neo1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012966144.2	XP_012821598.1	neogenin isoform X2
XM_012966150.2	XP_012821604.1	neogenin isoform X8
XM_012966143.3	XP_012821597.1	neogenin isoform X1
XM_012966149.2	XP_012821603.1	neogenin isoform X7
XM_012966147.3	XP_012821601.1	neogenin isoform X4
XM_012966145.2	XP_012821599.1	neogenin isoform X3
XM_012966146.2	XP_012821600.1	neogenin isoform X4
XM_012966148.2	XP_012821602.1	neogenin isoform X6
XM_018091948.1	XP_017947437.1	neogenin isoform X9
NM_001129940.1	NP_001123412.1	neogenin
NM_001374564.1	NP_001361493.1	neogenin
XM_012966143.2	XP_012821597.1	neogenin isoform X1
XM_012966144.3	XP_012821598.1	neogenin isoform X2
XM_012966146.3	XP_012821600.1	neogenin isoform X3
XM_012966150.3	XP_012821604.1	neogenin isoform X7
XM_012966147.2	XP_012821601.1	neogenin isoform X5
XM_012966151.2	XP_012821605.1	neogenin isoform X10
XM_012966148.3	XP_012821602.1	neogenin isoform X5
XM_012966149.3	XP_012821603.1	neogenin isoform X6
XM_012966151.3	XP_012821605.1	neogenin isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa24188	NM_001129940.1 Latest version!	Xenopus tropicalis neogenin 1 (neo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa32301	NM_001374564.1 Latest version!	Xenopus tropicalis neogenin 1 (neo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32299	XM_012966143.2 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32300	XM_012966144.2 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32301	XM_012966145.2 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32302	XM_012966146.2 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32303	XM_012966147.2 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32304	XM_012966148.2 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32305	XM_012966149.2 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32306	XM_012966150.2 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32307	XM_012966151.2 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa43037	XM_018091948.1 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53315	XM_012966143.3 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53316	XM_012966144.3 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53317	XM_012966146.3 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53318	XM_012966147.3 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53319	XM_012966148.3 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53320	XM_012966149.3 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53321	XM_012966150.3 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53322	XM_012966151.3 Latest version!	Xenopus tropicalis neogenin 1 (neo1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

neo1 ( NM_001129940.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001123412.1 Xenopus tropicalis neogenin 1 (neo1), mRNA.

Price & Availability↑

CloneID	OXa24188
Clone ID Related Accession (Same CDS sequence)	NM_001129940.1
Accession Version	NM_001129940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-28
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167600.1. ##Evidence-Data-START## Transcript exon combination :: BC167600.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1025683, SAMEA1025684 [ECO:0000348] ##Evidence-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGTGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGGTAAGTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123412.1
CDS	130..2673
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPGVTIPTSPTPSLTRATAHNVAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTGKS

Target ORF information:

RefSeq Version	NM_001129940.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129940.1

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGTGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGGTAAGTC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( NM_001374564.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001361493.1 Xenopus tropicalis neogenin 1 (neo1), mRNA.

Price & Availability↑

CloneID	OXa32301
Clone ID Related Accession (Same CDS sequence)	XM_012966145.2 , NM_001374564.1
Accession Version	NM_001374564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-03
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03012089.1 and AAMC03012090.1. On Oct 4, 2019 this sequence version replaced XM_012966145.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1025683, SAMEA1025684 [ECO:0000348] ##Evidence-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA 
GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA 
AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT 
TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC 
ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG 
AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC 
CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG 
ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA 
AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC 
TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT 
CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC 
TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG 
CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT 
GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA 
CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA 
GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT 
CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC 
AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC 
AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT 
CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361493.1
CDS	130..4437
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPGVTIPTSPTPSLTRATAHNVAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	NM_001374564.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374564.1

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA 
GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA 
AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT 
TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC 
ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG 
AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC 
CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG 
ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA 
AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC 
TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT 
CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC 
TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG 
CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT 
GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA 
CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA 
GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT 
CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC 
AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC 
AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT 
CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966143.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821597.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X1, mRNA.

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CloneID	OXa32299
Clone ID Related Accession (Same CDS sequence)	XM_012966143.2
Accession Version	XM_012966143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT 
GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG 
TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT 
AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC 
CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC 
ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC 
TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA 
TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC 
CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG 
GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC 
CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821597.1
CDS	236..4591
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPGVTIPTSPTPSLTRATAHNVAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTDPSDVDVFVVHAPYTPVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966143.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966143.2

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT 
GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG 
TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT 
AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC 
CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC 
ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC 
TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA 
TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC 
CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG 
GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC 
CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966144.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821598.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa32300
Clone ID Related Accession (Same CDS sequence)	XM_012966144.2
Accession Version	XM_012966144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGC ATCCAGCATC TCTGGGAAAG GAGTTCGCCC TTCTGATCCT 
GGATCAAATG GTGGCTCAAG TCTTGGAGGC AGTAATAGTC ACCATGGGAA CCCAACGTCA 
CTGGATCAGA ACATGCTTTT AACAATTATT GTTTCCATTG GTGTGATCAC TGTCTTGATT 
GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC CGTTCTAGCT CCCACCACAA AAAAAAGAGA 
GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT AAATACAAAG GCAACTCCAA GGATGTGAAA 
CCACCAGACC TGTGGATCCA TCATGAACGT TTGGAATTGA AACCAACAGA CAAGTCCCCA 
GAAACAAATC CTATTTTGAC AGACACGCCT CTGCCACGCT CCCCACAAGA TCTCACACCA 
GGGGATAGCA CCACGGAAAG CAGCATCCAC CAGCACAGCA ATTCGTATCG AGGTCTGGAA 
TCAGAAGATT CTGTGTCCTC ACTTGCTGGG CGCCGTGGCA TGCGACCTAA GATAATGATG 
CCTTTTGACT CCCAGCCTCC ACAACCGGTT GTCAGTGCCC ATCCCATCCA TTCCCACGAT 
AATTCTCATC ATTTTCGCTC CGTTCCTGGG TTCATGCTGC ACGTGTTGGG TGGCCCTCCA 
CAGGCTGCAG AATCTGTGCG TCATACCCCT AGCTGTGACA CTGTAGTAGC TGCATCATCT 
TCCTCTCAGA CTGGCCCTGA AGATCCAGAC CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC 
CTGGATGAAG ATTCAGTACC TAGTGTTCCC ACTGCACATA TCCGTCCTTC TCATCCCCTA 
AAAAGCTTTG CTGTTCCAGC TGTGCCCACC TCTGCTTCTG GAGCTTCGTA TGAACCCTCA 
TTACCTAGCT CTCCGCTTCT TCAACAACAA TGCCCCACCG CTCTGTCTGT AAAGACAGCC 
TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC CCTCTTCCAG TCACAGTTCC CAGTGCCCCT 
GAACCACTAG AGACAAGCAG GATGCTAGAG GGCTCAGAGT GTAGCTATGA ACCGGACGAG 
TTAAGCAAAG AGATGGCTCA CCTTGAAGGC CTGATGAAGG ATCTAAATGC CATTACCACA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821598.1
CDS	236..4561
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPGVTIPTSPTPSLTRATAHNVAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTDPSDVDVFVVHAPYTPVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966144.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966144.2

