trdn cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	trdn
Entrez Gene ID	100145422
Full Name	triadin
Synonyms	tdn,trisk,trisk51
General protein information	Preferred Names triadin Names triadin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012963627.2	XP_012819081.1	triadin isoform X15
XM_012963633.2	XP_012819087.1	triadin isoform X23
XM_012963639.2	XP_012819093.1	triadin isoform X27
XM_012963649.2	XP_012819103.1	triadin isoform X37
XM_012963612.2	XP_012819066.1	triadin isoform X3
XM_012963615.2	XP_012819069.1	triadin isoform X5
XM_012963621.2	XP_012819075.1	triadin isoform X10
XM_031902068.1	XP_031757928.1	triadin isoform X3
XM_031902072.1	XP_031757932.1	triadin isoform X7
XM_031902074.1	XP_031757934.1	triadin isoform X9
XM_031902075.1	XP_031757935.1	triadin isoform X10
XM_031902081.1	XP_031757941.1	triadin isoform X16
XM_031902087.1	XP_031757947.1	triadin isoform X21
XM_031902094.1	XP_031757954.1	triadin isoform X28
XM_031902095.1	XP_031757955.1	triadin isoform X29
XM_031902098.1	XP_031757958.1	triadin isoform X32
XM_031902101.1	XP_031757961.1	triadin isoform X35
XM_031902102.1	XP_031757962.1	triadin isoform X36
XM_031902112.1	XP_031757972.1	triadin isoform X46
XM_031902115.1	XP_031757975.1	triadin isoform X49
XM_012963616.2	XP_012819070.1	triadin isoform X6
XM_012963618.2	XP_012819072.1	triadin isoform X8
XM_012963620.2	XP_012819074.1	triadin isoform X9
XM_012963624.2	XP_012819078.1	triadin isoform X16
XM_012963630.2	XP_012819084.1	triadin isoform X18
XM_012963631.2	XP_012819085.1	triadin isoform X19
XM_012963638.2	XP_012819092.1	triadin isoform X26
XM_012963642.2	XP_012819096.1	triadin isoform X28
XM_012963643.2	XP_012819097.1	triadin isoform X29
XM_012963640.2	XP_012819094.1	triadin isoform X31
XM_012963653.2	XP_012819107.1	triadin isoform X41
XM_012963656.2	XP_012819110.1	triadin isoform X42
XM_012963660.2	XP_012819114.1	triadin isoform X47
XM_031902067.1	XP_031757927.1	triadin isoform X2
XM_031902069.1	XP_031757929.1	triadin isoform X4
XM_031902085.1	XP_031757945.1	triadin isoform X19
XM_031902088.1	XP_031757948.1	triadin isoform X22
XM_031902092.1	XP_031757952.1	triadin isoform X26
XM_031902104.1	XP_031757964.1	triadin isoform X38
XM_031902105.1	XP_031757965.1	triadin isoform X39
XM_031902108.1	XP_031757968.1	triadin isoform X42
XM_031902116.1	XP_031757976.1	triadin isoform X50
XM_012963657.2	XP_012819111.1	triadin isoform X44
XM_012963622.2	XP_012819076.1	triadin isoform X11
XM_012963623.2	XP_012819077.1	triadin isoform X12
XM_012963625.2	XP_012819079.1	triadin isoform X13
XM_012963632.2	XP_012819086.1	triadin isoform X20
XM_012963637.2	XP_012819091.1	triadin isoform X25
XM_012963645.2	XP_012819099.1	triadin isoform X33
XM_012963646.2	XP_012819100.1	triadin isoform X34
XM_012963648.2	XP_012819102.1	triadin isoform X36
XM_012963655.2	XP_012819109.1	triadin isoform X43
XM_031902066.1	XP_031757926.1	triadin isoform X1
XM_031902070.1	XP_031757930.1	triadin isoform X5
XM_031902076.1	XP_031757936.1	triadin isoform X11
XM_031902077.1	XP_031757937.1	triadin isoform X12
XM_031902078.1	XP_031757938.1	triadin isoform X13
XM_031902080.1	XP_031757940.1	triadin isoform X15
XM_031902084.1	XP_031757944.1	triadin isoform X18
XM_031902097.1	XP_031757957.1	triadin isoform X31
XM_031902114.1	XP_031757974.1	triadin isoform X48
XM_012963610.2	XP_012819064.1	triadin isoform X2
XM_012963613.2	XP_012819067.1	triadin isoform X4
XM_012963617.2	XP_012819071.1	triadin isoform X7
XM_012963626.2	XP_012819080.1	triadin isoform X14
XM_012963644.2	XP_012819098.1	triadin isoform X30
XM_012963654.2	XP_012819108.1	triadin isoform X40
XM_012963658.2	XP_012819112.1	triadin isoform X45
XM_031902082.1	XP_031757942.1	triadin isoform X17
XM_031902089.1	XP_031757949.1	triadin isoform X23
XM_031902090.1	XP_031757950.1	triadin isoform X24
XM_031902093.1	XP_031757953.1	triadin isoform X27
XM_031902099.1	XP_031757959.1	triadin isoform X33
XM_031902103.1	XP_031757963.1	triadin isoform X37
XM_031902106.1	XP_031757966.1	triadin isoform X40
XM_031902107.1	XP_031757967.1	triadin isoform X41
XM_031902110.1	XP_031757970.1	triadin isoform X44
XM_031902113.1	XP_031757973.1	triadin isoform X47
XM_012963629.2	XP_012819083.1	triadin isoform X17
XM_018094229.1	XP_017949718.1	triadin isoform X21
XM_018094230.1	XP_017949719.1	triadin isoform X22
XM_012963635.2	XP_012819089.1	triadin isoform X24
XM_018094231.1	XP_017949720.1	triadin isoform X32
XM_012963647.2	XP_012819101.1	triadin isoform X35
XM_012963650.2	XP_012819104.1	triadin isoform X38
XM_012963651.2	XP_012819105.1	triadin isoform X39
XM_018094232.1	XP_017949721.1	triadin isoform X46
XM_012963609.2	XP_012819063.1	triadin isoform X1
XM_031902071.1	XP_031757931.1	triadin isoform X6
XM_031902073.1	XP_031757933.1	triadin isoform X8
XM_031902079.1	XP_031757939.1	triadin isoform X14
XM_031902086.1	XP_031757946.1	triadin isoform X20
XM_031902091.1	XP_031757951.1	triadin isoform X25
XM_031902096.1	XP_031757956.1	triadin isoform X30
XM_031902100.1	XP_031757960.1	triadin isoform X34
XM_031902109.1	XP_031757969.1	triadin isoform X43
XM_031902111.1	XP_031757971.1	triadin isoform X45

Pathways from BioSystems
Gene Ontology

The following trdn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trdn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa58364	XM_031902066.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58365	XM_031902067.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58366	XM_031902068.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58367	XM_031902069.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58368	XM_031902070.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58369	XM_031902071.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58370	XM_031902072.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58371	XM_031902073.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58372	XM_031902074.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58373	XM_031902075.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58374	XM_031902076.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58375	XM_031902077.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58376	XM_031902078.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58377	XM_031902079.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58378	XM_031902080.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58379	XM_031902081.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58380	XM_031902082.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58381	XM_031902084.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58382	XM_031902085.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58383	XM_031902086.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58384	XM_031902087.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58385	XM_031902088.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58386	XM_031902089.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58387	XM_031902090.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58388	XM_031902091.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58389	XM_031902092.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58390	XM_031902093.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58391	XM_031902094.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58392	XM_031902095.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58393	XM_031902096.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58394	XM_031902097.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58394	XM_031902098.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58395	XM_031902099.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58396	XM_031902100.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58397	XM_031902101.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58398	XM_031902102.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58399	XM_031902103.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58400	XM_031902104.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58401	XM_031902105.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58402	XM_031902106.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58403	XM_031902107.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58404	XM_031902108.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X42, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58405	XM_031902109.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X43, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58406	XM_031902110.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X44, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58407	XM_031902111.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X45, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58408	XM_031902112.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X46, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58409	XM_031902113.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X47, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58410	XM_031902114.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X48, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58411	XM_031902115.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X49, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa58412	XM_031902116.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X50, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa31743	XM_012963609.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31744	XM_012963610.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31746	XM_012963612.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31747	XM_012963613.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31749	XM_012963615.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31750	XM_012963616.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31751	XM_012963617.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31752	XM_012963618.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31754	XM_012963620.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31755	XM_012963621.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31756	XM_012963622.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31757	XM_012963623.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31758	XM_012963624.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31759	XM_012963625.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31760	XM_012963626.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31761	XM_012963627.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31763	XM_012963629.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31764	XM_012963630.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31765	XM_012963631.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31766	XM_012963632.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31767	XM_012963633.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31769	XM_012963635.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31771	XM_012963637.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31772	XM_012963638.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31773	XM_012963639.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31774	XM_012963640.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31775	XM_012963642.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31776	XM_012963643.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31777	XM_012963644.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31778	XM_012963645.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31779	XM_012963646.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31780	XM_012963647.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31781	XM_012963648.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31782	XM_012963649.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31783	XM_012963650.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31784	XM_012963651.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31785	XM_012963654.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31785	XM_012963653.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31786	XM_012963655.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X43, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31787	XM_012963656.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X42, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31788	XM_012963657.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X44, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31789	XM_012963658.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X45, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31790	XM_018094232.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X46, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa31791	XM_012963660.2 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X47, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa44647	XM_018094229.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa44648	XM_018094230.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OXa44649	XM_018094231.1 Latest version!	Xenopus tropicalis triadin (trdn), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trdn ( XM_031902066.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757926.1 Xenopus tropicalis triadin (trdn), transcript variant X1, mRNA.

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CloneID	OXa58364
Clone ID Related Accession (Same CDS sequence)	XM_031902066.1
Accession Version	XM_031902066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GTCCGAGCCA AAACCTGCAG AAGTTAAGAA AGATGAACTG 
AAGACACCAA AAGCCATTTA TGAAGAAAAA ACAACCAAAT CGAATGATAC AGCAAATATG 
CTGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757926.1
CDS	383..4711
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902066.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902066.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GTCCGAGCCA AAACCTGCAG AAGTTAAGAA AGATGAACTG 
AAGACACCAA AAGCCATTTA TGAAGAAAAA ACAACCAAAT CGAATGATAC AGCAAATATG 
CTGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902067.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757927.1 Xenopus tropicalis triadin (trdn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa58365
Clone ID Related Accession (Same CDS sequence)	XM_031902067.1
Accession Version	XM_031902067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGATGTAA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GTCCGAGCCA AAACCTGCAG AAGTTAAGAA AGATGAACTG 
AAGACACCAA AAGCCATTTA TGAAGAAAAA ACAACCAAAT CGAATGATAC AGCAAATATG 
CTGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757927.1
CDS	383..4711
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPVKKPEAKPADVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902067.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902067.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGATGTAA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GTCCGAGCCA AAACCTGCAG AAGTTAAGAA AGATGAACTG 
AAGACACCAA AAGCCATTTA TGAAGAAAAA ACAACCAAAT CGAATGATAC AGCAAATATG 
CTGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902068.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757928.1 Xenopus tropicalis triadin (trdn), transcript variant X3, mRNA.

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CloneID	OXa58366
Clone ID Related Accession (Same CDS sequence)	XM_031902068.1
Accession Version	XM_031902068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757928.1
CDS	383..4663
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902068.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902068.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902069.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757929.1 Xenopus tropicalis triadin (trdn), transcript variant X4, mRNA.

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CloneID	OXa58367
Clone ID Related Accession (Same CDS sequence)	XM_031902069.1
Accession Version	XM_031902069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757929.1
CDS	383..4663
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902069.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902069.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902070.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757930.1 Xenopus tropicalis triadin (trdn), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa58368
Clone ID Related Accession (Same CDS sequence)	XM_031902070.1
Accession Version	XM_031902070.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757930.1
CDS	383..4663
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902070.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902070.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902071.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757931.1 Xenopus tropicalis triadin (trdn), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa58369
Clone ID Related Accession (Same CDS sequence)	XM_031902071.1
Accession Version	XM_031902071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757931.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902071.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902071.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902072.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757932.1 Xenopus tropicalis triadin (trdn), transcript variant X7, mRNA.

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CloneID	OXa58370
Clone ID Related Accession (Same CDS sequence)	XM_031902072.1
Accession Version	XM_031902072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757932.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902072.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902072.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902073.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757933.1 Xenopus tropicalis triadin (trdn), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa58371
Clone ID Related Accession (Same CDS sequence)	XM_031902073.1
Accession Version	XM_031902073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757933.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902073.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902073.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902074.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757934.1 Xenopus tropicalis triadin (trdn), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OXa58372
Clone ID Related Accession (Same CDS sequence)	XM_031902074.1
Accession Version	XM_031902074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757934.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902074.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902074.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902075.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757935.1 Xenopus tropicalis triadin (trdn), transcript variant X10, mRNA.

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CloneID	OXa58373
Clone ID Related Accession (Same CDS sequence)	XM_031902075.1
Accession Version	XM_031902075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757935.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902075.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902075.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902076.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757936.1 Xenopus tropicalis triadin (trdn), transcript variant X11, mRNA.

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CloneID	OXa58374
Clone ID Related Accession (Same CDS sequence)	XM_031902076.1
Accession Version	XM_031902076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757936.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902076.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902076.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902077.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757937.1 Xenopus tropicalis triadin (trdn), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OXa58375
Clone ID Related Accession (Same CDS sequence)	XM_031902077.1
Accession Version	XM_031902077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757937.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902077.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902077.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902078.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757938.1 Xenopus tropicalis triadin (trdn), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OXa58376
Clone ID Related Accession (Same CDS sequence)	XM_031902078.1
Accession Version	XM_031902078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757938.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902078.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902078.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902079.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757939.1 Xenopus tropicalis triadin (trdn), transcript variant X14, mRNA.

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CloneID	OXa58377
Clone ID Related Accession (Same CDS sequence)	XM_031902079.1
Accession Version	XM_031902079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757939.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902079.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902079.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902080.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757940.1 Xenopus tropicalis triadin (trdn), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OXa58378
Clone ID Related Accession (Same CDS sequence)	XM_031902080.1
Accession Version	XM_031902080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757940.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902080.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902080.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902081.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757941.1 Xenopus tropicalis triadin (trdn), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OXa58379
Clone ID Related Accession (Same CDS sequence)	XM_031902081.1
Accession Version	XM_031902081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757941.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902081.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902081.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902082.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757942.1 Xenopus tropicalis triadin (trdn), transcript variant X17, mRNA.

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CloneID	OXa58380
Clone ID Related Accession (Same CDS sequence)	XM_031902082.1
Accession Version	XM_031902082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757942.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902082.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902082.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902084.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757944.1 Xenopus tropicalis triadin (trdn), transcript variant X18, mRNA.

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CloneID	OXa58381
Clone ID Related Accession (Same CDS sequence)	XM_031902084.1
Accession Version	XM_031902084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757944.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902084.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902084.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902085.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757945.1 Xenopus tropicalis triadin (trdn), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OXa58382
Clone ID Related Accession (Same CDS sequence)	XM_031902085.1
Accession Version	XM_031902085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757945.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902085.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902085.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902086.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757946.1 Xenopus tropicalis triadin (trdn), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OXa58383
Clone ID Related Accession (Same CDS sequence)	XM_031902086.1
Accession Version	XM_031902086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757946.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902086.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902086.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902087.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757947.1 Xenopus tropicalis triadin (trdn), transcript variant X21, mRNA.

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CloneID	OXa58384
Clone ID Related Accession (Same CDS sequence)	XM_031902087.1
Accession Version	XM_031902087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757947.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902087.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902087.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902088.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757948.1 Xenopus tropicalis triadin (trdn), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OXa58385
Clone ID Related Accession (Same CDS sequence)	XM_031902088.1
Accession Version	XM_031902088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757948.1
CDS	384..4664
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902088.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902088.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGTCCGAGC CAAAACCTGC AGAAGTTAAG 
AAAGATGAAC TGAAGACACC AAAAGCCATT TATGAAGAAA AAACAACCAA ATCGAATGAT 
ACAGCAAATA TGCTGTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902089.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757949.1 Xenopus tropicalis triadin (trdn), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OXa58386
Clone ID Related Accession (Same CDS sequence)	XM_031902089.1
Accession Version	XM_031902089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGCTGA AGTAAAAACA GAAGAGAAGA AAGAAGGAAG AAGCCAACAG 
CCTGCATTTA CCGGTGTATC TACAGATTTT GGCATCATGC CTCGATTGAT CATAATCATG 
TCAGATTCAC ATGGTAGGTT TGCTTCAAGA CCAGATGTCT CTGTAGATTG GACCAAAAGA 
GAAATGTTAC TTAAAAAAGA GGAAAAAGAA GCTGAACCAA TAACACAAGA AAAGATGGAC 
TATATAAAAG AAGCCCCAAC TACATATATA ATAGCAGAGG CTCCTGTGCA GATTAATGTC 
ACAAAACCAC CAGATGCAAA GAAAGAAATA CAAATGGAAA TGCCTGTACG AAAACTTGAA 
GAAGGACCAA AAGAAATAAA AACAGAAATA CCAGTGGAGA TACCTGAAGA AAAAACAAAA 
GATGTAAAAG AAGTTGTAGT AATACCAGTG GAAATGCCAG TGAAGAAAAT TAAACCGGAA 
GAAGAGATAC CAGTGGAAAT GCCAGTGAAA AAACTTAAAG AACCAGAAGA AATACCAGTG 
GAAATACCAG TAGAGAAACC CAAAGAAGTA TCAAAAGCAA AAGAACAAGA GGTGCCACTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCATTCA GAAGACTGAA 
CAAGAAGTGA AGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC CTTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAAAAA 
GAGGAACCAG TTAAGAAGGC TGAACCAGAA GTAAAGGACA TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCTGTAAA GAAACCTGAA 
GCAAAACCTG CAGAAGTAAA GAAAGAGGTG CCCATTAAGA AGACTGAACC AGAAGTGAAG 
GAAGTTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAAAAAGA GGAACCAGTT 
AAGAAGGCTG AACCAGAAGT AAAGGACATT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCTGTAAAGA AACCTGAAGC AAAACCTGCA 
GAAGTAAAGA AAGAGGTGCC CATTAAGAAG ACTGAACCAG AAGTGAAGGA AGTTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGAGGAA CCAGTTAAGA AGGCTGAACC AGAAGTAAAG 
GACATTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGAGGAA CCAGTTAAGA AGGCTGAACC AGAAGTAAAG 
GACATTAAGA AAGAGGTGCC ATTAAAGAAG TCCGAGCCAA AACCTGCAGA AGTTAAGAAA 
GATGAACTGA AGACACCAAA AGCCATTTAT GAAGAAAAAA CAACCAAATC GAATGATACA 
GCAAATATGC TGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757949.1
CDS	383..4660
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902089.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902089.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGCTGA AGTAAAAACA GAAGAGAAGA AAGAAGGAAG AAGCCAACAG 
CCTGCATTTA CCGGTGTATC TACAGATTTT GGCATCATGC CTCGATTGAT CATAATCATG 
TCAGATTCAC ATGGTAGGTT TGCTTCAAGA CCAGATGTCT CTGTAGATTG GACCAAAAGA 
GAAATGTTAC TTAAAAAAGA GGAAAAAGAA GCTGAACCAA TAACACAAGA AAAGATGGAC 
TATATAAAAG AAGCCCCAAC TACATATATA ATAGCAGAGG CTCCTGTGCA GATTAATGTC 
ACAAAACCAC CAGATGCAAA GAAAGAAATA CAAATGGAAA TGCCTGTACG AAAACTTGAA 
GAAGGACCAA AAGAAATAAA AACAGAAATA CCAGTGGAGA TACCTGAAGA AAAAACAAAA 
GATGTAAAAG AAGTTGTAGT AATACCAGTG GAAATGCCAG TGAAGAAAAT TAAACCGGAA 
GAAGAGATAC CAGTGGAAAT GCCAGTGAAA AAACTTAAAG AACCAGAAGA AATACCAGTG 
GAAATACCAG TAGAGAAACC CAAAGAAGTA TCAAAAGCAA AAGAACAAGA GGTGCCACTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCATTCA GAAGACTGAA 
CAAGAAGTGA AGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC CTTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAAAAA 
GAGGAACCAG TTAAGAAGGC TGAACCAGAA GTAAAGGACA TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCTGTAAA GAAACCTGAA 
GCAAAACCTG CAGAAGTAAA GAAAGAGGTG CCCATTAAGA AGACTGAACC AGAAGTGAAG 
GAAGTTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAAAAAGA GGAACCAGTT 
AAGAAGGCTG AACCAGAAGT AAAGGACATT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCTGTAAAGA AACCTGAAGC AAAACCTGCA 
GAAGTAAAGA AAGAGGTGCC CATTAAGAAG ACTGAACCAG AAGTGAAGGA AGTTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGAGGAA CCAGTTAAGA AGGCTGAACC AGAAGTAAAG 
GACATTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGAGGAA CCAGTTAAGA AGGCTGAACC AGAAGTAAAG 
GACATTAAGA AAGAGGTGCC ATTAAAGAAG TCCGAGCCAA AACCTGCAGA AGTTAAGAAA 
GATGAACTGA AGACACCAAA AGCCATTTAT GAAGAAAAAA CAACCAAATC GAATGATACA 
GCAAATATGC TGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902090.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757950.1 Xenopus tropicalis triadin (trdn), transcript variant X24, mRNA.

