neo1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following neo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the neo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa24188 NM_001129940.1
Latest version!
Xenopus tropicalis neogenin 1 (neo1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa32301 NM_001374564.1
Latest version!
Xenopus tropicalis neogenin 1 (neo1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32299 XM_012966143.2
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32300 XM_012966144.2
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32301 XM_012966145.2
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32302 XM_012966146.2
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32303 XM_012966147.2
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32304 XM_012966148.2
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32305 XM_012966149.2
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32306 XM_012966150.2
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa32307 XM_012966151.2
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa43037 XM_018091948.1
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53315 XM_012966143.3
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53316 XM_012966144.3
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53317 XM_012966146.3
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53318 XM_012966147.3
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53319 XM_012966148.3
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53320 XM_012966149.3
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53321 XM_012966150.3
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa53322 XM_012966151.3
Latest version!
Xenopus tropicalis neogenin 1 (neo1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa24188
    Clone ID Related Accession (Same CDS sequence) NM_001129940.1
    Accession Version NM_001129940.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2544bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-28
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167600.1. ##Evidence-Data-START## Transcript exon combination :: BC167600.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1025683, SAMEA1025684 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGTGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGGTAAGTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123412.1
    CDS130..2673
    Translation

    Target ORF information:

    RefSeq Version NM_001129940.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001129940.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGTGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGGTAAGTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32301
    Clone ID Related Accession (Same CDS sequence) XM_012966145.2 , NM_001374564.1
    Accession Version NM_001374564.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-03
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03012089.1 and AAMC03012090.1. On Oct 4, 2019 this sequence version replaced XM_012966145.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1025683, SAMEA1025684 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA
    GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA
    AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT
    TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC
    ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG
    AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC
    CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG
    ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA
    AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC
    TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT
    CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC
    TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG
    CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT
    GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA
    CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA
    GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT
    CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC
    AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC
    AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT
    CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361493.1
    CDS130..4437
    Translation

    Target ORF information:

    RefSeq Version NM_001374564.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001374564.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA
    GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA
    AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT
    TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC
    ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG
    AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC
    CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG
    ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA
    AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC
    TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT
    CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC
    TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG
    CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT
    GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA
    CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA
    GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT
    CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC
    AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC
    AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT
    CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32299
    Clone ID Related Accession (Same CDS sequence) XM_012966143.2
    Accession Version XM_012966143.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT
    GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG
    TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT
    AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC
    CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC
    ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC
    TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA
    TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC
    CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG
    GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC
    CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821597.1
    CDS236..4591
    Translation

    Target ORF information:

    RefSeq Version XM_012966143.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966143.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT
    GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG
    TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT
    AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC
    CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC
    ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC
    TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA
    TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC
    CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG
    GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC
    CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32300
    Clone ID Related Accession (Same CDS sequence) XM_012966144.2
    Accession Version XM_012966144.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGC ATCCAGCATC TCTGGGAAAG GAGTTCGCCC TTCTGATCCT
    GGATCAAATG GTGGCTCAAG TCTTGGAGGC AGTAATAGTC ACCATGGGAA CCCAACGTCA
    CTGGATCAGA ACATGCTTTT AACAATTATT GTTTCCATTG GTGTGATCAC TGTCTTGATT
    GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC CGTTCTAGCT CCCACCACAA AAAAAAGAGA
    GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT AAATACAAAG GCAACTCCAA GGATGTGAAA
    CCACCAGACC TGTGGATCCA TCATGAACGT TTGGAATTGA AACCAACAGA CAAGTCCCCA
    GAAACAAATC CTATTTTGAC AGACACGCCT CTGCCACGCT CCCCACAAGA TCTCACACCA
    GGGGATAGCA CCACGGAAAG CAGCATCCAC CAGCACAGCA ATTCGTATCG AGGTCTGGAA
    TCAGAAGATT CTGTGTCCTC ACTTGCTGGG CGCCGTGGCA TGCGACCTAA GATAATGATG
    CCTTTTGACT CCCAGCCTCC ACAACCGGTT GTCAGTGCCC ATCCCATCCA TTCCCACGAT
    AATTCTCATC ATTTTCGCTC CGTTCCTGGG TTCATGCTGC ACGTGTTGGG TGGCCCTCCA
    CAGGCTGCAG AATCTGTGCG TCATACCCCT AGCTGTGACA CTGTAGTAGC TGCATCATCT
    TCCTCTCAGA CTGGCCCTGA AGATCCAGAC CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC
    CTGGATGAAG ATTCAGTACC TAGTGTTCCC ACTGCACATA TCCGTCCTTC TCATCCCCTA
    AAAAGCTTTG CTGTTCCAGC TGTGCCCACC TCTGCTTCTG GAGCTTCGTA TGAACCCTCA
    TTACCTAGCT CTCCGCTTCT TCAACAACAA TGCCCCACCG CTCTGTCTGT AAAGACAGCC
    TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC CCTCTTCCAG TCACAGTTCC CAGTGCCCCT
    GAACCACTAG AGACAAGCAG GATGCTAGAG GGCTCAGAGT GTAGCTATGA ACCGGACGAG
    TTAAGCAAAG AGATGGCTCA CCTTGAAGGC CTGATGAAGG ATCTAAATGC CATTACCACA
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821598.1
    CDS236..4561
    Translation

