LOC101351282 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101351282
Entrez Gene ID	101351282
Full Name	5'-aminolevulinate synthase 1
Synonyms	ALAS1
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023727154.1	XP_023582922.1	5-aminolevulinate synthase, nonspecific, mitochondrial isoform X1
XM_004368595.1	XP_004368652.1	5-aminolevulinate synthase, nonspecific, mitochondrial
XM_023727172.1	XP_023582940.1	5-aminolevulinate synthase, nonspecific, mitochondrial isoform X2
XM_023727180.1	XP_023582948.1	5-aminolevulinate synthase, nonspecific, mitochondrial isoform X3
XM_023727163.1	XP_023582931.1	5-aminolevulinate synthase, nonspecific, mitochondrial isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101351282 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101351282 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr164471	XM_004368595.1 Latest version!	Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OTr164471	XM_023727163.1 Latest version!	Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OTr164471	XM_023727154.1 Latest version!	Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OTr214082	XM_023727172.1 Latest version!	Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OTr214083	XM_023727180.1 Latest version!	Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101351282 ( XM_023727154.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023582922.1 Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTr164471
Clone ID Related Accession (Same CDS sequence)	XM_023727154.1 , XM_004368595.1 , XM_023727163.1
Accession Version	XM_023727154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	5-aminolevulinate synthase, nonspecific, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443937.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG TTGTTCGCCG CTGCCCATTT TTATCCCGAG TGCCCCAGAC CTTTCTGCAG 
AAGGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGCTGGG 
GCCAAGCCAG CCCCCCGGGC CCTGTCTACT TCAGCAGTTC ACTGCCAGCA GGTCAAAGAA 
ACCCCTCCAG CCAATGAAAA GGACAAAACT GCCAAAGCCG AGGTCCAGCA GGCTTCTGAT 
GGATCCCAGA AGACTCCAGA TGGGGCGCAG CTTCCGTCTG AACACCTCTC CCCTGCCACG 
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTAGCAGCCC AGATGAGCCA GAGGGGCAGC 
AGTGTCTTCC GCAAAGCCAG TCTCGAGCTT CAGGAGGATG TGCAGGAAAT GCATGCCTTG 
AGGAAAGAGG TTGTCCAAAC CTCTGTGAAC CCCCGTGTAA TTAGTGTGAA GACCGATGGC 
GAGGAGCCAA GTGGATTGCT GAAGAACTTC CAAGATATCA TGCGAAAGCA AAGACCAGAA 
AGAGTGTCCC ATCTTCTTCA AGATAATTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT 
CGTTTCTTTG AGAAGAAAAT TGATGAGAAA AAAAATGACC ACACCTATCG AGTTTTCAAA 
ACCGTGAACC GGAGGGCACA CATCTTCCCC ATGGCGGATG ACTACTCAGA CTCCCTCATC 
ACCAAAAAGC AGGTGTCGGT TTGGTGTAGC AATGACTACC TAGGAATGAG TCGCCACCCA 
CGGGTGTGTG GGGCAGTCAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGCACT 
AGAAATATTT CTGGAACCAG TAAATTCCAC GTGGACCTGG AGCAGGAGCT TGCTGACCTC 
CATGGGAAAG ATGCAGCACT CCTGTTTTCC TCATGCTTTG TAGCCAATGA CTCGACCCTC 
TTTACCTTGG CTAAGATGAT GCCTGGCTGT GAAATTTACT CTGATGCTGG GAACCACGCC 
TCTATGATCC AGGGGATTCG GAACAGCCGA GTGCCAAAGT ATATCTTCCG CCACAATGAT 
GTCAGCCATC TCAAAGAACT GCTGCAAAGA TCTGACCCTT CCGTCCCCAA GATTGTTGCA 
TTTGAAACCG TCCACTCAAT GGATGGGGCG GTGTGTCCAC TGGAAGAGCT GTGTGACGTT 
GCCCACGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCGGTGGG GCTGTACGGG 
GCTCGAGGCG GAGGGATTGG CGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT 
GGAACACTTG GCAAAGCCTT CGGCTGTGTT GGCGGCTACA TCGCCAGCAC GAGTTCTCTG 
GTTGACACCG TGCGGTCCTA TGCTGCGGGC TTCATCTTCA CCACCTCCCT GCCGCCCATG 
CTGCTGGCTG GAGCTCTGGA GTCTGTGCGG ATCCTGAAGA GCACTGAGGG GCGGGCACTT 
CGCCGCCAGC ACCAGCGCAA CGTCAAGCTC ATGAGGCAGA TGCTAATGGA TGCCGGCCTC 
CCAGTGGTCC ACTGCCCCAG TCACATCATC CCTGTCCGGG TTGCAGATGC TGCTAAAAAC 
ACGGAAGTCT GTGACGAGCT AATGAGCAGA CATAACATCT ATGTCCAAGC AATCAATTAC 
CCGACAGTGC CCCGCGGGGA GGAGCTCCTG CGGATCGCCC CCACCCCTCA CCACACACCA 
CAGATGATGA ACTACTTCCT TGAGAATCTT CTGGCCACGT GGAACCGAGT GGCGCTGGAG 
CTGAAACCAC ATTCCTCAGC TGAGTGTAAC TTCTGTAGGA GGCCGCTGCA TTTCGAAGTG 
ATGAGTGAAA GAGAGAAATC CTACTTCTCA GGCATGAGCA AGCTGGTTTC TGCTCAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023582922.1
CDS	397..2319
Translation	METVVRRCPFLSRVPQTFLQKAGKSLLFYAQNCPKMMEAGAKPAPRALSTSAVHCQQVKETPPANEKDKTAKAEVQQASDGSQKTPDGAQLPSEHLSPATSQGTASKCPFLAAQMSQRGSSVFRKASLELQEDVQEMHALRKEVVQTSVNPRVISVKTDGEEPSGLLKNFQDIMRKQRPERVSHLLQDNLPKSVSTFQYDRFFEKKIDEKKNDHTYRVFKTVNRRAHIFPMADDYSDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMDTLKQHGAGAGGTRNISGTSKFHVDLEQELADLHGKDAALLFSSCFVANDSTLFTLAKMMPGCEIYSDAGNHASMIQGIRNSRVPKYIFRHNDVSHLKELLQRSDPSVPKIVAFETVHSMDGAVCPLEELCDVAHEFGAITFVDEVHAVGLYGARGGGIGDRDGVMPKMDIISGTLGKAFGCVGGYIASTSSLVDTVRSYAAGFIFTTSLPPMLLAGALESVRILKSTEGRALRRQHQRNVKLMRQMLMDAGLPVVHCPSHIIPVRVADAAKNTEVCDELMSRHNIYVQAINYPTVPRGEELLRIAPTPHHTPQMMNYFLENLLATWNRVALELKPHSSAECNFCRRPLHFEVMSEREKSYFSGMSKLVSAQA

