aak1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012965973.2	XP_012821427.1	AP2-associated protein kinase 1 isoform X2
XM_012965975.2	XP_012821429.1	AP2-associated protein kinase 1 isoform X4
XM_012965983.2	XP_012821437.1	AP2-associated protein kinase 1 isoform X9
XM_012965972.2	XP_012821426.1	AP2-associated protein kinase 1 isoform X1
XM_012965974.2	XP_012821428.1	AP2-associated protein kinase 1 isoform X3
XM_031897602.1	XP_031753462.1	AP2-associated protein kinase 1 isoform X4
XM_012965981.3	XP_012821435.1	AP2-associated protein kinase 1 isoform X7
XM_012965985.3	XP_012821439.1	AP2-associated protein kinase 1 isoform X11
XM_012965973.3	XP_012821427.1	AP2-associated protein kinase 1 isoform X2
NM_001114046.1	NP_001107518.1	AP2-associated protein kinase 1
XM_012965978.2	XP_012821432.1	AP2-associated protein kinase 1 isoform X5
XM_012965982.3	XP_012821436.1	AP2-associated protein kinase 1 isoform X8
XM_012965980.3	XP_012821434.1	AP2-associated protein kinase 1 isoform X6
XM_012965980.2	XP_012821434.1	AP2-associated protein kinase 1 isoform X6
XM_012965981.2	XP_012821435.1	AP2-associated protein kinase 1 isoform X7
XM_012965985.2	XP_012821439.1	AP2-associated protein kinase 1 isoform X11
XM_012965978.3	XP_012821432.1	AP2-associated protein kinase 1 isoform X5
XM_012965982.2	XP_012821436.1	AP2-associated protein kinase 1 isoform X8
XM_012965984.2	XP_012821438.1	AP2-associated protein kinase 1 isoform X10
XM_012965972.3	XP_012821426.1	AP2-associated protein kinase 1 isoform X1
XM_012965974.3	XP_012821428.1	AP2-associated protein kinase 1 isoform X3
XM_012965983.3	XP_012821437.1	AP2-associated protein kinase 1 isoform X9
XM_012965984.3	XP_012821438.1	AP2-associated protein kinase 1 isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa31265	XM_012965983.3 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa31266	XM_012965984.3 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa31267	XM_012965985.3 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa31255	XM_012965972.3 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa31256	XM_012965973.3 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa31257	XM_012965974.3 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa31259	XM_031897602.1 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa31260	XM_012965978.3 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa31262	XM_012965980.3 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa31263	XM_012965981.3 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa31264	XM_012965982.3 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa25095	NM_001114046.1 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OXa31258	XM_012965975.2 Latest version!	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
OXa31256	XM_012965973.2	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OXa31262	XM_012965980.2	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OXa31263	XM_012965981.2	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OXa31267	XM_012965985.2	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OXa31260	XM_012965978.2	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OXa31264	XM_012965982.2	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OXa31255	XM_012965972.2	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OXa31257	XM_012965974.2	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OXa31265	XM_012965983.2	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa31266	XM_012965984.2	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

aak1 ( XM_012965983.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821437.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OXa31265
Clone ID Related Accession (Same CDS sequence)	XM_012965983.2 , XM_012965983.3
Accession Version	XM_012965983.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965983.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG GAAATCAGAG 
CTGGAGGTAG AAAGCCTCAT TCCTGGGCTG GAGCCCCCAA TGCCCCAGCG ACTTTCATCC 
CAGGCAGACT CTATTGCTTC AAACCGAACA GACTCCCTCA CTGGGGAAGA TTCTCTCCTT 
GACTACTCTC TTCTCTCTAA CCCCGCGGCT GATCTTCTTG ACGAGTTTGC TCCTGTAACA 
CATTCGGCTC TGGCTTCCAC AGACGATACT AACCTGCTAA CGGGCTTTGA TATCCCGGAC 
GATACAGAAA AAAGGGCTGA TGATGAATTT GATCCTATTC CAGTTCTGGT ATCCAAAAAT 
TCCCAAGGTA CTCACTCTAG TAGCAGCAGT GGGAATTCTG AGTCGAGCCT ACCAAACATG 
ACCATGTCTC TTCTACTGGT GGATCAGCTC ATAGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821437.1
CDS	609..3410
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGKSELEVESLIPGLEPPMPQRLSSQADSIASNRTDSLTGEDSLLDYSLLSNPAADLLDEFAPVTHSALASTDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGTHSSSSSGNSESSLPNMTMSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_012965983.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965983.3

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG GAAATCAGAG 
CTGGAGGTAG AAAGCCTCAT TCCTGGGCTG GAGCCCCCAA TGCCCCAGCG ACTTTCATCC 
CAGGCAGACT CTATTGCTTC AAACCGAACA GACTCCCTCA CTGGGGAAGA TTCTCTCCTT 
GACTACTCTC TTCTCTCTAA CCCCGCGGCT GATCTTCTTG ACGAGTTTGC TCCTGTAACA 
CATTCGGCTC TGGCTTCCAC AGACGATACT AACCTGCTAA CGGGCTTTGA TATCCCGGAC 
GATACAGAAA AAAGGGCTGA TGATGAATTT GATCCTATTC CAGTTCTGGT ATCCAAAAAT 
TCCCAAGGTA CTCACTCTAG TAGCAGCAGT GGGAATTCTG AGTCGAGCCT ACCAAACATG 
ACCATGTCTC TTCTACTGGT GGATCAGCTC ATAGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965984.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821438.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OXa31266
Clone ID Related Accession (Same CDS sequence)	XM_012965984.2 , XM_012965984.3
Accession Version	XM_012965984.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965984.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG TACTCACTCT 
AGTAGCAGCA GTGGGAATTC TGAGTCGAGC CTACCAAACA TGACCATGTC TCTTCTACTG 
GTGGATCAGC TCATAGACTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821438.1
CDS	605..3088
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGTHSSSSSGNSESSLPNMTMSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_012965984.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965984.3

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG TACTCACTCT 
AGTAGCAGCA GTGGGAATTC TGAGTCGAGC CTACCAAACA TGACCATGTC TCTTCTACTG 
GTGGATCAGC TCATAGACTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965985.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821439.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OXa31267
Clone ID Related Accession (Same CDS sequence)	XM_012965985.3 , XM_012965985.2
Accession Version	XM_012965985.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965985.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGGT ACTCACTCTA GTAGCAGCAG TGGGAATTCT 
GAGTCGAGCC TACCAAACAT GACCATGTCT CTTCTACTGG TGGATCAGCT CATAGACTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821439.1
CDS	602..2884
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSGTHSSSSSGNSESSLPNMTMSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_012965985.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965985.3

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGGT ACTCACTCTA GTAGCAGCAG TGGGAATTCT 
GAGTCGAGCC TACCAAACAT GACCATGTCT CTTCTACTGG TGGATCAGCT CATAGACTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965972.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821426.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31255
Clone ID Related Accession (Same CDS sequence)	XM_012965972.2 , XM_012965972.3
Accession Version	XM_012965972.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965972.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG GAAATCAGAG 
CTGGAGGTAG AAAGCCTCAT TCCTGGGCTG GAGCCCCCAA TGCCCCAGCG ACTTTCATCC 
CAGGCAGACT CTATTGCTTC AAACCGAACA GACTCCCTCA CTGGGGAAGA TTCTCTCCTT 
GACTACTCTC TTCTCTCTAA CCCCGCGGCT GATCTTCTTG ACGAGTTTGC TCCTGTAACA 
CATTCGGCTC TGGCTTCCAC AGACGATACT AACCTGCTAA CGGGCTTTGA TATCCCGGAC 
GATACAGAAA AAAGGGCTGA TGATGAATTT GATCCTATTC CAGTTCTGGT ATCCAAAAAT 
TCCCAAGGTG TGCATCAAGA TATGAAGGTA TTTCCTCCTG GAAGCGATGG GCATATTAAA 
ATGCACTCAG GTGGAGACTA TCCTCTGGCT GCTGACAACA AACACTTCCT TGAAATGAAA 
AATGAGTGTC CATCAAAGGG TCCATCTAGC TGCAGTTCTT TTCATAGCAG TGATGGAGAA 
GCAACTGATC ATGACCCTGA CTTGCTCGAC TGCAGTGGAT CAAGACCTTT ACTAATGGAA 
TCAGATGATG ATGAATGTTC TAGGAAAACT AAGGATTTAG AAGTTCCAAG TCCTCCTATA 
GATGAAATTA GCACCCAGAA TTCTCAACTT CCTTTTCACA ATGTAAACTC TTGGCGGCCA 
GAGTTGAGCT TGGCTGAAGG TTTTGATGTA TTCTCCACTG CTCCATTCAA GGGTCCTAAA 
TTAACACAGG ATGAATCAGA AATATTTACC AATGCTCCCT TTGTAGCCAA GACTGCATTC 
CCTTCATTCC AGTCACAGGA ACCTGATGTG TTCCTCAGGG CCCCTTTTTC CAGGAAACTC 
AGTACCGAAG AAGCATCTCA AAATATTTCA TCCCAATCAT TTTCGTCTCT ACTTGGGCTT 
GATGTGGTAG GTAATTCTAC ATTCCGAAAT TCTGAAATGG CAGTTGGATC CTCTTCAATG 
AAGACCTCGT ACACTACAGG CAATCTCTAT TCGCATTCCA TCAGACCCTT AGAATCAGCT 
GACTCCTCTA TTTTTACTAA TACCTCAATG GAACCAGGCA TTATAGAGAA TCACATGCTT 
CCAGGGCACG AGGCTGCTCA AGAGACAGTA TTTGGCACGG TGGTCACAAA GCCTTTTCGC 
CCACAGTCAT TGTCAAAGTA TTCCAGGCAC TATAGCCCTC AAGATGGGCG GAATGTGGAT 
GCTCAACCAA TTGCTGCTTT CAAAGTCGTC TCCAAGAGCG CAAACACCTC TGTAGCTGGC 
TCTGTTCCTA TATCCTCTGT GACTTACAGG ACCTCAGAAA TCACTAATGT AGACCCATTT 
GGCTCTGCTC CTTTTCCTGC TAAAGCTAGC AGGCCAAAGC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821426.1
CDS	612..4376
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGKSELEVESLIPGLEPPMPQRLSSQADSIASNRTDSLTGEDSLLDYSLLSNPAADLLDEFAPVTHSALASTDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965972.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965972.3

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG GAAATCAGAG 
CTGGAGGTAG AAAGCCTCAT TCCTGGGCTG GAGCCCCCAA TGCCCCAGCG ACTTTCATCC 
CAGGCAGACT CTATTGCTTC AAACCGAACA GACTCCCTCA CTGGGGAAGA TTCTCTCCTT 
GACTACTCTC TTCTCTCTAA CCCCGCGGCT GATCTTCTTG ACGAGTTTGC TCCTGTAACA 
CATTCGGCTC TGGCTTCCAC AGACGATACT AACCTGCTAA CGGGCTTTGA TATCCCGGAC 
GATACAGAAA AAAGGGCTGA TGATGAATTT GATCCTATTC CAGTTCTGGT ATCCAAAAAT 
TCCCAAGGTG TGCATCAAGA TATGAAGGTA TTTCCTCCTG GAAGCGATGG GCATATTAAA 
ATGCACTCAG GTGGAGACTA TCCTCTGGCT GCTGACAACA AACACTTCCT TGAAATGAAA 
AATGAGTGTC CATCAAAGGG TCCATCTAGC TGCAGTTCTT TTCATAGCAG TGATGGAGAA 
GCAACTGATC ATGACCCTGA CTTGCTCGAC TGCAGTGGAT CAAGACCTTT ACTAATGGAA 
TCAGATGATG ATGAATGTTC TAGGAAAACT AAGGATTTAG AAGTTCCAAG TCCTCCTATA 
GATGAAATTA GCACCCAGAA TTCTCAACTT CCTTTTCACA ATGTAAACTC TTGGCGGCCA 
GAGTTGAGCT TGGCTGAAGG TTTTGATGTA TTCTCCACTG CTCCATTCAA GGGTCCTAAA 
TTAACACAGG ATGAATCAGA AATATTTACC AATGCTCCCT TTGTAGCCAA GACTGCATTC 
CCTTCATTCC AGTCACAGGA ACCTGATGTG TTCCTCAGGG CCCCTTTTTC CAGGAAACTC 
AGTACCGAAG AAGCATCTCA AAATATTTCA TCCCAATCAT TTTCGTCTCT ACTTGGGCTT 
GATGTGGTAG GTAATTCTAC ATTCCGAAAT TCTGAAATGG CAGTTGGATC CTCTTCAATG 
AAGACCTCGT ACACTACAGG CAATCTCTAT TCGCATTCCA TCAGACCCTT AGAATCAGCT 
GACTCCTCTA TTTTTACTAA TACCTCAATG GAACCAGGCA TTATAGAGAA TCACATGCTT 
CCAGGGCACG AGGCTGCTCA AGAGACAGTA TTTGGCACGG TGGTCACAAA GCCTTTTCGC 
CCACAGTCAT TGTCAAAGTA TTCCAGGCAC TATAGCCCTC AAGATGGGCG GAATGTGGAT 
GCTCAACCAA TTGCTGCTTT CAAAGTCGTC TCCAAGAGCG CAAACACCTC TGTAGCTGGC 
TCTGTTCCTA TATCCTCTGT GACTTACAGG ACCTCAGAAA TCACTAATGT AGACCCATTT 
GGCTCTGCTC CTTTTCCTGC TAAAGCTAGC AGGCCAAAGC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965973.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821427.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X2, mRNA.