    Target ORF information:

    RefSeq Version XM_012966144.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966144.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGC ATCCAGCATC TCTGGGAAAG GAGTTCGCCC TTCTGATCCT
    GGATCAAATG GTGGCTCAAG TCTTGGAGGC AGTAATAGTC ACCATGGGAA CCCAACGTCA
    CTGGATCAGA ACATGCTTTT AACAATTATT GTTTCCATTG GTGTGATCAC TGTCTTGATT
    GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC CGTTCTAGCT CCCACCACAA AAAAAAGAGA
    GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT AAATACAAAG GCAACTCCAA GGATGTGAAA
    CCACCAGACC TGTGGATCCA TCATGAACGT TTGGAATTGA AACCAACAGA CAAGTCCCCA
    GAAACAAATC CTATTTTGAC AGACACGCCT CTGCCACGCT CCCCACAAGA TCTCACACCA
    GGGGATAGCA CCACGGAAAG CAGCATCCAC CAGCACAGCA ATTCGTATCG AGGTCTGGAA
    TCAGAAGATT CTGTGTCCTC ACTTGCTGGG CGCCGTGGCA TGCGACCTAA GATAATGATG
    CCTTTTGACT CCCAGCCTCC ACAACCGGTT GTCAGTGCCC ATCCCATCCA TTCCCACGAT
    AATTCTCATC ATTTTCGCTC CGTTCCTGGG TTCATGCTGC ACGTGTTGGG TGGCCCTCCA
    CAGGCTGCAG AATCTGTGCG TCATACCCCT AGCTGTGACA CTGTAGTAGC TGCATCATCT
    TCCTCTCAGA CTGGCCCTGA AGATCCAGAC CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC
    CTGGATGAAG ATTCAGTACC TAGTGTTCCC ACTGCACATA TCCGTCCTTC TCATCCCCTA
    AAAAGCTTTG CTGTTCCAGC TGTGCCCACC TCTGCTTCTG GAGCTTCGTA TGAACCCTCA
    TTACCTAGCT CTCCGCTTCT TCAACAACAA TGCCCCACCG CTCTGTCTGT AAAGACAGCC
    TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC CCTCTTCCAG TCACAGTTCC CAGTGCCCCT
    GAACCACTAG AGACAAGCAG GATGCTAGAG GGCTCAGAGT GTAGCTATGA ACCGGACGAG
    TTAAGCAAAG AGATGGCTCA CCTTGAAGGC CTGATGAAGG ATCTAAATGC CATTACCACA
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32301
    Clone ID Related Accession (Same CDS sequence) XM_012966145.2 , NM_001374564.1
    Accession Version XM_012966145.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA
    GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA
    AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT
    TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC
    ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG
    AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC
    CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG
    ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA
    AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC
    TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT
    CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC
    TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG
    CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT
    GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA
    CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA
    GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT
    CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC
    AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC
    AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT
    CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821599.1
    CDS236..4543
    Translation

    Target ORF information:

    RefSeq Version XM_012966145.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966145.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA
    GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA
    AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT
    TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC
    ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG
    AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC
    CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG
    ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA
    AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC
    TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT
    CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC
    TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG
    CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT
    GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA
    CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA
    GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT
    CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC
    AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC
    AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT
    CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32302
    Clone ID Related Accession (Same CDS sequence) XM_012966146.2
    Accession Version XM_012966146.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966146.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT
    GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG
    TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT
    AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC
    CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC
    ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC
    TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA
    TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC
    CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG
    GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC
    CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821600.1
    CDS236..4531
    Translation

    Target ORF information:

