nbn.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nbn.L
Entrez Gene ID	403373
Full Name	nibrin L homeolog
Synonyms	nbn,nbs,nbs1
General protein information	Preferred Names nibrin L homeolog Names nibrin L homeolog Nijmegen breakage syndrome 1
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001090951.1	NP_001084420.1	nibrin L homeolog

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mdc1 modulates the interaction between TopBP1 and the MRN complex during DNA damage checkpoint responses.
Choi SH, Yoo HY
Biochemical and biophysical research communications479(1)5-11(2016 10)

MRN, CtIP, and BRCA1 mediate repair of topoisomerase II-DNA adducts.
Aparicio T, Baer R, Gottesman M, Gautier J
The Journal of cell biology212(4)399-408(2016 Feb)

A role for the MRN complex in ATR activation via TOPBP1 recruitment.
Duursma AM, Driscoll R, Elias JE, Cimprich KA
Molecular cell50(1)116-22(2013 Apr)

Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM.
Wawrousek KE, Fortini BK, Polaczek P, Chen L, Liu Q, Dunphy WG, Campbell JL
Cell cycle (Georgetown, Tex.)9(6)1156-66(2010 Mar)

The Mre11/Rad50/Nbs1 complex functions in resection-based DNA end joining in Xenopus laevis.
Taylor EM, Cecillon SM, Bonis A, Chapman JR, Povirk LF, Lindsay HD
Nucleic acids research38(2)441-54(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that TopBP1 and Nbs1 associate with the N-terminal region of Mdc1 in egg extracts.
Title: Mdc1 modulates the interaction between TopBP1 and the MRN complex during DNA damage checkpoint responses.

MRN (Mre11, Rad50, and Nbs1) complex, CtIP, and BRCA1 are required for both the removal of Top2-DNA adducts and the subsequent resection of Top2-adducted DSB ends.
Title: MRN, CtIP, and BRCA1 mediate repair of topoisomerase II-DNA adducts.

MRN (MRE11-RAD50-NBS1) complex has role in ATR activation via TOPBP1 recruitment.
Title: A role for the MRN complex in ATR activation via TOPBP1 recruitment.

Dna2 co-localizes in foci with RPA and is found in a complex with replication fork components And-1 and Mcm10. Dna2 interacts with the DSB repair and checkpoint proteins Nbs1 and ATM.
Title: Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM.

Results indicate a role for the X. laevis Mre11/Rad50/Nbs1 complex in microhomology-mediated end joining.
Title: The Mre11/Rad50/Nbs1 complex functions in resection-based DNA end joining in Xenopus laevis.

The following nbn.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nbn.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf01419	NM_001090951.1 Latest version!	Xenopus laevis nibrin L homeolog (nbn.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nbn.L ( NM_001090951.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001084420.1 Xenopus laevis nibrin L homeolog (nbn.L), mRNA.

Price & Availability↑

CloneID	OAf01419
Clone ID Related Accession (Same CDS sequence)	NM_001090951.1
Accession Version	NM_001090951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-25
Organism	Xenopus laevis(African clawed frog)
Product	nibrin L homeolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY207367.1. On Jul 25, 2007 this sequence version replaced NM_001090952.1. ##Evidence-Data-START## Transcript exon combination :: AY207367.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00012418, SAMD00012419 [ECO:0000350] ##Evidence-Data-END##

ATGTGGAGGC TGGTGGCAGA GAGCGCTGCA GGAGGCACGT ATCATTTTCT CACTGGCACC 
GATTATGTTG TGGGGCGCAA GAACTGTGCT ATTTTAATTC CAGAAGATCA GTCCATTAGT 
AGGTGCCATG CTACGTTGTC TGTTAGCCAC CCAAGTGCCA ATCTGGGCCA AACAAATGCC 
GCTTCAGTCC TATCGATAAA AGACTCATCA AAGTATGGCA CAACCGTCAA TGGAGACAAG 
ATGAATCCTG CGGTTCCAAG GAATTTGAAA TCCGGAGATA AGGTCACATT CGGTGTTTTT 
AATAGCAAGT ATAGGGTGGA ATACGAGCCC TTAGTGGTCT GTTCTTCCTG TTTAGATAAC 
TCGGAGAAGA ATTCTTTAAA CCAGAACCTC ATACATTTAG GTGGCCATGT GTTGAATAAC 
TGGACAGAGA AGAGCACTCA CCTTGTTATG ACTTCTATTA AAGTAACTAT CAAGACTATA 
TGTGCACTCA TTTGCTGCAA ACCAATCATA AAACCAGACT ACTTCTGTGA GCTGCTTCAT 
GCCATTCAGG AGAAAAGACC ATTGCCTGAT TATAGAAGTT TTATACCTTC TGTAGATGAG 
CCATCACTTA CACCCGAATC ACTAGATCTT TCCGAAAATG TGAAACGCAA ATCCATATTC 
AAGGATAAAG TATTCCTGTT TCTCAATGCG AAACAGTATA AGAAGCTGAG CCCAGCAGTT 
CTGTTTGGTG GGGGAAAGAC AGATCTGCTG ATGGGGGAAC TGAAAGACGC ATCAGTACTG 
GATAATCCGG CAACATGTGT TATTGACGTG GCCATGACTG AATCTCAGCT CTCCGAATCC 
CAGTCTACAC AGCCCTGGAT CACTTCTACA CTGGATTTAC TGCAAAGTAA AGGTCTGAGA 
ACAATTCCAG AAGCAGAAAT TGGTTTGGCT GTTATCAATG TGTCAACAGA AATTTATTGC 
AATCCTCGAA GACGTGCAGC AAGTGGAACT GAGGCGGGAA CATCGAAGAA AATGAATGTC 
CTGAGTTCTA CCCTTTGTCA AGGTATTGCT GTTGATGAGA CCATATTACC TGCTCCAACA 
CTTGATATAA CGGCTTATGC TGCCAATACA GAACCGCAAG ATCAGACAGG TACATCATGG 
ATGAACATCA GCGGTGTTCG TGAAGTGAAA GAAACCCCGG GTAACAGTAG CAGCCACAGT 
AGATCCAAAG GTTGCCTACA GAAGGATCCA AAAAGTGGCA GCAGCAGCAA TGATCTCAGA 
CAGACTTTAT TCCGAGAGGA TGAAACCGAC ACCAGGAAGA ATACTCCATC GCTCTTACCC 
ACAAAATCTT CTGCTCGAGA TGTGACGAAG ACTTCCCAAA AACTGCAGCC AACTAAAAAA 
ATTGACAGTT ACTTTCAATC GTTGGCCAAG AAAAGAGACC GGGCTGAAGA TGAAAAGGAA 
GCATCTTCAT CAAAGTTGCC ACGGGTGGAA ACCCTTTCAT CCCAAACTTT AGAGGAGAAA 
TTCCCGCCAG TTACACAAAC ACTTGAGGAA GAGAATTTTG ACTCCGATCT GGATATGGAA 
TTGGCAATGT CATGTGATCA GATACTAAAT GATTCCATGG GGAGCAAAGC AGCGATGCCA 
GAAGATTCAA CGGTGAAAAA AAGAAAGGTG CCCGATGATC ATGTAGTGGA AGAGTCTGAC 
GTCGATAGCG ATGAAGGAAT CCGTGCGAAT GCAGAGCAGA ATGATACTAA AAGTGATATA 
AATGTGACAA AAAGAAGAAA AGTTGATTCT GAAATAGAAG GCACCAAAGA GCCCACTATA 
AAGCCACGTG AGATAGGAAC AAAGGCACAA GTGAAGACGA ATTCTCCAGA GATGGCTCCG 
GCAATAAGGA AAGAGATTGA GGTCAAGAAA GAGTTGGATA TCAAGAAAGA GCCTAAATCT 
CAATGGGAAG AATCTAAGTT TGTTCCTGAT CTCCAAGGGG AAACTGATGC GCTGCCCAGC 
AGACTCTTAC TTACTGAGTT TAAGTCATTG GTGGTGAGCC GGCCAGTCAG GAACAATAAC 
GCCACAAGCA ATAGTAGCAA CGGAAAGTGC CATAACTTTA AGAAATTCAA AAAGGTAGCG 
TACCCAGGCG CTGGAAGCTT TCCCAATATT ATTGGAGGGT CTGATTTAAT TGCGCATGAT 
AGAAAGAAAA ATGCAGAGCT GGAACAGTGG CTTCGCCAGG AAGTGGAGGA ACAAACGCAA 
CAAGTCAGAG AAGAGTCACT TGCTGAGGAT TTGTTCAGGT ACAATCCCAA ACCTAGCAAA 
AGAAGACGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001084420.1
CDS	66..2357
Translation	MWRLVAESAAGGTYHFLTGTDYVVGRKNCAILIPEDQSISRCHATLSVSHPSANLGQTNAASVLSIKDSSKYGTTVNGDKMNPAVPRNLKSGDKVTFGVFNSKYRVEYEPLVVCSSCLDNSEKNSLNQNLIHLGGHVLNNWTEKSTHLVMTSIKVTIKTICALICCKPIIKPDYFCELLHAIQEKRPLPDYRSFIPSVDEPSLTPESLDLSENVKRKSIFKDKVFLFLNAKQYKKLSPAVLFGGGKTDLLMGELKDASVLDNPATCVIDVAMTESQLSESQSTQPWITSTLDLLQSKGLRTIPEAEIGLAVINVSTEIYCNPRRRAASGTEAGTSKKMNVLSSTLCQGIAVDETILPAPTLDITAYAANTEPQDQTGTSWMNISGVREVKETPGNSSSHSRSKGCLQKDPKSGSSSNDLRQTLFREDETDTRKNTPSLLPTKSSARDVTKTSQKLQPTKKIDSYFQSLAKKRDRAEDEKEASSSKLPRVETLSSQTLEEKFPPVTQTLEEENFDSDLDMELAMSCDQILNDSMGSKAAMPEDSTVKKRKVPDDHVVEESDVDSDEGIRANAEQNDTKSDINVTKRRKVDSEIEGTKEPTIKPREIGTKAQVKTNSPEMAPAIRKEIEVKKELDIKKEPKSQWEESKFVPDLQGETDALPSRLLLTEFKSLVVSRPVRNNNATSNSSNGKCHNFKKFKKVAYPGAGSFPNIIGGSDLIAHDRKKNAELEQWLRQEVEEQTQQVREESLAEDLFRYNPKPSKRRR

Target ORF information:

RefSeq Version	NM_001090951.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis nibrin L homeolog (nbn.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001090951.1

ORF Insert Sequence:

ATGTGGAGGC TGGTGGCAGA GAGCGCTGCA GGAGGCACGT ATCATTTTCT CACTGGCACC 
GATTATGTTG TGGGGCGCAA GAACTGTGCT ATTTTAATTC CAGAAGATCA GTCCATTAGT 
AGGTGCCATG CTACGTTGTC TGTTAGCCAC CCAAGTGCCA ATCTGGGCCA AACAAATGCC 
GCTTCAGTCC TATCGATAAA AGACTCATCA AAGTATGGCA CAACCGTCAA TGGAGACAAG 
ATGAATCCTG CGGTTCCAAG GAATTTGAAA TCCGGAGATA AGGTCACATT CGGTGTTTTT 
AATAGCAAGT ATAGGGTGGA ATACGAGCCC TTAGTGGTCT GTTCTTCCTG TTTAGATAAC 
TCGGAGAAGA ATTCTTTAAA CCAGAACCTC ATACATTTAG GTGGCCATGT GTTGAATAAC 
TGGACAGAGA AGAGCACTCA CCTTGTTATG ACTTCTATTA AAGTAACTAT CAAGACTATA 
TGTGCACTCA TTTGCTGCAA ACCAATCATA AAACCAGACT ACTTCTGTGA GCTGCTTCAT 
GCCATTCAGG AGAAAAGACC ATTGCCTGAT TATAGAAGTT TTATACCTTC TGTAGATGAG 
CCATCACTTA CACCCGAATC ACTAGATCTT TCCGAAAATG TGAAACGCAA ATCCATATTC 
AAGGATAAAG TATTCCTGTT TCTCAATGCG AAACAGTATA AGAAGCTGAG CCCAGCAGTT 
CTGTTTGGTG GGGGAAAGAC AGATCTGCTG ATGGGGGAAC TGAAAGACGC ATCAGTACTG 
GATAATCCGG CAACATGTGT TATTGACGTG GCCATGACTG AATCTCAGCT CTCCGAATCC 
CAGTCTACAC AGCCCTGGAT CACTTCTACA CTGGATTTAC TGCAAAGTAA AGGTCTGAGA 
ACAATTCCAG AAGCAGAAAT TGGTTTGGCT GTTATCAATG TGTCAACAGA AATTTATTGC 
AATCCTCGAA GACGTGCAGC AAGTGGAACT GAGGCGGGAA CATCGAAGAA AATGAATGTC 
CTGAGTTCTA CCCTTTGTCA AGGTATTGCT GTTGATGAGA CCATATTACC TGCTCCAACA 
CTTGATATAA CGGCTTATGC TGCCAATACA GAACCGCAAG ATCAGACAGG TACATCATGG 
ATGAACATCA GCGGTGTTCG TGAAGTGAAA GAAACCCCGG GTAACAGTAG CAGCCACAGT 
AGATCCAAAG GTTGCCTACA GAAGGATCCA AAAAGTGGCA GCAGCAGCAA TGATCTCAGA 
CAGACTTTAT TCCGAGAGGA TGAAACCGAC ACCAGGAAGA ATACTCCATC GCTCTTACCC 
ACAAAATCTT CTGCTCGAGA TGTGACGAAG ACTTCCCAAA AACTGCAGCC AACTAAAAAA 
ATTGACAGTT ACTTTCAATC GTTGGCCAAG AAAAGAGACC GGGCTGAAGA TGAAAAGGAA 
GCATCTTCAT CAAAGTTGCC ACGGGTGGAA ACCCTTTCAT CCCAAACTTT AGAGGAGAAA 
TTCCCGCCAG TTACACAAAC ACTTGAGGAA GAGAATTTTG ACTCCGATCT GGATATGGAA 
TTGGCAATGT CATGTGATCA GATACTAAAT GATTCCATGG GGAGCAAAGC AGCGATGCCA 
GAAGATTCAA CGGTGAAAAA AAGAAAGGTG CCCGATGATC ATGTAGTGGA AGAGTCTGAC 
GTCGATAGCG ATGAAGGAAT CCGTGCGAAT GCAGAGCAGA ATGATACTAA AAGTGATATA 
AATGTGACAA AAAGAAGAAA AGTTGATTCT GAAATAGAAG GCACCAAAGA GCCCACTATA 
AAGCCACGTG AGATAGGAAC AAAGGCACAA GTGAAGACGA ATTCTCCAGA GATGGCTCCG 
GCAATAAGGA AAGAGATTGA GGTCAAGAAA GAGTTGGATA TCAAGAAAGA GCCTAAATCT 
CAATGGGAAG AATCTAAGTT TGTTCCTGAT CTCCAAGGGG AAACTGATGC GCTGCCCAGC 
AGACTCTTAC TTACTGAGTT TAAGTCATTG GTGGTGAGCC GGCCAGTCAG GAACAATAAC 
GCCACAAGCA ATAGTAGCAA CGGAAAGTGC CATAACTTTA AGAAATTCAA AAAGGTAGCG 
TACCCAGGCG CTGGAAGCTT TCCCAATATT ATTGGAGGGT CTGATTTAAT TGCGCATGAT 
AGAAAGAAAA ATGCAGAGCT GGAACAGTGG CTTCGCCAGG AAGTGGAGGA ACAAACGCAA 
CAAGTCAGAG AAGAGTCACT TGCTGAGGAT TTGTTCAGGT ACAATCCCAA ACCTAGCAAA 
AGAAGACGCT GA