TbgDal_VI2130 cDNA ORF clone, Trypanosoma brucei gambiense DAL972

Gene
Clones
gRNAs

Summary

Gene Symbol	TbgDal_VI2130
Entrez Gene ID	23861854
Full Name	ubiquitin-protein ligase, putative
Gene Type	protein-coding
Organism	Trypanosoma brucei gambiense DAL972

mRNA and Protein(s)

mRNA	Protein	Name
XM_011775718.1	XP_011774020.1	ubiquitin-protein ligase, putative

Pathways from BioSystems
Gene Ontology

The following TbgDal_VI2130 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TbgDal_VI2130 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTr201940	XM_011775718.1 Latest version!	Trypanosoma brucei gambiense DAL972 ubiquitin-protein ligase, putative partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TbgDal_VI2130 ( XM_011775718.1 ) cDNA ORF clone, Trypanosoma brucei gambiense DAL972 -> XP_011774020.1 Trypanosoma brucei gambiense DAL972 ubiquitin-protein ligase, putative partial mRNA.

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CloneID	OTr201940
Clone ID Related Accession (Same CDS sequence)	XM_011775718.1
Accession Version	XM_011775718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-29
Organism	Trypanosoma brucei gambiense DAL972
Product	ubiquitin-protein ligase, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026739). COMPLETENESS: incomplete on both ends.

ATGCATGGAA ATTTTGTATT TACCGGCAAA AGCCGGCCCA ACCGGCTCAC CCTCTCTAAC 
GATCGTCAAC CCTCAAAAAA CGTAATTTTG AACAATGCTC GCGCCCAGCG TGAGCAGCGG 
CAGAAAGAAA AGAGGGAGTG TCAGGCGGCG ACGTGCATCC AGAGAAACAC ACGAGGGTTA 
TTGGCACGCA TGGCTCTTGC TGAACTGGCG CGTGCTGCAC TAAAGGAAAT TAAAGAGACA 
CAGGATGCAG CTGCTGATGG TGGTACCTCT TCCGAAGCGG ATGAACCGTT AGAACGCCTT 
TGTTGGCTGT ACGAGTTTGT TCGTCGTGGA ATAGTTGGTC TGTTGGTTTG TAGCGTACAG 
TTACGCGAAT GCTCACAAAT TTTATTGAAT CGGTTGAGTG CTGTTTTGCG TGGTGGTGCC 
TTGGAGGAGC AACTGTTAGG TTTCCCCCAC GCAATTATGC TGTTAATGAA GTTGACACTG 
GAAGCATACT TAACACCTTC CTGTGATGAG GAAGCCGTAG TAGAACGAAG TAGGGAGCAT 
TTGCAGCCAC TAGCATCACT GTTTGCTGCT GCGAAGAAAC TGGGTAACGG AGCCGACATA 
CTTATCTGTG CACTTGATCT TGTCATTCGA CACAATGCAA CTCCCGATGA GGAAATTATT 
TCTGCACTGT ATGGTTTGTA TGCTACTCTG TCACCCGCTG CAGCTGCGAG TAGCGTGCAA 
TCTACTGCTA GTCGTACGTT GCTAGCTTAC GCCGTAGGCT CGACTTCCAA TGTTTCTTAT 
TATCTTTCCC CTACAACGTG TGCGCTGAGT GTTCTGTTAG GCACCGTGCG CAGGGATTGT 
TACCCACGGG TGAGTGAGGA AACGAATGCG ATATTCTTGG AGGCCATTGT GGACGTTACC 
CTGAAAATTT TAGAATCAAC GGCGAGCTCG CCGCCGTTGC AGATTCTTGG GAAATTGGTG 
AGAGTCCTAC CGTACTTCCA CCACGGTGCT GATGAAGATG CACAGAAGCA AGTTTTACGG 
CGGAAATGGC TCATTTGTCT TTCACGCGCG ACTCTCCTAT GCAGCTCCGA GGATGAACTT 
ATTCGAGAGA CGTTATCAGA TTTTCACCAT TCGCACACCA CCGCGCATCA GGAACATGCG 
GCCCACAAAC ACCTGTCAAC ATACCTATTT AGTACGGGGT ATGGCCTCCG TTTGCTTGAG 
GAGGGTCTGC GAACGAGTCT CTCGGCACAT AGCACGGATT CTTTCTCCGA CGTTAACCTT 
CTGTGTTGCG TGTTTATTTG GCCTCTCTAC AAATTTGCTA CTGCCTCTGC GCGCCAACGA 
CTTGAGGCAA CCACCATCAT TTCAAAGTTT GTTTATTCTT CAGGCCTTTT GCGGTGTCTG 
TGGGATGTGT ACCGAAATTG TCACAGCAGC ACCTTCGAAA GCACAGCTGC AATGAGCGCA 
GCGGAGCTGT TGGAGTGCGT GGCCACTGGG AAGCTGCTTG ACCTTCCTTC ACCCGTTGCC 
CCACCCACAG AGCTGCTCAG CAAGTTGTCG TTGCCTCGTT CCACGCACGG CCTGTTTTAC 
GATCCATACC CCGCTGTGTC GGTAACACTT TTTTGTCTGA TGAGTTACTT TGTTGACGCA 
ACAGGTTTCT TGGAAGGACT TGATCGCTGC GCGGTGTTTG ATCGTGCTGA CACTCTGAAG 
CTTATTTTTG CACTCAAGGG GATTTTGTAT CAGTCGTTCT TCCACGGTGT GTTGCCGTAC 
AGCAAATGTG AAGTGGTGGT GCAGGGGGCC CTCACATTGT TTTTAAAGCT GCACGTCGTA 
GGTGAGGCTC AATCGTTTGT TCCGCACCCG TCGGTTTGGA TAATTTGCCA CGATCACCTA 
CTTCTAAAGA CGTTTAAAAG TATTGAGTGT GGCACATGGT CCGCTATTGT GTCAAATGAA 
GATGATGAGG ATTGTACGGA GGATGACAAT GAAGAGCGGG AAGATGGACA TTGGGCACCA 
AGTTTGCGTG AAGGGAACCA TGAGTCGTAC GTTTCCCTTC CATGGTGGAA CGGGAGTCTT 
TCGTGGCCAC AGGAGGAACG TTGCATTCAT GTTTTGCAGC GTATCCCCTT TACCGTTCCA 
TTCGGAGCAC GTGTCACCTT GTTAACATCC TTTCTCTCTA GCCACGTGGA ACGCAGCCGT 
CCCACCACGT GGGGACACTT TGTGGTGCGT CGCGGTTGCA CTTTTGCCGA CGCTTTTGAC 
CGCTTTGCTG ATAACCCCGG TAGTAGTGAT ATGTATTATG TCCGTTTCCG TGCGGCAAAT 
GATTTGGTGG AAGAGGGTTA CGGCGACGGC GTCTACCGCG AATTTCTACT GTCCCTCTGC 
AAGGAGGGTT TTGCGGCGGA GCATGGGCTC TTTTGCTTGA CGGATGCTGG ATACGTTTAT 
CCAAATCCTT TCAGCTGGGA GGTGACTGGT GATCGGGATC ATCTCAAGCG TATCAAGTTT 
CTTGGTGCTA TGGTAGGCCG AGGATTGCGT GATGGTGTTT TGCAAGATAT TCCATTTGCT 
CTACATTTCC GTAATGCTAT TCTGGGGCGC AGCAACTCCA TCAATAACCT AAGGAGCTTT 
GATAGTCAAC TTTACCGCCA CCTTGTTTCT CTTATGAGTT TGAGCGAGGA AGAAATTGAA 
AACCTTGAGC TCAACTTCAC GTACACCGTG GAGGCCCTTG ATCGGGTGCA CGAAGTGGAG 
CTTCTACACG GTGGTCGCAA CATTGCTGTC ACTCGCCGCA ACTGTTTGAA TTATATTCAC 
TTGATTGCAG ACTTCAAACT TAATCGTGAA ACAGCCAAGC AAACACGGGC ATTTCGGAGT 
GGTCTGGAAA GTATCGTCCA CCGAAGTTGG TTACGACTCT TTGATAGTAA TGAGGTCATG 
AAGTTGTTCG GTGGTGATGT AGAGTGTAAC ATTGATGTGG AAGATTGGAA ACAGCACACT 
CAATACCACG TTCCTGACGA CGCCACTAGC AAACCCGTAC AAGTGTTCTG GGAGGTAGTG 
CAGTCTCTGC CGTTGGAACA GCAGAGGAAG CTTCTAAAGT TTTCCACTTC AATGAACCGC 
CCTCCGCTGC TTGGTTTCAA ATTTCTCAAC CCACCTTTCA AAATTCATGT CCTGTGGAAT 
GAGGCAGAAG AGCGATTGCC ATCTGCATCA ACGTGTTTCT GCACTCTAAA GCTTCCACCG 
TACCAGACGT TCGGGGTAGC GCAGCAAAAG ATAACAGCTG CAATTGAAGA GACGGATGAC 
TTCGGTCTCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011774020.1
CDS	1..3255
Translation	MHGNFVFTGKSRPNRLTLSNDRQPSKNVILNNARAQREQRQKEKRECQAATCIQRNTRGLLARMALAELARAALKEIKETQDAAADGGTSSEADEPLERLCWLYEFVRRGIVGLLVCSVQLRECSQILLNRLSAVLRGGALEEQLLGFPHAIMLLMKLTLEAYLTPSCDEEAVVERSREHLQPLASLFAAAKKLGNGADILICALDLVIRHNATPDEEIISALYGLYATLSPAAAASSVQSTASRTLLAYAVGSTSNVSYYLSPTTCALSVLLGTVRRDCYPRVSEETNAIFLEAIVDVTLKILESTASSPPLQILGKLVRVLPYFHHGADEDAQKQVLRRKWLICLSRATLLCSSEDELIRETLSDFHHSHTTAHQEHAAHKHLSTYLFSTGYGLRLLEEGLRTSLSAHSTDSFSDVNLLCCVFIWPLYKFATASARQRLEATTIISKFVYSSGLLRCLWDVYRNCHSSTFESTAAMSAAELLECVATGKLLDLPSPVAPPTELLSKLSLPRSTHGLFYDPYPAVSVTLFCLMSYFVDATGFLEGLDRCAVFDRADTLKLIFALKGILYQSFFHGVLPYSKCEVVVQGALTLFLKLHVVGEAQSFVPHPSVWIICHDHLLLKTFKSIECGTWSAIVSNEDDEDCTEDDNEEREDGHWAPSLREGNHESYVSLPWWNGSLSWPQEERCIHVLQRIPFTVPFGARVTLLTSFLSSHVERSRPTTWGHFVVRRGCTFADAFDRFADNPGSSDMYYVRFRAANDLVEEGYGDGVYREFLLSLCKEGFAAEHGLFCLTDAGYVYPNPFSWEVTGDRDHLKRIKFLGAMVGRGLRDGVLQDIPFALHFRNAILGRSNSINNLRSFDSQLYRHLVSLMSLSEEEIENLELNFTYTVEALDRVHEVELLHGGRNIAVTRRNCLNYIHLIADFKLNRETAKQTRAFRSGLESIVHRSWLRLFDSNEVMKLFGGDVECNIDVEDWKQHTQYHVPDDATSKPVQVFWEVVQSLPLEQQRKLLKFSTSMNRPPLLGFKFLNPPFKIHVLWNEAEERLPSASTCFCTLKLPPYQTFGVAQQKITAAIEETDDFGLS

Target ORF information:

RefSeq Version	XM_011775718.1
Organism	Trypanosoma brucei gambiense DAL972
Definition	Trypanosoma brucei gambiense DAL972 ubiquitin-protein ligase, putative partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011775718.1

ORF Insert Sequence:

ATGCATGGAA ATTTTGTATT TACCGGCAAA AGCCGGCCCA ACCGGCTCAC CCTCTCTAAC 
GATCGTCAAC CCTCAAAAAA CGTAATTTTG AACAATGCTC GCGCCCAGCG TGAGCAGCGG 
CAGAAAGAAA AGAGGGAGTG TCAGGCGGCG ACGTGCATCC AGAGAAACAC ACGAGGGTTA 
TTGGCACGCA TGGCTCTTGC TGAACTGGCG CGTGCTGCAC TAAAGGAAAT TAAAGAGACA 
CAGGATGCAG CTGCTGATGG TGGTACCTCT TCCGAAGCGG ATGAACCGTT AGAACGCCTT 
TGTTGGCTGT ACGAGTTTGT TCGTCGTGGA ATAGTTGGTC TGTTGGTTTG TAGCGTACAG 
TTACGCGAAT GCTCACAAAT TTTATTGAAT CGGTTGAGTG CTGTTTTGCG TGGTGGTGCC 
TTGGAGGAGC AACTGTTAGG TTTCCCCCAC GCAATTATGC TGTTAATGAA GTTGACACTG 
GAAGCATACT TAACACCTTC CTGTGATGAG GAAGCCGTAG TAGAACGAAG TAGGGAGCAT 
TTGCAGCCAC TAGCATCACT GTTTGCTGCT GCGAAGAAAC TGGGTAACGG AGCCGACATA 
CTTATCTGTG CACTTGATCT TGTCATTCGA CACAATGCAA CTCCCGATGA GGAAATTATT 
TCTGCACTGT ATGGTTTGTA TGCTACTCTG TCACCCGCTG CAGCTGCGAG TAGCGTGCAA 
TCTACTGCTA GTCGTACGTT GCTAGCTTAC GCCGTAGGCT CGACTTCCAA TGTTTCTTAT 
TATCTTTCCC CTACAACGTG TGCGCTGAGT GTTCTGTTAG GCACCGTGCG CAGGGATTGT 
TACCCACGGG TGAGTGAGGA AACGAATGCG ATATTCTTGG AGGCCATTGT GGACGTTACC 
CTGAAAATTT TAGAATCAAC GGCGAGCTCG CCGCCGTTGC AGATTCTTGG GAAATTGGTG 
AGAGTCCTAC CGTACTTCCA CCACGGTGCT GATGAAGATG CACAGAAGCA AGTTTTACGG 
CGGAAATGGC TCATTTGTCT TTCACGCGCG ACTCTCCTAT GCAGCTCCGA GGATGAACTT 
ATTCGAGAGA CGTTATCAGA TTTTCACCAT TCGCACACCA CCGCGCATCA GGAACATGCG 
GCCCACAAAC ACCTGTCAAC ATACCTATTT AGTACGGGGT ATGGCCTCCG TTTGCTTGAG 
GAGGGTCTGC GAACGAGTCT CTCGGCACAT AGCACGGATT CTTTCTCCGA CGTTAACCTT 
CTGTGTTGCG TGTTTATTTG GCCTCTCTAC AAATTTGCTA CTGCCTCTGC GCGCCAACGA 
CTTGAGGCAA CCACCATCAT TTCAAAGTTT GTTTATTCTT CAGGCCTTTT GCGGTGTCTG 
TGGGATGTGT ACCGAAATTG TCACAGCAGC ACCTTCGAAA GCACAGCTGC AATGAGCGCA 
GCGGAGCTGT TGGAGTGCGT GGCCACTGGG AAGCTGCTTG ACCTTCCTTC ACCCGTTGCC 
CCACCCACAG AGCTGCTCAG CAAGTTGTCG TTGCCTCGTT CCACGCACGG CCTGTTTTAC 
GATCCATACC CCGCTGTGTC GGTAACACTT TTTTGTCTGA TGAGTTACTT TGTTGACGCA 
ACAGGTTTCT TGGAAGGACT TGATCGCTGC GCGGTGTTTG ATCGTGCTGA CACTCTGAAG 
CTTATTTTTG CACTCAAGGG GATTTTGTAT CAGTCGTTCT TCCACGGTGT GTTGCCGTAC 
AGCAAATGTG AAGTGGTGGT GCAGGGGGCC CTCACATTGT TTTTAAAGCT GCACGTCGTA 
GGTGAGGCTC AATCGTTTGT TCCGCACCCG TCGGTTTGGA TAATTTGCCA CGATCACCTA 
CTTCTAAAGA CGTTTAAAAG TATTGAGTGT GGCACATGGT CCGCTATTGT GTCAAATGAA 
GATGATGAGG ATTGTACGGA GGATGACAAT GAAGAGCGGG AAGATGGACA TTGGGCACCA 
AGTTTGCGTG AAGGGAACCA TGAGTCGTAC GTTTCCCTTC CATGGTGGAA CGGGAGTCTT 
TCGTGGCCAC AGGAGGAACG TTGCATTCAT GTTTTGCAGC GTATCCCCTT TACCGTTCCA 
TTCGGAGCAC GTGTCACCTT GTTAACATCC TTTCTCTCTA GCCACGTGGA ACGCAGCCGT 
CCCACCACGT GGGGACACTT TGTGGTGCGT CGCGGTTGCA CTTTTGCCGA CGCTTTTGAC 
CGCTTTGCTG ATAACCCCGG TAGTAGTGAT ATGTATTATG TCCGTTTCCG TGCGGCAAAT 
GATTTGGTGG AAGAGGGTTA CGGCGACGGC GTCTACCGCG AATTTCTACT GTCCCTCTGC 
AAGGAGGGTT TTGCGGCGGA GCATGGGCTC TTTTGCTTGA CGGATGCTGG ATACGTTTAT 
CCAAATCCTT TCAGCTGGGA GGTGACTGGT GATCGGGATC ATCTCAAGCG TATCAAGTTT 
CTTGGTGCTA TGGTAGGCCG AGGATTGCGT GATGGTGTTT TGCAAGATAT TCCATTTGCT 
CTACATTTCC GTAATGCTAT TCTGGGGCGC AGCAACTCCA TCAATAACCT AAGGAGCTTT 
GATAGTCAAC TTTACCGCCA CCTTGTTTCT CTTATGAGTT TGAGCGAGGA AGAAATTGAA 
AACCTTGAGC TCAACTTCAC GTACACCGTG GAGGCCCTTG ATCGGGTGCA CGAAGTGGAG 
CTTCTACACG GTGGTCGCAA CATTGCTGTC ACTCGCCGCA ACTGTTTGAA TTATATTCAC 
TTGATTGCAG ACTTCAAACT TAATCGTGAA ACAGCCAAGC AAACACGGGC ATTTCGGAGT 
GGTCTGGAAA GTATCGTCCA CCGAAGTTGG TTACGACTCT TTGATAGTAA TGAGGTCATG 
AAGTTGTTCG GTGGTGATGT AGAGTGTAAC ATTGATGTGG AAGATTGGAA ACAGCACACT 
CAATACCACG TTCCTGACGA CGCCACTAGC AAACCCGTAC AAGTGTTCTG GGAGGTAGTG 
CAGTCTCTGC CGTTGGAACA GCAGAGGAAG CTTCTAAAGT TTTCCACTTC AATGAACCGC 
CCTCCGCTGC TTGGTTTCAA ATTTCTCAAC CCACCTTTCA AAATTCATGT CCTGTGGAAT 
GAGGCAGAAG AGCGATTGCC ATCTGCATCA ACGTGTTTCT GCACTCTAAA GCTTCCACCG 
TACCAGACGT TCGGGGTAGC GCAGCAAAAG ATAACAGCTG CAATTGAAGA GACGGATGAC 
TTCGGTCTCA GTTGA