emsy cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	emsy
Entrez Gene ID	100136947
Full Name	EMSY, BRCA2-interacting transcriptional repressor
Synonyms	c11orf30,gl002
General protein information	Preferred Names EMSY, BRCA2-interacting transcriptional repressor Names BRCA2-interacting transcriptional repressor EMSY
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004912219.3	XP_004912276.1	BRCA2-interacting transcriptional repressor EMSY isoform X3
XM_004912220.3	XP_004912277.1	BRCA2-interacting transcriptional repressor EMSY isoform X4
XM_004912217.4	XP_004912274.1	BRCA2-interacting transcriptional repressor EMSY isoform X1
XM_004912218.4	XP_004912275.1	BRCA2-interacting transcriptional repressor EMSY isoform X2
XM_004912217.3	XP_004912274.1	BRCA2-interacting transcriptional repressor EMSY isoform X1
XM_004912220.4	XP_004912277.1	BRCA2-interacting transcriptional repressor EMSY isoform X4
XM_004912218.3	XP_004912275.1	BRCA2-interacting transcriptional repressor EMSY isoform X2
XM_004912219.4	XP_004912276.1	BRCA2-interacting transcriptional repressor EMSY isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	C11H11orf30	XP_003313266.1
Rattus norvegicus (Norway rat)	RGD1310712	XP_006229864.1
Homo sapiens (human)	C11orf30	NP_064578.2
Canis lupus familiaris (dog)	C21H11orf30	XP_005633532.1
Danio rerio (zebrafish)	zgc:56039	NP_956278.1
Bos taurus (cattle)	C15H11orf30	NP_001193751.1
Mus musculus (house mouse)	2210018M11Rik	NP_758484.2
Gallus gallus (chicken)	C11ORF30	XP_417282.4
	C14H11orf30	XP_001087550.2
Xenopus tropicalis (tropical clawed frog)	c11orf30	XP_004912275.1

Gene Ontology

The following emsy gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the emsy cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa53000	XM_004912217.4 Latest version!	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53001	XM_004912218.4 Latest version!	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa53002	XM_004912219.4 Latest version!	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa53003	XM_004912220.4 Latest version!	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa06524	XM_004912219.3 Latest version!	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa06525	XM_004912220.3 Latest version!	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa06522	XM_004912217.3 Latest version!	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa06523	XM_004912218.3 Latest version!	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

emsy ( XM_004912217.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912274.1 Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa53000
Clone ID Related Accession (Same CDS sequence)	XM_004912217.4
Accession Version	XM_004912217.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	BRCA2-interacting transcriptional repressor EMSY isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912217.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAATTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG TGAAAGAGAA AGTGGAAAAT AAACTACGAC AGCCGACTAT CGATCTAAGT 
CAAATGGCTG TTCCCATTCA GATGGCTGCA GATAAACGCC ACGCTGAAGG CAGCTCTATT 
GCCATGGTGG AGTCAGAGCT GGTGGCAGAA TACCTAAGTA CAGGCAAAAG ACAAATGGCT 
TCTACTAACA CCGTGAGCTC TGGGGATATG CCTCTCCCAT CCCTACTGCA AGTGAGCCAT 
CCATCGCAGC AGCTGCAGCA GGTCCAACCC ACGCGCACCC TATTGCAGCA TGTTAGCCAG 
TCCCAGACAG CATCGCAGGC TTCTGTGGTG GTGAAATCAA TCCCTACCTC CTCCAGTGGA 
GCCATCACTC ATATCATGCA ACAGCATTTT ACTAACTTTG TCTCACAGGC ACTGAGCAGT 
CACACAGCTT TCACCAAGAG AAGCGAGGAG ACTGTGACTG AAGAAGGGGA GGTGGAGGAA 
ATGGACACTT TAGACCCCCA AACGGGCATC CTGTACCGGG CCGCCATGGC ACAAAAGCAG 
CAGAAACAGG TTCATCTGCA GCAGGAAGGA GCTCATCTGC AAGTGAAGAC GGTGCAGTCC 
CTGCAGACCA AGCAGAAACA GATCATTCAG CTCCAGCCGG ATCAAATGCA GCACAAAATC 
CAACAGACGG CGCAGCTCTC CATCCGCCAG CAAAAGGTCA CTCCCATGCA GCAGGAGCAC 
GCACAGCGCC ACCTGGTGGT GAAAGAGAGA CACATCCCTA ACATTATGTC TCAGCCCCAG 
CAGACTGTAG TGCAAGTGCT GGCCGTTAAA ACCACCCAGC AGCTGCCCAA ACTGCAGCAG 
GTTCAAGGTC AACAAAAGAT TTACATGCAG ACTCCAATAC AAATGCCAGT CTCATCTGAG 
AAACAAGCTG TTACGCAGAT GACCCAGTCT ATCGTGTCCC CAGGATCAGC CGTTACAAAG 
ATCACCTTTG AGGGCCACCA ACCTCCCACC GTCTCCAAGG CATCTGTAAG CGACTGCCTG 
CCCAGACTGA CCCCCGCAGT CGCCAGCATC CTGCCTACTG TCCAGAGCTC TGAGAAGTCC 
TCCGTAGCCG ATATCCTGAA GATGTCTATG ATGGAGGCAG ACATTGACAC GAATGCCGAG 
CCCATGGTTG TTGATGCCCC CACCAAAGTT GCCCCCATTA TTAAACCCAT GCCCATGCTG 
ACTGAAGCTA CAGTAACCCA GGTTCCAAGG TTTTCTGTGC CAGTGACTGC TTCTGTTCCA 
CCGCCTCCAA AATGTTTCCC GGCCCCCATT ATAACGGCCG CGCCCCCTAC TGTGCTGCCT 
GGACCTCTCC CACCGCCACC CCCTCTTACA AGGAAAGTCG ACGTCCCCAT TGCCAGCCAG 
ATCATCCAGA TACAGAACGT TCAGCAAAAG AGAACTGAGG AAGTGCCTTC TGAAATAATT 
ATCCAGACCA TCCCTCAGTA TCATGCCATC CCTTGTCACT CCAGCTCCAA TGTGGTAGTG 
GAACCCAGTG GGCTCCTGGA GATGAGTAAC TTTACCAGCC AGAGGCTCGG GGAGGAAGAC 
GTCGTCTTGG CTCACGACGG GGAGAGTGGC AAAAGCCAAG CCCCTATCCA TATCGAGGAC 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912274.1
CDS	126..4151
Translation	MIGPSAGGRALCGGEARMPVVWPTLLDFSRDECKRILRKLELEAYAGVISALRAQGDLCKEKKDLLGELSKVLSISTERHRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRLVPLMPRLVPQTAFTATANAVANAAGQHNASLPDPAETGNKEVVVCYSYTSTTSTPTSTPVPSGSVATVKSPRPASPASNVVVLPSGSTVYVKSVNCSDDDEKPRKRRRTNSNSSSPVVLKEVPKPTTPVSKTITVPVTGSPKMSNIMQSIANSLPPHMSPVKITFTKPATQTTNTSTQKVIIVTTSPSSTFVPNILSKSHNYAAVTKLVPTSVITSATQKQPLVVTASQSSVVTSSSTSSSSPPLTSGSIAVTTVVSSTPSLVMSTVAPGASTSAVKAATNRLPSPKSLVTSPSQILAQLPKQYQQSPKHQIHTIPQQTQQLAQATAITNPGTITHPPTAQQPPMPAAMKPTIQIKQESGVKIITQQVQPSKILPKPVSTSLPNSSNAPIMVVSSNGAIMTTKLVTTPSGTQATYTRPTISSALGARMAATPGATYVKTTSGSIITVVPKSLATLGGKIISSNIVSGTTTKITTIPMTSKPNVIVVQKTTGKGTTIQGLPGKNVVTTLLNAGGDKTLQAMPAGTKPAIITATRPITKMIVTQPKGMGSTVQPATKIIPTKIVYGQQGKTQVLIKPKPVTFQATVMSEQTRQLVTETLQQASRVVEATSATIQEVKEDTESSSSSTESSQSSQDSQPVVHVIASRSQDWAEHEIAMDTTPTIIYQDIGGESQSATSTIKALMELQQTAVKEKVENKLRQPTIDLSQMAVPIQMAADKRHAEGSSIAMVESELVAEYLSTGKRQMASTNTVSSGDMPLPSLLQVSHPSQQLQQVQPTRTLLQHVSQSQTASQASVVVKSIPTSSSGAITHIMQQHFTNFVSQALSSHTAFTKRSEETVTEEGEVEEMDTLDPQTGILYRAAMAQKQQKQVHLQQEGAHLQVKTVQSLQTKQKQIIQLQPDQMQHKIQQTAQLSIRQQKVTPMQQEHAQRHLVVKERHIPNIMSQPQQTVVQVLAVKTTQQLPKLQQVQGQQKIYMQTPIQMPVSSEKQAVTQMTQSIVSPGSAVTKITFEGHQPPTVSKASVSDCLPRLTPAVASILPTVQSSEKSSVADILKMSMMEADIDTNAEPMVVDAPTKVAPIIKPMPMLTEATVTQVPRFSVPVTASVPPPPKCFPAPIITAAPPTVLPGPLPPPPPLTRKVDVPIASQIIQIQNVQQKRTEEVPSEIIIQTIPQYHAIPCHSSSNVVVEPSGLLEMSNFTSQRLGEEDVVLAHDGESGKSQAPIHIEDV

Target ORF information:

RefSeq Version	XM_004912217.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912217.4

ORF Insert Sequence:

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAATTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG TGAAAGAGAA AGTGGAAAAT AAACTACGAC AGCCGACTAT CGATCTAAGT 
CAAATGGCTG TTCCCATTCA GATGGCTGCA GATAAACGCC ACGCTGAAGG CAGCTCTATT 
GCCATGGTGG AGTCAGAGCT GGTGGCAGAA TACCTAAGTA CAGGCAAAAG ACAAATGGCT 
TCTACTAACA CCGTGAGCTC TGGGGATATG CCTCTCCCAT CCCTACTGCA AGTGAGCCAT 
CCATCGCAGC AGCTGCAGCA GGTCCAACCC ACGCGCACCC TATTGCAGCA TGTTAGCCAG 
TCCCAGACAG CATCGCAGGC TTCTGTGGTG GTGAAATCAA TCCCTACCTC CTCCAGTGGA 
GCCATCACTC ATATCATGCA ACAGCATTTT ACTAACTTTG TCTCACAGGC ACTGAGCAGT 
CACACAGCTT TCACCAAGAG AAGCGAGGAG ACTGTGACTG AAGAAGGGGA GGTGGAGGAA 
ATGGACACTT TAGACCCCCA AACGGGCATC CTGTACCGGG CCGCCATGGC ACAAAAGCAG 
CAGAAACAGG TTCATCTGCA GCAGGAAGGA GCTCATCTGC AAGTGAAGAC GGTGCAGTCC 
CTGCAGACCA AGCAGAAACA GATCATTCAG CTCCAGCCGG ATCAAATGCA GCACAAAATC 
CAACAGACGG CGCAGCTCTC CATCCGCCAG CAAAAGGTCA CTCCCATGCA GCAGGAGCAC 
GCACAGCGCC ACCTGGTGGT GAAAGAGAGA CACATCCCTA ACATTATGTC TCAGCCCCAG 
CAGACTGTAG TGCAAGTGCT GGCCGTTAAA ACCACCCAGC AGCTGCCCAA ACTGCAGCAG 
GTTCAAGGTC AACAAAAGAT TTACATGCAG ACTCCAATAC AAATGCCAGT CTCATCTGAG 
AAACAAGCTG TTACGCAGAT GACCCAGTCT ATCGTGTCCC CAGGATCAGC CGTTACAAAG 
ATCACCTTTG AGGGCCACCA ACCTCCCACC GTCTCCAAGG CATCTGTAAG CGACTGCCTG 
CCCAGACTGA CCCCCGCAGT CGCCAGCATC CTGCCTACTG TCCAGAGCTC TGAGAAGTCC 
TCCGTAGCCG ATATCCTGAA GATGTCTATG ATGGAGGCAG ACATTGACAC GAATGCCGAG 
CCCATGGTTG TTGATGCCCC CACCAAAGTT GCCCCCATTA TTAAACCCAT GCCCATGCTG 
ACTGAAGCTA CAGTAACCCA GGTTCCAAGG TTTTCTGTGC CAGTGACTGC TTCTGTTCCA 
CCGCCTCCAA AATGTTTCCC GGCCCCCATT ATAACGGCCG CGCCCCCTAC TGTGCTGCCT 
GGACCTCTCC CACCGCCACC CCCTCTTACA AGGAAAGTCG ACGTCCCCAT TGCCAGCCAG 
ATCATCCAGA TACAGAACGT TCAGCAAAAG AGAACTGAGG AAGTGCCTTC TGAAATAATT 
ATCCAGACCA TCCCTCAGTA TCATGCCATC CCTTGTCACT CCAGCTCCAA TGTGGTAGTG 
GAACCCAGTG GGCTCCTGGA GATGAGTAAC TTTACCAGCC AGAGGCTCGG GGAGGAAGAC 
GTCGTCTTGG CTCACGACGG GGAGAGTGGC AAAAGCCAAG CCCCTATCCA TATCGAGGAC 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emsy ( XM_004912218.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912275.1 Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa53001
Clone ID Related Accession (Same CDS sequence)	XM_004912218.4
Accession Version	XM_004912218.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	BRCA2-interacting transcriptional repressor EMSY isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912218.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAATTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG TGAAAGAGAA AGTGGAAAAT AAACTACGAC AGCCGACTAT CGATCTAAGT 
CAAATGGCTG TTCCCATTCA GATGGCTGCA GATAAACGCC ACGCTGAAGG CAGCTCTATT 
GCCATGGTGG AGTCAGAGCT GGTGGCAGAA TACCTAAGTA CAGGCAAAAG ACAAATGGCT 
TCTACTAACA CCGTGAGCTC TGGGGATATG CCTCTCCCAT CCCTACTGCA AGTGAGCCAT 
CCATCGCAGC AGCTGCAGCA GGTCCAACCC ACGCGCACCC TATTGCAGCA TGTTAGCCAG 
TCCCAGACAG CATCGCAGGC TTCTGTGGTG GTGAAATCAA TCCCTACCTC CTCCAGTGGA 
GCCATCACTC ATATCATGCA ACAGGCACTG AGCAGTCACA CAGCTTTCAC CAAGAGAAGC 
GAGGAGACTG TGACTGAAGA AGGGGAGGTG GAGGAAATGG ACACTTTAGA CCCCCAAACG 
GGCATCCTGT ACCGGGCCGC CATGGCACAA AAGCAGCAGA AACAGGTTCA TCTGCAGCAG 
GAAGGAGCTC ATCTGCAAGT GAAGACGGTG CAGTCCCTGC AGACCAAGCA GAAACAGATC 
ATTCAGCTCC AGCCGGATCA AATGCAGCAC AAAATCCAAC AGACGGCGCA GCTCTCCATC 
CGCCAGCAAA AGGTCACTCC CATGCAGCAG GAGCACGCAC AGCGCCACCT GGTGGTGAAA 
GAGAGACACA TCCCTAACAT TATGTCTCAG CCCCAGCAGA CTGTAGTGCA AGTGCTGGCC 
GTTAAAACCA CCCAGCAGCT GCCCAAACTG CAGCAGGTTC AAGGTCAACA AAAGATTTAC 
ATGCAGACTC CAATACAAAT GCCAGTCTCA TCTGAGAAAC AAGCTGTTAC GCAGATGACC 
CAGTCTATCG TGTCCCCAGG ATCAGCCGTT ACAAAGATCA CCTTTGAGGG CCACCAACCT 
CCCACCGTCT CCAAGGCATC TGTAAGCGAC TGCCTGCCCA GACTGACCCC CGCAGTCGCC 
AGCATCCTGC CTACTGTCCA GAGCTCTGAG AAGTCCTCCG TAGCCGATAT CCTGAAGATG 
TCTATGATGG AGGCAGACAT TGACACGAAT GCCGAGCCCA TGGTTGTTGA TGCCCCCACC 
AAAGTTGCCC CCATTATTAA ACCCATGCCC ATGCTGACTG AAGCTACAGT AACCCAGGTT 
CCAAGGTTTT CTGTGCCAGT GACTGCTTCT GTTCCACCGC CTCCAAAATG TTTCCCGGCC 
CCCATTATAA CGGCCGCGCC CCCTACTGTG CTGCCTGGAC CTCTCCCACC GCCACCCCCT 
CTTACAAGGA AAGTCGACGT CCCCATTGCC AGCCAGATCA TCCAGATACA GAACGTTCAG 
CAAAAGAGAA CTGAGGAAGT GCCTTCTGAA ATAATTATCC AGACCATCCC TCAGTATCAT 
GCCATCCCTT GTCACTCCAG CTCCAATGTG GTAGTGGAAC CCAGTGGGCT CCTGGAGATG 
AGTAACTTTA CCAGCCAGAG GCTCGGGGAG GAAGACGTCG TCTTGGCTCA CGACGGGGAG 
AGTGGCAAAA GCCAAGCCCC TATCCATATC GAGGACGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912275.1
CDS	126..4127
Translation	MIGPSAGGRALCGGEARMPVVWPTLLDFSRDECKRILRKLELEAYAGVISALRAQGDLCKEKKDLLGELSKVLSISTERHRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRLVPLMPRLVPQTAFTATANAVANAAGQHNASLPDPAETGNKEVVVCYSYTSTTSTPTSTPVPSGSVATVKSPRPASPASNVVVLPSGSTVYVKSVNCSDDDEKPRKRRRTNSNSSSPVVLKEVPKPTTPVSKTITVPVTGSPKMSNIMQSIANSLPPHMSPVKITFTKPATQTTNTSTQKVIIVTTSPSSTFVPNILSKSHNYAAVTKLVPTSVITSATQKQPLVVTASQSSVVTSSSTSSSSPPLTSGSIAVTTVVSSTPSLVMSTVAPGASTSAVKAATNRLPSPKSLVTSPSQILAQLPKQYQQSPKHQIHTIPQQTQQLAQATAITNPGTITHPPTAQQPPMPAAMKPTIQIKQESGVKIITQQVQPSKILPKPVSTSLPNSSNAPIMVVSSNGAIMTTKLVTTPSGTQATYTRPTISSALGARMAATPGATYVKTTSGSIITVVPKSLATLGGKIISSNIVSGTTTKITTIPMTSKPNVIVVQKTTGKGTTIQGLPGKNVVTTLLNAGGDKTLQAMPAGTKPAIITATRPITKMIVTQPKGMGSTVQPATKIIPTKIVYGQQGKTQVLIKPKPVTFQATVMSEQTRQLVTETLQQASRVVEATSATIQEVKEDTESSSSSTESSQSSQDSQPVVHVIASRSQDWAEHEIAMDTTPTIIYQDIGGESQSATSTIKALMELQQTAVKEKVENKLRQPTIDLSQMAVPIQMAADKRHAEGSSIAMVESELVAEYLSTGKRQMASTNTVSSGDMPLPSLLQVSHPSQQLQQVQPTRTLLQHVSQSQTASQASVVVKSIPTSSSGAITHIMQQALSSHTAFTKRSEETVTEEGEVEEMDTLDPQTGILYRAAMAQKQQKQVHLQQEGAHLQVKTVQSLQTKQKQIIQLQPDQMQHKIQQTAQLSIRQQKVTPMQQEHAQRHLVVKERHIPNIMSQPQQTVVQVLAVKTTQQLPKLQQVQGQQKIYMQTPIQMPVSSEKQAVTQMTQSIVSPGSAVTKITFEGHQPPTVSKASVSDCLPRLTPAVASILPTVQSSEKSSVADILKMSMMEADIDTNAEPMVVDAPTKVAPIIKPMPMLTEATVTQVPRFSVPVTASVPPPPKCFPAPIITAAPPTVLPGPLPPPPPLTRKVDVPIASQIIQIQNVQQKRTEEVPSEIIIQTIPQYHAIPCHSSSNVVVEPSGLLEMSNFTSQRLGEEDVVLAHDGESGKSQAPIHIEDV

Target ORF information:

RefSeq Version	XM_004912218.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912218.4

ORF Insert Sequence:

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAATTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG TGAAAGAGAA AGTGGAAAAT AAACTACGAC AGCCGACTAT CGATCTAAGT 
CAAATGGCTG TTCCCATTCA GATGGCTGCA GATAAACGCC ACGCTGAAGG CAGCTCTATT 
GCCATGGTGG AGTCAGAGCT GGTGGCAGAA TACCTAAGTA CAGGCAAAAG ACAAATGGCT 
TCTACTAACA CCGTGAGCTC TGGGGATATG CCTCTCCCAT CCCTACTGCA AGTGAGCCAT 
CCATCGCAGC AGCTGCAGCA GGTCCAACCC ACGCGCACCC TATTGCAGCA TGTTAGCCAG 
TCCCAGACAG CATCGCAGGC TTCTGTGGTG GTGAAATCAA TCCCTACCTC CTCCAGTGGA 
GCCATCACTC ATATCATGCA ACAGGCACTG AGCAGTCACA CAGCTTTCAC CAAGAGAAGC 
GAGGAGACTG TGACTGAAGA AGGGGAGGTG GAGGAAATGG ACACTTTAGA CCCCCAAACG 
GGCATCCTGT ACCGGGCCGC CATGGCACAA AAGCAGCAGA AACAGGTTCA TCTGCAGCAG 
GAAGGAGCTC ATCTGCAAGT GAAGACGGTG CAGTCCCTGC AGACCAAGCA GAAACAGATC 
ATTCAGCTCC AGCCGGATCA AATGCAGCAC AAAATCCAAC AGACGGCGCA GCTCTCCATC 
CGCCAGCAAA AGGTCACTCC CATGCAGCAG GAGCACGCAC AGCGCCACCT GGTGGTGAAA 
GAGAGACACA TCCCTAACAT TATGTCTCAG CCCCAGCAGA CTGTAGTGCA AGTGCTGGCC 
GTTAAAACCA CCCAGCAGCT GCCCAAACTG CAGCAGGTTC AAGGTCAACA AAAGATTTAC 
ATGCAGACTC CAATACAAAT GCCAGTCTCA TCTGAGAAAC AAGCTGTTAC GCAGATGACC 
CAGTCTATCG TGTCCCCAGG ATCAGCCGTT ACAAAGATCA CCTTTGAGGG CCACCAACCT 
CCCACCGTCT CCAAGGCATC TGTAAGCGAC TGCCTGCCCA GACTGACCCC CGCAGTCGCC 
AGCATCCTGC CTACTGTCCA GAGCTCTGAG AAGTCCTCCG TAGCCGATAT CCTGAAGATG 
TCTATGATGG AGGCAGACAT TGACACGAAT GCCGAGCCCA TGGTTGTTGA TGCCCCCACC 
AAAGTTGCCC CCATTATTAA ACCCATGCCC ATGCTGACTG AAGCTACAGT AACCCAGGTT 
CCAAGGTTTT CTGTGCCAGT GACTGCTTCT GTTCCACCGC CTCCAAAATG TTTCCCGGCC 
CCCATTATAA CGGCCGCGCC CCCTACTGTG CTGCCTGGAC CTCTCCCACC GCCACCCCCT 
CTTACAAGGA AAGTCGACGT CCCCATTGCC AGCCAGATCA TCCAGATACA GAACGTTCAG 
CAAAAGAGAA CTGAGGAAGT GCCTTCTGAA ATAATTATCC AGACCATCCC TCAGTATCAT 
GCCATCCCTT GTCACTCCAG CTCCAATGTG GTAGTGGAAC CCAGTGGGCT CCTGGAGATG 
AGTAACTTTA CCAGCCAGAG GCTCGGGGAG GAAGACGTCG TCTTGGCTCA CGACGGGGAG 
AGTGGCAAAA GCCAAGCCCC TATCCATATC GAGGACGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emsy ( XM_004912219.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912276.1 Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X3, mRNA.

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CloneID	OXa53002
Clone ID Related Accession (Same CDS sequence)	XM_004912219.4
Accession Version	XM_004912219.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	BRCA2-interacting transcriptional repressor EMSY isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912219.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAATTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG GCAAAAGACA AATGGCTTCT ACTAACACCG TGAGCTCTGG GGATATGCCT 
CTCCCATCCC TACTGCAAGT GAGCCATCCA TCGCAGCAGC TGCAGCAGGT CCAACCCACG 
CGCACCCTAT TGCAGCATGT TAGCCAGTCC CAGACAGCAT CGCAGGCTTC TGTGGTGGTG 
AAATCAATCC CTACCTCCTC CAGTGGAGCC ATCACTCATA TCATGCAACA GCATTTTACT 
AACTTTGTCT CACAGGCACT GAGCAGTCAC ACAGCTTTCA CCAAGAGAAG CGAGGAGACT 
GTGACTGAAG AAGGGGAGGT GGAGGAAATG GACACTTTAG ACCCCCAAAC GGGCATCCTG 
TACCGGGCCG CCATGGCACA AAAGCAGCAG AAACAGGTTC ATCTGCAGCA GGAAGGAGCT 
CATCTGCAAG TGAAGACGGT GCAGTCCCTG CAGACCAAGC AGAAACAGAT CATTCAGCTC 
CAGCCGGATC AAATGCAGCA CAAAATCCAA CAGACGGCGC AGCTCTCCAT CCGCCAGCAA 
AAGGTCACTC CCATGCAGCA GGAGCACGCA CAGCGCCACC TGGTGGTGAA AGAGAGACAC 
ATCCCTAACA TTATGTCTCA GCCCCAGCAG ACTGTAGTGC AAGTGCTGGC CGTTAAAACC 
ACCCAGCAGC TGCCCAAACT GCAGCAGGTT CAAGGTCAAC AAAAGATTTA CATGCAGACT 
CCAATACAAA TGCCAGTCTC ATCTGAGAAA CAAGCTGTTA CGCAGATGAC CCAGTCTATC 
GTGTCCCCAG GATCAGCCGT TACAAAGATC ACCTTTGAGG GCCACCAACC TCCCACCGTC 
TCCAAGGCAT CTGTAAGCGA CTGCCTGCCC AGACTGACCC CCGCAGTCGC CAGCATCCTG 
CCTACTGTCC AGAGCTCTGA GAAGTCCTCC GTAGCCGATA TCCTGAAGAT GTCTATGATG 
GAGGCAGACA TTGACACGAA TGCCGAGCCC ATGGTTGTTG ATGCCCCCAC CAAAGTTGCC 
CCCATTATTA AACCCATGCC CATGCTGACT GAAGCTACAG TAACCCAGGT TCCAAGGTTT 
TCTGTGCCAG TGACTGCTTC TGTTCCACCG CCTCCAAAAT GTTTCCCGGC CCCCATTATA 
ACGGCCGCGC CCCCTACTGT GCTGCCTGGA CCTCTCCCAC CGCCACCCCC TCTTACAAGG 
AAAGTCGACG TCCCCATTGC CAGCCAGATC ATCCAGATAC AGAACGTTCA GCAAAAGAGA 
ACTGAGGAAG TGCCTTCTGA AATAATTATC CAGACCATCC CTCAGTATCA TGCCATCCCT 
TGTCACTCCA GCTCCAATGT GGTAGTGGAA CCCAGTGGGC TCCTGGAGAT GAGTAACTTT 
ACCAGCCAGA GGCTCGGGGA GGAAGACGTC GTCTTGGCTC ACGACGGGGA GAGTGGCAAA 
AGCCAAGCCC CTATCCATAT CGAGGACGTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912276.1
CDS	126..3998
Translation	MIGPSAGGRALCGGEARMPVVWPTLLDFSRDECKRILRKLELEAYAGVISALRAQGDLCKEKKDLLGELSKVLSISTERHRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRLVPLMPRLVPQTAFTATANAVANAAGQHNASLPDPAETGNKEVVVCYSYTSTTSTPTSTPVPSGSVATVKSPRPASPASNVVVLPSGSTVYVKSVNCSDDDEKPRKRRRTNSNSSSPVVLKEVPKPTTPVSKTITVPVTGSPKMSNIMQSIANSLPPHMSPVKITFTKPATQTTNTSTQKVIIVTTSPSSTFVPNILSKSHNYAAVTKLVPTSVITSATQKQPLVVTASQSSVVTSSSTSSSSPPLTSGSIAVTTVVSSTPSLVMSTVAPGASTSAVKAATNRLPSPKSLVTSPSQILAQLPKQYQQSPKHQIHTIPQQTQQLAQATAITNPGTITHPPTAQQPPMPAAMKPTIQIKQESGVKIITQQVQPSKILPKPVSTSLPNSSNAPIMVVSSNGAIMTTKLVTTPSGTQATYTRPTISSALGARMAATPGATYVKTTSGSIITVVPKSLATLGGKIISSNIVSGTTTKITTIPMTSKPNVIVVQKTTGKGTTIQGLPGKNVVTTLLNAGGDKTLQAMPAGTKPAIITATRPITKMIVTQPKGMGSTVQPATKIIPTKIVYGQQGKTQVLIKPKPVTFQATVMSEQTRQLVTETLQQASRVVEATSATIQEVKEDTESSSSSTESSQSSQDSQPVVHVIASRSQDWAEHEIAMDTTPTIIYQDIGGESQSATSTIKALMELQQTAGKRQMASTNTVSSGDMPLPSLLQVSHPSQQLQQVQPTRTLLQHVSQSQTASQASVVVKSIPTSSSGAITHIMQQHFTNFVSQALSSHTAFTKRSEETVTEEGEVEEMDTLDPQTGILYRAAMAQKQQKQVHLQQEGAHLQVKTVQSLQTKQKQIIQLQPDQMQHKIQQTAQLSIRQQKVTPMQQEHAQRHLVVKERHIPNIMSQPQQTVVQVLAVKTTQQLPKLQQVQGQQKIYMQTPIQMPVSSEKQAVTQMTQSIVSPGSAVTKITFEGHQPPTVSKASVSDCLPRLTPAVASILPTVQSSEKSSVADILKMSMMEADIDTNAEPMVVDAPTKVAPIIKPMPMLTEATVTQVPRFSVPVTASVPPPPKCFPAPIITAAPPTVLPGPLPPPPPLTRKVDVPIASQIIQIQNVQQKRTEEVPSEIIIQTIPQYHAIPCHSSSNVVVEPSGLLEMSNFTSQRLGEEDVVLAHDGESGKSQAPIHIEDV

Target ORF information:

RefSeq Version	XM_004912219.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912219.4

ORF Insert Sequence:

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAATTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG GCAAAAGACA AATGGCTTCT ACTAACACCG TGAGCTCTGG GGATATGCCT 
CTCCCATCCC TACTGCAAGT GAGCCATCCA TCGCAGCAGC TGCAGCAGGT CCAACCCACG 
CGCACCCTAT TGCAGCATGT TAGCCAGTCC CAGACAGCAT CGCAGGCTTC TGTGGTGGTG 
AAATCAATCC CTACCTCCTC CAGTGGAGCC ATCACTCATA TCATGCAACA GCATTTTACT 
AACTTTGTCT CACAGGCACT GAGCAGTCAC ACAGCTTTCA CCAAGAGAAG CGAGGAGACT 
GTGACTGAAG AAGGGGAGGT GGAGGAAATG GACACTTTAG ACCCCCAAAC GGGCATCCTG 
TACCGGGCCG CCATGGCACA AAAGCAGCAG AAACAGGTTC ATCTGCAGCA GGAAGGAGCT 
CATCTGCAAG TGAAGACGGT GCAGTCCCTG CAGACCAAGC AGAAACAGAT CATTCAGCTC 
CAGCCGGATC AAATGCAGCA CAAAATCCAA CAGACGGCGC AGCTCTCCAT CCGCCAGCAA 
AAGGTCACTC CCATGCAGCA GGAGCACGCA CAGCGCCACC TGGTGGTGAA AGAGAGACAC 
ATCCCTAACA TTATGTCTCA GCCCCAGCAG ACTGTAGTGC AAGTGCTGGC CGTTAAAACC 
ACCCAGCAGC TGCCCAAACT GCAGCAGGTT CAAGGTCAAC AAAAGATTTA CATGCAGACT 
CCAATACAAA TGCCAGTCTC ATCTGAGAAA CAAGCTGTTA CGCAGATGAC CCAGTCTATC 
GTGTCCCCAG GATCAGCCGT TACAAAGATC ACCTTTGAGG GCCACCAACC TCCCACCGTC 
TCCAAGGCAT CTGTAAGCGA CTGCCTGCCC AGACTGACCC CCGCAGTCGC CAGCATCCTG 
CCTACTGTCC AGAGCTCTGA GAAGTCCTCC GTAGCCGATA TCCTGAAGAT GTCTATGATG 
GAGGCAGACA TTGACACGAA TGCCGAGCCC ATGGTTGTTG ATGCCCCCAC CAAAGTTGCC 
CCCATTATTA AACCCATGCC CATGCTGACT GAAGCTACAG TAACCCAGGT TCCAAGGTTT 
TCTGTGCCAG TGACTGCTTC TGTTCCACCG CCTCCAAAAT GTTTCCCGGC CCCCATTATA 
ACGGCCGCGC CCCCTACTGT GCTGCCTGGA CCTCTCCCAC CGCCACCCCC TCTTACAAGG 
AAAGTCGACG TCCCCATTGC CAGCCAGATC ATCCAGATAC AGAACGTTCA GCAAAAGAGA 
ACTGAGGAAG TGCCTTCTGA AATAATTATC CAGACCATCC CTCAGTATCA TGCCATCCCT 
TGTCACTCCA GCTCCAATGT GGTAGTGGAA CCCAGTGGGC TCCTGGAGAT GAGTAACTTT 
ACCAGCCAGA GGCTCGGGGA GGAAGACGTC GTCTTGGCTC ACGACGGGGA GAGTGGCAAA 
AGCCAAGCCC CTATCCATAT CGAGGACGTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emsy ( XM_004912220.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912277.1 Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa53003
Clone ID Related Accession (Same CDS sequence)	XM_004912220.4
Accession Version	XM_004912220.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	BRCA2-interacting transcriptional repressor EMSY isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912220.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAATTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG GCAAAAGACA AATGGCTTCT ACTAACACCG TGAGCTCTGG GGATATGCCT 
CTCCCATCCC TACTGCAAGT GAGCCATCCA TCGCAGCAGC TGCAGCAGGT CCAACCCACG 
CGCACCCTAT TGCAGCATGT TAGCCAGTCC CAGACAGCAT CGCAGGCTTC TGTGGTGGTG 
AAATCAATCC CTACCTCCTC CAGTGGAGCC ATCACTCATA TCATGCAACA GGCACTGAGC 
AGTCACACAG CTTTCACCAA GAGAAGCGAG GAGACTGTGA CTGAAGAAGG GGAGGTGGAG 
GAAATGGACA CTTTAGACCC CCAAACGGGC ATCCTGTACC GGGCCGCCAT GGCACAAAAG 
CAGCAGAAAC AGGTTCATCT GCAGCAGGAA GGAGCTCATC TGCAAGTGAA GACGGTGCAG 
TCCCTGCAGA CCAAGCAGAA ACAGATCATT CAGCTCCAGC CGGATCAAAT GCAGCACAAA 
ATCCAACAGA CGGCGCAGCT CTCCATCCGC CAGCAAAAGG TCACTCCCAT GCAGCAGGAG 
CACGCACAGC GCCACCTGGT GGTGAAAGAG AGACACATCC CTAACATTAT GTCTCAGCCC 
CAGCAGACTG TAGTGCAAGT GCTGGCCGTT AAAACCACCC AGCAGCTGCC CAAACTGCAG 
CAGGTTCAAG GTCAACAAAA GATTTACATG CAGACTCCAA TACAAATGCC AGTCTCATCT 
GAGAAACAAG CTGTTACGCA GATGACCCAG TCTATCGTGT CCCCAGGATC AGCCGTTACA 
AAGATCACCT TTGAGGGCCA CCAACCTCCC ACCGTCTCCA AGGCATCTGT AAGCGACTGC 
CTGCCCAGAC TGACCCCCGC AGTCGCCAGC ATCCTGCCTA CTGTCCAGAG CTCTGAGAAG 
TCCTCCGTAG CCGATATCCT GAAGATGTCT ATGATGGAGG CAGACATTGA CACGAATGCC 
GAGCCCATGG TTGTTGATGC CCCCACCAAA GTTGCCCCCA TTATTAAACC CATGCCCATG 
CTGACTGAAG CTACAGTAAC CCAGGTTCCA AGGTTTTCTG TGCCAGTGAC TGCTTCTGTT 
CCACCGCCTC CAAAATGTTT CCCGGCCCCC ATTATAACGG CCGCGCCCCC TACTGTGCTG 
CCTGGACCTC TCCCACCGCC ACCCCCTCTT ACAAGGAAAG TCGACGTCCC CATTGCCAGC 
CAGATCATCC AGATACAGAA CGTTCAGCAA AAGAGAACTG AGGAAGTGCC TTCTGAAATA 
ATTATCCAGA CCATCCCTCA GTATCATGCC ATCCCTTGTC ACTCCAGCTC CAATGTGGTA 
GTGGAACCCA GTGGGCTCCT GGAGATGAGT AACTTTACCA GCCAGAGGCT CGGGGAGGAA 
GACGTCGTCT TGGCTCACGA CGGGGAGAGT GGCAAAAGCC AAGCCCCTAT CCATATCGAG 
GACGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912277.1
CDS	126..3974
Translation	MIGPSAGGRALCGGEARMPVVWPTLLDFSRDECKRILRKLELEAYAGVISALRAQGDLCKEKKDLLGELSKVLSISTERHRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRLVPLMPRLVPQTAFTATANAVANAAGQHNASLPDPAETGNKEVVVCYSYTSTTSTPTSTPVPSGSVATVKSPRPASPASNVVVLPSGSTVYVKSVNCSDDDEKPRKRRRTNSNSSSPVVLKEVPKPTTPVSKTITVPVTGSPKMSNIMQSIANSLPPHMSPVKITFTKPATQTTNTSTQKVIIVTTSPSSTFVPNILSKSHNYAAVTKLVPTSVITSATQKQPLVVTASQSSVVTSSSTSSSSPPLTSGSIAVTTVVSSTPSLVMSTVAPGASTSAVKAATNRLPSPKSLVTSPSQILAQLPKQYQQSPKHQIHTIPQQTQQLAQATAITNPGTITHPPTAQQPPMPAAMKPTIQIKQESGVKIITQQVQPSKILPKPVSTSLPNSSNAPIMVVSSNGAIMTTKLVTTPSGTQATYTRPTISSALGARMAATPGATYVKTTSGSIITVVPKSLATLGGKIISSNIVSGTTTKITTIPMTSKPNVIVVQKTTGKGTTIQGLPGKNVVTTLLNAGGDKTLQAMPAGTKPAIITATRPITKMIVTQPKGMGSTVQPATKIIPTKIVYGQQGKTQVLIKPKPVTFQATVMSEQTRQLVTETLQQASRVVEATSATIQEVKEDTESSSSSTESSQSSQDSQPVVHVIASRSQDWAEHEIAMDTTPTIIYQDIGGESQSATSTIKALMELQQTAGKRQMASTNTVSSGDMPLPSLLQVSHPSQQLQQVQPTRTLLQHVSQSQTASQASVVVKSIPTSSSGAITHIMQQALSSHTAFTKRSEETVTEEGEVEEMDTLDPQTGILYRAAMAQKQQKQVHLQQEGAHLQVKTVQSLQTKQKQIIQLQPDQMQHKIQQTAQLSIRQQKVTPMQQEHAQRHLVVKERHIPNIMSQPQQTVVQVLAVKTTQQLPKLQQVQGQQKIYMQTPIQMPVSSEKQAVTQMTQSIVSPGSAVTKITFEGHQPPTVSKASVSDCLPRLTPAVASILPTVQSSEKSSVADILKMSMMEADIDTNAEPMVVDAPTKVAPIIKPMPMLTEATVTQVPRFSVPVTASVPPPPKCFPAPIITAAPPTVLPGPLPPPPPLTRKVDVPIASQIIQIQNVQQKRTEEVPSEIIIQTIPQYHAIPCHSSSNVVVEPSGLLEMSNFTSQRLGEEDVVLAHDGESGKSQAPIHIEDV

Target ORF information:

RefSeq Version	XM_004912220.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912220.4

ORF Insert Sequence:

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAATTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG GCAAAAGACA AATGGCTTCT ACTAACACCG TGAGCTCTGG GGATATGCCT 
CTCCCATCCC TACTGCAAGT GAGCCATCCA TCGCAGCAGC TGCAGCAGGT CCAACCCACG 
CGCACCCTAT TGCAGCATGT TAGCCAGTCC CAGACAGCAT CGCAGGCTTC TGTGGTGGTG 
AAATCAATCC CTACCTCCTC CAGTGGAGCC ATCACTCATA TCATGCAACA GGCACTGAGC 
AGTCACACAG CTTTCACCAA GAGAAGCGAG GAGACTGTGA CTGAAGAAGG GGAGGTGGAG 
GAAATGGACA CTTTAGACCC CCAAACGGGC ATCCTGTACC GGGCCGCCAT GGCACAAAAG 
CAGCAGAAAC AGGTTCATCT GCAGCAGGAA GGAGCTCATC TGCAAGTGAA GACGGTGCAG 
TCCCTGCAGA CCAAGCAGAA ACAGATCATT CAGCTCCAGC CGGATCAAAT GCAGCACAAA 
ATCCAACAGA CGGCGCAGCT CTCCATCCGC CAGCAAAAGG TCACTCCCAT GCAGCAGGAG 
CACGCACAGC GCCACCTGGT GGTGAAAGAG AGACACATCC CTAACATTAT GTCTCAGCCC 
CAGCAGACTG TAGTGCAAGT GCTGGCCGTT AAAACCACCC AGCAGCTGCC CAAACTGCAG 
CAGGTTCAAG GTCAACAAAA GATTTACATG CAGACTCCAA TACAAATGCC AGTCTCATCT 
GAGAAACAAG CTGTTACGCA GATGACCCAG TCTATCGTGT CCCCAGGATC AGCCGTTACA 
AAGATCACCT TTGAGGGCCA CCAACCTCCC ACCGTCTCCA AGGCATCTGT AAGCGACTGC 
CTGCCCAGAC TGACCCCCGC AGTCGCCAGC ATCCTGCCTA CTGTCCAGAG CTCTGAGAAG 
TCCTCCGTAG CCGATATCCT GAAGATGTCT ATGATGGAGG CAGACATTGA CACGAATGCC 
GAGCCCATGG TTGTTGATGC CCCCACCAAA GTTGCCCCCA TTATTAAACC CATGCCCATG 
CTGACTGAAG CTACAGTAAC CCAGGTTCCA AGGTTTTCTG TGCCAGTGAC TGCTTCTGTT 
CCACCGCCTC CAAAATGTTT CCCGGCCCCC ATTATAACGG CCGCGCCCCC TACTGTGCTG 
CCTGGACCTC TCCCACCGCC ACCCCCTCTT ACAAGGAAAG TCGACGTCCC CATTGCCAGC 
CAGATCATCC AGATACAGAA CGTTCAGCAA AAGAGAACTG AGGAAGTGCC TTCTGAAATA 
ATTATCCAGA CCATCCCTCA GTATCATGCC ATCCCTTGTC ACTCCAGCTC CAATGTGGTA 
GTGGAACCCA GTGGGCTCCT GGAGATGAGT AACTTTACCA GCCAGAGGCT CGGGGAGGAA 
GACGTCGTCT TGGCTCACGA CGGGGAGAGT GGCAAAAGCC AAGCCCCTAT CCATATCGAG 
GACGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emsy ( XM_004912219.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912276.1 Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa06524
Clone ID Related Accession (Same CDS sequence)	XM_004912219.3
Accession Version	XM_004912219.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	BRCA2-interacting transcriptional repressor EMSY isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912219.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAACTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG GCAAAAGACA AATGGCTTCT ACTAACACCG TGAGCTCTGG GGATATGCCT 
CTCCCATCCC TACTGCAAGT GAGCCATCCA TCGCAGCAGC TGCAGCAGGT CCAACCCACG 
CGCACCCTAT TGCAGCATGT TAGCCAGTCC CAGACAGCAT CGCAGGCTTC TGTGGTGGTG 
AAATCAATCC CTACCTCCTC CAGTGGAGCC ATCACTCATA TCATGCAACA GCATTTTACT 
AACTTTGTCT CACAGGCACT GAGCAGTCAC ACAGCTTTCA CCAAGAGAAG CGAGGAGACT 
GTGACTGAAG AAGGGGAGGT GGAGGAAATG GACACTTTAG ACCCCCAAAC GGGCATCCTG 
TACCGGGCCG CCATGGCACA AAAGCAGCAG AAACAGGTTC ATCTGCAGCA GGAAGGAGCT 
CATCTGCAAG TGAAGACGGT GCAGTCCCTG CAGACCAAGC AGAAACAGAT CATTCAGCTC 
CAGCCGGATC AAATGCAGCA CAAAATCCAA CAGACGGCGC AGCTCTCCAT CCGCCAGCAA 
AAGGTCACTC CCATGCAGCA GGAGCACGCA CAGCGCCACC TGGTGGTGAA AGAGAGACAC 
ATCCCTAACA TTATGTCTCA GCCCCAGCAG ACTGTAGTGC AAGTGCTGGC CGTTAAAACC 
ACCCAGCAGC TGCCCAAACT GCAGCAGGTT CAAGGTCAAC AAAAGATTTA CATGCAGACT 
CCAATACAAA TGCCAGTCTC ATCTGAGAAA CAAGCTGTTA CGCAGATGAC CCAGTCTATC 
GTGTCCCCAG GATCAGCCGT TACAAAGATC ACCTTTGAGG GCCACCAACC TCCCACCGTC 
TCCAAGGCAT CTGTAAGCGA CTGCCTGCCC AGACTGACCC CCGCAGTCGC CAGCATCCTG 
CCTACTGTCC AGAGCTCTGA GAAGTCCTCC GTAGCCGATA TCCTGAAGAT GTCTATGATG 
GAGGCAGACA TTGACACGAA TGCCGAGCCC ATGGTTGTTG ATGCCCCCAC CAAAGTTGCC 
CCCATTATTA AACCCATGCC CATGCTGACT GAAGCTACAG TAACCCAGGT TCCAAGGTTT 
TCTGTGCCAG TGACTGCTTC TGTTCCACCG CCTCCAAAAT GTTTCCCGGC CCCCATTATA 
ACGGCCGCGC CCCCTACTGT GCTGCCTGGA CCTCTCCCAC CGCCACCCCC TCTTACAAGG 
AAAGTCGACG TCCCCATTGC CAGCCAGATC ATCCAGATAC AGAACGTTCA GCAAAAGAGA 
ACTGAGGAAG TGCCTTCTGA AATAATTATC CAGACCATCC CTCAGTATCA TGCCATCCCT 
TGTCACTCCA GCTCCAATGT GGTAGTGGAA CCCAGTGGGC TCCTGGAGAT GAGTAACTTT 
ACCAGCCAGA GGCTCGGGGA GGAAGACGTC GTCTTGGCTC ACGACGGGGA GAGTGGCAAA 
AGCCAAGCCC CTATCCATAT CGAGGACGTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912276.1
CDS	440..4312
Translation	MIGPSAGGRALCGGEARMPVVWPTLLDFSRDECKRILRKLELEAYAGVISALRAQGDLCKEKKDLLGELSKVLSISTERHRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRLVPLMPRLVPQTAFTATANAVANAAGQHNASLPDPAETGNKEVVVCYSYTSTTSTPTSTPVPSGSVATVKSPRPASPASNVVVLPSGSTVYVKSVNCSDDDEKPRKRRRTNSNSSSPVVLKEVPKPTTPVSKTITVPVTGSPKMSNIMQSIANSLPPHMSPVKITFTKPATQTTNTSTQKVIIVTTSPSSTFVPNILSKSHNYAAVTKLVPTSVITSATQKQPLVVTASQSSVVTSSSTSSSSPPLTSGSIAVTTVVSSTPSLVMSTVAPGASTSAVKAATNRLPSPKSLVTSPSQILAQLPKQYQQSPKHQIHTIPQQTQQLAQATAITNPGTITHPPTAQQPPMPAAMKPTIQIKQESGVKIITQQVQPSKILPKPVSTSLPNSSNAPIMVVSSNGAIMTTKLVTTPSGTQATYTRPTISSALGARMAATPGATYVKTTSGSIITVVPKSLATLGGKIISSNIVSGTTTKITTIPMTSKPNVIVVQKTTGKGTTIQGLPGKNVVTTLLNAGGDKTLQAMPAGTKPAIITATRPITKMIVTQPKGMGSTVQPATKIIPTKIVYGQQGKTQVLIKPKPVTFQATVMSEQTRQLVTETLQQASRVVEATSATIQEVKEDTESSSSSTESSQSSQDSQPVVHVIASRSQDWAEHEIAMDTTPTIIYQDIGGESQSATSTIKALMELQQTAGKRQMASTNTVSSGDMPLPSLLQVSHPSQQLQQVQPTRTLLQHVSQSQTASQASVVVKSIPTSSSGAITHIMQQHFTNFVSQALSSHTAFTKRSEETVTEEGEVEEMDTLDPQTGILYRAAMAQKQQKQVHLQQEGAHLQVKTVQSLQTKQKQIIQLQPDQMQHKIQQTAQLSIRQQKVTPMQQEHAQRHLVVKERHIPNIMSQPQQTVVQVLAVKTTQQLPKLQQVQGQQKIYMQTPIQMPVSSEKQAVTQMTQSIVSPGSAVTKITFEGHQPPTVSKASVSDCLPRLTPAVASILPTVQSSEKSSVADILKMSMMEADIDTNAEPMVVDAPTKVAPIIKPMPMLTEATVTQVPRFSVPVTASVPPPPKCFPAPIITAAPPTVLPGPLPPPPPLTRKVDVPIASQIIQIQNVQQKRTEEVPSEIIIQTIPQYHAIPCHSSSNVVVEPSGLLEMSNFTSQRLGEEDVVLAHDGESGKSQAPIHIEDV

Target ORF information:

RefSeq Version	XM_004912219.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912219.3

ORF Insert Sequence:

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAACTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG GCAAAAGACA AATGGCTTCT ACTAACACCG TGAGCTCTGG GGATATGCCT 
CTCCCATCCC TACTGCAAGT GAGCCATCCA TCGCAGCAGC TGCAGCAGGT CCAACCCACG 
CGCACCCTAT TGCAGCATGT TAGCCAGTCC CAGACAGCAT CGCAGGCTTC TGTGGTGGTG 
AAATCAATCC CTACCTCCTC CAGTGGAGCC ATCACTCATA TCATGCAACA GCATTTTACT 
AACTTTGTCT CACAGGCACT GAGCAGTCAC ACAGCTTTCA CCAAGAGAAG CGAGGAGACT 
GTGACTGAAG AAGGGGAGGT GGAGGAAATG GACACTTTAG ACCCCCAAAC GGGCATCCTG 
TACCGGGCCG CCATGGCACA AAAGCAGCAG AAACAGGTTC ATCTGCAGCA GGAAGGAGCT 
CATCTGCAAG TGAAGACGGT GCAGTCCCTG CAGACCAAGC AGAAACAGAT CATTCAGCTC 
CAGCCGGATC AAATGCAGCA CAAAATCCAA CAGACGGCGC AGCTCTCCAT CCGCCAGCAA 
AAGGTCACTC CCATGCAGCA GGAGCACGCA CAGCGCCACC TGGTGGTGAA AGAGAGACAC 
ATCCCTAACA TTATGTCTCA GCCCCAGCAG ACTGTAGTGC AAGTGCTGGC CGTTAAAACC 
ACCCAGCAGC TGCCCAAACT GCAGCAGGTT CAAGGTCAAC AAAAGATTTA CATGCAGACT 
CCAATACAAA TGCCAGTCTC ATCTGAGAAA CAAGCTGTTA CGCAGATGAC CCAGTCTATC 
GTGTCCCCAG GATCAGCCGT TACAAAGATC ACCTTTGAGG GCCACCAACC TCCCACCGTC 
TCCAAGGCAT CTGTAAGCGA CTGCCTGCCC AGACTGACCC CCGCAGTCGC CAGCATCCTG 
CCTACTGTCC AGAGCTCTGA GAAGTCCTCC GTAGCCGATA TCCTGAAGAT GTCTATGATG 
GAGGCAGACA TTGACACGAA TGCCGAGCCC ATGGTTGTTG ATGCCCCCAC CAAAGTTGCC 
CCCATTATTA AACCCATGCC CATGCTGACT GAAGCTACAG TAACCCAGGT TCCAAGGTTT 
TCTGTGCCAG TGACTGCTTC TGTTCCACCG CCTCCAAAAT GTTTCCCGGC CCCCATTATA 
ACGGCCGCGC CCCCTACTGT GCTGCCTGGA CCTCTCCCAC CGCCACCCCC TCTTACAAGG 
AAAGTCGACG TCCCCATTGC CAGCCAGATC ATCCAGATAC AGAACGTTCA GCAAAAGAGA 
ACTGAGGAAG TGCCTTCTGA AATAATTATC CAGACCATCC CTCAGTATCA TGCCATCCCT 
TGTCACTCCA GCTCCAATGT GGTAGTGGAA CCCAGTGGGC TCCTGGAGAT GAGTAACTTT 
ACCAGCCAGA GGCTCGGGGA GGAAGACGTC GTCTTGGCTC ACGACGGGGA GAGTGGCAAA 
AGCCAAGCCC CTATCCATAT CGAGGACGTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emsy ( XM_004912220.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912277.1 Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X4, mRNA.