    RefSeq Version XM_012966146.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966146.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT
    GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG
    TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT
    AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC
    CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC
    ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC
    TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA
    TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC
    CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG
    GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC
    CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32303
    Clone ID Related Accession (Same CDS sequence) XM_012966147.2
    Accession Version XM_012966147.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966147.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC
    CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG
    AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC
    ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC
    AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC
    AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA
    GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA
    GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT
    CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT
    AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC
    CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG
    GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA
    GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC
    TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT
    TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG
    TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT
    GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT
    CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT
    GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT
    GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821601.1
    CDS236..4513
    Translation

    Target ORF information:

    RefSeq Version XM_012966147.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966147.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC
    CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG
    AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC
    ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC
    AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC
    AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA
    GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA
    GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT
    CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT
    AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC
    CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG
    GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA
    GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC
    TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT
    TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG
    TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT
    GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT
    CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT
    GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT
    GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32304
    Clone ID Related Accession (Same CDS sequence) XM_012966148.2
    Accession Version XM_012966148.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT
    GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC
    CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA
    GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT
    CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG
    CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT
    GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA
    AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG
    GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821602.1
    CDS237..4487
    Translation

    Target ORF information:

    RefSeq Version XM_012966148.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966148.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT
    GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC
    CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA
    GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT
    CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG
    CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT
    GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA
    AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG
    GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32305
    Clone ID Related Accession (Same CDS sequence) XM_012966149.2
    Accession Version XM_012966149.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966149.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA
    GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA
    AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT
    TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC
    ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG
    AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC
    CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG
    ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA
    AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC
    TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT
    CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC
    TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG
    CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT
    GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA
    CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA
    GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT
    CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC
    AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC
    AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT
    CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821603.1
    CDS236..4483
    Translation

    Target ORF information:

    RefSeq Version XM_012966149.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966149.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA
    GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA
    AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT
    TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC
    ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG
    AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC
    CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG
    ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA
    AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC
    TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT
    CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC
    TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG
    CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT
    GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA
    CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA
    GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT
    CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC
    AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC
    AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT
    CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32306
    Clone ID Related Accession (Same CDS sequence) XM_012966150.2
    Accession Version XM_012966150.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC
    CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG
    AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC
    ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC
    AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC
    AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA
    GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA
    GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT
    CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT
    AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC
    CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG
    GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA
    GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC
    TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT
    TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG
    TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT
    GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT
    CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT
    GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT
    GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821604.1
    CDS236..4453
    Translation

    Target ORF information:

    RefSeq Version XM_012966150.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966150.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC
    CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG
    AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC
    ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC
    AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC
    AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA
    GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA
    GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT
    CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT
    AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC
    CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG
    GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA
    GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC
    TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT
    TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG
    TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT
    GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT
    CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT
    GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT
    GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32307
    Clone ID Related Accession (Same CDS sequence) XM_012966151.2
    Accession Version XM_012966151.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966151.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT
    GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC
    CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA
    GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT
    CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG
    CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT
    GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA
    AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG
    GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821605.1
    CDS237..4427
    Translation

    Target ORF information:

    RefSeq Version XM_012966151.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966151.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT
    GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC
    CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA
    GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT
    CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG
    CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT
    GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA
    AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG
    GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43037
    Clone ID Related Accession (Same CDS sequence) XM_018091948.1
    Accession Version XM_018091948.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGTCTCCCT
    CGATTTACTA GCCAGCCAGA GTCCTCTTCT GTATATGTTG GGGATAGCAT TGTATTGAAC
    TGTGATGTCA GTCCTGAACT GGTGCCTTTT GTGCATTGGG AGCAAAACAA GGCTTCACGA
    GAATTGGACA ACAGAGTAGC TGTCCTGTTA AATGGCAGCT TGGTCATTAG CAATGCCAAT
    GAAAGTGATG CTGGGCTATA CCGCTGTGCA GTAGGTGGTG GGTCATCCAT TAAATACAGT
    GAGGAGGCAG AGATTAGAGT ACTTCAAGAC ACAGGAGACG AGCGCCCCAT GCTGTTTCTT
    CGCCAGCCTT TGCCACTTAC CAGAGTTTCT GGGCAGACTG CACTCTTGAC TTGTGTGGTA
    TCTGGGTATC CAACACCCAG TGTGCGCTGG ACTCATAACC AGAAAGAAAT ACTGACTGAA
    AACTCAGAGA CGTTAACATT CGTGGCAGGG GGCAGCCTGC AGATCAGTTC TGTAACGGAA
    GAAGATTCTG GAATTTACAC ATGCATTGCC GACAATGGGA ACCAGACTAT TCAGGCTCAT
    GCTGAGCTTA GCATACAAGT ACCGCCTATT TTCCATGTCA AGCCATCCAA TACTCATGCA
    CACGAGTCCA TGGACATTGT ATTTAAGTGT GAGGTAACCG GGAAACCGAC ACCCACAGTT
    AAATGGGTTA AAAATGGAGA TATGGTGATT CCAAGTGACT ACTTCAAAAT AGTGGAGGAC
    CATGACCTGC AGGTGCTTGG TCTGGTGCGC TCAGATGAGG GATTCTATCA GTGTATAGCT
    GAAAATGAGG TGGGAAACGT CCAGGCAGCA GCACAGCTCA TTATCCTTGA GCCTGGTGTT
    ACCATCCCAA CCTCTCCCAC CCCATCACTG ACCCGTGCCA CTGCTCACAA TGTAGCTGCC
    ACTCCAGGAG GCCCCCTGCC TTCATCCCCA AGGGATGTCG TAGCCTCTCT TGTGTCTACA
    CGTTTCATAA AGCTGACTTG GCGTATGCCG GCTGATCCAC ATGGAGAGAA TCTCACCTAT
    CAGGTCTACT ATAGCAAGGA AAGCATGAAT AGGGAGCGAG TGGAGAACAC GAGCCGCCCA
    GGAGAAATGC AAGTTACCAT CCAGAACCTT CTACCCGAGA CAGTCTATTA CTTTCGAGTG
    GTGGCTCAAA ATCAGAATGG AGCAGGTGAA AGCTCTCTGC CTCTAAAGGT TGAAACACAG
    CCTGAAGTAC AAGTTCCTGG CCCAGCCCCC AACTTTAAAG CCACACCAGT CTCACCCACA
    TCTGTAAGTG TCTCTTGGGA AACCCCACTG TCTGGAAATG GGGAGATTCA GAGCTACAAG
    CTTTATTATA TGGAAAAAGG AGCAGACAAT GAACAGGATG TTGATGTGGG TGGACATTCA
    TATACATTAA ATGGCCTGAA AAAATATACA GAGTATACCT TCAGGGTGGT AGCCTTTAAC
    AAGCATGGGC CAGGTGTATC CACGCAGGAT GTGACTGTCA GGACTCTGTC AGATGTACCC
    AGTGCTGCCC CTCAGAACCT GACACTAGAA GTTCGGAGTT CACAGAGCAT TCTAGTGCAG
    TGGCAGCCCC CTCCTATAGG TTCCCAAAAT GGACAGATTG TGGGCTACAA GGTCCGTTAC
    CGCAAGACAA CAAGAAAGAG TGAGACTAGT GAGATTCTTG TGGATGCTCA GCTATCATAT
    CTTTTCACAG CTCTTGAGCG GGACACCGAG TACAGTTTTC GGGCTGTGGC CTTGACTGTT
    AATGGAAGTG GCCCAGCCTC AGACTGGGTT TCAGCAGAAA CCTTTGAGAG TGATCTGGAT
    GAGTCGCGTG TACCAGATGT GCCAAGCTCC CTCCATGTAC GTCCTTTGGT CACAAACATT
    GTAGTGAGCT GGACCCCCCC AGAAAACCAG CACCAGGTGG TGGTTCGTGG ATACGCAATT
    GGCTATGGCT TAGGAAGTCC ACATGCCCAG ACTGTACGTG TGGACCACAA GCAGCGTTAC
    TATACCATTG ATAACCTTGA ACCCAGCTCC CACTATGTTA TCTCGCTTAG AGCTTTTAAC
    AATGTTGGAG AGGGAATTCC ACTGTATGAG AGTGCAGTCA CCCGGCAGCA CACAGACCCT
    TCCGATGTTG ATGTGTTTGT TGTTCATGCC CCATACACCC CAGTTCCAGA GCCCAGTCCT
    ATGTTGCCCC CAGTTGGCGT GCAAGCCACT ATCGTGGCCA GTGACACTGT GCGTCTCACA
    TGGGCGGATA ACTCTCTCCC CAAGAACCAG AAGATCACTG ATTCAAGATA TTACACAGTG
    AGATGGAAGA CCAATATTCC AGCAAATACC AAATACAAGA TGGCAAACAG CACAACCTTA
    AGTTACTTGG TTACTGGTCT GAAGCCCAAC ACTTTATATG AGTTCTCAGT CATGGTGACC
    AAAGGTCGTA GATCAAGTAC ATGGAGTATG ACAGCACATG GGACTACATT TGAATCAGTG
    CCTTCCTCAC CTCCCAAAGA TGTAACTGTT GTGAGCAAGG AAGGAAAACC TCGCACCATT
    ATTGTCAACT GGCAGCCTCC CTCAGAAGCC AATGGTAAAA TAACAGGTTA TATTATATAT
    TACAGTACAG ATGCTCAGGC TGAACTCCAT GACTGGGTGA TTGAGCCTGT GATTGGAAAC
    CGTCTCACCC ACCAGATACA AGAGTTGACT CTAGACACCC CTTATTATTT TAAGATCCAG
    GCAAGAAATT CTAAGGGCAT GGGTCCTATG TCAGAAGCTG TGCTTTTCCG AACACCAAAG
    GAGTCATCAC CTTACATGCC TAACGACCAA GCATCCAGCA TCTCTGGGAA AGGAGTTCGC
    CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG
    AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC
    ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC
    AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC
    AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA
    GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA
    GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT
    CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT
    AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC
    CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG
    GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA
    GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC
    TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT
    TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG
    TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT
    GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT
    CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT
    GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT
    GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947437.1
    CDS236..4273
    Translation