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CloneID	OXa31256
Clone ID Related Accession (Same CDS sequence)	XM_012965973.2 , XM_012965973.3
Accession Version	XM_012965973.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965973.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCCAGC TCCACTCAAG 
ACCCCACAGA AGCCCCAGCC ACCTACAGCA CCTCCATCTT CTGCACCTCC CACAGCAGCA 
GGGCAGAAAC TGGGATCCCT TACCCCTCCT TCATCCCCCA AGACCCAAAG AGGCGGCCAC 
AGAAGGATAC TTAGTGATGT TACGCATAGT GCAGTTTTCG GTGTCCCAGC CAGTAAATCT 
ACTCAGCTTC TACAGGCTGC TGCGGCTGAA GCAAACCTCA ACAAATCAAA GTCAGCTACA 
ACCACCCCTT CTGGCTCACC TCGGACCTCC CAGCAGAATG TTCACAATCC GCCAGAACCT 
TCTGGCTGGA ATCCTTTCGA CGATGACAAC TTTTCCAAAC TCACAGCTGA GGAACTTCTA 
AATAAGGATT TTGCCAAGCT TGGAGAAAGT AAGCCATCGG AGCAGCTGAG CCGCTCAGCA 
GAAGATCTCC TATCTGGGTT TCCAGCTCCA CCTGCTGTAT CTCAGGCTGA TTCATTTGGA 
ACTGCACTCT TTGCCAGTGG TTCAGCTGCA GATAAACCTG TAGATTCACA GGGGCCCAGT 
CCTGGCTCTC AGCTGCAAAA AACTTCTGAT TCTTTCTTGC CTCTCCCTCT GTCTGACACC 
CCAGAAAAAT TGATTGAGGG TCTCAAATCT CCAGAGCCCC AGCTTCTCCT CCCTCATCTT 
GTGCAGCTGG CAGATCCCTT TGGGAGCACA GCAGAGGATG GAAATGGGAA ATCAGAGCTG 
GAGGTAGAAA GCCTCATTCC TGGGCTGGAG CCCCCAATGC CCCAGCGACT TTCATCCCAG 
GCAGACTCTA TTGCTTCAAA CCGAACAGAC TCCCTCACTG GGGAAGATTC TCTCCTTGAC 
TACTCTCTTC TCTCTAACCC CGCGGCTGAT CTTCTTGACG AGTTTGCTCC TGTAACACAT 
TCGGCTCTGG CTTCCACAGA CGATACTAAC CTGCTAACGG GCTTTGATAT CCCGGACGAT 
ACAGAAAAAA GGGCTGATGA TGAATTTGAT CCTATTCCAG TTCTGGTATC CAAAAATTCC 
CAAGGTGTGC ATCAAGATAT GAAGGTATTT CCTCCTGGAA GCGATGGGCA TATTAAAATG 
CACTCAGGTG GAGACTATCC TCTGGCTGCT GACAACAAAC ACTTCCTTGA AATGAAAAAT 
GAGTGTCCAT CAAAGGGTCC ATCTAGCTGC AGTTCTTTTC ATAGCAGTGA TGGAGAAGCA 
ACTGATCATG ACCCTGACTT GCTCGACTGC AGTGGATCAA GACCTTTACT AATGGAATCA 
GATGATGATG AATGTTCTAG GAAAACTAAG GATTTAGAAG TTCCAAGTCC TCCTATAGAT 
GAAATTAGCA CCCAGAATTC TCAACTTCCT TTTCACAATG TAAACTCTTG GCGGCCAGAG 
TTGAGCTTGG CTGAAGGTTT TGATGTATTC TCCACTGCTC CATTCAAGGG TCCTAAATTA 
ACACAGGATG AATCAGAAAT ATTTACCAAT GCTCCCTTTG TAGCCAAGAC TGCATTCCCT 
TCATTCCAGT CACAGGAACC TGATGTGTTC CTCAGGGCCC CTTTTTCCAG GAAACTCAGT 
ACCGAAGAAG CATCTCAAAA TATTTCATCC CAATCATTTT CGTCTCTACT TGGGCTTGAT 
GTGGTAGGTA ATTCTACATT CCGAAATTCT GAAATGGCAG TTGGATCCTC TTCAATGAAG 
ACCTCGTACA CTACAGGCAA TCTCTATTCG CATTCCATCA GACCCTTAGA ATCAGCTGAC 
TCCTCTATTT TTACTAATAC CTCAATGGAA CCAGGCATTA TAGAGAATCA CATGCTTCCA 
GGGCACGAGG CTGCTCAAGA GACAGTATTT GGCACGGTGG TCACAAAGCC TTTTCGCCCA 
CAGTCATTGT CAAAGTATTC CAGGCACTAT AGCCCTCAAG ATGGGCGGAA TGTGGATGCT 
CAACCAATTG CTGCTTTCAA AGTCGTCTCC AAGAGCGCAA ACACCTCTGT AGCTGGCTCT 
GTTCCTATAT CCTCTGTGAC TTACAGGACC TCAGAAATCA CTAATGTAGA CCCATTTGGC 
TCTGCTCCTT TTCCTGCTAA AGCTAGCAGG CCAAAGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821427.1
CDS	614..4375
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGKSELEVESLIPGLEPPMPQRLSSQADSIASNRTDSLTGEDSLLDYSLLSNPAADLLDEFAPVTHSALASTDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965973.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965973.3

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCCAGC TCCACTCAAG 
ACCCCACAGA AGCCCCAGCC ACCTACAGCA CCTCCATCTT CTGCACCTCC CACAGCAGCA 
GGGCAGAAAC TGGGATCCCT TACCCCTCCT TCATCCCCCA AGACCCAAAG AGGCGGCCAC 
AGAAGGATAC TTAGTGATGT TACGCATAGT GCAGTTTTCG GTGTCCCAGC CAGTAAATCT 
ACTCAGCTTC TACAGGCTGC TGCGGCTGAA GCAAACCTCA ACAAATCAAA GTCAGCTACA 
ACCACCCCTT CTGGCTCACC TCGGACCTCC CAGCAGAATG TTCACAATCC GCCAGAACCT 
TCTGGCTGGA ATCCTTTCGA CGATGACAAC TTTTCCAAAC TCACAGCTGA GGAACTTCTA 
AATAAGGATT TTGCCAAGCT TGGAGAAAGT AAGCCATCGG AGCAGCTGAG CCGCTCAGCA 
GAAGATCTCC TATCTGGGTT TCCAGCTCCA CCTGCTGTAT CTCAGGCTGA TTCATTTGGA 
ACTGCACTCT TTGCCAGTGG TTCAGCTGCA GATAAACCTG TAGATTCACA GGGGCCCAGT 
CCTGGCTCTC AGCTGCAAAA AACTTCTGAT TCTTTCTTGC CTCTCCCTCT GTCTGACACC 
CCAGAAAAAT TGATTGAGGG TCTCAAATCT CCAGAGCCCC AGCTTCTCCT CCCTCATCTT 
GTGCAGCTGG CAGATCCCTT TGGGAGCACA GCAGAGGATG GAAATGGGAA ATCAGAGCTG 
GAGGTAGAAA GCCTCATTCC TGGGCTGGAG CCCCCAATGC CCCAGCGACT TTCATCCCAG 
GCAGACTCTA TTGCTTCAAA CCGAACAGAC TCCCTCACTG GGGAAGATTC TCTCCTTGAC 
TACTCTCTTC TCTCTAACCC CGCGGCTGAT CTTCTTGACG AGTTTGCTCC TGTAACACAT 
TCGGCTCTGG CTTCCACAGA CGATACTAAC CTGCTAACGG GCTTTGATAT CCCGGACGAT 
ACAGAAAAAA GGGCTGATGA TGAATTTGAT CCTATTCCAG TTCTGGTATC CAAAAATTCC 
CAAGGTGTGC ATCAAGATAT GAAGGTATTT CCTCCTGGAA GCGATGGGCA TATTAAAATG 
CACTCAGGTG GAGACTATCC TCTGGCTGCT GACAACAAAC ACTTCCTTGA AATGAAAAAT 
GAGTGTCCAT CAAAGGGTCC ATCTAGCTGC AGTTCTTTTC ATAGCAGTGA TGGAGAAGCA 
ACTGATCATG ACCCTGACTT GCTCGACTGC AGTGGATCAA GACCTTTACT AATGGAATCA 
GATGATGATG AATGTTCTAG GAAAACTAAG GATTTAGAAG TTCCAAGTCC TCCTATAGAT 
GAAATTAGCA CCCAGAATTC TCAACTTCCT TTTCACAATG TAAACTCTTG GCGGCCAGAG 
TTGAGCTTGG CTGAAGGTTT TGATGTATTC TCCACTGCTC CATTCAAGGG TCCTAAATTA 
ACACAGGATG AATCAGAAAT ATTTACCAAT GCTCCCTTTG TAGCCAAGAC TGCATTCCCT 
TCATTCCAGT CACAGGAACC TGATGTGTTC CTCAGGGCCC CTTTTTCCAG GAAACTCAGT 
ACCGAAGAAG CATCTCAAAA TATTTCATCC CAATCATTTT CGTCTCTACT TGGGCTTGAT 
GTGGTAGGTA ATTCTACATT CCGAAATTCT GAAATGGCAG TTGGATCCTC TTCAATGAAG 
ACCTCGTACA CTACAGGCAA TCTCTATTCG CATTCCATCA GACCCTTAGA ATCAGCTGAC 
TCCTCTATTT TTACTAATAC CTCAATGGAA CCAGGCATTA TAGAGAATCA CATGCTTCCA 
GGGCACGAGG CTGCTCAAGA GACAGTATTT GGCACGGTGG TCACAAAGCC TTTTCGCCCA 
CAGTCATTGT CAAAGTATTC CAGGCACTAT AGCCCTCAAG ATGGGCGGAA TGTGGATGCT 
CAACCAATTG CTGCTTTCAA AGTCGTCTCC AAGAGCGCAA ACACCTCTGT AGCTGGCTCT 
GTTCCTATAT CCTCTGTGAC TTACAGGACC TCAGAAATCA CTAATGTAGA CCCATTTGGC 
TCTGCTCCTT TTCCTGCTAA AGCTAGCAGG CCAAAGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965974.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821428.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa31257
Clone ID Related Accession (Same CDS sequence)	XM_012965974.2 , XM_012965974.3
Accession Version	XM_012965974.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965974.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGAA AAATTGATTG AGGGTCTCAA ATCTCCAGAG 
CCCCAGCTTC TCCTCCCTCA TCTTGTGCAG CTGGCAGATC CCTTTGGGAG CACAGCAGAG 
GATGGAAATG GGAAATCAGA GCTGGAGGTA GAAAGCCTCA TTCCTGGGCT GGAGCCCCCA 
ATGCCCCAGC GACTTTCATC CCAGGCAGAC TCTATTGCTT CAAACCGAAC AGACTCCCTC 
ACTGGGGAAG ATTCTCTCCT TGACTACTCT CTTCTCTCTA ACCCCGCGGC TGATCTTCTT 
GACGAGTTTG CTCCTGTAAC ACATTCGGCT CTGGCTTCCA CAGACGATAC TAACCTGCTA 
ACGGGCTTTG ATATCCCGGA CGATACAGAA AAAAGGGCTG ATGATGAATT TGATCCTATT 
CCAGTTCTGG TATCCAAAAA TTCCCAAGGT GTGCATCAAG ATATGAAGGT ATTTCCTCCT 
GGAAGCGATG GGCATATTAA AATGCACTCA GGTGGAGACT ATCCTCTGGC TGCTGACAAC 
AAACACTTCC TTGAAATGAA AAATGAGTGT CCATCAAAGG GTCCATCTAG CTGCAGTTCT 
TTTCATAGCA GTGATGGAGA AGCAACTGAT CATGACCCTG ACTTGCTCGA CTGCAGTGGA 
TCAAGACCTT TACTAATGGA ATCAGATGAT GATGAATGTT CTAGGAAAAC TAAGGATTTA 
GAAGTTCCAA GTCCTCCTAT AGATGAAATT AGCACCCAGA ATTCTCAACT TCCTTTTCAC 
AATGTAAACT CTTGGCGGCC AGAGTTGAGC TTGGCTGAAG GTTTTGATGT ATTCTCCACT 
GCTCCATTCA AGGGTCCTAA ATTAACACAG GATGAATCAG AAATATTTAC CAATGCTCCC 
TTTGTAGCCA AGACTGCATT CCCTTCATTC CAGTCACAGG AACCTGATGT GTTCCTCAGG 
GCCCCTTTTT CCAGGAAACT CAGTACCGAA GAAGCATCTC AAAATATTTC ATCCCAATCA 
TTTTCGTCTC TACTTGGGCT TGATGTGGTA GGTAATTCTA CATTCCGAAA TTCTGAAATG 
GCAGTTGGAT CCTCTTCAAT GAAGACCTCG TACACTACAG GCAATCTCTA TTCGCATTCC 
ATCAGACCCT TAGAATCAGC TGACTCCTCT ATTTTTACTA ATACCTCAAT GGAACCAGGC 
ATTATAGAGA ATCACATGCT TCCAGGGCAC GAGGCTGCTC AAGAGACAGT ATTTGGCACG 
GTGGTCACAA AGCCTTTTCG CCCACAGTCA TTGTCAAAGT ATTCCAGGCA CTATAGCCCT 
CAAGATGGGC GGAATGTGGA TGCTCAACCA ATTGCTGCTT TCAAAGTCGT CTCCAAGAGC 
GCAAACACCT CTGTAGCTGG CTCTGTTCCT ATATCCTCTG TGACTTACAG GACCTCAGAA 
ATCACTAATG TAGACCCATT TGGCTCTGCT CCTTTTCCTG CTAAAGCTAG CAGGCCAAAG 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821428.1
CDS	611..4276
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGKSELEVESLIPGLEPPMPQRLSSQADSIASNRTDSLTGEDSLLDYSLLSNPAADLLDEFAPVTHSALASTDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965974.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965974.3

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGAA AAATTGATTG AGGGTCTCAA ATCTCCAGAG 
CCCCAGCTTC TCCTCCCTCA TCTTGTGCAG CTGGCAGATC CCTTTGGGAG CACAGCAGAG 
GATGGAAATG GGAAATCAGA GCTGGAGGTA GAAAGCCTCA TTCCTGGGCT GGAGCCCCCA 
ATGCCCCAGC GACTTTCATC CCAGGCAGAC TCTATTGCTT CAAACCGAAC AGACTCCCTC 
ACTGGGGAAG ATTCTCTCCT TGACTACTCT CTTCTCTCTA ACCCCGCGGC TGATCTTCTT 
GACGAGTTTG CTCCTGTAAC ACATTCGGCT CTGGCTTCCA CAGACGATAC TAACCTGCTA 
ACGGGCTTTG ATATCCCGGA CGATACAGAA AAAAGGGCTG ATGATGAATT TGATCCTATT 
CCAGTTCTGG TATCCAAAAA TTCCCAAGGT GTGCATCAAG ATATGAAGGT ATTTCCTCCT 
GGAAGCGATG GGCATATTAA AATGCACTCA GGTGGAGACT ATCCTCTGGC TGCTGACAAC 
AAACACTTCC TTGAAATGAA AAATGAGTGT CCATCAAAGG GTCCATCTAG CTGCAGTTCT 
TTTCATAGCA GTGATGGAGA AGCAACTGAT CATGACCCTG ACTTGCTCGA CTGCAGTGGA 
TCAAGACCTT TACTAATGGA ATCAGATGAT GATGAATGTT CTAGGAAAAC TAAGGATTTA 
GAAGTTCCAA GTCCTCCTAT AGATGAAATT AGCACCCAGA ATTCTCAACT TCCTTTTCAC 
AATGTAAACT CTTGGCGGCC AGAGTTGAGC TTGGCTGAAG GTTTTGATGT ATTCTCCACT 
GCTCCATTCA AGGGTCCTAA ATTAACACAG GATGAATCAG AAATATTTAC CAATGCTCCC 
TTTGTAGCCA AGACTGCATT CCCTTCATTC CAGTCACAGG AACCTGATGT GTTCCTCAGG 
GCCCCTTTTT CCAGGAAACT CAGTACCGAA GAAGCATCTC AAAATATTTC ATCCCAATCA 
TTTTCGTCTC TACTTGGGCT TGATGTGGTA GGTAATTCTA CATTCCGAAA TTCTGAAATG 
GCAGTTGGAT CCTCTTCAAT GAAGACCTCG TACACTACAG GCAATCTCTA TTCGCATTCC 
ATCAGACCCT TAGAATCAGC TGACTCCTCT ATTTTTACTA ATACCTCAAT GGAACCAGGC 
ATTATAGAGA ATCACATGCT TCCAGGGCAC GAGGCTGCTC AAGAGACAGT ATTTGGCACG 
GTGGTCACAA AGCCTTTTCG CCCACAGTCA TTGTCAAAGT ATTCCAGGCA CTATAGCCCT 
CAAGATGGGC GGAATGTGGA TGCTCAACCA ATTGCTGCTT TCAAAGTCGT CTCCAAGAGC 
GCAAACACCT CTGTAGCTGG CTCTGTTCCT ATATCCTCTG TGACTTACAG GACCTCAGAA 
ATCACTAATG TAGACCCATT TGGCTCTGCT CCTTTTCCTG CTAAAGCTAG CAGGCCAAAG 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_031897602.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753462.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa31259
Clone ID Related Accession (Same CDS sequence)	XM_031897602.1
Accession Version	XM_031897602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGGG AAATCAGAGC TGGAGGTAGA AAGCCTCATT 
CCTGGGCTGG AGCCCCCAAT GCCCCAGCGA CTTTCATCCC AGGCAGACTC TATTGCTTCA 
AACCGAACAG ACTCCCTCAC TGGGGAAGAT TCTCTCCTTG ACTACTCTCT TCTCTCTAAC 
CCCGCGGCTG ATCTTCTTGA CGAGTTTGCT CCTGTAACAC ATTCGGCTCT GGCTTCCACA 
GACGATACTA ACCTGCTAAC GGGCTTTGAT ATCCCGGACG ATACAGAAAA AAGGGCTGAT 
GATGAATTTG ATCCTATTCC AGTTCTGGTA TCCAAAAATT CCCAAGGTGT GCATCAAGAT 
ATGAAGGTAT TTCCTCCTGG AAGCGATGGG CATATTAAAA TGCACTCAGG TGGAGACTAT 
CCTCTGGCTG CTGACAACAA ACACTTCCTT GAAATGAAAA ATGAGTGTCC ATCAAAGGGT 
CCATCTAGCT GCAGTTCTTT TCATAGCAGT GATGGAGAAG CAACTGATCA TGACCCTGAC 
TTGCTCGACT GCAGTGGATC AAGACCTTTA CTAATGGAAT CAGATGATGA TGAATGTTCT 
AGGAAAACTA AGGATTTAGA AGTTCCAAGT CCTCCTATAG ATGAAATTAG CACCCAGAAT 
TCTCAACTTC CTTTTCACAA TGTAAACTCT TGGCGGCCAG AGTTGAGCTT GGCTGAAGGT 
TTTGATGTAT TCTCCACTGC TCCATTCAAG GGTCCTAAAT TAACACAGGA TGAATCAGAA 
ATATTTACCA ATGCTCCCTT TGTAGCCAAG ACTGCATTCC CTTCATTCCA GTCACAGGAA 
CCTGATGTGT TCCTCAGGGC CCCTTTTTCC AGGAAACTCA GTACCGAAGA AGCATCTCAA 
AATATTTCAT CCCAATCATT TTCGTCTCTA CTTGGGCTTG ATGTGGTAGG TAATTCTACA 
TTCCGAAATT CTGAAATGGC AGTTGGATCC TCTTCAATGA AGACCTCGTA CACTACAGGC 
AATCTCTATT CGCATTCCAT CAGACCCTTA GAATCAGCTG ACTCCTCTAT TTTTACTAAT 
ACCTCAATGG AACCAGGCAT TATAGAGAAT CACATGCTTC CAGGGCACGA GGCTGCTCAA 
GAGACAGTAT TTGGCACGGT GGTCACAAAG CCTTTTCGCC CACAGTCATT GTCAAAGTAT 
TCCAGGCACT ATAGCCCTCA AGATGGGCGG AATGTGGATG CTCAACCAAT TGCTGCTTTC 
AAAGTCGTCT CCAAGAGCGC AAACACCTCT GTAGCTGGCT CTGTTCCTAT ATCCTCTGTG 
ACTTACAGGA CCTCAGAAAT CACTAATGTA GACCCATTTG GCTCTGCTCC TTTTCCTGCT 
AAAGCTAGCA GGCCAAAGCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753462.1
CDS	611..4174
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSGKSELEVESLIPGLEPPMPQRLSSQADSIASNRTDSLTGEDSLLDYSLLSNPAADLLDEFAPVTHSALASTDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_031897602.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897602.1

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGGG AAATCAGAGC TGGAGGTAGA AAGCCTCATT 
CCTGGGCTGG AGCCCCCAAT GCCCCAGCGA CTTTCATCCC AGGCAGACTC TATTGCTTCA 
AACCGAACAG ACTCCCTCAC TGGGGAAGAT TCTCTCCTTG ACTACTCTCT TCTCTCTAAC 
CCCGCGGCTG ATCTTCTTGA CGAGTTTGCT CCTGTAACAC ATTCGGCTCT GGCTTCCACA 
GACGATACTA ACCTGCTAAC GGGCTTTGAT ATCCCGGACG ATACAGAAAA AAGGGCTGAT 
GATGAATTTG ATCCTATTCC AGTTCTGGTA TCCAAAAATT CCCAAGGTGT GCATCAAGAT 
ATGAAGGTAT TTCCTCCTGG AAGCGATGGG CATATTAAAA TGCACTCAGG TGGAGACTAT 
CCTCTGGCTG CTGACAACAA ACACTTCCTT GAAATGAAAA ATGAGTGTCC ATCAAAGGGT 
CCATCTAGCT GCAGTTCTTT TCATAGCAGT GATGGAGAAG CAACTGATCA TGACCCTGAC 
TTGCTCGACT GCAGTGGATC AAGACCTTTA CTAATGGAAT CAGATGATGA TGAATGTTCT 
AGGAAAACTA AGGATTTAGA AGTTCCAAGT CCTCCTATAG ATGAAATTAG CACCCAGAAT 
TCTCAACTTC CTTTTCACAA TGTAAACTCT TGGCGGCCAG AGTTGAGCTT GGCTGAAGGT 
TTTGATGTAT TCTCCACTGC TCCATTCAAG GGTCCTAAAT TAACACAGGA TGAATCAGAA 
ATATTTACCA ATGCTCCCTT TGTAGCCAAG ACTGCATTCC CTTCATTCCA GTCACAGGAA 
CCTGATGTGT TCCTCAGGGC CCCTTTTTCC AGGAAACTCA GTACCGAAGA AGCATCTCAA 
AATATTTCAT CCCAATCATT TTCGTCTCTA CTTGGGCTTG ATGTGGTAGG TAATTCTACA 
TTCCGAAATT CTGAAATGGC AGTTGGATCC TCTTCAATGA AGACCTCGTA CACTACAGGC 
AATCTCTATT CGCATTCCAT CAGACCCTTA GAATCAGCTG ACTCCTCTAT TTTTACTAAT 
ACCTCAATGG AACCAGGCAT TATAGAGAAT CACATGCTTC CAGGGCACGA GGCTGCTCAA 
GAGACAGTAT TTGGCACGGT GGTCACAAAG CCTTTTCGCC CACAGTCATT GTCAAAGTAT 
TCCAGGCACT ATAGCCCTCA AGATGGGCGG AATGTGGATG CTCAACCAAT TGCTGCTTTC 
AAAGTCGTCT CCAAGAGCGC AAACACCTCT GTAGCTGGCT CTGTTCCTAT ATCCTCTGTG 
ACTTACAGGA CCTCAGAAAT CACTAATGTA GACCCATTTG GCTCTGCTCC TTTTCCTGCT 
AAAGCTAGCA GGCCAAAGCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965978.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821432.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X5, mRNA.

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CloneID	OXa31260
Clone ID Related Accession (Same CDS sequence)	XM_012965978.2 , XM_012965978.3
Accession Version	XM_012965978.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965978.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGA CGATACTAAC 
CTGCTAACGG GCTTTGATAT CCCGGACGAT ACAGAAAAAA GGGCTGATGA TGAATTTGAT 
CCTATTCCAG TTCTGGTATC CAAAAATTCC CAAGGTGTGC ATCAAGATAT GAAGGTATTT 
CCTCCTGGAA GCGATGGGCA TATTAAAATG CACTCAGGTG GAGACTATCC TCTGGCTGCT 
GACAACAAAC ACTTCCTTGA AATGAAAAAT GAGTGTCCAT CAAAGGGTCC ATCTAGCTGC 
AGTTCTTTTC ATAGCAGTGA TGGAGAAGCA ACTGATCATG ACCCTGACTT GCTCGACTGC 
AGTGGATCAA GACCTTTACT AATGGAATCA GATGATGATG AATGTTCTAG GAAAACTAAG 
GATTTAGAAG TTCCAAGTCC TCCTATAGAT GAAATTAGCA CCCAGAATTC TCAACTTCCT 
TTTCACAATG TAAACTCTTG GCGGCCAGAG TTGAGCTTGG CTGAAGGTTT TGATGTATTC 
TCCACTGCTC CATTCAAGGG TCCTAAATTA ACACAGGATG AATCAGAAAT ATTTACCAAT 
GCTCCCTTTG TAGCCAAGAC TGCATTCCCT TCATTCCAGT CACAGGAACC TGATGTGTTC 
CTCAGGGCCC CTTTTTCCAG GAAACTCAGT ACCGAAGAAG CATCTCAAAA TATTTCATCC 
CAATCATTTT CGTCTCTACT TGGGCTTGAT GTGGTAGGTA ATTCTACATT CCGAAATTCT 
GAAATGGCAG TTGGATCCTC TTCAATGAAG ACCTCGTACA CTACAGGCAA TCTCTATTCG 
CATTCCATCA GACCCTTAGA ATCAGCTGAC TCCTCTATTT TTACTAATAC CTCAATGGAA 
CCAGGCATTA TAGAGAATCA CATGCTTCCA GGGCACGAGG CTGCTCAAGA GACAGTATTT 
GGCACGGTGG TCACAAAGCC TTTTCGCCCA CAGTCATTGT CAAAGTATTC CAGGCACTAT 
AGCCCTCAAG ATGGGCGGAA TGTGGATGCT CAACCAATTG CTGCTTTCAA AGTCGTCTCC 
AAGAGCGCAA ACACCTCTGT AGCTGGCTCT GTTCCTATAT CCTCTGTGAC TTACAGGACC 
TCAGAAATCA CTAATGTAGA CCCATTTGGC TCTGCTCCTT TTCCTGCTAA AGCTAGCAGG 
CCAAAGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821432.1
CDS	610..4161
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965978.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965978.3

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGA CGATACTAAC 
CTGCTAACGG GCTTTGATAT CCCGGACGAT ACAGAAAAAA GGGCTGATGA TGAATTTGAT 
CCTATTCCAG TTCTGGTATC CAAAAATTCC CAAGGTGTGC ATCAAGATAT GAAGGTATTT 
CCTCCTGGAA GCGATGGGCA TATTAAAATG CACTCAGGTG GAGACTATCC TCTGGCTGCT 
GACAACAAAC ACTTCCTTGA AATGAAAAAT GAGTGTCCAT CAAAGGGTCC ATCTAGCTGC 
AGTTCTTTTC ATAGCAGTGA TGGAGAAGCA ACTGATCATG ACCCTGACTT GCTCGACTGC 
AGTGGATCAA GACCTTTACT AATGGAATCA GATGATGATG AATGTTCTAG GAAAACTAAG 
GATTTAGAAG TTCCAAGTCC TCCTATAGAT GAAATTAGCA CCCAGAATTC TCAACTTCCT 
TTTCACAATG TAAACTCTTG GCGGCCAGAG TTGAGCTTGG CTGAAGGTTT TGATGTATTC 
TCCACTGCTC CATTCAAGGG TCCTAAATTA ACACAGGATG AATCAGAAAT ATTTACCAAT 
GCTCCCTTTG TAGCCAAGAC TGCATTCCCT TCATTCCAGT CACAGGAACC TGATGTGTTC 
CTCAGGGCCC CTTTTTCCAG GAAACTCAGT ACCGAAGAAG CATCTCAAAA TATTTCATCC 
CAATCATTTT CGTCTCTACT TGGGCTTGAT GTGGTAGGTA ATTCTACATT CCGAAATTCT 
GAAATGGCAG TTGGATCCTC TTCAATGAAG ACCTCGTACA CTACAGGCAA TCTCTATTCG 
CATTCCATCA GACCCTTAGA ATCAGCTGAC TCCTCTATTT TTACTAATAC CTCAATGGAA 
CCAGGCATTA TAGAGAATCA CATGCTTCCA GGGCACGAGG CTGCTCAAGA GACAGTATTT 
GGCACGGTGG TCACAAAGCC TTTTCGCCCA CAGTCATTGT CAAAGTATTC CAGGCACTAT 
AGCCCTCAAG ATGGGCGGAA TGTGGATGCT CAACCAATTG CTGCTTTCAA AGTCGTCTCC 
AAGAGCGCAA ACACCTCTGT AGCTGGCTCT GTTCCTATAT CCTCTGTGAC TTACAGGACC 
TCAGAAATCA CTAATGTAGA CCCATTTGGC TCTGCTCCTT TTCCTGCTAA AGCTAGCAGG 
CCAAAGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965980.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821434.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa31262
Clone ID Related Accession (Same CDS sequence)	XM_012965980.3 , XM_012965980.2
Accession Version	XM_012965980.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG TGTGCATCAA 
GATATGAAGG TATTTCCTCC TGGAAGCGAT GGGCATATTA AAATGCACTC AGGTGGAGAC 
TATCCTCTGG CTGCTGACAA CAAACACTTC CTTGAAATGA AAAATGAGTG TCCATCAAAG 
GGTCCATCTA GCTGCAGTTC TTTTCATAGC AGTGATGGAG AAGCAACTGA TCATGACCCT 
GACTTGCTCG ACTGCAGTGG ATCAAGACCT TTACTAATGG AATCAGATGA TGATGAATGT 
TCTAGGAAAA CTAAGGATTT AGAAGTTCCA AGTCCTCCTA TAGATGAAAT TAGCACCCAG 
AATTCTCAAC TTCCTTTTCA CAATGTAAAC TCTTGGCGGC CAGAGTTGAG CTTGGCTGAA 
GGTTTTGATG TATTCTCCAC TGCTCCATTC AAGGGTCCTA AATTAACACA GGATGAATCA 
GAAATATTTA CCAATGCTCC CTTTGTAGCC AAGACTGCAT TCCCTTCATT CCAGTCACAG 
GAACCTGATG TGTTCCTCAG GGCCCCTTTT TCCAGGAAAC TCAGTACCGA AGAAGCATCT 
CAAAATATTT CATCCCAATC ATTTTCGTCT CTACTTGGGC TTGATGTGGT AGGTAATTCT 
ACATTCCGAA ATTCTGAAAT GGCAGTTGGA TCCTCTTCAA TGAAGACCTC GTACACTACA 
GGCAATCTCT ATTCGCATTC CATCAGACCC TTAGAATCAG CTGACTCCTC TATTTTTACT 
AATACCTCAA TGGAACCAGG CATTATAGAG AATCACATGC TTCCAGGGCA CGAGGCTGCT 
CAAGAGACAG TATTTGGCAC GGTGGTCACA AAGCCTTTTC GCCCACAGTC ATTGTCAAAG 
TATTCCAGGC ACTATAGCCC TCAAGATGGG CGGAATGTGG ATGCTCAACC AATTGCTGCT 
TTCAAAGTCG TCTCCAAGAG CGCAAACACC TCTGTAGCTG GCTCTGTTCC TATATCCTCT 
GTGACTTACA GGACCTCAGA AATCACTAAT GTAGACCCAT TTGGCTCTGC TCCTTTTCCT 
GCTAAAGCTA GCAGGCCAAA GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821434.1
CDS	609..4055
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965980.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965980.3

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG TGTGCATCAA 
GATATGAAGG TATTTCCTCC TGGAAGCGAT GGGCATATTA AAATGCACTC AGGTGGAGAC 
TATCCTCTGG CTGCTGACAA CAAACACTTC CTTGAAATGA AAAATGAGTG TCCATCAAAG 
GGTCCATCTA GCTGCAGTTC TTTTCATAGC AGTGATGGAG AAGCAACTGA TCATGACCCT 
GACTTGCTCG ACTGCAGTGG ATCAAGACCT TTACTAATGG AATCAGATGA TGATGAATGT 
TCTAGGAAAA CTAAGGATTT AGAAGTTCCA AGTCCTCCTA TAGATGAAAT TAGCACCCAG 
AATTCTCAAC TTCCTTTTCA CAATGTAAAC TCTTGGCGGC CAGAGTTGAG CTTGGCTGAA 
GGTTTTGATG TATTCTCCAC TGCTCCATTC AAGGGTCCTA AATTAACACA GGATGAATCA 
GAAATATTTA CCAATGCTCC CTTTGTAGCC AAGACTGCAT TCCCTTCATT CCAGTCACAG 
GAACCTGATG TGTTCCTCAG GGCCCCTTTT TCCAGGAAAC TCAGTACCGA AGAAGCATCT 
CAAAATATTT CATCCCAATC ATTTTCGTCT CTACTTGGGC TTGATGTGGT AGGTAATTCT 
ACATTCCGAA ATTCTGAAAT GGCAGTTGGA TCCTCTTCAA TGAAGACCTC GTACACTACA 
GGCAATCTCT ATTCGCATTC CATCAGACCC TTAGAATCAG CTGACTCCTC TATTTTTACT 
AATACCTCAA TGGAACCAGG CATTATAGAG AATCACATGC TTCCAGGGCA CGAGGCTGCT 
CAAGAGACAG TATTTGGCAC GGTGGTCACA AAGCCTTTTC GCCCACAGTC ATTGTCAAAG 
TATTCCAGGC ACTATAGCCC TCAAGATGGG CGGAATGTGG ATGCTCAACC AATTGCTGCT 
TTCAAAGTCG TCTCCAAGAG CGCAAACACC TCTGTAGCTG GCTCTGTTCC TATATCCTCT 
GTGACTTACA GGACCTCAGA AATCACTAAT GTAGACCCAT TTGGCTCTGC TCCTTTTCCT 
GCTAAAGCTA GCAGGCCAAA GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965981.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821435.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X7, mRNA.

