arhgef25 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgef25
Entrez Gene ID	100170494
Full Name	Rho guanine nucleotide exchange factor 25
Synonyms	geft,p63rhogef
General protein information	Preferred Names Rho guanine nucleotide exchange factor 25 Names rho guanine nucleotide exchange factor 25 Rho guanine nucleotide exchange factor (GEF) 25
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012956540.2	XP_012811994.1	rho guanine nucleotide exchange factor 25 isoform X1
XM_031895731.1	XP_031751591.1	rho guanine nucleotide exchange factor 25 isoform X1
NM_001130274.1	NP_001123746.1	rho guanine nucleotide exchange factor 25
XM_012956540.3	XP_012811994.1	rho guanine nucleotide exchange factor 25 isoform X1
XM_031895732.1	XP_031751592.1	rho guanine nucleotide exchange factor 25 isoform X1
XM_018091106.1	XP_017946595.1	rho guanine nucleotide exchange factor 25 isoform X2
XM_031895733.1	XP_031751593.1	rho guanine nucleotide exchange factor 25 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100331456	XP_005162334.1
Xenopus tropicalis (tropical clawed frog)	LOC100170494	NP_001123746.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following arhgef25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa20733	NM_001130274.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa32362	XM_012956540.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa32362	XM_031895731.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa32362	XM_031895733.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa32362	XM_031895732.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa42463	XM_018091106.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OXa32362	XM_012956540.2	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgef25 ( NM_001130274.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001123746.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), mRNA.

Price & Availability↑

CloneID	OXa20733
Clone ID Related Accession (Same CDS sequence)	NM_001130274.1
Accession Version	NM_001130274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 25
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167457.1. ##Evidence-Data-START## Transcript exon combination :: BC167457.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028291, SAMD00028292 [ECO:0000348] ##Evidence-Data-END##

ATGAGAGGGG GCAACCCACG CCACGGCTGT GCTTGCCAAA ATCTCTTCCG AAAGGTCCTG 
TCTCAGTGCA GCTGCTGCTA CGCCCGGGTG AGAGAATCCT ACTCAATTGC TGGCAGCGAT 
GGGAGCATAT CAGCTTCGGT GGCTTCCATC CAACCACAAC CTTCAACTAG TTCAGCACCC 
AGTTCCCCTG GATCCAGGAG GTCAGTCAGC ACCTTGAAAA AATGGCTGAC AAACCCAGTC 
AGGAAGCTGA GCAGTGGCAG CCGTCTAGAT CAACGACAGA GTAAGAAAAT TGAAAGCAGG 
CCAAAGAGAG GTGTACCAGG CCGGAGGAGG AGTGCAGATG ATGGAGGCAG AAGAAGTTTT 
GATCTAGGGG TCTTGCGGAA AATGGATGAG TTTACCCATC CTGAGGATAA GGGTTCGGAA 
TGGAAGGAAC AGGATCACCC TGAGCGGTTG CCTGACTTAT CAATATTAAA TGACTTGCTA 
CAAGGGGTAA CTACAGAACC CAACAATAAG GATCCGCACA CATCCTACCT CTCCGATCCT 
ACGGATTCTT TGGCATCAGA ACTACCTGTT ACTCCCTCCT TCCCCCCAGC AGAAACAGAA 
GAAGAAAAGA AGAGTGCCTT AGAAAAGAGT ATGTATGTTT TAATGGAGCT TATAGAAACA 
GAAAAGATGT ATGTTGAAGA CTTGCGCCAA ATAGTGGAGG GTTACATGGC CACCATGAAT 
ACACAAGGAA TTCCAGAAGA CATGAAGGGA AAAGACAAAA TTGTTTTTGG GAATATACAC 
CAGATCTTTG ATTGGCACAA AGACTATTTT TTAAGTGAGC TTCAAAAGTG CCTTGAGGAC 
CCAAACAGAT TAGCGATTCT CTTCATCAAG CATGAGCGCC GGCTGCACAT GTATGTGGTG 
TACTGTCAGA ACAAACCCAA GTCTGAGCAC ATTGTATCAG AGTATATTGA CACCTACTTT 
GAGGAGTTAA GACAACAGCT CTCACACCGC CTTCAACTCA ACGACCTGCT TATCAAGCCA 
GTGCAGAGGA TAATGAAGTA TCAGTTGCTG CTAAAAGACT TTCTTAAATA TTACAGCAAA 
GCTGGACAAG ACACATCAGA ACTGGAGAAA GCTGTGGAAG TTATGTGTTT CGTGCCCAAG 
CGCTGTAATG ACATGATGAA TCTAGGCAGG CTTCAGGGAT TTGAGGGAAA AATTACCGCT 
CAGGGGAAGC TTCTGCAACA AGACACATTT CTGGTGAACG AGCAGGAGAG CAGCTTTCTG 
ACACGCTGCA AAGAACGCAG GGTCTTCCTC TTTGAACAAA TAATCATCTT TAGTGAACCG 
ATTGATAAAA AGAAAGATTT CTCCTTGCCT GGATACATCT TTAAAAATAG CATTAAGATT 
AGTTGCCTTG GCATTGAAGA GTCTATAGAT AATGATCCTT GTAAGTTTGT CCTGACATCA 
CGAGGTGCTG ATAGGAGCTT GGTCCGGTAC ATACTGCAGG CCTCCACGCA AGAGATCCGC 
TTGGCTTGGA TAAAAGATAT TGGACAGATC TTAGAGAGTC AGAGGAACTT TCTCAATGCC 
CTTCAGTCTC CTATTGAATA CCAACGCCGA GAAAGCAAAT CCAATAGTCT GGGCAGAAAC 
AGTAACCTCC GCATTATGGA AACTTCCCGT GGAGGGTTAA GGCCCCATTC TTCCTCTTCT 
ATGGACAGGA ACAAAGTTCC CAACCTCAAG TCATACAATT CTTCCCTGCC TTCCCTTTAT 
TTGCCAAGCA ACCCTCGACA AGACAGTAGA CAGTTTGGAA ACAAGGTACA CTCAGCTCGA 
AAAAGTTCTC ACTCAGATGC CCTCCCTTCA CCGGCAATCC TTTCCCCAAC ACACATCAGC 
CTTGACTTCC ATCATCCAGA GTCCTCTTCA CCGACAGTGT CCAATGGGAC CCCACAAAGT 
GACTCCACCA GCCGGAAACC AAGCCTATCA GAACGTGAAC TACCTGGCTA TGCATCGTCT 
CCTGAAGATT ATGGTGTTCC TACTCCCAGG TTGGCCAAGC TGGATGAAGA TGAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123746.1
CDS	351..2390
Translation	MRGGNPRHGCACQNLFRKVLSQCSCCYARVRESYSIAGSDGSISASVASIQPQPSTSSAPSSPGSRRSVSTLKKWLTNPVRKLSSGSRLDQRQSKKIESRPKRGVPGRRRSADDGGRRSFDLGVLRKMDEFTHPEDKGSEWKEQDHPERLPDLSILNDLLQGVTTEPNNKDPHTSYLSDPTDSLASELPVTPSFPPAETEEEKKSALEKSMYVLMELIETEKMYVEDLRQIVEGYMATMNTQGIPEDMKGKDKIVFGNIHQIFDWHKDYFLSELQKCLEDPNRLAILFIKHERRLHMYVVYCQNKPKSEHIVSEYIDTYFEELRQQLSHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYSKAGQDTSELEKAVEVMCFVPKRCNDMMNLGRLQGFEGKITAQGKLLQQDTFLVNEQESSFLTRCKERRVFLFEQIIIFSEPIDKKKDFSLPGYIFKNSIKISCLGIEESIDNDPCKFVLTSRGADRSLVRYILQASTQEIRLAWIKDIGQILESQRNFLNALQSPIEYQRRESKSNSLGRNSNLRIMETSRGGLRPHSSSSMDRNKVPNLKSYNSSLPSLYLPSNPRQDSRQFGNKVHSARKSSHSDALPSPAILSPTHISLDFHHPESSSPTVSNGTPQSDSTSRKPSLSERELPGYASSPEDYGVPTPRLAKLDEDEL

Target ORF information:

RefSeq Version	NM_001130274.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130274.1

ORF Insert Sequence:

ATGAGAGGGG GCAACCCACG CCACGGCTGT GCTTGCCAAA ATCTCTTCCG AAAGGTCCTG 
TCTCAGTGCA GCTGCTGCTA CGCCCGGGTG AGAGAATCCT ACTCAATTGC TGGCAGCGAT 
GGGAGCATAT CAGCTTCGGT GGCTTCCATC CAACCACAAC CTTCAACTAG TTCAGCACCC 
AGTTCCCCTG GATCCAGGAG GTCAGTCAGC ACCTTGAAAA AATGGCTGAC AAACCCAGTC 
AGGAAGCTGA GCAGTGGCAG CCGTCTAGAT CAACGACAGA GTAAGAAAAT TGAAAGCAGG 
CCAAAGAGAG GTGTACCAGG CCGGAGGAGG AGTGCAGATG ATGGAGGCAG AAGAAGTTTT 
GATCTAGGGG TCTTGCGGAA AATGGATGAG TTTACCCATC CTGAGGATAA GGGTTCGGAA 
TGGAAGGAAC AGGATCACCC TGAGCGGTTG CCTGACTTAT CAATATTAAA TGACTTGCTA 
CAAGGGGTAA CTACAGAACC CAACAATAAG GATCCGCACA CATCCTACCT CTCCGATCCT 
ACGGATTCTT TGGCATCAGA ACTACCTGTT ACTCCCTCCT TCCCCCCAGC AGAAACAGAA 
GAAGAAAAGA AGAGTGCCTT AGAAAAGAGT ATGTATGTTT TAATGGAGCT TATAGAAACA 
GAAAAGATGT ATGTTGAAGA CTTGCGCCAA ATAGTGGAGG GTTACATGGC CACCATGAAT 
ACACAAGGAA TTCCAGAAGA CATGAAGGGA AAAGACAAAA TTGTTTTTGG GAATATACAC 
CAGATCTTTG ATTGGCACAA AGACTATTTT TTAAGTGAGC TTCAAAAGTG CCTTGAGGAC 
CCAAACAGAT TAGCGATTCT CTTCATCAAG CATGAGCGCC GGCTGCACAT GTATGTGGTG 
TACTGTCAGA ACAAACCCAA GTCTGAGCAC ATTGTATCAG AGTATATTGA CACCTACTTT 
GAGGAGTTAA GACAACAGCT CTCACACCGC CTTCAACTCA ACGACCTGCT TATCAAGCCA 
GTGCAGAGGA TAATGAAGTA TCAGTTGCTG CTAAAAGACT TTCTTAAATA TTACAGCAAA 
GCTGGACAAG ACACATCAGA ACTGGAGAAA GCTGTGGAAG TTATGTGTTT CGTGCCCAAG 
CGCTGTAATG ACATGATGAA TCTAGGCAGG CTTCAGGGAT TTGAGGGAAA AATTACCGCT 
CAGGGGAAGC TTCTGCAACA AGACACATTT CTGGTGAACG AGCAGGAGAG CAGCTTTCTG 
ACACGCTGCA AAGAACGCAG GGTCTTCCTC TTTGAACAAA TAATCATCTT TAGTGAACCG 
ATTGATAAAA AGAAAGATTT CTCCTTGCCT GGATACATCT TTAAAAATAG CATTAAGATT 
AGTTGCCTTG GCATTGAAGA GTCTATAGAT AATGATCCTT GTAAGTTTGT CCTGACATCA 
CGAGGTGCTG ATAGGAGCTT GGTCCGGTAC ATACTGCAGG CCTCCACGCA AGAGATCCGC 
TTGGCTTGGA TAAAAGATAT TGGACAGATC TTAGAGAGTC AGAGGAACTT TCTCAATGCC 
CTTCAGTCTC CTATTGAATA CCAACGCCGA GAAAGCAAAT CCAATAGTCT GGGCAGAAAC 
AGTAACCTCC GCATTATGGA AACTTCCCGT GGAGGGTTAA GGCCCCATTC TTCCTCTTCT 
ATGGACAGGA ACAAAGTTCC CAACCTCAAG TCATACAATT CTTCCCTGCC TTCCCTTTAT 
TTGCCAAGCA ACCCTCGACA AGACAGTAGA CAGTTTGGAA ACAAGGTACA CTCAGCTCGA 
AAAAGTTCTC ACTCAGATGC CCTCCCTTCA CCGGCAATCC TTTCCCCAAC ACACATCAGC 
CTTGACTTCC ATCATCCAGA GTCCTCTTCA CCGACAGTGT CCAATGGGAC CCCACAAAGT 
GACTCCACCA GCCGGAAACC AAGCCTATCA GAACGTGAAC TACCTGGCTA TGCATCGTCT 
CCTGAAGATT ATGGTGTTCC TACTCCCAGG TTGGCCAAGC TGGATGAAGA TGAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef25 ( XM_012956540.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811994.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa32362
Clone ID Related Accession (Same CDS sequence)	XM_012956540.2 , XM_031895731.1 , XM_012956540.3 , XM_031895732.1 , XM_031895733.1
Accession Version	XM_012956540.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC CTCAGCGCTC AGGGGGGCGC ACTGATCAGA TGCTCGGCGC TATGGCAGAC 
ATCCTGGGAT CATTTGGACT TCCGAGCCAC CAGTGGCTTC CAGACTCAGC CCCTGAGCAA 
AGTAGGAACG AAGAGAAGAC CGACGGGGAG AATTCCCCAG ATGAAGAGGA AGAACAACCC 
AATGTGGACC CACAACATAC AGCATCTGGT CTCCAAGTTC CTACTGATAG CACATGCCCT 
GGGATGGGCA GAACCAGACG CCTCTCGGAC TTCCGTGCGT GGTTTGGAGA AAACTTCTTT 
CACACTTCAG AAGAAGAATC CTACTCAATT GCTGGCAGCG ATGGGAGCAT ATCAGCTTCG 
GTGGCTTCCA TCCAACCACA ACCTTCAACT AGTTCAGCAC CCAGTTCCCC TGGATCCAGG 
AGGTCAGTCA GCACCTTGAA AAAATGGCTG ACAAACCCAG TCAGGAAGCT GAGCAGTGGC 
AGCCGTCTAG ATCAACGACA GAGTAAGAAA ATTGAAAGCA GGCCAAAGAG AGGTGTACCA 
GGCCGGAGGA GGAGTGCAGA TGATGGAGGC AGAAGAAGTT TTGATCTAGG GGTCTTGCGG 
AAAATGGATG AGTTTACCCA TCCTGAGGAT AAGGGTTCGG AATGGAAGGA ACAGGATCAC 
CCTGAGCGGT TGCCTGACTT ATCAATATTA AATGACTTGC TACAAGGGGT AACTACAGAA 
CCCAACAATA AGGATCCGCA CACATCCTAC CTCTCCGATC CTACGGATTC TTTGGCATCA 
GAACTACCTG TTACTCCCTC CTTCCCCCCA GCAGAAACAG AAGAAGAAAA GAAGAGTGCC 
TTAGAAAAGA GTATGTATGT TTTAATGGAG CTTATAGAAA CAGAAAAGAT GTATGTTGAA 
GACTTGCGCC AAATAGTGGA GGGTTACATG GCCACCATGA ATACACAAGG AATTCCAGAA 
GACATGAAGG GAAAAGACAA AATTGTTTTT GGGAATATAC ACCAGATCTT TGATTGGCAC 
AAAGACTATT TTTTAAGTGA GCTTCAAAAG TGCCTTGAGG ACCCAAACAG ATTAGCGATT 
CTCTTCATCA AGCATGAGCG CCGGCTGCAC ATGTATGTGG TGTACTGTCA GAACAAACCC 
AAGTCTGAGC ACATTGTATC AGAGTATATT GACACCTACT TTGAGGAGTT AAGACAACAG 
CTCTCACACC GCCTTCAACT CAACGACCTG CTTATCAAGC CAGTGCAGAG GATAATGAAG 
TATCAGTTGC TGCTAAAAGA CTTTCTTAAA TATTACAGCA AAGCTGGACA AGACACATCA 
GAACTGGAGA AAGCTGTGGA AGTTATGTGT TTCGTGCCCA AGCGCTGTAA TGACATGATG 
AATCTAGGCA GGCTTCAGGG ATTTGAGGGA AAAATTACCG CTCAGGGGAA GCTTCTGCAA 
CAAGACACAT TTCTGGTGAA CGAGCAGGAG AGCAGCTTTC TGACACGCTG CAAAGAACGC 
AGGGTCTTCC TCTTTGAACA AATAATCATC TTTAGTGAAC CGATTGATAA AAAGAAAGAT 
TTCTCCTTGC CTGGATACAT CTTTAAAAAT AGCATTAAGA TTAGTTGCCT TGGCATTGAA 
GAGTCTATAG ATAATGATCC TTGTAAGTTT GTCCTGACAT CACGAGGTGC TGATAGGAGC 
TTGGTCCGGT ACATACTGCA GGCCTCCACG CAAGAGATCC GCTTGGCTTG GATAAAAGAT 
ATTGGACAGA TCTTAGAGAG TCAGAGGAAC TTTCTCAATG CCCTTCAGTC TCCTATTGAA 
TACCAACGCC GAGAAAGCAA ATCCAATAGT CTGGGCAGAA ACAGTAACCT CCGCATTATG 
GAAACTTCCC GTGGAGGGTT AAGGCCCCAT TCTTCCTCTT CTATGGACAG GAACAAAGTT 
CCCAACCTCA AGTCATACAA TTCTTCCCTG CCTTCCCTTT ATTTGCCAAG CAACCCTCGA 
CAAGACAGTA GACAGTTTGG AAACAAGGTA CACTCAGCTC GAAAAAGTTC TCACTCAGAT 
GCCCTCCCTT CACCGGCAAT CCTTTCCCCA ACACACATCA GCCTTGACTT CCATCATCCA 
GAGTCCTCTT CACCGACAGT GTCCAATGGG ACCCCACAAA GTGACTCCAC CAGCCGGAAA 
CCAAGCCTAT CAGAACGTGA ACTACCTGGC TATGCATCGT CTCCTGAAGA TTATGGTGTT 
CCTACTCCCA GGTTGGCCAA GCTGGATGAA GATGAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811994.1
CDS	594..2855
Translation	MKAPQRSGGRTDQMLGAMADILGSFGLPSHQWLPDSAPEQSRNEEKTDGENSPDEEEEQPNVDPQHTASGLQVPTDSTCPGMGRTRRLSDFRAWFGENFFHTSEEESYSIAGSDGSISASVASIQPQPSTSSAPSSPGSRRSVSTLKKWLTNPVRKLSSGSRLDQRQSKKIESRPKRGVPGRRRSADDGGRRSFDLGVLRKMDEFTHPEDKGSEWKEQDHPERLPDLSILNDLLQGVTTEPNNKDPHTSYLSDPTDSLASELPVTPSFPPAETEEEKKSALEKSMYVLMELIETEKMYVEDLRQIVEGYMATMNTQGIPEDMKGKDKIVFGNIHQIFDWHKDYFLSELQKCLEDPNRLAILFIKHERRLHMYVVYCQNKPKSEHIVSEYIDTYFEELRQQLSHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYSKAGQDTSELEKAVEVMCFVPKRCNDMMNLGRLQGFEGKITAQGKLLQQDTFLVNEQESSFLTRCKERRVFLFEQIIIFSEPIDKKKDFSLPGYIFKNSIKISCLGIEESIDNDPCKFVLTSRGADRSLVRYILQASTQEIRLAWIKDIGQILESQRNFLNALQSPIEYQRRESKSNSLGRNSNLRIMETSRGGLRPHSSSSMDRNKVPNLKSYNSSLPSLYLPSNPRQDSRQFGNKVHSARKSSHSDALPSPAILSPTHISLDFHHPESSSPTVSNGTPQSDSTSRKPSLSERELPGYASSPEDYGVPTPRLAKLDEDEL

Target ORF information:

RefSeq Version	XM_012956540.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956540.2

ORF Insert Sequence:

ATGAAGGCAC CTCAGCGCTC AGGGGGGCGC ACTGATCAGA TGCTCGGCGC TATGGCAGAC 
ATCCTGGGAT CATTTGGACT TCCGAGCCAC CAGTGGCTTC CAGACTCAGC CCCTGAGCAA 
AGTAGGAACG AAGAGAAGAC CGACGGGGAG AATTCCCCAG ATGAAGAGGA AGAACAACCC 
AATGTGGACC CACAACATAC AGCATCTGGT CTCCAAGTTC CTACTGATAG CACATGCCCT 
GGGATGGGCA GAACCAGACG CCTCTCGGAC TTCCGTGCGT GGTTTGGAGA AAACTTCTTT 
CACACTTCAG AAGAAGAATC CTACTCAATT GCTGGCAGCG ATGGGAGCAT ATCAGCTTCG 
GTGGCTTCCA TCCAACCACA ACCTTCAACT AGTTCAGCAC CCAGTTCCCC TGGATCCAGG 
AGGTCAGTCA GCACCTTGAA AAAATGGCTG ACAAACCCAG TCAGGAAGCT GAGCAGTGGC 
AGCCGTCTAG ATCAACGACA GAGTAAGAAA ATTGAAAGCA GGCCAAAGAG AGGTGTACCA 
GGCCGGAGGA GGAGTGCAGA TGATGGAGGC AGAAGAAGTT TTGATCTAGG GGTCTTGCGG 
AAAATGGATG AGTTTACCCA TCCTGAGGAT AAGGGTTCGG AATGGAAGGA ACAGGATCAC 
CCTGAGCGGT TGCCTGACTT ATCAATATTA AATGACTTGC TACAAGGGGT AACTACAGAA 
CCCAACAATA AGGATCCGCA CACATCCTAC CTCTCCGATC CTACGGATTC TTTGGCATCA 
GAACTACCTG TTACTCCCTC CTTCCCCCCA GCAGAAACAG AAGAAGAAAA GAAGAGTGCC 
TTAGAAAAGA GTATGTATGT TTTAATGGAG CTTATAGAAA CAGAAAAGAT GTATGTTGAA 
GACTTGCGCC AAATAGTGGA GGGTTACATG GCCACCATGA ATACACAAGG AATTCCAGAA 
GACATGAAGG GAAAAGACAA AATTGTTTTT GGGAATATAC ACCAGATCTT TGATTGGCAC 
AAAGACTATT TTTTAAGTGA GCTTCAAAAG TGCCTTGAGG ACCCAAACAG ATTAGCGATT 
CTCTTCATCA AGCATGAGCG CCGGCTGCAC ATGTATGTGG TGTACTGTCA GAACAAACCC 
AAGTCTGAGC ACATTGTATC AGAGTATATT GACACCTACT TTGAGGAGTT AAGACAACAG 
CTCTCACACC GCCTTCAACT CAACGACCTG CTTATCAAGC CAGTGCAGAG GATAATGAAG 
TATCAGTTGC TGCTAAAAGA CTTTCTTAAA TATTACAGCA AAGCTGGACA AGACACATCA 
GAACTGGAGA AAGCTGTGGA AGTTATGTGT TTCGTGCCCA AGCGCTGTAA TGACATGATG 
AATCTAGGCA GGCTTCAGGG ATTTGAGGGA AAAATTACCG CTCAGGGGAA GCTTCTGCAA 
CAAGACACAT TTCTGGTGAA CGAGCAGGAG AGCAGCTTTC TGACACGCTG CAAAGAACGC 
AGGGTCTTCC TCTTTGAACA AATAATCATC TTTAGTGAAC CGATTGATAA AAAGAAAGAT 
TTCTCCTTGC CTGGATACAT CTTTAAAAAT AGCATTAAGA TTAGTTGCCT TGGCATTGAA 
GAGTCTATAG ATAATGATCC TTGTAAGTTT GTCCTGACAT CACGAGGTGC TGATAGGAGC 
TTGGTCCGGT ACATACTGCA GGCCTCCACG CAAGAGATCC GCTTGGCTTG GATAAAAGAT 
ATTGGACAGA TCTTAGAGAG TCAGAGGAAC TTTCTCAATG CCCTTCAGTC TCCTATTGAA 
TACCAACGCC GAGAAAGCAA ATCCAATAGT CTGGGCAGAA ACAGTAACCT CCGCATTATG 
GAAACTTCCC GTGGAGGGTT AAGGCCCCAT TCTTCCTCTT CTATGGACAG GAACAAAGTT 
CCCAACCTCA AGTCATACAA TTCTTCCCTG CCTTCCCTTT ATTTGCCAAG CAACCCTCGA 
CAAGACAGTA GACAGTTTGG AAACAAGGTA CACTCAGCTC GAAAAAGTTC TCACTCAGAT 
GCCCTCCCTT CACCGGCAAT CCTTTCCCCA ACACACATCA GCCTTGACTT CCATCATCCA 
GAGTCCTCTT CACCGACAGT GTCCAATGGG ACCCCACAAA GTGACTCCAC CAGCCGGAAA 
CCAAGCCTAT CAGAACGTGA ACTACCTGGC TATGCATCGT CTCCTGAAGA TTATGGTGTT 
CCTACTCCCA GGTTGGCCAA GCTGGATGAA GATGAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef25 ( XM_031895731.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751591.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa32362
Clone ID Related Accession (Same CDS sequence)	XM_012956540.2 , XM_031895731.1 , XM_012956540.3 , XM_031895732.1 , XM_031895733.1
Accession Version	XM_031895731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC CTCAGCGCTC AGGGGGGCGC ACTGATCAGA TGCTCGGCGC TATGGCAGAC 
ATCCTGGGAT CATTTGGACT TCCGAGCCAC CAGTGGCTTC CAGACTCAGC CCCTGAGCAA 
AGTAGGAACG AAGAGAAGAC CGACGGGGAG AATTCCCCAG ATGAAGAGGA AGAACAACCC 
AATGTGGACC CACAACATAC AGCATCTGGT CTCCAAGTTC CTACTGATAG CACATGCCCT 
GGGATGGGCA GAACCAGACG CCTCTCGGAC TTCCGTGCGT GGTTTGGAGA AAACTTCTTT 
CACACTTCAG AAGAAGAATC CTACTCAATT GCTGGCAGCG ATGGGAGCAT ATCAGCTTCG 
GTGGCTTCCA TCCAACCACA ACCTTCAACT AGTTCAGCAC CCAGTTCCCC TGGATCCAGG 
AGGTCAGTCA GCACCTTGAA AAAATGGCTG ACAAACCCAG TCAGGAAGCT GAGCAGTGGC 
AGCCGTCTAG ATCAACGACA GAGTAAGAAA ATTGAAAGCA GGCCAAAGAG AGGTGTACCA 
GGCCGGAGGA GGAGTGCAGA TGATGGAGGC AGAAGAAGTT TTGATCTAGG GGTCTTGCGG 
AAAATGGATG AGTTTACCCA TCCTGAGGAT AAGGGTTCGG AATGGAAGGA ACAGGATCAC 
CCTGAGCGGT TGCCTGACTT ATCAATATTA AATGACTTGC TACAAGGGGT AACTACAGAA 
CCCAACAATA AGGATCCGCA CACATCCTAC CTCTCCGATC CTACGGATTC TTTGGCATCA 
GAACTACCTG TTACTCCCTC CTTCCCCCCA GCAGAAACAG AAGAAGAAAA GAAGAGTGCC 
TTAGAAAAGA GTATGTATGT TTTAATGGAG CTTATAGAAA CAGAAAAGAT GTATGTTGAA 
GACTTGCGCC AAATAGTGGA GGGTTACATG GCCACCATGA ATACACAAGG AATTCCAGAA 
GACATGAAGG GAAAAGACAA AATTGTTTTT GGGAATATAC ACCAGATCTT TGATTGGCAC 
AAAGACTATT TTTTAAGTGA GCTTCAAAAG TGCCTTGAGG ACCCAAACAG ATTAGCGATT 
CTCTTCATCA AGCATGAGCG CCGGCTGCAC ATGTATGTGG TGTACTGTCA GAACAAACCC 
AAGTCTGAGC ACATTGTATC AGAGTATATT GACACCTACT TTGAGGAGTT AAGACAACAG 
CTCTCACACC GCCTTCAACT CAACGACCTG CTTATCAAGC CAGTGCAGAG GATAATGAAG 
TATCAGTTGC TGCTAAAAGA CTTTCTTAAA TATTACAGCA AAGCTGGACA AGACACATCA 
GAACTGGAGA AAGCTGTGGA AGTTATGTGT TTCGTGCCCA AGCGCTGTAA TGACATGATG 
AATCTAGGCA GGCTTCAGGG ATTTGAGGGA AAAATTACCG CTCAGGGGAA GCTTCTGCAA 
CAAGACACAT TTCTGGTGAA CGAGCAGGAG AGCAGCTTTC TGACACGCTG CAAAGAACGC 
AGGGTCTTCC TCTTTGAACA AATAATCATC TTTAGTGAAC CGATTGATAA AAAGAAAGAT 
TTCTCCTTGC CTGGATACAT CTTTAAAAAT AGCATTAAGA TTAGTTGCCT TGGCATTGAA 
GAGTCTATAG ATAATGATCC TTGTAAGTTT GTCCTGACAT CACGAGGTGC TGATAGGAGC 
TTGGTCCGGT ACATACTGCA GGCCTCCACG CAAGAGATCC GCTTGGCTTG GATAAAAGAT 
ATTGGACAGA TCTTAGAGAG TCAGAGGAAC TTTCTCAATG CCCTTCAGTC TCCTATTGAA 
TACCAACGCC GAGAAAGCAA ATCCAATAGT CTGGGCAGAA ACAGTAACCT CCGCATTATG 
GAAACTTCCC GTGGAGGGTT AAGGCCCCAT TCTTCCTCTT CTATGGACAG GAACAAAGTT 
CCCAACCTCA AGTCATACAA TTCTTCCCTG CCTTCCCTTT ATTTGCCAAG CAACCCTCGA 
CAAGACAGTA GACAGTTTGG AAACAAGGTA CACTCAGCTC GAAAAAGTTC TCACTCAGAT 
GCCCTCCCTT CACCGGCAAT CCTTTCCCCA ACACACATCA GCCTTGACTT CCATCATCCA 
GAGTCCTCTT CACCGACAGT GTCCAATGGG ACCCCACAAA GTGACTCCAC CAGCCGGAAA 
CCAAGCCTAT CAGAACGTGA ACTACCTGGC TATGCATCGT CTCCTGAAGA TTATGGTGTT 
CCTACTCCCA GGTTGGCCAA GCTGGATGAA GATGAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751591.1
CDS	370..2631
Translation	MKAPQRSGGRTDQMLGAMADILGSFGLPSHQWLPDSAPEQSRNEEKTDGENSPDEEEEQPNVDPQHTASGLQVPTDSTCPGMGRTRRLSDFRAWFGENFFHTSEEESYSIAGSDGSISASVASIQPQPSTSSAPSSPGSRRSVSTLKKWLTNPVRKLSSGSRLDQRQSKKIESRPKRGVPGRRRSADDGGRRSFDLGVLRKMDEFTHPEDKGSEWKEQDHPERLPDLSILNDLLQGVTTEPNNKDPHTSYLSDPTDSLASELPVTPSFPPAETEEEKKSALEKSMYVLMELIETEKMYVEDLRQIVEGYMATMNTQGIPEDMKGKDKIVFGNIHQIFDWHKDYFLSELQKCLEDPNRLAILFIKHERRLHMYVVYCQNKPKSEHIVSEYIDTYFEELRQQLSHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYSKAGQDTSELEKAVEVMCFVPKRCNDMMNLGRLQGFEGKITAQGKLLQQDTFLVNEQESSFLTRCKERRVFLFEQIIIFSEPIDKKKDFSLPGYIFKNSIKISCLGIEESIDNDPCKFVLTSRGADRSLVRYILQASTQEIRLAWIKDIGQILESQRNFLNALQSPIEYQRRESKSNSLGRNSNLRIMETSRGGLRPHSSSSMDRNKVPNLKSYNSSLPSLYLPSNPRQDSRQFGNKVHSARKSSHSDALPSPAILSPTHISLDFHHPESSSPTVSNGTPQSDSTSRKPSLSERELPGYASSPEDYGVPTPRLAKLDEDEL

Target ORF information:

RefSeq Version	XM_031895731.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895731.1

ORF Insert Sequence:

ATGAAGGCAC CTCAGCGCTC AGGGGGGCGC ACTGATCAGA TGCTCGGCGC TATGGCAGAC 
ATCCTGGGAT CATTTGGACT TCCGAGCCAC CAGTGGCTTC CAGACTCAGC CCCTGAGCAA 
AGTAGGAACG AAGAGAAGAC CGACGGGGAG AATTCCCCAG ATGAAGAGGA AGAACAACCC 
AATGTGGACC CACAACATAC AGCATCTGGT CTCCAAGTTC CTACTGATAG CACATGCCCT 
GGGATGGGCA GAACCAGACG CCTCTCGGAC TTCCGTGCGT GGTTTGGAGA AAACTTCTTT 
CACACTTCAG AAGAAGAATC CTACTCAATT GCTGGCAGCG ATGGGAGCAT ATCAGCTTCG 
GTGGCTTCCA TCCAACCACA ACCTTCAACT AGTTCAGCAC CCAGTTCCCC TGGATCCAGG 
AGGTCAGTCA GCACCTTGAA AAAATGGCTG ACAAACCCAG TCAGGAAGCT GAGCAGTGGC 
AGCCGTCTAG ATCAACGACA GAGTAAGAAA ATTGAAAGCA GGCCAAAGAG AGGTGTACCA 
GGCCGGAGGA GGAGTGCAGA TGATGGAGGC AGAAGAAGTT TTGATCTAGG GGTCTTGCGG 
AAAATGGATG AGTTTACCCA TCCTGAGGAT AAGGGTTCGG AATGGAAGGA ACAGGATCAC 
CCTGAGCGGT TGCCTGACTT ATCAATATTA AATGACTTGC TACAAGGGGT AACTACAGAA 
CCCAACAATA AGGATCCGCA CACATCCTAC CTCTCCGATC CTACGGATTC TTTGGCATCA 
GAACTACCTG TTACTCCCTC CTTCCCCCCA GCAGAAACAG AAGAAGAAAA GAAGAGTGCC 
TTAGAAAAGA GTATGTATGT TTTAATGGAG CTTATAGAAA CAGAAAAGAT GTATGTTGAA 
GACTTGCGCC AAATAGTGGA GGGTTACATG GCCACCATGA ATACACAAGG AATTCCAGAA 
GACATGAAGG GAAAAGACAA AATTGTTTTT GGGAATATAC ACCAGATCTT TGATTGGCAC 
AAAGACTATT TTTTAAGTGA GCTTCAAAAG TGCCTTGAGG ACCCAAACAG ATTAGCGATT 
CTCTTCATCA AGCATGAGCG CCGGCTGCAC ATGTATGTGG TGTACTGTCA GAACAAACCC 
AAGTCTGAGC ACATTGTATC AGAGTATATT GACACCTACT TTGAGGAGTT AAGACAACAG 
CTCTCACACC GCCTTCAACT CAACGACCTG CTTATCAAGC CAGTGCAGAG GATAATGAAG 
TATCAGTTGC TGCTAAAAGA CTTTCTTAAA TATTACAGCA AAGCTGGACA AGACACATCA 
GAACTGGAGA AAGCTGTGGA AGTTATGTGT TTCGTGCCCA AGCGCTGTAA TGACATGATG 
AATCTAGGCA GGCTTCAGGG ATTTGAGGGA AAAATTACCG CTCAGGGGAA GCTTCTGCAA 
CAAGACACAT TTCTGGTGAA CGAGCAGGAG AGCAGCTTTC TGACACGCTG CAAAGAACGC 
AGGGTCTTCC TCTTTGAACA AATAATCATC TTTAGTGAAC CGATTGATAA AAAGAAAGAT 
TTCTCCTTGC CTGGATACAT CTTTAAAAAT AGCATTAAGA TTAGTTGCCT TGGCATTGAA 
GAGTCTATAG ATAATGATCC TTGTAAGTTT GTCCTGACAT CACGAGGTGC TGATAGGAGC 
TTGGTCCGGT ACATACTGCA GGCCTCCACG CAAGAGATCC GCTTGGCTTG GATAAAAGAT 
ATTGGACAGA TCTTAGAGAG TCAGAGGAAC TTTCTCAATG CCCTTCAGTC TCCTATTGAA 
TACCAACGCC GAGAAAGCAA ATCCAATAGT CTGGGCAGAA ACAGTAACCT CCGCATTATG 
GAAACTTCCC GTGGAGGGTT AAGGCCCCAT TCTTCCTCTT CTATGGACAG GAACAAAGTT 
CCCAACCTCA AGTCATACAA TTCTTCCCTG CCTTCCCTTT ATTTGCCAAG CAACCCTCGA 
CAAGACAGTA GACAGTTTGG AAACAAGGTA CACTCAGCTC GAAAAAGTTC TCACTCAGAT 
GCCCTCCCTT CACCGGCAAT CCTTTCCCCA ACACACATCA GCCTTGACTT CCATCATCCA 
GAGTCCTCTT CACCGACAGT GTCCAATGGG ACCCCACAAA GTGACTCCAC CAGCCGGAAA 
CCAAGCCTAT CAGAACGTGA ACTACCTGGC TATGCATCGT CTCCTGAAGA TTATGGTGTT 
CCTACTCCCA GGTTGGCCAA GCTGGATGAA GATGAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef25 ( XM_012956540.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811994.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X1, mRNA.

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CloneID	OXa32362
Clone ID Related Accession (Same CDS sequence)	XM_012956540.2 , XM_031895731.1 , XM_012956540.3 , XM_031895732.1 , XM_031895733.1
Accession Version	XM_012956540.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956540.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC CTCAGCGCTC AGGGGGGCGC ACTGATCAGA TGCTCGGCGC TATGGCAGAC 
ATCCTGGGAT CATTTGGACT TCCGAGCCAC CAGTGGCTTC CAGACTCAGC CCCTGAGCAA 
AGTAGGAACG AAGAGAAGAC CGACGGGGAG AATTCCCCAG ATGAAGAGGA AGAACAACCC 
AATGTGGACC CACAACATAC AGCATCTGGT CTCCAAGTTC CTACTGATAG CACATGCCCT 
GGGATGGGCA GAACCAGACG CCTCTCGGAC TTCCGTGCGT GGTTTGGAGA AAACTTCTTT 
CACACTTCAG AAGAAGAATC CTACTCAATT GCTGGCAGCG ATGGGAGCAT ATCAGCTTCG 
GTGGCTTCCA TCCAACCACA ACCTTCAACT AGTTCAGCAC CCAGTTCCCC TGGATCCAGG 
AGGTCAGTCA GCACCTTGAA AAAATGGCTG ACAAACCCAG TCAGGAAGCT GAGCAGTGGC 
AGCCGTCTAG ATCAACGACA GAGTAAGAAA ATTGAAAGCA GGCCAAAGAG AGGTGTACCA 
GGCCGGAGGA GGAGTGCAGA TGATGGAGGC AGAAGAAGTT TTGATCTAGG GGTCTTGCGG 
AAAATGGATG AGTTTACCCA TCCTGAGGAT AAGGGTTCGG AATGGAAGGA ACAGGATCAC 
CCTGAGCGGT TGCCTGACTT ATCAATATTA AATGACTTGC TACAAGGGGT AACTACAGAA 
CCCAACAATA AGGATCCGCA CACATCCTAC CTCTCCGATC CTACGGATTC TTTGGCATCA 
GAACTACCTG TTACTCCCTC CTTCCCCCCA GCAGAAACAG AAGAAGAAAA GAAGAGTGCC 
TTAGAAAAGA GTATGTATGT TTTAATGGAG CTTATAGAAA CAGAAAAGAT GTATGTTGAA 
GACTTGCGCC AAATAGTGGA GGGTTACATG GCCACCATGA ATACACAAGG AATTCCAGAA 
GACATGAAGG GAAAAGACAA AATTGTTTTT GGGAATATAC ACCAGATCTT TGATTGGCAC 
AAAGACTATT TTTTAAGTGA GCTTCAAAAG TGCCTTGAGG ACCCAAACAG ATTAGCGATT 
CTCTTCATCA AGCATGAGCG CCGGCTGCAC ATGTATGTGG TGTACTGTCA GAACAAACCC 
AAGTCTGAGC ACATTGTATC AGAGTATATT GACACCTACT TTGAGGAGTT AAGACAACAG 
CTCTCACACC GCCTTCAACT CAACGACCTG CTTATCAAGC CAGTGCAGAG GATAATGAAG 
TATCAGTTGC TGCTAAAAGA CTTTCTTAAA TATTACAGCA AAGCTGGACA AGACACATCA 
GAACTGGAGA AAGCTGTGGA AGTTATGTGT TTCGTGCCCA AGCGCTGTAA TGACATGATG 
AATCTAGGCA GGCTTCAGGG ATTTGAGGGA AAAATTACCG CTCAGGGGAA GCTTCTGCAA 
CAAGACACAT TTCTGGTGAA CGAGCAGGAG AGCAGCTTTC TGACACGCTG CAAAGAACGC 
AGGGTCTTCC TCTTTGAACA AATAATCATC TTTAGTGAAC CGATTGATAA AAAGAAAGAT 
TTCTCCTTGC CTGGATACAT CTTTAAAAAT AGCATTAAGA TTAGTTGCCT TGGCATTGAA 
GAGTCTATAG ATAATGATCC TTGTAAGTTT GTCCTGACAT CACGAGGTGC TGATAGGAGC 
TTGGTCCGGT ACATACTGCA GGCCTCCACG CAAGAGATCC GCTTGGCTTG GATAAAAGAT 
ATTGGACAGA TCTTAGAGAG TCAGAGGAAC TTTCTCAATG CCCTTCAGTC TCCTATTGAA 
TACCAACGCC GAGAAAGCAA ATCCAATAGT CTGGGCAGAA ACAGTAACCT CCGCATTATG 
GAAACTTCCC GTGGAGGGTT AAGGCCCCAT TCTTCCTCTT CTATGGACAG GAACAAAGTT 
CCCAACCTCA AGTCATACAA TTCTTCCCTG CCTTCCCTTT ATTTGCCAAG CAACCCTCGA 
CAAGACAGTA GACAGTTTGG AAACAAGGTA CACTCAGCTC GAAAAAGTTC TCACTCAGAT 
GCCCTCCCTT CACCGGCAAT CCTTTCCCCA ACACACATCA GCCTTGACTT CCATCATCCA 
GAGTCCTCTT CACCGACAGT GTCCAATGGG ACCCCACAAA GTGACTCCAC CAGCCGGAAA 
CCAAGCCTAT CAGAACGTGA ACTACCTGGC TATGCATCGT CTCCTGAAGA TTATGGTGTT 
CCTACTCCCA GGTTGGCCAA GCTGGATGAA GATGAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811994.1
CDS	197..2458
Translation	MKAPQRSGGRTDQMLGAMADILGSFGLPSHQWLPDSAPEQSRNEEKTDGENSPDEEEEQPNVDPQHTASGLQVPTDSTCPGMGRTRRLSDFRAWFGENFFHTSEEESYSIAGSDGSISASVASIQPQPSTSSAPSSPGSRRSVSTLKKWLTNPVRKLSSGSRLDQRQSKKIESRPKRGVPGRRRSADDGGRRSFDLGVLRKMDEFTHPEDKGSEWKEQDHPERLPDLSILNDLLQGVTTEPNNKDPHTSYLSDPTDSLASELPVTPSFPPAETEEEKKSALEKSMYVLMELIETEKMYVEDLRQIVEGYMATMNTQGIPEDMKGKDKIVFGNIHQIFDWHKDYFLSELQKCLEDPNRLAILFIKHERRLHMYVVYCQNKPKSEHIVSEYIDTYFEELRQQLSHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYSKAGQDTSELEKAVEVMCFVPKRCNDMMNLGRLQGFEGKITAQGKLLQQDTFLVNEQESSFLTRCKERRVFLFEQIIIFSEPIDKKKDFSLPGYIFKNSIKISCLGIEESIDNDPCKFVLTSRGADRSLVRYILQASTQEIRLAWIKDIGQILESQRNFLNALQSPIEYQRRESKSNSLGRNSNLRIMETSRGGLRPHSSSSMDRNKVPNLKSYNSSLPSLYLPSNPRQDSRQFGNKVHSARKSSHSDALPSPAILSPTHISLDFHHPESSSPTVSNGTPQSDSTSRKPSLSERELPGYASSPEDYGVPTPRLAKLDEDEL