Target ORF information:

RefSeq Version	XM_023727154.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023727154.1

ORF Insert Sequence:

ATGGAGACTG TTGTTCGCCG CTGCCCATTT TTATCCCGAG TGCCCCAGAC CTTTCTGCAG 
AAGGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGCTGGG 
GCCAAGCCAG CCCCCCGGGC CCTGTCTACT TCAGCAGTTC ACTGCCAGCA GGTCAAAGAA 
ACCCCTCCAG CCAATGAAAA GGACAAAACT GCCAAAGCCG AGGTCCAGCA GGCTTCTGAT 
GGATCCCAGA AGACTCCAGA TGGGGCGCAG CTTCCGTCTG AACACCTCTC CCCTGCCACG 
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTAGCAGCCC AGATGAGCCA GAGGGGCAGC 
AGTGTCTTCC GCAAAGCCAG TCTCGAGCTT CAGGAGGATG TGCAGGAAAT GCATGCCTTG 
AGGAAAGAGG TTGTCCAAAC CTCTGTGAAC CCCCGTGTAA TTAGTGTGAA GACCGATGGC 
GAGGAGCCAA GTGGATTGCT GAAGAACTTC CAAGATATCA TGCGAAAGCA AAGACCAGAA 
AGAGTGTCCC ATCTTCTTCA AGATAATTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT 
CGTTTCTTTG AGAAGAAAAT TGATGAGAAA AAAAATGACC ACACCTATCG AGTTTTCAAA 
ACCGTGAACC GGAGGGCACA CATCTTCCCC ATGGCGGATG ACTACTCAGA CTCCCTCATC 
ACCAAAAAGC AGGTGTCGGT TTGGTGTAGC AATGACTACC TAGGAATGAG TCGCCACCCA 
CGGGTGTGTG GGGCAGTCAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGCACT 
AGAAATATTT CTGGAACCAG TAAATTCCAC GTGGACCTGG AGCAGGAGCT TGCTGACCTC 
CATGGGAAAG ATGCAGCACT CCTGTTTTCC TCATGCTTTG TAGCCAATGA CTCGACCCTC 
TTTACCTTGG CTAAGATGAT GCCTGGCTGT GAAATTTACT CTGATGCTGG GAACCACGCC 
TCTATGATCC AGGGGATTCG GAACAGCCGA GTGCCAAAGT ATATCTTCCG CCACAATGAT 
GTCAGCCATC TCAAAGAACT GCTGCAAAGA TCTGACCCTT CCGTCCCCAA GATTGTTGCA 
TTTGAAACCG TCCACTCAAT GGATGGGGCG GTGTGTCCAC TGGAAGAGCT GTGTGACGTT 
GCCCACGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCGGTGGG GCTGTACGGG 
GCTCGAGGCG GAGGGATTGG CGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT 
GGAACACTTG GCAAAGCCTT CGGCTGTGTT GGCGGCTACA TCGCCAGCAC GAGTTCTCTG 
GTTGACACCG TGCGGTCCTA TGCTGCGGGC TTCATCTTCA CCACCTCCCT GCCGCCCATG 
CTGCTGGCTG GAGCTCTGGA GTCTGTGCGG ATCCTGAAGA GCACTGAGGG GCGGGCACTT 
CGCCGCCAGC ACCAGCGCAA CGTCAAGCTC ATGAGGCAGA TGCTAATGGA TGCCGGCCTC 
CCAGTGGTCC ACTGCCCCAG TCACATCATC CCTGTCCGGG TTGCAGATGC TGCTAAAAAC 
ACGGAAGTCT GTGACGAGCT AATGAGCAGA CATAACATCT ATGTCCAAGC AATCAATTAC 
CCGACAGTGC CCCGCGGGGA GGAGCTCCTG CGGATCGCCC CCACCCCTCA CCACACACCA 
CAGATGATGA ACTACTTCCT TGAGAATCTT CTGGCCACGT GGAACCGAGT GGCGCTGGAG 
CTGAAACCAC ATTCCTCAGC TGAGTGTAAC TTCTGTAGGA GGCCGCTGCA TTTCGAAGTG 
ATGAGTGAAA GAGAGAAATC CTACTTCTCA GGCATGAGCA AGCTGGTTTC TGCTCAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101351282 ( XM_004368595.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004368652.1 Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), mRNA.