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CloneID	OXa31263
Clone ID Related Accession (Same CDS sequence)	XM_012965981.3 , XM_012965981.2
Accession Version	XM_012965981.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965981.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGAC GATACTAACC TGCTAACGGG CTTTGATATC 
CCGGACGATA CAGAAAAAAG GGCTGATGAT GAATTTGATC CTATTCCAGT TCTGGTATCC 
AAAAATTCCC AAGGTGTGCA TCAAGATATG AAGGTATTTC CTCCTGGAAG CGATGGGCAT 
ATTAAAATGC ACTCAGGTGG AGACTATCCT CTGGCTGCTG ACAACAAACA CTTCCTTGAA 
ATGAAAAATG AGTGTCCATC AAAGGGTCCA TCTAGCTGCA GTTCTTTTCA TAGCAGTGAT 
GGAGAAGCAA CTGATCATGA CCCTGACTTG CTCGACTGCA GTGGATCAAG ACCTTTACTA 
ATGGAATCAG ATGATGATGA ATGTTCTAGG AAAACTAAGG ATTTAGAAGT TCCAAGTCCT 
CCTATAGATG AAATTAGCAC CCAGAATTCT CAACTTCCTT TTCACAATGT AAACTCTTGG 
CGGCCAGAGT TGAGCTTGGC TGAAGGTTTT GATGTATTCT CCACTGCTCC ATTCAAGGGT 
CCTAAATTAA CACAGGATGA ATCAGAAATA TTTACCAATG CTCCCTTTGT AGCCAAGACT 
GCATTCCCTT CATTCCAGTC ACAGGAACCT GATGTGTTCC TCAGGGCCCC TTTTTCCAGG 
AAACTCAGTA CCGAAGAAGC ATCTCAAAAT ATTTCATCCC AATCATTTTC GTCTCTACTT 
GGGCTTGATG TGGTAGGTAA TTCTACATTC CGAAATTCTG AAATGGCAGT TGGATCCTCT 
TCAATGAAGA CCTCGTACAC TACAGGCAAT CTCTATTCGC ATTCCATCAG ACCCTTAGAA 
TCAGCTGACT CCTCTATTTT TACTAATACC TCAATGGAAC CAGGCATTAT AGAGAATCAC 
ATGCTTCCAG GGCACGAGGC TGCTCAAGAG ACAGTATTTG GCACGGTGGT CACAAAGCCT 
TTTCGCCCAC AGTCATTGTC AAAGTATTCC AGGCACTATA GCCCTCAAGA TGGGCGGAAT 
GTGGATGCTC AACCAATTGC TGCTTTCAAA GTCGTCTCCA AGAGCGCAAA CACCTCTGTA 
GCTGGCTCTG TTCCTATATC CTCTGTGACT TACAGGACCT CAGAAATCAC TAATGTAGAC 
CCATTTGGCT CTGCTCCTTT TCCTGCTAAA GCTAGCAGGC CAAAGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821435.1
CDS	608..3958
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965981.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965981.3

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGAC GATACTAACC TGCTAACGGG CTTTGATATC 
CCGGACGATA CAGAAAAAAG GGCTGATGAT GAATTTGATC CTATTCCAGT TCTGGTATCC 
AAAAATTCCC AAGGTGTGCA TCAAGATATG AAGGTATTTC CTCCTGGAAG CGATGGGCAT 
ATTAAAATGC ACTCAGGTGG AGACTATCCT CTGGCTGCTG ACAACAAACA CTTCCTTGAA 
ATGAAAAATG AGTGTCCATC AAAGGGTCCA TCTAGCTGCA GTTCTTTTCA TAGCAGTGAT 
GGAGAAGCAA CTGATCATGA CCCTGACTTG CTCGACTGCA GTGGATCAAG ACCTTTACTA 
ATGGAATCAG ATGATGATGA ATGTTCTAGG AAAACTAAGG ATTTAGAAGT TCCAAGTCCT 
CCTATAGATG AAATTAGCAC CCAGAATTCT CAACTTCCTT TTCACAATGT AAACTCTTGG 
CGGCCAGAGT TGAGCTTGGC TGAAGGTTTT GATGTATTCT CCACTGCTCC ATTCAAGGGT 
CCTAAATTAA CACAGGATGA ATCAGAAATA TTTACCAATG CTCCCTTTGT AGCCAAGACT 
GCATTCCCTT CATTCCAGTC ACAGGAACCT GATGTGTTCC TCAGGGCCCC TTTTTCCAGG 
AAACTCAGTA CCGAAGAAGC ATCTCAAAAT ATTTCATCCC AATCATTTTC GTCTCTACTT 
GGGCTTGATG TGGTAGGTAA TTCTACATTC CGAAATTCTG AAATGGCAGT TGGATCCTCT 
TCAATGAAGA CCTCGTACAC TACAGGCAAT CTCTATTCGC ATTCCATCAG ACCCTTAGAA 
TCAGCTGACT CCTCTATTTT TACTAATACC TCAATGGAAC CAGGCATTAT AGAGAATCAC 
ATGCTTCCAG GGCACGAGGC TGCTCAAGAG ACAGTATTTG GCACGGTGGT CACAAAGCCT 
TTTCGCCCAC AGTCATTGTC AAAGTATTCC AGGCACTATA GCCCTCAAGA TGGGCGGAAT 
GTGGATGCTC AACCAATTGC TGCTTTCAAA GTCGTCTCCA AGAGCGCAAA CACCTCTGTA 
GCTGGCTCTG TTCCTATATC CTCTGTGACT TACAGGACCT CAGAAATCAC TAATGTAGAC 
CCATTTGGCT CTGCTCCTTT TCCTGCTAAA GCTAGCAGGC CAAAGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965982.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821436.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X8, mRNA.

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CloneID	OXa31264
Clone ID Related Accession (Same CDS sequence)	XM_012965982.3 , XM_012965982.2
Accession Version	XM_012965982.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965982.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGAA AAATTGATTG AGGGTCTCAA ATCTCCAGAG 
CCCCAGCTTC TCCTCCCTCA TCTTGTGCAG CTGGCAGATC CCTTTGGGAG CACAGCAGAG 
GATGGAAATG GTGTGCATCA AGATATGAAG GTATTTCCTC CTGGAAGCGA TGGGCATATT 
AAAATGCACT CAGGTGGAGA CTATCCTCTG GCTGCTGACA ACAAACACTT CCTTGAAATG 
AAAAATGAGT GTCCATCAAA GGGTCCATCT AGCTGCAGTT CTTTTCATAG CAGTGATGGA 
GAAGCAACTG ATCATGACCC TGACTTGCTC GACTGCAGTG GATCAAGACC TTTACTAATG 
GAATCAGATG ATGATGAATG TTCTAGGAAA ACTAAGGATT TAGAAGTTCC AAGTCCTCCT 
ATAGATGAAA TTAGCACCCA GAATTCTCAA CTTCCTTTTC ACAATGTAAA CTCTTGGCGG 
CCAGAGTTGA GCTTGGCTGA AGGTTTTGAT GTATTCTCCA CTGCTCCATT CAAGGGTCCT 
AAATTAACAC AGGATGAATC AGAAATATTT ACCAATGCTC CCTTTGTAGC CAAGACTGCA 
TTCCCTTCAT TCCAGTCACA GGAACCTGAT GTGTTCCTCA GGGCCCCTTT TTCCAGGAAA 
CTCAGTACCG AAGAAGCATC TCAAAATATT TCATCCCAAT CATTTTCGTC TCTACTTGGG 
CTTGATGTGG TAGGTAATTC TACATTCCGA AATTCTGAAA TGGCAGTTGG ATCCTCTTCA 
ATGAAGACCT CGTACACTAC AGGCAATCTC TATTCGCATT CCATCAGACC CTTAGAATCA 
GCTGACTCCT CTATTTTTAC TAATACCTCA ATGGAACCAG GCATTATAGA GAATCACATG 
CTTCCAGGGC ACGAGGCTGC TCAAGAGACA GTATTTGGCA CGGTGGTCAC AAAGCCTTTT 
CGCCCACAGT CATTGTCAAA GTATTCCAGG CACTATAGCC CTCAAGATGG GCGGAATGTG 
GATGCTCAAC CAATTGCTGC TTTCAAAGTC GTCTCCAAGA GCGCAAACAC CTCTGTAGCT 
GGCTCTGTTC CTATATCCTC TGTGACTTAC AGGACCTCAG AAATCACTAA TGTAGACCCA 
TTTGGCTCTG CTCCTTTTCC TGCTAAAGCT AGCAGGCCAA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821436.1
CDS	608..3955
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965982.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965982.3

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGAA AAATTGATTG AGGGTCTCAA ATCTCCAGAG 
CCCCAGCTTC TCCTCCCTCA TCTTGTGCAG CTGGCAGATC CCTTTGGGAG CACAGCAGAG 
GATGGAAATG GTGTGCATCA AGATATGAAG GTATTTCCTC CTGGAAGCGA TGGGCATATT 
AAAATGCACT CAGGTGGAGA CTATCCTCTG GCTGCTGACA ACAAACACTT CCTTGAAATG 
AAAAATGAGT GTCCATCAAA GGGTCCATCT AGCTGCAGTT CTTTTCATAG CAGTGATGGA 
GAAGCAACTG ATCATGACCC TGACTTGCTC GACTGCAGTG GATCAAGACC TTTACTAATG 
GAATCAGATG ATGATGAATG TTCTAGGAAA ACTAAGGATT TAGAAGTTCC AAGTCCTCCT 
ATAGATGAAA TTAGCACCCA GAATTCTCAA CTTCCTTTTC ACAATGTAAA CTCTTGGCGG 
CCAGAGTTGA GCTTGGCTGA AGGTTTTGAT GTATTCTCCA CTGCTCCATT CAAGGGTCCT 
AAATTAACAC AGGATGAATC AGAAATATTT ACCAATGCTC CCTTTGTAGC CAAGACTGCA 
TTCCCTTCAT TCCAGTCACA GGAACCTGAT GTGTTCCTCA GGGCCCCTTT TTCCAGGAAA 
CTCAGTACCG AAGAAGCATC TCAAAATATT TCATCCCAAT CATTTTCGTC TCTACTTGGG 
CTTGATGTGG TAGGTAATTC TACATTCCGA AATTCTGAAA TGGCAGTTGG ATCCTCTTCA 
ATGAAGACCT CGTACACTAC AGGCAATCTC TATTCGCATT CCATCAGACC CTTAGAATCA 
GCTGACTCCT CTATTTTTAC TAATACCTCA ATGGAACCAG GCATTATAGA GAATCACATG 
CTTCCAGGGC ACGAGGCTGC TCAAGAGACA GTATTTGGCA CGGTGGTCAC AAAGCCTTTT 
CGCCCACAGT CATTGTCAAA GTATTCCAGG CACTATAGCC CTCAAGATGG GCGGAATGTG 
GATGCTCAAC CAATTGCTGC TTTCAAAGTC GTCTCCAAGA GCGCAAACAC CTCTGTAGCT 
GGCTCTGTTC CTATATCCTC TGTGACTTAC AGGACCTCAG AAATCACTAA TGTAGACCCA 
TTTGGCTCTG CTCCTTTTCC TGCTAAAGCT AGCAGGCCAA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( NM_001114046.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001107518.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), mRNA.

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CloneID	OXa25095
Clone ID Related Accession (Same CDS sequence)	NM_001114046.1
Accession Version	NM_001114046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC157684.1. ##Evidence-Data-START## Transcript exon combination :: BC157684.1, BC171117.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028291, SAMD00028293 [ECO:0000348] ##Evidence-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGGT GTGCATCAAG ATATGAAGGT ATTTCCTCCT 
GGAAGCGATG GGCATATTAA AATGCACTCA GGTGGAGACT ATCCTCTGGC TGCTGACAAC 
AAACACTTCC TTGAAATGAA AAATGAGTGT CCATCAAAGG GTCCATCTAG CTGCAGTTCT 
TTTCATAGCA GTGATGGAGA AGCAACTGAT CATGACCCTG ACTTGCTCGA CTGCAGTGGA 
TCAAGACCTT TACTAATGGA ATCAGATGAT GATGAATGTT CTAGGAAAAC TAAGGATTTA 
GAAGTTCCAA GTCCTCCTAT AGATGAAATT AGCACCCAGA ATTCTCAACT TCCTTTTCAC 
AATGTAAACT CTTGGCGGCC AGAGTTGAGC TTGGCTGAAG GTTTTGATGT ATTCTCCACT 
GCTCCATTCA AGGGTCCTAA ATTAACACAG GATGAATCAG AAATATTTAC CAATGCTCCC 
TTTGTAGCCA AGACTGCATT CCCTTCATTC CAGTCACAGG AACCTGATGT GTTCCTCAGG 
GCCCCTTTTT CCAGGAAACT CAGTACCGAA GAAGCATCTC AAAATATTTC ATCCCAATCA 
TTTTCGTCTC TACTTGGGCT TGATGTGGTA GGTAATTCTA CATTCCGAAA TTCTGAAATG 
GCAGTTGGAT CCTCTTCAAT GAAGACCTCG TACACTACAG GCAATCTCTA TTCGCATTCC 
ATCAGACCCT TAGAATCAGC TGACTCCTCT ATTTTTACTA ATACCTCAAT GGAACCAGGC 
ATTATAGAGA ATCACATGCT TCCAGGGCAC GAGGCTGCTC AAGAGACAGT ATTTGGCACG 
GTGGTCACAA AGCCTTTTCG CCCACAGTCA TTGTCAAAGT ATTCCAGGCA CTATAGCCCT 
CAAGATGGGC GGAATGTGGA TGCTCAACCA ATTGCTGCTT TCAAAGTCGT CTCCAAGAGC 
GCAAACACCT CTGTAGCTGG CTCTGTTCCT ATATCCTCTG TGACTTACAG GACCTCAGAA 
ATCACTAATG TAGACCCATT TGGCTCTGCT CCTTTTCCTG CTAAAGCTAG CAGGCCAAAG 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107518.1
CDS	338..3583
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	NM_001114046.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114046.1

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGGT GTGCATCAAG ATATGAAGGT ATTTCCTCCT 
GGAAGCGATG GGCATATTAA AATGCACTCA GGTGGAGACT ATCCTCTGGC TGCTGACAAC 
AAACACTTCC TTGAAATGAA AAATGAGTGT CCATCAAAGG GTCCATCTAG CTGCAGTTCT 
TTTCATAGCA GTGATGGAGA AGCAACTGAT CATGACCCTG ACTTGCTCGA CTGCAGTGGA 
TCAAGACCTT TACTAATGGA ATCAGATGAT GATGAATGTT CTAGGAAAAC TAAGGATTTA 
GAAGTTCCAA GTCCTCCTAT AGATGAAATT AGCACCCAGA ATTCTCAACT TCCTTTTCAC 
AATGTAAACT CTTGGCGGCC AGAGTTGAGC TTGGCTGAAG GTTTTGATGT ATTCTCCACT 
GCTCCATTCA AGGGTCCTAA ATTAACACAG GATGAATCAG AAATATTTAC CAATGCTCCC 
TTTGTAGCCA AGACTGCATT CCCTTCATTC CAGTCACAGG AACCTGATGT GTTCCTCAGG 
GCCCCTTTTT CCAGGAAACT CAGTACCGAA GAAGCATCTC AAAATATTTC ATCCCAATCA 
TTTTCGTCTC TACTTGGGCT TGATGTGGTA GGTAATTCTA CATTCCGAAA TTCTGAAATG 
GCAGTTGGAT CCTCTTCAAT GAAGACCTCG TACACTACAG GCAATCTCTA TTCGCATTCC 
ATCAGACCCT TAGAATCAGC TGACTCCTCT ATTTTTACTA ATACCTCAAT GGAACCAGGC 
ATTATAGAGA ATCACATGCT TCCAGGGCAC GAGGCTGCTC AAGAGACAGT ATTTGGCACG 
GTGGTCACAA AGCCTTTTCG CCCACAGTCA TTGTCAAAGT ATTCCAGGCA CTATAGCCCT 
CAAGATGGGC GGAATGTGGA TGCTCAACCA ATTGCTGCTT TCAAAGTCGT CTCCAAGAGC 
GCAAACACCT CTGTAGCTGG CTCTGTTCCT ATATCCTCTG TGACTTACAG GACCTCAGAA 
ATCACTAATG TAGACCCATT TGGCTCTGCT CCTTTTCCTG CTAAAGCTAG CAGGCCAAAG 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965972.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821426.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31255
Clone ID Related Accession (Same CDS sequence)	XM_012965972.2 , XM_012965972.3
Accession Version	XM_012965972.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG GAAATCAGAG 
CTGGAGGTAG AAAGCCTCAT TCCTGGGCTG GAGCCCCCAA TGCCCCAGCG ACTTTCATCC 
CAGGCAGACT CTATTGCTTC AAACCGAACA GACTCCCTCA CTGGGGAAGA TTCTCTCCTT 
GACTACTCTC TTCTCTCTAA CCCCGCGGCT GATCTTCTTG ACGAGTTTGC TCCTGTAACA 
CATTCGGCTC TGGCTTCCAC AGACGATACT AACCTGCTAA CGGGCTTTGA TATCCCGGAC 
GATACAGAAA AAAGGGCTGA TGATGAATTT GATCCTATTC CAGTTCTGGT ATCCAAAAAT 
TCCCAAGGTG TGCATCAAGA TATGAAGGTA TTTCCTCCTG GAAGCGATGG GCATATTAAA 
ATGCACTCAG GTGGAGACTA TCCTCTGGCT GCTGACAACA AACACTTCCT TGAAATGAAA 
AATGAGTGTC CATCAAAGGG TCCATCTAGC TGCAGTTCTT TTCATAGCAG TGATGGAGAA 
GCAACTGATC ATGACCCTGA CTTGCTCGAC TGCAGTGGAT CAAGACCTTT ACTAATGGAA 
TCAGATGATG ATGAATGTTC TAGGAAAACT AAGGATTTAG AAGTTCCAAG TCCTCCTATA 
GATGAAATTA GCACCCAGAA TTCTCAACTT CCTTTTCACA ATGTAAACTC TTGGCGGCCA 
GAGTTGAGCT TGGCTGAAGG TTTTGATGTA TTCTCCACTG CTCCATTCAA GGGTCCTAAA 
TTAACACAGG ATGAATCAGA AATATTTACC AATGCTCCCT TTGTAGCCAA GACTGCATTC 
CCTTCATTCC AGTCACAGGA ACCTGATGTG TTCCTCAGGG CCCCTTTTTC CAGGAAACTC 
AGTACCGAAG AAGCATCTCA AAATATTTCA TCCCAATCAT TTTCGTCTCT ACTTGGGCTT 
GATGTGGTAG GTAATTCTAC ATTCCGAAAT TCTGAAATGG CAGTTGGATC CTCTTCAATG 
AAGACCTCGT ACACTACAGG CAATCTCTAT TCGCATTCCA TCAGACCCTT AGAATCAGCT 
GACTCCTCTA TTTTTACTAA TACCTCAATG GAACCAGGCA TTATAGAGAA TCACATGCTT 
CCAGGGCACG AGGCTGCTCA AGAGACAGTA TTTGGCACGG TGGTCACAAA GCCTTTTCGC 
CCACAGTCAT TGTCAAAGTA TTCCAGGCAC TATAGCCCTC AAGATGGGCG GAATGTGGAT 
GCTCAACCAA TTGCTGCTTT CAAAGTCGTC TCCAAGAGCG CAAACACCTC TGTAGCTGGC 
TCTGTTCCTA TATCCTCTGT GACTTACAGG ACCTCAGAAA TCACTAATGT AGACCCATTT 
GGCTCTGCTC CTTTTCCTGC TAAAGCTAGC AGGCCAAAGC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821426.1
CDS	1041..4805
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGKSELEVESLIPGLEPPMPQRLSSQADSIASNRTDSLTGEDSLLDYSLLSNPAADLLDEFAPVTHSALASTDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965972.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965972.2

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG GAAATCAGAG 
CTGGAGGTAG AAAGCCTCAT TCCTGGGCTG GAGCCCCCAA TGCCCCAGCG ACTTTCATCC 
CAGGCAGACT CTATTGCTTC AAACCGAACA GACTCCCTCA CTGGGGAAGA TTCTCTCCTT 
GACTACTCTC TTCTCTCTAA CCCCGCGGCT GATCTTCTTG ACGAGTTTGC TCCTGTAACA 
CATTCGGCTC TGGCTTCCAC AGACGATACT AACCTGCTAA CGGGCTTTGA TATCCCGGAC 
GATACAGAAA AAAGGGCTGA TGATGAATTT GATCCTATTC CAGTTCTGGT ATCCAAAAAT 
TCCCAAGGTG TGCATCAAGA TATGAAGGTA TTTCCTCCTG GAAGCGATGG GCATATTAAA 
ATGCACTCAG GTGGAGACTA TCCTCTGGCT GCTGACAACA AACACTTCCT TGAAATGAAA 
AATGAGTGTC CATCAAAGGG TCCATCTAGC TGCAGTTCTT TTCATAGCAG TGATGGAGAA 
GCAACTGATC ATGACCCTGA CTTGCTCGAC TGCAGTGGAT CAAGACCTTT ACTAATGGAA 
TCAGATGATG ATGAATGTTC TAGGAAAACT AAGGATTTAG AAGTTCCAAG TCCTCCTATA 
GATGAAATTA GCACCCAGAA TTCTCAACTT CCTTTTCACA ATGTAAACTC TTGGCGGCCA 
GAGTTGAGCT TGGCTGAAGG TTTTGATGTA TTCTCCACTG CTCCATTCAA GGGTCCTAAA 
TTAACACAGG ATGAATCAGA AATATTTACC AATGCTCCCT TTGTAGCCAA GACTGCATTC 
CCTTCATTCC AGTCACAGGA ACCTGATGTG TTCCTCAGGG CCCCTTTTTC CAGGAAACTC 
AGTACCGAAG AAGCATCTCA AAATATTTCA TCCCAATCAT TTTCGTCTCT ACTTGGGCTT 
GATGTGGTAG GTAATTCTAC ATTCCGAAAT TCTGAAATGG CAGTTGGATC CTCTTCAATG 
AAGACCTCGT ACACTACAGG CAATCTCTAT TCGCATTCCA TCAGACCCTT AGAATCAGCT 
GACTCCTCTA TTTTTACTAA TACCTCAATG GAACCAGGCA TTATAGAGAA TCACATGCTT 
CCAGGGCACG AGGCTGCTCA AGAGACAGTA TTTGGCACGG TGGTCACAAA GCCTTTTCGC 
CCACAGTCAT TGTCAAAGTA TTCCAGGCAC TATAGCCCTC AAGATGGGCG GAATGTGGAT 
GCTCAACCAA TTGCTGCTTT CAAAGTCGTC TCCAAGAGCG CAAACACCTC TGTAGCTGGC 
TCTGTTCCTA TATCCTCTGT GACTTACAGG ACCTCAGAAA TCACTAATGT AGACCCATTT 
GGCTCTGCTC CTTTTCCTGC TAAAGCTAGC AGGCCAAAGC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965973.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821427.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa31256
Clone ID Related Accession (Same CDS sequence)	XM_012965973.2 , XM_012965973.3
Accession Version	XM_012965973.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965973.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCCAGC TCCACTCAAG 
ACCCCACAGA AGCCCCAGCC ACCTACAGCA CCTCCATCTT CTGCACCTCC CACAGCAGCA 
GGGCAGAAAC TGGGATCCCT TACCCCTCCT TCATCCCCCA AGACCCAAAG AGGCGGCCAC 
AGAAGGATAC TTAGTGATGT TACGCATAGT GCAGTTTTCG GTGTCCCAGC CAGTAAATCT 
ACTCAGCTTC TACAGGCTGC TGCGGCTGAA GCAAACCTCA ACAAATCAAA GTCAGCTACA 
ACCACCCCTT CTGGCTCACC TCGGACCTCC CAGCAGAATG TTCACAATCC GCCAGAACCT 
TCTGGCTGGA ATCCTTTCGA CGATGACAAC TTTTCCAAAC TCACAGCTGA GGAACTTCTA 
AATAAGGATT TTGCCAAGCT TGGAGAAAGT AAGCCATCGG AGCAGCTGAG CCGCTCAGCA 
GAAGATCTCC TATCTGGGTT TCCAGCTCCA CCTGCTGTAT CTCAGGCTGA TTCATTTGGA 
ACTGCACTCT TTGCCAGTGG TTCAGCTGCA GATAAACCTG TAGATTCACA GGGGCCCAGT 
CCTGGCTCTC AGCTGCAAAA AACTTCTGAT TCTTTCTTGC CTCTCCCTCT GTCTGACACC 
CCAGAAAAAT TGATTGAGGG TCTCAAATCT CCAGAGCCCC AGCTTCTCCT CCCTCATCTT 
GTGCAGCTGG CAGATCCCTT TGGGAGCACA GCAGAGGATG GAAATGGGAA ATCAGAGCTG 
GAGGTAGAAA GCCTCATTCC TGGGCTGGAG CCCCCAATGC CCCAGCGACT TTCATCCCAG 
GCAGACTCTA TTGCTTCAAA CCGAACAGAC TCCCTCACTG GGGAAGATTC TCTCCTTGAC 
TACTCTCTTC TCTCTAACCC CGCGGCTGAT CTTCTTGACG AGTTTGCTCC TGTAACACAT 
TCGGCTCTGG CTTCCACAGA CGATACTAAC CTGCTAACGG GCTTTGATAT CCCGGACGAT 
ACAGAAAAAA GGGCTGATGA TGAATTTGAT CCTATTCCAG TTCTGGTATC CAAAAATTCC 
CAAGGTGTGC ATCAAGATAT GAAGGTATTT CCTCCTGGAA GCGATGGGCA TATTAAAATG 
CACTCAGGTG GAGACTATCC TCTGGCTGCT GACAACAAAC ACTTCCTTGA AATGAAAAAT 
GAGTGTCCAT CAAAGGGTCC ATCTAGCTGC AGTTCTTTTC ATAGCAGTGA TGGAGAAGCA 
ACTGATCATG ACCCTGACTT GCTCGACTGC AGTGGATCAA GACCTTTACT AATGGAATCA 
GATGATGATG AATGTTCTAG GAAAACTAAG GATTTAGAAG TTCCAAGTCC TCCTATAGAT 
GAAATTAGCA CCCAGAATTC TCAACTTCCT TTTCACAATG TAAACTCTTG GCGGCCAGAG 
TTGAGCTTGG CTGAAGGTTT TGATGTATTC TCCACTGCTC CATTCAAGGG TCCTAAATTA 
ACACAGGATG AATCAGAAAT ATTTACCAAT GCTCCCTTTG TAGCCAAGAC TGCATTCCCT 
TCATTCCAGT CACAGGAACC TGATGTGTTC CTCAGGGCCC CTTTTTCCAG GAAACTCAGT 
ACCGAAGAAG CATCTCAAAA TATTTCATCC CAATCATTTT CGTCTCTACT TGGGCTTGAT 
GTGGTAGGTA ATTCTACATT CCGAAATTCT GAAATGGCAG TTGGATCCTC TTCAATGAAG 
ACCTCGTACA CTACAGGCAA TCTCTATTCG CATTCCATCA GACCCTTAGA ATCAGCTGAC 
TCCTCTATTT TTACTAATAC CTCAATGGAA CCAGGCATTA TAGAGAATCA CATGCTTCCA 
GGGCACGAGG CTGCTCAAGA GACAGTATTT GGCACGGTGG TCACAAAGCC TTTTCGCCCA 
CAGTCATTGT CAAAGTATTC CAGGCACTAT AGCCCTCAAG ATGGGCGGAA TGTGGATGCT 
CAACCAATTG CTGCTTTCAA AGTCGTCTCC AAGAGCGCAA ACACCTCTGT AGCTGGCTCT 
GTTCCTATAT CCTCTGTGAC TTACAGGACC TCAGAAATCA CTAATGTAGA CCCATTTGGC 
TCTGCTCCTT TTCCTGCTAA AGCTAGCAGG CCAAAGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821427.1
CDS	1043..4804
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGKSELEVESLIPGLEPPMPQRLSSQADSIASNRTDSLTGEDSLLDYSLLSNPAADLLDEFAPVTHSALASTDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965973.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965973.2

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCCAGC TCCACTCAAG 
ACCCCACAGA AGCCCCAGCC ACCTACAGCA CCTCCATCTT CTGCACCTCC CACAGCAGCA 
GGGCAGAAAC TGGGATCCCT TACCCCTCCT TCATCCCCCA AGACCCAAAG AGGCGGCCAC 
AGAAGGATAC TTAGTGATGT TACGCATAGT GCAGTTTTCG GTGTCCCAGC CAGTAAATCT 
ACTCAGCTTC TACAGGCTGC TGCGGCTGAA GCAAACCTCA ACAAATCAAA GTCAGCTACA 
ACCACCCCTT CTGGCTCACC TCGGACCTCC CAGCAGAATG TTCACAATCC GCCAGAACCT 
TCTGGCTGGA ATCCTTTCGA CGATGACAAC TTTTCCAAAC TCACAGCTGA GGAACTTCTA 
AATAAGGATT TTGCCAAGCT TGGAGAAAGT AAGCCATCGG AGCAGCTGAG CCGCTCAGCA 
GAAGATCTCC TATCTGGGTT TCCAGCTCCA CCTGCTGTAT CTCAGGCTGA TTCATTTGGA 
ACTGCACTCT TTGCCAGTGG TTCAGCTGCA GATAAACCTG TAGATTCACA GGGGCCCAGT 
CCTGGCTCTC AGCTGCAAAA AACTTCTGAT TCTTTCTTGC CTCTCCCTCT GTCTGACACC 
CCAGAAAAAT TGATTGAGGG TCTCAAATCT CCAGAGCCCC AGCTTCTCCT CCCTCATCTT 
GTGCAGCTGG CAGATCCCTT TGGGAGCACA GCAGAGGATG GAAATGGGAA ATCAGAGCTG 
GAGGTAGAAA GCCTCATTCC TGGGCTGGAG CCCCCAATGC CCCAGCGACT TTCATCCCAG 
GCAGACTCTA TTGCTTCAAA CCGAACAGAC TCCCTCACTG GGGAAGATTC TCTCCTTGAC 
TACTCTCTTC TCTCTAACCC CGCGGCTGAT CTTCTTGACG AGTTTGCTCC TGTAACACAT 
TCGGCTCTGG CTTCCACAGA CGATACTAAC CTGCTAACGG GCTTTGATAT CCCGGACGAT 
ACAGAAAAAA GGGCTGATGA TGAATTTGAT CCTATTCCAG TTCTGGTATC CAAAAATTCC 
CAAGGTGTGC ATCAAGATAT GAAGGTATTT CCTCCTGGAA GCGATGGGCA TATTAAAATG 
CACTCAGGTG GAGACTATCC TCTGGCTGCT GACAACAAAC ACTTCCTTGA AATGAAAAAT 
GAGTGTCCAT CAAAGGGTCC ATCTAGCTGC AGTTCTTTTC ATAGCAGTGA TGGAGAAGCA 
ACTGATCATG ACCCTGACTT GCTCGACTGC AGTGGATCAA GACCTTTACT AATGGAATCA 
GATGATGATG AATGTTCTAG GAAAACTAAG GATTTAGAAG TTCCAAGTCC TCCTATAGAT 
GAAATTAGCA CCCAGAATTC TCAACTTCCT TTTCACAATG TAAACTCTTG GCGGCCAGAG 
TTGAGCTTGG CTGAAGGTTT TGATGTATTC TCCACTGCTC CATTCAAGGG TCCTAAATTA 
ACACAGGATG AATCAGAAAT ATTTACCAAT GCTCCCTTTG TAGCCAAGAC TGCATTCCCT 
TCATTCCAGT CACAGGAACC TGATGTGTTC CTCAGGGCCC CTTTTTCCAG GAAACTCAGT 
ACCGAAGAAG CATCTCAAAA TATTTCATCC CAATCATTTT CGTCTCTACT TGGGCTTGAT 
GTGGTAGGTA ATTCTACATT CCGAAATTCT GAAATGGCAG TTGGATCCTC TTCAATGAAG 
ACCTCGTACA CTACAGGCAA TCTCTATTCG CATTCCATCA GACCCTTAGA ATCAGCTGAC 
TCCTCTATTT TTACTAATAC CTCAATGGAA CCAGGCATTA TAGAGAATCA CATGCTTCCA 
GGGCACGAGG CTGCTCAAGA GACAGTATTT GGCACGGTGG TCACAAAGCC TTTTCGCCCA 
CAGTCATTGT CAAAGTATTC CAGGCACTAT AGCCCTCAAG ATGGGCGGAA TGTGGATGCT 
CAACCAATTG CTGCTTTCAA AGTCGTCTCC AAGAGCGCAA ACACCTCTGT AGCTGGCTCT 
GTTCCTATAT CCTCTGTGAC TTACAGGACC TCAGAAATCA CTAATGTAGA CCCATTTGGC 
TCTGCTCCTT TTCCTGCTAA AGCTAGCAGG CCAAAGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965974.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821428.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X3, mRNA.

