGCTGAGT 
TCCAGGATCA GCAATGGAAA AAGAAAAATC CGAAGTATTT CTGGAAAACT TTCGCCCCTG 
AAACCATTAT GCCTAAGTGA GAGGGAGAAG GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC 
TATCTGCCAA TGATTAGCTT GCAGTCCCCA GAAGGATACT TCACACTAAA CAGCAATTTC 
TCCAGTATGG TTAAAATTTC TCTTGAGCGC CTGCTGCGTG CCTCACCCTA TAGCTGCCAC 
CGTGGTAGCT TAAGTCCAGT GTCTGAACCA AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA 
GGAGGAATGG GAACCTACAA GGGGGGTTCC CAACCACAGG TTCCCACTGC ACAAGGTCCA 
TCCTTTATTC CTGCACATGC TACATCATGC CCTGAGGCAC TGTGTCAGCA GGCAGACAGT 
GGACGTGGAT CTGAAACAGA CCCATGTGAA ACATCCCCAT GCCCTTCAGA GGTTTGCACT 
GATATGGACA CTGACCTAGA GGGTTGCTCC TGGGCCACTG CCGTGGCATT AGCATGGCTA 
GAACATTGCT CTGCAGGATT TTTTACTGAA TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA 
TGGTTAAGAG CACAGGTTTT GCCAGATGGG CTACAGCTCT CTGCATTAAA AGGAGCTGCC 
CGCCAGCTTT TCATCTTGCT CCGTCATTGG GATGAGAATC TCAACCTGAA TATGCTGTGC 
TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa5b2 ( XM_012963368.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818822.1 Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X2, mRNA.

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CloneID	OXa34064
Clone ID Related Accession (Same CDS sequence)	XM_018094413.2 , XM_012963368.2 , XM_012963369.2 , XM_012963368.3 , XM_012963369.3 , XM_018094413.1
Accession Version	XM_012963368.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor A domain-containing protein 5B2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963368.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGGT TGTGCTTATG TCCAACCAGC 
TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA AATCCATGGG CGTTGCTCAG TGAATCCCCT 
CCTCTGGTCC TGCCTTTACC CTCTGCAGGT TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT 
ACCAATGCAC AGCGTTATCA ATTCAGCTTT CGTCTCCAAG TACAAGGACC TTGTCTTCTG 
GCAGGAATAG AAAGTACTTC TCATGCTCTC CGTGCTGATG CTAGTCCATT TGCTGTGTCA 
GCCTCTTCTG TTTGGATCAG TTTGGCAGAA GAACATGACT GTAACCGAGC TTTGGAAATA 
ATCCTGCATC CTAGTGAACC ATACATTCCT CACTTATGTT TGGAGAGAGG CAAAAAAAGT 
TTCTCTCAAT ACAAAATGCA GATAAGAGAG AGAGGAGACT TCATAAGGGC AGTTAAGAAA 
TCTAGTGACC CAGAAAGACA GGTCAGCTTT GTAAGGAGAC GCTTCCACAA AGATCTCCAT 
TTTACCCCAG TCTTGATGTT GAACTTCTGC CCTGACCTTA CTGCCCTCAG CTCATTAATC 
TATGACCTCC CTACTATAAC CAGAGAAGTC ATTTTCCTTG TTTATCACTC AGGGGAGCTT 
TCGCATAATG TCCGGGATGC TCTCTTGCTG GCTGTTAGGA GTTTACCTGC ACGTACTTTG 
CTGAATCTGG GAAGTGGCAC ATGTGACAGC ATAACCCTGT TTCCGACCAG CAGGCAGTGT 
AGCAATGAGT CATTGGAGGC AGCTTATGAT CATATTAAGA ATGGACAACT CAGTTGCAGT 
TCAAGTAACT TGGTGACTGT TCTGGGCCAT ATCCTGTCTC TTCCAGTGCA TCGTGGATAC 
CCACGGCAGC TCTTTCTCTT CACTGACACA TCTCCAAGGT GTCCTGAAAG AGCCATTGAG 
CTTTTGTGTC ACCATTCAAA GACTACAAGG GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT 
GGCCATATAT TTCTTGAAGA AGCAGCCAGA GTGAGTAGTG GTACGGTGGA GCTGCTAAGT 
GAGGGGGAAA GACTGCATCC CAAGGTGATT AAATCATTAA AGAAGGCGCT GGAGCCAGCT 
CTGAGCGATA TCACTATAGA TTGGTACATG CCAGATACTA TGGAGGCATT GCTGACACCT 
AATGAAATTG CTCCACTTTA CCATGGGGAC TCTCTTATTA GTTATTGTGC TTTGTATCAC 
ACAGCCAGCT TCCGTGGCAA GAGGGACATG AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC 
TCAGTCTTTG AGTCCCAGGA AGATTCCCAG GCCCCAGGGA GCACAGACAC AAGCTGTCTA 
AATCCACCCT CAGGTGCTGA TGATATGGGT CGTGCCCTAC AGGAGATTTC CCAGGAAATA 
TCTTTGGAGT TTTCAGCATG CAGTGAGAAG CCAGATATAA GTGAAGATTC TTTCAGTGAC 
ATCAGGAAAC GCATTGTGCG TTCATCATAT GTAAAGGATC AGTATGTCCT CACCCATTGC 
TCTATCAGTA CTGAGCCAAC TCATGTCCAG ACCCACCCCT CCATCAGCAG TGATCCCACT 
