TNF cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNF
Entrez Gene ID	101318178
Full Name	tumor necrosis factor
Synonyms	Cachectin,TNF-a,TNF-alpha,TNFA,TNFSF2
Gene Type	protein-coding
Organism	Tursiops truncatus(common bottlenose dolphin)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001280615.1	NP_001267544.1	tumor necrosis factor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Quantitation of leukocyte gene expression in cetaceans.
Sitt T, Bowen L, Blanchard MT, Smith BR, Gershwin LJ, Byrne BA, Stott JL
Developmental and comparative immunology32(11)1253-9(2008)

Molecular cloning and functional characterization of bottlenose dolphin (Tursiops truncatus) tumor necrosis factor alpha.
Shoji Y, Inoue Y, Sugisawa H, Itou T, Endo T, Sakai T
Veterinary immunology and immunopathology82(3-4)183-92(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTu29696	NM_001280615.1 Latest version!	Tursiops truncatus tumor necrosis factor (TNF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNF ( NM_001280615.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> NP_001267544.1 Tursiops truncatus tumor necrosis factor (TNF), mRNA.

Price & Availability↑

CloneID	OTu29696
Clone ID Related Accession (Same CDS sequence)	NM_001280615.1
Accession Version	NM_001280615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	tumor necrosis factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB049358.1. On Jul 23, 2013 this sequence version replaced XM_004311221.1. ##Evidence-Data-START## Transcript exon combination :: AB049358.1, DV799582.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCACTG AAAGCATGAT CCGGGATGTG GAGCTGGCAG AGGAGGCGCT CTCCAAGACG 
GCAGGGGGCT CCCAGGGTTC CGGAAGGTGC TTGTGTCTCA GCCTCTTCTC CTTCTTCCTG 
GTCGCAGGAG GCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGTTCC CAACTGGCTA CTCCATCATC AGCCCTCTGG CTCAGACACT CAGATCATCT 
TCTAAAACCT CAAGTAACAA GCCCGTAGCC CATGTTGTAG CCAACCTCAG TACTCAGGGG 
CAGCTCCGGT GGCTGAACAC ATATGCCAAC ACCCTCCTGG CCAACAGCGT GAAGCTGGAA 
GACAACCAGC TGGTGGTGCC GACGGATGGG CTGTACCTCA TCTACTCCCA GGTCCTCTTC 
AGGGGCCAAG GCTGCCCTTC CACCCACTTG TTCCTCACCC ACACCATCAG CCGCATCGCT 
GTCTCCTACC CGTCCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCTTG CCAGAGGGAG 
ACGCCAGAGG GGGCTGAGGC CAAGCCCTGG TACGAGCCCA TCTACCAGGG AGGGGTCTTC 
CAGCTGGAGA AGGGTGATCG ACTCAGTGCT GAGATCAACC TGCCCGACTA TCTGGACTTT 
GCTAAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001267544.1
CDS	198..899
Translation	MSTESMIRDVELAEEALSKTAGGSQGSGRCLCLSLFSFFLVAGGTTLFCLLHFGVIGPQREEFPTGYSIISPLAQTLRSSSKTSSNKPVAHVVANLSTQGQLRWLNTYANTLLANSVKLEDNQLVVPTDGLYLIYSQVLFRGQGCPSTHLFLTHTISRIAVSYPSKVNLLSAIKSPCQRETPEGAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPDYLDFAKSGQVYFGIIAL

Target ORF information:

RefSeq Version	NM_001280615.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001280615.1

ORF Insert Sequence:

ATGAGCACTG AAAGCATGAT CCGGGATGTG GAGCTGGCAG AGGAGGCGCT CTCCAAGACG 
GCAGGGGGCT CCCAGGGTTC CGGAAGGTGC TTGTGTCTCA GCCTCTTCTC CTTCTTCCTG 
GTCGCAGGAG GCACCACGCT CTTCTGCCTG CTGCACTTCG GGGTAATCGG CCCCCAGAGG 
GAAGAGTTCC CAACTGGCTA CTCCATCATC AGCCCTCTGG CTCAGACACT CAGATCATCT 
TCTAAAACCT CAAGTAACAA GCCCGTAGCC CATGTTGTAG CCAACCTCAG TACTCAGGGG 
CAGCTCCGGT GGCTGAACAC ATATGCCAAC ACCCTCCTGG CCAACAGCGT GAAGCTGGAA 
GACAACCAGC TGGTGGTGCC GACGGATGGG CTGTACCTCA TCTACTCCCA GGTCCTCTTC 
AGGGGCCAAG GCTGCCCTTC CACCCACTTG TTCCTCACCC ACACCATCAG CCGCATCGCT 
GTCTCCTACC CGTCCAAGGT CAACCTCCTC TCTGCCATCA AGAGCCCTTG CCAGAGGGAG 
ACGCCAGAGG GGGCTGAGGC CAAGCCCTGG TACGAGCCCA TCTACCAGGG AGGGGTCTTC 
CAGCTGGAGA AGGGTGATCG ACTCAGTGCT GAGATCAACC TGCCCGACTA TCTGGACTTT 
GCTAAGTCTG GGCAGGTCTA CTTTGGGATC ATTGCCCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitation of leukocyte gene expression in cetaceans.
Developmental and comparative immunology32(11)1253-9(2008)
Sitt T,Bowen L,Blanchard MT,Smith BR,Gershwin LJ,Byrne BA,Stott JL

Molecular cloning and functional characterization of bottlenose dolphin (Tursiops truncatus) tumor necrosis factor alpha.
Veterinary immunology and immunopathology82(3-4)183-92(2001 Oct)
Shoji Y,Inoue Y,Sugisawa H,Itou T,Endo T,Sakai T