LOC101351746 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101351746
Entrez Gene ID	101351746
Full Name	eukaryotic elongation factor 2 kinase
Synonyms	EEF2K
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004373322.2	XP_004373379.1	eukaryotic elongation factor 2 kinase
XM_004373322.1	XP_004373379.1	eukaryotic elongation factor 2 kinase

Pathways from BioSystems
Gene Ontology

The following LOC101351746 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101351746 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr169783	XM_004373322.2 Latest version!	Trichechus manatus latirostris eukaryotic elongation factor 2 kinase (LOC101351746), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OTr169783	XM_004373322.1	Trichechus manatus latirostris eukaryotic elongation factor 2 kinase (LOC101351746), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101351746 ( XM_004373322.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004373379.1 Trichechus manatus latirostris eukaryotic elongation factor 2 kinase (LOC101351746), mRNA.

Price & Availability↑

CloneID	OTr169783
Clone ID Related Accession (Same CDS sequence)	XM_004373322.2 , XM_004373322.1
Accession Version	XM_004373322.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	eukaryotic elongation factor 2 kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443960.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_004373322.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG AAGACCTCAT CTTCCGCCTG GAAGGCGTTG ATGGTAGCCA GACCTCCCGG 
GCTGGCCACG ATGGCAATTC TGACACAGAC AGCGATGATG AGGAAGGTTT CTTTATCTGC 
CCCATCACTG ATGACCCCAG ATCTAACCAG AATGCCAATT CCAAGGTTAA TAACTACTAC 
AGCAACCTAA CAAAAAGTGA GCAGTATGGG TCCAGCGGGT CCCCGGCAAG TTCCTTCCAC 
TTTAAGGAAG CCTGGAAGCA TGCGATCGAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TCGAGGACAT TGCCACAGAA TGTGCGATAC GTCACAGGTA CAACGCTGTC 
ACGGGGGAGT GGCTGCAAGA CGAAGTTCTG ATCAAGATGG CGTCTCAGCC CTTTGGCCGT 
GGAGCGATGA GGGAGTGCTT TAGGACGAAG AAGCTCTCCA ACTTCCTGCA CACCCGGCAG 
TGGAAGGGGG CCTCCAACTA TGTGGCAAAG CGCTACATCG AGCCCGTTGG CAGGGATGTG 
TACTTAGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAACCGC 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCC TCATTGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTTTTCCA CCTGGAACAC TACATTGAGG GCAAGTACAT CAAATACAAT 
TCCAACTCGG GCTTTGTCCG TGACGACAAC ATCCGGCTGA CACCACAGGC CTTCAGCCAC 
TTCACGTTTG AGCGTTCCGG CCATCAGCTA ATTGTGGTGG ACATCCAGGG TGTGGGCGAC 
CTCTACACCG ACCCGCAGAT CCACACCGAG ACAGGCGCTG ACTTCGGAGA CGGCAACCTA 
GGGGTCCGAG GGATGGCTCT ATTCTTCTAT TCACATGCCT GCAACCGGAT CTGCAAGAGC 
ATGGGCCTCA CACCCTTTGA CCTCTCGCCA AAGGAGAGGG ACACCGTGAA TCAGAGCACC 
AAACTGCTGC AATCAGCCAA GACCATCTTG AGGGGGACAG AAGAAAAATG TGGGAGCCCC 
CGAGTAAGGA CCCTTTCTGG GAGCCGGCCC CCCCTGCTCC TTCGCCTTTC AGAGAACTCT 
GGAGACGAGA ATATGAGTGA TGTAACCTTT GACTCTCTGC CTTCCTCACC GTCTTCGGCC 
ACACCACACA GCCAGAAACT AGACAACCTC CATTGGCCGG TGTTCTGCGA CCTTGATAAT 
CTGGCACACA GAGACCACGA TCATCTGGAC AACCATCGGG ACTCTGAGAA CAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CAGAGCCCCA AGAACACGGC 
CACTCAAACG GTAATCGGAG GTACGAGTCT GATGAAGACA GCCTGGGCAG CTCCGGACGG 
GTTTGTGTGG AGCAGTGGAA TCTCCTTAAC TCCTCCCGCC TCCACCTGTC GAGACCTTCG 
GCTGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TTGAAAAGAA AATCGGGAAG 
TCTATTTTGG GGAAGGTGCA TCTGGCCATG GTGCGGTACC ATGAAGGCGG GCGCTTCTGT 
GAGAAGGATG AGGAGTGGGA CCGTGAGTCG GCAGTCTTCC ACCTGGAGCA CGCGGCCGAC 
CTGGGCGAGC TGGAGGCCAT TGTGGGTCTG GGGCTCATGT ACTCGCAGCT GCCCCACCAC 
ATTCTGGCTG ACGTCTCTCT GAAGGAGACG GAAGAGAATA AAACAAAAGG ATTCGATTAC 
TTACTGAAGG CAGCTGAAGC TGGCGACAGA CAGTCTATGA TCGTGGTGGC GCGAGCTTTT 
GACTCTGGCC AGAACCTTAG CCCAGACAGG TCCCGAGACT GGTCAGAGGC CCTGCACTGG 
TACAACACTG CCCTAGAGAC CACAGACTGT GATGAGGGTG GTGAATATGA TGGGATGCAA 
GATGAGCCCC GGTACATGCT GCTGGCCAGG GAGGCCGAGA TGCTGTTTAC GGGTGGCTGC 
GGGCTGGAGA AGGACCTACA GAGATCAGGC GACTTGTACA CAGAGGCAGC GGAAGCAGCT 
ATGGAAGCCA TGAAGGGCCG GCTGGCCAAC CAGTACTACC AGAAGGCGGA GGAGGCCTGG 
GCCCAGATGG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004373379.1
CDS	190..2367
Translation	MADEDLIFRLEGVDGSQTSRAGHDGNSDTDSDDEEGFFICPITDDPRSNQNANSKVNNYYSNLTKSEQYGSSGSPASSFHFKEAWKHAIEKAKHMPDPWAEFHLEDIATECAIRHRYNAVTGEWLQDEVLIKMASQPFGRGAMRECFRTKKLSNFLHTRQWKGASNYVAKRYIEPVGRDVYLEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCLIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGADFGDGNLGVRGMALFFYSHACNRICKSMGLTPFDLSPKERDTVNQSTKLLQSAKTILRGTEEKCGSPRVRTLSGSRPPLLLRLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDNLHWPVFCDLDNLAHRDHDHLDNHRDSENSGDSGYPSEKRGELDDPEPQEHGHSNGNRRYESDEDSLGSSGRVCVEQWNLLNSSRLHLSRPSAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKDEEWDRESAVFHLEHAADLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMIVVARAFDSGQNLSPDRSRDWSEALHWYNTALETTDCDEGGEYDGMQDEPRYMLLAREAEMLFTGGCGLEKDLQRSGDLYTEAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

Target ORF information:

RefSeq Version	XM_004373322.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris eukaryotic elongation factor 2 kinase (LOC101351746), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004373322.2

ORF Insert Sequence:

ATGGCAGATG AAGACCTCAT CTTCCGCCTG GAAGGCGTTG ATGGTAGCCA GACCTCCCGG 
GCTGGCCACG ATGGCAATTC TGACACAGAC AGCGATGATG AGGAAGGTTT CTTTATCTGC 
CCCATCACTG ATGACCCCAG ATCTAACCAG AATGCCAATT CCAAGGTTAA TAACTACTAC 
AGCAACCTAA CAAAAAGTGA GCAGTATGGG TCCAGCGGGT CCCCGGCAAG TTCCTTCCAC 
TTTAAGGAAG CCTGGAAGCA TGCGATCGAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TCGAGGACAT TGCCACAGAA TGTGCGATAC GTCACAGGTA CAACGCTGTC 
ACGGGGGAGT GGCTGCAAGA CGAAGTTCTG ATCAAGATGG CGTCTCAGCC CTTTGGCCGT 
GGAGCGATGA GGGAGTGCTT TAGGACGAAG AAGCTCTCCA ACTTCCTGCA CACCCGGCAG 
TGGAAGGGGG CCTCCAACTA TGTGGCAAAG CGCTACATCG AGCCCGTTGG CAGGGATGTG 
TACTTAGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAACCGC 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCC TCATTGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTTTTCCA CCTGGAACAC TACATTGAGG GCAAGTACAT CAAATACAAT 
TCCAACTCGG GCTTTGTCCG TGACGACAAC ATCCGGCTGA CACCACAGGC CTTCAGCCAC 
TTCACGTTTG AGCGTTCCGG CCATCAGCTA ATTGTGGTGG ACATCCAGGG TGTGGGCGAC 
CTCTACACCG ACCCGCAGAT CCACACCGAG ACAGGCGCTG ACTTCGGAGA CGGCAACCTA 
GGGGTCCGAG GGATGGCTCT ATTCTTCTAT TCACATGCCT GCAACCGGAT CTGCAAGAGC 
ATGGGCCTCA CACCCTTTGA CCTCTCGCCA AAGGAGAGGG ACACCGTGAA TCAGAGCACC 
AAACTGCTGC AATCAGCCAA GACCATCTTG AGGGGGACAG AAGAAAAATG TGGGAGCCCC 
CGAGTAAGGA CCCTTTCTGG GAGCCGGCCC CCCCTGCTCC TTCGCCTTTC AGAGAACTCT 
GGAGACGAGA ATATGAGTGA TGTAACCTTT GACTCTCTGC CTTCCTCACC GTCTTCGGCC 
ACACCACACA GCCAGAAACT AGACAACCTC CATTGGCCGG TGTTCTGCGA CCTTGATAAT 
CTGGCACACA GAGACCACGA TCATCTGGAC AACCATCGGG ACTCTGAGAA CAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CAGAGCCCCA AGAACACGGC 
CACTCAAACG GTAATCGGAG GTACGAGTCT GATGAAGACA GCCTGGGCAG CTCCGGACGG 
GTTTGTGTGG AGCAGTGGAA TCTCCTTAAC TCCTCCCGCC TCCACCTGTC GAGACCTTCG 
GCTGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TTGAAAAGAA AATCGGGAAG 
TCTATTTTGG GGAAGGTGCA TCTGGCCATG GTGCGGTACC ATGAAGGCGG GCGCTTCTGT 
GAGAAGGATG AGGAGTGGGA CCGTGAGTCG GCAGTCTTCC ACCTGGAGCA CGCGGCCGAC 
CTGGGCGAGC TGGAGGCCAT TGTGGGTCTG GGGCTCATGT ACTCGCAGCT GCCCCACCAC 
ATTCTGGCTG ACGTCTCTCT GAAGGAGACG GAAGAGAATA AAACAAAAGG ATTCGATTAC 
TTACTGAAGG CAGCTGAAGC TGGCGACAGA CAGTCTATGA TCGTGGTGGC GCGAGCTTTT 
GACTCTGGCC AGAACCTTAG CCCAGACAGG TCCCGAGACT GGTCAGAGGC CCTGCACTGG 
TACAACACTG CCCTAGAGAC CACAGACTGT GATGAGGGTG GTGAATATGA TGGGATGCAA 
GATGAGCCCC GGTACATGCT GCTGGCCAGG GAGGCCGAGA TGCTGTTTAC GGGTGGCTGC 
GGGCTGGAGA AGGACCTACA GAGATCAGGC GACTTGTACA CAGAGGCAGC GGAAGCAGCT 
ATGGAAGCCA TGAAGGGCCG GCTGGCCAAC CAGTACTACC AGAAGGCGGA GGAGGCCTGG 
GCCCAGATGG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101351746 ( XM_004373322.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004373379.1 Trichechus manatus latirostris eukaryotic elongation factor 2 kinase (LOC101351746), mRNA.

Price & Availability↑

CloneID	OTr169783
Clone ID Related Accession (Same CDS sequence)	XM_004373322.2 , XM_004373322.1
Accession Version	XM_004373322.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	eukaryotic elongation factor 2 kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443960.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGATG AAGACCTCAT CTTCCGCCTG GAAGGCGTTG ATGGTAGCCA GACCTCCCGG 
GCTGGCCACG ATGGCAATTC TGACACAGAC AGCGATGATG AGGAAGGTTT CTTTATCTGC 
CCCATCACTG ATGACCCCAG ATCTAACCAG AATGCCAATT CCAAGGTTAA TAACTACTAC 
AGCAACCTAA CAAAAAGTGA GCAGTATGGG TCCAGCGGGT CCCCGGCAAG TTCCTTCCAC 
TTTAAGGAAG CCTGGAAGCA TGCGATCGAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TCGAGGACAT TGCCACAGAA TGTGCGATAC GTCACAGGTA CAACGCTGTC 
ACGGGGGAGT GGCTGCAAGA CGAAGTTCTG ATCAAGATGG CGTCTCAGCC CTTTGGCCGT 
GGAGCGATGA GGGAGTGCTT TAGGACGAAG AAGCTCTCCA ACTTCCTGCA CACCCGGCAG 
TGGAAGGGGG CCTCCAACTA TGTGGCAAAG CGCTACATCG AGCCCGTTGG CAGGGATGTG 
TACTTAGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAACCGC 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCC TCATTGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTTTTCCA CCTGGAACAC TACATTGAGG GCAAGTACAT CAAATACAAT 
TCCAACTCGG GCTTTGTCCG TGACGACAAC ATCCGGCTGA CACCACAGGC CTTCAGCCAC 
TTCACGTTTG AGCGTTCCGG CCATCAGCTA ATTGTGGTGG ACATCCAGGG TGTGGGCGAC 
CTCTACACCG ACCCGCAGAT CCACACCGAG ACAGGCGCTG ACTTCGGAGA CGGCAACCTA 
GGGGTCCGAG GGATGGCTCT ATTCTTCTAT TCACATGCCT GCAACCGGAT CTGCAAGAGC 
ATGGGCCTCA CACCCTTTGA CCTCTCGCCA AAGGAGAGGG ACACCGTGAA TCAGAGCACC 
AAACTGCTGC AATCAGCCAA GACCATCTTG AGGGGGACAG AAGAAAAATG TGGGAGCCCC 
CGAGTAAGGA CCCTTTCTGG GAGCCGGCCC CCCCTGCTCC TTCGCCTTTC AGAGAACTCT 
GGAGACGAGA ATATGAGTGA TGTAACCTTT GACTCTCTGC CTTCCTCACC GTCTTCGGCC 
ACACCACACA GCCAGAAACT AGACAACCTC CATTGGCCGG TGTTCTGCGA CCTTGATAAT 
CTGGCACACA GAGACCACGA TCATCTGGAC AACCATCGGG ACTCTGAGAA CAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CAGAGCCCCA AGAACACGGC 
CACTCAAACG GTAATCGGAG GTACGAGTCT GATGAAGACA GCCTGGGCAG CTCCGGACGG 
GTTTGTGTGG AGCAGTGGAA TCTCCTTAAC TCCTCCCGCC TCCACCTGTC GAGACCTTCG 
GCTGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TTGAAAAGAA AATCGGGAAG 
TCTATTTTGG GGAAGGTGCA TCTGGCCATG GTGCGGTACC ATGAAGGCGG GCGCTTCTGT 
GAGAAGGATG AGGAGTGGGA CCGTGAGTCG GCAGTCTTCC ACCTGGAGCA CGCGGCCGAC 
CTGGGCGAGC TGGAGGCCAT TGTGGGTCTG GGGCTCATGT ACTCGCAGCT GCCCCACCAC 
ATTCTGGCTG ACGTCTCTCT GAAGGAGACG GAAGAGAATA AAACAAAAGG ATTCGATTAC 
TTACTGAAGG CAGCTGAAGC TGGCGACAGA CAGTCTATGA TCGTGGTGGC GCGAGCTTTT 
GACTCTGGCC AGAACCTTAG CCCAGACAGG TCCCGAGACT GGTCAGAGGC CCTGCACTGG 
TACAACACTG CCCTAGAGAC CACAGACTGT GATGAGGGTG GTGAATATGA TGGGATGCAA 
GATGAGCCCC GGTACATGCT GCTGGCCAGG GAGGCCGAGA TGCTGTTTAC GGGTGGCTGC 
GGGCTGGAGA AGGACCTACA GAGATCAGGC GACTTGTACA CAGAGGCAGC GGAAGCAGCT 
ATGGAAGCCA TGAAGGGCCG GCTGGCCAAC CAGTACTACC AGAAGGCGGA GGAGGCCTGG 
GCCCAGATGG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004373379.1
CDS	155..2332
Translation	MADEDLIFRLEGVDGSQTSRAGHDGNSDTDSDDEEGFFICPITDDPRSNQNANSKVNNYYSNLTKSEQYGSSGSPASSFHFKEAWKHAIEKAKHMPDPWAEFHLEDIATECAIRHRYNAVTGEWLQDEVLIKMASQPFGRGAMRECFRTKKLSNFLHTRQWKGASNYVAKRYIEPVGRDVYLEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCLIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGADFGDGNLGVRGMALFFYSHACNRICKSMGLTPFDLSPKERDTVNQSTKLLQSAKTILRGTEEKCGSPRVRTLSGSRPPLLLRLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDNLHWPVFCDLDNLAHRDHDHLDNHRDSENSGDSGYPSEKRGELDDPEPQEHGHSNGNRRYESDEDSLGSSGRVCVEQWNLLNSSRLHLSRPSAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKDEEWDRESAVFHLEHAADLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMIVVARAFDSGQNLSPDRSRDWSEALHWYNTALETTDCDEGGEYDGMQDEPRYMLLAREAEMLFTGGCGLEKDLQRSGDLYTEAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

Target ORF information:

RefSeq Version	XM_004373322.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris eukaryotic elongation factor 2 kinase (LOC101351746), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004373322.1

ORF Insert Sequence:

ATGGCAGATG AAGACCTCAT CTTCCGCCTG GAAGGCGTTG ATGGTAGCCA GACCTCCCGG 
GCTGGCCACG ATGGCAATTC TGACACAGAC AGCGATGATG AGGAAGGTTT CTTTATCTGC 
CCCATCACTG ATGACCCCAG ATCTAACCAG AATGCCAATT CCAAGGTTAA TAACTACTAC 
AGCAACCTAA CAAAAAGTGA GCAGTATGGG TCCAGCGGGT CCCCGGCAAG TTCCTTCCAC 
TTTAAGGAAG CCTGGAAGCA TGCGATCGAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT 
GAGTTCCACC TCGAGGACAT TGCCACAGAA TGTGCGATAC GTCACAGGTA CAACGCTGTC 
ACGGGGGAGT GGCTGCAAGA CGAAGTTCTG ATCAAGATGG CGTCTCAGCC CTTTGGCCGT 
GGAGCGATGA GGGAGTGCTT TAGGACGAAG AAGCTCTCCA ACTTCCTGCA CACCCGGCAG 
TGGAAGGGGG CCTCCAACTA TGTGGCAAAG CGCTACATCG AGCCCGTTGG CAGGGATGTG 
TACTTAGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAACCGC 
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCC TCATTGAGCT GAAGGACAGA 
CCGGGCAAGC CCCTTTTCCA CCTGGAACAC TACATTGAGG GCAAGTACAT CAAATACAAT 
TCCAACTCGG GCTTTGTCCG TGACGACAAC ATCCGGCTGA CACCACAGGC CTTCAGCCAC 
TTCACGTTTG AGCGTTCCGG CCATCAGCTA ATTGTGGTGG ACATCCAGGG TGTGGGCGAC 
CTCTACACCG ACCCGCAGAT CCACACCGAG ACAGGCGCTG ACTTCGGAGA CGGCAACCTA 
GGGGTCCGAG GGATGGCTCT ATTCTTCTAT TCACATGCCT GCAACCGGAT CTGCAAGAGC 
ATGGGCCTCA CACCCTTTGA CCTCTCGCCA AAGGAGAGGG ACACCGTGAA TCAGAGCACC 
AAACTGCTGC AATCAGCCAA GACCATCTTG AGGGGGACAG AAGAAAAATG TGGGAGCCCC 
CGAGTAAGGA CCCTTTCTGG GAGCCGGCCC CCCCTGCTCC TTCGCCTTTC AGAGAACTCT 
GGAGACGAGA ATATGAGTGA TGTAACCTTT GACTCTCTGC CTTCCTCACC GTCTTCGGCC 
ACACCACACA GCCAGAAACT AGACAACCTC CATTGGCCGG TGTTCTGCGA CCTTGATAAT 
CTGGCACACA GAGACCACGA TCATCTGGAC AACCATCGGG ACTCTGAGAA CAGTGGGGAC 
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CAGAGCCCCA AGAACACGGC 
CACTCAAACG GTAATCGGAG GTACGAGTCT GATGAAGACA GCCTGGGCAG CTCCGGACGG 
GTTTGTGTGG AGCAGTGGAA TCTCCTTAAC TCCTCCCGCC TCCACCTGTC GAGACCTTCG 
GCTGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TTGAAAAGAA AATCGGGAAG 
TCTATTTTGG GGAAGGTGCA TCTGGCCATG GTGCGGTACC ATGAAGGCGG GCGCTTCTGT 
GAGAAGGATG AGGAGTGGGA CCGTGAGTCG GCAGTCTTCC ACCTGGAGCA CGCGGCCGAC 
CTGGGCGAGC TGGAGGCCAT TGTGGGTCTG GGGCTCATGT ACTCGCAGCT GCCCCACCAC 
ATTCTGGCTG ACGTCTCTCT GAAGGAGACG GAAGAGAATA AAACAAAAGG ATTCGATTAC 
TTACTGAAGG CAGCTGAAGC TGGCGACAGA CAGTCTATGA TCGTGGTGGC GCGAGCTTTT 
GACTCTGGCC AGAACCTTAG CCCAGACAGG TCCCGAGACT GGTCAGAGGC CCTGCACTGG 
TACAACACTG CCCTAGAGAC CACAGACTGT GATGAGGGTG GTGAATATGA TGGGATGCAA 
GATGAGCCCC GGTACATGCT GCTGGCCAGG GAGGCCGAGA TGCTGTTTAC GGGTGGCTGC 
GGGCTGGAGA AGGACCTACA GAGATCAGGC GACTTGTACA CAGAGGCAGC GGAAGCAGCT 
ATGGAAGCCA TGAAGGGCCG GCTGGCCAAC CAGTACTACC AGAAGGCGGA GGAGGCCTGG 
GCCCAGATGG AGGAATGA