Target ORF information:

RefSeq Version	XM_012956540.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956540.3

ORF Insert Sequence:

ATGAAGGCAC CTCAGCGCTC AGGGGGGCGC ACTGATCAGA TGCTCGGCGC TATGGCAGAC 
ATCCTGGGAT CATTTGGACT TCCGAGCCAC CAGTGGCTTC CAGACTCAGC CCCTGAGCAA 
AGTAGGAACG AAGAGAAGAC CGACGGGGAG AATTCCCCAG ATGAAGAGGA AGAACAACCC 
AATGTGGACC CACAACATAC AGCATCTGGT CTCCAAGTTC CTACTGATAG CACATGCCCT 
GGGATGGGCA GAACCAGACG CCTCTCGGAC TTCCGTGCGT GGTTTGGAGA AAACTTCTTT 
CACACTTCAG AAGAAGAATC CTACTCAATT GCTGGCAGCG ATGGGAGCAT ATCAGCTTCG 
GTGGCTTCCA TCCAACCACA ACCTTCAACT AGTTCAGCAC CCAGTTCCCC TGGATCCAGG 
AGGTCAGTCA GCACCTTGAA AAAATGGCTG ACAAACCCAG TCAGGAAGCT GAGCAGTGGC 
AGCCGTCTAG ATCAACGACA GAGTAAGAAA ATTGAAAGCA GGCCAAAGAG AGGTGTACCA 
GGCCGGAGGA GGAGTGCAGA TGATGGAGGC AGAAGAAGTT TTGATCTAGG GGTCTTGCGG 
AAAATGGATG AGTTTACCCA TCCTGAGGAT AAGGGTTCGG AATGGAAGGA ACAGGATCAC 
CCTGAGCGGT TGCCTGACTT ATCAATATTA AATGACTTGC TACAAGGGGT AACTACAGAA 
CCCAACAATA AGGATCCGCA CACATCCTAC CTCTCCGATC CTACGGATTC TTTGGCATCA 
GAACTACCTG TTACTCCCTC CTTCCCCCCA GCAGAAACAG AAGAAGAAAA GAAGAGTGCC 
TTAGAAAAGA GTATGTATGT TTTAATGGAG CTTATAGAAA CAGAAAAGAT GTATGTTGAA 
GACTTGCGCC AAATAGTGGA GGGTTACATG GCCACCATGA ATACACAAGG AATTCCAGAA 
GACATGAAGG GAAAAGACAA AATTGTTTTT GGGAATATAC ACCAGATCTT TGATTGGCAC 
AAAGACTATT TTTTAAGTGA GCTTCAAAAG TGCCTTGAGG ACCCAAACAG ATTAGCGATT 
CTCTTCATCA AGCATGAGCG CCGGCTGCAC ATGTATGTGG TGTACTGTCA GAACAAACCC 
AAGTCTGAGC ACATTGTATC AGAGTATATT GACACCTACT TTGAGGAGTT AAGACAACAG 
CTCTCACACC GCCTTCAACT CAACGACCTG CTTATCAAGC CAGTGCAGAG GATAATGAAG 
TATCAGTTGC TGCTAAAAGA CTTTCTTAAA TATTACAGCA AAGCTGGACA AGACACATCA 
GAACTGGAGA AAGCTGTGGA AGTTATGTGT TTCGTGCCCA AGCGCTGTAA TGACATGATG 
AATCTAGGCA GGCTTCAGGG ATTTGAGGGA AAAATTACCG CTCAGGGGAA GCTTCTGCAA 
CAAGACACAT TTCTGGTGAA CGAGCAGGAG AGCAGCTTTC TGACACGCTG CAAAGAACGC 
AGGGTCTTCC TCTTTGAACA AATAATCATC TTTAGTGAAC CGATTGATAA AAAGAAAGAT 
TTCTCCTTGC CTGGATACAT CTTTAAAAAT AGCATTAAGA TTAGTTGCCT TGGCATTGAA 
GAGTCTATAG ATAATGATCC TTGTAAGTTT GTCCTGACAT CACGAGGTGC TGATAGGAGC 
TTGGTCCGGT ACATACTGCA GGCCTCCACG CAAGAGATCC GCTTGGCTTG GATAAAAGAT 
ATTGGACAGA TCTTAGAGAG TCAGAGGAAC TTTCTCAATG CCCTTCAGTC TCCTATTGAA 
TACCAACGCC GAGAAAGCAA ATCCAATAGT CTGGGCAGAA ACAGTAACCT CCGCATTATG 
GAAACTTCCC GTGGAGGGTT AAGGCCCCAT TCTTCCTCTT CTATGGACAG GAACAAAGTT 
CCCAACCTCA AGTCATACAA TTCTTCCCTG CCTTCCCTTT ATTTGCCAAG CAACCCTCGA 
CAAGACAGTA GACAGTTTGG AAACAAGGTA CACTCAGCTC GAAAAAGTTC TCACTCAGAT 
GCCCTCCCTT CACCGGCAAT CCTTTCCCCA ACACACATCA GCCTTGACTT CCATCATCCA 
GAGTCCTCTT CACCGACAGT GTCCAATGGG ACCCCACAAA GTGACTCCAC CAGCCGGAAA 
CCAAGCCTAT CAGAACGTGA ACTACCTGGC TATGCATCGT CTCCTGAAGA TTATGGTGTT 
CCTACTCCCA GGTTGGCCAA GCTGGATGAA GATGAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef25 ( XM_031895732.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751592.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X3, mRNA.

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CloneID	OXa32362
Clone ID Related Accession (Same CDS sequence)	XM_012956540.2 , XM_031895731.1 , XM_012956540.3 , XM_031895732.1 , XM_031895733.1
Accession Version	XM_031895732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC CTCAGCGCTC AGGGGGGCGC ACTGATCAGA TGCTCGGCGC TATGGCAGAC 
ATCCTGGGAT CATTTGGACT TCCGAGCCAC CAGTGGCTTC CAGACTCAGC CCCTGAGCAA 
AGTAGGAACG AAGAGAAGAC CGACGGGGAG AATTCCCCAG ATGAAGAGGA AGAACAACCC 
AATGTGGACC CACAACATAC AGCATCTGGT CTCCAAGTTC CTACTGATAG CACATGCCCT 
GGGATGGGCA GAACCAGACG CCTCTCGGAC TTCCGTGCGT GGTTTGGAGA AAACTTCTTT 
CACACTTCAG AAGAAGAATC CTACTCAATT GCTGGCAGCG ATGGGAGCAT ATCAGCTTCG 
GTGGCTTCCA TCCAACCACA ACCTTCAACT AGTTCAGCAC CCAGTTCCCC TGGATCCAGG 
AGGTCAGTCA GCACCTTGAA AAAATGGCTG ACAAACCCAG TCAGGAAGCT GAGCAGTGGC 
AGCCGTCTAG ATCAACGACA GAGTAAGAAA ATTGAAAGCA GGCCAAAGAG AGGTGTACCA 
GGCCGGAGGA GGAGTGCAGA TGATGGAGGC AGAAGAAGTT TTGATCTAGG GGTCTTGCGG 
AAAATGGATG AGTTTACCCA TCCTGAGGAT AAGGGTTCGG AATGGAAGGA ACAGGATCAC 
CCTGAGCGGT TGCCTGACTT ATCAATATTA AATGACTTGC TACAAGGGGT AACTACAGAA 
CCCAACAATA AGGATCCGCA CACATCCTAC CTCTCCGATC CTACGGATTC TTTGGCATCA 
GAACTACCTG TTACTCCCTC CTTCCCCCCA GCAGAAACAG AAGAAGAAAA GAAGAGTGCC 
TTAGAAAAGA GTATGTATGT TTTAATGGAG CTTATAGAAA CAGAAAAGAT GTATGTTGAA 
GACTTGCGCC AAATAGTGGA GGGTTACATG GCCACCATGA ATACACAAGG AATTCCAGAA 
GACATGAAGG GAAAAGACAA AATTGTTTTT GGGAATATAC ACCAGATCTT TGATTGGCAC 
AAAGACTATT TTTTAAGTGA GCTTCAAAAG TGCCTTGAGG ACCCAAACAG ATTAGCGATT 
CTCTTCATCA AGCATGAGCG CCGGCTGCAC ATGTATGTGG TGTACTGTCA GAACAAACCC 
AAGTCTGAGC ACATTGTATC AGAGTATATT GACACCTACT TTGAGGAGTT AAGACAACAG 
CTCTCACACC GCCTTCAACT CAACGACCTG CTTATCAAGC CAGTGCAGAG GATAATGAAG 
TATCAGTTGC TGCTAAAAGA CTTTCTTAAA TATTACAGCA AAGCTGGACA AGACACATCA 
GAACTGGAGA AAGCTGTGGA AGTTATGTGT TTCGTGCCCA AGCGCTGTAA TGACATGATG 
AATCTAGGCA GGCTTCAGGG ATTTGAGGGA AAAATTACCG CTCAGGGGAA GCTTCTGCAA 
CAAGACACAT TTCTGGTGAA CGAGCAGGAG AGCAGCTTTC TGACACGCTG CAAAGAACGC 
AGGGTCTTCC TCTTTGAACA AATAATCATC TTTAGTGAAC CGATTGATAA AAAGAAAGAT 
TTCTCCTTGC CTGGATACAT CTTTAAAAAT AGCATTAAGA TTAGTTGCCT TGGCATTGAA 
GAGTCTATAG ATAATGATCC TTGTAAGTTT GTCCTGACAT CACGAGGTGC TGATAGGAGC 
TTGGTCCGGT ACATACTGCA GGCCTCCACG CAAGAGATCC GCTTGGCTTG GATAAAAGAT 
ATTGGACAGA TCTTAGAGAG TCAGAGGAAC TTTCTCAATG CCCTTCAGTC TCCTATTGAA 
TACCAACGCC GAGAAAGCAA ATCCAATAGT CTGGGCAGAA ACAGTAACCT CCGCATTATG 
GAAACTTCCC GTGGAGGGTT AAGGCCCCAT TCTTCCTCTT CTATGGACAG GAACAAAGTT 
CCCAACCTCA AGTCATACAA TTCTTCCCTG CCTTCCCTTT ATTTGCCAAG CAACCCTCGA 
CAAGACAGTA GACAGTTTGG AAACAAGGTA CACTCAGCTC GAAAAAGTTC TCACTCAGAT 
GCCCTCCCTT CACCGGCAAT CCTTTCCCCA ACACACATCA GCCTTGACTT CCATCATCCA 
GAGTCCTCTT CACCGACAGT GTCCAATGGG ACCCCACAAA GTGACTCCAC CAGCCGGAAA 
CCAAGCCTAT CAGAACGTGA ACTACCTGGC TATGCATCGT CTCCTGAAGA TTATGGTGTT 
CCTACTCCCA GGTTGGCCAA GCTGGATGAA GATGAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751592.1
CDS	347..2608
Translation	MKAPQRSGGRTDQMLGAMADILGSFGLPSHQWLPDSAPEQSRNEEKTDGENSPDEEEEQPNVDPQHTASGLQVPTDSTCPGMGRTRRLSDFRAWFGENFFHTSEEESYSIAGSDGSISASVASIQPQPSTSSAPSSPGSRRSVSTLKKWLTNPVRKLSSGSRLDQRQSKKIESRPKRGVPGRRRSADDGGRRSFDLGVLRKMDEFTHPEDKGSEWKEQDHPERLPDLSILNDLLQGVTTEPNNKDPHTSYLSDPTDSLASELPVTPSFPPAETEEEKKSALEKSMYVLMELIETEKMYVEDLRQIVEGYMATMNTQGIPEDMKGKDKIVFGNIHQIFDWHKDYFLSELQKCLEDPNRLAILFIKHERRLHMYVVYCQNKPKSEHIVSEYIDTYFEELRQQLSHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYSKAGQDTSELEKAVEVMCFVPKRCNDMMNLGRLQGFEGKITAQGKLLQQDTFLVNEQESSFLTRCKERRVFLFEQIIIFSEPIDKKKDFSLPGYIFKNSIKISCLGIEESIDNDPCKFVLTSRGADRSLVRYILQASTQEIRLAWIKDIGQILESQRNFLNALQSPIEYQRRESKSNSLGRNSNLRIMETSRGGLRPHSSSSMDRNKVPNLKSYNSSLPSLYLPSNPRQDSRQFGNKVHSARKSSHSDALPSPAILSPTHISLDFHHPESSSPTVSNGTPQSDSTSRKPSLSERELPGYASSPEDYGVPTPRLAKLDEDEL

Target ORF information:

RefSeq Version	XM_031895732.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895732.1

ORF Insert Sequence:

ATGAAGGCAC CTCAGCGCTC AGGGGGGCGC ACTGATCAGA TGCTCGGCGC TATGGCAGAC 
ATCCTGGGAT CATTTGGACT TCCGAGCCAC CAGTGGCTTC CAGACTCAGC CCCTGAGCAA 
AGTAGGAACG AAGAGAAGAC CGACGGGGAG AATTCCCCAG ATGAAGAGGA AGAACAACCC 
AATGTGGACC CACAACATAC AGCATCTGGT CTCCAAGTTC CTACTGATAG CACATGCCCT 
GGGATGGGCA GAACCAGACG CCTCTCGGAC TTCCGTGCGT GGTTTGGAGA AAACTTCTTT 
CACACTTCAG AAGAAGAATC CTACTCAATT GCTGGCAGCG ATGGGAGCAT ATCAGCTTCG 
GTGGCTTCCA TCCAACCACA ACCTTCAACT AGTTCAGCAC CCAGTTCCCC TGGATCCAGG 
AGGTCAGTCA GCACCTTGAA AAAATGGCTG ACAAACCCAG TCAGGAAGCT GAGCAGTGGC 
AGCCGTCTAG ATCAACGACA GAGTAAGAAA ATTGAAAGCA GGCCAAAGAG AGGTGTACCA 
GGCCGGAGGA GGAGTGCAGA TGATGGAGGC AGAAGAAGTT TTGATCTAGG GGTCTTGCGG 
AAAATGGATG AGTTTACCCA TCCTGAGGAT AAGGGTTCGG AATGGAAGGA ACAGGATCAC 
CCTGAGCGGT TGCCTGACTT ATCAATATTA AATGACTTGC TACAAGGGGT AACTACAGAA 
CCCAACAATA AGGATCCGCA CACATCCTAC CTCTCCGATC CTACGGATTC TTTGGCATCA 
GAACTACCTG TTACTCCCTC CTTCCCCCCA GCAGAAACAG AAGAAGAAAA GAAGAGTGCC 
TTAGAAAAGA GTATGTATGT TTTAATGGAG CTTATAGAAA CAGAAAAGAT GTATGTTGAA 
GACTTGCGCC AAATAGTGGA GGGTTACATG GCCACCATGA ATACACAAGG AATTCCAGAA 
GACATGAAGG GAAAAGACAA AATTGTTTTT GGGAATATAC ACCAGATCTT TGATTGGCAC 
AAAGACTATT TTTTAAGTGA GCTTCAAAAG TGCCTTGAGG ACCCAAACAG ATTAGCGATT 
CTCTTCATCA AGCATGAGCG CCGGCTGCAC ATGTATGTGG TGTACTGTCA GAACAAACCC 
AAGTCTGAGC ACATTGTATC AGAGTATATT GACACCTACT TTGAGGAGTT AAGACAACAG 
CTCTCACACC GCCTTCAACT CAACGACCTG CTTATCAAGC CAGTGCAGAG GATAATGAAG 
TATCAGTTGC TGCTAAAAGA CTTTCTTAAA TATTACAGCA AAGCTGGACA AGACACATCA 
GAACTGGAGA AAGCTGTGGA AGTTATGTGT TTCGTGCCCA AGCGCTGTAA TGACATGATG 
AATCTAGGCA GGCTTCAGGG ATTTGAGGGA AAAATTACCG CTCAGGGGAA GCTTCTGCAA 
CAAGACACAT TTCTGGTGAA CGAGCAGGAG AGCAGCTTTC TGACACGCTG CAAAGAACGC 
AGGGTCTTCC TCTTTGAACA AATAATCATC TTTAGTGAAC CGATTGATAA AAAGAAAGAT 
TTCTCCTTGC CTGGATACAT CTTTAAAAAT AGCATTAAGA TTAGTTGCCT TGGCATTGAA 
GAGTCTATAG ATAATGATCC TTGTAAGTTT GTCCTGACAT CACGAGGTGC TGATAGGAGC 
TTGGTCCGGT ACATACTGCA GGCCTCCACG CAAGAGATCC GCTTGGCTTG GATAAAAGAT 
ATTGGACAGA TCTTAGAGAG TCAGAGGAAC TTTCTCAATG CCCTTCAGTC TCCTATTGAA 
TACCAACGCC GAGAAAGCAA ATCCAATAGT CTGGGCAGAA ACAGTAACCT CCGCATTATG 
GAAACTTCCC GTGGAGGGTT AAGGCCCCAT TCTTCCTCTT CTATGGACAG GAACAAAGTT 
CCCAACCTCA AGTCATACAA TTCTTCCCTG CCTTCCCTTT ATTTGCCAAG CAACCCTCGA 
CAAGACAGTA GACAGTTTGG AAACAAGGTA CACTCAGCTC GAAAAAGTTC TCACTCAGAT 
GCCCTCCCTT CACCGGCAAT CCTTTCCCCA ACACACATCA GCCTTGACTT CCATCATCCA 
GAGTCCTCTT CACCGACAGT GTCCAATGGG ACCCCACAAA GTGACTCCAC CAGCCGGAAA 
CCAAGCCTAT CAGAACGTGA ACTACCTGGC TATGCATCGT CTCCTGAAGA TTATGGTGTT 
CCTACTCCCA GGTTGGCCAA GCTGGATGAA GATGAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef25 ( XM_031895733.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751593.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa32362
Clone ID Related Accession (Same CDS sequence)	XM_012956540.2 , XM_031895731.1 , XM_012956540.3 , XM_031895732.1 , XM_031895733.1
Accession Version	XM_031895733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC CTCAGCGCTC AGGGGGGCGC ACTGATCAGA TGCTCGGCGC TATGGCAGAC 
ATCCTGGGAT CATTTGGACT TCCGAGCCAC CAGTGGCTTC CAGACTCAGC CCCTGAGCAA 
AGTAGGAACG AAGAGAAGAC CGACGGGGAG AATTCCCCAG ATGAAGAGGA AGAACAACCC 
AATGTGGACC CACAACATAC AGCATCTGGT CTCCAAGTTC CTACTGATAG CACATGCCCT 
GGGATGGGCA GAACCAGACG CCTCTCGGAC TTCCGTGCGT GGTTTGGAGA AAACTTCTTT 
CACACTTCAG AAGAAGAATC CTACTCAATT GCTGGCAGCG ATGGGAGCAT ATCAGCTTCG 
GTGGCTTCCA TCCAACCACA ACCTTCAACT AGTTCAGCAC CCAGTTCCCC TGGATCCAGG 
AGGTCAGTCA GCACCTTGAA AAAATGGCTG ACAAACCCAG TCAGGAAGCT GAGCAGTGGC 
AGCCGTCTAG ATCAACGACA GAGTAAGAAA ATTGAAAGCA GGCCAAAGAG AGGTGTACCA 
GGCCGGAGGA GGAGTGCAGA TGATGGAGGC AGAAGAAGTT TTGATCTAGG GGTCTTGCGG 
AAAATGGATG AGTTTACCCA TCCTGAGGAT AAGGGTTCGG AATGGAAGGA ACAGGATCAC 
CCTGAGCGGT TGCCTGACTT ATCAATATTA AATGACTTGC TACAAGGGGT AACTACAGAA 
CCCAACAATA AGGATCCGCA CACATCCTAC CTCTCCGATC CTACGGATTC TTTGGCATCA 
GAACTACCTG TTACTCCCTC CTTCCCCCCA GCAGAAACAG AAGAAGAAAA GAAGAGTGCC 
TTAGAAAAGA GTATGTATGT TTTAATGGAG CTTATAGAAA CAGAAAAGAT GTATGTTGAA 
GACTTGCGCC AAATAGTGGA GGGTTACATG GCCACCATGA ATACACAAGG AATTCCAGAA 
GACATGAAGG GAAAAGACAA AATTGTTTTT GGGAATATAC ACCAGATCTT TGATTGGCAC 
AAAGACTATT TTTTAAGTGA GCTTCAAAAG TGCCTTGAGG ACCCAAACAG ATTAGCGATT 
CTCTTCATCA AGCATGAGCG CCGGCTGCAC ATGTATGTGG TGTACTGTCA GAACAAACCC 
AAGTCTGAGC ACATTGTATC AGAGTATATT GACACCTACT TTGAGGAGTT AAGACAACAG 
CTCTCACACC GCCTTCAACT CAACGACCTG CTTATCAAGC CAGTGCAGAG GATAATGAAG 
TATCAGTTGC TGCTAAAAGA CTTTCTTAAA TATTACAGCA AAGCTGGACA AGACACATCA 
GAACTGGAGA AAGCTGTGGA AGTTATGTGT TTCGTGCCCA AGCGCTGTAA TGACATGATG 
AATCTAGGCA GGCTTCAGGG ATTTGAGGGA AAAATTACCG CTCAGGGGAA GCTTCTGCAA 
CAAGACACAT TTCTGGTGAA CGAGCAGGAG AGCAGCTTTC TGACACGCTG CAAAGAACGC 
AGGGTCTTCC TCTTTGAACA AATAATCATC TTTAGTGAAC CGATTGATAA AAAGAAAGAT 
TTCTCCTTGC CTGGATACAT CTTTAAAAAT AGCATTAAGA TTAGTTGCCT TGGCATTGAA 
GAGTCTATAG ATAATGATCC TTGTAAGTTT GTCCTGACAT CACGAGGTGC TGATAGGAGC 
TTGGTCCGGT ACATACTGCA GGCCTCCACG CAAGAGATCC GCTTGGCTTG GATAAAAGAT 
ATTGGACAGA TCTTAGAGAG TCAGAGGAAC TTTCTCAATG CCCTTCAGTC TCCTATTGAA 
TACCAACGCC GAGAAAGCAA ATCCAATAGT CTGGGCAGAA ACAGTAACCT CCGCATTATG 
GAAACTTCCC GTGGAGGGTT AAGGCCCCAT TCTTCCTCTT CTATGGACAG GAACAAAGTT 
CCCAACCTCA AGTCATACAA TTCTTCCCTG CCTTCCCTTT ATTTGCCAAG CAACCCTCGA 
CAAGACAGTA GACAGTTTGG AAACAAGGTA CACTCAGCTC GAAAAAGTTC TCACTCAGAT 
GCCCTCCCTT CACCGGCAAT CCTTTCCCCA ACACACATCA GCCTTGACTT CCATCATCCA 
GAGTCCTCTT CACCGACAGT GTCCAATGGG ACCCCACAAA GTGACTCCAC CAGCCGGAAA 
CCAAGCCTAT CAGAACGTGA ACTACCTGGC TATGCATCGT CTCCTGAAGA TTATGGTGTT 
CCTACTCCCA GGTTGGCCAA GCTGGATGAA GATGAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751593.1
CDS	279..2540
Translation	MKAPQRSGGRTDQMLGAMADILGSFGLPSHQWLPDSAPEQSRNEEKTDGENSPDEEEEQPNVDPQHTASGLQVPTDSTCPGMGRTRRLSDFRAWFGENFFHTSEEESYSIAGSDGSISASVASIQPQPSTSSAPSSPGSRRSVSTLKKWLTNPVRKLSSGSRLDQRQSKKIESRPKRGVPGRRRSADDGGRRSFDLGVLRKMDEFTHPEDKGSEWKEQDHPERLPDLSILNDLLQGVTTEPNNKDPHTSYLSDPTDSLASELPVTPSFPPAETEEEKKSALEKSMYVLMELIETEKMYVEDLRQIVEGYMATMNTQGIPEDMKGKDKIVFGNIHQIFDWHKDYFLSELQKCLEDPNRLAILFIKHERRLHMYVVYCQNKPKSEHIVSEYIDTYFEELRQQLSHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYSKAGQDTSELEKAVEVMCFVPKRCNDMMNLGRLQGFEGKITAQGKLLQQDTFLVNEQESSFLTRCKERRVFLFEQIIIFSEPIDKKKDFSLPGYIFKNSIKISCLGIEESIDNDPCKFVLTSRGADRSLVRYILQASTQEIRLAWIKDIGQILESQRNFLNALQSPIEYQRRESKSNSLGRNSNLRIMETSRGGLRPHSSSSMDRNKVPNLKSYNSSLPSLYLPSNPRQDSRQFGNKVHSARKSSHSDALPSPAILSPTHISLDFHHPESSSPTVSNGTPQSDSTSRKPSLSERELPGYASSPEDYGVPTPRLAKLDEDEL

