magi1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012961759.2	XP_012817213.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
XM_012961760.2	XP_012817214.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
XM_012961762.2	XP_012817216.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
XM_031901230.1	XP_031757090.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
XM_031901231.1	XP_031757091.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
XM_031901232.1	XP_031757092.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X6
XM_031901227.1	XP_031757087.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
XM_031901229.1	XP_031757089.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
XM_031901236.1	XP_031757096.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X9
XM_031901237.1	XP_031757097.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X10
XM_012961761.2	XP_012817215.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
XM_012961766.2	XP_012817220.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X8
XM_031901238.1	XP_031757098.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X11
XM_031901239.1	XP_031757099.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X12
XM_031901241.1	XP_031757101.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X14
XM_012961763.2	XP_012817217.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
XM_012961765.2	XP_012817219.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X7
XM_031901240.1	XP_031757100.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X13
XM_018089383.1	XP_017944872.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
XM_012961764.2	XP_012817218.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X6
XM_031901228.1	XP_031757088.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
XM_031901233.1	XP_031757093.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X7
XM_031901235.1	XP_031757095.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa33038	XM_012961759.2 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OXa33039	XM_012961760.2 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OXa33040	XM_012961761.2 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OXa33041	XM_012961762.2 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OXa33042	XM_018089383.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OXa33042	XM_012961763.2 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OXa33043	XM_012961764.2 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$713.30 $1019.00
OXa33045	XM_012961766.2 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OXa33046	XM_031901239.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa57624	XM_031901227.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa57625	XM_031901228.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa57626	XM_031901229.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa57627	XM_031901230.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa57628	XM_031901231.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa57629	XM_031901232.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa57630	XM_031901233.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa57631	XM_031901235.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa57632	XM_031901236.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa57633	XM_031901237.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa57634	XM_031901238.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa57635	XM_031901240.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa57636	XM_031901241.1 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa33044	XM_012961765.2 Latest version!	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

magi1 ( XM_012961759.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817213.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa33038
Clone ID Related Accession (Same CDS sequence)	XM_012961759.2
Accession Version	XM_012961759.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961759.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGAATTGCCC 
GCTGGATGGG AGAAAATTGA AGACCCGGTT TATGGAGTCT ACTATGTAGA TCACATAAAC 
AAGAAAACGC AGTATGAAAA TCCTGTATTG GAGGCCAAAC GAAAGAAACA GCTTGGACAG 
CAGCAAAGTG AAGAATGGAC AGGGGACCAT CCAACCATTG CTCCGCCCCC CATAATGCTT 
CCTAACAACC TTACTCCAAA AAAGAAGGAA CCAGCAAGAG AAACGGCATC ACATGTGAAA 
CCGTTCTTCA CCAGGAACCC CTCTGAACTG AAAGGCAAGT TCATCCATAC GAAGCTAAGG 
AAAAGTAGTC GGGGCTTTGG CTTTACCGTT GTCGGAGGGG ACGAACCAGA TGAGTTCTTG 
CAAATAAAAA GTTTGGTCGT CGACGGTCCG GCCGCGTTGG ACGGCAAAAT GGAAACGGGT 
GATGTTATCG TTAGCGTGAA CGATATCTGC GTCCTTGGCT ACACGCACGC TCAAGTTGTG 
AAGATCTTCC AGTCTATACC CATTGGGGAA AGTGTGGACC TTGAACTATG CCGGGGTTAT 
CCTCTTCCGT TTGACCCAGA TGACCCCAAC ACCAGCCTTG TGACATCAGT TGCAATATTG 
GACAAAGACC CTATCATTGT CAATGGGCAA GAGAATTATG ACTCTCCGTC GAGCAACAGC 
AGTAAAACTA GAAAAGCCAA TAACGTCAAG GATCCAAGGC CTAGCAGCCC TGCTGACATT 
TCTTCAAATG GTTCCCATGG TTACCCTAAT GATACAGTGT CCTTGGCATC CTCTATAGCT 
ACGCAACCTG AACTAATTAC TGTGCATATA GTAAAAGGAC CAATGGGATT TGGCTTTACC 
ATAGCAGACA GTCCTGGCGG TGGGGGCCAG AGAGTGAAGC AGATTGTAGA TAGTCCAAGA 
TGCCGGGGCC TCAAAGAAGG AGATCTTATA GTAGAGGTGA ATAAAAAGAA TGTTCAGAAC 
CTTACCCATA ACCAAGTTGT GGATTTACTC ATCGAATGTC CTAAGGGAAG CGAAGTTACC 
CTACTGGTTC AAAGAGGAGG ACTCCCGGTC CCAAAGAAGA GCCCAAAGTC GCAACCACTT 
GAAAGGAAAG ACAGCCAAAA CAGCTCGCAG CACAGTGTTT CCAGCCAGCA CAGTGGGCAC 
ACTGCTTCAC CCCATCACGC CGCGCCTGGG CTCCCCGATT ATCCTACCAG CGAGGCGCCG 
GCTGTCGACC AAGCCGACAA CTCCGGGCAG AAAAAGCCTG ATCCTTTCAA AATCTGGGCC 
CAATCCAGGA GCATGTATGA GAACAGACTT CCAGACTTTC AAGAGCATGA TATATTTTTG 
TGGCGAAAGG AAACTGGATT TGGGTTTCGA ATTCTCGGTG GAAATGAGCC TGGAGAACCA 
ATCTTCATAG GTCATATTGT GCCACTGGGG TCAGCGGATG CCGACGGGCG GTTGCGCTCT 
GGTGACGAGC TTATCTGTGT GGATGGAACA GCGGTTGTTG GAAAATCTCA TCAACTCGTC 
GTACAGCTTA TGCAACAGGC TGCGAAGCAA GGACACGTCA ATCTAACGAT CAGACGAAAA 
ACGATCTATC CGGTTCCAAA GGCAGAGAAC GAAGCTCCTG CATCACCAGC ATCCTCGCAC 
CATAGCAGCA CCCAGCCGGC GTCTCTAACG GAGGAGAAGC GGACGCCCCA AGGCAGCCAA 
AACTCCTTAA ACACTGTCAG TTCAGGCAGC GGCAGCACCA GCGGCATTGG CAGTGGCGGA 
GGCGGCGGGA GCGGCAACGT CAGCACCGTG GTTCAGCCGT ATGATGTCGA GATCCGACGC 
GGTGAAAATG AAGGATTTGG ATTTGTCATT GTGTCTTCTG TCAGCCGGCC AGAAGCCGGG 
ACTACTTTTG CCGGCAATGC ATGTGTGGCC ATGCCTCACA AAATAGGTCG GATAATAGAG 
GGGAGCCCCG CGGATCGCTG CGGCAAGCTG AAAGTGGGGG ACCGGATCTT GGCCGTTAAC 
GGGTGCTCCA TCACCAACAA GTCACACTCG GACATCGTCA ATTTAATAAA GGAAGCGGGG 
AACACCGTGA CTCTGCGCAT CATTCCCGGC GATGAAACGT CCAACGCGTC TTTATTAACC 
AATGCAGAGA AGATTGCCAC GATTACGACC ACGCATACGC CACAAACGAT ACCCCAAGAA 
ACAAAAAATA ATGCAAAACC AAAGCCGGAG CCCCAGTTTG AATTCAAGAA CTCACCAGCC 
ACACAGCCTG ATCAAGAAAT TTATTCAGTG GAACTAGACC GAGGATTAAA AGGATTTGGG 
TTTAGTCTAC GAGGAGGTCG AGAATATAAC ATGGATCTCT ATGTTTTACG CCTTGCGGAG 
GATGGGCCTG CAGCCAGATG TGGGAAAATG AGGGTTGGCG ATGAAATCCT GGAAATAAAC 
GGAGAGACCA CAAAGAACAT GAAGCACGCC CGGGCAATAG AACTGATTAA AAATGGTGGC 
CGAAGAGTAC GCCTGTGTCT GAAACGCGGA GATGGTTCCG TACCGGAATA TGAACCCGGC 
GTTGACGGAA ACAGCCCCAC AACCAGTCCA CAAAGTGTAT CGAAAATGAA CTCGTTACCA 
TCTGATAGAA ATCACTCATC ACTGGAGTTC AATTATCCAT CCGATCTTCA CAAATCATTA 
CAGCATGGAG ACAAACGAGC TTACGTTAAA GACCCTAAAG GCAACAGGGA GTATGGCAGA 
CCACATAACG AACATCACAC TTGGAATGGA TCTTCTAAAA ATTATTCTGT ACAAAATAAC 
AGAAAAGATC GATCACCTGA GGGGAGGCGA CCAAGACAGG CCGATCGAAC AATGACGAAC 
GGTCAGAAAA GACAGTCTCC CGAGAAAAGA AATGAAGGAA CTAGGAGTGC TGAGAACACA 
CTGGAAAGGA GAGAGAAAAA TGAGAGAAGA AAAGAGGTTT CTCCTCAAAA GCCCAGGGAA 
AGGTCGCCGG TGCGCAGAAG GGATGGTTCC CCAACCAGGC GAAGGAAATC CTTGGATAGA 
TTTATGGATC AAAGACGATC CCCTGATAGG AGAAGAGAGA GTTCACCTGA CCGAAGAAGA 
GCAAAGTCAA CGGACAGGAG ACGAGAAAGG TCACCAGAAA GGAGGAGAGA AAGGTCTCCT 
GAGAGAAGAA GAGAGAAGTC CACTGAGAGA AGAAGGGACA GGTCTCCTGA TAAACGAAAC 
AAAGAGCAAC GAACCAGCAA CAGAGACAGG GATGAACACA GTCTGAAATA TGATACCCTC 
AGAAGTGGCA GGCACCAACA AGAACAACGG AGGAGGCAAT ACAAGGAATG CAGCACCGAC 
CTGAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817213.1
CDS	284..4675
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_012961759.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961759.2

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGAATTGCCC 
GCTGGATGGG AGAAAATTGA AGACCCGGTT TATGGAGTCT ACTATGTAGA TCACATAAAC 
AAGAAAACGC AGTATGAAAA TCCTGTATTG GAGGCCAAAC GAAAGAAACA GCTTGGACAG 
CAGCAAAGTG AAGAATGGAC AGGGGACCAT CCAACCATTG CTCCGCCCCC CATAATGCTT 
CCTAACAACC TTACTCCAAA AAAGAAGGAA CCAGCAAGAG AAACGGCATC ACATGTGAAA 
CCGTTCTTCA CCAGGAACCC CTCTGAACTG AAAGGCAAGT TCATCCATAC GAAGCTAAGG 
AAAAGTAGTC GGGGCTTTGG CTTTACCGTT GTCGGAGGGG ACGAACCAGA TGAGTTCTTG 
CAAATAAAAA GTTTGGTCGT CGACGGTCCG GCCGCGTTGG ACGGCAAAAT GGAAACGGGT 
GATGTTATCG TTAGCGTGAA CGATATCTGC GTCCTTGGCT ACACGCACGC TCAAGTTGTG 
AAGATCTTCC AGTCTATACC CATTGGGGAA AGTGTGGACC TTGAACTATG CCGGGGTTAT 
CCTCTTCCGT TTGACCCAGA TGACCCCAAC ACCAGCCTTG TGACATCAGT TGCAATATTG 
GACAAAGACC CTATCATTGT CAATGGGCAA GAGAATTATG ACTCTCCGTC GAGCAACAGC 
AGTAAAACTA GAAAAGCCAA TAACGTCAAG GATCCAAGGC CTAGCAGCCC TGCTGACATT 
TCTTCAAATG GTTCCCATGG TTACCCTAAT GATACAGTGT CCTTGGCATC CTCTATAGCT 
ACGCAACCTG AACTAATTAC TGTGCATATA GTAAAAGGAC CAATGGGATT TGGCTTTACC 
ATAGCAGACA GTCCTGGCGG TGGGGGCCAG AGAGTGAAGC AGATTGTAGA TAGTCCAAGA 
TGCCGGGGCC TCAAAGAAGG AGATCTTATA GTAGAGGTGA ATAAAAAGAA TGTTCAGAAC 
CTTACCCATA ACCAAGTTGT GGATTTACTC ATCGAATGTC CTAAGGGAAG CGAAGTTACC 
CTACTGGTTC AAAGAGGAGG ACTCCCGGTC CCAAAGAAGA GCCCAAAGTC GCAACCACTT 
GAAAGGAAAG ACAGCCAAAA CAGCTCGCAG CACAGTGTTT CCAGCCAGCA CAGTGGGCAC 
ACTGCTTCAC CCCATCACGC CGCGCCTGGG CTCCCCGATT ATCCTACCAG CGAGGCGCCG 
GCTGTCGACC AAGCCGACAA CTCCGGGCAG AAAAAGCCTG ATCCTTTCAA AATCTGGGCC 
CAATCCAGGA GCATGTATGA GAACAGACTT CCAGACTTTC AAGAGCATGA TATATTTTTG 
TGGCGAAAGG AAACTGGATT TGGGTTTCGA ATTCTCGGTG GAAATGAGCC TGGAGAACCA 
ATCTTCATAG GTCATATTGT GCCACTGGGG TCAGCGGATG CCGACGGGCG GTTGCGCTCT 
GGTGACGAGC TTATCTGTGT GGATGGAACA GCGGTTGTTG GAAAATCTCA TCAACTCGTC 
GTACAGCTTA TGCAACAGGC TGCGAAGCAA GGACACGTCA ATCTAACGAT CAGACGAAAA 
ACGATCTATC CGGTTCCAAA GGCAGAGAAC GAAGCTCCTG CATCACCAGC ATCCTCGCAC 
CATAGCAGCA CCCAGCCGGC GTCTCTAACG GAGGAGAAGC GGACGCCCCA AGGCAGCCAA 
AACTCCTTAA ACACTGTCAG TTCAGGCAGC GGCAGCACCA GCGGCATTGG CAGTGGCGGA 
GGCGGCGGGA GCGGCAACGT CAGCACCGTG GTTCAGCCGT ATGATGTCGA GATCCGACGC 
GGTGAAAATG AAGGATTTGG ATTTGTCATT GTGTCTTCTG TCAGCCGGCC AGAAGCCGGG 
ACTACTTTTG CCGGCAATGC ATGTGTGGCC ATGCCTCACA AAATAGGTCG GATAATAGAG 
GGGAGCCCCG CGGATCGCTG CGGCAAGCTG AAAGTGGGGG ACCGGATCTT GGCCGTTAAC 
GGGTGCTCCA TCACCAACAA GTCACACTCG GACATCGTCA ATTTAATAAA GGAAGCGGGG 
AACACCGTGA CTCTGCGCAT CATTCCCGGC GATGAAACGT CCAACGCGTC TTTATTAACC 
AATGCAGAGA AGATTGCCAC GATTACGACC ACGCATACGC CACAAACGAT ACCCCAAGAA 
ACAAAAAATA ATGCAAAACC AAAGCCGGAG CCCCAGTTTG AATTCAAGAA CTCACCAGCC 
ACACAGCCTG ATCAAGAAAT TTATTCAGTG GAACTAGACC GAGGATTAAA AGGATTTGGG 
TTTAGTCTAC GAGGAGGTCG AGAATATAAC ATGGATCTCT ATGTTTTACG CCTTGCGGAG 
GATGGGCCTG CAGCCAGATG TGGGAAAATG AGGGTTGGCG ATGAAATCCT GGAAATAAAC 
GGAGAGACCA CAAAGAACAT GAAGCACGCC CGGGCAATAG AACTGATTAA AAATGGTGGC 
CGAAGAGTAC GCCTGTGTCT GAAACGCGGA GATGGTTCCG TACCGGAATA TGAACCCGGC 
GTTGACGGAA ACAGCCCCAC AACCAGTCCA CAAAGTGTAT CGAAAATGAA CTCGTTACCA 
TCTGATAGAA ATCACTCATC ACTGGAGTTC AATTATCCAT CCGATCTTCA CAAATCATTA 
CAGCATGGAG ACAAACGAGC TTACGTTAAA GACCCTAAAG GCAACAGGGA GTATGGCAGA 
CCACATAACG AACATCACAC TTGGAATGGA TCTTCTAAAA ATTATTCTGT ACAAAATAAC 
AGAAAAGATC GATCACCTGA GGGGAGGCGA CCAAGACAGG CCGATCGAAC AATGACGAAC 
GGTCAGAAAA GACAGTCTCC CGAGAAAAGA AATGAAGGAA CTAGGAGTGC TGAGAACACA 
CTGGAAAGGA GAGAGAAAAA TGAGAGAAGA AAAGAGGTTT CTCCTCAAAA GCCCAGGGAA 
AGGTCGCCGG TGCGCAGAAG GGATGGTTCC CCAACCAGGC GAAGGAAATC CTTGGATAGA 
TTTATGGATC AAAGACGATC CCCTGATAGG AGAAGAGAGA GTTCACCTGA CCGAAGAAGA 
GCAAAGTCAA CGGACAGGAG ACGAGAAAGG TCACCAGAAA GGAGGAGAGA AAGGTCTCCT 
GAGAGAAGAA GAGAGAAGTC CACTGAGAGA AGAAGGGACA GGTCTCCTGA TAAACGAAAC 
AAAGAGCAAC GAACCAGCAA CAGAGACAGG GATGAACACA GTCTGAAATA TGATACCCTC 
AGAAGTGGCA GGCACCAACA AGAACAACGG AGGAGGCAAT ACAAGGAATG CAGCACCGAC 
CTGAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_012961760.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817214.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa33039
Clone ID Related Accession (Same CDS sequence)	XM_012961760.2
Accession Version	XM_012961760.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961760.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGGGGT ACACACCGAG GAACTGGACA GTGAACTAGA ATTGCCCGCT 
GGATGGGAGA AAATTGAAGA CCCGGTTTAT GGAGTCTACT ATGTAGATCA CATAAACAAG 
AAAACGCAGT ATGAAAATCC TGTATTGGAG GCCAAACGAA AGAAACAGCT TGGACAGCAG 
CAAAGTGAAG AATGGACAGG GGACCATCCA ACCATTGCTC CGCCCCCCAT AATGCTTCCT 
AACAACCTTA CTCCAAAAAA GAAGGAACCA GCAAGAGAAA CGGCATCACA TGTGAAACCG 
TTCTTCACCA GGAACCCCTC TGAACTGAAA GGCAAGTTCA TCCATACGAA GCTAAGGAAA 
AGTAGTCGGG GCTTTGGCTT TACCGTTGTC GGAGGGGACG AACCAGATGA GTTCTTGCAA 
ATAAAAAGTT TGGTCGTCGA CGGTCCGGCC GCGTTGGACG GCAAAATGGA AACGGGTGAT 
GTTATCGTTA GCGTGAACGA TATCTGCGTC CTTGGCTACA CGCACGCTCA AGTTGTGAAG 
ATCTTCCAGT CTATACCCAT TGGGGAAAGT GTGGACCTTG AACTATGCCG GGGTTATCCT 
CTTCCGTTTG ACCCAGATGA CCCCAACACC AGCCTTGTGA CATCAGTTGC AATATTGGAC 
AAAGACCCTA TCATTGTCAA TGGGCAAGAG AATTATGACT CTCCGTCGAG CAACAGCAGT 
AAAACTAGAA AAGCCAATAA CGTCAAGGAT CCAAGGCCTA GCAGCCCTGC TGACATTTCT 
TCAAATGGTT CCCATGGTTA CCCTAATGAT ACAGTGTCCT TGGCATCCTC TATAGCTACG 
CAACCTGAAC TAATTACTGT GCATATAGTA AAAGGACCAA TGGGATTTGG CTTTACCATA 
GCAGACAGTC CTGGCGGTGG GGGCCAGAGA GTGAAGCAGA TTGTAGATAG TCCAAGATGC 
CGGGGCCTCA AAGAAGGAGA TCTTATAGTA GAGGTGAATA AAAAGAATGT TCAGAACCTT 
ACCCATAACC AAGTTGTGGA TTTACTCATC GAATGTCCTA AGGGAAGCGA AGTTACCCTA 
CTGGTTCAAA GAGGAGGACT CCCGGTCCCA AAGAAGAGCC CAAAGTCGCA ACCACTTGAA 
AGGAAAGACA GCCAAAACAG CTCGCAGCAC AGTGTTTCCA GCCAGCACAG TGGGCACACT 
GCTTCACCCC ATCACGCCGC GCCTGGGCTC CCCGATTATC CTACCAGCGA GGCGCCGGCT 
GTCGACCAAG CCGACAACTC CGGGCAGAAA AAGCCTGATC CTTTCAAAAT CTGGGCCCAA 
TCCAGGAGCA TGTATGAGAA CAGACTTCCA GACTTTCAAG AGCATGATAT ATTTTTGTGG 
CGAAAGGAAA CTGGATTTGG GTTTCGAATT CTCGGTGGAA ATGAGCCTGG AGAACCAATC 
TTCATAGGTC ATATTGTGCC ACTGGGGTCA GCGGATGCCG ACGGGCGGTT GCGCTCTGGT 
GACGAGCTTA TCTGTGTGGA TGGAACAGCG GTTGTTGGAA AATCTCATCA ACTCGTCGTA 
CAGCTTATGC AACAGGCTGC GAAGCAAGGA CACGTCAATC TAACGATCAG ACGAAAAACG 
ATCTATCCGG TTCCAAAGGC AGAGAACGAA GCTCCTGCAT CACCAGCATC CTCGCACCAT 
AGCAGCACCC AGCCGGCGTC TCTAACGGAG GAGAAGCGGA CGCCCCAAGG CAGCCAAAAC 
TCCTTAAACA CTGTCAGTTC AGGCAGCGGC AGCACCAGCG GCATTGGCAG TGGCGGAGGC 
GGCGGGAGCG GCAACGTCAG CACCGTGGTT CAGCCGTATG ATGTCGAGAT CCGACGCGGT 
GAAAATGAAG GATTTGGATT TGTCATTGTG TCTTCTGTCA GCCGGCCAGA AGCCGGGACT 
ACTTTTGCCG GCAATGCATG TGTGGCCATG CCTCACAAAA TAGGTCGGAT AATAGAGGGG 
AGCCCCGCGG ATCGCTGCGG CAAGCTGAAA GTGGGGGACC GGATCTTGGC CGTTAACGGG 
TGCTCCATCA CCAACAAGTC ACACTCGGAC ATCGTCAATT TAATAAAGGA AGCGGGGAAC 
ACCGTGACTC TGCGCATCAT TCCCGGCGAT GAAACGTCCA ACGCGTCTTT ATTAACCAAT 
GCAGAGAAGA TTGCCACGAT TACGACCACG CATACGCCAC AAACGATACC CCAAGAAACA 
AAAAATAATG CAAAACCAAA GCCGGAGCCC CAGTTTGAAT TCAAGAACTC ACCAGCCACA 
CAGCCTGATC AAGAAATTTA TTCAGTGGAA CTAGACCGAG GATTAAAAGG ATTTGGGTTT 
AGTCTACGAG GAGGTCGAGA ATATAACATG GATCTCTATG TTTTACGCCT TGCGGAGGAT 
GGGCCTGCAG CCAGATGTGG GAAAATGAGG GTTGGCGATG AAATCCTGGA AATAAACGGA 
GAGACCACAA AGAACATGAA GCACGCCCGG GCAATAGAAC TGATTAAAAA TGGTGGCCGA 
AGAGTACGCC TGTGTCTGAA ACGCGGAGAT GGTTCCGTAC CGGAATATGA ACCCGGCGTT 
GACGGAAACA GCCCCACAAC CAGTCCACAA AGTGTATCGA AAATGAACTC GTTACCATCT 
GATAGAAATC ACTCATCACT GGAGTTCAAT TATCCATCCG ATCTTCACAA ATCATTACAG 
CATGGAGACA AACGAGCTTA CGTTAAAGAC CCTAAAGGCA ACAGGGAGTA TGGCAGACCA 
CATAACGAAC ATCACACTTG GAATGGATCT TCTAAAAATT ATTCTGTACA AAATAACAGA 
AAAGATCGAT CACCTGAGGG GAGGCGACCA AGACAGGCCG ATCGAACAAT GACGAACGGT 
CAGAAAAGAC AGTCTCCCGA GAAAAGAAAT GAAGGAACTA GGAGTGCTGA GAACACACTG 
GAAAGGAGAG AGAAAAATGA GAGAAGAAAA GAGGTTTCTC CTCAAAAGCC CAGGGAAAGG 
TCGCCGGTGC GCAGAAGGGA TGGTTCCCCA ACCAGGCGAA GGAAATCCTT GGATAGATTT 
ATGGATCAAA GACGATCCCC TGATAGGAGA AGAGAGAGTT CACCTGACCG AAGAAGAGCA 
AAGTCAACGG ACAGGAGACG AGAAAGGTCA CCAGAAAGGA GGAGAGAAAG GTCTCCTGAG 
AGAAGAAGAG AGAAGTCCAC TGAGAGAAGA AGGGACAGGT CTCCTGATAA ACGAAACAAA 
GAGCAACGAA CCAGCAACAG AGACAGGGAT GAACACAGTC TGAAATATGA TACCCTCAGA 
AGTGGCAGGC ACCAACAAGA ACAACGGAGG AGGCAATACA AGGAATGCAG CACCGACCTG 
AGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817214.1
CDS	284..4672
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_012961760.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961760.2

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGGGGT ACACACCGAG GAACTGGACA GTGAACTAGA ATTGCCCGCT 
GGATGGGAGA AAATTGAAGA CCCGGTTTAT GGAGTCTACT ATGTAGATCA CATAAACAAG 
AAAACGCAGT ATGAAAATCC TGTATTGGAG GCCAAACGAA AGAAACAGCT TGGACAGCAG 
CAAAGTGAAG AATGGACAGG GGACCATCCA ACCATTGCTC CGCCCCCCAT AATGCTTCCT 
AACAACCTTA CTCCAAAAAA GAAGGAACCA GCAAGAGAAA CGGCATCACA TGTGAAACCG 
TTCTTCACCA GGAACCCCTC TGAACTGAAA GGCAAGTTCA TCCATACGAA GCTAAGGAAA 
AGTAGTCGGG GCTTTGGCTT TACCGTTGTC GGAGGGGACG AACCAGATGA GTTCTTGCAA 
ATAAAAAGTT TGGTCGTCGA CGGTCCGGCC GCGTTGGACG GCAAAATGGA AACGGGTGAT 
GTTATCGTTA GCGTGAACGA TATCTGCGTC CTTGGCTACA CGCACGCTCA AGTTGTGAAG 
ATCTTCCAGT CTATACCCAT TGGGGAAAGT GTGGACCTTG AACTATGCCG GGGTTATCCT 
CTTCCGTTTG ACCCAGATGA CCCCAACACC AGCCTTGTGA CATCAGTTGC AATATTGGAC 
AAAGACCCTA TCATTGTCAA TGGGCAAGAG AATTATGACT CTCCGTCGAG CAACAGCAGT 
AAAACTAGAA AAGCCAATAA CGTCAAGGAT CCAAGGCCTA GCAGCCCTGC TGACATTTCT 
TCAAATGGTT CCCATGGTTA CCCTAATGAT ACAGTGTCCT TGGCATCCTC TATAGCTACG 
CAACCTGAAC TAATTACTGT GCATATAGTA AAAGGACCAA TGGGATTTGG CTTTACCATA 
GCAGACAGTC CTGGCGGTGG GGGCCAGAGA GTGAAGCAGA TTGTAGATAG TCCAAGATGC 
CGGGGCCTCA AAGAAGGAGA TCTTATAGTA GAGGTGAATA AAAAGAATGT TCAGAACCTT 
ACCCATAACC AAGTTGTGGA TTTACTCATC GAATGTCCTA AGGGAAGCGA AGTTACCCTA 
CTGGTTCAAA GAGGAGGACT CCCGGTCCCA AAGAAGAGCC CAAAGTCGCA ACCACTTGAA 
AGGAAAGACA GCCAAAACAG CTCGCAGCAC AGTGTTTCCA GCCAGCACAG TGGGCACACT 
GCTTCACCCC ATCACGCCGC GCCTGGGCTC CCCGATTATC CTACCAGCGA GGCGCCGGCT 
GTCGACCAAG CCGACAACTC CGGGCAGAAA AAGCCTGATC CTTTCAAAAT CTGGGCCCAA 
TCCAGGAGCA TGTATGAGAA CAGACTTCCA GACTTTCAAG AGCATGATAT ATTTTTGTGG 
CGAAAGGAAA CTGGATTTGG GTTTCGAATT CTCGGTGGAA ATGAGCCTGG AGAACCAATC 
TTCATAGGTC ATATTGTGCC ACTGGGGTCA GCGGATGCCG ACGGGCGGTT GCGCTCTGGT 
GACGAGCTTA TCTGTGTGGA TGGAACAGCG GTTGTTGGAA AATCTCATCA ACTCGTCGTA 
CAGCTTATGC AACAGGCTGC GAAGCAAGGA CACGTCAATC TAACGATCAG ACGAAAAACG 
ATCTATCCGG TTCCAAAGGC AGAGAACGAA GCTCCTGCAT CACCAGCATC CTCGCACCAT 
AGCAGCACCC AGCCGGCGTC TCTAACGGAG GAGAAGCGGA CGCCCCAAGG CAGCCAAAAC 
TCCTTAAACA CTGTCAGTTC AGGCAGCGGC AGCACCAGCG GCATTGGCAG TGGCGGAGGC 
GGCGGGAGCG GCAACGTCAG CACCGTGGTT CAGCCGTATG ATGTCGAGAT CCGACGCGGT 
GAAAATGAAG GATTTGGATT TGTCATTGTG TCTTCTGTCA GCCGGCCAGA AGCCGGGACT 
ACTTTTGCCG GCAATGCATG TGTGGCCATG CCTCACAAAA TAGGTCGGAT AATAGAGGGG 
AGCCCCGCGG ATCGCTGCGG CAAGCTGAAA GTGGGGGACC GGATCTTGGC CGTTAACGGG 
TGCTCCATCA CCAACAAGTC ACACTCGGAC ATCGTCAATT TAATAAAGGA AGCGGGGAAC 
ACCGTGACTC TGCGCATCAT TCCCGGCGAT GAAACGTCCA ACGCGTCTTT ATTAACCAAT 
GCAGAGAAGA TTGCCACGAT TACGACCACG CATACGCCAC AAACGATACC CCAAGAAACA 
AAAAATAATG CAAAACCAAA GCCGGAGCCC CAGTTTGAAT TCAAGAACTC ACCAGCCACA 
CAGCCTGATC AAGAAATTTA TTCAGTGGAA CTAGACCGAG GATTAAAAGG ATTTGGGTTT 
AGTCTACGAG GAGGTCGAGA ATATAACATG GATCTCTATG TTTTACGCCT TGCGGAGGAT 
GGGCCTGCAG CCAGATGTGG GAAAATGAGG GTTGGCGATG AAATCCTGGA AATAAACGGA 
GAGACCACAA AGAACATGAA GCACGCCCGG GCAATAGAAC TGATTAAAAA TGGTGGCCGA 
AGAGTACGCC TGTGTCTGAA ACGCGGAGAT GGTTCCGTAC CGGAATATGA ACCCGGCGTT 
GACGGAAACA GCCCCACAAC CAGTCCACAA AGTGTATCGA AAATGAACTC GTTACCATCT 
GATAGAAATC ACTCATCACT GGAGTTCAAT TATCCATCCG ATCTTCACAA ATCATTACAG 
CATGGAGACA AACGAGCTTA CGTTAAAGAC CCTAAAGGCA ACAGGGAGTA TGGCAGACCA 
CATAACGAAC ATCACACTTG GAATGGATCT TCTAAAAATT ATTCTGTACA AAATAACAGA 
AAAGATCGAT CACCTGAGGG GAGGCGACCA AGACAGGCCG ATCGAACAAT GACGAACGGT 
CAGAAAAGAC AGTCTCCCGA GAAAAGAAAT GAAGGAACTA GGAGTGCTGA GAACACACTG 
GAAAGGAGAG AGAAAAATGA GAGAAGAAAA GAGGTTTCTC CTCAAAAGCC CAGGGAAAGG 
TCGCCGGTGC GCAGAAGGGA TGGTTCCCCA ACCAGGCGAA GGAAATCCTT GGATAGATTT 
ATGGATCAAA GACGATCCCC TGATAGGAGA AGAGAGAGTT CACCTGACCG AAGAAGAGCA 
AAGTCAACGG ACAGGAGACG AGAAAGGTCA CCAGAAAGGA GGAGAGAAAG GTCTCCTGAG 
AGAAGAAGAG AGAAGTCCAC TGAGAGAAGA AGGGACAGGT CTCCTGATAA ACGAAACAAA 
GAGCAACGAA CCAGCAACAG AGACAGGGAT GAACACAGTC TGAAATATGA TACCCTCAGA 
AGTGGCAGGC ACCAACAAGA ACAACGGAGG AGGCAATACA AGGAATGCAG CACCGACCTG 
AGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_012961761.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817215.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa33040
Clone ID Related Accession (Same CDS sequence)	XM_012961761.2
Accession Version	XM_012961761.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961761.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGAATTGCCC 
GCTGGATGGG AGAAAATTGA AGACCCGGTT TATGGAGTCT ACTATGTAGA TCACATAAAC 
AAGAAAACGC AGTATGAAAA TCCTGTATTG GAGGCCAAAC GAAAGAAACA GCTTGGACAG 
CAGCAAAGTG AAGAATGGAC AGGGGACCAT CCAACCATTG CTCCGCCCCC CATAATGCTT 
CCTAACAACC TTACTCCAAA AAAGAAGGAA CCAGCAAGAG AAACGGCATC ACATGTGAAA 
CCGTTCTTCA CCAGGAACCC CTCTGAACTG AAAGGCAAGT TCATCCATAC GAAGCTAAGG 
AAAAGTAGTC GGGGCTTTGG CTTTACCGTT GTCGGAGGGG ACGAACCAGA TGAGTTCTTG 
CAAATAAAAA GTTTGGTCGT CGACGGTCCG GCCGCGTTGG ACGGCAAAAT GGAAACGGGT 
GATGTTATCG TTAGCGTGAA CGATATCTGC GTCCTTGGCT ACACGCACGC TCAAGTTGTG 
AAGATCTTCC AGTCTATACC CATTGGGGAA AGTGTGGACC TTGAACTATG CCGGGGTTAT 
CCTCTTCCGT TTGACCCAGA TGACCCCAAC ACCAGCCTTG TGACATCAGT TGCAATATTG 
GACAAAGACC CTATCATTGT CAATGGGCAA GAGAATTATG ACTCTCCGTC GAGCAACAGC 
AGTAAAACTA GAAAAGCCAA TAACGTCAAG GATCCAAGGC CTAGCAGCCC TGCTGACATT 
TCTTCAAATG GTTCCCATGG TTACCCTAAT GATACAGTGT CCTTGGCATC CTCTATAGCT 
ACGCAACCTG AACTAATTAC TGTGCATATA GTAAAAGGAC CAATGGGATT TGGCTTTACC 
ATAGCAGACA GTCCTGGCGG TGGGGGCCAG AGAGTGAAGC AGATTGTAGA TAGTCCAAGA 
TGCCGGGGCC TCAAAGAAGG AGATCTTATA GTAGAGGTGA ATAAAAAGAA TGTTCAGAAC 
CTTACCCATA ACCAAGTTGT GGATTTACTC ATCGAATGTC CTAAGGGAAG CGAAGTTACC 
CTACTGGTTC AAAGAGGAGG ACTCCCGGTC CCAAAGAAGA GCCCAAAGTC GCAACCACTT 
GAAAGGAAAG ACAGCCAAAA CAGCTCGCAG CACAGTGTTT CCAGCCAGCA CAGTGGGCAC 
ACTGCTTCAC CCCATCACGC CGCGCCTGGG CTCCCCGATT ATCCTACCAG CGAGGCGCCG 
GCTGTCGACC AAGCCGACAA CTCCGGGCAG AAAAAGCCTG ATCCTTTCAA AATCTGGGCC 
CAATCCAGGA GCATGTATGA GAACAGACTT CCAGACTTTC AAGAGCATGA TATATTTTTG 
TGGCGAAAGG AAACTGGATT TGGGTTTCGA ATTCTCGGTG GAAATGAGCC TGGAGAACCA 
ATCTTCATAG GTCATATTGT GCCACTGGGG TCAGCGGATG CCGACGGGCG GTTGCGCTCT 
GGTGACGAGC TTATCTGTGT GGATGGAACA GCGGTTGTTG GAAAATCTCA TCAACTCGTC 
GTACAGCTTA TGCAACAGGC TGCGAAGCAA GGACACGTCA ATCTAACGAT CAGACGAAAA 
ACGATCTATC CGGTTCCAAA GGCAGAGAAC GAAGCTCCTG CATCACCAGC ATCCTCGCAC 
CATAGCAGCA CCCAGCCGGC GTCTCTAACG GAGGAGAAGC GGACGCCCCA AGGCAGCCAA 
AACTCCTTAA ACACTGTCAG TTCAGGCAGC GGCAGCACCA GCGGCATTGG CAGTGGCGGA 
GGCGGCGGGA GCGGCAACGT CAGCACCGTG GTTCAGCCGT ATGATGTCGA GATCCGACGC 
GGTGAAAATG AAGGATTTGG ATTTGTCATT GTGTCTTCTG TCAGCCGGCC AGAAGCCGGG 
ACTACTTTTG GTCGGATAAT AGAGGGGAGC CCCGCGGATC GCTGCGGCAA GCTGAAAGTG 
GGGGACCGGA TCTTGGCCGT TAACGGGTGC TCCATCACCA ACAAGTCACA CTCGGACATC 
GTCAATTTAA TAAAGGAAGC GGGGAACACC GTGACTCTGC GCATCATTCC CGGCGATGAA 
ACGTCCAACG CGTCTTTATT AACCAATGCA GAGAAGATTG CCACGATTAC GACCACGCAT 
ACGCCACAAA CGATACCCCA AGAAACAAAA AATAATGCAA AACCAAAGCC GGAGCCCCAG 
TTTGAATTCA AGAACTCACC AGCCACACAG CCTGATCAAG AAATTTATTC AGTGGAACTA 
GACCGAGGAT TAAAAGGATT TGGGTTTAGT CTACGAGGAG GTCGAGAATA TAACATGGAT 
CTCTATGTTT TACGCCTTGC GGAGGATGGG CCTGCAGCCA GATGTGGGAA AATGAGGGTT 
GGCGATGAAA TCCTGGAAAT AAACGGAGAG ACCACAAAGA ACATGAAGCA CGCCCGGGCA 
ATAGAACTGA TTAAAAATGG TGGCCGAAGA GTACGCCTGT GTCTGAAACG CGGAGATGGT 
TCCGTACCGG AATATGAACC CGGCGTTGAC GGAAACAGCC CCACAACCAG TCCACAAAGT 
GTATCGAAAA TGAACTCGTT ACCATCTGAT AGAAATCACT CATCACTGGA GTTCAATTAT 
CCATCCGATC TTCACAAATC ATTACAGCAT GGAGACAAAC GAGCTTACGT TAAAGACCCT 
AAAGGCAACA GGGAGTATGG CAGACCACAT AACGAACATC ACACTTGGAA TGGATCTTCT 
AAAAATTATT CTGTACAAAA TAACAGAAAA GATCGATCAC CTGAGGGGAG GCGACCAAGA 
CAGGCCGATC GAACAATGAC GAACGGTCAG AAAAGACAGT CTCCCGAGAA AAGAAATGAA 
GGAACTAGGA GTGCTGAGAA CACACTGGAA AGGAGAGAGA AAAATGAGAG AAGAAAAGAG 
GTTTCTCCTC AAAAGCCCAG GGAAAGGTCG CCGGTGCGCA GAAGGGATGG TTCCCCAACC 
AGGCGAAGGA AATCCTTGGA TAGATTTATG GATCAAAGAC GATCCCCTGA TAGGAGAAGA 
GAGAGTTCAC CTGACCGAAG AAGAGCAAAG TCAACGGACA GGAGACGAGA AAGGTCACCA 
GAAAGGAGGA GAGAAAGGTC TCCTGAGAGA AGAAGAGAGA AGTCCACTGA GAGAAGAAGG 
GACAGGTCTC CTGATAAACG AAACAAAGAG CAACGAACCA GCAACAGAGA CAGGGATGAA 
CACAGTCTGA AATATGATAC CCTCAGAAGT GGCAGGCACC AACAAGAACA ACGGAGGAGG 
CAATACAAGG AATGCAGCAC CGACCTGAGC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817215.1
CDS	284..4639
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_012961761.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961761.2

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGAATTGCCC 
GCTGGATGGG AGAAAATTGA AGACCCGGTT TATGGAGTCT ACTATGTAGA TCACATAAAC 
AAGAAAACGC AGTATGAAAA TCCTGTATTG GAGGCCAAAC GAAAGAAACA GCTTGGACAG 
CAGCAAAGTG AAGAATGGAC AGGGGACCAT CCAACCATTG CTCCGCCCCC CATAATGCTT 
CCTAACAACC TTACTCCAAA AAAGAAGGAA CCAGCAAGAG AAACGGCATC ACATGTGAAA 
CCGTTCTTCA CCAGGAACCC CTCTGAACTG AAAGGCAAGT TCATCCATAC GAAGCTAAGG 
AAAAGTAGTC GGGGCTTTGG CTTTACCGTT GTCGGAGGGG ACGAACCAGA TGAGTTCTTG 
CAAATAAAAA GTTTGGTCGT CGACGGTCCG GCCGCGTTGG ACGGCAAAAT GGAAACGGGT 
GATGTTATCG TTAGCGTGAA CGATATCTGC GTCCTTGGCT ACACGCACGC TCAAGTTGTG 
AAGATCTTCC AGTCTATACC CATTGGGGAA AGTGTGGACC TTGAACTATG CCGGGGTTAT 
CCTCTTCCGT TTGACCCAGA TGACCCCAAC ACCAGCCTTG TGACATCAGT TGCAATATTG 
GACAAAGACC CTATCATTGT CAATGGGCAA GAGAATTATG ACTCTCCGTC GAGCAACAGC 
AGTAAAACTA GAAAAGCCAA TAACGTCAAG GATCCAAGGC CTAGCAGCCC TGCTGACATT 
TCTTCAAATG GTTCCCATGG TTACCCTAAT GATACAGTGT CCTTGGCATC CTCTATAGCT 
ACGCAACCTG AACTAATTAC TGTGCATATA GTAAAAGGAC CAATGGGATT TGGCTTTACC 
ATAGCAGACA GTCCTGGCGG TGGGGGCCAG AGAGTGAAGC AGATTGTAGA TAGTCCAAGA 
TGCCGGGGCC TCAAAGAAGG AGATCTTATA GTAGAGGTGA ATAAAAAGAA TGTTCAGAAC 
CTTACCCATA ACCAAGTTGT GGATTTACTC ATCGAATGTC CTAAGGGAAG CGAAGTTACC 
CTACTGGTTC AAAGAGGAGG ACTCCCGGTC CCAAAGAAGA GCCCAAAGTC GCAACCACTT 
GAAAGGAAAG ACAGCCAAAA CAGCTCGCAG CACAGTGTTT CCAGCCAGCA CAGTGGGCAC 
ACTGCTTCAC CCCATCACGC CGCGCCTGGG CTCCCCGATT ATCCTACCAG CGAGGCGCCG 
GCTGTCGACC AAGCCGACAA CTCCGGGCAG AAAAAGCCTG ATCCTTTCAA AATCTGGGCC 
CAATCCAGGA GCATGTATGA GAACAGACTT CCAGACTTTC AAGAGCATGA TATATTTTTG 
TGGCGAAAGG AAACTGGATT TGGGTTTCGA ATTCTCGGTG GAAATGAGCC TGGAGAACCA 
ATCTTCATAG GTCATATTGT GCCACTGGGG TCAGCGGATG CCGACGGGCG GTTGCGCTCT 
GGTGACGAGC TTATCTGTGT GGATGGAACA GCGGTTGTTG GAAAATCTCA TCAACTCGTC 
GTACAGCTTA TGCAACAGGC TGCGAAGCAA GGACACGTCA ATCTAACGAT CAGACGAAAA 
ACGATCTATC CGGTTCCAAA GGCAGAGAAC GAAGCTCCTG CATCACCAGC ATCCTCGCAC 
CATAGCAGCA CCCAGCCGGC GTCTCTAACG GAGGAGAAGC GGACGCCCCA AGGCAGCCAA 
AACTCCTTAA ACACTGTCAG TTCAGGCAGC GGCAGCACCA GCGGCATTGG CAGTGGCGGA 
GGCGGCGGGA GCGGCAACGT CAGCACCGTG GTTCAGCCGT ATGATGTCGA GATCCGACGC 
GGTGAAAATG AAGGATTTGG ATTTGTCATT GTGTCTTCTG TCAGCCGGCC AGAAGCCGGG 
ACTACTTTTG GTCGGATAAT AGAGGGGAGC CCCGCGGATC GCTGCGGCAA GCTGAAAGTG 
GGGGACCGGA TCTTGGCCGT TAACGGGTGC TCCATCACCA ACAAGTCACA CTCGGACATC 
GTCAATTTAA TAAAGGAAGC GGGGAACACC GTGACTCTGC GCATCATTCC CGGCGATGAA 
ACGTCCAACG CGTCTTTATT AACCAATGCA GAGAAGATTG CCACGATTAC GACCACGCAT 
ACGCCACAAA CGATACCCCA AGAAACAAAA AATAATGCAA AACCAAAGCC GGAGCCCCAG 
TTTGAATTCA AGAACTCACC AGCCACACAG CCTGATCAAG AAATTTATTC AGTGGAACTA 
GACCGAGGAT TAAAAGGATT TGGGTTTAGT CTACGAGGAG GTCGAGAATA TAACATGGAT 
CTCTATGTTT TACGCCTTGC GGAGGATGGG CCTGCAGCCA GATGTGGGAA AATGAGGGTT 
GGCGATGAAA TCCTGGAAAT AAACGGAGAG ACCACAAAGA ACATGAAGCA CGCCCGGGCA 
ATAGAACTGA TTAAAAATGG TGGCCGAAGA GTACGCCTGT GTCTGAAACG CGGAGATGGT 
TCCGTACCGG AATATGAACC CGGCGTTGAC GGAAACAGCC CCACAACCAG TCCACAAAGT 
GTATCGAAAA TGAACTCGTT ACCATCTGAT AGAAATCACT CATCACTGGA GTTCAATTAT 
CCATCCGATC TTCACAAATC ATTACAGCAT GGAGACAAAC GAGCTTACGT TAAAGACCCT 
AAAGGCAACA GGGAGTATGG CAGACCACAT AACGAACATC ACACTTGGAA TGGATCTTCT 
AAAAATTATT CTGTACAAAA TAACAGAAAA GATCGATCAC CTGAGGGGAG GCGACCAAGA 
CAGGCCGATC GAACAATGAC GAACGGTCAG AAAAGACAGT CTCCCGAGAA AAGAAATGAA 
GGAACTAGGA GTGCTGAGAA CACACTGGAA AGGAGAGAGA AAAATGAGAG AAGAAAAGAG 
GTTTCTCCTC AAAAGCCCAG GGAAAGGTCG CCGGTGCGCA GAAGGGATGG TTCCCCAACC 
AGGCGAAGGA AATCCTTGGA TAGATTTATG GATCAAAGAC GATCCCCTGA TAGGAGAAGA 
GAGAGTTCAC CTGACCGAAG AAGAGCAAAG TCAACGGACA GGAGACGAGA AAGGTCACCA 
GAAAGGAGGA GAGAAAGGTC TCCTGAGAGA AGAAGAGAGA AGTCCACTGA GAGAAGAAGG 
GACAGGTCTC CTGATAAACG AAACAAAGAG CAACGAACCA GCAACAGAGA CAGGGATGAA 
CACAGTCTGA AATATGATAC CCTCAGAAGT GGCAGGCACC AACAAGAACA ACGGAGGAGG 
CAATACAAGG AATGCAGCAC CGACCTGAGC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_012961762.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817216.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X4, mRNA.