    Target ORF information:

    RefSeq Version XM_018091948.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091948.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGTCTCCCT
    CGATTTACTA GCCAGCCAGA GTCCTCTTCT GTATATGTTG GGGATAGCAT TGTATTGAAC
    TGTGATGTCA GTCCTGAACT GGTGCCTTTT GTGCATTGGG AGCAAAACAA GGCTTCACGA
    GAATTGGACA ACAGAGTAGC TGTCCTGTTA AATGGCAGCT TGGTCATTAG CAATGCCAAT
    GAAAGTGATG CTGGGCTATA CCGCTGTGCA GTAGGTGGTG GGTCATCCAT TAAATACAGT
    GAGGAGGCAG AGATTAGAGT ACTTCAAGAC ACAGGAGACG AGCGCCCCAT GCTGTTTCTT
    CGCCAGCCTT TGCCACTTAC CAGAGTTTCT GGGCAGACTG CACTCTTGAC TTGTGTGGTA
    TCTGGGTATC CAACACCCAG TGTGCGCTGG ACTCATAACC AGAAAGAAAT ACTGACTGAA
    AACTCAGAGA CGTTAACATT CGTGGCAGGG GGCAGCCTGC AGATCAGTTC TGTAACGGAA
    GAAGATTCTG GAATTTACAC ATGCATTGCC GACAATGGGA ACCAGACTAT TCAGGCTCAT
    GCTGAGCTTA GCATACAAGT ACCGCCTATT TTCCATGTCA AGCCATCCAA TACTCATGCA
    CACGAGTCCA TGGACATTGT ATTTAAGTGT GAGGTAACCG GGAAACCGAC ACCCACAGTT
    AAATGGGTTA AAAATGGAGA TATGGTGATT CCAAGTGACT ACTTCAAAAT AGTGGAGGAC
    CATGACCTGC AGGTGCTTGG TCTGGTGCGC TCAGATGAGG GATTCTATCA GTGTATAGCT
    GAAAATGAGG TGGGAAACGT CCAGGCAGCA GCACAGCTCA TTATCCTTGA GCCTGGTGTT
    ACCATCCCAA CCTCTCCCAC CCCATCACTG ACCCGTGCCA CTGCTCACAA TGTAGCTGCC
    ACTCCAGGAG GCCCCCTGCC TTCATCCCCA AGGGATGTCG TAGCCTCTCT TGTGTCTACA
    CGTTTCATAA AGCTGACTTG GCGTATGCCG GCTGATCCAC ATGGAGAGAA TCTCACCTAT
    CAGGTCTACT ATAGCAAGGA AAGCATGAAT AGGGAGCGAG TGGAGAACAC GAGCCGCCCA
    GGAGAAATGC AAGTTACCAT CCAGAACCTT CTACCCGAGA CAGTCTATTA CTTTCGAGTG
    GTGGCTCAAA ATCAGAATGG AGCAGGTGAA AGCTCTCTGC CTCTAAAGGT TGAAACACAG
    CCTGAAGTAC AAGTTCCTGG CCCAGCCCCC AACTTTAAAG CCACACCAGT CTCACCCACA
    TCTGTAAGTG TCTCTTGGGA AACCCCACTG TCTGGAAATG GGGAGATTCA GAGCTACAAG
    CTTTATTATA TGGAAAAAGG AGCAGACAAT GAACAGGATG TTGATGTGGG TGGACATTCA
    TATACATTAA ATGGCCTGAA AAAATATACA GAGTATACCT TCAGGGTGGT AGCCTTTAAC
    AAGCATGGGC CAGGTGTATC CACGCAGGAT GTGACTGTCA GGACTCTGTC AGATGTACCC
    AGTGCTGCCC CTCAGAACCT GACACTAGAA GTTCGGAGTT CACAGAGCAT TCTAGTGCAG
    TGGCAGCCCC CTCCTATAGG TTCCCAAAAT GGACAGATTG TGGGCTACAA GGTCCGTTAC
    CGCAAGACAA CAAGAAAGAG TGAGACTAGT GAGATTCTTG TGGATGCTCA GCTATCATAT
    CTTTTCACAG CTCTTGAGCG GGACACCGAG TACAGTTTTC GGGCTGTGGC CTTGACTGTT
    AATGGAAGTG GCCCAGCCTC AGACTGGGTT TCAGCAGAAA CCTTTGAGAG TGATCTGGAT
    GAGTCGCGTG TACCAGATGT GCCAAGCTCC CTCCATGTAC GTCCTTTGGT CACAAACATT
    GTAGTGAGCT GGACCCCCCC AGAAAACCAG CACCAGGTGG TGGTTCGTGG ATACGCAATT
    GGCTATGGCT TAGGAAGTCC ACATGCCCAG ACTGTACGTG TGGACCACAA GCAGCGTTAC
    TATACCATTG ATAACCTTGA ACCCAGCTCC CACTATGTTA TCTCGCTTAG AGCTTTTAAC
    AATGTTGGAG AGGGAATTCC ACTGTATGAG AGTGCAGTCA CCCGGCAGCA CACAGACCCT
    TCCGATGTTG ATGTGTTTGT TGTTCATGCC CCATACACCC CAGTTCCAGA GCCCAGTCCT
    ATGTTGCCCC CAGTTGGCGT GCAAGCCACT ATCGTGGCCA GTGACACTGT GCGTCTCACA