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGC ATCCAGCATC TCTGGGAAAG GAGTTCGCCC TTCTGATCCT 
GGATCAAATG GTGGCTCAAG TCTTGGAGGC AGTAATAGTC ACCATGGGAA CCCAACGTCA 
CTGGATCAGA ACATGCTTTT AACAATTATT GTTTCCATTG GTGTGATCAC TGTCTTGATT 
GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC CGTTCTAGCT CCCACCACAA AAAAAAGAGA 
GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT AAATACAAAG GCAACTCCAA GGATGTGAAA 
CCACCAGACC TGTGGATCCA TCATGAACGT TTGGAATTGA AACCAACAGA CAAGTCCCCA 
GAAACAAATC CTATTTTGAC AGACACGCCT CTGCCACGCT CCCCACAAGA TCTCACACCA 
GGGGATAGCA CCACGGAAAG CAGCATCCAC CAGCACAGCA ATTCGTATCG AGGTCTGGAA 
TCAGAAGATT CTGTGTCCTC ACTTGCTGGG CGCCGTGGCA TGCGACCTAA GATAATGATG 
CCTTTTGACT CCCAGCCTCC ACAACCGGTT GTCAGTGCCC ATCCCATCCA TTCCCACGAT 
AATTCTCATC ATTTTCGCTC CGTTCCTGGG TTCATGCTGC ACGTGTTGGG TGGCCCTCCA 
CAGGCTGCAG AATCTGTGCG TCATACCCCT AGCTGTGACA CTGTAGTAGC TGCATCATCT 
TCCTCTCAGA CTGGCCCTGA AGATCCAGAC CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC 
CTGGATGAAG ATTCAGTACC TAGTGTTCCC ACTGCACATA TCCGTCCTTC TCATCCCCTA 
AAAAGCTTTG CTGTTCCAGC TGTGCCCACC TCTGCTTCTG GAGCTTCGTA TGAACCCTCA 
TTACCTAGCT CTCCGCTTCT TCAACAACAA TGCCCCACCG CTCTGTCTGT AAAGACAGCC 
TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC CCTCTTCCAG TCACAGTTCC CAGTGCCCCT 
GAACCACTAG AGACAAGCAG GATGCTAGAG GGCTCAGAGT GTAGCTATGA ACCGGACGAG 
TTAAGCAAAG AGATGGCTCA CCTTGAAGGC CTGATGAAGG ATCTAAATGC CATTACCACA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966145.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821599.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa32301
Clone ID Related Accession (Same CDS sequence)	XM_012966145.2 , NM_001374564.1
Accession Version	XM_012966145.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA 
GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA 
AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT 
TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC 
ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG 
AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC 
CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG 
ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA 
AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC 
TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT 
CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC 
TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG 
CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT 
GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA 
CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA 
GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT 
CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC 
AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC 
AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT 
CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821599.1
CDS	236..4543
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPGVTIPTSPTPSLTRATAHNVAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966145.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966145.2

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA 
GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA 
AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT 
TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC 
ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG 
AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC 
CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG 
ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA 
AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC 
TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT 
CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC 
TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG 
CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT 
GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA 
CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA 
GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT 
CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC 
AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC 
AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT 
CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966146.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821600.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X4, mRNA.

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CloneID	OXa32302
Clone ID Related Accession (Same CDS sequence)	XM_012966146.2
Accession Version	XM_012966146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966146.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT 
GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG 
TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT 
AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC 
CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC 
ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC 
TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA 
TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC 
CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG 
GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC 
CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821600.1
CDS	236..4531
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTDPSDVDVFVVHAPYTPVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966146.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966146.2

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT 
GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG 
TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT 
AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC 
CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC 
ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC 
TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA 
TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC 
CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG 
GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC 
CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966147.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821601.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X5, mRNA.

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CloneID	OXa32303
Clone ID Related Accession (Same CDS sequence)	XM_012966147.2
Accession Version	XM_012966147.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966147.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC 
CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG 
AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC 
ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC 
AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC 
AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA 
GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA 
GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT 
CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT 
AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC 
CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG 
GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA 
GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC 
TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT 
TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG 
TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT 
GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT 
CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT 
GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT 
GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821601.1
CDS	236..4513
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPGVTIPTSPTPSLTRATAHNVAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966147.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966147.2

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC 
CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG 
AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC 
ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC 
AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC 
AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA 
GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA 
GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT 
CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT 
AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC 
CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG 
GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA 
GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC 
TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT 
TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG 
TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT 
GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT 
CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT 
GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT 
GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966148.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821602.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa32304
Clone ID Related Accession (Same CDS sequence)	XM_012966148.2
Accession Version	XM_012966148.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT 
GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC 
CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA 
GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT 
CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG 
CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT 
GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA 
AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG 
GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821602.1
CDS	237..4487
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPGVTIPTSPTPSLTRATAHNVAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTDPSDVDVFVVHAPYTPVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQQSVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966148.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966148.2

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT 
GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC 
CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA 
GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT 
CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG 
CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT 
GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA 
AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG 
GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966149.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821603.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa32305
Clone ID Related Accession (Same CDS sequence)	XM_012966149.2
Accession Version	XM_012966149.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966149.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA 
GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA 
AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT 
TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC 
ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG 
AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC 
CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG 
ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA 
AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC 
TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT 
CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC 
TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG 
CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT 
GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA 
CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA 
GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT 
CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC 
AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC 
AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT 
CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821603.1
CDS	236..4483
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966149.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966149.2

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA 
GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA 
AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT 
TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC 
ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG 
AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC 
CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG 
ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA 
AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC 
TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT 
CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC 
TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG 
CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT 
GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA 
CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA 
GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT 
CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC 
AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC 
AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT 
CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966150.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821604.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X8, mRNA.

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CloneID	OXa32306
Clone ID Related Accession (Same CDS sequence)	XM_012966150.2
Accession Version	XM_012966150.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC 
CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG 
AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC 
ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC 
AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC 
AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA 
GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA 
GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT 
CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT 
AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC 
CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG 
GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA 
GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC 
TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT 
TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG 
TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT 
GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT 
CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT 
GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT 
GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821604.1
CDS	236..4453
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966150.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966150.2

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACTACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC 
CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG 
AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC 
ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC 
AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC 
AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA 
GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA 
GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT 
CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT 
AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC 
CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG 
GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA 
GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC 
TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT 
TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG 
TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT 
GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT 
CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT 
GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT 
GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966151.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821605.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X10, mRNA.

