menf.2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	menf.2
Entrez Gene ID	100485520
Full Name	mesendoderm nuclear factor, gene 2
Synonyms	xmenf
General protein information	Preferred Names mesendoderm nuclear factor, gene 2 Names uncharacterized protein menf.2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031906504.1	XP_031762364.1	LOW QUALITY PROTEIN: uncharacterized protein menf.2
XM_002944911.4	XP_002944957.2	uncharacterized protein menf.2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100485520	XP_002944957.2
Xenopus tropicalis (tropical clawed frog)	menf.1	XP_004919677.1
Danio rerio (zebrafish)	LOC101884159	XP_005157358.1
Xenopus tropicalis (tropical clawed frog)	LOC101733751	XP_004919678.1
Xenopus tropicalis (tropical clawed frog)	LOC101733813	XP_004919679.1

Gene Ontology

The following menf.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the menf.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa19978	XM_002944911.4 Latest version!	Xenopus tropicalis mesendoderm nuclear factor, gene 2 (menf.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
OXa63013	XM_031906504.1 Latest version!	Xenopus tropicalis mesendoderm nuclear factor, gene 2 (menf.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

menf.2 ( XM_002944911.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002944957.2 Xenopus tropicalis mesendoderm nuclear factor, gene 2 (menf.2), mRNA.