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CloneID	OXa58387
Clone ID Related Accession (Same CDS sequence)	XM_031902090.1
Accession Version	XM_031902090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGATCATT TAACAGCTCA TAAAGATACA 
GAAGAAAGTC CAAAAAAAGA GCAAGAACCC ATGACACCTC AGATAGTAAA AGATGGACCA 
CCAAAACCAG TTGAAGCTGA AGTAAAAACA GAAGAGAAGA AAGAAGGAAG AAGCCAACAG 
CCTGCATTTA CCGGTGTATC TACAGATTTT GGCATCATGC CTCGATTGAT CATAATCATG 
TCAGATTCAC ATGGTAGGTT TGCTTCAAGA CCAGATGTCT CTGTAGATTG GACCAAAAGA 
GAAATGTTAC TTAAAAAAGA GGAAAAAGAA GCTGAACCAA TAACACAAGA AAAGATGGAC 
TATATAAAAG AAGCCCCAAC TACATATATA ATAGCAGAGG CTCCTGTGCA GATTAATGTC 
ACAAAACCAC CAGATGCAAA GAAAGAAATA CAAATGGAAA TGCCTGTACG AAAACTTGAA 
GAAGGACCAA AAGAAATAAA AACAGAAATA CCAGTGGAGA TACCTGAAGA AAAAACAAAA 
GATGTAAAAG AAGTTGTAGT AATACCAGTG GAAATGCCAG TGAAGAAAAT TAAACCGGAA 
GAAGAGATAC CAGTGGAAAT GCCAGTGAAA AAACTTAAAG AACCAGAAGA AATACCAGTG 
GAAATACCAG TAGAGAAACC CAAAGAAGTA TCAAAAGCAA AAGAACAAGA GGTGCCACTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCATTCA GAAGACTGAA 
CAAGAAGTGA AGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC CTTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAAAAA 
GAGGAACCAG TTAAGAAGGC TGAACCAGAA GTAAAGGACA TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCTGTAAA GAAACCTGAA 
GCAAAACCTG CAGAAGTAAA GAAAGAGGTG CCCATTAAGA AGACTGAACC AGAAGTGAAG 
GAAGTTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAAAAAGA GGAACCAGTT 
AAGAAGGCTG AACCAGAAGT AAAGGACATT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCTGTAAAGA AACCTGAAGC AAAACCTGCA 
GAAGTAAAGA AAGAGGTGCC CATTAAGAAG ACTGAACCAG AAGTGAAGGA AGTTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGAGGAA CCAGTTAAGA AGGCTGAACC AGAAGTAAAG 
GACATTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGAGGAA CCAGTTAAGA AGGCTGAACC AGAAGTAAAG 
GACATTAAGA AAGAGGTGCC ATTAAAGAAG TCCGAGCCAA AACCTGCAGA AGTTAAGAAA 
GATGAACTGA AGACACCAAA AGCCATTTAT GAAGAAAAAA CAACCAAATC GAATGATACA 
GCAAATATGC TGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757950.1
CDS	383..4660
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902090.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902090.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGATCATT TAACAGCTCA TAAAGATACA 
GAAGAAAGTC CAAAAAAAGA GCAAGAACCC ATGACACCTC AGATAGTAAA AGATGGACCA 
CCAAAACCAG TTGAAGCTGA AGTAAAAACA GAAGAGAAGA AAGAAGGAAG AAGCCAACAG 
CCTGCATTTA CCGGTGTATC TACAGATTTT GGCATCATGC CTCGATTGAT CATAATCATG 
TCAGATTCAC ATGGTAGGTT TGCTTCAAGA CCAGATGTCT CTGTAGATTG GACCAAAAGA 
GAAATGTTAC TTAAAAAAGA GGAAAAAGAA GCTGAACCAA TAACACAAGA AAAGATGGAC 
TATATAAAAG AAGCCCCAAC TACATATATA ATAGCAGAGG CTCCTGTGCA GATTAATGTC 
ACAAAACCAC CAGATGCAAA GAAAGAAATA CAAATGGAAA TGCCTGTACG AAAACTTGAA 
GAAGGACCAA AAGAAATAAA AACAGAAATA CCAGTGGAGA TACCTGAAGA AAAAACAAAA 
GATGTAAAAG AAGTTGTAGT AATACCAGTG GAAATGCCAG TGAAGAAAAT TAAACCGGAA 
GAAGAGATAC CAGTGGAAAT GCCAGTGAAA AAACTTAAAG AACCAGAAGA AATACCAGTG 
GAAATACCAG TAGAGAAACC CAAAGAAGTA TCAAAAGCAA AAGAACAAGA GGTGCCACTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCATTCA GAAGACTGAA 
CAAGAAGTGA AGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC CTTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAAAAA 
GAGGAACCAG TTAAGAAGGC TGAACCAGAA GTAAAGGACA TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCTGTAAA GAAACCTGAA 
GCAAAACCTG CAGAAGTAAA GAAAGAGGTG CCCATTAAGA AGACTGAACC AGAAGTGAAG 
GAAGTTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAAAAAGA GGAACCAGTT 
AAGAAGGCTG AACCAGAAGT AAAGGACATT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCTGTAAAGA AACCTGAAGC AAAACCTGCA 
GAAGTAAAGA AAGAGGTGCC CATTAAGAAG ACTGAACCAG AAGTGAAGGA AGTTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGAGGAA CCAGTTAAGA AGGCTGAACC AGAAGTAAAG 
GACATTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGAGGAA CCAGTTAAGA AGGCTGAACC AGAAGTAAAG 
GACATTAAGA AAGAGGTGCC ATTAAAGAAG TCCGAGCCAA AACCTGCAGA AGTTAAGAAA 
GATGAACTGA AGACACCAAA AGCCATTTAT GAAGAAAAAA CAACCAAATC GAATGATACA 
GCAAATATGC TGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902091.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757951.1 Xenopus tropicalis triadin (trdn), transcript variant X25, mRNA.

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CloneID	OXa58388
Clone ID Related Accession (Same CDS sequence)	XM_031902091.1
Accession Version	XM_031902091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGAAGTTCAC TTAACAAGTT CCGGAGTAAT 
CCCGTCGGAA TAATACATGA AGCAGTGGAT GAAACAACTG ATTGGATTTA TGGAATATAT 
ATTACTATAT CTGACTTGAT ATCCCTTGAT GAAGAACGTA AAGGGCATGC TGAGCCTCCT 
GTAAAGAAAA TAGGTGGAAT ACAACCAGCT CTACACAGAA AAGTAATTCA TAAAGAGAAA 
CCTGAAGTAA AAGAGAACAT TTTGAAAAAG GCCTCAGCTA AAGCAATACC AAAACTCAAG 
GCTGAAGAGG AAGATGAGCC AGTTAAGAAA ACCAGAGCAA AAGAAAAGGC TGAAACAAAA 
GAAAAAGTAG AGAAGAAAAT TCTACCTAAA GCCTCACTAA AAGCAGAGAA GAAGGAGAAA 
CCTGAAAAAA TTACCTTGAC AAAATCTGAG AAGAAAGAAA AATCTGCAAT AAAACCAGAG 
AAAAAAGACA AGACGGAAAA GGAAGCCTCA GTCAAAACTG AAAAGAAAGT AAAAGCTGCA 
AAATTAGACA AAGTCAAAAT TGACAAGAAA GAAAAAGCTG AAAAACTTGT CACACCCAAG 
TCTGAGAAGA AGGAAAAAAC TGAAAAAGTA ACCCCATCCA AACTTGAGAA AAAAGTAAAA 
GCTGAACAGA TAGCTCCCAC AAAACTAGAG AAAAAGGAAA AAACTGAAAA GGTAGCAACC 
ACAAAATCAG AAAAGAAAGA AAAAGCTGAA AAGTTAACCC CAGTCAAAGT AGAGAAGACA 
TTAAAACTTG AAAAGGTAGC ACCAGTTAAA GCTGAGAAAA AGGAAAAAGC TGAAAAAGTA 
GTCCCTGTTA AAGTTCAGAA AAAGGAAAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGAAA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGACA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTTCCAGT TAAAGCAGAG 
AAGAAAGGCA AAGCTGAAAA AGTAGCCCCT GTCAAACCCC AGAAGGAGAA AGCTGAAAAA 
GTAACCTCGG TCAAAGCTGA AAAGAAGGAA AAAGCTGAAA AAATTGCCCC AGTAAAAGCC 
GAGAAGAAGG AAAAAGTTGA AAAGATAGCC ACAAGTAAAG CCGAGAAGAA GGTTGAAAAG 
TTAGCCCCAG TCAAACCTGA GAAAAAGGAA AAGGCTAAAA AAGTAGCCCC AGTCAAATCT 
GAGAAGAAGG AAAAGACTGA AAAGTTAACC CAAGACACAA CCGAGAAGGT AAAAGCTGAA 
AAGGTTGCTC CAACCAAATT GGAGAAGAAA CAAAAAGCTG AAACCAGGGC CCCAGTCAAA 
ACTGAGAAGA AGGACAAAGC AGAAAAGGAA ATCATACCAC AAGAAGACAA AAAAGTCAAG 
GTTTCTCAGG TGGAAGAAAA GACTAAGGAT GTCAAAGCTA AAACATCAGA GAGAAAAGTA 
GCAAAAGACT CAAAGAAAGA CGCTAAAGAG GAAGAAGAAA TCAAGACAAA GCCACATACT 
AAAGTGGAAA CACTGGATGC AGCAGAAACA ATGGGGGAAA TAAAAGATCA GTATAATTTC 
TGCCGTTATG TGGTTGACCT GTATTCTCAT GGAGATGCTT CTACAGAAGA AAAATCAAAG 
GAAAAACCAC AAGAGAAGAA AGAAGTTCTC CCACAAGAAA AACCAGGAGA ATTGAAAATA 
TCATTACCAG AAAAGAAAGA TATTCCTGAA ACAAAGGAAA CAGTCTTAGA GGAGGTGACA 
ATTCATGAAG CTGAAGACGA ATGCCCAAAG GAAGATTATG AACAAGTTAC ACCTCAAGCT 
ACTGAAAAAG AACCCCCAAA AGTCATGCAA GATCATTTAA CAGCTCATAA AGATACAGAA 
GAAAGTCCAA AAAAAGAGCA AGAACCCATG ACACCTCAGA TAGTAAAAGA TGGACCACCA 
AAACCAGTTG AAGCTGAAGT AAAAACAGAA GAGAAGAAAG AAGGAAGAAG CCAACAGCCT 
GCATTTACCG GTGTATCTAC AGATTTTGGC ATCATGCCTC GATTGATCAT AATCATGTCA 
GATTCACATG GTAGGTTTGC TTCAAGACCA GATGTCTCTG TAGATTGGAC CAAAAGAGAA 
ATGTTACTTA AAAAAGAGGA AAAAGAAGCT GAACCAATAA CACAAGAAAA GATGGACTAT 
ATAAAAGAAG CCCCAACTAC ATATATAATA GCAGAGGCTC CTGTGCAGAT TAATGTCACA 
AAACCACCAG ATGCAAAGAA AGAAATACAA ATGGAAATGC CTGTACGAAA ACTTGAAGAA 
GGACCAAAAG AAATAAAAAC AGAAATACCA GTGGAGATAC CTGAAGAAAA AACAAAAGAT 
GTAAAAGAAG TTGTAGTAAT ACCAGTGGAA ATGCCAGTGA AGAAAATTAA ACCGGAAGAA 
GAGATACCAG TGGAAATGCC AGTGAAAAAA CTTAAAGAAC CAGAAGAAAT ACCAGTGGAA 
ATACCAGTAG AGAAACCCAA AGAAGTATCA AAAGCAAAAG AACAAGAGGT GCCACTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCATTCAGAA GACTGAACAA 
GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAAAAAGAG 
GAACCAGTTA AGAAGGCTGA ACCAGAAGTA AAGGACATTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA 
AAACCTGCAG AAGTAAAGAA AGAGGTGCCC ATTAAGAAGA CTGAACCAGA AGTGAAGGAA 
GTTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCTGT AAAGAAACCT GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGTCC GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT 
GAACTGAAGA CACCAAAAGC CATTTATGAA GAAAAAACAA CCAAATCGAA TGATACAGCA 
AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757951.1
CDS	383..4657
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902091.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902091.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGAAGTTCAC TTAACAAGTT CCGGAGTAAT 
CCCGTCGGAA TAATACATGA AGCAGTGGAT GAAACAACTG ATTGGATTTA TGGAATATAT 
ATTACTATAT CTGACTTGAT ATCCCTTGAT GAAGAACGTA AAGGGCATGC TGAGCCTCCT 
GTAAAGAAAA TAGGTGGAAT ACAACCAGCT CTACACAGAA AAGTAATTCA TAAAGAGAAA 
CCTGAAGTAA AAGAGAACAT TTTGAAAAAG GCCTCAGCTA AAGCAATACC AAAACTCAAG 
GCTGAAGAGG AAGATGAGCC AGTTAAGAAA ACCAGAGCAA AAGAAAAGGC TGAAACAAAA 
GAAAAAGTAG AGAAGAAAAT TCTACCTAAA GCCTCACTAA AAGCAGAGAA GAAGGAGAAA 
CCTGAAAAAA TTACCTTGAC AAAATCTGAG AAGAAAGAAA AATCTGCAAT AAAACCAGAG 
AAAAAAGACA AGACGGAAAA GGAAGCCTCA GTCAAAACTG AAAAGAAAGT AAAAGCTGCA 
AAATTAGACA AAGTCAAAAT TGACAAGAAA GAAAAAGCTG AAAAACTTGT CACACCCAAG 
TCTGAGAAGA AGGAAAAAAC TGAAAAAGTA ACCCCATCCA AACTTGAGAA AAAAGTAAAA 
GCTGAACAGA TAGCTCCCAC AAAACTAGAG AAAAAGGAAA AAACTGAAAA GGTAGCAACC 
ACAAAATCAG AAAAGAAAGA AAAAGCTGAA AAGTTAACCC CAGTCAAAGT AGAGAAGACA 
TTAAAACTTG AAAAGGTAGC ACCAGTTAAA GCTGAGAAAA AGGAAAAAGC TGAAAAAGTA 
GTCCCTGTTA AAGTTCAGAA AAAGGAAAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGAAA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGACA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTTCCAGT TAAAGCAGAG 
AAGAAAGGCA AAGCTGAAAA AGTAGCCCCT GTCAAACCCC AGAAGGAGAA AGCTGAAAAA 
GTAACCTCGG TCAAAGCTGA AAAGAAGGAA AAAGCTGAAA AAATTGCCCC AGTAAAAGCC 
GAGAAGAAGG AAAAAGTTGA AAAGATAGCC ACAAGTAAAG CCGAGAAGAA GGTTGAAAAG 
TTAGCCCCAG TCAAACCTGA GAAAAAGGAA AAGGCTAAAA AAGTAGCCCC AGTCAAATCT 
GAGAAGAAGG AAAAGACTGA AAAGTTAACC CAAGACACAA CCGAGAAGGT AAAAGCTGAA 
AAGGTTGCTC CAACCAAATT GGAGAAGAAA CAAAAAGCTG AAACCAGGGC CCCAGTCAAA 
ACTGAGAAGA AGGACAAAGC AGAAAAGGAA ATCATACCAC AAGAAGACAA AAAAGTCAAG 
GTTTCTCAGG TGGAAGAAAA GACTAAGGAT GTCAAAGCTA AAACATCAGA GAGAAAAGTA 
GCAAAAGACT CAAAGAAAGA CGCTAAAGAG GAAGAAGAAA TCAAGACAAA GCCACATACT 
AAAGTGGAAA CACTGGATGC AGCAGAAACA ATGGGGGAAA TAAAAGATCA GTATAATTTC 
TGCCGTTATG TGGTTGACCT GTATTCTCAT GGAGATGCTT CTACAGAAGA AAAATCAAAG 
GAAAAACCAC AAGAGAAGAA AGAAGTTCTC CCACAAGAAA AACCAGGAGA ATTGAAAATA 
TCATTACCAG AAAAGAAAGA TATTCCTGAA ACAAAGGAAA CAGTCTTAGA GGAGGTGACA 
ATTCATGAAG CTGAAGACGA ATGCCCAAAG GAAGATTATG AACAAGTTAC ACCTCAAGCT 
ACTGAAAAAG AACCCCCAAA AGTCATGCAA GATCATTTAA CAGCTCATAA AGATACAGAA 
GAAAGTCCAA AAAAAGAGCA AGAACCCATG ACACCTCAGA TAGTAAAAGA TGGACCACCA 
AAACCAGTTG AAGCTGAAGT AAAAACAGAA GAGAAGAAAG AAGGAAGAAG CCAACAGCCT 
GCATTTACCG GTGTATCTAC AGATTTTGGC ATCATGCCTC GATTGATCAT AATCATGTCA 
GATTCACATG GTAGGTTTGC TTCAAGACCA GATGTCTCTG TAGATTGGAC CAAAAGAGAA 
ATGTTACTTA AAAAAGAGGA AAAAGAAGCT GAACCAATAA CACAAGAAAA GATGGACTAT 
ATAAAAGAAG CCCCAACTAC ATATATAATA GCAGAGGCTC CTGTGCAGAT TAATGTCACA 
AAACCACCAG ATGCAAAGAA AGAAATACAA ATGGAAATGC CTGTACGAAA ACTTGAAGAA 
GGACCAAAAG AAATAAAAAC AGAAATACCA GTGGAGATAC CTGAAGAAAA AACAAAAGAT 
GTAAAAGAAG TTGTAGTAAT ACCAGTGGAA ATGCCAGTGA AGAAAATTAA ACCGGAAGAA 
GAGATACCAG TGGAAATGCC AGTGAAAAAA CTTAAAGAAC CAGAAGAAAT ACCAGTGGAA 
ATACCAGTAG AGAAACCCAA AGAAGTATCA AAAGCAAAAG AACAAGAGGT GCCACTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCATTCAGAA GACTGAACAA 
GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAAAAAGAG 
GAACCAGTTA AGAAGGCTGA ACCAGAAGTA AAGGACATTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA 
AAACCTGCAG AAGTAAAGAA AGAGGTGCCC ATTAAGAAGA CTGAACCAGA AGTGAAGGAA 
GTTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCTGT AAAGAAACCT GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGTCC GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT 
GAACTGAAGA CACCAAAAGC CATTTATGAA GAAAAAACAA CCAAATCGAA TGATACAGCA 
AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902092.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757952.1 Xenopus tropicalis triadin (trdn), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OXa58389
Clone ID Related Accession (Same CDS sequence)	XM_031902092.1
Accession Version	XM_031902092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCCTCACTAA AAGCAGAGAA GAAGGAGAAA 
CCTGAAAAAA TTACCTTGAC AAAATCTGAG AAGAAAGAAA AATCTGCAAT AAAACCAGAG 
AAAAAAGACA AGACGGAAAA GGAAGCCTCA GTCAAAACTG AAAAGAAAGT AAAAGCTGCA 
AAATTAGACA AAGTCAAAAT TGACAAGAAA GAAAAAGCTG AAAAACTTGT CACACCCAAG 
TCTGAGAAGA AGGAAAAAAC TGAAAAAGTA ACCCCATCCA AACTTGAGAA AAAAGTAAAA 
GCTGAACAGA TAGCTCCCAC AAAACTAGAG AAAAAGGAAA AAACTGAAAA GGTAGCAACC 
ACAAAATCAG AAAAGAAAGA AAAAGCTGAA AAGTTAACCC CAGTCAAAGT AGAGAAGACA 
TTAAAACTTG AAAAGGTAGC ACCAGTTAAA GCTGAGAAAA AGGAAAAAGC TGAAAAAGTA 
GTCCCTGTTA AAGTTCAGAA AAAGGAAAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGAAA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGACA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTTCCAGT TAAAGCAGAG 
AAGAAAGGCA AAGCTGAAAA AGTAGCCCCT GTCAAACCCC AGAAGGAGAA AGCTGAAAAA 
GTAACCTCGG TCAAAGCTGA AAAGAAGGAA AAAGCTGAAA AAATTGCCCC AGTAAAAGCC 
GAGAAGAAGG AAAAAGTTGA AAAGATAGCC ACAAGTAAAG CCGAGAAGAA GGTTGAAAAG 
TTAGCCCCAG TCAAACCTGA GAAAAAGGAA AAGGCTAAAA AAGTAGCCCC AGTCAAATCT 
GAGAAGAAGG AAAAGACTGA AAAGTTAACC CAAGACACAA CCGAGAAGGT AAAAGCTGAA 
AAGGTTGCTC CAACCAAATT GGAGAAGAAA CAAAAAGCTG AAACCAGGGC CCCAGTCAAA 
ACTGAGAAGA AGGACAAAGC AGAAAAGGAA ATCATACCAC AAGAAGACAA AAAAGTCAAG 
GTTTCTCAGG TGGAAGAAAA GACTAAGGAT GTCAAAGCTA AAACATCAGA GAGAAAAGTA 
GCAAAAGACT CAAAGAAAGA CGCTAAAGAG GAAGAAGAAA TCAAGACAAA GCCACATACT 
AAAGTGGAAA CACTGGATGC AGCAGAAACA ATGGGGGAAA TAAAAGATCA GTATAATTTC 
TGCCGTTATG TGGTTGACCT GTATTCTCAT GGAGATGCTT CTACAGAAGA AAAATCAAAG 
GAAAAACCAC AAGAGAAGAA AGAAGTTCTC CCACAAGAAA AACCAGGAGA ATTGAAAATA 
TCATTACCAG AAAAGAAAGA TATTCCTGAA ACAAAGGAAA CAGTCTTAGA GGAGGTGACA 
ATTCATGAAG CTGAAGACGA ATGCCCAAAG GAAGATTATG AACAAGTTAC ACCTCAAGCT 
ACTGAAAAAG AACCCCCAAA AGTCATGCAA GATCATTTAA CAGCTCATAA AGATACAGAA 
GAAAGTCCAA AAAAAGAGCA AGAACCCATG ACACCTCAGA TAGTAAAAGA TGGACCACCA 
AAACCAGTTG AAGCTGAAGT AAAAACAGAA GAGAAGAAAG AAGGAAGAAG CCAACAGCCT 
GCATTTACCG GTGTATCTAC AGATTTTGGC ATCATGCCTC GATTGATCAT AATCATGTCA 
GATTCACATG GTAGGTTTGC TTCAAGACCA GATGTCTCTG TAGATTGGAC CAAAAGAGAA 
ATGTTACTTA AAAAAGAGGA AAAAGAAGCT GAACCAATAA CACAAGAAAA GATGGACTAT 
ATAAAAGAAG CCCCAACTAC ATATATAATA GCAGAGGCTC CTGTGCAGAT TAATGTCACA 
AAACCACCAG ATGCAAAGAA AGAAATACAA ATGGAAATGC CTGTACGAAA ACTTGAAGAA 
GGACCAAAAG AAATAAAAAC AGAAATACCA GTGGAGATAC CTGAAGAAAA AACAAAAGAT 
GTAAAAGAAG TTGTAGTAAT ACCAGTGGAA ATGCCAGTGA AGAAAATTAA ACCGGAAGAA 
GAGATACCAG TGGAAATGCC AGTGAAAAAA CTTAAAGAAC CAGAAGAAAT ACCAGTGGAA 
ATACCAGTAG AGAAACCCAA AGAAGTATCA AAAGCAAAAG AACAAGAGGT GCCACTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCATTCAGAA GACTGAACAA 
GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAAAAAGAG 
GAACCAGTTA AGAAGGCTGA ACCAGAAGTA AAGGACATTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA 
AAACCTGCAG AAGTAAAGAA AGAGGTGCCC ATTAAGAAGA CTGAACCAGA AGTGAAGGAA 
GTTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCTGT AAAGAAACCT GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGTCC GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT 
GAACTGAAGA CACCAAAAGC CATTTATGAA GAAAAAACAA CCAAATCGAA TGATACAGCA 
AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757952.1
CDS	383..4657
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902092.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902092.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCCTCACTAA AAGCAGAGAA GAAGGAGAAA 
CCTGAAAAAA TTACCTTGAC AAAATCTGAG AAGAAAGAAA AATCTGCAAT AAAACCAGAG 
AAAAAAGACA AGACGGAAAA GGAAGCCTCA GTCAAAACTG AAAAGAAAGT AAAAGCTGCA 
AAATTAGACA AAGTCAAAAT TGACAAGAAA GAAAAAGCTG AAAAACTTGT CACACCCAAG 
TCTGAGAAGA AGGAAAAAAC TGAAAAAGTA ACCCCATCCA AACTTGAGAA AAAAGTAAAA 
GCTGAACAGA TAGCTCCCAC AAAACTAGAG AAAAAGGAAA AAACTGAAAA GGTAGCAACC 
ACAAAATCAG AAAAGAAAGA AAAAGCTGAA AAGTTAACCC CAGTCAAAGT AGAGAAGACA 
TTAAAACTTG AAAAGGTAGC ACCAGTTAAA GCTGAGAAAA AGGAAAAAGC TGAAAAAGTA 
GTCCCTGTTA AAGTTCAGAA AAAGGAAAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGAAA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGACA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTTCCAGT TAAAGCAGAG 
AAGAAAGGCA AAGCTGAAAA AGTAGCCCCT GTCAAACCCC AGAAGGAGAA AGCTGAAAAA 
GTAACCTCGG TCAAAGCTGA AAAGAAGGAA AAAGCTGAAA AAATTGCCCC AGTAAAAGCC 
GAGAAGAAGG AAAAAGTTGA AAAGATAGCC ACAAGTAAAG CCGAGAAGAA GGTTGAAAAG 
TTAGCCCCAG TCAAACCTGA GAAAAAGGAA AAGGCTAAAA AAGTAGCCCC AGTCAAATCT 
GAGAAGAAGG AAAAGACTGA AAAGTTAACC CAAGACACAA CCGAGAAGGT AAAAGCTGAA 
AAGGTTGCTC CAACCAAATT GGAGAAGAAA CAAAAAGCTG AAACCAGGGC CCCAGTCAAA 
ACTGAGAAGA AGGACAAAGC AGAAAAGGAA ATCATACCAC AAGAAGACAA AAAAGTCAAG 
GTTTCTCAGG TGGAAGAAAA GACTAAGGAT GTCAAAGCTA AAACATCAGA GAGAAAAGTA 
GCAAAAGACT CAAAGAAAGA CGCTAAAGAG GAAGAAGAAA TCAAGACAAA GCCACATACT 
AAAGTGGAAA CACTGGATGC AGCAGAAACA ATGGGGGAAA TAAAAGATCA GTATAATTTC 
TGCCGTTATG TGGTTGACCT GTATTCTCAT GGAGATGCTT CTACAGAAGA AAAATCAAAG 
GAAAAACCAC AAGAGAAGAA AGAAGTTCTC CCACAAGAAA AACCAGGAGA ATTGAAAATA 
TCATTACCAG AAAAGAAAGA TATTCCTGAA ACAAAGGAAA CAGTCTTAGA GGAGGTGACA 
ATTCATGAAG CTGAAGACGA ATGCCCAAAG GAAGATTATG AACAAGTTAC ACCTCAAGCT 
ACTGAAAAAG AACCCCCAAA AGTCATGCAA GATCATTTAA CAGCTCATAA AGATACAGAA 
GAAAGTCCAA AAAAAGAGCA AGAACCCATG ACACCTCAGA TAGTAAAAGA TGGACCACCA 
AAACCAGTTG AAGCTGAAGT AAAAACAGAA GAGAAGAAAG AAGGAAGAAG CCAACAGCCT 
GCATTTACCG GTGTATCTAC AGATTTTGGC ATCATGCCTC GATTGATCAT AATCATGTCA 
GATTCACATG GTAGGTTTGC TTCAAGACCA GATGTCTCTG TAGATTGGAC CAAAAGAGAA 
ATGTTACTTA AAAAAGAGGA AAAAGAAGCT GAACCAATAA CACAAGAAAA GATGGACTAT 
ATAAAAGAAG CCCCAACTAC ATATATAATA GCAGAGGCTC CTGTGCAGAT TAATGTCACA 
AAACCACCAG ATGCAAAGAA AGAAATACAA ATGGAAATGC CTGTACGAAA ACTTGAAGAA 
GGACCAAAAG AAATAAAAAC AGAAATACCA GTGGAGATAC CTGAAGAAAA AACAAAAGAT 
GTAAAAGAAG TTGTAGTAAT ACCAGTGGAA ATGCCAGTGA AGAAAATTAA ACCGGAAGAA 
GAGATACCAG TGGAAATGCC AGTGAAAAAA CTTAAAGAAC CAGAAGAAAT ACCAGTGGAA 
ATACCAGTAG AGAAACCCAA AGAAGTATCA AAAGCAAAAG AACAAGAGGT GCCACTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCATTCAGAA GACTGAACAA 
GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAAAAAGAG 
GAACCAGTTA AGAAGGCTGA ACCAGAAGTA AAGGACATTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA 
AAACCTGCAG AAGTAAAGAA AGAGGTGCCC ATTAAGAAGA CTGAACCAGA AGTGAAGGAA 
GTTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCTGT AAAGAAACCT GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGTCC GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT 
GAACTGAAGA CACCAAAAGC CATTTATGAA GAAAAAACAA CCAAATCGAA TGATACAGCA 
AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902093.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757953.1 Xenopus tropicalis triadin (trdn), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OXa58390
Clone ID Related Accession (Same CDS sequence)	XM_031902093.1
Accession Version	XM_031902093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GTCCGAGCCA AAACCTGCAG AAGTTAAGAA AGATGAACTG 
AAGACACCAA AAGCCATTTA TGAAGAAAAA ACAACCAAAT CGAATGATAC AGCAAATATG 
CTGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757953.1
CDS	383..4651
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902093.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902093.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GTCCGAGCCA AAACCTGCAG AAGTTAAGAA AGATGAACTG 
AAGACACCAA AAGCCATTTA TGAAGAAAAA ACAACCAAAT CGAATGATAC AGCAAATATG 
CTGTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902094.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757954.1 Xenopus tropicalis triadin (trdn), transcript variant X28, mRNA.