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CloneID	OXa32307
Clone ID Related Accession (Same CDS sequence)	XM_012966151.2
Accession Version	XM_012966151.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966151.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT 
GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC 
CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA 
GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT 
CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG 
CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT 
GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA 
AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG 
GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821605.1
CDS	237..4427
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTDPSDVDVFVVHAPYTPVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQQSVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966151.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966151.2

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC TCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTTG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACTACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT 
GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC 
CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA 
GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT 
CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG 
CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT 
GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA 
AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG 
GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_018091948.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947437.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OXa43037
Clone ID Related Accession (Same CDS sequence)	XM_018091948.1
Accession Version	XM_018091948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGTCTCCCT 
CGATTTACTA GCCAGCCAGA GTCCTCTTCT GTATATGTTG GGGATAGCAT TGTATTGAAC 
TGTGATGTCA GTCCTGAACT GGTGCCTTTT GTGCATTGGG AGCAAAACAA GGCTTCACGA 
GAATTGGACA ACAGAGTAGC TGTCCTGTTA AATGGCAGCT TGGTCATTAG CAATGCCAAT 
GAAAGTGATG CTGGGCTATA CCGCTGTGCA GTAGGTGGTG GGTCATCCAT TAAATACAGT 
GAGGAGGCAG AGATTAGAGT ACTTCAAGAC ACAGGAGACG AGCGCCCCAT GCTGTTTCTT 
CGCCAGCCTT TGCCACTTAC CAGAGTTTCT GGGCAGACTG CACTCTTGAC TTGTGTGGTA 
TCTGGGTATC CAACACCCAG TGTGCGCTGG ACTCATAACC AGAAAGAAAT ACTGACTGAA 
AACTCAGAGA CGTTAACATT CGTGGCAGGG GGCAGCCTGC AGATCAGTTC TGTAACGGAA 
GAAGATTCTG GAATTTACAC ATGCATTGCC GACAATGGGA ACCAGACTAT TCAGGCTCAT 
GCTGAGCTTA GCATACAAGT ACCGCCTATT TTCCATGTCA AGCCATCCAA TACTCATGCA 
CACGAGTCCA TGGACATTGT ATTTAAGTGT GAGGTAACCG GGAAACCGAC ACCCACAGTT 
AAATGGGTTA AAAATGGAGA TATGGTGATT CCAAGTGACT ACTTCAAAAT AGTGGAGGAC 
CATGACCTGC AGGTGCTTGG TCTGGTGCGC TCAGATGAGG GATTCTATCA GTGTATAGCT 
GAAAATGAGG TGGGAAACGT CCAGGCAGCA GCACAGCTCA TTATCCTTGA GCCTGGTGTT 
ACCATCCCAA CCTCTCCCAC CCCATCACTG ACCCGTGCCA CTGCTCACAA TGTAGCTGCC 
ACTCCAGGAG GCCCCCTGCC TTCATCCCCA AGGGATGTCG TAGCCTCTCT TGTGTCTACA 
CGTTTCATAA AGCTGACTTG GCGTATGCCG GCTGATCCAC ATGGAGAGAA TCTCACCTAT 
CAGGTCTACT ATAGCAAGGA AAGCATGAAT AGGGAGCGAG TGGAGAACAC GAGCCGCCCA 
GGAGAAATGC AAGTTACCAT CCAGAACCTT CTACCCGAGA CAGTCTATTA CTTTCGAGTG 
GTGGCTCAAA ATCAGAATGG AGCAGGTGAA AGCTCTCTGC CTCTAAAGGT TGAAACACAG 
CCTGAAGTAC AAGTTCCTGG CCCAGCCCCC AACTTTAAAG CCACACCAGT CTCACCCACA 
TCTGTAAGTG TCTCTTGGGA AACCCCACTG TCTGGAAATG GGGAGATTCA GAGCTACAAG 
CTTTATTATA TGGAAAAAGG AGCAGACAAT GAACAGGATG TTGATGTGGG TGGACATTCA 
TATACATTAA ATGGCCTGAA AAAATATACA GAGTATACCT TCAGGGTGGT AGCCTTTAAC 
AAGCATGGGC CAGGTGTATC CACGCAGGAT GTGACTGTCA GGACTCTGTC AGATGTACCC 
AGTGCTGCCC CTCAGAACCT GACACTAGAA GTTCGGAGTT CACAGAGCAT TCTAGTGCAG 
TGGCAGCCCC CTCCTATAGG TTCCCAAAAT GGACAGATTG TGGGCTACAA GGTCCGTTAC 
CGCAAGACAA CAAGAAAGAG TGAGACTAGT GAGATTCTTG TGGATGCTCA GCTATCATAT 
CTTTTCACAG CTCTTGAGCG GGACACCGAG TACAGTTTTC GGGCTGTGGC CTTGACTGTT 
AATGGAAGTG GCCCAGCCTC AGACTGGGTT TCAGCAGAAA CCTTTGAGAG TGATCTGGAT 
GAGTCGCGTG TACCAGATGT GCCAAGCTCC CTCCATGTAC GTCCTTTGGT CACAAACATT 
GTAGTGAGCT GGACCCCCCC AGAAAACCAG CACCAGGTGG TGGTTCGTGG ATACGCAATT 
GGCTATGGCT TAGGAAGTCC ACATGCCCAG ACTGTACGTG TGGACCACAA GCAGCGTTAC 
TATACCATTG ATAACCTTGA ACCCAGCTCC CACTATGTTA TCTCGCTTAG AGCTTTTAAC 
AATGTTGGAG AGGGAATTCC ACTGTATGAG AGTGCAGTCA CCCGGCAGCA CACAGACCCT 
TCCGATGTTG ATGTGTTTGT TGTTCATGCC CCATACACCC CAGTTCCAGA GCCCAGTCCT 
ATGTTGCCCC CAGTTGGCGT GCAAGCCACT ATCGTGGCCA GTGACACTGT GCGTCTCACA 
TGGGCGGATA ACTCTCTCCC CAAGAACCAG AAGATCACTG ATTCAAGATA TTACACAGTG 
AGATGGAAGA CCAATATTCC AGCAAATACC AAATACAAGA TGGCAAACAG CACAACCTTA 
AGTTACTTGG TTACTGGTCT GAAGCCCAAC ACTTTATATG AGTTCTCAGT CATGGTGACC 
AAAGGTCGTA GATCAAGTAC ATGGAGTATG ACAGCACATG GGACTACATT TGAATCAGTG 
CCTTCCTCAC CTCCCAAAGA TGTAACTGTT GTGAGCAAGG AAGGAAAACC TCGCACCATT 
ATTGTCAACT GGCAGCCTCC CTCAGAAGCC AATGGTAAAA TAACAGGTTA TATTATATAT 
TACAGTACAG ATGCTCAGGC TGAACTCCAT GACTGGGTGA TTGAGCCTGT GATTGGAAAC 
CGTCTCACCC ACCAGATACA AGAGTTGACT CTAGACACCC CTTATTATTT TAAGATCCAG 
GCAAGAAATT CTAAGGGCAT GGGTCCTATG TCAGAAGCTG TGCTTTTCCG AACACCAAAG 
GAGTCATCAC CTTACATGCC TAACGACCAA GCATCCAGCA TCTCTGGGAA AGGAGTTCGC 
CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG 
AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC 
ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC 
AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC 
AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA 
GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA 
GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT 
CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT 
AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC 
CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG 
GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA 
GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC 
TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT 
TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG 
TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT 
GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT 
CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT 
GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT 
GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947437.1
CDS	236..4273
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPGVTIPTSPTPSLTRATAHNVAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTDPSDVDVFVVHAPYTPVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_018091948.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091948.1

