top1.2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	top1.2
Entrez Gene ID	100380116
Full Name	DNA topoisomerase I, gene 2
Synonyms	topi
General protein information	Preferred Names DNA topoisomerase I, gene 2 Names topoisomerase (DNA) I, gene 2 DNA topoisomerase 1 topoisomerase DNA 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018092953.2	XP_017948442.2	topoisomerase (DNA) I, gene 2 isoform X2
XM_018092952.1	XP_017948441.1	topoisomerase (DNA) I, gene 2 isoform X1
XM_018092953.1	XP_017948442.1	topoisomerase (DNA) I, gene 2 isoform X2
XM_018092954.1	XP_017948443.1	topoisomerase (DNA) I, gene 2 isoform X3
XM_018092952.2	XP_017948441.2	topoisomerase (DNA) I, gene 2 isoform X1
XM_031899929.1	XP_031755789.1	topoisomerase (DNA) I, gene 2 isoform X3
NM_001195692.2	NP_001182621.1	topoisomerase (DNA) I, gene 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	zgc:173742	XP_002665372.2
Xenopus tropicalis (tropical clawed frog)	top1.2	NP_001182621.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following top1.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top1.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa20424	NM_001195692.2 Latest version!	Xenopus tropicalis DNA topoisomerase I, gene 2 (top1.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa20424	XM_018092952.1 Latest version!	Xenopus tropicalis topoisomerase (DNA) I, gene 2 (top1.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa43745	XM_018092953.1 Latest version!	Xenopus tropicalis topoisomerase (DNA) I, gene 2 (top1.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa43746	XM_018092954.1 Latest version!	Xenopus tropicalis topoisomerase (DNA) I, gene 2 (top1.2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa55868	XM_018092952.2 Latest version!	Xenopus tropicalis DNA topoisomerase I, gene 2 (top1.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa55869	XM_018092953.2 Latest version!	Xenopus tropicalis DNA topoisomerase I, gene 2 (top1.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa55870	XM_031899929.1 Latest version!	Xenopus tropicalis DNA topoisomerase I, gene 2 (top1.2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

top1.2 ( NM_001195692.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001182621.1 Xenopus tropicalis DNA topoisomerase I, gene 2 (top1.2), mRNA.

Price & Availability↑

CloneID	OXa20424
Clone ID Related Accession (Same CDS sequence)	XM_018092952.1 , NM_001195692.2
Accession Version	NM_001195692.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	topoisomerase (DNA) I, gene 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL846716.2, BC121625.1, DR848249.1 and AL871340.2. On Aug 10, 2013 this sequence version replaced NM_001195692.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGACACAGC TGTCGCTGCC AGTTGAAGAT CTTGTTTCTT TTCATAATCT ACCTGAGAAA 
GCAAGAGACA AAAAAAAGAA ACTAAAAAGT GAGCCAAAAC CTGAAGGGAA GGCCAAAAAG 
AAGGACAAAG GAAAAGCAGC AGTGCAGGAT GGCGAAACTC AAAGACAAAG GGGGAAAGAG 
AATAACATGC CAGTAGCTGA AGAGAGAGAA ATAAAATCGG ATAAAAGAAA AGAAAAAAAG 
AAAAAAGATA GCAAGAAAAA AAAGGAAAAG AATTCATCTG AGCACTGTGA AATTCAGCAA 
CAAGACCCTC AGACAAGTTC TGTGATTGTC AAATTAGAAC CTGATGAAGA TGGAGACTTT 
GCACCTGCTA AAAAACAAAG TAAAGGGAAA AAACGAGCAA GACAAGGGGA AGCTGGAGAA 
TTGTCAAAGT CTAAGAAAGT GAAACTAGAG GAAGATGCAG ATGGAGTTGA AACAGAAACT 
AAACCACAAA AAAAGAAAAA AATTAAAAAG GAAGAAGGTA ATAAAAAACC CAAGAAGGAA 
GAAGGTGCAA TCAAGGCAAA AAAGAAAACA CAAGAGGAGA AGGAAGCAGC AGCTGCTTTG 
AAATTAAAGA AAAAAGAAGA AGAAGAGCAG ATGCGTTGGA GATGGTGGGA GGAAGAAAAG 
TACGAAGATG GCATAAAGTG GAAGTTTCTG GAACATGAAG GTCCTGTTTT TGCTCCACCC 
TATGAGCCAT TGCCGGATAG TGTGAAATTT TACTATGATG GTAAACCCCT TCCATTAAGT 
CTACAAGCAG AAGAAGTGGC TAGTTTCTTT GCAAAAATGT TAGACCACGA GTATACAACC 
AAGGAAATCT TTCGCAATAA CTACTTTATG GATTGGAGAA AGGAGATGAC ATTGGAAGAA 
AGGATGGTGA TTACTGACCT GGCCAAATGT GATTTTACTG AAATGTTTAC TTACTACAAA 
GGAAAAATGG AGGAAAAGAA AAATAAATCA AAAGAAGATA AACTAAAAAT CAAAGAGGAA 
AATGAAAAAC TGTTGCAAGA GTATGGTTAC TGTAGCATGG ATCACCATCG AGAGAGAATT 
GGAAACTTTA AGATTGAGCC TCCAGCACTA TTCCGTGGAC GAGGAGACCA CCCCAAACAG 
GGGATGTTGA AGAAGCGGAT ACTCCCTGAA GATATTATAA TCAATTGTAG CAAAGACTCA 
AAAATCCCTG AGCCACCTCC TGGGCACAAA TGGAAAGAGG TGCGCCATGA CAACACTGTT 
ACCTGGTTGG CATCATGGAC AGAAAATGTA CAAGGATCAG TGAAGTACAT CATGCTCAAT 
GCAAACTCCA AGTTGAAGGG AGAAAAAGAC TGGGAGAAGT ATGAAGTTGC TAGGAAGCTG 
AAACATCATG TTGTAACAAT TAGGAAGACA TACCGGGAAA ATTTTAAATC CAAGGAAATG 
AAAGTGCGTC AGCGGGCCGT GGCTCTGTAC TTCATTGATA AGCTGGCTTT GAGAGCAGGT 
AATGAAAAGG AAGAAGGAGA GACTGCTGAT ACAGTTGGTT GTTGCTCCCT AAGAGTGGAG 
CACATAAAGC TTTACCCAGA ACTTGATGGA GACAAGTATG TAGTTGAGTT TGACTTCCTG 
GGTAAAGACT CTATTCGATA CTATAACAAA GTTCCTGTGG AAAAGCAGGT CTTTAAAAAC 
CTCCAGCTGT TTATGGAGAA CAAGCAGGAA GGAGATGATC TATTTGACAG GCTGTCTACA 
CCTTTGCTTA ACAAGCACCT GCAGTCACTG ATGGAAGGAC TGACTGCAAA GGTTTTCAGG 
ACATACAACG CATCGATTAC ACTGCAGCAG CAGCTCCAGG AGCTCACAGA CCCTGAAGAA 
AGCATGCCTT CAAAGATCTT ATCATATAAC AGAGCTAACA GAGCGGTTGC AATTCTGTGC 
AACCATCAGA GGGCACCACC AAAAACCTTT GATCAATCTA TGGCAAATCT TAAATCTAAG 
ATTGATGCGA AGAAAGAACA GATTTTACTG GCAAAGAAAG AGCTGAAAGA AGCCAAAAAA 
GAGGCCAAAG AAAAAGAGAG TGAGAAGCTT AATAAAGTTG TAGAGTCTAA GAAAAGAGGA 
GTGCAGAGAG CAGAGGAGCA ACTCATGAGA ATGGAGGTGC AGGCAACTGA CCGAGAAGAG 
AATAAACAGA TTGCCTTGAG CACCTCCAAA CTAAACTATC TGGATCCCAG GATCTCTGTG 
GCCTGGTGCA AGAAATGGAC TGTTGGACTG GAAAAAATCT ACAACAAGAC GTACCGGGAC 
AAGTTTGCTT GGGCCGTTGA CATGACCGAA ACCGATTTTA TATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182621.1
CDS	23..2350
Translation	MTQLSLPVEDLVSFHNLPEKARDKKKKLKSEPKPEGKAKKKDKGKAAVQDGETQRQRGKENNMPVAEEREIKSDKRKEKKKKDSKKKKEKNSSEHCEIQQQDPQTSSVIVKLEPDEDGDFAPAKKQSKGKKRARQGEAGELSKSKKVKLEEDADGVETETKPQKKKKIKKEEGNKKPKKEEGAIKAKKKTQEEKEAAAALKLKKKEEEEQMRWRWWEEEKYEDGIKWKFLEHEGPVFAPPYEPLPDSVKFYYDGKPLPLSLQAEEVASFFAKMLDHEYTTKEIFRNNYFMDWRKEMTLEERMVITDLAKCDFTEMFTYYKGKMEEKKNKSKEDKLKIKEENEKLLQEYGYCSMDHHRERIGNFKIEPPALFRGRGDHPKQGMLKKRILPEDIIINCSKDSKIPEPPPGHKWKEVRHDNTVTWLASWTENVQGSVKYIMLNANSKLKGEKDWEKYEVARKLKHHVVTIRKTYRENFKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHIKLYPELDGDKYVVEFDFLGKDSIRYYNKVPVEKQVFKNLQLFMENKQEGDDLFDRLSTPLLNKHLQSLMEGLTAKVFRTYNASITLQQQLQELTDPEESMPSKILSYNRANRAVAILCNHQRAPPKTFDQSMANLKSKIDAKKEQILLAKKELKEAKKEAKEKESEKLNKVVESKKRGVQRAEEQLMRMEVQATDREENKQIALSTSKLNYLDPRISVAWCKKWTVGLEKIYNKTYRDKFAWAVDMTETDFIF

Target ORF information:

RefSeq Version	NM_001195692.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DNA topoisomerase I, gene 2 (top1.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195692.2

ORF Insert Sequence:

ATGACACAGC TGTCGCTGCC AGTTGAAGAT CTTGTTTCTT TTCATAATCT ACCTGAGAAA 
GCAAGAGACA AAAAAAAGAA ACTAAAAAGT GAGCCAAAAC CTGAAGGGAA GGCCAAAAAG 
AAGGACAAAG GAAAAGCAGC AGTGCAGGAT GGCGAAACTC AAAGACAAAG GGGGAAAGAG 
AATAACATGC CAGTAGCTGA AGAGAGAGAA ATAAAATCGG ATAAAAGAAA AGAAAAAAAG 
AAAAAAGATA GCAAGAAAAA AAAGGAAAAG AATTCATCTG AGCACTGTGA AATTCAGCAA 
CAAGACCCTC AGACAAGTTC TGTGATTGTC AAATTAGAAC CTGATGAAGA TGGAGACTTT 
GCACCTGCTA AAAAACAAAG TAAAGGGAAA AAACGAGCAA GACAAGGGGA AGCTGGAGAA 
TTGTCAAAGT CTAAGAAAGT GAAACTAGAG GAAGATGCAG ATGGAGTTGA AACAGAAACT 
AAACCACAAA AAAAGAAAAA AATTAAAAAG GAAGAAGGTA ATAAAAAACC CAAGAAGGAA 
GAAGGTGCAA TCAAGGCAAA AAAGAAAACA CAAGAGGAGA AGGAAGCAGC AGCTGCTTTG 
AAATTAAAGA AAAAAGAAGA AGAAGAGCAG ATGCGTTGGA GATGGTGGGA GGAAGAAAAG 
TACGAAGATG GCATAAAGTG GAAGTTTCTG GAACATGAAG GTCCTGTTTT TGCTCCACCC 
TATGAGCCAT TGCCGGATAG TGTGAAATTT TACTATGATG GTAAACCCCT TCCATTAAGT 
CTACAAGCAG AAGAAGTGGC TAGTTTCTTT GCAAAAATGT TAGACCACGA GTATACAACC 
AAGGAAATCT TTCGCAATAA CTACTTTATG GATTGGAGAA AGGAGATGAC ATTGGAAGAA 
AGGATGGTGA TTACTGACCT GGCCAAATGT GATTTTACTG AAATGTTTAC TTACTACAAA 
GGAAAAATGG AGGAAAAGAA AAATAAATCA AAAGAAGATA AACTAAAAAT CAAAGAGGAA 
AATGAAAAAC TGTTGCAAGA GTATGGTTAC TGTAGCATGG ATCACCATCG AGAGAGAATT 
GGAAACTTTA AGATTGAGCC TCCAGCACTA TTCCGTGGAC GAGGAGACCA CCCCAAACAG 
GGGATGTTGA AGAAGCGGAT ACTCCCTGAA GATATTATAA TCAATTGTAG CAAAGACTCA 
AAAATCCCTG AGCCACCTCC TGGGCACAAA TGGAAAGAGG TGCGCCATGA CAACACTGTT 
ACCTGGTTGG CATCATGGAC AGAAAATGTA CAAGGATCAG TGAAGTACAT CATGCTCAAT 
GCAAACTCCA AGTTGAAGGG AGAAAAAGAC TGGGAGAAGT ATGAAGTTGC TAGGAAGCTG 
AAACATCATG TTGTAACAAT TAGGAAGACA TACCGGGAAA ATTTTAAATC CAAGGAAATG 
AAAGTGCGTC AGCGGGCCGT GGCTCTGTAC TTCATTGATA AGCTGGCTTT GAGAGCAGGT 
AATGAAAAGG AAGAAGGAGA GACTGCTGAT ACAGTTGGTT GTTGCTCCCT AAGAGTGGAG 
CACATAAAGC TTTACCCAGA ACTTGATGGA GACAAGTATG TAGTTGAGTT TGACTTCCTG 
GGTAAAGACT CTATTCGATA CTATAACAAA GTTCCTGTGG AAAAGCAGGT CTTTAAAAAC 
CTCCAGCTGT TTATGGAGAA CAAGCAGGAA GGAGATGATC TATTTGACAG GCTGTCTACA 
CCTTTGCTTA ACAAGCACCT GCAGTCACTG ATGGAAGGAC TGACTGCAAA GGTTTTCAGG 
ACATACAACG CATCGATTAC ACTGCAGCAG CAGCTCCAGG AGCTCACAGA CCCTGAAGAA 
AGCATGCCTT CAAAGATCTT ATCATATAAC AGAGCTAACA GAGCGGTTGC AATTCTGTGC 
AACCATCAGA GGGCACCACC AAAAACCTTT GATCAATCTA TGGCAAATCT TAAATCTAAG 
ATTGATGCGA AGAAAGAACA GATTTTACTG GCAAAGAAAG AGCTGAAAGA AGCCAAAAAA 
GAGGCCAAAG AAAAAGAGAG TGAGAAGCTT AATAAAGTTG TAGAGTCTAA GAAAAGAGGA 
GTGCAGAGAG CAGAGGAGCA ACTCATGAGA ATGGAGGTGC AGGCAACTGA CCGAGAAGAG 
AATAAACAGA TTGCCTTGAG CACCTCCAAA CTAAACTATC TGGATCCCAG GATCTCTGTG 
GCCTGGTGCA AGAAATGGAC TGTTGGACTG GAAAAAATCT ACAACAAGAC GTACCGGGAC 
AAGTTTGCTT GGGCCGTTGA CATGACCGAA ACCGATTTTA TATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

top1.2 ( XM_018092952.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948441.1 Xenopus tropicalis topoisomerase (DNA) I, gene 2 (top1.2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa20424
Clone ID Related Accession (Same CDS sequence)	XM_018092952.1 , NM_001195692.2
Accession Version	XM_018092952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	topoisomerase (DNA) I, gene 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACAGC TGTCGCTGCC AGTTGAAGAT CTTGTTTCTT TTCATAATCT ACCTGAGAAA 
GCAAGAGACA AAAAAAAGAA ACTAAAAAGT GAGCCAAAAC CTGAAGGGAA GGCCAAAAAG 
AAGGACAAAG GAAAAGCAGC AGTGCAGGAT GGCGAAACTC AAAGACAAAG GGGGAAAGAG 
AATAACATGC CAGTAGCTGA AGAGAGAGAA ATAAAATCGG ATAAAAGAAA AGAAAAAAAG 
AAAAAAGATA GCAAGAAAAA AAAGGAAAAG AATTCATCTG AGCACTGTGA AATTCAGCAA 
CAAGACCCTC AGACAAGTTC TGTGATTGTC AAATTAGAAC CTGATGAAGA TGGAGACTTT 
GCACCTGCTA AAAAACAAAG TAAAGGGAAA AAACGAGCAA GACAAGGGGA AGCTGGAGAA 
TTGTCAAAGT CTAAGAAAGT GAAACTAGAG GAAGATGCAG ATGGAGTTGA AACAGAAACT 
AAACCACAAA AAAAGAAAAA AATTAAAAAG GAAGAAGGTA ATAAAAAACC CAAGAAGGAA 
GAAGGTGCAA TCAAGGCAAA AAAGAAAACA CAAGAGGAGA AGGAAGCAGC AGCTGCTTTG 
AAATTAAAGA AAAAAGAAGA AGAAGAGCAG ATGCGTTGGA GATGGTGGGA GGAAGAAAAG 
TACGAAGATG GCATAAAGTG GAAGTTTCTG GAACATGAAG GTCCTGTTTT TGCTCCACCC 
TATGAGCCAT TGCCGGATAG TGTGAAATTT TACTATGATG GTAAACCCCT TCCATTAAGT 
CTACAAGCAG AAGAAGTGGC TAGTTTCTTT GCAAAAATGT TAGACCACGA GTATACAACC 
AAGGAAATCT TTCGCAATAA CTACTTTATG GATTGGAGAA AGGAGATGAC ATTGGAAGAA 
AGGATGGTGA TTACTGACCT GGCCAAATGT GATTTTACTG AAATGTTTAC TTACTACAAA 
GGAAAAATGG AGGAAAAGAA AAATAAATCA AAAGAAGATA AACTAAAAAT CAAAGAGGAA 
AATGAAAAAC TGTTGCAAGA GTATGGTTAC TGTAGCATGG ATCACCATCG AGAGAGAATT 
GGAAACTTTA AGATTGAGCC TCCAGCACTA TTCCGTGGAC GAGGAGACCA CCCCAAACAG 
GGGATGTTGA AGAAGCGGAT ACTCCCTGAA GATATTATAA TCAATTGTAG CAAAGACTCA 
AAAATCCCTG AGCCACCTCC TGGGCACAAA TGGAAAGAGG TGCGCCATGA CAACACTGTT 
ACCTGGTTGG CATCATGGAC AGAAAATGTA CAAGGATCAG TGAAGTACAT CATGCTCAAT 
GCAAACTCCA AGTTGAAGGG AGAAAAAGAC TGGGAGAAGT ATGAAGTTGC TAGGAAGCTG 
AAACATCATG TTGTAACAAT TAGGAAGACA TACCGGGAAA ATTTTAAATC CAAGGAAATG 
AAAGTGCGTC AGCGGGCCGT GGCTCTGTAC TTCATTGATA AGCTGGCTTT GAGAGCAGGT 
AATGAAAAGG AAGAAGGAGA GACTGCTGAT ACAGTTGGTT GTTGCTCCCT AAGAGTGGAG 
CACATAAAGC TTTACCCAGA ACTTGATGGA GACAAGTATG TAGTTGAGTT TGACTTCCTG 
GGTAAAGACT CTATTCGATA CTATAACAAA GTTCCTGTGG AAAAGCAGGT CTTTAAAAAC 
CTCCAGCTGT TTATGGAGAA CAAGCAGGAA GGAGATGATC TATTTGACAG GCTGTCTACA 
CCTTTGCTTA ACAAGCACCT GCAGTCACTG ATGGAAGGAC TGACTGCAAA GGTTTTCAGG 
ACATACAACG CATCGATTAC ACTGCAGCAG CAGCTCCAGG AGCTCACAGA CCCTGAAGAA 
AGCATGCCTT CAAAGATCTT ATCATATAAC AGAGCTAACA GAGCGGTTGC AATTCTGTGC 
AACCATCAGA GGGCACCACC AAAAACCTTT GATCAATCTA TGGCAAATCT TAAATCTAAG 
ATTGATGCGA AGAAAGAACA GATTTTACTG GCAAAGAAAG AGCTGAAAGA AGCCAAAAAA 
GAGGCCAAAG AAAAAGAGAG TGAGAAGCTT AATAAAGTTG TAGAGTCTAA GAAAAGAGGA 
GTGCAGAGAG CAGAGGAGCA ACTCATGAGA ATGGAGGTGC AGGCAACTGA CCGAGAAGAG 
AATAAACAGA TTGCCTTGAG CACCTCCAAA CTAAACTATC TGGATCCCAG GATCTCTGTG 
GCCTGGTGCA AGAAATGGAC TGTTGGACTG GAAAAAATCT ACAACAAGAC GTACCGGGAC 
AAGTTTGCTT GGGCCGTTGA CATGACCGAA ACCGATTTTA TATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948441.1
CDS	100..2427
Translation	MTQLSLPVEDLVSFHNLPEKARDKKKKLKSEPKPEGKAKKKDKGKAAVQDGETQRQRGKENNMPVAEEREIKSDKRKEKKKKDSKKKKEKNSSEHCEIQQQDPQTSSVIVKLEPDEDGDFAPAKKQSKGKKRARQGEAGELSKSKKVKLEEDADGVETETKPQKKKKIKKEEGNKKPKKEEGAIKAKKKTQEEKEAAAALKLKKKEEEEQMRWRWWEEEKYEDGIKWKFLEHEGPVFAPPYEPLPDSVKFYYDGKPLPLSLQAEEVASFFAKMLDHEYTTKEIFRNNYFMDWRKEMTLEERMVITDLAKCDFTEMFTYYKGKMEEKKNKSKEDKLKIKEENEKLLQEYGYCSMDHHRERIGNFKIEPPALFRGRGDHPKQGMLKKRILPEDIIINCSKDSKIPEPPPGHKWKEVRHDNTVTWLASWTENVQGSVKYIMLNANSKLKGEKDWEKYEVARKLKHHVVTIRKTYRENFKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHIKLYPELDGDKYVVEFDFLGKDSIRYYNKVPVEKQVFKNLQLFMENKQEGDDLFDRLSTPLLNKHLQSLMEGLTAKVFRTYNASITLQQQLQELTDPEESMPSKILSYNRANRAVAILCNHQRAPPKTFDQSMANLKSKIDAKKEQILLAKKELKEAKKEAKEKESEKLNKVVESKKRGVQRAEEQLMRMEVQATDREENKQIALSTSKLNYLDPRISVAWCKKWTVGLEKIYNKTYRDKFAWAVDMTETDFIF

Target ORF information:

RefSeq Version	XM_018092952.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis topoisomerase (DNA) I, gene 2 (top1.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092952.1

ORF Insert Sequence:

ATGACACAGC TGTCGCTGCC AGTTGAAGAT CTTGTTTCTT TTCATAATCT ACCTGAGAAA 
GCAAGAGACA AAAAAAAGAA ACTAAAAAGT GAGCCAAAAC CTGAAGGGAA GGCCAAAAAG 
AAGGACAAAG GAAAAGCAGC AGTGCAGGAT GGCGAAACTC AAAGACAAAG GGGGAAAGAG 
AATAACATGC CAGTAGCTGA AGAGAGAGAA ATAAAATCGG ATAAAAGAAA AGAAAAAAAG 
AAAAAAGATA GCAAGAAAAA AAAGGAAAAG AATTCATCTG AGCACTGTGA AATTCAGCAA 
CAAGACCCTC AGACAAGTTC TGTGATTGTC AAATTAGAAC CTGATGAAGA TGGAGACTTT 
GCACCTGCTA AAAAACAAAG TAAAGGGAAA AAACGAGCAA GACAAGGGGA AGCTGGAGAA 
TTGTCAAAGT CTAAGAAAGT GAAACTAGAG GAAGATGCAG ATGGAGTTGA AACAGAAACT 
AAACCACAAA AAAAGAAAAA AATTAAAAAG GAAGAAGGTA ATAAAAAACC CAAGAAGGAA 
GAAGGTGCAA TCAAGGCAAA AAAGAAAACA CAAGAGGAGA AGGAAGCAGC AGCTGCTTTG 
AAATTAAAGA AAAAAGAAGA AGAAGAGCAG ATGCGTTGGA GATGGTGGGA GGAAGAAAAG 
TACGAAGATG GCATAAAGTG GAAGTTTCTG GAACATGAAG GTCCTGTTTT TGCTCCACCC 
TATGAGCCAT TGCCGGATAG TGTGAAATTT TACTATGATG GTAAACCCCT TCCATTAAGT 
CTACAAGCAG AAGAAGTGGC TAGTTTCTTT GCAAAAATGT TAGACCACGA GTATACAACC 
AAGGAAATCT TTCGCAATAA CTACTTTATG GATTGGAGAA AGGAGATGAC ATTGGAAGAA 
AGGATGGTGA TTACTGACCT GGCCAAATGT GATTTTACTG AAATGTTTAC TTACTACAAA 
GGAAAAATGG AGGAAAAGAA AAATAAATCA AAAGAAGATA AACTAAAAAT CAAAGAGGAA 
AATGAAAAAC TGTTGCAAGA GTATGGTTAC TGTAGCATGG ATCACCATCG AGAGAGAATT 
GGAAACTTTA AGATTGAGCC TCCAGCACTA TTCCGTGGAC GAGGAGACCA CCCCAAACAG 
GGGATGTTGA AGAAGCGGAT ACTCCCTGAA GATATTATAA TCAATTGTAG CAAAGACTCA 
AAAATCCCTG AGCCACCTCC TGGGCACAAA TGGAAAGAGG TGCGCCATGA CAACACTGTT 
ACCTGGTTGG CATCATGGAC AGAAAATGTA CAAGGATCAG TGAAGTACAT CATGCTCAAT 
GCAAACTCCA AGTTGAAGGG AGAAAAAGAC TGGGAGAAGT ATGAAGTTGC TAGGAAGCTG 
AAACATCATG TTGTAACAAT TAGGAAGACA TACCGGGAAA ATTTTAAATC CAAGGAAATG 
AAAGTGCGTC AGCGGGCCGT GGCTCTGTAC TTCATTGATA AGCTGGCTTT GAGAGCAGGT 
AATGAAAAGG AAGAAGGAGA GACTGCTGAT ACAGTTGGTT GTTGCTCCCT AAGAGTGGAG 
CACATAAAGC TTTACCCAGA ACTTGATGGA GACAAGTATG TAGTTGAGTT TGACTTCCTG 
GGTAAAGACT CTATTCGATA CTATAACAAA GTTCCTGTGG AAAAGCAGGT CTTTAAAAAC 
CTCCAGCTGT TTATGGAGAA CAAGCAGGAA GGAGATGATC TATTTGACAG GCTGTCTACA 
CCTTTGCTTA ACAAGCACCT GCAGTCACTG ATGGAAGGAC TGACTGCAAA GGTTTTCAGG 
ACATACAACG CATCGATTAC ACTGCAGCAG CAGCTCCAGG AGCTCACAGA CCCTGAAGAA 
AGCATGCCTT CAAAGATCTT ATCATATAAC AGAGCTAACA GAGCGGTTGC AATTCTGTGC 
AACCATCAGA GGGCACCACC AAAAACCTTT GATCAATCTA TGGCAAATCT TAAATCTAAG 
ATTGATGCGA AGAAAGAACA GATTTTACTG GCAAAGAAAG AGCTGAAAGA AGCCAAAAAA 
GAGGCCAAAG AAAAAGAGAG TGAGAAGCTT AATAAAGTTG TAGAGTCTAA GAAAAGAGGA 
GTGCAGAGAG CAGAGGAGCA ACTCATGAGA ATGGAGGTGC AGGCAACTGA CCGAGAAGAG 
AATAAACAGA TTGCCTTGAG CACCTCCAAA CTAAACTATC TGGATCCCAG GATCTCTGTG 
GCCTGGTGCA AGAAATGGAC TGTTGGACTG GAAAAAATCT ACAACAAGAC GTACCGGGAC 
AAGTTTGCTT GGGCCGTTGA CATGACCGAA ACCGATTTTA TATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

top1.2 ( XM_018092953.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948442.1 Xenopus tropicalis topoisomerase (DNA) I, gene 2 (top1.2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa43745
Clone ID Related Accession (Same CDS sequence)	XM_018092953.1
Accession Version	XM_018092953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	topoisomerase (DNA) I, gene 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACAGC TGTCGCTGCC AGTTGAAGAT CTTGTTTCTT TTCATAATCT ACCTGAGAAA 
GCAAGAGACA AAAAAAAGAA ACTAAAAAGT GAGCCAAAAC CTGAAGGGAA GGCCAAAAAG 
AAGGACAAAG GAAAAGCAGC AGTGCAGGAT GGCGAAACTC AAAGACAAAG GGGGAAAGAG 
AATAACATGC CAGTAGCTGA AGAGAGAGAA ATAAAATCGG ATAAAAGAAA AGAAAAAAAG 
AAAAAAGATA GCAAGAAAAA AAAGGAAAAG AATTCATCTG AGCACTGTGA AATTCAGCAA 
CAAGACCCTC AGACAAGTTC TGTGATTGTC AAATTAGAAC CTGATGAAGA TGGAGACTTT 
GCACCTGCTA AAAAACAAAG TAAAGGGAAA AAACGAGCAA GACAAGGGGA AGCTGGAGAA 
TTGTCAAAGT CTAAGAAAGT GAAACTAGAG GAAGATGCAG ATGGAGTTGA AACAGAAACT 
AAACCACAAA AAAAGAAAAA AATTAAAAAG GAAGAAGGTA ATAAAAAACC CAAGAAGGAA 
GAAGGTGCAA TCAAGGCAAA AAAGAAAACA CAAGAGGAGA AGGAAGCAGC AGCTGCTTTG 
AAATTAAAGA AAAAAGAAGA AGAAGAGCAG ATGCGTTGGA GATGGTGGGA GGAAGAAAAG 
TACGAAGATG GCATAAAGTG GAAGTTTCTG GAACATGAAG GTCCTGTTTT TGCTCCACCC 
TATGAGCCAT TGCCGGATAG TGTGAAATTT TACTATGATG GTAAACCCCT TCCATTAAGT 
CTACAAGCAG AAGAAGTGGC TAGTTTCTTT GCAAAAATGT TAGACCACGA GTATACAACC 
AAGGAAATCT TTCGCAATAA CTACTTTATG GATTGGAGAA AGAAAATCAA AGAGGAAAAT 
GAAAAACTGT TGCAAGAGTA TGGTTACTGT AGCATGGATC ACCATCGAGA GAGAATTGGA 
AACTTTAAGA TTGAGCCTCC AGCACTATTC CGTGGACGAG GAGACCACCC CAAACAGGGG 
ATGTTGAAGA AGCGGATACT CCCTGAAGAT ATTATAATCA ATTGTAGCAA AGACTCAAAA 
ATCCCTGAGC CACCTCCTGG GCACAAATGG AAAGAGGTGC GCCATGACAA CACTGTTACC 
TGGTTGGCAT CATGGACAGA AAATGTACAA GGATCAGTGA AGTACATCAT GCTCAATGCA 
AACTCCAAGT TGAAGGGAGA AAAAGACTGG GAGAAGTATG AAGTTGCTAG GAAGCTGAAA 
CATCATGTTG TAACAATTAG GAAGACATAC CGGGAAAATT TTAAATCCAA GGAAATGAAA 
GTGCGTCAGC GGGCCGTGGC TCTGTACTTC ATTGATAAGC TGGCTTTGAG AGCAGGTAAT 
GAAAAGGAAG AAGGAGAGAC TGCTGATACA GTTGGTTGTT GCTCCCTAAG AGTGGAGCAC 
ATAAAGCTTT ACCCAGAACT TGATGGAGAC AAGTATGTAG TTGAGTTTGA CTTCCTGGGT 
AAAGACTCTA TTCGATACTA TAACAAAGTT CCTGTGGAAA AGCAGGTCTT TAAAAACCTC 
CAGCTGTTTA TGGAGAACAA GCAGGAAGGA GATGATCTAT TTGACAGGCT GTCTACACCT 
TTGCTTAACA AGCACCTGCA GTCACTGATG GAAGGACTGA CTGCAAAGGT TTTCAGGACA 
TACAACGCAT CGATTACACT GCAGCAGCAG CTCCAGGAGC TCACAGACCC TGAAGAAAGC 
ATGCCTTCAA AGATCTTATC ATATAACAGA GCTAACAGAG CGGTTGCAAT TCTGTGCAAC 
CATCAGAGGG CACCACCAAA AACCTTTGAT CAATCTATGG CAAATCTTAA ATCTAAGATT 
GATGCGAAGA AAGAACAGAT TTTACTGGCA AAGAAAGAGC TGAAAGAAGC CAAAAAAGAG 
GCCAAAGAAA AAGAGAGTGA GAAGCTTAAT AAAGTTGTAG AGTCTAAGAA AAGAGGAGTG 
CAGAGAGCAG AGGAGCAACT CATGAGAATG GAGGTGCAGG CAACTGACCG AGAAGAGAAT 
AAACAGATTG CCTTGAGCAC CTCCAAACTA AACTATCTGG ATCCCAGGAT CTCTGTGGCC 
TGGTGCAAGA AATGGACTGT TGGACTGGAA AAAATCTACA ACAAGACGTA CCGGGACAAG 
TTTGCTTGGG CCGTTGACAT GACCGAAACC GATTTTATAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948442.1
CDS	100..2304
Translation	MTQLSLPVEDLVSFHNLPEKARDKKKKLKSEPKPEGKAKKKDKGKAAVQDGETQRQRGKENNMPVAEEREIKSDKRKEKKKKDSKKKKEKNSSEHCEIQQQDPQTSSVIVKLEPDEDGDFAPAKKQSKGKKRARQGEAGELSKSKKVKLEEDADGVETETKPQKKKKIKKEEGNKKPKKEEGAIKAKKKTQEEKEAAAALKLKKKEEEEQMRWRWWEEEKYEDGIKWKFLEHEGPVFAPPYEPLPDSVKFYYDGKPLPLSLQAEEVASFFAKMLDHEYTTKEIFRNNYFMDWRKKIKEENEKLLQEYGYCSMDHHRERIGNFKIEPPALFRGRGDHPKQGMLKKRILPEDIIINCSKDSKIPEPPPGHKWKEVRHDNTVTWLASWTENVQGSVKYIMLNANSKLKGEKDWEKYEVARKLKHHVVTIRKTYRENFKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHIKLYPELDGDKYVVEFDFLGKDSIRYYNKVPVEKQVFKNLQLFMENKQEGDDLFDRLSTPLLNKHLQSLMEGLTAKVFRTYNASITLQQQLQELTDPEESMPSKILSYNRANRAVAILCNHQRAPPKTFDQSMANLKSKIDAKKEQILLAKKELKEAKKEAKEKESEKLNKVVESKKRGVQRAEEQLMRMEVQATDREENKQIALSTSKLNYLDPRISVAWCKKWTVGLEKIYNKTYRDKFAWAVDMTETDFIF

Target ORF information:

RefSeq Version	XM_018092953.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis topoisomerase (DNA) I, gene 2 (top1.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092953.1

ORF Insert Sequence:

ATGACACAGC TGTCGCTGCC AGTTGAAGAT CTTGTTTCTT TTCATAATCT ACCTGAGAAA 
GCAAGAGACA AAAAAAAGAA ACTAAAAAGT GAGCCAAAAC CTGAAGGGAA GGCCAAAAAG 
AAGGACAAAG GAAAAGCAGC AGTGCAGGAT GGCGAAACTC AAAGACAAAG GGGGAAAGAG 
AATAACATGC CAGTAGCTGA AGAGAGAGAA ATAAAATCGG ATAAAAGAAA AGAAAAAAAG 
AAAAAAGATA GCAAGAAAAA AAAGGAAAAG AATTCATCTG AGCACTGTGA AATTCAGCAA 
CAAGACCCTC AGACAAGTTC TGTGATTGTC AAATTAGAAC CTGATGAAGA TGGAGACTTT 
GCACCTGCTA AAAAACAAAG TAAAGGGAAA AAACGAGCAA GACAAGGGGA AGCTGGAGAA 
TTGTCAAAGT CTAAGAAAGT GAAACTAGAG GAAGATGCAG ATGGAGTTGA AACAGAAACT 
AAACCACAAA AAAAGAAAAA AATTAAAAAG GAAGAAGGTA ATAAAAAACC CAAGAAGGAA 
GAAGGTGCAA TCAAGGCAAA AAAGAAAACA CAAGAGGAGA AGGAAGCAGC AGCTGCTTTG 
AAATTAAAGA AAAAAGAAGA AGAAGAGCAG ATGCGTTGGA GATGGTGGGA GGAAGAAAAG 
TACGAAGATG GCATAAAGTG GAAGTTTCTG GAACATGAAG GTCCTGTTTT TGCTCCACCC 
TATGAGCCAT TGCCGGATAG TGTGAAATTT TACTATGATG GTAAACCCCT TCCATTAAGT 
CTACAAGCAG AAGAAGTGGC TAGTTTCTTT GCAAAAATGT TAGACCACGA GTATACAACC 
AAGGAAATCT TTCGCAATAA CTACTTTATG GATTGGAGAA AGAAAATCAA AGAGGAAAAT 
GAAAAACTGT TGCAAGAGTA TGGTTACTGT AGCATGGATC ACCATCGAGA GAGAATTGGA 
AACTTTAAGA TTGAGCCTCC AGCACTATTC CGTGGACGAG GAGACCACCC CAAACAGGGG 
ATGTTGAAGA AGCGGATACT CCCTGAAGAT ATTATAATCA ATTGTAGCAA AGACTCAAAA 
ATCCCTGAGC CACCTCCTGG GCACAAATGG AAAGAGGTGC GCCATGACAA CACTGTTACC 
TGGTTGGCAT CATGGACAGA AAATGTACAA GGATCAGTGA AGTACATCAT GCTCAATGCA 
AACTCCAAGT TGAAGGGAGA AAAAGACTGG GAGAAGTATG AAGTTGCTAG GAAGCTGAAA 
CATCATGTTG TAACAATTAG GAAGACATAC CGGGAAAATT TTAAATCCAA GGAAATGAAA 
GTGCGTCAGC GGGCCGTGGC TCTGTACTTC ATTGATAAGC TGGCTTTGAG AGCAGGTAAT 
GAAAAGGAAG AAGGAGAGAC TGCTGATACA GTTGGTTGTT GCTCCCTAAG AGTGGAGCAC 
ATAAAGCTTT ACCCAGAACT TGATGGAGAC AAGTATGTAG TTGAGTTTGA CTTCCTGGGT 
AAAGACTCTA TTCGATACTA TAACAAAGTT CCTGTGGAAA AGCAGGTCTT TAAAAACCTC 
CAGCTGTTTA TGGAGAACAA GCAGGAAGGA GATGATCTAT TTGACAGGCT GTCTACACCT 
TTGCTTAACA AGCACCTGCA GTCACTGATG GAAGGACTGA CTGCAAAGGT TTTCAGGACA 
TACAACGCAT CGATTACACT GCAGCAGCAG CTCCAGGAGC TCACAGACCC TGAAGAAAGC 
ATGCCTTCAA AGATCTTATC ATATAACAGA GCTAACAGAG CGGTTGCAAT TCTGTGCAAC 
CATCAGAGGG CACCACCAAA AACCTTTGAT CAATCTATGG CAAATCTTAA ATCTAAGATT 
GATGCGAAGA AAGAACAGAT TTTACTGGCA AAGAAAGAGC TGAAAGAAGC CAAAAAAGAG 
GCCAAAGAAA AAGAGAGTGA GAAGCTTAAT AAAGTTGTAG AGTCTAAGAA AAGAGGAGTG 
CAGAGAGCAG AGGAGCAACT CATGAGAATG GAGGTGCAGG CAACTGACCG AGAAGAGAAT 
AAACAGATTG CCTTGAGCAC CTCCAAACTA AACTATCTGG ATCCCAGGAT CTCTGTGGCC 
TGGTGCAAGA AATGGACTGT TGGACTGGAA AAAATCTACA ACAAGACGTA CCGGGACAAG 
TTTGCTTGGG CCGTTGACAT GACCGAAACC GATTTTATAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

top1.2 ( XM_018092954.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948443.1 Xenopus tropicalis topoisomerase (DNA) I, gene 2 (top1.2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa43746
Clone ID Related Accession (Same CDS sequence)	XM_018092954.1
Accession Version	XM_018092954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	topoisomerase (DNA) I, gene 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGATGG AGTTGAAACA GAAACTAAAC CACAAAAAAA GGAAAAAAAT TAAAAAGGAA 
GAAGGTAATA AAAAACCCAA GAAGGAAGAA GGTGCAATCA AGGCAAAAAA GAAAACACAA 
GAGGAGAAGG AAGCAGCAGC TGCTTTGAAA TTAAAGAAAA AAGAAGAAGA AGAGCAGATG 
CGTTGGAGAT GGTGGGAGGA AGAAAAGTAC GAAGATGGCA TAAAGTGGAA GTTTCTGGAA 
CATGAAGGTC CTGTTTTTGC TCCACCCTAT GAGCCATTGC CGGATAGTGT GAAATTTTAC 
TATGATGGTA AACCCCTTCC ATTAAGTCTA CAAGCAGAAG AAGTGGCTAG TTTCTTTGCA 
AAAATGTTAG ACCACGAGTA TACAACCAAG GAAATCTTTC GCAATAACTA CTTTATGGAT 
TGGAGAAAGG AGATGACATT GGAAGAAAGG ATGGTGATTA CTGACCTGGC CAAATGTGAT 
TTTACTGAAA TGTTTACTTA CTACAAAGGA AAAATGGAGG AAAAGAAAAA TAAATCAAAA 
GAAGATAAAC TAAAAATCAA AGAGGAAAAT GAAAAACTGT TGCAAGAGTA TGGTTACTGT 
AGCATGGATC ACCATCGAGA GAGAATTGGA AACTTTAAGA TTGAGCCTCC AGCACTATTC 
CGTGGACGAG GAGACCACCC CAAACAGGGG ATGTTGAAGA AGCGGATACT CCCTGAAGAT 
ATTATAATCA ATTGTAGCAA AGACTCAAAA ATCCCTGAGC CACCTCCTGG GCACAAATGG 
AAAGAGGTGC GCCATGACAA CACTGTTACC TGGTTGGCAT CATGGACAGA AAATGTACAA 
GGATCAGTGA AGTACATCAT GCTCAATGCA AACTCCAAGT TGAAGGGAGA AAAAGACTGG 
GAGAAGTATG AAGTTGCTAG GAAGCTGAAA CATCATGTTG TAACAATTAG GAAGACATAC 
CGGGAAAATT TTAAATCCAA GGAAATGAAA GTGCGTCAGC GGGCCGTGGC TCTGTACTTC 
ATTGATAAGC TGGCTTTGAG AGCAGGTAAT GAAAAGGAAG AAGGAGAGAC TGCTGATACA 
GTTGGTTGTT GCTCCCTAAG AGTGGAGCAC ATAAAGCTTT ACCCAGAACT TGATGGAGAC 
AAGTATGTAG TTGAGTTTGA CTTCCTGGGT AAAGACTCTA TTCGATACTA TAACAAAGTT 
CCTGTGGAAA AGCAGGTCTT TAAAAACCTC CAGCTGTTTA TGGAGAACAA GCAGGAAGGA 
GATGATCTAT TTGACAGGCT GTCTACACCT TTGCTTAACA AGCACCTGCA GTCACTGATG 
GAAGGACTGA CTGCAAAGGT TTTCAGGACA TACAACGCAT CGATTACACT GCAGCAGCAG 
CTCCAGGAGC TCACAGACCC TGAAGAAAGC ATGCCTTCAA AGATCTTATC ATATAACAGA 
GCTAACAGAG CGGTTGCAAT TCTGTGCAAC CATCAGAGGG CACCACCAAA AACCTTTGAT 
CAATCTATGG CAAATCTTAA ATCTAAGATT GATGCGAAGA AAGAACAGAT TTTACTGGCA 
AAGAAAGAGC TGAAAGAAGC CAAAAAAGAG GCCAAAGAAA AAGAGAGTGA GAAGCTTAAT 
AAAGTTGTAG AGTCTAAGAA AAGAGGAGTG CAGAGAGCAG AGGAGCAACT CATGAGAATG 
GAGGTGCAGG CAACTGACCG AGAAGAGAAT AAACAGATTG CCTTGAGCAC CTCCAAACTA 
AACTATCTGG ATCCCAGGAT CTCTGTGGCC TGGTGCAAGA AATGGACTGT TGGACTGGAA 
AAAATCTACA ACAAGACGTA CCGGGACAAG TTTGCTTGGG CCGTTGACAT GACCGAAACC 
GATTTTATAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948443.1
CDS	477..2351
Translation	MQMELKQKLNHKKRKKIKKEEGNKKPKKEEGAIKAKKKTQEEKEAAAALKLKKKEEEEQMRWRWWEEEKYEDGIKWKFLEHEGPVFAPPYEPLPDSVKFYYDGKPLPLSLQAEEVASFFAKMLDHEYTTKEIFRNNYFMDWRKEMTLEERMVITDLAKCDFTEMFTYYKGKMEEKKNKSKEDKLKIKEENEKLLQEYGYCSMDHHRERIGNFKIEPPALFRGRGDHPKQGMLKKRILPEDIIINCSKDSKIPEPPPGHKWKEVRHDNTVTWLASWTENVQGSVKYIMLNANSKLKGEKDWEKYEVARKLKHHVVTIRKTYRENFKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHIKLYPELDGDKYVVEFDFLGKDSIRYYNKVPVEKQVFKNLQLFMENKQEGDDLFDRLSTPLLNKHLQSLMEGLTAKVFRTYNASITLQQQLQELTDPEESMPSKILSYNRANRAVAILCNHQRAPPKTFDQSMANLKSKIDAKKEQILLAKKELKEAKKEAKEKESEKLNKVVESKKRGVQRAEEQLMRMEVQATDREENKQIALSTSKLNYLDPRISVAWCKKWTVGLEKIYNKTYRDKFAWAVDMTETDFIF

Target ORF information:

RefSeq Version	XM_018092954.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis topoisomerase (DNA) I, gene 2 (top1.2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092954.1

ORF Insert Sequence:

ATGCAGATGG AGTTGAAACA GAAACTAAAC CACAAAAAAA GGAAAAAAAT TAAAAAGGAA 
GAAGGTAATA AAAAACCCAA GAAGGAAGAA GGTGCAATCA AGGCAAAAAA GAAAACACAA 
GAGGAGAAGG AAGCAGCAGC TGCTTTGAAA TTAAAGAAAA AAGAAGAAGA AGAGCAGATG 
CGTTGGAGAT GGTGGGAGGA AGAAAAGTAC GAAGATGGCA TAAAGTGGAA GTTTCTGGAA 
CATGAAGGTC CTGTTTTTGC TCCACCCTAT GAGCCATTGC CGGATAGTGT GAAATTTTAC 
TATGATGGTA AACCCCTTCC ATTAAGTCTA CAAGCAGAAG AAGTGGCTAG TTTCTTTGCA 
AAAATGTTAG ACCACGAGTA TACAACCAAG GAAATCTTTC GCAATAACTA CTTTATGGAT 
TGGAGAAAGG AGATGACATT GGAAGAAAGG ATGGTGATTA CTGACCTGGC CAAATGTGAT 
TTTACTGAAA TGTTTACTTA CTACAAAGGA AAAATGGAGG AAAAGAAAAA TAAATCAAAA 
GAAGATAAAC TAAAAATCAA AGAGGAAAAT GAAAAACTGT TGCAAGAGTA TGGTTACTGT 
AGCATGGATC ACCATCGAGA GAGAATTGGA AACTTTAAGA TTGAGCCTCC AGCACTATTC 
CGTGGACGAG GAGACCACCC CAAACAGGGG ATGTTGAAGA AGCGGATACT CCCTGAAGAT 
ATTATAATCA ATTGTAGCAA AGACTCAAAA ATCCCTGAGC CACCTCCTGG GCACAAATGG 
AAAGAGGTGC GCCATGACAA CACTGTTACC TGGTTGGCAT CATGGACAGA AAATGTACAA 
GGATCAGTGA AGTACATCAT GCTCAATGCA AACTCCAAGT TGAAGGGAGA AAAAGACTGG 
GAGAAGTATG AAGTTGCTAG GAAGCTGAAA CATCATGTTG TAACAATTAG GAAGACATAC 
CGGGAAAATT TTAAATCCAA GGAAATGAAA GTGCGTCAGC GGGCCGTGGC TCTGTACTTC 
ATTGATAAGC TGGCTTTGAG AGCAGGTAAT GAAAAGGAAG AAGGAGAGAC TGCTGATACA 
GTTGGTTGTT GCTCCCTAAG AGTGGAGCAC ATAAAGCTTT ACCCAGAACT TGATGGAGAC 
AAGTATGTAG TTGAGTTTGA CTTCCTGGGT AAAGACTCTA TTCGATACTA TAACAAAGTT 
CCTGTGGAAA AGCAGGTCTT TAAAAACCTC CAGCTGTTTA TGGAGAACAA GCAGGAAGGA 
GATGATCTAT TTGACAGGCT GTCTACACCT TTGCTTAACA AGCACCTGCA GTCACTGATG 
GAAGGACTGA CTGCAAAGGT TTTCAGGACA TACAACGCAT CGATTACACT GCAGCAGCAG 
CTCCAGGAGC TCACAGACCC TGAAGAAAGC ATGCCTTCAA AGATCTTATC ATATAACAGA 
GCTAACAGAG CGGTTGCAAT TCTGTGCAAC CATCAGAGGG CACCACCAAA AACCTTTGAT 
CAATCTATGG CAAATCTTAA ATCTAAGATT GATGCGAAGA AAGAACAGAT TTTACTGGCA 
AAGAAAGAGC TGAAAGAAGC CAAAAAAGAG GCCAAAGAAA AAGAGAGTGA GAAGCTTAAT 
AAAGTTGTAG AGTCTAAGAA AAGAGGAGTG CAGAGAGCAG AGGAGCAACT CATGAGAATG 
GAGGTGCAGG CAACTGACCG AGAAGAGAAT AAACAGATTG CCTTGAGCAC CTCCAAACTA 
AACTATCTGG ATCCCAGGAT CTCTGTGGCC TGGTGCAAGA AATGGACTGT TGGACTGGAA 
AAAATCTACA ACAAGACGTA CCGGGACAAG TTTGCTTGGG CCGTTGACAT GACCGAAACC 
GATTTTATAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

top1.2 ( XM_018092952.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948441.2 Xenopus tropicalis DNA topoisomerase I, gene 2 (top1.2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa55868
Clone ID Related Accession (Same CDS sequence)	XM_018092952.2
Accession Version	XM_018092952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	topoisomerase (DNA) I, gene 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACAGC TGTCGCTGCC AGTTGAAGAT CTTGTTTCTT TTCATAATCT ACCTGAGAAA 
GCAAGAGACA AAAAAAAGAA ACTAAAAAGT GAGCCAAAAC CTGAAGGGAA GGCCAAAAAG 
AAGGACAAAG GAAAAGCAGC AGTGCAGGAT GGCGAAACTC AAAGACAAAG GGGGAAAGAG 
AATAACATGC CAGTAGCTGA AGAGAGAGAA ATAAAATCGG ATAAAAGAAA AGAAAAAAAG 
AAAAAAGATA GCAAGAAAAA AAAGGAAAAG AATTCATCTG AGCACTATGA AATTCAGCAA 
CAAGACCCTC AGACAAGTTC TGTGATTGTC AAATTAGAAC CTGATGAAGA TGGAGACTTT 
GCACCTGCTA AAAAACAAAG TAAAGGGAAA AAACGAGCAA GACAAGGGGA AGCTGGAGAA 
TTGTCAAAGT CTAAGAAAGT GAAACTAGAG GAAGATGCAG ATGGAGTTGA AACAGAAACT 
AAACCACAAA AAAAGAAAAA AATTAAAAAG GAAGAAGGTA ATAAAAAACC CAAGAAGGAA 
GAAGGTGCAA TCAAGGCAAA AAAGAAAACA CAAGAGGAGA AGGAAGCAGC AGCTGCTTTG 
AAATTAAAGA AAAAAGAAGA AGAAGAGCAG ATGCGTTGGA GATGGTGGGA GGAAGAAAAG 
TACGAAGATG GCATAAAGTG GAAGTTTCTG GAACATGAAG GTCCTGTTTT TGCTCCACCC 
TATGAGCCAT TGCCGGATAG TGTGAAATTT TACTATGATG GTAAACCCCT TCCATTAAGT 
CTACAAGCAG AAGAAGTGGC TAGTTTCTTT GCAAAAATGT TAGACCACGA GTATACAACC 
AAGGAAATCT TTCGCAATAA CTACTTTATG GATTGGAGAA AGGAGATGAC ATTGGAAGAA 
AGGATGGTGA TTACTGACCT GGCCAAATGT GATTTTACTG AAATGTTTAC TTACTACAAA 
GGAAAAATGG AGGAAAAGAA AAATAAATCA AAAGAAGATA AACTAAAAAT CAAAGAGGAA 
AATGAAAAAC TGTTGCAAGA GTATGGTTAC TGTAGCATGG ATCACCATCG AGAGAGAATT 
GGAAACTTTA AGATTGAGCC TCCAGCACTA TTCCGTGGAC GAGGAGACCA CCCCAAACAG 
GGGATGTTGA AGAAGCGGAT ACTCCCTGAA GATATTATAA TCAATTGTAG CAAAGACTCA 
AAAATCCCTG AGCCACCTCC TGGGCACAAA TGGAAAGAGG TGCGCCATGA CAACACTGTT 
ACCTGGTTGG CATCATGGAC AGAAAATGTA CAAGGATCAG TGAAGTACAT CATGCTCAAT 
GCAAACTCCA AGTTGAAGGG AGAAAAAGAC TGGGAGAAGT ATGAAGTTGC TAGGAAGCTG 
AAACATCATG TTGTAACAAT TAGGAAGACA TACCGGGAAA ATTTTAAATC CAAGGAAATG 
AAAGTGCGTC AGCGGGCCGT GGCTCTGTAC TTCATTGATA AGCTGGCTTT GAGAGCAGGT 
AATGAAAAGG AAGAAGGAGA GACTGCTGAT ACAGTTGGTT GTTGCTCCCT AAGAGTGGAG 
CACATAAAGC TTTACCCAGA ACTTGATGGA GACAAGTATG TAGTTGAGTT TGACTTCCTG 
GGTAAAGACT CTATTCGATA CTATAACAAA GTTCCTGTGG AAAAGCAGGT CTTTAAAAAC 
CTCCAGCTGT TTATGGAGAA CAAGCAGGAA GGAGATGATC TATTTGACAG GCTGTCTACA 
CCTTTGCTTA ACAAGCACCT GCAGTCACTG ATGGAAGGAC TGACTGCAAA GGTTTTCAGG 
ACATACAACG CATCGATTAC ACTGCAGCAG CAGCTCCAGG AGCTCACAGA CCCTGAAGAA 
AGCATGCCTT CAAAGATCTT ATCATATAAC AGAGCTAACA GAGCGGTTGC AATTCTGTGC 
AACCATCAGA GGGCACCACC AAAAACCTTT GATCAATCTA TGGCAAATCT TAAATCTAAG 
ATTGATGCGA AGAAAGAACA GATTTTACTG GCAAAGAAAG AGCTGAAAGA AGCCAAAAAA 
GAGGCCAAAG AAAAAGAGAG TGAGAAGCTT AATAAAGTTG TAGAGTCTAA GAAAAGAGGA 
GTGCAGAGAG CAGAGGAGCA ACTCATGAGA ATGGAGGTGC AGGCAACTGA CCGAGAAGAG 
AATAAACAGA TTGCCTTGAG CACCTCCAAA CTAAACTATC TGGATCCCAG GATCTCTGTG 
GCCTGGTGCA AGAAATGGAC TGTTGGACTG GAAAAAATCT ACAACAAGAC GTACCGGGAC 
AAGTTTGCTT GGGCCGTTGA CATGACCGAA ACCGATTTTA TATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948441.2
CDS	85..2412
Translation	MTQLSLPVEDLVSFHNLPEKARDKKKKLKSEPKPEGKAKKKDKGKAAVQDGETQRQRGKENNMPVAEEREIKSDKRKEKKKKDSKKKKEKNSSEHYEIQQQDPQTSSVIVKLEPDEDGDFAPAKKQSKGKKRARQGEAGELSKSKKVKLEEDADGVETETKPQKKKKIKKEEGNKKPKKEEGAIKAKKKTQEEKEAAAALKLKKKEEEEQMRWRWWEEEKYEDGIKWKFLEHEGPVFAPPYEPLPDSVKFYYDGKPLPLSLQAEEVASFFAKMLDHEYTTKEIFRNNYFMDWRKEMTLEERMVITDLAKCDFTEMFTYYKGKMEEKKNKSKEDKLKIKEENEKLLQEYGYCSMDHHRERIGNFKIEPPALFRGRGDHPKQGMLKKRILPEDIIINCSKDSKIPEPPPGHKWKEVRHDNTVTWLASWTENVQGSVKYIMLNANSKLKGEKDWEKYEVARKLKHHVVTIRKTYRENFKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHIKLYPELDGDKYVVEFDFLGKDSIRYYNKVPVEKQVFKNLQLFMENKQEGDDLFDRLSTPLLNKHLQSLMEGLTAKVFRTYNASITLQQQLQELTDPEESMPSKILSYNRANRAVAILCNHQRAPPKTFDQSMANLKSKIDAKKEQILLAKKELKEAKKEAKEKESEKLNKVVESKKRGVQRAEEQLMRMEVQATDREENKQIALSTSKLNYLDPRISVAWCKKWTVGLEKIYNKTYRDKFAWAVDMTETDFIF

Target ORF information:

RefSeq Version	XM_018092952.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DNA topoisomerase I, gene 2 (top1.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092952.2

ORF Insert Sequence:

ATGACACAGC TGTCGCTGCC AGTTGAAGAT CTTGTTTCTT TTCATAATCT ACCTGAGAAA 
GCAAGAGACA AAAAAAAGAA ACTAAAAAGT GAGCCAAAAC CTGAAGGGAA GGCCAAAAAG 
AAGGACAAAG GAAAAGCAGC AGTGCAGGAT GGCGAAACTC AAAGACAAAG GGGGAAAGAG 
AATAACATGC CAGTAGCTGA AGAGAGAGAA ATAAAATCGG ATAAAAGAAA AGAAAAAAAG 
AAAAAAGATA GCAAGAAAAA AAAGGAAAAG AATTCATCTG AGCACTATGA AATTCAGCAA 
CAAGACCCTC AGACAAGTTC TGTGATTGTC AAATTAGAAC CTGATGAAGA TGGAGACTTT 
GCACCTGCTA AAAAACAAAG TAAAGGGAAA AAACGAGCAA GACAAGGGGA AGCTGGAGAA 
TTGTCAAAGT CTAAGAAAGT GAAACTAGAG GAAGATGCAG ATGGAGTTGA AACAGAAACT 
AAACCACAAA AAAAGAAAAA AATTAAAAAG GAAGAAGGTA ATAAAAAACC CAAGAAGGAA 
GAAGGTGCAA TCAAGGCAAA AAAGAAAACA CAAGAGGAGA AGGAAGCAGC AGCTGCTTTG 
AAATTAAAGA AAAAAGAAGA AGAAGAGCAG ATGCGTTGGA GATGGTGGGA GGAAGAAAAG 
TACGAAGATG GCATAAAGTG GAAGTTTCTG GAACATGAAG GTCCTGTTTT TGCTCCACCC 
TATGAGCCAT TGCCGGATAG TGTGAAATTT TACTATGATG GTAAACCCCT TCCATTAAGT 
CTACAAGCAG AAGAAGTGGC TAGTTTCTTT GCAAAAATGT TAGACCACGA GTATACAACC 
AAGGAAATCT TTCGCAATAA CTACTTTATG GATTGGAGAA AGGAGATGAC ATTGGAAGAA 
AGGATGGTGA TTACTGACCT GGCCAAATGT GATTTTACTG AAATGTTTAC TTACTACAAA 
GGAAAAATGG AGGAAAAGAA AAATAAATCA AAAGAAGATA AACTAAAAAT CAAAGAGGAA 
AATGAAAAAC TGTTGCAAGA GTATGGTTAC TGTAGCATGG ATCACCATCG AGAGAGAATT 
GGAAACTTTA AGATTGAGCC TCCAGCACTA TTCCGTGGAC GAGGAGACCA CCCCAAACAG 
GGGATGTTGA AGAAGCGGAT ACTCCCTGAA GATATTATAA TCAATTGTAG CAAAGACTCA 
AAAATCCCTG AGCCACCTCC TGGGCACAAA TGGAAAGAGG TGCGCCATGA CAACACTGTT 
ACCTGGTTGG CATCATGGAC AGAAAATGTA CAAGGATCAG TGAAGTACAT CATGCTCAAT 
GCAAACTCCA AGTTGAAGGG AGAAAAAGAC TGGGAGAAGT ATGAAGTTGC TAGGAAGCTG 
AAACATCATG TTGTAACAAT TAGGAAGACA TACCGGGAAA ATTTTAAATC CAAGGAAATG 
AAAGTGCGTC AGCGGGCCGT GGCTCTGTAC TTCATTGATA AGCTGGCTTT GAGAGCAGGT 
AATGAAAAGG AAGAAGGAGA GACTGCTGAT ACAGTTGGTT GTTGCTCCCT AAGAGTGGAG 
CACATAAAGC TTTACCCAGA ACTTGATGGA GACAAGTATG TAGTTGAGTT TGACTTCCTG 
GGTAAAGACT CTATTCGATA CTATAACAAA GTTCCTGTGG AAAAGCAGGT CTTTAAAAAC 
CTCCAGCTGT TTATGGAGAA CAAGCAGGAA GGAGATGATC TATTTGACAG GCTGTCTACA 
CCTTTGCTTA ACAAGCACCT GCAGTCACTG ATGGAAGGAC TGACTGCAAA GGTTTTCAGG 
ACATACAACG CATCGATTAC ACTGCAGCAG CAGCTCCAGG AGCTCACAGA CCCTGAAGAA 
AGCATGCCTT CAAAGATCTT ATCATATAAC AGAGCTAACA GAGCGGTTGC AATTCTGTGC 
AACCATCAGA GGGCACCACC AAAAACCTTT GATCAATCTA TGGCAAATCT TAAATCTAAG 
ATTGATGCGA AGAAAGAACA GATTTTACTG GCAAAGAAAG AGCTGAAAGA AGCCAAAAAA 
GAGGCCAAAG AAAAAGAGAG TGAGAAGCTT AATAAAGTTG TAGAGTCTAA GAAAAGAGGA 
GTGCAGAGAG CAGAGGAGCA ACTCATGAGA ATGGAGGTGC AGGCAACTGA CCGAGAAGAG 
AATAAACAGA TTGCCTTGAG CACCTCCAAA CTAAACTATC TGGATCCCAG GATCTCTGTG 
GCCTGGTGCA AGAAATGGAC TGTTGGACTG GAAAAAATCT ACAACAAGAC GTACCGGGAC 
AAGTTTGCTT GGGCCGTTGA CATGACCGAA ACCGATTTTA TATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

top1.2 ( XM_018092953.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948442.2 Xenopus tropicalis DNA topoisomerase I, gene 2 (top1.2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa55869
Clone ID Related Accession (Same CDS sequence)	XM_018092953.2
Accession Version	XM_018092953.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	topoisomerase (DNA) I, gene 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACAGC TGTCGCTGCC AGTTGAAGAT CTTGTTTCTT TTCATAATCT ACCTGAGAAA 
GCAAGAGACA AAAAAAAGAA ACTAAAAAGT GAGCCAAAAC CTGAAGGGAA GGCCAAAAAG 
AAGGACAAAG GAAAAGCAGC AGTGCAGGAT GGCGAAACTC AAAGACAAAG GGGGAAAGAG 
AATAACATGC CAGTAGCTGA AGAGAGAGAA ATAAAATCGG ATAAAAGAAA AGAAAAAAAG 
AAAAAAGATA GCAAGAAAAA AAAGGAAAAG AATTCATCTG AGCACTATGA AATTCAGCAA 
CAAGACCCTC AGACAAGTTC TGTGATTGTC AAATTAGAAC CTGATGAAGA TGGAGACTTT 
GCACCTGCTA AAAAACAAAG TAAAGGGAAA AAACGAGCAA GACAAGGGGA AGCTGGAGAA 
TTGTCAAAGT CTAAGAAAGT GAAACTAGAG GAAGATGCAG ATGGAGTTGA AACAGAAACT 
AAACCACAAA AAAAGAAAAA AATTAAAAAG GAAGAAGGTA ATAAAAAACC CAAGAAGGAA 
GAAGGTGCAA TCAAGGCAAA AAAGAAAACA CAAGAGGAGA AGGAAGCAGC AGCTGCTTTG 
AAATTAAAGA AAAAAGAAGA AGAAGAGCAG ATGCGTTGGA GATGGTGGGA GGAAGAAAAG 
TACGAAGATG GCATAAAGTG GAAGTTTCTG GAACATGAAG GTCCTGTTTT TGCTCCACCC 
TATGAGCCAT TGCCGGATAG TGTGAAATTT TACTATGATG GTAAACCCCT TCCATTAAGT 
CTACAAGCAG AAGAAGTGGC TAGTTTCTTT GCAAAAATGT TAGACCACGA GTATACAACC 
AAGGAAATCT TTCGCAATAA CTACTTTATG GATTGGAGAA AGAAAATCAA AGAGGAAAAT 
GAAAAACTGT TGCAAGAGTA TGGTTACTGT AGCATGGATC ACCATCGAGA GAGAATTGGA 
AACTTTAAGA TTGAGCCTCC AGCACTATTC CGTGGACGAG GAGACCACCC CAAACAGGGG 
ATGTTGAAGA AGCGGATACT CCCTGAAGAT ATTATAATCA ATTGTAGCAA AGACTCAAAA 
ATCCCTGAGC CACCTCCTGG GCACAAATGG AAAGAGGTGC GCCATGACAA CACTGTTACC 
TGGTTGGCAT CATGGACAGA AAATGTACAA GGATCAGTGA AGTACATCAT GCTCAATGCA 
AACTCCAAGT TGAAGGGAGA AAAAGACTGG GAGAAGTATG AAGTTGCTAG GAAGCTGAAA 
CATCATGTTG TAACAATTAG GAAGACATAC CGGGAAAATT TTAAATCCAA GGAAATGAAA 
GTGCGTCAGC GGGCCGTGGC TCTGTACTTC ATTGATAAGC TGGCTTTGAG AGCAGGTAAT 
GAAAAGGAAG AAGGAGAGAC TGCTGATACA GTTGGTTGTT GCTCCCTAAG AGTGGAGCAC 
ATAAAGCTTT ACCCAGAACT TGATGGAGAC AAGTATGTAG TTGAGTTTGA CTTCCTGGGT 
AAAGACTCTA TTCGATACTA TAACAAAGTT CCTGTGGAAA AGCAGGTCTT TAAAAACCTC 
CAGCTGTTTA TGGAGAACAA GCAGGAAGGA GATGATCTAT TTGACAGGCT GTCTACACCT 
TTGCTTAACA AGCACCTGCA GTCACTGATG GAAGGACTGA CTGCAAAGGT TTTCAGGACA 
TACAACGCAT CGATTACACT GCAGCAGCAG CTCCAGGAGC TCACAGACCC TGAAGAAAGC 
ATGCCTTCAA AGATCTTATC ATATAACAGA GCTAACAGAG CGGTTGCAAT TCTGTGCAAC 
CATCAGAGGG CACCACCAAA AACCTTTGAT CAATCTATGG CAAATCTTAA ATCTAAGATT 
GATGCGAAGA AAGAACAGAT TTTACTGGCA AAGAAAGAGC TGAAAGAAGC CAAAAAAGAG 
GCCAAAGAAA AAGAGAGTGA GAAGCTTAAT AAAGTTGTAG AGTCTAAGAA AAGAGGAGTG 
CAGAGAGCAG AGGAGCAACT CATGAGAATG GAGGTGCAGG CAACTGACCG AGAAGAGAAT 
AAACAGATTG CCTTGAGCAC CTCCAAACTA AACTATCTGG ATCCCAGGAT CTCTGTGGCC 
TGGTGCAAGA AATGGACTGT TGGACTGGAA AAAATCTACA ACAAGACGTA CCGGGACAAG 
TTTGCTTGGG CCGTTGACAT GACCGAAACC GATTTTATAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948442.2
CDS	85..2289
Translation	MTQLSLPVEDLVSFHNLPEKARDKKKKLKSEPKPEGKAKKKDKGKAAVQDGETQRQRGKENNMPVAEEREIKSDKRKEKKKKDSKKKKEKNSSEHYEIQQQDPQTSSVIVKLEPDEDGDFAPAKKQSKGKKRARQGEAGELSKSKKVKLEEDADGVETETKPQKKKKIKKEEGNKKPKKEEGAIKAKKKTQEEKEAAAALKLKKKEEEEQMRWRWWEEEKYEDGIKWKFLEHEGPVFAPPYEPLPDSVKFYYDGKPLPLSLQAEEVASFFAKMLDHEYTTKEIFRNNYFMDWRKKIKEENEKLLQEYGYCSMDHHRERIGNFKIEPPALFRGRGDHPKQGMLKKRILPEDIIINCSKDSKIPEPPPGHKWKEVRHDNTVTWLASWTENVQGSVKYIMLNANSKLKGEKDWEKYEVARKLKHHVVTIRKTYRENFKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHIKLYPELDGDKYVVEFDFLGKDSIRYYNKVPVEKQVFKNLQLFMENKQEGDDLFDRLSTPLLNKHLQSLMEGLTAKVFRTYNASITLQQQLQELTDPEESMPSKILSYNRANRAVAILCNHQRAPPKTFDQSMANLKSKIDAKKEQILLAKKELKEAKKEAKEKESEKLNKVVESKKRGVQRAEEQLMRMEVQATDREENKQIALSTSKLNYLDPRISVAWCKKWTVGLEKIYNKTYRDKFAWAVDMTETDFIF

Target ORF information:

RefSeq Version	XM_018092953.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DNA topoisomerase I, gene 2 (top1.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092953.2

ORF Insert Sequence:

ATGACACAGC TGTCGCTGCC AGTTGAAGAT CTTGTTTCTT TTCATAATCT ACCTGAGAAA 
GCAAGAGACA AAAAAAAGAA ACTAAAAAGT GAGCCAAAAC CTGAAGGGAA GGCCAAAAAG 
AAGGACAAAG GAAAAGCAGC AGTGCAGGAT GGCGAAACTC AAAGACAAAG GGGGAAAGAG 
AATAACATGC CAGTAGCTGA AGAGAGAGAA ATAAAATCGG ATAAAAGAAA AGAAAAAAAG 
AAAAAAGATA GCAAGAAAAA AAAGGAAAAG AATTCATCTG AGCACTATGA AATTCAGCAA 
CAAGACCCTC AGACAAGTTC TGTGATTGTC AAATTAGAAC CTGATGAAGA TGGAGACTTT 
GCACCTGCTA AAAAACAAAG TAAAGGGAAA AAACGAGCAA GACAAGGGGA AGCTGGAGAA 
TTGTCAAAGT CTAAGAAAGT GAAACTAGAG GAAGATGCAG ATGGAGTTGA AACAGAAACT 
AAACCACAAA AAAAGAAAAA AATTAAAAAG GAAGAAGGTA ATAAAAAACC CAAGAAGGAA 
GAAGGTGCAA TCAAGGCAAA AAAGAAAACA CAAGAGGAGA AGGAAGCAGC AGCTGCTTTG 
AAATTAAAGA AAAAAGAAGA AGAAGAGCAG ATGCGTTGGA GATGGTGGGA GGAAGAAAAG 
TACGAAGATG GCATAAAGTG GAAGTTTCTG GAACATGAAG GTCCTGTTTT TGCTCCACCC 
TATGAGCCAT TGCCGGATAG TGTGAAATTT TACTATGATG GTAAACCCCT TCCATTAAGT 
CTACAAGCAG AAGAAGTGGC TAGTTTCTTT GCAAAAATGT TAGACCACGA GTATACAACC 
AAGGAAATCT TTCGCAATAA CTACTTTATG GATTGGAGAA AGAAAATCAA AGAGGAAAAT 
GAAAAACTGT TGCAAGAGTA TGGTTACTGT AGCATGGATC ACCATCGAGA GAGAATTGGA 
AACTTTAAGA TTGAGCCTCC AGCACTATTC CGTGGACGAG GAGACCACCC CAAACAGGGG 
ATGTTGAAGA AGCGGATACT CCCTGAAGAT ATTATAATCA ATTGTAGCAA AGACTCAAAA 
ATCCCTGAGC CACCTCCTGG GCACAAATGG AAAGAGGTGC GCCATGACAA CACTGTTACC 
TGGTTGGCAT CATGGACAGA AAATGTACAA GGATCAGTGA AGTACATCAT GCTCAATGCA 
AACTCCAAGT TGAAGGGAGA AAAAGACTGG GAGAAGTATG AAGTTGCTAG GAAGCTGAAA 
CATCATGTTG TAACAATTAG GAAGACATAC CGGGAAAATT TTAAATCCAA GGAAATGAAA 
GTGCGTCAGC GGGCCGTGGC TCTGTACTTC ATTGATAAGC TGGCTTTGAG AGCAGGTAAT 
GAAAAGGAAG AAGGAGAGAC TGCTGATACA GTTGGTTGTT GCTCCCTAAG AGTGGAGCAC 
ATAAAGCTTT ACCCAGAACT TGATGGAGAC AAGTATGTAG TTGAGTTTGA CTTCCTGGGT 
AAAGACTCTA TTCGATACTA TAACAAAGTT CCTGTGGAAA AGCAGGTCTT TAAAAACCTC 
CAGCTGTTTA TGGAGAACAA GCAGGAAGGA GATGATCTAT TTGACAGGCT GTCTACACCT 
TTGCTTAACA AGCACCTGCA GTCACTGATG GAAGGACTGA CTGCAAAGGT TTTCAGGACA 
TACAACGCAT CGATTACACT GCAGCAGCAG CTCCAGGAGC TCACAGACCC TGAAGAAAGC 
ATGCCTTCAA AGATCTTATC ATATAACAGA GCTAACAGAG CGGTTGCAAT TCTGTGCAAC 
CATCAGAGGG CACCACCAAA AACCTTTGAT CAATCTATGG CAAATCTTAA ATCTAAGATT 
GATGCGAAGA AAGAACAGAT TTTACTGGCA AAGAAAGAGC TGAAAGAAGC CAAAAAAGAG 
GCCAAAGAAA AAGAGAGTGA GAAGCTTAAT AAAGTTGTAG AGTCTAAGAA AAGAGGAGTG 
CAGAGAGCAG AGGAGCAACT CATGAGAATG GAGGTGCAGG CAACTGACCG AGAAGAGAAT 
AAACAGATTG CCTTGAGCAC CTCCAAACTA AACTATCTGG ATCCCAGGAT CTCTGTGGCC 
TGGTGCAAGA AATGGACTGT TGGACTGGAA AAAATCTACA ACAAGACGTA CCGGGACAAG 
TTTGCTTGGG CCGTTGACAT GACCGAAACC GATTTTATAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

top1.2 ( XM_031899929.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755789.1 Xenopus tropicalis DNA topoisomerase I, gene 2 (top1.2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa55870
Clone ID Related Accession (Same CDS sequence)	XM_031899929.1
Accession Version	XM_031899929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	topoisomerase (DNA) I, gene 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACAGC TGTCGCTGCC AGTTGAAGAT CTTGTTTCTT TTCATAATCT ACCTGAGAAA 
GCAAGAGACA AAAAAAAGAA ACTAAAAAGT GAGCCAAAAC CTGAAGGGAA GGCCAAAAAG 
AAGGACAAAG GAAAAGCAGC AGTGCAGGAT GGCGAAACTC AAAGACAAAG GGGGAAAGAG 
AATAACATGC CAGTAGCTGA AGAGAGAGAA ATAAAATCGG ATAAAAGAAA AGAAAAAAAG 
AAAAAAGATA GCAAGAAAAA AAAGGAAAAG AATTCATCTG AGCACTATGA AATTCAGCAA 
CAAGACCCTC AGACAAGTTC TGTGATTGTC AAATTAGAAC CTGATGAAGA TGGAGACTTT 
GCACCTGCTA AAAAACAAAG TAAAGGGAAA AAACGAGCAA GACAAGGGGA AGCTGGAGAA 
TTGTCAAAGT CTAAGAAAGT GAAACTAGAG GAAGATGCAG ATGGAGTTGA AACAGAAACT 
AAACCACAAA AAAAGAAAAA AATTAAAAAG GAAGAAGGTA ATAAAAAACC CAAGAAGGAA 
GAAGGTGCAA TCAAGGCAAA AAAGAAAACA CAAGAGGAGA AGGAAGCAGC AGCTGCTTTG 
AAATTAAAGA AAAAAGAAGA AGAAGAGCAG ATGCGTTGGA GATGGTGGGA GGAAGAAAAG 
TACGAAGATG GCATAAAGTG GAAGTTTCTG GAACATGAAG GTCCTGTTTT TGCTCCACCC 
TATGAGCCAT TGCCGGATAG TGTGAAATTT TACTATGATG GTAAACCCCT TCCATTAAGT 
CTACAAGCAG AAGAAGTGGC TAGTTTCTTT GCAAAAATGT TAGACCACGA GTATACAACC 
AAGGAAATCT TTCGCAATAA CTACTTTATG GATTGGAGAA AGGAGATGAC ATTGGAAGAA 
AGGATGGTGA TTACTGACCT GGCCAAATGT GATTTTACTG AAATGTTTAC TTACTACAAA 
GGAAAAATGG AGGAAAAGAA AAATAAATCA AAAGAAGATA AACTAAAAAT CAAAGAGGAA 
AATGAAAAAC TGTTGCAAGA GTATGGTTAC TGTAGCATGG ATCACCATCG AGAGAGAATT 
GGAAACTTTA AGATTGAGCC TCCAGCACTA TTCCGTGGAC GAGGAGACCA CCCCAAACAG 
GGGATGTTGA AGAAGCGGAT ACTCCCTGAA GATATTATAA TCAATTGTAG CAAAGACTCA 
AAAATCCCTG AGCCACCTCC TGGGCACAAA TGGAAAGAGG TGCGCCATGA CAACACTGTT 
ACCTGGTTGG CATCATGGAC AGAAAATGTA CAAGGATCAG TGAAGTACAT CATGCTCAAT 
GCAAACTCCA AGTTGAAGCT GGCTTTGAGA GCAGGTAATG AAAAGGAAGA AGGAGAGACT 
GCTGATACAG TTGGTTGTTG CTCCCTAAGA GTGGAGCACA TAAAGCTTTA CCCAGAACTT 
GATGGAGACA AGTATGTAGT TGAGTTTGAC TTCCTGGGTA AAGACTCTAT TCGATACTAT 
AACAAAGTTC CTGTGGAAAA GCAGGTCTTT AAAAACCTCC AGCTGTTTAT GGAGAACAAG 
CAGGAAGGAG ATGATCTATT TGACAGGCTG TCTACACCTT TGCTTAACAA GCACCTGCAG 
TCACTGATGG AAGGACTGAC TGCAAAGGTT TTCAGGACAT ACAACGCATC GATTACACTG 
CAGCAGCAGC TCCAGGAGCT CACAGACCCT GAAGAAAGCA TGCCTTCAAA GATCTTATCA 
TATAACAGAG CTAACAGAGC GGTTGCAATT CTGTGCAACC ATCAGAGGGC ACCACCAAAA 
ACCTTTGATC AATCTATGGC AAATCTTAAA TCTAAGATTG ATGCGAAGAA AGAACAGATT 
TTACTGGCAA AGAAAGAGCT GAAAGAAGCC AAAAAAGAGG CCAAAGAAAA AGAGAGTGAG 
AAGCTTAATA AAGTTGTAGA GTCTAAGAAA AGAGGAGTGC AGAGAGCAGA GGAGCAACTC 
ATGAGAATGG AGGTGCAGGC AACTGACCGA GAAGAGAATA AACAGATTGC CTTGAGCACC 
TCCAAACTAA ACTATCTGGA TCCCAGGATC TCTGTGGCCT GGTGCAAGAA ATGGACTGTT 
GGACTGGAAA AAATCTACAA CAAGACGTAC CGGGACAAGT TTGCTTGGGC CGTTGACATG 
ACCGAAACCG ATTTTATATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755789.1
CDS	85..2268
Translation	MTQLSLPVEDLVSFHNLPEKARDKKKKLKSEPKPEGKAKKKDKGKAAVQDGETQRQRGKENNMPVAEEREIKSDKRKEKKKKDSKKKKEKNSSEHYEIQQQDPQTSSVIVKLEPDEDGDFAPAKKQSKGKKRARQGEAGELSKSKKVKLEEDADGVETETKPQKKKKIKKEEGNKKPKKEEGAIKAKKKTQEEKEAAAALKLKKKEEEEQMRWRWWEEEKYEDGIKWKFLEHEGPVFAPPYEPLPDSVKFYYDGKPLPLSLQAEEVASFFAKMLDHEYTTKEIFRNNYFMDWRKEMTLEERMVITDLAKCDFTEMFTYYKGKMEEKKNKSKEDKLKIKEENEKLLQEYGYCSMDHHRERIGNFKIEPPALFRGRGDHPKQGMLKKRILPEDIIINCSKDSKIPEPPPGHKWKEVRHDNTVTWLASWTENVQGSVKYIMLNANSKLKLALRAGNEKEEGETADTVGCCSLRVEHIKLYPELDGDKYVVEFDFLGKDSIRYYNKVPVEKQVFKNLQLFMENKQEGDDLFDRLSTPLLNKHLQSLMEGLTAKVFRTYNASITLQQQLQELTDPEESMPSKILSYNRANRAVAILCNHQRAPPKTFDQSMANLKSKIDAKKEQILLAKKELKEAKKEAKEKESEKLNKVVESKKRGVQRAEEQLMRMEVQATDREENKQIALSTSKLNYLDPRISVAWCKKWTVGLEKIYNKTYRDKFAWAVDMTETDFIF

Target ORF information:

RefSeq Version	XM_031899929.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DNA topoisomerase I, gene 2 (top1.2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899929.1

ORF Insert Sequence:

ATGACACAGC TGTCGCTGCC AGTTGAAGAT CTTGTTTCTT TTCATAATCT ACCTGAGAAA 
GCAAGAGACA AAAAAAAGAA ACTAAAAAGT GAGCCAAAAC CTGAAGGGAA GGCCAAAAAG 
AAGGACAAAG GAAAAGCAGC AGTGCAGGAT GGCGAAACTC AAAGACAAAG GGGGAAAGAG 
AATAACATGC CAGTAGCTGA AGAGAGAGAA ATAAAATCGG ATAAAAGAAA AGAAAAAAAG 
AAAAAAGATA GCAAGAAAAA AAAGGAAAAG AATTCATCTG AGCACTATGA AATTCAGCAA 
CAAGACCCTC AGACAAGTTC TGTGATTGTC AAATTAGAAC CTGATGAAGA TGGAGACTTT 
GCACCTGCTA AAAAACAAAG TAAAGGGAAA AAACGAGCAA GACAAGGGGA AGCTGGAGAA 
TTGTCAAAGT CTAAGAAAGT GAAACTAGAG GAAGATGCAG ATGGAGTTGA AACAGAAACT 
AAACCACAAA AAAAGAAAAA AATTAAAAAG GAAGAAGGTA ATAAAAAACC CAAGAAGGAA 
GAAGGTGCAA TCAAGGCAAA AAAGAAAACA CAAGAGGAGA AGGAAGCAGC AGCTGCTTTG 
AAATTAAAGA AAAAAGAAGA AGAAGAGCAG ATGCGTTGGA GATGGTGGGA GGAAGAAAAG 
TACGAAGATG GCATAAAGTG GAAGTTTCTG GAACATGAAG GTCCTGTTTT TGCTCCACCC 
TATGAGCCAT TGCCGGATAG TGTGAAATTT TACTATGATG GTAAACCCCT TCCATTAAGT 
CTACAAGCAG AAGAAGTGGC TAGTTTCTTT GCAAAAATGT TAGACCACGA GTATACAACC 
AAGGAAATCT TTCGCAATAA CTACTTTATG GATTGGAGAA AGGAGATGAC ATTGGAAGAA 
AGGATGGTGA TTACTGACCT GGCCAAATGT GATTTTACTG AAATGTTTAC TTACTACAAA 
GGAAAAATGG AGGAAAAGAA AAATAAATCA AAAGAAGATA AACTAAAAAT CAAAGAGGAA 
AATGAAAAAC TGTTGCAAGA GTATGGTTAC TGTAGCATGG ATCACCATCG AGAGAGAATT 
GGAAACTTTA AGATTGAGCC TCCAGCACTA TTCCGTGGAC GAGGAGACCA CCCCAAACAG 
GGGATGTTGA AGAAGCGGAT ACTCCCTGAA GATATTATAA TCAATTGTAG CAAAGACTCA 
AAAATCCCTG AGCCACCTCC TGGGCACAAA TGGAAAGAGG TGCGCCATGA CAACACTGTT 
ACCTGGTTGG CATCATGGAC AGAAAATGTA CAAGGATCAG TGAAGTACAT CATGCTCAAT 
GCAAACTCCA AGTTGAAGCT GGCTTTGAGA GCAGGTAATG AAAAGGAAGA AGGAGAGACT 
GCTGATACAG TTGGTTGTTG CTCCCTAAGA GTGGAGCACA TAAAGCTTTA CCCAGAACTT 
GATGGAGACA AGTATGTAGT TGAGTTTGAC TTCCTGGGTA AAGACTCTAT TCGATACTAT 
AACAAAGTTC CTGTGGAAAA GCAGGTCTTT AAAAACCTCC AGCTGTTTAT GGAGAACAAG 
CAGGAAGGAG ATGATCTATT TGACAGGCTG TCTACACCTT TGCTTAACAA GCACCTGCAG 
TCACTGATGG AAGGACTGAC TGCAAAGGTT TTCAGGACAT ACAACGCATC GATTACACTG 
CAGCAGCAGC TCCAGGAGCT CACAGACCCT GAAGAAAGCA TGCCTTCAAA GATCTTATCA 
TATAACAGAG CTAACAGAGC GGTTGCAATT CTGTGCAACC ATCAGAGGGC ACCACCAAAA 
ACCTTTGATC AATCTATGGC AAATCTTAAA TCTAAGATTG ATGCGAAGAA AGAACAGATT 
TTACTGGCAA AGAAAGAGCT GAAAGAAGCC AAAAAAGAGG CCAAAGAAAA AGAGAGTGAG 
AAGCTTAATA AAGTTGTAGA GTCTAAGAAA AGAGGAGTGC AGAGAGCAGA GGAGCAACTC 
ATGAGAATGG AGGTGCAGGC AACTGACCGA GAAGAGAATA AACAGATTGC CTTGAGCACC 
TCCAAACTAA ACTATCTGGA TCCCAGGATC TCTGTGGCCT GGTGCAAGAA ATGGACTGTT 
GGACTGGAAA AAATCTACAA CAAGACGTAC CGGGACAAGT TTGCTTGGGC CGTTGACATG 
ACCGAAACCG ATTTTATATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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top1.2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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