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CloneID	OXa58391
Clone ID Related Accession (Same CDS sequence)	XM_031902094.1
Accession Version	XM_031902094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGG AAGTTCACTT 
AACAAGTTCC GGAGTAATCC CGTCGGAATA ATACATGAAG CAGTGGATGA AACAACTGAT 
TGGATTTATG GAATATATAT TACTATATCT GACTTGATAT CCCTTGATGA AGAACGTAAA 
GGGCATGCTG AGCCTCCTGT AAAGAAAATA GGTGGAATAC AACCAGCTCT ACACAGAAAA 
GTAATTCATA AAGAGAAACC TGAAGTAAAA GAGAACATTT TGAAAAAGGC CTCAGCTAAA 
GCAATACCAA AACTCAAGGC TGAAGAGGAA GATGAGCCAG TTAAGAAAAC CAGAGCAAAA 
GAAAAGGCTG AAACAAAAGA AAAAGTAGAG AAGAAAATTC TACCTAAAGC CTCACTAAAA 
GCAGAGAAGA AGGAGAAACC TGAAAAAATT ACCTTGACAA AATCTGAGAA GAAAGAAAAA 
TCTGCAATAA AACCAGAGAA AAAAGACAAG ACGGAAAAGG AAGCCTCAGT CAAAACTGAA 
AAGAAAGTAA AAGCTGCAAA ATTAGACAAA GTCAAAATTG ACAAGAAAGA AAAAGCTGAA 
AAACTTGTCA CACCCAAGTC TGAGAAGAAG GAAAAAACTG AAAAAGTAAC CCCATCCAAA 
CTTGAGAAAA AAGTAAAAGC TGAACAGATA GCTCCCACAA AACTAGAGAA AAAGGAAAAA 
ACTGAAAAGG TAGCAACCAC AAAATCAGAA AAGAAAGAAA AAGCTGAAAA GTTAACCCCA 
GTCAAAGTAG AGAAGACATT AAAACTTGAA AAGGTAGCAC CAGTTAAAGC TGAGAAAAAG 
GAAAAAGCTG AAAAAGTAGT CCCTGTTAAA GTTCAGAAAA AGGAAAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGAAAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGACAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTTCCAGTTA AAGCAGAGAA GAAAGGCAAA GCTGAAAAAG TAGCCCCTGT CAAACCCCAG 
AAGGAGAAAG CTGAAAAAGT AACCTCGGTC AAAGCTGAAA AGAAGGAAAA AGCTGAAAAA 
ATTGCCCCAG TAAAAGCCGA GAAGAAGGAA AAAGTTGAAA AGATAGCCAC AAGTAAAGCC 
GAGAAGAAGG TTGAAAAGTT AGCCCCAGTC AAACCTGAGA AAAAGGAAAA GGCTAAAAAA 
GTAGCCCCAG TCAAATCTGA GAAGAAGGAA AAGACTGAAA AGTTAACCCA AGACACAACC 
GAGAAGGTAA AAGCTGAAAA GGTTGCTCCA ACCAAATTGG AGAAGAAACA AAAAGCTGAA 
ACCAGGGCCC CAGTCAAAAC TGAGAAGAAG GACAAAGCAG AAAAGGAAAT CATACCACAA 
GAAGACAAAA AAGTCAAGGT TTCTCAGGTG GAAGAAAAGA CTAAGGATGT CAAAGCTAAA 
ACATCAGAGA GAAAAGTAGC AAAAGACTCA AAGAAAGACG CTAAAGAGGA AGAAGAAATC 
AAGACAAAGC CACATACTAA AGTGGAAACA CTGGATGCAG CAGAAACAAT GGGGGAAATA 
AAAGATCAGT ATAATTTCTG CCGTTATGTG GTTGACCTGT ATTCTCATGG AGATGCTTCT 
ACAGAAGAAA AATCAAAGGA AAAACCACAA GAGAAGAAAG AAGTTCTCCC ACAAGAAAAA 
CCAGGAGAAT TGAAAATATC ATTACCAGAA AAGAAAGATA TTCCTGAAAC AAAGGAAACA 
GTCTTAGAGG AGGTGACAAT TCATGAAGCT GAAGACGAAT GCCCAAAGGA AGATTATGAA 
CAAGTTACAC CTCAAGCTAC TGAAAAAGAA CCCCCAAAAG TCATGCAAGA TCATTTAACA 
GCTCATAAAG ATACAGAAGA AAGTCCAAAA AAAGAGCAAG AACCCATGAC ACCTCAGATA 
GTAAAAGATG GACCACCAAA ACCAGTTGAA GCTGAAGTAA AAACAGAAGA GAAGAAAGAA 
GGAAGAAGCC AACAGCCTGC ATTTACCGGT GTATCTACAG ATTTTGGCAT CATGCCTCGA 
TTGATCATAA TCATGTCAGA TTCACATGGT AGGTTTGCTT CAAGACCAGA TGTCTCTGTA 
GATTGGACCA AAAGAGAAAT GTTACTTAAA AAAGAGGAAA AAGAAGCTGA ACCAATAACA 
CAAGAAAAGA TGGACTATAT AAAAGAAGCC CCAACTACAT ATATAATAGC AGAGGCTCCT 
GTGCAGATTA ATGTCACAAA ACCACCAGAT GCAAAGAAAG AAATACAAAT GGAAATGCCT 
GTACGAAAAC TTGAAGAAGG ACCAAAAGAA ATAAAAACAG AAATACCAGT GGAGATACCT 
GAAGAAAAAA CAAAAGATGT AAAAGAAGTT GTAGTAATAC CAGTGGAAAT GCCAGTGAAG 
AAAATTAAAC CGGAAGAAGA GATACCAGTG GAAATGCCAG TGAAAAAACT TAAAGAACCA 
GAAGAAATAC CAGTGGAAAT ACCAGTAGAG AAACCCAAAG AAGTATCAAA AGCAAAAGAA 
CAAGAGGTGC CACTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTCAGAAGA CTGAACAAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757954.1
CDS	383..4615
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902094.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902094.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGG AAGTTCACTT 
AACAAGTTCC GGAGTAATCC CGTCGGAATA ATACATGAAG CAGTGGATGA AACAACTGAT 
TGGATTTATG GAATATATAT TACTATATCT GACTTGATAT CCCTTGATGA AGAACGTAAA 
GGGCATGCTG AGCCTCCTGT AAAGAAAATA GGTGGAATAC AACCAGCTCT ACACAGAAAA 
GTAATTCATA AAGAGAAACC TGAAGTAAAA GAGAACATTT TGAAAAAGGC CTCAGCTAAA 
GCAATACCAA AACTCAAGGC TGAAGAGGAA GATGAGCCAG TTAAGAAAAC CAGAGCAAAA 
GAAAAGGCTG AAACAAAAGA AAAAGTAGAG AAGAAAATTC TACCTAAAGC CTCACTAAAA 
GCAGAGAAGA AGGAGAAACC TGAAAAAATT ACCTTGACAA AATCTGAGAA GAAAGAAAAA 
TCTGCAATAA AACCAGAGAA AAAAGACAAG ACGGAAAAGG AAGCCTCAGT CAAAACTGAA 
AAGAAAGTAA AAGCTGCAAA ATTAGACAAA GTCAAAATTG ACAAGAAAGA AAAAGCTGAA 
AAACTTGTCA CACCCAAGTC TGAGAAGAAG GAAAAAACTG AAAAAGTAAC CCCATCCAAA 
CTTGAGAAAA AAGTAAAAGC TGAACAGATA GCTCCCACAA AACTAGAGAA AAAGGAAAAA 
ACTGAAAAGG TAGCAACCAC AAAATCAGAA AAGAAAGAAA AAGCTGAAAA GTTAACCCCA 
GTCAAAGTAG AGAAGACATT AAAACTTGAA AAGGTAGCAC CAGTTAAAGC TGAGAAAAAG 
GAAAAAGCTG AAAAAGTAGT CCCTGTTAAA GTTCAGAAAA AGGAAAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGAAAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGACAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTTCCAGTTA AAGCAGAGAA GAAAGGCAAA GCTGAAAAAG TAGCCCCTGT CAAACCCCAG 
AAGGAGAAAG CTGAAAAAGT AACCTCGGTC AAAGCTGAAA AGAAGGAAAA AGCTGAAAAA 
ATTGCCCCAG TAAAAGCCGA GAAGAAGGAA AAAGTTGAAA AGATAGCCAC AAGTAAAGCC 
GAGAAGAAGG TTGAAAAGTT AGCCCCAGTC AAACCTGAGA AAAAGGAAAA GGCTAAAAAA 
GTAGCCCCAG TCAAATCTGA GAAGAAGGAA AAGACTGAAA AGTTAACCCA AGACACAACC 
GAGAAGGTAA AAGCTGAAAA GGTTGCTCCA ACCAAATTGG AGAAGAAACA AAAAGCTGAA 
ACCAGGGCCC CAGTCAAAAC TGAGAAGAAG GACAAAGCAG AAAAGGAAAT CATACCACAA 
GAAGACAAAA AAGTCAAGGT TTCTCAGGTG GAAGAAAAGA CTAAGGATGT CAAAGCTAAA 
ACATCAGAGA GAAAAGTAGC AAAAGACTCA AAGAAAGACG CTAAAGAGGA AGAAGAAATC 
AAGACAAAGC CACATACTAA AGTGGAAACA CTGGATGCAG CAGAAACAAT GGGGGAAATA 
AAAGATCAGT ATAATTTCTG CCGTTATGTG GTTGACCTGT ATTCTCATGG AGATGCTTCT 
ACAGAAGAAA AATCAAAGGA AAAACCACAA GAGAAGAAAG AAGTTCTCCC ACAAGAAAAA 
CCAGGAGAAT TGAAAATATC ATTACCAGAA AAGAAAGATA TTCCTGAAAC AAAGGAAACA 
GTCTTAGAGG AGGTGACAAT TCATGAAGCT GAAGACGAAT GCCCAAAGGA AGATTATGAA 
CAAGTTACAC CTCAAGCTAC TGAAAAAGAA CCCCCAAAAG TCATGCAAGA TCATTTAACA 
GCTCATAAAG ATACAGAAGA AAGTCCAAAA AAAGAGCAAG AACCCATGAC ACCTCAGATA 
GTAAAAGATG GACCACCAAA ACCAGTTGAA GCTGAAGTAA AAACAGAAGA GAAGAAAGAA 
GGAAGAAGCC AACAGCCTGC ATTTACCGGT GTATCTACAG ATTTTGGCAT CATGCCTCGA 
TTGATCATAA TCATGTCAGA TTCACATGGT AGGTTTGCTT CAAGACCAGA TGTCTCTGTA 
GATTGGACCA AAAGAGAAAT GTTACTTAAA AAAGAGGAAA AAGAAGCTGA ACCAATAACA 
CAAGAAAAGA TGGACTATAT AAAAGAAGCC CCAACTACAT ATATAATAGC AGAGGCTCCT 
GTGCAGATTA ATGTCACAAA ACCACCAGAT GCAAAGAAAG AAATACAAAT GGAAATGCCT 
GTACGAAAAC TTGAAGAAGG ACCAAAAGAA ATAAAAACAG AAATACCAGT GGAGATACCT 
GAAGAAAAAA CAAAAGATGT AAAAGAAGTT GTAGTAATAC CAGTGGAAAT GCCAGTGAAG 
AAAATTAAAC CGGAAGAAGA GATACCAGTG GAAATGCCAG TGAAAAAACT TAAAGAACCA 
GAAGAAATAC CAGTGGAAAT ACCAGTAGAG AAACCCAAAG AAGTATCAAA AGCAAAAGAA 
CAAGAGGTGC CACTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTCAGAAGA CTGAACAAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902095.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757955.1 Xenopus tropicalis triadin (trdn), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OXa58392
Clone ID Related Accession (Same CDS sequence)	XM_031902095.1
Accession Version	XM_031902095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757955.1
CDS	383..4615
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902095.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902095.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902096.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757956.1 Xenopus tropicalis triadin (trdn), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OXa58393
Clone ID Related Accession (Same CDS sequence)	XM_031902096.1
Accession Version	XM_031902096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757956.1
CDS	383..4615
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902096.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902096.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902098.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757958.1 Xenopus tropicalis triadin (trdn), transcript variant X32, mRNA.

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CloneID	OXa58394
Clone ID Related Accession (Same CDS sequence)	XM_031902098.1 , XM_031902097.1
Accession Version	XM_031902098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757958.1
CDS	383..4615
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902098.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902098.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902097.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757957.1 Xenopus tropicalis triadin (trdn), transcript variant X31, mRNA.

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CloneID	OXa58394
Clone ID Related Accession (Same CDS sequence)	XM_031902098.1 , XM_031902097.1
Accession Version	XM_031902097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757957.1
CDS	383..4615
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902097.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902097.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902099.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757959.1 Xenopus tropicalis triadin (trdn), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OXa58395
Clone ID Related Accession (Same CDS sequence)	XM_031902099.1
Accession Version	XM_031902099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757959.1
CDS	384..4616
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902099.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902099.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902100.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757960.1 Xenopus tropicalis triadin (trdn), transcript variant X34, mRNA.

Price & Availability↑

CloneID	OXa58396
Clone ID Related Accession (Same CDS sequence)	XM_031902100.1
Accession Version	XM_031902100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757960.1
CDS	384..4616
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902100.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902100.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902101.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757961.1 Xenopus tropicalis triadin (trdn), transcript variant X35, mRNA.

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CloneID	OXa58397
Clone ID Related Accession (Same CDS sequence)	XM_031902101.1
Accession Version	XM_031902101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757961.1
CDS	384..4616
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902101.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902101.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902102.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757962.1 Xenopus tropicalis triadin (trdn), transcript variant X36, mRNA.

Price & Availability↑

CloneID	OXa58398
Clone ID Related Accession (Same CDS sequence)	XM_031902102.1
Accession Version	XM_031902102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757962.1
CDS	384..4616
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902102.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902102.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902103.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757963.1 Xenopus tropicalis triadin (trdn), transcript variant X37, mRNA.

Price & Availability↑

CloneID	OXa58399
Clone ID Related Accession (Same CDS sequence)	XM_031902103.1
Accession Version	XM_031902103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757963.1
CDS	384..4616
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902103.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902103.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902104.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757964.1 Xenopus tropicalis triadin (trdn), transcript variant X38, mRNA.

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CloneID	OXa58400
Clone ID Related Accession (Same CDS sequence)	XM_031902104.1
Accession Version	XM_031902104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X38
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757964.1
CDS	384..4616
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902104.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902104.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902105.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757965.1 Xenopus tropicalis triadin (trdn), transcript variant X39, mRNA.

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CloneID	OXa58401
Clone ID Related Accession (Same CDS sequence)	XM_031902105.1
Accession Version	XM_031902105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X39
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757965.1
CDS	384..4616
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902105.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902105.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902106.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757966.1 Xenopus tropicalis triadin (trdn), transcript variant X40, mRNA.

Price & Availability↑

CloneID	OXa58402
Clone ID Related Accession (Same CDS sequence)	XM_031902106.1
Accession Version	XM_031902106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757966.1
CDS	384..4616
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902106.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902106.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902107.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757967.1 Xenopus tropicalis triadin (trdn), transcript variant X41, mRNA.

Price & Availability↑

CloneID	OXa58403
Clone ID Related Accession (Same CDS sequence)	XM_031902107.1
Accession Version	XM_031902107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X41
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757967.1
CDS	384..4616
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902107.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902107.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902108.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757968.1 Xenopus tropicalis triadin (trdn), transcript variant X42, mRNA.