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGTCTCCCT 
CGATTTACTA GCCAGCCAGA GTCCTCTTCT GTATATGTTG GGGATAGCAT TGTATTGAAC 
TGTGATGTCA GTCCTGAACT GGTGCCTTTT GTGCATTGGG AGCAAAACAA GGCTTCACGA 
GAATTGGACA ACAGAGTAGC TGTCCTGTTA AATGGCAGCT TGGTCATTAG CAATGCCAAT 
GAAAGTGATG CTGGGCTATA CCGCTGTGCA GTAGGTGGTG GGTCATCCAT TAAATACAGT 
GAGGAGGCAG AGATTAGAGT ACTTCAAGAC ACAGGAGACG AGCGCCCCAT GCTGTTTCTT 
CGCCAGCCTT TGCCACTTAC CAGAGTTTCT GGGCAGACTG CACTCTTGAC TTGTGTGGTA 
TCTGGGTATC CAACACCCAG TGTGCGCTGG ACTCATAACC AGAAAGAAAT ACTGACTGAA 
AACTCAGAGA CGTTAACATT CGTGGCAGGG GGCAGCCTGC AGATCAGTTC TGTAACGGAA 
GAAGATTCTG GAATTTACAC ATGCATTGCC GACAATGGGA ACCAGACTAT TCAGGCTCAT 
GCTGAGCTTA GCATACAAGT ACCGCCTATT TTCCATGTCA AGCCATCCAA TACTCATGCA 
CACGAGTCCA TGGACATTGT ATTTAAGTGT GAGGTAACCG GGAAACCGAC ACCCACAGTT 
AAATGGGTTA AAAATGGAGA TATGGTGATT CCAAGTGACT ACTTCAAAAT AGTGGAGGAC 
CATGACCTGC AGGTGCTTGG TCTGGTGCGC TCAGATGAGG GATTCTATCA GTGTATAGCT 
GAAAATGAGG TGGGAAACGT CCAGGCAGCA GCACAGCTCA TTATCCTTGA GCCTGGTGTT 
ACCATCCCAA CCTCTCCCAC CCCATCACTG ACCCGTGCCA CTGCTCACAA TGTAGCTGCC 
ACTCCAGGAG GCCCCCTGCC TTCATCCCCA AGGGATGTCG TAGCCTCTCT TGTGTCTACA 
CGTTTCATAA AGCTGACTTG GCGTATGCCG GCTGATCCAC ATGGAGAGAA TCTCACCTAT 
CAGGTCTACT ATAGCAAGGA AAGCATGAAT AGGGAGCGAG TGGAGAACAC GAGCCGCCCA 
GGAGAAATGC AAGTTACCAT CCAGAACCTT CTACCCGAGA CAGTCTATTA CTTTCGAGTG 
GTGGCTCAAA ATCAGAATGG AGCAGGTGAA AGCTCTCTGC CTCTAAAGGT TGAAACACAG 
CCTGAAGTAC AAGTTCCTGG CCCAGCCCCC AACTTTAAAG CCACACCAGT CTCACCCACA 
TCTGTAAGTG TCTCTTGGGA AACCCCACTG TCTGGAAATG GGGAGATTCA GAGCTACAAG 
CTTTATTATA TGGAAAAAGG AGCAGACAAT GAACAGGATG TTGATGTGGG TGGACATTCA 
TATACATTAA ATGGCCTGAA AAAATATACA GAGTATACCT TCAGGGTGGT AGCCTTTAAC 
AAGCATGGGC CAGGTGTATC CACGCAGGAT GTGACTGTCA GGACTCTGTC AGATGTACCC 
AGTGCTGCCC CTCAGAACCT GACACTAGAA GTTCGGAGTT CACAGAGCAT TCTAGTGCAG 
TGGCAGCCCC CTCCTATAGG TTCCCAAAAT GGACAGATTG TGGGCTACAA GGTCCGTTAC 
CGCAAGACAA CAAGAAAGAG TGAGACTAGT GAGATTCTTG TGGATGCTCA GCTATCATAT 
CTTTTCACAG CTCTTGAGCG GGACACCGAG TACAGTTTTC GGGCTGTGGC CTTGACTGTT 
AATGGAAGTG GCCCAGCCTC AGACTGGGTT TCAGCAGAAA CCTTTGAGAG TGATCTGGAT 
GAGTCGCGTG TACCAGATGT GCCAAGCTCC CTCCATGTAC GTCCTTTGGT CACAAACATT 
GTAGTGAGCT GGACCCCCCC AGAAAACCAG CACCAGGTGG TGGTTCGTGG ATACGCAATT 
GGCTATGGCT TAGGAAGTCC ACATGCCCAG ACTGTACGTG TGGACCACAA GCAGCGTTAC 
TATACCATTG ATAACCTTGA ACCCAGCTCC CACTATGTTA TCTCGCTTAG AGCTTTTAAC 
AATGTTGGAG AGGGAATTCC ACTGTATGAG AGTGCAGTCA CCCGGCAGCA CACAGACCCT 
TCCGATGTTG ATGTGTTTGT TGTTCATGCC CCATACACCC CAGTTCCAGA GCCCAGTCCT 
ATGTTGCCCC CAGTTGGCGT GCAAGCCACT ATCGTGGCCA GTGACACTGT GCGTCTCACA 
TGGGCGGATA ACTCTCTCCC CAAGAACCAG AAGATCACTG ATTCAAGATA TTACACAGTG 
AGATGGAAGA CCAATATTCC AGCAAATACC AAATACAAGA TGGCAAACAG CACAACCTTA 
AGTTACTTGG TTACTGGTCT GAAGCCCAAC ACTTTATATG AGTTCTCAGT CATGGTGACC 
AAAGGTCGTA GATCAAGTAC ATGGAGTATG ACAGCACATG GGACTACATT TGAATCAGTG 
CCTTCCTCAC CTCCCAAAGA TGTAACTGTT GTGAGCAAGG AAGGAAAACC TCGCACCATT 
ATTGTCAACT GGCAGCCTCC CTCAGAAGCC AATGGTAAAA TAACAGGTTA TATTATATAT 
TACAGTACAG ATGCTCAGGC TGAACTCCAT GACTGGGTGA TTGAGCCTGT GATTGGAAAC 
CGTCTCACCC ACCAGATACA AGAGTTGACT CTAGACACCC CTTATTATTT TAAGATCCAG 
GCAAGAAATT CTAAGGGCAT GGGTCCTATG TCAGAAGCTG TGCTTTTCCG AACACCAAAG 
GAGTCATCAC CTTACATGCC TAACGACCAA GCATCCAGCA TCTCTGGGAA AGGAGTTCGC 
CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG 
AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC 
ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC 
AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC 
AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA 
GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA 
GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT 
CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT 
AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC 
CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG 
GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA 
GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC 
TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT 
TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG 
TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT 
GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT 
CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT 
GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT 
GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966143.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821597.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X1, mRNA.

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CloneID	OXa53315
Clone ID Related Accession (Same CDS sequence)	XM_012966143.3
Accession Version	XM_012966143.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966143.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT 
GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG 
TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT 
AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC 
CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC 
ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC 
TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA 
TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC 
CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG 
GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC 
CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821597.1
CDS	235..4590
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPGVTIPTSPTPSLTRATAHNVAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTDPSDVDVFVVHAPYTPVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966143.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966143.3

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT 
GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG 
TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT 
AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC 
CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC 
ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC 
TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA 
TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC 
CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG 
GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC 
CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966144.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821598.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X2, mRNA.