    TGGGCGGATA ACTCTCTCCC CAAGAACCAG AAGATCACTG ATTCAAGATA TTACACAGTG
    AGATGGAAGA CCAATATTCC AGCAAATACC AAATACAAGA TGGCAAACAG CACAACCTTA
    AGTTACTTGG TTACTGGTCT GAAGCCCAAC ACTTTATATG AGTTCTCAGT CATGGTGACC
    AAAGGTCGTA GATCAAGTAC ATGGAGTATG ACAGCACATG GGACTACATT TGAATCAGTG
    CCTTCCTCAC CTCCCAAAGA TGTAACTGTT GTGAGCAAGG AAGGAAAACC TCGCACCATT
    ATTGTCAACT GGCAGCCTCC CTCAGAAGCC AATGGTAAAA TAACAGGTTA TATTATATAT
    TACAGTACAG ATGCTCAGGC TGAACTCCAT GACTGGGTGA TTGAGCCTGT GATTGGAAAC
    CGTCTCACCC ACCAGATACA AGAGTTGACT CTAGACACCC CTTATTATTT TAAGATCCAG
    GCAAGAAATT CTAAGGGCAT GGGTCCTATG TCAGAAGCTG TGCTTTTCCG AACACCAAAG
    GAGTCATCAC CTTACATGCC TAACGACCAA GCATCCAGCA TCTCTGGGAA AGGAGTTCGC
    CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG
    AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC
    ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC
    AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC
    AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA
    GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA
    GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT
    CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT
    AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC
    CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG
    GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA
    GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC
    TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT
    TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG
    TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT
    GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT
    CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT
    GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT
    GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53315
    Clone ID Related Accession (Same CDS sequence) XM_012966143.3
    Accession Version XM_012966143.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966143.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT
    GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG
    TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT
    AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC
    CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC
    ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC
    TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA
    TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC
    CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG
    GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC
    CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821597.1
    CDS235..4590
    Translation

    Target ORF information:

    RefSeq Version XM_012966143.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966143.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT
    GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG
    TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT
    AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC
    CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC
    ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC
    TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA
    TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC
    CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG
    GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC
    CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53316
    Clone ID Related Accession (Same CDS sequence) XM_012966144.3
    Accession Version XM_012966144.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966144.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGC ATCCAGCATC TCTGGGAAAG GAGTTCGCCC TTCTGATCCT
    GGATCAAATG GTGGCTCAAG TCTTGGAGGC AGTAATAGTC ACCATGGGAA CCCAACGTCA
    CTGGATCAGA ACATGCTTTT AACAATTATT GTTTCCATTG GTGTGATCAC TGTCTTGATT
    GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC CGTTCTAGCT CCCACCACAA AAAAAAGAGA
    GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT AAATACAAAG GCAACTCCAA GGATGTGAAA
    CCACCAGACC TGTGGATCCA TCATGAACGT TTGGAATTGA AACCAACAGA CAAGTCCCCA
    GAAACAAATC CTATTTTGAC AGACACGCCT CTGCCACGCT CCCCACAAGA TCTCACACCA
    GGGGATAGCA CCACGGAAAG CAGCATCCAC CAGCACAGCA ATTCGTATCG AGGTCTGGAA
    TCAGAAGATT CTGTGTCCTC ACTTGCTGGG CGCCGTGGCA TGCGACCTAA GATAATGATG
    CCTTTTGACT CCCAGCCTCC ACAACCGGTT GTCAGTGCCC ATCCCATCCA TTCCCACGAT
    AATTCTCATC ATTTTCGCTC CGTTCCTGGG TTCATGCTGC ACGTGTTGGG TGGCCCTCCA
    CAGGCTGCAG AATCTGTGCG TCATACCCCT AGCTGTGACA CTGTAGTAGC TGCATCATCT
    TCCTCTCAGA CTGGCCCTGA AGATCCAGAC CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC
    CTGGATGAAG ATTCAGTACC TAGTGTTCCC ACTGCACATA TCCGTCCTTC TCATCCCCTA
    AAAAGCTTTG CTGTTCCAGC TGTGCCCACC TCTGCTTCTG GAGCTTCGTA TGAACCCTCA
    TTACCTAGCT CTCCGCTTCT TCAACAACAA TGCCCCACCG CTCTGTCTGT AAAGACAGCC
    TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC CCTCTTCCAG TCACAGTTCC CAGTGCCCCT
    GAACCACTAG AGACAAGCAG GATGCTAGAG GGCTCAGAGT GTAGCTATGA ACCGGACGAG
    TTAAGCAAAG AGATGGCTCA CCTTGAAGGC CTGATGAAGG ATCTAAATGC CATTACCACA
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821598.1
    CDS235..4560
    Translation

    Target ORF information:

    RefSeq Version XM_012966144.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966144.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGC ATCCAGCATC TCTGGGAAAG GAGTTCGCCC TTCTGATCCT
    GGATCAAATG GTGGCTCAAG TCTTGGAGGC AGTAATAGTC ACCATGGGAA CCCAACGTCA
    CTGGATCAGA ACATGCTTTT AACAATTATT GTTTCCATTG GTGTGATCAC TGTCTTGATT
    GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC CGTTCTAGCT CCCACCACAA AAAAAAGAGA
    GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT AAATACAAAG GCAACTCCAA GGATGTGAAA
    CCACCAGACC TGTGGATCCA TCATGAACGT TTGGAATTGA AACCAACAGA CAAGTCCCCA
    GAAACAAATC CTATTTTGAC AGACACGCCT CTGCCACGCT CCCCACAAGA TCTCACACCA
    GGGGATAGCA CCACGGAAAG CAGCATCCAC CAGCACAGCA ATTCGTATCG AGGTCTGGAA
    TCAGAAGATT CTGTGTCCTC ACTTGCTGGG CGCCGTGGCA TGCGACCTAA GATAATGATG
    CCTTTTGACT CCCAGCCTCC ACAACCGGTT GTCAGTGCCC ATCCCATCCA TTCCCACGAT
    AATTCTCATC ATTTTCGCTC CGTTCCTGGG TTCATGCTGC ACGTGTTGGG TGGCCCTCCA
    CAGGCTGCAG AATCTGTGCG TCATACCCCT AGCTGTGACA CTGTAGTAGC TGCATCATCT
    TCCTCTCAGA CTGGCCCTGA AGATCCAGAC CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC
    CTGGATGAAG ATTCAGTACC TAGTGTTCCC ACTGCACATA TCCGTCCTTC TCATCCCCTA
    AAAAGCTTTG CTGTTCCAGC TGTGCCCACC TCTGCTTCTG GAGCTTCGTA TGAACCCTCA
    TTACCTAGCT CTCCGCTTCT TCAACAACAA TGCCCCACCG CTCTGTCTGT AAAGACAGCC
    TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC CCTCTTCCAG TCACAGTTCC CAGTGCCCCT
    GAACCACTAG AGACAAGCAG GATGCTAGAG GGCTCAGAGT GTAGCTATGA ACCGGACGAG
    TTAAGCAAAG AGATGGCTCA CCTTGAAGGC CTGATGAAGG ATCTAAATGC CATTACCACA
    GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53317
    Clone ID Related Accession (Same CDS sequence) XM_012966146.3
    Accession Version XM_012966146.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966146.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT
    GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG
    TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT
    AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC
    CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC
    ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC
    TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA
    TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC
    CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG
    GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC
    CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821600.1
    CDS235..4530
    Translation

    Target ORF information:

    RefSeq Version XM_012966146.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966146.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT
    GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG
    TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT
    AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC
    CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC
    ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC
    TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA
    TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC
    CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG
    GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC
    CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53318
    Clone ID Related Accession (Same CDS sequence) XM_012966147.3
    Accession Version XM_012966147.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966147.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACCACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC
    CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG
    AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC
    ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC
    AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC
    AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA
    GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA
    GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT
    CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT
    AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC
    CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG
    GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA
    GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC
    TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT
    TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG
    TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT
    GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT
    CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT
    GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT
    GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821601.1
    CDS235..4512
    Translation

    Target ORF information:

    RefSeq Version XM_012966147.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966147.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACCACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC
    CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG
    AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC
    ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC
    AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC
    AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA
    GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA
    GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT
    CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT
    AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC
    CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG
    GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA
    GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC
    TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT
    TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG
    TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT
    GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT
    CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT
    GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT
    GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53319
    Clone ID Related Accession (Same CDS sequence) XM_012966148.3
    Accession Version XM_012966148.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT
    GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC
    CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA
    GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT
    CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG
    CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT
    GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA
    AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG
    GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821602.1
    CDS236..4486
    Translation

    Target ORF information:

    RefSeq Version XM_012966148.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966148.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT
    GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT
    GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC
    CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA
    GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT
    CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG
    CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT
    GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA
    AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG
    GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53320
    Clone ID Related Accession (Same CDS sequence) XM_012966149.3
    Accession Version XM_012966149.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966149.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACCACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA
    GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA
    AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT
    TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC
    ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG
    AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC
    CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG
    ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA
    AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC
    TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT
    CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC
    TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG
    CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT
    GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA
    CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA
    GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT
    CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC
    AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC
    AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT
    CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821603.1
    CDS235..4482
    Translation

    Target ORF information:

    RefSeq Version XM_012966149.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966149.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACCACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA
    GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA
    AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT
    TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC
    ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG
    AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC
    CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG
    ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA
    AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC
    TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT
    CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC
    TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG
    CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT
    GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA
    CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA
    GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT
    CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC
    AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC
    AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT
    CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53321
    Clone ID Related Accession (Same CDS sequence) XM_012966150.3
    Accession Version XM_012966150.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966150.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACCACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC
    CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG
    AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC
    ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC
    AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC
    AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA
    GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA
    GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT
    CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT
    AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC
    CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG
    GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA
    GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC
    TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT
    TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG
    TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT
    GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT
    CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT
    GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT
    GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821604.1
    CDS235..4452
    Translation

    Target ORF information:

    RefSeq Version XM_012966150.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966150.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT
    ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG
    AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC
    AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC
    ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG
    ACAGCACATG GGACCACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT
    GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC
    AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT
    GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT
    CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG
    TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC
    CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG
    AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC
    ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC
    AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC
    AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA
    GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA
    GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT
    CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT
    AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC
    CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG
    GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA
    GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC
    TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT
    TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG
    TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT
    GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT
    CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT
    GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT
    GCCATTACCA CAGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53322
    Clone ID Related Accession (Same CDS sequence) XM_012966151.3
    Accession Version XM_012966151.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product neogenin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966151.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT
    GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC
    CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA
    GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT
    CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG
    CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT
    GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA
    AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG
    GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821605.1
    CDS236..4426
    Translation

    Target ORF information:

    RefSeq Version XM_012966151.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neogenin 1 (neo1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966151.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
    TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA
    TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG
    GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG
    AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT
    GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC
    TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC
    TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG
    GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG
    CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG
    GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG
    TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG
    CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA
    CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG
    AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG
    ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC
    CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG
    CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG
    AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC
    TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA
    TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT
    ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA
    GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT
    GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG
    GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA
    GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT
    CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC
    ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG
    GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT
    GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC
    AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG
    ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA
    CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG
    GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG
    GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG
    GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC
    TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT
    CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG
    GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG
    GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC
    TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC
    CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA
    GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT
    GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT
    TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG
    GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG
    TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG
    ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA
    GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA
    ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT
    GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT
    TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG
    CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC
    TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC
    AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT
    GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC
    CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT
    AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT
    TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT
    CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC
    CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG
    CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT
    GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC
    CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA
    GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT
    CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG
    CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT
    GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA
    AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG
    GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.