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CloneID	OXa58404
Clone ID Related Accession (Same CDS sequence)	XM_031902108.1
Accession Version	XM_031902108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X42
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGATCATT TAACAGCTCA TAAAGATACA 
GAAGAAAGTC CAAAAAAAGA GCAAGCTGAA GTAAAAACAG AAGAGAAGAA AGAAGGAAGA 
AGCCAACAGC CTGCATTTAC CGGTGTATCT ACAGATTTTG GCATCATGCC TCGATTGATC 
ATAATCATGT CAGATTCACA TGGTAGGTTT GCTTCAAGAC CAGATGTCTC TGTAGATTGG 
ACCAAAAGAG AAATGTTACT TAAAAAAGAG GAAAAAGAAG CTGAACCAAT AACACAAGAA 
AAGATGGACT ATATAAAAGA AGCCCCAACT ACATATATAA TAGCAGAGGC TCCTGTGCAG 
ATTAATGTCA CAAAACCACC AGATGCAAAG AAAGAAATAC AAATGGAAAT GCCTGTACGA 
AAACTTGAAG AAGGACCAAA AGAAATAAAA ACAGAAATAC CAGTGGAGAT ACCTGAAGAA 
AAAACAAAAG ATGTAAAAGA AGTTGTAGTA ATACCAGTGG AAATGCCAGT GAAGAAAATT 
AAACCGGAAG AAGAGATACC AGTGGAAATG CCAGTGAAAA AACTTAAAGA ACCAGAAGAA 
ATACCAGTGG AAATACCAGT AGAGAAACCC AAAGAAGTAT CAAAAGCAAA AGAACAAGAG 
GTGCCACTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCATTCAG 
AAGACTGAAC AAGAAGTGAA GGAAGTTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCC TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAAAAAG AGGAACCAGT TAAGAAGGCT GAACCAGAAG TAAAGGACAT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG 
AAACCTGAAG CAAAACCTGC AGAAGTAAAG AAAGAGGTGC CCATTAAGAA GACTGAACCA 
GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAAAAAGAG 
GAACCAGTTA AGAAGGCTGA ACCAGAAGTA AAGGACATTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA 
AAACCTGCAG AAGTAAAGAA AGAGGTGCCC ATTAAGAAGA CTGAACCAGA AGTGAAGGAA 
GTTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGT CCGAGCCAAA ACCTGCAGAA 
GTTAAGAAAG ATGAACTGAA GACACCAAAA GCCATTTATG AAGAAAAAAC AACCAAATCG 
AATGATACAG CAAATATGCT GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757968.1
CDS	383..4609
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYDHLTAHKDTEESPKKEQAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902108.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X42, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902108.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGATCATT TAACAGCTCA TAAAGATACA 
GAAGAAAGTC CAAAAAAAGA GCAAGCTGAA GTAAAAACAG AAGAGAAGAA AGAAGGAAGA 
AGCCAACAGC CTGCATTTAC CGGTGTATCT ACAGATTTTG GCATCATGCC TCGATTGATC 
ATAATCATGT CAGATTCACA TGGTAGGTTT GCTTCAAGAC CAGATGTCTC TGTAGATTGG 
ACCAAAAGAG AAATGTTACT TAAAAAAGAG GAAAAAGAAG CTGAACCAAT AACACAAGAA 
AAGATGGACT ATATAAAAGA AGCCCCAACT ACATATATAA TAGCAGAGGC TCCTGTGCAG 
ATTAATGTCA CAAAACCACC AGATGCAAAG AAAGAAATAC AAATGGAAAT GCCTGTACGA 
AAACTTGAAG AAGGACCAAA AGAAATAAAA ACAGAAATAC CAGTGGAGAT ACCTGAAGAA 
AAAACAAAAG ATGTAAAAGA AGTTGTAGTA ATACCAGTGG AAATGCCAGT GAAGAAAATT 
AAACCGGAAG AAGAGATACC AGTGGAAATG CCAGTGAAAA AACTTAAAGA ACCAGAAGAA 
ATACCAGTGG AAATACCAGT AGAGAAACCC AAAGAAGTAT CAAAAGCAAA AGAACAAGAG 
GTGCCACTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCATTCAG 
AAGACTGAAC AAGAAGTGAA GGAAGTTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCC TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAAAAAG AGGAACCAGT TAAGAAGGCT GAACCAGAAG TAAAGGACAT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG 
AAACCTGAAG CAAAACCTGC AGAAGTAAAG AAAGAGGTGC CCATTAAGAA GACTGAACCA 
GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAAAAAGAG 
GAACCAGTTA AGAAGGCTGA ACCAGAAGTA AAGGACATTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA 
AAACCTGCAG AAGTAAAGAA AGAGGTGCCC ATTAAGAAGA CTGAACCAGA AGTGAAGGAA 
GTTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGT CCGAGCCAAA ACCTGCAGAA 
GTTAAGAAAG ATGAACTGAA GACACCAAAA GCCATTTATG AAGAAAAAAC AACCAAATCG 
AATGATACAG CAAATATGCT GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902109.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757969.1 Xenopus tropicalis triadin (trdn), transcript variant X43, mRNA.

Price & Availability↑

CloneID	OXa58405
Clone ID Related Accession (Same CDS sequence)	XM_031902109.1
Accession Version	XM_031902109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X43
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCCTCACTAA AAGCAGAGAA GAAGGAGAAA 
CCTGAAAAAA TTACCTTGAC AAAATCTGAG AAGAAAGAAA AATCTGCAAT AAAACCAGAG 
AAAAAAGACA AGACGGAAAA GGAAGCCTCA GTCAAAACTG AAAAGAAAGT AAAAGCTGCA 
AAATTAGACA AAGTCAAAAT TGACAAGAAA GAAAAAGCTG AAAAACTTGT CACACCCAAG 
TCTGAGAAGA AGGAAAAAAC TGAAAAAGTA ACCCCATCCA AACTTGAGAA AAAAGTAAAA 
GCTGAACAGA TAGCTCCCAC AAAACTAGAG AAAAAGGAAA AAACTGAAAA GGTAGCAACC 
ACAAAATCAG AAAAGAAAGA AAAAGCTGAA AAGTTAACCC CAGTCAAAGT AGAGAAGACA 
TTAAAACTTG AAAAGGTAGC ACCAGTTAAA GCTGAGAAAA AGGAAAAAGC TGAAAAAGTA 
GTCCCTGTTA AAGTTCAGAA AAAGGAAAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGAAA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGACA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTTCCAGT TAAAGCAGAG 
AAGAAAGGCA AAGCTGAAAA AGTAGCCCCT GTCAAACCCC AGAAGGAGAA AGCTGAAAAA 
GTAACCTCGG TCAAAGCTGA AAAGAAGGAA AAAGCTGAAA AAATTGCCCC AGTAAAAGCC 
GAGAAGAAGG AAAAAGTTGA AAAGATAGCC ACAAGTAAAG CCGAGAAGAA GGTTGAAAAG 
TTAGCCCCAG TCAAACCTGA GAAAAAGGAA AAGGCTAAAA AAGTAGCCCC AGTCAAATCT 
GAGAAGAAGG AAAAGACTGA AAAGTTAACC CAAGACACAA CCGAGAAGGT AAAAGCTGAA 
AAGGTTGCTC CAACCAAATT GGAGAAGAAA CAAAAAGCTG AAACCAGGGC CCCAGTCAAA 
ACTGAGAAGA AGGACAAAGC AGAAAAGGAA ATCATACCAC AAGAAGACAA AAAAGTCAAG 
GTTTCTCAGG TGGAAGAAAA GACTAAGGAT GTCAAAGCTA AAACATCAGA GAGAAAAGTA 
GCAAAAGACT CAAAGAAAGA CGCTAAAGAG GAAGAAGAAA TCAAGACAAA GCCACATACT 
AAAGTGGAAA CACTGGATGC AGCAGAAACA ATGGGGGAAA TAAAAGATCA GTATAATTTC 
TGCCGTTATG TGGTTGACCT GTATTCTCAT GGAGATGCTT CTACAGAAGA AAAATCAAAG 
GAAAAACCAC AAGAGAAGAA AGAAGTTCTC CCACAAGAAA AACCAGGAGA ATTGAAAATA 
TCATTACCAG AAAAGAAAGA TATTCCTGAA ACAAAGGAAA CAGTCTTAGA GGAGGTGACA 
ATTCATGAAG CTGAAGACGA ATGCCCAAAG GAAGATTATG AACAAGTTAC ACCTCAAGCT 
ACTGAAAAAG AACCCCCAAA AGTCATGCAA GATCATTTAA CAGCTCATAA AGATACAGAA 
GAAAGTCCAA AAAAAGAGCA AGAACCCATG ACACCTCAGA TAGTAAAAGA TGGACCACCA 
AAACCAGTTG AAGCTGAAGT AAAAACAGAA GAGAAGAAAG AAGGAAGAAG CCAACAGCCT 
GCATTTACCG GTGTATCTAC AGATTTTGGC ATCATGCCTC GATTGATCAT AATCATGTCA 
GATTCACATG GTAGGTTTGC TTCAAGACCA GATGTCTCTG TAGATTGGAC CAAAAGAGAA 
ATGTTACTTA AAAAAGAGGA AAAAGAAGCT GAACCAATAA CACAAGAAAA GATGGACTAT 
ATAAAAGAAG CCCCAACTAC ATATATAATA GCAGAGGCTC CTGTGCAGAT TAATGTCACA 
AAACCACCAG ATGCAAAGAA AGAAATACAA ATGGAAATGC CTGTACGAAA ACTTGAAGAA 
GGACCAAAAG AAATAAAAAC AGAAATACCA GTGGAGATAC CTGAAGAAAA AACAAAAGAT 
GTAAAAGAAG TTGTAGTAAT ACCAGTGGAA ATGCCAGTGA AGAAAATTAA ACCGGAAGAA 
GAGATACCAG TGGAAATGCC AGTGAAAAAA CTTAAAGAAC CAGAAGAAAT ACCAGTGGAA 
ATACCAGTAG AGAAACCCAA AGAAGTATCA AAAGCAAAAG AACAAGAGGT GCCACTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCATTCAGAA GACTGAACAA 
GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAAAAAGAG 
GAACCAGTTA AGAAGGCTGA ACCAGAAGTA AAGGACATTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA 
AAACCTGCAG AAGTAAAGAA AGAGGTGCCC ATTAAGAAGA CTGAACCAGA AGTGAAGGAA 
GTTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCTGT AAAGAAACCT GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGTCC GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT 
GAACTGAAGA CACCAAAAGC CATTTATGAA GAAAAAACAA CCAAATCGAA TGATACAGCA 
AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757969.1
CDS	383..4597
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKAIPKLKAEEEDEPVKKTRASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902109.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X43, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902109.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCCTCACTAA AAGCAGAGAA GAAGGAGAAA 
CCTGAAAAAA TTACCTTGAC AAAATCTGAG AAGAAAGAAA AATCTGCAAT AAAACCAGAG 
AAAAAAGACA AGACGGAAAA GGAAGCCTCA GTCAAAACTG AAAAGAAAGT AAAAGCTGCA 
AAATTAGACA AAGTCAAAAT TGACAAGAAA GAAAAAGCTG AAAAACTTGT CACACCCAAG 
TCTGAGAAGA AGGAAAAAAC TGAAAAAGTA ACCCCATCCA AACTTGAGAA AAAAGTAAAA 
GCTGAACAGA TAGCTCCCAC AAAACTAGAG AAAAAGGAAA AAACTGAAAA GGTAGCAACC 
ACAAAATCAG AAAAGAAAGA AAAAGCTGAA AAGTTAACCC CAGTCAAAGT AGAGAAGACA 
TTAAAACTTG AAAAGGTAGC ACCAGTTAAA GCTGAGAAAA AGGAAAAAGC TGAAAAAGTA 
GTCCCTGTTA AAGTTCAGAA AAAGGAAAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGAAA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGACA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTTCCAGT TAAAGCAGAG 
AAGAAAGGCA AAGCTGAAAA AGTAGCCCCT GTCAAACCCC AGAAGGAGAA AGCTGAAAAA 
GTAACCTCGG TCAAAGCTGA AAAGAAGGAA AAAGCTGAAA AAATTGCCCC AGTAAAAGCC 
GAGAAGAAGG AAAAAGTTGA AAAGATAGCC ACAAGTAAAG CCGAGAAGAA GGTTGAAAAG 
TTAGCCCCAG TCAAACCTGA GAAAAAGGAA AAGGCTAAAA AAGTAGCCCC AGTCAAATCT 
GAGAAGAAGG AAAAGACTGA AAAGTTAACC CAAGACACAA CCGAGAAGGT AAAAGCTGAA 
AAGGTTGCTC CAACCAAATT GGAGAAGAAA CAAAAAGCTG AAACCAGGGC CCCAGTCAAA 
ACTGAGAAGA AGGACAAAGC AGAAAAGGAA ATCATACCAC AAGAAGACAA AAAAGTCAAG 
GTTTCTCAGG TGGAAGAAAA GACTAAGGAT GTCAAAGCTA AAACATCAGA GAGAAAAGTA 
GCAAAAGACT CAAAGAAAGA CGCTAAAGAG GAAGAAGAAA TCAAGACAAA GCCACATACT 
AAAGTGGAAA CACTGGATGC AGCAGAAACA ATGGGGGAAA TAAAAGATCA GTATAATTTC 
TGCCGTTATG TGGTTGACCT GTATTCTCAT GGAGATGCTT CTACAGAAGA AAAATCAAAG 
GAAAAACCAC AAGAGAAGAA AGAAGTTCTC CCACAAGAAA AACCAGGAGA ATTGAAAATA 
TCATTACCAG AAAAGAAAGA TATTCCTGAA ACAAAGGAAA CAGTCTTAGA GGAGGTGACA 
ATTCATGAAG CTGAAGACGA ATGCCCAAAG GAAGATTATG AACAAGTTAC ACCTCAAGCT 
ACTGAAAAAG AACCCCCAAA AGTCATGCAA GATCATTTAA CAGCTCATAA AGATACAGAA 
GAAAGTCCAA AAAAAGAGCA AGAACCCATG ACACCTCAGA TAGTAAAAGA TGGACCACCA 
AAACCAGTTG AAGCTGAAGT AAAAACAGAA GAGAAGAAAG AAGGAAGAAG CCAACAGCCT 
GCATTTACCG GTGTATCTAC AGATTTTGGC ATCATGCCTC GATTGATCAT AATCATGTCA 
GATTCACATG GTAGGTTTGC TTCAAGACCA GATGTCTCTG TAGATTGGAC CAAAAGAGAA 
ATGTTACTTA AAAAAGAGGA AAAAGAAGCT GAACCAATAA CACAAGAAAA GATGGACTAT 
ATAAAAGAAG CCCCAACTAC ATATATAATA GCAGAGGCTC CTGTGCAGAT TAATGTCACA 
AAACCACCAG ATGCAAAGAA AGAAATACAA ATGGAAATGC CTGTACGAAA ACTTGAAGAA 
GGACCAAAAG AAATAAAAAC AGAAATACCA GTGGAGATAC CTGAAGAAAA AACAAAAGAT 
GTAAAAGAAG TTGTAGTAAT ACCAGTGGAA ATGCCAGTGA AGAAAATTAA ACCGGAAGAA 
GAGATACCAG TGGAAATGCC AGTGAAAAAA CTTAAAGAAC CAGAAGAAAT ACCAGTGGAA 
ATACCAGTAG AGAAACCCAA AGAAGTATCA AAAGCAAAAG AACAAGAGGT GCCACTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCATTCAGAA GACTGAACAA 
GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAAAAAGAG 
GAACCAGTTA AGAAGGCTGA ACCAGAAGTA AAGGACATTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA 
AAACCTGCAG AAGTAAAGAA AGAGGTGCCC ATTAAGAAGA CTGAACCAGA AGTGAAGGAA 
GTTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCTGT AAAGAAACCT GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGTCC GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT 
GAACTGAAGA CACCAAAAGC CATTTATGAA GAAAAAACAA CCAAATCGAA TGATACAGCA 
AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902110.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757970.1 Xenopus tropicalis triadin (trdn), transcript variant X44, mRNA.

Price & Availability↑

CloneID	OXa58406
Clone ID Related Accession (Same CDS sequence)	XM_031902110.1
Accession Version	XM_031902110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X44
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGTCC 
GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT GAACTGAAGA CACCAAAAGC CATTTATGAA 
GAAAAAACAA CCAAATCGAA TGATACAGCA AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757970.1
CDS	384..4568
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902110.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X44, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902110.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGTCC 
GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT GAACTGAAGA CACCAAAAGC CATTTATGAA 
GAAAAAACAA CCAAATCGAA TGATACAGCA AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902111.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757971.1 Xenopus tropicalis triadin (trdn), transcript variant X45, mRNA.

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CloneID	OXa58407
Clone ID Related Accession (Same CDS sequence)	XM_031902111.1
Accession Version	XM_031902111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X45
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGTCC 
GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT GAACTGAAGA CACCAAAAGC CATTTATGAA 
GAAAAAACAA CCAAATCGAA TGATACAGCA AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757971.1
CDS	384..4568
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902111.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X45, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902111.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGTCC 
GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT GAACTGAAGA CACCAAAAGC CATTTATGAA 
GAAAAAACAA CCAAATCGAA TGATACAGCA AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902112.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757972.1 Xenopus tropicalis triadin (trdn), transcript variant X46, mRNA.

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CloneID	OXa58408
Clone ID Related Accession (Same CDS sequence)	XM_031902112.1
Accession Version	XM_031902112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X46
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGTCC 
GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT GAACTGAAGA CACCAAAAGC CATTTATGAA 
GAAAAAACAA CCAAATCGAA TGATACAGCA AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757972.1
CDS	384..4568
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902112.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X46, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902112.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGTCC 
GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT GAACTGAAGA CACCAAAAGC CATTTATGAA 
GAAAAAACAA CCAAATCGAA TGATACAGCA AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902113.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757973.1 Xenopus tropicalis triadin (trdn), transcript variant X47, mRNA.

Price & Availability↑

CloneID	OXa58409
Clone ID Related Accession (Same CDS sequence)	XM_031902113.1
Accession Version	XM_031902113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X47
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGTCC 
GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT GAACTGAAGA CACCAAAAGC CATTTATGAA 
GAAAAAACAA CCAAATCGAA TGATACAGCA AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757973.1
CDS	384..4568
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902113.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X47, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902113.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGTCC 
GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT GAACTGAAGA CACCAAAAGC CATTTATGAA 
GAAAAAACAA CCAAATCGAA TGATACAGCA AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902114.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757974.1 Xenopus tropicalis triadin (trdn), transcript variant X48, mRNA.

Price & Availability↑

CloneID	OXa58410
Clone ID Related Accession (Same CDS sequence)	XM_031902114.1
Accession Version	XM_031902114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X48
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGTCC 
GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT GAACTGAAGA CACCAAAAGC CATTTATGAA 
GAAAAAACAA CCAAATCGAA TGATACAGCA AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757974.1
CDS	384..4568
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902114.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X48, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902114.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGTCC 
GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT GAACTGAAGA CACCAAAAGC CATTTATGAA 
GAAAAAACAA CCAAATCGAA TGATACAGCA AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902115.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757975.1 Xenopus tropicalis triadin (trdn), transcript variant X49, mRNA.

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CloneID	OXa58411
Clone ID Related Accession (Same CDS sequence)	XM_031902115.1
Accession Version	XM_031902115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X49
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGTCC 
GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT GAACTGAAGA CACCAAAAGC CATTTATGAA 
GAAAAAACAA CCAAATCGAA TGATACAGCA AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757975.1
CDS	384..4568
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902115.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X49, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902115.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCCGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATATATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TCCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGCTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCACT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGTCC 
GAGCCAAAAC CTGCAGAAGT TAAGAAAGAT GAACTGAAGA CACCAAAAGC CATTTATGAA 
GAAAAAACAA CCAAATCGAA TGATACAGCA AATATGCTGT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_031902116.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757976.1 Xenopus tropicalis triadin (trdn), transcript variant X50, mRNA.

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CloneID	OXa58412
Clone ID Related Accession (Same CDS sequence)	XM_031902116.1
Accession Version	XM_031902116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X50
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGG AAGTTCACTT 
AACAAGTTCC GGAGTAATCC CGTCGGAATA ATACATGAAG CAGTGGATGA AACAACTGAT 
TGGATTTATG GAATATATAT TACTATATCT GACTTGATAT CCCTTGATGA AGAACGTAAA 
GGGCATGCTG AGCCTCCTGT AAAGAAAATA GGTGGAATAC AACCAGCTCT ACACAGAAAA 
GCAATACCAA AACTCAAGGC TGAAGAGGAA GATGAGCCAG TTAAGAAAAC CAGAGCAAAA 
GAAAAGGCTG AAACAAAAGA AAAAGTAGAG AAGAAAATTC TACCTAAAGC CTCACTAAAA 
GCAGAGAAGA AGGAGAAACC TGAAAAAATT ACCTTGACAA AATCTGAGAA GAAAGAAAAA 
TCTGCAATAA AACCAGAGAA AAAAGACAAG ACGGAAAAGG AAGCCTCAGT CAAAACTGAA 
AAGAAAGTAA AAGCTGCAAA ATTAGACAAA GTCAAAATTG ACAAGAAAGA AAAAGCTGAA 
AAACTTGTCA CACCCAAGTC TGAGAAGAAG GAAAAAACTG AAAAAGTAAC CCCATCCAAA 
CTTGAGAAAA AAGTAAAAGC TGAACAGATA GCTCCCACAA AACTAGAGAA AAAGGAAAAA 
ACTGAAAAGG TAGCAACCAC AAAATCAGAA AAGAAAGAAA AAGCTGAAAA GTTAACCCCA 
GTCAAAGTAG AGAAGACATT AAAACTTGAA AAGGTAGCAC CAGTTAAAGC TGAGAAAAAG 
GAAAAAGCTG AAAAAGTAGT CCCTGTTAAA GTTCAGAAAA AGGAAAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGAAAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGACAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTTCCAGTTA AAGCAGAGAA GAAAGGCAAA GCTGAAAAAG TAGCCCCTGT CAAACCCCAG 
AAGGAGAAAG CTGAAAAAGT AACCTCGGTC AAAGCTGAAA AGAAGGAAAA AGCTGAAAAA 
ATTGCCCCAG TAAAAGCCGA GAAGAAGGAA AAAGTTGAAA AGATAGCCAC AAGTAAAGCC 
GAGAAGAAGG TTGAAAAGTT AGCCCCAGTC AAACCTGAGA AAAAGGAAAA GGCTAAAAAA 
GTAGCCCCAG TCAAATCTGA GAAGAAGGAA AAGACTGAAA AGTTAACCCA AGACACAACC 
GAGAAGGTAA AAGCTGAAAA GGTTGCTCCA ACCAAATTGG AGAAGAAACA AAAAGCTGAA 
ACCAGGGCCC CAGTCAAAAC TGAGAAGAAG GACAAAGCAG AAAAGGAAAT CATACCACAA 
GAAGACAAAA AAGTCAAGGT TTCTCAGGTG GAAGAAAAGA CTAAGGATGT CAAAGCTAAA 
ACATCAGAGA GAAAAGTAGC AAAAGACTCA AAGAAAGACG CTAAAGAGGA AGAAGAAATC 
AAGACAAAGC CACATACTAA AGTGGAAACA CTGGATGCAG CAGAAACAAT GGGGGAAATA 
AAAGATCAGT ATAATTTCTG CCGTTATGTG GTTGACCTGT ATTCTCATGG AGATGCTTCT 
ACAGAAGAAA AATCAAAGGA AAAACCACAA GAGAAGAAAG AAGTTCTCCC ACAAGAAAAA 
CCAGGAGAAT TGAAAATATC ATTACCAGAA AAGAAAGATA TTCCTGAAAC AAAGGAAACA 
GTCTTAGAGG AGGTGACAAT TCATGAAGCT GAAGACGAAT GCCCAAAGGA AGATTATGAA 
CAAGTTACAC CTCAAGCTAC TGAAAAAGAA CCCCCAAAAG TCATGCAAGA TCATTTAACA 
GCTCATAAAG ATACAGAAGA AAGTCCAAAA AAAGAGCAAG AACCCATGAC ACCTCAGATA 
GTAAAAGATG GACCACCAAA ACCAGTTGAA GCTGAAGTAA AAACAGAAGA GAAGAAAGAA 
GGAAGAAGCC AACAGCCTGC ATTTACCGGT GTATCTACAG ATTTTGGCAT CATGCCTCGA 
TTGATCATAA TCATGTCAGA TTCACATGGT AGGTTTGCTT CAAGACCAGA TGTCTCTGTA 
GATTGGACCA AAAGAGAAAT GTTACTTAAA AAAGAGGAAA AAGAAGCTGA ACCAATAACA 
CAAGAAAAGA TGGACTATAT AAAAGAAGCC CCAACTACAT ATATAATAGC AGAGGCTCCT 
GTGCAGATTA ATGTCACAAA ACCACCAGAT GCAAAGAAAG AAATACAAAT GGAAATGCCT 
GTACGAAAAC TTGAAGAAGG ACCAAAAGAA ATAAAAACAG AAATACCAGT GGAGATACCT 
GAAGAAAAAA CAAAAGATGT AAAAGAAGTT GTAGTAATAC CAGTGGAAAT GCCAGTGAAG 
AAAATTAAAC CGGAAGAAGA GATACCAGTG GAAATGCCAG TGAAAAAACT TAAAGAACCA 
GAAGAAATAC CAGTGGAAAT ACCAGTAGAG AAACCCAAAG AAGTATCAAA AGCAAAAGAA 
CAAGAGGTGC CACTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTCAGAAGA CTGAACAAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757976.1
CDS	383..4555
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPPVKKIGGIQPALHRKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTAHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKSEPKPAEVKKDELKTPKAIYEEKTTKSNDTANMLS

Target ORF information:

RefSeq Version	XM_031902116.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X50, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902116.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGG AAGTTCACTT 
AACAAGTTCC GGAGTAATCC CGTCGGAATA ATACATGAAG CAGTGGATGA AACAACTGAT 
TGGATTTATG GAATATATAT TACTATATCT GACTTGATAT CCCTTGATGA AGAACGTAAA 
GGGCATGCTG AGCCTCCTGT AAAGAAAATA GGTGGAATAC AACCAGCTCT ACACAGAAAA 
GCAATACCAA AACTCAAGGC TGAAGAGGAA GATGAGCCAG TTAAGAAAAC CAGAGCAAAA 
GAAAAGGCTG AAACAAAAGA AAAAGTAGAG AAGAAAATTC TACCTAAAGC CTCACTAAAA 
GCAGAGAAGA AGGAGAAACC TGAAAAAATT ACCTTGACAA AATCTGAGAA GAAAGAAAAA 
TCTGCAATAA AACCAGAGAA AAAAGACAAG ACGGAAAAGG AAGCCTCAGT CAAAACTGAA 
AAGAAAGTAA AAGCTGCAAA ATTAGACAAA GTCAAAATTG ACAAGAAAGA AAAAGCTGAA 
AAACTTGTCA CACCCAAGTC TGAGAAGAAG GAAAAAACTG AAAAAGTAAC CCCATCCAAA 
CTTGAGAAAA AAGTAAAAGC TGAACAGATA GCTCCCACAA AACTAGAGAA AAAGGAAAAA 
ACTGAAAAGG TAGCAACCAC AAAATCAGAA AAGAAAGAAA AAGCTGAAAA GTTAACCCCA 
GTCAAAGTAG AGAAGACATT AAAACTTGAA AAGGTAGCAC CAGTTAAAGC TGAGAAAAAG 
GAAAAAGCTG AAAAAGTAGT CCCTGTTAAA GTTCAGAAAA AGGAAAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGAAAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGACAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTTCCAGTTA AAGCAGAGAA GAAAGGCAAA GCTGAAAAAG TAGCCCCTGT CAAACCCCAG 
AAGGAGAAAG CTGAAAAAGT AACCTCGGTC AAAGCTGAAA AGAAGGAAAA AGCTGAAAAA 
ATTGCCCCAG TAAAAGCCGA GAAGAAGGAA AAAGTTGAAA AGATAGCCAC AAGTAAAGCC 
GAGAAGAAGG TTGAAAAGTT AGCCCCAGTC AAACCTGAGA AAAAGGAAAA GGCTAAAAAA 
GTAGCCCCAG TCAAATCTGA GAAGAAGGAA AAGACTGAAA AGTTAACCCA AGACACAACC 
GAGAAGGTAA AAGCTGAAAA GGTTGCTCCA ACCAAATTGG AGAAGAAACA AAAAGCTGAA 
ACCAGGGCCC CAGTCAAAAC TGAGAAGAAG GACAAAGCAG AAAAGGAAAT CATACCACAA 
GAAGACAAAA AAGTCAAGGT TTCTCAGGTG GAAGAAAAGA CTAAGGATGT CAAAGCTAAA 
ACATCAGAGA GAAAAGTAGC AAAAGACTCA AAGAAAGACG CTAAAGAGGA AGAAGAAATC 
AAGACAAAGC CACATACTAA AGTGGAAACA CTGGATGCAG CAGAAACAAT GGGGGAAATA 
AAAGATCAGT ATAATTTCTG CCGTTATGTG GTTGACCTGT ATTCTCATGG AGATGCTTCT 
ACAGAAGAAA AATCAAAGGA AAAACCACAA GAGAAGAAAG AAGTTCTCCC ACAAGAAAAA 
CCAGGAGAAT TGAAAATATC ATTACCAGAA AAGAAAGATA TTCCTGAAAC AAAGGAAACA 
GTCTTAGAGG AGGTGACAAT TCATGAAGCT GAAGACGAAT GCCCAAAGGA AGATTATGAA 
CAAGTTACAC CTCAAGCTAC TGAAAAAGAA CCCCCAAAAG TCATGCAAGA TCATTTAACA 
GCTCATAAAG ATACAGAAGA AAGTCCAAAA AAAGAGCAAG AACCCATGAC ACCTCAGATA 
GTAAAAGATG GACCACCAAA ACCAGTTGAA GCTGAAGTAA AAACAGAAGA GAAGAAAGAA 
GGAAGAAGCC AACAGCCTGC ATTTACCGGT GTATCTACAG ATTTTGGCAT CATGCCTCGA 
TTGATCATAA TCATGTCAGA TTCACATGGT AGGTTTGCTT CAAGACCAGA TGTCTCTGTA 
GATTGGACCA AAAGAGAAAT GTTACTTAAA AAAGAGGAAA AAGAAGCTGA ACCAATAACA 
CAAGAAAAGA TGGACTATAT AAAAGAAGCC CCAACTACAT ATATAATAGC AGAGGCTCCT 
GTGCAGATTA ATGTCACAAA ACCACCAGAT GCAAAGAAAG AAATACAAAT GGAAATGCCT 
GTACGAAAAC TTGAAGAAGG ACCAAAAGAA ATAAAAACAG AAATACCAGT GGAGATACCT 
GAAGAAAAAA CAAAAGATGT AAAAGAAGTT GTAGTAATAC CAGTGGAAAT GCCAGTGAAG 
AAAATTAAAC CGGAAGAAGA GATACCAGTG GAAATGCCAG TGAAAAAACT TAAAGAACCA 
GAAGAAATAC CAGTGGAAAT ACCAGTAGAG AAACCCAAAG AAGTATCAAA AGCAAAAGAA 
CAAGAGGTGC CACTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTCAGAAGA CTGAACAAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGTCCGA GCCAAAACCT 
GCAGAAGTTA AGAAAGATGA ACTGAAGACA CCAAAAGCCA TTTATGAAGA AAAAACAACC 
AAATCGAATG ATACAGCAAA TATGCTGTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963609.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819063.1 Xenopus tropicalis triadin (trdn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31743
Clone ID Related Accession (Same CDS sequence)	XM_012963609.2
Accession Version	XM_012963609.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963609.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGGTAA ATATTGTAGG CTCTGTAAAA CTCTGTCTAT AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819063.1
CDS	391..5187
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963609.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963609.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGGTAA ATATTGTAGG CTCTGTAAAA CTCTGTCTAT AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963610.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819064.1 Xenopus tropicalis triadin (trdn), transcript variant X2, mRNA.

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CloneID	OXa31744
Clone ID Related Accession (Same CDS sequence)	XM_012963610.2
Accession Version	XM_012963610.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963610.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGGTAA ATATTGTAGG CTCTGTAAAA CTCTGTCTAT AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819064.1
CDS	391..5187
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963610.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963610.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGGTAA ATATTGTAGG CTCTGTAAAA CTCTGTCTAT AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963612.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819066.1 Xenopus tropicalis triadin (trdn), transcript variant X3, mRNA.

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CloneID	OXa31746
Clone ID Related Accession (Same CDS sequence)	XM_012963612.2
Accession Version	XM_012963612.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963612.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819066.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963612.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963612.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963613.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819067.1 Xenopus tropicalis triadin (trdn), transcript variant X4, mRNA.

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CloneID	OXa31747
Clone ID Related Accession (Same CDS sequence)	XM_012963613.2
Accession Version	XM_012963613.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963613.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819067.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963613.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963613.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963615.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819069.1 Xenopus tropicalis triadin (trdn), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa31749
Clone ID Related Accession (Same CDS sequence)	XM_012963615.2
Accession Version	XM_012963615.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963615.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCCAT TCAGAAGACT 
GAACAAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAGGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819069.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963615.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963615.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCCAT TCAGAAGACT 
GAACAAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAGGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963616.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819070.1 Xenopus tropicalis triadin (trdn), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa31750
Clone ID Related Accession (Same CDS sequence)	XM_012963616.2
Accession Version	XM_012963616.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963616.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819070.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963616.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963616.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963617.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819071.1 Xenopus tropicalis triadin (trdn), transcript variant X7, mRNA.

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CloneID	OXa31751
Clone ID Related Accession (Same CDS sequence)	XM_012963617.2
Accession Version	XM_012963617.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAGGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819071.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963617.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963617.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAGGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963618.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819072.1 Xenopus tropicalis triadin (trdn), transcript variant X8, mRNA.

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CloneID	OXa31752
Clone ID Related Accession (Same CDS sequence)	XM_012963618.2
Accession Version	XM_012963618.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCCAT TCAGAAGACT 
GAACAAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819072.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963618.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963618.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCCAT TCAGAAGACT 
GAACAAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963620.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819074.1 Xenopus tropicalis triadin (trdn), transcript variant X9, mRNA.

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CloneID	OXa31754
Clone ID Related Accession (Same CDS sequence)	XM_012963620.2
Accession Version	XM_012963620.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963620.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819074.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963620.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963620.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963621.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819075.1 Xenopus tropicalis triadin (trdn), transcript variant X10, mRNA.

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CloneID	OXa31755
Clone ID Related Accession (Same CDS sequence)	XM_012963621.2
Accession Version	XM_012963621.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819075.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963621.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963621.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGATGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963622.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819076.1 Xenopus tropicalis triadin (trdn), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OXa31756
Clone ID Related Accession (Same CDS sequence)	XM_012963622.2
Accession Version	XM_012963622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819076.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963622.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963622.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963623.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819077.1 Xenopus tropicalis triadin (trdn), transcript variant X12, mRNA.

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CloneID	OXa31757
Clone ID Related Accession (Same CDS sequence)	XM_012963623.2
Accession Version	XM_012963623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963623.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819077.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963623.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963623.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963624.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819078.1 Xenopus tropicalis triadin (trdn), transcript variant X16, mRNA.

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CloneID	OXa31758
Clone ID Related Accession (Same CDS sequence)	XM_012963624.2
Accession Version	XM_012963624.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963624.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819078.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963624.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963624.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963625.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819079.1 Xenopus tropicalis triadin (trdn), transcript variant X13, mRNA.

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CloneID	OXa31759
Clone ID Related Accession (Same CDS sequence)	XM_012963625.2
Accession Version	XM_012963625.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819079.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963625.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963625.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963626.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819080.1 Xenopus tropicalis triadin (trdn), transcript variant X14, mRNA.

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CloneID	OXa31760
Clone ID Related Accession (Same CDS sequence)	XM_012963626.2
Accession Version	XM_012963626.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819080.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963626.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963626.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963627.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819081.1 Xenopus tropicalis triadin (trdn), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OXa31761
Clone ID Related Accession (Same CDS sequence)	XM_012963627.2
Accession Version	XM_012963627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819081.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963627.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963627.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963629.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819083.1 Xenopus tropicalis triadin (trdn), transcript variant X17, mRNA.

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CloneID	OXa31763
Clone ID Related Accession (Same CDS sequence)	XM_012963629.2
Accession Version	XM_012963629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819083.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963629.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963629.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963630.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819084.1 Xenopus tropicalis triadin (trdn), transcript variant X18, mRNA.

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CloneID	OXa31764
Clone ID Related Accession (Same CDS sequence)	XM_012963630.2
Accession Version	XM_012963630.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963630.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819084.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963630.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963630.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963631.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819085.1 Xenopus tropicalis triadin (trdn), transcript variant X19, mRNA.

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CloneID	OXa31765
Clone ID Related Accession (Same CDS sequence)	XM_012963631.2
Accession Version	XM_012963631.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963631.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819085.1
CDS	391..5139
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963631.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963631.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGGT AAATATTGTA GGCTCTGTAA AACTCTGTCT 
ATAAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963632.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819086.1 Xenopus tropicalis triadin (trdn), transcript variant X20, mRNA.

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CloneID	OXa31766
Clone ID Related Accession (Same CDS sequence)	XM_012963632.2
Accession Version	XM_012963632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGAGATAC CAGTGGAAAT GCCAGTGAAA AAACTTAAAG AACCAGAAGA AATACCAGTG 
GAAATACCAG TAGAGAAACC CAAAGAAGTA TCAAAAGCAA AAGAACAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCATTCA GAAGACTGAA 
CAAGAAGTGA AGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAGGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCCTTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAAA AAGAGGAACC AGTTAAGAAG GCTGAACCAG AAGTAAAGGA CATTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCTGTA 
AAGAAACCTG AAGCAAAACC TGCAGAAGTA AAGAAAGAGG TGCCCATTAA GAAGACTGAA 
CCAGAAGTGA TGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC CTTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAAAAA 
GAGGAACCAG TTAAGAAGGC TGAACCAGAA GTAAAGGACA TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCTGTAAA GAAACCTGAA 
GCAAAACCTG CAGAAGTAAA GAAAGAGGTG CCCATTAAGA AGACTGAACC AGAAGTGAAG 
GAAGTTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAAAAAGA GGAACCAGTT 
AAGAAGGCTG AACCAGAAGT AAAGGACATT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCTGTAAAGA AACCTGAAGC AAAACCTGCA 
GAAGTAAAGA AAGAGGTGCC CATTAAGAAG ACTGAACCAG AAGTGAAGGA AGTTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGGTAAA TATTGTAGGC TCTGTAAAAC TCTGTCTATA 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819086.1
CDS	391..5136
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963632.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963632.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGAGATAC CAGTGGAAAT GCCAGTGAAA AAACTTAAAG AACCAGAAGA AATACCAGTG 
GAAATACCAG TAGAGAAACC CAAAGAAGTA TCAAAAGCAA AAGAACAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCATTCA GAAGACTGAA 
CAAGAAGTGA AGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAGGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCCTTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAAA AAGAGGAACC AGTTAAGAAG GCTGAACCAG AAGTAAAGGA CATTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCTGTA 
AAGAAACCTG AAGCAAAACC TGCAGAAGTA AAGAAAGAGG TGCCCATTAA GAAGACTGAA 
CCAGAAGTGA TGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC CTTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAAAAA 
GAGGAACCAG TTAAGAAGGC TGAACCAGAA GTAAAGGACA TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCTGTAAA GAAACCTGAA 
GCAAAACCTG CAGAAGTAAA GAAAGAGGTG CCCATTAAGA AGACTGAACC AGAAGTGAAG 
GAAGTTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAAAAAGA GGAACCAGTT 
AAGAAGGCTG AACCAGAAGT AAAGGACATT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCTGTAAAGA AACCTGAAGC AAAACCTGCA 
GAAGTAAAGA AAGAGGTGCC CATTAAGAAG ACTGAACCAG AAGTGAAGGA AGTTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGGTAAA TATTGTAGGC TCTGTAAAAC TCTGTCTATA 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963633.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819087.1 Xenopus tropicalis triadin (trdn), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OXa31767
Clone ID Related Accession (Same CDS sequence)	XM_012963633.2
Accession Version	XM_012963633.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963633.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGAAGTTCAC TTAACAAGTT CCGGAGTAAT 
CCTGTCGGAA TAATACATGA AGCAGTGGAT GAAACAACTG ATTGGATTTA TGGAATATAC 
ATTACTATAT CTGACTTGAT ATCCCTTGAT GAAGAACGTA AAGGGCATGC TGAGCCTTCT 
GTAAAGAAAA TAGGTGGAAT ACAACCAGCT CTACACAGAA AAGTAATTCA TAAAGAGAAA 
CCTGAAGTAA AAGAGAACAT TTTGAAAAAG GCCTCAGCTA AAGCAATACC AAAACTCAAG 
GCTGAAGAGG AAGATGAGCC AGTTAAGAAA ACCAGAGCAA AAGAAAAGGC TGAAACAAAA 
GAAAAAGTAG AGAAGAAAAT TCTACCTAAA GCCTCACTAA AAGCAGAGAA GAAGGAGAAA 
CCTGAAAAAA TTACCTTGAC AAAATCTGAG AAGAAAGAAA AATCTGCAAT AAAACCAGAG 
AAAAAAGACA AGACGGAAAA GGAAGCCTCA GTCAAAACTG AAAAGAAAGT AAAAGCTGCA 
AAATTAGACA AAGTCAAAAT TGACAAGAAA GAAAAAGCTG AAAAACTTGT CACACCCAAG 
TCTGAGAAGA AGGAAAAAAC TGAAAAAGTA ACCCCATCCA AACTTGAGAA AAAAGTAAAA 
GCTGAACAGA TAGCTCCCAC AAAACTAGAG AAAAAGGAAA AAACTGAAAA GGTAGCAACC 
ACAAAATCAG AAAAGAAAGA AAAAGCTGAA AAGTTAACCC CAGTCAAAGT AGAGAAGACA 
TTAAAACTTG AAAAGGTAGC ACCAGTTAAA GCTGAGAAAA AGGAAAAAGC TGAAAAAGTA 
GTCCCTGTTA AAGTTCAGAA AAAGGAAAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGAAA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGACA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGAAA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGACA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTTCCAGT TAAAGCAGAG 
AAGAAAGGCA AAGCTGAAAA AGTAGCCCCT GTCAAACCCC AGAAGGAGAA AGCTGAAAAA 
GTAACCTCGG TCAAAGCTGA AAAGAAGGAA AAAGCTGAAA AAATTGCCCC AGTAAAAGCC 
GAGAAGAAGG AAAAAGTTGA AAAGATAGCC ACAAGTAAAG CCGAGAAGAA GGTTGAAAAG 
TTAGCCCCAG TCAAACCTGA GAAAAAGGAA AAGGCTAAAA AAGTAGCCCC AGTCAAATCT 
GAGAAGAAGG AAAAGACTGA AAAGTTAACC CAAGACACAA CCGAGAAGGT AAAAGCTGAA 
AAGGTTGCTC CAACCAAATT GGAGAAGAAA CAAAAAGCTG AAACCAGGGC CCCAGTCAAA 
ACTGAGAAGA AGGACAAAGC AGAAAAGGAA ATCATACCAC AAGAAGACAA AAAAGTCAAG 
GTTTCTCAGG TGGAAGAAAA GACTAAGGAT GTCAAAGCTA AAACATCAGA GAGAAAAGTA 
GCAAAAGACT CAAAGAAAGA CGCTAAAGAG GAAGAAGAAA TCAAGACAAA GCCACATACT 
AAAGTGGAAA CACTGGATGC AGCAGAAACA ATGGGGGAAA TAAAAGATCA GTATAATTTC 
TGCCGTTATG TGGTTGACCT GTATTCTCAT GGAGATGCTT CTACAGAAGA AAAATCAAAG 
GAAAAACCAC AAGAGAAGAA AGAAGTTCTC CCACAAGAAA AACCAGGAGA ATTGAAAATA 
TCATTACCAG AAAAGAAAGA TATTCCTGAA ACAAAGGAAA CAGTCTTAGA GGAGGTGACA 
ATTCATGAAG CTGAAGACGA ATGCCCAAAG GAAGATTATG AACAAGTTAC ACCTCAAGCT 
ACTGAAAAAG AACCCCCAAA AGTCATGCAA GATCATTTAA CAGTTCATAA AGATACAGAA 
GAAAGTCCAA AAAAAGAGCA AGAACCCATG ACACCTCAGA TAGTAAAAGA TGGACCACCA 
AAACCAGTTG AAGCTGAAGT AAAAACAGAA GAGAAGAAAG AAGGAAGAAG CCAACAGCCT 
GCATTTACCG GTGTATCTAC AGATTTTGGC ATCATGCCTC GATTGATCAT AATCATGTCA 
GATTCACATG GTAGGTTTGC TTCAAGACCA GATGTCTCTG TAGATTGGAC CAAAAGAGAA 
ATGTTACTTA AAAAAGAGGA AAAAGAAGCT GAACCAATAA CACAAGAAAA GATGGACTAT 
ATAAAAGAAG CCCCAACTAC ATATATAATA GCAGAGGCTC CTGTGCAGAT TAATGTCACA 
AAACCACCAG ATGCAAAGAA AGAAATACAA ATGGAAATGC CTGTACGAAA ACTTGAAGAA 
GGACCAAAAG AAATAAAAAC AGAAATACCA GTGGAGATAC CTGAAGAAAA AACAAAAGAT 
GTAAAAGAAG TTGTAGTAAT ACCAGTGGAA ATGCCAGTGA AGAAAATTAA ACCGGAAGAA 
GAGATACCAG TGGAAATGCC AGTGAAAAAA CTTAAAGAAC CAGAAGAAAT ACCAGTGGAA 
ATACCAGTAG AGAAACCCAA AGAAGTATCA AAAGCAAAAG AACAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCATTCAGAA GACTGAACAA 
GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAG 
GTTAAGAAAG AGGTGCCTGT AAAGAAACCT GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCTGT AAAGAAACCT GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCC TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAAAAAG AGGAACCAGT TAAGAAGGCT GAACCAGAAG TAAAGGACAT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG 
AAACCTGAAG CAAAACCTGC AGAAGTAAAG AAAGAGGTGC CCATTAAGAA GACTGAACCA 
GAAGTGATGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAAAAAGAG 
GAACCAGTTA AGAAGGCTGA ACCAGAAGTA AAGGACATTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA 
AAACCTGCAG AAGTAAAGAA AGAGGTGCCC ATTAAGAAGA CTGAACCAGA AGTGAAGGAA 
GTTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCTGT AAAGAAACCT GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGGTAAATAT TGTAGGCTCT GTAAAACTCT GTCTATAAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819087.1
CDS	391..5133
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963633.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963633.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGAAGTTCAC TTAACAAGTT CCGGAGTAAT 
CCTGTCGGAA TAATACATGA AGCAGTGGAT GAAACAACTG ATTGGATTTA TGGAATATAC 
ATTACTATAT CTGACTTGAT ATCCCTTGAT GAAGAACGTA AAGGGCATGC TGAGCCTTCT 
GTAAAGAAAA TAGGTGGAAT ACAACCAGCT CTACACAGAA AAGTAATTCA TAAAGAGAAA 
CCTGAAGTAA AAGAGAACAT TTTGAAAAAG GCCTCAGCTA AAGCAATACC AAAACTCAAG 
GCTGAAGAGG AAGATGAGCC AGTTAAGAAA ACCAGAGCAA AAGAAAAGGC TGAAACAAAA 
GAAAAAGTAG AGAAGAAAAT TCTACCTAAA GCCTCACTAA AAGCAGAGAA GAAGGAGAAA 
CCTGAAAAAA TTACCTTGAC AAAATCTGAG AAGAAAGAAA AATCTGCAAT AAAACCAGAG 
AAAAAAGACA AGACGGAAAA GGAAGCCTCA GTCAAAACTG AAAAGAAAGT AAAAGCTGCA 
AAATTAGACA AAGTCAAAAT TGACAAGAAA GAAAAAGCTG AAAAACTTGT CACACCCAAG 
TCTGAGAAGA AGGAAAAAAC TGAAAAAGTA ACCCCATCCA AACTTGAGAA AAAAGTAAAA 
GCTGAACAGA TAGCTCCCAC AAAACTAGAG AAAAAGGAAA AAACTGAAAA GGTAGCAACC 
ACAAAATCAG AAAAGAAAGA AAAAGCTGAA AAGTTAACCC CAGTCAAAGT AGAGAAGACA 
TTAAAACTTG AAAAGGTAGC ACCAGTTAAA GCTGAGAAAA AGGAAAAAGC TGAAAAAGTA 
GTCCCTGTTA AAGTTCAGAA AAAGGAAAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGAAA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGACA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGAAA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTCCCTGT TAAAGATGAG 
AAAAAGGACA AAGCTGAAAA AAAGCAGAAA GCTGAAAAGG TAGTTCCAGT TAAAGCAGAG 
AAGAAAGGCA AAGCTGAAAA AGTAGCCCCT GTCAAACCCC AGAAGGAGAA AGCTGAAAAA 
GTAACCTCGG TCAAAGCTGA AAAGAAGGAA AAAGCTGAAA AAATTGCCCC AGTAAAAGCC 
GAGAAGAAGG AAAAAGTTGA AAAGATAGCC ACAAGTAAAG CCGAGAAGAA GGTTGAAAAG 
TTAGCCCCAG TCAAACCTGA GAAAAAGGAA AAGGCTAAAA AAGTAGCCCC AGTCAAATCT 
GAGAAGAAGG AAAAGACTGA AAAGTTAACC CAAGACACAA CCGAGAAGGT AAAAGCTGAA 
AAGGTTGCTC CAACCAAATT GGAGAAGAAA CAAAAAGCTG AAACCAGGGC CCCAGTCAAA 
ACTGAGAAGA AGGACAAAGC AGAAAAGGAA ATCATACCAC AAGAAGACAA AAAAGTCAAG 
GTTTCTCAGG TGGAAGAAAA GACTAAGGAT GTCAAAGCTA AAACATCAGA GAGAAAAGTA 
GCAAAAGACT CAAAGAAAGA CGCTAAAGAG GAAGAAGAAA TCAAGACAAA GCCACATACT 
AAAGTGGAAA CACTGGATGC AGCAGAAACA ATGGGGGAAA TAAAAGATCA GTATAATTTC 
TGCCGTTATG TGGTTGACCT GTATTCTCAT GGAGATGCTT CTACAGAAGA AAAATCAAAG 
GAAAAACCAC AAGAGAAGAA AGAAGTTCTC CCACAAGAAA AACCAGGAGA ATTGAAAATA 
TCATTACCAG AAAAGAAAGA TATTCCTGAA ACAAAGGAAA CAGTCTTAGA GGAGGTGACA 
ATTCATGAAG CTGAAGACGA ATGCCCAAAG GAAGATTATG AACAAGTTAC ACCTCAAGCT 
ACTGAAAAAG AACCCCCAAA AGTCATGCAA GATCATTTAA CAGTTCATAA AGATACAGAA 
GAAAGTCCAA AAAAAGAGCA AGAACCCATG ACACCTCAGA TAGTAAAAGA TGGACCACCA 
AAACCAGTTG AAGCTGAAGT AAAAACAGAA GAGAAGAAAG AAGGAAGAAG CCAACAGCCT 
GCATTTACCG GTGTATCTAC AGATTTTGGC ATCATGCCTC GATTGATCAT AATCATGTCA 
GATTCACATG GTAGGTTTGC TTCAAGACCA GATGTCTCTG TAGATTGGAC CAAAAGAGAA 
ATGTTACTTA AAAAAGAGGA AAAAGAAGCT GAACCAATAA CACAAGAAAA GATGGACTAT 
ATAAAAGAAG CCCCAACTAC ATATATAATA GCAGAGGCTC CTGTGCAGAT TAATGTCACA 
AAACCACCAG ATGCAAAGAA AGAAATACAA ATGGAAATGC CTGTACGAAA ACTTGAAGAA 
GGACCAAAAG AAATAAAAAC AGAAATACCA GTGGAGATAC CTGAAGAAAA AACAAAAGAT 
GTAAAAGAAG TTGTAGTAAT ACCAGTGGAA ATGCCAGTGA AGAAAATTAA ACCGGAAGAA 
GAGATACCAG TGGAAATGCC AGTGAAAAAA CTTAAAGAAC CAGAAGAAAT ACCAGTGGAA 
ATACCAGTAG AGAAACCCAA AGAAGTATCA AAAGCAAAAG AACAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCATTCAGAA GACTGAACAA 
GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAG 
GTTAAGAAAG AGGTGCCTGT AAAGAAACCT GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCTGT AAAGAAACCT GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCC TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAAAAAG AGGAACCAGT TAAGAAGGCT GAACCAGAAG TAAAGGACAT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG 
AAACCTGAAG CAAAACCTGC AGAAGTAAAG AAAGAGGTGC CCATTAAGAA GACTGAACCA 
GAAGTGATGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAAAAAGAG 
GAACCAGTTA AGAAGGCTGA ACCAGAAGTA AAGGACATTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA 
AAACCTGCAG AAGTAAAGAA AGAGGTGCCC ATTAAGAAGA CTGAACCAGA AGTGAAGGAA 
GTTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGAGGAAC CAGTTAAGAA GGCTGAACCA 
GAAGTAAAGG ACATTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCTGT AAAGAAACCT GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG 
GTGCCCATTA AGAAGACTGA ACCAGAAGTG AAGGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGGTAAATAT TGTAGGCTCT GTAAAACTCT GTCTATAAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963635.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819089.1 Xenopus tropicalis triadin (trdn), transcript variant X24, mRNA.

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CloneID	OXa31769
Clone ID Related Accession (Same CDS sequence)	XM_012963635.2
Accession Version	XM_012963635.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963635.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGGTAA ATATTGTAGG CTCTGTAAAA CTCTGTCTAT AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819089.1
CDS	391..5127
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963635.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963635.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGGTAA ATATTGTAGG CTCTGTAAAA CTCTGTCTAT AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963637.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819091.1 Xenopus tropicalis triadin (trdn), transcript variant X25, mRNA.

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CloneID	OXa31771
Clone ID Related Accession (Same CDS sequence)	XM_012963637.2
Accession Version	XM_012963637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGG AAGTTCACTT 
AACAAGTTCC GGAGTAATCC TGTCGGAATA ATACATGAAG CAGTGGATGA AACAACTGAT 
TGGATTTATG GAATATACAT TACTATATCT GACTTGATAT CCCTTGATGA AGAACGTAAA 
GGGCATGCTG AGCCTTCTGT AAAGAAAATA GGTGGAATAC AACCAGCTCT ACACAGAAAA 
GTAATTCATA AAGAGAAACC TGAAGTAAAA GAGAACATTT TGAAAAAGGC CTCAGCTAAA 
GCAATACCAA AACTCAAGGC TGAAGAGGAA GATGAGCCAG TTAAGAAAAC CAGAGCAAAA 
GAAAAGGCTG AAACAAAAGA AAAAGTAGAG AAGAAAATTC TACCTAAAGC CTCACTAAAA 
GCAGAGAAGA AGGAGAAACC TGAAAAAATT ACCTTGACAA AATCTGAGAA GAAAGAAAAA 
TCTGCAATAA AACCAGAGAA AAAAGACAAG ACGGAAAAGG AAGCCTCAGT CAAAACTGAA 
AAGAAAGTAA AAGCTGCAAA ATTAGACAAA GTCAAAATTG ACAAGAAAGA AAAAGCTGAA 
AAACTTGTCA CACCCAAGTC TGAGAAGAAG GAAAAAACTG AAAAAGTAAC CCCATCCAAA 
CTTGAGAAAA AAGTAAAAGC TGAACAGATA GCTCCCACAA AACTAGAGAA AAAGGAAAAA 
ACTGAAAAGG TAGCAACCAC AAAATCAGAA AAGAAAGAAA AAGCTGAAAA GTTAACCCCA 
GTCAAAGTAG AGAAGACATT AAAACTTGAA AAGGTAGCAC CAGTTAAAGC TGAGAAAAAG 
GAAAAAGCTG AAAAAGTAGT CCCTGTTAAA GTTCAGAAAA AGGAAAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGAAAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGACAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGAAAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGACAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTTCCAGTTA AAGCAGAGAA GAAAGGCAAA GCTGAAAAAG TAGCCCCTGT CAAACCCCAG 
AAGGAGAAAG CTGAAAAAGT AACCTCGGTC AAAGCTGAAA AGAAGGAAAA AGCTGAAAAA 
ATTGCCCCAG TAAAAGCCGA GAAGAAGGAA AAAGTTGAAA AGATAGCCAC AAGTAAAGCC 
GAGAAGAAGG TTGAAAAGTT AGCCCCAGTC AAACCTGAGA AAAAGGAAAA GGCTAAAAAA 
GTAGCCCCAG TCAAATCTGA GAAGAAGGAA AAGACTGAAA AGTTAACCCA AGACACAACC 
GAGAAGGTAA AAGCTGAAAA GGTTGCTCCA ACCAAATTGG AGAAGAAACA AAAAGCTGAA 
ACCAGGGCCC CAGTCAAAAC TGAGAAGAAG GACAAAGCAG AAAAGGAAAT CATACCACAA 
GAAGACAAAA AAGTCAAGGT TTCTCAGGTG GAAGAAAAGA CTAAGGATGT CAAAGCTAAA 
ACATCAGAGA GAAAAGTAGC AAAAGACTCA AAGAAAGACG CTAAAGAGGA AGAAGAAATC 
AAGACAAAGC CACATACTAA AGTGGAAACA CTGGATGCAG CAGAAACAAT GGGGGAAATA 
AAAGATCAGT ATAATTTCTG CCGTTATGTG GTTGACCTGT ATTCTCATGG AGATGCTTCT 
ACAGAAGAAA AATCAAAGGA AAAACCACAA GAGAAGAAAG AAGTTCTCCC ACAAGAAAAA 
CCAGGAGAAT TGAAAATATC ATTACCAGAA AAGAAAGATA TTCCTGAAAC AAAGGAAACA 
GTCTTAGAGG AGGTGACAAT TCATGAAGCT GAAGACGAAT GCCCAAAGGA AGATTATGAA 
CAAGTTACAC CTCAAGCTAC TGAAAAAGAA CCCCCAAAAG TCATGCAAGA TCATTTAACA 
GTTCATAAAG ATACAGAAGA AAGTCCAAAA AAAGAGCAAG AACCCATGAC ACCTCAGATA 
GTAAAAGATG GACCACCAAA ACCAGTTGAA GCTGAAGTAA AAACAGAAGA GAAGAAAGAA 
GGAAGAAGCC AACAGCCTGC ATTTACCGGT GTATCTACAG ATTTTGGCAT CATGCCTCGA 
TTGATCATAA TCATGTCAGA TTCACATGGT AGGTTTGCTT CAAGACCAGA TGTCTCTGTA 
GATTGGACCA AAAGAGAAAT GTTACTTAAA AAAGAGGAAA AAGAAGCTGA ACCAATAACA 
CAAGAAAAGA TGGACTATAT AAAAGAAGCC CCAACTACAT ATATAATAGC AGAGGCTCCT 
GTGCAGATTA ATGTCACAAA ACCACCAGAT GCAAAGAAAG AAATACAAAT GGAAATGCCT 
GTACGAAAAC TTGAAGAAGG ACCAAAAGAA ATAAAAACAG AAATACCAGT GGAGATACCT 
GAAGAAAAAA CAAAAGATGT AAAAGAAGTT GTAGTAATAC CAGTGGAAAT GCCAGTGAAG 
AAAATTAAAC CGGAAGAAGA GATACCAGTG GAAATGCCAG TGAAAAAACT TAAAGAACCA 
GAAGAAATAC CAGTGGAAAT ACCAGTAGAG AAACCCAAAG AAGTATCAAA AGCAAAAGAA 
CAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTCAGAAGA CTGAACAAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAGGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819091.1
CDS	391..5091
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963637.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963637.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGG AAGTTCACTT 
AACAAGTTCC GGAGTAATCC TGTCGGAATA ATACATGAAG CAGTGGATGA AACAACTGAT 
TGGATTTATG GAATATACAT TACTATATCT GACTTGATAT CCCTTGATGA AGAACGTAAA 
GGGCATGCTG AGCCTTCTGT AAAGAAAATA GGTGGAATAC AACCAGCTCT ACACAGAAAA 
GTAATTCATA AAGAGAAACC TGAAGTAAAA GAGAACATTT TGAAAAAGGC CTCAGCTAAA 
GCAATACCAA AACTCAAGGC TGAAGAGGAA GATGAGCCAG TTAAGAAAAC CAGAGCAAAA 
GAAAAGGCTG AAACAAAAGA AAAAGTAGAG AAGAAAATTC TACCTAAAGC CTCACTAAAA 
GCAGAGAAGA AGGAGAAACC TGAAAAAATT ACCTTGACAA AATCTGAGAA GAAAGAAAAA 
TCTGCAATAA AACCAGAGAA AAAAGACAAG ACGGAAAAGG AAGCCTCAGT CAAAACTGAA 
AAGAAAGTAA AAGCTGCAAA ATTAGACAAA GTCAAAATTG ACAAGAAAGA AAAAGCTGAA 
AAACTTGTCA CACCCAAGTC TGAGAAGAAG GAAAAAACTG AAAAAGTAAC CCCATCCAAA 
CTTGAGAAAA AAGTAAAAGC TGAACAGATA GCTCCCACAA AACTAGAGAA AAAGGAAAAA 
ACTGAAAAGG TAGCAACCAC AAAATCAGAA AAGAAAGAAA AAGCTGAAAA GTTAACCCCA 
GTCAAAGTAG AGAAGACATT AAAACTTGAA AAGGTAGCAC CAGTTAAAGC TGAGAAAAAG 
GAAAAAGCTG AAAAAGTAGT CCCTGTTAAA GTTCAGAAAA AGGAAAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGAAAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGACAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGAAAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGACAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTTCCAGTTA AAGCAGAGAA GAAAGGCAAA GCTGAAAAAG TAGCCCCTGT CAAACCCCAG 
AAGGAGAAAG CTGAAAAAGT AACCTCGGTC AAAGCTGAAA AGAAGGAAAA AGCTGAAAAA 
ATTGCCCCAG TAAAAGCCGA GAAGAAGGAA AAAGTTGAAA AGATAGCCAC AAGTAAAGCC 
GAGAAGAAGG TTGAAAAGTT AGCCCCAGTC AAACCTGAGA AAAAGGAAAA GGCTAAAAAA 
GTAGCCCCAG TCAAATCTGA GAAGAAGGAA AAGACTGAAA AGTTAACCCA AGACACAACC 
GAGAAGGTAA AAGCTGAAAA GGTTGCTCCA ACCAAATTGG AGAAGAAACA AAAAGCTGAA 
ACCAGGGCCC CAGTCAAAAC TGAGAAGAAG GACAAAGCAG AAAAGGAAAT CATACCACAA 
GAAGACAAAA AAGTCAAGGT TTCTCAGGTG GAAGAAAAGA CTAAGGATGT CAAAGCTAAA 
ACATCAGAGA GAAAAGTAGC AAAAGACTCA AAGAAAGACG CTAAAGAGGA AGAAGAAATC 
AAGACAAAGC CACATACTAA AGTGGAAACA CTGGATGCAG CAGAAACAAT GGGGGAAATA 
AAAGATCAGT ATAATTTCTG CCGTTATGTG GTTGACCTGT ATTCTCATGG AGATGCTTCT 
ACAGAAGAAA AATCAAAGGA AAAACCACAA GAGAAGAAAG AAGTTCTCCC ACAAGAAAAA 
CCAGGAGAAT TGAAAATATC ATTACCAGAA AAGAAAGATA TTCCTGAAAC AAAGGAAACA 
GTCTTAGAGG AGGTGACAAT TCATGAAGCT GAAGACGAAT GCCCAAAGGA AGATTATGAA 
CAAGTTACAC CTCAAGCTAC TGAAAAAGAA CCCCCAAAAG TCATGCAAGA TCATTTAACA 
GTTCATAAAG ATACAGAAGA AAGTCCAAAA AAAGAGCAAG AACCCATGAC ACCTCAGATA 
GTAAAAGATG GACCACCAAA ACCAGTTGAA GCTGAAGTAA AAACAGAAGA GAAGAAAGAA 
GGAAGAAGCC AACAGCCTGC ATTTACCGGT GTATCTACAG ATTTTGGCAT CATGCCTCGA 
TTGATCATAA TCATGTCAGA TTCACATGGT AGGTTTGCTT CAAGACCAGA TGTCTCTGTA 
GATTGGACCA AAAGAGAAAT GTTACTTAAA AAAGAGGAAA AAGAAGCTGA ACCAATAACA 
CAAGAAAAGA TGGACTATAT AAAAGAAGCC CCAACTACAT ATATAATAGC AGAGGCTCCT 
GTGCAGATTA ATGTCACAAA ACCACCAGAT GCAAAGAAAG AAATACAAAT GGAAATGCCT 
GTACGAAAAC TTGAAGAAGG ACCAAAAGAA ATAAAAACAG AAATACCAGT GGAGATACCT 
GAAGAAAAAA CAAAAGATGT AAAAGAAGTT GTAGTAATAC CAGTGGAAAT GCCAGTGAAG 
AAAATTAAAC CGGAAGAAGA GATACCAGTG GAAATGCCAG TGAAAAAACT TAAAGAACCA 
GAAGAAATAC CAGTGGAAAT ACCAGTAGAG AAACCCAAAG AAGTATCAAA AGCAAAAGAA 
CAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTCAGAAGA CTGAACAAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAGGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963638.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819092.1 Xenopus tropicalis triadin (trdn), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OXa31772
Clone ID Related Accession (Same CDS sequence)	XM_012963638.2
Accession Version	XM_012963638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819092.1
CDS	391..5091
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963638.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963638.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963639.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819093.1 Xenopus tropicalis triadin (trdn), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OXa31773
Clone ID Related Accession (Same CDS sequence)	XM_012963639.2
Accession Version	XM_012963639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGATGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819093.1
CDS	391..5091
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPVKKPEAKPADVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963639.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963639.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGATGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963640.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819094.1 Xenopus tropicalis triadin (trdn), transcript variant X31, mRNA.

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CloneID	OXa31774
Clone ID Related Accession (Same CDS sequence)	XM_012963640.2
Accession Version	XM_012963640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819094.1
CDS	391..5091
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963640.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963640.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963642.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819096.1 Xenopus tropicalis triadin (trdn), transcript variant X28, mRNA.

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CloneID	OXa31775
Clone ID Related Accession (Same CDS sequence)	XM_012963642.2
Accession Version	XM_012963642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963642.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819096.1
CDS	391..5091
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963642.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963642.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963643.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819097.1 Xenopus tropicalis triadin (trdn), transcript variant X29, mRNA.

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CloneID	OXa31776
Clone ID Related Accession (Same CDS sequence)	XM_012963643.2
Accession Version	XM_012963643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819097.1
CDS	391..5091
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963643.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963643.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963644.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819098.1 Xenopus tropicalis triadin (trdn), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OXa31777
Clone ID Related Accession (Same CDS sequence)	XM_012963644.2
Accession Version	XM_012963644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819098.1
CDS	391..5091
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963644.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963644.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963645.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819099.1 Xenopus tropicalis triadin (trdn), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OXa31778
Clone ID Related Accession (Same CDS sequence)	XM_012963645.2
Accession Version	XM_012963645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGATGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGGTAAATAT 
TGTAGGCTCT GTAAAACTCT GTCTATAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819099.1
CDS	391..5043
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963645.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963645.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGATGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGGTAAATAT 
TGTAGGCTCT GTAAAACTCT GTCTATAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963646.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819100.1 Xenopus tropicalis triadin (trdn), transcript variant X34, mRNA.

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CloneID	OXa31779
Clone ID Related Accession (Same CDS sequence)	XM_012963646.2
Accession Version	XM_012963646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGGTAAATAT 
TGTAGGCTCT GTAAAACTCT GTCTATAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819100.1
CDS	391..5043
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963646.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963646.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGGTAAATAT 
TGTAGGCTCT GTAAAACTCT GTCTATAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963647.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819101.1 Xenopus tropicalis triadin (trdn), transcript variant X35, mRNA.

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CloneID	OXa31780
Clone ID Related Accession (Same CDS sequence)	XM_012963647.2
Accession Version	XM_012963647.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963647.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGGTAAATAT 
TGTAGGCTCT GTAAAACTCT GTCTATAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819101.1
CDS	391..5043
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963647.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963647.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGGTAAATAT 
TGTAGGCTCT GTAAAACTCT GTCTATAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963648.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819102.1 Xenopus tropicalis triadin (trdn), transcript variant X36, mRNA.