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CloneID	OXa06525
Clone ID Related Accession (Same CDS sequence)	XM_004912220.3
Accession Version	XM_004912220.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	BRCA2-interacting transcriptional repressor EMSY isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912220.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAACTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG GCAAAAGACA AATGGCTTCT ACTAACACCG TGAGCTCTGG GGATATGCCT 
CTCCCATCCC TACTGCAAGT GAGCCATCCA TCGCAGCAGC TGCAGCAGGT CCAACCCACG 
CGCACCCTAT TGCAGCATGT TAGCCAGTCC CAGACAGCAT CGCAGGCTTC TGTGGTGGTG 
AAATCAATCC CTACCTCCTC CAGTGGAGCC ATCACTCATA TCATGCAACA GGCACTGAGC 
AGTCACACAG CTTTCACCAA GAGAAGCGAG GAGACTGTGA CTGAAGAAGG GGAGGTGGAG 
GAAATGGACA CTTTAGACCC CCAAACGGGC ATCCTGTACC GGGCCGCCAT GGCACAAAAG 
CAGCAGAAAC AGGTTCATCT GCAGCAGGAA GGAGCTCATC TGCAAGTGAA GACGGTGCAG 
TCCCTGCAGA CCAAGCAGAA ACAGATCATT CAGCTCCAGC CGGATCAAAT GCAGCACAAA 
ATCCAACAGA CGGCGCAGCT CTCCATCCGC CAGCAAAAGG TCACTCCCAT GCAGCAGGAG 
CACGCACAGC GCCACCTGGT GGTGAAAGAG AGACACATCC CTAACATTAT GTCTCAGCCC 
CAGCAGACTG TAGTGCAAGT GCTGGCCGTT AAAACCACCC AGCAGCTGCC CAAACTGCAG 
CAGGTTCAAG GTCAACAAAA GATTTACATG CAGACTCCAA TACAAATGCC AGTCTCATCT 
GAGAAACAAG CTGTTACGCA GATGACCCAG TCTATCGTGT CCCCAGGATC AGCCGTTACA 
AAGATCACCT TTGAGGGCCA CCAACCTCCC ACCGTCTCCA AGGCATCTGT AAGCGACTGC 
CTGCCCAGAC TGACCCCCGC AGTCGCCAGC ATCCTGCCTA CTGTCCAGAG CTCTGAGAAG 
TCCTCCGTAG CCGATATCCT GAAGATGTCT ATGATGGAGG CAGACATTGA CACGAATGCC 
GAGCCCATGG TTGTTGATGC CCCCACCAAA GTTGCCCCCA TTATTAAACC CATGCCCATG 
CTGACTGAAG CTACAGTAAC CCAGGTTCCA AGGTTTTCTG TGCCAGTGAC TGCTTCTGTT 
CCACCGCCTC CAAAATGTTT CCCGGCCCCC ATTATAACGG CCGCGCCCCC TACTGTGCTG 
CCTGGACCTC TCCCACCGCC ACCCCCTCTT ACAAGGAAAG TCGACGTCCC CATTGCCAGC 
CAGATCATCC AGATACAGAA CGTTCAGCAA AAGAGAACTG AGGAAGTGCC TTCTGAAATA 
ATTATCCAGA CCATCCCTCA GTATCATGCC ATCCCTTGTC ACTCCAGCTC CAATGTGGTA 
GTGGAACCCA GTGGGCTCCT GGAGATGAGT AACTTTACCA GCCAGAGGCT CGGGGAGGAA 
GACGTCGTCT TGGCTCACGA CGGGGAGAGT GGCAAAAGCC AAGCCCCTAT CCATATCGAG 
GACGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912277.1
CDS	439..4287
Translation	MIGPSAGGRALCGGEARMPVVWPTLLDFSRDECKRILRKLELEAYAGVISALRAQGDLCKEKKDLLGELSKVLSISTERHRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRLVPLMPRLVPQTAFTATANAVANAAGQHNASLPDPAETGNKEVVVCYSYTSTTSTPTSTPVPSGSVATVKSPRPASPASNVVVLPSGSTVYVKSVNCSDDDEKPRKRRRTNSNSSSPVVLKEVPKPTTPVSKTITVPVTGSPKMSNIMQSIANSLPPHMSPVKITFTKPATQTTNTSTQKVIIVTTSPSSTFVPNILSKSHNYAAVTKLVPTSVITSATQKQPLVVTASQSSVVTSSSTSSSSPPLTSGSIAVTTVVSSTPSLVMSTVAPGASTSAVKAATNRLPSPKSLVTSPSQILAQLPKQYQQSPKHQIHTIPQQTQQLAQATAITNPGTITHPPTAQQPPMPAAMKPTIQIKQESGVKIITQQVQPSKILPKPVSTSLPNSSNAPIMVVSSNGAIMTTKLVTTPSGTQATYTRPTISSALGARMAATPGATYVKTTSGSIITVVPKSLATLGGKIISSNIVSGTTTKITTIPMTSKPNVIVVQKTTGKGTTIQGLPGKNVVTTLLNAGGDKTLQAMPAGTKPAIITATRPITKMIVTQPKGMGSTVQPATKIIPTKIVYGQQGKTQVLIKPKPVTFQATVMSEQTRQLVTETLQQASRVVEATSATIQEVKEDTESSSSSTESSQSSQDSQPVVHVIASRSQDWAEHEIAMDTTPTIIYQDIGGESQSATSTIKALMELQQTAGKRQMASTNTVSSGDMPLPSLLQVSHPSQQLQQVQPTRTLLQHVSQSQTASQASVVVKSIPTSSSGAITHIMQQALSSHTAFTKRSEETVTEEGEVEEMDTLDPQTGILYRAAMAQKQQKQVHLQQEGAHLQVKTVQSLQTKQKQIIQLQPDQMQHKIQQTAQLSIRQQKVTPMQQEHAQRHLVVKERHIPNIMSQPQQTVVQVLAVKTTQQLPKLQQVQGQQKIYMQTPIQMPVSSEKQAVTQMTQSIVSPGSAVTKITFEGHQPPTVSKASVSDCLPRLTPAVASILPTVQSSEKSSVADILKMSMMEADIDTNAEPMVVDAPTKVAPIIKPMPMLTEATVTQVPRFSVPVTASVPPPPKCFPAPIITAAPPTVLPGPLPPPPPLTRKVDVPIASQIIQIQNVQQKRTEEVPSEIIIQTIPQYHAIPCHSSSNVVVEPSGLLEMSNFTSQRLGEEDVVLAHDGESGKSQAPIHIEDV

Target ORF information:

RefSeq Version	XM_004912220.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912220.3

ORF Insert Sequence:

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAACTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG GCAAAAGACA AATGGCTTCT ACTAACACCG TGAGCTCTGG GGATATGCCT 
CTCCCATCCC TACTGCAAGT GAGCCATCCA TCGCAGCAGC TGCAGCAGGT CCAACCCACG 
CGCACCCTAT TGCAGCATGT TAGCCAGTCC CAGACAGCAT CGCAGGCTTC TGTGGTGGTG 
AAATCAATCC CTACCTCCTC CAGTGGAGCC ATCACTCATA TCATGCAACA GGCACTGAGC 
AGTCACACAG CTTTCACCAA GAGAAGCGAG GAGACTGTGA CTGAAGAAGG GGAGGTGGAG 
GAAATGGACA CTTTAGACCC CCAAACGGGC ATCCTGTACC GGGCCGCCAT GGCACAAAAG 
CAGCAGAAAC AGGTTCATCT GCAGCAGGAA GGAGCTCATC TGCAAGTGAA GACGGTGCAG 
TCCCTGCAGA CCAAGCAGAA ACAGATCATT CAGCTCCAGC CGGATCAAAT GCAGCACAAA 
ATCCAACAGA CGGCGCAGCT CTCCATCCGC CAGCAAAAGG TCACTCCCAT GCAGCAGGAG 
CACGCACAGC GCCACCTGGT GGTGAAAGAG AGACACATCC CTAACATTAT GTCTCAGCCC 
CAGCAGACTG TAGTGCAAGT GCTGGCCGTT AAAACCACCC AGCAGCTGCC CAAACTGCAG 
CAGGTTCAAG GTCAACAAAA GATTTACATG CAGACTCCAA TACAAATGCC AGTCTCATCT 
GAGAAACAAG CTGTTACGCA GATGACCCAG TCTATCGTGT CCCCAGGATC AGCCGTTACA 
AAGATCACCT TTGAGGGCCA CCAACCTCCC ACCGTCTCCA AGGCATCTGT AAGCGACTGC 
CTGCCCAGAC TGACCCCCGC AGTCGCCAGC ATCCTGCCTA CTGTCCAGAG CTCTGAGAAG 
TCCTCCGTAG CCGATATCCT GAAGATGTCT ATGATGGAGG CAGACATTGA CACGAATGCC 
GAGCCCATGG TTGTTGATGC CCCCACCAAA GTTGCCCCCA TTATTAAACC CATGCCCATG 
CTGACTGAAG CTACAGTAAC CCAGGTTCCA AGGTTTTCTG TGCCAGTGAC TGCTTCTGTT 
CCACCGCCTC CAAAATGTTT CCCGGCCCCC ATTATAACGG CCGCGCCCCC TACTGTGCTG 
CCTGGACCTC TCCCACCGCC ACCCCCTCTT ACAAGGAAAG TCGACGTCCC CATTGCCAGC 
CAGATCATCC AGATACAGAA CGTTCAGCAA AAGAGAACTG AGGAAGTGCC TTCTGAAATA 
ATTATCCAGA CCATCCCTCA GTATCATGCC ATCCCTTGTC ACTCCAGCTC CAATGTGGTA 
GTGGAACCCA GTGGGCTCCT GGAGATGAGT AACTTTACCA GCCAGAGGCT CGGGGAGGAA 
GACGTCGTCT TGGCTCACGA CGGGGAGAGT GGCAAAAGCC AAGCCCCTAT CCATATCGAG 
GACGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emsy ( XM_004912217.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912274.1 Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa06522
Clone ID Related Accession (Same CDS sequence)	XM_004912217.3
Accession Version	XM_004912217.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	BRCA2-interacting transcriptional repressor EMSY isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912217.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAACTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG TGAAAGAGAA AGTGGAAAAT AAACTACGAC AGCCGACTAT CGATCTAAGT 
CAAATGGCTG TTCCCATTCA GATGGCTGCA GATAAACGCC ACGCTGAAGG CAGCTCTATT 
GCCATGGTGG AGTCAGAGCT GGTGGCAGAA TACCTAAGTA CAGGCAAAAG ACAAATGGCT 
TCTACTAACA CCGTGAGCTC TGGGGATATG CCTCTCCCAT CCCTACTGCA AGTGAGCCAT 
CCATCGCAGC AGCTGCAGCA GGTCCAACCC ACGCGCACCC TATTGCAGCA TGTTAGCCAG 
TCCCAGACAG CATCGCAGGC TTCTGTGGTG GTGAAATCAA TCCCTACCTC CTCCAGTGGA 
GCCATCACTC ATATCATGCA ACAGCATTTT ACTAACTTTG TCTCACAGGC ACTGAGCAGT 
CACACAGCTT TCACCAAGAG AAGCGAGGAG ACTGTGACTG AAGAAGGGGA GGTGGAGGAA 
ATGGACACTT TAGACCCCCA AACGGGCATC CTGTACCGGG CCGCCATGGC ACAAAAGCAG 
CAGAAACAGG TTCATCTGCA GCAGGAAGGA GCTCATCTGC AAGTGAAGAC GGTGCAGTCC 
CTGCAGACCA AGCAGAAACA GATCATTCAG CTCCAGCCGG ATCAAATGCA GCACAAAATC 
CAACAGACGG CGCAGCTCTC CATCCGCCAG CAAAAGGTCA CTCCCATGCA GCAGGAGCAC 
GCACAGCGCC ACCTGGTGGT GAAAGAGAGA CACATCCCTA ACATTATGTC TCAGCCCCAG 
CAGACTGTAG TGCAAGTGCT GGCCGTTAAA ACCACCCAGC AGCTGCCCAA ACTGCAGCAG 
GTTCAAGGTC AACAAAAGAT TTACATGCAG ACTCCAATAC AAATGCCAGT CTCATCTGAG 
AAACAAGCTG TTACGCAGAT GACCCAGTCT ATCGTGTCCC CAGGATCAGC CGTTACAAAG 
ATCACCTTTG AGGGCCACCA ACCTCCCACC GTCTCCAAGG CATCTGTAAG CGACTGCCTG 
CCCAGACTGA CCCCCGCAGT CGCCAGCATC CTGCCTACTG TCCAGAGCTC TGAGAAGTCC 
TCCGTAGCCG ATATCCTGAA GATGTCTATG ATGGAGGCAG ACATTGACAC GAATGCCGAG 
CCCATGGTTG TTGATGCCCC CACCAAAGTT GCCCCCATTA TTAAACCCAT GCCCATGCTG 
ACTGAAGCTA CAGTAACCCA GGTTCCAAGG TTTTCTGTGC CAGTGACTGC TTCTGTTCCA 
CCGCCTCCAA AATGTTTCCC GGCCCCCATT ATAACGGCCG CGCCCCCTAC TGTGCTGCCT 
GGACCTCTCC CACCGCCACC CCCTCTTACA AGGAAAGTCG ACGTCCCCAT TGCCAGCCAG 
ATCATCCAGA TACAGAACGT TCAGCAAAAG AGAACTGAGG AAGTGCCTTC TGAAATAATT 
ATCCAGACCA TCCCTCAGTA TCATGCCATC CCTTGTCACT CCAGCTCCAA TGTGGTAGTG 
GAACCCAGTG GGCTCCTGGA GATGAGTAAC TTTACCAGCC AGAGGCTCGG GGAGGAAGAC 
GTCGTCTTGG CTCACGACGG GGAGAGTGGC AAAAGCCAAG CCCCTATCCA TATCGAGGAC 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912274.1
CDS	440..4465
Translation	MIGPSAGGRALCGGEARMPVVWPTLLDFSRDECKRILRKLELEAYAGVISALRAQGDLCKEKKDLLGELSKVLSISTERHRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRLVPLMPRLVPQTAFTATANAVANAAGQHNASLPDPAETGNKEVVVCYSYTSTTSTPTSTPVPSGSVATVKSPRPASPASNVVVLPSGSTVYVKSVNCSDDDEKPRKRRRTNSNSSSPVVLKEVPKPTTPVSKTITVPVTGSPKMSNIMQSIANSLPPHMSPVKITFTKPATQTTNTSTQKVIIVTTSPSSTFVPNILSKSHNYAAVTKLVPTSVITSATQKQPLVVTASQSSVVTSSSTSSSSPPLTSGSIAVTTVVSSTPSLVMSTVAPGASTSAVKAATNRLPSPKSLVTSPSQILAQLPKQYQQSPKHQIHTIPQQTQQLAQATAITNPGTITHPPTAQQPPMPAAMKPTIQIKQESGVKIITQQVQPSKILPKPVSTSLPNSSNAPIMVVSSNGAIMTTKLVTTPSGTQATYTRPTISSALGARMAATPGATYVKTTSGSIITVVPKSLATLGGKIISSNIVSGTTTKITTIPMTSKPNVIVVQKTTGKGTTIQGLPGKNVVTTLLNAGGDKTLQAMPAGTKPAIITATRPITKMIVTQPKGMGSTVQPATKIIPTKIVYGQQGKTQVLIKPKPVTFQATVMSEQTRQLVTETLQQASRVVEATSATIQEVKEDTESSSSSTESSQSSQDSQPVVHVIASRSQDWAEHEIAMDTTPTIIYQDIGGESQSATSTIKALMELQQTAVKEKVENKLRQPTIDLSQMAVPIQMAADKRHAEGSSIAMVESELVAEYLSTGKRQMASTNTVSSGDMPLPSLLQVSHPSQQLQQVQPTRTLLQHVSQSQTASQASVVVKSIPTSSSGAITHIMQQHFTNFVSQALSSHTAFTKRSEETVTEEGEVEEMDTLDPQTGILYRAAMAQKQQKQVHLQQEGAHLQVKTVQSLQTKQKQIIQLQPDQMQHKIQQTAQLSIRQQKVTPMQQEHAQRHLVVKERHIPNIMSQPQQTVVQVLAVKTTQQLPKLQQVQGQQKIYMQTPIQMPVSSEKQAVTQMTQSIVSPGSAVTKITFEGHQPPTVSKASVSDCLPRLTPAVASILPTVQSSEKSSVADILKMSMMEADIDTNAEPMVVDAPTKVAPIIKPMPMLTEATVTQVPRFSVPVTASVPPPPKCFPAPIITAAPPTVLPGPLPPPPPLTRKVDVPIASQIIQIQNVQQKRTEEVPSEIIIQTIPQYHAIPCHSSSNVVVEPSGLLEMSNFTSQRLGEEDVVLAHDGESGKSQAPIHIEDV

Target ORF information:

RefSeq Version	XM_004912217.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912217.3

ORF Insert Sequence:

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAACTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG TGAAAGAGAA AGTGGAAAAT AAACTACGAC AGCCGACTAT CGATCTAAGT 
CAAATGGCTG TTCCCATTCA GATGGCTGCA GATAAACGCC ACGCTGAAGG CAGCTCTATT 
GCCATGGTGG AGTCAGAGCT GGTGGCAGAA TACCTAAGTA CAGGCAAAAG ACAAATGGCT 
TCTACTAACA CCGTGAGCTC TGGGGATATG CCTCTCCCAT CCCTACTGCA AGTGAGCCAT 
CCATCGCAGC AGCTGCAGCA GGTCCAACCC ACGCGCACCC TATTGCAGCA TGTTAGCCAG 
TCCCAGACAG CATCGCAGGC TTCTGTGGTG GTGAAATCAA TCCCTACCTC CTCCAGTGGA 
GCCATCACTC ATATCATGCA ACAGCATTTT ACTAACTTTG TCTCACAGGC ACTGAGCAGT 
CACACAGCTT TCACCAAGAG AAGCGAGGAG ACTGTGACTG AAGAAGGGGA GGTGGAGGAA 
ATGGACACTT TAGACCCCCA AACGGGCATC CTGTACCGGG CCGCCATGGC ACAAAAGCAG 
CAGAAACAGG TTCATCTGCA GCAGGAAGGA GCTCATCTGC AAGTGAAGAC GGTGCAGTCC 
CTGCAGACCA AGCAGAAACA GATCATTCAG CTCCAGCCGG ATCAAATGCA GCACAAAATC 
CAACAGACGG CGCAGCTCTC CATCCGCCAG CAAAAGGTCA CTCCCATGCA GCAGGAGCAC 
GCACAGCGCC ACCTGGTGGT GAAAGAGAGA CACATCCCTA ACATTATGTC TCAGCCCCAG 
CAGACTGTAG TGCAAGTGCT GGCCGTTAAA ACCACCCAGC AGCTGCCCAA ACTGCAGCAG 
GTTCAAGGTC AACAAAAGAT TTACATGCAG ACTCCAATAC AAATGCCAGT CTCATCTGAG 
AAACAAGCTG TTACGCAGAT GACCCAGTCT ATCGTGTCCC CAGGATCAGC CGTTACAAAG 
ATCACCTTTG AGGGCCACCA ACCTCCCACC GTCTCCAAGG CATCTGTAAG CGACTGCCTG 
CCCAGACTGA CCCCCGCAGT CGCCAGCATC CTGCCTACTG TCCAGAGCTC TGAGAAGTCC 
TCCGTAGCCG ATATCCTGAA GATGTCTATG ATGGAGGCAG ACATTGACAC GAATGCCGAG 
CCCATGGTTG TTGATGCCCC CACCAAAGTT GCCCCCATTA TTAAACCCAT GCCCATGCTG 
ACTGAAGCTA CAGTAACCCA GGTTCCAAGG TTTTCTGTGC CAGTGACTGC TTCTGTTCCA 
CCGCCTCCAA AATGTTTCCC GGCCCCCATT ATAACGGCCG CGCCCCCTAC TGTGCTGCCT 
GGACCTCTCC CACCGCCACC CCCTCTTACA AGGAAAGTCG ACGTCCCCAT TGCCAGCCAG 
ATCATCCAGA TACAGAACGT TCAGCAAAAG AGAACTGAGG AAGTGCCTTC TGAAATAATT 
ATCCAGACCA TCCCTCAGTA TCATGCCATC CCTTGTCACT CCAGCTCCAA TGTGGTAGTG 
GAACCCAGTG GGCTCCTGGA GATGAGTAAC TTTACCAGCC AGAGGCTCGG GGAGGAAGAC 
GTCGTCTTGG CTCACGACGG GGAGAGTGGC AAAAGCCAAG CCCCTATCCA TATCGAGGAC 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emsy ( XM_004912218.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912275.1 Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa06523
Clone ID Related Accession (Same CDS sequence)	XM_004912218.3
Accession Version	XM_004912218.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	BRCA2-interacting transcriptional repressor EMSY isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912218.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAACTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG TGAAAGAGAA AGTGGAAAAT AAACTACGAC AGCCGACTAT CGATCTAAGT 
CAAATGGCTG TTCCCATTCA GATGGCTGCA GATAAACGCC ACGCTGAAGG CAGCTCTATT 
GCCATGGTGG AGTCAGAGCT GGTGGCAGAA TACCTAAGTA CAGGCAAAAG ACAAATGGCT 
TCTACTAACA CCGTGAGCTC TGGGGATATG CCTCTCCCAT CCCTACTGCA AGTGAGCCAT 
CCATCGCAGC AGCTGCAGCA GGTCCAACCC ACGCGCACCC TATTGCAGCA TGTTAGCCAG 
TCCCAGACAG CATCGCAGGC TTCTGTGGTG GTGAAATCAA TCCCTACCTC CTCCAGTGGA 
GCCATCACTC ATATCATGCA ACAGGCACTG AGCAGTCACA CAGCTTTCAC CAAGAGAAGC 
GAGGAGACTG TGACTGAAGA AGGGGAGGTG GAGGAAATGG ACACTTTAGA CCCCCAAACG 
GGCATCCTGT ACCGGGCCGC CATGGCACAA AAGCAGCAGA AACAGGTTCA TCTGCAGCAG 
GAAGGAGCTC ATCTGCAAGT GAAGACGGTG CAGTCCCTGC AGACCAAGCA GAAACAGATC 
ATTCAGCTCC AGCCGGATCA AATGCAGCAC AAAATCCAAC AGACGGCGCA GCTCTCCATC 
CGCCAGCAAA AGGTCACTCC CATGCAGCAG GAGCACGCAC AGCGCCACCT GGTGGTGAAA 
GAGAGACACA TCCCTAACAT TATGTCTCAG CCCCAGCAGA CTGTAGTGCA AGTGCTGGCC 
GTTAAAACCA CCCAGCAGCT GCCCAAACTG CAGCAGGTTC AAGGTCAACA AAAGATTTAC 
ATGCAGACTC CAATACAAAT GCCAGTCTCA TCTGAGAAAC AAGCTGTTAC GCAGATGACC 
CAGTCTATCG TGTCCCCAGG ATCAGCCGTT ACAAAGATCA CCTTTGAGGG CCACCAACCT 
CCCACCGTCT CCAAGGCATC TGTAAGCGAC TGCCTGCCCA GACTGACCCC CGCAGTCGCC 
AGCATCCTGC CTACTGTCCA GAGCTCTGAG AAGTCCTCCG TAGCCGATAT CCTGAAGATG 
TCTATGATGG AGGCAGACAT TGACACGAAT GCCGAGCCCA TGGTTGTTGA TGCCCCCACC 
AAAGTTGCCC CCATTATTAA ACCCATGCCC ATGCTGACTG AAGCTACAGT AACCCAGGTT 
CCAAGGTTTT CTGTGCCAGT GACTGCTTCT GTTCCACCGC CTCCAAAATG TTTCCCGGCC 
CCCATTATAA CGGCCGCGCC CCCTACTGTG CTGCCTGGAC CTCTCCCACC GCCACCCCCT 
CTTACAAGGA AAGTCGACGT CCCCATTGCC AGCCAGATCA TCCAGATACA GAACGTTCAG 
CAAAAGAGAA CTGAGGAAGT GCCTTCTGAA ATAATTATCC AGACCATCCC TCAGTATCAT 
GCCATCCCTT GTCACTCCAG CTCCAATGTG GTAGTGGAAC CCAGTGGGCT CCTGGAGATG 
AGTAACTTTA CCAGCCAGAG GCTCGGGGAG GAAGACGTCG TCTTGGCTCA CGACGGGGAG 
AGTGGCAAAA GCCAAGCCCC TATCCATATC GAGGACGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912275.1
CDS	439..4440
Translation	MIGPSAGGRALCGGEARMPVVWPTLLDFSRDECKRILRKLELEAYAGVISALRAQGDLCKEKKDLLGELSKVLSISTERHRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRLVPLMPRLVPQTAFTATANAVANAAGQHNASLPDPAETGNKEVVVCYSYTSTTSTPTSTPVPSGSVATVKSPRPASPASNVVVLPSGSTVYVKSVNCSDDDEKPRKRRRTNSNSSSPVVLKEVPKPTTPVSKTITVPVTGSPKMSNIMQSIANSLPPHMSPVKITFTKPATQTTNTSTQKVIIVTTSPSSTFVPNILSKSHNYAAVTKLVPTSVITSATQKQPLVVTASQSSVVTSSSTSSSSPPLTSGSIAVTTVVSSTPSLVMSTVAPGASTSAVKAATNRLPSPKSLVTSPSQILAQLPKQYQQSPKHQIHTIPQQTQQLAQATAITNPGTITHPPTAQQPPMPAAMKPTIQIKQESGVKIITQQVQPSKILPKPVSTSLPNSSNAPIMVVSSNGAIMTTKLVTTPSGTQATYTRPTISSALGARMAATPGATYVKTTSGSIITVVPKSLATLGGKIISSNIVSGTTTKITTIPMTSKPNVIVVQKTTGKGTTIQGLPGKNVVTTLLNAGGDKTLQAMPAGTKPAIITATRPITKMIVTQPKGMGSTVQPATKIIPTKIVYGQQGKTQVLIKPKPVTFQATVMSEQTRQLVTETLQQASRVVEATSATIQEVKEDTESSSSSTESSQSSQDSQPVVHVIASRSQDWAEHEIAMDTTPTIIYQDIGGESQSATSTIKALMELQQTAVKEKVENKLRQPTIDLSQMAVPIQMAADKRHAEGSSIAMVESELVAEYLSTGKRQMASTNTVSSGDMPLPSLLQVSHPSQQLQQVQPTRTLLQHVSQSQTASQASVVVKSIPTSSSGAITHIMQQALSSHTAFTKRSEETVTEEGEVEEMDTLDPQTGILYRAAMAQKQQKQVHLQQEGAHLQVKTVQSLQTKQKQIIQLQPDQMQHKIQQTAQLSIRQQKVTPMQQEHAQRHLVVKERHIPNIMSQPQQTVVQVLAVKTTQQLPKLQQVQGQQKIYMQTPIQMPVSSEKQAVTQMTQSIVSPGSAVTKITFEGHQPPTVSKASVSDCLPRLTPAVASILPTVQSSEKSSVADILKMSMMEADIDTNAEPMVVDAPTKVAPIIKPMPMLTEATVTQVPRFSVPVTASVPPPPKCFPAPIITAAPPTVLPGPLPPPPPLTRKVDVPIASQIIQIQNVQQKRTEEVPSEIIIQTIPQYHAIPCHSSSNVVVEPSGLLEMSNFTSQRLGEEDVVLAHDGESGKSQAPIHIEDV