Target ORF information:

RefSeq Version	XM_031895733.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895733.1

ORF Insert Sequence:

ATGAAGGCAC CTCAGCGCTC AGGGGGGCGC ACTGATCAGA TGCTCGGCGC TATGGCAGAC 
ATCCTGGGAT CATTTGGACT TCCGAGCCAC CAGTGGCTTC CAGACTCAGC CCCTGAGCAA 
AGTAGGAACG AAGAGAAGAC CGACGGGGAG AATTCCCCAG ATGAAGAGGA AGAACAACCC 
AATGTGGACC CACAACATAC AGCATCTGGT CTCCAAGTTC CTACTGATAG CACATGCCCT 
GGGATGGGCA GAACCAGACG CCTCTCGGAC TTCCGTGCGT GGTTTGGAGA AAACTTCTTT 
CACACTTCAG AAGAAGAATC CTACTCAATT GCTGGCAGCG ATGGGAGCAT ATCAGCTTCG 
GTGGCTTCCA TCCAACCACA ACCTTCAACT AGTTCAGCAC CCAGTTCCCC TGGATCCAGG 
AGGTCAGTCA GCACCTTGAA AAAATGGCTG ACAAACCCAG TCAGGAAGCT GAGCAGTGGC 
AGCCGTCTAG ATCAACGACA GAGTAAGAAA ATTGAAAGCA GGCCAAAGAG AGGTGTACCA 
GGCCGGAGGA GGAGTGCAGA TGATGGAGGC AGAAGAAGTT TTGATCTAGG GGTCTTGCGG 
AAAATGGATG AGTTTACCCA TCCTGAGGAT AAGGGTTCGG AATGGAAGGA ACAGGATCAC 
CCTGAGCGGT TGCCTGACTT ATCAATATTA AATGACTTGC TACAAGGGGT AACTACAGAA 
CCCAACAATA AGGATCCGCA CACATCCTAC CTCTCCGATC CTACGGATTC TTTGGCATCA 
GAACTACCTG TTACTCCCTC CTTCCCCCCA GCAGAAACAG AAGAAGAAAA GAAGAGTGCC 
TTAGAAAAGA GTATGTATGT TTTAATGGAG CTTATAGAAA CAGAAAAGAT GTATGTTGAA 
GACTTGCGCC AAATAGTGGA GGGTTACATG GCCACCATGA ATACACAAGG AATTCCAGAA 
GACATGAAGG GAAAAGACAA AATTGTTTTT GGGAATATAC ACCAGATCTT TGATTGGCAC 
AAAGACTATT TTTTAAGTGA GCTTCAAAAG TGCCTTGAGG ACCCAAACAG ATTAGCGATT 
CTCTTCATCA AGCATGAGCG CCGGCTGCAC ATGTATGTGG TGTACTGTCA GAACAAACCC 
AAGTCTGAGC ACATTGTATC AGAGTATATT GACACCTACT TTGAGGAGTT AAGACAACAG 
CTCTCACACC GCCTTCAACT CAACGACCTG CTTATCAAGC CAGTGCAGAG GATAATGAAG 
TATCAGTTGC TGCTAAAAGA CTTTCTTAAA TATTACAGCA AAGCTGGACA AGACACATCA 
GAACTGGAGA AAGCTGTGGA AGTTATGTGT TTCGTGCCCA AGCGCTGTAA TGACATGATG 
AATCTAGGCA GGCTTCAGGG ATTTGAGGGA AAAATTACCG CTCAGGGGAA GCTTCTGCAA 
CAAGACACAT TTCTGGTGAA CGAGCAGGAG AGCAGCTTTC TGACACGCTG CAAAGAACGC 
AGGGTCTTCC TCTTTGAACA AATAATCATC TTTAGTGAAC CGATTGATAA AAAGAAAGAT 
TTCTCCTTGC CTGGATACAT CTTTAAAAAT AGCATTAAGA TTAGTTGCCT TGGCATTGAA 
GAGTCTATAG ATAATGATCC TTGTAAGTTT GTCCTGACAT CACGAGGTGC TGATAGGAGC 
TTGGTCCGGT ACATACTGCA GGCCTCCACG CAAGAGATCC GCTTGGCTTG GATAAAAGAT 
ATTGGACAGA TCTTAGAGAG TCAGAGGAAC TTTCTCAATG CCCTTCAGTC TCCTATTGAA 
TACCAACGCC GAGAAAGCAA ATCCAATAGT CTGGGCAGAA ACAGTAACCT CCGCATTATG 
GAAACTTCCC GTGGAGGGTT AAGGCCCCAT TCTTCCTCTT CTATGGACAG GAACAAAGTT 
CCCAACCTCA AGTCATACAA TTCTTCCCTG CCTTCCCTTT ATTTGCCAAG CAACCCTCGA 
CAAGACAGTA GACAGTTTGG AAACAAGGTA CACTCAGCTC GAAAAAGTTC TCACTCAGAT 
GCCCTCCCTT CACCGGCAAT CCTTTCCCCA ACACACATCA GCCTTGACTT CCATCATCCA 
GAGTCCTCTT CACCGACAGT GTCCAATGGG ACCCCACAAA GTGACTCCAC CAGCCGGAAA 
CCAAGCCTAT CAGAACGTGA ACTACCTGGC TATGCATCGT CTCCTGAAGA TTATGGTGTT 
CCTACTCCCA GGTTGGCCAA GCTGGATGAA GATGAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef25 ( XM_018091106.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946595.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa42463
Clone ID Related Accession (Same CDS sequence)	XM_018091106.1
Accession Version	XM_018091106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAC CTCAGCGCTC AGGGGGGCGC ACTGATCAGA TGCTCGGCGC TATGGCAGAC 
ATCCTGGGAT CATTTGGACT TCCGAGCCAC CAGTGGCTTC CAGACTCAGC CCCTGAGCAA 
AGTAGGAACG AAGAGAAGAC CGACGGGGAG AATTCCCCAG ATGAAGAGGA AGAACAACCC 
AATGTGGACC CACAACATAC AGCATCTGGT CTCCAAGTTC CTACTGATAG CACATGCCCT 
GGGATGGGCA GAACCAGACG CCTCTCGGAC TTCCGTGCGT GGTTTGGAGA AAACTTCTTT 
CACACTTCAG AAGAAGAATC CTACTCAATT GCTGGCAGCG ATGGGAGCAT ATCAGCTTCG 
GTGGCTTCCA TCCAACCACA ACCTTCAACT AGTTCAGCAC CCAGTTCCCC TGGATCCAGG 
AGGTCAGTCA GCACCTTGAA AAAATGGCTG ACAAACCCAG TCAGGAAGCT GAGCAGTGGC 
AGCCGTCTAG ATCAACGACA GAGTAAGAAA ATTGAAAGCA GGCCAAAGAG AGGTGTACCA 
GGCCGGAGGA GGAGTGCAGA TGATGGAGGC AGAAGAAGTT TTGATCTAGG GGTCTTGCGG 
AAAATGGATG AGTTTACCCA TCCTGAGGAT AAGGGTTCGG AATGGAAGGA ACAGGATCAC 
CCTGAGCGGT TGCCTGACTT ATCAATATTA AATGACTTGC TACAAGGGGT AACTACAGAA 
CCCAACAATA AGGATCCGCA CACATCCTAC CTCTCCGATC CTACGGATTC TTTGGCATCA 
GAACTACCTG TTACTCCCTC CTTCCCCCCA GCAGAAACAG AAGAAGAAAA GAAGAGTGCC 
TTAGAAAAGA GTATGTATGT TTTAATGGAG CTTATAGAAA CAGAAAAGAT GTATGTTGAA 
GACTTGCGCC AAATAGTGGA GGGTTACATG GCCACCATGA ATACACAAGG AATTCCAGAA 
GACATGAAGG GAAAAGACAA AATTGTTTTT GGGAATATAC ACCAGATCTT TGATTGGCAC 
AAAGACTATT TTTTAAGTGA GCTTCAAAAG TGCCTTGAGG ACCCAAACAG ATTAGCGATT 
CTCTTCATCA AGCATGAGTT AAGACAACAG CTCTCACACC GCCTTCAACT CAACGACCTG 
CTTATCAAGC CAGTGCAGAG GATAATGAAG TATCAGTTGC TGCTAAAAGA CTTTCTTAAA 
TATTACAGCA AAGCTGGACA AGACACATCA GAACTGGAGA AAGCTGTGGA AGTTATGTGT 
TTCGTGCCCA AGCGCTGTAA TGACATGATG AATCTAGGCA GGCTTCAGGG ATTTGAGGGA 
AAAATTACCG CTCAGGGGAA GCTTCTGCAA CAAGACACAT TTCTGGTGAA CGAGCAGGAG 
AGCAGCTTTC TGACACGCTG CAAAGAACGC AGGGTCTTCC TCTTTGAACA AATAATCATC 
TTTAGTGAAC CGATTGATAA AAAGAAAGAT TTCTCCTTGC CTGGATACAT CTTTAAAAAT 
AGCATTAAGA TTAGTTGCCT TGGCATTGAA GAGTCTATAG ATAATGATCC TTGTAAGTTT 
GTCCTGACAT CACGAGGTGC TGATAGGAGC TTGGTCCGGT ACATACTGCA GGCCTCCACG 
CAAGAGATCC GCTTGGCTTG GATAAAAGAT ATTGGACAGA TCTTAGAGAG TCAGAGGAAC 
TTTCTCAATG CCCTTCAGTC TCCTATTGAA TACCAACGCC GAGAAAGCAA ATCCAATAGT 
CTGGGCAGAA ACAGTAACCT CCGCATTATG GAAACTTCCC GTGGAGGGTT AAGGCCCCAT 
TCTTCCTCTT CTATGGACAG GAACAAAGTT CCCAACCTCA AGTCATACAA TTCTTCCCTG 
CCTTCCCTTT ATTTGCCAAG CAACCCTCGA CAAGACAGTA GACAGTTTGG AAACAAGGTA 
CACTCAGCTC GAAAAAGTTC TCACTCAGAT GCCCTCCCTT CACCGGCAAT CCTTTCCCCA 
ACACACATCA GCCTTGACTT CCATCATCCA GAGTCCTCTT CACCGACAGT GTCCAATGGG 
ACCCCACAAA GTGACTCCAC CAGCCGGAAA CCAAGCCTAT CAGAACGTGA ACTACCTGGC 
TATGCATCGT CTCCTGAAGA TTATGGTGTT CCTACTCCCA GGTTGGCCAA GCTGGATGAA 
GATGAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946595.1
CDS	597..2768
Translation	MKAPQRSGGRTDQMLGAMADILGSFGLPSHQWLPDSAPEQSRNEEKTDGENSPDEEEEQPNVDPQHTASGLQVPTDSTCPGMGRTRRLSDFRAWFGENFFHTSEEESYSIAGSDGSISASVASIQPQPSTSSAPSSPGSRRSVSTLKKWLTNPVRKLSSGSRLDQRQSKKIESRPKRGVPGRRRSADDGGRRSFDLGVLRKMDEFTHPEDKGSEWKEQDHPERLPDLSILNDLLQGVTTEPNNKDPHTSYLSDPTDSLASELPVTPSFPPAETEEEKKSALEKSMYVLMELIETEKMYVEDLRQIVEGYMATMNTQGIPEDMKGKDKIVFGNIHQIFDWHKDYFLSELQKCLEDPNRLAILFIKHELRQQLSHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYSKAGQDTSELEKAVEVMCFVPKRCNDMMNLGRLQGFEGKITAQGKLLQQDTFLVNEQESSFLTRCKERRVFLFEQIIIFSEPIDKKKDFSLPGYIFKNSIKISCLGIEESIDNDPCKFVLTSRGADRSLVRYILQASTQEIRLAWIKDIGQILESQRNFLNALQSPIEYQRRESKSNSLGRNSNLRIMETSRGGLRPHSSSSMDRNKVPNLKSYNSSLPSLYLPSNPRQDSRQFGNKVHSARKSSHSDALPSPAILSPTHISLDFHHPESSSPTVSNGTPQSDSTSRKPSLSERELPGYASSPEDYGVPTPRLAKLDEDEL