AATACCCCTA CTCGTGGCTC TACTAGCAGT GAATCTGTGG GATCCCATGA TGCTAATGGA 
CACTGCATTT CAGCCGTGAC CTCACAACAA GGACAGAAGA CTGTGTCCGA GGTGGCAGGA 
ATTCATCCTA TCCGAGATCT TGCGTCTTCC AGTAAGGCAC CAGGAACCCA GAGCTCTGAA 
GAACTAATCA AAAGGAAGGC ACTGATGCAG GCCACCATGT CTGGTCGCAG TTTCTCGTCT 
CCCCATGGGG AACTGGATAT GCTGCGGCTG CGTTCTGTCT TGGACAAGGT TTCTCAAAGC 
AGGGAGCCAG ATGGGCGCTG CGCACGTGGA GAAAGCTTAA TGGATTCCTG TCATCTCCTT 
ACCCCTTCCC AGTTGGACTG GGACATGTTG GTTGATGCTC AGTATCTGTT TTCTGCTTCT 
CCTGCTGCAG AAGAAAACAG TGTGATATCT AAAGCAAGCA CTGACACTGT ATGGCAGTGC 
AGGGCTGTGA TACGTGGCCT TTTAGGAAAC AAGAGCCACT CTTGGGAGGC CTCAATAAGT 
CTGCTCTCAC TTATGCACTC TAATGAAGAG GAATCGAATG GTCCCTATGT GGTAGATTCT 
GAATTTGATT TGCATCATCT GGCTGCACAG TCTGTCATTA AAGATAATGA GGATACAGCA 
TGGAGAGTGT GTGACATTGA ACATGGGTCT TCTCGTAGAT ATCGCCTGAA AGCCATACAG 
ACCAGCAAAT CATGCTCTCA AATTTCCACA TTTACTAACC ATGTGTCTGT AGATCTTGCT 
ACCCATGATG TTGTTTCGGA GCACATAAAA GTATGTAGAG GTTGGCAAAA TAATAGAACC 
CACAGCACAA ACCTGGCTAA TTTGCATGAA GGAGAAAACA GAAGCTCAGA AAACATAGAT 
GAAGTCCTCA TGGCTTCAGA TGACACTGCA GATTTCCCAT CATCCCCACA GAGCCTAATC 
TCCACCAGTG GTTCGGTAAA ACAGCGTAGT CAGGAAGGTC AAATTCTCCA TGCCTTTGGC 
TCTCAGAAAC CATCAGAATC ATCGCTGAGT TCCAGGATCA GCAATGGAAA AAGAAAAATC 
CGAAGTATTT CTGGAAAACT TTCGCCCCTG AAACCATTAT GCCTAAGTGA GAGGGAGAAG 
GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC TATCTGCCAA TGATTAGCTT GCAGTCCCCA 
GAAGGATACT TCACACTAAA CAGCAATTTC TCCAGTATGG TTAAAATTTC TCTTGAGCGC 
CTGCTGCGTG CCTCACCCTA TAGCTGCCAC CGTGGTAGCT TAAGTCCAGT GTCTGAACCA 
AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA GGAGGAATGG GAACCTACAA GGGGGGTTCC 
CAACCACAGG TTCCCACTGC ACAAGGTCCA TCCTTTATTC CTGCACATGC TACATCATGC 
CCTGAGGCAC TGTGTCAGCA GGCAGACAGT GGACGTGGAT CTGAAACAGA CCCATGTGAA 
ACATCCCCAT GCCCTTCAGA GGTTTGCACT GATATGGACA CTGACCTAGA GGGTTGCTCC 
TGGGCCACTG CCGTGGCATT AGCATGGCTA GAACATTGCT CTGCAGGATT TTTTACTGAA 
TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA TGGTTAAGAG CACAGGTTTT GCCAGATGGG 
CTACAGCTCT CTGCATTAAA AGGAGCTGCC CGCCAGCTTT TCATCTTGCT CCGTCATTGG 
GATGAGAATC TCAACCTGAA TATGCTGTGC TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818822.1
CDS	620..4510
Translation	MPGLYNALSWVQLPLESSSVTAYANGYCMGLNAHLTYLNPGNETVQAVFIYLLDDGEVVTSFEAVTGNQKVQAQVQSQSQMEECCTKFPWDFLPCFCPNGHLILDSNSAHSVFILSVGEVPPSAALSVYLRSSRELKTLPNGAVRVILNSTLTPFAPSPGVPSEEGECLCDDRLCLCPTSCFGGVSGAGGNPWALLSESPPLVLPLPSAGSLAGALAEPATNAQRYQFSFRLQVQGPCLLAGIESTSHALRADASPFAVSASSVWISLAEEHDCNRALEIILHPSEPYIPHLCLERGKKSFSQYKMQIRERGDFIRAVKKSSDPERQVSFVRRRFHKDLHFTPVLMLNFCPDLTALSSLIYDLPTITREVIFLVYHSGELSHNVRDALLLAVRSLPARTLLNLGSGTCDSITLFPTSRQCSNESLEAAYDHIKNGQLSCSSSNLVTVLGHILSLPVHRGYPRQLFLFTDTSPRCPERAIELLCHHSKTTRVFGFGLGVKAGHIFLEEAARVSSGTVELLSEGERLHPKVIKSLKKALEPALSDITIDWYMPDTMEALLTPNEIAPLYHGDSLISYCALYHTASFRGKRDMRKSARCGSFSSVFESQEDSQAPGSTDTSCLNPPSGADDMGRALQEISQEISLEFSACSEKPDISEDSFSDIRKRIVRSSYVKDQYVLTHCSISTEPTHVQTHPSISSDPTNTPTRGSTSSESVGSHDANGHCISAVTSQQGQKTVSEVAGIHPIRDLASSSKAPGTQSSEELIKRKALMQATMSGRSFSSPHGELDMLRLRSVLDKVSQSREPDGRCARGESLMDSCHLLTPSQLDWDMLVDAQYLFSASPAAEENSVISKASTDTVWQCRAVIRGLLGNKSHSWEASISLLSLMHSNEEESNGPYVVDSEFDLHHLAAQSVIKDNEDTAWRVCDIEHGSSRRYRLKAIQTSKSCSQISTFTNHVSVDLATHDVVSEHIKVCRGWQNNRTHSTNLANLHEGENRSSENIDEVLMASDDTADFPSSPQSLISTSGSVKQRSQEGQILHAFGSQKPSESSLSSRISNGKRKIRSISGKLSPLKPLCLSEREKERSEESGFQDYLPMISLQSPEGYFTLNSNFSSMVKISLERLLRASPYSCHRGSLSPVSEPSLLEQGHFLKGGMGTYKGGSQPQVPTAQGPSFIPAHATSCPEALCQQADSGRGSETDPCETSPCPSEVCTDMDTDLEGCSWATAVALAWLEHCSAGFFTEWEVLAAKAEVWLRAQVLPDGLQLSALKGAARQLFILLRHWDENLNLNMLCYKPGNV

Target ORF information:

RefSeq Version	XM_012963368.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963368.2

ORF Insert Sequence:

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGGT TGTGCTTATG TCCAACCAGC 
TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA AATCCATGGG CGTTGCTCAG TGAATCCCCT 
CCTCTGGTCC TGCCTTTACC CTCTGCAGGT TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT 
ACCAATGCAC AGCGTTATCA ATTCAGCTTT CGTCTCCAAG TACAAGGACC TTGTCTTCTG 
GCAGGAATAG AAAGTACTTC TCATGCTCTC CGTGCTGATG CTAGTCCATT TGCTGTGTCA 
GCCTCTTCTG TTTGGATCAG TTTGGCAGAA GAACATGACT GTAACCGAGC TTTGGAAATA 
ATCCTGCATC CTAGTGAACC ATACATTCCT CACTTATGTT TGGAGAGAGG CAAAAAAAGT 
TTCTCTCAAT ACAAAATGCA GATAAGAGAG AGAGGAGACT TCATAAGGGC AGTTAAGAAA 
TCTAGTGACC CAGAAAGACA GGTCAGCTTT GTAAGGAGAC GCTTCCACAA AGATCTCCAT 
TTTACCCCAG TCTTGATGTT GAACTTCTGC CCTGACCTTA CTGCCCTCAG CTCATTAATC 
TATGACCTCC CTACTATAAC CAGAGAAGTC ATTTTCCTTG TTTATCACTC AGGGGAGCTT 
TCGCATAATG TCCGGGATGC TCTCTTGCTG GCTGTTAGGA GTTTACCTGC ACGTACTTTG 
CTGAATCTGG GAAGTGGCAC ATGTGACAGC ATAACCCTGT TTCCGACCAG CAGGCAGTGT 
AGCAATGAGT CATTGGAGGC AGCTTATGAT CATATTAAGA ATGGACAACT CAGTTGCAGT 
TCAAGTAACT TGGTGACTGT TCTGGGCCAT ATCCTGTCTC TTCCAGTGCA TCGTGGATAC 
CCACGGCAGC TCTTTCTCTT CACTGACACA TCTCCAAGGT GTCCTGAAAG AGCCATTGAG 
CTTTTGTGTC ACCATTCAAA GACTACAAGG GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT 
GGCCATATAT TTCTTGAAGA AGCAGCCAGA GTGAGTAGTG GTACGGTGGA GCTGCTAAGT 
GAGGGGGAAA GACTGCATCC CAAGGTGATT AAATCATTAA AGAAGGCGCT GGAGCCAGCT 
CTGAGCGATA TCACTATAGA TTGGTACATG CCAGATACTA TGGAGGCATT GCTGACACCT 
AATGAAATTG CTCCACTTTA CCATGGGGAC TCTCTTATTA GTTATTGTGC TTTGTATCAC 
ACAGCCAGCT TCCGTGGCAA GAGGGACATG AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC 
TCAGTCTTTG AGTCCCAGGA AGATTCCCAG GCCCCAGGGA GCACAGACAC AAGCTGTCTA 
AATCCACCCT CAGGTGCTGA TGATATGGGT CGTGCCCTAC AGGAGATTTC CCAGGAAATA 
TCTTTGGAGT TTTCAGCATG CAGTGAGAAG CCAGATATAA GTGAAGATTC TTTCAGTGAC 
ATCAGGAAAC GCATTGTGCG TTCATCATAT GTAAAGGATC AGTATGTCCT CACCCATTGC 
TCTATCAGTA CTGAGCCAAC TCATGTCCAG ACCCACCCCT CCATCAGCAG TGATCCCACT 
AATACCCCTA CTCGTGGCTC TACTAGCAGT GAATCTGTGG GATCCCATGA TGCTAATGGA 
CACTGCATTT CAGCCGTGAC CTCACAACAA GGACAGAAGA CTGTGTCCGA GGTGGCAGGA 
ATTCATCCTA TCCGAGATCT TGCGTCTTCC AGTAAGGCAC CAGGAACCCA GAGCTCTGAA 
GAACTAATCA AAAGGAAGGC ACTGATGCAG GCCACCATGT CTGGTCGCAG TTTCTCGTCT 
CCCCATGGGG AACTGGATAT GCTGCGGCTG CGTTCTGTCT TGGACAAGGT TTCTCAAAGC 
AGGGAGCCAG ATGGGCGCTG CGCACGTGGA GAAAGCTTAA TGGATTCCTG TCATCTCCTT 
ACCCCTTCCC AGTTGGACTG GGACATGTTG GTTGATGCTC AGTATCTGTT TTCTGCTTCT 
CCTGCTGCAG AAGAAAACAG TGTGATATCT AAAGCAAGCA CTGACACTGT ATGGCAGTGC 
AGGGCTGTGA TACGTGGCCT TTTAGGAAAC AAGAGCCACT CTTGGGAGGC CTCAATAAGT 
CTGCTCTCAC TTATGCACTC TAATGAAGAG GAATCGAATG GTCCCTATGT GGTAGATTCT 
GAATTTGATT TGCATCATCT GGCTGCACAG TCTGTCATTA AAGATAATGA GGATACAGCA 
TGGAGAGTGT GTGACATTGA ACATGGGTCT TCTCGTAGAT ATCGCCTGAA AGCCATACAG 
ACCAGCAAAT CATGCTCTCA AATTTCCACA TTTACTAACC ATGTGTCTGT AGATCTTGCT 
ACCCATGATG TTGTTTCGGA GCACATAAAA GTATGTAGAG GTTGGCAAAA TAATAGAACC 
CACAGCACAA ACCTGGCTAA TTTGCATGAA GGAGAAAACA GAAGCTCAGA AAACATAGAT 
GAAGTCCTCA TGGCTTCAGA TGACACTGCA GATTTCCCAT CATCCCCACA GAGCCTAATC 
TCCACCAGTG GTTCGGTAAA ACAGCGTAGT CAGGAAGGTC AAATTCTCCA TGCCTTTGGC 
TCTCAGAAAC CATCAGAATC ATCGCTGAGT TCCAGGATCA GCAATGGAAA AAGAAAAATC 
CGAAGTATTT CTGGAAAACT TTCGCCCCTG AAACCATTAT GCCTAAGTGA GAGGGAGAAG 
GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC TATCTGCCAA TGATTAGCTT GCAGTCCCCA 
GAAGGATACT TCACACTAAA CAGCAATTTC TCCAGTATGG TTAAAATTTC TCTTGAGCGC 
CTGCTGCGTG CCTCACCCTA TAGCTGCCAC CGTGGTAGCT TAAGTCCAGT GTCTGAACCA 
AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA GGAGGAATGG GAACCTACAA GGGGGGTTCC 
CAACCACAGG TTCCCACTGC ACAAGGTCCA TCCTTTATTC CTGCACATGC TACATCATGC 
CCTGAGGCAC TGTGTCAGCA GGCAGACAGT GGACGTGGAT CTGAAACAGA CCCATGTGAA 
ACATCCCCAT GCCCTTCAGA GGTTTGCACT GATATGGACA CTGACCTAGA GGGTTGCTCC 
TGGGCCACTG CCGTGGCATT AGCATGGCTA GAACATTGCT CTGCAGGATT TTTTACTGAA 
TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA TGGTTAAGAG CACAGGTTTT GCCAGATGGG 
CTACAGCTCT CTGCATTAAA AGGAGCTGCC CGCCAGCTTT TCATCTTGCT CCGTCATTGG 
GATGAGAATC TCAACCTGAA TATGCTGTGC TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa5b2 ( XM_012963369.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818823.1 Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X3, mRNA.

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CloneID	OXa34064
Clone ID Related Accession (Same CDS sequence)	XM_018094413.2 , XM_012963368.2 , XM_012963369.2 , XM_012963368.3 , XM_012963369.3 , XM_018094413.1
Accession Version	XM_012963369.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor A domain-containing protein 5B2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGGT TGTGCTTATG TCCAACCAGC 
TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA AATCCATGGG CGTTGCTCAG TGAATCCCCT 
CCTCTGGTCC TGCCTTTACC CTCTGCAGGT TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT 
ACCAATGCAC AGCGTTATCA ATTCAGCTTT CGTCTCCAAG TACAAGGACC TTGTCTTCTG 
GCAGGAATAG AAAGTACTTC TCATGCTCTC CGTGCTGATG CTAGTCCATT TGCTGTGTCA 
GCCTCTTCTG TTTGGATCAG TTTGGCAGAA GAACATGACT GTAACCGAGC TTTGGAAATA 
ATCCTGCATC CTAGTGAACC ATACATTCCT CACTTATGTT TGGAGAGAGG CAAAAAAAGT 
TTCTCTCAAT ACAAAATGCA GATAAGAGAG AGAGGAGACT TCATAAGGGC AGTTAAGAAA 
TCTAGTGACC CAGAAAGACA GGTCAGCTTT GTAAGGAGAC GCTTCCACAA AGATCTCCAT 
TTTACCCCAG TCTTGATGTT GAACTTCTGC CCTGACCTTA CTGCCCTCAG CTCATTAATC 
TATGACCTCC CTACTATAAC CAGAGAAGTC ATTTTCCTTG TTTATCACTC AGGGGAGCTT 
TCGCATAATG TCCGGGATGC TCTCTTGCTG GCTGTTAGGA GTTTACCTGC ACGTACTTTG 
CTGAATCTGG GAAGTGGCAC ATGTGACAGC ATAACCCTGT TTCCGACCAG CAGGCAGTGT 
AGCAATGAGT CATTGGAGGC AGCTTATGAT CATATTAAGA ATGGACAACT CAGTTGCAGT 
TCAAGTAACT TGGTGACTGT TCTGGGCCAT ATCCTGTCTC TTCCAGTGCA TCGTGGATAC 
CCACGGCAGC TCTTTCTCTT CACTGACACA TCTCCAAGGT GTCCTGAAAG AGCCATTGAG 
CTTTTGTGTC ACCATTCAAA GACTACAAGG GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT 
GGCCATATAT TTCTTGAAGA AGCAGCCAGA GTGAGTAGTG GTACGGTGGA GCTGCTAAGT 
GAGGGGGAAA GACTGCATCC CAAGGTGATT AAATCATTAA AGAAGGCGCT GGAGCCAGCT 
CTGAGCGATA TCACTATAGA TTGGTACATG CCAGATACTA TGGAGGCATT GCTGACACCT 
AATGAAATTG CTCCACTTTA CCATGGGGAC TCTCTTATTA GTTATTGTGC TTTGTATCAC 
ACAGCCAGCT TCCGTGGCAA GAGGGACATG AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC 
TCAGTCTTTG AGTCCCAGGA AGATTCCCAG GCCCCAGGGA GCACAGACAC AAGCTGTCTA 
AATCCACCCT CAGGTGCTGA TGATATGGGT CGTGCCCTAC AGGAGATTTC CCAGGAAATA 
TCTTTGGAGT TTTCAGCATG CAGTGAGAAG CCAGATATAA GTGAAGATTC TTTCAGTGAC 
ATCAGGAAAC GCATTGTGCG TTCATCATAT GTAAAGGATC AGTATGTCCT CACCCATTGC 
TCTATCAGTA CTGAGCCAAC TCATGTCCAG ACCCACCCCT CCATCAGCAG TGATCCCACT 
AATACCCCTA CTCGTGGCTC TACTAGCAGT GAATCTGTGG GATCCCATGA TGCTAATGGA 
CACTGCATTT CAGCCGTGAC CTCACAACAA GGACAGAAGA CTGTGTCCGA GGTGGCAGGA 
ATTCATCCTA TCCGAGATCT TGCGTCTTCC AGTAAGGCAC CAGGAACCCA GAGCTCTGAA 
GAACTAATCA AAAGGAAGGC ACTGATGCAG GCCACCATGT CTGGTCGCAG TTTCTCGTCT 
CCCCATGGGG AACTGGATAT GCTGCGGCTG CGTTCTGTCT TGGACAAGGT TTCTCAAAGC 
AGGGAGCCAG ATGGGCGCTG CGCACGTGGA GAAAGCTTAA TGGATTCCTG TCATCTCCTT 
ACCCCTTCCC AGTTGGACTG GGACATGTTG GTTGATGCTC AGTATCTGTT TTCTGCTTCT 
CCTGCTGCAG AAGAAAACAG TGTGATATCT AAAGCAAGCA CTGACACTGT ATGGCAGTGC 
AGGGCTGTGA TACGTGGCCT TTTAGGAAAC AAGAGCCACT CTTGGGAGGC CTCAATAAGT 
CTGCTCTCAC TTATGCACTC TAATGAAGAG GAATCGAATG GTCCCTATGT GGTAGATTCT 
GAATTTGATT TGCATCATCT GGCTGCACAG TCTGTCATTA AAGATAATGA GGATACAGCA 
TGGAGAGTGT GTGACATTGA ACATGGGTCT TCTCGTAGAT ATCGCCTGAA AGCCATACAG 
ACCAGCAAAT CATGCTCTCA AATTTCCACA TTTACTAACC ATGTGTCTGT AGATCTTGCT 
ACCCATGATG TTGTTTCGGA GCACATAAAA GTATGTAGAG GTTGGCAAAA TAATAGAACC 
CACAGCACAA ACCTGGCTAA TTTGCATGAA GGAGAAAACA GAAGCTCAGA AAACATAGAT 
GAAGTCCTCA TGGCTTCAGA TGACACTGCA GATTTCCCAT CATCCCCACA GAGCCTAATC 
TCCACCAGTG GTTCGGTAAA ACAGCGTAGT CAGGAAGGTC AAATTCTCCA TGCCTTTGGC 
TCTCAGAAAC CATCAGAATC ATCGCTGAGT TCCAGGATCA GCAATGGAAA AAGAAAAATC 
CGAAGTATTT CTGGAAAACT TTCGCCCCTG AAACCATTAT GCCTAAGTGA GAGGGAGAAG 
GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC TATCTGCCAA TGATTAGCTT GCAGTCCCCA 
GAAGGATACT TCACACTAAA CAGCAATTTC TCCAGTATGG TTAAAATTTC TCTTGAGCGC 
CTGCTGCGTG CCTCACCCTA TAGCTGCCAC CGTGGTAGCT TAAGTCCAGT GTCTGAACCA 
AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA GGAGGAATGG GAACCTACAA GGGGGGTTCC 
CAACCACAGG TTCCCACTGC ACAAGGTCCA TCCTTTATTC CTGCACATGC TACATCATGC 
CCTGAGGCAC TGTGTCAGCA GGCAGACAGT GGACGTGGAT CTGAAACAGA CCCATGTGAA 
ACATCCCCAT GCCCTTCAGA GGTTTGCACT GATATGGACA CTGACCTAGA GGGTTGCTCC 
TGGGCCACTG CCGTGGCATT AGCATGGCTA GAACATTGCT CTGCAGGATT TTTTACTGAA 
TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA TGGTTAAGAG CACAGGTTTT GCCAGATGGG 
CTACAGCTCT CTGCATTAAA AGGAGCTGCC CGCCAGCTTT TCATCTTGCT CCGTCATTGG 
GATGAGAATC TCAACCTGAA TATGCTGTGC TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818823.1
CDS	596..4486
Translation	MPGLYNALSWVQLPLESSSVTAYANGYCMGLNAHLTYLNPGNETVQAVFIYLLDDGEVVTSFEAVTGNQKVQAQVQSQSQMEECCTKFPWDFLPCFCPNGHLILDSNSAHSVFILSVGEVPPSAALSVYLRSSRELKTLPNGAVRVILNSTLTPFAPSPGVPSEEGECLCDDRLCLCPTSCFGGVSGAGGNPWALLSESPPLVLPLPSAGSLAGALAEPATNAQRYQFSFRLQVQGPCLLAGIESTSHALRADASPFAVSASSVWISLAEEHDCNRALEIILHPSEPYIPHLCLERGKKSFSQYKMQIRERGDFIRAVKKSSDPERQVSFVRRRFHKDLHFTPVLMLNFCPDLTALSSLIYDLPTITREVIFLVYHSGELSHNVRDALLLAVRSLPARTLLNLGSGTCDSITLFPTSRQCSNESLEAAYDHIKNGQLSCSSSNLVTVLGHILSLPVHRGYPRQLFLFTDTSPRCPERAIELLCHHSKTTRVFGFGLGVKAGHIFLEEAARVSSGTVELLSEGERLHPKVIKSLKKALEPALSDITIDWYMPDTMEALLTPNEIAPLYHGDSLISYCALYHTASFRGKRDMRKSARCGSFSSVFESQEDSQAPGSTDTSCLNPPSGADDMGRALQEISQEISLEFSACSEKPDISEDSFSDIRKRIVRSSYVKDQYVLTHCSISTEPTHVQTHPSISSDPTNTPTRGSTSSESVGSHDANGHCISAVTSQQGQKTVSEVAGIHPIRDLASSSKAPGTQSSEELIKRKALMQATMSGRSFSSPHGELDMLRLRSVLDKVSQSREPDGRCARGESLMDSCHLLTPSQLDWDMLVDAQYLFSASPAAEENSVISKASTDTVWQCRAVIRGLLGNKSHSWEASISLLSLMHSNEEESNGPYVVDSEFDLHHLAAQSVIKDNEDTAWRVCDIEHGSSRRYRLKAIQTSKSCSQISTFTNHVSVDLATHDVVSEHIKVCRGWQNNRTHSTNLANLHEGENRSSENIDEVLMASDDTADFPSSPQSLISTSGSVKQRSQEGQILHAFGSQKPSESSLSSRISNGKRKIRSISGKLSPLKPLCLSEREKERSEESGFQDYLPMISLQSPEGYFTLNSNFSSMVKISLERLLRASPYSCHRGSLSPVSEPSLLEQGHFLKGGMGTYKGGSQPQVPTAQGPSFIPAHATSCPEALCQQADSGRGSETDPCETSPCPSEVCTDMDTDLEGCSWATAVALAWLEHCSAGFFTEWEVLAAKAEVWLRAQVLPDGLQLSALKGAARQLFILLRHWDENLNLNMLCYKPGNV

Target ORF information:

RefSeq Version	XM_012963369.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963369.2

ORF Insert Sequence:

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGGT TGTGCTTATG TCCAACCAGC 
TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA AATCCATGGG CGTTGCTCAG TGAATCCCCT 
CCTCTGGTCC TGCCTTTACC CTCTGCAGGT TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT 
ACCAATGCAC AGCGTTATCA ATTCAGCTTT CGTCTCCAAG TACAAGGACC TTGTCTTCTG 
GCAGGAATAG AAAGTACTTC TCATGCTCTC CGTGCTGATG CTAGTCCATT TGCTGTGTCA 
GCCTCTTCTG TTTGGATCAG TTTGGCAGAA GAACATGACT GTAACCGAGC TTTGGAAATA 
ATCCTGCATC CTAGTGAACC ATACATTCCT CACTTATGTT TGGAGAGAGG CAAAAAAAGT 
TTCTCTCAAT ACAAAATGCA GATAAGAGAG AGAGGAGACT TCATAAGGGC AGTTAAGAAA 
TCTAGTGACC CAGAAAGACA GGTCAGCTTT GTAAGGAGAC GCTTCCACAA AGATCTCCAT 
TTTACCCCAG TCTTGATGTT GAACTTCTGC CCTGACCTTA CTGCCCTCAG CTCATTAATC 
TATGACCTCC CTACTATAAC CAGAGAAGTC ATTTTCCTTG TTTATCACTC AGGGGAGCTT 
TCGCATAATG TCCGGGATGC TCTCTTGCTG GCTGTTAGGA GTTTACCTGC ACGTACTTTG 
CTGAATCTGG GAAGTGGCAC ATGTGACAGC ATAACCCTGT TTCCGACCAG CAGGCAGTGT 
AGCAATGAGT CATTGGAGGC AGCTTATGAT CATATTAAGA ATGGACAACT CAGTTGCAGT 
TCAAGTAACT TGGTGACTGT TCTGGGCCAT ATCCTGTCTC TTCCAGTGCA TCGTGGATAC 
CCACGGCAGC TCTTTCTCTT CACTGACACA TCTCCAAGGT GTCCTGAAAG AGCCATTGAG 
CTTTTGTGTC ACCATTCAAA GACTACAAGG GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT 
GGCCATATAT TTCTTGAAGA AGCAGCCAGA GTGAGTAGTG GTACGGTGGA GCTGCTAAGT 
GAGGGGGAAA GACTGCATCC CAAGGTGATT AAATCATTAA AGAAGGCGCT GGAGCCAGCT 
CTGAGCGATA TCACTATAGA TTGGTACATG CCAGATACTA TGGAGGCATT GCTGACACCT 
AATGAAATTG CTCCACTTTA CCATGGGGAC TCTCTTATTA GTTATTGTGC TTTGTATCAC 
ACAGCCAGCT TCCGTGGCAA GAGGGACATG AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC 
TCAGTCTTTG AGTCCCAGGA AGATTCCCAG GCCCCAGGGA GCACAGACAC AAGCTGTCTA 
AATCCACCCT CAGGTGCTGA TGATATGGGT CGTGCCCTAC AGGAGATTTC CCAGGAAATA 
TCTTTGGAGT TTTCAGCATG CAGTGAGAAG CCAGATATAA GTGAAGATTC TTTCAGTGAC 
ATCAGGAAAC GCATTGTGCG TTCATCATAT GTAAAGGATC AGTATGTCCT CACCCATTGC 
TCTATCAGTA CTGAGCCAAC TCATGTCCAG ACCCACCCCT CCATCAGCAG TGATCCCACT 
AATACCCCTA CTCGTGGCTC TACTAGCAGT GAATCTGTGG GATCCCATGA TGCTAATGGA 
CACTGCATTT CAGCCGTGAC CTCACAACAA GGACAGAAGA CTGTGTCCGA GGTGGCAGGA 
ATTCATCCTA TCCGAGATCT TGCGTCTTCC AGTAAGGCAC CAGGAACCCA GAGCTCTGAA 
GAACTAATCA AAAGGAAGGC ACTGATGCAG GCCACCATGT CTGGTCGCAG TTTCTCGTCT 
CCCCATGGGG AACTGGATAT GCTGCGGCTG CGTTCTGTCT TGGACAAGGT TTCTCAAAGC 
AGGGAGCCAG ATGGGCGCTG CGCACGTGGA GAAAGCTTAA TGGATTCCTG TCATCTCCTT 
ACCCCTTCCC AGTTGGACTG GGACATGTTG GTTGATGCTC AGTATCTGTT TTCTGCTTCT 
CCTGCTGCAG AAGAAAACAG TGTGATATCT AAAGCAAGCA CTGACACTGT ATGGCAGTGC 
AGGGCTGTGA TACGTGGCCT TTTAGGAAAC AAGAGCCACT CTTGGGAGGC CTCAATAAGT 
CTGCTCTCAC TTATGCACTC TAATGAAGAG GAATCGAATG GTCCCTATGT GGTAGATTCT 
GAATTTGATT TGCATCATCT GGCTGCACAG TCTGTCATTA AAGATAATGA GGATACAGCA 
TGGAGAGTGT GTGACATTGA ACATGGGTCT TCTCGTAGAT ATCGCCTGAA AGCCATACAG 
ACCAGCAAAT CATGCTCTCA AATTTCCACA TTTACTAACC ATGTGTCTGT AGATCTTGCT 
ACCCATGATG TTGTTTCGGA GCACATAAAA GTATGTAGAG GTTGGCAAAA TAATAGAACC 
CACAGCACAA ACCTGGCTAA TTTGCATGAA GGAGAAAACA GAAGCTCAGA AAACATAGAT 
GAAGTCCTCA TGGCTTCAGA TGACACTGCA GATTTCCCAT CATCCCCACA GAGCCTAATC 
TCCACCAGTG GTTCGGTAAA ACAGCGTAGT CAGGAAGGTC AAATTCTCCA TGCCTTTGGC 
TCTCAGAAAC CATCAGAATC ATCGCTGAGT TCCAGGATCA GCAATGGAAA AAGAAAAATC 
CGAAGTATTT CTGGAAAACT TTCGCCCCTG AAACCATTAT GCCTAAGTGA GAGGGAGAAG 
GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC TATCTGCCAA TGATTAGCTT GCAGTCCCCA 
GAAGGATACT TCACACTAAA CAGCAATTTC TCCAGTATGG TTAAAATTTC TCTTGAGCGC 
CTGCTGCGTG CCTCACCCTA TAGCTGCCAC CGTGGTAGCT TAAGTCCAGT GTCTGAACCA 
AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA GGAGGAATGG GAACCTACAA GGGGGGTTCC 
CAACCACAGG TTCCCACTGC ACAAGGTCCA TCCTTTATTC CTGCACATGC TACATCATGC 
CCTGAGGCAC TGTGTCAGCA GGCAGACAGT GGACGTGGAT CTGAAACAGA CCCATGTGAA 
ACATCCCCAT GCCCTTCAGA GGTTTGCACT GATATGGACA CTGACCTAGA GGGTTGCTCC 
TGGGCCACTG CCGTGGCATT AGCATGGCTA GAACATTGCT CTGCAGGATT TTTTACTGAA 
TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA TGGTTAAGAG CACAGGTTTT GCCAGATGGG 
CTACAGCTCT CTGCATTAAA AGGAGCTGCC CGCCAGCTTT TCATCTTGCT CCGTCATTGG 
GATGAGAATC TCAACCTGAA TATGCTGTGC TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwa5b2 ( XM_018094413.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949902.1 Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X4, mRNA.

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CloneID	OXa34064
Clone ID Related Accession (Same CDS sequence)	XM_018094413.2 , XM_012963368.2 , XM_012963369.2 , XM_012963368.3 , XM_012963369.3 , XM_018094413.1
Accession Version	XM_018094413.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor A domain-containing protein 5B2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGGT TGTGCTTATG TCCAACCAGC 
TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA AATCCATGGG CGTTGCTCAG TGAATCCCCT 
CCTCTGGTCC TGCCTTTACC CTCTGCAGGT TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT 
ACCAATGCAC AGCGTTATCA ATTCAGCTTT CGTCTCCAAG TACAAGGACC TTGTCTTCTG 
GCAGGAATAG AAAGTACTTC TCATGCTCTC CGTGCTGATG CTAGTCCATT TGCTGTGTCA 
GCCTCTTCTG TTTGGATCAG TTTGGCAGAA GAACATGACT GTAACCGAGC TTTGGAAATA 
ATCCTGCATC CTAGTGAACC ATACATTCCT CACTTATGTT TGGAGAGAGG CAAAAAAAGT 
TTCTCTCAAT ACAAAATGCA GATAAGAGAG AGAGGAGACT TCATAAGGGC AGTTAAGAAA 
TCTAGTGACC CAGAAAGACA GGTCAGCTTT GTAAGGAGAC GCTTCCACAA AGATCTCCAT 
TTTACCCCAG TCTTGATGTT GAACTTCTGC CCTGACCTTA CTGCCCTCAG CTCATTAATC 
TATGACCTCC CTACTATAAC CAGAGAAGTC ATTTTCCTTG TTTATCACTC AGGGGAGCTT 
TCGCATAATG TCCGGGATGC TCTCTTGCTG GCTGTTAGGA GTTTACCTGC ACGTACTTTG 
CTGAATCTGG GAAGTGGCAC ATGTGACAGC ATAACCCTGT TTCCGACCAG CAGGCAGTGT 
AGCAATGAGT CATTGGAGGC AGCTTATGAT CATATTAAGA ATGGACAACT CAGTTGCAGT 
TCAAGTAACT TGGTGACTGT TCTGGGCCAT ATCCTGTCTC TTCCAGTGCA TCGTGGATAC 
CCACGGCAGC TCTTTCTCTT CACTGACACA TCTCCAAGGT GTCCTGAAAG AGCCATTGAG 
CTTTTGTGTC ACCATTCAAA GACTACAAGG GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT 
GGCCATATAT TTCTTGAAGA AGCAGCCAGA GTGAGTAGTG GTACGGTGGA GCTGCTAAGT 
GAGGGGGAAA GACTGCATCC CAAGGTGATT AAATCATTAA AGAAGGCGCT GGAGCCAGCT 
CTGAGCGATA TCACTATAGA TTGGTACATG CCAGATACTA TGGAGGCATT GCTGACACCT 
AATGAAATTG CTCCACTTTA CCATGGGGAC TCTCTTATTA GTTATTGTGC TTTGTATCAC 
ACAGCCAGCT TCCGTGGCAA GAGGGACATG AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC 
TCAGTCTTTG AGTCCCAGGA AGATTCCCAG GCCCCAGGGA GCACAGACAC AAGCTGTCTA 
AATCCACCCT CAGGTGCTGA TGATATGGGT CGTGCCCTAC AGGAGATTTC CCAGGAAATA 
TCTTTGGAGT TTTCAGCATG CAGTGAGAAG CCAGATATAA GTGAAGATTC TTTCAGTGAC 
ATCAGGAAAC GCATTGTGCG TTCATCATAT GTAAAGGATC AGTATGTCCT CACCCATTGC 
TCTATCAGTA CTGAGCCAAC TCATGTCCAG ACCCACCCCT CCATCAGCAG TGATCCCACT 
AATACCCCTA CTCGTGGCTC TACTAGCAGT GAATCTGTGG GATCCCATGA TGCTAATGGA 
CACTGCATTT CAGCCGTGAC CTCACAACAA GGACAGAAGA CTGTGTCCGA GGTGGCAGGA 
ATTCATCCTA TCCGAGATCT TGCGTCTTCC AGTAAGGCAC CAGGAACCCA GAGCTCTGAA 
GAACTAATCA AAAGGAAGGC ACTGATGCAG GCCACCATGT CTGGTCGCAG TTTCTCGTCT 
CCCCATGGGG AACTGGATAT GCTGCGGCTG CGTTCTGTCT TGGACAAGGT TTCTCAAAGC 
AGGGAGCCAG ATGGGCGCTG CGCACGTGGA GAAAGCTTAA TGGATTCCTG TCATCTCCTT 
ACCCCTTCCC AGTTGGACTG GGACATGTTG GTTGATGCTC AGTATCTGTT TTCTGCTTCT 
CCTGCTGCAG AAGAAAACAG TGTGATATCT AAAGCAAGCA CTGACACTGT ATGGCAGTGC 
AGGGCTGTGA TACGTGGCCT TTTAGGAAAC AAGAGCCACT CTTGGGAGGC CTCAATAAGT 
CTGCTCTCAC TTATGCACTC TAATGAAGAG GAATCGAATG GTCCCTATGT GGTAGATTCT 
GAATTTGATT TGCATCATCT GGCTGCACAG TCTGTCATTA AAGATAATGA GGATACAGCA 
TGGAGAGTGT GTGACATTGA ACATGGGTCT TCTCGTAGAT ATCGCCTGAA AGCCATACAG 
ACCAGCAAAT CATGCTCTCA AATTTCCACA TTTACTAACC ATGTGTCTGT AGATCTTGCT 
ACCCATGATG TTGTTTCGGA GCACATAAAA GTATGTAGAG GTTGGCAAAA TAATAGAACC 
CACAGCACAA ACCTGGCTAA TTTGCATGAA GGAGAAAACA GAAGCTCAGA AAACATAGAT 
GAAGTCCTCA TGGCTTCAGA TGACACTGCA GATTTCCCAT CATCCCCACA GAGCCTAATC 
TCCACCAGTG GTTCGGTAAA ACAGCGTAGT CAGGAAGGTC AAATTCTCCA TGCCTTTGGC 
TCTCAGAAAC CATCAGAATC ATCGCTGAGT TCCAGGATCA GCAATGGAAA AAGAAAAATC 
CGAAGTATTT CTGGAAAACT TTCGCCCCTG AAACCATTAT GCCTAAGTGA GAGGGAGAAG 
GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC TATCTGCCAA TGATTAGCTT GCAGTCCCCA 
GAAGGATACT TCACACTAAA CAGCAATTTC TCCAGTATGG TTAAAATTTC TCTTGAGCGC 
CTGCTGCGTG CCTCACCCTA TAGCTGCCAC CGTGGTAGCT TAAGTCCAGT GTCTGAACCA 
AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA GGAGGAATGG GAACCTACAA GGGGGGTTCC 
CAACCACAGG TTCCCACTGC ACAAGGTCCA TCCTTTATTC CTGCACATGC TACATCATGC 
CCTGAGGCAC TGTGTCAGCA GGCAGACAGT GGACGTGGAT CTGAAACAGA CCCATGTGAA 
ACATCCCCAT GCCCTTCAGA GGTTTGCACT GATATGGACA CTGACCTAGA GGGTTGCTCC 
TGGGCCACTG CCGTGGCATT AGCATGGCTA GAACATTGCT CTGCAGGATT TTTTACTGAA 
TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA TGGTTAAGAG CACAGGTTTT GCCAGATGGG 
CTACAGCTCT CTGCATTAAA AGGAGCTGCC CGCCAGCTTT TCATCTTGCT CCGTCATTGG 
GATGAGAATC TCAACCTGAA TATGCTGTGC TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949902.1
CDS	549..4439
Translation	MPGLYNALSWVQLPLESSSVTAYANGYCMGLNAHLTYLNPGNETVQAVFIYLLDDGEVVTSFEAVTGNQKVQAQVQSQSQMEECCTKFPWDFLPCFCPNGHLILDSNSAHSVFILSVGEVPPSAALSVYLRSSRELKTLPNGAVRVILNSTLTPFAPSPGVPSEEGECLCDDRLCLCPTSCFGGVSGAGGNPWALLSESPPLVLPLPSAGSLAGALAEPATNAQRYQFSFRLQVQGPCLLAGIESTSHALRADASPFAVSASSVWISLAEEHDCNRALEIILHPSEPYIPHLCLERGKKSFSQYKMQIRERGDFIRAVKKSSDPERQVSFVRRRFHKDLHFTPVLMLNFCPDLTALSSLIYDLPTITREVIFLVYHSGELSHNVRDALLLAVRSLPARTLLNLGSGTCDSITLFPTSRQCSNESLEAAYDHIKNGQLSCSSSNLVTVLGHILSLPVHRGYPRQLFLFTDTSPRCPERAIELLCHHSKTTRVFGFGLGVKAGHIFLEEAARVSSGTVELLSEGERLHPKVIKSLKKALEPALSDITIDWYMPDTMEALLTPNEIAPLYHGDSLISYCALYHTASFRGKRDMRKSARCGSFSSVFESQEDSQAPGSTDTSCLNPPSGADDMGRALQEISQEISLEFSACSEKPDISEDSFSDIRKRIVRSSYVKDQYVLTHCSISTEPTHVQTHPSISSDPTNTPTRGSTSSESVGSHDANGHCISAVTSQQGQKTVSEVAGIHPIRDLASSSKAPGTQSSEELIKRKALMQATMSGRSFSSPHGELDMLRLRSVLDKVSQSREPDGRCARGESLMDSCHLLTPSQLDWDMLVDAQYLFSASPAAEENSVISKASTDTVWQCRAVIRGLLGNKSHSWEASISLLSLMHSNEEESNGPYVVDSEFDLHHLAAQSVIKDNEDTAWRVCDIEHGSSRRYRLKAIQTSKSCSQISTFTNHVSVDLATHDVVSEHIKVCRGWQNNRTHSTNLANLHEGENRSSENIDEVLMASDDTADFPSSPQSLISTSGSVKQRSQEGQILHAFGSQKPSESSLSSRISNGKRKIRSISGKLSPLKPLCLSEREKERSEESGFQDYLPMISLQSPEGYFTLNSNFSSMVKISLERLLRASPYSCHRGSLSPVSEPSLLEQGHFLKGGMGTYKGGSQPQVPTAQGPSFIPAHATSCPEALCQQADSGRGSETDPCETSPCPSEVCTDMDTDLEGCSWATAVALAWLEHCSAGFFTEWEVLAAKAEVWLRAQVLPDGLQLSALKGAARQLFILLRHWDENLNLNMLCYKPGNV

Target ORF information:

RefSeq Version	XM_018094413.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor A domain containing 5B2 (vwa5b2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094413.1

ORF Insert Sequence:

ATGCCAGGTC TTTATAATGC CTTATCATGG GTTCAACTCC CTCTCGAGTC GTCCTCTGTA 
ACTGCCTATG CCAATGGATA CTGCATGGGT CTCAATGCCC ACCTCACATA TCTTAACCCA 
GGAAATGAAA CTGTTCAAGC TGTTTTTATA TATCTGCTGG ATGATGGAGA GGTGGTAACT 
AGCTTTGAGG CTGTGACTGG CAACCAAAAA GTACAAGCCC AGGTCCAAAG TCAAAGCCAA 
ATGGAGGAAT GCTGCACCAA GTTCCCTTGG GATTTTCTGC CTTGTTTTTG TCCCAATGGT 
CACCTTATCC TGGATTCAAA TAGTGCACAT AGTGTGTTTA TTCTCAGTGT TGGAGAAGTT 
CCTCCTTCTG CAGCACTAAG TGTGTACCTT AGGAGCAGCA GAGAGCTAAA GACTCTTCCA 
AATGGAGCAG TGCGTGTGAT CCTCAACTCC ACACTCACAC CCTTTGCCCC TTCTCCAGGG 
GTACCCAGTG AAGAGGGGGA GTGTCTGTGT GACGACAGGT TGTGCTTATG TCCAACCAGC 
TGTTTTGGTG GTGTAAGTGG AGCAGGAGGA AATCCATGGG CGTTGCTCAG TGAATCCCCT 
CCTCTGGTCC TGCCTTTACC CTCTGCAGGT TCTCTTGCTG GTGCTCTAGC TGAGCCAGCT 
ACCAATGCAC AGCGTTATCA ATTCAGCTTT CGTCTCCAAG TACAAGGACC TTGTCTTCTG 
GCAGGAATAG AAAGTACTTC TCATGCTCTC CGTGCTGATG CTAGTCCATT TGCTGTGTCA 
GCCTCTTCTG TTTGGATCAG TTTGGCAGAA GAACATGACT GTAACCGAGC TTTGGAAATA 
ATCCTGCATC CTAGTGAACC ATACATTCCT CACTTATGTT TGGAGAGAGG CAAAAAAAGT 
TTCTCTCAAT ACAAAATGCA GATAAGAGAG AGAGGAGACT TCATAAGGGC AGTTAAGAAA 
TCTAGTGACC CAGAAAGACA GGTCAGCTTT GTAAGGAGAC GCTTCCACAA AGATCTCCAT 
TTTACCCCAG TCTTGATGTT GAACTTCTGC CCTGACCTTA CTGCCCTCAG CTCATTAATC 
TATGACCTCC CTACTATAAC CAGAGAAGTC ATTTTCCTTG TTTATCACTC AGGGGAGCTT 
TCGCATAATG TCCGGGATGC TCTCTTGCTG GCTGTTAGGA GTTTACCTGC ACGTACTTTG 
CTGAATCTGG GAAGTGGCAC ATGTGACAGC ATAACCCTGT TTCCGACCAG CAGGCAGTGT 
AGCAATGAGT CATTGGAGGC AGCTTATGAT CATATTAAGA ATGGACAACT CAGTTGCAGT 
TCAAGTAACT TGGTGACTGT TCTGGGCCAT ATCCTGTCTC TTCCAGTGCA TCGTGGATAC 
CCACGGCAGC TCTTTCTCTT CACTGACACA TCTCCAAGGT GTCCTGAAAG AGCCATTGAG 
CTTTTGTGTC ACCATTCAAA GACTACAAGG GTGTTTGGTT TTGGTCTTGG TGTCAAAGCT 
GGCCATATAT TTCTTGAAGA AGCAGCCAGA GTGAGTAGTG GTACGGTGGA GCTGCTAAGT 
GAGGGGGAAA GACTGCATCC CAAGGTGATT AAATCATTAA AGAAGGCGCT GGAGCCAGCT 
CTGAGCGATA TCACTATAGA TTGGTACATG CCAGATACTA TGGAGGCATT GCTGACACCT 
AATGAAATTG CTCCACTTTA CCATGGGGAC TCTCTTATTA GTTATTGTGC TTTGTATCAC 
ACAGCCAGCT TCCGTGGCAA GAGGGACATG AGAAAGAGTG CCAGGTGTGG CTCTTTTAGC 
TCAGTCTTTG AGTCCCAGGA AGATTCCCAG GCCCCAGGGA GCACAGACAC AAGCTGTCTA 
AATCCACCCT CAGGTGCTGA TGATATGGGT CGTGCCCTAC AGGAGATTTC CCAGGAAATA 
TCTTTGGAGT TTTCAGCATG CAGTGAGAAG CCAGATATAA GTGAAGATTC TTTCAGTGAC 
ATCAGGAAAC GCATTGTGCG TTCATCATAT GTAAAGGATC AGTATGTCCT CACCCATTGC 
TCTATCAGTA CTGAGCCAAC TCATGTCCAG ACCCACCCCT CCATCAGCAG TGATCCCACT 
AATACCCCTA CTCGTGGCTC TACTAGCAGT GAATCTGTGG GATCCCATGA TGCTAATGGA 
CACTGCATTT CAGCCGTGAC CTCACAACAA GGACAGAAGA CTGTGTCCGA GGTGGCAGGA 
ATTCATCCTA TCCGAGATCT TGCGTCTTCC AGTAAGGCAC CAGGAACCCA GAGCTCTGAA 
GAACTAATCA AAAGGAAGGC ACTGATGCAG GCCACCATGT CTGGTCGCAG TTTCTCGTCT 
CCCCATGGGG AACTGGATAT GCTGCGGCTG CGTTCTGTCT TGGACAAGGT TTCTCAAAGC 
AGGGAGCCAG ATGGGCGCTG CGCACGTGGA GAAAGCTTAA TGGATTCCTG TCATCTCCTT 
ACCCCTTCCC AGTTGGACTG GGACATGTTG GTTGATGCTC AGTATCTGTT TTCTGCTTCT 
CCTGCTGCAG AAGAAAACAG TGTGATATCT AAAGCAAGCA CTGACACTGT ATGGCAGTGC 
AGGGCTGTGA TACGTGGCCT TTTAGGAAAC AAGAGCCACT CTTGGGAGGC CTCAATAAGT 
CTGCTCTCAC TTATGCACTC TAATGAAGAG GAATCGAATG GTCCCTATGT GGTAGATTCT 
GAATTTGATT TGCATCATCT GGCTGCACAG TCTGTCATTA AAGATAATGA GGATACAGCA 
TGGAGAGTGT GTGACATTGA ACATGGGTCT TCTCGTAGAT ATCGCCTGAA AGCCATACAG 
ACCAGCAAAT CATGCTCTCA AATTTCCACA TTTACTAACC ATGTGTCTGT AGATCTTGCT 
ACCCATGATG TTGTTTCGGA GCACATAAAA GTATGTAGAG GTTGGCAAAA TAATAGAACC 
CACAGCACAA ACCTGGCTAA TTTGCATGAA GGAGAAAACA GAAGCTCAGA AAACATAGAT 
GAAGTCCTCA TGGCTTCAGA TGACACTGCA GATTTCCCAT CATCCCCACA GAGCCTAATC 
TCCACCAGTG GTTCGGTAAA ACAGCGTAGT CAGGAAGGTC AAATTCTCCA TGCCTTTGGC 
TCTCAGAAAC CATCAGAATC ATCGCTGAGT TCCAGGATCA GCAATGGAAA AAGAAAAATC 
CGAAGTATTT CTGGAAAACT TTCGCCCCTG AAACCATTAT GCCTAAGTGA GAGGGAGAAG 
GAGAGAAGCG AAGAGTCTGG GTTTCAGGAC TATCTGCCAA TGATTAGCTT GCAGTCCCCA 
GAAGGATACT TCACACTAAA CAGCAATTTC TCCAGTATGG TTAAAATTTC TCTTGAGCGC 
CTGCTGCGTG CCTCACCCTA TAGCTGCCAC CGTGGTAGCT TAAGTCCAGT GTCTGAACCA 
AGCCTTCTAG AGCAGGGGCA CTTCCTTAAA GGAGGAATGG GAACCTACAA GGGGGGTTCC 
CAACCACAGG TTCCCACTGC ACAAGGTCCA TCCTTTATTC CTGCACATGC TACATCATGC 
CCTGAGGCAC TGTGTCAGCA GGCAGACAGT GGACGTGGAT CTGAAACAGA CCCATGTGAA 
ACATCCCCAT GCCCTTCAGA GGTTTGCACT GATATGGACA CTGACCTAGA GGGTTGCTCC 
TGGGCCACTG CCGTGGCATT AGCATGGCTA GAACATTGCT CTGCAGGATT TTTTACTGAA 
TGGGAGGTAC TAGCTGCAAA AGCAGAGGTA TGGTTAAGAG CACAGGTTTT GCCAGATGGG 
CTACAGCTCT CTGCATTAAA AGGAGCTGCC CGCCAGCTTT TCATCTTGCT CCGTCATTGG 
GATGAGAATC TCAACCTGAA TATGCTGTGC TACAAGCCAG GTAATGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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vwa5b2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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