Price & Availability↑

CloneID	OTr164471
Clone ID Related Accession (Same CDS sequence)	XM_023727154.1 , XM_004368595.1 , XM_023727163.1
Accession Version	XM_004368595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	5-aminolevulinate synthase, nonspecific, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443937.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG TTGTTCGCCG CTGCCCATTT TTATCCCGAG TGCCCCAGAC CTTTCTGCAG 
AAGGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGCTGGG 
GCCAAGCCAG CCCCCCGGGC CCTGTCTACT TCAGCAGTTC ACTGCCAGCA GGTCAAAGAA 
ACCCCTCCAG CCAATGAAAA GGACAAAACT GCCAAAGCCG AGGTCCAGCA GGCTTCTGAT 
GGATCCCAGA AGACTCCAGA TGGGGCGCAG CTTCCGTCTG AACACCTCTC CCCTGCCACG 
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTAGCAGCCC AGATGAGCCA GAGGGGCAGC 
AGTGTCTTCC GCAAAGCCAG TCTCGAGCTT CAGGAGGATG TGCAGGAAAT GCATGCCTTG 
AGGAAAGAGG TTGTCCAAAC CTCTGTGAAC CCCCGTGTAA TTAGTGTGAA GACCGATGGC 
GAGGAGCCAA GTGGATTGCT GAAGAACTTC CAAGATATCA TGCGAAAGCA AAGACCAGAA 
AGAGTGTCCC ATCTTCTTCA AGATAATTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT 
CGTTTCTTTG AGAAGAAAAT TGATGAGAAA AAAAATGACC ACACCTATCG AGTTTTCAAA 
ACCGTGAACC GGAGGGCACA CATCTTCCCC ATGGCGGATG ACTACTCAGA CTCCCTCATC 
ACCAAAAAGC AGGTGTCGGT TTGGTGTAGC AATGACTACC TAGGAATGAG TCGCCACCCA 
CGGGTGTGTG GGGCAGTCAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGCACT 
AGAAATATTT CTGGAACCAG TAAATTCCAC GTGGACCTGG AGCAGGAGCT TGCTGACCTC 
CATGGGAAAG ATGCAGCACT CCTGTTTTCC TCATGCTTTG TAGCCAATGA CTCGACCCTC 
TTTACCTTGG CTAAGATGAT GCCTGGCTGT GAAATTTACT CTGATGCTGG GAACCACGCC 
TCTATGATCC AGGGGATTCG GAACAGCCGA GTGCCAAAGT ATATCTTCCG CCACAATGAT 
GTCAGCCATC TCAAAGAACT GCTGCAAAGA TCTGACCCTT CCGTCCCCAA GATTGTTGCA 
TTTGAAACCG TCCACTCAAT GGATGGGGCG GTGTGTCCAC TGGAAGAGCT GTGTGACGTT 
GCCCACGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCGGTGGG GCTGTACGGG 
GCTCGAGGCG GAGGGATTGG CGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT 
GGAACACTTG GCAAAGCCTT CGGCTGTGTT GGCGGCTACA TCGCCAGCAC GAGTTCTCTG 
GTTGACACCG TGCGGTCCTA TGCTGCGGGC TTCATCTTCA CCACCTCCCT GCCGCCCATG 
CTGCTGGCTG GAGCTCTGGA GTCTGTGCGG ATCCTGAAGA GCACTGAGGG GCGGGCACTT 
CGCCGCCAGC ACCAGCGCAA CGTCAAGCTC ATGAGGCAGA TGCTAATGGA TGCCGGCCTC 
CCAGTGGTCC ACTGCCCCAG TCACATCATC CCTGTCCGGG TTGCAGATGC TGCTAAAAAC 
ACGGAAGTCT GTGACGAGCT AATGAGCAGA CATAACATCT ATGTCCAAGC AATCAATTAC 
CCGACAGTGC CCCGCGGGGA GGAGCTCCTG CGGATCGCCC CCACCCCTCA CCACACACCA 
CAGATGATGA ACTACTTCCT TGAGAATCTT CTGGCCACGT GGAACCGAGT GGCGCTGGAG 
CTGAAACCAC ATTCCTCAGC TGAGTGTAAC TTCTGTAGGA GGCCGCTGCA TTTCGAAGTG 
ATGAGTGAAA GAGAGAAATC CTACTTCTCA GGCATGAGCA AGCTGGTTTC TGCTCAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004368652.1
CDS	178..2100
Translation	METVVRRCPFLSRVPQTFLQKAGKSLLFYAQNCPKMMEAGAKPAPRALSTSAVHCQQVKETPPANEKDKTAKAEVQQASDGSQKTPDGAQLPSEHLSPATSQGTASKCPFLAAQMSQRGSSVFRKASLELQEDVQEMHALRKEVVQTSVNPRVISVKTDGEEPSGLLKNFQDIMRKQRPERVSHLLQDNLPKSVSTFQYDRFFEKKIDEKKNDHTYRVFKTVNRRAHIFPMADDYSDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMDTLKQHGAGAGGTRNISGTSKFHVDLEQELADLHGKDAALLFSSCFVANDSTLFTLAKMMPGCEIYSDAGNHASMIQGIRNSRVPKYIFRHNDVSHLKELLQRSDPSVPKIVAFETVHSMDGAVCPLEELCDVAHEFGAITFVDEVHAVGLYGARGGGIGDRDGVMPKMDIISGTLGKAFGCVGGYIASTSSLVDTVRSYAAGFIFTTSLPPMLLAGALESVRILKSTEGRALRRQHQRNVKLMRQMLMDAGLPVVHCPSHIIPVRVADAAKNTEVCDELMSRHNIYVQAINYPTVPRGEELLRIAPTPHHTPQMMNYFLENLLATWNRVALELKPHSSAECNFCRRPLHFEVMSEREKSYFSGMSKLVSAQA

Target ORF information:

RefSeq Version	XM_004368595.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004368595.1

ORF Insert Sequence:

ATGGAGACTG TTGTTCGCCG CTGCCCATTT TTATCCCGAG TGCCCCAGAC CTTTCTGCAG 
AAGGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGCTGGG 
GCCAAGCCAG CCCCCCGGGC CCTGTCTACT TCAGCAGTTC ACTGCCAGCA GGTCAAAGAA 
ACCCCTCCAG CCAATGAAAA GGACAAAACT GCCAAAGCCG AGGTCCAGCA GGCTTCTGAT 
GGATCCCAGA AGACTCCAGA TGGGGCGCAG CTTCCGTCTG AACACCTCTC CCCTGCCACG 
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTAGCAGCCC AGATGAGCCA GAGGGGCAGC 
AGTGTCTTCC GCAAAGCCAG TCTCGAGCTT CAGGAGGATG TGCAGGAAAT GCATGCCTTG 
AGGAAAGAGG TTGTCCAAAC CTCTGTGAAC CCCCGTGTAA TTAGTGTGAA GACCGATGGC 
GAGGAGCCAA GTGGATTGCT GAAGAACTTC CAAGATATCA TGCGAAAGCA AAGACCAGAA 
AGAGTGTCCC ATCTTCTTCA AGATAATTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT 
CGTTTCTTTG AGAAGAAAAT TGATGAGAAA AAAAATGACC ACACCTATCG AGTTTTCAAA 
ACCGTGAACC GGAGGGCACA CATCTTCCCC ATGGCGGATG ACTACTCAGA CTCCCTCATC 
ACCAAAAAGC AGGTGTCGGT TTGGTGTAGC AATGACTACC TAGGAATGAG TCGCCACCCA 
CGGGTGTGTG GGGCAGTCAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGCACT 
AGAAATATTT CTGGAACCAG TAAATTCCAC GTGGACCTGG AGCAGGAGCT TGCTGACCTC 
CATGGGAAAG ATGCAGCACT CCTGTTTTCC TCATGCTTTG TAGCCAATGA CTCGACCCTC 
TTTACCTTGG CTAAGATGAT GCCTGGCTGT GAAATTTACT CTGATGCTGG GAACCACGCC 
TCTATGATCC AGGGGATTCG GAACAGCCGA GTGCCAAAGT ATATCTTCCG CCACAATGAT 
GTCAGCCATC TCAAAGAACT GCTGCAAAGA TCTGACCCTT CCGTCCCCAA GATTGTTGCA 
TTTGAAACCG TCCACTCAAT GGATGGGGCG GTGTGTCCAC TGGAAGAGCT GTGTGACGTT 
GCCCACGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCGGTGGG GCTGTACGGG 
GCTCGAGGCG GAGGGATTGG CGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT 
GGAACACTTG GCAAAGCCTT CGGCTGTGTT GGCGGCTACA TCGCCAGCAC GAGTTCTCTG 
GTTGACACCG TGCGGTCCTA TGCTGCGGGC TTCATCTTCA CCACCTCCCT GCCGCCCATG 
CTGCTGGCTG GAGCTCTGGA GTCTGTGCGG ATCCTGAAGA GCACTGAGGG GCGGGCACTT 
CGCCGCCAGC ACCAGCGCAA CGTCAAGCTC ATGAGGCAGA TGCTAATGGA TGCCGGCCTC 
CCAGTGGTCC ACTGCCCCAG TCACATCATC CCTGTCCGGG TTGCAGATGC TGCTAAAAAC 
ACGGAAGTCT GTGACGAGCT AATGAGCAGA CATAACATCT ATGTCCAAGC AATCAATTAC 
CCGACAGTGC CCCGCGGGGA GGAGCTCCTG CGGATCGCCC CCACCCCTCA CCACACACCA 
CAGATGATGA ACTACTTCCT TGAGAATCTT CTGGCCACGT GGAACCGAGT GGCGCTGGAG 
CTGAAACCAC ATTCCTCAGC TGAGTGTAAC TTCTGTAGGA GGCCGCTGCA TTTCGAAGTG 
ATGAGTGAAA GAGAGAAATC CTACTTCTCA GGCATGAGCA AGCTGGTTTC TGCTCAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101351282 ( XM_023727163.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023582931.1 Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTr164471
Clone ID Related Accession (Same CDS sequence)	XM_023727154.1 , XM_004368595.1 , XM_023727163.1
Accession Version	XM_023727163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	5-aminolevulinate synthase, nonspecific, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443937.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG TTGTTCGCCG CTGCCCATTT TTATCCCGAG TGCCCCAGAC CTTTCTGCAG 
AAGGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGCTGGG 
GCCAAGCCAG CCCCCCGGGC CCTGTCTACT TCAGCAGTTC ACTGCCAGCA GGTCAAAGAA 
ACCCCTCCAG CCAATGAAAA GGACAAAACT GCCAAAGCCG AGGTCCAGCA GGCTTCTGAT 
GGATCCCAGA AGACTCCAGA TGGGGCGCAG CTTCCGTCTG AACACCTCTC CCCTGCCACG 
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTAGCAGCCC AGATGAGCCA GAGGGGCAGC 
AGTGTCTTCC GCAAAGCCAG TCTCGAGCTT CAGGAGGATG TGCAGGAAAT GCATGCCTTG 
AGGAAAGAGG TTGTCCAAAC CTCTGTGAAC CCCCGTGTAA TTAGTGTGAA GACCGATGGC 
GAGGAGCCAA GTGGATTGCT GAAGAACTTC CAAGATATCA TGCGAAAGCA AAGACCAGAA 
AGAGTGTCCC ATCTTCTTCA AGATAATTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT 
CGTTTCTTTG AGAAGAAAAT TGATGAGAAA AAAAATGACC ACACCTATCG AGTTTTCAAA 
ACCGTGAACC GGAGGGCACA CATCTTCCCC ATGGCGGATG ACTACTCAGA CTCCCTCATC 
ACCAAAAAGC AGGTGTCGGT TTGGTGTAGC AATGACTACC TAGGAATGAG TCGCCACCCA 
CGGGTGTGTG GGGCAGTCAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGCACT 
AGAAATATTT CTGGAACCAG TAAATTCCAC GTGGACCTGG AGCAGGAGCT TGCTGACCTC 
CATGGGAAAG ATGCAGCACT CCTGTTTTCC TCATGCTTTG TAGCCAATGA CTCGACCCTC 
TTTACCTTGG CTAAGATGAT GCCTGGCTGT GAAATTTACT CTGATGCTGG GAACCACGCC 
TCTATGATCC AGGGGATTCG GAACAGCCGA GTGCCAAAGT ATATCTTCCG CCACAATGAT 
GTCAGCCATC TCAAAGAACT GCTGCAAAGA TCTGACCCTT CCGTCCCCAA GATTGTTGCA 
TTTGAAACCG TCCACTCAAT GGATGGGGCG GTGTGTCCAC TGGAAGAGCT GTGTGACGTT 
GCCCACGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCGGTGGG GCTGTACGGG 
GCTCGAGGCG GAGGGATTGG CGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT 
GGAACACTTG GCAAAGCCTT CGGCTGTGTT GGCGGCTACA TCGCCAGCAC GAGTTCTCTG 
GTTGACACCG TGCGGTCCTA TGCTGCGGGC TTCATCTTCA CCACCTCCCT GCCGCCCATG 
CTGCTGGCTG GAGCTCTGGA GTCTGTGCGG ATCCTGAAGA GCACTGAGGG GCGGGCACTT 
CGCCGCCAGC ACCAGCGCAA CGTCAAGCTC ATGAGGCAGA TGCTAATGGA TGCCGGCCTC 
CCAGTGGTCC ACTGCCCCAG TCACATCATC CCTGTCCGGG TTGCAGATGC TGCTAAAAAC 
ACGGAAGTCT GTGACGAGCT AATGAGCAGA CATAACATCT ATGTCCAAGC AATCAATTAC 
CCGACAGTGC CCCGCGGGGA GGAGCTCCTG CGGATCGCCC CCACCCCTCA CCACACACCA 
CAGATGATGA ACTACTTCCT TGAGAATCTT CTGGCCACGT GGAACCGAGT GGCGCTGGAG 
CTGAAACCAC ATTCCTCAGC TGAGTGTAAC TTCTGTAGGA GGCCGCTGCA TTTCGAAGTG 
ATGAGTGAAA GAGAGAAATC CTACTTCTCA GGCATGAGCA AGCTGGTTTC TGCTCAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023582931.1
CDS	137..2059
Translation	METVVRRCPFLSRVPQTFLQKAGKSLLFYAQNCPKMMEAGAKPAPRALSTSAVHCQQVKETPPANEKDKTAKAEVQQASDGSQKTPDGAQLPSEHLSPATSQGTASKCPFLAAQMSQRGSSVFRKASLELQEDVQEMHALRKEVVQTSVNPRVISVKTDGEEPSGLLKNFQDIMRKQRPERVSHLLQDNLPKSVSTFQYDRFFEKKIDEKKNDHTYRVFKTVNRRAHIFPMADDYSDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMDTLKQHGAGAGGTRNISGTSKFHVDLEQELADLHGKDAALLFSSCFVANDSTLFTLAKMMPGCEIYSDAGNHASMIQGIRNSRVPKYIFRHNDVSHLKELLQRSDPSVPKIVAFETVHSMDGAVCPLEELCDVAHEFGAITFVDEVHAVGLYGARGGGIGDRDGVMPKMDIISGTLGKAFGCVGGYIASTSSLVDTVRSYAAGFIFTTSLPPMLLAGALESVRILKSTEGRALRRQHQRNVKLMRQMLMDAGLPVVHCPSHIIPVRVADAAKNTEVCDELMSRHNIYVQAINYPTVPRGEELLRIAPTPHHTPQMMNYFLENLLATWNRVALELKPHSSAECNFCRRPLHFEVMSEREKSYFSGMSKLVSAQA