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CloneID	OXa31257
Clone ID Related Accession (Same CDS sequence)	XM_012965974.2 , XM_012965974.3
Accession Version	XM_012965974.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGAA AAATTGATTG AGGGTCTCAA ATCTCCAGAG 
CCCCAGCTTC TCCTCCCTCA TCTTGTGCAG CTGGCAGATC CCTTTGGGAG CACAGCAGAG 
GATGGAAATG GGAAATCAGA GCTGGAGGTA GAAAGCCTCA TTCCTGGGCT GGAGCCCCCA 
ATGCCCCAGC GACTTTCATC CCAGGCAGAC TCTATTGCTT CAAACCGAAC AGACTCCCTC 
ACTGGGGAAG ATTCTCTCCT TGACTACTCT CTTCTCTCTA ACCCCGCGGC TGATCTTCTT 
GACGAGTTTG CTCCTGTAAC ACATTCGGCT CTGGCTTCCA CAGACGATAC TAACCTGCTA 
ACGGGCTTTG ATATCCCGGA CGATACAGAA AAAAGGGCTG ATGATGAATT TGATCCTATT 
CCAGTTCTGG TATCCAAAAA TTCCCAAGGT GTGCATCAAG ATATGAAGGT ATTTCCTCCT 
GGAAGCGATG GGCATATTAA AATGCACTCA GGTGGAGACT ATCCTCTGGC TGCTGACAAC 
AAACACTTCC TTGAAATGAA AAATGAGTGT CCATCAAAGG GTCCATCTAG CTGCAGTTCT 
TTTCATAGCA GTGATGGAGA AGCAACTGAT CATGACCCTG ACTTGCTCGA CTGCAGTGGA 
TCAAGACCTT TACTAATGGA ATCAGATGAT GATGAATGTT CTAGGAAAAC TAAGGATTTA 
GAAGTTCCAA GTCCTCCTAT AGATGAAATT AGCACCCAGA ATTCTCAACT TCCTTTTCAC 
AATGTAAACT CTTGGCGGCC AGAGTTGAGC TTGGCTGAAG GTTTTGATGT ATTCTCCACT 
GCTCCATTCA AGGGTCCTAA ATTAACACAG GATGAATCAG AAATATTTAC CAATGCTCCC 
TTTGTAGCCA AGACTGCATT CCCTTCATTC CAGTCACAGG AACCTGATGT GTTCCTCAGG 
GCCCCTTTTT CCAGGAAACT CAGTACCGAA GAAGCATCTC AAAATATTTC ATCCCAATCA 
TTTTCGTCTC TACTTGGGCT TGATGTGGTA GGTAATTCTA CATTCCGAAA TTCTGAAATG 
GCAGTTGGAT CCTCTTCAAT GAAGACCTCG TACACTACAG GCAATCTCTA TTCGCATTCC 
ATCAGACCCT TAGAATCAGC TGACTCCTCT ATTTTTACTA ATACCTCAAT GGAACCAGGC 
ATTATAGAGA ATCACATGCT TCCAGGGCAC GAGGCTGCTC AAGAGACAGT ATTTGGCACG 
GTGGTCACAA AGCCTTTTCG CCCACAGTCA TTGTCAAAGT ATTCCAGGCA CTATAGCCCT 
CAAGATGGGC GGAATGTGGA TGCTCAACCA ATTGCTGCTT TCAAAGTCGT CTCCAAGAGC 
GCAAACACCT CTGTAGCTGG CTCTGTTCCT ATATCCTCTG TGACTTACAG GACCTCAGAA 
ATCACTAATG TAGACCCATT TGGCTCTGCT CCTTTTCCTG CTAAAGCTAG CAGGCCAAAG 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821428.1
CDS	1040..4705
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGKSELEVESLIPGLEPPMPQRLSSQADSIASNRTDSLTGEDSLLDYSLLSNPAADLLDEFAPVTHSALASTDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965974.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965974.2

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGAA AAATTGATTG AGGGTCTCAA ATCTCCAGAG 
CCCCAGCTTC TCCTCCCTCA TCTTGTGCAG CTGGCAGATC CCTTTGGGAG CACAGCAGAG 
GATGGAAATG GGAAATCAGA GCTGGAGGTA GAAAGCCTCA TTCCTGGGCT GGAGCCCCCA 
ATGCCCCAGC GACTTTCATC CCAGGCAGAC TCTATTGCTT CAAACCGAAC AGACTCCCTC 
ACTGGGGAAG ATTCTCTCCT TGACTACTCT CTTCTCTCTA ACCCCGCGGC TGATCTTCTT 
GACGAGTTTG CTCCTGTAAC ACATTCGGCT CTGGCTTCCA CAGACGATAC TAACCTGCTA 
ACGGGCTTTG ATATCCCGGA CGATACAGAA AAAAGGGCTG ATGATGAATT TGATCCTATT 
CCAGTTCTGG TATCCAAAAA TTCCCAAGGT GTGCATCAAG ATATGAAGGT ATTTCCTCCT 
GGAAGCGATG GGCATATTAA AATGCACTCA GGTGGAGACT ATCCTCTGGC TGCTGACAAC 
AAACACTTCC TTGAAATGAA AAATGAGTGT CCATCAAAGG GTCCATCTAG CTGCAGTTCT 
TTTCATAGCA GTGATGGAGA AGCAACTGAT CATGACCCTG ACTTGCTCGA CTGCAGTGGA 
TCAAGACCTT TACTAATGGA ATCAGATGAT GATGAATGTT CTAGGAAAAC TAAGGATTTA 
GAAGTTCCAA GTCCTCCTAT AGATGAAATT AGCACCCAGA ATTCTCAACT TCCTTTTCAC 
AATGTAAACT CTTGGCGGCC AGAGTTGAGC TTGGCTGAAG GTTTTGATGT ATTCTCCACT 
GCTCCATTCA AGGGTCCTAA ATTAACACAG GATGAATCAG AAATATTTAC CAATGCTCCC 
TTTGTAGCCA AGACTGCATT CCCTTCATTC CAGTCACAGG AACCTGATGT GTTCCTCAGG 
GCCCCTTTTT CCAGGAAACT CAGTACCGAA GAAGCATCTC AAAATATTTC ATCCCAATCA 
TTTTCGTCTC TACTTGGGCT TGATGTGGTA GGTAATTCTA CATTCCGAAA TTCTGAAATG 
GCAGTTGGAT CCTCTTCAAT GAAGACCTCG TACACTACAG GCAATCTCTA TTCGCATTCC 
ATCAGACCCT TAGAATCAGC TGACTCCTCT ATTTTTACTA ATACCTCAAT GGAACCAGGC 
ATTATAGAGA ATCACATGCT TCCAGGGCAC GAGGCTGCTC AAGAGACAGT ATTTGGCACG 
GTGGTCACAA AGCCTTTTCG CCCACAGTCA TTGTCAAAGT ATTCCAGGCA CTATAGCCCT 
CAAGATGGGC GGAATGTGGA TGCTCAACCA ATTGCTGCTT TCAAAGTCGT CTCCAAGAGC 
GCAAACACCT CTGTAGCTGG CTCTGTTCCT ATATCCTCTG TGACTTACAG GACCTCAGAA 
ATCACTAATG TAGACCCATT TGGCTCTGCT CCTTTTCCTG CTAAAGCTAG CAGGCCAAAG 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965975.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821429.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X4, mRNA.

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CloneID	OXa31258
Clone ID Related Accession (Same CDS sequence)	XM_012965975.2
Accession Version	XM_012965975.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG GAAATCAGAG 
CTGGAGGTAG AAAGCCTCAT TCCTGGGCTG GAGCCCCCAA TGCCCCAGCG ACTTTCATCC 
CAGGCAGACT CTATTGCTTC AAACCGAACA GACGATACTA ACCTGCTAAC GGGCTTTGAT 
ATCCCGGACG ATACAGAAAA AAGGGCTGAT GATGAATTTG ATCCTATTCC AGTTCTGGTA 
TCCAAAAATT CCCAAGGTGT GCATCAAGAT ATGAAGGTAT TTCCTCCTGG AAGCGATGGG 
CATATTAAAA TGCACTCAGG TGGAGACTAT CCTCTGGCTG CTGACAACAA ACACTTCCTT 
GAAATGAAAA ATGAGTGTCC ATCAAAGGGT CCATCTAGCT GCAGTTCTTT TCATAGCAGT 
GATGGAGAAG CAACTGATCA TGACCCTGAC TTGCTCGACT GCAGTGGATC AAGACCTTTA 
CTAATGGAAT CAGATGATGA TGAATGTTCT AGGAAAACTA AGGATTTAGA AGTTCCAAGT 
CCTCCTATAG ATGAAATTAG CACCCAGAAT TCTCAACTTC CTTTTCACAA TGTAAACTCT 
TGGCGGCCAG AGTTGAGCTT GGCTGAAGGT TTTGATGTAT TCTCCACTGC TCCATTCAAG 
GGTCCTAAAT TAACACAGGA TGAATCAGAA ATATTTACCA ATGCTCCCTT TGTAGCCAAG 
ACTGCATTCC CTTCATTCCA GTCACAGGAA CCTGATGTGT TCCTCAGGGC CCCTTTTTCC 
AGGAAACTCA GTACCGAAGA AGCATCTCAA AATATTTCAT CCCAATCATT TTCGTCTCTA 
CTTGGGCTTG ATGTGGTAGG TAATTCTACA TTCCGAAATT CTGAAATGGC AGTTGGATCC 
TCTTCAATGA AGACCTCGTA CACTACAGGC AATCTCTATT CGCATTCCAT CAGACCCTTA 
GAATCAGCTG ACTCCTCTAT TTTTACTAAT ACCTCAATGG AACCAGGCAT TATAGAGAAT 
CACATGCTTC CAGGGCACGA GGCTGCTCAA GAGACAGTAT TTGGCACGGT GGTCACAAAG 
CCTTTTCGCC CACAGTCATT GTCAAAGTAT TCCAGGCACT ATAGCCCTCA AGATGGGCGG 
AATGTGGATG CTCAACCAAT TGCTGCTTTC AAAGTCGTCT CCAAGAGCGC AAACACCTCT 
GTAGCTGGCT CTGTTCCTAT ATCCTCTGTG ACTTACAGGA CCTCAGAAAT CACTAATGTA 
GACCCATTTG GCTCTGCTCC TTTTCCTGCT AAAGCTAGCA GGCCAAAGCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821429.1
CDS	1040..4693
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGKSELEVESLIPGLEPPMPQRLSSQADSIASNRTDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965975.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965975.2

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG GAAATCAGAG 
CTGGAGGTAG AAAGCCTCAT TCCTGGGCTG GAGCCCCCAA TGCCCCAGCG ACTTTCATCC 
CAGGCAGACT CTATTGCTTC AAACCGAACA GACGATACTA ACCTGCTAAC GGGCTTTGAT 
ATCCCGGACG ATACAGAAAA AAGGGCTGAT GATGAATTTG ATCCTATTCC AGTTCTGGTA 
TCCAAAAATT CCCAAGGTGT GCATCAAGAT ATGAAGGTAT TTCCTCCTGG AAGCGATGGG 
CATATTAAAA TGCACTCAGG TGGAGACTAT CCTCTGGCTG CTGACAACAA ACACTTCCTT 
GAAATGAAAA ATGAGTGTCC ATCAAAGGGT CCATCTAGCT GCAGTTCTTT TCATAGCAGT 
GATGGAGAAG CAACTGATCA TGACCCTGAC TTGCTCGACT GCAGTGGATC AAGACCTTTA 
CTAATGGAAT CAGATGATGA TGAATGTTCT AGGAAAACTA AGGATTTAGA AGTTCCAAGT 
CCTCCTATAG ATGAAATTAG CACCCAGAAT TCTCAACTTC CTTTTCACAA TGTAAACTCT 
TGGCGGCCAG AGTTGAGCTT GGCTGAAGGT TTTGATGTAT TCTCCACTGC TCCATTCAAG 
GGTCCTAAAT TAACACAGGA TGAATCAGAA ATATTTACCA ATGCTCCCTT TGTAGCCAAG 
ACTGCATTCC CTTCATTCCA GTCACAGGAA CCTGATGTGT TCCTCAGGGC CCCTTTTTCC 
AGGAAACTCA GTACCGAAGA AGCATCTCAA AATATTTCAT CCCAATCATT TTCGTCTCTA 
CTTGGGCTTG ATGTGGTAGG TAATTCTACA TTCCGAAATT CTGAAATGGC AGTTGGATCC 
TCTTCAATGA AGACCTCGTA CACTACAGGC AATCTCTATT CGCATTCCAT CAGACCCTTA 
GAATCAGCTG ACTCCTCTAT TTTTACTAAT ACCTCAATGG AACCAGGCAT TATAGAGAAT 
CACATGCTTC CAGGGCACGA GGCTGCTCAA GAGACAGTAT TTGGCACGGT GGTCACAAAG 
CCTTTTCGCC CACAGTCATT GTCAAAGTAT TCCAGGCACT ATAGCCCTCA AGATGGGCGG 
AATGTGGATG CTCAACCAAT TGCTGCTTTC AAAGTCGTCT CCAAGAGCGC AAACACCTCT 
GTAGCTGGCT CTGTTCCTAT ATCCTCTGTG ACTTACAGGA CCTCAGAAAT CACTAATGTA 
GACCCATTTG GCTCTGCTCC TTTTCCTGCT AAAGCTAGCA GGCCAAAGCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965978.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821432.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa31260
Clone ID Related Accession (Same CDS sequence)	XM_012965978.2 , XM_012965978.3
Accession Version	XM_012965978.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGA CGATACTAAC 
CTGCTAACGG GCTTTGATAT CCCGGACGAT ACAGAAAAAA GGGCTGATGA TGAATTTGAT 
CCTATTCCAG TTCTGGTATC CAAAAATTCC CAAGGTGTGC ATCAAGATAT GAAGGTATTT 
CCTCCTGGAA GCGATGGGCA TATTAAAATG CACTCAGGTG GAGACTATCC TCTGGCTGCT 
GACAACAAAC ACTTCCTTGA AATGAAAAAT GAGTGTCCAT CAAAGGGTCC ATCTAGCTGC 
AGTTCTTTTC ATAGCAGTGA TGGAGAAGCA ACTGATCATG ACCCTGACTT GCTCGACTGC 
AGTGGATCAA GACCTTTACT AATGGAATCA GATGATGATG AATGTTCTAG GAAAACTAAG 
GATTTAGAAG TTCCAAGTCC TCCTATAGAT GAAATTAGCA CCCAGAATTC TCAACTTCCT 
TTTCACAATG TAAACTCTTG GCGGCCAGAG TTGAGCTTGG CTGAAGGTTT TGATGTATTC 
TCCACTGCTC CATTCAAGGG TCCTAAATTA ACACAGGATG AATCAGAAAT ATTTACCAAT 
GCTCCCTTTG TAGCCAAGAC TGCATTCCCT TCATTCCAGT CACAGGAACC TGATGTGTTC 
CTCAGGGCCC CTTTTTCCAG GAAACTCAGT ACCGAAGAAG CATCTCAAAA TATTTCATCC 
CAATCATTTT CGTCTCTACT TGGGCTTGAT GTGGTAGGTA ATTCTACATT CCGAAATTCT 
GAAATGGCAG TTGGATCCTC TTCAATGAAG ACCTCGTACA CTACAGGCAA TCTCTATTCG 
CATTCCATCA GACCCTTAGA ATCAGCTGAC TCCTCTATTT TTACTAATAC CTCAATGGAA 
CCAGGCATTA TAGAGAATCA CATGCTTCCA GGGCACGAGG CTGCTCAAGA GACAGTATTT 
GGCACGGTGG TCACAAAGCC TTTTCGCCCA CAGTCATTGT CAAAGTATTC CAGGCACTAT 
AGCCCTCAAG ATGGGCGGAA TGTGGATGCT CAACCAATTG CTGCTTTCAA AGTCGTCTCC 
AAGAGCGCAA ACACCTCTGT AGCTGGCTCT GTTCCTATAT CCTCTGTGAC TTACAGGACC 
TCAGAAATCA CTAATGTAGA CCCATTTGGC TCTGCTCCTT TTCCTGCTAA AGCTAGCAGG 
CCAAAGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821432.1
CDS	1039..4590
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965978.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965978.2

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGA CGATACTAAC 
CTGCTAACGG GCTTTGATAT CCCGGACGAT ACAGAAAAAA GGGCTGATGA TGAATTTGAT 
CCTATTCCAG TTCTGGTATC CAAAAATTCC CAAGGTGTGC ATCAAGATAT GAAGGTATTT 
CCTCCTGGAA GCGATGGGCA TATTAAAATG CACTCAGGTG GAGACTATCC TCTGGCTGCT 
GACAACAAAC ACTTCCTTGA AATGAAAAAT GAGTGTCCAT CAAAGGGTCC ATCTAGCTGC 
AGTTCTTTTC ATAGCAGTGA TGGAGAAGCA ACTGATCATG ACCCTGACTT GCTCGACTGC 
AGTGGATCAA GACCTTTACT AATGGAATCA GATGATGATG AATGTTCTAG GAAAACTAAG 
GATTTAGAAG TTCCAAGTCC TCCTATAGAT GAAATTAGCA CCCAGAATTC TCAACTTCCT 
TTTCACAATG TAAACTCTTG GCGGCCAGAG TTGAGCTTGG CTGAAGGTTT TGATGTATTC 
TCCACTGCTC CATTCAAGGG TCCTAAATTA ACACAGGATG AATCAGAAAT ATTTACCAAT 
GCTCCCTTTG TAGCCAAGAC TGCATTCCCT TCATTCCAGT CACAGGAACC TGATGTGTTC 
CTCAGGGCCC CTTTTTCCAG GAAACTCAGT ACCGAAGAAG CATCTCAAAA TATTTCATCC 
CAATCATTTT CGTCTCTACT TGGGCTTGAT GTGGTAGGTA ATTCTACATT CCGAAATTCT 
GAAATGGCAG TTGGATCCTC TTCAATGAAG ACCTCGTACA CTACAGGCAA TCTCTATTCG 
CATTCCATCA GACCCTTAGA ATCAGCTGAC TCCTCTATTT TTACTAATAC CTCAATGGAA 
CCAGGCATTA TAGAGAATCA CATGCTTCCA GGGCACGAGG CTGCTCAAGA GACAGTATTT 
GGCACGGTGG TCACAAAGCC TTTTCGCCCA CAGTCATTGT CAAAGTATTC CAGGCACTAT 
AGCCCTCAAG ATGGGCGGAA TGTGGATGCT CAACCAATTG CTGCTTTCAA AGTCGTCTCC 
AAGAGCGCAA ACACCTCTGT AGCTGGCTCT GTTCCTATAT CCTCTGTGAC TTACAGGACC 
TCAGAAATCA CTAATGTAGA CCCATTTGGC TCTGCTCCTT TTCCTGCTAA AGCTAGCAGG 
CCAAAGCCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965980.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821434.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X6, mRNA.

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CloneID	OXa31262
Clone ID Related Accession (Same CDS sequence)	XM_012965980.3 , XM_012965980.2
Accession Version	XM_012965980.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG TGTGCATCAA 
GATATGAAGG TATTTCCTCC TGGAAGCGAT GGGCATATTA AAATGCACTC AGGTGGAGAC 
TATCCTCTGG CTGCTGACAA CAAACACTTC CTTGAAATGA AAAATGAGTG TCCATCAAAG 
GGTCCATCTA GCTGCAGTTC TTTTCATAGC AGTGATGGAG AAGCAACTGA TCATGACCCT 
GACTTGCTCG ACTGCAGTGG ATCAAGACCT TTACTAATGG AATCAGATGA TGATGAATGT 
TCTAGGAAAA CTAAGGATTT AGAAGTTCCA AGTCCTCCTA TAGATGAAAT TAGCACCCAG 
AATTCTCAAC TTCCTTTTCA CAATGTAAAC TCTTGGCGGC CAGAGTTGAG CTTGGCTGAA 
GGTTTTGATG TATTCTCCAC TGCTCCATTC AAGGGTCCTA AATTAACACA GGATGAATCA 
GAAATATTTA CCAATGCTCC CTTTGTAGCC AAGACTGCAT TCCCTTCATT CCAGTCACAG 
GAACCTGATG TGTTCCTCAG GGCCCCTTTT TCCAGGAAAC TCAGTACCGA AGAAGCATCT 
CAAAATATTT CATCCCAATC ATTTTCGTCT CTACTTGGGC TTGATGTGGT AGGTAATTCT 
ACATTCCGAA ATTCTGAAAT GGCAGTTGGA TCCTCTTCAA TGAAGACCTC GTACACTACA 
GGCAATCTCT ATTCGCATTC CATCAGACCC TTAGAATCAG CTGACTCCTC TATTTTTACT 
AATACCTCAA TGGAACCAGG CATTATAGAG AATCACATGC TTCCAGGGCA CGAGGCTGCT 
CAAGAGACAG TATTTGGCAC GGTGGTCACA AAGCCTTTTC GCCCACAGTC ATTGTCAAAG 
TATTCCAGGC ACTATAGCCC TCAAGATGGG CGGAATGTGG ATGCTCAACC AATTGCTGCT 
TTCAAAGTCG TCTCCAAGAG CGCAAACACC TCTGTAGCTG GCTCTGTTCC TATATCCTCT 
GTGACTTACA GGACCTCAGA AATCACTAAT GTAGACCCAT TTGGCTCTGC TCCTTTTCCT 
GCTAAAGCTA GCAGGCCAAA GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821434.1
CDS	1037..4483
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965980.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965980.2

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG TGTGCATCAA 
GATATGAAGG TATTTCCTCC TGGAAGCGAT GGGCATATTA AAATGCACTC AGGTGGAGAC 
TATCCTCTGG CTGCTGACAA CAAACACTTC CTTGAAATGA AAAATGAGTG TCCATCAAAG 
GGTCCATCTA GCTGCAGTTC TTTTCATAGC AGTGATGGAG AAGCAACTGA TCATGACCCT 
GACTTGCTCG ACTGCAGTGG ATCAAGACCT TTACTAATGG AATCAGATGA TGATGAATGT 
TCTAGGAAAA CTAAGGATTT AGAAGTTCCA AGTCCTCCTA TAGATGAAAT TAGCACCCAG 
AATTCTCAAC TTCCTTTTCA CAATGTAAAC TCTTGGCGGC CAGAGTTGAG CTTGGCTGAA 
GGTTTTGATG TATTCTCCAC TGCTCCATTC AAGGGTCCTA AATTAACACA GGATGAATCA 
GAAATATTTA CCAATGCTCC CTTTGTAGCC AAGACTGCAT TCCCTTCATT CCAGTCACAG 
GAACCTGATG TGTTCCTCAG GGCCCCTTTT TCCAGGAAAC TCAGTACCGA AGAAGCATCT 
CAAAATATTT CATCCCAATC ATTTTCGTCT CTACTTGGGC TTGATGTGGT AGGTAATTCT 
ACATTCCGAA ATTCTGAAAT GGCAGTTGGA TCCTCTTCAA TGAAGACCTC GTACACTACA 
GGCAATCTCT ATTCGCATTC CATCAGACCC TTAGAATCAG CTGACTCCTC TATTTTTACT 
AATACCTCAA TGGAACCAGG CATTATAGAG AATCACATGC TTCCAGGGCA CGAGGCTGCT 
CAAGAGACAG TATTTGGCAC GGTGGTCACA AAGCCTTTTC GCCCACAGTC ATTGTCAAAG 
TATTCCAGGC ACTATAGCCC TCAAGATGGG CGGAATGTGG ATGCTCAACC AATTGCTGCT 
TTCAAAGTCG TCTCCAAGAG CGCAAACACC TCTGTAGCTG GCTCTGTTCC TATATCCTCT 
GTGACTTACA GGACCTCAGA AATCACTAAT GTAGACCCAT TTGGCTCTGC TCCTTTTCCT 
GCTAAAGCTA GCAGGCCAAA GCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965981.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821435.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa31263
Clone ID Related Accession (Same CDS sequence)	XM_012965981.3 , XM_012965981.2
Accession Version	XM_012965981.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGAC GATACTAACC TGCTAACGGG CTTTGATATC 
CCGGACGATA CAGAAAAAAG GGCTGATGAT GAATTTGATC CTATTCCAGT TCTGGTATCC 
AAAAATTCCC AAGGTGTGCA TCAAGATATG AAGGTATTTC CTCCTGGAAG CGATGGGCAT 
ATTAAAATGC ACTCAGGTGG AGACTATCCT CTGGCTGCTG ACAACAAACA CTTCCTTGAA 
ATGAAAAATG AGTGTCCATC AAAGGGTCCA TCTAGCTGCA GTTCTTTTCA TAGCAGTGAT 
GGAGAAGCAA CTGATCATGA CCCTGACTTG CTCGACTGCA GTGGATCAAG ACCTTTACTA 
ATGGAATCAG ATGATGATGA ATGTTCTAGG AAAACTAAGG ATTTAGAAGT TCCAAGTCCT 
CCTATAGATG AAATTAGCAC CCAGAATTCT CAACTTCCTT TTCACAATGT AAACTCTTGG 
CGGCCAGAGT TGAGCTTGGC TGAAGGTTTT GATGTATTCT CCACTGCTCC ATTCAAGGGT 
CCTAAATTAA CACAGGATGA ATCAGAAATA TTTACCAATG CTCCCTTTGT AGCCAAGACT 
GCATTCCCTT CATTCCAGTC ACAGGAACCT GATGTGTTCC TCAGGGCCCC TTTTTCCAGG 
AAACTCAGTA CCGAAGAAGC ATCTCAAAAT ATTTCATCCC AATCATTTTC GTCTCTACTT 
GGGCTTGATG TGGTAGGTAA TTCTACATTC CGAAATTCTG AAATGGCAGT TGGATCCTCT 
TCAATGAAGA CCTCGTACAC TACAGGCAAT CTCTATTCGC ATTCCATCAG ACCCTTAGAA 
TCAGCTGACT CCTCTATTTT TACTAATACC TCAATGGAAC CAGGCATTAT AGAGAATCAC 
ATGCTTCCAG GGCACGAGGC TGCTCAAGAG ACAGTATTTG GCACGGTGGT CACAAAGCCT 
TTTCGCCCAC AGTCATTGTC AAAGTATTCC AGGCACTATA GCCCTCAAGA TGGGCGGAAT 
GTGGATGCTC AACCAATTGC TGCTTTCAAA GTCGTCTCCA AGAGCGCAAA CACCTCTGTA 
GCTGGCTCTG TTCCTATATC CTCTGTGACT TACAGGACCT CAGAAATCAC TAATGTAGAC 
CCATTTGGCT CTGCTCCTTT TCCTGCTAAA GCTAGCAGGC CAAAGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821435.1
CDS	1036..4386
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965981.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965981.2

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGAC GATACTAACC TGCTAACGGG CTTTGATATC 
CCGGACGATA CAGAAAAAAG GGCTGATGAT GAATTTGATC CTATTCCAGT TCTGGTATCC 
AAAAATTCCC AAGGTGTGCA TCAAGATATG AAGGTATTTC CTCCTGGAAG CGATGGGCAT 
ATTAAAATGC ACTCAGGTGG AGACTATCCT CTGGCTGCTG ACAACAAACA CTTCCTTGAA 
ATGAAAAATG AGTGTCCATC AAAGGGTCCA TCTAGCTGCA GTTCTTTTCA TAGCAGTGAT 
GGAGAAGCAA CTGATCATGA CCCTGACTTG CTCGACTGCA GTGGATCAAG ACCTTTACTA 
ATGGAATCAG ATGATGATGA ATGTTCTAGG AAAACTAAGG ATTTAGAAGT TCCAAGTCCT 
CCTATAGATG AAATTAGCAC CCAGAATTCT CAACTTCCTT TTCACAATGT AAACTCTTGG 
CGGCCAGAGT TGAGCTTGGC TGAAGGTTTT GATGTATTCT CCACTGCTCC ATTCAAGGGT 
CCTAAATTAA CACAGGATGA ATCAGAAATA TTTACCAATG CTCCCTTTGT AGCCAAGACT 
GCATTCCCTT CATTCCAGTC ACAGGAACCT GATGTGTTCC TCAGGGCCCC TTTTTCCAGG 
AAACTCAGTA CCGAAGAAGC ATCTCAAAAT ATTTCATCCC AATCATTTTC GTCTCTACTT 
GGGCTTGATG TGGTAGGTAA TTCTACATTC CGAAATTCTG AAATGGCAGT TGGATCCTCT 
TCAATGAAGA CCTCGTACAC TACAGGCAAT CTCTATTCGC ATTCCATCAG ACCCTTAGAA 
TCAGCTGACT CCTCTATTTT TACTAATACC TCAATGGAAC CAGGCATTAT AGAGAATCAC 
ATGCTTCCAG GGCACGAGGC TGCTCAAGAG ACAGTATTTG GCACGGTGGT CACAAAGCCT 
TTTCGCCCAC AGTCATTGTC AAAGTATTCC AGGCACTATA GCCCTCAAGA TGGGCGGAAT 
GTGGATGCTC AACCAATTGC TGCTTTCAAA GTCGTCTCCA AGAGCGCAAA CACCTCTGTA 
GCTGGCTCTG TTCCTATATC CTCTGTGACT TACAGGACCT CAGAAATCAC TAATGTAGAC 
CCATTTGGCT CTGCTCCTTT TCCTGCTAAA GCTAGCAGGC CAAAGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965982.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821436.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa31264
Clone ID Related Accession (Same CDS sequence)	XM_012965982.3 , XM_012965982.2
Accession Version	XM_012965982.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965982.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGAA AAATTGATTG AGGGTCTCAA ATCTCCAGAG 
CCCCAGCTTC TCCTCCCTCA TCTTGTGCAG CTGGCAGATC CCTTTGGGAG CACAGCAGAG 
GATGGAAATG GTGTGCATCA AGATATGAAG GTATTTCCTC CTGGAAGCGA TGGGCATATT 
AAAATGCACT CAGGTGGAGA CTATCCTCTG GCTGCTGACA ACAAACACTT CCTTGAAATG 
AAAAATGAGT GTCCATCAAA GGGTCCATCT AGCTGCAGTT CTTTTCATAG CAGTGATGGA 
GAAGCAACTG ATCATGACCC TGACTTGCTC GACTGCAGTG GATCAAGACC TTTACTAATG 
GAATCAGATG ATGATGAATG TTCTAGGAAA ACTAAGGATT TAGAAGTTCC AAGTCCTCCT 
ATAGATGAAA TTAGCACCCA GAATTCTCAA CTTCCTTTTC ACAATGTAAA CTCTTGGCGG 
CCAGAGTTGA GCTTGGCTGA AGGTTTTGAT GTATTCTCCA CTGCTCCATT CAAGGGTCCT 
AAATTAACAC AGGATGAATC AGAAATATTT ACCAATGCTC CCTTTGTAGC CAAGACTGCA 
TTCCCTTCAT TCCAGTCACA GGAACCTGAT GTGTTCCTCA GGGCCCCTTT TTCCAGGAAA 
CTCAGTACCG AAGAAGCATC TCAAAATATT TCATCCCAAT CATTTTCGTC TCTACTTGGG 
CTTGATGTGG TAGGTAATTC TACATTCCGA AATTCTGAAA TGGCAGTTGG ATCCTCTTCA 
ATGAAGACCT CGTACACTAC AGGCAATCTC TATTCGCATT CCATCAGACC CTTAGAATCA 
GCTGACTCCT CTATTTTTAC TAATACCTCA ATGGAACCAG GCATTATAGA GAATCACATG 
CTTCCAGGGC ACGAGGCTGC TCAAGAGACA GTATTTGGCA CGGTGGTCAC AAAGCCTTTT 
CGCCCACAGT CATTGTCAAA GTATTCCAGG CACTATAGCC CTCAAGATGG GCGGAATGTG 
GATGCTCAAC CAATTGCTGC TTTCAAAGTC GTCTCCAAGA GCGCAAACAC CTCTGTAGCT 
GGCTCTGTTC CTATATCCTC TGTGACTTAC AGGACCTCAG AAATCACTAA TGTAGACCCA 
TTTGGCTCTG CTCCTTTTCC TGCTAAAGCT AGCAGGCCAA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821436.1
CDS	1035..4382
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGVHQDMKVFPPGSDGHIKMHSGGDYPLAADNKHFLEMKNECPSKGPSSCSSFHSSDGEATDHDPDLLDCSGSRPLLMESDDDECSRKTKDLEVPSPPIDEISTQNSQLPFHNVNSWRPELSLAEGFDVFSTAPFKGPKLTQDESEIFTNAPFVAKTAFPSFQSQEPDVFLRAPFSRKLSTEEASQNISSQSFSSLLGLDVVGNSTFRNSEMAVGSSSMKTSYTTGNLYSHSIRPLESADSSIFTNTSMEPGIIENHMLPGHEAAQETVFGTVVTKPFRPQSLSKYSRHYSPQDGRNVDAQPIAAFKVVSKSANTSVAGSVPISSVTYRTSEITNVDPFGSAPFPAKASRPKP