Price & Availability↑

CloneID	OXa31781
Clone ID Related Accession (Same CDS sequence)	XM_012963648.2
Accession Version	XM_012963648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGGTAAATAT 
TGTAGGCTCT GTAAAACTCT GTCTATAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819102.1
CDS	391..5043
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963648.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963648.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGGTAAATAT 
TGTAGGCTCT GTAAAACTCT GTCTATAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963649.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819103.1 Xenopus tropicalis triadin (trdn), transcript variant X37, mRNA.

Price & Availability↑

CloneID	OXa31782
Clone ID Related Accession (Same CDS sequence)	XM_012963649.2
Accession Version	XM_012963649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGGTAAATAT 
TGTAGGCTCT GTAAAACTCT GTCTATAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819103.1
CDS	391..5043
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963649.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963649.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGGTAAATAT 
TGTAGGCTCT GTAAAACTCT GTCTATAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963650.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819104.1 Xenopus tropicalis triadin (trdn), transcript variant X38, mRNA.

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CloneID	OXa31783
Clone ID Related Accession (Same CDS sequence)	XM_012963650.2
Accession Version	XM_012963650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X38
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGGTAAATAT 
TGTAGGCTCT GTAAAACTCT GTCTATAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819104.1
CDS	391..5043
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963650.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963650.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGGTAAATAT 
TGTAGGCTCT GTAAAACTCT GTCTATAAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963651.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819105.1 Xenopus tropicalis triadin (trdn), transcript variant X39, mRNA.

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CloneID	OXa31784
Clone ID Related Accession (Same CDS sequence)	XM_012963651.2
Accession Version	XM_012963651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X39
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGG AAGTTCACTT 
AACAAGTTCC GGAGTAATCC TGTCGGAATA ATACATGAAG CAGTGGATGA AACAACTGAT 
TGGATTTATG GAATATACAT TACTATATCT GACTTGATAT CCCTTGATGA AGAACGTAAA 
GGGCATGCTG AGCCTTCTGT AAAGAAAATA GGTGGAATAC AACCAGCTCT ACACAGAAAA 
GCAATACCAA AACTCAAGGC TGAAGAGGAA GATGAGCCAG TTAAGAAAAC CAGAGCAAAA 
GAAAAGGCTG AAACAAAAGA AAAAGTAGAG AAGAAAATTC TACCTAAAGC CTCACTAAAA 
GCAGAGAAGA AGGAGAAACC TGAAAAAATT ACCTTGACAA AATCTGAGAA GAAAGAAAAA 
TCTGCAATAA AACCAGAGAA AAAAGACAAG ACGGAAAAGG AAGCCTCAGT CAAAACTGAA 
AAGAAAGTAA AAGCTGCAAA ATTAGACAAA GTCAAAATTG ACAAGAAAGA AAAAGCTGAA 
AAACTTGTCA CACCCAAGTC TGAGAAGAAG GAAAAAACTG AAAAAGTAAC CCCATCCAAA 
CTTGAGAAAA AAGTAAAAGC TGAACAGATA GCTCCCACAA AACTAGAGAA AAAGGAAAAA 
ACTGAAAAGG TAGCAACCAC AAAATCAGAA AAGAAAGAAA AAGCTGAAAA GTTAACCCCA 
GTCAAAGTAG AGAAGACATT AAAACTTGAA AAGGTAGCAC CAGTTAAAGC TGAGAAAAAG 
GAAAAAGCTG AAAAAGTAGT CCCTGTTAAA GTTCAGAAAA AGGAAAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGAAAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGACAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGAAAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGACAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTTCCAGTTA AAGCAGAGAA GAAAGGCAAA GCTGAAAAAG TAGCCCCTGT CAAACCCCAG 
AAGGAGAAAG CTGAAAAAGT AACCTCGGTC AAAGCTGAAA AGAAGGAAAA AGCTGAAAAA 
ATTGCCCCAG TAAAAGCCGA GAAGAAGGAA AAAGTTGAAA AGATAGCCAC AAGTAAAGCC 
GAGAAGAAGG TTGAAAAGTT AGCCCCAGTC AAACCTGAGA AAAAGGAAAA GGCTAAAAAA 
GTAGCCCCAG TCAAATCTGA GAAGAAGGAA AAGACTGAAA AGTTAACCCA AGACACAACC 
GAGAAGGTAA AAGCTGAAAA GGTTGCTCCA ACCAAATTGG AGAAGAAACA AAAAGCTGAA 
ACCAGGGCCC CAGTCAAAAC TGAGAAGAAG GACAAAGCAG AAAAGGAAAT CATACCACAA 
GAAGACAAAA AAGTCAAGGT TTCTCAGGTG GAAGAAAAGA CTAAGGATGT CAAAGCTAAA 
ACATCAGAGA GAAAAGTAGC AAAAGACTCA AAGAAAGACG CTAAAGAGGA AGAAGAAATC 
AAGACAAAGC CACATACTAA AGTGGAAACA CTGGATGCAG CAGAAACAAT GGGGGAAATA 
AAAGATCAGT ATAATTTCTG CCGTTATGTG GTTGACCTGT ATTCTCATGG AGATGCTTCT 
ACAGAAGAAA AATCAAAGGA AAAACCACAA GAGAAGAAAG AAGTTCTCCC ACAAGAAAAA 
CCAGGAGAAT TGAAAATATC ATTACCAGAA AAGAAAGATA TTCCTGAAAC AAAGGAAACA 
GTCTTAGAGG AGGTGACAAT TCATGAAGCT GAAGACGAAT GCCCAAAGGA AGATTATGAA 
CAAGTTACAC CTCAAGCTAC TGAAAAAGAA CCCCCAAAAG TCATGCAAGA TCATTTAACA 
GTTCATAAAG ATACAGAAGA AAGTCCAAAA AAAGAGCAAG AACCCATGAC ACCTCAGATA 
GTAAAAGATG GACCACCAAA ACCAGTTGAA GCTGAAGTAA AAACAGAAGA GAAGAAAGAA 
GGAAGAAGCC AACAGCCTGC ATTTACCGGT GTATCTACAG ATTTTGGCAT CATGCCTCGA 
TTGATCATAA TCATGTCAGA TTCACATGGT AGGTTTGCTT CAAGACCAGA TGTCTCTGTA 
GATTGGACCA AAAGAGAAAT GTTACTTAAA AAAGAGGAAA AAGAAGCTGA ACCAATAACA 
CAAGAAAAGA TGGACTATAT AAAAGAAGCC CCAACTACAT ATATAATAGC AGAGGCTCCT 
GTGCAGATTA ATGTCACAAA ACCACCAGAT GCAAAGAAAG AAATACAAAT GGAAATGCCT 
GTACGAAAAC TTGAAGAAGG ACCAAAAGAA ATAAAAACAG AAATACCAGT GGAGATACCT 
GAAGAAAAAA CAAAAGATGT AAAAGAAGTT GTAGTAATAC CAGTGGAAAT GCCAGTGAAG 
AAAATTAAAC CGGAAGAAGA GATACCAGTG GAAATGCCAG TGAAAAAACT TAAAGAACCA 
GAAGAAATAC CAGTGGAAAT ACCAGTAGAG AAACCCAAAG AAGTATCAAA AGCAAAAGAA 
CAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTCAGAAGA CTGAACAAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAGGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819105.1
CDS	391..5031
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963651.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963651.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGG AAGTTCACTT 
AACAAGTTCC GGAGTAATCC TGTCGGAATA ATACATGAAG CAGTGGATGA AACAACTGAT 
TGGATTTATG GAATATACAT TACTATATCT GACTTGATAT CCCTTGATGA AGAACGTAAA 
GGGCATGCTG AGCCTTCTGT AAAGAAAATA GGTGGAATAC AACCAGCTCT ACACAGAAAA 
GCAATACCAA AACTCAAGGC TGAAGAGGAA GATGAGCCAG TTAAGAAAAC CAGAGCAAAA 
GAAAAGGCTG AAACAAAAGA AAAAGTAGAG AAGAAAATTC TACCTAAAGC CTCACTAAAA 
GCAGAGAAGA AGGAGAAACC TGAAAAAATT ACCTTGACAA AATCTGAGAA GAAAGAAAAA 
TCTGCAATAA AACCAGAGAA AAAAGACAAG ACGGAAAAGG AAGCCTCAGT CAAAACTGAA 
AAGAAAGTAA AAGCTGCAAA ATTAGACAAA GTCAAAATTG ACAAGAAAGA AAAAGCTGAA 
AAACTTGTCA CACCCAAGTC TGAGAAGAAG GAAAAAACTG AAAAAGTAAC CCCATCCAAA 
CTTGAGAAAA AAGTAAAAGC TGAACAGATA GCTCCCACAA AACTAGAGAA AAAGGAAAAA 
ACTGAAAAGG TAGCAACCAC AAAATCAGAA AAGAAAGAAA AAGCTGAAAA GTTAACCCCA 
GTCAAAGTAG AGAAGACATT AAAACTTGAA AAGGTAGCAC CAGTTAAAGC TGAGAAAAAG 
GAAAAAGCTG AAAAAGTAGT CCCTGTTAAA GTTCAGAAAA AGGAAAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGAAAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGACAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGAAAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTCCCTGTTA AAGATGAGAA AAAGGACAAA GCTGAAAAAA AGCAGAAAGC TGAAAAGGTA 
GTTCCAGTTA AAGCAGAGAA GAAAGGCAAA GCTGAAAAAG TAGCCCCTGT CAAACCCCAG 
AAGGAGAAAG CTGAAAAAGT AACCTCGGTC AAAGCTGAAA AGAAGGAAAA AGCTGAAAAA 
ATTGCCCCAG TAAAAGCCGA GAAGAAGGAA AAAGTTGAAA AGATAGCCAC AAGTAAAGCC 
GAGAAGAAGG TTGAAAAGTT AGCCCCAGTC AAACCTGAGA AAAAGGAAAA GGCTAAAAAA 
GTAGCCCCAG TCAAATCTGA GAAGAAGGAA AAGACTGAAA AGTTAACCCA AGACACAACC 
GAGAAGGTAA AAGCTGAAAA GGTTGCTCCA ACCAAATTGG AGAAGAAACA AAAAGCTGAA 
ACCAGGGCCC CAGTCAAAAC TGAGAAGAAG GACAAAGCAG AAAAGGAAAT CATACCACAA 
GAAGACAAAA AAGTCAAGGT TTCTCAGGTG GAAGAAAAGA CTAAGGATGT CAAAGCTAAA 
ACATCAGAGA GAAAAGTAGC AAAAGACTCA AAGAAAGACG CTAAAGAGGA AGAAGAAATC 
AAGACAAAGC CACATACTAA AGTGGAAACA CTGGATGCAG CAGAAACAAT GGGGGAAATA 
AAAGATCAGT ATAATTTCTG CCGTTATGTG GTTGACCTGT ATTCTCATGG AGATGCTTCT 
ACAGAAGAAA AATCAAAGGA AAAACCACAA GAGAAGAAAG AAGTTCTCCC ACAAGAAAAA 
CCAGGAGAAT TGAAAATATC ATTACCAGAA AAGAAAGATA TTCCTGAAAC AAAGGAAACA 
GTCTTAGAGG AGGTGACAAT TCATGAAGCT GAAGACGAAT GCCCAAAGGA AGATTATGAA 
CAAGTTACAC CTCAAGCTAC TGAAAAAGAA CCCCCAAAAG TCATGCAAGA TCATTTAACA 
GTTCATAAAG ATACAGAAGA AAGTCCAAAA AAAGAGCAAG AACCCATGAC ACCTCAGATA 
GTAAAAGATG GACCACCAAA ACCAGTTGAA GCTGAAGTAA AAACAGAAGA GAAGAAAGAA 
GGAAGAAGCC AACAGCCTGC ATTTACCGGT GTATCTACAG ATTTTGGCAT CATGCCTCGA 
TTGATCATAA TCATGTCAGA TTCACATGGT AGGTTTGCTT CAAGACCAGA TGTCTCTGTA 
GATTGGACCA AAAGAGAAAT GTTACTTAAA AAAGAGGAAA AAGAAGCTGA ACCAATAACA 
CAAGAAAAGA TGGACTATAT AAAAGAAGCC CCAACTACAT ATATAATAGC AGAGGCTCCT 
GTGCAGATTA ATGTCACAAA ACCACCAGAT GCAAAGAAAG AAATACAAAT GGAAATGCCT 
GTACGAAAAC TTGAAGAAGG ACCAAAAGAA ATAAAAACAG AAATACCAGT GGAGATACCT 
GAAGAAAAAA CAAAAGATGT AAAAGAAGTT GTAGTAATAC CAGTGGAAAT GCCAGTGAAG 
AAAATTAAAC CGGAAGAAGA GATACCAGTG GAAATGCCAG TGAAAAAACT TAAAGAACCA 
GAAGAAATAC CAGTGGAAAT ACCAGTAGAG AAACCCAAAG AAGTATCAAA AGCAAAAGAA 
CAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
ATTCAGAAGA CTGAACAAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAGGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGATGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG GTAAATATTG TAGGCTCTGT 
AAAACTCTGT CTATAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963653.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819107.1 Xenopus tropicalis triadin (trdn), transcript variant X41, mRNA.

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CloneID	OXa31785
Clone ID Related Accession (Same CDS sequence)	XM_012963653.2 , XM_012963654.2
Accession Version	XM_012963653.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X41
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAT GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819107.1
CDS	391..4995
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963653.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963653.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAT GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963654.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819108.1 Xenopus tropicalis triadin (trdn), transcript variant X40, mRNA.

Price & Availability↑

CloneID	OXa31785
Clone ID Related Accession (Same CDS sequence)	XM_012963653.2 , XM_012963654.2
Accession Version	XM_012963654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAT GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819108.1
CDS	391..4995
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963654.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963654.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAT GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963655.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819109.1 Xenopus tropicalis triadin (trdn), transcript variant X43, mRNA.

Price & Availability↑

CloneID	OXa31786
Clone ID Related Accession (Same CDS sequence)	XM_012963655.2
Accession Version	XM_012963655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X43
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGATGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAT GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819109.1
CDS	391..4995
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPVKKPEAKPADVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963655.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X43, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963655.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGATGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAT GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963656.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819110.1 Xenopus tropicalis triadin (trdn), transcript variant X42, mRNA.

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CloneID	OXa31787
Clone ID Related Accession (Same CDS sequence)	XM_012963656.2
Accession Version	XM_012963656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X42
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAT GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819110.1
CDS	391..4995
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963656.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X42, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963656.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAT GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963657.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819111.1 Xenopus tropicalis triadin (trdn), transcript variant X44, mRNA.

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CloneID	OXa31788
Clone ID Related Accession (Same CDS sequence)	XM_012963657.2
Accession Version	XM_012963657.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X44
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAT GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819111.1
CDS	391..4995
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963657.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X44, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963657.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAT GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963658.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819112.1 Xenopus tropicalis triadin (trdn), transcript variant X45, mRNA.

Price & Availability↑

CloneID	OXa31789
Clone ID Related Accession (Same CDS sequence)	XM_012963658.2
Accession Version	XM_012963658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X45
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819112.1
CDS	391..4995
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963658.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X45, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963658.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG AAGGCTGAAC CAGAAGTAAA GGACATTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCT 
GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA GTAAAGAAAG AGGTGCCCAT TAAGAAGACT 
GAACCAGAAG TGAAGGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_018094232.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949721.1 Xenopus tropicalis triadin (trdn), transcript variant X46, mRNA.

Price & Availability↑

CloneID	OXa31790
Clone ID Related Accession (Same CDS sequence)	XM_018094232.1
Accession Version	XM_018094232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X46
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949721.1
CDS	391..4995
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_018094232.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X46, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094232.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCATTC AGAAGACTGA ACAAGAAGTG 
AAGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAGGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGGTAAAT ATTGTAGGCT CTGTAAAACT CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_012963660.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819114.1 Xenopus tropicalis triadin (trdn), transcript variant X47, mRNA.

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CloneID	OXa31791
Clone ID Related Accession (Same CDS sequence)	XM_012963660.2
Accession Version	XM_012963660.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X47
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963660.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGGTAA ATATTGTAGG CTCTGTAAAA CTCTGTCTAT AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819114.1
CDS	391..4947
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_012963660.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X47, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963660.2

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGAACC CATGACACCT CAGATAGTAA AAGATGGACC ACCAAAACCA 
GTTGAAGCTG AAGTAAAAAC AGAAGAGAAG AAAGAAGGAA GAAGCCAACA GCCTGCATTT 
ACCGGTGTAT CTACAGATTT TGGCATCATG CCTCGATTGA TCATAATCAT GTCAGATTCA 
CATGGTAGGT TTGCTTCAAG ACCAGATGTC TCTGTAGATT GGACCAAAAG AGAAATGTTA 
CTTAAAAAAG AGGAAAAAGA AGCTGAACCA ATAACACAAG AAAAGATGGA CTATATAAAA 
GAAGCCCCAA CTACATATAT AATAGCAGAG GCTCCTGTGC AGATTAATGT CACAAAACCA 
CCAGATGCAA AGAAAGAAAT ACAAATGGAA ATGCCTGTAC GAAAACTTGA AGAAGGACCA 
AAAGAAATAA AAACAGAAAT ACCAGTGGAG ATACCTGAAG AAAAAACAAA AGATGTAAAA 
GAAGTTGTAG TAATACCAGT GGAAATGCCA GTGAAGAAAA TTAAACCGGA AGAAGAGATA 
CCAGTGGAAA TGCCAGTGAA AAAACTTAAA GAACCAGAAG AAATACCAGT GGAAATACCA 
GTAGAGAAAC CCAAAGAAGT ATCAAAAGCA AAAGAACAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAA 
AAAGAGGAAC CAGTTAAGAA GGCTGAACCA GAAGTAAAGG ACATTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCTGT AAAGAAACCT 
GAAGCAAAAC CTGCAGAAGT AAAGAAAGAG GTGCCCATTA AGAAGACTGA ACCAGAAGTG 
ATGGAAGTTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CCTTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAAAA AGAGGAACCA 
GTTAAGAAGG CTGAACCAGA AGTAAAGGAC ATTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCTGTAA AGAAACCTGA AGCAAAACCT 
GCAGAAGTAA AGAAAGAGGT GCCCATTAAG AAGACTGAAC CAGAAGTGAA GGAAGTTAAG 
AAAGAGGTGC CATTAAAGAA GCCCGAACCA AAACCTGCAG AAGTTAAGAA AGAGGTGCCC 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAAAAAG AGGAACCAGT TAAGAAGGCT 
GAACCAGAAG TAAAGGACAT TAAGAAAGAG GTGCCATTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG AAACCTGAAG CAAAACCTGC AGAAGTAAAG 
AAAGAGGTGC CCATTAAGAA GACTGAACCA GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA 
TTAAAGAAGC CCGAACCAAA ACCTGCAGAA GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAAAAAGAG GAACCAGTTA AGAAGGCTGA ACCAGAAGTA 
AAGGACATTA AGAAAGAGGT GCCATTAAAG AAGCCCGAAC CAAAACCTGC AGAAGTTAAG 
AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA AAACCTGCAG AAGTAAAGAA AGAGGTGCCC 
ATTAAGAAGA CTGAACCAGA AGTGAAGGAA GTTAAGAAAG AGGTGCCATT AAAGAAGCCC 
GAACCAAAAC CTGCAGAAGT TAAGAAAGAG GTGCCCTTAA AGAAGCCCGA ACCAAAACCT 
GCAGAAGTTA AAAAAGGTAA ATATTGTAGG CTCTGTAAAA CTCTGTCTAT AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_018094229.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949718.1 Xenopus tropicalis triadin (trdn), transcript variant X21, mRNA.

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CloneID	OXa44647
Clone ID Related Accession (Same CDS sequence)	XM_018094229.1
Accession Version	XM_018094229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGCTGA AGTAAAAACA GAAGAGAAGA AAGAAGGAAG AAGCCAACAG 
CCTGCATTTA CCGGTGTATC TACAGATTTT GGCATCATGC CTCGATTGAT CATAATCATG 
TCAGATTCAC ATGGTAGGTT TGCTTCAAGA CCAGATGTCT CTGTAGATTG GACCAAAAGA 
GAAATGTTAC TTAAAAAAGA GGAAAAAGAA GCTGAACCAA TAACACAAGA AAAGATGGAC 
TATATAAAAG AAGCCCCAAC TACATATATA ATAGCAGAGG CTCCTGTGCA GATTAATGTC 
ACAAAACCAC CAGATGCAAA GAAAGAAATA CAAATGGAAA TGCCTGTACG AAAACTTGAA 
GAAGGACCAA AAGAAATAAA AACAGAAATA CCAGTGGAGA TACCTGAAGA AAAAACAAAA 
GATGTAAAAG AAGTTGTAGT AATACCAGTG GAAATGCCAG TGAAGAAAAT TAAACCGGAA 
GAAGAGATAC CAGTGGAAAT GCCAGTGAAA AAACTTAAAG AACCAGAAGA AATACCAGTG 
GAAATACCAG TAGAGAAACC CAAAGAAGTA TCAAAAGCAA AAGAACAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCATTCA GAAGACTGAA 
CAAGAAGTGA AGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAGGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCCTTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAAA AAGAGGAACC AGTTAAGAAG GCTGAACCAG AAGTAAAGGA CATTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCTGTA 
AAGAAACCTG AAGCAAAACC TGCAGAAGTA AAGAAAGAGG TGCCCATTAA GAAGACTGAA 
CCAGAAGTGA TGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC CTTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAAAAA 
GAGGAACCAG TTAAGAAGGC TGAACCAGAA GTAAAGGACA TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCTGTAAA GAAACCTGAA 
GCAAAACCTG CAGAAGTAAA GAAAGAGGTG CCCATTAAGA AGACTGAACC AGAAGTGAAG 
GAAGTTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAAAAAGA GGAACCAGTT 
AAGAAGGCTG AACCAGAAGT AAAGGACATT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCTGTAAAGA AACCTGAAGC AAAACCTGCA 
GAAGTAAAGA AAGAGGTGCC CATTAAGAAG ACTGAACCAG AAGTGAAGGA AGTTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGGTAAA TATTGTAGGC TCTGTAAAAC TCTGTCTATA 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949718.1
CDS	391..5136
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYEQVTPQATEKEPPKVMQDHLTVHKDTEESPKKEQAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_018094229.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094229.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGAACAAG TTACACCTCA AGCTACTGAA 
AAAGAACCCC CAAAAGTCAT GCAAGATCAT TTAACAGTTC ATAAAGATAC AGAAGAAAGT 
CCAAAAAAAG AGCAAGCTGA AGTAAAAACA GAAGAGAAGA AAGAAGGAAG AAGCCAACAG 
CCTGCATTTA CCGGTGTATC TACAGATTTT GGCATCATGC CTCGATTGAT CATAATCATG 
TCAGATTCAC ATGGTAGGTT TGCTTCAAGA CCAGATGTCT CTGTAGATTG GACCAAAAGA 
GAAATGTTAC TTAAAAAAGA GGAAAAAGAA GCTGAACCAA TAACACAAGA AAAGATGGAC 
TATATAAAAG AAGCCCCAAC TACATATATA ATAGCAGAGG CTCCTGTGCA GATTAATGTC 
ACAAAACCAC CAGATGCAAA GAAAGAAATA CAAATGGAAA TGCCTGTACG AAAACTTGAA 
GAAGGACCAA AAGAAATAAA AACAGAAATA CCAGTGGAGA TACCTGAAGA AAAAACAAAA 
GATGTAAAAG AAGTTGTAGT AATACCAGTG GAAATGCCAG TGAAGAAAAT TAAACCGGAA 
GAAGAGATAC CAGTGGAAAT GCCAGTGAAA AAACTTAAAG AACCAGAAGA AATACCAGTG 
GAAATACCAG TAGAGAAACC CAAAGAAGTA TCAAAAGCAA AAGAACAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCATTCA GAAGACTGAA 
CAAGAAGTGA AGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAGGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCCTTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAAA AAGAGGAACC AGTTAAGAAG GCTGAACCAG AAGTAAAGGA CATTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCTGTA 
AAGAAACCTG AAGCAAAACC TGCAGAAGTA AAGAAAGAGG TGCCCATTAA GAAGACTGAA 
CCAGAAGTGA TGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC CTTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAAAAA 
GAGGAACCAG TTAAGAAGGC TGAACCAGAA GTAAAGGACA TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCTGTAAA GAAACCTGAA 
GCAAAACCTG CAGAAGTAAA GAAAGAGGTG CCCATTAAGA AGACTGAACC AGAAGTGAAG 
GAAGTTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAAAAAGA GGAACCAGTT 
AAGAAGGCTG AACCAGAAGT AAAGGACATT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCTGTAAAGA AACCTGAAGC AAAACCTGCA 
GAAGTAAAGA AAGAGGTGCC CATTAAGAAG ACTGAACCAG AAGTGAAGGA AGTTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGGTAAA TATTGTAGGC TCTGTAAAAC TCTGTCTATA 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_018094230.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949719.1 Xenopus tropicalis triadin (trdn), transcript variant X22, mRNA.