Target ORF information:

RefSeq Version	XM_004912218.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis EMSY, BRCA2-interacting transcriptional repressor (emsy), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912218.3

ORF Insert Sequence:

ATGATCGGCC CTTCGGCGGG AGGGAGGGCG CTCTGTGGAG GAGAGGCCAG GATGCCGGTG 
GTGTGGCCCA CACTCCTGGA CTTCAGCAGG GATGAGTGCA AGAGGATTCT TCGCAAGTTG 
GAATTGGAGG CATACGCAGG CGTTATTAGT GCCCTTCGGG CACAGGGAGA CCTCTGCAAG 
GAGAAGAAGG ACCTTCTAGG AGAACTTTCC AAAGTCTTAA GCATTTCAAC AGAGCGGCAT 
CGTGCTGAGG TTCGAAGAGC TGTCAACGAT GAGCGACTTA CAACAATTGC CCACAACATG 
TCCGGTCCAA ATAGCTCTTC CGAGTGGTCA ATTGAGGGGC GGCGCTTGGT ACCACTGATG 
CCGCGCTTAG TCCCTCAAAC GGCTTTCACA GCCACCGCCA ACGCAGTGGC AAATGCCGCC 
GGGCAGCACA ACGCCTCGCT CCCTGACCCT GCAGAGACTG GCAACAAAGA AGTGGTGGTT 
TGTTACTCCT ATACGAGTAC TACATCAACA CCGACCTCCA CTCCAGTCCC TAGCGGAAGC 
GTAGCCACCG TGAAGTCTCC TCGGCCTGCA AGTCCTGCGT CAAATGTCGT CGTCCTACCC 
AGCGGCAGCA CCGTCTATGT CAAAAGTGTC AACTGTTCGG ACGATGACGA AAAGCCACGT 
AAAAGGAGGC GCACAAATTC CAACAGCTCT TCTCCGGTGG TACTGAAGGA GGTTCCCAAA 
CCCACAACGC CAGTGTCCAA AACAATCACG GTGCCTGTGA CCGGCAGCCC CAAGATGAGC 
AACATCATGC AGAGCATTGC CAACTCCTTG CCCCCCCACA TGTCTCCTGT GAAGATCACC 
TTCACCAAGC CTGCCACCCA GACCACAAAC ACTTCTACCC AAAAGGTGAT AATAGTTACC 
ACCTCTCCGA GTTCAACATT TGTTCCCAAC ATATTATCCA AGTCCCACAA TTATGCTGCA 
GTCACCAAAT TGGTCCCCAC CTCAGTCATT ACCTCTGCAA CACAAAAACA GCCGCTGGTA 
GTCACTGCCT CTCAGTCCTC TGTAGTCACA AGCAGCAGTA CAAGTTCATC TTCTCCACCA 
CTGACCTCCG GTAGCATTGC TGTTACTACA GTGGTATCCT CTACTCCATC TCTGGTCATG 
TCAACCGTAG CACCAGGTGC TTCAACATCT GCAGTCAAGG CAGCTACCAA CAGACTCCCA 
TCCCCCAAGA GCCTGGTCAC CTCCCCTAGT CAGATCCTGG CCCAGTTACC AAAACAGTAC 
CAGCAGTCCC CCAAGCACCA GATACATACG ATACCCCAGC AGACGCAGCA GCTGGCCCAG 
GCCACAGCAA TCACAAACCC AGGCACCATC ACTCACCCAC CCACTGCACA GCAGCCTCCA 
ATGCCTGCTG CCATGAAACC AACCATACAG ATCAAGCAGG AATCGGGTGT TAAGATCATC 
ACTCAGCAAG TGCAGCCCAG TAAGATCTTG CCCAAACCAG TGTCCACATC CCTGCCGAAC 
AGCAGCAACG CTCCCATTAT GGTGGTGAGC AGCAATGGTG CAATCATGAC TACAAAATTG 
GTCACCACCC CGAGTGGGAC ACAAGCCACG TACACACGTC CAACCATCAG CTCTGCCTTG 
GGAGCCCGCA TGGCTGCTAC ACCCGGGGCT ACCTATGTAA AAACCACAAG CGGGAGCATA 
ATCACCGTGG TACCGAAATC GCTGGCTACA CTGGGAGGGA AAATCATAAG CAGCAACATA 
GTGTCAGGTA CAACAACAAA GATCACTACA ATTCCAATGA CCTCCAAGCC TAATGTGATT 
GTGGTGCAGA AAACTACAGG CAAAGGAACG ACGATACAAG GCCTTCCGGG CAAAAACGTT 
GTGACCACAT TACTGAATGC CGGGGGGGAT AAAACCCTAC AAGCGATGCC AGCAGGAACA 
AAGCCAGCTA TAATCACGGC AACTCGCCCA ATCACCAAGA TGATTGTGAC GCAGCCCAAG 
GGTATGGGAT CGACTGTTCA GCCAGCAACC AAAATCATCC CCACCAAAAT AGTGTATGGG 
CAACAGGGGA AGACCCAGGT CCTTATAAAG CCCAAGCCAG TGACGTTCCA GGCCACTGTG 
ATGAGCGAAC AGACCAGGCA GCTGGTGACA GAAACCCTGC AGCAAGCCTC CCGTGTGGTA 
GAGGCAACAA GTGCTACTAT TCAAGAGGTC AAAGAGGATA CAGAGAGCAG CTCCTCTTCC 
ACGGAGTCCT CCCAGAGCTC TCAAGATTCC CAGCCTGTTG TTCATGTTAT TGCTTCTCGA 
AGCCAGGATT GGGCAGAACA TGAAATAGCC ATGGATACCA CTCCTACCAT CATCTACCAG 
GATATTGGGG GAGAATCCCA GTCTGCTACA TCGACCATTA AGGCCCTTAT GGAGCTCCAG 
CAGACTGCAG TGAAAGAGAA AGTGGAAAAT AAACTACGAC AGCCGACTAT CGATCTAAGT 
CAAATGGCTG TTCCCATTCA GATGGCTGCA GATAAACGCC ACGCTGAAGG CAGCTCTATT 
GCCATGGTGG AGTCAGAGCT GGTGGCAGAA TACCTAAGTA CAGGCAAAAG ACAAATGGCT 
TCTACTAACA CCGTGAGCTC TGGGGATATG CCTCTCCCAT CCCTACTGCA AGTGAGCCAT 
CCATCGCAGC AGCTGCAGCA GGTCCAACCC ACGCGCACCC TATTGCAGCA TGTTAGCCAG 
TCCCAGACAG CATCGCAGGC TTCTGTGGTG GTGAAATCAA TCCCTACCTC CTCCAGTGGA 
GCCATCACTC ATATCATGCA ACAGGCACTG AGCAGTCACA CAGCTTTCAC CAAGAGAAGC 
GAGGAGACTG TGACTGAAGA AGGGGAGGTG GAGGAAATGG ACACTTTAGA CCCCCAAACG 
GGCATCCTGT ACCGGGCCGC CATGGCACAA AAGCAGCAGA AACAGGTTCA TCTGCAGCAG 
GAAGGAGCTC ATCTGCAAGT GAAGACGGTG CAGTCCCTGC AGACCAAGCA GAAACAGATC 
ATTCAGCTCC AGCCGGATCA AATGCAGCAC AAAATCCAAC AGACGGCGCA GCTCTCCATC 
CGCCAGCAAA AGGTCACTCC CATGCAGCAG GAGCACGCAC AGCGCCACCT GGTGGTGAAA 
GAGAGACACA TCCCTAACAT TATGTCTCAG CCCCAGCAGA CTGTAGTGCA AGTGCTGGCC 
GTTAAAACCA CCCAGCAGCT GCCCAAACTG CAGCAGGTTC AAGGTCAACA AAAGATTTAC 
ATGCAGACTC CAATACAAAT GCCAGTCTCA TCTGAGAAAC AAGCTGTTAC GCAGATGACC 
CAGTCTATCG TGTCCCCAGG ATCAGCCGTT ACAAAGATCA CCTTTGAGGG CCACCAACCT 
CCCACCGTCT CCAAGGCATC TGTAAGCGAC TGCCTGCCCA GACTGACCCC CGCAGTCGCC 
AGCATCCTGC CTACTGTCCA GAGCTCTGAG AAGTCCTCCG TAGCCGATAT CCTGAAGATG 
TCTATGATGG AGGCAGACAT TGACACGAAT GCCGAGCCCA TGGTTGTTGA TGCCCCCACC 
AAAGTTGCCC CCATTATTAA ACCCATGCCC ATGCTGACTG AAGCTACAGT AACCCAGGTT 
CCAAGGTTTT CTGTGCCAGT GACTGCTTCT GTTCCACCGC CTCCAAAATG TTTCCCGGCC 
CCCATTATAA CGGCCGCGCC CCCTACTGTG CTGCCTGGAC CTCTCCCACC GCCACCCCCT 
CTTACAAGGA AAGTCGACGT CCCCATTGCC AGCCAGATCA TCCAGATACA GAACGTTCAG 
CAAAAGAGAA CTGAGGAAGT GCCTTCTGAA ATAATTATCC AGACCATCCC TCAGTATCAT 
GCCATCCCTT GTCACTCCAG CTCCAATGTG GTAGTGGAAC CCAGTGGGCT CCTGGAGATG 
AGTAACTTTA CCAGCCAGAG GCTCGGGGAG GAAGACGTCG TCTTGGCTCA CGACGGGGAG 
AGTGGCAAAA GCCAAGCCCC TATCCATATC GAGGACGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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emsy cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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