Target ORF information:

RefSeq Version	XM_018091106.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 25 (arhgef25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091106.1

ORF Insert Sequence:

ATGAAGGCAC CTCAGCGCTC AGGGGGGCGC ACTGATCAGA TGCTCGGCGC TATGGCAGAC 
ATCCTGGGAT CATTTGGACT TCCGAGCCAC CAGTGGCTTC CAGACTCAGC CCCTGAGCAA 
AGTAGGAACG AAGAGAAGAC CGACGGGGAG AATTCCCCAG ATGAAGAGGA AGAACAACCC 
AATGTGGACC CACAACATAC AGCATCTGGT CTCCAAGTTC CTACTGATAG CACATGCCCT 
GGGATGGGCA GAACCAGACG CCTCTCGGAC TTCCGTGCGT GGTTTGGAGA AAACTTCTTT 
CACACTTCAG AAGAAGAATC CTACTCAATT GCTGGCAGCG ATGGGAGCAT ATCAGCTTCG 
GTGGCTTCCA TCCAACCACA ACCTTCAACT AGTTCAGCAC CCAGTTCCCC TGGATCCAGG 
AGGTCAGTCA GCACCTTGAA AAAATGGCTG ACAAACCCAG TCAGGAAGCT GAGCAGTGGC 
AGCCGTCTAG ATCAACGACA GAGTAAGAAA ATTGAAAGCA GGCCAAAGAG AGGTGTACCA 
GGCCGGAGGA GGAGTGCAGA TGATGGAGGC AGAAGAAGTT TTGATCTAGG GGTCTTGCGG 
AAAATGGATG AGTTTACCCA TCCTGAGGAT AAGGGTTCGG AATGGAAGGA ACAGGATCAC 
CCTGAGCGGT TGCCTGACTT ATCAATATTA AATGACTTGC TACAAGGGGT AACTACAGAA 
CCCAACAATA AGGATCCGCA CACATCCTAC CTCTCCGATC CTACGGATTC TTTGGCATCA 
GAACTACCTG TTACTCCCTC CTTCCCCCCA GCAGAAACAG AAGAAGAAAA GAAGAGTGCC 
TTAGAAAAGA GTATGTATGT TTTAATGGAG CTTATAGAAA CAGAAAAGAT GTATGTTGAA 
GACTTGCGCC AAATAGTGGA GGGTTACATG GCCACCATGA ATACACAAGG AATTCCAGAA 
GACATGAAGG GAAAAGACAA AATTGTTTTT GGGAATATAC ACCAGATCTT TGATTGGCAC 
AAAGACTATT TTTTAAGTGA GCTTCAAAAG TGCCTTGAGG ACCCAAACAG ATTAGCGATT 
CTCTTCATCA AGCATGAGTT AAGACAACAG CTCTCACACC GCCTTCAACT CAACGACCTG 
CTTATCAAGC CAGTGCAGAG GATAATGAAG TATCAGTTGC TGCTAAAAGA CTTTCTTAAA 
TATTACAGCA AAGCTGGACA AGACACATCA GAACTGGAGA AAGCTGTGGA AGTTATGTGT 
TTCGTGCCCA AGCGCTGTAA TGACATGATG AATCTAGGCA GGCTTCAGGG ATTTGAGGGA 
AAAATTACCG CTCAGGGGAA GCTTCTGCAA CAAGACACAT TTCTGGTGAA CGAGCAGGAG 
AGCAGCTTTC TGACACGCTG CAAAGAACGC AGGGTCTTCC TCTTTGAACA AATAATCATC 
TTTAGTGAAC CGATTGATAA AAAGAAAGAT TTCTCCTTGC CTGGATACAT CTTTAAAAAT 
AGCATTAAGA TTAGTTGCCT TGGCATTGAA GAGTCTATAG ATAATGATCC TTGTAAGTTT 
GTCCTGACAT CACGAGGTGC TGATAGGAGC TTGGTCCGGT ACATACTGCA GGCCTCCACG 
CAAGAGATCC GCTTGGCTTG GATAAAAGAT ATTGGACAGA TCTTAGAGAG TCAGAGGAAC 
TTTCTCAATG CCCTTCAGTC TCCTATTGAA TACCAACGCC GAGAAAGCAA ATCCAATAGT 
CTGGGCAGAA ACAGTAACCT CCGCATTATG GAAACTTCCC GTGGAGGGTT AAGGCCCCAT 
TCTTCCTCTT CTATGGACAG GAACAAAGTT CCCAACCTCA AGTCATACAA TTCTTCCCTG 
CCTTCCCTTT ATTTGCCAAG CAACCCTCGA CAAGACAGTA GACAGTTTGG AAACAAGGTA 
CACTCAGCTC GAAAAAGTTC TCACTCAGAT GCCCTCCCTT CACCGGCAAT CCTTTCCCCA 
ACACACATCA GCCTTGACTT CCATCATCCA GAGTCCTCTT CACCGACAGT GTCCAATGGG 
ACCCCACAAA GTGACTCCAC CAGCCGGAAA CCAAGCCTAT CAGAACGTGA ACTACCTGGC 
TATGCATCGT CTCCTGAAGA TTATGGTGTT CCTACTCCCA GGTTGGCCAA GCTGGATGAA 
GATGAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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arhgef25 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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