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CloneID	OXa33041
Clone ID Related Accession (Same CDS sequence)	XM_012961762.2
Accession Version	XM_012961762.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961762.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAATTGCC CGCTGGATGG GAGAAAATTG AAGACCCGGT TTATGGAGTC 
TACTATGTAG ATCACATAAA CAAGAAAACG CAGTATGAAA ATCCTGTATT GGAGGCCAAA 
CGAAAGAAAC AGCTTGGACA GCAGCAAAGT GAAGAATGGA CAGGGGACCA TCCAACCATT 
GCTCCGCCCC CCATAATGCT TCCTAACAAC CTTACTCCAA AAAAGAAGGA ACCAGCAAGA 
GAAACGGCAT CACATGTGAA ACCGTTCTTC ACCAGGAACC CCTCTGAACT GAAAGGCAAG 
TTCATCCATA CGAAGCTAAG GAAAAGTAGT CGGGGCTTTG GCTTTACCGT TGTCGGAGGG 
GACGAACCAG ATGAGTTCTT GCAAATAAAA AGTTTGGTCG TCGACGGTCC GGCCGCGTTG 
GACGGCAAAA TGGAAACGGG TGATGTTATC GTTAGCGTGA ACGATATCTG CGTCCTTGGC 
TACACGCACG CTCAAGTTGT GAAGATCTTC CAGTCTATAC CCATTGGGGA AAGTGTGGAC 
CTTGAACTAT GCCGGGGTTA TCCTCTTCCG TTTGACCCAG ATGACCCCAA CACCAGCCTT 
GTGACATCAG TTGCAATATT GGACAAAGAC CCTATCATTG TCAATGGGCA AGAGAATTAT 
GACTCTCCGT CGAGCAACAG CAGTAAAACT AGAAAAGCCA ATAACGTCAA GGATCCAAGG 
CCTAGCAGCC CTGCTGACAT TTCTTCAAAT GGTTCCCATG GTTACCCTAA TGATACAGTG 
TCCTTGGCAT CCTCTATAGC TACGCAACCT GAACTAATTA CTGTGCATAT AGTAAAAGGA 
CCAATGGGAT TTGGCTTTAC CATAGCAGAC AGTCCTGGCG GTGGGGGCCA GAGAGTGAAG 
CAGATTGTAG ATAGTCCAAG ATGCCGGGGC CTCAAAGAAG GAGATCTTAT AGTAGAGGTG 
AATAAAAAGA ATGTTCAGAA CCTTACCCAT AACCAAGTTG TGGATTTACT CATCGAATGT 
CCTAAGGGAA GCGAAGTTAC CCTACTGGTT CAAAGAGGAG GACTCCCGGT CCCAAAGAAG 
AGCCCAAAGT CGCAACCACT TGAAAGGAAA GACAGCCAAA ACAGCTCGCA GCACAGTGTT 
TCCAGCCAGC ACAGTGGGCA CACTGCTTCA CCCCATCACG CCGCGCCTGG GCTCCCCGAT 
TATCCTACCA GCGAGGCGCC GGCTGTCGAC CAAGCCGACA ACTCCGGGCA GAAAAAGCCT 
GATCCTTTCA AAATCTGGGC CCAATCCAGG AGCATGTATG AGAACAGACT TCCAGACTTT 
CAAGAGCATG ATATATTTTT GTGGCGAAAG GAAACTGGAT TTGGGTTTCG AATTCTCGGT 
GGAAATGAGC CTGGAGAACC AATCTTCATA GGTCATATTG TGCCACTGGG GTCAGCGGAT 
GCCGACGGGC GGTTGCGCTC TGGTGACGAG CTTATCTGTG TGGATGGAAC AGCGGTTGTT 
GGAAAATCTC ATCAACTCGT CGTACAGCTT ATGCAACAGG CTGCGAAGCA AGGACACGTC 
AATCTAACGA TCAGACGAAA AACGATCTAT CCGGTTCCAA AGGCAGAGAA CGAAGCTCCT 
GCATCACCAG CATCCTCGCA CCATAGCAGC ACCCAGCCGG CGTCTCTAAC GGAGGAGAAG 
CGGACGCCCC AAGGCAGCCA AAACTCCTTA AACACTGTCA GTTCAGGCAG CGGCAGCACC 
AGCGGCATTG GCAGTGGCGG AGGCGGCGGG AGCGGCAACG TCAGCACCGT GGTTCAGCCG 
TATGATGTCG AGATCCGACG CGGTGAAAAT GAAGGATTTG GATTTGTCAT TGTGTCTTCT 
GTCAGCCGGC CAGAAGCCGG GACTACTTTT GCCGGCAATG CATGTGTGGC CATGCCTCAC 
AAAATAGGTC GGATAATAGA GGGGAGCCCC GCGGATCGCT GCGGCAAGCT GAAAGTGGGG 
GACCGGATCT TGGCCGTTAA CGGGTGCTCC ATCACCAACA AGTCACACTC GGACATCGTC 
AATTTAATAA AGGAAGCGGG GAACACCGTG ACTCTGCGCA TCATTCCCGG CGATGAAACG 
TCCAACGCGT CTTTATTAAC CAATGCAGAG AAGATTGCCA CGATTACGAC CACGCATACG 
CCACAAACGA TACCCCAAGA AACAAAAAAT AATGCAAAAC CAAAGCCGGA GCCCCAGTTT 
GAATTCAAGA ACTCACCAGC CACACAGCCT GATCAAGAAA TTTATTCAGT GGAACTAGAC 
CGAGGATTAA AAGGATTTGG GTTTAGTCTA CGAGGAGGTC GAGAATATAA CATGGATCTC 
TATGTTTTAC GCCTTGCGGA GGATGGGCCT GCAGCCAGAT GTGGGAAAAT GAGGGTTGGC 
GATGAAATCC TGGAAATAAA CGGAGAGACC ACAAAGAACA TGAAGCACGC CCGGGCAATA 
GAACTGATTA AAAATGGTGG CCGAAGAGTA CGCCTGTGTC TGAAACGCGG AGATGGTTCC 
GTACCGGAAT ATGAACCCGG CGTTGACGGA AACAGCCCCA CAACCAGTCC ACAAAGTGTA 
TCGAAAATGA ACTCGTTACC ATCTGATAGA AATCACTCAT CACTGGAGTT CAATTATCCA 
TCCGATCTTC ACAAATCATT ACAGCATGGA GACAAACGAG CTTACGTTAA AGACCCTAAA 
GGCAACAGGG AGTATGGCAG ACCACATAAC GAACATCACA CTTGGAATGG ATCTTCTAAA 
AATTATTCTG TACAAAATAA CAGAAAAGAT CGATCACCTG AGGGGAGGCG ACCAAGACAG 
GCCGATCGAA CAATGACGAA CGGTCAGAAA AGACAGTCTC CCGAGAAAAG AAATGAAGGA 
ACTAGGAGTG CTGAGAACAC ACTGGAAAGG AGAGAGAAAA ATGAGAGAAG AAAAGAGGTT 
TCTCCTCAAA AGCCCAGGGA AAGGTCGCCG GTGCGCAGAA GGGATGGTTC CCCAACCAGG 
CGAAGGAAAT CCTTGGATAG ATTTATGGAT CAAAGACGAT CCCCTGATAG GAGAAGAGAG 
AGTTCACCTG ACCGAAGAAG AGCAAAGTCA ACGGACAGGA GACGAGAAAG GTCACCAGAA 
AGGAGGAGAG AAAGGTCTCC TGAGAGAAGA AGAGAGAAGT CCACTGAGAG AAGAAGGGAC 
AGGTCTCCTG ATAAACGAAA CAAAGAGCAA CGAACCAGCA ACAGAGACAG GGATGAACAC 
AGTCTGAAAT ATGATACCCT CAGAAGTGGC AGGCACCAAC AAGAACAACG GAGGAGGCAA 
TACAAGGAAT GCAGCACCGA CCTGAGCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817216.1
CDS	284..4636
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_012961762.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961762.2

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAATTGCC CGCTGGATGG GAGAAAATTG AAGACCCGGT TTATGGAGTC 
TACTATGTAG ATCACATAAA CAAGAAAACG CAGTATGAAA ATCCTGTATT GGAGGCCAAA 
CGAAAGAAAC AGCTTGGACA GCAGCAAAGT GAAGAATGGA CAGGGGACCA TCCAACCATT 
GCTCCGCCCC CCATAATGCT TCCTAACAAC CTTACTCCAA AAAAGAAGGA ACCAGCAAGA 
GAAACGGCAT CACATGTGAA ACCGTTCTTC ACCAGGAACC CCTCTGAACT GAAAGGCAAG 
TTCATCCATA CGAAGCTAAG GAAAAGTAGT CGGGGCTTTG GCTTTACCGT TGTCGGAGGG 
GACGAACCAG ATGAGTTCTT GCAAATAAAA AGTTTGGTCG TCGACGGTCC GGCCGCGTTG 
GACGGCAAAA TGGAAACGGG TGATGTTATC GTTAGCGTGA ACGATATCTG CGTCCTTGGC 
TACACGCACG CTCAAGTTGT GAAGATCTTC CAGTCTATAC CCATTGGGGA AAGTGTGGAC 
CTTGAACTAT GCCGGGGTTA TCCTCTTCCG TTTGACCCAG ATGACCCCAA CACCAGCCTT 
GTGACATCAG TTGCAATATT GGACAAAGAC CCTATCATTG TCAATGGGCA AGAGAATTAT 
GACTCTCCGT CGAGCAACAG CAGTAAAACT AGAAAAGCCA ATAACGTCAA GGATCCAAGG 
CCTAGCAGCC CTGCTGACAT TTCTTCAAAT GGTTCCCATG GTTACCCTAA TGATACAGTG 
TCCTTGGCAT CCTCTATAGC TACGCAACCT GAACTAATTA CTGTGCATAT AGTAAAAGGA 
CCAATGGGAT TTGGCTTTAC CATAGCAGAC AGTCCTGGCG GTGGGGGCCA GAGAGTGAAG 
CAGATTGTAG ATAGTCCAAG ATGCCGGGGC CTCAAAGAAG GAGATCTTAT AGTAGAGGTG 
AATAAAAAGA ATGTTCAGAA CCTTACCCAT AACCAAGTTG TGGATTTACT CATCGAATGT 
CCTAAGGGAA GCGAAGTTAC CCTACTGGTT CAAAGAGGAG GACTCCCGGT CCCAAAGAAG 
AGCCCAAAGT CGCAACCACT TGAAAGGAAA GACAGCCAAA ACAGCTCGCA GCACAGTGTT 
TCCAGCCAGC ACAGTGGGCA CACTGCTTCA CCCCATCACG CCGCGCCTGG GCTCCCCGAT 
TATCCTACCA GCGAGGCGCC GGCTGTCGAC CAAGCCGACA ACTCCGGGCA GAAAAAGCCT 
GATCCTTTCA AAATCTGGGC CCAATCCAGG AGCATGTATG AGAACAGACT TCCAGACTTT 
CAAGAGCATG ATATATTTTT GTGGCGAAAG GAAACTGGAT TTGGGTTTCG AATTCTCGGT 
GGAAATGAGC CTGGAGAACC AATCTTCATA GGTCATATTG TGCCACTGGG GTCAGCGGAT 
GCCGACGGGC GGTTGCGCTC TGGTGACGAG CTTATCTGTG TGGATGGAAC AGCGGTTGTT 
GGAAAATCTC ATCAACTCGT CGTACAGCTT ATGCAACAGG CTGCGAAGCA AGGACACGTC 
AATCTAACGA TCAGACGAAA AACGATCTAT CCGGTTCCAA AGGCAGAGAA CGAAGCTCCT 
GCATCACCAG CATCCTCGCA CCATAGCAGC ACCCAGCCGG CGTCTCTAAC GGAGGAGAAG 
CGGACGCCCC AAGGCAGCCA AAACTCCTTA AACACTGTCA GTTCAGGCAG CGGCAGCACC 
AGCGGCATTG GCAGTGGCGG AGGCGGCGGG AGCGGCAACG TCAGCACCGT GGTTCAGCCG 
TATGATGTCG AGATCCGACG CGGTGAAAAT GAAGGATTTG GATTTGTCAT TGTGTCTTCT 
GTCAGCCGGC CAGAAGCCGG GACTACTTTT GCCGGCAATG CATGTGTGGC CATGCCTCAC 
AAAATAGGTC GGATAATAGA GGGGAGCCCC GCGGATCGCT GCGGCAAGCT GAAAGTGGGG 
GACCGGATCT TGGCCGTTAA CGGGTGCTCC ATCACCAACA AGTCACACTC GGACATCGTC 
AATTTAATAA AGGAAGCGGG GAACACCGTG ACTCTGCGCA TCATTCCCGG CGATGAAACG 
TCCAACGCGT CTTTATTAAC CAATGCAGAG AAGATTGCCA CGATTACGAC CACGCATACG 
CCACAAACGA TACCCCAAGA AACAAAAAAT AATGCAAAAC CAAAGCCGGA GCCCCAGTTT 
GAATTCAAGA ACTCACCAGC CACACAGCCT GATCAAGAAA TTTATTCAGT GGAACTAGAC 
CGAGGATTAA AAGGATTTGG GTTTAGTCTA CGAGGAGGTC GAGAATATAA CATGGATCTC 
TATGTTTTAC GCCTTGCGGA GGATGGGCCT GCAGCCAGAT GTGGGAAAAT GAGGGTTGGC 
GATGAAATCC TGGAAATAAA CGGAGAGACC ACAAAGAACA TGAAGCACGC CCGGGCAATA 
GAACTGATTA AAAATGGTGG CCGAAGAGTA CGCCTGTGTC TGAAACGCGG AGATGGTTCC 
GTACCGGAAT ATGAACCCGG CGTTGACGGA AACAGCCCCA CAACCAGTCC ACAAAGTGTA 
TCGAAAATGA ACTCGTTACC ATCTGATAGA AATCACTCAT CACTGGAGTT CAATTATCCA 
TCCGATCTTC ACAAATCATT ACAGCATGGA GACAAACGAG CTTACGTTAA AGACCCTAAA 
GGCAACAGGG AGTATGGCAG ACCACATAAC GAACATCACA CTTGGAATGG ATCTTCTAAA 
AATTATTCTG TACAAAATAA CAGAAAAGAT CGATCACCTG AGGGGAGGCG ACCAAGACAG 
GCCGATCGAA CAATGACGAA CGGTCAGAAA AGACAGTCTC CCGAGAAAAG AAATGAAGGA 
ACTAGGAGTG CTGAGAACAC ACTGGAAAGG AGAGAGAAAA ATGAGAGAAG AAAAGAGGTT 
TCTCCTCAAA AGCCCAGGGA AAGGTCGCCG GTGCGCAGAA GGGATGGTTC CCCAACCAGG 
CGAAGGAAAT CCTTGGATAG ATTTATGGAT CAAAGACGAT CCCCTGATAG GAGAAGAGAG 
AGTTCACCTG ACCGAAGAAG AGCAAAGTCA ACGGACAGGA GACGAGAAAG GTCACCAGAA 
AGGAGGAGAG AAAGGTCTCC TGAGAGAAGA AGAGAGAAGT CCACTGAGAG AAGAAGGGAC 
AGGTCTCCTG ATAAACGAAA CAAAGAGCAA CGAACCAGCA ACAGAGACAG GGATGAACAC 
AGTCTGAAAT ATGATACCCT CAGAAGTGGC AGGCACCAAC AAGAACAACG GAGGAGGCAA 
TACAAGGAAT GCAGCACCGA CCTGAGCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_012961763.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817217.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X5, mRNA.

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CloneID	OXa33042
Clone ID Related Accession (Same CDS sequence)	XM_012961763.2 , XM_018089383.1
Accession Version	XM_012961763.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961763.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG CAGGCGTAGT GCATACCGAC CACTATGAGT TTGATGATGA TGCTCCTGAA 
ATGAGCAGTA GCTTTACAGG AGACTCCAGT GAAGCAGACG AGCACACGAT TAACCTTTCC 
CAAGATAAAG TCCCACCGGC GGCTCGGATA CTCCCTACTT TTCCCATCAC GGAAGCTTCT 
CAGAATCTGC CTCATTACCT ACCTCCTGAC GAGGAAAGCC TTGGACCTCT ACCTGATAAC 
TGGGAAATGG CCTTTACAGA GAATGGAGAA GTCTATTTTA TAGACCATAA CACTAAAACG 
ACATCTTGGT TAGACCCCCG GTGCCTGAAC AAACAGCAGA AGCCTCTGGA AGAATGTGAA 
GATGATGAAG AAGGGGTACA CACCGAGGAA CTGGACAGTG AACTAGAATT GCCCGCTGGA 
TGGGAGAAAA TTGAAGACCC GGTTTATGGA GTCTACTATG TAGATCACAT AAACAAGAAA 
ACGCAGTATG AAAATCCTGT ATTGGAGGCC AAACGAAAGA AACAGCTTGG ACAGCAGCAA 
AGTGAAGAAT GGACAGGGGA CCATCCAACC ATTGCTCCGC CCCCCATAAT GCTTCCTAAC 
AACCTTACTC CAAAAAAGAA GGAACCAGCA AGAGAAACGG CATCACATGT GAAACCGTTC 
TTCACCAGGA ACCCCTCTGA ACTGAAAGGC AAGTTCATCC ATACGAAGCT AAGGAAAAGT 
AGTCGGGGCT TTGGCTTTAC CGTTGTCGGA GGGGACGAAC CAGATGAGTT CTTGCAAATA 
AAAAGTTTGG TCGTCGACGG TCCGGCCGCG TTGGACGGCA AAATGGAAAC GGGTGATGTT 
ATCGTTAGCG TGAACGATAT CTGCGTCCTT GGCTACACGC ACGCTCAAGT TGTGAAGATC 
TTCCAGTCTA TACCCATTGG GGAAAGTGTG GACCTTGAAC TATGCCGGGG TTATCCTCTT 
CCGTTTGACC CAGATGACCC CAACACCAGC CTTGTGACAT CAGTTGCAAT ATTGGACAAA 
GACCCTATCA TTGTCAATGG GCAAGAGAAT TATGACTCTC CGTCGAGCAA CAGCAGTAAA 
ACTAGAAAAG CCAATAACGT CAAGGATCCA AGGCCTAGCA GCCCTGCTGA CATTTCTTCA 
AATGGTTCCC ATGGTTACCC TAATGATACA GTGTCCTTGG CATCCTCTAT AGCTACGCAA 
CCTGAACTAA TTACTGTGCA TATAGTAAAA GGACCAATGG GATTTGGCTT TACCATAGCA 
GACAGTCCTG GCGGTGGGGG CCAGAGAGTG AAGCAGATTG TAGATAGTCC AAGATGCCGG 
GGCCTCAAAG AAGGAGATCT TATAGTAGAG GTGAATAAAA AGAATGTTCA GAACCTTACC 
CATAACCAAG TTGTGGATTT ACTCATCGAA TGTCCTAAGG GAAGCGAAGT TACCCTACTG 
GTTCAAAGAG GAGGACTCCC GGTCCCAAAG AAGAGCCCAA AGTCGCAACC ACTTGAAAGG 
AAAGACAGCC AAAACAGCTC GCAGCACAGT GTTTCCAGCC AGCACAGTGG GCACACTGCT 
TCACCCCATC ACGCCGCGCC TGGGCTCCCC GATTATCCTA CCAGCGAGGC GCCGGCTGTC 
GACCAAGCCG ACAACTCCGG GCAGAAAAAG CCTGATCCTT TCAAAATCTG GGCCCAATCC 
AGGAGCATGT ATGAGAACAG ACTTCCAGAC TTTCAAGAGC ATGATATATT TTTGTGGCGA 
AAGGAAACTG GATTTGGGTT TCGAATTCTC GGTGGAAATG AGCCTGGAGA ACCAATCTTC 
ATAGGTCATA TTGTGCCACT GGGGTCAGCG GATGCCGACG GGCGGTTGCG CTCTGGTGAC 
GAGCTTATCT GTGTGGATGG AACAGCGGTT GTTGGAAAAT CTCATCAACT CGTCGTACAG 
CTTATGCAAC AGGCTGCGAA GCAAGGACAC GTCAATCTAA CGATCAGACG AAAAACGATC 
TATCCGGTTC CAAAGGCAGA GAACGAAGCT CCTGCATCAC CAGCATCCTC GCACCATAGC 
AGCACCCAGC CGGCGTCTCT AACGGAGGAG AAGCGGACGC CCCAAGGCAG CCAAAACTCC 
TTAAACACTG TCAGTTCAGG CAGCGGCAGC ACCAGCGGCA TTGGCAGTGG CGGAGGCGGC 
GGGAGCGGCA ACGTCAGCAC CGTGGTTCAG CCGTATGATG TCGAGATCCG ACGCGGTGAA 
AATGAAGGAT TTGGATTTGT CATTGTGTCT TCTGTCAGCC GGCCAGAAGC CGGGACTACT 
TTTGCCGGCA ATGCATGTGT GGCCATGCCT CACAAAATAG GTCGGATAAT AGAGGGGAGC 
CCCGCGGATC GCTGCGGCAA GCTGAAAGTG GGGGACCGGA TCTTGGCCGT TAACGGGTGC 
TCCATCACCA ACAAGTCACA CTCGGACATC GTCAATTTAA TAAAGGAAGC GGGGAACACC 
GTGACTCTGC GCATCATTCC CGGCGATGAA ACGTCCAACG CGTCTTTATT AACCAATGCA 
GAGAAGATTG CCACGATTAC GACCACGCAT ACGCCACAAA CGATACCCCA AGAAACAAAA 
AATAATGCAA AACCAAAGCC GGAGCCCCAG TTTGAATTCA AGAACTCACC AGCCACACAG 
CCTGATCAAG AAATTTATTC AGTGGAACTA GACCGAGGAT TAAAAGGATT TGGGTTTAGT 
CTACGAGGAG GTCGAGAATA TAACATGGAT CTCTATGTTT TACGCCTTGC GGAGGATGGG 
CCTGCAGCCA GATGTGGGAA AATGAGGGTT GGCGATGAAA TCCTGGAAAT AAACGGAGAG 
ACCACAAAGA ACATGAAGCA CGCCCGGGCA ATAGAACTGA TTAAAAATGG TGGCCGAAGA 
GTACGCCTGT GTCTGAAACG CGGAGATGGT TCCGTACCGG AATATGAACC CGGCGTTGAC 
GGAAACAGCC CCACAACCAG TCCACAAAGT GTATCGAAAA TGAACTCGTT ACCATCTGAT 
AGAAATCACT CATCACTGGA GTTCAATTAT CCATCCGATC TTCACAAATC ATTACAGCAT 
GGAGACAAAC GAGCTTACGT TAAAGACCCT AAAGGCAACA GGGAGTATGG CAGACCACAT 
AACGAACATC ACACTTGGAA TGGATCTTCT AAAAATTATT CTGTACAAAA TAACAGAAAA 
GATCGATCAC CTGAGGGGAG GCGACCAAGA CAGGCCGATC GAACAATGAC GAACGGTCAG 
AAAAGACAGT CTCCCGAGAA AAGAAATGAA GGAACTAGGA GTGCTGAGAA CACACTGGAA 
AGGAGAGAGA AAAATGAGAG AAGAAAAGAG GTTTCTCCTC AAAAGCCCAG GGAAAGGTCG 
CCGGTGCGCA GAAGGGATGG TTCCCCAACC AGGCGAAGGA AATCCTTGGA TAGATTTATG 
GATCAAAGAC GATCCCCTGA TAGGAGAAGA GAGAGTTCAC CTGACCGAAG AAGAGCAAAG 
TCAACGGACA GGAGACGAGA AAGGTCACCA GAAAGGAGGA GAGAAAGGTC TCCTGAGAGA 
AGAAGAGAGA AGTCCACTGA GAGAAGAAGG GACAGGTCTC CTGATAAACG AAACAAAGAG 
CAACGAACCA GCAACAGAGA CAGGGATGAA CACAGTCTGA AATATGATAC CCTCAGAAGT 
GGCAGGCACC AACAAGAACA ACGGAGGAGG CAATACAAGG AATGCAGCAC CGACCTGAGC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817217.1
CDS	495..4220
Translation	MQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_012961763.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961763.2

ORF Insert Sequence:

ATGCAAAATG CAGGCGTAGT GCATACCGAC CACTATGAGT TTGATGATGA TGCTCCTGAA 
ATGAGCAGTA GCTTTACAGG AGACTCCAGT GAAGCAGACG AGCACACGAT TAACCTTTCC 
CAAGATAAAG TCCCACCGGC GGCTCGGATA CTCCCTACTT TTCCCATCAC GGAAGCTTCT 
CAGAATCTGC CTCATTACCT ACCTCCTGAC GAGGAAAGCC TTGGACCTCT ACCTGATAAC 
TGGGAAATGG CCTTTACAGA GAATGGAGAA GTCTATTTTA TAGACCATAA CACTAAAACG 
ACATCTTGGT TAGACCCCCG GTGCCTGAAC AAACAGCAGA AGCCTCTGGA AGAATGTGAA 
GATGATGAAG AAGGGGTACA CACCGAGGAA CTGGACAGTG AACTAGAATT GCCCGCTGGA 
TGGGAGAAAA TTGAAGACCC GGTTTATGGA GTCTACTATG TAGATCACAT AAACAAGAAA 
ACGCAGTATG AAAATCCTGT ATTGGAGGCC AAACGAAAGA AACAGCTTGG ACAGCAGCAA 
AGTGAAGAAT GGACAGGGGA CCATCCAACC ATTGCTCCGC CCCCCATAAT GCTTCCTAAC 
AACCTTACTC CAAAAAAGAA GGAACCAGCA AGAGAAACGG CATCACATGT GAAACCGTTC 
TTCACCAGGA ACCCCTCTGA ACTGAAAGGC AAGTTCATCC ATACGAAGCT AAGGAAAAGT 
AGTCGGGGCT TTGGCTTTAC CGTTGTCGGA GGGGACGAAC CAGATGAGTT CTTGCAAATA 
AAAAGTTTGG TCGTCGACGG TCCGGCCGCG TTGGACGGCA AAATGGAAAC GGGTGATGTT 
ATCGTTAGCG TGAACGATAT CTGCGTCCTT GGCTACACGC ACGCTCAAGT TGTGAAGATC 
TTCCAGTCTA TACCCATTGG GGAAAGTGTG GACCTTGAAC TATGCCGGGG TTATCCTCTT 
CCGTTTGACC CAGATGACCC CAACACCAGC CTTGTGACAT CAGTTGCAAT ATTGGACAAA 
GACCCTATCA TTGTCAATGG GCAAGAGAAT TATGACTCTC CGTCGAGCAA CAGCAGTAAA 
ACTAGAAAAG CCAATAACGT CAAGGATCCA AGGCCTAGCA GCCCTGCTGA CATTTCTTCA 
AATGGTTCCC ATGGTTACCC TAATGATACA GTGTCCTTGG CATCCTCTAT AGCTACGCAA 
CCTGAACTAA TTACTGTGCA TATAGTAAAA GGACCAATGG GATTTGGCTT TACCATAGCA 
GACAGTCCTG GCGGTGGGGG CCAGAGAGTG AAGCAGATTG TAGATAGTCC AAGATGCCGG 
GGCCTCAAAG AAGGAGATCT TATAGTAGAG GTGAATAAAA AGAATGTTCA GAACCTTACC 
CATAACCAAG TTGTGGATTT ACTCATCGAA TGTCCTAAGG GAAGCGAAGT TACCCTACTG 
GTTCAAAGAG GAGGACTCCC GGTCCCAAAG AAGAGCCCAA AGTCGCAACC ACTTGAAAGG 
AAAGACAGCC AAAACAGCTC GCAGCACAGT GTTTCCAGCC AGCACAGTGG GCACACTGCT 
TCACCCCATC ACGCCGCGCC TGGGCTCCCC GATTATCCTA CCAGCGAGGC GCCGGCTGTC 
GACCAAGCCG ACAACTCCGG GCAGAAAAAG CCTGATCCTT TCAAAATCTG GGCCCAATCC 
AGGAGCATGT ATGAGAACAG ACTTCCAGAC TTTCAAGAGC ATGATATATT TTTGTGGCGA 
AAGGAAACTG GATTTGGGTT TCGAATTCTC GGTGGAAATG AGCCTGGAGA ACCAATCTTC 
ATAGGTCATA TTGTGCCACT GGGGTCAGCG GATGCCGACG GGCGGTTGCG CTCTGGTGAC 
GAGCTTATCT GTGTGGATGG AACAGCGGTT GTTGGAAAAT CTCATCAACT CGTCGTACAG 
CTTATGCAAC AGGCTGCGAA GCAAGGACAC GTCAATCTAA CGATCAGACG AAAAACGATC 
TATCCGGTTC CAAAGGCAGA GAACGAAGCT CCTGCATCAC CAGCATCCTC GCACCATAGC 
AGCACCCAGC CGGCGTCTCT AACGGAGGAG AAGCGGACGC CCCAAGGCAG CCAAAACTCC 
TTAAACACTG TCAGTTCAGG CAGCGGCAGC ACCAGCGGCA TTGGCAGTGG CGGAGGCGGC 
GGGAGCGGCA ACGTCAGCAC CGTGGTTCAG CCGTATGATG TCGAGATCCG ACGCGGTGAA 
AATGAAGGAT TTGGATTTGT CATTGTGTCT TCTGTCAGCC GGCCAGAAGC CGGGACTACT 
TTTGCCGGCA ATGCATGTGT GGCCATGCCT CACAAAATAG GTCGGATAAT AGAGGGGAGC 
CCCGCGGATC GCTGCGGCAA GCTGAAAGTG GGGGACCGGA TCTTGGCCGT TAACGGGTGC 
TCCATCACCA ACAAGTCACA CTCGGACATC GTCAATTTAA TAAAGGAAGC GGGGAACACC 
GTGACTCTGC GCATCATTCC CGGCGATGAA ACGTCCAACG CGTCTTTATT AACCAATGCA 
GAGAAGATTG CCACGATTAC GACCACGCAT ACGCCACAAA CGATACCCCA AGAAACAAAA 
AATAATGCAA AACCAAAGCC GGAGCCCCAG TTTGAATTCA AGAACTCACC AGCCACACAG 
CCTGATCAAG AAATTTATTC AGTGGAACTA GACCGAGGAT TAAAAGGATT TGGGTTTAGT 
CTACGAGGAG GTCGAGAATA TAACATGGAT CTCTATGTTT TACGCCTTGC GGAGGATGGG 
CCTGCAGCCA GATGTGGGAA AATGAGGGTT GGCGATGAAA TCCTGGAAAT AAACGGAGAG 
ACCACAAAGA ACATGAAGCA CGCCCGGGCA ATAGAACTGA TTAAAAATGG TGGCCGAAGA 
GTACGCCTGT GTCTGAAACG CGGAGATGGT TCCGTACCGG AATATGAACC CGGCGTTGAC 
GGAAACAGCC CCACAACCAG TCCACAAAGT GTATCGAAAA TGAACTCGTT ACCATCTGAT 
AGAAATCACT CATCACTGGA GTTCAATTAT CCATCCGATC TTCACAAATC ATTACAGCAT 
GGAGACAAAC GAGCTTACGT TAAAGACCCT AAAGGCAACA GGGAGTATGG CAGACCACAT 
AACGAACATC ACACTTGGAA TGGATCTTCT AAAAATTATT CTGTACAAAA TAACAGAAAA 
GATCGATCAC CTGAGGGGAG GCGACCAAGA CAGGCCGATC GAACAATGAC GAACGGTCAG 
AAAAGACAGT CTCCCGAGAA AAGAAATGAA GGAACTAGGA GTGCTGAGAA CACACTGGAA 
AGGAGAGAGA AAAATGAGAG AAGAAAAGAG GTTTCTCCTC AAAAGCCCAG GGAAAGGTCG 
CCGGTGCGCA GAAGGGATGG TTCCCCAACC AGGCGAAGGA AATCCTTGGA TAGATTTATG 
GATCAAAGAC GATCCCCTGA TAGGAGAAGA GAGAGTTCAC CTGACCGAAG AAGAGCAAAG 
TCAACGGACA GGAGACGAGA AAGGTCACCA GAAAGGAGGA GAGAAAGGTC TCCTGAGAGA 
AGAAGAGAGA AGTCCACTGA GAGAAGAAGG GACAGGTCTC CTGATAAACG AAACAAAGAG 
CAACGAACCA GCAACAGAGA CAGGGATGAA CACAGTCTGA AATATGATAC CCTCAGAAGT 
GGCAGGCACC AACAAGAACA ACGGAGGAGG CAATACAAGG AATGCAGCAC CGACCTGAGC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_018089383.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017944872.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa33042
Clone ID Related Accession (Same CDS sequence)	XM_012961763.2 , XM_018089383.1
Accession Version	XM_018089383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG CAGGCGTAGT GCATACCGAC CACTATGAGT TTGATGATGA TGCTCCTGAA 
ATGAGCAGTA GCTTTACAGG AGACTCCAGT GAAGCAGACG AGCACACGAT TAACCTTTCC 
CAAGATAAAG TCCCACCGGC GGCTCGGATA CTCCCTACTT TTCCCATCAC GGAAGCTTCT 
CAGAATCTGC CTCATTACCT ACCTCCTGAC GAGGAAAGCC TTGGACCTCT ACCTGATAAC 
TGGGAAATGG CCTTTACAGA GAATGGAGAA GTCTATTTTA TAGACCATAA CACTAAAACG 
ACATCTTGGT TAGACCCCCG GTGCCTGAAC AAACAGCAGA AGCCTCTGGA AGAATGTGAA 
GATGATGAAG AAGGGGTACA CACCGAGGAA CTGGACAGTG AACTAGAATT GCCCGCTGGA 
TGGGAGAAAA TTGAAGACCC GGTTTATGGA GTCTACTATG TAGATCACAT AAACAAGAAA 
ACGCAGTATG AAAATCCTGT ATTGGAGGCC AAACGAAAGA AACAGCTTGG ACAGCAGCAA 
AGTGAAGAAT GGACAGGGGA CCATCCAACC ATTGCTCCGC CCCCCATAAT GCTTCCTAAC 
AACCTTACTC CAAAAAAGAA GGAACCAGCA AGAGAAACGG CATCACATGT GAAACCGTTC 
TTCACCAGGA ACCCCTCTGA ACTGAAAGGC AAGTTCATCC ATACGAAGCT AAGGAAAAGT 
AGTCGGGGCT TTGGCTTTAC CGTTGTCGGA GGGGACGAAC CAGATGAGTT CTTGCAAATA 
AAAAGTTTGG TCGTCGACGG TCCGGCCGCG TTGGACGGCA AAATGGAAAC GGGTGATGTT 
ATCGTTAGCG TGAACGATAT CTGCGTCCTT GGCTACACGC ACGCTCAAGT TGTGAAGATC 
TTCCAGTCTA TACCCATTGG GGAAAGTGTG GACCTTGAAC TATGCCGGGG TTATCCTCTT 
CCGTTTGACC CAGATGACCC CAACACCAGC CTTGTGACAT CAGTTGCAAT ATTGGACAAA 
GACCCTATCA TTGTCAATGG GCAAGAGAAT TATGACTCTC CGTCGAGCAA CAGCAGTAAA 
ACTAGAAAAG CCAATAACGT CAAGGATCCA AGGCCTAGCA GCCCTGCTGA CATTTCTTCA 
AATGGTTCCC ATGGTTACCC TAATGATACA GTGTCCTTGG CATCCTCTAT AGCTACGCAA 
CCTGAACTAA TTACTGTGCA TATAGTAAAA GGACCAATGG GATTTGGCTT TACCATAGCA 
GACAGTCCTG GCGGTGGGGG CCAGAGAGTG AAGCAGATTG TAGATAGTCC AAGATGCCGG 
GGCCTCAAAG AAGGAGATCT TATAGTAGAG GTGAATAAAA AGAATGTTCA GAACCTTACC 
CATAACCAAG TTGTGGATTT ACTCATCGAA TGTCCTAAGG GAAGCGAAGT TACCCTACTG 
GTTCAAAGAG GAGGACTCCC GGTCCCAAAG AAGAGCCCAA AGTCGCAACC ACTTGAAAGG 
AAAGACAGCC AAAACAGCTC GCAGCACAGT GTTTCCAGCC AGCACAGTGG GCACACTGCT 
TCACCCCATC ACGCCGCGCC TGGGCTCCCC GATTATCCTA CCAGCGAGGC GCCGGCTGTC 
GACCAAGCCG ACAACTCCGG GCAGAAAAAG CCTGATCCTT TCAAAATCTG GGCCCAATCC 
AGGAGCATGT ATGAGAACAG ACTTCCAGAC TTTCAAGAGC ATGATATATT TTTGTGGCGA 
AAGGAAACTG GATTTGGGTT TCGAATTCTC GGTGGAAATG AGCCTGGAGA ACCAATCTTC 
ATAGGTCATA TTGTGCCACT GGGGTCAGCG GATGCCGACG GGCGGTTGCG CTCTGGTGAC 
GAGCTTATCT GTGTGGATGG AACAGCGGTT GTTGGAAAAT CTCATCAACT CGTCGTACAG 
CTTATGCAAC AGGCTGCGAA GCAAGGACAC GTCAATCTAA CGATCAGACG AAAAACGATC 
TATCCGGTTC CAAAGGCAGA GAACGAAGCT CCTGCATCAC CAGCATCCTC GCACCATAGC 
AGCACCCAGC CGGCGTCTCT AACGGAGGAG AAGCGGACGC CCCAAGGCAG CCAAAACTCC 
TTAAACACTG TCAGTTCAGG CAGCGGCAGC ACCAGCGGCA TTGGCAGTGG CGGAGGCGGC 
GGGAGCGGCA ACGTCAGCAC CGTGGTTCAG CCGTATGATG TCGAGATCCG ACGCGGTGAA 
AATGAAGGAT TTGGATTTGT CATTGTGTCT TCTGTCAGCC GGCCAGAAGC CGGGACTACT 
TTTGCCGGCA ATGCATGTGT GGCCATGCCT CACAAAATAG GTCGGATAAT AGAGGGGAGC 
CCCGCGGATC GCTGCGGCAA GCTGAAAGTG GGGGACCGGA TCTTGGCCGT TAACGGGTGC 
TCCATCACCA ACAAGTCACA CTCGGACATC GTCAATTTAA TAAAGGAAGC GGGGAACACC 
GTGACTCTGC GCATCATTCC CGGCGATGAA ACGTCCAACG CGTCTTTATT AACCAATGCA 
GAGAAGATTG CCACGATTAC GACCACGCAT ACGCCACAAA CGATACCCCA AGAAACAAAA 
AATAATGCAA AACCAAAGCC GGAGCCCCAG TTTGAATTCA AGAACTCACC AGCCACACAG 
CCTGATCAAG AAATTTATTC AGTGGAACTA GACCGAGGAT TAAAAGGATT TGGGTTTAGT 
CTACGAGGAG GTCGAGAATA TAACATGGAT CTCTATGTTT TACGCCTTGC GGAGGATGGG 
CCTGCAGCCA GATGTGGGAA AATGAGGGTT GGCGATGAAA TCCTGGAAAT AAACGGAGAG 
ACCACAAAGA ACATGAAGCA CGCCCGGGCA ATAGAACTGA TTAAAAATGG TGGCCGAAGA 
GTACGCCTGT GTCTGAAACG CGGAGATGGT TCCGTACCGG AATATGAACC CGGCGTTGAC 
GGAAACAGCC CCACAACCAG TCCACAAAGT GTATCGAAAA TGAACTCGTT ACCATCTGAT 
AGAAATCACT CATCACTGGA GTTCAATTAT CCATCCGATC TTCACAAATC ATTACAGCAT 
GGAGACAAAC GAGCTTACGT TAAAGACCCT AAAGGCAACA GGGAGTATGG CAGACCACAT 
AACGAACATC ACACTTGGAA TGGATCTTCT AAAAATTATT CTGTACAAAA TAACAGAAAA 
GATCGATCAC CTGAGGGGAG GCGACCAAGA CAGGCCGATC GAACAATGAC GAACGGTCAG 
AAAAGACAGT CTCCCGAGAA AAGAAATGAA GGAACTAGGA GTGCTGAGAA CACACTGGAA 
AGGAGAGAGA AAAATGAGAG AAGAAAAGAG GTTTCTCCTC AAAAGCCCAG GGAAAGGTCG 
CCGGTGCGCA GAAGGGATGG TTCCCCAACC AGGCGAAGGA AATCCTTGGA TAGATTTATG 
GATCAAAGAC GATCCCCTGA TAGGAGAAGA GAGAGTTCAC CTGACCGAAG AAGAGCAAAG 
TCAACGGACA GGAGACGAGA AAGGTCACCA GAAAGGAGGA GAGAAAGGTC TCCTGAGAGA 
AGAAGAGAGA AGTCCACTGA GAGAAGAAGG GACAGGTCTC CTGATAAACG AAACAAAGAG 
CAACGAACCA GCAACAGAGA CAGGGATGAA CACAGTCTGA AATATGATAC CCTCAGAAGT 
GGCAGGCACC AACAAGAACA ACGGAGGAGG CAATACAAGG AATGCAGCAC CGACCTGAGC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017944872.1
CDS	213..3938
Translation	MQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_018089383.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018089383.1

ORF Insert Sequence:

ATGCAAAATG CAGGCGTAGT GCATACCGAC CACTATGAGT TTGATGATGA TGCTCCTGAA 
ATGAGCAGTA GCTTTACAGG AGACTCCAGT GAAGCAGACG AGCACACGAT TAACCTTTCC 
CAAGATAAAG TCCCACCGGC GGCTCGGATA CTCCCTACTT TTCCCATCAC GGAAGCTTCT 
CAGAATCTGC CTCATTACCT ACCTCCTGAC GAGGAAAGCC TTGGACCTCT ACCTGATAAC 
TGGGAAATGG CCTTTACAGA GAATGGAGAA GTCTATTTTA TAGACCATAA CACTAAAACG 
ACATCTTGGT TAGACCCCCG GTGCCTGAAC AAACAGCAGA AGCCTCTGGA AGAATGTGAA 
GATGATGAAG AAGGGGTACA CACCGAGGAA CTGGACAGTG AACTAGAATT GCCCGCTGGA 
TGGGAGAAAA TTGAAGACCC GGTTTATGGA GTCTACTATG TAGATCACAT AAACAAGAAA 
ACGCAGTATG AAAATCCTGT ATTGGAGGCC AAACGAAAGA AACAGCTTGG ACAGCAGCAA 
AGTGAAGAAT GGACAGGGGA CCATCCAACC ATTGCTCCGC CCCCCATAAT GCTTCCTAAC 
AACCTTACTC CAAAAAAGAA GGAACCAGCA AGAGAAACGG CATCACATGT GAAACCGTTC 
TTCACCAGGA ACCCCTCTGA ACTGAAAGGC AAGTTCATCC ATACGAAGCT AAGGAAAAGT 
AGTCGGGGCT TTGGCTTTAC CGTTGTCGGA GGGGACGAAC CAGATGAGTT CTTGCAAATA 
AAAAGTTTGG TCGTCGACGG TCCGGCCGCG TTGGACGGCA AAATGGAAAC GGGTGATGTT 
ATCGTTAGCG TGAACGATAT CTGCGTCCTT GGCTACACGC ACGCTCAAGT TGTGAAGATC 
TTCCAGTCTA TACCCATTGG GGAAAGTGTG GACCTTGAAC TATGCCGGGG TTATCCTCTT 
CCGTTTGACC CAGATGACCC CAACACCAGC CTTGTGACAT CAGTTGCAAT ATTGGACAAA 
GACCCTATCA TTGTCAATGG GCAAGAGAAT TATGACTCTC CGTCGAGCAA CAGCAGTAAA 
ACTAGAAAAG CCAATAACGT CAAGGATCCA AGGCCTAGCA GCCCTGCTGA CATTTCTTCA 
AATGGTTCCC ATGGTTACCC TAATGATACA GTGTCCTTGG CATCCTCTAT AGCTACGCAA 
CCTGAACTAA TTACTGTGCA TATAGTAAAA GGACCAATGG GATTTGGCTT TACCATAGCA 
GACAGTCCTG GCGGTGGGGG CCAGAGAGTG AAGCAGATTG TAGATAGTCC AAGATGCCGG 
GGCCTCAAAG AAGGAGATCT TATAGTAGAG GTGAATAAAA AGAATGTTCA GAACCTTACC 
CATAACCAAG TTGTGGATTT ACTCATCGAA TGTCCTAAGG GAAGCGAAGT TACCCTACTG 
GTTCAAAGAG GAGGACTCCC GGTCCCAAAG AAGAGCCCAA AGTCGCAACC ACTTGAAAGG 
AAAGACAGCC AAAACAGCTC GCAGCACAGT GTTTCCAGCC AGCACAGTGG GCACACTGCT 
TCACCCCATC ACGCCGCGCC TGGGCTCCCC GATTATCCTA CCAGCGAGGC GCCGGCTGTC 
GACCAAGCCG ACAACTCCGG GCAGAAAAAG CCTGATCCTT TCAAAATCTG GGCCCAATCC 
AGGAGCATGT ATGAGAACAG ACTTCCAGAC TTTCAAGAGC ATGATATATT TTTGTGGCGA 
AAGGAAACTG GATTTGGGTT TCGAATTCTC GGTGGAAATG AGCCTGGAGA ACCAATCTTC 
ATAGGTCATA TTGTGCCACT GGGGTCAGCG GATGCCGACG GGCGGTTGCG CTCTGGTGAC 
GAGCTTATCT GTGTGGATGG AACAGCGGTT GTTGGAAAAT CTCATCAACT CGTCGTACAG 
CTTATGCAAC AGGCTGCGAA GCAAGGACAC GTCAATCTAA CGATCAGACG AAAAACGATC 
TATCCGGTTC CAAAGGCAGA GAACGAAGCT CCTGCATCAC CAGCATCCTC GCACCATAGC 
AGCACCCAGC CGGCGTCTCT AACGGAGGAG AAGCGGACGC CCCAAGGCAG CCAAAACTCC 
TTAAACACTG TCAGTTCAGG CAGCGGCAGC ACCAGCGGCA TTGGCAGTGG CGGAGGCGGC 
GGGAGCGGCA ACGTCAGCAC CGTGGTTCAG CCGTATGATG TCGAGATCCG ACGCGGTGAA 
AATGAAGGAT TTGGATTTGT CATTGTGTCT TCTGTCAGCC GGCCAGAAGC CGGGACTACT 
TTTGCCGGCA ATGCATGTGT GGCCATGCCT CACAAAATAG GTCGGATAAT AGAGGGGAGC 
CCCGCGGATC GCTGCGGCAA GCTGAAAGTG GGGGACCGGA TCTTGGCCGT TAACGGGTGC 
TCCATCACCA ACAAGTCACA CTCGGACATC GTCAATTTAA TAAAGGAAGC GGGGAACACC 
GTGACTCTGC GCATCATTCC CGGCGATGAA ACGTCCAACG CGTCTTTATT AACCAATGCA 
GAGAAGATTG CCACGATTAC GACCACGCAT ACGCCACAAA CGATACCCCA AGAAACAAAA 
AATAATGCAA AACCAAAGCC GGAGCCCCAG TTTGAATTCA AGAACTCACC AGCCACACAG 
CCTGATCAAG AAATTTATTC AGTGGAACTA GACCGAGGAT TAAAAGGATT TGGGTTTAGT 
CTACGAGGAG GTCGAGAATA TAACATGGAT CTCTATGTTT TACGCCTTGC GGAGGATGGG 
CCTGCAGCCA GATGTGGGAA AATGAGGGTT GGCGATGAAA TCCTGGAAAT AAACGGAGAG 
ACCACAAAGA ACATGAAGCA CGCCCGGGCA ATAGAACTGA TTAAAAATGG TGGCCGAAGA 
GTACGCCTGT GTCTGAAACG CGGAGATGGT TCCGTACCGG AATATGAACC CGGCGTTGAC 
GGAAACAGCC CCACAACCAG TCCACAAAGT GTATCGAAAA TGAACTCGTT ACCATCTGAT 
AGAAATCACT CATCACTGGA GTTCAATTAT CCATCCGATC TTCACAAATC ATTACAGCAT 
GGAGACAAAC GAGCTTACGT TAAAGACCCT AAAGGCAACA GGGAGTATGG CAGACCACAT 
AACGAACATC ACACTTGGAA TGGATCTTCT AAAAATTATT CTGTACAAAA TAACAGAAAA 
GATCGATCAC CTGAGGGGAG GCGACCAAGA CAGGCCGATC GAACAATGAC GAACGGTCAG 
AAAAGACAGT CTCCCGAGAA AAGAAATGAA GGAACTAGGA GTGCTGAGAA CACACTGGAA 
AGGAGAGAGA AAAATGAGAG AAGAAAAGAG GTTTCTCCTC AAAAGCCCAG GGAAAGGTCG 
CCGGTGCGCA GAAGGGATGG TTCCCCAACC AGGCGAAGGA AATCCTTGGA TAGATTTATG 
GATCAAAGAC GATCCCCTGA TAGGAGAAGA GAGAGTTCAC CTGACCGAAG AAGAGCAAAG 
TCAACGGACA GGAGACGAGA AAGGTCACCA GAAAGGAGGA GAGAAAGGTC TCCTGAGAGA 
AGAAGAGAGA AGTCCACTGA GAGAAGAAGG GACAGGTCTC CTGATAAACG AAACAAAGAG 
CAACGAACCA GCAACAGAGA CAGGGATGAA CACAGTCTGA AATATGATAC CCTCAGAAGT 
GGCAGGCACC AACAAGAACA ACGGAGGAGG CAATACAAGG AATGCAGCAC CGACCTGAGC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_012961764.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817218.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa33043
Clone ID Related Accession (Same CDS sequence)	XM_012961764.2
Accession Version	XM_012961764.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683372.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG CAGGCGTAGT GCATACCGAC CACTATGAGT TTGATGATGA TGCTCCTGAA 
ATGAGCAGTA GCTTTACAGG AGACTCCAGT GAAGCAGACG AGCACACGAT TAACCTTTCC 
CAAGATAAAG TCCCACCGGC GGCTCGGATA CTCCCTACTT TTCCCATCAC GGAAGCTTCT 
CAGAATCTGC CTCATTACCT ACCTCCTGAC GAGGAAAGCC TTGGACCTCT ACCTGATAAC 
TGGGAAATGG CCTTTACAGA GAATGGAGAA GTCTATTTTA TAGACCATAA CACTAAAACG 
ACATCTTGGT TAGACCCCCG GTGCCTGAAC AAACAGCAGA AGCCTCTGGA AGAATGTGAA 
GATGATGAAT TGCCCGCTGG ATGGGAGAAA ATTGAAGACC CGGTTTATGG AGTCTACTAT 
GTAGATCACA TAAACAAGAA AACGCAGTAT GAAAATCCTG TATTGGAGGC CAAACGAAAG 
AAACAGCTTG GACAGCAGCA AAGTGAAGAA TGGACAGGGG ACCATCCAAC CATTGCTCCG 
CCCCCCATAA TGCTTCCTAA CAACCTTACT CCAAAAAAGA AGGAACCAGC AAGAGAAACG 
GCATCACATG TGAAACCGTT CTTCACCAGG AACCCCTCTG AACTGAAAGG CAAGTTCATC 
CATACGAAGC TAAGGAAAAG TAGTCGGGGC TTTGGCTTTA CCGTTGTCGG AGGGGACGAA 
CCAGATGAGT TCTTGCAAAT AAAAAGTTTG GTCGTCGACG GTCCGGCCGC GTTGGACGGC 
AAAATGGAAA CGGGTGATGT TATCGTTAGC GTGAACGATA TCTGCGTCCT TGGCTACACG 
CACGCTCAAG TTGTGAAGAT CTTCCAGTCT ATACCCATTG GGGAAAGTGT GGACCTTGAA 
CTATGCCGGG GTTATCCTCT TCCGTTTGAC CCAGATGACC CCAACACCAG CCTTGTGACA 
TCAGTTGCAA TATTGGACAA AGACCCTATC ATTGTCAATG GGCAAGAGAA TTATGACTCT 
CCGTCGAGCA ACAGCAGTAA AACTAGAAAA GCCAATAACG TCAAGGATCC AAGGCCTAGC 
AGCCCTGCTG ACATTTCTTC AAATGGTTCC CATGGTTACC CTAATGATAC AGTGTCCTTG 
GCATCCTCTA TAGCTACGCA ACCTGAACTA ATTACTGTGC ATATAGTAAA AGGACCAATG 
GGATTTGGCT TTACCATAGC AGACAGTCCT GGCGGTGGGG GCCAGAGAGT GAAGCAGATT 
GTAGATAGTC CAAGATGCCG GGGCCTCAAA GAAGGAGATC TTATAGTAGA GGTGAATAAA 
AAGAATGTTC AGAACCTTAC CCATAACCAA GTTGTGGATT TACTCATCGA ATGTCCTAAG 
GGAAGCGAAG TTACCCTACT GGTTCAAAGA GGAGGACTCC CGGTCCCAAA GAAGAGCCCA 
AAGTCGCAAC CACTTGAAAG GAAAGACAGC CAAAACAGCT CGCAGCACAG TGTTTCCAGC 
CAGCACAGTG GGCACACTGC TTCACCCCAT CACGCCGCGC CTGGGCTCCC CGATTATCCT 
ACCAGCGAGG CGCCGGCTGT CGACCAAGCC GACAACTCCG GGCAGAAAAA GCCTGATCCT 
TTCAAAATCT GGGCCCAATC CAGGAGCATG TATGAGAACA GACTTCCAGA CTTTCAAGAG 
CATGATATAT TTTTGTGGCG AAAGGAAACT GGATTTGGGT TTCGAATTCT CGGTGGAAAT 
GAGCCTGGAG AACCAATCTT CATAGGTCAT ATTGTGCCAC TGGGGTCAGC GGATGCCGAC 
GGGCGGTTGC GCTCTGGTGA CGAGCTTATC TGTGTGGATG GAACAGCGGT TGTTGGAAAA 
TCTCATCAAC TCGTCGTACA GCTTATGCAA CAGGCTGCGA AGCAAGGACA CGTCAATCTA 
ACGATCAGAC GAAAAACGAT CTATCCGGTT CCAAAGGCAG AGAACGAAGC TCCTGCATCA 
CCAGCATCCT CGCACCATAG CAGCACCCAG CCGGCGTCTC TAACGGAGGA GAAGCGGACG 
CCCCAAGGCA GCCAAAACTC CTTAAACACT GTCAGTTCAG GCAGCGGCAG CACCAGCGGC 
ATTGGCAGTG GCGGAGGCGG CGGGAGCGGC AACGTCAGCA CCGTGGTTCA GCCGTATGAT 
GTCGAGATCC GACGCGGTGA AAATGAAGGA TTTGGATTTG TCATTGTGTC TTCTGTCAGC 
CGGCCAGAAG CCGGGACTAC TTTTGCCGGC AATGCATGTG TGGCCATGCC TCACAAAATA 
GGTCGGATAA TAGAGGGGAG CCCCGCGGAT CGCTGCGGCA AGCTGAAAGT GGGGGACCGG 
ATCTTGGCCG TTAACGGGTG CTCCATCACC AACAAGTCAC ACTCGGACAT CGTCAATTTA 
ATAAAGGAAG CGGGGAACAC CGTGACTCTG CGCATCATTC CCGGCGATGA AACGTCCAAC 
GCGTCTTTAT TAACCAATGC AGAGAAGATT GCCACGATTA CGACCACGCA TACGCCACAA 
ACGATACCCC AAGAAACAAA AAATAATGCA AAACCAAAGC CGGAGCCCCA GTTTGAATTC 
AAGAACTCAC CAGCCACACA GCCTGATCAA GAAATTTATT CAGTGGAACT AGACCGAGGA 
TTAAAAGGAT TTGGGTTTAG TCTACGAGGA GGTCGAGAAT ATAACATGGA TCTCTATGTT 
TTACGCCTTG CGGAGGATGG GCCTGCAGCC AGATGTGGGA AAATGAGGGT TGGCGATGAA 
ATCCTGGAAA TAAACGGAGA GACCACAAAG AACATGAAGC ACGCCCGGGC AATAGAACTG 
ATTAAAAATG GTGGCCGAAG AGTACGCCTG TGTCTGAAAC GCGGAGATGG TTCCGTACCG 
GAATATGAAC CCGGCGTTGA CGGAAACAGC CCCACAACCA GTCCACAAAG TGTATCGAAA 
ATGAACTCGT TACCATCTGA TAGAAATCAC TCATCACTGG AGTTCAATTA TCCATCCGAT 
CTTCACAAAT CATTACAGCA TGGAGACAAA CGAGCTTACG TTAAAGACCC TAAAGGCAAC 
AGGGAGTATG GCAGACCACA TAACGAACAT CACACTTGGA ATGGATCTTC TAAAAATTAT 
TCTGTACAAA ATAACAGAAA AGATCGATCA CCTGAGGGGA GGCGACCAAG ACAGGCCGAT 
CGAACAATGA CGAACGGTCA GAAAAGACAG TCTCCCGAGA AAAGAAATGA AGGAACTAGG 
AGTGCTGAGA ACACACTGGA AAGGAGAGAG AAAAATGAGA GAAGAAAAGA GGTTTCTCCT 
CAAAAGCCCA GGGAAAGGTC GCCGGTGCGC AGAAGGGATG GTTCCCCAAC CAGGCGAAGG 
AAATCCTTGG ATAGATTTAT GGATCAAAGA CGATCCCCTG ATAGGAGAAG AGAGAGTTCA 
CCTGACCGAA GAAGAGCAAA GTCAACGGAC AGGAGACGAG AAAGGTCACC AGAAAGGAGG 
AGAGAAAGGT CTCCTGAGAG AAGAAGAGAG AAGTCCACTG AGAGAAGAAG GGACAGGTCT 
CCTGATAAAC GAAACAAAGA GCAACGAACC AGCAACAGAG ACAGGGATGA ACACAGTCTG 
AAATATGATA CCCTCAGAAG TGGCAGGCAC CAACAAGAAC AACGGAGGAG GCAATACAAG 
GAATGCAGCA CCGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817218.1
CDS	494..4180
Translation	MQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_012961764.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961764.2

ORF Insert Sequence:

ATGCAAAATG CAGGCGTAGT GCATACCGAC CACTATGAGT TTGATGATGA TGCTCCTGAA 
ATGAGCAGTA GCTTTACAGG AGACTCCAGT GAAGCAGACG AGCACACGAT TAACCTTTCC 
CAAGATAAAG TCCCACCGGC GGCTCGGATA CTCCCTACTT TTCCCATCAC GGAAGCTTCT 
CAGAATCTGC CTCATTACCT ACCTCCTGAC GAGGAAAGCC TTGGACCTCT ACCTGATAAC 
TGGGAAATGG CCTTTACAGA GAATGGAGAA GTCTATTTTA TAGACCATAA CACTAAAACG 
ACATCTTGGT TAGACCCCCG GTGCCTGAAC AAACAGCAGA AGCCTCTGGA AGAATGTGAA 
GATGATGAAT TGCCCGCTGG ATGGGAGAAA ATTGAAGACC CGGTTTATGG AGTCTACTAT 
GTAGATCACA TAAACAAGAA AACGCAGTAT GAAAATCCTG TATTGGAGGC CAAACGAAAG 
AAACAGCTTG GACAGCAGCA AAGTGAAGAA TGGACAGGGG ACCATCCAAC CATTGCTCCG 
CCCCCCATAA TGCTTCCTAA CAACCTTACT CCAAAAAAGA AGGAACCAGC AAGAGAAACG 
GCATCACATG TGAAACCGTT CTTCACCAGG AACCCCTCTG AACTGAAAGG CAAGTTCATC 
CATACGAAGC TAAGGAAAAG TAGTCGGGGC TTTGGCTTTA CCGTTGTCGG AGGGGACGAA 
CCAGATGAGT TCTTGCAAAT AAAAAGTTTG GTCGTCGACG GTCCGGCCGC GTTGGACGGC 
AAAATGGAAA CGGGTGATGT TATCGTTAGC GTGAACGATA TCTGCGTCCT TGGCTACACG 
CACGCTCAAG TTGTGAAGAT CTTCCAGTCT ATACCCATTG GGGAAAGTGT GGACCTTGAA 
CTATGCCGGG GTTATCCTCT TCCGTTTGAC CCAGATGACC CCAACACCAG CCTTGTGACA 
TCAGTTGCAA TATTGGACAA AGACCCTATC ATTGTCAATG GGCAAGAGAA TTATGACTCT 
CCGTCGAGCA ACAGCAGTAA AACTAGAAAA GCCAATAACG TCAAGGATCC AAGGCCTAGC 
AGCCCTGCTG ACATTTCTTC AAATGGTTCC CATGGTTACC CTAATGATAC AGTGTCCTTG 
GCATCCTCTA TAGCTACGCA ACCTGAACTA ATTACTGTGC ATATAGTAAA AGGACCAATG 
GGATTTGGCT TTACCATAGC AGACAGTCCT GGCGGTGGGG GCCAGAGAGT GAAGCAGATT 
GTAGATAGTC CAAGATGCCG GGGCCTCAAA GAAGGAGATC TTATAGTAGA GGTGAATAAA 
AAGAATGTTC AGAACCTTAC CCATAACCAA GTTGTGGATT TACTCATCGA ATGTCCTAAG 
GGAAGCGAAG TTACCCTACT GGTTCAAAGA GGAGGACTCC CGGTCCCAAA GAAGAGCCCA 
AAGTCGCAAC CACTTGAAAG GAAAGACAGC CAAAACAGCT CGCAGCACAG TGTTTCCAGC 
CAGCACAGTG GGCACACTGC TTCACCCCAT CACGCCGCGC CTGGGCTCCC CGATTATCCT 
ACCAGCGAGG CGCCGGCTGT CGACCAAGCC GACAACTCCG GGCAGAAAAA GCCTGATCCT 
TTCAAAATCT GGGCCCAATC CAGGAGCATG TATGAGAACA GACTTCCAGA CTTTCAAGAG 
CATGATATAT TTTTGTGGCG AAAGGAAACT GGATTTGGGT TTCGAATTCT CGGTGGAAAT 
GAGCCTGGAG AACCAATCTT CATAGGTCAT ATTGTGCCAC TGGGGTCAGC GGATGCCGAC 
GGGCGGTTGC GCTCTGGTGA CGAGCTTATC TGTGTGGATG GAACAGCGGT TGTTGGAAAA 
TCTCATCAAC TCGTCGTACA GCTTATGCAA CAGGCTGCGA AGCAAGGACA CGTCAATCTA 
ACGATCAGAC GAAAAACGAT CTATCCGGTT CCAAAGGCAG AGAACGAAGC TCCTGCATCA 
CCAGCATCCT CGCACCATAG CAGCACCCAG CCGGCGTCTC TAACGGAGGA GAAGCGGACG 
CCCCAAGGCA GCCAAAACTC CTTAAACACT GTCAGTTCAG GCAGCGGCAG CACCAGCGGC 
ATTGGCAGTG GCGGAGGCGG CGGGAGCGGC AACGTCAGCA CCGTGGTTCA GCCGTATGAT 
GTCGAGATCC GACGCGGTGA AAATGAAGGA TTTGGATTTG TCATTGTGTC TTCTGTCAGC 
CGGCCAGAAG CCGGGACTAC TTTTGCCGGC AATGCATGTG TGGCCATGCC TCACAAAATA 
GGTCGGATAA TAGAGGGGAG CCCCGCGGAT CGCTGCGGCA AGCTGAAAGT GGGGGACCGG 
ATCTTGGCCG TTAACGGGTG CTCCATCACC AACAAGTCAC ACTCGGACAT CGTCAATTTA 
ATAAAGGAAG CGGGGAACAC CGTGACTCTG CGCATCATTC CCGGCGATGA AACGTCCAAC 
GCGTCTTTAT TAACCAATGC AGAGAAGATT GCCACGATTA CGACCACGCA TACGCCACAA 
ACGATACCCC AAGAAACAAA AAATAATGCA AAACCAAAGC CGGAGCCCCA GTTTGAATTC 
AAGAACTCAC CAGCCACACA GCCTGATCAA GAAATTTATT CAGTGGAACT AGACCGAGGA 
TTAAAAGGAT TTGGGTTTAG TCTACGAGGA GGTCGAGAAT ATAACATGGA TCTCTATGTT 
TTACGCCTTG CGGAGGATGG GCCTGCAGCC AGATGTGGGA AAATGAGGGT TGGCGATGAA 
ATCCTGGAAA TAAACGGAGA GACCACAAAG AACATGAAGC ACGCCCGGGC AATAGAACTG 
ATTAAAAATG GTGGCCGAAG AGTACGCCTG TGTCTGAAAC GCGGAGATGG TTCCGTACCG 
GAATATGAAC CCGGCGTTGA CGGAAACAGC CCCACAACCA GTCCACAAAG TGTATCGAAA 
ATGAACTCGT TACCATCTGA TAGAAATCAC TCATCACTGG AGTTCAATTA TCCATCCGAT 
CTTCACAAAT CATTACAGCA TGGAGACAAA CGAGCTTACG TTAAAGACCC TAAAGGCAAC 
AGGGAGTATG GCAGACCACA TAACGAACAT CACACTTGGA ATGGATCTTC TAAAAATTAT 
TCTGTACAAA ATAACAGAAA AGATCGATCA CCTGAGGGGA GGCGACCAAG ACAGGCCGAT 
CGAACAATGA CGAACGGTCA GAAAAGACAG TCTCCCGAGA AAAGAAATGA AGGAACTAGG 
AGTGCTGAGA ACACACTGGA AAGGAGAGAG AAAAATGAGA GAAGAAAAGA GGTTTCTCCT 
CAAAAGCCCA GGGAAAGGTC GCCGGTGCGC AGAAGGGATG GTTCCCCAAC CAGGCGAAGG 
AAATCCTTGG ATAGATTTAT GGATCAAAGA CGATCCCCTG ATAGGAGAAG AGAGAGTTCA 
CCTGACCGAA GAAGAGCAAA GTCAACGGAC AGGAGACGAG AAAGGTCACC AGAAAGGAGG 
AGAGAAAGGT CTCCTGAGAG AAGAAGAGAG AAGTCCACTG AGAGAAGAAG GGACAGGTCT 
CCTGATAAAC GAAACAAAGA GCAACGAACC AGCAACAGAG ACAGGGATGA ACACAGTCTG 
AAATATGATA CCCTCAGAAG TGGCAGGCAC CAACAAGAAC AACGGAGGAG GCAATACAAG 
GAATGCAGCA CCGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_012961766.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817220.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X9, mRNA.

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CloneID	OXa33045
Clone ID Related Accession (Same CDS sequence)	XM_012961766.2
Accession Version	XM_012961766.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGGGGT ACACACCGAG GAACTGGACA GTGAACTAGA ATTGCCCGCT 
GGATGGGAGA AAATTGAAGA CCCGGTTTAT GGAGTCTACT ATGTAGATCA CATAAACAAG 
AAAACGCAGT ATGAAAATCC TGTATTGGAG GCCAAACGAA AGAAACAGCT TGGACAGCAG 
CAAAGTGAAG AATGGACAGG GGACCATCCA ACCATTGCTC CGCCCCCCAT AATGCTTCCT 
AACAACCTTA CTCCAAAAAA GAAGGAACCA GCAAGAGAAA CGGCATCACA TGTGAAACCG 
TTCTTCACCA GGAACCCCTC TGAACTGAAA GGCAAGTTCA TCCATACGAA GCTAAGGAAA 
AGTAGTCGGG GCTTTGGCTT TACCGTTGTC GGAGGGGACG AACCAGATGA GTTCTTGCAA 
ATAAAAAGTT TGGTCGTCGA CGGTCCGGCC GCGTTGGACG GCAAAATGGA AACGGGTGAT 
GTTATCGTTA GCGTGAACGA TATCTGCGTC CTTGGCTACA CGCACGCTCA AGTTGTGAAG 
ATCTTCCAGT CTATACCCAT TGGGGAAAGT GTGGACCTTG AACTATGCCG GGGTTATCCT 
CTTCCGTTTG ACCCAGATGA CCCCAACACC AGCCTTGTGA CATCAGTTGC AATATTGGAC 
AAAGACCCTA TCATTGTCAA TGGGCAAGAG AATTATGACT CTCCGTCGAG CAACAGCAGT 
AAAACTAGAA AAGCCAATAA CGTCAAGGAT CCAAGGCCTA GCAGCCCTGC TGACATTTCT 
TCAAATGGTT CCCATGGTTA CCCTAATGAT ACAGTGTCCT TGGCATCCTC TATAGCTACG 
CAACCTGAAC TAATTACTGT GCATATAGTA AAAGGACCAA TGGGATTTGG CTTTACCATA 
GCAGACAGTC CTGGCGGTGG GGGCCAGAGA GTGAAGCAGA TTGTAGATAG TCCAAGATGC 
CGGGGCCTCA AAGAAGGAGA TCTTATAGTA GAGGTGAATA AAAAGAATGT TCAGAACCTT 
ACCCATAACC AAGTTGTGGA TTTACTCATC GAATGTCCTA AGGGAAGCGA AGTTACCCTA 
CTGGTTCAAA GAGGAGGACT CCCGGTCCCA AAGAAGAGCC CAAAGTCGCA ACCACTTGAA 
AGGAAAGACA GCCAAAACAG CTCGCAGCAC AGTGTTTCCA GCCAGCACAG TGGGCACACT 
GCTTCACCCC ATCACGCCGC GCCTGGGCTC CCCGATTATC CTACCAGCGA GGCGCCGGCT 
GTCGACCAAG CCGACAACTC CGGGCAGAAA AAGCCTGATC CTTTCAAAAT CTGGGCCCAA 
TCCAGGAGCA TGTATGAGAA CAGACTTCCA GACTTTCAAG AGCATGATAT ATTTTTGTGG 
CGAAAGGAAA CTGGATTTGG GTTTCGAATT CTCGGTGGAA ATGAGCCTGG AGAACCAATC 
TTCATAGGTC ATATTGTGCC ACTGGGGTCA GCGGATGCCG ACGGGCGGTT GCGCTCTGGT 
GACGAGCTTA TCTGTGTGGA TGGAACAGCG GTTGTTGGAA AATCTCATCA ACTCGTCGTA 
CAGCTTATGC AACAGGCTGC GAAGCAAGGA CACGTCAATC TAACGATCAG ACGAAAAACG 
ATCTATCCGG TTCCAAAGGC AGAGAACGAA GCTCCTGCAT CACCAGCATC CTCGCACCAT 
AGCAGCACCC AGCCGGCGTC TCTAACGGAG GAGAAGCGGA CGCCCCAAGG CAGCCAAAAC 
TCCTTAAACA CTGTCAGTTC AGGCAGCGGC AGCACCAGCG GCATTGGCAG TGGCGGAGGC 
GGCGGGAGCG GCAACGTCAG CACCGTGGTT CAGCCGTATG ATGTCGAGAT CCGACGCGGT 
GAAAATGAAG GATTTGGATT TGTCATTGTG TCTTCTGTCA GCCGGCCAGA AGCCGGGACT 
ACTTTTGGTC GGATAATAGA GGGGAGCCCC GCGGATCGCT GCGGCAAGCT GAAAGTGGGG 
GACCGGATCT TGGCCGTTAA CGGGTGCTCC ATCACCAACA AGTCACACTC GGACATCGTC 
AATTTAATAA AGGAAGCGGG GAACACCGTG ACTCTGCGCA TCATTCCCGG CGATGAAACG 
TCCAACGCGT CTTTATTAAC CAATGCAGAG AAGATTGCCA CGATTACGAC CACGCATACG 
CCACAAACGA TACCCCAAGA AACAAAAAAT AATGCAAAAC CAAAGCCGGA GCCCCAGTTT 
GAATTCAAGA ACTCACCAGC CACACAGCCT GATCAAGAAA TTTATTCAGT GGAACTAGAC 
CGAGGATTAA AAGGATTTGG GTTTAGTCTA CGAGGAGGTC GAGAATATAA CATGGATCTC 
TATGTTTTAC GCCTTGCGGA GGATGGGCCT GCAGCCAGAT GTGGGAAAAT GAGGGTTGGC 
GATGAAATCC TGGAAATAAA CGGAGAGACC ACAAAGAACA TGAAGCACGC CCGGGCAATA 
GAACTGATTA AAAATGGTGG CCGAAGAGTA CGCCTGTGTC TGAAACGCGG AGATGGTTCC 
GTACCGGAAT ATGAACCCGG CGTTGACGGA AACAGCCCCA CAACCAGTCC ACAAAGTGTA 
TCGAAAATGA ACTCGTTACC ATCTGATAGA AATCACTCAT CACTGGAGTT CAATTATCCA 
TCCGATCTTC ACAAATCATT ACAGCATGGA GACAAACGAG CTTACGTTAA AGACCCTAAA 
GGCAACAGGG AGTATGGCAG ACCACATAAC GAACATCACA CTTGGAATGG ATCTTCTAAA 
AATTATTCTG TACAAAATAA CAGAAAAGAT CGATCACCTG AGGGGAGGCG ACCAAGACAG 
GCCGATCGAA CAATGACGAA CGGTCAGAAA AGACAGTCTC CCGAGAAAAG AAATGAAGGA 
ACTAGGAGTG CTGAGAACAC ACTGGAAAGG AGAGAGAAAA ATGAGAGAAG AAAAGAGGTT 
TCTCCTCAAA AGCCCAGGGA AAGGTCGCCG GTGCGCAGAA GGGATGGTTC CCCAACCAGG 
CGAAGGAAAT CCTTGGATAG ATTTATGGAT CAAAGACGAT CCCCTGATAG GAGAAGAGAG 
AGTTCACCTG ACCGAAGAAG AGCAAAGTCA ACGGACAGGA GACGAGAAAG GTCACCAGAA 
AGGAGGAGAG AAAGGTCTCC TGAGAGAAGA AGAGAGAAGT CCACTGAGAG AAGAAGGGAC 
AGGTCTCCTG ATAAACGAAA CAAAGAGCAA CGAACCAGCA ACAGAGACAG GGATGAACAC 
AGTCTGAAAT ATGATACCCT CAGAAGTGGC AGGCACCAAC AAGAACAACG GAGGAGGCAA 
TACAAGGAAT GCAGCACCGA CCTGAGCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817220.1
CDS	284..4636
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_012961766.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961766.2

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGGGGT ACACACCGAG GAACTGGACA GTGAACTAGA ATTGCCCGCT 
GGATGGGAGA AAATTGAAGA CCCGGTTTAT GGAGTCTACT ATGTAGATCA CATAAACAAG 
AAAACGCAGT ATGAAAATCC TGTATTGGAG GCCAAACGAA AGAAACAGCT TGGACAGCAG 
CAAAGTGAAG AATGGACAGG GGACCATCCA ACCATTGCTC CGCCCCCCAT AATGCTTCCT 
AACAACCTTA CTCCAAAAAA GAAGGAACCA GCAAGAGAAA CGGCATCACA TGTGAAACCG 
TTCTTCACCA GGAACCCCTC TGAACTGAAA GGCAAGTTCA TCCATACGAA GCTAAGGAAA 
AGTAGTCGGG GCTTTGGCTT TACCGTTGTC GGAGGGGACG AACCAGATGA GTTCTTGCAA 
ATAAAAAGTT TGGTCGTCGA CGGTCCGGCC GCGTTGGACG GCAAAATGGA AACGGGTGAT 
GTTATCGTTA GCGTGAACGA TATCTGCGTC CTTGGCTACA CGCACGCTCA AGTTGTGAAG 
ATCTTCCAGT CTATACCCAT TGGGGAAAGT GTGGACCTTG AACTATGCCG GGGTTATCCT 
CTTCCGTTTG ACCCAGATGA CCCCAACACC AGCCTTGTGA CATCAGTTGC AATATTGGAC 
AAAGACCCTA TCATTGTCAA TGGGCAAGAG AATTATGACT CTCCGTCGAG CAACAGCAGT 
AAAACTAGAA AAGCCAATAA CGTCAAGGAT CCAAGGCCTA GCAGCCCTGC TGACATTTCT 
TCAAATGGTT CCCATGGTTA CCCTAATGAT ACAGTGTCCT TGGCATCCTC TATAGCTACG 
CAACCTGAAC TAATTACTGT GCATATAGTA AAAGGACCAA TGGGATTTGG CTTTACCATA 
GCAGACAGTC CTGGCGGTGG GGGCCAGAGA GTGAAGCAGA TTGTAGATAG TCCAAGATGC 
CGGGGCCTCA AAGAAGGAGA TCTTATAGTA GAGGTGAATA AAAAGAATGT TCAGAACCTT 
ACCCATAACC AAGTTGTGGA TTTACTCATC GAATGTCCTA AGGGAAGCGA AGTTACCCTA 
CTGGTTCAAA GAGGAGGACT CCCGGTCCCA AAGAAGAGCC CAAAGTCGCA ACCACTTGAA 
AGGAAAGACA GCCAAAACAG CTCGCAGCAC AGTGTTTCCA GCCAGCACAG TGGGCACACT 
GCTTCACCCC ATCACGCCGC GCCTGGGCTC CCCGATTATC CTACCAGCGA GGCGCCGGCT 
GTCGACCAAG CCGACAACTC CGGGCAGAAA AAGCCTGATC CTTTCAAAAT CTGGGCCCAA 
TCCAGGAGCA TGTATGAGAA CAGACTTCCA GACTTTCAAG AGCATGATAT ATTTTTGTGG 
CGAAAGGAAA CTGGATTTGG GTTTCGAATT CTCGGTGGAA ATGAGCCTGG AGAACCAATC 
TTCATAGGTC ATATTGTGCC ACTGGGGTCA GCGGATGCCG ACGGGCGGTT GCGCTCTGGT 
GACGAGCTTA TCTGTGTGGA TGGAACAGCG GTTGTTGGAA AATCTCATCA ACTCGTCGTA 
CAGCTTATGC AACAGGCTGC GAAGCAAGGA CACGTCAATC TAACGATCAG ACGAAAAACG 
ATCTATCCGG TTCCAAAGGC AGAGAACGAA GCTCCTGCAT CACCAGCATC CTCGCACCAT 
AGCAGCACCC AGCCGGCGTC TCTAACGGAG GAGAAGCGGA CGCCCCAAGG CAGCCAAAAC 
TCCTTAAACA CTGTCAGTTC AGGCAGCGGC AGCACCAGCG GCATTGGCAG TGGCGGAGGC 
GGCGGGAGCG GCAACGTCAG CACCGTGGTT CAGCCGTATG ATGTCGAGAT CCGACGCGGT 
GAAAATGAAG GATTTGGATT TGTCATTGTG TCTTCTGTCA GCCGGCCAGA AGCCGGGACT 
ACTTTTGGTC GGATAATAGA GGGGAGCCCC GCGGATCGCT GCGGCAAGCT GAAAGTGGGG 
GACCGGATCT TGGCCGTTAA CGGGTGCTCC ATCACCAACA AGTCACACTC GGACATCGTC 
AATTTAATAA AGGAAGCGGG GAACACCGTG ACTCTGCGCA TCATTCCCGG CGATGAAACG 
TCCAACGCGT CTTTATTAAC CAATGCAGAG AAGATTGCCA CGATTACGAC CACGCATACG 
CCACAAACGA TACCCCAAGA AACAAAAAAT AATGCAAAAC CAAAGCCGGA GCCCCAGTTT 
GAATTCAAGA ACTCACCAGC CACACAGCCT GATCAAGAAA TTTATTCAGT GGAACTAGAC 
CGAGGATTAA AAGGATTTGG GTTTAGTCTA CGAGGAGGTC GAGAATATAA CATGGATCTC 
TATGTTTTAC GCCTTGCGGA GGATGGGCCT GCAGCCAGAT GTGGGAAAAT GAGGGTTGGC 
GATGAAATCC TGGAAATAAA CGGAGAGACC ACAAAGAACA TGAAGCACGC CCGGGCAATA 
GAACTGATTA AAAATGGTGG CCGAAGAGTA CGCCTGTGTC TGAAACGCGG AGATGGTTCC 
GTACCGGAAT ATGAACCCGG CGTTGACGGA AACAGCCCCA CAACCAGTCC ACAAAGTGTA 
TCGAAAATGA ACTCGTTACC ATCTGATAGA AATCACTCAT CACTGGAGTT CAATTATCCA 
TCCGATCTTC ACAAATCATT ACAGCATGGA GACAAACGAG CTTACGTTAA AGACCCTAAA 
GGCAACAGGG AGTATGGCAG ACCACATAAC GAACATCACA CTTGGAATGG ATCTTCTAAA 
AATTATTCTG TACAAAATAA CAGAAAAGAT CGATCACCTG AGGGGAGGCG ACCAAGACAG 
GCCGATCGAA CAATGACGAA CGGTCAGAAA AGACAGTCTC CCGAGAAAAG AAATGAAGGA 
ACTAGGAGTG CTGAGAACAC ACTGGAAAGG AGAGAGAAAA ATGAGAGAAG AAAAGAGGTT 
TCTCCTCAAA AGCCCAGGGA AAGGTCGCCG GTGCGCAGAA GGGATGGTTC CCCAACCAGG 
CGAAGGAAAT CCTTGGATAG ATTTATGGAT CAAAGACGAT CCCCTGATAG GAGAAGAGAG 
AGTTCACCTG ACCGAAGAAG AGCAAAGTCA ACGGACAGGA GACGAGAAAG GTCACCAGAA 
AGGAGGAGAG AAAGGTCTCC TGAGAGAAGA AGAGAGAAGT CCACTGAGAG AAGAAGGGAC 
AGGTCTCCTG ATAAACGAAA CAAAGAGCAA CGAACCAGCA ACAGAGACAG GGATGAACAC 
AGTCTGAAAT ATGATACCCT CAGAAGTGGC AGGCACCAAC AAGAACAACG GAGGAGGCAA 
TACAAGGAAT GCAGCACCGA CCTGAGCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901239.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757099.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OXa33046
Clone ID Related Accession (Same CDS sequence)	XM_031901239.1
Accession Version	XM_031901239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGGGGT ACACACCGAG GAACTGGACA GTGAACTAGA ATTGCCCGCT 
GGATGGGAGA AAATTGAAGA CCCGGTTTAT GGAGTCTACT ATGTAGATCA CATAAACAAG 
AAAACGCAGT ATGAAAATCC TGTATTGGAG GCCAAACGAA AGAAACAGCT TGGACAGCAG 
CAAAGTGAAG AATGGACAGG GGACCATCCA ACCATTGCTC CGCCCCCCAT AATGCTTCCT 
AACAACCTTA CTCCAAAAAA GAAGGAACCA GCAAGAGAAA CGGCATCACA TGTGAAACCG 
TTCTTCACCA GGAACCCCTC TGAACTGAAA GGCAAGTTCA TCCATACGAA GCTAAGGAAA 
AGTAGTCGGG GCTTTGGCTT TACCGTTGTC GGAGGGGACG AACCAGATGA GTTCTTGCAA 
ATAAAAAGTT TGGTCGTCGA CGGTCCGGCC GCGTTGGACG GCAAAATGGA AACGGGTGAT 
GTTATCGTTA GCGTGAACGA TATCTGCGTC CTTGGCTACA CGCACGCTCA AGTTGTGAAG 
ATCTTCCAGT CTATACCCAT TGGGGAAAGT GTGGACCTTG AACTATGCCG GGGTTATCCT 
CTTCCGTTTG ACCCAGATGA CCCCAACACC AGCCTTGTGA CATCAGTTGC AATATTGGAC 
AAAGACCCTA TCATTGTCAA TGGGCAAGAG AATTATGACT CTCCGTCGAG CAACAGCAGT 
AAAACTAGAA AAGCCAATAA CGTCAAGGAT CCAAGGCCTA GCAGCCCTGC TGACATTTCT 
TCAAATGGTT CCCATGGTTA CCCTAATGAT ACAGTGTCCT TGGCATCCTC TATAGCTACG 
CAACCTGAAC TAATTACTGT GCATATAGTA AAAGGACCAA TGGGATTTGG CTTTACCATA 
GCAGACAGTC CTGGCGGTGG GGGCCAGAGA GTGAAGCAGA TTGTAGATAG TCCAAGATGC 
CGGGGCCTCA AAGAAGGAGA TCTTATAGTA GAGGTGAATA AAAAGAATGT TCAGAACCTT 
ACCCATAACC AAGTTGTGGA TTTACTCATC GAATGTCCTA AGGGAAGCGA AGTTACCCTA 
CTGGTTCAAA GAGGAGGACT CCCGGTCCCA AAGAAGAGCC CAAAGTCGCA ACCACTTGAA 
AGGAAAGACA GCCAAAACAG CTCGCAGCAC AGTGTTTCCA GCCAGCACAG TGGGCACACT 
GCTTCACCCC ATCACGCCGC GCCTGGGCTC CCCGATTATC CTACCAGCGA GGCGCCGGCT 
GTCGACCAAG CCGACAACTC CGGGCAGAAA AAGCCTGATC CTTTCAAAAT CTGGGCCCAA 
TCCAGGAGCA TGTATGAGAA CAGACTTCCA GACTTTCAAG AGCATGATAT ATTTTTGTGG 
CGAAAGGAAA CTGGATTTGG GTTTCGAATT CTCGGTGGAA ATGAGCCTGG AGAACCAATC 
TTCATAGGTC ATATTGTGCC ACTGGGGTCA GCGGATGCCG ACGGGCGGTT GCGCTCTGGT 
GACGAGCTTA TCTGTGTGGA TGGAACAGCG GTTGTTGGAA AATCTCATCA ACTCGTCGTA 
CAGCTTATGC AACAGGCTGC GAAGCAAGGA CACGTCAATC TAACGATCAG ACGAAAAACG 
ATCTATCCGG TTCCAAAGGC AGAGAACGAA GCTCCTGCAT CACCAGCATC CTCGCACCAT 
AGCAGCACCC AGCCGGCGTC TCTAACGGAG GAGAAGCGGA CGCCCCAAGG CAGCCAAAAC 
TCCTTAAACA CTGTCAGTTC AGGCAGCGGC AGCACCAGCG GCATTGGCAG TGGCGGAGGC 
GGCGGGAGCG GCAACGTCAG CACCGTGGTT CAGCCGTATG ATGTCGAGAT CCGACGCGGT 
GAAAATGAAG GATTTGGATT TGTCATTGTG TCTTCTGTCA GCCGGCCAGA AGCCGGGACT 
ACTTTTGCCG GCAATGCATG TGTGGCCATG CCTCACAAAA TAGGTCGGAT AATAGAGGGG 
AGCCCCGCGG ATCGCTGCGG CAAGCTGAAA GTGGGGGACC GGATCTTGGC CGTTAACGGG 
TGCTCCATCA CCAACAAGTC ACACTCGGAC ATCGTCAATT TAATAAAGGA AGCGGGGAAC 
ACCGTGACTC TGCGCATCAT TCCCGGCGAT GAAACGTCCA ACGCGTCTTT ATTAACCAAT 
GCAGAGAAGA TTGCCACGAT TACGACCACG CATACGCCAC AAACGATACC CCAAGAAACA 
AAAAATAATG CAAAACCAAA GCCGGAGCCC CAGTTTGAAT TCAAGAACTC ACCAGCCACA 
CAGCCTGATC AAGAAATTTA TTCAGTGGAA CTAGACCGAG GATTAAAAGG ATTTGGGTTT 
AGTCTACGAG GAGGTCGAGA ATATAACATG GATCTCTATG TTTTACGCCT TGCGGAGGAT 
GGGCCTGCAG CCAGATGTGG GAAAATGAGG GTTGGCGATG AAATCCTGGA AATAAACGGA 
GAGACCACAA AGAACATGAA GCACGCCCGG GCAATAGAAC TGATTAAAAA TGGTGGCCGA 
AGAGTACGCC TGTGTCTGAA ACGCGGAGAT GGTTCCGTAC CGGAATATGC GATGATTCCT 
CCTAATATCG CTGCATGTAT GAGAAATGAC AAGCTCGGGG AACCTTGCTT CTACCTAATG 
GGCCAAAATC AAACTACGAA CCCGGCGTTG ACGGAAACAG CCCCACAACC AGTCCACAAA 
GTGTATCGAA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757099.1
CDS	278..4012
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYAMIPPNIAACMRNDKLGEPCFYLMGQNQTTNPALTETAPQPVHKVYRK

Target ORF information:

RefSeq Version	XM_031901239.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901239.1

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGGGGT ACACACCGAG GAACTGGACA GTGAACTAGA ATTGCCCGCT 
GGATGGGAGA AAATTGAAGA CCCGGTTTAT GGAGTCTACT ATGTAGATCA CATAAACAAG 
AAAACGCAGT ATGAAAATCC TGTATTGGAG GCCAAACGAA AGAAACAGCT TGGACAGCAG 
CAAAGTGAAG AATGGACAGG GGACCATCCA ACCATTGCTC CGCCCCCCAT AATGCTTCCT 
AACAACCTTA CTCCAAAAAA GAAGGAACCA GCAAGAGAAA CGGCATCACA TGTGAAACCG 
TTCTTCACCA GGAACCCCTC TGAACTGAAA GGCAAGTTCA TCCATACGAA GCTAAGGAAA 
AGTAGTCGGG GCTTTGGCTT TACCGTTGTC GGAGGGGACG AACCAGATGA GTTCTTGCAA 
ATAAAAAGTT TGGTCGTCGA CGGTCCGGCC GCGTTGGACG GCAAAATGGA AACGGGTGAT 
GTTATCGTTA GCGTGAACGA TATCTGCGTC CTTGGCTACA CGCACGCTCA AGTTGTGAAG 
ATCTTCCAGT CTATACCCAT TGGGGAAAGT GTGGACCTTG AACTATGCCG GGGTTATCCT 
CTTCCGTTTG ACCCAGATGA CCCCAACACC AGCCTTGTGA CATCAGTTGC AATATTGGAC 
AAAGACCCTA TCATTGTCAA TGGGCAAGAG AATTATGACT CTCCGTCGAG CAACAGCAGT 
AAAACTAGAA AAGCCAATAA CGTCAAGGAT CCAAGGCCTA GCAGCCCTGC TGACATTTCT 
TCAAATGGTT CCCATGGTTA CCCTAATGAT ACAGTGTCCT TGGCATCCTC TATAGCTACG 
CAACCTGAAC TAATTACTGT GCATATAGTA AAAGGACCAA TGGGATTTGG CTTTACCATA 
GCAGACAGTC CTGGCGGTGG GGGCCAGAGA GTGAAGCAGA TTGTAGATAG TCCAAGATGC 
CGGGGCCTCA AAGAAGGAGA TCTTATAGTA GAGGTGAATA AAAAGAATGT TCAGAACCTT 
ACCCATAACC AAGTTGTGGA TTTACTCATC GAATGTCCTA AGGGAAGCGA AGTTACCCTA 
CTGGTTCAAA GAGGAGGACT CCCGGTCCCA AAGAAGAGCC CAAAGTCGCA ACCACTTGAA 
AGGAAAGACA GCCAAAACAG CTCGCAGCAC AGTGTTTCCA GCCAGCACAG TGGGCACACT 
GCTTCACCCC ATCACGCCGC GCCTGGGCTC CCCGATTATC CTACCAGCGA GGCGCCGGCT 
GTCGACCAAG CCGACAACTC CGGGCAGAAA AAGCCTGATC CTTTCAAAAT CTGGGCCCAA 
TCCAGGAGCA TGTATGAGAA CAGACTTCCA GACTTTCAAG AGCATGATAT ATTTTTGTGG 
CGAAAGGAAA CTGGATTTGG GTTTCGAATT CTCGGTGGAA ATGAGCCTGG AGAACCAATC 
TTCATAGGTC ATATTGTGCC ACTGGGGTCA GCGGATGCCG ACGGGCGGTT GCGCTCTGGT 
GACGAGCTTA TCTGTGTGGA TGGAACAGCG GTTGTTGGAA AATCTCATCA ACTCGTCGTA 
CAGCTTATGC AACAGGCTGC GAAGCAAGGA CACGTCAATC TAACGATCAG ACGAAAAACG 
ATCTATCCGG TTCCAAAGGC AGAGAACGAA GCTCCTGCAT CACCAGCATC CTCGCACCAT 
AGCAGCACCC AGCCGGCGTC TCTAACGGAG GAGAAGCGGA CGCCCCAAGG CAGCCAAAAC 
TCCTTAAACA CTGTCAGTTC AGGCAGCGGC AGCACCAGCG GCATTGGCAG TGGCGGAGGC 
GGCGGGAGCG GCAACGTCAG CACCGTGGTT CAGCCGTATG ATGTCGAGAT CCGACGCGGT 
GAAAATGAAG GATTTGGATT TGTCATTGTG TCTTCTGTCA GCCGGCCAGA AGCCGGGACT 
ACTTTTGCCG GCAATGCATG TGTGGCCATG CCTCACAAAA TAGGTCGGAT AATAGAGGGG 
AGCCCCGCGG ATCGCTGCGG CAAGCTGAAA GTGGGGGACC GGATCTTGGC CGTTAACGGG 
TGCTCCATCA CCAACAAGTC ACACTCGGAC ATCGTCAATT TAATAAAGGA AGCGGGGAAC 
ACCGTGACTC TGCGCATCAT TCCCGGCGAT GAAACGTCCA ACGCGTCTTT ATTAACCAAT 
GCAGAGAAGA TTGCCACGAT TACGACCACG CATACGCCAC AAACGATACC CCAAGAAACA 
AAAAATAATG CAAAACCAAA GCCGGAGCCC CAGTTTGAAT TCAAGAACTC ACCAGCCACA 
CAGCCTGATC AAGAAATTTA TTCAGTGGAA CTAGACCGAG GATTAAAAGG ATTTGGGTTT 
AGTCTACGAG GAGGTCGAGA ATATAACATG GATCTCTATG TTTTACGCCT TGCGGAGGAT 
GGGCCTGCAG CCAGATGTGG GAAAATGAGG GTTGGCGATG AAATCCTGGA AATAAACGGA 
GAGACCACAA AGAACATGAA GCACGCCCGG GCAATAGAAC TGATTAAAAA TGGTGGCCGA 
AGAGTACGCC TGTGTCTGAA ACGCGGAGAT GGTTCCGTAC CGGAATATGC GATGATTCCT 
CCTAATATCG CTGCATGTAT GAGAAATGAC AAGCTCGGGG AACCTTGCTT CTACCTAATG 
GGCCAAAATC AAACTACGAA CCCGGCGTTG ACGGAAACAG CCCCACAACC AGTCCACAAA 
GTGTATCGAA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901227.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757087.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa57624
Clone ID Related Accession (Same CDS sequence)	XM_031901227.1
Accession Version	XM_031901227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGGCATCGGC 
TCTCCAGAAT TGCCCGCTGG ATGGGAGAAA ATTGAAGACC CGGTTTATGG AGTCTACTAT 
GTAGATCACA TAAACAAGAA AACGCAGTAT GAAAATCCTG TATTGGAGGC CAAACGAAAG 
AAACAGCTTG GACAGCAGCA AAGTGAAGAA TGGACAGGGG ACCATCCAAC CATTGCTCCG 
CCCCCCATAA TGCTTCCTAA CAACCTTACT CCAAAAAAGA AGGAACCAGC AAGAGAAACG 
GCATCACATG TGAAACCGTT CTTCACCAGG AACCCCTCTG AACTGAAAGG CAAGTTCATC 
CATACGAAGC TAAGGAAAAG TAGTCGGGGC TTTGGCTTTA CCGTTGTCGG AGGGGACGAA 
CCAGATGAGT TCTTGCAAAT AAAAAGTTTG GTCGTCGACG GTCCGGCCGC GTTGGACGGC 
AAAATGGAAA CGGGTGATGT TATCGTTAGC GTGAACGATA TCTGCGTCCT TGGCTACACG 
CACGCTCAAG TTGTGAAGAT CTTCCAGTCT ATACCCATTG GGGAAAGTGT GGACCTTGAA 
CTATGCCGGG GTTATCCTCT TCCGTTTGAC CCAGATGACC CCAACACCAG CCTTGTGACA 
TCAGTTGCAA TATTGGACAA AGACCCTATC ATTGTCAATG GGCAAGAGAA TTATGACTCT 
CCGTCGAGCA ACAGCAGTAA AACTAGAAAA GCCAATAACG TCAAGGATCC AAGGCCTAGC 
AGCCCTGCTG ACATTTCTTC AAATGGTTCC CATGGTTACC CTAATGATAC AGTGTCCTTG 
GCATCCTCTA TAGCTACGCA ACCTGAACTA ATTACTGTGC ATATAGTAAA AGGACCAATG 
GGATTTGGCT TTACCATAGC AGACAGTCCT GGCGGTGGGG GCCAGAGAGT GAAGCAGATT 
GTAGATAGTC CAAGATGCCG GGGCCTCAAA GAAGGAGATC TTATAGTAGA GGTGAATAAA 
AAGAATGTTC AGAACCTTAC CCATAACCAA GTTGTGGATT TACTCATCGA ATGTCCTAAG 
GGAAGCGAAG TTACCCTACT GGTTCAAAGA GGAGGACTCC CGGTCCCAAA GAAGAGCCCA 
AAGTCGCAAC CACTTGAAAG GAAAGACAGC CAAAACAGCT CGCAGCACAG TGTTTCCAGC 
CAGCACAGTG GGCACACTGC TTCACCCCAT CACGCCGCGC CTGGGCTCCC CGATTATCCT 
ACCAGCGAGG CGCCGGCTGT CGACCAAGCC GACAACTCCG GGCAGAAAAA GCCTGATCCT 
TTCAAAATCT GGGCCCAATC CAGGAGCATG TATGAGAACA GACTTCCAGA CTTTCAAGAG 
CATGATATAT TTTTGTGGCG AAAGGAAACT GGATTTGGGT TTCGAATTCT CGGTGGAAAT 
GAGCCTGGAG AACCAATCTT CATAGGTCAT ATTGTGCCAC TGGGGTCAGC GGATGCCGAC 
GGGCGGTTGC GCTCTGGTGA CGAGCTTATC TGTGTGGATG GAACAGCGGT TGTTGGAAAA 
TCTCATCAAC TCGTCGTACA GCTTATGCAA CAGGCTGCGA AGCAAGGACA CGTCAATCTA 
ACGATCAGAC GAAAAACGAT CTATCCGGTT CCAAAGGCAG AGAACGAAGC TCCTGCATCA 
CCAGCATCCT CGCACCATAG CAGCACCCAG CCGGCGTCTC TAACGGAGGA GAAGCGGACG 
CCCCAAGGCA GCCAAAACTC CTTAAACACT GTCAGTTCAG GCAGCGGCAG CACCAGCGGC 
ATTGGCAGTG GCGGAGGCGG CGGGAGCGGC AACGTCAGCA CCGTGGTTCA GCCGTATGAT 
GTCGAGATCC GACGCGGTGA AAATGAAGGA TTTGGATTTG TCATTGTGTC TTCTGTCAGC 
CGGCCAGAAG CCGGGACTAC TTTTGCCGGC AATGCATGTG TGGCCATGCC TCACAAAATA 
GGTCGGATAA TAGAGGGGAG CCCCGCGGAT CGCTGCGGCA AGCTGAAAGT GGGGGACCGG 
ATCTTGGCCG TTAACGGGTG CTCCATCACC AACAAGTCAC ACTCGGACAT CGTCAATTTA 
ATAAAGGAAG CGGGGAACAC CGTGACTCTG CGCATCATTC CCGGCGATGA AACGTCCAAC 
GCGTCTTTAT TAACCAATGC AGAGAAGATT GCCACGATTA CGACCACGCA TACGCCACAA 
ACGATACCCC AAGAAACAAA AAATAATGCA AAACCAAAGC CGGAGCCCCA GTTTGAATTC 
AAGAACTCAC CAGCCACACA GCCTGATCAA GAAATTTATT CAGTGGAACT AGACCGAGGA 
TTAAAAGGAT TTGGGTTTAG TCTACGAGGA GGTCGAGAAT ATAACATGGA TCTCTATGTT 
TTACGCCTTG CGGAGGATGG GCCTGCAGCC AGATGTGGGA AAATGAGGGT TGGCGATGAA 
ATCCTGGAAA TAAACGGAGA GACCACAAAG AACATGAAGC ACGCCCGGGC AATAGAACTG 
ATTAAAAATG GTGGCCGAAG AGTACGCCTG TGTCTGAAAC GCGGAGATGG TTCCGTACCG 
GAATATGAAC CCGGCGTTGA CGGAAACAGC CCCACAACCA GTCCACAAAG TGTATCGAAA 
ATGAACTCGT TACCATCTGA TAGAAATCAC TCATCACTGG AGTTCAATTA TCCATCCGAT 
CTTCACAAAT CATTACAGCA TGGAGACAAA CGAGCTTACG TTAAAGACCC TAAAGGCAAC 
AGGGAGTATG GCAGACCACA TAACGAACAT CACACTTGGA ATGGATCTTC TAAAAATTAT 
TCTGTACAAA ATAACAGAAA AGATCGATCA CCTGAGGGGA GGCGACCAAG ACAGGCCGAT 
CGAACAATGA CGAACGGTCA GAAAAGACAG TCTCCTGAGA AAAGAAATGA AGGAACTAGG 
AGTGCTGAGA ACACACTGGA AAGGAGAGAG AAAAATGAGA GAAGAAAAGA GGTTTCTCCT 
CAAAAGCCCA GGGAAAGGTC GCCGGTGCGC AGAAGGGATG GTTCCCCAAC CAGGCGAAGG 
AAATCCTTGG ATAGATTTAT GGATCAAAGA CGATCCCCTG ATAGGAGAAG AGAGAGTTCA 
CCTGACCGAA GAAGAGCAAA GTCAACGGAC AGGAGACGAG AAAGGTCACC AGAAAGGAGG 
AGAGAAAGGT CTCCTGAGAG AAGAAGAGAG AAGTCCACTG AGAGAAGAAG GGACAGGTCT 
CCTGATAAAC GAAACAAAGA GCAACGAACC AGCAACAGAG ACAGGGATGA ACACAGTCTG 
AAATATGATA CCCTCAGAAG TGGCAGGCAC CAACAAGAAC AACGGAGGAG GCAATACAAG 
GAATGCAGCA CCGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757087.1
CDS	277..4683
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEEGVHTEELDSELGIGSPELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_031901227.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901227.1

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGGCATCGGC 
TCTCCAGAAT TGCCCGCTGG ATGGGAGAAA ATTGAAGACC CGGTTTATGG AGTCTACTAT 
GTAGATCACA TAAACAAGAA AACGCAGTAT GAAAATCCTG TATTGGAGGC CAAACGAAAG 
AAACAGCTTG GACAGCAGCA AAGTGAAGAA TGGACAGGGG ACCATCCAAC CATTGCTCCG 
CCCCCCATAA TGCTTCCTAA CAACCTTACT CCAAAAAAGA AGGAACCAGC AAGAGAAACG 
GCATCACATG TGAAACCGTT CTTCACCAGG AACCCCTCTG AACTGAAAGG CAAGTTCATC 
CATACGAAGC TAAGGAAAAG TAGTCGGGGC TTTGGCTTTA CCGTTGTCGG AGGGGACGAA 
CCAGATGAGT TCTTGCAAAT AAAAAGTTTG GTCGTCGACG GTCCGGCCGC GTTGGACGGC 
AAAATGGAAA CGGGTGATGT TATCGTTAGC GTGAACGATA TCTGCGTCCT TGGCTACACG 
CACGCTCAAG TTGTGAAGAT CTTCCAGTCT ATACCCATTG GGGAAAGTGT GGACCTTGAA 
CTATGCCGGG GTTATCCTCT TCCGTTTGAC CCAGATGACC CCAACACCAG CCTTGTGACA 
TCAGTTGCAA TATTGGACAA AGACCCTATC ATTGTCAATG GGCAAGAGAA TTATGACTCT 
CCGTCGAGCA ACAGCAGTAA AACTAGAAAA GCCAATAACG TCAAGGATCC AAGGCCTAGC 
AGCCCTGCTG ACATTTCTTC AAATGGTTCC CATGGTTACC CTAATGATAC AGTGTCCTTG 
GCATCCTCTA TAGCTACGCA ACCTGAACTA ATTACTGTGC ATATAGTAAA AGGACCAATG 
GGATTTGGCT TTACCATAGC AGACAGTCCT GGCGGTGGGG GCCAGAGAGT GAAGCAGATT 
GTAGATAGTC CAAGATGCCG GGGCCTCAAA GAAGGAGATC TTATAGTAGA GGTGAATAAA 
AAGAATGTTC AGAACCTTAC CCATAACCAA GTTGTGGATT TACTCATCGA ATGTCCTAAG 
GGAAGCGAAG TTACCCTACT GGTTCAAAGA GGAGGACTCC CGGTCCCAAA GAAGAGCCCA 
AAGTCGCAAC CACTTGAAAG GAAAGACAGC CAAAACAGCT CGCAGCACAG TGTTTCCAGC 
CAGCACAGTG GGCACACTGC TTCACCCCAT CACGCCGCGC CTGGGCTCCC CGATTATCCT 
ACCAGCGAGG CGCCGGCTGT CGACCAAGCC GACAACTCCG GGCAGAAAAA GCCTGATCCT 
TTCAAAATCT GGGCCCAATC CAGGAGCATG TATGAGAACA GACTTCCAGA CTTTCAAGAG 
CATGATATAT TTTTGTGGCG AAAGGAAACT GGATTTGGGT TTCGAATTCT CGGTGGAAAT 
GAGCCTGGAG AACCAATCTT CATAGGTCAT ATTGTGCCAC TGGGGTCAGC GGATGCCGAC 
GGGCGGTTGC GCTCTGGTGA CGAGCTTATC TGTGTGGATG GAACAGCGGT TGTTGGAAAA 
TCTCATCAAC TCGTCGTACA GCTTATGCAA CAGGCTGCGA AGCAAGGACA CGTCAATCTA 
ACGATCAGAC GAAAAACGAT CTATCCGGTT CCAAAGGCAG AGAACGAAGC TCCTGCATCA 
CCAGCATCCT CGCACCATAG CAGCACCCAG CCGGCGTCTC TAACGGAGGA GAAGCGGACG 
CCCCAAGGCA GCCAAAACTC CTTAAACACT GTCAGTTCAG GCAGCGGCAG CACCAGCGGC 
ATTGGCAGTG GCGGAGGCGG CGGGAGCGGC AACGTCAGCA CCGTGGTTCA GCCGTATGAT 
GTCGAGATCC GACGCGGTGA AAATGAAGGA TTTGGATTTG TCATTGTGTC TTCTGTCAGC 
CGGCCAGAAG CCGGGACTAC TTTTGCCGGC AATGCATGTG TGGCCATGCC TCACAAAATA 
GGTCGGATAA TAGAGGGGAG CCCCGCGGAT CGCTGCGGCA AGCTGAAAGT GGGGGACCGG 
ATCTTGGCCG TTAACGGGTG CTCCATCACC AACAAGTCAC ACTCGGACAT CGTCAATTTA 
ATAAAGGAAG CGGGGAACAC CGTGACTCTG CGCATCATTC CCGGCGATGA AACGTCCAAC 
GCGTCTTTAT TAACCAATGC AGAGAAGATT GCCACGATTA CGACCACGCA TACGCCACAA 
ACGATACCCC AAGAAACAAA AAATAATGCA AAACCAAAGC CGGAGCCCCA GTTTGAATTC 
AAGAACTCAC CAGCCACACA GCCTGATCAA GAAATTTATT CAGTGGAACT AGACCGAGGA 
TTAAAAGGAT TTGGGTTTAG TCTACGAGGA GGTCGAGAAT ATAACATGGA TCTCTATGTT 
TTACGCCTTG CGGAGGATGG GCCTGCAGCC AGATGTGGGA AAATGAGGGT TGGCGATGAA 
ATCCTGGAAA TAAACGGAGA GACCACAAAG AACATGAAGC ACGCCCGGGC AATAGAACTG 
ATTAAAAATG GTGGCCGAAG AGTACGCCTG TGTCTGAAAC GCGGAGATGG TTCCGTACCG 
GAATATGAAC CCGGCGTTGA CGGAAACAGC CCCACAACCA GTCCACAAAG TGTATCGAAA 
ATGAACTCGT TACCATCTGA TAGAAATCAC TCATCACTGG AGTTCAATTA TCCATCCGAT 
CTTCACAAAT CATTACAGCA TGGAGACAAA CGAGCTTACG TTAAAGACCC TAAAGGCAAC 
AGGGAGTATG GCAGACCACA TAACGAACAT CACACTTGGA ATGGATCTTC TAAAAATTAT 
TCTGTACAAA ATAACAGAAA AGATCGATCA CCTGAGGGGA GGCGACCAAG ACAGGCCGAT 
CGAACAATGA CGAACGGTCA GAAAAGACAG TCTCCTGAGA AAAGAAATGA AGGAACTAGG 
AGTGCTGAGA ACACACTGGA AAGGAGAGAG AAAAATGAGA GAAGAAAAGA GGTTTCTCCT 
CAAAAGCCCA GGGAAAGGTC GCCGGTGCGC AGAAGGGATG GTTCCCCAAC CAGGCGAAGG 
AAATCCTTGG ATAGATTTAT GGATCAAAGA CGATCCCCTG ATAGGAGAAG AGAGAGTTCA 
CCTGACCGAA GAAGAGCAAA GTCAACGGAC AGGAGACGAG AAAGGTCACC AGAAAGGAGG 
AGAGAAAGGT CTCCTGAGAG AAGAAGAGAG AAGTCCACTG AGAGAAGAAG GGACAGGTCT 
CCTGATAAAC GAAACAAAGA GCAACGAACC AGCAACAGAG ACAGGGATGA ACACAGTCTG 
AAATATGATA CCCTCAGAAG TGGCAGGCAC CAACAAGAAC AACGGAGGAG GCAATACAAG 
GAATGCAGCA CCGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901228.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757088.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X2, mRNA.

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CloneID	OXa57625
Clone ID Related Accession (Same CDS sequence)	XM_031901228.1
Accession Version	XM_031901228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGGGGT ACACACCGAG GAACTGGACA GTGAACTAGG CATCGGCTCT 
CCAGAATTGC CCGCTGGATG GGAGAAAATT GAAGACCCGG TTTATGGAGT CTACTATGTA 
GATCACATAA ACAAGAAAAC GCAGTATGAA AATCCTGTAT TGGAGGCCAA ACGAAAGAAA 
CAGCTTGGAC AGCAGCAAAG TGAAGAATGG ACAGGGGACC ATCCAACCAT TGCTCCGCCC 
CCCATAATGC TTCCTAACAA CCTTACTCCA AAAAAGAAGG AACCAGCAAG AGAAACGGCA 
TCACATGTGA AACCGTTCTT CACCAGGAAC CCCTCTGAAC TGAAAGGCAA GTTCATCCAT 
ACGAAGCTAA GGAAAAGTAG TCGGGGCTTT GGCTTTACCG TTGTCGGAGG GGACGAACCA 
GATGAGTTCT TGCAAATAAA AAGTTTGGTC GTCGACGGTC CGGCCGCGTT GGACGGCAAA 
ATGGAAACGG GTGATGTTAT CGTTAGCGTG AACGATATCT GCGTCCTTGG CTACACGCAC 
GCTCAAGTTG TGAAGATCTT CCAGTCTATA CCCATTGGGG AAAGTGTGGA CCTTGAACTA 
TGCCGGGGTT ATCCTCTTCC GTTTGACCCA GATGACCCCA ACACCAGCCT TGTGACATCA 
GTTGCAATAT TGGACAAAGA CCCTATCATT GTCAATGGGC AAGAGAATTA TGACTCTCCG 
TCGAGCAACA GCAGTAAAAC TAGAAAAGCC AATAACGTCA AGGATCCAAG GCCTAGCAGC 
CCTGCTGACA TTTCTTCAAA TGGTTCCCAT GGTTACCCTA ATGATACAGT GTCCTTGGCA 
TCCTCTATAG CTACGCAACC TGAACTAATT ACTGTGCATA TAGTAAAAGG ACCAATGGGA 
TTTGGCTTTA CCATAGCAGA CAGTCCTGGC GGTGGGGGCC AGAGAGTGAA GCAGATTGTA 
GATAGTCCAA GATGCCGGGG CCTCAAAGAA GGAGATCTTA TAGTAGAGGT GAATAAAAAG 
AATGTTCAGA ACCTTACCCA TAACCAAGTT GTGGATTTAC TCATCGAATG TCCTAAGGGA 
AGCGAAGTTA CCCTACTGGT TCAAAGAGGA GGACTCCCGG TCCCAAAGAA GAGCCCAAAG 
TCGCAACCAC TTGAAAGGAA AGACAGCCAA AACAGCTCGC AGCACAGTGT TTCCAGCCAG 
CACAGTGGGC ACACTGCTTC ACCCCATCAC GCCGCGCCTG GGCTCCCCGA TTATCCTACC 
AGCGAGGCGC CGGCTGTCGA CCAAGCCGAC AACTCCGGGC AGAAAAAGCC TGATCCTTTC 
AAAATCTGGG CCCAATCCAG GAGCATGTAT GAGAACAGAC TTCCAGACTT TCAAGAGCAT 
GATATATTTT TGTGGCGAAA GGAAACTGGA TTTGGGTTTC GAATTCTCGG TGGAAATGAG 
CCTGGAGAAC CAATCTTCAT AGGTCATATT GTGCCACTGG GGTCAGCGGA TGCCGACGGG 
CGGTTGCGCT CTGGTGACGA GCTTATCTGT GTGGATGGAA CAGCGGTTGT TGGAAAATCT 
CATCAACTCG TCGTACAGCT TATGCAACAG GCTGCGAAGC AAGGACACGT CAATCTAACG 
ATCAGACGAA AAACGATCTA TCCGGTTCCA AAGGCAGAGA ACGAAGCTCC TGCATCACCA 
GCATCCTCGC ACCATAGCAG CACCCAGCCG GCGTCTCTAA CGGAGGAGAA GCGGACGCCC 
CAAGGCAGCC AAAACTCCTT AAACACTGTC AGTTCAGGCA GCGGCAGCAC CAGCGGCATT 
GGCAGTGGCG GAGGCGGCGG GAGCGGCAAC GTCAGCACCG TGGTTCAGCC GTATGATGTC 
GAGATCCGAC GCGGTGAAAA TGAAGGATTT GGATTTGTCA TTGTGTCTTC TGTCAGCCGG 
CCAGAAGCCG GGACTACTTT TGCCGGCAAT GCATGTGTGG CCATGCCTCA CAAAATAGGT 
CGGATAATAG AGGGGAGCCC CGCGGATCGC TGCGGCAAGC TGAAAGTGGG GGACCGGATC 
TTGGCCGTTA ACGGGTGCTC CATCACCAAC AAGTCACACT CGGACATCGT CAATTTAATA 
AAGGAAGCGG GGAACACCGT GACTCTGCGC ATCATTCCCG GCGATGAAAC GTCCAACGCG 
TCTTTATTAA CCAATGCAGA GAAGATTGCC ACGATTACGA CCACGCATAC GCCACAAACG 
ATACCCCAAG AAACAAAAAA TAATGCAAAA CCAAAGCCGG AGCCCCAGTT TGAATTCAAG 
AACTCACCAG CCACACAGCC TGATCAAGAA ATTTATTCAG TGGAACTAGA CCGAGGATTA 
AAAGGATTTG GGTTTAGTCT ACGAGGAGGT CGAGAATATA ACATGGATCT CTATGTTTTA 
CGCCTTGCGG AGGATGGGCC TGCAGCCAGA TGTGGGAAAA TGAGGGTTGG CGATGAAATC 
CTGGAAATAA ACGGAGAGAC CACAAAGAAC ATGAAGCACG CCCGGGCAAT AGAACTGATT 
AAAAATGGTG GCCGAAGAGT ACGCCTGTGT CTGAAACGCG GAGATGGTTC CGTACCGGAA 
TATGAACCCG GCGTTGACGG AAACAGCCCC ACAACCAGTC CACAAAGTGT ATCGAAAATG 
AACTCGTTAC CATCTGATAG AAATCACTCA TCACTGGAGT TCAATTATCC ATCCGATCTT 
CACAAATCAT TACAGCATGG AGACAAACGA GCTTACGTTA AAGACCCTAA AGGCAACAGG 
GAGTATGGCA GACCACATAA CGAACATCAC ACTTGGAATG GATCTTCTAA AAATTATTCT 
GTACAAAATA ACAGAAAAGA TCGATCACCT GAGGGGAGGC GACCAAGACA GGCCGATCGA 
ACAATGACGA ACGGTCAGAA AAGACAGTCT CCTGAGAAAA GAAATGAAGG AACTAGGAGT 
GCTGAGAACA CACTGGAAAG GAGAGAGAAA AATGAGAGAA GAAAAGAGGT TTCTCCTCAA 
AAGCCCAGGG AAAGGTCGCC GGTGCGCAGA AGGGATGGTT CCCCAACCAG GCGAAGGAAA 
TCCTTGGATA GATTTATGGA TCAAAGACGA TCCCCTGATA GGAGAAGAGA GAGTTCACCT 
GACCGAAGAA GAGCAAAGTC AACGGACAGG AGACGAGAAA GGTCACCAGA AAGGAGGAGA 
GAAAGGTCTC CTGAGAGAAG AAGAGAGAAG TCCACTGAGA GAAGAAGGGA CAGGTCTCCT 
GATAAACGAA ACAAAGAGCA ACGAACCAGC AACAGAGACA GGGATGAACA CAGTCTGAAA 
TATGATACCC TCAGAAGTGG CAGGCACCAA CAAGAACAAC GGAGGAGGCA ATACAAGGAA 
TGCAGCACCG ACCTGAGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757088.1
CDS	277..4680
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELGIGSPELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_031901228.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901228.1

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGGGGT ACACACCGAG GAACTGGACA GTGAACTAGG CATCGGCTCT 
CCAGAATTGC CCGCTGGATG GGAGAAAATT GAAGACCCGG TTTATGGAGT CTACTATGTA 
GATCACATAA ACAAGAAAAC GCAGTATGAA AATCCTGTAT TGGAGGCCAA ACGAAAGAAA 
CAGCTTGGAC AGCAGCAAAG TGAAGAATGG ACAGGGGACC ATCCAACCAT TGCTCCGCCC 
CCCATAATGC TTCCTAACAA CCTTACTCCA AAAAAGAAGG AACCAGCAAG AGAAACGGCA 
TCACATGTGA AACCGTTCTT CACCAGGAAC CCCTCTGAAC TGAAAGGCAA GTTCATCCAT 
ACGAAGCTAA GGAAAAGTAG TCGGGGCTTT GGCTTTACCG TTGTCGGAGG GGACGAACCA 
GATGAGTTCT TGCAAATAAA AAGTTTGGTC GTCGACGGTC CGGCCGCGTT GGACGGCAAA 
ATGGAAACGG GTGATGTTAT CGTTAGCGTG AACGATATCT GCGTCCTTGG CTACACGCAC 
GCTCAAGTTG TGAAGATCTT CCAGTCTATA CCCATTGGGG AAAGTGTGGA CCTTGAACTA 
TGCCGGGGTT ATCCTCTTCC GTTTGACCCA GATGACCCCA ACACCAGCCT TGTGACATCA 
GTTGCAATAT TGGACAAAGA CCCTATCATT GTCAATGGGC AAGAGAATTA TGACTCTCCG 
TCGAGCAACA GCAGTAAAAC TAGAAAAGCC AATAACGTCA AGGATCCAAG GCCTAGCAGC 
CCTGCTGACA TTTCTTCAAA TGGTTCCCAT GGTTACCCTA ATGATACAGT GTCCTTGGCA 
TCCTCTATAG CTACGCAACC TGAACTAATT ACTGTGCATA TAGTAAAAGG ACCAATGGGA 
TTTGGCTTTA CCATAGCAGA CAGTCCTGGC GGTGGGGGCC AGAGAGTGAA GCAGATTGTA 
GATAGTCCAA GATGCCGGGG CCTCAAAGAA GGAGATCTTA TAGTAGAGGT GAATAAAAAG 
AATGTTCAGA ACCTTACCCA TAACCAAGTT GTGGATTTAC TCATCGAATG TCCTAAGGGA 
AGCGAAGTTA CCCTACTGGT TCAAAGAGGA GGACTCCCGG TCCCAAAGAA GAGCCCAAAG 
TCGCAACCAC TTGAAAGGAA AGACAGCCAA AACAGCTCGC AGCACAGTGT TTCCAGCCAG 
CACAGTGGGC ACACTGCTTC ACCCCATCAC GCCGCGCCTG GGCTCCCCGA TTATCCTACC 
AGCGAGGCGC CGGCTGTCGA CCAAGCCGAC AACTCCGGGC AGAAAAAGCC TGATCCTTTC 
AAAATCTGGG CCCAATCCAG GAGCATGTAT GAGAACAGAC TTCCAGACTT TCAAGAGCAT 
GATATATTTT TGTGGCGAAA GGAAACTGGA TTTGGGTTTC GAATTCTCGG TGGAAATGAG 
CCTGGAGAAC CAATCTTCAT AGGTCATATT GTGCCACTGG GGTCAGCGGA TGCCGACGGG 
CGGTTGCGCT CTGGTGACGA GCTTATCTGT GTGGATGGAA CAGCGGTTGT TGGAAAATCT 
CATCAACTCG TCGTACAGCT TATGCAACAG GCTGCGAAGC AAGGACACGT CAATCTAACG 
ATCAGACGAA AAACGATCTA TCCGGTTCCA AAGGCAGAGA ACGAAGCTCC TGCATCACCA 
GCATCCTCGC ACCATAGCAG CACCCAGCCG GCGTCTCTAA CGGAGGAGAA GCGGACGCCC 
CAAGGCAGCC AAAACTCCTT AAACACTGTC AGTTCAGGCA GCGGCAGCAC CAGCGGCATT 
GGCAGTGGCG GAGGCGGCGG GAGCGGCAAC GTCAGCACCG TGGTTCAGCC GTATGATGTC 
GAGATCCGAC GCGGTGAAAA TGAAGGATTT GGATTTGTCA TTGTGTCTTC TGTCAGCCGG 
CCAGAAGCCG GGACTACTTT TGCCGGCAAT GCATGTGTGG CCATGCCTCA CAAAATAGGT 
CGGATAATAG AGGGGAGCCC CGCGGATCGC TGCGGCAAGC TGAAAGTGGG GGACCGGATC 
TTGGCCGTTA ACGGGTGCTC CATCACCAAC AAGTCACACT CGGACATCGT CAATTTAATA 
AAGGAAGCGG GGAACACCGT GACTCTGCGC ATCATTCCCG GCGATGAAAC GTCCAACGCG 
TCTTTATTAA CCAATGCAGA GAAGATTGCC ACGATTACGA CCACGCATAC GCCACAAACG 
ATACCCCAAG AAACAAAAAA TAATGCAAAA CCAAAGCCGG AGCCCCAGTT TGAATTCAAG 
AACTCACCAG CCACACAGCC TGATCAAGAA ATTTATTCAG TGGAACTAGA CCGAGGATTA 
AAAGGATTTG GGTTTAGTCT ACGAGGAGGT CGAGAATATA ACATGGATCT CTATGTTTTA 
CGCCTTGCGG AGGATGGGCC TGCAGCCAGA TGTGGGAAAA TGAGGGTTGG CGATGAAATC 
CTGGAAATAA ACGGAGAGAC CACAAAGAAC ATGAAGCACG CCCGGGCAAT AGAACTGATT 
AAAAATGGTG GCCGAAGAGT ACGCCTGTGT CTGAAACGCG GAGATGGTTC CGTACCGGAA 
TATGAACCCG GCGTTGACGG AAACAGCCCC ACAACCAGTC CACAAAGTGT ATCGAAAATG 
AACTCGTTAC CATCTGATAG AAATCACTCA TCACTGGAGT TCAATTATCC ATCCGATCTT 
CACAAATCAT TACAGCATGG AGACAAACGA GCTTACGTTA AAGACCCTAA AGGCAACAGG 
GAGTATGGCA GACCACATAA CGAACATCAC ACTTGGAATG GATCTTCTAA AAATTATTCT 
GTACAAAATA ACAGAAAAGA TCGATCACCT GAGGGGAGGC GACCAAGACA GGCCGATCGA 
ACAATGACGA ACGGTCAGAA AAGACAGTCT CCTGAGAAAA GAAATGAAGG AACTAGGAGT 
GCTGAGAACA CACTGGAAAG GAGAGAGAAA AATGAGAGAA GAAAAGAGGT TTCTCCTCAA 
AAGCCCAGGG AAAGGTCGCC GGTGCGCAGA AGGGATGGTT CCCCAACCAG GCGAAGGAAA 
TCCTTGGATA GATTTATGGA TCAAAGACGA TCCCCTGATA GGAGAAGAGA GAGTTCACCT 
GACCGAAGAA GAGCAAAGTC AACGGACAGG AGACGAGAAA GGTCACCAGA AAGGAGGAGA 
GAAAGGTCTC CTGAGAGAAG AAGAGAGAAG TCCACTGAGA GAAGAAGGGA CAGGTCTCCT 
GATAAACGAA ACAAAGAGCA ACGAACCAGC AACAGAGACA GGGATGAACA CAGTCTGAAA 
TATGATACCC TCAGAAGTGG CAGGCACCAA CAAGAACAAC GGAGGAGGCA ATACAAGGAA 
TGCAGCACCG ACCTGAGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901229.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757089.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa57626
Clone ID Related Accession (Same CDS sequence)	XM_031901229.1
Accession Version	XM_031901229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGAATTGCCC 
GCTGGATGGG AGAAAATTGA AGACCCGGTT TATGGAGTCT ACTATGTAGA TCACATAAAC 
AAGAAAACGC AGTATGAAAA TCCTGTATTG GAGGCCAAAC GAAAGAAACA GCTTGGACAG 
CAGCAAAGTG AAGAATGGAC AGGGGACCAT CCAACCATTG CTCCGCCCCC CATAATGCTT 
CCTAACAACC TTACTCCAAA AAAGAAGGAA CCAGCAAGAG AAACGGCATC ACATGTGAAA 
CCGTTCTTCA CCAGGAACCC CTCTGAACTG AAAGGCAAGT TCATCCATAC GAAGCTAAGG 
AAAAGTAGTC GGGGCTTTGG CTTTACCGTT GTCGGAGGGG ACGAACCAGA TGAGTTCTTG 
CAAATAAAAA GTTTGGTCGT CGACGGTCCG GCCGCGTTGG ACGGCAAAAT GGAAACGGGT 
GATGTTATCG TTAGCGTGAA CGATATCTGC GTCCTTGGCT ACACGCACGC TCAAGTTGTG 
AAGATCTTCC AGTCTATACC CATTGGGGAA AGTGTGGACC TTGAACTATG CCGGGGTTAT 
CCTCTTCCGT TTGACCCAGA TGACCCCAAC ACCAGCCTTG TGACATCAGT TGCAATATTG 
GACAAAGACC CTATCATTGT CAATGGGCAA GAGAATTATG ACTCTCCGTC GAGCAACAGC 
AGTAAAACTA GAAAAGCCAA TAACGTCAAG GATCCAAGGC CTAGCAGCCC TGCTGACATT 
TCTTCAAATG GTTCCCATGG TTACCCTAAT GATACAGTGT CCTTGGCATC CTCTATAGCT 
ACGCAACCTG AACTAATTAC TGTGCATATA GTAAAAGGAC CAATGGGATT TGGCTTTACC 
ATAGCAGACA GTCCTGGCGG TGGGGGCCAG AGAGTGAAGC AGATTGTAGA TAGTCCAAGA 
TGCCGGGGCC TCAAAGAAGG AGATCTTATA GTAGAGGTGA ATAAAAAGAA TGTTCAGAAC 
CTTACCCATA ACCAAGTTGT GGATTTACTC ATCGAATGTC CTAAGGGAAG CGAAGTTACC 
CTACTGGTTC AAAGAGGAGG ACTCCCGGTC CCAAAGAAGA GCCCAAAGTC GCAACCACTT 
GAAAGGAAAG ACAGCCAAAA CAGCTCGCAG CACAGTGTTT CCAGCCAGCA CAGTGGGCAC 
ACTGCTTCAC CCCATCACGC CGCGCCTGGG CTCCCCGATT ATCCTACCAG CGAGGCGCCG 
GCTGTCGACC AAGCCGACAA CTCCGGGCAG AAAAAGCCTG ATCCTTTCAA AATCTGGGCC 
CAATCCAGGA GCATGTATGA GAACAGACTT CCAGACTTTC AAGAGCATGA TATATTTTTG 
TGGCGAAAGG AAACTGGATT TGGGTTTCGA ATTCTCGGTG GAAATGAGCC TGGAGAACCA 
ATCTTCATAG GTCATATTGT GCCACTGGGG TCAGCGGATG CCGACGGGCG GTTGCGCTCT 
GGTGACGAGC TTATCTGTGT GGATGGAACA GCGGTTGTTG GAAAATCTCA TCAACTCGTC 
GTACAGCTTA TGCAACAGGC TGCGAAGCAA GGACACGTCA ATCTAACGAT CAGACGAAAA 
ACGATCTATC CGGTTCCAAA GGCAGAGAAC GAAGCTCCTG CATCACCAGC ATCCTCGCAC 
CATAGCAGCA CCCAGCCGGC GTCTCTAACG GAGGAGAAGC GGACGCCCCA AGGCAGCCAA 
AACTCCTTAA ACACTGTCAG TTCAGGCAGC GGCAGCACCA GCGGCATTGG CAGTGGCGGA 
GGCGGCGGGA GCGGCAACGT CAGCACCGTG GTTCAGCCGT ATGATGTCGA GATCCGACGC 
GGTGAAAATG AAGGATTTGG ATTTGTCATT GTGTCTTCTG TCAGCCGGCC AGAAGCCGGG 
ACTACTTTTG CCGGCAATGC ATGTGTGGCC ATGCCTCACA AAATAGGTCG GATAATAGAG 
GGGAGCCCCG CGGATCGCTG CGGCAAGCTG AAAGTGGGGG ACCGGATCTT GGCCGTTAAC 
GGGTGCTCCA TCACCAACAA GTCACACTCG GACATCGTCA ATTTAATAAA GGAAGCGGGG 
AACACCGTGA CTCTGCGCAT CATTCCCGGC GATGAAACGT CCAACGCGTC TTTATTAACC 
AATGCAGAGA AGATTGCCAC GATTACGACC ACGCATACGC CACAAACGAT ACCCCAAGAA 
ACAAAAAATA ATGCAAAACC AAAGCCGGAG CCCCAGTTTG AATTCAAGAA CTCACCAGCC 
ACACAGCCTG ATCAAGAAAT TTATTCAGTG GAACTAGACC GAGGATTAAA AGGATTTGGG 
TTTAGTCTAC GAGGAGGTCG AGAATATAAC ATGGATCTCT ATGTTTTACG CCTTGCGGAG 
GATGGGCCTG CAGCCAGATG TGGGAAAATG AGGGTTGGCG ATGAAATCCT GGAAATAAAC 
GGAGAGACCA CAAAGAACAT GAAGCACGCC CGGGCAATAG AACTGATTAA AAATGGTGGC 
CGAAGAGTAC GCCTGTGTCT GAAACGCGGA GATGGTTCCG TACCGGAATA TGAACCCGGC 
GTTGACGGAA ACAGCCCCAC AACCAGTCCA CAAAGTGTAT CGAAAATGAA CTCGTTACCA 
TCTGATAGAA ATCACTCATC ACTGGAGTTC AATTATCCAT CCGATCTTCA CAAATCATTA 
CAGCATGGAG ACAAACGAGC TTACGTTAAA GACCCTAAAG GCAACAGGGA GTATGGCAGA 
CCACATAACG AACATCACAC TTGGAATGGA TCTTCTAAAA ATTATTCTGT ACAAAATAAC 
AGAAAAGATC GATCACCTGA GGGGAGGCGA CCAAGACAGG CCGATCGAAC AATGACGAAC 
GGTCAGAAAA GACAGTCTCC TGAGAAAAGA AATGAAGGAA CTAGGAGTGC TGAGAACACA 
CTGGAAAGGA GAGAGAAAAA TGAGAGAAGA AAAGAGGTTT CTCCTCAAAA GCCCAGGGAA 
AGGTCGCCGG TGCGCAGAAG GGATGGTTCC CCAACCAGGC GAAGGAAATC CTTGGATAGA 
TTTATGGATC AAAGACGATC CCCTGATAGG AGAAGAGAGA GTTCACCTGA CCGAAGAAGA 
GCAAAGTCAA CGGACAGGAG ACGAGAAAGG TCACCAGAAA GGAGGAGAGA AAGGTCTCCT 
GAGAGAAGAA GAGAGAAGTC CACTGAGAGA AGAAGGGACA GGTCTCCTGA TAAACGAAAC 
AAAGAGCAAC GAACCAGCAA CAGAGACAGG GATGAACACA GTCTGAAATA TGATACCCTC 
AGAAGTGGCA GGCACCAACA AGAACAACGG AGGAGGCAAT ACAAGGAATG CAGCACCGAC 
CTGAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757089.1
CDS	277..4668
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_031901229.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901229.1

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGAATTGCCC 
GCTGGATGGG AGAAAATTGA AGACCCGGTT TATGGAGTCT ACTATGTAGA TCACATAAAC 
AAGAAAACGC AGTATGAAAA TCCTGTATTG GAGGCCAAAC GAAAGAAACA GCTTGGACAG 
CAGCAAAGTG AAGAATGGAC AGGGGACCAT CCAACCATTG CTCCGCCCCC CATAATGCTT 
CCTAACAACC TTACTCCAAA AAAGAAGGAA CCAGCAAGAG AAACGGCATC ACATGTGAAA 
CCGTTCTTCA CCAGGAACCC CTCTGAACTG AAAGGCAAGT TCATCCATAC GAAGCTAAGG 
AAAAGTAGTC GGGGCTTTGG CTTTACCGTT GTCGGAGGGG ACGAACCAGA TGAGTTCTTG 
CAAATAAAAA GTTTGGTCGT CGACGGTCCG GCCGCGTTGG ACGGCAAAAT GGAAACGGGT 
GATGTTATCG TTAGCGTGAA CGATATCTGC GTCCTTGGCT ACACGCACGC TCAAGTTGTG 
AAGATCTTCC AGTCTATACC CATTGGGGAA AGTGTGGACC TTGAACTATG CCGGGGTTAT 
CCTCTTCCGT TTGACCCAGA TGACCCCAAC ACCAGCCTTG TGACATCAGT TGCAATATTG 
GACAAAGACC CTATCATTGT CAATGGGCAA GAGAATTATG ACTCTCCGTC GAGCAACAGC 
AGTAAAACTA GAAAAGCCAA TAACGTCAAG GATCCAAGGC CTAGCAGCCC TGCTGACATT 
TCTTCAAATG GTTCCCATGG TTACCCTAAT GATACAGTGT CCTTGGCATC CTCTATAGCT 
ACGCAACCTG AACTAATTAC TGTGCATATA GTAAAAGGAC CAATGGGATT TGGCTTTACC 
ATAGCAGACA GTCCTGGCGG TGGGGGCCAG AGAGTGAAGC AGATTGTAGA TAGTCCAAGA 
TGCCGGGGCC TCAAAGAAGG AGATCTTATA GTAGAGGTGA ATAAAAAGAA TGTTCAGAAC 
CTTACCCATA ACCAAGTTGT GGATTTACTC ATCGAATGTC CTAAGGGAAG CGAAGTTACC 
CTACTGGTTC AAAGAGGAGG ACTCCCGGTC CCAAAGAAGA GCCCAAAGTC GCAACCACTT 
GAAAGGAAAG ACAGCCAAAA CAGCTCGCAG CACAGTGTTT CCAGCCAGCA CAGTGGGCAC 
ACTGCTTCAC CCCATCACGC CGCGCCTGGG CTCCCCGATT ATCCTACCAG CGAGGCGCCG 
GCTGTCGACC AAGCCGACAA CTCCGGGCAG AAAAAGCCTG ATCCTTTCAA AATCTGGGCC 
CAATCCAGGA GCATGTATGA GAACAGACTT CCAGACTTTC AAGAGCATGA TATATTTTTG 
TGGCGAAAGG AAACTGGATT TGGGTTTCGA ATTCTCGGTG GAAATGAGCC TGGAGAACCA 
ATCTTCATAG GTCATATTGT GCCACTGGGG TCAGCGGATG CCGACGGGCG GTTGCGCTCT 
GGTGACGAGC TTATCTGTGT GGATGGAACA GCGGTTGTTG GAAAATCTCA TCAACTCGTC 
GTACAGCTTA TGCAACAGGC TGCGAAGCAA GGACACGTCA ATCTAACGAT CAGACGAAAA 
ACGATCTATC CGGTTCCAAA GGCAGAGAAC GAAGCTCCTG CATCACCAGC ATCCTCGCAC 
CATAGCAGCA CCCAGCCGGC GTCTCTAACG GAGGAGAAGC GGACGCCCCA AGGCAGCCAA 
AACTCCTTAA ACACTGTCAG TTCAGGCAGC GGCAGCACCA GCGGCATTGG CAGTGGCGGA 
GGCGGCGGGA GCGGCAACGT CAGCACCGTG GTTCAGCCGT ATGATGTCGA GATCCGACGC 
GGTGAAAATG AAGGATTTGG ATTTGTCATT GTGTCTTCTG TCAGCCGGCC AGAAGCCGGG 
ACTACTTTTG CCGGCAATGC ATGTGTGGCC ATGCCTCACA AAATAGGTCG GATAATAGAG 
GGGAGCCCCG CGGATCGCTG CGGCAAGCTG AAAGTGGGGG ACCGGATCTT GGCCGTTAAC 
GGGTGCTCCA TCACCAACAA GTCACACTCG GACATCGTCA ATTTAATAAA GGAAGCGGGG 
AACACCGTGA CTCTGCGCAT CATTCCCGGC GATGAAACGT CCAACGCGTC TTTATTAACC 
AATGCAGAGA AGATTGCCAC GATTACGACC ACGCATACGC CACAAACGAT ACCCCAAGAA 
ACAAAAAATA ATGCAAAACC AAAGCCGGAG CCCCAGTTTG AATTCAAGAA CTCACCAGCC 
ACACAGCCTG ATCAAGAAAT TTATTCAGTG GAACTAGACC GAGGATTAAA AGGATTTGGG 
TTTAGTCTAC GAGGAGGTCG AGAATATAAC ATGGATCTCT ATGTTTTACG CCTTGCGGAG 
GATGGGCCTG CAGCCAGATG TGGGAAAATG AGGGTTGGCG ATGAAATCCT GGAAATAAAC 
GGAGAGACCA CAAAGAACAT GAAGCACGCC CGGGCAATAG AACTGATTAA AAATGGTGGC 
CGAAGAGTAC GCCTGTGTCT GAAACGCGGA GATGGTTCCG TACCGGAATA TGAACCCGGC 
GTTGACGGAA ACAGCCCCAC AACCAGTCCA CAAAGTGTAT CGAAAATGAA CTCGTTACCA 
TCTGATAGAA ATCACTCATC ACTGGAGTTC AATTATCCAT CCGATCTTCA CAAATCATTA 
CAGCATGGAG ACAAACGAGC TTACGTTAAA GACCCTAAAG GCAACAGGGA GTATGGCAGA 
CCACATAACG AACATCACAC TTGGAATGGA TCTTCTAAAA ATTATTCTGT ACAAAATAAC 
AGAAAAGATC GATCACCTGA GGGGAGGCGA CCAAGACAGG CCGATCGAAC AATGACGAAC 
GGTCAGAAAA GACAGTCTCC TGAGAAAAGA AATGAAGGAA CTAGGAGTGC TGAGAACACA 
CTGGAAAGGA GAGAGAAAAA TGAGAGAAGA AAAGAGGTTT CTCCTCAAAA GCCCAGGGAA 
AGGTCGCCGG TGCGCAGAAG GGATGGTTCC CCAACCAGGC GAAGGAAATC CTTGGATAGA 
TTTATGGATC AAAGACGATC CCCTGATAGG AGAAGAGAGA GTTCACCTGA CCGAAGAAGA 
GCAAAGTCAA CGGACAGGAG ACGAGAAAGG TCACCAGAAA GGAGGAGAGA AAGGTCTCCT 
GAGAGAAGAA GAGAGAAGTC CACTGAGAGA AGAAGGGACA GGTCTCCTGA TAAACGAAAC 
AAAGAGCAAC GAACCAGCAA CAGAGACAGG GATGAACACA GTCTGAAATA TGATACCCTC 
AGAAGTGGCA GGCACCAACA AGAACAACGG AGGAGGCAAT ACAAGGAATG CAGCACCGAC 
CTGAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901230.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757090.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa57627
Clone ID Related Accession (Same CDS sequence)	XM_031901230.1
Accession Version	XM_031901230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGGGGT ACACACCGAG GAACTGGACA GTGAACTAGA ATTGCCCGCT 
GGATGGGAGA AAATTGAAGA CCCGGTTTAT GGAGTCTACT ATGTAGATCA CATAAACAAG 
AAAACGCAGT ATGAAAATCC TGTATTGGAG GCCAAACGAA AGAAACAGCT TGGACAGCAG 
CAAAGTGAAG AATGGACAGG GGACCATCCA ACCATTGCTC CGCCCCCCAT AATGCTTCCT 
AACAACCTTA CTCCAAAAAA GAAGGAACCA GCAAGAGAAA CGGCATCACA TGTGAAACCG 
TTCTTCACCA GGAACCCCTC TGAACTGAAA GGCAAGTTCA TCCATACGAA GCTAAGGAAA 
AGTAGTCGGG GCTTTGGCTT TACCGTTGTC GGAGGGGACG AACCAGATGA GTTCTTGCAA 
ATAAAAAGTT TGGTCGTCGA CGGTCCGGCC GCGTTGGACG GCAAAATGGA AACGGGTGAT 
GTTATCGTTA GCGTGAACGA TATCTGCGTC CTTGGCTACA CGCACGCTCA AGTTGTGAAG 
ATCTTCCAGT CTATACCCAT TGGGGAAAGT GTGGACCTTG AACTATGCCG GGGTTATCCT 
CTTCCGTTTG ACCCAGATGA CCCCAACACC AGCCTTGTGA CATCAGTTGC AATATTGGAC 
AAAGACCCTA TCATTGTCAA TGGGCAAGAG AATTATGACT CTCCGTCGAG CAACAGCAGT 
AAAACTAGAA AAGCCAATAA CGTCAAGGAT CCAAGGCCTA GCAGCCCTGC TGACATTTCT 
TCAAATGGTT CCCATGGTTA CCCTAATGAT ACAGTGTCCT TGGCATCCTC TATAGCTACG 
CAACCTGAAC TAATTACTGT GCATATAGTA AAAGGACCAA TGGGATTTGG CTTTACCATA 
GCAGACAGTC CTGGCGGTGG GGGCCAGAGA GTGAAGCAGA TTGTAGATAG TCCAAGATGC 
CGGGGCCTCA AAGAAGGAGA TCTTATAGTA GAGGTGAATA AAAAGAATGT TCAGAACCTT 
ACCCATAACC AAGTTGTGGA TTTACTCATC GAATGTCCTA AGGGAAGCGA AGTTACCCTA 
CTGGTTCAAA GAGGAGGACT CCCGGTCCCA AAGAAGAGCC CAAAGTCGCA ACCACTTGAA 
AGGAAAGACA GCCAAAACAG CTCGCAGCAC AGTGTTTCCA GCCAGCACAG TGGGCACACT 
GCTTCACCCC ATCACGCCGC GCCTGGGCTC CCCGATTATC CTACCAGCGA GGCGCCGGCT 
GTCGACCAAG CCGACAACTC CGGGCAGAAA AAGCCTGATC CTTTCAAAAT CTGGGCCCAA 
TCCAGGAGCA TGTATGAGAA CAGACTTCCA GACTTTCAAG AGCATGATAT ATTTTTGTGG 
CGAAAGGAAA CTGGATTTGG GTTTCGAATT CTCGGTGGAA ATGAGCCTGG AGAACCAATC 
TTCATAGGTC ATATTGTGCC ACTGGGGTCA GCGGATGCCG ACGGGCGGTT GCGCTCTGGT 
GACGAGCTTA TCTGTGTGGA TGGAACAGCG GTTGTTGGAA AATCTCATCA ACTCGTCGTA 
CAGCTTATGC AACAGGCTGC GAAGCAAGGA CACGTCAATC TAACGATCAG ACGAAAAACG 
ATCTATCCGG TTCCAAAGGC AGAGAACGAA GCTCCTGCAT CACCAGCATC CTCGCACCAT 
AGCAGCACCC AGCCGGCGTC TCTAACGGAG GAGAAGCGGA CGCCCCAAGG CAGCCAAAAC 
TCCTTAAACA CTGTCAGTTC AGGCAGCGGC AGCACCAGCG GCATTGGCAG TGGCGGAGGC 
GGCGGGAGCG GCAACGTCAG CACCGTGGTT CAGCCGTATG ATGTCGAGAT CCGACGCGGT 
GAAAATGAAG GATTTGGATT TGTCATTGTG TCTTCTGTCA GCCGGCCAGA AGCCGGGACT 
ACTTTTGCCG GCAATGCATG TGTGGCCATG CCTCACAAAA TAGGTCGGAT AATAGAGGGG 
AGCCCCGCGG ATCGCTGCGG CAAGCTGAAA GTGGGGGACC GGATCTTGGC CGTTAACGGG 
TGCTCCATCA CCAACAAGTC ACACTCGGAC ATCGTCAATT TAATAAAGGA AGCGGGGAAC 
ACCGTGACTC TGCGCATCAT TCCCGGCGAT GAAACGTCCA ACGCGTCTTT ATTAACCAAT 
GCAGAGAAGA TTGCCACGAT TACGACCACG CATACGCCAC AAACGATACC CCAAGAAACA 
AAAAATAATG CAAAACCAAA GCCGGAGCCC CAGTTTGAAT TCAAGAACTC ACCAGCCACA 
CAGCCTGATC AAGAAATTTA TTCAGTGGAA CTAGACCGAG GATTAAAAGG ATTTGGGTTT 
AGTCTACGAG GAGGTCGAGA ATATAACATG GATCTCTATG TTTTACGCCT TGCGGAGGAT 
GGGCCTGCAG CCAGATGTGG GAAAATGAGG GTTGGCGATG AAATCCTGGA AATAAACGGA 
GAGACCACAA AGAACATGAA GCACGCCCGG GCAATAGAAC TGATTAAAAA TGGTGGCCGA 
AGAGTACGCC TGTGTCTGAA ACGCGGAGAT GGTTCCGTAC CGGAATATGA ACCCGGCGTT 
GACGGAAACA GCCCCACAAC CAGTCCACAA AGTGTATCGA AAATGAACTC GTTACCATCT 
GATAGAAATC ACTCATCACT GGAGTTCAAT TATCCATCCG ATCTTCACAA ATCATTACAG 
CATGGAGACA AACGAGCTTA CGTTAAAGAC CCTAAAGGCA ACAGGGAGTA TGGCAGACCA 
CATAACGAAC ATCACACTTG GAATGGATCT TCTAAAAATT ATTCTGTACA AAATAACAGA 
AAAGATCGAT CACCTGAGGG GAGGCGACCA AGACAGGCCG ATCGAACAAT GACGAACGGT 
CAGAAAAGAC AGTCTCCTGA GAAAAGAAAT GAAGGAACTA GGAGTGCTGA GAACACACTG 
GAAAGGAGAG AGAAAAATGA GAGAAGAAAA GAGGTTTCTC CTCAAAAGCC CAGGGAAAGG 
TCGCCGGTGC GCAGAAGGGA TGGTTCCCCA ACCAGGCGAA GGAAATCCTT GGATAGATTT 
ATGGATCAAA GACGATCCCC TGATAGGAGA AGAGAGAGTT CACCTGACCG AAGAAGAGCA 
AAGTCAACGG ACAGGAGACG AGAAAGGTCA CCAGAAAGGA GGAGAGAAAG GTCTCCTGAG 
AGAAGAAGAG AGAAGTCCAC TGAGAGAAGA AGGGACAGGT CTCCTGATAA ACGAAACAAA 
GAGCAACGAA CCAGCAACAG AGACAGGGAT GAACACAGTC TGAAATATGA TACCCTCAGA 
AGTGGCAGGC ACCAACAAGA ACAACGGAGG AGGCAATACA AGGAATGCAG CACCGACCTG 
AGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757090.1
CDS	277..4665
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_031901230.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901230.1

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGGGGT ACACACCGAG GAACTGGACA GTGAACTAGA ATTGCCCGCT 
GGATGGGAGA AAATTGAAGA CCCGGTTTAT GGAGTCTACT ATGTAGATCA CATAAACAAG 
AAAACGCAGT ATGAAAATCC TGTATTGGAG GCCAAACGAA AGAAACAGCT TGGACAGCAG 
CAAAGTGAAG AATGGACAGG GGACCATCCA ACCATTGCTC CGCCCCCCAT AATGCTTCCT 
AACAACCTTA CTCCAAAAAA GAAGGAACCA GCAAGAGAAA CGGCATCACA TGTGAAACCG 
TTCTTCACCA GGAACCCCTC TGAACTGAAA GGCAAGTTCA TCCATACGAA GCTAAGGAAA 
AGTAGTCGGG GCTTTGGCTT TACCGTTGTC GGAGGGGACG AACCAGATGA GTTCTTGCAA 
ATAAAAAGTT TGGTCGTCGA CGGTCCGGCC GCGTTGGACG GCAAAATGGA AACGGGTGAT 
GTTATCGTTA GCGTGAACGA TATCTGCGTC CTTGGCTACA CGCACGCTCA AGTTGTGAAG 
ATCTTCCAGT CTATACCCAT TGGGGAAAGT GTGGACCTTG AACTATGCCG GGGTTATCCT 
CTTCCGTTTG ACCCAGATGA CCCCAACACC AGCCTTGTGA CATCAGTTGC AATATTGGAC 
AAAGACCCTA TCATTGTCAA TGGGCAAGAG AATTATGACT CTCCGTCGAG CAACAGCAGT 
AAAACTAGAA AAGCCAATAA CGTCAAGGAT CCAAGGCCTA GCAGCCCTGC TGACATTTCT 
TCAAATGGTT CCCATGGTTA CCCTAATGAT ACAGTGTCCT TGGCATCCTC TATAGCTACG 
CAACCTGAAC TAATTACTGT GCATATAGTA AAAGGACCAA TGGGATTTGG CTTTACCATA 
GCAGACAGTC CTGGCGGTGG GGGCCAGAGA GTGAAGCAGA TTGTAGATAG TCCAAGATGC 
CGGGGCCTCA AAGAAGGAGA TCTTATAGTA GAGGTGAATA AAAAGAATGT TCAGAACCTT 
ACCCATAACC AAGTTGTGGA TTTACTCATC GAATGTCCTA AGGGAAGCGA AGTTACCCTA 
CTGGTTCAAA GAGGAGGACT CCCGGTCCCA AAGAAGAGCC CAAAGTCGCA ACCACTTGAA 
AGGAAAGACA GCCAAAACAG CTCGCAGCAC AGTGTTTCCA GCCAGCACAG TGGGCACACT 
GCTTCACCCC ATCACGCCGC GCCTGGGCTC CCCGATTATC CTACCAGCGA GGCGCCGGCT 
GTCGACCAAG CCGACAACTC CGGGCAGAAA AAGCCTGATC CTTTCAAAAT CTGGGCCCAA 
TCCAGGAGCA TGTATGAGAA CAGACTTCCA GACTTTCAAG AGCATGATAT ATTTTTGTGG 
CGAAAGGAAA CTGGATTTGG GTTTCGAATT CTCGGTGGAA ATGAGCCTGG AGAACCAATC 
TTCATAGGTC ATATTGTGCC ACTGGGGTCA GCGGATGCCG ACGGGCGGTT GCGCTCTGGT 
GACGAGCTTA TCTGTGTGGA TGGAACAGCG GTTGTTGGAA AATCTCATCA ACTCGTCGTA 
CAGCTTATGC AACAGGCTGC GAAGCAAGGA CACGTCAATC TAACGATCAG ACGAAAAACG 
ATCTATCCGG TTCCAAAGGC AGAGAACGAA GCTCCTGCAT CACCAGCATC CTCGCACCAT 
AGCAGCACCC AGCCGGCGTC TCTAACGGAG GAGAAGCGGA CGCCCCAAGG CAGCCAAAAC 
TCCTTAAACA CTGTCAGTTC AGGCAGCGGC AGCACCAGCG GCATTGGCAG TGGCGGAGGC 
GGCGGGAGCG GCAACGTCAG CACCGTGGTT CAGCCGTATG ATGTCGAGAT CCGACGCGGT 
GAAAATGAAG GATTTGGATT TGTCATTGTG TCTTCTGTCA GCCGGCCAGA AGCCGGGACT 
ACTTTTGCCG GCAATGCATG TGTGGCCATG CCTCACAAAA TAGGTCGGAT AATAGAGGGG 
AGCCCCGCGG ATCGCTGCGG CAAGCTGAAA GTGGGGGACC GGATCTTGGC CGTTAACGGG 
TGCTCCATCA CCAACAAGTC ACACTCGGAC ATCGTCAATT TAATAAAGGA AGCGGGGAAC 
ACCGTGACTC TGCGCATCAT TCCCGGCGAT GAAACGTCCA ACGCGTCTTT ATTAACCAAT 
GCAGAGAAGA TTGCCACGAT TACGACCACG CATACGCCAC AAACGATACC CCAAGAAACA 
AAAAATAATG CAAAACCAAA GCCGGAGCCC CAGTTTGAAT TCAAGAACTC ACCAGCCACA 
CAGCCTGATC AAGAAATTTA TTCAGTGGAA CTAGACCGAG GATTAAAAGG ATTTGGGTTT 
AGTCTACGAG GAGGTCGAGA ATATAACATG GATCTCTATG TTTTACGCCT TGCGGAGGAT 
GGGCCTGCAG CCAGATGTGG GAAAATGAGG GTTGGCGATG AAATCCTGGA AATAAACGGA 
GAGACCACAA AGAACATGAA GCACGCCCGG GCAATAGAAC TGATTAAAAA TGGTGGCCGA 
AGAGTACGCC TGTGTCTGAA ACGCGGAGAT GGTTCCGTAC CGGAATATGA ACCCGGCGTT 
GACGGAAACA GCCCCACAAC CAGTCCACAA AGTGTATCGA AAATGAACTC GTTACCATCT 
GATAGAAATC ACTCATCACT GGAGTTCAAT TATCCATCCG ATCTTCACAA ATCATTACAG 
CATGGAGACA AACGAGCTTA CGTTAAAGAC CCTAAAGGCA ACAGGGAGTA TGGCAGACCA 
CATAACGAAC ATCACACTTG GAATGGATCT TCTAAAAATT ATTCTGTACA AAATAACAGA 
AAAGATCGAT CACCTGAGGG GAGGCGACCA AGACAGGCCG ATCGAACAAT GACGAACGGT 
CAGAAAAGAC AGTCTCCTGA GAAAAGAAAT GAAGGAACTA GGAGTGCTGA GAACACACTG 
GAAAGGAGAG AGAAAAATGA GAGAAGAAAA GAGGTTTCTC CTCAAAAGCC CAGGGAAAGG 
TCGCCGGTGC GCAGAAGGGA TGGTTCCCCA ACCAGGCGAA GGAAATCCTT GGATAGATTT 
ATGGATCAAA GACGATCCCC TGATAGGAGA AGAGAGAGTT CACCTGACCG AAGAAGAGCA 
AAGTCAACGG ACAGGAGACG AGAAAGGTCA CCAGAAAGGA GGAGAGAAAG GTCTCCTGAG 
AGAAGAAGAG AGAAGTCCAC TGAGAGAAGA AGGGACAGGT CTCCTGATAA ACGAAACAAA 
GAGCAACGAA CCAGCAACAG AGACAGGGAT GAACACAGTC TGAAATATGA TACCCTCAGA 
AGTGGCAGGC ACCAACAAGA ACAACGGAGG AGGCAATACA AGGAATGCAG CACCGACCTG 
AGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901231.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757091.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X5, mRNA.

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CloneID	OXa57628
Clone ID Related Accession (Same CDS sequence)	XM_031901231.1
Accession Version	XM_031901231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGGCATCGGC 
TCTCCAGAAT TGCCCGCTGG ATGGGAGAAA ATTGAAGACC CGGTTTATGG AGTCTACTAT 
GTAGATCACA TAAACAAGAA AACGCAGTAT GAAAATCCTG TATTGGAGGC CAAACGAAAG 
AAACAGCTTG GACAGCAGCA AAGTGAAGAA TGGACAGGGG ACCATCCAAC CATTGCTCCG 
CCCCCCATAA TGCTTCCTAA CAACCTTACT CCAAAAAAGA AGGAACCAGC AAGAGAAACG 
GCATCACATG TGAAACCGTT CTTCACCAGG AACCCCTCTG AACTGAAAGG CAAGTTCATC 
CATACGAAGC TAAGGAAAAG TAGTCGGGGC TTTGGCTTTA CCGTTGTCGG AGGGGACGAA 
CCAGATGAGT TCTTGCAAAT AAAAAGTTTG GTCGTCGACG GTCCGGCCGC GTTGGACGGC 
AAAATGGAAA CGGGTGATGT TATCGTTAGC GTGAACGATA TCTGCGTCCT TGGCTACACG 
CACGCTCAAG TTGTGAAGAT CTTCCAGTCT ATACCCATTG GGGAAAGTGT GGACCTTGAA 
CTATGCCGGG GTTATCCTCT TCCGTTTGAC CCAGATGACC CCAACACCAG CCTTGTGACA 
TCAGTTGCAA TATTGGACAA AGACCCTATC ATTGTCAATG GGCAAGAGAA TTATGACTCT 
CCGTCGAGCA ACAGCAGTAA AACTAGAAAA GCCAATAACG TCAAGGATCC AAGGCCTAGC 
AGCCCTGCTG ACATTTCTTC AAATGGTTCC CATGGTTACC CTAATGATAC AGTGTCCTTG 
GCATCCTCTA TAGCTACGCA ACCTGAACTA ATTACTGTGC ATATAGTAAA AGGACCAATG 
GGATTTGGCT TTACCATAGC AGACAGTCCT GGCGGTGGGG GCCAGAGAGT GAAGCAGATT 
GTAGATAGTC CAAGATGCCG GGGCCTCAAA GAAGGAGATC TTATAGTAGA GGTGAATAAA 
AAGAATGTTC AGAACCTTAC CCATAACCAA GTTGTGGATT TACTCATCGA ATGTCCTAAG 
GGAAGCGAAG TTACCCTACT GGTTCAAAGA GGAGGACTCC CGGTCCCAAA GAAGAGCCCA 
AAGTCGCAAC CACTTGAAAG GAAAGACAGC CAAAACAGCT CGCAGCACAG TGTTTCCAGC 
CAGCACAGTG GGCACACTGC TTCACCCCAT CACGCCGCGC CTGGGCTCCC CGATTATCCT 
ACCAGCGAGG CGCCGGCTGT CGACCAAGCC GACAACTCCG GGCAGAAAAA GCCTGATCCT 
TTCAAAATCT GGGCCCAATC CAGGAGCATG TATGAGAACA GACTTCCAGA CTTTCAAGAG 
CATGATATAT TTTTGTGGCG AAAGGAAACT GGATTTGGGT TTCGAATTCT CGGTGGAAAT 
GAGCCTGGAG AACCAATCTT CATAGGTCAT ATTGTGCCAC TGGGGTCAGC GGATGCCGAC 
GGGCGGTTGC GCTCTGGTGA CGAGCTTATC TGTGTGGATG GAACAGCGGT TGTTGGAAAA 
TCTCATCAAC TCGTCGTACA GCTTATGCAA CAGGCTGCGA AGCAAGGACA CGTCAATCTA 
ACGATCAGAC GAAAAACGAT CTATCCGGTT CCAAAGGCAG AGAACGAAGC TCCTGCATCA 
CCAGCATCCT CGCACCATAG CAGCACCCAG CCGGCGTCTC TAACGGAGGA GAAGCGGACG 
CCCCAAGGCA GCCAAAACTC CTTAAACACT GTCAGTTCAG GCAGCGGCAG CACCAGCGGC 
ATTGGCAGTG GCGGAGGCGG CGGGAGCGGC AACGTCAGCA CCGTGGTTCA GCCGTATGAT 
GTCGAGATCC GACGCGGTGA AAATGAAGGA TTTGGATTTG TCATTGTGTC TTCTGTCAGC 
CGGCCAGAAG CCGGGACTAC TTTTGGTCGG ATAATAGAGG GGAGCCCCGC GGATCGCTGC 
GGCAAGCTGA AAGTGGGGGA CCGGATCTTG GCCGTTAACG GGTGCTCCAT CACCAACAAG 
TCACACTCGG ACATCGTCAA TTTAATAAAG GAAGCGGGGA ACACCGTGAC TCTGCGCATC 
ATTCCCGGCG ATGAAACGTC CAACGCGTCT TTATTAACCA ATGCAGAGAA GATTGCCACG 
ATTACGACCA CGCATACGCC ACAAACGATA CCCCAAGAAA CAAAAAATAA TGCAAAACCA 
AAGCCGGAGC CCCAGTTTGA ATTCAAGAAC TCACCAGCCA CACAGCCTGA TCAAGAAATT 
TATTCAGTGG AACTAGACCG AGGATTAAAA GGATTTGGGT TTAGTCTACG AGGAGGTCGA 
GAATATAACA TGGATCTCTA TGTTTTACGC CTTGCGGAGG ATGGGCCTGC AGCCAGATGT 
GGGAAAATGA GGGTTGGCGA TGAAATCCTG GAAATAAACG GAGAGACCAC AAAGAACATG 
AAGCACGCCC GGGCAATAGA ACTGATTAAA AATGGTGGCC GAAGAGTACG CCTGTGTCTG 
AAACGCGGAG ATGGTTCCGT ACCGGAATAT GAACCCGGCG TTGACGGAAA CAGCCCCACA 
ACCAGTCCAC AAAGTGTATC GAAAATGAAC TCGTTACCAT CTGATAGAAA TCACTCATCA 
CTGGAGTTCA ATTATCCATC CGATCTTCAC AAATCATTAC AGCATGGAGA CAAACGAGCT 
TACGTTAAAG ACCCTAAAGG CAACAGGGAG TATGGCAGAC CACATAACGA ACATCACACT 
TGGAATGGAT CTTCTAAAAA TTATTCTGTA CAAAATAACA GAAAAGATCG ATCACCTGAG 
GGGAGGCGAC CAAGACAGGC CGATCGAACA ATGACGAACG GTCAGAAAAG ACAGTCTCCT 
GAGAAAAGAA ATGAAGGAAC TAGGAGTGCT GAGAACACAC TGGAAAGGAG AGAGAAAAAT 
GAGAGAAGAA AAGAGGTTTC TCCTCAAAAG CCCAGGGAAA GGTCGCCGGT GCGCAGAAGG 
GATGGTTCCC CAACCAGGCG AAGGAAATCC TTGGATAGAT TTATGGATCA AAGACGATCC 
CCTGATAGGA GAAGAGAGAG TTCACCTGAC CGAAGAAGAG CAAAGTCAAC GGACAGGAGA 
CGAGAAAGGT CACCAGAAAG GAGGAGAGAA AGGTCTCCTG AGAGAAGAAG AGAGAAGTCC 
ACTGAGAGAA GAAGGGACAG GTCTCCTGAT AAACGAAACA AAGAGCAACG AACCAGCAAC 
AGAGACAGGG ATGAACACAG TCTGAAATAT GATACCCTCA GAAGTGGCAG GCACCAACAA 
GAACAACGGA GGAGGCAATA CAAGGAATGC AGCACCGACC TGAGCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757091.1
CDS	277..4647
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEEGVHTEELDSELGIGSPELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_031901231.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901231.1

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGGCATCGGC 
TCTCCAGAAT TGCCCGCTGG ATGGGAGAAA ATTGAAGACC CGGTTTATGG AGTCTACTAT 
GTAGATCACA TAAACAAGAA AACGCAGTAT GAAAATCCTG TATTGGAGGC CAAACGAAAG 
AAACAGCTTG GACAGCAGCA AAGTGAAGAA TGGACAGGGG ACCATCCAAC CATTGCTCCG 
CCCCCCATAA TGCTTCCTAA CAACCTTACT CCAAAAAAGA AGGAACCAGC AAGAGAAACG 
GCATCACATG TGAAACCGTT CTTCACCAGG AACCCCTCTG AACTGAAAGG CAAGTTCATC 
CATACGAAGC TAAGGAAAAG TAGTCGGGGC TTTGGCTTTA CCGTTGTCGG AGGGGACGAA 
CCAGATGAGT TCTTGCAAAT AAAAAGTTTG GTCGTCGACG GTCCGGCCGC GTTGGACGGC 
AAAATGGAAA CGGGTGATGT TATCGTTAGC GTGAACGATA TCTGCGTCCT TGGCTACACG 
CACGCTCAAG TTGTGAAGAT CTTCCAGTCT ATACCCATTG GGGAAAGTGT GGACCTTGAA 
CTATGCCGGG GTTATCCTCT TCCGTTTGAC CCAGATGACC CCAACACCAG CCTTGTGACA 
TCAGTTGCAA TATTGGACAA AGACCCTATC ATTGTCAATG GGCAAGAGAA TTATGACTCT 
CCGTCGAGCA ACAGCAGTAA AACTAGAAAA GCCAATAACG TCAAGGATCC AAGGCCTAGC 
AGCCCTGCTG ACATTTCTTC AAATGGTTCC CATGGTTACC CTAATGATAC AGTGTCCTTG 
GCATCCTCTA TAGCTACGCA ACCTGAACTA ATTACTGTGC ATATAGTAAA AGGACCAATG 
GGATTTGGCT TTACCATAGC AGACAGTCCT GGCGGTGGGG GCCAGAGAGT GAAGCAGATT 
GTAGATAGTC CAAGATGCCG GGGCCTCAAA GAAGGAGATC TTATAGTAGA GGTGAATAAA 
AAGAATGTTC AGAACCTTAC CCATAACCAA GTTGTGGATT TACTCATCGA ATGTCCTAAG 
GGAAGCGAAG TTACCCTACT GGTTCAAAGA GGAGGACTCC CGGTCCCAAA GAAGAGCCCA 
AAGTCGCAAC CACTTGAAAG GAAAGACAGC CAAAACAGCT CGCAGCACAG TGTTTCCAGC 
CAGCACAGTG GGCACACTGC TTCACCCCAT CACGCCGCGC CTGGGCTCCC CGATTATCCT 
ACCAGCGAGG CGCCGGCTGT CGACCAAGCC GACAACTCCG GGCAGAAAAA GCCTGATCCT 
TTCAAAATCT GGGCCCAATC CAGGAGCATG TATGAGAACA GACTTCCAGA CTTTCAAGAG 
CATGATATAT TTTTGTGGCG AAAGGAAACT GGATTTGGGT TTCGAATTCT CGGTGGAAAT 
GAGCCTGGAG AACCAATCTT CATAGGTCAT ATTGTGCCAC TGGGGTCAGC GGATGCCGAC 
GGGCGGTTGC GCTCTGGTGA CGAGCTTATC TGTGTGGATG GAACAGCGGT TGTTGGAAAA 
TCTCATCAAC TCGTCGTACA GCTTATGCAA CAGGCTGCGA AGCAAGGACA CGTCAATCTA 
ACGATCAGAC GAAAAACGAT CTATCCGGTT CCAAAGGCAG AGAACGAAGC TCCTGCATCA 
CCAGCATCCT CGCACCATAG CAGCACCCAG CCGGCGTCTC TAACGGAGGA GAAGCGGACG 
CCCCAAGGCA GCCAAAACTC CTTAAACACT GTCAGTTCAG GCAGCGGCAG CACCAGCGGC 
ATTGGCAGTG GCGGAGGCGG CGGGAGCGGC AACGTCAGCA CCGTGGTTCA GCCGTATGAT 
GTCGAGATCC GACGCGGTGA AAATGAAGGA TTTGGATTTG TCATTGTGTC TTCTGTCAGC 
CGGCCAGAAG CCGGGACTAC TTTTGGTCGG ATAATAGAGG GGAGCCCCGC GGATCGCTGC 
GGCAAGCTGA AAGTGGGGGA CCGGATCTTG GCCGTTAACG GGTGCTCCAT CACCAACAAG 
TCACACTCGG ACATCGTCAA TTTAATAAAG GAAGCGGGGA ACACCGTGAC TCTGCGCATC 
ATTCCCGGCG ATGAAACGTC CAACGCGTCT TTATTAACCA ATGCAGAGAA GATTGCCACG 
ATTACGACCA CGCATACGCC ACAAACGATA CCCCAAGAAA CAAAAAATAA TGCAAAACCA 
AAGCCGGAGC CCCAGTTTGA ATTCAAGAAC TCACCAGCCA CACAGCCTGA TCAAGAAATT 
TATTCAGTGG AACTAGACCG AGGATTAAAA GGATTTGGGT TTAGTCTACG AGGAGGTCGA 
GAATATAACA TGGATCTCTA TGTTTTACGC CTTGCGGAGG ATGGGCCTGC AGCCAGATGT 
GGGAAAATGA GGGTTGGCGA TGAAATCCTG GAAATAAACG GAGAGACCAC AAAGAACATG 
AAGCACGCCC GGGCAATAGA ACTGATTAAA AATGGTGGCC GAAGAGTACG CCTGTGTCTG 
AAACGCGGAG ATGGTTCCGT ACCGGAATAT GAACCCGGCG TTGACGGAAA CAGCCCCACA 
ACCAGTCCAC AAAGTGTATC GAAAATGAAC TCGTTACCAT CTGATAGAAA TCACTCATCA 
CTGGAGTTCA ATTATCCATC CGATCTTCAC AAATCATTAC AGCATGGAGA CAAACGAGCT 
TACGTTAAAG ACCCTAAAGG CAACAGGGAG TATGGCAGAC CACATAACGA ACATCACACT 
TGGAATGGAT CTTCTAAAAA TTATTCTGTA CAAAATAACA GAAAAGATCG ATCACCTGAG 
GGGAGGCGAC CAAGACAGGC CGATCGAACA ATGACGAACG GTCAGAAAAG ACAGTCTCCT 
GAGAAAAGAA ATGAAGGAAC TAGGAGTGCT GAGAACACAC TGGAAAGGAG AGAGAAAAAT 
GAGAGAAGAA AAGAGGTTTC TCCTCAAAAG CCCAGGGAAA GGTCGCCGGT GCGCAGAAGG 
GATGGTTCCC CAACCAGGCG AAGGAAATCC TTGGATAGAT TTATGGATCA AAGACGATCC 
CCTGATAGGA GAAGAGAGAG TTCACCTGAC CGAAGAAGAG CAAAGTCAAC GGACAGGAGA 
CGAGAAAGGT CACCAGAAAG GAGGAGAGAA AGGTCTCCTG AGAGAAGAAG AGAGAAGTCC 
ACTGAGAGAA GAAGGGACAG GTCTCCTGAT AAACGAAACA AAGAGCAACG AACCAGCAAC 
AGAGACAGGG ATGAACACAG TCTGAAATAT GATACCCTCA GAAGTGGCAG GCACCAACAA 
GAACAACGGA GGAGGCAATA CAAGGAATGC AGCACCGACC TGAGCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901232.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757092.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X6, mRNA.

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CloneID	OXa57629
Clone ID Related Accession (Same CDS sequence)	XM_031901232.1
Accession Version	XM_031901232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGGGGT ACACACCGAG GAACTGGACA GTGAACTAGA ATTGCCCGCT 
GGATGGGAGA AAATTGAAGA CCCGGTTTAT GGAGTCTACT ATGTAGATCA CATAAACAAG 
AAAACGCAGT ATGAAAATCC TGTATTGGAG GCCAAACGAA AGAAACAGCT TGGACAGCAG 
CAAAGTGAAG AATGGACAGG GGACCATCCA ACCATTGCTC CGCCCCCCAT AATGCTTCCT 
AACAACCTTA CTCCAAAAAA GAAGGAACCA GCAAGAGAAA CGGCATCACA TGTGAAACCG 
TTCTTCACCA GGAACCCCTC TGAACTGAAA GGCAAGTTCA TCCATACGAA GCTAAGGAAA 
AGTAGTCGGG GCTTTGGCTT TACCGTTGTC GGAGGGGACG AACCAGATGA GTTCTTGCAA 
ATAAAAAGTT TGGTCGTCGA CGGTCCGGCC GCGTTGGACG GCAAAATGGA AACGGGTGAT 
GTTATCGTTA GCGTGAACGA TATCTGCGTC CTTGGCTACA CGCACGCTCA AGTTGTGAAG 
ATCTTCCAGT CTATACCCAT TGGGGAAAGT GTGGACCTTG AACTATGCCG GGGTTATCCT 
CTTCCGTTTG ACCCAGATGA CCCCAACACC AGCCTTGTGA CATCAGTTGC AATATTGGAC 
AAAGACCCTA TCATTGTCAA TGGGCAAGAG AATTATGACT CTCCGTCGAG CAACAGCAGT 
AAAACTAGAA AAGCCAATAA CGTCAAGGAT CCAAGGCCTA GCAGCCCTGC TGACATTTCT 
TCAAATGGTT CCCATGGTTA CCCTAATGAT ACAGTGTCCT TGGCATCCTC TATAGCTACG 
CAACCTGAAC TAATTACTGT GCATATAGTA AAAGGACCAA TGGGATTTGG CTTTACCATA 
GCAGACAGTC CTGGCGGTGG GGGCCAGAGA GTGAAGCAGA TTGTAGATAG TCCAAGATGC 
CGGGGCCTCA AAGAAGGAGA TCTTATAGTA GAGGTGAATA AAAAGAATGT TCAGAACCTT 
ACCCATAACC AAGTTGTGGA TTTACTCATC GAATGTCCTA AGGGAAGCGA AGTTACCCTA 
CTGGTTCAAA GAGGAGGACT CCCGGTCCCA AAGAAGAGCC CAAAGTCGCA ACCACTTGAA 
AGGAAAGACA GCCAAAACAG CTCGCAGCAC AGTGTTTCCA GCCAGCACAG TGGGCACACT 
GCTTCACCCC ATCACGCCGC GCCTGGGCTC CCCGATTATC CTACCAGCGA GGCGCCGGCT 
GTCGACCAAG CCGACAACTC CGGGCAGAAA AAGCCTGATC CTTTCAAAAT CTGGGCCCAA 
TCCAGGAGCA TGTATGAGAA CAGACTTCCA GACTTTCAAG AGCATGATAT ATTTTTGTGG 
CGAAAGGAAA CTGGATTTGG GTTTCGAATT CTCGGTGGAA ATGAGCCTGG AGAACCAATC 
TTCATAGGTC ATATTGTGCC ACTGGGGTCA GCGGATGCCG ACGGGCGGTT GCGCTCTGGT 
GACGAGCTTA TCTGTGTGGA TGGAACAGCG GTTGTTGGAA AATCTCATCA ACTCGTCGTA 
CAGCTTATGC AACAGGCTGC GAAGCAAGGA CACGTCAATC TAACGATCAG ACGAAAAACG 
ATCTATCCGG TTCCAAAGGC AGAGAACGAA GCTCCTGCAT CACCAGCATC CTCGCACCAT 
AGCAGCACCC AGCCGGCGTC TCTAACGGAG GAGAAGCGGA CGCCCCAAGG CAGCCAAAAC 
TCCTTAAACA CTGTCAGTTC AGGCAGCGGC AGCACCAGCG GCATTGGCAG TGGCGGAGGC 
GGCGGGAGCG GCAACGTCAG CACCGTGGTT CAGCCGTATG ATGTCGAGAT CCGACGCGGT 
GAAAATGAAG GATTTGGATT TGTCATTGTG TCTTCTGTCA GCCGGCCAGA AGCCGGGACT 
ACTTTTGGTC GGATAATAGA GGGGAGCCCC GCGGATCGCT GCGGCAAGCT GAAAGTGGGG 
GACCGGATCT TGGCCGTTAA CGGGTGCTCC ATCACCAACA AGTCACACTC GGACATCGTC 
AATTTAATAA AGGAAGCGGG GAACACCGTG ACTCTGCGCA TCATTCCCGG CGATGAAACG 
TCCAACGCGT CTTTATTAAC CAATGCAGAG AAGATTGCCA CGATTACGAC CACGCATACG 
CCACAAACGA TACCCCAAGA AACAAAAAAT AATGCAAAAC CAAAGCCGGA GCCCCAGTTT 
GAATTCAAGA ACTCACCAGC CACACAGCCT GATCAAGAAA TTTATTCAGT GGAACTAGAC 
CGAGGATTAA AAGGATTTGG GTTTAGTCTA CGAGGAGGTC GAGAATATAA CATGGATCTC 
TATGTTTTAC GCCTTGCGGA GGATGGGCCT GCAGCCAGAT GTGGGAAAAT GAGGGTTGGC 
GATGAAATCC TGGAAATAAA CGGAGAGACC ACAAAGAACA TGAAGCACGC CCGGGCAATA 
GAACTGATTA AAAATGGTGG CCGAAGAGTA CGCCTGTGTC TGAAACGCGG AGATGGTTCC 
GTACCGGAAT ATGAACCCGG CGTTGACGGA AACAGCCCCA CAACCAGTCC ACAAAGTGTA 
TCGAAAATGA ACTCGTTACC ATCTGATAGA AATCACTCAT CACTGGAGTT CAATTATCCA 
TCCGATCTTC ACAAATCATT ACAGCATGGA GACAAACGAG CTTACGTTAA AGACCCTAAA 
GGCAACAGGG AGTATGGCAG ACCACATAAC GAACATCACA CTTGGAATGG ATCTTCTAAA 
AATTATTCTG TACAAAATAA CAGAAAAGAT CGATCACCTG AGGGGAGGCG ACCAAGACAG 
GCCGATCGAA CAATGACGAA CGGTCAGAAA AGACAGTCTC CTGAGAAAAG AAATGAAGGA 
ACTAGGAGTG CTGAGAACAC ACTGGAAAGG AGAGAGAAAA ATGAGAGAAG AAAAGAGGTT 
TCTCCTCAAA AGCCCAGGGA AAGGTCGCCG GTGCGCAGAA GGGATGGTTC CCCAACCAGG 
CGAAGGAAAT CCTTGGATAG ATTTATGGAT CAAAGACGAT CCCCTGATAG GAGAAGAGAG 
AGTTCACCTG ACCGAAGAAG AGCAAAGTCA ACGGACAGGA GACGAGAAAG GTCACCAGAA 
AGGAGGAGAG AAAGGTCTCC TGAGAGAAGA AGAGAGAAGT CCACTGAGAG AAGAAGGGAC 
AGGTCTCCTG ATAAACGAAA CAAAGAGCAA CGAACCAGCA ACAGAGACAG GGATGAACAC 
AGTCTGAAAT ATGATACCCT CAGAAGTGGC AGGCACCAAC AAGAACAACG GAGGAGGCAA 
TACAAGGAAT GCAGCACCGA CCTGAGCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757092.1
CDS	277..4629
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_031901232.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901232.1

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGGGGT ACACACCGAG GAACTGGACA GTGAACTAGA ATTGCCCGCT 
GGATGGGAGA AAATTGAAGA CCCGGTTTAT GGAGTCTACT ATGTAGATCA CATAAACAAG 
AAAACGCAGT ATGAAAATCC TGTATTGGAG GCCAAACGAA AGAAACAGCT TGGACAGCAG 
CAAAGTGAAG AATGGACAGG GGACCATCCA ACCATTGCTC CGCCCCCCAT AATGCTTCCT 
AACAACCTTA CTCCAAAAAA GAAGGAACCA GCAAGAGAAA CGGCATCACA TGTGAAACCG 
TTCTTCACCA GGAACCCCTC TGAACTGAAA GGCAAGTTCA TCCATACGAA GCTAAGGAAA 
AGTAGTCGGG GCTTTGGCTT TACCGTTGTC GGAGGGGACG AACCAGATGA GTTCTTGCAA 
ATAAAAAGTT TGGTCGTCGA CGGTCCGGCC GCGTTGGACG GCAAAATGGA AACGGGTGAT 
GTTATCGTTA GCGTGAACGA TATCTGCGTC CTTGGCTACA CGCACGCTCA AGTTGTGAAG 
ATCTTCCAGT CTATACCCAT TGGGGAAAGT GTGGACCTTG AACTATGCCG GGGTTATCCT 
CTTCCGTTTG ACCCAGATGA CCCCAACACC AGCCTTGTGA CATCAGTTGC AATATTGGAC 
AAAGACCCTA TCATTGTCAA TGGGCAAGAG AATTATGACT CTCCGTCGAG CAACAGCAGT 
AAAACTAGAA AAGCCAATAA CGTCAAGGAT CCAAGGCCTA GCAGCCCTGC TGACATTTCT 
TCAAATGGTT CCCATGGTTA CCCTAATGAT ACAGTGTCCT TGGCATCCTC TATAGCTACG 
CAACCTGAAC TAATTACTGT GCATATAGTA AAAGGACCAA TGGGATTTGG CTTTACCATA 
GCAGACAGTC CTGGCGGTGG GGGCCAGAGA GTGAAGCAGA TTGTAGATAG TCCAAGATGC 
CGGGGCCTCA AAGAAGGAGA TCTTATAGTA GAGGTGAATA AAAAGAATGT TCAGAACCTT 
ACCCATAACC AAGTTGTGGA TTTACTCATC GAATGTCCTA AGGGAAGCGA AGTTACCCTA 
CTGGTTCAAA GAGGAGGACT CCCGGTCCCA AAGAAGAGCC CAAAGTCGCA ACCACTTGAA 
AGGAAAGACA GCCAAAACAG CTCGCAGCAC AGTGTTTCCA GCCAGCACAG TGGGCACACT 
GCTTCACCCC ATCACGCCGC GCCTGGGCTC CCCGATTATC CTACCAGCGA GGCGCCGGCT 
GTCGACCAAG CCGACAACTC CGGGCAGAAA AAGCCTGATC CTTTCAAAAT CTGGGCCCAA 
TCCAGGAGCA TGTATGAGAA CAGACTTCCA GACTTTCAAG AGCATGATAT ATTTTTGTGG 
CGAAAGGAAA CTGGATTTGG GTTTCGAATT CTCGGTGGAA ATGAGCCTGG AGAACCAATC 
TTCATAGGTC ATATTGTGCC ACTGGGGTCA GCGGATGCCG ACGGGCGGTT GCGCTCTGGT 
GACGAGCTTA TCTGTGTGGA TGGAACAGCG GTTGTTGGAA AATCTCATCA ACTCGTCGTA 
CAGCTTATGC AACAGGCTGC GAAGCAAGGA CACGTCAATC TAACGATCAG ACGAAAAACG 
ATCTATCCGG TTCCAAAGGC AGAGAACGAA GCTCCTGCAT CACCAGCATC CTCGCACCAT 
AGCAGCACCC AGCCGGCGTC TCTAACGGAG GAGAAGCGGA CGCCCCAAGG CAGCCAAAAC 
TCCTTAAACA CTGTCAGTTC AGGCAGCGGC AGCACCAGCG GCATTGGCAG TGGCGGAGGC 
GGCGGGAGCG GCAACGTCAG CACCGTGGTT CAGCCGTATG ATGTCGAGAT CCGACGCGGT 
GAAAATGAAG GATTTGGATT TGTCATTGTG TCTTCTGTCA GCCGGCCAGA AGCCGGGACT 
ACTTTTGGTC GGATAATAGA GGGGAGCCCC GCGGATCGCT GCGGCAAGCT GAAAGTGGGG 
GACCGGATCT TGGCCGTTAA CGGGTGCTCC ATCACCAACA AGTCACACTC GGACATCGTC 
AATTTAATAA AGGAAGCGGG GAACACCGTG ACTCTGCGCA TCATTCCCGG CGATGAAACG 
TCCAACGCGT CTTTATTAAC CAATGCAGAG AAGATTGCCA CGATTACGAC CACGCATACG 
CCACAAACGA TACCCCAAGA AACAAAAAAT AATGCAAAAC CAAAGCCGGA GCCCCAGTTT 
GAATTCAAGA ACTCACCAGC CACACAGCCT GATCAAGAAA TTTATTCAGT GGAACTAGAC 
CGAGGATTAA AAGGATTTGG GTTTAGTCTA CGAGGAGGTC GAGAATATAA CATGGATCTC 
TATGTTTTAC GCCTTGCGGA GGATGGGCCT GCAGCCAGAT GTGGGAAAAT GAGGGTTGGC 
GATGAAATCC TGGAAATAAA CGGAGAGACC ACAAAGAACA TGAAGCACGC CCGGGCAATA 
GAACTGATTA AAAATGGTGG CCGAAGAGTA CGCCTGTGTC TGAAACGCGG AGATGGTTCC 
GTACCGGAAT ATGAACCCGG CGTTGACGGA AACAGCCCCA CAACCAGTCC ACAAAGTGTA 
TCGAAAATGA ACTCGTTACC ATCTGATAGA AATCACTCAT CACTGGAGTT CAATTATCCA 
TCCGATCTTC ACAAATCATT ACAGCATGGA GACAAACGAG CTTACGTTAA AGACCCTAAA 
GGCAACAGGG AGTATGGCAG ACCACATAAC GAACATCACA CTTGGAATGG ATCTTCTAAA 
AATTATTCTG TACAAAATAA CAGAAAAGAT CGATCACCTG AGGGGAGGCG ACCAAGACAG 
GCCGATCGAA CAATGACGAA CGGTCAGAAA AGACAGTCTC CTGAGAAAAG AAATGAAGGA 
ACTAGGAGTG CTGAGAACAC ACTGGAAAGG AGAGAGAAAA ATGAGAGAAG AAAAGAGGTT 
TCTCCTCAAA AGCCCAGGGA AAGGTCGCCG GTGCGCAGAA GGGATGGTTC CCCAACCAGG 
CGAAGGAAAT CCTTGGATAG ATTTATGGAT CAAAGACGAT CCCCTGATAG GAGAAGAGAG 
AGTTCACCTG ACCGAAGAAG AGCAAAGTCA ACGGACAGGA GACGAGAAAG GTCACCAGAA 
AGGAGGAGAG AAAGGTCTCC TGAGAGAAGA AGAGAGAAGT CCACTGAGAG AAGAAGGGAC 
AGGTCTCCTG ATAAACGAAA CAAAGAGCAA CGAACCAGCA ACAGAGACAG GGATGAACAC 
AGTCTGAAAT ATGATACCCT CAGAAGTGGC AGGCACCAAC AAGAACAACG GAGGAGGCAA 
TACAAGGAAT GCAGCACCGA CCTGAGCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901233.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757093.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa57630
Clone ID Related Accession (Same CDS sequence)	XM_031901233.1
Accession Version	XM_031901233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAATTGCC CGCTGGATGG GAGAAAATTG AAGACCCGGT TTATGGAGTC 
TACTATGTAG ATCACATAAA CAAGAAAACG CAGTATGAAA ATCCTGTATT GGAGGCCAAA 
CGAAAGAAAC AGCTTGGACA GCAGCAAAGT GAAGAATGGA CAGGGGACCA TCCAACCATT 
GCTCCGCCCC CCATAATGCT TCCTAACAAC CTTACTCCAA AAAAGAAGGA ACCAGCAAGA 
GAAACGGCAT CACATGTGAA ACCGTTCTTC ACCAGGAACC CCTCTGAACT GAAAGGCAAG 
TTCATCCATA CGAAGCTAAG GAAAAGTAGT CGGGGCTTTG GCTTTACCGT TGTCGGAGGG 
GACGAACCAG ATGAGTTCTT GCAAATAAAA AGTTTGGTCG TCGACGGTCC GGCCGCGTTG 
GACGGCAAAA TGGAAACGGG TGATGTTATC GTTAGCGTGA ACGATATCTG CGTCCTTGGC 
TACACGCACG CTCAAGTTGT GAAGATCTTC CAGTCTATAC CCATTGGGGA AAGTGTGGAC 
CTTGAACTAT GCCGGGGTTA TCCTCTTCCG TTTGACCCAG ATGACCCCAA CACCAGCCTT 
GTGACATCAG TTGCAATATT GGACAAAGAC CCTATCATTG TCAATGGGCA AGAGAATTAT 
GACTCTCCGT CGAGCAACAG CAGTAAAACT AGAAAAGCCA ATAACGTCAA GGATCCAAGG 
CCTAGCAGCC CTGCTGACAT TTCTTCAAAT GGTTCCCATG GTTACCCTAA TGATACAGTG 
TCCTTGGCAT CCTCTATAGC TACGCAACCT GAACTAATTA CTGTGCATAT AGTAAAAGGA 
CCAATGGGAT TTGGCTTTAC CATAGCAGAC AGTCCTGGCG GTGGGGGCCA GAGAGTGAAG 
CAGATTGTAG ATAGTCCAAG ATGCCGGGGC CTCAAAGAAG GAGATCTTAT AGTAGAGGTG 
AATAAAAAGA ATGTTCAGAA CCTTACCCAT AACCAAGTTG TGGATTTACT CATCGAATGT 
CCTAAGGGAA GCGAAGTTAC CCTACTGGTT CAAAGAGGAG GACTCCCGGT CCCAAAGAAG 
AGCCCAAAGT CGCAACCACT TGAAAGGAAA GACAGCCAAA ACAGCTCGCA GCACAGTGTT 
TCCAGCCAGC ACAGTGGGCA CACTGCTTCA CCCCATCACG CCGCGCCTGG GCTCCCCGAT 
TATCCTACCA GCGAGGCGCC GGCTGTCGAC CAAGCCGACA ACTCCGGGCA GAAAAAGCCT 
GATCCTTTCA AAATCTGGGC CCAATCCAGG AGCATGTATG AGAACAGACT TCCAGACTTT 
CAAGAGCATG ATATATTTTT GTGGCGAAAG GAAACTGGAT TTGGGTTTCG AATTCTCGGT 
GGAAATGAGC CTGGAGAACC AATCTTCATA GGTCATATTG TGCCACTGGG GTCAGCGGAT 
GCCGACGGGC GGTTGCGCTC TGGTGACGAG CTTATCTGTG TGGATGGAAC AGCGGTTGTT 
GGAAAATCTC ATCAACTCGT CGTACAGCTT ATGCAACAGG CTGCGAAGCA AGGACACGTC 
AATCTAACGA TCAGACGAAA AACGATCTAT CCGGTTCCAA AGGCAGAGAA CGAAGCTCCT 
GCATCACCAG CATCCTCGCA CCATAGCAGC ACCCAGCCGG CGTCTCTAAC GGAGGAGAAG 
CGGACGCCCC AAGGCAGCCA AAACTCCTTA AACACTGTCA GTTCAGGCAG CGGCAGCACC 
AGCGGCATTG GCAGTGGCGG AGGCGGCGGG AGCGGCAACG TCAGCACCGT GGTTCAGCCG 
TATGATGTCG AGATCCGACG CGGTGAAAAT GAAGGATTTG GATTTGTCAT TGTGTCTTCT 
GTCAGCCGGC CAGAAGCCGG GACTACTTTT GCCGGCAATG CATGTGTGGC CATGCCTCAC 
AAAATAGGTC GGATAATAGA GGGGAGCCCC GCGGATCGCT GCGGCAAGCT GAAAGTGGGG 
GACCGGATCT TGGCCGTTAA CGGGTGCTCC ATCACCAACA AGTCACACTC GGACATCGTC 
AATTTAATAA AGGAAGCGGG GAACACCGTG ACTCTGCGCA TCATTCCCGG CGATGAAACG 
TCCAACGCGT CTTTATTAAC CAATGCAGAG AAGATTGCCA CGATTACGAC CACGCATACG 
CCACAAACGA TACCCCAAGA AACAAAAAAT AATGCAAAAC CAAAGCCGGA GCCCCAGTTT 
GAATTCAAGA ACTCACCAGC CACACAGCCT GATCAAGAAA TTTATTCAGT GGAACTAGAC 
CGAGGATTAA AAGGATTTGG GTTTAGTCTA CGAGGAGGTC GAGAATATAA CATGGATCTC 
TATGTTTTAC GCCTTGCGGA GGATGGGCCT GCAGCCAGAT GTGGGAAAAT GAGGGTTGGC 
GATGAAATCC TGGAAATAAA CGGAGAGACC ACAAAGAACA TGAAGCACGC CCGGGCAATA 
GAACTGATTA AAAATGGTGG CCGAAGAGTA CGCCTGTGTC TGAAACGCGG AGATGGTTCC 
GTACCGGAAT ATGAACCCGG CGTTGACGGA AACAGCCCCA CAACCAGTCC ACAAAGTGTA 
TCGAAAATGA ACTCGTTACC ATCTGATAGA AATCACTCAT CACTGGAGTT CAATTATCCA 
TCCGATCTTC ACAAATCATT ACAGCATGGA GACAAACGAG CTTACGTTAA AGACCCTAAA 
GGCAACAGGG AGTATGGCAG ACCACATAAC GAACATCACA CTTGGAATGG ATCTTCTAAA 
AATTATTCTG TACAAAATAA CAGAAAAGAT CGATCACCTG AGGGGAGGCG ACCAAGACAG 
GCCGATCGAA CAATGACGAA CGGTCAGAAA AGACAGTCTC CTGAGAAAAG AAATGAAGGA 
ACTAGGAGTG CTGAGAACAC ACTGGAAAGG AGAGAGAAAA ATGAGAGAAG AAAAGAGGTT 
TCTCCTCAAA AGCCCAGGGA AAGGTCGCCG GTGCGCAGAA GGGATGGTTC CCCAACCAGG 
CGAAGGAAAT CCTTGGATAG ATTTATGGAT CAAAGACGAT CCCCTGATAG GAGAAGAGAG 
AGTTCACCTG ACCGAAGAAG AGCAAAGTCA ACGGACAGGA GACGAGAAAG GTCACCAGAA 
AGGAGGAGAG AAAGGTCTCC TGAGAGAAGA AGAGAGAAGT CCACTGAGAG AAGAAGGGAC 
AGGTCTCCTG ATAAACGAAA CAAAGAGCAA CGAACCAGCA ACAGAGACAG GGATGAACAC 
AGTCTGAAAT ATGATACCCT CAGAAGTGGC AGGCACCAAC AAGAACAACG GAGGAGGCAA 
TACAAGGAAT GCAGCACCGA CCTGAGCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757093.1
CDS	277..4629
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_031901233.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901233.1

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAATTGCC CGCTGGATGG GAGAAAATTG AAGACCCGGT TTATGGAGTC 
TACTATGTAG ATCACATAAA CAAGAAAACG CAGTATGAAA ATCCTGTATT GGAGGCCAAA 
CGAAAGAAAC AGCTTGGACA GCAGCAAAGT GAAGAATGGA CAGGGGACCA TCCAACCATT 
GCTCCGCCCC CCATAATGCT TCCTAACAAC CTTACTCCAA AAAAGAAGGA ACCAGCAAGA 
GAAACGGCAT CACATGTGAA ACCGTTCTTC ACCAGGAACC CCTCTGAACT GAAAGGCAAG 
TTCATCCATA CGAAGCTAAG GAAAAGTAGT CGGGGCTTTG GCTTTACCGT TGTCGGAGGG 
GACGAACCAG ATGAGTTCTT GCAAATAAAA AGTTTGGTCG TCGACGGTCC GGCCGCGTTG 
GACGGCAAAA TGGAAACGGG TGATGTTATC GTTAGCGTGA ACGATATCTG CGTCCTTGGC 
TACACGCACG CTCAAGTTGT GAAGATCTTC CAGTCTATAC CCATTGGGGA AAGTGTGGAC 
CTTGAACTAT GCCGGGGTTA TCCTCTTCCG TTTGACCCAG ATGACCCCAA CACCAGCCTT 
GTGACATCAG TTGCAATATT GGACAAAGAC CCTATCATTG TCAATGGGCA AGAGAATTAT 
GACTCTCCGT CGAGCAACAG CAGTAAAACT AGAAAAGCCA ATAACGTCAA GGATCCAAGG 
CCTAGCAGCC CTGCTGACAT TTCTTCAAAT GGTTCCCATG GTTACCCTAA TGATACAGTG 
TCCTTGGCAT CCTCTATAGC TACGCAACCT GAACTAATTA CTGTGCATAT AGTAAAAGGA 
CCAATGGGAT TTGGCTTTAC CATAGCAGAC AGTCCTGGCG GTGGGGGCCA GAGAGTGAAG 
CAGATTGTAG ATAGTCCAAG ATGCCGGGGC CTCAAAGAAG GAGATCTTAT AGTAGAGGTG 
AATAAAAAGA ATGTTCAGAA CCTTACCCAT AACCAAGTTG TGGATTTACT CATCGAATGT 
CCTAAGGGAA GCGAAGTTAC CCTACTGGTT CAAAGAGGAG GACTCCCGGT CCCAAAGAAG 
AGCCCAAAGT CGCAACCACT TGAAAGGAAA GACAGCCAAA ACAGCTCGCA GCACAGTGTT 
TCCAGCCAGC ACAGTGGGCA CACTGCTTCA CCCCATCACG CCGCGCCTGG GCTCCCCGAT 
TATCCTACCA GCGAGGCGCC GGCTGTCGAC CAAGCCGACA ACTCCGGGCA GAAAAAGCCT 
GATCCTTTCA AAATCTGGGC CCAATCCAGG AGCATGTATG AGAACAGACT TCCAGACTTT 
CAAGAGCATG ATATATTTTT GTGGCGAAAG GAAACTGGAT TTGGGTTTCG AATTCTCGGT 
GGAAATGAGC CTGGAGAACC AATCTTCATA GGTCATATTG TGCCACTGGG GTCAGCGGAT 
GCCGACGGGC GGTTGCGCTC TGGTGACGAG CTTATCTGTG TGGATGGAAC AGCGGTTGTT 
GGAAAATCTC ATCAACTCGT CGTACAGCTT ATGCAACAGG CTGCGAAGCA AGGACACGTC 
AATCTAACGA TCAGACGAAA AACGATCTAT CCGGTTCCAA AGGCAGAGAA CGAAGCTCCT 
GCATCACCAG CATCCTCGCA CCATAGCAGC ACCCAGCCGG CGTCTCTAAC GGAGGAGAAG 
CGGACGCCCC AAGGCAGCCA AAACTCCTTA AACACTGTCA GTTCAGGCAG CGGCAGCACC 
AGCGGCATTG GCAGTGGCGG AGGCGGCGGG AGCGGCAACG TCAGCACCGT GGTTCAGCCG 
TATGATGTCG AGATCCGACG CGGTGAAAAT GAAGGATTTG GATTTGTCAT TGTGTCTTCT 
GTCAGCCGGC CAGAAGCCGG GACTACTTTT GCCGGCAATG CATGTGTGGC CATGCCTCAC 
AAAATAGGTC GGATAATAGA GGGGAGCCCC GCGGATCGCT GCGGCAAGCT GAAAGTGGGG 
GACCGGATCT TGGCCGTTAA CGGGTGCTCC ATCACCAACA AGTCACACTC GGACATCGTC 
AATTTAATAA AGGAAGCGGG GAACACCGTG ACTCTGCGCA TCATTCCCGG CGATGAAACG 
TCCAACGCGT CTTTATTAAC CAATGCAGAG AAGATTGCCA CGATTACGAC CACGCATACG 
CCACAAACGA TACCCCAAGA AACAAAAAAT AATGCAAAAC CAAAGCCGGA GCCCCAGTTT 
GAATTCAAGA ACTCACCAGC CACACAGCCT GATCAAGAAA TTTATTCAGT GGAACTAGAC 
CGAGGATTAA AAGGATTTGG GTTTAGTCTA CGAGGAGGTC GAGAATATAA CATGGATCTC 
TATGTTTTAC GCCTTGCGGA GGATGGGCCT GCAGCCAGAT GTGGGAAAAT GAGGGTTGGC 
GATGAAATCC TGGAAATAAA CGGAGAGACC ACAAAGAACA TGAAGCACGC CCGGGCAATA 
GAACTGATTA AAAATGGTGG CCGAAGAGTA CGCCTGTGTC TGAAACGCGG AGATGGTTCC 
GTACCGGAAT ATGAACCCGG CGTTGACGGA AACAGCCCCA CAACCAGTCC ACAAAGTGTA 
TCGAAAATGA ACTCGTTACC ATCTGATAGA AATCACTCAT CACTGGAGTT CAATTATCCA 
TCCGATCTTC ACAAATCATT ACAGCATGGA GACAAACGAG CTTACGTTAA AGACCCTAAA 
GGCAACAGGG AGTATGGCAG ACCACATAAC GAACATCACA CTTGGAATGG ATCTTCTAAA 
AATTATTCTG TACAAAATAA CAGAAAAGAT CGATCACCTG AGGGGAGGCG ACCAAGACAG 
GCCGATCGAA CAATGACGAA CGGTCAGAAA AGACAGTCTC CTGAGAAAAG AAATGAAGGA 
ACTAGGAGTG CTGAGAACAC ACTGGAAAGG AGAGAGAAAA ATGAGAGAAG AAAAGAGGTT 
TCTCCTCAAA AGCCCAGGGA AAGGTCGCCG GTGCGCAGAA GGGATGGTTC CCCAACCAGG 
CGAAGGAAAT CCTTGGATAG ATTTATGGAT CAAAGACGAT CCCCTGATAG GAGAAGAGAG 
AGTTCACCTG ACCGAAGAAG AGCAAAGTCA ACGGACAGGA GACGAGAAAG GTCACCAGAA 
AGGAGGAGAG AAAGGTCTCC TGAGAGAAGA AGAGAGAAGT CCACTGAGAG AAGAAGGGAC 
AGGTCTCCTG ATAAACGAAA CAAAGAGCAA CGAACCAGCA ACAGAGACAG GGATGAACAC 
AGTCTGAAAT ATGATACCCT CAGAAGTGGC AGGCACCAAC AAGAACAACG GAGGAGGCAA 
TACAAGGAAT GCAGCACCGA CCTGAGCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901235.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757095.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa57631
Clone ID Related Accession (Same CDS sequence)	XM_031901235.1
Accession Version	XM_031901235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGGCATCGGC 
TCTCCAGAAT TGCCCGCTGG ATGGGAGAAA ATTGAAGACC CGGTTTATGG AGTCTACTAT 
GTAGATCACA TAAACAAGAA AACGCAGTAT GAAAATCCTG TATTGGAGGC CAAACGAAAG 
AAACAGCTTG GACAGCAGCA AAGTGAAGAA TGGACAGGGG ACCATCCAAC CATTGCTCCG 
CCCCCCATAA TGCTTCCTAA CAACCTTACT CCAAAAAAGA AGGAACCAGC AAGAGAAACG 
GCATCACATG TGAAACCGTT CTTCACCAGG AACCCCTCTG AACTGAAAGG CAAGTTCATC 
CATACGAAGC TAAGGAAAAG TAGTCGGGGC TTTGGCTTTA CCGTTGTCGG AGGGGACGAA 
CCAGATGAGT TCTTGCAAAT AAAAAGTTTG GTCGTCGACG GTCCGGCCGC GTTGGACGGC 
AAAATGGAAA CGGGTGATGT TATCGTTAGC GTGAACGATA TCTGCGTCCT TGGCTACACG 
CACGCTCAAG TTGTGAAGAT CTTCCAGTCT ATACCCATTG GGGAAAGTGT GGACCTTGAA 
CTATGCCGGG GTTATCCTCT TCCGTTTGAC CCAGATGACC CCAACACCAG CCTTGTGACA 
TCAGTTGCAA TATTGGACAA AGACCCTATC ATTGTCAATG GGCAAGAGAA TTATGACTCT 
CCGTCGAGCA ACAGCAGTAA AACTAGAAAA GCCAATAACG TCAAGGATCC AAGGCCTAGC 
AGCCCTGCTG ACATTTCTTC AAATGGTTCC CATGGTTACC CTAATGATAC AGTGTCCTTG 
GCATCCTCTA TAGCTACGCA ACCTGAACTA ATTACTGTGC ATATAGTAAA AGGACCAATG 
GGATTTGGCT TTACCATAGC AGACAGTCCT GGCGGTGGGG GCCAGAGAGT GAAGCAGATT 
GTAGATAGTC CAAGATGCCG GGGCCTCAAA GAAGGAGATC TTATAGTAGA GGTGAATAAA 
AAGAATGTTC AGAACCTTAC CCATAACCAA GTTGTGGATT TACTCATCGA ATGTCCTAAG 
GGAAGCGAAG TTACCCTACT GGTTCAAAGA GGAGGACTCC CGGTCCCAAA GAAGAGCCCA 
AAGTCGCAAC CACTTGAAAG GAAAGACAGC CAAAACAGCT CGCAGCACAG TGTTTCCAGC 
CAGCACAGTG GGCACACTGC TTCACCCCAT CACGCCGCGC CTGGGCTCCC CGATTATCCT 
ACCAGCGAGG CGCCGGCTGT CGACCAAGCC GACAACTCCG GGCAGAAAAA GCCTGATCCT 
TTCAAAATCT GGGCCCAATC CAGGAGCATG TATGAGAACA GACTTCCAGA CTTTCAAGAG 
CATGATATAT TTTTGTGGCG AAAGGAAACT GGATTTGGGT TTCGAATTCT CGGTGGAAAT 
GAGCCTGGAG AACCAATCTT CATAGGTCAT ATTGTGCCAC TGGGGTCAGC GGATGCCGAC 
GGGCGGTTGC GCTCTGGTGA CGAGCTTATC TGTGTGGATG GAACAGCGGT TGTTGGAAAA 
TCTCATCAAC TCGTCGTACA GCTTATGCAA CAGGCTGCGA AGCAAGGACA CGTCAATCTA 
ACGATCAGAC GAAAAACGAT CTATCCGGTT CCAAAGGCAG AGAACGAAGC TCCTGCATCA 
CCAGCATCCT CGCACCATAG CAGCACCCAG CCGGCGTCTC TAACGGAGGA GAAGCGGACG 
CCCCAAGGCA GCCAAAACTC CTTAAACACT GTCAGTTCAG GCAGCGGCAG CACCAGCGGC 
ATTGGCAGTG GCGGAGGCGG CGGGAGCGGC AACGTCAGCA CCGTGGTTCA GCCGTATGAT 
GTCGAGATCC GACGCGGTGA AAATGAAGGA TTTGGATTTG TCATTGTGTC TTCTGTCAGC 
CGGCCAGAAG CCGGGACTAC TTTTGCCGGC AATGCATGTG TGGCCATGCC TCACAAAATA 
GGTCGGATAA TAGAGGGGAG CCCCGCGGAT CGCTGCGGCA AGCTGAAAGT GGGGGACCGG 
ATCTTGGCCG TTAACGGGTG CTCCATCACC AACAAGTCAC ACTCGGACAT CGTCAATTTA 
ATAAAGGAAG CGGGGAACAC CGTGACTCTG CGCATCATTC CCGGCGATGA AACGTCCAAC 
GCGTCTTTAT TAACCAATGC AGAGAAGATT GCCACGATTA CGACCACGCA TACGCCACAA 
ACGATACCCC AAGAAACAAA AAATAATGCA AAACCAAAGC CGGAGCCCCA GTTTGAATTC 
AAGAACTCAC CAGCCACACA GCCTGATCAA GAAATTTATT CAGTGGAACT AGACCGAGGA 
TTAAAAGGAT TTGGGTTTAG TCTACGAGGA GGTCGAGAAT ATAACATGGA TCTCTATGTT 
TTACGCCTTG CGGAGGATGG GCCTGCAGCC AGATGTGGGA AAATGAGGGT TGGCGATGAA 
ATCCTGGAAA TAAACGGAGA GACCACAAAG AACATGAAGC ACGCCCGGGC AATAGAACTG 
ATTAAAAATG GTGGCCGAAG AGTACGCCTG TGTCTGAAAC GCGGAGATGG TTCCGTACCG 
GAATATGCGA TGATTCCTCC TAATATCGCT GCATGTATGA GAAATGACAA GCTCGGGGAA 
CCTTGCTTCT ACCTAATGGG CCAAAATCAA ACTACGAACC CGGCGTTGAC GGAAACAGCC 
CCACAACCAG TCCACAAAGT GTATCGAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757095.1
CDS	278..4030
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEEGVHTEELDSELGIGSPELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYAMIPPNIAACMRNDKLGEPCFYLMGQNQTTNPALTETAPQPVHKVYRK

Target ORF information:

RefSeq Version	XM_031901235.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901235.1

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGGCATCGGC 
TCTCCAGAAT TGCCCGCTGG ATGGGAGAAA ATTGAAGACC CGGTTTATGG AGTCTACTAT 
GTAGATCACA TAAACAAGAA AACGCAGTAT GAAAATCCTG TATTGGAGGC CAAACGAAAG 
AAACAGCTTG GACAGCAGCA AAGTGAAGAA TGGACAGGGG ACCATCCAAC CATTGCTCCG 
CCCCCCATAA TGCTTCCTAA CAACCTTACT CCAAAAAAGA AGGAACCAGC AAGAGAAACG 
GCATCACATG TGAAACCGTT CTTCACCAGG AACCCCTCTG AACTGAAAGG CAAGTTCATC 
CATACGAAGC TAAGGAAAAG TAGTCGGGGC TTTGGCTTTA CCGTTGTCGG AGGGGACGAA 
CCAGATGAGT TCTTGCAAAT AAAAAGTTTG GTCGTCGACG GTCCGGCCGC GTTGGACGGC 
AAAATGGAAA CGGGTGATGT TATCGTTAGC GTGAACGATA TCTGCGTCCT TGGCTACACG 
CACGCTCAAG TTGTGAAGAT CTTCCAGTCT ATACCCATTG GGGAAAGTGT GGACCTTGAA 
CTATGCCGGG GTTATCCTCT TCCGTTTGAC CCAGATGACC CCAACACCAG CCTTGTGACA 
TCAGTTGCAA TATTGGACAA AGACCCTATC ATTGTCAATG GGCAAGAGAA TTATGACTCT 
CCGTCGAGCA ACAGCAGTAA AACTAGAAAA GCCAATAACG TCAAGGATCC AAGGCCTAGC 
AGCCCTGCTG ACATTTCTTC AAATGGTTCC CATGGTTACC CTAATGATAC AGTGTCCTTG 
GCATCCTCTA TAGCTACGCA ACCTGAACTA ATTACTGTGC ATATAGTAAA AGGACCAATG 
GGATTTGGCT TTACCATAGC AGACAGTCCT GGCGGTGGGG GCCAGAGAGT GAAGCAGATT 
GTAGATAGTC CAAGATGCCG GGGCCTCAAA GAAGGAGATC TTATAGTAGA GGTGAATAAA 
AAGAATGTTC AGAACCTTAC CCATAACCAA GTTGTGGATT TACTCATCGA ATGTCCTAAG 
GGAAGCGAAG TTACCCTACT GGTTCAAAGA GGAGGACTCC CGGTCCCAAA GAAGAGCCCA 
AAGTCGCAAC CACTTGAAAG GAAAGACAGC CAAAACAGCT CGCAGCACAG TGTTTCCAGC 
CAGCACAGTG GGCACACTGC TTCACCCCAT CACGCCGCGC CTGGGCTCCC CGATTATCCT 
ACCAGCGAGG CGCCGGCTGT CGACCAAGCC GACAACTCCG GGCAGAAAAA GCCTGATCCT 
TTCAAAATCT GGGCCCAATC CAGGAGCATG TATGAGAACA GACTTCCAGA CTTTCAAGAG 
CATGATATAT TTTTGTGGCG AAAGGAAACT GGATTTGGGT TTCGAATTCT CGGTGGAAAT 
GAGCCTGGAG AACCAATCTT CATAGGTCAT ATTGTGCCAC TGGGGTCAGC GGATGCCGAC 
GGGCGGTTGC GCTCTGGTGA CGAGCTTATC TGTGTGGATG GAACAGCGGT TGTTGGAAAA 
TCTCATCAAC TCGTCGTACA GCTTATGCAA CAGGCTGCGA AGCAAGGACA CGTCAATCTA 
ACGATCAGAC GAAAAACGAT CTATCCGGTT CCAAAGGCAG AGAACGAAGC TCCTGCATCA 
CCAGCATCCT CGCACCATAG CAGCACCCAG CCGGCGTCTC TAACGGAGGA GAAGCGGACG 
CCCCAAGGCA GCCAAAACTC CTTAAACACT GTCAGTTCAG GCAGCGGCAG CACCAGCGGC 
ATTGGCAGTG GCGGAGGCGG CGGGAGCGGC AACGTCAGCA CCGTGGTTCA GCCGTATGAT 
GTCGAGATCC GACGCGGTGA AAATGAAGGA TTTGGATTTG TCATTGTGTC TTCTGTCAGC 
CGGCCAGAAG CCGGGACTAC TTTTGCCGGC AATGCATGTG TGGCCATGCC TCACAAAATA 
GGTCGGATAA TAGAGGGGAG CCCCGCGGAT CGCTGCGGCA AGCTGAAAGT GGGGGACCGG 
ATCTTGGCCG TTAACGGGTG CTCCATCACC AACAAGTCAC ACTCGGACAT CGTCAATTTA 
ATAAAGGAAG CGGGGAACAC CGTGACTCTG CGCATCATTC CCGGCGATGA AACGTCCAAC 
GCGTCTTTAT TAACCAATGC AGAGAAGATT GCCACGATTA CGACCACGCA TACGCCACAA 
ACGATACCCC AAGAAACAAA AAATAATGCA AAACCAAAGC CGGAGCCCCA GTTTGAATTC 
AAGAACTCAC CAGCCACACA GCCTGATCAA GAAATTTATT CAGTGGAACT AGACCGAGGA 
TTAAAAGGAT TTGGGTTTAG TCTACGAGGA GGTCGAGAAT ATAACATGGA TCTCTATGTT 
TTACGCCTTG CGGAGGATGG GCCTGCAGCC AGATGTGGGA AAATGAGGGT TGGCGATGAA 
ATCCTGGAAA TAAACGGAGA GACCACAAAG AACATGAAGC ACGCCCGGGC AATAGAACTG 
ATTAAAAATG GTGGCCGAAG AGTACGCCTG TGTCTGAAAC GCGGAGATGG TTCCGTACCG 
GAATATGCGA TGATTCCTCC TAATATCGCT GCATGTATGA GAAATGACAA GCTCGGGGAA 
CCTTGCTTCT ACCTAATGGG CCAAAATCAA ACTACGAACC CGGCGTTGAC GGAAACAGCC 
CCACAACCAG TCCACAAAGT GTATCGAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901236.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757096.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X9, mRNA.

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CloneID	OXa57632
Clone ID Related Accession (Same CDS sequence)	XM_031901236.1
Accession Version	XM_031901236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG CAGGCGTAGT GCATACCGAC CACTATGAGT TTGATGATGA TGCTCCTGAA 
ATGAGCAGTA GCTTTACAGG AGACTCCAGT GAAGCAGACG AGCACACGAT TAACCTTTCC 
CAAGATAAAG TCCCACCGGC GGCTCGGATA CTCCCTACTT TTCCCATCAC GGAAGCTTCT 
CAGAATCTGC CTCATTACCT ACCTCCTGAC GAGGAAAGCC TTGGACCTCT ACCTGATAAC 
TGGGAAATGG CCTTTACAGA GAATGGAGAA GTCTATTTTA TAGACCATAA CACTAAAACG 
ACATCTTGGT TAGACCCCCG GTGCCTGAAC AAACAGCAGA AGCCTCTGGA AGAATGTGAA 
GATGATGAAG AAGGGGTACA CACCGAGGAA CTGGACAGTG AACTAGGCAT CGGCTCTCCA 
GAATTGCCCG CTGGATGGGA GAAAATTGAA GACCCGGTTT ATGGAGTCTA CTATGTAGAT 
CACATAAACA AGAAAACGCA GTATGAAAAT CCTGTATTGG AGGCCAAACG AAAGAAACAG 
CTTGGACAGC AGCAAAGTGA AGAATGGACA GGGGACCATC CAACCATTGC TCCGCCCCCC 
ATAATGCTTC CTAACAACCT TACTCCAAAA AAGAAGGAAC CAGCAAGAGA AACGGCATCA 
CATGTGAAAC CGTTCTTCAC CAGGAACCCC TCTGAACTGA AAGGCAAGTT CATCCATACG 
AAGCTAAGGA AAAGTAGTCG GGGCTTTGGC TTTACCGTTG TCGGAGGGGA CGAACCAGAT 
GAGTTCTTGC AAATAAAAAG TTTGGTCGTC GACGGTCCGG CCGCGTTGGA CGGCAAAATG 
GAAACGGGTG ATGTTATCGT TAGCGTGAAC GATATCTGCG TCCTTGGCTA CACGCACGCT 
CAAGTTGTGA AGATCTTCCA GTCTATACCC ATTGGGGAAA GTGTGGACCT TGAACTATGC 
CGGGGTTATC CTCTTCCGTT TGACCCAGAT GACCCCAACA CCAGCCTTGT GACATCAGTT 
GCAATATTGG ACAAAGACCC TATCATTGTC AATGGGCAAG AGAATTATGA CTCTCCGTCG 
AGCAACAGCA GTAAAACTAG AAAAGCCAAT AACGTCAAGG ATCCAAGGCC TAGCAGCCCT 
GCTGACATTT CTTCAAATGG TTCCCATGGT TACCCTAATG ATACAGTGTC CTTGGCATCC 
TCTATAGCTA CGCAACCTGA ACTAATTACT GTGCATATAG TAAAAGGACC AATGGGATTT 
GGCTTTACCA TAGCAGACAG TCCTGGCGGT GGGGGCCAGA GAGTGAAGCA GATTGTAGAT 
AGTCCAAGAT GCCGGGGCCT CAAAGAAGGA GATCTTATAG TAGAGGTGAA TAAAAAGAAT 
GTTCAGAACC TTACCCATAA CCAAGTTGTG GATTTACTCA TCGAATGTCC TAAGGGAAGC 
GAAGTTACCC TACTGGTTCA AAGAGGAGGA CTCCCGGTCC CAAAGAAGAG CCCAAAGTCG 
CAACCACTTG AAAGGAAAGA CAGCCAAAAC AGCTCGCAGC ACAGTGTTTC CAGCCAGCAC 
AGTGGGCACA CTGCTTCACC CCATCACGCC GCGCCTGGGC TCCCCGATTA TCCTACCAGC 
GAGGCGCCGG CTGTCGACCA AGCCGACAAC TCCGGGCAGA AAAAGCCTGA TCCTTTCAAA 
ATCTGGGCCC AATCCAGGAG CATGTATGAG AACAGACTTC CAGACTTTCA AGAGCATGAT 
ATATTTTTGT GGCGAAAGGA AACTGGATTT GGGTTTCGAA TTCTCGGTGG AAATGAGCCT 
GGAGAACCAA TCTTCATAGG TCATATTGTG CCACTGGGGT CAGCGGATGC CGACGGGCGG 
TTGCGCTCTG GTGACGAGCT TATCTGTGTG GATGGAACAG CGGTTGTTGG AAAATCTCAT 
CAACTCGTCG TACAGCTTAT GCAACAGGCT GCGAAGCAAG GACACGTCAA TCTAACGATC 
AGACGAAAAA CGATCTATCC GGTTCCAAAG GCAGAGAACG AAGCTCCTGC ATCACCAGCA 
TCCTCGCACC ATAGCAGCAC CCAGCCGGCG TCTCTAACGG AGGAGAAGCG GACGCCCCAA 
GGCAGCCAAA ACTCCTTAAA CACTGTCAGT TCAGGCAGCG GCAGCACCAG CGGCATTGGC 
AGTGGCGGAG GCGGCGGGAG CGGCAACGTC AGCACCGTGG TTCAGCCGTA TGATGTCGAG 
ATCCGACGCG GTGAAAATGA AGGATTTGGA TTTGTCATTG TGTCTTCTGT CAGCCGGCCA 
GAAGCCGGGA CTACTTTTGC CGGCAATGCA TGTGTGGCCA TGCCTCACAA AATAGGTCGG 
ATAATAGAGG GGAGCCCCGC GGATCGCTGC GGCAAGCTGA AAGTGGGGGA CCGGATCTTG 
GCCGTTAACG GGTGCTCCAT CACCAACAAG TCACACTCGG ACATCGTCAA TTTAATAAAG 
GAAGCGGGGA ACACCGTGAC TCTGCGCATC ATTCCCGGCG ATGAAACGTC CAACGCGTCT 
TTATTAACCA ATGCAGAGAA GATTGCCACG ATTACGACCA CGCATACGCC ACAAACGATA 
CCCCAAGAAA CAAAAAATAA TGCAAAACCA AAGCCGGAGC CCCAGTTTGA ATTCAAGAAC 
TCACCAGCCA CACAGCCTGA TCAAGAAATT TATTCAGTGG AACTAGACCG AGGATTAAAA 
GGATTTGGGT TTAGTCTACG AGGAGGTCGA GAATATAACA TGGATCTCTA TGTTTTACGC 
CTTGCGGAGG ATGGGCCTGC AGCCAGATGT GGGAAAATGA GGGTTGGCGA TGAAATCCTG 
GAAATAAACG GAGAGACCAC AAAGAACATG AAGCACGCCC GGGCAATAGA ACTGATTAAA 
AATGGTGGCC GAAGAGTACG CCTGTGTCTG AAACGCGGAG ATGGTTCCGT ACCGGAATAT 
GAACCCGGCG TTGACGGAAA CAGCCCCACA ACCAGTCCAC AAAGTGTATC GAAAATGAAC 
TCGTTACCAT CTGATAGAAA TCACTCATCA CTGGAGTTCA ATTATCCATC CGATCTTCAC 
AAATCATTAC AGCATGGAGA CAAACGAGCT TACGTTAAAG ACCCTAAAGG CAACAGGGAG 
TATGGCAGAC CACATAACGA ACATCACACT TGGAATGGAT CTTCTAAAAA TTATTCTGTA 
CAAAATAACA GAAAAGATCG ATCACCTGAG GGGAGGCGAC CAAGACAGGC CGATCGAACA 
ATGACGAACG GTCAGAAAAG ACAGTCTCCT GAGAAAAGAA ATGAAGGAAC TAGGAGTGCT 
GAGAACACAC TGGAAAGGAG AGAGAAAAAT GAGAGAAGAA AAGAGGTTTC TCCTCAAAAG 
CCCAGGGAAA GGTCGCCGGT GCGCAGAAGG GATGGTTCCC CAACCAGGCG AAGGAAATCC 
TTGGATAGAT TTATGGATCA AAGACGATCC CCTGATAGGA GAAGAGAGAG TTCACCTGAC 
CGAAGAAGAG CAAAGTCAAC GGACAGGAGA CGAGAAAGGT CACCAGAAAG GAGGAGAGAA 
AGGTCTCCTG AGAGAAGAAG AGAGAAGTCC ACTGAGAGAA GAAGGGACAG GTCTCCTGAT 
AAACGAAACA AAGAGCAACG AACCAGCAAC AGAGACAGGG ATGAACACAG TCTGAAATAT 
GATACCCTCA GAAGTGGCAG GCACCAACAA GAACAACGGA GGAGGCAATA CAAGGAATGC 
AGCACCGACC TGAGCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757096.1
CDS	502..4242
Translation	MQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEEGVHTEELDSELGIGSPELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_031901236.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901236.1

ORF Insert Sequence:

ATGCAAAATG CAGGCGTAGT GCATACCGAC CACTATGAGT TTGATGATGA TGCTCCTGAA 
ATGAGCAGTA GCTTTACAGG AGACTCCAGT GAAGCAGACG AGCACACGAT TAACCTTTCC 
CAAGATAAAG TCCCACCGGC GGCTCGGATA CTCCCTACTT TTCCCATCAC GGAAGCTTCT 
CAGAATCTGC CTCATTACCT ACCTCCTGAC GAGGAAAGCC TTGGACCTCT ACCTGATAAC 
TGGGAAATGG CCTTTACAGA GAATGGAGAA GTCTATTTTA TAGACCATAA CACTAAAACG 
ACATCTTGGT TAGACCCCCG GTGCCTGAAC AAACAGCAGA AGCCTCTGGA AGAATGTGAA 
GATGATGAAG AAGGGGTACA CACCGAGGAA CTGGACAGTG AACTAGGCAT CGGCTCTCCA 
GAATTGCCCG CTGGATGGGA GAAAATTGAA GACCCGGTTT ATGGAGTCTA CTATGTAGAT 
CACATAAACA AGAAAACGCA GTATGAAAAT CCTGTATTGG AGGCCAAACG AAAGAAACAG 
CTTGGACAGC AGCAAAGTGA AGAATGGACA GGGGACCATC CAACCATTGC TCCGCCCCCC 
ATAATGCTTC CTAACAACCT TACTCCAAAA AAGAAGGAAC CAGCAAGAGA AACGGCATCA 
CATGTGAAAC CGTTCTTCAC CAGGAACCCC TCTGAACTGA AAGGCAAGTT CATCCATACG 
AAGCTAAGGA AAAGTAGTCG GGGCTTTGGC TTTACCGTTG TCGGAGGGGA CGAACCAGAT 
GAGTTCTTGC AAATAAAAAG TTTGGTCGTC GACGGTCCGG CCGCGTTGGA CGGCAAAATG 
GAAACGGGTG ATGTTATCGT TAGCGTGAAC GATATCTGCG TCCTTGGCTA CACGCACGCT 
CAAGTTGTGA AGATCTTCCA GTCTATACCC ATTGGGGAAA GTGTGGACCT TGAACTATGC 
CGGGGTTATC CTCTTCCGTT TGACCCAGAT GACCCCAACA CCAGCCTTGT GACATCAGTT 
GCAATATTGG ACAAAGACCC TATCATTGTC AATGGGCAAG AGAATTATGA CTCTCCGTCG 
AGCAACAGCA GTAAAACTAG AAAAGCCAAT AACGTCAAGG ATCCAAGGCC TAGCAGCCCT 
GCTGACATTT CTTCAAATGG TTCCCATGGT TACCCTAATG ATACAGTGTC CTTGGCATCC 
TCTATAGCTA CGCAACCTGA ACTAATTACT GTGCATATAG TAAAAGGACC AATGGGATTT 
GGCTTTACCA TAGCAGACAG TCCTGGCGGT GGGGGCCAGA GAGTGAAGCA GATTGTAGAT 
AGTCCAAGAT GCCGGGGCCT CAAAGAAGGA GATCTTATAG TAGAGGTGAA TAAAAAGAAT 
GTTCAGAACC TTACCCATAA CCAAGTTGTG GATTTACTCA TCGAATGTCC TAAGGGAAGC 
GAAGTTACCC TACTGGTTCA AAGAGGAGGA CTCCCGGTCC CAAAGAAGAG CCCAAAGTCG 
CAACCACTTG AAAGGAAAGA CAGCCAAAAC AGCTCGCAGC ACAGTGTTTC CAGCCAGCAC 
AGTGGGCACA CTGCTTCACC CCATCACGCC GCGCCTGGGC TCCCCGATTA TCCTACCAGC 
GAGGCGCCGG CTGTCGACCA AGCCGACAAC TCCGGGCAGA AAAAGCCTGA TCCTTTCAAA 
ATCTGGGCCC AATCCAGGAG CATGTATGAG AACAGACTTC CAGACTTTCA AGAGCATGAT 
ATATTTTTGT GGCGAAAGGA AACTGGATTT GGGTTTCGAA TTCTCGGTGG AAATGAGCCT 
GGAGAACCAA TCTTCATAGG TCATATTGTG CCACTGGGGT CAGCGGATGC CGACGGGCGG 
TTGCGCTCTG GTGACGAGCT TATCTGTGTG GATGGAACAG CGGTTGTTGG AAAATCTCAT 
CAACTCGTCG TACAGCTTAT GCAACAGGCT GCGAAGCAAG GACACGTCAA TCTAACGATC 
AGACGAAAAA CGATCTATCC GGTTCCAAAG GCAGAGAACG AAGCTCCTGC ATCACCAGCA 
TCCTCGCACC ATAGCAGCAC CCAGCCGGCG TCTCTAACGG AGGAGAAGCG GACGCCCCAA 
GGCAGCCAAA ACTCCTTAAA CACTGTCAGT TCAGGCAGCG GCAGCACCAG CGGCATTGGC 
AGTGGCGGAG GCGGCGGGAG CGGCAACGTC AGCACCGTGG TTCAGCCGTA TGATGTCGAG 
ATCCGACGCG GTGAAAATGA AGGATTTGGA TTTGTCATTG TGTCTTCTGT CAGCCGGCCA 
GAAGCCGGGA CTACTTTTGC CGGCAATGCA TGTGTGGCCA TGCCTCACAA AATAGGTCGG 
ATAATAGAGG GGAGCCCCGC GGATCGCTGC GGCAAGCTGA AAGTGGGGGA CCGGATCTTG 
GCCGTTAACG GGTGCTCCAT CACCAACAAG TCACACTCGG ACATCGTCAA TTTAATAAAG 
GAAGCGGGGA ACACCGTGAC TCTGCGCATC ATTCCCGGCG ATGAAACGTC CAACGCGTCT 
TTATTAACCA ATGCAGAGAA GATTGCCACG ATTACGACCA CGCATACGCC ACAAACGATA 
CCCCAAGAAA CAAAAAATAA TGCAAAACCA AAGCCGGAGC CCCAGTTTGA ATTCAAGAAC 
TCACCAGCCA CACAGCCTGA TCAAGAAATT TATTCAGTGG AACTAGACCG AGGATTAAAA 
GGATTTGGGT TTAGTCTACG AGGAGGTCGA GAATATAACA TGGATCTCTA TGTTTTACGC 
CTTGCGGAGG ATGGGCCTGC AGCCAGATGT GGGAAAATGA GGGTTGGCGA TGAAATCCTG 
GAAATAAACG GAGAGACCAC AAAGAACATG AAGCACGCCC GGGCAATAGA ACTGATTAAA 
AATGGTGGCC GAAGAGTACG CCTGTGTCTG AAACGCGGAG ATGGTTCCGT ACCGGAATAT 
GAACCCGGCG TTGACGGAAA CAGCCCCACA ACCAGTCCAC AAAGTGTATC GAAAATGAAC 
TCGTTACCAT CTGATAGAAA TCACTCATCA CTGGAGTTCA ATTATCCATC CGATCTTCAC 
AAATCATTAC AGCATGGAGA CAAACGAGCT TACGTTAAAG ACCCTAAAGG CAACAGGGAG 
TATGGCAGAC CACATAACGA ACATCACACT TGGAATGGAT CTTCTAAAAA TTATTCTGTA 
CAAAATAACA GAAAAGATCG ATCACCTGAG GGGAGGCGAC CAAGACAGGC CGATCGAACA 
ATGACGAACG GTCAGAAAAG ACAGTCTCCT GAGAAAAGAA ATGAAGGAAC TAGGAGTGCT 
GAGAACACAC TGGAAAGGAG AGAGAAAAAT GAGAGAAGAA AAGAGGTTTC TCCTCAAAAG 
CCCAGGGAAA GGTCGCCGGT GCGCAGAAGG GATGGTTCCC CAACCAGGCG AAGGAAATCC 
TTGGATAGAT TTATGGATCA AAGACGATCC CCTGATAGGA GAAGAGAGAG TTCACCTGAC 
CGAAGAAGAG CAAAGTCAAC GGACAGGAGA CGAGAAAGGT CACCAGAAAG GAGGAGAGAA 
AGGTCTCCTG AGAGAAGAAG AGAGAAGTCC ACTGAGAGAA GAAGGGACAG GTCTCCTGAT 
AAACGAAACA AAGAGCAACG AACCAGCAAC AGAGACAGGG ATGAACACAG TCTGAAATAT 
GATACCCTCA GAAGTGGCAG GCACCAACAA GAACAACGGA GGAGGCAATA CAAGGAATGC 
AGCACCGACC TGAGCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901237.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757097.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OXa57633
Clone ID Related Accession (Same CDS sequence)	XM_031901237.1
Accession Version	XM_031901237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATG CAGGCGTAGT GCATACCGAC CACTATGAGT TTGATGATGA TGCTCCTGAA 
ATGAGCAGTA GCTTTACAGG AGACTCCAGT GAAGCAGACG AGCACACGAT TAACCTTTCC 
CAAGATAAAG TCCCACCGGC GGCTCGGATA CTCCCTACTT TTCCCATCAC GGAAGCTTCT 
CAGAATCTGC CTCATTACCT ACCTCCTGAC GAGGAAAGCC TTGGACCTCT ACCTGATAAC 
TGGGAAATGG CCTTTACAGA GAATGGAGAA GTCTATTTTA TAGACCATAA CACTAAAACG 
ACATCTTGGT TAGACCCCCG GTGCCTGAAC AAACAGCAGA AGCCTCTGGA AGAATGTGAA 
GATGATGAAT TGCCCGCTGG ATGGGAGAAA ATTGAAGACC CGGTTTATGG AGTCTACTAT 
GTAGATCACA TAAACAAGAA AACGCAGTAT GAAAATCCTG TATTGGAGGC CAAACGAAAG 
AAACAGCTTG GACAGCAGCA AAGTGAAGAA TGGACAGGGG ACCATCCAAC CATTGCTCCG 
CCCCCCATAA TGCTTCCTAA CAACCTTACT CCAAAAAAGA AGGAACCAGC AAGAGAAACG 
GCATCACATG TGAAACCGTT CTTCACCAGG AACCCCTCTG AACTGAAAGG CAAGTTCATC 
CATACGAAGC TAAGGAAAAG TAGTCGGGGC TTTGGCTTTA CCGTTGTCGG AGGGGACGAA 
CCAGATGAGT TCTTGCAAAT AAAAAGTTTG GTCGTCGACG GTCCGGCCGC GTTGGACGGC 
AAAATGGAAA CGGGTGATGT TATCGTTAGC GTGAACGATA TCTGCGTCCT TGGCTACACG 
CACGCTCAAG TTGTGAAGAT CTTCCAGTCT ATACCCATTG GGGAAAGTGT GGACCTTGAA 
CTATGCCGGG GTTATCCTCT TCCGTTTGAC CCAGATGACC CCAACACCAG CCTTGTGACA 
TCAGTTGCAA TATTGGACAA AGACCCTATC ATTGTCAATG GGCAAGAGAA TTATGACTCT 
CCGTCGAGCA ACAGCAGTAA AACTAGAAAA GCCAATAACG TCAAGGATCC AAGGCCTAGC 
AGCCCTGCTG ACATTTCTTC AAATGGTTCC CATGGTTACC CTAATGATAC AGTGTCCTTG 
GCATCCTCTA TAGCTACGCA ACCTGAACTA ATTACTGTGC ATATAGTAAA AGGACCAATG 
GGATTTGGCT TTACCATAGC AGACAGTCCT GGCGGTGGGG GCCAGAGAGT GAAGCAGATT 
GTAGATAGTC CAAGATGCCG GGGCCTCAAA GAAGGAGATC TTATAGTAGA GGTGAATAAA 
AAGAATGTTC AGAACCTTAC CCATAACCAA GTTGTGGATT TACTCATCGA ATGTCCTAAG 
GGAAGCGAAG TTACCCTACT GGTTCAAAGA GGAGGACTCC CGGTCCCAAA GAAGAGCCCA 
AAGTCGCAAC CACTTGAAAG GAAAGACAGC CAAAACAGCT CGCAGCACAG TGTTTCCAGC 
CAGCACAGTG GGCACACTGC TTCACCCCAT CACGCCGCGC CTGGGCTCCC CGATTATCCT 
ACCAGCGAGG CGCCGGCTGT CGACCAAGCC GACAACTCCG GGCAGAAAAA GCCTGATCCT 
TTCAAAATCT GGGCCCAATC CAGGAGCATG TATGAGAACA GACTTCCAGA CTTTCAAGAG 
CATGATATAT TTTTGTGGCG AAAGGAAACT GGATTTGGGT TTCGAATTCT CGGTGGAAAT 
GAGCCTGGAG AACCAATCTT CATAGGTCAT ATTGTGCCAC TGGGGTCAGC GGATGCCGAC 
GGGCGGTTGC GCTCTGGTGA CGAGCTTATC TGTGTGGATG GAACAGCGGT TGTTGGAAAA 
TCTCATCAAC TCGTCGTACA GCTTATGCAA CAGGCTGCGA AGCAAGGACA CGTCAATCTA 
ACGATCAGAC GAAAAACGAT CTATCCGGTT CCAAAGGCAG AGAACGAAGC TCCTGCATCA 
CCAGCATCCT CGCACCATAG CAGCACCCAG CCGGCGTCTC TAACGGAGGA GAAGCGGACG 
CCCCAAGGCA GCCAAAACTC CTTAAACACT GTCAGTTCAG GCAGCGGCAG CACCAGCGGC 
ATTGGCAGTG GCGGAGGCGG CGGGAGCGGC AACGTCAGCA CCGTGGTTCA GCCGTATGAT 
GTCGAGATCC GACGCGGTGA AAATGAAGGA TTTGGATTTG TCATTGTGTC TTCTGTCAGC 
CGGCCAGAAG CCGGGACTAC TTTTGCCGGC AATGCATGTG TGGCCATGCC TCACAAAATA 
GGTCGGATAA TAGAGGGGAG CCCCGCGGAT CGCTGCGGCA AGCTGAAAGT GGGGGACCGG 
ATCTTGGCCG TTAACGGGTG CTCCATCACC AACAAGTCAC ACTCGGACAT CGTCAATTTA 
ATAAAGGAAG CGGGGAACAC CGTGACTCTG CGCATCATTC CCGGCGATGA AACGTCCAAC 
GCGTCTTTAT TAACCAATGC AGAGAAGATT GCCACGATTA CGACCACGCA TACGCCACAA 
ACGATACCCC AAGAAACAAA AAATAATGCA AAACCAAAGC CGGAGCCCCA GTTTGAATTC 
AAGAACTCAC CAGCCACACA GCCTGATCAA GAAATTTATT CAGTGGAACT AGACCGAGGA 
TTAAAAGGAT TTGGGTTTAG TCTACGAGGA GGTCGAGAAT ATAACATGGA TCTCTATGTT 
TTACGCCTTG CGGAGGATGG GCCTGCAGCC AGATGTGGGA AAATGAGGGT TGGCGATGAA 
ATCCTGGAAA TAAACGGAGA GACCACAAAG AACATGAAGC ACGCCCGGGC AATAGAACTG 
ATTAAAAATG GTGGCCGAAG AGTACGCCTG TGTCTGAAAC GCGGAGATGG TTCCGTACCG 
GAATATGAAC CCGGCGTTGA CGGAAACAGC CCCACAACCA GTCCACAAAG TGTATCGAAA 
ATGAACTCGT TACCATCTGA TAGAAATCAC TCATCACTGG AGTTCAATTA TCCATCCGAT 
CTTCACAAAT CATTACAGCA TGGAGACAAA CGAGCTTACG TTAAAGACCC TAAAGGCAAC 
AGGGAGTATG GCAGACCACA TAACGAACAT CACACTTGGA ATGGATCTTC TAAAAATTAT 
TCTGTACAAA ATAACAGAAA AGATCGATCA CCTGAGGGGA GGCGACCAAG ACAGGCCGAT 
CGAACAATGA CGAACGGTCA GAAAAGACAG TCTCCTGAGA AAAGAAATGA AGGAACTAGG 
AGTGCTGAGA ACACACTGGA AAGGAGAGAG AAAAATGAGA GAAGAAAAGA GGTTTCTCCT 
CAAAAGCCCA GGGAAAGGTC GCCGGTGCGC AGAAGGGATG GTTCCCCAAC CAGGCGAAGG 
AAATCCTTGG ATAGATTTAT GGATCAAAGA CGATCCCCTG ATAGGAGAAG AGAGAGTTCA 
CCTGACCGAA GAAGAGCAAA GTCAACGGAC AGGAGACGAG AAAGGTCACC AGAAAGGAGG 
AGAGAAAGGT CTCCTGAGAG AAGAAGAGAG AAGTCCACTG AGAGAAGAAG GGACAGGTCT 
CCTGATAAAC GAAACAAAGA GCAACGAACC AGCAACAGAG ACAGGGATGA ACACAGTCTG 
AAATATGATA CCCTCAGAAG TGGCAGGCAC CAACAAGAAC AACGGAGGAG GCAATACAAG 
GAATGCAGCA CCGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757097.1
CDS	501..4187
Translation	MQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYEPGVDGNSPTTSPQSVSKMNSLPSDRNHSSLEFNYPSDLHKSLQHGDKRAYVKDPKGNREYGRPHNEHHTWNGSSKNYSVQNNRKDRSPEGRRPRQADRTMTNGQKRQSPEKRNEGTRSAENTLERREKNERRKEVSPQKPRERSPVRRRDGSPTRRRKSLDRFMDQRRSPDRRRESSPDRRRAKSTDRRRERSPERRRERSPERRREKSTERRRDRSPDKRNKEQRTSNRDRDEHSLKYDTLRSGRHQQEQRRRQYKECSTDLSI

Target ORF information:

RefSeq Version	XM_031901237.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901237.1

ORF Insert Sequence:

ATGCAAAATG CAGGCGTAGT GCATACCGAC CACTATGAGT TTGATGATGA TGCTCCTGAA 
ATGAGCAGTA GCTTTACAGG AGACTCCAGT GAAGCAGACG AGCACACGAT TAACCTTTCC 
CAAGATAAAG TCCCACCGGC GGCTCGGATA CTCCCTACTT TTCCCATCAC GGAAGCTTCT 
CAGAATCTGC CTCATTACCT ACCTCCTGAC GAGGAAAGCC TTGGACCTCT ACCTGATAAC 
TGGGAAATGG CCTTTACAGA GAATGGAGAA GTCTATTTTA TAGACCATAA CACTAAAACG 
ACATCTTGGT TAGACCCCCG GTGCCTGAAC AAACAGCAGA AGCCTCTGGA AGAATGTGAA 
GATGATGAAT TGCCCGCTGG ATGGGAGAAA ATTGAAGACC CGGTTTATGG AGTCTACTAT 
GTAGATCACA TAAACAAGAA AACGCAGTAT GAAAATCCTG TATTGGAGGC CAAACGAAAG 
AAACAGCTTG GACAGCAGCA AAGTGAAGAA TGGACAGGGG ACCATCCAAC CATTGCTCCG 
CCCCCCATAA TGCTTCCTAA CAACCTTACT CCAAAAAAGA AGGAACCAGC AAGAGAAACG 
GCATCACATG TGAAACCGTT CTTCACCAGG AACCCCTCTG AACTGAAAGG CAAGTTCATC 
CATACGAAGC TAAGGAAAAG TAGTCGGGGC TTTGGCTTTA CCGTTGTCGG AGGGGACGAA 
CCAGATGAGT TCTTGCAAAT AAAAAGTTTG GTCGTCGACG GTCCGGCCGC GTTGGACGGC 
AAAATGGAAA CGGGTGATGT TATCGTTAGC GTGAACGATA TCTGCGTCCT TGGCTACACG 
CACGCTCAAG TTGTGAAGAT CTTCCAGTCT ATACCCATTG GGGAAAGTGT GGACCTTGAA 
CTATGCCGGG GTTATCCTCT TCCGTTTGAC CCAGATGACC CCAACACCAG CCTTGTGACA 
TCAGTTGCAA TATTGGACAA AGACCCTATC ATTGTCAATG GGCAAGAGAA TTATGACTCT 
CCGTCGAGCA ACAGCAGTAA AACTAGAAAA GCCAATAACG TCAAGGATCC AAGGCCTAGC 
AGCCCTGCTG ACATTTCTTC AAATGGTTCC CATGGTTACC CTAATGATAC AGTGTCCTTG 
GCATCCTCTA TAGCTACGCA ACCTGAACTA ATTACTGTGC ATATAGTAAA AGGACCAATG 
GGATTTGGCT TTACCATAGC AGACAGTCCT GGCGGTGGGG GCCAGAGAGT GAAGCAGATT 
GTAGATAGTC CAAGATGCCG GGGCCTCAAA GAAGGAGATC TTATAGTAGA GGTGAATAAA 
AAGAATGTTC AGAACCTTAC CCATAACCAA GTTGTGGATT TACTCATCGA ATGTCCTAAG 
GGAAGCGAAG TTACCCTACT GGTTCAAAGA GGAGGACTCC CGGTCCCAAA GAAGAGCCCA 
AAGTCGCAAC CACTTGAAAG GAAAGACAGC CAAAACAGCT CGCAGCACAG TGTTTCCAGC 
CAGCACAGTG GGCACACTGC TTCACCCCAT CACGCCGCGC CTGGGCTCCC CGATTATCCT 
ACCAGCGAGG CGCCGGCTGT CGACCAAGCC GACAACTCCG GGCAGAAAAA GCCTGATCCT 
TTCAAAATCT GGGCCCAATC CAGGAGCATG TATGAGAACA GACTTCCAGA CTTTCAAGAG 
CATGATATAT TTTTGTGGCG AAAGGAAACT GGATTTGGGT TTCGAATTCT CGGTGGAAAT 
GAGCCTGGAG AACCAATCTT CATAGGTCAT ATTGTGCCAC TGGGGTCAGC GGATGCCGAC 
GGGCGGTTGC GCTCTGGTGA CGAGCTTATC TGTGTGGATG GAACAGCGGT TGTTGGAAAA 
TCTCATCAAC TCGTCGTACA GCTTATGCAA CAGGCTGCGA AGCAAGGACA CGTCAATCTA 
ACGATCAGAC GAAAAACGAT CTATCCGGTT CCAAAGGCAG AGAACGAAGC TCCTGCATCA 
CCAGCATCCT CGCACCATAG CAGCACCCAG CCGGCGTCTC TAACGGAGGA GAAGCGGACG 
CCCCAAGGCA GCCAAAACTC CTTAAACACT GTCAGTTCAG GCAGCGGCAG CACCAGCGGC 
ATTGGCAGTG GCGGAGGCGG CGGGAGCGGC AACGTCAGCA CCGTGGTTCA GCCGTATGAT 
GTCGAGATCC GACGCGGTGA AAATGAAGGA TTTGGATTTG TCATTGTGTC TTCTGTCAGC 
CGGCCAGAAG CCGGGACTAC TTTTGCCGGC AATGCATGTG TGGCCATGCC TCACAAAATA 
GGTCGGATAA TAGAGGGGAG CCCCGCGGAT CGCTGCGGCA AGCTGAAAGT GGGGGACCGG 
ATCTTGGCCG TTAACGGGTG CTCCATCACC AACAAGTCAC ACTCGGACAT CGTCAATTTA 
ATAAAGGAAG CGGGGAACAC CGTGACTCTG CGCATCATTC CCGGCGATGA AACGTCCAAC 
GCGTCTTTAT TAACCAATGC AGAGAAGATT GCCACGATTA CGACCACGCA TACGCCACAA 
ACGATACCCC AAGAAACAAA AAATAATGCA AAACCAAAGC CGGAGCCCCA GTTTGAATTC 
AAGAACTCAC CAGCCACACA GCCTGATCAA GAAATTTATT CAGTGGAACT AGACCGAGGA 
TTAAAAGGAT TTGGGTTTAG TCTACGAGGA GGTCGAGAAT ATAACATGGA TCTCTATGTT 
TTACGCCTTG CGGAGGATGG GCCTGCAGCC AGATGTGGGA AAATGAGGGT TGGCGATGAA 
ATCCTGGAAA TAAACGGAGA GACCACAAAG AACATGAAGC ACGCCCGGGC AATAGAACTG 
ATTAAAAATG GTGGCCGAAG AGTACGCCTG TGTCTGAAAC GCGGAGATGG TTCCGTACCG 
GAATATGAAC CCGGCGTTGA CGGAAACAGC CCCACAACCA GTCCACAAAG TGTATCGAAA 
ATGAACTCGT TACCATCTGA TAGAAATCAC TCATCACTGG AGTTCAATTA TCCATCCGAT 
CTTCACAAAT CATTACAGCA TGGAGACAAA CGAGCTTACG TTAAAGACCC TAAAGGCAAC 
AGGGAGTATG GCAGACCACA TAACGAACAT CACACTTGGA ATGGATCTTC TAAAAATTAT 
TCTGTACAAA ATAACAGAAA AGATCGATCA CCTGAGGGGA GGCGACCAAG ACAGGCCGAT 
CGAACAATGA CGAACGGTCA GAAAAGACAG TCTCCTGAGA AAAGAAATGA AGGAACTAGG 
AGTGCTGAGA ACACACTGGA AAGGAGAGAG AAAAATGAGA GAAGAAAAGA GGTTTCTCCT 
CAAAAGCCCA GGGAAAGGTC GCCGGTGCGC AGAAGGGATG GTTCCCCAAC CAGGCGAAGG 
AAATCCTTGG ATAGATTTAT GGATCAAAGA CGATCCCCTG ATAGGAGAAG AGAGAGTTCA 
CCTGACCGAA GAAGAGCAAA GTCAACGGAC AGGAGACGAG AAAGGTCACC AGAAAGGAGG 
AGAGAAAGGT CTCCTGAGAG AAGAAGAGAG AAGTCCACTG AGAGAAGAAG GGACAGGTCT 
CCTGATAAAC GAAACAAAGA GCAACGAACC AGCAACAGAG ACAGGGATGA ACACAGTCTG 
AAATATGATA CCCTCAGAAG TGGCAGGCAC CAACAAGAAC AACGGAGGAG GCAATACAAG 
GAATGCAGCA CCGACCTGAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901238.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757098.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OXa57634
Clone ID Related Accession (Same CDS sequence)	XM_031901238.1
Accession Version	XM_031901238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGGCATCGGC 
TCTCCAGAAT TGCCCGCTGG ATGGGAGAAA ATTGAAGACC CGGTTTATGG AGTCTACTAT 
GTAGATCACA TAAACAAGAA AACGCAGTAT GAAAATCCTG TATTGGAGGC CAAACGAAAG 
AAACAGCTTG GACAGCAGCA AAGTGAAGAA TGGACAGGGG ACCATCCAAC CATTGCTCCG 
CCCCCCATAA TGCTTCCTAA CAACCTTACT CCAAAAAAGA AGGAACCAGC AAGAGAAACG 
GCATCACATG TGAAACCGTT CTTCACCAGG AACCCCTCTG AACTGAAAGG CAAGTTCATC 
CATACGAAGC TAAGGAAAAG TAGTCGGGGC TTTGGCTTTA CCGTTGTCGG AGGGGACGAA 
CCAGATGAGT TCTTGCAAAT AAAAAGTTTG GTCGTCGACG GTCCGGCCGC GTTGGACGGC 
AAAATGGAAA CGGGTGATGT TATCGTTAGC GTGAACGATA TCTGCGTCCT TGGCTACACG 
CACGCTCAAG TTGTGAAGAT CTTCCAGTCT ATACCCATTG GGGAAAGTGT GGACCTTGAA 
CTATGCCGGG GTTATCCTCT TCCGTTTGAC CCAGATGACC CCAACACCAG CCTTGTGACA 
TCAGTTGCAA TATTGGACAA AGACCCTATC ATTGTCAATG GGCAAGAGAA TTATGACTCT 
CCGTCGAGCA ACAGCAGTAA AACTAGAAAA GCCAATAACG TCAAGGATCC AAGGCCTAGC 
AGCCCTGCTG ACATTTCTTC AAATGGTTCC CATGGTTACC CTAATGATAC AGTGTCCTTG 
GCATCCTCTA TAGCTACGCA ACCTGAACTA ATTACTGTGC ATATAGTAAA AGGACCAATG 
GGATTTGGCT TTACCATAGC AGACAGTCCT GGCGGTGGGG GCCAGAGAGT GAAGCAGATT 
GTAGATAGTC CAAGATGCCG GGGCCTCAAA GAAGGAGATC TTATAGTAGA GGTGAATAAA 
AAGAATGTTC AGAACCTTAC CCATAACCAA GTTGTGGATT TACTCATCGA ATGTCCTAAG 
GGAAGCGAAG TTACCCTACT GGTTCAAAGA GGAGGACTCC CGGTCCCAAA GAAGAGCCCA 
AAGTCGCAAC CACTTGAAAG GAAAGACAGC CAAAACAGCT CGCAGCACAG TGTTTCCAGC 
CAGCACAGTG GGCACACTGC TTCACCCCAT CACGCCGCGC CTGGGCTCCC CGATTATCCT 
ACCAGCGAGG CGCCGGCTGT CGACCAAGCC GACAACTCCG GGCAGAAAAA GCCTGATCCT 
TTCAAAATCT GGGCCCAATC CAGGAGCATG TATGAGAACA GACTTCCAGA CTTTCAAGAG 
CATGATATAT TTTTGTGGCG AAAGGAAACT GGATTTGGGT TTCGAATTCT CGGTGGAAAT 
GAGCCTGGAG AACCAATCTT CATAGGTCAT ATTGTGCCAC TGGGGTCAGC GGATGCCGAC 
GGGCGGTTGC GCTCTGGTGA CGAGCTTATC TGTGTGGATG GAACAGCGGT TGTTGGAAAA 
TCTCATCAAC TCGTCGTACA GCTTATGCAA CAGGCTGCGA AGCAAGGACA CGTCAATCTA 
ACGATCAGAC GAAAAACGAT CTATCCGGTT CCAAAGGCAG AGAACGAAGC TCCTGCATCA 
CCAGCATCCT CGCACCATAG CAGCACCCAG CCGGCGTCTC TAACGGAGGA GAAGCGGACG 
CCCCAAGGCA GCCAAAACTC CTTAAACACT GTCAGTTCAG GCAGCGGCAG CACCAGCGGC 
ATTGGCAGTG GCGGAGGCGG CGGGAGCGGC AACGTCAGCA CCGTGGTTCA GCCGTATGAT 
GTCGAGATCC GACGCGGTGA AAATGAAGGA TTTGGATTTG TCATTGTGTC TTCTGTCAGC 
CGGCCAGAAG CCGGGACTAC TTTTGCCGGC AATGCATGTG TGGCCATGCC TCACAAAATA 
GGTCGGATAA TAGAGGGGAG CCCCGCGGAT CGCTGCGGCA AGCTGAAAGT GGGGGACCGG 
ATCTTGGCCG TTAACGGGTG CTCCATCACC AACAAGTCAC ACTCGGACAT CGTCAATTTA 
ATAAAGGAAG CGGGGAACAC CGTGACTCTG CGCATCATTC CCGGCGATGA AACGTCCAAC 
GCGTCTTTAT TAACCAATGC AGAGAAGATT GCCACGATTA CGACCACGCA TACGCCACAA 
ACGATACCCC AAGAAACAAA AAATAATGCA AAACCAAAGC CGGAGCCCCA GTTTGAATTC 
AAGAACTCAC CAGCCACACA GCCTGATCAA GAAATTTATT CAGTGGAACT AGACCGAGGA 
TTAAAAGGAT TTGGGTTTAG TCTACGAGGA GGTCGAGAAT ATAACATGGA TCTCTATGTT 
TTACGCCTTG CGGAGGATGG GCCTGCAGCC AGATGTGGGA AAATGAGGGT TGGCGATGAA 
ATCCTGGAAA TAAACGGAGA GACCACAAAG AACATGAAGC ACGCCCGGGC AATAGAACTG 
ATTAAAAATG GTGGCCGAAG AGTACGCCTG TGTCTGAAAC GCGGAGATGG TTCCGTACCG 
GAATATGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757098.1
CDS	278..3889
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEEGVHTEELDSELGIGSPELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYE

Target ORF information:

RefSeq Version	XM_031901238.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901238.1

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGGCATCGGC 
TCTCCAGAAT TGCCCGCTGG ATGGGAGAAA ATTGAAGACC CGGTTTATGG AGTCTACTAT 
GTAGATCACA TAAACAAGAA AACGCAGTAT GAAAATCCTG TATTGGAGGC CAAACGAAAG 
AAACAGCTTG GACAGCAGCA AAGTGAAGAA TGGACAGGGG ACCATCCAAC CATTGCTCCG 
CCCCCCATAA TGCTTCCTAA CAACCTTACT CCAAAAAAGA AGGAACCAGC AAGAGAAACG 
GCATCACATG TGAAACCGTT CTTCACCAGG AACCCCTCTG AACTGAAAGG CAAGTTCATC 
CATACGAAGC TAAGGAAAAG TAGTCGGGGC TTTGGCTTTA CCGTTGTCGG AGGGGACGAA 
CCAGATGAGT TCTTGCAAAT AAAAAGTTTG GTCGTCGACG GTCCGGCCGC GTTGGACGGC 
AAAATGGAAA CGGGTGATGT TATCGTTAGC GTGAACGATA TCTGCGTCCT TGGCTACACG 
CACGCTCAAG TTGTGAAGAT CTTCCAGTCT ATACCCATTG GGGAAAGTGT GGACCTTGAA 
CTATGCCGGG GTTATCCTCT TCCGTTTGAC CCAGATGACC CCAACACCAG CCTTGTGACA 
TCAGTTGCAA TATTGGACAA AGACCCTATC ATTGTCAATG GGCAAGAGAA TTATGACTCT 
CCGTCGAGCA ACAGCAGTAA AACTAGAAAA GCCAATAACG TCAAGGATCC AAGGCCTAGC 
AGCCCTGCTG ACATTTCTTC AAATGGTTCC CATGGTTACC CTAATGATAC AGTGTCCTTG 
GCATCCTCTA TAGCTACGCA ACCTGAACTA ATTACTGTGC ATATAGTAAA AGGACCAATG 
GGATTTGGCT TTACCATAGC AGACAGTCCT GGCGGTGGGG GCCAGAGAGT GAAGCAGATT 
GTAGATAGTC CAAGATGCCG GGGCCTCAAA GAAGGAGATC TTATAGTAGA GGTGAATAAA 
AAGAATGTTC AGAACCTTAC CCATAACCAA GTTGTGGATT TACTCATCGA ATGTCCTAAG 
GGAAGCGAAG TTACCCTACT GGTTCAAAGA GGAGGACTCC CGGTCCCAAA GAAGAGCCCA 
AAGTCGCAAC CACTTGAAAG GAAAGACAGC CAAAACAGCT CGCAGCACAG TGTTTCCAGC 
CAGCACAGTG GGCACACTGC TTCACCCCAT CACGCCGCGC CTGGGCTCCC CGATTATCCT 
ACCAGCGAGG CGCCGGCTGT CGACCAAGCC GACAACTCCG GGCAGAAAAA GCCTGATCCT 
TTCAAAATCT GGGCCCAATC CAGGAGCATG TATGAGAACA GACTTCCAGA CTTTCAAGAG 
CATGATATAT TTTTGTGGCG AAAGGAAACT GGATTTGGGT TTCGAATTCT CGGTGGAAAT 
GAGCCTGGAG AACCAATCTT CATAGGTCAT ATTGTGCCAC TGGGGTCAGC GGATGCCGAC 
GGGCGGTTGC GCTCTGGTGA CGAGCTTATC TGTGTGGATG GAACAGCGGT TGTTGGAAAA 
TCTCATCAAC TCGTCGTACA GCTTATGCAA CAGGCTGCGA AGCAAGGACA CGTCAATCTA 
ACGATCAGAC GAAAAACGAT CTATCCGGTT CCAAAGGCAG AGAACGAAGC TCCTGCATCA 
CCAGCATCCT CGCACCATAG CAGCACCCAG CCGGCGTCTC TAACGGAGGA GAAGCGGACG 
CCCCAAGGCA GCCAAAACTC CTTAAACACT GTCAGTTCAG GCAGCGGCAG CACCAGCGGC 
ATTGGCAGTG GCGGAGGCGG CGGGAGCGGC AACGTCAGCA CCGTGGTTCA GCCGTATGAT 
GTCGAGATCC GACGCGGTGA AAATGAAGGA TTTGGATTTG TCATTGTGTC TTCTGTCAGC 
CGGCCAGAAG CCGGGACTAC TTTTGCCGGC AATGCATGTG TGGCCATGCC TCACAAAATA 
GGTCGGATAA TAGAGGGGAG CCCCGCGGAT CGCTGCGGCA AGCTGAAAGT GGGGGACCGG 
ATCTTGGCCG TTAACGGGTG CTCCATCACC AACAAGTCAC ACTCGGACAT CGTCAATTTA 
ATAAAGGAAG CGGGGAACAC CGTGACTCTG CGCATCATTC CCGGCGATGA AACGTCCAAC 
GCGTCTTTAT TAACCAATGC AGAGAAGATT GCCACGATTA CGACCACGCA TACGCCACAA 
ACGATACCCC AAGAAACAAA AAATAATGCA AAACCAAAGC CGGAGCCCCA GTTTGAATTC 
AAGAACTCAC CAGCCACACA GCCTGATCAA GAAATTTATT CAGTGGAACT AGACCGAGGA 
TTAAAAGGAT TTGGGTTTAG TCTACGAGGA GGTCGAGAAT ATAACATGGA TCTCTATGTT 
TTACGCCTTG CGGAGGATGG GCCTGCAGCC AGATGTGGGA AAATGAGGGT TGGCGATGAA 
ATCCTGGAAA TAAACGGAGA GACCACAAAG AACATGAAGC ACGCCCGGGC AATAGAACTG 
ATTAAAAATG GTGGCCGAAG AGTACGCCTG TGTCTGAAAC GCGGAGATGG TTCCGTACCG 
GAATATGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901240.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757100.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X13, mRNA.

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CloneID	OXa57635
Clone ID Related Accession (Same CDS sequence)	XM_031901240.1
Accession Version	XM_031901240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAATTGCC CGCTGGATGG GAGAAAATTG AAGACCCGGT TTATGGAGTC 
TACTATGTAG ATCACATAAA CAAGAAAACG CAGTATGAAA ATCCTGTATT GGAGGCCAAA 
CGAAAGAAAC AGCTTGGACA GCAGCAAAGT GAAGAATGGA CAGGGGACCA TCCAACCATT 
GCTCCGCCCC CCATAATGCT TCCTAACAAC CTTACTCCAA AAAAGAAGGA ACCAGCAAGA 
GAAACGGCAT CACATGTGAA ACCGTTCTTC ACCAGGAACC CCTCTGAACT GAAAGGCAAG 
TTCATCCATA CGAAGCTAAG GAAAAGTAGT CGGGGCTTTG GCTTTACCGT TGTCGGAGGG 
GACGAACCAG ATGAGTTCTT GCAAATAAAA AGTTTGGTCG TCGACGGTCC GGCCGCGTTG 
GACGGCAAAA TGGAAACGGG TGATGTTATC GTTAGCGTGA ACGATATCTG CGTCCTTGGC 
TACACGCACG CTCAAGTTGT GAAGATCTTC CAGTCTATAC CCATTGGGGA AAGTGTGGAC 
CTTGAACTAT GCCGGGGTTA TCCTCTTCCG TTTGACCCAG ATGACCCCAA CACCAGCCTT 
GTGACATCAG TTGCAATATT GGACAAAGAC CCTATCATTG TCAATGGGCA AGAGAATTAT 
GACTCTCCGT CGAGCAACAG CAGTAAAACT AGAAAAGCCA ATAACGTCAA GGATCCAAGG 
CCTAGCAGCC CTGCTGACAT TTCTTCAAAT GGTTCCCATG GTTACCCTAA TGATACAGTG 
TCCTTGGCAT CCTCTATAGC TACGCAACCT GAACTAATTA CTGTGCATAT AGTAAAAGGA 
CCAATGGGAT TTGGCTTTAC CATAGCAGAC AGTCCTGGCG GTGGGGGCCA GAGAGTGAAG 
CAGATTGTAG ATAGTCCAAG ATGCCGGGGC CTCAAAGAAG GAGATCTTAT AGTAGAGGTG 
AATAAAAAGA ATGTTCAGAA CCTTACCCAT AACCAAGTTG TGGATTTACT CATCGAATGT 
CCTAAGGGAA GCGAAGTTAC CCTACTGGTT CAAAGAGGAG GACTCCCGGT CCCAAAGAAG 
AGCCCAAAGT CGCAACCACT TGAAAGGAAA GACAGCCAAA ACAGCTCGCA GCACAGTGTT 
TCCAGCCAGC ACAGTGGGCA CACTGCTTCA CCCCATCACG CCGCGCCTGG GCTCCCCGAT 
TATCCTACCA GCGAGGCGCC GGCTGTCGAC CAAGCCGACA ACTCCGGGCA GAAAAAGCCT 
GATCCTTTCA AAATCTGGGC CCAATCCAGG AGCATGTATG AGAACAGACT TCCAGACTTT 
CAAGAGCATG ATATATTTTT GTGGCGAAAG GAAACTGGAT TTGGGTTTCG AATTCTCGGT 
GGAAATGAGC CTGGAGAACC AATCTTCATA GGTCATATTG TGCCACTGGG GTCAGCGGAT 
GCCGACGGGC GGTTGCGCTC TGGTGACGAG CTTATCTGTG TGGATGGAAC AGCGGTTGTT 
GGAAAATCTC ATCAACTCGT CGTACAGCTT ATGCAACAGG CTGCGAAGCA AGGACACGTC 
AATCTAACGA TCAGACGAAA AACGATCTAT CCGGTTCCAA AGGCAGAGAA CGAAGCTCCT 
GCATCACCAG CATCCTCGCA CCATAGCAGC ACCCAGCCGG CGTCTCTAAC GGAGGAGAAG 
CGGACGCCCC AAGGCAGCCA AAACTCCTTA AACACTGTCA GTTCAGGCAG CGGCAGCACC 
AGCGGCATTG GCAGTGGCGG AGGCGGCGGG AGCGGCAACG TCAGCACCGT GGTTCAGCCG 
TATGATGTCG AGATCCGACG CGGTGAAAAT GAAGGATTTG GATTTGTCAT TGTGTCTTCT 
GTCAGCCGGC CAGAAGCCGG GACTACTTTT GCCGGCAATG CATGTGTGGC CATGCCTCAC 
AAAATAGGTC GGATAATAGA GGGGAGCCCC GCGGATCGCT GCGGCAAGCT GAAAGTGGGG 
GACCGGATCT TGGCCGTTAA CGGGTGCTCC ATCACCAACA AGTCACACTC GGACATCGTC 
AATTTAATAA AGGAAGCGGG GAACACCGTG ACTCTGCGCA TCATTCCCGG CGATGAAACG 
TCCAACGCGT CTTTATTAAC CAATGCAGAG AAGATTGCCA CGATTACGAC CACGCATACG 
CCACAAACGA TACCCCAAGA AACAAAAAAT AATGCAAAAC CAAAGCCGGA GCCCCAGTTT 
GAATTCAAGA ACTCACCAGC CACACAGCCT GATCAAGAAA TTTATTCAGT GGAACTAGAC 
CGAGGATTAA AAGGATTTGG GTTTAGTCTA CGAGGAGGTC GAGAATATAA CATGGATCTC 
TATGTTTTAC GCCTTGCGGA GGATGGGCCT GCAGCCAGAT GTGGGAAAAT GAGGGTTGGC 
GATGAAATCC TGGAAATAAA CGGAGAGACC ACAAAGAACA TGAAGCACGC CCGGGCAATA 
GAACTGATTA AAAATGGTGG CCGAAGAGTA CGCCTGTGTC TGAAACGCGG AGATGGTTCC 
GTACCGGAAT ATGGTGGGTC AAACTTTGAA AACATTCCTT TGCTTCCTGC CGTCACTCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757100.1
CDS	277..3879
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYGGSNFENIPLLPAVTP

Target ORF information:

RefSeq Version	XM_031901240.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901240.1

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAATTGCC CGCTGGATGG GAGAAAATTG AAGACCCGGT TTATGGAGTC 
TACTATGTAG ATCACATAAA CAAGAAAACG CAGTATGAAA ATCCTGTATT GGAGGCCAAA 
CGAAAGAAAC AGCTTGGACA GCAGCAAAGT GAAGAATGGA CAGGGGACCA TCCAACCATT 
GCTCCGCCCC CCATAATGCT TCCTAACAAC CTTACTCCAA AAAAGAAGGA ACCAGCAAGA 
GAAACGGCAT CACATGTGAA ACCGTTCTTC ACCAGGAACC CCTCTGAACT GAAAGGCAAG 
TTCATCCATA CGAAGCTAAG GAAAAGTAGT CGGGGCTTTG GCTTTACCGT TGTCGGAGGG 
GACGAACCAG ATGAGTTCTT GCAAATAAAA AGTTTGGTCG TCGACGGTCC GGCCGCGTTG 
GACGGCAAAA TGGAAACGGG TGATGTTATC GTTAGCGTGA ACGATATCTG CGTCCTTGGC 
TACACGCACG CTCAAGTTGT GAAGATCTTC CAGTCTATAC CCATTGGGGA AAGTGTGGAC 
CTTGAACTAT GCCGGGGTTA TCCTCTTCCG TTTGACCCAG ATGACCCCAA CACCAGCCTT 
GTGACATCAG TTGCAATATT GGACAAAGAC CCTATCATTG TCAATGGGCA AGAGAATTAT 
GACTCTCCGT CGAGCAACAG CAGTAAAACT AGAAAAGCCA ATAACGTCAA GGATCCAAGG 
CCTAGCAGCC CTGCTGACAT TTCTTCAAAT GGTTCCCATG GTTACCCTAA TGATACAGTG 
TCCTTGGCAT CCTCTATAGC TACGCAACCT GAACTAATTA CTGTGCATAT AGTAAAAGGA 
CCAATGGGAT TTGGCTTTAC CATAGCAGAC AGTCCTGGCG GTGGGGGCCA GAGAGTGAAG 
CAGATTGTAG ATAGTCCAAG ATGCCGGGGC CTCAAAGAAG GAGATCTTAT AGTAGAGGTG 
AATAAAAAGA ATGTTCAGAA CCTTACCCAT AACCAAGTTG TGGATTTACT CATCGAATGT 
CCTAAGGGAA GCGAAGTTAC CCTACTGGTT CAAAGAGGAG GACTCCCGGT CCCAAAGAAG 
AGCCCAAAGT CGCAACCACT TGAAAGGAAA GACAGCCAAA ACAGCTCGCA GCACAGTGTT 
TCCAGCCAGC ACAGTGGGCA CACTGCTTCA CCCCATCACG CCGCGCCTGG GCTCCCCGAT 
TATCCTACCA GCGAGGCGCC GGCTGTCGAC CAAGCCGACA ACTCCGGGCA GAAAAAGCCT 
GATCCTTTCA AAATCTGGGC CCAATCCAGG AGCATGTATG AGAACAGACT TCCAGACTTT 
CAAGAGCATG ATATATTTTT GTGGCGAAAG GAAACTGGAT TTGGGTTTCG AATTCTCGGT 
GGAAATGAGC CTGGAGAACC AATCTTCATA GGTCATATTG TGCCACTGGG GTCAGCGGAT 
GCCGACGGGC GGTTGCGCTC TGGTGACGAG CTTATCTGTG TGGATGGAAC AGCGGTTGTT 
GGAAAATCTC ATCAACTCGT CGTACAGCTT ATGCAACAGG CTGCGAAGCA AGGACACGTC 
AATCTAACGA TCAGACGAAA AACGATCTAT CCGGTTCCAA AGGCAGAGAA CGAAGCTCCT 
GCATCACCAG CATCCTCGCA CCATAGCAGC ACCCAGCCGG CGTCTCTAAC GGAGGAGAAG 
CGGACGCCCC AAGGCAGCCA AAACTCCTTA AACACTGTCA GTTCAGGCAG CGGCAGCACC 
AGCGGCATTG GCAGTGGCGG AGGCGGCGGG AGCGGCAACG TCAGCACCGT GGTTCAGCCG 
TATGATGTCG AGATCCGACG CGGTGAAAAT GAAGGATTTG GATTTGTCAT TGTGTCTTCT 
GTCAGCCGGC CAGAAGCCGG GACTACTTTT GCCGGCAATG CATGTGTGGC CATGCCTCAC 
AAAATAGGTC GGATAATAGA GGGGAGCCCC GCGGATCGCT GCGGCAAGCT GAAAGTGGGG 
GACCGGATCT TGGCCGTTAA CGGGTGCTCC ATCACCAACA AGTCACACTC GGACATCGTC 
AATTTAATAA AGGAAGCGGG GAACACCGTG ACTCTGCGCA TCATTCCCGG CGATGAAACG 
TCCAACGCGT CTTTATTAAC CAATGCAGAG AAGATTGCCA CGATTACGAC CACGCATACG 
CCACAAACGA TACCCCAAGA AACAAAAAAT AATGCAAAAC CAAAGCCGGA GCCCCAGTTT 
GAATTCAAGA ACTCACCAGC CACACAGCCT GATCAAGAAA TTTATTCAGT GGAACTAGAC 
CGAGGATTAA AAGGATTTGG GTTTAGTCTA CGAGGAGGTC GAGAATATAA CATGGATCTC 
TATGTTTTAC GCCTTGCGGA GGATGGGCCT GCAGCCAGAT GTGGGAAAAT GAGGGTTGGC 
GATGAAATCC TGGAAATAAA CGGAGAGACC ACAAAGAACA TGAAGCACGC CCGGGCAATA 
GAACTGATTA AAAATGGTGG CCGAAGAGTA CGCCTGTGTC TGAAACGCGG AGATGGTTCC 
GTACCGGAAT ATGGTGGGTC AAACTTTGAA AACATTCCTT TGCTTCCTGC CGTCACTCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_031901241.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757101.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OXa57636
Clone ID Related Accession (Same CDS sequence)	XM_031901241.1
Accession Version	XM_031901241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAATTGCC CGCTGGATGG GAGAAAATTG AAGACCCGGT TTATGGAGTC 
TACTATGTAG ATCACATAAA CAAGAAAACG CAGTATGAAA ATCCTGTATT GGAGGCCAAA 
CGAAAGAAAC AGCTTGGACA GCAGCAAAGT GAAGAATGGA CAGGGGACCA TCCAACCATT 
GCTCCGCCCC CCATAATGCT TCCTAACAAC CTTACTCCAA AAAAGAAGGA ACCAGCAAGA 
GAAACGGCAT CACATGTGAA ACCGTTCTTC ACCAGGAACC CCTCTGAACT GAAAGGCAAG 
TTCATCCATA CGAAGCTAAG GAAAAGTAGT CGGGGCTTTG GCTTTACCGT TGTCGGAGGG 
GACGAACCAG ATGAGTTCTT GCAAATAAAA AGTTTGGTCG TCGACGGTCC GGCCGCGTTG 
GACGGCAAAA TGGAAACGGG TGATGTTATC GTTAGCGTGA ACGATATCTG CGTCCTTGGC 
TACACGCACG CTCAAGTTGT GAAGATCTTC CAGTCTATAC CCATTGGGGA AAGTGTGGAC 
CTTGAACTAT GCCGGGGTTA TCCTCTTCCG TTTGACCCAG ATGACCCCAA CACCAGCCTT 
GTGACATCAG TTGCAATATT GGACAAAGAC CCTATCATTG TCAATGGGCA AGAGAATTAT 
GACTCTCCGT CGAGCAACAG CAGTAAAACT AGAAAAGCCA ATAACGTCAA GGATCCAAGG 
CCTAGCAGCC CTGCTGACAT TTCTTCAAAT GGTTCCCATG GTTACCCTAA TGATACAGTG 
TCCTTGGCAT CCTCTATAGC TACGCAACCT GAACTAATTA CTGTGCATAT AGTAAAAGGA 
CCAATGGGAT TTGGCTTTAC CATAGCAGAC AGTCCTGGCG GTGGGGGCCA GAGAGTGAAG 
CAGATTGTAG ATAGTCCAAG ATGCCGGGGC CTCAAAGAAG GAGATCTTAT AGTAGAGGTG 
AATAAAAAGA ATGTTCAGAA CCTTACCCAT AACCAAGTTG TGGATTTACT CATCGAATGT 
CCTAAGGGAA GCGAAGTTAC CCTACTGGTT CAAAGAGGAG GACTCCCGGT CCCAAAGAAG 
AGCCCAAAGT CGCAACCACT TGAAAGGAAA GACAGCCAAA ACAGCTCGCA GCACAGTGTT 
TCCAGCCAGC ACAGTGGGCA CACTGCTTCA CCCCATCACG CCGCGCCTGG GCTCCCCGAT 
TATCCTACCA GCGAGGCGCC GGCTGTCGAC CAAGCCGACA ACTCCGGGCA GAAAAAGCCT 
GATCCTTTCA AAATCTGGGC CCAATCCAGG AGCATGTATG AGAACAGACT TCCAGACTTT 
CAAGAGCATG ATATATTTTT GTGGCGAAAG GAAACTGGAT TTGGGTTTCG AATTCTCGGT 
GGAAATGAGC CTGGAGAACC AATCTTCATA GGTCATATTG TGCCACTGGG GTCAGCGGAT 
GCCGACGGGC GGTTGCGCTC TGGTGACGAG CTTATCTGTG TGGATGGAAC AGCGGTTGTT 
GGAAAATCTC ATCAACTCGT CGTACAGCTT ATGCAACAGG CTGCGAAGCA AGGACACGTC 
AATCTAACGA TCAGACGAAA AACGATCTAT CCGGTTCCAA AGGCAGAGAA CGAAGCTCCT 
GCATCACCAG CATCCTCGCA CCATAGCAGC ACCCAGCCGG CGTCTCTAAC GGAGGAGAAG 
CGGACGCCCC AAGGCAGCCA AAACTCCTTA AACACTGTCA GTTCAGGCAG CGGCAGCACC 
AGCGGCATTG GCAGTGGCGG AGGCGGCGGG AGCGGCAACG TCAGCACCGT GGTTCAGCCG 
TATGATGTCG AGATCCGACG CGGTGAAAAT GAAGGATTTG GATTTGTCAT TGTGTCTTCT 
GTCAGCCGGC CAGAAGCCGG GACTACTTTT GCCGGCAATG CATGTGTGGC CATGCCTCAC 
AAAATAGGTC GGATAATAGA GGGGAGCCCC GCGGATCGCT GCGGCAAGCT GAAAGTGGGG 
GACCGGATCT TGGCCGTTAA CGGGTGCTCC ATCACCAACA AGTCACACTC GGACATCGTC 
AATTTAATAA AGGAAGCGGG GAACACCGTG ACTCTGCGCA TCATTCCCGG CGATGAAACG 
TCCAACGCGT CTTTATTAAC CAATGCAGAG AAGATTGCCA CGATTACGAC CACGCATACG 
CCACAAACGA TACCCCAAGA AACAAAAAAT AATGCAAAAC CAAAGCCGGA GCCCCAGTTT 
GAATTCAAGA ACTCACCAGC CACACAGCCT GATCAAGAAA TTTATTCAGT GGAACTAGAC 
CGAGGATTAA AAGGATTTGG GTTTAGTCTA CGAGGAGGTC GAGAATATAA CATGGATCTC 
TATGTTTTAC GCCTTGCGGA GGATGGGCCT GCAGCCAGAT GTGGGAAAAT GAGGGTTGGC 
GATGAAATCC TGGAAATAAA CGGAGAGACC ACAAAGAACA TGAAGCACGC CCGGGCAATA 
GAACTGATTA AAAATGGTGG CCGAAGAGTA CGCCTGTGTC TGAAACGCGG AGATGGTTCC 
GTACCGGAAT ATGCGATGAT TCCTCCTAAT ATCGCTGCAT GTATGAGAAA TGACAAGCTC 
GGGGAACCTT GCTTCTACCT AATGGGCCAA AATCAAACTA CGAACCCGGC GTTGACGGAA 
ACAGCCCCAC AACCAGTCCA CAAAGTGTAT CGAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757101.1
CDS	278..3976
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYAMIPPNIAACMRNDKLGEPCFYLMGQNQTTNPALTETAPQPVHKVYRK

Target ORF information:

RefSeq Version	XM_031901241.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901241.1

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAATTGCC CGCTGGATGG GAGAAAATTG AAGACCCGGT TTATGGAGTC 
TACTATGTAG ATCACATAAA CAAGAAAACG CAGTATGAAA ATCCTGTATT GGAGGCCAAA 
CGAAAGAAAC AGCTTGGACA GCAGCAAAGT GAAGAATGGA CAGGGGACCA TCCAACCATT 
GCTCCGCCCC CCATAATGCT TCCTAACAAC CTTACTCCAA AAAAGAAGGA ACCAGCAAGA 
GAAACGGCAT CACATGTGAA ACCGTTCTTC ACCAGGAACC CCTCTGAACT GAAAGGCAAG 
TTCATCCATA CGAAGCTAAG GAAAAGTAGT CGGGGCTTTG GCTTTACCGT TGTCGGAGGG 
GACGAACCAG ATGAGTTCTT GCAAATAAAA AGTTTGGTCG TCGACGGTCC GGCCGCGTTG 
GACGGCAAAA TGGAAACGGG TGATGTTATC GTTAGCGTGA ACGATATCTG CGTCCTTGGC 
TACACGCACG CTCAAGTTGT GAAGATCTTC CAGTCTATAC CCATTGGGGA AAGTGTGGAC 
CTTGAACTAT GCCGGGGTTA TCCTCTTCCG TTTGACCCAG ATGACCCCAA CACCAGCCTT 
GTGACATCAG TTGCAATATT GGACAAAGAC CCTATCATTG TCAATGGGCA AGAGAATTAT 
GACTCTCCGT CGAGCAACAG CAGTAAAACT AGAAAAGCCA ATAACGTCAA GGATCCAAGG 
CCTAGCAGCC CTGCTGACAT TTCTTCAAAT GGTTCCCATG GTTACCCTAA TGATACAGTG 
TCCTTGGCAT CCTCTATAGC TACGCAACCT GAACTAATTA CTGTGCATAT AGTAAAAGGA 
CCAATGGGAT TTGGCTTTAC CATAGCAGAC AGTCCTGGCG GTGGGGGCCA GAGAGTGAAG 
CAGATTGTAG ATAGTCCAAG ATGCCGGGGC CTCAAAGAAG GAGATCTTAT AGTAGAGGTG 
AATAAAAAGA ATGTTCAGAA CCTTACCCAT AACCAAGTTG TGGATTTACT CATCGAATGT 
CCTAAGGGAA GCGAAGTTAC CCTACTGGTT CAAAGAGGAG GACTCCCGGT CCCAAAGAAG 
AGCCCAAAGT CGCAACCACT TGAAAGGAAA GACAGCCAAA ACAGCTCGCA GCACAGTGTT 
TCCAGCCAGC ACAGTGGGCA CACTGCTTCA CCCCATCACG CCGCGCCTGG GCTCCCCGAT 
TATCCTACCA GCGAGGCGCC GGCTGTCGAC CAAGCCGACA ACTCCGGGCA GAAAAAGCCT 
GATCCTTTCA AAATCTGGGC CCAATCCAGG AGCATGTATG AGAACAGACT TCCAGACTTT 
CAAGAGCATG ATATATTTTT GTGGCGAAAG GAAACTGGAT TTGGGTTTCG AATTCTCGGT 
GGAAATGAGC CTGGAGAACC AATCTTCATA GGTCATATTG TGCCACTGGG GTCAGCGGAT 
GCCGACGGGC GGTTGCGCTC TGGTGACGAG CTTATCTGTG TGGATGGAAC AGCGGTTGTT 
GGAAAATCTC ATCAACTCGT CGTACAGCTT ATGCAACAGG CTGCGAAGCA AGGACACGTC 
AATCTAACGA TCAGACGAAA AACGATCTAT CCGGTTCCAA AGGCAGAGAA CGAAGCTCCT 
GCATCACCAG CATCCTCGCA CCATAGCAGC ACCCAGCCGG CGTCTCTAAC GGAGGAGAAG 
CGGACGCCCC AAGGCAGCCA AAACTCCTTA AACACTGTCA GTTCAGGCAG CGGCAGCACC 
AGCGGCATTG GCAGTGGCGG AGGCGGCGGG AGCGGCAACG TCAGCACCGT GGTTCAGCCG 
TATGATGTCG AGATCCGACG CGGTGAAAAT GAAGGATTTG GATTTGTCAT TGTGTCTTCT 
GTCAGCCGGC CAGAAGCCGG GACTACTTTT GCCGGCAATG CATGTGTGGC CATGCCTCAC 
AAAATAGGTC GGATAATAGA GGGGAGCCCC GCGGATCGCT GCGGCAAGCT GAAAGTGGGG 
GACCGGATCT TGGCCGTTAA CGGGTGCTCC ATCACCAACA AGTCACACTC GGACATCGTC 
AATTTAATAA AGGAAGCGGG GAACACCGTG ACTCTGCGCA TCATTCCCGG CGATGAAACG 
TCCAACGCGT CTTTATTAAC CAATGCAGAG AAGATTGCCA CGATTACGAC CACGCATACG 
CCACAAACGA TACCCCAAGA AACAAAAAAT AATGCAAAAC CAAAGCCGGA GCCCCAGTTT 
GAATTCAAGA ACTCACCAGC CACACAGCCT GATCAAGAAA TTTATTCAGT GGAACTAGAC 
CGAGGATTAA AAGGATTTGG GTTTAGTCTA CGAGGAGGTC GAGAATATAA CATGGATCTC 
TATGTTTTAC GCCTTGCGGA GGATGGGCCT GCAGCCAGAT GTGGGAAAAT GAGGGTTGGC 
GATGAAATCC TGGAAATAAA CGGAGAGACC ACAAAGAACA TGAAGCACGC CCGGGCAATA 
GAACTGATTA AAAATGGTGG CCGAAGAGTA CGCCTGTGTC TGAAACGCGG AGATGGTTCC 
GTACCGGAAT ATGCGATGAT TCCTCCTAAT ATCGCTGCAT GTATGAGAAA TGACAAGCTC 
GGGGAACCTT GCTTCTACCT AATGGGCCAA AATCAAACTA CGAACCCGGC GTTGACGGAA 
ACAGCCCCAC AACCAGTCCA CAAAGTGTAT CGAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

magi1 ( XM_012961765.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817219.1 Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa33044
Clone ID Related Accession (Same CDS sequence)	XM_012961765.2
Accession Version	XM_012961765.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683372.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961765.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGAATTGCCC 
GCTGGATGGG AGAAAATTGA AGACCCGGTT TATGGAGTCT ACTATGTAGA TCACATAAAC 
AAGAAAACGC AGTATGAAAA TCCTGTATTG GAGGCCAAAC GAAAGAAACA GCTTGGACAG 
CAGCAAAGTG AAGAATGGAC AGGGGACCAT CCAACCATTG CTCCGCCCCC CATAATGCTT 
CCTAACAACC TTACTCCAAA AAAGAAGGAA CCAGCAAGAG AAACGGCATC ACATGTGAAA 
CCGTTCTTCA CCAGGAACCC CTCTGAACTG AAAGGCAAGT TCATCCATAC GAAGCTAAGG 
AAAAGTAGTC GGGGCTTTGG CTTTACCGTT GTCGGAGGGG ACGAACCAGA TGAGTTCTTG 
CAAATAAAAA GTTTGGTCGT CGACGGTCCG GCCGCGTTGG ACGGCAAAAT GGAAACGGGT 
GATGTTATCG TTAGCGTGAA CGATATCTGC GTCCTTGGCT ACACGCACGC TCAAGTTGTG 
AAGATCTTCC AGTCTATACC CATTGGGGAA AGTGTGGACC TTGAACTATG CCGGGGTTAT 
CCTCTTCCGT TTGACCCAGA TGACCCCAAC ACCAGCCTTG TGACATCAGT TGCAATATTG 
GACAAAGACC CTATCATTGT CAATGGGCAA GAGAATTATG ACTCTCCGTC GAGCAACAGC 
AGTAAAACTA GAAAAGCCAA TAACGTCAAG GATCCAAGGC CTAGCAGCCC TGCTGACATT 
TCTTCAAATG GTTCCCATGG TTACCCTAAT GATACAGTGT CCTTGGCATC CTCTATAGCT 
ACGCAACCTG AACTAATTAC TGTGCATATA GTAAAAGGAC CAATGGGATT TGGCTTTACC 
ATAGCAGACA GTCCTGGCGG TGGGGGCCAG AGAGTGAAGC AGATTGTAGA TAGTCCAAGA 
TGCCGGGGCC TCAAAGAAGG AGATCTTATA GTAGAGGTGA ATAAAAAGAA TGTTCAGAAC 
CTTACCCATA ACCAAGTTGT GGATTTACTC ATCGAATGTC CTAAGGGAAG CGAAGTTACC 
CTACTGGTTC AAAGAGGAGG ACTCCCGGTC CCAAAGAAGA GCCCAAAGTC GCAACCACTT 
GAAAGGAAAG ACAGCCAAAA CAGCTCGCAG CACAGTGTTT CCAGCCAGCA CAGTGGGCAC 
ACTGCTTCAC CCCATCACGC CGCGCCTGGG CTCCCCGATT ATCCTACCAG CGAGGCGCCG 
GCTGTCGACC AAGCCGACAA CTCCGGGCAG AAAAAGCCTG ATCCTTTCAA AATCTGGGCC 
CAATCCAGGA GCATGTATGA GAACAGACTT CCAGACTTTC AAGAGCATGA TATATTTTTG 
TGGCGAAAGG AAACTGGATT TGGGTTTCGA ATTCTCGGTG GAAATGAGCC TGGAGAACCA 
ATCTTCATAG GTCATATTGT GCCACTGGGG TCAGCGGATG CCGACGGGCG GTTGCGCTCT 
GGTGACGAGC TTATCTGTGT GGATGGAACA GCGGTTGTTG GAAAATCTCA TCAACTCGTC 
GTACAGCTTA TGCAACAGGC TGCGAAGCAA GGACACGTCA ATCTAACGAT CAGACGAAAA 
ACGATCTATC CGGTTCCAAA GGCAGAGAAC GAAGCTCCTG CATCACCAGC ATCCTCGCAC 
CATAGCAGCA CCCAGCCGGC GTCTCTAACG GAGGAGAAGC GGACGCCCCA AGGCAGCCAA 
AACTCCTTAA ACACTGTCAG TTCAGGCAGC GGCAGCACCA GCGGCATTGG CAGTGGCGGA 
GGCGGCGGGA GCGGCAACGT CAGCACCGTG GTTCAGCCGT ATGATGTCGA GATCCGACGC 
GGTGAAAATG AAGGATTTGG ATTTGTCATT GTGTCTTCTG TCAGCCGGCC AGAAGCCGGG 
ACTACTTTTG CCGGCAATGC ATGTGTGGCC ATGCCTCACA AAATAGGTCG GATAATAGAG 
GGGAGCCCCG CGGATCGCTG CGGCAAGCTG AAAGTGGGGG ACCGGATCTT GGCCGTTAAC 
GGGTGCTCCA TCACCAACAA GTCACACTCG GACATCGTCA ATTTAATAAA GGAAGCGGGG 
AACACCGTGA CTCTGCGCAT CATTCCCGGC GATGAAACGT CCAACGCGTC TTTATTAACC 
AATGCAGAGA AGATTGCCAC GATTACGACC ACGCATACGC CACAAACGAT ACCCCAAGAA 
ACAAAAAATA ATGCAAAACC AAAGCCGGAG CCCCAGTTTG AATTCAAGAA CTCACCAGCC 
ACACAGCCTG ATCAAGAAAT TTATTCAGTG GAACTAGACC GAGGATTAAA AGGATTTGGG 
TTTAGTCTAC GAGGAGGTCG AGAATATAAC ATGGATCTCT ATGTTTTACG CCTTGCGGAG 
GATGGGCCTG CAGCCAGATG TGGGAAAATG AGGGTTGGCG ATGAAATCCT GGAAATAAAC 
GGAGAGACCA CAAAGAACAT GAAGCACGCC CGGGCAATAG AACTGATTAA AAATGGTGGC 
CGAAGAGTAC GCCTGTGTCT GAAACGCGGA GATGGTTCCG TACCGGAATA TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817219.1
CDS	286..3882
Translation	MAKVIQKKNHWSSRVHRCAVSCVGEPGEPGLPVLGGAEDGEFLYLGGAAEGLRCSDGELAEGELLLEVAGVPVSGLPRYDVLELVRNSKQPVPITAVRQGSRLNKDLRHYLNQRFQKASPDHELQQIIRDNLYRHAVPCTTRSPREGEVPGVDYKFLTVQEFLELEKSGTLLEVGTYEGNYYGTPKPPSQPTSGKVISRDALQNPLAGSKQSTPKRTKSYNDMQNAGVVHTDHYEFDDDAPEMSSSFTGDSSEADEHTINLSQDKVPPAARILPTFPITEASQNLPHYLPPDEESLGPLPDNWEMAFTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINKKTQYENPVLEAKRKKQLGQQQSEEWTGDHPTIAPPPIMLPNNLTPKKKEPARETASHVKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVVDGPAALDGKMETGDVIVSVNDICVLGYTHAQVVKIFQSIPIGESVDLELCRGYPLPFDPDDPNTSLVTSVAILDKDPIIVNGQENYDSPSSNSSKTRKANNVKDPRPSSPADISSNGSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKKNVQNLTHNQVVDLLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSQHSGHTASPHHAAPGLPDYPTSEAPAVDQADNSGQKKPDPFKIWAQSRSMYENRLPDFQEHDIFLWRKETGFGFRILGGNEPGEPIFIGHIVPLGSADADGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTIRRKTIYPVPKAENEAPASPASSHHSSTQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGNVSTVVQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDETSNASLLTNAEKIATITTTHTPQTIPQETKNNAKPKPEPQFEFKNSPATQPDQEIYSVELDRGLKGFGFSLRGGREYNMDLYVLRLAEDGPAARCGKMRVGDEILEINGETTKNMKHARAIELIKNGGRRVRLCLKRGDGSVPEYE

Target ORF information:

RefSeq Version	XM_012961765.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis membrane associated guanylate kinase, WW and PDZ domain containing 1 (magi1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961765.2

ORF Insert Sequence:

ATGGCCAAAG TCATTCAGAA GAAGAACCAC TGGAGCAGCC GAGTGCACCG CTGCGCTGTG 
AGCTGTGTGG GGGAGCCGGG GGAGCCGGGG CTGCCGGTGC TGGGGGGCGC GGAGGATGGG 
GAGTTCCTGT ACCTGGGGGG GGCGGCTGAG GGGCTGAGGT GCAGCGATGG CGAGCTGGCC 
GAGGGGGAGT TACTGCTGGA GGTGGCCGGG GTGCCCGTCT CTGGATTACC GCGATACGAT 
GTACTGGAAC TGGTCCGGAA CAGCAAGCAG CCTGTCCCCA TCACTGCTGT CAGACAAGGA 
AGCCGACTCA ACAAGGACCT GAGGCATTAT CTTAACCAAA GGTTTCAGAA AGCATCTCCA 
GACCACGAGC TACAGCAGAT TATCCGAGAT AATCTCTACC GCCATGCAGT GCCTTGCACC 
ACCCGATCGC CAAGAGAAGG GGAGGTCCCG GGAGTGGACT ATAAATTTCT GACTGTGCAG 
GAGTTTTTAG AACTTGAAAA AAGTGGAACA CTACTTGAAG TCGGCACATA TGAAGGTAAC 
TATTATGGGA CGCCCAAGCC CCCGAGCCAG CCCACCAGTG GGAAAGTGAT TTCCAGGGAT 
GCTCTGCAAA ACCCTCTGGC CGGCTCCAAG CAGTCGACTC CCAAGCGAAC AAAGTCTTAC 
AATGATATGC AAAATGCAGG CGTAGTGCAT ACCGACCACT ATGAGTTTGA TGATGATGCT 
CCTGAAATGA GCAGTAGCTT TACAGGAGAC TCCAGTGAAG CAGACGAGCA CACGATTAAC 
CTTTCCCAAG ATAAAGTCCC ACCGGCGGCT CGGATACTCC CTACTTTTCC CATCACGGAA 
GCTTCTCAGA ATCTGCCTCA TTACCTACCT CCTGACGAGG AAAGCCTTGG ACCTCTACCT 
GATAACTGGG AAATGGCCTT TACAGAGAAT GGAGAAGTCT ATTTTATAGA CCATAACACT 
AAAACGACAT CTTGGTTAGA CCCCCGGTGC CTGAACAAAC AGCAGAAGCC TCTGGAAGAA 
TGTGAAGATG ATGAAGAAGG GGTACACACC GAGGAACTGG ACAGTGAACT AGAATTGCCC 
GCTGGATGGG AGAAAATTGA AGACCCGGTT TATGGAGTCT ACTATGTAGA TCACATAAAC 
AAGAAAACGC AGTATGAAAA TCCTGTATTG GAGGCCAAAC GAAAGAAACA GCTTGGACAG 
CAGCAAAGTG AAGAATGGAC AGGGGACCAT CCAACCATTG CTCCGCCCCC CATAATGCTT 
CCTAACAACC TTACTCCAAA AAAGAAGGAA CCAGCAAGAG AAACGGCATC ACATGTGAAA 
CCGTTCTTCA CCAGGAACCC CTCTGAACTG AAAGGCAAGT TCATCCATAC GAAGCTAAGG 
AAAAGTAGTC GGGGCTTTGG CTTTACCGTT GTCGGAGGGG ACGAACCAGA TGAGTTCTTG 
CAAATAAAAA GTTTGGTCGT CGACGGTCCG GCCGCGTTGG ACGGCAAAAT GGAAACGGGT 
GATGTTATCG TTAGCGTGAA CGATATCTGC GTCCTTGGCT ACACGCACGC TCAAGTTGTG 
AAGATCTTCC AGTCTATACC CATTGGGGAA AGTGTGGACC TTGAACTATG CCGGGGTTAT 
CCTCTTCCGT TTGACCCAGA TGACCCCAAC ACCAGCCTTG TGACATCAGT TGCAATATTG 
GACAAAGACC CTATCATTGT CAATGGGCAA GAGAATTATG ACTCTCCGTC GAGCAACAGC 
AGTAAAACTA GAAAAGCCAA TAACGTCAAG GATCCAAGGC CTAGCAGCCC TGCTGACATT 
TCTTCAAATG GTTCCCATGG TTACCCTAAT GATACAGTGT CCTTGGCATC CTCTATAGCT 
ACGCAACCTG AACTAATTAC TGTGCATATA GTAAAAGGAC CAATGGGATT TGGCTTTACC 
ATAGCAGACA GTCCTGGCGG TGGGGGCCAG AGAGTGAAGC AGATTGTAGA TAGTCCAAGA 
TGCCGGGGCC TCAAAGAAGG AGATCTTATA GTAGAGGTGA ATAAAAAGAA TGTTCAGAAC 
CTTACCCATA ACCAAGTTGT GGATTTACTC ATCGAATGTC CTAAGGGAAG CGAAGTTACC 
CTACTGGTTC AAAGAGGAGG ACTCCCGGTC CCAAAGAAGA GCCCAAAGTC GCAACCACTT 
GAAAGGAAAG ACAGCCAAAA CAGCTCGCAG CACAGTGTTT CCAGCCAGCA CAGTGGGCAC 
ACTGCTTCAC CCCATCACGC CGCGCCTGGG CTCCCCGATT ATCCTACCAG CGAGGCGCCG 
GCTGTCGACC AAGCCGACAA CTCCGGGCAG AAAAAGCCTG ATCCTTTCAA AATCTGGGCC 
CAATCCAGGA GCATGTATGA GAACAGACTT CCAGACTTTC AAGAGCATGA TATATTTTTG 
TGGCGAAAGG AAACTGGATT TGGGTTTCGA ATTCTCGGTG GAAATGAGCC TGGAGAACCA 
ATCTTCATAG GTCATATTGT GCCACTGGGG TCAGCGGATG CCGACGGGCG GTTGCGCTCT 
GGTGACGAGC TTATCTGTGT GGATGGAACA GCGGTTGTTG GAAAATCTCA TCAACTCGTC 
GTACAGCTTA TGCAACAGGC TGCGAAGCAA GGACACGTCA ATCTAACGAT CAGACGAAAA 
ACGATCTATC CGGTTCCAAA GGCAGAGAAC GAAGCTCCTG CATCACCAGC ATCCTCGCAC 
CATAGCAGCA CCCAGCCGGC GTCTCTAACG GAGGAGAAGC GGACGCCCCA AGGCAGCCAA 
AACTCCTTAA ACACTGTCAG TTCAGGCAGC GGCAGCACCA GCGGCATTGG CAGTGGCGGA 
GGCGGCGGGA GCGGCAACGT CAGCACCGTG GTTCAGCCGT ATGATGTCGA GATCCGACGC 
GGTGAAAATG AAGGATTTGG ATTTGTCATT GTGTCTTCTG TCAGCCGGCC AGAAGCCGGG 
ACTACTTTTG CCGGCAATGC ATGTGTGGCC ATGCCTCACA AAATAGGTCG GATAATAGAG 
GGGAGCCCCG CGGATCGCTG CGGCAAGCTG AAAGTGGGGG ACCGGATCTT GGCCGTTAAC 
GGGTGCTCCA TCACCAACAA GTCACACTCG GACATCGTCA ATTTAATAAA GGAAGCGGGG 
AACACCGTGA CTCTGCGCAT CATTCCCGGC GATGAAACGT CCAACGCGTC TTTATTAACC 
AATGCAGAGA AGATTGCCAC GATTACGACC ACGCATACGC CACAAACGAT ACCCCAAGAA 
ACAAAAAATA ATGCAAAACC AAAGCCGGAG CCCCAGTTTG AATTCAAGAA CTCACCAGCC 
ACACAGCCTG ATCAAGAAAT TTATTCAGTG GAACTAGACC GAGGATTAAA AGGATTTGGG 
TTTAGTCTAC GAGGAGGTCG AGAATATAAC ATGGATCTCT ATGTTTTACG CCTTGCGGAG 
GATGGGCCTG CAGCCAGATG TGGGAAAATG AGGGTTGGCG ATGAAATCCT GGAAATAAAC 
GGAGAGACCA CAAAGAACAT GAAGCACGCC CGGGCAATAG AACTGATTAA AAATGGTGGC 
CGAAGAGTAC GCCTGTGTCT GAAACGCGGA GATGGTTCCG TACCGGAATA TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	magi1
Entrez Gene ID	100379993
Full Name	membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms	aip3,baiap1,bap1,magi-1,tnrc19,wwp3
General protein information	Preferred Names membrane associated guanylate kinase, WW and PDZ domain containing 1 Names membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

magi1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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