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CloneID	OXa44648
Clone ID Related Accession (Same CDS sequence)	XM_018094230.1
Accession Version	XM_018094230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGATCATT TAACAGTTCA TAAAGATACA 
GAAGAAAGTC CAAAAAAAGA GCAAGAACCC ATGACACCTC AGATAGTAAA AGATGGACCA 
CCAAAACCAG TTGAAGCTGA AGTAAAAACA GAAGAGAAGA AAGAAGGAAG AAGCCAACAG 
CCTGCATTTA CCGGTGTATC TACAGATTTT GGCATCATGC CTCGATTGAT CATAATCATG 
TCAGATTCAC ATGGTAGGTT TGCTTCAAGA CCAGATGTCT CTGTAGATTG GACCAAAAGA 
GAAATGTTAC TTAAAAAAGA GGAAAAAGAA GCTGAACCAA TAACACAAGA AAAGATGGAC 
TATATAAAAG AAGCCCCAAC TACATATATA ATAGCAGAGG CTCCTGTGCA GATTAATGTC 
ACAAAACCAC CAGATGCAAA GAAAGAAATA CAAATGGAAA TGCCTGTACG AAAACTTGAA 
GAAGGACCAA AAGAAATAAA AACAGAAATA CCAGTGGAGA TACCTGAAGA AAAAACAAAA 
GATGTAAAAG AAGTTGTAGT AATACCAGTG GAAATGCCAG TGAAGAAAAT TAAACCGGAA 
GAAGAGATAC CAGTGGAAAT GCCAGTGAAA AAACTTAAAG AACCAGAAGA AATACCAGTG 
GAAATACCAG TAGAGAAACC CAAAGAAGTA TCAAAAGCAA AAGAACAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCATTCA GAAGACTGAA 
CAAGAAGTGA AGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAGGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCCTTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAAA AAGAGGAACC AGTTAAGAAG GCTGAACCAG AAGTAAAGGA CATTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCTGTA 
AAGAAACCTG AAGCAAAACC TGCAGAAGTA AAGAAAGAGG TGCCCATTAA GAAGACTGAA 
CCAGAAGTGA TGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC CTTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAAAAA 
GAGGAACCAG TTAAGAAGGC TGAACCAGAA GTAAAGGACA TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCTGTAAA GAAACCTGAA 
GCAAAACCTG CAGAAGTAAA GAAAGAGGTG CCCATTAAGA AGACTGAACC AGAAGTGAAG 
GAAGTTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAAAAAGA GGAACCAGTT 
AAGAAGGCTG AACCAGAAGT AAAGGACATT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCTGTAAAGA AACCTGAAGC AAAACCTGCA 
GAAGTAAAGA AAGAGGTGCC CATTAAGAAG ACTGAACCAG AAGTGAAGGA AGTTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGGTAAA TATTGTAGGC TCTGTAAAAC TCTGTCTATA 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949719.1
CDS	391..5136
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYDHLTVHKDTEESPKKEQEPMTPQIVKDGPPKPVEAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_018094230.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094230.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGATCATT TAACAGTTCA TAAAGATACA 
GAAGAAAGTC CAAAAAAAGA GCAAGAACCC ATGACACCTC AGATAGTAAA AGATGGACCA 
CCAAAACCAG TTGAAGCTGA AGTAAAAACA GAAGAGAAGA AAGAAGGAAG AAGCCAACAG 
CCTGCATTTA CCGGTGTATC TACAGATTTT GGCATCATGC CTCGATTGAT CATAATCATG 
TCAGATTCAC ATGGTAGGTT TGCTTCAAGA CCAGATGTCT CTGTAGATTG GACCAAAAGA 
GAAATGTTAC TTAAAAAAGA GGAAAAAGAA GCTGAACCAA TAACACAAGA AAAGATGGAC 
TATATAAAAG AAGCCCCAAC TACATATATA ATAGCAGAGG CTCCTGTGCA GATTAATGTC 
ACAAAACCAC CAGATGCAAA GAAAGAAATA CAAATGGAAA TGCCTGTACG AAAACTTGAA 
GAAGGACCAA AAGAAATAAA AACAGAAATA CCAGTGGAGA TACCTGAAGA AAAAACAAAA 
GATGTAAAAG AAGTTGTAGT AATACCAGTG GAAATGCCAG TGAAGAAAAT TAAACCGGAA 
GAAGAGATAC CAGTGGAAAT GCCAGTGAAA AAACTTAAAG AACCAGAAGA AATACCAGTG 
GAAATACCAG TAGAGAAACC CAAAGAAGTA TCAAAAGCAA AAGAACAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCATTCA GAAGACTGAA 
CAAGAAGTGA AGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAGGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCCTTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAAA AAGAGGAACC AGTTAAGAAG GCTGAACCAG AAGTAAAGGA CATTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCTGTA 
AAGAAACCTG AAGCAAAACC TGCAGAAGTA AAGAAAGAGG TGCCCATTAA GAAGACTGAA 
CCAGAAGTGA TGGAAGTTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC CTTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAAAAA 
GAGGAACCAG TTAAGAAGGC TGAACCAGAA GTAAAGGACA TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCTGTAAA GAAACCTGAA 
GCAAAACCTG CAGAAGTAAA GAAAGAGGTG CCCATTAAGA AGACTGAACC AGAAGTGAAG 
GAAGTTAAGA AAGAGGTGCC ATTAAAGAAG CCCGAACCAA AACCTGCAGA AGTTAAGAAA 
GAGGTGCCCT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAAAAAGA GGAACCAGTT 
AAGAAGGCTG AACCAGAAGT AAAGGACATT AAGAAAGAGG TGCCATTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA GAAAGAGGTG CCTGTAAAGA AACCTGAAGC AAAACCTGCA 
GAAGTAAAGA AAGAGGTGCC CATTAAGAAG ACTGAACCAG AAGTGAAGGA AGTTAAGAAA 
GAGGTGCCAT TAAAGAAGCC CGAACCAAAA CCTGCAGAAG TTAAGAAAGA GGTGCCCTTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAAAAAGAGG AACCAGTTAA GAAGGCTGAA 
CCAGAAGTAA AGGACATTAA GAAAGAGGTG CCATTAAAGA AGCCCGAACC AAAACCTGCA 
GAAGTTAAGA AAGAGGTGCC TGTAAAGAAA CCTGAAGCAA AACCTGCAGA AGTAAAGAAA 
GAGGTGCCCA TTAAGAAGAC TGAACCAGAA GTGAAGGAAG TTAAGAAAGA GGTGCCATTA 
AAGAAGCCCG AACCAAAACC TGCAGAAGTT AAGAAAGAGG TGCCCTTAAA GAAGCCCGAA 
CCAAAACCTG CAGAAGTTAA AAAAGGTAAA TATTGTAGGC TCTGTAAAAC TCTGTCTATA 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trdn ( XM_018094231.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949720.1 Xenopus tropicalis triadin (trdn), transcript variant X32, mRNA.

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CloneID	OXa44649
Clone ID Related Accession (Same CDS sequence)	XM_018094231.1
Accession Version	XM_018094231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	triadin isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGATCATT TAACAGTTCA TAAAGATACA 
GAAGAAAGTC CAAAAAAAGA GCAAGCTGAA GTAAAAACAG AAGAGAAGAA AGAAGGAAGA 
AGCCAACAGC CTGCATTTAC CGGTGTATCT ACAGATTTTG GCATCATGCC TCGATTGATC 
ATAATCATGT CAGATTCACA TGGTAGGTTT GCTTCAAGAC CAGATGTCTC TGTAGATTGG 
ACCAAAAGAG AAATGTTACT TAAAAAAGAG GAAAAAGAAG CTGAACCAAT AACACAAGAA 
AAGATGGACT ATATAAAAGA AGCCCCAACT ACATATATAA TAGCAGAGGC TCCTGTGCAG 
ATTAATGTCA CAAAACCACC AGATGCAAAG AAAGAAATAC AAATGGAAAT GCCTGTACGA 
AAACTTGAAG AAGGACCAAA AGAAATAAAA ACAGAAATAC CAGTGGAGAT ACCTGAAGAA 
AAAACAAAAG ATGTAAAAGA AGTTGTAGTA ATACCAGTGG AAATGCCAGT GAAGAAAATT 
AAACCGGAAG AAGAGATACC AGTGGAAATG CCAGTGAAAA AACTTAAAGA ACCAGAAGAA 
ATACCAGTGG AAATACCAGT AGAGAAACCC AAAGAAGTAT CAAAAGCAAA AGAACAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCATTCAG 
AAGACTGAAC AAGAAGTGAA GGAAGTTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AGGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCTGTAA AGAAACCTGA AGCAAAACCT GCAGAAGTAA AGAAAGAGGT GCCCATTAAG 
AAGACTGAAC CAGAAGTGAT GGAAGTTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCC TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAAAAAG AGGAACCAGT TAAGAAGGCT GAACCAGAAG TAAAGGACAT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG 
AAACCTGAAG CAAAACCTGC AGAAGTAAAG AAAGAGGTGC CCATTAAGAA GACTGAACCA 
GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAAAAAGAG 
GAACCAGTTA AGAAGGCTGA ACCAGAAGTA AAGGACATTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA 
AAACCTGCAG AAGTAAAGAA AGAGGTGCCC ATTAAGAAGA CTGAACCAGA AGTGAAGGAA 
GTTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGGTAAAT ATTGTAGGCT CTGTAAAACT 
CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949720.1
CDS	391..5085
Translation	MTENSVEGRTSSTITTVLEGKNGSIHKPSAQVKSVSHDFKTTFKSPVAWLLVGALIVTWSAVIIVVFDLVDYRSLGDTITQYCDDPCLPPGPHQPADGKVLMEAESFLGSSLNKFRSNPVGIIHEAVDETTDWIYGIYITISDLISLDEERKGHAEPSVKKIGGIQPALHRKVIHKEKPEVKENILKKASAKAIPKLKAEEEDEPVKKTRAKEKAETKEKVEKKILPKASLKAEKKEKPEKITLTKSEKKEKSAIKPEKKDKTEKEASVKTEKKVKAAKLDKVKIDKKEKAEKLVTPKSEKKEKTEKVTPSKLEKKVKAEQIAPTKLEKKEKTEKVATTKSEKKEKAEKLTPVKVEKTLKLEKVAPVKAEKKEKAEKVVPVKVQKKEKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKDEKKEKAEKKQKAEKVVPVKDEKKDKAEKKQKAEKVVPVKAEKKGKAEKVAPVKPQKEKAEKVTSVKAEKKEKAEKIAPVKAEKKEKVEKIATSKAEKKVEKLAPVKPEKKEKAKKVAPVKSEKKEKTEKLTQDTTEKVKAEKVAPTKLEKKQKAETRAPVKTEKKDKAEKEIIPQEDKKVKVSQVEEKTKDVKAKTSERKVAKDSKKDAKEEEEIKTKPHTKVETLDAAETMGEIKDQYNFCRYVVDLYSHGDASTEEKSKEKPQEKKEVLPQEKPGELKISLPEKKDIPETKETVLEEVTIHEAEDECPKEDYDHLTVHKDTEESPKKEQAEVKTEEKKEGRSQQPAFTGVSTDFGIMPRLIIIMSDSHGRFASRPDVSVDWTKREMLLKKEEKEAEPITQEKMDYIKEAPTTYIIAEAPVQINVTKPPDAKKEIQMEMPVRKLEEGPKEIKTEIPVEIPEEKTKDVKEVVVIPVEMPVKKIKPEEEIPVEMPVKKLKEPEEIPVEIPVEKPKEVSKAKEQEVPLKKPEPKPAEVKKEVPIQKTEQEVKEVKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVMEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKEEPVKKAEPEVKDIKKEVPLKKPEPKPAEVKKEVPVKKPEAKPAEVKKEVPIKKTEPEVKEVKKEVPLKKPEPKPAEVKKEVPLKKPEPKPAEVKKGKYCRLCKTLSIR

Target ORF information:

RefSeq Version	XM_018094231.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis triadin (trdn), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094231.1

ORF Insert Sequence:

ATGACGGAGA ACTCTGTTGA AGGTCGTACA TCCTCTACCA TAACAACTGT TTTAGAAGGC 
AAGAATGGCT CCATCCACAA ACCATCTGCA CAAGTAAAGT CTGTCTCACA TGATTTCAAA 
ACTACTTTCA AGTCTCCAGT AGCATGGCTT CTAGTTGGAG CACTTATAGT TACTTGGTCA 
GCCGTGATCA TTGTTGTATT CGATTTGGTG GATTACAGAT CATTAGGAGA CACTATTACA 
CAGTACTGTG ACGATCCCTG TTTACCACCT GGACCTCATC AGCCTGCTGA TGGCAAGGTT 
TTGATGGAAG CTGAGAGCTT TCTAGGAAGT TCACTTAACA AGTTCCGGAG TAATCCTGTC 
GGAATAATAC ATGAAGCAGT GGATGAAACA ACTGATTGGA TTTATGGAAT ATACATTACT 
ATATCTGACT TGATATCCCT TGATGAAGAA CGTAAAGGGC ATGCTGAGCC TTCTGTAAAG 
AAAATAGGTG GAATACAACC AGCTCTACAC AGAAAAGTAA TTCATAAAGA GAAACCTGAA 
GTAAAAGAGA ACATTTTGAA AAAGGCCTCA GCTAAAGCAA TACCAAAACT CAAGGCTGAA 
GAGGAAGATG AGCCAGTTAA GAAAACCAGA GCAAAAGAAA AGGCTGAAAC AAAAGAAAAA 
GTAGAGAAGA AAATTCTACC TAAAGCCTCA CTAAAAGCAG AGAAGAAGGA GAAACCTGAA 
AAAATTACCT TGACAAAATC TGAGAAGAAA GAAAAATCTG CAATAAAACC AGAGAAAAAA 
GACAAGACGG AAAAGGAAGC CTCAGTCAAA ACTGAAAAGA AAGTAAAAGC TGCAAAATTA 
GACAAAGTCA AAATTGACAA GAAAGAAAAA GCTGAAAAAC TTGTCACACC CAAGTCTGAG 
AAGAAGGAAA AAACTGAAAA AGTAACCCCA TCCAAACTTG AGAAAAAAGT AAAAGCTGAA 
CAGATAGCTC CCACAAAACT AGAGAAAAAG GAAAAAACTG AAAAGGTAGC AACCACAAAA 
TCAGAAAAGA AAGAAAAAGC TGAAAAGTTA ACCCCAGTCA AAGTAGAGAA GACATTAAAA 
CTTGAAAAGG TAGCACCAGT TAAAGCTGAG AAAAAGGAAA AAGCTGAAAA AGTAGTCCCT 
GTTAAAGTTC AGAAAAAGGA AAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GAAAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTCC CTGTTAAAGA TGAGAAAAAG 
GACAAAGCTG AAAAAAAGCA GAAAGCTGAA AAGGTAGTTC CAGTTAAAGC AGAGAAGAAA 
GGCAAAGCTG AAAAAGTAGC CCCTGTCAAA CCCCAGAAGG AGAAAGCTGA AAAAGTAACC 
TCGGTCAAAG CTGAAAAGAA GGAAAAAGCT GAAAAAATTG CCCCAGTAAA AGCCGAGAAG 
AAGGAAAAAG TTGAAAAGAT AGCCACAAGT AAAGCCGAGA AGAAGGTTGA AAAGTTAGCC 
CCAGTCAAAC CTGAGAAAAA GGAAAAGGCT AAAAAAGTAG CCCCAGTCAA ATCTGAGAAG 
AAGGAAAAGA CTGAAAAGTT AACCCAAGAC ACAACCGAGA AGGTAAAAGC TGAAAAGGTT 
GCTCCAACCA AATTGGAGAA GAAACAAAAA GCTGAAACCA GGGCCCCAGT CAAAACTGAG 
AAGAAGGACA AAGCAGAAAA GGAAATCATA CCACAAGAAG ACAAAAAAGT CAAGGTTTCT 
CAGGTGGAAG AAAAGACTAA GGATGTCAAA GCTAAAACAT CAGAGAGAAA AGTAGCAAAA 
GACTCAAAGA AAGACGCTAA AGAGGAAGAA GAAATCAAGA CAAAGCCACA TACTAAAGTG 
GAAACACTGG ATGCAGCAGA AACAATGGGG GAAATAAAAG ATCAGTATAA TTTCTGCCGT 
TATGTGGTTG ACCTGTATTC TCATGGAGAT GCTTCTACAG AAGAAAAATC AAAGGAAAAA 
CCACAAGAGA AGAAAGAAGT TCTCCCACAA GAAAAACCAG GAGAATTGAA AATATCATTA 
CCAGAAAAGA AAGATATTCC TGAAACAAAG GAAACAGTCT TAGAGGAGGT GACAATTCAT 
GAAGCTGAAG ACGAATGCCC AAAGGAAGAT TATGATCATT TAACAGTTCA TAAAGATACA 
GAAGAAAGTC CAAAAAAAGA GCAAGCTGAA GTAAAAACAG AAGAGAAGAA AGAAGGAAGA 
AGCCAACAGC CTGCATTTAC CGGTGTATCT ACAGATTTTG GCATCATGCC TCGATTGATC 
ATAATCATGT CAGATTCACA TGGTAGGTTT GCTTCAAGAC CAGATGTCTC TGTAGATTGG 
ACCAAAAGAG AAATGTTACT TAAAAAAGAG GAAAAAGAAG CTGAACCAAT AACACAAGAA 
AAGATGGACT ATATAAAAGA AGCCCCAACT ACATATATAA TAGCAGAGGC TCCTGTGCAG 
ATTAATGTCA CAAAACCACC AGATGCAAAG AAAGAAATAC AAATGGAAAT GCCTGTACGA 
AAACTTGAAG AAGGACCAAA AGAAATAAAA ACAGAAATAC CAGTGGAGAT ACCTGAAGAA 
AAAACAAAAG ATGTAAAAGA AGTTGTAGTA ATACCAGTGG AAATGCCAGT GAAGAAAATT 
AAACCGGAAG AAGAGATACC AGTGGAAATG CCAGTGAAAA AACTTAAAGA ACCAGAAGAA 
ATACCAGTGG AAATACCAGT AGAGAAACCC AAAGAAGTAT CAAAAGCAAA AGAACAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCATTCAG 
AAGACTGAAC AAGAAGTGAA GGAAGTTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AGGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CCTTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAAAA AGAGGAACCA GTTAAGAAGG CTGAACCAGA AGTAAAGGAC 
ATTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCTGTAA AGAAACCTGA AGCAAAACCT GCAGAAGTAA AGAAAGAGGT GCCCATTAAG 
AAGACTGAAC CAGAAGTGAT GGAAGTTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCC TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAAAAAG AGGAACCAGT TAAGAAGGCT GAACCAGAAG TAAAGGACAT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCTGTAAAG 
AAACCTGAAG CAAAACCTGC AGAAGTAAAG AAAGAGGTGC CCATTAAGAA GACTGAACCA 
GAAGTGAAGG AAGTTAAGAA AGAGGTGCCA TTAAAGAAGC CCGAACCAAA ACCTGCAGAA 
GTTAAGAAAG AGGTGCCCTT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAAAAAGAG 
GAACCAGTTA AGAAGGCTGA ACCAGAAGTA AAGGACATTA AGAAAGAGGT GCCATTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAG AAAGAGGTGC CTGTAAAGAA ACCTGAAGCA 
AAACCTGCAG AAGTAAAGAA AGAGGTGCCC ATTAAGAAGA CTGAACCAGA AGTGAAGGAA 
GTTAAGAAAG AGGTGCCATT AAAGAAGCCC GAACCAAAAC CTGCAGAAGT TAAGAAAGAG 
GTGCCCTTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AAAAAGAGGA ACCAGTTAAG 
AAGGCTGAAC CAGAAGTAAA GGACATTAAG AAAGAGGTGC CATTAAAGAA GCCCGAACCA 
AAACCTGCAG AAGTTAAGAA AGAGGTGCCT GTAAAGAAAC CTGAAGCAAA ACCTGCAGAA 
GTAAAGAAAG AGGTGCCCAT TAAGAAGACT GAACCAGAAG TGAAGGAAGT TAAGAAAGAG 
GTGCCATTAA AGAAGCCCGA ACCAAAACCT GCAGAAGTTA AGAAAGAGGT GCCCTTAAAG 
AAGCCCGAAC CAAAACCTGC AGAAGTTAAA AAAGGTAAAT ATTGTAGGCT CTGTAAAACT 
CTGTCTATAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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trdn cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: