mcm3.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	mcm3.L
Entrez Gene ID	397821
Full Name	minichromosome maintenance complex component 3 L homeolog
Synonyms	mcm3,xmcm3
General protein information	Preferred Names minichromosome maintenance complex component 3 L homeolog Names maternal DNA replication licensing factor mcm3 P1 homolog XRLF subunit beta mMCM3 maternal Mcm3 protein maternal minichromosome maintenance protein 3 p100
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001087943.1	NP_001081412.1	maternal DNA replication licensing factor mcm3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

MCM interference during licensing of DNA replication in Xenopus egg extracts- Possible Role of A C-terminal region of MCM3.
Mimura S, Kubota Y, Takisawa H
Cell cycle (Georgetown, Tex.)1-53(2017 Dec)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells.
Madine MA, Khoo CY, Mills AD, Laskey RA
Nature375(6530)421-4(1995 Jun)

Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor.
Kubota Y, Mimura S, Nishimoto S, Takisawa H, Nojima H
Cell81(4)601-9(1995 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mcm3.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mcm3.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf00846	NM_001087943.1 Latest version!	Xenopus laevis minichromosome maintenance complex component 3 L homeolog (mcm3.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mcm3.L ( NM_001087943.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001081412.1 Xenopus laevis minichromosome maintenance complex component 3 L homeolog (mcm3.L), mRNA.

Price & Availability↑

CloneID	OAf00846
Clone ID Related Accession (Same CDS sequence)	NM_001087943.1
Accession Version	NM_001087943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Xenopus laevis(African clawed frog)
Product	maternal DNA replication licensing factor mcm3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC110950.1. On Jul 25, 2007 this sequence version replaced NM_001087944.1. ##Evidence-Data-START## Transcript exon combination :: BC110950.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00012419, SAMN02777034 [ECO:0000350] ##Evidence-Data-END##

ATGGATTACG GAGGTGGGTT TGAGGATCAT GAGCTGCGGG AGGCGCAGAG GGAATATTTA 
GATTTCCTAG ATGATGATCA AGATCAAGGC CTTTACCATG GGAAGGTGAG GGACATGATT 
GGCTCCAATG AGCACCGGCT GATCGTCAAC CTGAATGATG TGAGGAGGAA GAATGACAAA 
AGAGCCAACC TGATGCTGAA TGATGCGTTT GCAGAAACTA TTGCTTTTCA GAGGGCATTA 
AAAGATCTGG TGGCTTCCAT TGATGCAACA TATGCCAAGC AGTTTGAGGA GTTCAGTGTT 
GGATTCGAAG GCAGCTTTGG CAGCAAGCAT GTCTCTCCCC GTACCCTAAC AGCCAGTCTC 
CTGGGCTCTT TGGTTTGTGT GGAAGGGATC GTGACCAAAT GTTCCTTGGT TAGACCCAAA 
GTAATGCGCA GTGTCCATTA TTGTCCAGCC ACCAAAAAAA CCCTTGAAAG GAAATACTCT 
GACCTTACAT CCCTGGAAGC ATTTCCTTCT AGCTCCATTT ATCCTACAAA GGATGAGGAA 
AACAACCCAC TGGAAACAGA ATATGGACTG AGCACTTACA AGGACCACCA AACCTTGAGT 
ATCCAAGAAA TGCCAGAAAA GGCCCCAGCT GGGCAGCTTC CTCGTTCTGT TGACATTATT 
GCAGATGATG ATCTAGTAGA TAAATGTAAA CCAGGTGATA GAGTGCAGAT AGTAGGGATA 
TACCGTTGCC TTCCTTCCAA GCAAGGGGGC TTCACATCTG GAACTTTCAG GACCATATTA 
TTGGCTAATA ACATTAAGTT GATGAGTAAG GAGATTGCGC CAACATTTTC AGCTGATGAT 
GTAGCTAAAA TCAAGAAATT CTGCAAAGCT CATTCCAAAG ACATATTTGA ACATTTAAGC 
AAATCGTTGG CCCCCAGTAT TCATGGCCAT GAGTATATTA AGAAGGCCAT TTTGTGTATG 
CTTCTTGGGG GCAATGAGAA GGTTCTTGAG AATGGCACAC GTATAAGAGG AGATATCAAT 
GTTCTCCTAA TAGGAGATCC CTCAGTTGCC AAGTCTCAGC TATTGCGCTA TGTACTGCAC 
ACAGCTCCAC GAGCCATCCC TACTACTGGT AGAGGATCCT CTGGAGTAGG GTTAACGGCA 
GCTGTCACAA CGGACCAGGA AACTGGGGAG AGGCGCTTGG AAGCTGGGGC TATGGTGCTT 
GCAGACAGAG GAGTGGTGTG CATTGATGAA TTTGACAAAA TGTCTGATAT GGACAGGACT 
GCAATCCATG AAGTGATGGA GCAGGGCCGA GTGACAATTG CAAAAGCAGG GATCCAGGCA 
CGATTAAATG CCCGATGTAG CGTACTGGCA GCAGCAAATC CTGTTTATGG AAGGTATGAC 
CAATATAGAA CTCCAATGGA GAACATTGGG TTGCAGGATT CTCTGCTTTC AAGATTTGAC 
TTGCTTTTTA TTGTCCTGGA CAAAATGGAT GCAGACAATG ATCAAGAAAT TGCTGACCAT 
GTTCTGAGGA TGCACAGATA TAGGACTCCA GGGGAACAAG ATGGTTATGC ATTGCCTCTT 
GGATGCAGTG TTGAGATCTT TGCCACAGAT GATCCAAATG CCTCTGATGT TACAGATCAA 
GAGCTGCAAA TTTATGAGAA ACATGATAAT TTACTCCATG GGCCACGGAA AAACAAGTCA 
AAGATTGTGA GCATGCAGTT TATTCGGAAG TATATCCATG TTGCTAAACT GATAAAGCCC 
GTCCTGACTT CCGAAGCTGC TGACTACATA TCTCAGGAAT ATGCAAAGAT CAGGAACCAC 
GATCAGATAA ATAATGATAG TGCCAGGACA ATGCCAGTTA CTGCCAGAGC GCTGGAAACC 
ATGATCAGAT TGTCTACTGC TCACGCTAAG GTCAGAATGA GCAAAACCAT TGAGCGCCAG 
GATGCTGAAA CTGCACTTGA GCTGGTCCAG TTTGCCTATT TCAAGAAGGT CTTGGCAAAA 
GAAAAGAAAA AAACAGACAA GGATCTGCAT GATGAAAACT TAAGTCAAGA CACACTTAGC 
CAAGAGAGTG TTCGGAAAAG TTCTAGACGT GCAGGAAAAA TTGCTGACTC TCAAGATGAT 
TCAATGGACC CATACTCTTT TAGTGAGCAA GATAGCAGTC TAAATGAAAA CCTTTCTCAG 
AGTTTAAGAC CTCAACGGAA AAAAGCAGAA AGTCAAGATG GCAAGCGGTC CTTGAGTCAA 
AACAGGACCA AAGAGTTTAA AGCTGCCCTC CTGAAAGCCT TTAAGAGCAG TCGGTCACAG 
TCTGTTGCGG TCAGTCAGCT GCTGGAGCTC ATCAACAAGG GGAATCCAGA GCCTTTTGAA 
CGGAGTGAAG TAAAGGAGGC CTTGGACAAT ATGCAGAATG ACAATCAGGT CATGGTGTCA 
GAAGATGTGG TTTTCCTAAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001081412.1
CDS	4..2427
Translation	MDYGGGFEDHELREAQREYLDFLDDDQDQGLYHGKVRDMIGSNEHRLIVNLNDVRRKNDKRANLMLNDAFAETIAFQRALKDLVASIDATYAKQFEEFSVGFEGSFGSKHVSPRTLTASLLGSLVCVEGIVTKCSLVRPKVMRSVHYCPATKKTLERKYSDLTSLEAFPSSSIYPTKDEENNPLETEYGLSTYKDHQTLSIQEMPEKAPAGQLPRSVDIIADDDLVDKCKPGDRVQIVGIYRCLPSKQGGFTSGTFRTILLANNIKLMSKEIAPTFSADDVAKIKKFCKAHSKDIFEHLSKSLAPSIHGHEYIKKAILCMLLGGNEKVLENGTRIRGDINVLLIGDPSVAKSQLLRYVLHTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIQARLNARCSVLAAANPVYGRYDQYRTPMENIGLQDSLLSRFDLLFIVLDKMDADNDQEIADHVLRMHRYRTPGEQDGYALPLGCSVEIFATDDPNASDVTDQELQIYEKHDNLLHGPRKNKSKIVSMQFIRKYIHVAKLIKPVLTSEAADYISQEYAKIRNHDQINNDSARTMPVTARALETMIRLSTAHAKVRMSKTIERQDAETALELVQFAYFKKVLAKEKKKTDKDLHDENLSQDTLSQESVRKSSRRAGKIADSQDDSMDPYSFSEQDSSLNENLSQSLRPQRKKAESQDGKRSLSQNRTKEFKAALLKAFKSSRSQSVAVSQLLELINKGNPEPFERSEVKEALDNMQNDNQVMVSEDVVFLI

Target ORF information:

RefSeq Version	NM_001087943.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis minichromosome maintenance complex component 3 L homeolog (mcm3.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001087943.1

ORF Insert Sequence:

ATGGATTACG GAGGTGGGTT TGAGGATCAT GAGCTGCGGG AGGCGCAGAG GGAATATTTA 
GATTTCCTAG ATGATGATCA AGATCAAGGC CTTTACCATG GGAAGGTGAG GGACATGATT 
GGCTCCAATG AGCACCGGCT GATCGTCAAC CTGAATGATG TGAGGAGGAA GAATGACAAA 
AGAGCCAACC TGATGCTGAA TGATGCGTTT GCAGAAACTA TTGCTTTTCA GAGGGCATTA 
AAAGATCTGG TGGCTTCCAT TGATGCAACA TATGCCAAGC AGTTTGAGGA GTTCAGTGTT 
GGATTCGAAG GCAGCTTTGG CAGCAAGCAT GTCTCTCCCC GTACCCTAAC AGCCAGTCTC 
CTGGGCTCTT TGGTTTGTGT GGAAGGGATC GTGACCAAAT GTTCCTTGGT TAGACCCAAA 
GTAATGCGCA GTGTCCATTA TTGTCCAGCC ACCAAAAAAA CCCTTGAAAG GAAATACTCT 
GACCTTACAT CCCTGGAAGC ATTTCCTTCT AGCTCCATTT ATCCTACAAA GGATGAGGAA 
AACAACCCAC TGGAAACAGA ATATGGACTG AGCACTTACA AGGACCACCA AACCTTGAGT 
ATCCAAGAAA TGCCAGAAAA GGCCCCAGCT GGGCAGCTTC CTCGTTCTGT TGACATTATT 
GCAGATGATG ATCTAGTAGA TAAATGTAAA CCAGGTGATA GAGTGCAGAT AGTAGGGATA 
TACCGTTGCC TTCCTTCCAA GCAAGGGGGC TTCACATCTG GAACTTTCAG GACCATATTA 
TTGGCTAATA ACATTAAGTT GATGAGTAAG GAGATTGCGC CAACATTTTC AGCTGATGAT 
GTAGCTAAAA TCAAGAAATT CTGCAAAGCT CATTCCAAAG ACATATTTGA ACATTTAAGC 
AAATCGTTGG CCCCCAGTAT TCATGGCCAT GAGTATATTA AGAAGGCCAT TTTGTGTATG 
CTTCTTGGGG GCAATGAGAA GGTTCTTGAG AATGGCACAC GTATAAGAGG AGATATCAAT 
GTTCTCCTAA TAGGAGATCC CTCAGTTGCC AAGTCTCAGC TATTGCGCTA TGTACTGCAC 
ACAGCTCCAC GAGCCATCCC TACTACTGGT AGAGGATCCT CTGGAGTAGG GTTAACGGCA 
GCTGTCACAA CGGACCAGGA AACTGGGGAG AGGCGCTTGG AAGCTGGGGC TATGGTGCTT 
GCAGACAGAG GAGTGGTGTG CATTGATGAA TTTGACAAAA TGTCTGATAT GGACAGGACT 
GCAATCCATG AAGTGATGGA GCAGGGCCGA GTGACAATTG CAAAAGCAGG GATCCAGGCA 
CGATTAAATG CCCGATGTAG CGTACTGGCA GCAGCAAATC CTGTTTATGG AAGGTATGAC 
CAATATAGAA CTCCAATGGA GAACATTGGG TTGCAGGATT CTCTGCTTTC AAGATTTGAC 
TTGCTTTTTA TTGTCCTGGA CAAAATGGAT GCAGACAATG ATCAAGAAAT TGCTGACCAT 
GTTCTGAGGA TGCACAGATA TAGGACTCCA GGGGAACAAG ATGGTTATGC ATTGCCTCTT 
GGATGCAGTG TTGAGATCTT TGCCACAGAT GATCCAAATG CCTCTGATGT TACAGATCAA 
GAGCTGCAAA TTTATGAGAA ACATGATAAT TTACTCCATG GGCCACGGAA AAACAAGTCA 
AAGATTGTGA GCATGCAGTT TATTCGGAAG TATATCCATG TTGCTAAACT GATAAAGCCC 
GTCCTGACTT CCGAAGCTGC TGACTACATA TCTCAGGAAT ATGCAAAGAT CAGGAACCAC 
GATCAGATAA ATAATGATAG TGCCAGGACA ATGCCAGTTA CTGCCAGAGC GCTGGAAACC 
ATGATCAGAT TGTCTACTGC TCACGCTAAG GTCAGAATGA GCAAAACCAT TGAGCGCCAG 
GATGCTGAAA CTGCACTTGA GCTGGTCCAG TTTGCCTATT TCAAGAAGGT CTTGGCAAAA 
GAAAAGAAAA AAACAGACAA GGATCTGCAT GATGAAAACT TAAGTCAAGA CACACTTAGC 
CAAGAGAGTG TTCGGAAAAG TTCTAGACGT GCAGGAAAAA TTGCTGACTC TCAAGATGAT 
TCAATGGACC CATACTCTTT TAGTGAGCAA GATAGCAGTC TAAATGAAAA CCTTTCTCAG 
AGTTTAAGAC CTCAACGGAA AAAAGCAGAA AGTCAAGATG GCAAGCGGTC CTTGAGTCAA 
AACAGGACCA AAGAGTTTAA AGCTGCCCTC CTGAAAGCCT TTAAGAGCAG TCGGTCACAG 
TCTGTTGCGG TCAGTCAGCT GCTGGAGCTC ATCAACAAGG GGAATCCAGA GCCTTTTGAA 
CGGAGTGAAG TAAAGGAGGC CTTGGACAAT ATGCAGAATG ACAATCAGGT CATGGTGTCA 
GAAGATGTGG TTTTCCTAAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MCM interference during licensing of DNA replication in Xenopus egg extracts- Possible Role of A C-terminal region of MCM3.
Cell cycle (Georgetown, Tex.)1-53(2017 Dec)
Mimura S,Kubota Y,Takisawa H

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells.
Nature375(6530)421-4(1995 Jun)
Madine MA,Khoo CY,Mills AD,Laskey RA

Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor.
Cell81(4)601-9(1995 May)
Kubota Y,Mimura S,Nishimoto S,Takisawa H,Nojima H