GS cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	GS
Entrez Gene ID	100266191
Full Name	glutamine synthetase
Synonyms	GS1,GS1-1
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001281246.1	NP_001268175.1	glutamine synthetase cytosolic isozyme 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The high polyphenol content of grapevine cultivar tannat berries is conferred primarily by genes that are not shared with the reference genome.
Da Silva C, Zamperin G, Ferrarini A, Minio A, Dal Molin A, Venturini L, Buson G, Tononi P, Avanzato C, Zago E, Boido E, Dellacassa E, Gaggero C, Pezzotti M, Carrau F, Delledonne M
The Plant cell25(12)4777-88(2013 Dec)

Characterization of Vitis vinifera L. glutamine synthetase and molecular cloning of cDNAs for the cytosolic enzyme.
Loulakakis KA, Roubelakis-Angelakis KA
Plant molecular biology31(5)983-92(1996 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa00002	NM_001281246.1 Latest version!	Vitis vinifera glutamine synthetase (GS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GS ( NM_001281246.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> NP_001268175.1 Vitis vinifera glutamine synthetase (GS), mRNA.

Price & Availability↑

CloneID	OVa00002
Clone ID Related Accession (Same CDS sequence)	NM_001281246.1
Accession Version	NM_001281246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Vitis vinifera(wine grape)
Product	glutamine synthetase cytosolic isozyme 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X94320.1. On Jul 24, 2013 this sequence version replaced XM_002283135.2. ##Evidence-Data-START## Transcript exon combination :: X94320.1, FQ394632.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01057004, SAMN01057009 [ECO:0000348] ##Evidence-Data-END##

ATGGCACTGC TCTCCGATCT CATCAACTTG AACCTCTCTG AGACCACTGA GAAGGTGATT 
GTAGAGTACA TATGGGTTGG TGGATCTGGT ATGGACCTTA GAAGCAAAGC CAGGACCCTT 
TCTGGACCAG TTAGCGATCC TGCAAAGCTT CCCAAATGGA ACTATGATGG TTCTAGCACT 
GGCCAAGCTC CGGGGGAAGA CAGTGAAGTT ATTCTATATC CTCAAGCAAT TTTCAAGGAC 
CCATTCAGGA GAGGCAACAA CATTCTTGTG ATGTGTGATA CTTATACCCC TGCCGGAGAG 
CCAATTCCGA CCAATAAGAG GTGTAATGCT GCAAAGATTT TCAGCCACCC TGATGTTGCA 
GCTGAAGTGC CTTGGTATGG TATTGAGCAG GAGTATACTT TGTTGCAGAA AGAGGTAAAA 
TGGCCAATTG GCTGGCCTGT GGGCGGTTTT CCTGGACCTC AGGGACCATA CTATTGTGGT 
ATTGGTGCTG ACAAAGCTTG GGGCCGTGAC ATTGTTGATG CACATTACAA AGCGTGCCTT 
TATGCTGGCA TCAACATCAG TGGAATCAAT GGAGAAGTGA TGCCAGGCCA GTGGGAATAC 
CAAGTTGGCC CTTCTGTTGG CATCTCTGCA GGAGATGAAT TATGGGTGTC TCGTTATATT 
CTTGAGAGGA TCACAGAGAT TGCTGGGGTG GTGCTTTCTT TTGACCCGAA ACCCATCCAG 
GGTGATTGGA ATGGAGCGGG TGCGCACACA AATTACAGCA CCAAGTCGAT GAGAAATGAT 
GGAGGGTTTG AAGTCATCAA AAAAGCCATT GAAAAGCTTG GGCTGAGGCA CAAGGAACAC 
ATTGCAGCTT ATGGAGAAGG CAATGAACGC CGTCTCACTG GACGACATGA AACTGCTGAC 
ATCAACACAT TCTTATGGGG AGTGGCAAAC CGAGGAGCCT CCATTAGAGT TGGAAGAGAC 
ACAGAGAAAG CTGGGAAAGG ATATTTTGAG GACAGAAGGC CTGCTTCAAA CATGGATCCT 
TATGTGGTCA CCTCCATGAT TGCAGAAACT ACCATCCTCT GGAAGCCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268175.1
CDS	88..1158
Translation	MALLSDLINLNLSETTEKVIVEYIWVGGSGMDLRSKARTLSGPVSDPAKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDTYTPAGEPIPTNKRCNAAKIFSHPDVAAEVPWYGIEQEYTLLQKEVKWPIGWPVGGFPGPQGPYYCGIGADKAWGRDIVDAHYKACLYAGINISGINGEVMPGQWEYQVGPSVGISAGDELWVSRYILERITEIAGVVLSFDPKPIQGDWNGAGAHTNYSTKSMRNDGGFEVIKKAIEKLGLRHKEHIAAYGEGNERRLTGRHETADINTFLWGVANRGASIRVGRDTEKAGKGYFEDRRPASNMDPYVVTSMIAETTILWKP

Target ORF information:

RefSeq Version	NM_001281246.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera glutamine synthetase (GS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281246.1

ORF Insert Sequence:

ATGGCACTGC TCTCCGATCT CATCAACTTG AACCTCTCTG AGACCACTGA GAAGGTGATT 
GTAGAGTACA TATGGGTTGG TGGATCTGGT ATGGACCTTA GAAGCAAAGC CAGGACCCTT 
TCTGGACCAG TTAGCGATCC TGCAAAGCTT CCCAAATGGA ACTATGATGG TTCTAGCACT 
GGCCAAGCTC CGGGGGAAGA CAGTGAAGTT ATTCTATATC CTCAAGCAAT TTTCAAGGAC 
CCATTCAGGA GAGGCAACAA CATTCTTGTG ATGTGTGATA CTTATACCCC TGCCGGAGAG 
CCAATTCCGA CCAATAAGAG GTGTAATGCT GCAAAGATTT TCAGCCACCC TGATGTTGCA 
GCTGAAGTGC CTTGGTATGG TATTGAGCAG GAGTATACTT TGTTGCAGAA AGAGGTAAAA 
TGGCCAATTG GCTGGCCTGT GGGCGGTTTT CCTGGACCTC AGGGACCATA CTATTGTGGT 
ATTGGTGCTG ACAAAGCTTG GGGCCGTGAC ATTGTTGATG CACATTACAA AGCGTGCCTT 
TATGCTGGCA TCAACATCAG TGGAATCAAT GGAGAAGTGA TGCCAGGCCA GTGGGAATAC 
CAAGTTGGCC CTTCTGTTGG CATCTCTGCA GGAGATGAAT TATGGGTGTC TCGTTATATT 
CTTGAGAGGA TCACAGAGAT TGCTGGGGTG GTGCTTTCTT TTGACCCGAA ACCCATCCAG 
GGTGATTGGA ATGGAGCGGG TGCGCACACA AATTACAGCA CCAAGTCGAT GAGAAATGAT 
GGAGGGTTTG AAGTCATCAA AAAAGCCATT GAAAAGCTTG GGCTGAGGCA CAAGGAACAC 
ATTGCAGCTT ATGGAGAAGG CAATGAACGC CGTCTCACTG GACGACATGA AACTGCTGAC 
ATCAACACAT TCTTATGGGG AGTGGCAAAC CGAGGAGCCT CCATTAGAGT TGGAAGAGAC 
ACAGAGAAAG CTGGGAAAGG ATATTTTGAG GACAGAAGGC CTGCTTCAAA CATGGATCCT 
TATGTGGTCA CCTCCATGAT TGCAGAAACT ACCATCCTCT GGAAGCCATA A