Target ORF information:

RefSeq Version	XM_012965982.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965982.2

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGAA AAATTGATTG AGGGTCTCAA ATCTCCAGAG 
CCCCAGCTTC TCCTCCCTCA TCTTGTGCAG CTGGCAGATC CCTTTGGGAG CACAGCAGAG 
GATGGAAATG GTGTGCATCA AGATATGAAG GTATTTCCTC CTGGAAGCGA TGGGCATATT 
AAAATGCACT CAGGTGGAGA CTATCCTCTG GCTGCTGACA ACAAACACTT CCTTGAAATG 
AAAAATGAGT GTCCATCAAA GGGTCCATCT AGCTGCAGTT CTTTTCATAG CAGTGATGGA 
GAAGCAACTG ATCATGACCC TGACTTGCTC GACTGCAGTG GATCAAGACC TTTACTAATG 
GAATCAGATG ATGATGAATG TTCTAGGAAA ACTAAGGATT TAGAAGTTCC AAGTCCTCCT 
ATAGATGAAA TTAGCACCCA GAATTCTCAA CTTCCTTTTC ACAATGTAAA CTCTTGGCGG 
CCAGAGTTGA GCTTGGCTGA AGGTTTTGAT GTATTCTCCA CTGCTCCATT CAAGGGTCCT 
AAATTAACAC AGGATGAATC AGAAATATTT ACCAATGCTC CCTTTGTAGC CAAGACTGCA 
TTCCCTTCAT TCCAGTCACA GGAACCTGAT GTGTTCCTCA GGGCCCCTTT TTCCAGGAAA 
CTCAGTACCG AAGAAGCATC TCAAAATATT TCATCCCAAT CATTTTCGTC TCTACTTGGG 
CTTGATGTGG TAGGTAATTC TACATTCCGA AATTCTGAAA TGGCAGTTGG ATCCTCTTCA 
ATGAAGACCT CGTACACTAC AGGCAATCTC TATTCGCATT CCATCAGACC CTTAGAATCA 
GCTGACTCCT CTATTTTTAC TAATACCTCA ATGGAACCAG GCATTATAGA GAATCACATG 
CTTCCAGGGC ACGAGGCTGC TCAAGAGACA GTATTTGGCA CGGTGGTCAC AAAGCCTTTT 
CGCCCACAGT CATTGTCAAA GTATTCCAGG CACTATAGCC CTCAAGATGG GCGGAATGTG 
GATGCTCAAC CAATTGCTGC TTTCAAAGTC GTCTCCAAGA GCGCAAACAC CTCTGTAGCT 
GGCTCTGTTC CTATATCCTC TGTGACTTAC AGGACCTCAG AAATCACTAA TGTAGACCCA 
TTTGGCTCTG CTCCTTTTCC TGCTAAAGCT AGCAGGCCAA AGCCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965983.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821437.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OXa31265
Clone ID Related Accession (Same CDS sequence)	XM_012965983.2 , XM_012965983.3
Accession Version	XM_012965983.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965983.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG GAAATCAGAG 
CTGGAGGTAG AAAGCCTCAT TCCTGGGCTG GAGCCCCCAA TGCCCCAGCG ACTTTCATCC 
CAGGCAGACT CTATTGCTTC AAACCGAACA GACTCCCTCA CTGGGGAAGA TTCTCTCCTT 
GACTACTCTC TTCTCTCTAA CCCCGCGGCT GATCTTCTTG ACGAGTTTGC TCCTGTAACA 
CATTCGGCTC TGGCTTCCAC AGACGATACT AACCTGCTAA CGGGCTTTGA TATCCCGGAC 
GATACAGAAA AAAGGGCTGA TGATGAATTT GATCCTATTC CAGTTCTGGT ATCCAAAAAT 
TCCCAAGGTA CTCACTCTAG TAGCAGCAGT GGGAATTCTG AGTCGAGCCT ACCAAACATG 
ACCATGTCTC TTCTACTGGT GGATCAGCTC ATAGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821437.1
CDS	1035..3836
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGKSELEVESLIPGLEPPMPQRLSSQADSIASNRTDSLTGEDSLLDYSLLSNPAADLLDEFAPVTHSALASTDDTNLLTGFDIPDDTEKRADDEFDPIPVLVSKNSQGTHSSSSSGNSESSLPNMTMSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_012965983.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965983.2

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG GAAATCAGAG 
CTGGAGGTAG AAAGCCTCAT TCCTGGGCTG GAGCCCCCAA TGCCCCAGCG ACTTTCATCC 
CAGGCAGACT CTATTGCTTC AAACCGAACA GACTCCCTCA CTGGGGAAGA TTCTCTCCTT 
GACTACTCTC TTCTCTCTAA CCCCGCGGCT GATCTTCTTG ACGAGTTTGC TCCTGTAACA 
CATTCGGCTC TGGCTTCCAC AGACGATACT AACCTGCTAA CGGGCTTTGA TATCCCGGAC 
GATACAGAAA AAAGGGCTGA TGATGAATTT GATCCTATTC CAGTTCTGGT ATCCAAAAAT 
TCCCAAGGTA CTCACTCTAG TAGCAGCAGT GGGAATTCTG AGTCGAGCCT ACCAAACATG 
ACCATGTCTC TTCTACTGGT GGATCAGCTC ATAGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965984.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821438.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OXa31266
Clone ID Related Accession (Same CDS sequence)	XM_012965984.2 , XM_012965984.3
Accession Version	XM_012965984.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG TACTCACTCT 
AGTAGCAGCA GTGGGAATTC TGAGTCGAGC CTACCAAACA TGACCATGTC TCTTCTACTG 
GTGGATCAGC TCATAGACTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821438.1
CDS	1030..3513
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSAADKPVDSQGPSPGSQLQKTSDSFLPLPLSDTPEKLIEGLKSPEPQLLLPHLVQLADPFGSTAEDGNGTHSSSSSGNSESSLPNMTMSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_012965984.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965984.2

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGCT GCAGATAAAC CTGTAGATTC ACAGGGGCCC 
AGTCCTGGCT CTCAGCTGCA AAAAACTTCT GATTCTTTCT TGCCTCTCCC TCTGTCTGAC 
ACCCCAGAAA AATTGATTGA GGGTCTCAAA TCTCCAGAGC CCCAGCTTCT CCTCCCTCAT 
CTTGTGCAGC TGGCAGATCC CTTTGGGAGC ACAGCAGAGG ATGGAAATGG TACTCACTCT 
AGTAGCAGCA GTGGGAATTC TGAGTCGAGC CTACCAAACA TGACCATGTC TCTTCTACTG 
GTGGATCAGC TCATAGACTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aak1 ( XM_012965985.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821439.1 Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X11, mRNA.

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CloneID	OXa31267
Clone ID Related Accession (Same CDS sequence)	XM_012965985.3 , XM_012965985.2
Accession Version	XM_012965985.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	AP2-associated protein kinase 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965985.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGGT ACTCACTCTA GTAGCAGCAG TGGGAATTCT 
GAGTCGAGCC TACCAAACAT GACCATGTCT CTTCTACTGG TGGATCAGCT CATAGACTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821439.1
CDS	1025..3307
Translation	MKKFFDSRRELVGSGSGVGGGSSSGLGTGYIGRVFTIGRHQVTVDEVLAEGGFALVFLVRTSNGMRRALKRMYVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDYCRGGQVVNLMNQRLQTGFTESEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKCQNPQTEGVTAVEDEIKKYTTLSYRAPEMVNLYSGKMITTKADIWALGCLLYKLCFFTLPFGESQVSICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLAKRECPVQNVHDAVIPVKLPEPIKAGEAAAKKTQPKARLTDPVPTTETSIAPRQRPKPGQPQPNPGMLPIQPALTPRKRAMAQPANPSPVAPVSIQSPNPAAVAPAVRPSVQTQPKAAQPAAQVPTPVTTTPQPATPQAPPAAVQSALTQQQQQLYLKQQLMQQQQQQAAAYYQQQQQQQMLQAQQIQAMQQQQMLQAYYMQQQHLLAQHAAMQQKVATPKQSSLPSQPQAMPAALQPPAAQEQGQPAPLKTPQKPQPPTAPPSSAPPTAAGQKLGSLTPPSSPKTQRGGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEANLNKSKSATTTPSGSPRTSQQNVHNPPEPSGWNPFDDDNFSKLTAEELLNKDFAKLGESKPSEQLSRSAEDLLSGFPAPPAVSQADSFGTALFASGSGTHSSSSSGNSESSLPNMTMSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_012965985.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis AP2 associated kinase 1 (aak1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965985.2

ORF Insert Sequence:

ATGAAGAAGT TTTTTGACTC CCGGCGTGAG CTGGTGGGGT CCGGTTCTGG AGTCGGTGGT 
GGCTCGTCCA GCGGCCTGGG GACTGGCTAC ATCGGCAGAG TCTTTACTAT TGGCAGGCAT 
CAGGTCACCG TGGATGAGGT CCTGGCTGAA GGTGGATTTG CACTTGTGTT TCTCGTTCGC 
ACAAGCAATG GGATGCGCCG AGCACTTAAA CGCATGTATG TGAACAATGA GCATGACCTA 
CAGGTGTGCA AGAGGGAAAT TCAGATCATG AGGGATCTCT CCGGGCACAA GAACATTGTT 
GGATACATAG ATTCCAGTAT TAATTCTGTG AGCAGTGGAG ATGTGTGGGA AGTTCTGATC 
CTAATGGATT ACTGTCGAGG AGGGCAAGTT GTGAATCTAA TGAACCAACG GCTGCAAACT 
GGTTTCACAG AAAGTGAAGT TCTCCAGATC TTCTGTGACA CATGTGAGGC AGTGGCTCGT 
CTCCATCAGT GCAAGACTCC TATTATTCAT CGAGATCTCA AGGTTGAGAA TATTTTACTA 
CATGACCGTG GTCACTATGT GCTGTGTGAC TTTGGAAGTG CAACAAATAA ATGCCAGAAT 
CCACAGACAG AAGGCGTCAC TGCAGTGGAA GATGAGATTA AAAAGTACAC AACCCTCTCA 
TATCGGGCTC CTGAGATGGT GAATCTGTAC AGTGGCAAAA TGATCACCAC CAAAGCGGAC 
ATCTGGGCCT TAGGCTGCTT GTTGTACAAG CTCTGCTTCT TCACACTTCC ATTTGGAGAG 
AGTCAGGTGT CCATTTGTGA TGGGAATTTC ACTATCCCAG ACAACTCTCG CTACTCCCAG 
GACATGCACT GCTTGATCCG TTACATGCTT GAGCCAGACC CTGACAAGAG ACCCGATATC 
TACCAAGTAT CTTATTTCTC CTTCAAGCTG GCAAAAAGGG AGTGCCCTGT GCAGAATGTG 
CATGATGCTG TGATTCCTGT AAAGCTTCCA GAACCTATAA AAGCTGGGGA GGCTGCTGCT 
AAGAAAACCC AACCAAAGGC CAGGTTGACA GACCCTGTTC CAACAACAGA GACATCCATA 
GCGCCTCGCC AGAGGCCGAA ACCTGGTCAG CCTCAGCCAA ACCCTGGGAT GCTTCCAATT 
CAGCCAGCTT TAACCCCACG CAAAAGAGCC ATGGCTCAGC CAGCCAATCC CTCTCCAGTT 
GCACCAGTAA GTATCCAGTC CCCTAACCCT GCAGCTGTGG CACCAGCCGT GAGACCATCT 
GTACAAACTC AGCCAAAAGC AGCACAGCCA GCGGCACAGG TTCCCACACC TGTAACCACC 
ACTCCACAGC CCGCTACTCC ACAGGCTCCT CCAGCTGCTG TGCAATCTGC ACTGACACAA 
CAGCAACAGC AGCTGTACTT GAAACAGCAA CTTATGCAAC AACAACAGCA GCAGGCTGCG 
GCATATTATC AACAGCAGCA GCAGCAGCAG ATGCTGCAGG CTCAACAGAT CCAGGCCATG 
CAACAGCAGC AGATGCTTCA GGCCTATTAC ATGCAGCAGC AACATCTATT GGCACAGCAT 
GCCGCTATGC AACAGAAAGT GGCAACACCT AAACAGTCTT CTTTACCAAG TCAGCCACAA 
GCCATGCCAG CTGCTCTCCA GCCTCCTGCA GCTCAGGAAC AGGGGCAGCC AGCTCCACTC 
AAGACCCCAC AGAAGCCCCA GCCACCTACA GCACCTCCAT CTTCTGCACC TCCCACAGCA 
GCAGGGCAGA AACTGGGATC CCTTACCCCT CCTTCATCCC CCAAGACCCA AAGAGGCGGC 
CACAGAAGGA TACTTAGTGA TGTTACGCAT AGTGCAGTTT TCGGTGTCCC AGCCAGTAAA 
TCTACTCAGC TTCTACAGGC TGCTGCGGCT GAAGCAAACC TCAACAAATC AAAGTCAGCT 
ACAACCACCC CTTCTGGCTC ACCTCGGACC TCCCAGCAGA ATGTTCACAA TCCGCCAGAA 
CCTTCTGGCT GGAATCCTTT CGACGATGAC AACTTTTCCA AACTCACAGC TGAGGAACTT 
CTAAATAAGG ATTTTGCCAA GCTTGGAGAA AGTAAGCCAT CGGAGCAGCT GAGCCGCTCA 
GCAGAAGATC TCCTATCTGG GTTTCCAGCT CCACCTGCTG TATCTCAGGC TGATTCATTT 
GGAACTGCAC TCTTTGCCAG TGGTTCAGGT ACTCACTCTA GTAGCAGCAG TGGGAATTCT 
GAGTCGAGCC TACCAAACAT GACCATGTCT CTTCTACTGG TGGATCAGCT CATAGACTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	aak1
Entrez Gene ID	100135375
Full Name	AP2 associated kinase 1
General protein information	Preferred Names AP2 associated kinase 1 Names AP2-associated protein kinase 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Danio rerio (zebrafish)	si:dkey-71c4.3	XP_005167372.1
Xenopus tropicalis (tropical clawed frog)	aak1	NP_001107518.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

aak1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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