Target ORF information:

RefSeq Version	XM_023727163.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023727163.1

ORF Insert Sequence:

ATGGAGACTG TTGTTCGCCG CTGCCCATTT TTATCCCGAG TGCCCCAGAC CTTTCTGCAG 
AAGGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGCTGGG 
GCCAAGCCAG CCCCCCGGGC CCTGTCTACT TCAGCAGTTC ACTGCCAGCA GGTCAAAGAA 
ACCCCTCCAG CCAATGAAAA GGACAAAACT GCCAAAGCCG AGGTCCAGCA GGCTTCTGAT 
GGATCCCAGA AGACTCCAGA TGGGGCGCAG CTTCCGTCTG AACACCTCTC CCCTGCCACG 
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTAGCAGCCC AGATGAGCCA GAGGGGCAGC 
AGTGTCTTCC GCAAAGCCAG TCTCGAGCTT CAGGAGGATG TGCAGGAAAT GCATGCCTTG 
AGGAAAGAGG TTGTCCAAAC CTCTGTGAAC CCCCGTGTAA TTAGTGTGAA GACCGATGGC 
GAGGAGCCAA GTGGATTGCT GAAGAACTTC CAAGATATCA TGCGAAAGCA AAGACCAGAA 
AGAGTGTCCC ATCTTCTTCA AGATAATTTG CCAAAATCTG TTTCCACTTT TCAGTATGAT 
CGTTTCTTTG AGAAGAAAAT TGATGAGAAA AAAAATGACC ACACCTATCG AGTTTTCAAA 
ACCGTGAACC GGAGGGCACA CATCTTCCCC ATGGCGGATG ACTACTCAGA CTCCCTCATC 
ACCAAAAAGC AGGTGTCGGT TTGGTGTAGC AATGACTACC TAGGAATGAG TCGCCACCCA 
CGGGTGTGTG GGGCAGTCAT GGACACTTTG AAACAACATG GTGCTGGGGC AGGTGGCACT 
AGAAATATTT CTGGAACCAG TAAATTCCAC GTGGACCTGG AGCAGGAGCT TGCTGACCTC 
CATGGGAAAG ATGCAGCACT CCTGTTTTCC TCATGCTTTG TAGCCAATGA CTCGACCCTC 
TTTACCTTGG CTAAGATGAT GCCTGGCTGT GAAATTTACT CTGATGCTGG GAACCACGCC 
TCTATGATCC AGGGGATTCG GAACAGCCGA GTGCCAAAGT ATATCTTCCG CCACAATGAT 
GTCAGCCATC TCAAAGAACT GCTGCAAAGA TCTGACCCTT CCGTCCCCAA GATTGTTGCA 
TTTGAAACCG TCCACTCAAT GGATGGGGCG GTGTGTCCAC TGGAAGAGCT GTGTGACGTT 
GCCCACGAGT TTGGAGCAAT CACCTTCGTG GATGAGGTCC ACGCGGTGGG GCTGTACGGG 
GCTCGAGGCG GAGGGATTGG CGATCGGGAT GGAGTCATGC CAAAAATGGA CATCATTTCT 
GGAACACTTG GCAAAGCCTT CGGCTGTGTT GGCGGCTACA TCGCCAGCAC GAGTTCTCTG 
GTTGACACCG TGCGGTCCTA TGCTGCGGGC TTCATCTTCA CCACCTCCCT GCCGCCCATG 
CTGCTGGCTG GAGCTCTGGA GTCTGTGCGG ATCCTGAAGA GCACTGAGGG GCGGGCACTT 
CGCCGCCAGC ACCAGCGCAA CGTCAAGCTC ATGAGGCAGA TGCTAATGGA TGCCGGCCTC 
CCAGTGGTCC ACTGCCCCAG TCACATCATC CCTGTCCGGG TTGCAGATGC TGCTAAAAAC 
ACGGAAGTCT GTGACGAGCT AATGAGCAGA CATAACATCT ATGTCCAAGC AATCAATTAC 
CCGACAGTGC CCCGCGGGGA GGAGCTCCTG CGGATCGCCC CCACCCCTCA CCACACACCA 
CAGATGATGA ACTACTTCCT TGAGAATCTT CTGGCCACGT GGAACCGAGT GGCGCTGGAG 
CTGAAACCAC ATTCCTCAGC TGAGTGTAAC TTCTGTAGGA GGCCGCTGCA TTTCGAAGTG 
ATGAGTGAAA GAGAGAAATC CTACTTCTCA GGCATGAGCA AGCTGGTTTC TGCTCAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101351282 ( XM_023727172.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023582940.1 Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), transcript variant X3, mRNA.

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CloneID	OTr214082
Clone ID Related Accession (Same CDS sequence)	XM_023727172.1
Accession Version	XM_023727172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	5-aminolevulinate synthase, nonspecific, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443937.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG TTGTTCGCCG CTGCCCATTT TTATCCCGAG TGCCCCAGAC CTTTCTGCAG 
AAGGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGCTGGG 
GCCAAGCCAG CCCCCCGGGC CCTGTCTACT TCAGCAGTTC ACTGCCAGCA GGTCAAAGAA 
ACCCCTCCAG CCAATGAAAA GGACAAAACT GCCAAAGCCG AGGTCCAGCA GGCTTCTGAT 
GGATCCCAGA AGACTCCAGA TGGGGCGCAG CTTCCGTCTG AACACCTCTC CCCTGCCACG 
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTAGCAGCCC AGATGAGCCA GAGGGGCAGC 
AGTGTCTTCC GCAAAGCCAG TCTCGAGCTT CAGGAGGATG TGCAGGAAAT GCATGCCTTG 
AGGAAAGCTG TTTCCACTTT TCAGTATGAT CGTTTCTTTG AGAAGAAAAT TGATGAGAAA 
AAAAATGACC ACACCTATCG AGTTTTCAAA ACCGTGAACC GGAGGGCACA CATCTTCCCC 
ATGGCGGATG ACTACTCAGA CTCCCTCATC ACCAAAAAGC AGGTGTCGGT TTGGTGTAGC 
AATGACTACC TAGGAATGAG TCGCCACCCA CGGGTGTGTG GGGCAGTCAT GGACACTTTG 
AAACAACATG GTGCTGGGGC AGGTGGCACT AGAAATATTT CTGGAACCAG TAAATTCCAC 
GTGGACCTGG AGCAGGAGCT TGCTGACCTC CATGGGAAAG ATGCAGCACT CCTGTTTTCC 
TCATGCTTTG TAGCCAATGA CTCGACCCTC TTTACCTTGG CTAAGATGAT GCCTGGCTGT 
GAAATTTACT CTGATGCTGG GAACCACGCC TCTATGATCC AGGGGATTCG GAACAGCCGA 
GTGCCAAAGT ATATCTTCCG CCACAATGAT GTCAGCCATC TCAAAGAACT GCTGCAAAGA 
TCTGACCCTT CCGTCCCCAA GATTGTTGCA TTTGAAACCG TCCACTCAAT GGATGGGGCG 
GTGTGTCCAC TGGAAGAGCT GTGTGACGTT GCCCACGAGT TTGGAGCAAT CACCTTCGTG 
GATGAGGTCC ACGCGGTGGG GCTGTACGGG GCTCGAGGCG GAGGGATTGG CGATCGGGAT 
GGAGTCATGC CAAAAATGGA CATCATTTCT GGAACACTTG GCAAAGCCTT CGGCTGTGTT 
GGCGGCTACA TCGCCAGCAC GAGTTCTCTG GTTGACACCG TGCGGTCCTA TGCTGCGGGC 
TTCATCTTCA CCACCTCCCT GCCGCCCATG CTGCTGGCTG GAGCTCTGGA GTCTGTGCGG 
ATCCTGAAGA GCACTGAGGG GCGGGCACTT CGCCGCCAGC ACCAGCGCAA CGTCAAGCTC 
ATGAGGCAGA TGCTAATGGA TGCCGGCCTC CCAGTGGTCC ACTGCCCCAG TCACATCATC 
CCTGTCCGGG TTGCAGATGC TGCTAAAAAC ACGGAAGTCT GTGACGAGCT AATGAGCAGA 
CATAACATCT ATGTCCAAGC AATCAATTAC CCGACAGTGC CCCGCGGGGA GGAGCTCCTG 
CGGATCGCCC CCACCCCTCA CCACACACCA CAGATGATGA ACTACTTCCT TGAGAATCTT 
CTGGCCACGT GGAACCGAGT GGCGCTGGAG CTGAAACCAC ATTCCTCAGC TGAGTGTAAC 
TTCTGTAGGA GGCCGCTGCA TTTCGAAGTG ATGAGTGAAA GAGAGAAATC CTACTTCTCA 
GGCATGAGCA AGCTGGTTTC TGCTCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023582940.1
CDS	137..1909
Translation	METVVRRCPFLSRVPQTFLQKAGKSLLFYAQNCPKMMEAGAKPAPRALSTSAVHCQQVKETPPANEKDKTAKAEVQQASDGSQKTPDGAQLPSEHLSPATSQGTASKCPFLAAQMSQRGSSVFRKASLELQEDVQEMHALRKAVSTFQYDRFFEKKIDEKKNDHTYRVFKTVNRRAHIFPMADDYSDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMDTLKQHGAGAGGTRNISGTSKFHVDLEQELADLHGKDAALLFSSCFVANDSTLFTLAKMMPGCEIYSDAGNHASMIQGIRNSRVPKYIFRHNDVSHLKELLQRSDPSVPKIVAFETVHSMDGAVCPLEELCDVAHEFGAITFVDEVHAVGLYGARGGGIGDRDGVMPKMDIISGTLGKAFGCVGGYIASTSSLVDTVRSYAAGFIFTTSLPPMLLAGALESVRILKSTEGRALRRQHQRNVKLMRQMLMDAGLPVVHCPSHIIPVRVADAAKNTEVCDELMSRHNIYVQAINYPTVPRGEELLRIAPTPHHTPQMMNYFLENLLATWNRVALELKPHSSAECNFCRRPLHFEVMSEREKSYFSGMSKLVSAQA

Target ORF information:

RefSeq Version	XM_023727172.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023727172.1

ORF Insert Sequence:

ATGGAGACTG TTGTTCGCCG CTGCCCATTT TTATCCCGAG TGCCCCAGAC CTTTCTGCAG 
AAGGCAGGCA AATCTCTGTT GTTCTATGCC CAAAACTGCC CCAAGATGAT GGAAGCTGGG 
GCCAAGCCAG CCCCCCGGGC CCTGTCTACT TCAGCAGTTC ACTGCCAGCA GGTCAAAGAA 
ACCCCTCCAG CCAATGAAAA GGACAAAACT GCCAAAGCCG AGGTCCAGCA GGCTTCTGAT 
GGATCCCAGA AGACTCCAGA TGGGGCGCAG CTTCCGTCTG AACACCTCTC CCCTGCCACG 
AGCCAGGGCA CTGCAAGCAA ATGCCCTTTC CTAGCAGCCC AGATGAGCCA GAGGGGCAGC 
AGTGTCTTCC GCAAAGCCAG TCTCGAGCTT CAGGAGGATG TGCAGGAAAT GCATGCCTTG 
AGGAAAGCTG TTTCCACTTT TCAGTATGAT CGTTTCTTTG AGAAGAAAAT TGATGAGAAA 
AAAAATGACC ACACCTATCG AGTTTTCAAA ACCGTGAACC GGAGGGCACA CATCTTCCCC 
ATGGCGGATG ACTACTCAGA CTCCCTCATC ACCAAAAAGC AGGTGTCGGT TTGGTGTAGC 
AATGACTACC TAGGAATGAG TCGCCACCCA CGGGTGTGTG GGGCAGTCAT GGACACTTTG 
AAACAACATG GTGCTGGGGC AGGTGGCACT AGAAATATTT CTGGAACCAG TAAATTCCAC 
GTGGACCTGG AGCAGGAGCT TGCTGACCTC CATGGGAAAG ATGCAGCACT CCTGTTTTCC 
TCATGCTTTG TAGCCAATGA CTCGACCCTC TTTACCTTGG CTAAGATGAT GCCTGGCTGT 
GAAATTTACT CTGATGCTGG GAACCACGCC TCTATGATCC AGGGGATTCG GAACAGCCGA 
GTGCCAAAGT ATATCTTCCG CCACAATGAT GTCAGCCATC TCAAAGAACT GCTGCAAAGA 
TCTGACCCTT CCGTCCCCAA GATTGTTGCA TTTGAAACCG TCCACTCAAT GGATGGGGCG 
GTGTGTCCAC TGGAAGAGCT GTGTGACGTT GCCCACGAGT TTGGAGCAAT CACCTTCGTG 
GATGAGGTCC ACGCGGTGGG GCTGTACGGG GCTCGAGGCG GAGGGATTGG CGATCGGGAT 
GGAGTCATGC CAAAAATGGA CATCATTTCT GGAACACTTG GCAAAGCCTT CGGCTGTGTT 
GGCGGCTACA TCGCCAGCAC GAGTTCTCTG GTTGACACCG TGCGGTCCTA TGCTGCGGGC 
TTCATCTTCA CCACCTCCCT GCCGCCCATG CTGCTGGCTG GAGCTCTGGA GTCTGTGCGG 
ATCCTGAAGA GCACTGAGGG GCGGGCACTT CGCCGCCAGC ACCAGCGCAA CGTCAAGCTC 
ATGAGGCAGA TGCTAATGGA TGCCGGCCTC CCAGTGGTCC ACTGCCCCAG TCACATCATC 
CCTGTCCGGG TTGCAGATGC TGCTAAAAAC ACGGAAGTCT GTGACGAGCT AATGAGCAGA 
CATAACATCT ATGTCCAAGC AATCAATTAC CCGACAGTGC CCCGCGGGGA GGAGCTCCTG 
CGGATCGCCC CCACCCCTCA CCACACACCA CAGATGATGA ACTACTTCCT TGAGAATCTT 
CTGGCCACGT GGAACCGAGT GGCGCTGGAG CTGAAACCAC ATTCCTCAGC TGAGTGTAAC 
TTCTGTAGGA GGCCGCTGCA TTTCGAAGTG ATGAGTGAAA GAGAGAAATC CTACTTCTCA 
GGCATGAGCA AGCTGGTTTC TGCTCAGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101351282 ( XM_023727180.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023582948.1 Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), transcript variant X4, mRNA.

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CloneID	OTr214083
Clone ID Related Accession (Same CDS sequence)	XM_023727180.1
Accession Version	XM_023727180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	5-aminolevulinate synthase, nonspecific, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443937.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTAC AAGACGATGA ATGTAATCAG TGTCACTGGA TTGTACGTGT AAAAAACGTT 
CGATTGACAA ATGTGTTATG TTTTTACCAC AATTTAAAAA AAAAAATGGA ATGTAGGTCC 
CATTTGCCTC TTGTTACTTT TGTTACAGAG GTTGTCCAAA CCTCTGTGAA CCCCCGTGTA 
ATTAGTGTGA AGACCGATGG CGAGGAGCCA AGTGGATTGC TGAAGAACTT CCAAGATATC 
ATGCGAAAGC AAAGACCAGA AAGAGTGTCC CATCTTCTTC AAGATAATTT GCCAAAATCT 
GTTTCCACTT TTCAGTATGA TCGTTTCTTT GAGAAGAAAA TTGATGAGAA AAAAAATGAC 
CACACCTATC GAGTTTTCAA AACCGTGAAC CGGAGGGCAC ACATCTTCCC CATGGCGGAT 
GACTACTCAG ACTCCCTCAT CACCAAAAAG CAGGTGTCGG TTTGGTGTAG CAATGACTAC 
CTAGGAATGA GTCGCCACCC ACGGGTGTGT GGGGCAGTCA TGGACACTTT GAAACAACAT 
GGTGCTGGGG CAGGTGGCAC TAGAAATATT TCTGGAACCA GTAAATTCCA CGTGGACCTG 
GAGCAGGAGC TTGCTGACCT CCATGGGAAA GATGCAGCAC TCCTGTTTTC CTCATGCTTT 
GTAGCCAATG ACTCGACCCT CTTTACCTTG GCTAAGATGA TGCCTGGCTG TGAAATTTAC 
TCTGATGCTG GGAACCACGC CTCTATGATC CAGGGGATTC GGAACAGCCG AGTGCCAAAG 
TATATCTTCC GCCACAATGA TGTCAGCCAT CTCAAAGAAC TGCTGCAAAG ATCTGACCCT 
TCCGTCCCCA AGATTGTTGC ATTTGAAACC GTCCACTCAA TGGATGGGGC GGTGTGTCCA 
CTGGAAGAGC TGTGTGACGT TGCCCACGAG TTTGGAGCAA TCACCTTCGT GGATGAGGTC 
CACGCGGTGG GGCTGTACGG GGCTCGAGGC GGAGGGATTG GCGATCGGGA TGGAGTCATG 
CCAAAAATGG ACATCATTTC TGGAACACTT GGCAAAGCCT TCGGCTGTGT TGGCGGCTAC 
ATCGCCAGCA CGAGTTCTCT GGTTGACACC GTGCGGTCCT ATGCTGCGGG CTTCATCTTC 
ACCACCTCCC TGCCGCCCAT GCTGCTGGCT GGAGCTCTGG AGTCTGTGCG GATCCTGAAG 
AGCACTGAGG GGCGGGCACT TCGCCGCCAG CACCAGCGCA ACGTCAAGCT CATGAGGCAG 
ATGCTAATGG ATGCCGGCCT CCCAGTGGTC CACTGCCCCA GTCACATCAT CCCTGTCCGG 
GTTGCAGATG CTGCTAAAAA CACGGAAGTC TGTGACGAGC TAATGAGCAG ACATAACATC 
TATGTCCAAG CAATCAATTA CCCGACAGTG CCCCGCGGGG AGGAGCTCCT GCGGATCGCC 
CCCACCCCTC ACCACACACC ACAGATGATG AACTACTTCC TTGAGAATCT TCTGGCCACG 
TGGAACCGAG TGGCGCTGGA GCTGAAACCA CATTCCTCAG CTGAGTGTAA CTTCTGTAGG 
AGGCCGCTGC ATTTCGAAGT GATGAGTGAA AGAGAGAAAT CCTACTTCTC AGGCATGAGC 
AAGCTGGTTT CTGCTCAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023582948.1
CDS	1526..3169
Translation	MIVQDDECNQCHWIVRVKNVRLTNVLCFYHNLKKKMECRSHLPLVTFVTEVVQTSVNPRVISVKTDGEEPSGLLKNFQDIMRKQRPERVSHLLQDNLPKSVSTFQYDRFFEKKIDEKKNDHTYRVFKTVNRRAHIFPMADDYSDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMDTLKQHGAGAGGTRNISGTSKFHVDLEQELADLHGKDAALLFSSCFVANDSTLFTLAKMMPGCEIYSDAGNHASMIQGIRNSRVPKYIFRHNDVSHLKELLQRSDPSVPKIVAFETVHSMDGAVCPLEELCDVAHEFGAITFVDEVHAVGLYGARGGGIGDRDGVMPKMDIISGTLGKAFGCVGGYIASTSSLVDTVRSYAAGFIFTTSLPPMLLAGALESVRILKSTEGRALRRQHQRNVKLMRQMLMDAGLPVVHCPSHIIPVRVADAAKNTEVCDELMSRHNIYVQAINYPTVPRGEELLRIAPTPHHTPQMMNYFLENLLATWNRVALELKPHSSAECNFCRRPLHFEVMSEREKSYFSGMSKLVSAQA

Target ORF information:

RefSeq Version	XM_023727180.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris 5'-aminolevulinate synthase 1 (LOC101351282), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023727180.1

ORF Insert Sequence:

ATGATTGTAC AAGACGATGA ATGTAATCAG TGTCACTGGA TTGTACGTGT AAAAAACGTT 
CGATTGACAA ATGTGTTATG TTTTTACCAC AATTTAAAAA AAAAAATGGA ATGTAGGTCC 
CATTTGCCTC TTGTTACTTT TGTTACAGAG GTTGTCCAAA CCTCTGTGAA CCCCCGTGTA 
ATTAGTGTGA AGACCGATGG CGAGGAGCCA AGTGGATTGC TGAAGAACTT CCAAGATATC 
ATGCGAAAGC AAAGACCAGA AAGAGTGTCC CATCTTCTTC AAGATAATTT GCCAAAATCT 
GTTTCCACTT TTCAGTATGA TCGTTTCTTT GAGAAGAAAA TTGATGAGAA AAAAAATGAC 
CACACCTATC GAGTTTTCAA AACCGTGAAC CGGAGGGCAC ACATCTTCCC CATGGCGGAT 
GACTACTCAG ACTCCCTCAT CACCAAAAAG CAGGTGTCGG TTTGGTGTAG CAATGACTAC 
CTAGGAATGA GTCGCCACCC ACGGGTGTGT GGGGCAGTCA TGGACACTTT GAAACAACAT 
GGTGCTGGGG CAGGTGGCAC TAGAAATATT TCTGGAACCA GTAAATTCCA CGTGGACCTG 
GAGCAGGAGC TTGCTGACCT CCATGGGAAA GATGCAGCAC TCCTGTTTTC CTCATGCTTT 
GTAGCCAATG ACTCGACCCT CTTTACCTTG GCTAAGATGA TGCCTGGCTG TGAAATTTAC 
TCTGATGCTG GGAACCACGC CTCTATGATC CAGGGGATTC GGAACAGCCG AGTGCCAAAG 
TATATCTTCC GCCACAATGA TGTCAGCCAT CTCAAAGAAC TGCTGCAAAG ATCTGACCCT 
TCCGTCCCCA AGATTGTTGC ATTTGAAACC GTCCACTCAA TGGATGGGGC GGTGTGTCCA 
CTGGAAGAGC TGTGTGACGT TGCCCACGAG TTTGGAGCAA TCACCTTCGT GGATGAGGTC 
CACGCGGTGG GGCTGTACGG GGCTCGAGGC GGAGGGATTG GCGATCGGGA TGGAGTCATG 
CCAAAAATGG ACATCATTTC TGGAACACTT GGCAAAGCCT TCGGCTGTGT TGGCGGCTAC 
ATCGCCAGCA CGAGTTCTCT GGTTGACACC GTGCGGTCCT ATGCTGCGGG CTTCATCTTC 
ACCACCTCCC TGCCGCCCAT GCTGCTGGCT GGAGCTCTGG AGTCTGTGCG GATCCTGAAG 
AGCACTGAGG GGCGGGCACT TCGCCGCCAG CACCAGCGCA ACGTCAAGCT CATGAGGCAG 
ATGCTAATGG ATGCCGGCCT CCCAGTGGTC CACTGCCCCA GTCACATCAT CCCTGTCCGG 
GTTGCAGATG CTGCTAAAAA CACGGAAGTC TGTGACGAGC TAATGAGCAG ACATAACATC 
TATGTCCAAG CAATCAATTA CCCGACAGTG CCCCGCGGGG AGGAGCTCCT GCGGATCGCC 
CCCACCCCTC ACCACACACC ACAGATGATG AACTACTTCC TTGAGAATCT TCTGGCCACG 
TGGAACCGAG TGGCGCTGGA GCTGAAACCA CATTCCTCAG CTGAGTGTAA CTTCTGTAGG 
AGGCCGCTGC ATTTCGAAGT GATGAGTGAA AGAGAGAAAT CCTACTTCTC AGGCATGAGC 
AAGCTGGTTT CTGCTCAGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC101351282 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム