mcm4.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	mcm4.L
Entrez Gene ID	397843
Full Name	minichromosome maintenance complex component 4 L homeolog
Synonyms	cdc21,mcm4,mcm4-a,mcm4-b,mcm4.S
General protein information	Preferred Names minichromosome maintenance complex component 4 L homeolog Names DNA replication licensing factor mcm4-B CDC21 homolog-B P1-CDC21-B minichromosome maintenance complex component 4 S homeolog minichromosome maintenance protein 4-B xMCM4-B
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001087979.1	NP_001081448.1	DNA replication licensing factor mcm4-B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The ATPase activity of MCM2-7 is dispensable for pre-RC assembly but is required for DNA unwinding.
Ying CY, Gautier J
The EMBO journal24(24)4334-44(2005 Dec)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Distinct phosphoisoforms of the Xenopus Mcm4 protein regulate the function of the Mcm complex.
Pereverzeva I, Whitmire E, Khan B, Cou? M
Molecular and cellular biology20(10)3667-76(2000 May)

Evidence for different MCM subcomplexes with differential binding to chromatin in Xenopus.
Cou? M, Amariglio F, Maiorano D, Bocquet S, M?chali M
Experimental cell research245(2)282-9(1998 Dec)

Developmental regulation of MCM replication factors in Xenopus laevis.
Sible JC, Erikson E, Hendrickson M, Maller JL, Gautier J
Current biology : CB8(6)347-50(1998 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mcm4.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mcm4.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf00856	NM_001087979.1 Latest version!	Xenopus laevis minichromosome maintenance complex component 4 L homeolog (mcm4.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mcm4.L ( NM_001087979.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001081448.1 Xenopus laevis minichromosome maintenance complex component 4 L homeolog (mcm4.L), mRNA.

Price & Availability↑

CloneID	OAf00856
Clone ID Related Accession (Same CDS sequence)	NM_001087979.1
Accession Version	NM_001087979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Xenopus laevis(African clawed frog)
Product	DNA replication licensing factor mcm4-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U29178.1. On Jul 25, 2007 this sequence version replaced NM_001087980.1. ##Evidence-Data-START## Transcript exon combination :: U29178.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012418, SAMD00012419 [ECO:0000348] ##Evidence-Data-END##

ATGTCCTCTC CAACATCCAC ACCAAGCCGC AGGAGGAACA AACGTGGGCG AGGCAGTAAC 
CCTCCAACTC CTCATGGAGA GGAAGTGCAG TCTCCTCCGT CACAGAGACG ACGGACAGAA 
GATTCCACAT CAATTGGTGA ACTACTGCCC ATGCCAACCT CTCCTTCAGG TGACGTTCAG 
AGCCCCAGTG GACAAGAGCT CTTGTTTTCT AGCCCTGTAC CATCAAGACA TTCAGCCCAT 
CAAAGTGAGC TTGATCTTAG TTCTCCTCTG ACTTATGGCA CCCCTAGCTC AAGGGTTGAG 
GGTACCCCAA GAAGTGGTAT CCGAGGGACA CCTGCTAGAC AGAGACCTGA CTTGGGATCA 
GCACGGAAAG TTAAGCAAGT GGATTTACAT TCAGACCAGC CTGCTGCAGA GGAGCTAGTT 
ACGAGTGAGC AATCTTTGGG ACAGAAGTTG GTGATCTGGG GAACTGATGT CAATGTAGCC 
ACTTGCAAAG AAAAGTTTCA GCGTTTTGTT CAGCGTTTCA TTGATCCTTC AGCCAAAGAA 
GAGGATAATG TAGGACTGGA TCTGAATGAG CCTATATATA TGCAGCGACT TGAAGAGATA 
AATGTGGTAG GTGATCCATT TCTCAACATT GACTGTGACC ATCTCAGAAA CTTTGACCAG 
GATCTTTACA GGCAGCTAGT GTGCTACCCA CAGGAAGTCA TTCCAACATT TGATATGGCT 
GCTAATGAGA TTTTCTTTGA GCGGTATCCT GACTCCATTT TGGAGCACCA GATTCAAGTT 
CGACCCTATA ATGCATTGAA AACTAGGAAT ATGAGAAGCC TAAATCCTGA AGATATTGAT 
CAACTTATCA CAATTAGTGG CATGGTTATC AGAACTTCAC AAATCATCCC TGAGATGCAG 
GAAGCCTTTT TTAAATGTCA AGTGTGTGCC TTCACTACTC GGGTGGAGAT TGATCGTGGT 
CGGATTGCAG AGCCATCGGT CTGCAAACAC TGTAACACCA CACACAGCAT GGCTCTTATT 
CACAATCGCT CTATGTTTTC AGACAAGCAA ATGATTAAAC TGCAAGAGTC TCCAGAAGAT 
ATGCCTGCTG GTCAGACCCC TCATACAACT ATCCTCTATG GTCATAATGA CTTGGTGGAT 
AAGGTACAGC CTGGTGATAG AGTGAATGTC ACAGGTATTT ATAGAGCTGT TCCAATTCGA 
GTTAACCCTA GAGTTCGAAA TGTGAAGTCT GTTTACAAAA CTCACATCGA TGTAATTCAT 
TACCGAAAAA CTGATTCAAA GCGGTTGCAT GGTATTGATG AAGACACTGA ACAGAAGTTG 
TTCACTGAAG AAAGGGTTGC AATGTTAAAG GAACTGGCAG CAAAGCCAGA CATCTATGAG 
AGGCTTGCTG CAGCTCTTGC ACCAAGTATA TATGAGCATG AAGATATAAA AAAGGGAATT 
TTACTTCAGT TGTTTGGTGG GACACGGAAG GACTTCAGCC ATACCGGAAG GGGCAAATTC 
CGAGCTGAAG TAAACATACT TTTGTGTGGT GATCCAGGAA CGAGTAAATC TCAGCTTCTC 
CAATATGTAT TTAATCTGGT ACCTAGAGGT CAGTACACCT CTGGAAAGGG ATCCAGTGCA 
GTTGGGTTAA CCGCATACGT GATGAAGGAC CCAGAAACGA GGCAGTTGGT ACTGCAGACA 
GGAGCATTGG TTCTTAGTGA CAATGGTATT TGCTGCATTG ATGAATTTGA CAAAATGAAT 
GAGAGCACTA GATCTGTGCT ACATGAAGTA ATGGAACAGC AAACCCTCTC CATTGCTAAG 
GCTGGAATTA TCTGTCAGCT GAATGCACGT ACATCTGTTT TGGCTGCTGC AAACCCGGTA 
GAGTCACAAT GGAACCCCAA GAAAACCACT ATAGAAAATA TTCAGCTCCC ACATACACTC 
TTATCAAGGT TTGATCTAAT ATTTTTGATG CTGGATCCAC AAGATGAAGC ATATGATCGC 
CGCTTAGCCC ACCACCTTGT GGCTTTGTAC TACCAGAGTG AGGAGCAGAT GAAAGAAGAG 
CACTTGGACA TGGCAGTATT AAAGGATTAT ATTGCATATG CTCGCACTTA TGTGAATCCA 
AGACTTAGTG AAGAAGCAAG CCAGGCTCTA ATAGAGGCCT ATGTCAGCAT GCGGAAAATC 
GGCAGTGGTC GTGGCATGGT TTCTGCCTAT CCCAGGCAGC TTGAGTCTTT AATACGCCGA 
GCAGAAGCTC ATGCGAAAGT ACGCTTTTCT AACAAGGTGG AAACAATTGA TGTAGAAGAG 
GCCAAACGTC TTCATCGTGA GGCTTTGAAA CAGTCTGCCA CTGATCCCAG AACTGGCATT 
GTTGATATAT CTATTCTTAC AACAGGAATG AGTGCAACTG CTCGAAAACG TAAAGAGGAA 
TTGGCTCAGG TTTTAAAGAA GCTCATCCAG TCCAAGGGCA AAACTCCAGC GTTAAAATAC 
CAGCAGCTCT TTGAGGATCT ACGTGGGCAG TCTGATGCAG CCATTACAAA AGACATGTTT 
GATGAAGCTT TGCATGCCCT TGCGGATGAC GACTACTTAA CAGTGACAGG AAAGACTGTA 
AGGTTGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001081448.1
CDS	63..2654
Translation	MSSPTSTPSRRRNKRGRGSNPPTPHGEEVQSPPSQRRRTEDSTSIGELLPMPTSPSGDVQSPSGQELLFSSPVPSRHSAHQSELDLSSPLTYGTPSSRVEGTPRSGIRGTPARQRPDLGSARKVKQVDLHSDQPAAEELVTSEQSLGQKLVIWGTDVNVATCKEKFQRFVQRFIDPSAKEEDNVGLDLNEPIYMQRLEEINVVGDPFLNIDCDHLRNFDQDLYRQLVCYPQEVIPTFDMAANEIFFERYPDSILEHQIQVRPYNALKTRNMRSLNPEDIDQLITISGMVIRTSQIIPEMQEAFFKCQVCAFTTRVEIDRGRIAEPSVCKHCNTTHSMALIHNRSMFSDKQMIKLQESPEDMPAGQTPHTTILYGHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVRNVKSVYKTHIDVIHYRKTDSKRLHGIDEDTEQKLFTEERVAMLKELAAKPDIYERLAAALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEVNILLCGDPGTSKSQLLQYVFNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPVESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQMKEEHLDMAVLKDYIAYARTYVNPRLSEEASQALIEAYVSMRKIGSGRGMVSAYPRQLESLIRRAEAHAKVRFSNKVETIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATARKRKEELAQVLKKLIQSKGKTPALKYQQLFEDLRGQSDAAITKDMFDEALHALADDDYLTVTGKTVRLL

Target ORF information:

RefSeq Version	NM_001087979.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis minichromosome maintenance complex component 4 L homeolog (mcm4.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001087979.1

ORF Insert Sequence:

ATGTCCTCTC CAACATCCAC ACCAAGCCGC AGGAGGAACA AACGTGGGCG AGGCAGTAAC 
CCTCCAACTC CTCATGGAGA GGAAGTGCAG TCTCCTCCGT CACAGAGACG ACGGACAGAA 
GATTCCACAT CAATTGGTGA ACTACTGCCC ATGCCAACCT CTCCTTCAGG TGACGTTCAG 
AGCCCCAGTG GACAAGAGCT CTTGTTTTCT AGCCCTGTAC CATCAAGACA TTCAGCCCAT 
CAAAGTGAGC TTGATCTTAG TTCTCCTCTG ACTTATGGCA CCCCTAGCTC AAGGGTTGAG 
GGTACCCCAA GAAGTGGTAT CCGAGGGACA CCTGCTAGAC AGAGACCTGA CTTGGGATCA 
GCACGGAAAG TTAAGCAAGT GGATTTACAT TCAGACCAGC CTGCTGCAGA GGAGCTAGTT 
ACGAGTGAGC AATCTTTGGG ACAGAAGTTG GTGATCTGGG GAACTGATGT CAATGTAGCC 
ACTTGCAAAG AAAAGTTTCA GCGTTTTGTT CAGCGTTTCA TTGATCCTTC AGCCAAAGAA 
GAGGATAATG TAGGACTGGA TCTGAATGAG CCTATATATA TGCAGCGACT TGAAGAGATA 
AATGTGGTAG GTGATCCATT TCTCAACATT GACTGTGACC ATCTCAGAAA CTTTGACCAG 
GATCTTTACA GGCAGCTAGT GTGCTACCCA CAGGAAGTCA TTCCAACATT TGATATGGCT 
GCTAATGAGA TTTTCTTTGA GCGGTATCCT GACTCCATTT TGGAGCACCA GATTCAAGTT 
CGACCCTATA ATGCATTGAA AACTAGGAAT ATGAGAAGCC TAAATCCTGA AGATATTGAT 
CAACTTATCA CAATTAGTGG CATGGTTATC AGAACTTCAC AAATCATCCC TGAGATGCAG 
GAAGCCTTTT TTAAATGTCA AGTGTGTGCC TTCACTACTC GGGTGGAGAT TGATCGTGGT 
CGGATTGCAG AGCCATCGGT CTGCAAACAC TGTAACACCA CACACAGCAT GGCTCTTATT 
CACAATCGCT CTATGTTTTC AGACAAGCAA ATGATTAAAC TGCAAGAGTC TCCAGAAGAT 
ATGCCTGCTG GTCAGACCCC TCATACAACT ATCCTCTATG GTCATAATGA CTTGGTGGAT 
AAGGTACAGC CTGGTGATAG AGTGAATGTC ACAGGTATTT ATAGAGCTGT TCCAATTCGA 
GTTAACCCTA GAGTTCGAAA TGTGAAGTCT GTTTACAAAA CTCACATCGA TGTAATTCAT 
TACCGAAAAA CTGATTCAAA GCGGTTGCAT GGTATTGATG AAGACACTGA ACAGAAGTTG 
TTCACTGAAG AAAGGGTTGC AATGTTAAAG GAACTGGCAG CAAAGCCAGA CATCTATGAG 
AGGCTTGCTG CAGCTCTTGC ACCAAGTATA TATGAGCATG AAGATATAAA AAAGGGAATT 
TTACTTCAGT TGTTTGGTGG GACACGGAAG GACTTCAGCC ATACCGGAAG GGGCAAATTC 
CGAGCTGAAG TAAACATACT TTTGTGTGGT GATCCAGGAA CGAGTAAATC TCAGCTTCTC 
CAATATGTAT TTAATCTGGT ACCTAGAGGT CAGTACACCT CTGGAAAGGG ATCCAGTGCA 
GTTGGGTTAA CCGCATACGT GATGAAGGAC CCAGAAACGA GGCAGTTGGT ACTGCAGACA 
GGAGCATTGG TTCTTAGTGA CAATGGTATT TGCTGCATTG ATGAATTTGA CAAAATGAAT 
GAGAGCACTA GATCTGTGCT ACATGAAGTA ATGGAACAGC AAACCCTCTC CATTGCTAAG 
GCTGGAATTA TCTGTCAGCT GAATGCACGT ACATCTGTTT TGGCTGCTGC AAACCCGGTA 
GAGTCACAAT GGAACCCCAA GAAAACCACT ATAGAAAATA TTCAGCTCCC ACATACACTC 
TTATCAAGGT TTGATCTAAT ATTTTTGATG CTGGATCCAC AAGATGAAGC ATATGATCGC 
CGCTTAGCCC ACCACCTTGT GGCTTTGTAC TACCAGAGTG AGGAGCAGAT GAAAGAAGAG 
CACTTGGACA TGGCAGTATT AAAGGATTAT ATTGCATATG CTCGCACTTA TGTGAATCCA 
AGACTTAGTG AAGAAGCAAG CCAGGCTCTA ATAGAGGCCT ATGTCAGCAT GCGGAAAATC 
GGCAGTGGTC GTGGCATGGT TTCTGCCTAT CCCAGGCAGC TTGAGTCTTT AATACGCCGA 
GCAGAAGCTC ATGCGAAAGT ACGCTTTTCT AACAAGGTGG AAACAATTGA TGTAGAAGAG 
GCCAAACGTC TTCATCGTGA GGCTTTGAAA CAGTCTGCCA CTGATCCCAG AACTGGCATT 
GTTGATATAT CTATTCTTAC AACAGGAATG AGTGCAACTG CTCGAAAACG TAAAGAGGAA 
TTGGCTCAGG TTTTAAAGAA GCTCATCCAG TCCAAGGGCA AAACTCCAGC GTTAAAATAC 
CAGCAGCTCT TTGAGGATCT ACGTGGGCAG TCTGATGCAG CCATTACAAA AGACATGTTT 
GATGAAGCTT TGCATGCCCT TGCGGATGAC GACTACTTAA CAGTGACAGG AAAGACTGTA 
AGGTTGCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The ATPase activity of MCM2-7 is dispensable for pre-RC assembly but is required for DNA unwinding.
The EMBO journal24(24)4334-44(2005 Dec)
Ying CY,Gautier J

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Distinct phosphoisoforms of the Xenopus Mcm4 protein regulate the function of the Mcm complex.
Molecular and cellular biology20(10)3667-76(2000 May)
Pereverzeva I,Whitmire E,Khan B,Cou? M

Evidence for different MCM subcomplexes with differential binding to chromatin in Xenopus.
Experimental cell research245(2)282-9(1998 Dec)
Cou? M,Amariglio F,Maiorano D,Bocquet S,M?chali M

Developmental regulation of MCM replication factors in Xenopus laevis.
Current biology : CB8(6)347-50(1998 Mar)
Sible JC,Erikson E,Hendrickson M,Maller JL,Gautier J