Price & Availability↑

CloneID	OXa19978
Clone ID Related Accession (Same CDS sequence)	XM_002944911.4
Accession Version	XM_002944911.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	uncharacterized protein menf.2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016684900.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002944911.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGATTCTA AGAAGACACC AAACCGCTAT CATCTTGCCT GCAAGTTTTG CCTGAAGGTT 
ACAGGCACCT TGCCTAATCA TCTGAGGAGA GCCTGCATGA AACATGCAGA TAACCAAGAA 
ATTGAATTTG TGATGCAAGA AGCAAGAGGA AAGATGAGAG CAATCCTGGA AGGGCTAAGT 
ATTGTTAATT ATGAAGACCT GGACTATGAG GATTCTGATC CTAAACAATT TTTTGTCCAG 
TTCCTGGAGA AAAATGGCTG CTATATCAGA GGGAAGCTAG AGTCAGCAAG CTGTGCCATG 
AAAGAGAGCA TCCTTCCATC AAATATGGAC AGAAACAAGG TGAACGTCAA TTGGCCAGCA 
GATGAAGTAG GGGAGACACA GGCCCCTTGT AAAGATCTGG ACCTGTCACA TAAGATGATA 
AGCCCCAGCC CCTTGAATGC TGAAAATGGG CAGAAACAGC AACCTACACT TCAGTCACTT 
CAGGAGAGCA TTGACTTCCT AATGTCACAA TTGGCCAAAA AGCATGAGAA GGTAAACTCC 
AGACCCTTGA ATGCTGAAGC AGTGCAGAAA AAGCGACCTG TTGTACAACA GGGCCTACCC 
ATCGAGTCAG ATGAGGAGGA CTATGTAAGC CTTGTGAATG AACAAGAGGT GGAGATGCAG 
GGACCTGAGC TTGACCTCCC CATCGAGTCA GATGAGGAGA ACTTCATAAG CCCTGTGAAT 
GAACAAGAGG TGGAGATGCA GGGACCTGAG CTTGACCTCC CTGTTGAGTC AGAGGAGAAC 
AATGCAGACC CCTTGCTTGA TGACAACGAT GATGATGATT ATGATGATGA CGACAAGGAA 
AATGAAGAGA TGGAGACACA CAATCTTGTT GAAGGGCTGG AACAGACCGA GACCAATTCA 
AACTACATTC TGCTTAGAAG AAGAATGATG GCTGCAGGCA TGTATAGAAA ACACTCTCTT 
GAATGTGGTG TTCTTGCTCG CTTCCGAAAA TATCTGCAGC AAGAATTACA AGTAACAAGA 
TGGTCACAAG AGGTGGCAAA TGTGGCCAGG TTTTTGTATT TTGTGGACCC CTCCAAGGCG 
TCCTTAAGCT TCCTGACCGA CACACAGAAG ACGTTGGATT TTTTTACGGC ACTTGAGAAA 
TTGGGAAACA CTCACGCCAC AATGTTTTCG TACCTCAAAC ATATCAAGAG GTTTGTGAGA 
TCCCAAACAG ACACTTTGTC CAGCGGATCC CATCAAAGTG AAGACCTTGC CGGAAAATGC 
AAACGCTATC TCGACATGAC CGCAACTCTA CAGCGAAGGC TGAGCAAGGG AATTACGGCC 
AGAGTTGTGG GGAAAAAGTT TGACTATCTG ACGTCTGCAA AAAAGGACCC ATCAGAGTGT 
CAACGGATCC TCGTAGTGGC CAGACCAATA TTTGACCAGA TAATAAAGAA GGCCAAGATG 
GGTGTATGTC TTTTGGAGAA GGAAAAGTCT TATGTCATCT ATTACTTGGA GGCACTCATT 
GTTTTAAAAT ACCTCCAGCG ACCAGGGGTT GTTCAAAATA TGACTGTAGA AGAATGGTGC 
AAGAGGATGC CATCTGATTG CAAAACCCGT ATGGTCATTG GTGTAAAAAG GCATAAGACA 
TCCACTCAGC AAGTCGCCTC TATACTGCTA GAAGAAGAAG AAGAAGAGTG GTTTGATGTT 
TATTATAAAA AAATACGGCC ATCATTTATC AGGAGTGGGA AATGCCCACA GACATTTTTC 
ATATCGTCAA CAGGGGAACC AATTCACAGT GTGACAAATG ACATCGCCCG GCTTCATCAT 
AAGTTTAAAT TGCGCCCTGT CACAAGTCAA GAGGCTAGAA GGACAATGGA GACATACATG 
GTCTCCCACT TTCAAACTGA TGCTCAGAGG AACATGTTTG CCAGGTTGCT TGGCCATTCA 
AATGTGACTG CCGGAAGGAT ATATGGCGAG AAGACCGTTG ACAATATGGT AGAAGCGGCA 
GAAATGATGA AGAGGGCGAT GCATGAATCC CAGCCATCAA CATCAAGGTG TCAAGAGGTG 
CCACAGAAGC CCCTGCAAGA AGAAACACCA GAAGTCTCCC GTGGGTTGAG GGAGGAAGCC 
TTGAAAAATT TCAAGCCTGC GAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002944957.2
CDS	173..2299
Translation	MDSKKTPNRYHLACKFCLKVTGTLPNHLRRACMKHADNQEIEFVMQEARGKMRAILEGLSIVNYEDLDYEDSDPKQFFVQFLEKNGCYIRGKLESASCAMKESILPSNMDRNKVNVNWPADEVGETQAPCKDLDLSHKMISPSPLNAENGQKQQPTLQSLQESIDFLMSQLAKKHEKVNSRPLNAEAVQKKRPVVQQGLPIESDEEDYVSLVNEQEVEMQGPELDLPIESDEENFISPVNEQEVEMQGPELDLPVESEENNADPLLDDNDDDDYDDDDKENEEMETHNLVEGLEQTETNSNYILLRRRMMAAGMYRKHSLECGVLARFRKYLQQELQVTRWSQEVANVARFLYFVDPSKASLSFLTDTQKTLDFFTALEKLGNTHATMFSYLKHIKRFVRSQTDTLSSGSHQSEDLAGKCKRYLDMTATLQRRLSKGITARVVGKKFDYLTSAKKDPSECQRILVVARPIFDQIIKKAKMGVCLLEKEKSYVIYYLEALIVLKYLQRPGVVQNMTVEEWCKRMPSDCKTRMVIGVKRHKTSTQQVASILLEEEEEEWFDVYYKKIRPSFIRSGKCPQTFFISSTGEPIHSVTNDIARLHHKFKLRPVTSQEARRTMETYMVSHFQTDAQRNMFARLLGHSNVTAGRIYGEKTVDNMVEAAEMMKRAMHESQPSTSRCQEVPQKPLQEETPEVSRGLREEALKNFKPAS

Target ORF information:

RefSeq Version	XM_002944911.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mesendoderm nuclear factor, gene 2 (menf.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002944911.4

ORF Insert Sequence:

ATGGATTCTA AGAAGACACC AAACCGCTAT CATCTTGCCT GCAAGTTTTG CCTGAAGGTT 
ACAGGCACCT TGCCTAATCA TCTGAGGAGA GCCTGCATGA AACATGCAGA TAACCAAGAA 
ATTGAATTTG TGATGCAAGA AGCAAGAGGA AAGATGAGAG CAATCCTGGA AGGGCTAAGT 
ATTGTTAATT ATGAAGACCT GGACTATGAG GATTCTGATC CTAAACAATT TTTTGTCCAG 
TTCCTGGAGA AAAATGGCTG CTATATCAGA GGGAAGCTAG AGTCAGCAAG CTGTGCCATG 
AAAGAGAGCA TCCTTCCATC AAATATGGAC AGAAACAAGG TGAACGTCAA TTGGCCAGCA 
GATGAAGTAG GGGAGACACA GGCCCCTTGT AAAGATCTGG ACCTGTCACA TAAGATGATA 
AGCCCCAGCC CCTTGAATGC TGAAAATGGG CAGAAACAGC AACCTACACT TCAGTCACTT 
CAGGAGAGCA TTGACTTCCT AATGTCACAA TTGGCCAAAA AGCATGAGAA GGTAAACTCC 
AGACCCTTGA ATGCTGAAGC AGTGCAGAAA AAGCGACCTG TTGTACAACA GGGCCTACCC 
ATCGAGTCAG ATGAGGAGGA CTATGTAAGC CTTGTGAATG AACAAGAGGT GGAGATGCAG 
GGACCTGAGC TTGACCTCCC CATCGAGTCA GATGAGGAGA ACTTCATAAG CCCTGTGAAT 
GAACAAGAGG TGGAGATGCA GGGACCTGAG CTTGACCTCC CTGTTGAGTC AGAGGAGAAC 
AATGCAGACC CCTTGCTTGA TGACAACGAT GATGATGATT ATGATGATGA CGACAAGGAA 
AATGAAGAGA TGGAGACACA CAATCTTGTT GAAGGGCTGG AACAGACCGA GACCAATTCA 
AACTACATTC TGCTTAGAAG AAGAATGATG GCTGCAGGCA TGTATAGAAA ACACTCTCTT 
GAATGTGGTG TTCTTGCTCG CTTCCGAAAA TATCTGCAGC AAGAATTACA AGTAACAAGA 
TGGTCACAAG AGGTGGCAAA TGTGGCCAGG TTTTTGTATT TTGTGGACCC CTCCAAGGCG 
TCCTTAAGCT TCCTGACCGA CACACAGAAG ACGTTGGATT TTTTTACGGC ACTTGAGAAA 
TTGGGAAACA CTCACGCCAC AATGTTTTCG TACCTCAAAC ATATCAAGAG GTTTGTGAGA 
TCCCAAACAG ACACTTTGTC CAGCGGATCC CATCAAAGTG AAGACCTTGC CGGAAAATGC 
AAACGCTATC TCGACATGAC CGCAACTCTA CAGCGAAGGC TGAGCAAGGG AATTACGGCC 
AGAGTTGTGG GGAAAAAGTT TGACTATCTG ACGTCTGCAA AAAAGGACCC ATCAGAGTGT 
CAACGGATCC TCGTAGTGGC CAGACCAATA TTTGACCAGA TAATAAAGAA GGCCAAGATG 
GGTGTATGTC TTTTGGAGAA GGAAAAGTCT TATGTCATCT ATTACTTGGA GGCACTCATT 
GTTTTAAAAT ACCTCCAGCG ACCAGGGGTT GTTCAAAATA TGACTGTAGA AGAATGGTGC 
AAGAGGATGC CATCTGATTG CAAAACCCGT ATGGTCATTG GTGTAAAAAG GCATAAGACA 
TCCACTCAGC AAGTCGCCTC TATACTGCTA GAAGAAGAAG AAGAAGAGTG GTTTGATGTT 
TATTATAAAA AAATACGGCC ATCATTTATC AGGAGTGGGA AATGCCCACA GACATTTTTC 
ATATCGTCAA CAGGGGAACC AATTCACAGT GTGACAAATG ACATCGCCCG GCTTCATCAT 
AAGTTTAAAT TGCGCCCTGT CACAAGTCAA GAGGCTAGAA GGACAATGGA GACATACATG 
GTCTCCCACT TTCAAACTGA TGCTCAGAGG AACATGTTTG CCAGGTTGCT TGGCCATTCA 
AATGTGACTG CCGGAAGGAT ATATGGCGAG AAGACCGTTG ACAATATGGT AGAAGCGGCA 
GAAATGATGA AGAGGGCGAT GCATGAATCC CAGCCATCAA CATCAAGGTG TCAAGAGGTG 
CCACAGAAGC CCCTGCAAGA AGAAACACCA GAAGTCTCCC GTGGGTTGAG GGAGGAAGCC 
TTGAAAAATT TCAAGCCTGC GAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

menf.2 ( XM_031906504.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031762364.1 Xenopus tropicalis mesendoderm nuclear factor, gene 2 (menf.2), mRNA.

Price & Availability↑

CloneID	OXa63013
Clone ID Related Accession (Same CDS sequence)	XM_031906504.1
Accession Version	XM_031906504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LOW QUALITY PROTEIN: uncharacterized protein menf.2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 17% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGATTCTA AGAAGACACC AAACCGCTAT CATCTTGCCT GCAAGTTTTG CCTGAAGGTT 
ACAGGCACCT TGCCTAATCA TCTGAGGAGA GCCTGCATGA AACATGCAGA TAACCAAGAA 
ATTGAATTTG TGATGCAAGA AGCAAGAGGA AAGATGAGAG CAATCCTGGA AGGGCTAAGT 
ATTGTTAATT ATGAAGACCT GGACTATGAG GATTCTGATC CTAAACAATT TTTTGTCCAG 
TTCCTGGAGA AAAATGGCTG CTATATCAGA GGGAAGCTAG AGTCAGCAAG CTGTGCCATG 
AAAGAGAGCA TCCTTCCATC AAATATGGAC AGAAACAAGG TGAACGTCAA TTGGCCAGCA 
GATGAAGTAG GGGAGACACA GGCCCCTTGT AAAGATCTGG ACCTGTCACA TAAGATGATA 
AGCCCCAGCC CCTTGAATGC TGAAAATGGG CAGAAACAGC AACCTACACT TCAGTCACTT 
CAGGAGAGCA TTGACTTCCT AATGTCACAA TTGGCCAAAA AGCATGAGAA GGTAAACTCC 
AGACCCTTGA ATGCTGAAGC AGTGCAGAAA AAGCGACCTG TTGTACAACA GGGCCTACCC 
ATCGAGTCAG ATGAGGAGGA CTATGTAAGC CTTGTGAATG AACAAGAGGT GGAGATGCAG 
GGACCTGAGC TTGACCTCCC CATCGAGTCA GATGAGGAGA ACTTCATAAG CCCTGTGAAT 
GAACAAGAGG TGGAGATGCA GGGACCTGAG CTTGACCTCC CTGTTGAGTC AGAGGAGAAC 
AATGCAGACC CCTTGCTTGA TGACAACGAT GATGATGATT ATGATGATGA CGACAAGGAA 
AATGAAGAGA TGGAGACACA CAATCTTGTT GAAGGGCTGG AACAGACCGA GACCAATTCA 
AACTACATTC TGCTTAGAAG AAGAATGATG GCTGCAGGCA TGTATAGAAA ACACTCTCTT 
GAATGTGGTG TTCTTGCTCG CTTCCGAAAA TATCTGCAGC AAGAATTACA AGTAACAAGA 
TGGTCACAAG AGGTGGCAAA TGTGGCCAGG TTTTTGTATT TTGTGGACCC CTCCAAGGCG 
TCCTTAAGCT TCCTGACCGA CACACAGAAG ACGTTGGATT TTTTTACGGC ACTTGAGAAA 
TTGGGAAACA CTCACGCCAC AATGTTTTCG TACCTCAAAC ATATCAAGAG GTTTGTGAGA 
TCCCAAACAG ACACTTTGTC CAGCGGATCC CATCAAAGTG AAGACCTTGC CGGAAAATGC 
AAACGCTATC TCGACATGAC CGCAACTCTA CAGCGAAGGC TGAGCAAGGG AATTACGGCC 
AGAGTTGTGG GGAAAAAGTT TGACTATCTG ACGTCTGCAA AAAAGGACCC ATCAGAGTGT 
CAACGGATCC TCGTAGTGGC CAGACCAATA TTTGACCAGA TAATAAAGAA GGCCAAGATG 
GGTGTATGTC TTTTGGAGAA GGAAAAGTCT TATGTCATCT ATTACTTGGA GGCACTCATT 
GTTTTAAAAT ACCTCCAGCG ACCAGGGGTT GTTCAAAATA TGACTGTAGA AGAATGGTGC 
AAGAGGATGC CATCTGATTG CAAAACCCGT ATGGTCATTG GTGTAAAAAG GCATAAGACA 
TCCACTCAGC AAGTCGCCTC TATACTGCTA GAAGAAGAAG AAGAAGAGTG GTTTGATGTT 
TATTATAAAA AAATACGGCC ATCATTTATC AGGAGTGGGA AATGCCCACA GACATTTTTC 
ATATCGTCAA CAGGGGAACC AATTCACAGT GTGACAAATG ACATCGCCCG GCTTCATCAT 
AAGTTTAAAT TGCGCCCTGT CACAAGTCAA GAGGCTAGAA GGACAATGGA GACATACATG 
GTCTCCCACT TTCAAACTGA TGCTCAGAGG AACATGTTTG CCAGGTTGCT TGGCCATTCA 
AATGTGACTG CCGGAAGGAT ATATGGCGAG AAGACCGTTG ACAATATGGT AGAAGCGGCA 
GAAATGATGA AGAGGGCGAT GCATGAATCC CAGCCATCAA CATCAAGGTG TCAAGAGGTG 
CCACAGAAGC CCCTGCAAGA AGAAACACCA GAAGTCTCCC GTGGGTTGAG GGAGGAAGCC 
TTTGAAAAAT TTAAAAAGTG CCACCCACTT ACTATTGATG CCACGCCTCC TTGCTTAAAG 
GCAGCACTAG GCTTTTCCCG TGAGTACGGC CAGTACGTTT ATGACCGTTG GAGAAAACAG 
CAAAATAAGA TGAGAGTGGA TTATGTTGCT GGACTTCTCA AATATGAGAA TCCACATGAA 
GAAAGANGAT GTATCCAAGG CACTGGATGG AAGACCAACT TGCCACTGAT AAGGGATATT 
TTGGAAACCT GTAACCCTCG GTCTAAAAAG AAGCACAACT TGGATATCAG AATGGCCATA 
AATTCATCCA GAATAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031762364.1
CDS	1..2421
Translation	MDSKKTPNRYHLACKFCLKVTGTLPNHLRRACMKHADNQEIEFVMQEARGKMRAILEGLSIVNYEDLDYEDSDPKQFFVQFLEKNGCYIRGKLESASCAMKESILPSNMDRNKVNVNWPADEVGETQAPCKDLDLSHKMISPSPLNAENGQKQQPTLQSLQESIDFLMSQLAKKHEKVNSRPLNAEAVQKKRPVVQQGLPIESDEEDYVSLVNEQEVEMQGPELDLPIESDEENFISPVNEQEVEMQGPELDLPVESEENNADPLLDDNDDDDYDDDDKENEEMETHNLVEGLEQTETNSNYILLRRRMMAAGMYRKHSLECGVLARFRKYLQQELQVTRWSQEVANVARFLYFVDPSKASLSFLTDTQKTLDFFTALEKLGNTHATMFSYLKHIKRFVRSQTDTLSSGSHQSEDLAGKCKRYLDMTATLQRRLSKGITARVVGKKFDYLTSAKKDPSECQRILVVARPIFDQIIKKAKMGVCLLEKEKSYVIYYLEALIVLKYLQRPGVVQNMTVEEWCKRMPSDCKTRMVIGVKRHKTSTQQVASILLEEEEEEWFDVYYKKIRPSFIRSGKCPQTFFISSTGEPIHSVTNDIARLHHKFKLRPVTSQEARRTMETYMVSHFQTDAQRNMFARLLGHSNVTAGRIYGEKTVDNMVEAAEMMKRAMHESQPSTSRCQEVPQKPLQEETPEVSRGLREEAFEKFKKCHPLTIDATPPCLKAALGFSREYGQYVYDRWRKQQNKMRVDYVAGLLKYENPHEERnullCIQGTGWKTNLPLIRDILETCNPRSKKKHNLDIRMAINSSRIR

Target ORF information:

RefSeq Version	XM_031906504.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mesendoderm nuclear factor, gene 2 (menf.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906504.1

ORF Insert Sequence:

ATGGATTCTA AGAAGACACC AAACCGCTAT CATCTTGCCT GCAAGTTTTG CCTGAAGGTT 
ACAGGCACCT TGCCTAATCA TCTGAGGAGA GCCTGCATGA AACATGCAGA TAACCAAGAA 
ATTGAATTTG TGATGCAAGA AGCAAGAGGA AAGATGAGAG CAATCCTGGA AGGGCTAAGT 
ATTGTTAATT ATGAAGACCT GGACTATGAG GATTCTGATC CTAAACAATT TTTTGTCCAG 
TTCCTGGAGA AAAATGGCTG CTATATCAGA GGGAAGCTAG AGTCAGCAAG CTGTGCCATG 
AAAGAGAGCA TCCTTCCATC AAATATGGAC AGAAACAAGG TGAACGTCAA TTGGCCAGCA 
GATGAAGTAG GGGAGACACA GGCCCCTTGT AAAGATCTGG ACCTGTCACA TAAGATGATA 
AGCCCCAGCC CCTTGAATGC TGAAAATGGG CAGAAACAGC AACCTACACT TCAGTCACTT 
CAGGAGAGCA TTGACTTCCT AATGTCACAA TTGGCCAAAA AGCATGAGAA GGTAAACTCC 
AGACCCTTGA ATGCTGAAGC AGTGCAGAAA AAGCGACCTG TTGTACAACA GGGCCTACCC 
ATCGAGTCAG ATGAGGAGGA CTATGTAAGC CTTGTGAATG AACAAGAGGT GGAGATGCAG 
GGACCTGAGC TTGACCTCCC CATCGAGTCA GATGAGGAGA ACTTCATAAG CCCTGTGAAT 
GAACAAGAGG TGGAGATGCA GGGACCTGAG CTTGACCTCC CTGTTGAGTC AGAGGAGAAC 
AATGCAGACC CCTTGCTTGA TGACAACGAT GATGATGATT ATGATGATGA CGACAAGGAA 
AATGAAGAGA TGGAGACACA CAATCTTGTT GAAGGGCTGG AACAGACCGA GACCAATTCA 
AACTACATTC TGCTTAGAAG AAGAATGATG GCTGCAGGCA TGTATAGAAA ACACTCTCTT 
GAATGTGGTG TTCTTGCTCG CTTCCGAAAA TATCTGCAGC AAGAATTACA AGTAACAAGA 
TGGTCACAAG AGGTGGCAAA TGTGGCCAGG TTTTTGTATT TTGTGGACCC CTCCAAGGCG 
TCCTTAAGCT TCCTGACCGA CACACAGAAG ACGTTGGATT TTTTTACGGC ACTTGAGAAA 
TTGGGAAACA CTCACGCCAC AATGTTTTCG TACCTCAAAC ATATCAAGAG GTTTGTGAGA 
TCCCAAACAG ACACTTTGTC CAGCGGATCC CATCAAAGTG AAGACCTTGC CGGAAAATGC 
AAACGCTATC TCGACATGAC CGCAACTCTA CAGCGAAGGC TGAGCAAGGG AATTACGGCC 
AGAGTTGTGG GGAAAAAGTT TGACTATCTG ACGTCTGCAA AAAAGGACCC ATCAGAGTGT 
CAACGGATCC TCGTAGTGGC CAGACCAATA TTTGACCAGA TAATAAAGAA GGCCAAGATG 
GGTGTATGTC TTTTGGAGAA GGAAAAGTCT TATGTCATCT ATTACTTGGA GGCACTCATT 
GTTTTAAAAT ACCTCCAGCG ACCAGGGGTT GTTCAAAATA TGACTGTAGA AGAATGGTGC 
AAGAGGATGC CATCTGATTG CAAAACCCGT ATGGTCATTG GTGTAAAAAG GCATAAGACA 
TCCACTCAGC AAGTCGCCTC TATACTGCTA GAAGAAGAAG AAGAAGAGTG GTTTGATGTT 
TATTATAAAA AAATACGGCC ATCATTTATC AGGAGTGGGA AATGCCCACA GACATTTTTC 
ATATCGTCAA CAGGGGAACC AATTCACAGT GTGACAAATG ACATCGCCCG GCTTCATCAT 
AAGTTTAAAT TGCGCCCTGT CACAAGTCAA GAGGCTAGAA GGACAATGGA GACATACATG 
GTCTCCCACT TTCAAACTGA TGCTCAGAGG AACATGTTTG CCAGGTTGCT TGGCCATTCA 
AATGTGACTG CCGGAAGGAT ATATGGCGAG AAGACCGTTG ACAATATGGT AGAAGCGGCA 
GAAATGATGA AGAGGGCGAT GCATGAATCC CAGCCATCAA CATCAAGGTG TCAAGAGGTG 
CCACAGAAGC CCCTGCAAGA AGAAACACCA GAAGTCTCCC GTGGGTTGAG GGAGGAAGCC 
TTTGAAAAAT TTAAAAAGTG CCACCCACTT ACTATTGATG CCACGCCTCC TTGCTTAAAG 
GCAGCACTAG GCTTTTCCCG TGAGTACGGC CAGTACGTTT ATGACCGTTG GAGAAAACAG 
CAAAATAAGA TGAGAGTGGA TTATGTTGCT GGACTTCTCA AATATGAGAA TCCACATGAA 
GAAAGANGAT GTATCCAAGG CACTGGATGG AAGACCAACT TGCCACTGAT AAGGGATATT 
TTGGAAACCT GTAACCCTCG GTCTAAAAAG AAGCACAACT TGGATATCAG AATGGCCATA 
AATTCATCCA GAATAAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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menf.2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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