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CloneID	OXa53316
Clone ID Related Accession (Same CDS sequence)	XM_012966144.3
Accession Version	XM_012966144.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966144.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGC ATCCAGCATC TCTGGGAAAG GAGTTCGCCC TTCTGATCCT 
GGATCAAATG GTGGCTCAAG TCTTGGAGGC AGTAATAGTC ACCATGGGAA CCCAACGTCA 
CTGGATCAGA ACATGCTTTT AACAATTATT GTTTCCATTG GTGTGATCAC TGTCTTGATT 
GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC CGTTCTAGCT CCCACCACAA AAAAAAGAGA 
GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT AAATACAAAG GCAACTCCAA GGATGTGAAA 
CCACCAGACC TGTGGATCCA TCATGAACGT TTGGAATTGA AACCAACAGA CAAGTCCCCA 
GAAACAAATC CTATTTTGAC AGACACGCCT CTGCCACGCT CCCCACAAGA TCTCACACCA 
GGGGATAGCA CCACGGAAAG CAGCATCCAC CAGCACAGCA ATTCGTATCG AGGTCTGGAA 
TCAGAAGATT CTGTGTCCTC ACTTGCTGGG CGCCGTGGCA TGCGACCTAA GATAATGATG 
CCTTTTGACT CCCAGCCTCC ACAACCGGTT GTCAGTGCCC ATCCCATCCA TTCCCACGAT 
AATTCTCATC ATTTTCGCTC CGTTCCTGGG TTCATGCTGC ACGTGTTGGG TGGCCCTCCA 
CAGGCTGCAG AATCTGTGCG TCATACCCCT AGCTGTGACA CTGTAGTAGC TGCATCATCT 
TCCTCTCAGA CTGGCCCTGA AGATCCAGAC CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC 
CTGGATGAAG ATTCAGTACC TAGTGTTCCC ACTGCACATA TCCGTCCTTC TCATCCCCTA 
AAAAGCTTTG CTGTTCCAGC TGTGCCCACC TCTGCTTCTG GAGCTTCGTA TGAACCCTCA 
TTACCTAGCT CTCCGCTTCT TCAACAACAA TGCCCCACCG CTCTGTCTGT AAAGACAGCC 
TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC CCTCTTCCAG TCACAGTTCC CAGTGCCCCT 
GAACCACTAG AGACAAGCAG GATGCTAGAG GGCTCAGAGT GTAGCTATGA ACCGGACGAG 
TTAAGCAAAG AGATGGCTCA CCTTGAAGGC CTGATGAAGG ATCTAAATGC CATTACCACA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821598.1
CDS	235..4560
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPGVTIPTSPTPSLTRATAHNVAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTDPSDVDVFVVHAPYTPVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966144.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966144.3

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGC ATCCAGCATC TCTGGGAAAG GAGTTCGCCC TTCTGATCCT 
GGATCAAATG GTGGCTCAAG TCTTGGAGGC AGTAATAGTC ACCATGGGAA CCCAACGTCA 
CTGGATCAGA ACATGCTTTT AACAATTATT GTTTCCATTG GTGTGATCAC TGTCTTGATT 
GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC CGTTCTAGCT CCCACCACAA AAAAAAGAGA 
GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT AAATACAAAG GCAACTCCAA GGATGTGAAA 
CCACCAGACC TGTGGATCCA TCATGAACGT TTGGAATTGA AACCAACAGA CAAGTCCCCA 
GAAACAAATC CTATTTTGAC AGACACGCCT CTGCCACGCT CCCCACAAGA TCTCACACCA 
GGGGATAGCA CCACGGAAAG CAGCATCCAC CAGCACAGCA ATTCGTATCG AGGTCTGGAA 
TCAGAAGATT CTGTGTCCTC ACTTGCTGGG CGCCGTGGCA TGCGACCTAA GATAATGATG 
CCTTTTGACT CCCAGCCTCC ACAACCGGTT GTCAGTGCCC ATCCCATCCA TTCCCACGAT 
AATTCTCATC ATTTTCGCTC CGTTCCTGGG TTCATGCTGC ACGTGTTGGG TGGCCCTCCA 
CAGGCTGCAG AATCTGTGCG TCATACCCCT AGCTGTGACA CTGTAGTAGC TGCATCATCT 
TCCTCTCAGA CTGGCCCTGA AGATCCAGAC CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC 
CTGGATGAAG ATTCAGTACC TAGTGTTCCC ACTGCACATA TCCGTCCTTC TCATCCCCTA 
AAAAGCTTTG CTGTTCCAGC TGTGCCCACC TCTGCTTCTG GAGCTTCGTA TGAACCCTCA 
TTACCTAGCT CTCCGCTTCT TCAACAACAA TGCCCCACCG CTCTGTCTGT AAAGACAGCC 
TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC CCTCTTCCAG TCACAGTTCC CAGTGCCCCT 
GAACCACTAG AGACAAGCAG GATGCTAGAG GGCTCAGAGT GTAGCTATGA ACCGGACGAG 
TTAAGCAAAG AGATGGCTCA CCTTGAAGGC CTGATGAAGG ATCTAAATGC CATTACCACA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966146.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821600.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa53317
Clone ID Related Accession (Same CDS sequence)	XM_012966146.3
Accession Version	XM_012966146.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966146.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT 
GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG 
TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT 
AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC 
CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC 
ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC 
TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA 
TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC 
CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG 
GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC 
CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821600.1
CDS	235..4530
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTDPSDVDVFVVHAPYTPVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966146.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966146.3

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACCGGTT 
GTCAGTGCCC ATCCCATCCA TTCCCACGAT AATTCTCATC ATTTTCGCTC CGTTCCTGGG 
TTCATGCTGC ACGTGTTGGG TGGCCCTCCA CAGGCTGCAG AATCTGTGCG TCATACCCCT 
AGCTGTGACA CTGTAGTAGC TGCATCATCT TCCTCTCAGA CTGGCCCTGA AGATCCAGAC 
CCCTCGGGGG TTAGTTTCTT GCCTGGCTCC CTGGATGAAG ATTCAGTACC TAGTGTTCCC 
ACTGCACATA TCCGTCCTTC TCATCCCCTA AAAAGCTTTG CTGTTCCAGC TGTGCCCACC 
TCTGCTTCTG GAGCTTCGTA TGAACCCTCA TTACCTAGCT CTCCGCTTCT TCAACAACAA 
TGCCCCACCG CTCTGTCTGT AAAGACAGCC TCTCTTGGAA CTCTTGGCAG GGTGCGCCCC 
CCTCTTCCAG TCACAGTTCC CAGTGCCCCT GAACCACTAG AGACAAGCAG GATGCTAGAG 
GGCTCAGAGT GTAGCTATGA ACCGGACGAG TTAAGCAAAG AGATGGCTCA CCTTGAAGGC 
CTGATGAAGG ATCTAAATGC CATTACCACA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966147.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821601.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa53318
Clone ID Related Accession (Same CDS sequence)	XM_012966147.3
Accession Version	XM_012966147.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966147.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACCACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC 
CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG 
AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC 
ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC 
AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC 
AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA 
GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA 
GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT 
CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT 
AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC 
CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG 
GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA 
GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC 
TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT 
TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG 
TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT 
GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT 
CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT 
GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT 
GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821601.1
CDS	235..4512
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPGVTIPTSPTPSLTRATAHNVAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966147.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966147.3

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACCACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC 
CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG 
AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC 
ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC 
AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC 
AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA 
GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA 
GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT 
CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT 
AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC 
CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG 
GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA 
GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC 
TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT 
TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG 
TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT 
GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT 
CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT 
GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT 
GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966148.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821602.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X5, mRNA.

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CloneID	OXa53319
Clone ID Related Accession (Same CDS sequence)	XM_012966148.3
Accession Version	XM_012966148.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT 
GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC 
CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA 
GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT 
CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG 
CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT 
GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA 
AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG 
GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821602.1
CDS	236..4486
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPGVTIPTSPTPSLTRATAHNVAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTDPSDVDVFVVHAPYTPVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQQSVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966148.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966148.3

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGGTGTTAC CATCCCAACC TCTCCCACCC CATCACTGAC CCGTGCCACT 
GCTCACAATG TAGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT 
GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC 
CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA 
GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT 
CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG 
CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT 
GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA 
AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG 
GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966149.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821603.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X6, mRNA.

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CloneID	OXa53320
Clone ID Related Accession (Same CDS sequence)	XM_012966149.3
Accession Version	XM_012966149.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966149.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACCACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA 
GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA 
AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT 
TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC 
ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG 
AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC 
CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG 
ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA 
AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC 
TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT 
CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC 
TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG 
CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT 
GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA 
CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA 
GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT 
CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC 
AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC 
AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT 
CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821603.1
CDS	235..4482
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966149.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966149.3

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACCACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GAGTCATCAC CTTACATGCC TAACGACCAA 
GCATCCAGCA TCTCTGGGAA AGGAGTTCGC CCTTCTGATC CTGGATCAAA TGGTGGCTCA 
AGTCTTGGAG GCAGTAATAG TCACCATGGG AACCCAACGT CACTGGATCA GAACATGCTT 
TTAACAATTA TTGTTTCCAT TGGTGTGATC ACTGTCTTGA TTGCTGTGGT CATAGCAGCC 
ATATGTTCCC GCCGTTCTAG CTCCCACCAC AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG 
AATGGGTCGC ATAAATACAA AGGCAACTCC AAGGATGTGA AACCACCAGA CCTGTGGATC 
CATCATGAAC GTTTGGAATT GAAACCAACA GACAAGTCCC CAGAAACAAA TCCTATTTTG 
ACAGACACGC CTCTGCCACG CTCCCCACAA GATCTCACAC CAGGGGATAG CACCACGGAA 
AGCAGCATCC ACCAGCACAG CAATTCGTAT CGAGGTCTGG AATCAGAAGA TTCTGTGTCC 
TCACTTGCTG GGCGCCGTGG CATGCGACCT AAGATAATGA TGCCTTTTGA CTCCCAGCCT 
CCACAACCGG TTGTCAGTGC CCATCCCATC CATTCCCACG ATAATTCTCA TCATTTTCGC 
TCCGTTCCTG GGTTCATGCT GCACGTGTTG GGTGGCCCTC CACAGGCTGC AGAATCTGTG 
CGTCATACCC CTAGCTGTGA CACTGTAGTA GCTGCATCAT CTTCCTCTCA GACTGGCCCT 
GAAGATCCAG ACCCCTCGGG GGTTAGTTTC TTGCCTGGCT CCCTGGATGA AGATTCAGTA 
CCTAGTGTTC CCACTGCACA TATCCGTCCT TCTCATCCCC TAAAAAGCTT TGCTGTTCCA 
GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG TATGAACCCT CATTACCTAG CTCTCCGCTT 
CTTCAACAAC AATGCCCCAC CGCTCTGTCT GTAAAGACAG CCTCTCTTGG AACTCTTGGC 
AGGGTGCGCC CCCCTCTTCC AGTCACAGTT CCCAGTGCCC CTGAACCACT AGAGACAAGC 
AGGATGCTAG AGGGCTCAGA GTGTAGCTAT GAACCGGACG AGTTAAGCAA AGAGATGGCT 
CACCTTGAAG GCCTGATGAA GGATCTAAAT GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966150.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821604.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X7, mRNA.

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CloneID	OXa53321
Clone ID Related Accession (Same CDS sequence)	XM_012966150.3
Accession Version	XM_012966150.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966150.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACCACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC 
CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG 
AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC 
ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC 
AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC 
AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA 
GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA 
GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT 
CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT 
AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC 
CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG 
GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA 
GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC 
TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT 
TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG 
TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT 
GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT 
CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT 
GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT 
GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821604.1
CDS	235..4452
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQPVVSAHPIHSHDNSHHFRSVPGFMLHVLGGPPQAAESVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966150.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966150.3

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGTTCCAGA GCCCAGTCCT ATGTTGCCCC CAGTTGGCGT GCAAGCCACT 
ATCGTGGCCA GTGACACTGT GCGTCTCACA TGGGCGGATA ACTCTCTCCC CAAGAACCAG 
AAGATCACTG ATTCAAGATA TTACACAGTG AGATGGAAGA CCAATATTCC AGCAAATACC 
AAATACAAGA TGGCAAACAG CACAACCTTA AGTTACTTGG TTACTGGTCT GAAGCCCAAC 
ACTTTATATG AGTTCTCAGT CATGGTGACC AAAGGTCGTA GATCAAGTAC ATGGAGTATG 
ACAGCACATG GGACCACATT TGAATCAGTG CCTTCCTCAC CTCCCAAAGA TGTAACTGTT 
GTGAGCAAGG AAGGAAAACC TCGCACCATT ATTGTCAACT GGCAGCCTCC CTCAGAAGCC 
AATGGTAAAA TAACAGGTTA TATTATATAT TACAGTACAG ATGCTCAGGC TGAACTCCAT 
GACTGGGTGA TTGAGCCTGT GATTGGAAAC CGTCTCACCC ACCAGATACA AGAGTTGACT 
CTAGACACCC CTTATTATTT TAAGATCCAG GCAAGAAATT CTAAGGGCAT GGGTCCTATG 
TCAGAAGCTG TGCTTTTCCG AACACCAAAG GCATCCAGCA TCTCTGGGAA AGGAGTTCGC 
CCTTCTGATC CTGGATCAAA TGGTGGCTCA AGTCTTGGAG GCAGTAATAG TCACCATGGG 
AACCCAACGT CACTGGATCA GAACATGCTT TTAACAATTA TTGTTTCCAT TGGTGTGATC 
ACTGTCTTGA TTGCTGTGGT CATAGCAGCC ATATGTTCCC GCCGTTCTAG CTCCCACCAC 
AAAAAAAAGA GAGCTGCCAG TAAGTCAGTG AATGGGTCGC ATAAATACAA AGGCAACTCC 
AAGGATGTGA AACCACCAGA CCTGTGGATC CATCATGAAC GTTTGGAATT GAAACCAACA 
GACAAGTCCC CAGAAACAAA TCCTATTTTG ACAGACACGC CTCTGCCACG CTCCCCACAA 
GATCTCACAC CAGGGGATAG CACCACGGAA AGCAGCATCC ACCAGCACAG CAATTCGTAT 
CGAGGTCTGG AATCAGAAGA TTCTGTGTCC TCACTTGCTG GGCGCCGTGG CATGCGACCT 
AAGATAATGA TGCCTTTTGA CTCCCAGCCT CCACAACCGG TTGTCAGTGC CCATCCCATC 
CATTCCCACG ATAATTCTCA TCATTTTCGC TCCGTTCCTG GGTTCATGCT GCACGTGTTG 
GGTGGCCCTC CACAGGCTGC AGAATCTGTG CGTCATACCC CTAGCTGTGA CACTGTAGTA 
GCTGCATCAT CTTCCTCTCA GACTGGCCCT GAAGATCCAG ACCCCTCGGG GGTTAGTTTC 
TTGCCTGGCT CCCTGGATGA AGATTCAGTA CCTAGTGTTC CCACTGCACA TATCCGTCCT 
TCTCATCCCC TAAAAAGCTT TGCTGTTCCA GCTGTGCCCA CCTCTGCTTC TGGAGCTTCG 
TATGAACCCT CATTACCTAG CTCTCCGCTT CTTCAACAAC AATGCCCCAC CGCTCTGTCT 
GTAAAGACAG CCTCTCTTGG AACTCTTGGC AGGGTGCGCC CCCCTCTTCC AGTCACAGTT 
CCCAGTGCCC CTGAACCACT AGAGACAAGC AGGATGCTAG AGGGCTCAGA GTGTAGCTAT 
GAACCGGACG AGTTAAGCAA AGAGATGGCT CACCTTGAAG GCCTGATGAA GGATCTAAAT 
GCCATTACCA CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

neo1 ( XM_012966151.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821605.1 Xenopus tropicalis neogenin 1 (neo1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa53322
Clone ID Related Accession (Same CDS sequence)	XM_012966151.3
Accession Version	XM_012966151.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neogenin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966151.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT 
GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC 
CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA 
GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT 
CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG 
CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT 
GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA 
AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG 
GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821605.1
CDS	236..4426
Translation	MAIRSPPGSLVRVTRGLFAVSILFSVWKKPTWAAKTAGSGFRTFTPFYFLTEPVDTVTLRGFAAVLNCSAYADPTPKIEWKKDGTFLNLVSDDRRRLLPDGSLLITSVVHSKHNKPDEGIYQCVATVESLGSIVSRTARLSVAGLPRFTSQPESSSVYVGDSIVLNCDVSPELVPFVHWEQNKASRELDNRVAVLLNGSLVISNANESDAGLYRCAVGGGSSIKYSEEAEIRVLQDTGDERPMLFLRQPLPLTRVSGQTALLTCVVSGYPTPSVRWTHNQKEILTENSETLTFVAGGSLQISSVTEEDSGIYTCIADNGNQTIQAHAELSIQVPPIFHVKPSNTHAHESMDIVFKCEVTGKPTPTVKWVKNGDMVIPSDYFKIVEDHDLQVLGLVRSDEGFYQCIAENEVGNVQAAAQLIILEPAATPGGPLPSSPRDVVASLVSTRFIKLTWRMPADPHGENLTYQVYYSKESMNRERVENTSRPGEMQVTIQNLLPETVYYFRVVAQNQNGAGESSLPLKVETQPEVQVPGPAPNFKATPVSPTSVSVSWETPLSGNGEIQSYKLYYMEKGADNEQDVDVGGHSYTLNGLKKYTEYTFRVVAFNKHGPGVSTQDVTVRTLSDVPSAAPQNLTLEVRSSQSILVQWQPPPIGSQNGQIVGYKVRYRKTTRKSETSEILVDAQLSYLFTALERDTEYSFRAVALTVNGSGPASDWVSAETFESDLDESRVPDVPSSLHVRPLVTNIVVSWTPPENQHQVVVRGYAIGYGLGSPHAQTVRVDHKQRYYTIDNLEPSSHYVISLRAFNNVGEGIPLYESAVTRQHTDPSDVDVFVVHAPYTPVPEPSPMLPPVGVQATIVASDTVRLTWADNSLPKNQKITDSRYYTVRWKTNIPANTKYKMANSTTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFESVPSSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDAQAELHDWVIEPVIGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVLFRTPKESSPYMPNDQASSISGKGVRPSDPGSNGGSSLGGSNSHHGNPTSLDQNMLLTIIVSIGVITVLIAVVIAAICSRRSSSHHKKKRAASKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPTDKSPETNPILTDTPLPRSPQDLTPGDSTTESSIHQHSNSYRGLESEDSVSSLAGRRGMRPKIMMPFDSQPPQQSVRHTPSCDTVVAASSSSQTGPEDPDPSGVSFLPGSLDEDSVPSVPTAHIRPSHPLKSFAVPAVPTSASGASYEPSLPSSPLLQQQCPTALSVKTASLGTLGRVRPPLPVTVPSAPEPLETSRMLEGSECSYEPDELSKEMAHLEGLMKDLNAITTA

Target ORF information:

RefSeq Version	XM_012966151.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis neogenin 1 (neo1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966151.3

ORF Insert Sequence:

ATGGCGATCC GAAGCCCCCC GGGTTCGCTT GTTCGGGTCA CCCGGGGGCT GTTCGCCGTC 
TCCATCCTTT TTTCCGTGTG GAAGAAGCCG ACTTGGGCTG CCAAGACTGC AGGCTCAGGA 
TTCAGAACCT TTACACCCTT TTATTTCCTG ACGGAGCCAG TGGACACAGT GACTTTGCGG 
GGTTTTGCTG CAGTACTAAA CTGCTCTGCT TATGCGGATC CAACGCCGAA GATTGAATGG 
AAAAAGGATG GGACATTTTT GAATCTTGTG TCTGATGATC GCCGCAGGCT CTTACCAGAT 
GGGTCATTGC TTATAACAAG TGTCGTGCAC TCAAAGCACA ACAAGCCAGA TGAAGGCATC 
TACCAGTGTG TGGCCACTGT AGAAAGTCTG GGAAGCATAG TCAGCCGCAC TGCCCGACTC 
TCTGTTGCAG GTCTCCCTCG ATTTACTAGC CAGCCAGAGT CCTCTTCTGT ATATGTTGGG 
GATAGCATTG TATTGAACTG TGATGTCAGT CCTGAACTGG TGCCTTTTGT GCATTGGGAG 
CAAAACAAGG CTTCACGAGA ATTGGACAAC AGAGTAGCTG TCCTGTTAAA TGGCAGCTTG 
GTCATTAGCA ATGCCAATGA AAGTGATGCT GGGCTATACC GCTGTGCAGT AGGTGGTGGG 
TCATCCATTA AATACAGTGA GGAGGCAGAG ATTAGAGTAC TTCAAGACAC AGGAGACGAG 
CGCCCCATGC TGTTTCTTCG CCAGCCTTTG CCACTTACCA GAGTTTCTGG GCAGACTGCA 
CTCTTGACTT GTGTGGTATC TGGGTATCCA ACACCCAGTG TGCGCTGGAC CCATAACCAG 
AAAGAAATAC TGACTGAAAA CTCAGAGACG TTAACATTCG TGGCAGGGGG CAGCCTGCAG 
ATCAGTTCTG TAACGGAAGA AGATTCTGGA ATTTACACAT GCATTGCCGA CAATGGGAAC 
CAGACTATTC AGGCTCATGC TGAGCTTAGC ATACAAGTAC CGCCTATTTT CCATGTCAAG 
CCATCCAATA CTCATGCACA CGAGTCCATG GACATTGTAT TTAAGTGTGA GGTAACCGGG 
AAACCGACAC CCACAGTTAA ATGGGTTAAA AATGGAGATA TGGTGATTCC AAGTGACTAC 
TTCAAAATAG TGGAGGACCA TGACCTGCAG GTGCTTGGTC TGGTGCGCTC AGATGAGGGA 
TTCTATCAGT GTATAGCTGA AAATGAGGTG GGAAACGTCC AGGCAGCAGC ACAGCTCATT 
ATCCTTGAGC CTGCTGCCAC TCCAGGAGGC CCCCTGCCTT CATCCCCAAG GGATGTCGTA 
GCCTCTCTGG TGTCTACACG TTTCATAAAG CTGACTTGGC GTATGCCGGC TGATCCACAT 
GGAGAGAATC TCACCTATCA GGTCTACTAT AGCAAGGAAA GCATGAATAG GGAGCGAGTG 
GAGAACACGA GCCGCCCAGG AGAAATGCAA GTTACCATCC AGAACCTTCT ACCCGAGACA 
GTCTATTACT TTCGAGTGGT GGCTCAAAAT CAGAATGGAG CAGGTGAAAG CTCTCTGCCT 
CTAAAGGTTG AAACACAGCC TGAAGTACAA GTTCCTGGCC CAGCCCCCAA CTTTAAAGCC 
ACACCAGTCT CACCCACATC TGTAAGTGTC TCTTGGGAAA CCCCACTGTC TGGAAATGGG 
GAGATTCAGA GCTACAAGCT TTATTATATG GAAAAAGGAG CAGACAATGA ACAGGATGTT 
GATGTGGGTG GACATTCATA TACATTAAAT GGCCTGAAAA AATATACAGA GTATACCTTC 
AGGGTGGTAG CCTTTAACAA GCATGGGCCA GGTGTATCCA CGCAGGATGT GACTGTCAGG 
ACTCTGTCAG ATGTACCCAG TGCTGCCCCT CAGAACCTGA CACTAGAAGT TCGGAGTTCA 
CAGAGCATTC TAGTGCAGTG GCAGCCCCCT CCTATAGGTT CCCAAAATGG ACAGATTGTG 
GGCTACAAGG TCCGTTACCG CAAGACAACA AGAAAGAGTG AGACTAGTGA GATTCTTGTG 
GATGCTCAGC TATCATATCT TTTCACAGCT CTTGAGCGGG ACACCGAGTA CAGTTTTCGG 
GCTGTGGCCT TGACTGTTAA TGGAAGTGGC CCAGCCTCAG ACTGGGTTTC AGCAGAAACC 
TTTGAGAGTG ATCTGGATGA GTCGCGTGTA CCAGATGTGC CAAGCTCCCT CCATGTACGT 
CCTTTGGTCA CAAACATTGT AGTGAGCTGG ACCCCCCCAG AAAACCAGCA CCAGGTGGTG 
GTTCGTGGAT ACGCAATTGG CTATGGCTTA GGAAGTCCAC ATGCCCAGAC TGTACGTGTG 
GACCACAAGC AGCGTTACTA TACCATTGAT AACCTTGAAC CCAGCTCCCA CTATGTTATC 
TCGCTTAGAG CTTTTAACAA TGTTGGAGAG GGAATTCCAC TGTATGAGAG TGCAGTCACC 
CGGCAGCACA CAGACCCTTC CGATGTTGAT GTGTTTGTTG TTCATGCCCC ATACACCCCA 
GTTCCAGAGC CCAGTCCTAT GTTGCCCCCA GTTGGCGTGC AAGCCACTAT CGTGGCCAGT 
GACACTGTGC GTCTCACATG GGCGGATAAC TCTCTCCCCA AGAACCAGAA GATCACTGAT 
TCAAGATATT ACACAGTGAG ATGGAAGACC AATATTCCAG CAAATACCAA ATACAAGATG 
GCAAACAGCA CAACCTTAAG TTACTTGGTT ACTGGTCTGA AGCCCAACAC TTTATATGAG 
TTCTCAGTCA TGGTGACCAA AGGTCGTAGA TCAAGTACAT GGAGTATGAC AGCACATGGG 
ACCACATTTG AATCAGTGCC TTCCTCACCT CCCAAAGATG TAACTGTTGT GAGCAAGGAA 
GGAAAACCTC GCACCATTAT TGTCAACTGG CAGCCTCCCT CAGAAGCCAA TGGTAAAATA 
ACAGGTTATA TTATATATTA CAGTACAGAT GCTCAGGCTG AACTCCATGA CTGGGTGATT 
GAGCCTGTGA TTGGAAACCG TCTCACCCAC CAGATACAAG AGTTGACTCT AGACACCCCT 
TATTATTTTA AGATCCAGGC AAGAAATTCT AAGGGCATGG GTCCTATGTC AGAAGCTGTG 
CTTTTCCGAA CACCAAAGGA GTCATCACCT TACATGCCTA ACGACCAAGC ATCCAGCATC 
TCTGGGAAAG GAGTTCGCCC TTCTGATCCT GGATCAAATG GTGGCTCAAG TCTTGGAGGC 
AGTAATAGTC ACCATGGGAA CCCAACGTCA CTGGATCAGA ACATGCTTTT AACAATTATT 
GTTTCCATTG GTGTGATCAC TGTCTTGATT GCTGTGGTCA TAGCAGCCAT ATGTTCCCGC 
CGTTCTAGCT CCCACCACAA AAAAAAGAGA GCTGCCAGTA AGTCAGTGAA TGGGTCGCAT 
AAATACAAAG GCAACTCCAA GGATGTGAAA CCACCAGACC TGTGGATCCA TCATGAACGT 
TTGGAATTGA AACCAACAGA CAAGTCCCCA GAAACAAATC CTATTTTGAC AGACACGCCT 
CTGCCACGCT CCCCACAAGA TCTCACACCA GGGGATAGCA CCACGGAAAG CAGCATCCAC 
CAGCACAGCA ATTCGTATCG AGGTCTGGAA TCAGAAGATT CTGTGTCCTC ACTTGCTGGG 
CGCCGTGGCA TGCGACCTAA GATAATGATG CCTTTTGACT CCCAGCCTCC ACAACAATCT 
GTGCGTCATA CCCCTAGCTG TGACACTGTA GTAGCTGCAT CATCTTCCTC TCAGACTGGC 
CCTGAAGATC CAGACCCCTC GGGGGTTAGT TTCTTGCCTG GCTCCCTGGA TGAAGATTCA 
GTACCTAGTG TTCCCACTGC ACATATCCGT CCTTCTCATC CCCTAAAAAG CTTTGCTGTT 
CCAGCTGTGC CCACCTCTGC TTCTGGAGCT TCGTATGAAC CCTCATTACC TAGCTCTCCG 
CTTCTTCAAC AACAATGCCC CACCGCTCTG TCTGTAAAGA CAGCCTCTCT TGGAACTCTT 
GGCAGGGTGC GCCCCCCTCT TCCAGTCACA GTTCCCAGTG CCCCTGAACC ACTAGAGACA 
AGCAGGATGC TAGAGGGCTC AGAGTGTAGC TATGAACCGG ACGAGTTAAG CAAAGAGATG 
GCTCACCTTG AAGGCCTGAT GAAGGATCTA AATGCCATTA CCACAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	neo1
Entrez Gene ID	100170189
Full Name	neogenin 1
Synonyms	hst17534,neogenin,ngn
General protein information	Preferred Names neogenin 1 Names neogenin neogenin homolog 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

neo1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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